quality assurance and quality control plan - Tampa Bay Water

Tampa Bypass Canal/Alafia River Water Supply Projects 

Hydrobiological Monitoring Program 

QUALITY ASSURANCE AND QUALITY CONTROL PLAN 

VERSION 2.1 

Prepared For: 

Tampa Bay Water 

2535 Landmark Drive, Suite 211 

Clearwater, FL 33761-3930 

Prepared By: 

PBS&J 

5300 West Cypress Street 

Tampa, FL 33607 

Subconsultants: 

University of South Florida 

Fish and Wildlife Research Institute 

Terra Environmental Services Inc 

FINAL DRAFT 

April 2008



QUALITY ASSURANCE AND QUALITY CONTROL PLAN 

VERSION 2.1 

Prepared For: 


2535 Landmark Drive, Suite 211 

Clearwater, FL 33761-3930 

Prepared By: 

PBS&J 

5300 West Cypress Street 

Tampa, FL 33607 

Subconsultants: 




FINAL DRAFT 

April 2008

Page i 

Section i 

April 2008 

Version 2.1FINAL DRAFT 

SIGNATURE PAGE 

The assigned parties have read and agree that the included Quality Assurance and Quality 

Control Plan procedures will be utilized to meet the goals of the Tampa Bypass 

Canal/Alafia Water Supply Projects Hydrobiological Monitoring Program. 

____________________________________ 

South Florida Water Management District 

____________________________________ 


__________________________________ 

PBS&J 

__________________________________ 


Marine Science Department 

__________________________________ 


___________________ 

Date 

___________________ 

Date 

__________________ 

Date 

__________________ 

Date 

__________________ 

Date 

__________________________________ 


___________________ 

Date

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Table of Contents 

Signature Page............................................................................................................................. i 

Table of Contents ....................................................................................................................... ii 

List of Tables ........................................................................................................................... viii 

List of Figures..............................................................................................................................x 

List of Appendices.................................................................................................................... xii 

List of Acronyms and Abbreviations .................................................................................... xiii 

Definitions..................................................................................................................................xv 

Glossary .................................................................................................................................. xvii 

1.0 Introduction 

1.1 Overview of Quality Assurance/Quality Control Plan.................................................... 2 

1.2 Regulatory Requirements of HBMP ............................................................................... 2 

1.3 Goals of the HBMP .........................................................................................................3 

1.4 HBMP Scope ................................................................................................................ 4 

1.4.1 Reporting Units...................................................................................................... 4 

1.4.2 Indicators............................................................................................................. 5 

1.4.3. Sampling Design................................................................................................. 5 

1.4.4 Monitoring Objectives ........................................................................................ 6 

1.4.5 Baseline Conditions ............................................................................................ 7 

1.4.6 Determination of Adverse Impact....................................................................... 8 

1.4.7 Additional HBMP Information ........................................................................... 8 

1.5 Statement of Policy.......................................................................................................... 9 

1.6 Project Organization and Responsibility......................................................................... 9 

1.7 Document Organization................................................................................................. 11 

2.0 Overview of Quality Assurance/Quality Control Plan 

2.1 Sampling Procedures and Protocols ................................................................................ 1 

2.2 Sample Custody and Documentation .............................................................................. 1 

2.3 Analytical Procedures...................................................................................................... 2 

2.4 Targets for Precision, Accuracy and Method Detection Limits ...................................... 3 

2.5 Calibration Procedures and Frequency ............................................................................ 3 

2.6 Preventative Maintenance ............................................................................................... 4 

2.7 Quality Control and Assessment of Precision, Accuracy and Method Detection ........... 4 

2.8 Data Reduction, Validation and Storage/Retrieval ......................................................... 5 

2.9 Corrective Action ............................................................................................................ 6 

2.10 Performance and System Audits ..................................................................................... 7 

2.11 Quality Assurance Reporting .......................................................................................... 7 

2.12 Reporting and Analysis ................................................................................................... 7 

2.13 The General Process of Quality Control and Assurance ................................................ 8 

2.13.1 Review Procedures and Protocols...................................................................... 9 

2.13.2 Standard Five-Step Quality Assurance and Control Review Process.............. 11

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2.13.3 Calculations ...................................................................................................... 11 

2.13.4 Documentation.................................................................................................. 12 

2.13.5 Outside Peer Review......................................................................................... 13 

2.13.6 Reviews by Subconsultants............................................................................... 14 

3.0 HBMP Sampling Strategies 

3.1 HBMP Design - General Considerations ........................................................................ 1 

3.2 General Design Criteria Associated with HBMP Study Elements.................................. 2 

3.3 Indicators ......................................................................................................................... 5 

3.3.1 Desirable Indicator Criteria................................................................................. 5 

3.3.2 Critical Indicator Criteria.................................................................................... 5 

3.4 Reporting Units ............................................................................................................... 6 

3.4.1 Lower Hillsborough River .................................................................................. 6 

3.4.2 Lower Alafia River ............................................................................................. 7 

3.4.3 TBC/Palm River.................................................................................................. 7 

3.4.4 McKay Bay ......................................................................................................... 7 

3.4.5 Hillsborough Bay................................................................................................ 8 

3.5 Spatial Randomization ....................................................................................................8 

3.6 Temporal Strata ............................................................................................................. 10 

3.6.1 Lower Hillsborough River ................................................................................ 10 

3.6.2 Lower Alafia River ........................................................................................... 10 

3.6.3 TBC/Palm River................................................................................................ 11 

3.6.4 McKay Bay ....................................................................................................... 11 

4.0 Data Quality and Management 

4.1 Data Quality Objectives ..................................................................................................1 

4.1.1 Representativeness.............................................................................................. 2 

4.1.2 Completeness ...................................................................................................... 2 

4.1.3 Comparability ..................................................................................................... 3 

4.1.4 Accuracy, Precision, and Total Error.................................................................. 3 

4.2 Routine Methods Used to Assess Precision and Accuracy.............................................. 4 

4.2.1 Field Measurements ............................................................................................. 4 

4.2.2 Laboratory Water Quality Parameters ................................................................ 5 

4.3 Method Detection Limits................................................................................................. 5 

4.4 Data Reduction and Validation ....................................................................................... 6 

4.4.1 HBMP Data Sources ........................................................................................... 6 

4.4.2 Outside Data Sources.......................................................................................... 8 

4.4.3 Data Dictionary................................................................................................... 9 

4.5 Data Methodologies....................................................................................................... 10 

4.5.1 Field Data ......................................................................................................... 10 

4.5.2 Laboratory Data ................................................................................................ 11 

4.6 Data Storage .................................................................................................................. 12 

4.6.1 Field Data.......................................................................................................... 12 

4.6.2 Water Chemistry Data....................................................................................... 12 

4.6.3 HBMP Database................................................................................................ 13

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4.7 Electronic Data Records ................................................................................................ 13 

4.8 Performance and System Audits.................................................................................... 14 

4.8.1 Internal Field System Audits ............................................................................. 14 

4.8.2 External Field System Audits ............................................................................ 15 

4.8.3 Internal Laboratory System Audits.................................................................... 15 

4.8.4 External Laboratory Systems and Performance Audits .................................... 15 

5.0 Water Quality and Hydrology 

5.1 Objectives ......................................................................................................................... 1 

5.2 Hydrology/Water Quality Indicators................................................................................. 2 

5.3 Sample Collection Techniques.......................................................................................... 2 

5.3.1 Pre-Mobilization ................................................................................................. 2 

5.3.2 Same Day Field Mobilization .............................................................................. 4 

5.3.3 Surface Water Sampling ...................................................................................... 4 

5.3.4 In-situ Multi-parameter Sonde Surface Water Sampling..................................... 7 

5.4 Duplicates, Field Blanks and Split Sampling .................................................................. 8 

5.5 Field Preservation ............................................................................................................ 9 

5.6 Chlorophyll a and “Field Filtration”............................................................................... 9 

5.7 Sample Container and Equipment Cleaning Procedures ............................................... 11 

5.7.1 Laboratory.......................................................................................................... 11 

5.7.2 Decontamination of Field Equipment................................................................ 11 

5.8 Sample Transportation and Shipping Procedures.......................................................... 11 

5.9 Sample Custody ............................................................................................................. 12 

5.9.1 Objectives .......................................................................................................... 12 

5.9.2 Sample Custody – Overview ............................................................................. 13 

5.10 Field Sample Control Officer......................................................................................... 15 

5.11 Field Custody and Documentation................................................................................. 15 

5.12 Laboratory Sample Control............................................................................................ 17 

5.13 Laboratory Custody ....................................................................................................... 17 

5.14 Preventative Maintenance.............................................................................................. 18 

5.14.1 Field Instruments/Equipment............................................................................. 18 

5.15 Multi-parameter Sonde Operation and Calibration........................................................ 29 

5.15.1 Introduction ....................................................................................................... 29 

5.15.2 Objectives .......................................................................................................... 20 

5.15.3 Procedures.......................................................................................................... 20 

5.16 Hydrology – Permanent Station Continuous Recorders ................................................ 33 

5.16.1 Introduction........................................................................................................ 33 

5.16.2 Continuous Recorders........................................................................................ 34 

5.16.3 Downloading, Calibration, and maintenance..................................................... 35 

5.16.4 Electronics Box, Solar Voltaic Panels, and Batteries ........................................ 35 

5.16.5 Data Loggers...................................................................................................... 36 

5.16.6 Specific Conductance (Conductivity)................................................................ 37 

5.16.7 Water Levels ...................................................................................................... 38

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6.0 Benthic Macroinvertebrates 

6.1 Field Sampling................................................................................................................. 1 

6.1.1 Equipment.............................................................................................................. 1 

6.1.2 Pre-Mobilization.................................................................................................... 2 

6.1.3 Same Day Field Mobilization................................................................................ 3 

6.1.4 Sampling................................................................................................................3 

6.2 Sample Analyses.............................................................................................................. 8 

6.2.1 Macroinvertebrate Analysis................................................................................... 8 

6.2.2 Sediment Analysis............................................................................................... 11 

7.0 Adult and Juvenile Fish .........................................................................................1 

7.1 Project Management........................................................................................................ 1 

7.2 HBMP Adult and Juvenile Fish Monitoring.................................................................... 1 

7.2.1 Site Selection ......................................................................................................... 1 

7.2.2 Sampling Protocol.................................................................................................. 2 

7.2.3 Data Management.................................................................................................. 3 

7.3 Standard Fisheries Independent Monitoring Procedures and Protocols ........................... 3 

7.3.1 Overview of Fisheries-Independent Monitoring Program .................................... 3 

7.3.2 Principal Investigator’s Duties............................................................................... 5 

7.3.2.1 Objectives ............................................................................................. 5 

7.3.2.2 Pre-Sampling Trip ................................................................................. 5 

7.3.2.3 Post-Sampling Trip ................................................................................ 6 

7.3.2.4 Responsibilities...................................................................................... 7 

7.3.3 Stratified-Random Sampling ................................................................................. 7 

7.3.3.1 Objectives .............................................................................................. 8 

7.3.3.2 Procedure ............................................................................................... 8 

7.3.4 Sampling Gears...................................................................................................... 9 

7.3.4.1 21.3-m Center-Bag Seine...................................................................... 9 

7.3.4.2 Center-bag Seine (21.3-m bay seine technique) .................................. 10 

7.3.4.3 21.3-m River Seine Technique ........................................................... 11 

7.3.4.4 6.1-m Otter Trawl ............................................................................... 13 

7.3.5 Field Sampling..................................................................................................... 17 

7.3.5.1 Sample Work Up................................................................................... 17 

7.3.5.2 Subsampling......................................................................................... 21 

7.3.5.3 Instructions for Filling Out Data Sheets and Labels............................ 25 

7.3.5.4 Representative and Unidentified Sample Collection ........................... 34 

7.3.5.5 Hydrolab Operation and Calibration.................................................... 36 

7.3.5.6 Secchi Disk .......................................................................................... 48 

7.3.6 Laboratory Work-Up.......................................................................................... 48 

7.3.6.1 Representative and Unidentified Sample Work-Up and Reference 

Collection ............................................................................................. 48 

7.3.7 Data...................................................................................................................... 51 

7.3.7.1 Data Processing.................................................................................... 51

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8.0 Ichthyoplankton And Zooplankton 

8.1 Project Management ......................................................................................................... 2 

8.2 Key Professional and Technical Staff............................................................................... 2 

8.2.1 Principal Investigator ............................................................................................ 2 

8.2.2 Lab Manager.......................................................................................................... 2 

8.2.3 Research Assistant................................................................................................. 2 

8.2.4 Lab Technician ...................................................................................................... 2 

8.2.5 Field Operations Manager ..................................................................................... 2 

8.2.6 Marine Technician................................................................................................. 2 

8.2.7 Database Manager ................................................................................................. 3 

8.3 Problem Definition/Background....................................................................................... 3 

8.4 Element Description.......................................................................................................... 3 

8.5 Quality Objectives and Criteria for Measurement Data ................................................... 3 

8.6 Special Training................................................................................................................4 

8.7 Documents and Records ................................................................................................... 4 

8.8 Data Generation And Acquisition..................................................................................... 5 

8.8. 1 Sampling Process Design...................................................................................... 5 

8.8.1.1 Minimum Sample Size............................................................................. 5 

8.8.1.2 Collection Dates and Times.................................................................... 5 

8.8.1.3 Collection Locations ............................................................................... 5 

8.8.2 Sampling Methods ................................................................................................. 6 

8.8.3 Sample Handling and Custody............................................................................... 7 

8.8.4 Analytical Methods ............................................................................................... 7 

8.8.5 Quality Control...................................................................................................... 8 

8.8.5.1 Sample Collection................................................................................... 8 

8.8.5.2 Sample Analysis...................................................................................... 9 

8.8.5.3 Instrument/Equipment Inspection and Maintenance ............................. 10 

8.8.5.4 Instrument/Equipment Calibration........................................................ 10 

8.8.5.5 Inspection/Acceptance of Supplies and Consumables.......................... 11 

8.8.5.6 Data Management ................................................................................. 11 

8.9 Scope of Work ................................................................................................................ 12 

8.9.1 QA/QC Plan Preparation...................................................................................... 12 

8.9.2 Zooplankton Sample Collection and Processing ................................................. 12 

8.9.3 Data Management ................................................................................................ 13 

8.9.4 Project Management ............................................................................................ 13 

8.9.5 Meetings............................................................................................................... 14 

9.0 Water-Dependent Birds 

9.1 Introduction....................................................................................................................... 1 

9.2 Monitoring Overview........................................................................................................ 1 

9.3 Field Observations ............................................................................................................ 2 

9.3.1 Pre-Mobilization .................................................................................................... 2 

9.3.2 Survey Preparation Tasks...................................................................................... 2 

9.3.3 Survey Time and Tide Criteria .............................................................................. 3 

9.3.4 Miscellaneous Materials ........................................................................................ 4

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9.4 Avifauna Survey Data Collection .................................................................................... 4 

9.4.1 Survey Day Field Mobilization ............................................................................. 4 

9.4.2 Biotic Data Protocol .............................................................................................. 4 

9.4.3 Biotic Data Recording Logistics............................................................................ 5 

9.4.4 Field Data Recording Procedures .......................................................................... 5 

9.4.4.1 Standard Species Surveys (Low and High Tides).................................... 5 

9.4.4.2 Determination of Mean Feeding Rates .................................................... 6 

9.4.5 Abiotic Data Protocol............................................................................................ 6 

9.5 Instrument & Equipment Maintenance............................................................................. 7 

10.0 Vegetation 

10.1 Introduction..................................................................................................................... 1 

10.2 Organization and Personnel ............................................................................................ 1 

10.3 HBMP Vegetation Monitoring Components .................................................................. 2 

10.3.1 Alafia River Transitional Fixed Station Area Vegetation Sampling................... 2 

10.3.2 Floodplain Vegetation Polygon Mapping ........................................................... 4 

10.3.3 Linear Vegetation Mapping and Shoreline Surveys ........................................... 5 

10.4 GIS Data .......................................................................................................................... 5 

10.4.1 Metadata.............................................................................................................. 5 

10.4.2 File naming ......................................................................................................... 5 

10.4.3 Directory Structure.............................................................................................. 5 

`0.4.4 Map Standards .................................................................................................... 7 

10.4.5 Map Revisions .................................................................................................... 7 

10.4.6 Map Review ........................................................................................................ 7 

10.4.7 Automated Verification Procedures.................................................................... 7 

10.4.8 Electronic Submittal Standards........................................................................... 8 

11.0 References and Relevant Literature 

Appendix A HBMP Program Modifications and Changes to the 

HBMP QA/QC Plan 

Appendix B Data Upload to STORET

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List of Tables 

Section 1.0 

Table 1.1. 

HBMP strata boundaries in the Alafia, Palm, and Hillsborough River 

reporting units .................................................................................................... 12 

Table 1.2. HBMP elements and critical indicators by reporting unit ................................. 13 

Table 1.3. Key PBS&J HBMP Staff................................................................................... 14 

Section 2.0 

Table 2.1. HBMP Quality Control Checklist – Documents................................................ 15 

Table 2.2. HBMP QA/QC – Project Element Review........................................................ 16 

Table 2.3. Hydrobiological Monitoring Program Quality Assurance/Control – Project 

Element Review................................................................................................. 17 

Section 3.0 

Table 3.1. Hillsborough River Strata .................................................................................. 12 

Table 3.2. Sampling program summary for the lower Hillsborough River reporting 

unit ..................................................................................................................... 13 

Table 3.3. Alafia River Strata ............................................................................................. 14 

Table 3.4. Sampling program summary for the lower Alafia River reporting unit ............ 15 

Table 3.5. Palm River Strata (Tampa Bypass Canal) ......................................................... 16 

Table 3.6. Sampling program summary for the TBC/Palm River reporting unit ............... 17 

Table 3.7. McKay Bay Strata.............................................................................................. 18 

Table 3.8. Sampling program summary for the McKay Bay reporting unit....................... 19 

Table 3.9. HBMP strata boundaries in the Alafia, Palm, and Hillsborough 

River reporting units .......................................................................................... 20 

Section 4.0 

Table 4.1. Measurement Quality Objectives for Indicators................................................ 17 

Table 4.2. Standardized Units Of Measure......................................................................... 18 

Table 4.3. Field and Laboratory MDLs for HBMP Water Quality Parameters.................. 19 

Section 5.0 

Table 5.1 HBMP Water Quality Sampling Study Elements.............................................. 40 

Table 5.2 Hydrology/Water Quality Indicators, Units Of Measure, Sources of 

Variability, and Potential Effects of Surface Water Withdrawals ..................... 41 

Table 5.3. Selected critical hydrologic and water quality indicators specified 

for each waterbody............................................................................................. 42 

Table 5.4 Standard Formats & Information Requirements For Field Notebooks.............. 43 

Table 5.5. Required Containers, Preservation, Holding Times and Sample Volumes ....... 44

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Table 5.6 In Situ Hydrolab Water Quality Indicators, Units Of Measure Accuracy, 

Resolution And Range ....................................................................................... 45 

Table 5.7. Temperature (°C) Versus Dissolved 0xygen (mg/l) Saturation at 

760 mm hg ......................................................................................................... 46 

Table 5.8. HBMP Continuous recorder locations and sensors ........................................... 47 

Table 5.9. Specifications for the sensors as reported by manufacturers............................. 47 

Table 5.10. Stevens encoder LCD menu options summary.................................................. 48 

Section 6.0 

Table 6.1. HBMP Benthic Macroinvertebrate Sampling Study Element ........................... 12 

Table 6.2. Benthic Sampling Checklist............................................................................... 13 

Table 6.3. Number of HBMP Benthic-Samples per Month by Reporting Unit ................. 14 

Table 6.4. Outline of Sampling Activities Performed at each Station................................ 15 

Table 6.5. Benthic Indicators, Units of Measure, and Sources of Variability .................... 15 

Section 7.0 

Table 7.1. Summary of Adult and Juvenile Fish HBMP Sampling Element...................... 53 

Table 7.2. Gear Deployment And Types ............................................................................ 54 

Table 7.3. Maximum bag depths and wing depths for 213-m seine techniques................. 54 

Table 7.4. Freshwater Oxygen Solubility with a Barometric Pressure of 760 mm Hg ...... 55 

Section 8.0 

Table 8.1. Form LFD .......................................................................................................... 15 

Table 8.2. Estimates of Organism Number using Three Methods, Presented 

as the Mean and Standard Deviation of Five Independent Trials...................... 16 

Table 8.3. Summary of Ichthyoplankton and Zooplankton HBMP Sampling Elements.... 17 

Section 9.0 

Table 9.1. Avifauna Survey Equipment Checklist................................................................ 8 

Table 9.2. Alafia River (Banks) Bird Survey Data Collection Form: Sample...................... 9 

Table 9.3. Foraging Survey Data Form............................................................................... 10 

Section 10.0 

Table 10.1. Vegetation Indicators, Units of Measure and Sources of Variability .................. 9 

Table 10.2. The Braun-Blanquet Vegetation Classification Scale.......................................... 9 

Table 10.3. Shoreline and Wetland Mapping Classification System.................................... 10

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List of Figures 

Section 1.0 

Figure 1.1 The HBMP spatial reporting units ........................................................................ 15 

Figure 1.2 Organizational Chart for the Tampa Bypass Canal/Alafia River 

Hydrobiological Monitoring Program Implementation........................................ 16 

Section 3.0 

Figure 3.1 Lower Hillsborough River Reporting Unit........................................................... 21 

Figure 3.2 Alafia River Reporting Unit.................................................................................. 22 

Figure 3.3 

Illustration of the Alafia River HBMP strata and extended area for FIM 

monthly fish sampling........................................................................................... 23 

Figure 3.3 The Palm River and McKay Bay Reporting Unit................................................. 23 

Figure 3.4 McKay Bay Reporting Unit.................................................................................. 24 

Figure 3.5 Hillsborough Bay Reporting Unit......................................................................... 25 

Section 4.0 

Figure 4.1 Overview of the HBMP Data Management Process............................................. 20 

Figure 4.2 Overview of the Contents of the HBMP Database ............................................... 21 

Section 5.0 

Figure 5.1 Organizational Chart of Hydrology and Water Quality HBMP elements ............ 49 

Figure 5.2a LoggerNet Startup screen showing CONNECT button........................................ 50 

Figure 5.2b LoggerNet Connect screen.................................................................................... 50 

Figure 5.3 YSI 600R sonde .................................................................................................... 51 

Figure 5.4 Stevens SDI-12 Encoder....................................................................................... 52 

Section 6.0 

Figure 6.1 Organization of the Benthic Macroinvertebrate study element ............................ 16 

Figure 6.2 Basic benthic sampling equipment ....................................................................... 17 

Section 7.0 

Figure 7.1 Project Organization Adult and Juvenile Fishes................................................... 56 

Figure 7.2 Float plan to be faxed to the Marine Patrol on the morning of sampling ............. 57 

Figure 7.3 21.3-m bay seine technique................................................................................... 58 

Figure 7.4 Organism Measurement Techniques..................................................................... 59 

Figure 7.5 Field Data Sheet.................................................................................................... 60 

Figure 7.6 Length Data Sheet................................................................................................. 61 

Figure 7.7 Sample Labels....................................................................................................... 62 

Figure 7.8 Hydrolab Calibration Sheet................................................................................... 63 

Figure 7.9 Representative Sample Work Sheet...................................................................... 64

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Figure 7.10 Identification Sample Work Sheet........................................................................ 65 

Section 8.0 

Figure 8.1 Organization of the USF Ichthyoplankton/Other Zooplankton Monitoring 

Element ................................................................................................................. 18 

Figure 8.2 Pairing of beakers and labeled cod-end jars in staging area ................................. 19 

Section 9.0 

Figure 9.1 The location of the Alafia Banks monitoring site................................................. 11 

Figure 9.2 Organization of the Water-Dependent Bird element ............................................ 12 

Section 10.0 

Figure 10.1 Organization of Vegetation Elements................................................................... 11

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List of Section Appendices 

Appendix 3-A 

Appendix 4-A 

Appendix 4-B 

Appendix 5-A 

Appendix 5-B 

Appendix 5-C 

Appendix 6-A 

Appendix 6-B 

Appendix 7-A 

Appendix 8-A 

Appendix 9-A 

Appendix 9-B 

Appendix 10-A 

Appendix 10-B 

Appendix 10-C 

Detailed Maps of the HBMP Strata. 

Applicable DEP/EPA Data Qualifier Codes 

Data Dictionary for each of the HBMP Study Components 

Hydrolab Diagrams and Forms 

LoggerNet Datalogger Support Software Instruction Manual 

ACT Performance Verification Statement for YSI Rapid Pulse 

Dissolved Oxygen Sensor 

Example Forms 

Benthic Macroinvertebrates Collected by HBMP 

Code Lists for Data Collection 

Master Ichthyoplankton and Zooplankton HBMP Species List 

Example of Equipment Repair Form 

Bird Species Observed at the HBMP Stations 

Example of Vegetation Monitoring Forms 

Plant Species Observed at the HBMP Fixed Stations 

HBMP Alafia River Vegetation Monitoring Fixed Station Locations

Page xiii 

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List Acronyms and Abbreviations 

AGWMP 

BACI 

DMS 

DOC 

DO 

EAV 

EMAP 

EPA 

EPCHC 

FDEP 

FFWCC 

FMRI 

GIS 

HBMP 

HTML 

LAR 

LHR 

LPR 

NOS 

NRC 

NWS 

Ambient Ground Water Monitoring Program 

Before/After/Control/Impact 

Department of Marine Sciences 

Dissolved Organic Carbon 

Dissolved Oxygen 

Emergent Aquatic Vegetation 

Environmental Monitoring and Assessment Program 

Environmental Protection Agency (U.S.) 

Environmental Protection Commission of Hillsborough County 

Florida Department of Environmental Protection 

Florida Fish and Wildlife Conservation Commission 

Florida Marine Research Institute 

Geographic Information System 



Hyper Text Makeup Language 

Lower Alafia River 

Lower Hillsborough River 

Lower Palm River 

National Oceanic Service 

National Research Council 

National Weather Service

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PBS&J 

QA/QC 

RKm 

SAV 

SAS 

SOP 

SWAMP 

SWFWMD 

SWIM 

TBC 

TBW 

TOC 

TSS 

USF 

USGS 

WUP 

Post, Buckley, Schuh, and Jernigan, Inc. 

Quality Assurance/ Quality Control 

River Kilometer 

Submerged Aquatic Vegetation 

software system developed by the SAS Institute, Cary, NC. 

Standard Operating Procedures 

Surface Water Monitoring Program 

Southwest Florida Water Management District 

Surface Water Improvement and Management 

Tampa Bypass Canal 


Total Organic Carbon 

Total Suspended Solids 


U.S. Geological Survey 

Water Use Permit

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Definitions 

Coordination Review - A review of combined work elements to identify and resolve any 

conflicts that may exist among the various project elements. 

Deliverables - All written and electronic documents and reports which are products of 

professional service, and which are identified to be furnished to SWFWMD as permit 

conditions and within the scope of contract services. 

Program Manager- The Program Manager is responsible for communicating and 

enforcing compliance with all provisions of the Project Quality Control Plan. 

Project Manager - The project team leader responsible for the overall administration, 

management, production coordination. The Project Manager will serve as the primary 

point of contact for all project elements with both Tampa Bay Water and SWFWMD. 

Project Quality Assurance/Quality Control Officer - The Senior Manager with 

project-wide responsibility for administering the Quality Assurance and Control Plan. 

Project Team - Technical and support staff and sub-consultants responsible for 

completion of each HBMP project element and tasks as required by Tampa Bay Water. 

Team member activities are under the direct supervision and direction of the Project 

Manager. 

QC Review - The process for reviewing and correcting work products before they are 

released as Final Work or Task Products to Tampa Bay Water (and/or subsequently to 

SWFWMD). 

QC Reviewers - The designated project team member responsible for performing quality 

control review associated with tasks in accordance with the Project QA/QC Plan; the QC 

Reviewers have the necessary technical knowledge and qualifications to serve as the 

Lead Technical Professional for the work products being reviewed. 

Quality Assurance - Procedures to determine that quality control is being or has been 

performed effectively and appropriately. 

Quality Assurance/Quality Control Plan - A document that details the activities, staff, 

specific methods and procedures for each of the data gathering, reduction and reporting 

element of the HBMP. In addition, this project specific plan prescribes specific QA/QC 

procedures and schedules for each project element.

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Quality Control - Prescribed procedures by which all work elements and products are 

standardized (to the greatest extent possible) reviewed and, where necessary, brought into 

compliance to conform to permit conditions, professional standards, contractual 

obligations, and client commitments. 

Subconsultant - A firm or individual working under contract to PBS&J to provide 

responsible technical services on a project under PBS&J's direction.

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Glossary 

Alpha - the reference probability or significance level chosen which controls the 

probability making a type III error. 

Archived Data Set - includes the original version of the raw data set, verified data set 

and final version of the validated set. 

Benthic Macroinvertebrate Epifauna - animals living on the surfaces of sediments, 

plants, or other surfaces. 

Benthos - organisms associated with the sediment-water interface. 

Data Dictionary - 

provides definitions of all variables in the HBMP data base. 

Focus Group -the group of representatives of numerous federal, state, and local 

environmental regulatory and resource management agencies, as well as national 

and local environmental activist organizations who provided input to the HBMP 

during public workshops. 

Habitat - physical extent of area available, including vegetation, bottom type and 

shoreline characteristics. 

Header Record - portion of the data set which contains the information necessary for 

linking all data, spatially and temporally. 

Hypoxia - low dissolved oxygen conditions. 

In Situ - under natural conditions; typically refers to measures made in the field as 

opposed to in the library. 

Indicator - units of measure that describe the status of the statistical populations, or 

subpopulations, of interest, usually in response to some environmental factor. 

Infauna - animals living within the sediments. 

Inset Reporting Unit - a subset of a larger reporting unit in area, but with adequate 

samples to be used as a reporting unit alone. 

Macroinvertebrate - animals typically retained on 500 micron (>0.5 mm) mesh sieves 

Monitoring Element - groups of indicators of environmental change.

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Monitoring Objectives - developed as the first step in the HBMP design process and 

used to pose questions which are meaningful to the public and provide the basis 

for scientific investigation. 

Nonprobabilistic Sampling - alternatives to probability sampling that are not strictly 

random. 

Percent Cover - the estimated proportion of a sample quadrat in which a particular 

species or taxon. 

Population - the aggregate from which the sample is chosen. 

Probabilistic Sampling - sampling strategy in which samples are selected through a 

random process and each sample receives the appropriate probability of being 

sampled. 

Raw Data Set - the initial data set compiled following primary data collection and initial 

quality assurance checks. 

Reporting Unit - the spatial and temporal extent of the target population, i.e. that portion 

of the study area about which inferences can be made. 

Sampling Population - that portion of the total population from which samples are 

selected. 

Spatial Stratification - allocation of samples to ensure that key sources of spatial 

variation within that area are accommodated. 

Species Composition - the list of occurring species in a sample; it is the list of species 

found in either a sample or a population. 

Species Richness - typically refers to the number of species in a sample and can be 

applied to other taxonomic hierarchies, e.g. taxa richness. 

Stratified Random Sampling - common sampling strategy in which random samples are 

taken from subpopulations or strata of the target population. 

Stratum - a subpopulation of the target population that results from dividing the sampled 

population either spatially or temporally; these strata are non-overlapping and 

together comprise the whole population. 

Subpopulation - see stratum. 

Target Population - the population about which inferences are to be made or 

information is desired. 

Temporal Stratification - allocation of samples to ensure that key sources of temporal

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variation within that area are accommodated. 

Type I Error - rejection of the null hypothesis when it is actually true. 

Type II Error - failure to reject the null hypothesis when it is false. 

Validated Data Set - final data set resulting from review of the verified data set which 

can include cross comparisons between field and laboratory data or between 

taxonomic experts. 

Verified Data Set - a data set resulting from error and range checking of the raw data 

set. 

Zooplankton - the animal components of the plankton community that are generally 

recognized to move passively in the water.

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Version 2.1 FINAL DRAFT 


This Quality Assurance/Quality Control Plan (QA/QC Plan) is one of two documents that 

describe the sampling protocol for Tampa Bay Water’s Hydrobiological Monitoring 

Program (HBMP). The HBMP is required to meet conditions of certain Water Use 

Permits issued to Tampa Bay Water by the Southwest Florida Water Management 

District (SWFWMD) for the Tampa Bypass Canal/Hillsborough River and Alafia River 

Water Supply Projects. The HBMP monitors conditions in four waterbodies in 

Hillsborough County, Florida: 1) the lower Alafia River; 2) the Tampa Bypass Canal 

(also known as the Palm River); 3) McKay Bay; and 4) the lower reaches of the 

Hillsborough River (below the City of Tampa Dam). 

In May 1999, Tampa Bay Water selected PBS&J to design and implement the HBMP. 

A consensus-based process was used to develop the program. This approach created a 

coordinated, interactive forum from which critical input was provided by a team of 

consultant and university experts, as well as representatives of federal, state, and local 

environmental regulatory and resource management agencies, and various environmental 

organizations. This cooperative body was subsequently designated as the HBMP Focus 

Group. The consensus-based process allowed for input into the HBMP design by all 

interested parties, resulting in a more robust technical design, and an enhanced level of 

interagency and intergovernmental communication. This group also meets annually to 

review data collected and potential monitoring program changes. 

The HBMP design document, Tampa Bypass Canal/Alafia River Water Supply Projects 

Hydrobiological Monitoring Program – Final Report (PBS&J, 2000), provides an 

overview of the design process and specifications for the monitoring program. This 

QA/QC Plan provides more detailed descriptions of the monitoring program and methods 

and procedures for implementation. 

Four separate entities conduct sampling and analysis (PBS&J, University of South 

Florida (USF), Florida Fish and Wildlife Research Institute (FWRI), and Terra 

Environmental Services, Inc.). Each of these entities has individual QA/QC plans that 

are consistent with this Plan and are incorporated by reference. 

When changes to the HBMP are warranted based on sampling conditions or other 

requirements, they are reviewed by the Focus Group and approved by the SWFWMD. 

The original design document is not modified to reflect these changes, but the QA/QC 

Plan is updated as needed to account for modifications to the monitoring program. All 

modifications to the initial HBMP design are noted in Appendix A of this QA/QC Plan. 

This document is Version 2.1 of the HBMP QA/QC Plan.


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One of the most important aspects of a monitoring program is the development of 

project-specific protocols and procedures that assure: 

• The quality of all data collected is both known and acceptable; 

• All study element methods are documented and standardized (to the greatest extent 

possible) to other HBMP data sources so that statistically valid comparisons can be 

made among data collected by different project elements; 

• Both the accuracy and precision of all data sources are known, measured, and 

documented throughout the project to ensure compatibility; 

• Known potential sources of error are identified and documented; and 

• Procedures are established by which errors are quickly identified and corrective 

actions taken to minimize data losses (e.g., analyzing laboratory samples quickly so 

that questionable sample results can be re-analyzed within designated holding times). 

Implementation of this QA/QC Plan will ensure that the project meets these goals. The 

QA/QC Plan documents the overall project approach, the rationale behind specific 

monitoring elements, the study element objectives, and overall project objectives. Items 

specific to the HBMP study design that are presented as part of this QA/QC Plan include: 

• Site study maps; 

• Lists of sampling methods and frequencies for all variables to be sampled; 

• A detailed, conceptual framework describing all aspects of the monitoring efforts; and 

• Project schedules. 

This section briefly outlines some of the most important aspects of components for each 

of the HBMP elements included in this QA/QC Plan. 

1.2 Regulatory Requirements of HBMP 

Development and implementation of a set of comprehensive hydrobiological monitoring 

programs for the Alafia and Tampa Bypass Canal Water Supply Projects are required as 

conditions of approval for Water Use Permits 2011794.00 (special conditions 8A and 8B) 

and 2011796.00 (special conditions 10A and 10B), respectively. Because of the similar 

schedule for development and the close proximity of the two water supply projects, the 

Southwest Florida Water Management District agreed that a single integrated HBMP

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could be jointly developed to simultaneously address the permit requirements for both 

projects. As specified in the permits, the primary areas within which monitoring and 

evaluation is required include: the lower Alafia River (downstream of Aldermans Ford 

Park); the Tampa Bypass Canal/Palm River below Structure 160; McKay Bay; and the 

lower reaches of the Hillsborough River from the City of Tampa Dam to Upper 

Hillsborough Bay. 

As part of the Water Use Permit review process, all applicants must demonstrate that the 

proposed withdrawals or water uses: 

Will not cause adverse environmental impacts to wetlands, lakes, streams, 

estuaries, fish and wildlife, or other natural resources (40D-2.301(c)). 

The SWFWMD’s Basis for Review for Water Use Permit approvals of withdrawals from 

natural surface waterbodies also includes the following specific performance standards: 

Flow rates shall not deviate from the normal rate and range of fluctuation to the 

extent that: 

A. Water quality, vegetation, and animal populations are adversely impacted 

in streams and estuaries; 

B. Salinity distributions in tidal streams and estuaries are significantly 

altered as a result of withdrawals; or 

C. Recreational use or aesthetic qualities of the resource are adversely 

impacted. 

With regard to the Tampa Bypass Canal and Alafia River Water Supply Projects, 

reasonable assurance that the above cited performance standards would be met was 

provided by Tampa Bay Water during the permit review process. As a condition of 

permit approval, however, the SWFWMD subsequently required that HBMPs be 

developed and implemented to monitor the potential impacts of the projects. Therefore, 

this requirement essentially dictates that Tampa Bay Water must demonstrate continued 

compliance with the SWFWMD’s performance standards following the construction and 

operation of the permitted facilities, for the duration of the permit. 

Modifications to the WUP for the Tampa Bypass Canal Water Supply Project were 

approved by the SWFWMD in August 2007 (20011796.001). These modifications 

increased allowable withdrawal quantities, but did not change conditions related to data 

collection for the required HBMP. 

1.3 Goals of the HBMP 

As required under the SWFWMD’s Basis for Review, the minimum goal of the HBMP is 

to generate information at an appropriate scale and resolution to determine if the 

permitted water supply projects are in compliance with SWFWMD rules. Accordingly, 

the programmatic goal of the HBMP was articulated in the design document:

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The goal of the HBMP is to ensure that, following the implementation of 

the permitted surface water withdrawals, flows in the Tampa Bypass 

Canal, Hillsborough River and Alafia River do not deviate from the 

normal rate and range of fluctuation to the extent that: 

• water quality, vegetation, and animal populations are adversely 

impacted in streams and estuaries; 

• salinity distributions in tidal streams and estuaries are significantly 

altered as a result of withdrawals; or 

• recreational use or aesthetic qualities of the resource are adversely 

impacted. 

In addition to the above stated goals, the Focus Group recommended programmatic 

objectives to address the SWFWMD’s process for evaluating compliance with Water Use 

Permits. Accordingly, the programmatic objectives of the HBMP were articulated by the 

Focus Group as follows: 

The objectives of the HBMP are to: 

• document existing conditions in the potentially affected waterbodies; 

• enable the detection of changed conditions in the potentially affected 

waterbodies; 

• determine if the detected changed conditions are attributable to 

reductions in freshwater inflows; 

• provide a scientifically defensible means to evaluate whether the 

permitted surface water withdrawals are causing or significantly 

contributing to the detected changed conditions; 

• determine whether the detected changed conditions constitute, or 

could result in, unacceptable adverse impacts; and 

• recommend appropriate management actions or operational changes 

designed to eliminate or mitigate unacceptable adverse impacts, if they 

occur or are expected to occur. 

As reflected in these objectives, the overall purpose and scope of the HBMP extend 

beyond just data acquisition, analysis and reporting. The HBMP also incorporates 

programmatic criteria that have been designed to ensure that the permitted withdrawals 

do not result in violations of SWFWMD rules throughout the lifetime of the permits. 

1.4 HBMP Scope 

1.4.1 Reporting Units 

The Focus Group concluded that any potential impacts from the permitted surface water 

withdrawals would likely first be manifested in the river systems where surface water 

withdrawals will take place, and possibly Hillsborough Bay. Therefore, the potentially 

affected waterbodies were defined to include: the lower reaches of the Alafia River (AR);

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the Tampa Bypass Canal/Palm River (PR); McKay Bay (M); and the tidal or lower 

Hillsborough River (HR). These geographic areas of concern, or study areas, were 

subsequently termed as reporting units. Table 1.1 lists the reporting units and their 

respective geographic boundaries. Figure 1.1 shows the location and extent of each of 

the reporting units. 

In addition, the Focus Group concluded that Hillsborough Bay proper could be 

potentially affected by the permitted water supply projects, but that potential impacts 

related to withdrawals should be detected first in the rivers themselves, and that the 

Environmental Protection Commission of Hillsborough County (EPCHC) and the Florida 

Fish and Wildlife Research Institute (FWRI) have programs in place to monitor changes 

in Hillsborough Bay. Data currently being collected in Hillsborough Bay include water 

quality and benthos (EPCHC) and fish (FWRI). 

1.4.2 Indicators 

The HBMP defines three monitoring program elements including hydrology/water 

quality, biota, and habitat/vegetation. For each program element, a list of critical 

indicators was specified. Indicators are units of measure that describe the status of the 

statistical populations or subpopulations of interest, usually in response to some 

environmental stressor. Table 1.2 below lists the critical indicators identified for each of 

the HBMP elements, and their application to the four primary reporting units. Due to 

structural and hydrobiological differences among the four primary reporting units, the list 

of critical indicators adopted for each varies somewhat. For example, color was 

considered important for the TBC/Palm River, McKay Bay, and the lower Alafia River, 

but not for the lower Hillsborough River. 

1.4.3 Sampling Design 

Two sampling designs were considered for application on the HBMP: probability 

sampling, and non-probability sampling. Probability sampling requires that each possible 

sample has a known and equal probability of selection, and the places and/or times to be 

sampled are selected using a random process. Employing this approach requires a 

definition of the set of distinct samples that the program is capable of collecting with 

respect to a specific population. Non-probability sampling, on the other hand, typically 

involves sampling a restricted portion of a population that is readily accessible (e.g., fixed 

station sampling of salinity from a bridge), or the selection of “typical” or 

“representative” sample units that are close to the samplers perception of the average of 

the target population (e.g., sampling only in the middle of a river channel to the exclusion 

of the channel sides). 

A probability sampling approach allows for inferences to be drawn about the target 

populations (e.g., fish abundance in the lower Alafia River), as well as a variety of 

subpopulations (e.g., fish abundance in a particular segment of the lower Alafia River, 

during a particular season) if those subpopulations, or strata, are designed into the

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sampling approach. For practical purposes, strata refer to spatial and/or temporal 

partitions across which the sampling effort is distributed in order to characterize 

subpopulations. The use of strata within a sampling design enhances the power to detect 

differences because it optimizes the design based on the natural variability of the 

indicators being measured. Incorporating strata into the sampling design, and randomly 

selecting sampling locations across the various strata is referred to as a stratified random 

sampling approach. 

The Focus Group considered the ability to characterize the status of, and draw inferences 

about, the various critical indicators in particular reporting units, during particular time 

periods, to be critical to evaluating potential impacts. Therefore, after considering the 

merits of both probability and non-probability sampling designs, it was concluded that a 

probability-based design was best suited to meet most of the programmatic goals and 

objectives of the HBMP. A probability-based sampling design was considered to be most 

appropriate for discerning spatial and temporal variability in the abundance and 

distribution of water quality constituents (e.g., salinity, dissolved oxygen) and biological 

populations (e.g., benthos, plankton, fish) within the potentially affected waterbodies. It 

should be noted, however, that certain other data needs were identified that could best be 

addressed using a non-probability sampling approach (e.g., fixed station measurements of 

water level and bird populations). 

The theory of stratified sampling deals with properties of population estimates derived 

from stratified samples, and with the optimal number of samples needed to obtain a level 

of precision and statistical power adequate to meet defined monitoring objectives. In 

consideration of these factors, a spatial and temporal stratification scheme for the primary 

reporting units was adopted, and the minimum number of samples needed to estimate the 

status of the various indicators within the defined spatial and temporal strata of interest 

was determined. 

1.4.4 Monitoring Objectives 

Once the reporting units and critical indicators were defined, and the sampling design 

was determined, specific monitoring objectives for each of the three HBMP elements 

were articulated and adopted by the HBMP, as listed below: 

Hydrology/Water Quality 

• Estimate the daily freshwater inflows, freshwater withdrawals, and 

water levels in each reporting unit. 

• Estimate the distribution of water quality indicators by reporting unit 

on an appropriate temporal basis. 

Estimates of freshwater inflows and withdrawals, and water levels, are to be made on a 

daily basis. The temporal basis is consistent for all water quality indicators; however, the 

temporal basis differs among the spatial reporting units. All reporting units have

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adequate sample size to ensure both annual and seasonal estimates of water quality 

conditions. 

Biota 

• Estimate by reporting unit on an appropriate temporal basis, the 

species composition (to the lowest practical identifiable level), and 

abundance and distribution of: 

o juvenile and adult fishes; 

o benthic macroinvertebrate infauna and epifauna; and 

o ichthyoplankton and other macrozooplankton. 

• Estimate the species composition (to the lowest practical identifiable 

level) and abundance of water-dependent birds at the Alafia Banks on 

an appropriate temporal basis. 

As with water quality, the temporal basis is consistent for all biotic indicators, however, 

the temporal basis differs among the spatial reporting units. All reporting units have 

adequate sample size to ensure both annual and seasonal estimates of biotic conditions in 

all reporting units. 

Habitat/Vegetation 

• Vegetation 

o Estimate the areal extent and upstream/downstream limits of, 

emergent vegetation communities by reporting unit on a 5-year 

basis. 

o Estimate species composition and relative abundance of 

submerged aquatic vegetation communities in the Alafia River 

on a 5-year basis. 

o Monitor species composition and relative abundance of 

emergent vegetation in fixed locations on the Alafia River on 

an annual basis. 

• Estimate the distribution of sediment grain size and sediment total 

organic matter by reporting unit on an appropriate temporal basis 

(derived from benthic sampling program). 

The temporal basis is consistent for all habitat indicators across all spatial reporting units. 

All reporting units have adequate sample size to ensure annual estimates of habitat 

conditions. 

1.4.5 Baseline Conditions 

The assessment of data collected by the HBMP focuses on detecting differences or trends 

in the status of critical indicators within the delineated reporting units relative to changes 

in freshwater flow attributable to the surface water projects. As such, an assessment of 

trends or change will rely upon the definition of a defensible and meaningful baseline 

against which future conditions will be compared.

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The Focus Group discussed the alternatives to defining baseline conditions. There are two 

basic sources of data available to define baseline conditions. The data collected under the 

proposed HBMP during the pre-operational period will be the most applicable data for 

comparison, given they will have been collected using the same design and protocols 

employed in the post-operational period. However, there are two drawbacks to solely 

using these data. First, the influence of inter- and intra- annual variation in rainfall and 

other meteorological conditions could be such that the pre-operational data are collected 

under unusually wet or dry conditions. Secondly, the estimate of inter-annual variability 

will be limited due to the short pre-operational period. Therefore, the exclusive use of 

pre-operational HBMP data for defining baseline conditions is not technically defensible. 

Existing data collected by other monitoring programs for the reporting units are the 

second source of data available to define baseline conditions. Clearly, these data provide 

a more long-term depiction of conditions, at least for those data that have been collected 

over many years or decades. These data are useful for understanding how variation in 

rainfall and other meteorological conditions affects environmental conditions in the areas 

of concern. However, the existing data are limited in scope. For example, much of the 

biological data available for analysis have only been collected during recent years. Also, 

much of the geographical areas of concern are not currently sampled. Again, as was 

concluded for the pre-operational data collected under the current HBMP, existing data 

have limited value for defining baseline conditions. 

The HBMP Focus Group, therefore, concluded that the most defensible approach to 

defining baseline conditions would entail use of both historical information and data 

collected from existing monitoring programs, and pre-operational data collected as part of 

the HBMP. 

1.4.6 Determination of Adverse Impact 

As mentioned above, the overall scope and purpose of the HBMP extend beyond just data 

acquisition, analysis and reporting. The HBMP also incorporates programmatic criteria 

that have been designed to ensure that the permitted withdrawals do not result in 

violations of SWFWMD rules throughout the duration of the permits. The HBMP 

defines a process by which adverse impact could be determined. This process also 

establishes a hierarchy of management actions that could be implemented in response to 

detected hydrobiological changes in the reporting units to avoid or minimize the full 

realization of an adverse environmental impact resulting from the permitted surface water 

withdrawals. 

1.4.7 Additional HBMP Information 

The HBMP design also addresses several other aspects of a comprehensive monitoring 

program including: methods and protocols; special studies; data management; data 

analysis; quality control; and reporting (see Tampa Bypass Canal/Alafia River Water

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Supply Projects Hydrobiological Monitoring Program – Final Report (PBS&J, 2000)). 

Results of HBMP monitoring efforts are presented in annual data reports as well as multiyear 

interpretive reports submitted to the SWFWMD. 

1.5 Statement of Policy 

As the prime consultant, PBS&J is responsible for the integration of each of the study 

elements that comprise the Tampa Bypass Canal/Alafia River HBMP. The overall 

success of this complex monitoring effort is ultimately dependent upon the dedication of 

each member of the project team to maintaining the highest professional standards of 

quality. 

The project team members are committed to producing the most accurate and precise 

results possible. The intended use of this document is to provide strict QA/QC 

procedures for each of the project elements. Strict conformance and adherence will 

provide the basis of standards for all monitoring and sampling activities as well as 

provide a reference for evaluating and verifying the quality and validity of all data. The 

adoption of the performance standards contained within this document will ensure strict 

conformance with all permit requirements and eliminate, or reduce to the greatest extent 

possible, the collection of unacceptable data. Strict adherence to the procedures and 

protocols presented in this document will ensure that all data generated are correct, 

legally defensible and fulfill all regulatory requirements. 

The specific project sections of this document are also intended to serve as reference 

manuals, training guides, and statement of required procedures and protocols for each of 

the HBMP study elements. Where noted, these specific sections are presented as 

supplements to existing, more generic QA/QC documents which have been adopted by 

each of the parties responsible for those study elements. 

Through the adoption of this QA/QC Plan, all parties have confirmed their commitment 

to the collection, analysis, documentation and verification of the highest quality data 

possible. All project team members and participants realize that such quality is 

paramount to all subsequent evaluations and statistical implications derived from the 

sample data. 

1.6 Project Organization and Responsibility 

This section provides an overview of the integrated approach that will be utilized to 

implement QA/QC procedures and protocols during all aspects of the HBMP. The 

following describes the general goals and objectives of this Quality Assurance and 

Control Plan. In subsequent sections of this document, detailed QA/QC criteria are 

presented for each of the HBMP project study elements.

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• Project Management - an overall organizational chart depicting the structure and 

responsibilities of key staff and subconsultants is presented in Figure 1.2. 

• Key Professional and Technical Staff - Table 1.3 documents the experience, 

capabilities, and project-specific responsibilities of key members of the project team. 

Any significant changes in staff (and/or responsibilities) will be documented during 

subsequent updates of this document. 

• Project Training and Review of Procedures - all members of the project team will 

undergo both initial training in, as well as periodic reviews, of the purpose, methods, 

and protocols of data collection and quality assurance for all project elements with 

which they are associated. Such training and review will emphasize the ongoing need 

for strict adherence to all written protocols and QA/QC procedures. Written records 

of such training and reviews will be maintained as part of standard QA/QC 

documentation. These training and review procedures are viewed as a key 

component necessary to minimize known sources of variability in sample collection 

and data acquisition. 

• Project Quality Control and Assurance Officer - The QA/QC Officer should be 

provided with maximum independence from the direct supervision of day-to-day 

project operations. This individual will actively participate in all aspects of personnel 

training and data source auditing. It will be the QA Officer’s responsibility to 

conduct both scheduled and unannounced audits of all project protocols and 

procedures, and to prepare reports of all findings and document corrective actions 

taken. 

Dr. Ralph Montgomery, senior scientist with PBS&J, currently serves as the overall 

QA/QC Officer of all of the HBMP study elements. The Project QA/QC Officer will 

ultimately be responsible for ensuring that all elements of the QA/QC plan are both 

implemented and followed. It is imperative that the individual selected for this 

responsibility have extensive experience in all aspects of the types of data collection, 

analysis, handling and reduction that will be conducted as part of these HBMP study 

elements. Dr. Montgomery has over 20 years of experience conducting 

environmental and water quality studies in estuarine systems in southwest Florida. He 

has been a QA/QC officer for both field and laboratory studies, and has prepared 

generic and site specific QA/QC plans. In addition, he is currently serving as the 

QA/QC Officer for the ongoing HBMP studies being conducted in the Lower Peace 

River/Upper Charlotte Harbor estuary for the Peace River/Manasota Regional Water 

Supply Authority. 

1.7 Document Organization 

This document is divided into a preface and eleven sections with associated appendices.

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Section 

i Preface 







7.0 Adult and Juvenile Fish 

8.0 Ichthyoplankton and Zooplankton 



11.0 References

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Table 1.1 HBMP strata boundaries in the Alafia River, TBC/Palm River, and 

Hillsborough River reporting units 

Stratum 

Down-Stream 

Limit (km) 

Upstream 

Limit (km) 

Description 

AR-1 0 River mouth 

AR-2 2.33 Near end of no-wake zone above marina 

AR-3 4.67 Approx. 200 m upstream of Alafia Marina 

AR-4 7.00 Near utility crossing between I75 & 301 

AR-5 9.33 Near sharp bend in Alafia River at mouth of Rice Creek 

AR-6 11.67 In straightaway a little over 1 km downstream from Buckhorn Springs 

AR-7 14.00 Near Kings Avenue Boat Ramp 

AR-7 18.50 Approx. 200 m upstream of Bell Shoals Road 

PR-1 2.20 River mouth where northern bank meets natural McKay Bay shoreline 

PR-2 3.95 Approx. 200 m upstream of downstream railroad bridge 

PR-3 5.69 Near sharp bend in river at 68 St South - between Maydell Drive and trailer park 

PR-3 7.44 Just downstream of structure S-160 Dam 

HR-1 0 Platt Street Bridge 

HR-2 2.55 Just downstream of North Blvd. Bridge 

HR-3 5.09 Just upstream of Duran Playground (between Columbus & MLK bridges) 

HR-4 7.64 Approx. 150 m downstream of Hillsborough Ave. Bridge 

HR-5 10.18 Near Epps Park (approx 250 m downstream of Sligh Ave. Bridge) 

HR-6 12.73 Just upstream of I275 Bridge at Sulphur Springs 

HR-6 16.34 Just downstream of Hillsborough River Dam

Table 1.2 HBMP elements and critical indicators by reporting unit 

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HBMP Element Critical Indicators AR PR MB HR HB 

Hydrology/Water 

Quality 

Biota 

Freshwater Withdrawals X X X 

Streamflow X X X 

Water Level X X X X 

Salinity X X X X X 

Conductivity X X X X X 

Temperature X X X X X 

Dissolved Oxygen X X X X X 

Secchi Disk Depth X X X X X 

Chlorophyll-a X X X X X 

Color X X X X 


Ammonium NH4 * * * * * 

Nitrate-Nitrite Nitrogen * * * * * 

Total Kjeldahl Nitrogen * * * * * 

Orthophosphate Phosphorus * * * * * 

Total Phosphorus * * * * * 

Benthic Macroinvertebrate Infauna X X X X X 

Benthic Macroinvertebrate Epifauna X X X X X 

Ichthyoplankton and Zooplankton X X X X 

Adult and Juvenile Fishes X X X X X 

Water-Dependent Birds 

Emergent Aquatic Vegetation X X X X 

Submerged Aquatic Vegetation 

Vegetation/Habitat Sediment Grain Size X X X X 

Sediment Organic Matter X X X X 

*These non-HBMP parameters are sampled as part of other Tampa Bay Water projects. 

X 

X 

X

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Table 1.3 Key PBS&J HBMP Staff 

Name 

Years 

Degrees & Certifications 

Relevant 

Experience 

Anamisis, K. 12 MS Urban & Regional Planning; BS Environmental Science 

Davidowicz, T. 16 BS Biology 

Jordan, J. 1 BS Marine Science 

Latham, P. 21 PhD Ecology & Environmental Science; MS & BS Biology 

Maki, K. 10 MS Marine Science; BS Zoology/Environmental Science 

Mann, T. 3 BS Environmental Science &Policy 

Montgomery, R. 30 PhD Biology; BS Biology/Zoology, 

Robison, D. 27 MS Marine Science; BS Environmental Science; Professional Wetlands 

Scientist 

Willis, A. 6 MPA Environmental Science and Policy; BS Aquatic Biology 

Winter, J. 10 BS Environmental Science 

Woithe, R. 19.5 PhD & MS Ecology & Environmental Science; BA Biology; Professional 

Wetlands Scientist; Certified Ecologist; Graduate Certificate in Wetlands

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Version 2.1 FINAL DRAFT

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SWFWMD 

J. Emery 

Resource Regulation 

Project Supervision 

PBS&J 

D. Robison 

Senior Staff Oversight 


R. McConnell 

Project Manager 

Project Management 

PBS&J 

B. Woithe 

Project Manager 

Figure 1.2 

Organizational Chart 

Tampa Bypass Canal / Alafia River 

Hydrobiological Monitoring 

Program Implementation 

Quality Assurance & Control 

PBS&J 

R. Montgomery 

QA/QC Officer 

Data Management 

PBS&J 

K. Maki 

Data Base Manager 

Water Quality & 

Hydrology 

PBS&J 

B. Woithe 

R. Montgomery 

Benthic 

Macroinvertebrates 

PBS&J 

B. Woithe 

Adult and Juvenile 

Fishes 

FWRI 

T. MacDonald 

Ichthyoplankton and 

Zooplankton 

USF 

E. Peebles 

Water-Dependent 

Birds 

PBS&J 

T. Davidowicz 

Aquatic Vegetation 

PBS&J 

B. Woithe 

Chemistry 

Southern Analytical 

Laboratories 

P. De Andino 

Benthic Taxonomy 

Terra Environmental 

Services, Inc. 

B. Barber 

Sediment Analysis 

Mote Marine Laboratory

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This section provides a general overview of the Quality Assurance/Quality Control Plan 

(QA/QC Plan) and its implementation. Sections 2.1 through 2.11 describe general 

QA/QC concepts for field sampling and sample analysis. Sections 2.12 and 2.13 describe 

general QA/QC concepts for data management and data analysis. 

2.1 Sampling Procedures and Protocols 

One of the primary functions of this QA/QC Plan is the establishment of Standard 

Operating Procedures (SOPs) for all field sampling and laboratory protocols employed 

during the collection and analysis of data under each of the HBMP study elements. The 

protocols and procedures for each HBMP task are presented in Sections 3.0 through 10.0 

of this document. 

To the greatest extent possible, these procedures have been developed to conform to 

those currently used by agencies collecting similar data in the Tampa Bay estuarine 

system. Subsequent revisions of this document will address any changes in these 

procedures that may be made during the course of the programs. Potential differences in 

detection limits, accuracy, and precision of the resulting data that may result from any 

such changes will be specifically addressed. 

General topics within SOPs for individual HBMP elements may include: 

• Detailed specific procedures and protocols (physical, chemical, biological); 

• Specific use and types of field and laboratory equipment; 

• Sampling techniques for the measurement of ambient physical/chemical parameters; 

• Use of automated samplers and recorders (hydrological and meteorological); 

• Sampling of biological communities and sample analysis; 

• Field, equipment, and laboratory duplicates and blanks; 

• Decontamination, cleaning, and maintenance of field equipment; 

• Use of split sampling; and 

• Preservation and holding times. 

Each section also includes those figures, diagrams, and forms needed to provide reference 

guides for each of the HBMP study elements. Other general topics are included within 

each of these sections as appropriate. 

2.2 Sample Custody and Documentation 

All data collected as part of the HBMP must have verifiable trails of documentation for 

each step in their progress, from the time of sample collection through analysis and final 

reporting and archiving. The purpose of sample custody is to provide a clear, traceable

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description of a sample’s history and to identify the parties responsible for each step in 

the process of data collection and analysis. Both originals and copies of all field and 

laboratory sheets and tracking records must be maintained in project files. In each of the 

following sections, procedures specific to each sampling effort are established. Specific 

protocols addressed in these sections include: 

• Documenting the preparation, use, and maintenance of all field sampling equipment; 

• Field records from all sampling efforts and data acquisition; 

• Sample custody, transport, tracking and other documentation; 

• Chain-of-custody records; 

• Sample security, accessibility, and storage; 

• Electronic records; 

• Security and electronic transfer of data; and 

• Data documentation and verification. 

The following sections of the QA/QC Plan include detailed documentation appropriate 

for each of the above items, and address the customized forms required for each of the 

specific types of data gathered as part of the HBMP study elements. 

2.3 Analytical Procedures 

As previously stated, all methods utilized as part of the HBMP conform to the greatest 

extent possible with procedures currently being used by agencies and investigators 

collecting similar data in the Tampa Bay estuarine system. 

• Laboratory – a laboratory certified to analyze each of the required HBMP analytes, 

and having a currently approved general Laboratory QA/QC Plan must be used to do 

all water chemistry analyses. The Laboratory’s QA/QC Plan should, at a minimum, 

include specific information regarding: 

- Maintenance and cleaning of glassware, 

- Laboratory reagents and storage, 

- Cleaning and documentation of sampling containers, 

- Preservation and holding times, 

- Transportation and shipping, 

- Analytical methods by sample matrix, 

- Detection limits by sample matrix, 

- Analysis of duplicates and blanks, and 

- Spike additions and recoveries. 

• Biological - the HBMP study includes the analysis of several types of biological data 

collected in separate monitoring efforts, including the collection of information on 

benthic invertebrates, zooplankton and ichthyoplankton, juvenile and adult fishes, 

vegetation, and wading birds. While some of these data will be gathered directly in

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the field, other data collection efforts will require considerable laboratory sorting and 

taxonomic identification efforts. Appropriate QA/QC documentation associated with 

these efforts should include: 

- Protocols for sample splitting and sorting, 

- Methods of taxonomic identification, 

- Use of reference collections, 

- Use of voucher specimens (except for birds), 

- Methods employed to assure consistency of identification, and 

- Estimation of accuracy (duplication/recounts). 

2.4 Targets for Precision, Accuracy and Method Detection Limits 

As appropriate to each section, tabular forms are presented summarizing the quality 

assurance objectives associated with both field and laboratory data. Whenever possible, 

the values chosen as objectives are based on historical data collected using similar or 

identical methods to those used for each of the HBMP study elements. Such specific 

HBMP data elements include: 

• Field Data Measures 

- Hydrology - gaged flow, rainfall, gage height; 

- In Situ water quality - temperature, specific conductance, pH, dissolved oxygen; 

- Water Column - Secchi disc, light extinction; and 

- Physical - sampling location, time, date, weather conditions, tide stage. 

• Laboratory 

- Chemistry – chlorophyll-a, total suspended solids, ammonia/ammonium nitrogen, 

nitrite+nitrate nitrogen, total Kjeldahl nitrogen, orthophosphorus, total phosphorus. 

• Biological 

- Sampling - vegetation, benthic invertebrates, zooplankton and ichthyoplankton, 

juvenile and adult fishes. 

- Taxonomic identification - vegetation, benthic invertebrate, juvenile fishes, adult 

fishes. 

2.5 Calibration Procedures and Frequency 

Within the study element protocols developed for the QA/QC Plan are detailed 

descriptions for establishing calibration procedures for each piece of analytical equipment 

used as part of each HBMP program element. These protocols specify both the 

procedures to be used as well as the required frequency of documentation. The specific 

elements differ for each piece of field and laboratory equipment, but general topics 

include:

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• The receipt and tracking requirements of standards; 

• The preparation and storage of standards; 

• Initial calibration requirements; 

• Instrument calibration requirements; 

• Criteria for continuing calibration; and 

• Documentation and records. 

2.6 Preventative Maintenance 

Thorough preventative maintenance programs are vital components of any successful 

QA/QC Plan. Without proper cleaning and maintenance, neither field nor laboratory 

equipment can be expected to provide accurate, reliable results. The following are 

described for each key piece of equipment within the sections of this plan describing each 

of the HBMP project elements: 

• Specific, preventative-maintenance procedures and their required frequency; 

• Maintenance documentation procedures for each piece of equipment; and 

• Contingency plans (both short and long-term). 

2.7 Quality Control and Assessment of Precision, Accuracy and 

Method Detection 

Data quality assurance for many parameters depends on the use of quality-control check 

samples for both field and laboratory analytical procedures. Specific elements typically 

include: 

• Quality control check methods; 

• Routines used to assess precision and accuracy; 

• Method detection and practical quantification limits; and 

• Documentation. 

Field quality-control checks are used to confirm the precision of the sampling techniques, 

verify the cleanliness of the sampling equipment and sampling containers, and address 

potential effects of sample handling and transportation. Such checks of field equipment 

are provided by: 

• Field blanks; 

• Replicate samples; 

• Split samples; 

• Equipment blanks; and 

• Trip blanks.

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Quality control checks for laboratory procedures typically utilize the following 

methodologies: 

• Method reagent blanks; 

• Matrix spikes; 

• Check samples; 

• Duplicate samples; and 

• Continuing calibration standards. 

Biological checks of taxonomic identification may utilize: 

• Reference collections; 

• Photographic catalogs; 

• Verification of identifications by outside experts; 

• 10% of samples also counted by second staff member; and 

• 10% of samples counted by outside expert. 

2.8 Data Reduction, Validation and Storage/Retrieval 

Each of the HBMP study elements has somewhat unique requirements that are addressed 

in each specific section. General considerations include: 

• Data Reduction - Physical, in situ data can be directly read from most field 

instrumentation without the need for calculation routines. Examples would be the 

automated temperature compensation by standard multiprobe meters of pH, specific 

conductance and dissolved oxygen. By comparison, laboratory analytical methods 

are generally based on multi-point standard curves and require significant calculations 

to determine appropriate sample-parameter concentrations. In both instances, the 

QA/QC protocols within each of the following sections require specific 

documentation of all raw data; including how and by whom the raw data were 

recorded; and how and by whom any subsequent calculations were made. All field 

entries should be: 1) made with waterproof ink; 2) be in waterproof notebooks; and 3) 

be signed. Any errors should be corrected by crossing a single line through the error, 

writing the corrected value and then initialing the change. 

• Data Validation - In general, there should be at least three levels of responsibility for 

checking both raw data entries and any subsequent calculations. The first level is the 

individual(s) (field technician, chemist, or biologist) who recorded the raw numbers. 

It is his/her responsibility to follow all procedures correctly, perform required quality 

control checks, and report any discrepancies to the QA/QC Officer designated for 

each of the HBMP study elements. It is the initial responsibility of these individuals 

to check all data entries for transcription and other types of errors. All data are to be 

checked against expected ranges, and any discrepancies resolved in a time frame 

conducive to allowing the re-analysis of samples yielding unusual values.

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Next, a second, similar check of all raw data should be undertaken by the immediate 

supervisor and/or study element QA/QC Officer. It is this person’s initial 

responsibility to investigate and determine the cause of any discrepancies. In 

addition, it is the supervisor/manager’s responsibility to determine if collected data 

fall within observed, historic ranges. The Project Quality Assurance Officer is 

ultimately responsible for checking all sample preparation logs, raw data, calculated 

values, and final databases to assure adherence with project protocols. The Project 

QA/QC Officer is also ultimately responsible for checking all calibrations (field and 

laboratory), determining instrument responses, establishing quality control limits, and 

comparing reported values to similar, historical records. The Project QA/QC Officer 

is responsible for presenting all findings to both the Project Manager and Project 

Director. 

• Data Storage and Retrieval - Only after final data validation is complete are results 

to be entered into the permanent, project databases. All preliminary databases, 

(which reflect initial, reported values), any changes to the databases, and 

documentation of who made the changes, and the justifications for changes must also 

be documented and maintained. Permanent record files are to be kept of all field 

notebooks, laboratory notebooks, equipment logs (e.g., calibration records), chemist 

data logs, strip charts, and raw chemistry data files. Backup copies of all records 

should also be made and kept in a separate location. 

2.9 Corrective Action 

Corrective action is undertaken when established criteria for specific quality control 

measures are not met. Such corrective actions may result from: 

• Performance evaluations or system audits; 

• Divergent results from split or duplicate samples; or 

• Comparative studies of both laboratory and/or field data. 

Sample results outside historic ranges may also lead to re-analysis of samples and 

subsequent corrective actions. Corrective action elements may include: 

• Appropriate modifications of acceptance criteria; 

• Increased quality control measures; 

• Identification of problems and corrective actions; and 

• Notification and documentation. 

It is the responsibility of the Project QA/QC Officer to determine if proposed corrective 

actions are adequate and to document all such actions taken.

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2.10 Performance and System Audits 

Periodic checks and reviews of each of the various components of the HBMP program 

will be employed to assure compliance with all procedures and protocols specified in this 

QA/QC Plan. Such QA/QC reviews will not only guarantee compliance with all 

prescribed methods, but they will also afford opportunities to discuss potential changes 

and additions to further reduce potential errors. Based on evaluations of such suggested 

changes, updates to this document will be made on an as-needed basis. Minor changes 

will be noted as sequential point numbers to the Version Number found in the upper right 

hand corner of each page. Major reviews and/or changes in sections of this document 

will be tracked using major version numbers (i.e., Version 1.2 updated to Version 2.1). 

Performance audits are an essential element of any QA/QC Plan. Both field and 

laboratory audits will be conducted to determine and measure compliance with all aspects 

of this QA/QC Plan. In addition, performance audits will be used to evaluate the quality 

of project data with respect to established limits and standards. Appropriate topics are 

further discussed as part of the HBMP study element specific protocols presented in 

Sections 3.0 through 10.0. Topics include: 

• Field audits; 

• Laboratory audits; and 

• Control procedures. 

2.11 Quality Assurance Reporting 

The Project QA/QC officer shall keep detailed records of all HBMP data-related issues 

identified, especially problems resulting in either significant or repeated corrective 

actions, the findings of performance and system audits, and internal procedural reviews. 

Tampa Bay Water will be notified immediately of any substantial issues affecting data 

quality or usability. All other review, audit, and corrective action records will be made 

available to Tampa Bay Water and SWFWMD upon request. 

2.12 Reporting and Analysis 

The scope of this QA/QC Plan is to establish and define specific goals, objectives, 

methods, processes, project staff, organization, and QA/QC requirements that will be met 

during the development and execution of the HBMP required under SWFWMD’s Water 

Use Permits (WUP). Specific elements within this QA/QC Plan may be updated as 

necessary, with the concurrence of Tampa Bay Water’s (and SWFWMD’s) Project 

Manager, as the project proceeds. 

This QA/QC Plan contains the following elements:

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• General information for each of the HBMP project elements, including background 

information with regard to expectations, goals and objectives 

• A list and description of each task and key milestones 

• A list and description of deliverables required for each project element 

• Project schedules for completion of specific programs tasks 

• Staffing, by name, for each project element 

• A schedule for specific QA/QC review 

• Communication procedures, including Tampa Bay Water contact information and 

provisions for progress reports 

• Document and record specifications identifying document status, archive and 

recording procedures 

2.13 The General Process of Quality Control and Assurance 

This QA/QC Plan will have as one of its basic primary tenets the requirement that at least 

two responsible and qualified members of the project team review and agree on the 

correctness of each Project Document, or any other deliverable, before its release to 

Tampa Bay Water as a final work product. To accomplish this, specific Quality 

Control/Assurance reviews and verifications will take place as follows: 

• The project Quality Control Officer will be responsible for compliance of the 

elements within this QA/QC Plan. Within this plan are designated the Lead 

Technical Professionals and QA/QC Reviewers according to the technical 

requirements of the project. Major scheduled reviews are specifically identified as 

work tasks. Any additional review that may be needed during the duration of each 

project element will be integrated into the overall project schedule with the 

concurrence of Tampa Bay Water. 

• This QA/QC Plan will be presented to Tampa Bay Water’s Project Manager and 

discussed with appropriate members of the project team and SWFWMD. 

• As work progresses on each of the defined Project Tasks, the Quality Control 

Reviewers will work as integral members of the project team under the direction of 

the Project Manager. The Project Manager and Quality Assurance Officer will 

ultimately be responsible for seeing that all elements within the Project Quality 

Control Plan are met. 

• During the review process of each deliverable, it will be the responsibility of the Lead 

Technical Professional responsible for completion of that Task to assemble all 

documents necessary for the QA/QC reviewers, and deliver them to the Project 

Manager. 

• Upon completion of the Quality Control reviews, the Project Manager, Quality 

Control Officer and Lead Technical Professionals will perform a coordinated review

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to determine that the work products for each specific task required for each project 

element are compatible, complete and meet both the SWFWMD’s permit 

requirements and the commitments under Tampa Bay Water’s contract. 

• Before release of deliverables to Tampa Bay Water, the Project Manager and Quality 

Control Officer will be responsible for determining that all steps within the required 

Quality Control Procedures have been accomplished. Only after this Quality 

Assurance Verification will deliverables be submitted to Tampa Bay Water. Under 

extenuating circumstances, such as direct requests by authorized Tampa Bay Water 

staff, “Draft” documents which have not completed this internal review process will 

be released “For Information Only.” 

• Quality Control and Assurance documentation, which is developed during the review 

process, will be properly recorded and filed in the Project files in order to 

demonstrate that the requirements of this document have been met. 

Quality Assurance and Control elements within this plan: 

• Specify the requirements for each subconsultant; 

• Identify specific milestones and Tasks conducted during the HBMP where Quality 

Control Reviews will be required; 

• Identify the level of detail to be applied on each review; 

• Identify the QA/QC Reviewers responsible for each project element, as well as the 

Project Manager responsible for verification. 

• Specify logs and other forms that will be utilized by each of the technical staff 

responsible for each project element and the project Quality Control Officer for 

tracking and documenting the Quality Control and Assurance procedures. These 

forms, logs, and reviews will provide documentation and chronicle the scope and 

timing of each stage of quality control and review. 

2.13.1 Review Procedures and Protocols 

Work products which fall within Quality Control and Assurance Protocols include: 

• Calculations 

• Results from various Computerized Software 

• Written Studies and Reports 

• Maps and Figures developed using GIS and other computer software

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This QA/QC Plan specifies the review procedures and protocols to be used during the 

preparation of both Draft and Final work products as well as the responsibilities of the 

Lead Technical Professionals and Reviewers performing such Quality Controls. 

Generally, all Project Team reviews will be performed on each work product before it is 

used as a further basis for associated elements of the study, and prior to submittal to 

either Tampa Bay Water or the SWFWMD. 

Quality Assurance and Control reviews will generally follow a standardized outlined set 

of internal/external procedures. These procedures are purposefully general to afford 

sufficient flexibility so they can be applied to the wide range of deliverable documents 

and work products required by the SWFWMD’s permit conditions for each of the HBMP 

Project Tasks. The checklists contained within this document will be utilized on an 

initial basis for developing standardized review protocols (Table 2.1). It is expected that 

these initial checklists will be modified as required to meet the needs of Tampa Bay 

Water, SWFWMD and the Project Team. 

In each instance, the Quality Control Reviewer(s) will need to know the technical basis 

on which the specific deliverable work products have been produced, including: 1) the 

project's objectives; 2) constraints; and 3) requirements. It will be the responsibility of 

each Lead Technical Professional responsible for each HBMP study element to furnish 

the reviewer(s) with a complete package of background information with the work 

product to be reviewed. This QC review package will include items such as: 

• The specific scope of the project element and tasks to be reviewed. 

• All completed work products. 

• Any specific directives from Tampa Bay Water or the SWFWMD regarding the 

HBMP study elements as required by the specific permit conditions. 

• Criteria, assumptions, technical standards, codes, etc. 

• Exceptions or variances that may have occurred since the initiation of each task and 

documentation of concurrences, as appropriate, by Tampa Bay Water (or the 

SWFWMD). 

• Applicable data. 

• Test computations and/or computer model calibrations. 

• Alternative analyses and selected alternative justification. 

• Previous review comments, if any, and documentation of substantive changes made 

to preceding Draft documents.

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2.13.2 Standard Five-Step Quality Assurance and Control Review Process 

As a general condition, the following five-step process will be used as a basis for 

reviewing work products prepared under this QA/QC Plan for work conducted as part of 

the HBMP Study Elements. 

1. All documents and deliverables will be prepared under the direct supervision of the 

Lead Technical Professional specified as responsible for each HBMP Study Element. 

The document, unattached figures, maps, etc., as well as supporting documentation 

will be delivered to the Project Manager by the responsible Lead Technical 

Professional for distribution to the Reviewer(s). 

2. The Reviewer(s) will mark in “red” (either on hard copy or through the use of 

tracking in software programs such as Microsoft Word) those items that may require 

correction or additional explanation. Marks or comments by the Reviewer(s) should 

be clearly distinguished from those of the originator. The Reviewer(s) will then return 

the document to the Project Manager for review and concurrence with the Lead 

Technical Professional. 

3. The Lead Technical Professional will indicate concurrence as to changes 

recommended by the Reviewer(s). If they cannot initially agree, they resolve the 

differences with a third qualified technical resource. Comments determined to be 

inappropriate, or not applicable, should be crossed out the by the Lead Technical 

Professional and Reviewer(s). 

4. The agreed-upon changes will then be made, reviewed by the Lead Technical 

Professional and a clean (revised) document will be sent back to the Project Manager 

and subsequently to the Reviewer(s) along with the revised (marked up) review 

document for verification of changes. 

5. The Reviewer(s) verifies by a re-review that the appropriate changes were made to 

the document. 

2.13.3 Calculations 

All calculations used in preparing both raw and final data will be reviewed before 

proceeding to subsequent steps or stages of a project task that relies on their accuracy. 

Such a progressive review of calculations will be used to avoid unnecessary "redos" to 

interim and final work products that depend on successive calculations or modeled 

results. 

In general, only accepted, verified software is to be used in technical work, and only 

under the direction of personnel that are skilled in its use. Only software acceptable to 

Tampa Bay Water (and the SWFWMD if applicable) will be used on the project. In the

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absence of specific acceptance, the program may be used only after sufficient verification 

runs have demonstrated the reliability and the limitations of the software and after the 

verification procedure has been documented and reviewed. 

The technical Reviewer(s) must be knowledgeable about the software's capabilities and 

limitations as well as the technical subject to which the software is applied. The QC 

Reviewer must confirm the following: 

• The software is either accepted by Tampa Bay Water (and the SWFWMD) or the 

necessary verification process has been followed, including documentation; 

• The software was properly applied; 

• The input data were accurate and in the format required by the program; and 

• The output meets the test of reasonableness, based on a sufficient number of spot 

checks 

2.13.4 Documentation 

Keeping track of a project’s QA/QC activities and properly documenting and retaining a 

record of these activities is essential to demonstrate compliance with this QA/QC Plan. 

This section establishes standard procedures as minimum standards for such tracking, 

documentation, and retention of the documentation. 

The Project QA/QC Officer will be responsible for tracking Quality Control and Quality 

Assurance activities during the completion of this project. A key element during this 

process will be confirming that the process is properly documented, and compiling and 

retaining the documentation in a central project Quality Control file. During this study, 

the QA/QC Officer will be responsible for maintaining a Log of all Quality Assurance 

and Control Reviews, by Task (Table 2-2) along with a list of actions necessary for 

compliance with the Plan (Table 2.3). These ongoing Logs will further provide a record 

of the status of all Quality Control Reviews in progress over the project’s duration. 

Quality Control files maintained by the QA/QC Officer will include: 

• The most current QA/ QC Plan as well as documentation of previous revisions 

• Copies of all subconsultant Quality Control Plans and/or letters acknowledging 

conformance with this document 

• Approvals and all correspondences with both Tampa Bay Water and the SWFWMD 

regarding this plan and all revisions 

• A Log depicting the status of QA/QC review procedures

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• All completed Quality Control Checklists 

• Quality Control and Assurance Review comments and responses not contained on 

reviewed documents 

• Any outside Peer Review comments and responses not contained on reviewed 

documents 

• Copies of all Quality Control correspondence 

• All additional internal review documentation. 

During all work on each task for each HBMP Project Element, and subsequent Quality 

Control Reviews, the responsible Lead Technical Professionals will compile and retain 

(file) all relevant technical information pertaining to the work for which they are 

responsible. When the five-step Review Process has been completed for each 

Deliverable Task Element, the Lead Technical Professional will gather all documents 

utilized and/or generated by the Reviewer(s) and others involved in the process and 

forward such Quality Control Documentation “Package" to the Project QA/QC Officer 

for the Quality Control Files. The Project Manager will then: 

• Compile all of the Quality Control documentation packages for the project (identified 

in the QC Review Log) 

• Evaluate documentation for completeness, and follow up with the responsible Lead 

Technical Professional to correct any noted deficiencies 

• Incorporate this information into QC file 

Any additional Quality Control documentation arising from further reviews by the 

Project Team, Tampa Bay Water and/or SWFWMD will be added to this file to create a 

complete record of all Project Quality Control and Assurance activities. 

2.13.5 Outside Peer Review 

Peer reviews are a normal element of QA/QC procedures for project elements that 

involve unusual complexity, importance, innovative requirements, or other similar 

"sensitive" characteristics. In this instance it will be through discussions with Tampa Bay 

Water and the SWFWMD to determine which work products, and at what point of 

completion, project designs and/or completed documents will be submitted for external 

review. Under normal circumstances, such Peer Reviews will usually take place after the 

standard Quality Control review processes have been completed and the work products 

have been submitted to both Tampa Bay Water and the SWFWMD. Peer Reviews are

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expected to cover all the technical aspects of the project deliverables. Critical reviews are 

expected to include examination of typical items, such as: 

• Assumptions and criteria; 

• Judgment, methods, and procedures; 

• Methodology of analytical alternatives; 

• Adequacy and accuracy of analyses; 

• Design and economy of proposed actions; and 

• How well conclusions and any recommended actions meet the project objectives. 

2.13.6 Reviews by Subconsultants 

All subconsultants will be required to have comparable Quality Control systems and 

procedures acceptable to PBS&J, Tampa Bay Water and the SWFWMD. If not, the 

subconsultants will revise or develop their procedures to be equivalent to those specified 

within this document. Subconsultants are to follow the approved procedures, document 

their Quality Control activities, and make their documentation available to the PBS&J 

Project Manager for Quality Assurance verification. If substantially different, copies of 

subconsultants’ individual QA/QC Plans will be included as appendices to this QA/QC 

Plan. Subconsultants must submit documentation to PBS&J showing that their work was 

performed in accordance with the final approved QA/QC Plan.

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Table 2.1. HBMP Quality Control Checklist – Documents 

Project Element: _____________________________________________________ 

Project Task: _______________________________________________________ 

QC Review Confirms That: 

Criteria 

Done 

1. The established scope and objectives of the Task have been achieved 

2. Appropriate technical criteria have been utilized 

3. The approach satisfies the expressed goals of the task 

4. Appropriate data have been acquired, referenced and utilized 

5. Data have been properly checked, analyzed and interpreted 

6. Method and procedures used during analytical procedures were appropriate 

7. Assumptions were clearly defined and appropriate 

8. Presented theories are properly supported (and potential flaws identified) 

9. Reasonable alternatives were investigated and weighed 

10. Conclusions presented are reasonable, based on sound professional judgment 

11. Conclusions are supported by presented lines of evidence 

12. The report format meets the criteria established by Tampa Bay Water (and/or 

SWFWMD) 

13. Tables, figures and graphics are clearly presented 

14. The text is grammatically correct and has been checked 

15. Calculations, models and data inputs have been checked for accuracy 

16. Maps and other graphics have been checked for accuracy 

QC Reviewer: __________________ 

Date: _______________________

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Table 2.2. HBMP QA/QC – Project Element Review 

Project Element Task: 

___________________________________________________________________ 

Review: Quarterly Semi-Annual Annual Other 

Review Of Compliance With SOP Protocols And Procedures: 

Findings: 

QA/QC Officer: ___________________ Date: _______________________

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Table 2.3. Hydrobiological Monitoring Program 

Quality Assurance/Control – Project Element Review 

Project Element Task: 

___________________________________________________________________ 

Review: Quarterly Semi-Annual Annual Other 

Actions Necessary for Compliance With SOP Protocols And 

Procedures: 

Required: 

Recommended: 

Lead Technical Professional: __________________________ 

_______________________ 

QA/QC Officer: __________________________________ 

___________________________ 

Date: ____ 

Date:

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This section provides an overview of the sampling techniques and strategies used in the 

HBMP. Sections 3.1 and 3.2 discuss the general design of the program. Section 3.3 

discusses the indicators used in the program. Section 3.4 describes the geographic 

reporting units of the HBMP. Sections 3.5 and 3.6 discuss spatial and temporal 

randomization and considerations within the sampling program. 

3.1 HBMP Design - General Considerations 

Appropriate environmental monitoring programs are critical components of effective 

resource management. According to the National Research Council (1990) effective 

environmental monitoring: 

• Provides the information needed to evaluate the effectiveness of, and to appropriately 

adjust, resource management actions; 

• Provides an early warning system, allowing for lower cost solutions to environmental 

problems; 

• Contributes to the knowledge of ecosystems and how they are affected by human 

activity, and such knowledge allows for the establishment of priorities for 

environmental protection and for the assessment of status and trends; 

• Provides information that helps to answer layperson questions; 

• Is essential for the construction, adjustment, and verification of quantitative predictive 

models, which are an important basis for evaluating, developing, and selecting 

environmental management strategies; 

• Provides resource managers the scientific rationale for setting environmental 

standards; and 

• Determines legal compliance with established environmental standards and 

conditions as set forth in regulatory programs. 

Environmental monitoring programs need to have clearly articulated goals and objectives 

to minimize data gaps and unanswered critical questions. Therefore, monitoring 

programs need to be properly designed at the outset, and monitoring methods 

appropriately applied, if they are to meet the multiple expectations of all those who rely 

on the information generated. Even when monitoring programs are technically sound, it 

is their overall design and institutional context that often limits the usefulness of the

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resulting information. The National Research Council (1990) has identified the following 

factors for sound program design and objective implementation of monitoring programs. 

• The goals and objectives of the monitoring program must be clearly articulated in 

terms that pose questions that are meaningful to the public and that provide the basis 

for scientific investigation. 

• Not only must data be gathered, but attention must also be paid to their management, 

synthesis, interpretation, and analysis. 

• Procedures for quality assurance are needed, including multiple levels of peer review. 

• Well-designed monitoring programs often result in unanswered questions about 

environmental processes or human impacts. Therefore, where feasible, supportive 

research should be provided. 

• Adequate resources are needed not only for data collection but also for detailed 

analysis, evaluation, and reporting over the long term. 

• Programs should be sufficiently flexible to allow for their modification when and 

where changes in conditions or new information suggests the need. 

• Provision should be made to ensure that monitoring information is made available to 

all interested parties in a form that is useful to them. 

3.2 General Design Criteria Associated with HBMP Study Elements 

The initial step undertaken in the development of the HBMP was to define those 

parameters that were thought to provide the greatest opportunities of detection of any 

significant change in each of the potentially affected water bodies. The next step was to 

define the geographical areas where data collection should be implemented. It was 

agreed that the HBMP sampling design should provide technically sound and practical 

methods for collecting the data needed to obtain unbiased population, subpopulation, and 

variance estimates. 

Unbiased population and subpopulation estimates are metrics whose average value, 

taken over all possible samples, is equal to the population parameter value of the metric. 

In other words, the estimate gives the correct value for some measure of the population. 

Here, the term population refers to the totality of individual observations about which 

inferences are to be made within a definitely specified sampling area limited in space and 

time. Cochran et al. (1954) point out that the population to be sampled (the sampled 

population) should coincide with the population about which inferences are to be drawn 

(the target population). Cochran (1977) further states that at times, for reasons of 

practicability or convenience, the sampled population is more restricted than the target

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population. In such cases, it should be noted that conclusions drawn from the sample 

apply to the sampled population. Judgment must be used to determine the extent to 

which the conclusions will also apply to the target population. One way in which this 

problem can be avoided is to ensure at the design phase that the sampled population is 

carefully defined to closely agree with the target population definition. 

A subpopulation is a specific portion of the population, defined either in space or time. 

For example, the subpopulation may be the shallow portion (defined by some specific 

depth) of the waterbody of concern. A subpopulation could also be all measurements 

made in a particular season of the year (e.g., wet or dry season subpopulations). 

Sampling theory was employed to determine the best design for the HBMP sampling 

strategy. There are basically two common types of sampling strategies (Cochran, 1977): 

• Probability Sampling - Employing this approach requires a definition of the set of 

distinct samples that the sampling program is capable of sampling if applied to a 

specific population. Each possible sample must have a known probability of 

selection. The samples are selected by a random process in which each sample 

receives its appropriate probability of being selected. 

• Nonprobability Sampling - Common approaches include: sampling a restricted 

portion of the population that is readily accessible (e.g., fixed station sampling of 

salinity from a bridge); haphazard sample selection without conscious planning; and a 

selection of “typical” or “representative” sample units that are close to the sampler’s 

impression of the average of the target population. 

If conditions are sufficient, each of the methods can provide useful results. However, the 

only way to verify if an estimate is unbiased is to compare it with the actual population 

values or an estimate derived from a probability sampling approach. Even if such a test 

comparison demonstrates that the nonprobability sampling estimate is unbiased, this does 

not necessarily mean that method will continue to give unbiased results under all 

circumstances. For example, future changes in drainage patterns in a watershed may 

cause more or less runoff to enter the waterbody of concern at the same points that it 

currently enters. Therefore, in this example, checks of the unbiased nature of the 

nonprobability sample estimate would have to continue throughout the length of the 

monitoring program. A probability sampling approach will yield unbiased estimates 

regardless of changing conditions. 

Unbiased variance estimates will provide a measure of uncertainty in the population and 

subpopulation estimates. In order to ensure that variance estimates are unbiased, it is 

recommended that the sampling design follow the basic rules for probability sampling 

and variance estimation. Thus, for all elements of the sampling design, at least two 

samples must be collected from each subpopulation for which an unbiased estimate of 

variance is required, and each sampling unit in the subpopulation must have a known, 

non-zero probability of inclusion in the sample. In addition, the pairwise inclusion

Page 4 

Section 3.0 

April 2008 


probabilities of all possible combinations of the two samples must be known and nonzero. 

Logistical constraints may require that some sampling units have a lower inclusion 

probability than others, and can be incorporated into the sampling design, if necessary. 

The inclusion probabilities used to select sampling units will be specified quantitatively, 

introduced as weights in all computations of estimates and associated variances, and 

hence allow all estimates to be unbiased. 

Given the above conditions, and based on the efforts of the HBMP Focus Group, it was 

concluded that a probability-based design was best suited to meet the programmatic goals 

and objectives of the HBMP. It should, however, be noted that several other data needs 

were also identified that would not be best addressed using a probability-based approach. 

These informational needs will be addressed separately as “special studies” (see HBMP 

design document for additional information). 

To implement the design approach, a series of decisions must be made regarding the 

specifics of the monitoring program design. First, specific monitoring objectives must 

be defined. Second, the indicators of the status of the population (or subpopulations) 

must be defined. Third, the reporting units must be defined, with respect to both space 

and time. 

The general probability sampling approach will allow inferences to be drawn not only 

about the target populations but also a variety of subpopulations. As a general rule of 

thumb, if it is desired to draw an inference with regard to a particular subpopulation, then 

that subpopulation or stratum should be designed into the overall monitoring plan 

(Summers and Maddox, 1999). The use of strata within a sampling design enhances the 

power to detect differences because it optimizes the design based on the natural 

variability characteristics of the indicators being measured. Cochran (1977) concludes 

that incorporating strata into the sampling design, referred to as stratified random 

sampling, is a common and indicated technique if: 

• Data of known precision are wanted for certain subpopulations, in which case it is 

advisable to treat each such subdivision as a “population” in its own right; 

• Convenience dictates the use of stratification; 

• Sampling problems differ markedly in different parts of the population; and 

• It is desired to increase the precision of estimates of the characteristics of the 

population as a whole.

Page 5 

Section 3.0 

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3.3 Indicators 

Following the identification of specific measurable monitoring objectives, the next step in 

the monitoring design process is the definition of appropriate indicators. Indicators are 

units of measure that describe the status of the statistical populations, or subpopulations, 

of interest, usually in response to some environmental stressor. The term indicator in 

this context is somewhat analogous to the term parameter, as it is applied in 

environmental monitoring jargon; however, it is broader in scope. 

Indicators can be generally broken down into two classes: 1) desirable indicators; and 2) 

critical indicators. These two classes are characterized as follows: 

3.3.1 Desirable Indicator Criteria 

• Sampling Unit Stable - measurements of the response indicator taken at a sampling 

unit should be stable over the course of the sampling period. 

• Available Method - should have a generally accepted, standardized method of 

measurement that can be applied on a regional scale. 

• Historical Record - has a historical database, or a historical database can be generated 

from acceptable data sources. 

• Retrospective - can be related to past conditions via retrospective analyses. 

• Anticipatory - provides an early warning of widespread changes in ecosystem 

processes or conditions. 

• Cost-Effective - has low incremental cost relative to its information value. 

• New Information - provides new information; does not merely duplicate data already 

collected by other agencies or investigators. 

3.3.2 Critical Indicator Criteria 

• Regionally Responsive - must reflect changes in ecosystem conditions and respond to 

stressors of concern across most resource classes and habitats in a region. 

• Unambiguously Interpretable - must be related to an assessment endpoint or relative 

exposure or habitat variable that forms part of the investigators’ overall conceptual 

model of ecological structure and function. 

• Low Measurement Error - exhibits low measurement error and stability of regional 

cumulative frequency distribution during the index period.

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Section 3.0 

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• Simple Quantification - can be quantified by cost-effective synoptic or automated 

monitoring. 

• Environmental Impact - sampling must have minimal environmental impact. 

• Low Year-to-Year Variability - must have sufficiently low natural inter-annual 

variation to detect ecologically significant changes within a reasonable time frame. 

It is important to note that, with respect to surface water supply projects, all potentially 

applicable response indicators must have either a direct relationship to changes in 

freshwater inflows, or an indirect relationship (e.g., center of fish population 

distribution). Indicators with a direct relationship to freshwater inflows are typically 

physical or chemical in nature (e.g., salinity), and respond more or less instantaneously to 

changing inflows. Indicators with an indirect relationship to freshwater inflows are 

typically biological in nature (e.g., center of fish population distribution), are mediated by 

physical and chemical changes, and generally respond on a slower time scale (e.g., days, 

months, and seasons). 

The HBMP Focus Group deliberated on critical and desirable indicators over the course 

of several workshops, applied the criteria described above, and generated a list of 

potential indicators for each of the three identified monitoring elements: hydrology/water 

quality, biota, and habitat. This list was ultimately reduced to a final recommended suite 

of critical indicators for each monitoring element that is summarized in the following 

subsections. 

3.4 Reporting Units 

The spatial and temporal extents, statistically often referred to as the target population, of 

the sampling program were defined during the development of the HBMP. Five annual 

reporting units were used to define the spatial and temporal extent for each of the 

sampling program elements. These spatial reporting units are: 

• Lower Hillsborough River – Figure 3.1 

• Lower Alafia River – Figures 3.2 and 3.3 

• Palm River (Tampa Bypass Canal) - Figure 3.4 

• McKay Bay – Figure 3.5 

• Hillsborough Bay – Figure 3.6 

3.4.1 Lower Hillsborough River - The lower Hillsborough River (HR) reporting unit 

extends from the mouth of the river at Platt Street to the City of Tampa Dam. This 

distance in river kilometers is 16.34. This spatial reporting unit was divided into six 

strata, five of equal length (2.55 km) below Sulphur Springs, and one of 3.61 km in 

length from Sulphur Springs upstream to the dam. The river kilometer (km) locations of

Page 7 

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April 2008 


the upstream and downstream extents of each stratum are indicated in Table 3.1. These 

strata will be used for all of the HBMP study elements (i.e., water quality, benthos, adult 

& juvenile fish, plankton, and vegetation). A sampling program summary for this area is 

summarized in Table 3.2. 

3.4.2 Lower Alafia River - The current lower Alafia River (AR) spatial reporting unit 

is about 18.5 kilometers long. The estuarine portion of the river extends from the mouth 

to approximately river kilometer 14 (RKm 14). This estuarine portion of the river was 

divided into 6 strata of equal length (2.33 km). The remaining 4.5 kilometers of the 

reporting unit, from kilometer 14 to kilometer 18.5 slightly upstream of Bell Shoals 

Road, is generally considered to be a freshwater system. An additional freshwater 

segment, upstream of the intake (stratum AR-8 RKm 18.5 to 21.0) was removed from the 

HBMP (see the Water Year 2000 Annual Data Report (PBS&J, 2001)). The river 

kilometer locations of the strata boundaries are shown in Table 3.3. 

These strata will be used for all of the HBMP study elements except benthos and 

vegetation. An insert stratum for benthos sampling was defined in consultation with 

Hillsborough County and the SWFWMD. This stratum extends approximately 1 km 

upstream (river meter 13000) and 1 km downstream (river meter 7000) of the freshwater 

interface and includes stations for portions of strata AR4 and AR6 and all of AR5. For 

the vegetation studies, two transitional areas, i.e., areas at the approximate upstream and 

downstream limits of the center of distribution of black needlerush (Juncus roemerianus) 

will be defined as strata. A sampling program summary for this area is summarized in 

Table 3.4. 

3.4.3 TBC/Palm River - The TBC/Palm River (PR) reporting unit extends from the 

mouth upstream to Structure S-160. As indicated in Table 3.5, this spatial reporting unit 

is divided into 3 strata of equal length (1.75 km). The river kilometers of the upstream 

and downstream extents of the strata are shown in Table 3.5. 

In addition to these longitudinal strata, it was also recognized that significant variation in 

both water quality and benthos might exist due to differences in depth. Therefore, both 

shallow and deep water substrata within each longitudinal stratum were defined in the 

Palm River for both water quality and benthos study elements. A sampling program 

summary for this area is summarized in Table 3.6. 

3.4.4 McKay Bay - The McKay Bay (MB) reporting unit extends from the 22 nd Street 

Causeway to the mouth of the Palm River. This spatial reporting unit was divided into 2 

strata: 1) the channel that runs approximately along the centerline of the bay; and 2) the 

shallow remainder of the bay. The channel portion was further divided into 2 strata of 

equal length (1.1 km) as depicted in Table 3.7. 

Sample selection for water quality and benthic sampling locations will be based on a 

hexagonal grid framework, similar to that used by the Environmental Protection 

Commission of Hillsborough County (EPCHC), except using a finer grid scale of

Page 8 

Section 3.0 

April 2008 


approximately 0.2 km 2 . These hexagons were then divided into a 1-m 2 grid and potential 

sample stations established at the 1-m 2 grid nodes. The hexagons, grids, and stations 

were generated by Janicki Environmental. A sampling program summary for this area is 

summarized in Table 3.8. 

3.4.5 Hillsborough Bay - The HBMP conducts limited sampling in Hillsborough Bay 

proper. HBMP data collection sites are located near the mouth of each primary reporting 

unit. In Water Year 2005, the Fisheries Independent Monitoring Program of the Fish and 

Wildlife Research Institute (FWRI, formerly FMRI) began HBMP-specific sampling in 

Hillsborough Bay at the mouth of the Alafia River. The Environmental Protection 

Commission of Hillsborough County (EPCHC) and the FWRI have long-term monitoring 

programs in Hillsborough Bay which are independent of the HBMP. Results from these 

programs comprise the majority of the data reported by the HBMP for Hillsborough Bay. 

3.5 Spatial Randomization 

A randomized method for choosing monthly sampling sites was used for each of the 

spatial strata within each reporting unit. The centerlines of the linear reporting units were 

generated using an ARCINFO function that interpolates intermediate topographic 

contours. The function used the riverbanks as contours and interpolated the centerlines as 

intermediate contours between the banks. The centerlines were then divided into 1-meter 

long segments, and each of these was defined as a potential sampling station and named 

according to its distance from the river mouth and the strata in which it was located. The 

boundaries of the strata are listed in Table 3.9. 

Monthly sampling stations for the linear reporting units are selected by stratum. Each 

station in the stratum is assigned a unique, consecutive, whole number. A series of 

random numbers is then created using the SAS ranuni() statement. These random 

numbers are matched with the numbers assigned to the stations, and the corresponding 

stations are selected. Lists of primary and alternate stations by stratum are provided to the 

field staff. In the event that a primary station cannot be sampled, field staff must choose 

the first alternate station (not the closest station). If an alternate station cannot be 

sampled, field staff must choose the next alternate station in the sequence. 

A “right,” “left,” or “center” (“shallow right,” “shallow left,” or “deep” in the Palm 

River) designation is also chosen in the monthly sample selection process. A “left” 

designation instructs the field staff to take a sample on the left 33% of the river (with left 

defined as the left side looking upstream). A “center” designation instructs the crew to 

sample in the center 33% of the river and a “right” designation in the right 33%. The 

designations are the same for the Palm River except that the “shallow” stations are 

sampled in water less than 2 meters deep. The lateral positions are randomly selected 

using the SAS ranuni() statement. Each portion of a river has an equal chance of being 

selected for any given station. The depth strata of the Palm River are treated as 

substrata. Lateral position is not randomly selected in the deep Palm River substrata. 

“Left” or “right” lateral positions are randomly selected in the Palm River shallow strata.

Page 9 

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Stations for water quality, benthos, and fish sampling are randomly selected each month. 

Stations for ichthyoplankton sampling were randomly selected one time at the beginning 

of the program. These ichthyoplankton stations are used every month and can be 

considered randomly selected, fixed stations. 

There are single, fixed, water quality sampling stations at the mouths of the Alafia and 

Hillsborough rivers. These roughly correspond to EPCHC fixed stations #8 and #52 in 

Hillsborough Bay. The Alafia fixed station is located at the Green #11 channel marker of 

the Alafia River channel. The Hillsborough fixed station is located at the northern Cut D 

Range Marker for the Cut D Channel. This marker is located just west of Pendola Point 

off Port Sutton. 

McKay Bay monthly stations are grouped by hexagon or cell. Initially a cell is randomly 

selected for sampling. Then the stations (primary and alternates) within the selected cell 

are selected in a manner consistent with the technique described above for the linear 

reporting units. Lists of the primary and alternate stations for each selected cell are 

provided to the field staff. There is only one primary station in each cell. If that station 

cannot be sampled, field staff must select the first alternate station in that cell. If an 

alternate station cannot be sampled, crews must select the next alternate station in that 

cell. There are no lateral sample station designations in McKay Bay. 

McKay Bay cells are selected using a method weighted for the number of stations in each 

cell. Each of the McKay Bay cells is the same size, but some of the cells on the edge of 

the bay overlap land areas. These overlap areas do not constitute valid stations. McKay 

Bay cells are selected using a weighted formula in SAS. Each cell is assigned a 

sequential percentage of the values from 0 to 1 in accordance with the proportion of the 

total stations in McKay Bay located in that cell. For instance, if Cell 1 contains 15% of 

the total stations it would be assigned values from 0.00001 to 0.15000 in the formula. If 

Cell 2 contained 5% of the stations in McKay Bay, it would be assigned values from 

0.15001 to 0.20000 in the formula. Though the water quality, benthos, fish, and plankton 

efforts may sample the same cell during a given month, a single sampling element is 

never sampled twice within one cell in a given month.

Page 10 

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3.6 Temporal Strata 

Temporal strata are specific to each of the four reporting units. In general, these strata 

were defined to ensure that the key within-year sources of variation were taken into 

account. Specifically, two sources of within-year variation were considered critical. One 

source dealt with the within-year variation in flows due to the seasonal differences in 

rainfall. Typically, flows are higher in the normal wet season period (July through 

September) when compared to flows observed in the dry season (October through June). 

Flows in the months of April and May in particular can be extremely low due to 

prolonged periods of low rainfall. The second within-year source of expected variation in 

flows would be the manifestation of the permitted withdrawal schedules. Since the 

withdrawal schedules vary as a function of ambient flow, then the reductions in 

freshwater flow due to the withdrawals will not be consistent throughout the year. 

Analysis indicates that the potential for the greatest daily withdrawals should occur 

during the months of July through September. 

Two other sources of temporal variation, tides and diel variability, were discussed during 

the development of the sampling program but were dropped due to other sampling 

criteria and constraints. 

3.6.1 Lower Hillsborough River - The general temporal sampling strategy for the 

lower Hillsborough River ensures that an adequate sample size is available for drawing 

inferences about river-wide status on a quarterly basis. A relatively equal sampling effort 

is proposed for water quality, fish, and plankton sampling. By comparison, the benthic 

sampling program includes two different sampling intensities. Benthic sampling in both 

the wet season (i.e., July through September) and in the late dry season (April through 

June) is greater than that extended during the periods of October through December and 

January through March. Thus, the Lower Hillsborough River sampling strategy will 

allow inferences to be drawn on a quarterly basis for water quality, fish, and plankton; 

and on a wet season/dry season basis for benthos. 

In addition, continuous monitoring (every 15 minutes) of conductivity will be 

implemented at three permanent fixed locations. These stations will provide information 

on short responses in water quality to changes in freshwater flow. Rainfall and flow 

measurements will be determined on a daily basis. Vegetation sampling will be limited 

to aerial photography and shoreline surveys conducted every five years during the month 

of October. 

3.6.2 Lower Alafia River - The temporal sampling strategy for the lower Alafia River 

is very similar to that defined for the lower Hillsborough River. Equal sampling 

intensities will be employed across all months for water quality, benthos, fish, and 

plankton sampling. The sampling intensity will be sufficient to allow meaningful riverwide 

inferences regarding each of these HBMP elements to be drawn on a quarterly 

basis. An additional fish sampling effort will occur once annually in the freshwater 

stratum.

Page 11 

Section 3.0 

April 2008 


The HBMP Focus Group recommended that an inset stratum for benthic sampling be 

specifically addressed, extending approximately 1 km upstream and 1 km downstream of 

the freshwater interface based on an analysis of pre-operational water quality data. 

Benthic sampling in the inset stratum will be sufficiently intense to allow inferences to be 

drawn about the status of this stratum during the wet season. 

Continuous monitoring (every 15 minutes) of conductivity will be implemented at one 

permanent site. In addition, the SWFWMD is currently collecting data from two 

additional fixed sites on the Alafia River. These data will provide information on short 

responses in water quality to changes in freshwater flow. The frequency of rainfall and 

flow measurements will be daily. Vegetation sampling will be limited to aerial 

photography and shoreline surveys conducted every five years during the month of 

October. Emergent aquatic vegetation will be monitored every year in two strata. 

Submerged aquatic vegetation is monitored ever five years in two strata. 

3.6.3 TBC/Palm River - The temporal sampling strategy for the TBC/Palm River (PR) 

focuses on the expected differences in river status that are likely to occur between the wet 

and dry seasons. Sampling for benthos and water quality during the wet season, when 

mean daily withdrawals will be largest, will be more intense than during the dry season. 

To this end, there are two temporal sampling strata for both water quality and benthos, 

with adequate sampling intensity for inferences to be made regarding the status of water 

quality or benthos in the river for both the wet and dry seasons. Equivalent sampling 

intensities will be employed across all months of the year for both fish and zooplankton 

sampling. 

Continuous monitoring (every 15 minutes) of conductivity will be implemented at 

Maydell Drive. This monitoring will provide information on short responses in water 

quality to changes in freshwater flow. The frequency of rainfall and flow measurements 

will be daily. Vegetation sampling will be limited to aerial photography and shoreline 

surveys conducted every five years during the month of October. 

3.6.4 McKay Bay - The temporal sampling strategy for McKay Bay closely follows 

that for the TBC/Palm River and focuses on the expected differences in the status of 

McKay Bay between the wet and dry seasons. Continuous monitoring (every 15 

minutes) of conductivity will be implemented at the 22 nd Street Causeway. Sampling of 

benthos and water quality will be more intense during the wet season, when mean daily 

withdrawals will be largest. Equivalent sampling intensities are to be employed across all 

months for both fish and plankton sampling. 

The sampling schedule will be based on a temporal randomization around a selected day 

within each month. This method will provide estimates of within-temporal stratum 

variability, increase the power of any tests of significance, and also best represent the 

range of antecedent flow conditions observed during this period.

Page 12 

Section 3.0 

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Table 3.1 Hillsborough River Strata 

Stratum Downstream Limit (km) Upstream Limit (km) 

HR 1 0 2.55 

HR 2 2.55 5.09 

HR 3 5.09 7.64 

HR 4 7.64 10.18 

HR 5 10.18 12.73 

HR 6 12.73 16.20

Page 13 

Section 3.0 

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Table 3.2 Sampling program summary for the lower Hillsborough River reporting unit. 

Element 

Spatial Strata 

Month 

Jan. Feb. Mar. Apr. May Jun. Jul. Aug. Sept. Oct. Nov. Dec. 

Total 

Water 

Quality 

6 strata 

1 fixed bay 

station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

72 

12 

Benthos 6 strata 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 144 1 

Fish - 

Adult/Juveniles 2 

6 longitudinal 

strata with 

shallow (seine) 

and deep 

(trawl) 

substrata 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

144 

seines 

72 

trawls 


other Zooplankton 

6 strata 2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

144 

hauls 

Hydrology 

Vegetation/Habitat 

Temperature, conductivity and salinity (bottom and surface) at Sligh Ave., Columbus Ave., and Crosstown Expressway 

Entire 

reporting unit 

aerial 

1 

photo. 3 

shoreline 

1 

survey 3 

Birds None 

1 Only samples collected from January through March, and July through September, are processed; remaining samples are archived. 

2 One trawl is collected in each stratum, HR1 – HR5, each month. Since trawling in stratum HR6 has proven to be prohibitive, the sixth trawl site each month is randomly selected from one of the lower five strata (HR1 – 

HR5). 

3 Conducted every five years.

Page 14 

Section 3.0 

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Table 3.3 

Alafia River Strata 


Outer Alafia 1 NA NA 

AR 1 0 2.33 

AR 2 2.33 4.67 

AR 3 4.67 7.00 

AR 4 7.00 9.33 

AR 5 9.33 11.67 

AR 6 11.67 14.00 

AR 7 14.00 18.50 

AR 8 2 18.50 21.00 

1 The Outer Alafia is the area of Hillsborough Bay outside the mouth of the Alafia River. Fish sampling is 

the only HBMP element conducted in this stratum. 

2 Stratum AR 8 was included in the initial design but has been subsequently dropped from the HBMP 

sampling

Page 15 

Section 3.0 

April 2008 


Table 3.4 Sampling program summary for the lower Alafia River reporting unit. 

Element 


Month 


Total 

6 estuarine strata 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

144 


Quality 

1 fixed bay station 

1 fixed river station 

(profile only) 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

1 station 

12 

12 

1 freshwater 

stratum (AR7) 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

30 

Benthos 


1 inset stratum 

1 freshwater 

stratum (AR7) 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

7/stratum 

3/stratum 

2/stratum 

6/stratum 

3/stratum 

2/stratum 

7/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

1 SCI/ 

stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

144 

20 

36 

1 SCI 

Fish - 

Adult/Juveniles 1 

6 longitudinal, 

estuarine strata with 

shallow (seine) and 

deep (trawl) 

substrata 

1 freshwater 

stratum (AR7) 

Seines 

2 bay strata outside 

of river 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

10 seines/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines & 

2 trawls / 

stratum 

144 

seines 

72 

trawls 

10 

seines 

48 

seines 

48 

trawls 



6 estuarine 2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

144 

hauls 

Hydrology 


Temperature, conductivity and salinity (bottom and surface) 1km downstream of Buckhorn Springs. 

Entire reporting unit 

2 strata 

(transition zones) 

aerial 

photo. 2 

1 

shoreline 

survey 

1 

32 cells/ 

stratum for 

EAV 3 and 

SAV 2 64 

Birds 

Survey of shoreline 

and subtidal flats 

1 1 1 1 1 1 6 

1 

One trawl is collected in each stratum, AR1 – AR5, each month. Since trawling in stratum AR6 has proven to be prohibitive, the sixth trawl site each month is randomly selected from one of the lower four strata (AR1 – 

AR4). While a portion of AR5 can be sampled with trawls, it is excluded from selection for the sixth trawl haul because of the limited amount of area that can be trawled. 

2 

Conducted every five years. 3 Conducted annually.

Page 16 

Section 3.0 

April 2008 


Table 3.5 Palm River Strata (Tampa Bypass Canal) 


PR 1 2.2 3.95 

PR 2 3.95 5.69 

PR 3 5.69 7.44

Page 17 

Section 3.0 

April 2008 


Table 3.6 Sampling program summary for the TBC/Palm River reporting unit. 

Element 


Month 


Total 


Quality 


strata with 

Deep (D) 

channel & 

shallow (S) 

substrata 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

2 D & 

2 S / 

stratum 

2 D & 

2 S / 

stratum 

2 D & 

2 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

90 

1 Fixed Station 

on upstream side 

of S-160 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

12 

Benthos 

3 strata with 

Deep (D) 

channel / shallow 

(S) substrata 

PR1: 1D 

PR2: 1S 

PR3: 1S 

PR1: 1S 

PR2: 1D 

PR3: 1D 

PR1: 1S 

PR2: 1D 

PR3: 1S 

PR1: 1D 

PR2: 1S 

PR3: 1S 

PR1: 1S/1D 

PR2: 1S 

PR3: 1D 

PR1: 1D 

PR2: 1S/1D 

PR3: 1S/1D 

PR1: 2S/2D 

PR2: 2S/2D 

PR3: 1S/1D 

PR1: 1S/1D 

PR2: 2S/2D 

PR3: 2S/2D 

PR1: 2S/2D 

PR2: 1S/1D 

PR3: 2S/2D 

PR1: 1S 

PR2: 1D 

PR3: 1S 

PR1: 1D 

PR2: 1S 

PR3: 1D 

PR1: 1S 

PR2: 1D 

PR3: 1D 

60 

Fish - 

Adult/Juveniles 


strata with 

shallow (seine) 

and deep (trawl) 

substrata 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

2 seines/ 

stratum 

1 trawl/ 

stratum 

72 

seines 

36 

trawls 

Ichthyoplankton 

and 


3 strata - channel 2 hauls/ 

stratum 

2 hauls/ 

Stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

72 

hauls 

Hydrology 


Maydell Drive (temperature, conductivity, and salinity @ surface and bottom). 

Entire reporting 

unit 

aerial 

1 

photo. 1 

shoreline 

1 

survey 1 

1 Conducted every five years.

Table 3.7 McKay Bay Strata 


MB1 0 1.1 

MB2 1.1 2.2 

Page 18 

Section 3.0 

April 2008 


Page 19 

Section 3.0 

April 2008 


Table 3.8 Sampling program summary for the McKay Bay reporting unit. 

Element 


Month 


Total 


Quality 

34 cells 3 4 3 4 3 3 10 10 10 3 4 3 60 

Benthos 34 cells 3 4 3 4 3 3 10 10 10 3 4 3 60 

Fish - 

Adult/Juveniles 

1 shallow 

(seine: 26 cells) 

and 1 deep 

(trawl: 18 cells) 

strata 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

1 seine in 

each of 

10 cells 

1 trawl in 

each of 

4 cells 

120 

seines 

48 

trawls 


and other 


6 cells – 

shallow 

2 strata - 

channel 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

Stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 haul/s 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

72 

hauls 

48 

hauls 

Hydrology 

22 nd Street Causeway (temperature, conductivity, and salinity @ surface and bottom). 


1 Conducted every five years. 

Entire reporting 

unit 

aerial 

1 

photo. 1 

shoreline 

1 

survey 1

Page 20 

Section 3.0 

April 2008 


Table 3.9 HBMP strata boundaries in the Alafia, Palm, and Hillsborough River reporting units. 

Stratum 

Down- 

Stream Limit 

(km) 

Upstream 

Limit (km) 

HBMP Station 

Number 

Lat_D Lat_M Long_D Long_M Description 

AR-1 0 AR100000 27 51.236 82 23.870 River mouth 

AR-1 2.33 AR102330 27 51.464 82 22.661 Near end of no-wake zone above marina 

AR-2 2.33 AR202331 27 51.464 82 22.661 Near end of no-wake zone above marina 

AR-2 4.67 AR204670 27 51.443 82 21.267 Approx. 200 m upstream of Alafia Marina 

AR-3 4.67 AR304671 27 51.443 82 21.266 Approx. 200 m upstream of Alafia Marina 

AR-3 7.00 AR307000 27 51.848 82 20.066 Near utility crossing between I75 & 301 

AR-4 7.00 AR407001 27 51.848 82 20.065 Near utility crossing between I75 & 301 

AR-4 9.33 AR409330 27 51.881 82 18.856 Near sharp bend in Alafia River at mouth of Rice Creek 

AR-5 9.33 AR509331 27 51.881 82 18.856 Near sharp bend in Alafia River at mouth of Rice Creek 

AR-5 11.67 AR511670 27 52.859 82 18.695 In straightaway a little over 1 km downstream from Buckhorn Springs 

AR-6 11.67 AR611671 27 52.859 82 18.694 In straightaway a little over 1 km downstream from Buckhorn Springs 

AR-6 14 AR614000 27 52.681 82 17.824 Near Kings Avenue Boat Ramp 

AR-7 14.00 AR714001 27 52.681 82 17.824 Near Kings Avenue Boat Ramp 

AR-7 18.50 AR718500 27 51.548 82 15.997 Approx. 200 m upstream of Bell Shoals Road 

PR-1 2.20 PR102200 27 56.548 82 24.677 River mouth where northern bank meets natural McKay Bay shoreline 

PR-1 3.95 PR103949 27 56.747 82 23.681 Approx. 200 m upstream of downstream railroad bridge 

PR-2 3.95 PR203950 27 56.747 82 23.681 Approx. 200 m upstream of downstream railroad bridge 

PR-2 5.69 PR205689 27 56.753 82 22.752 Near sharp bend in river at 68 St South - between Maydell Drive and trailer park 

PR-3 5.69 PR305690 27 56.753 82 22.751 Near sharp bend in river at 68 St South - between Maydell Drive and trailer park 

PR-3 7.44 PR307438 27 57.425 82 22.161 Just downstream of structure S-160 Dam 

HR-1 0 HR100001 27 56.495 82 27.518 Platt Street Bridge 

HR-1 2.55 HR102549 27 57.609 82 28.033 Just downstream of North Blvd. Bridge 

HR-2 2.55 HR202550 27 57.609 82 28.034 Just downstream of North Blvd. Bridge 

HR-2 5.09 HR205089 27 58.567 82 28.804 Just upstream of Duran Playground (between Columbus & MLK bridges) 

HR-3 5.09 HR305090 27 58.567 82 28.804 Just upstream of Duran Playground (between Columbus & MLK bridges) 

HR-3 7.64 HR307639 27 59.667 82 27.923 Approx. 150 m downstream of Hillsborough Ave. Bridge 

HR-4 7.64 HR407640 27 59.667 82 27.923 Approx. 150 m downstream of Hillsborough Ave. Bridge 

HR-4 10.18 HR410179 28 0.451 82 27.894 Near Epps Park (approx 250 m downstream of Sligh Ave. Bridge) 

HR-5 10.18 HR510180 28 0.451 82 27.894 Near Epps Park (approx 250 m downstream of Sligh Ave. Bridge) 

HR-5 12.73 HR512729 28 1.226 82 27.201 Just upstream of I275 Bridge at Sulphur Springs 

HR-6 12.73 HR612730 28 1.226 82 27.201 Just upstream of I275 Bridge at Sulphur Springs 

HR-6 16.20 HR616198 28 1.462 82 25.691 Just downstream of Hillsborough River Dam

Page 21 

Section 3.0 

April 2008 


Figure 3.2 

The Alafia River reporting unit. 

Page 22 

Section 3.0 

April 2008 


Figure 3.3. Illustration of the Alafia River HBMP strata and extended area for FIM monthly fish sampling. 

Page 23 

Section 3.0 

April 2008 


Figure 3.4 

The Palm River and McKay Bay 

reporting units. 

Page 24 

Section 3.0 

April 2008 


Figure 3.5 

McKay Bay reporting unit. 

Page 25 

Section 3.0 

October 2007 

Version 2.1 DRAFT

Figure 3.6 

Hillsborough Bay Reporting Unit 

Page 26 

Section 3.0 

October 2007 

Version 2.1 DRAFT

Appendix 3-A 

Detailed Maps of the HBMP Strata

Page1 

Section 4.0 

April 2008 



Data quality and effective data management are critical components of all monitoring 

programs. Data management activities including data collection, handling, evaluation, 

verification, validation, and reporting, are described in this section. Given the complexity 

of the HBMP, meeting data quality and management objectives will be crucial for 

effective and accurate data interpretation and report preparation. 

4.1 Data Quality Objectives 

An important goal of the HBMP is the development of data quality objectives (DQOs) 

that are integrated with environmental data collection activities. Data quality objectives 

are statements that describe in precise quantitative terms the level of uncertainty that can 

be associated with collected environmental data, and as such provide insight into the level 

of certainty that can be applied without compromising the intended use of the data. Use 

of data quality objectives also provides statistical criteria that can aid the design of 

sampling strategy elements, balancing costs, and/or resource constraints. 

Typically, DQOs are best developed by those identified as potential users of the data. In 

the absence of specific decision criteria, and the wide variety of potential uses to which 

the HMBP data may eventually be applied, the initial set of target DQOs are based on 

professional judgment, and are intended only to provide a starting point for a long-term, 

iterative DQO process. Consequently, these preliminary DQOs do not necessarily 

constitute definitive rules for accepting or rejecting results, but rather provide guidelines 

for continued improvement. Several iterations of the DQO process may be required as 

potential HBMP data users further define their specific needs. 

During the collection of data under the HBMP it will be extremely important to both 

control and determine measurement error to the greatest extent possible. Measurement 

quality objectives (MQOs) need to be established for each sampling field method and 

laboratory analysis procedure. MQOs essentially represent data quality objectives based 

on internal and external controls (variability) associated with each type of data 

measurement. As such, they can be used to establish criteria for data acceptability until 

reliable error bounds are established for each measured response variable (Table 4.1). As 

data are accumulated during the HBMP, error rates associated with each measurement 

will be established, and refinement of the initial target DQOs can be accomplished to 

determine the need for modifications to the sampling design and/or QA/QC Plan. 

Initial measurements of data quality objectives for each of the various parameters can be 

expressed in terms of accuracy, precision, and completeness goals. These preliminary 

MQOs are based on estimates of the most likely data quality achievable, based on: 

instrument manufacturer’s specifications; scientific experience; or data collected during 

other similar studies. In general, DQOs or MQOs are used to establish five aspects of

Page2 

Section 4.0 

April 2008 


data quality: representativeness; completeness; comparability; accuracy; and precision. 

Until actual study data are obtained, the MQOs used as quality control criteria for both 

field and laboratory measurements will be used to set the bounds of acceptable 

measurement error. 

4.1.1 Representativeness 

Representativeness is defined as “the degree to which the data accurately and precisely 

represent a characteristic of a population parameter, variation of a property, a process 

characteristic, or an operational condition” (Stanley and Verner 1985). The concept of 

representativeness within the context of the biological monitoring program refers to the 

ability of the sampling effort to accurately and precisely characterize the selected 

environmental indicators effectively both temporally and spatially. 

The design of the sampling program and the location of sampling sites provide the 

primary focus for defining the “representativeness” of population estimates for each 

reporting unit and strata. The HBMP employs a probability sampling approach that 

samples resources in proportion to their abundance and distribution to obtain unbiased 

estimates of resource characteristics and variability. The probability sampling approach 

applies systematic sampling to facilitate characterizations of spatial patterns and to 

encourage geographic coverage. 

Once unbiased quantitative information on the kinds, extent, condition and distribution of 

resources and associated estimates of uncertainty are known, a baseline of the status of 

existing conditions will be established. This baseline information will be used to develop 

criteria for identifying “representativeness,” the processes and magnitude of change 

associated with natural variation, and changes with time. 

The data quality attribute of “representativeness” applies not only to the overall sampling 

design, but also to individual measurements and samples obtained during all temporal 

and spatial aspects of the monitoring effort. Holding time requirements for different 

types of samples ensure that analytical results are representative of conditions at the time 

of sampling; these requirements are specified in the individual indicator sections of this 

document. In addition, the use of QA/QC samples, which are similar in composition to 

samples being measured, provides estimates of precision and bias that are representative 

of sample measurements. Therefore, as a general program objective, the types of QA 

samples (i.e., performance evaluation material) used to assess the quality of analytical 

data will be as representative as possible of the natural samples collected during the 

project with respect to both composition and concentration. 

4.1.2 Completeness 

Completeness is defined as “a measure of the amount of data collected from a 

measurement process compared to the amount that was expected to be obtained under the 

conditions of measurement” (Stanley and Verner 1985). All study elements within the

Page3 

Section 4.0 

April 2008 


HBMP have established completeness goals of 100% for each of the various indicators 

being measured. However, given the probability-based sampling design being employed, 

failure to achieve this goal will not preclude the within-year or between-year assessment 

of ecosystem condition. The major consequence of having less than 100% complete data 

from all expected stations is a relatively minor loss of statistical power in the areal 

estimate of condition, as depicted using Cumulative Distribution Functions (CDFs). The 

100% completeness goal is established in an attempt to derive the maximum statistical 

power from the present sampling design. Based on experience of other monitoring 

programs, failure to achieve this goal usually results from the field staff’s inability to 

sample at some stations due to logistical barriers such as insufficient depth, impenetrable 

substrate, or adverse weather conditions. In the limited number of instances where these 

conditions may be encountered, extensive efforts will be made to re-locate the station or 

re-sample the station at a later date. In this way, the field personnel must always strive to 

achieve the 100% completeness goal. In addition, established protocols for tracking 

samples during shipment and laboratory processing will be followed to minimize data 

loss following successful sample collection. 

4.1.3 Comparability 

Comparability is defined as “the confidence with which one data set can be compared to 

another” (Stanley and Verner 1985). Comparability of reporting units and calculations, 

database management processes, and interpretative procedures must be assured if the 

overall goals of the HBMP monitoring program are to be realized. A goal of the HBMP 

program is to generate extensive documentation to ensure that all future efforts can be 

made comparable. All field and laboratory methods are described in detail and available 

to all field personnel and analytical laboratory staff. In addition, the comparability of 

laboratory measurements will be established and monitored through duplicates and/or the 

use of field split and duplicate performance evaluation samples. The sampling design for 

each of the HBMP study elements has been made flexible enough to allow for analytical 

adjustments, if necessary, to ensure data comparability. 

4.1.4 Accuracy, Precision, and Total Error 

The term “accuracy”, is used synonymously with the term bias within this QA/QC plan, 

and is defined as the difference between a measured value and the true or expected value. 

Precision, by comparison, is defined as the degree of mutual agreement among individual 

repeated measurements. Collectively, accuracy and precision can provide an estimate of 

the total error or uncertainty associated with any individual measured value (Kirchner 

1983; Hunt and Wilson 1986; Taylor 1987). Measurement quality objectives for the 

various indicators are expressed separately as goals for both accuracy and precision. 

Accuracy and precision goals may not be definable for all parameters due to the nature of 

the measurement type. In order to evaluate the MQOs for accuracy and precision, 

various QA/QC samples will be collected and analyzed for most data collection activities.

Page4 

Section 4.0 

April 2008 


4.2 Routine Methods Used to Assess Precision and Accuracy 

4.2.1 Field Measurements 

1. Duplicate sets of field measurements are to be taken once per reporting unit per 

sampling day. These values (i.e., temperature, pH, DO, conductivity) will be 

documented as duplicates in the field notebooks and specifically used for 

determinations of instrument/sampling precision. 

2. Measurements of accuracy will be based on QA/QC checks of standards at the end of 

each sampling event. Standardized units of measure for critical indicators are shown 

in Table 4.2 

3. The precision of field measurements will be determined using the statistic “Relative 

Percent Difference” (RPD). 

RPD = {(|R1-R2|)/[(R1+R2)/2]} x 100 

Where: 

R1 = value of sample 

R2 = value of sample duplicate 

The RPD will be calculated for each duplicated pair of observations. The average or 

mean RPD is calculated by the formula: 

Mean RPD = Sum (RPDs) / n 

where n = number of duplicate pairs 

The standard deviation of the mean will be used to quantify precision measurements. 

The standard deviation of the mean is calculated by: 

s 2 RPD = [Sum(X i 2 ) - (Sum(X i ) 2 /n)] / (n-1) 

Where: 

X i = Each calculated RPD 

n = number of duplicate pairs 

Hence: 

s RPD = (S 2 RPD) ½ 

The s RPD will be calculated for the previous 20 duplicates for each in situ field 

parameter. Based on such duplicate pairs, control and warning limits for precision 

will be calculated. Standard protocol is to define the “Control Limit” as 2 times the 

s RPD , with the “Warning Limit” as 3 times the s RPD . Using these procedures, control 

and warning charts can be generated to flag observations that exceed either Warning 

and/or Control Limits.

Page5 

Section 4.0 

April 2008 


4.2.2 Laboratory Water Quality Parameters 

1. The procedures that will be used to determine water quality precision and accuracy 

targets for the HBMP are outlined below and listed in detail in the Quality Manual for 

Southern Analytical Laboratories, Inc. (July, 2001). 

2. Duplicate water quality grab samples will be taken at a rate of 5% of samples with a 

minimum of one duplicate per sampling day. Estimates of precision will be based on 

duplicate analyses of each parameter. Relative percent differences will be calculated 

using the statistic outlined above. 

3. Estimates of accuracy will be determined using the calculation: 

[(A-B)/C] X 100 = % Recovery 

Where: 

A = concentration of spiked sample 

B = concentration of sample 

C = concentration of spike added to sample 

Control charts can then be produced on % recovery data to show the upper and lower 

warning and control limits. Calculations of warning and control limits for accuracy 

are similar to those for precision with mean % recovery and standard deviation of the 

mean calculated by standard statistical formulas used above. 

4. Precision and accuracy targets will be obtained using matrix spikes, sample 

duplicates, duplicate matrix spikes, and QC check samples. These procedures are 

conducted on various concentration levels defined as: 

• Low Level: Concentrations between the method Minimum Detection Limit 

and 5 times the MDL 

• Mid Level: Concentrations between the method Minimum Detection Limit 

and the upper end of the linear response 

• High Level: Concentration at the upper end of the linear range. 

4.3 Method Detection Limits 

1. Method Detection Limit (MDL) - Reagent blank water is used to prepare a laboratory 

standard in a concentration range 1 to 5 times the estimated MDL of the specific 

compound of interest (Table 4.3). If the estimated MDL is found to be correct then 

seven aliquots of the standard are analyzed using the complete analytical method.

Page6 

Section 4.0 

April 2008 


The following calculations are then used to determine the MDL, and the Practical 

Quantification Limit (PQL). 

As per EPA procedures in 40 CFR 136 Appendix B, the MDL and PQL are determined 

by the following: 

where: SD = standard deviation of 7 replicate measurements 

MDL = 3.14 times the SD 

PQL = 12 times the SD 

2. MDLs are required to be recalculated when there are substantial changes in either the 

instrumentation or technique used. 

4.4 Data Reduction and Validation 

Figure 4.1 presents a simplified overview of the data management processes that will be 

utilized during the HBMP. Figure 4.2 further details the major components which will be 

included within specific sections of the overall HBMP database. 

The HBMP database will be implemented using Microsoft Access®, a fully relational 

database that is readily available to many users. Export of data from Access to other 

software applications such as Excel (or other spreadsheets), SAS, and HTML for Internet 

applications will be facilitated through the use of this standardized data application. The 

use of a relational database will ensure that many different data types can be effectively 

linked using common variable names and values. 

At the beginning of each year, the process will be initiated by choosing sampling sites 

and sampling dates for the upcoming year. These sampling sites will be made available 

to all pertinent parties, including: the Project Manager; QA/QC Officer; the Field Control 

Officers for each of the sampling program elements; the PBS&J Sample Custody Officer; 

and the Database Manager. 

4.4.1 HBMP Data Sources 

The HBMP is comprised of a number of separate and unique sampling efforts being 

conducted by each of the various members of the Project Team. Certain members of the 

Project Team have ongoing, standardized data handling processes and verification and 

data storage methodologies (Southern Analytical Laboratories, Florida Fish and Wildlife 

Research Institute, Terra Environmental, and the University of South Florida). For this 

reason, the initial: 1) logging of field and laboratory records; 2) data checking steps; 3) 

data review; and 4) internal QA/QC documentation, will follow the existing established 

protocols and will address the particularly unique criteria of each of these study elements 

(see appropriate sections of this document). Raw hard copy data will be permanently

Page7 

Section 4.0 

April 2008 


maintained, and after being visually form checked for errors, will be entered into 

electronic format using appropriate protocols and multiple levels of checks to assure that 

all data quality goals are satisfied. 

Data from each HBMP study element, as well as other outside sources (SWFWMD, 

USGS, EPCHC, etc.), will contain a standardized, unique header record containing all of 

the information necessary to subsequently link each of the types of data, spatially and 

temporally, within the final validated Access database (see Figure 4.2). Such header 

information will include, but not be limited to: sampling group; date; time; reporting unit; 

substratum; and station location (such as river kilometer). Typically, the data for a given 

HBMP program element will be aggregated by year and month within the data set. In 

addition, other data types will be formatted for inclusion into the HBMP Access database 

(such as USGS flow data and SWFWMD rainfall data). 

The “condition field” of all HBMP data which has been entered, checked through at least 

two steps for errors, and received the application of any necessary reduction 

methodologies (dilutions, calculations of species numbers, etc.) will remain designated as 

“raw” until undergoing further steps in the data validation protocol (see Figure 4.1). 

Following the development, logging, and checking of all “raw” data, error and range 

checking procedures for data verification purposes will be performed. During the 

verification process, specific data conditions will be noted using appropriate “data 

qualifier” designations. Standard DEP/EPA data qualifier codes will be used, as needed, 

in the appropriately identified database field (Appendix 4-A). Only after all such 

appropriate levels of data validation have been completed, will the “condition field” 

within each specific data record be changed from “raw” to “verified.” Only the database 

manager and his/her designees will have read/write abilities at this level. All other 

database users will be provided with read-only access to data that has been listed as 

“raw” and is in the verification process. Any errors and/or other changes of data made 

between the “raw” and “verified” status must be fully explained in the appropriate “note 

field,” and a permanent written record of any such changes will be maintained by the 

QA/QC Officer. 

Cross comparison of “verified” data from the different HBMP elements will allow 

further, subsequent, “validation” of each specific data entry. Final review of the data in 

this Verified Data Set will also include any other cross comparisons, for example, 

between field and laboratory data, or between taxonomic experts. Only after final 

QA/QC review of each specific subset of data (monthly for water quality, quarterly for 

most other project study elements) will the QA/QC Officer update the “verified” status of 

the data to “validated.” Only the QA/QC Officer will have secure password access to the 

data at this level. Only under unusual circumstances will data not designated as 

“validated” be made available for general dissemination. The QA/QC Officer, after final 

review with the appropriate specific project leaders, will be responsible for applying the 

final “data qualifier” and “data flags” within the database. These will be used to inform 

the end-users of the data of any problems that may have been found to exist in the data

Page8 

Section 4.0 

April 2008 


that could not be reconciled during the verification and validation checks of the data. In 

addition to the standard “data qualifiers,” three levels of “data flags” will be used in a 

separate data field. These include: 

• accepted - this type of designation will be used to designate data that has successfully 

met all established QA/QC protocols, procedures and standards. End users should 

encounter few, if any, difficulties utilizing data with this designation. 

• rejected - this designation will be used to indicate data which did not meet a 

significant element of the established QA/QC protocols, procedures or standards. 

Specific notes within the database will explain all such designations (i.e., chlorophyll 

a filters were not appropriately frozen and arrived at the Laboratory in an 

unacceptable condition). End users should never use any data with such a 

designation. 

• provisional - such data was collected, passed almost all of the required QA/QC 

protocols, but failed some minor QA/QC procedure or standard. Such problems will 

be noted in the database (i.e., the water chemistry sample for color was analyzed 1 

hour outside its required hold time). Even though such values have a high 

probability of being representative of actual conditions, such data should probably 

be used with caution. 

4.4.2 Outside Data Sources 

Some of the data that will be utilized by the HBMP will come from other outside sources. 

Examples include daily river flows, withdrawal rates, rainfall, other meteorological data, 

aerial photos, and GIS data that will be obtained on a relatively routine basis. In addition, 

the majority of the data to be used in assessing the status of Hillsborough Bay will come 

from sources, such as EPCHC, FWRI, and SWFWMD. The schedule for downloading 

data from other sources will clearly depend upon when these data are available. 

Data transfer formats will be defined in consultation with the data providers. Care will be 

taken to ensure that all data received will be appropriately documented, to the greatest 

extent possible. This documentation will include definitions of variables and variable 

codes, units of measure, methods, and spatial definition of samples (e.g., latitude and 

longitude of sampling point). When possible, cross-comparisons of these data and the 

data collected by the HBMP will be made as an additional quality assurance check. 

Historical data from other sources will also be used in the analysis of the HBMP data. 

The HBMP data management process will support data retrieval and documentation from 

all of these other sources to ensure the data from these sources are correctly linked with 

the primary HBMP data sources.

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4.4.3 Data Dictionary 

A critical step in overall data management is the development and maintenance of an 

accurate data dictionary, which defines specific characteristics of all variables within the 

HBMP database. Specific details regarding the HBMP Data Dictionary will be 

maintained within the developed Access database. The structure of the HBMP Data 

Dictionary (Appendix 4-B depicts its initial structure) will include the following types of 

information: 

• Field - the name of the variable whose data are reported in this field. 

• Description - a definition of the variable. 

• Units - specific units of measure used. 

• MDL - minimum detection limit, all water chemistry data values reported as detection 

limit will be entered into the database as the actual detection limit. For example, the 

laboratory reports the chlorophyll a concentration for a particular sample as < 1.0 

mg/l. Since the field is designated as numeric, it will be identified as below detection 

limit by entering the value 1.0, and an appropriate data qualifier. This will allow the 

end user to easily identify all detection limit values in the database and use whatever 

convention they choose for analyzing such data. This procedure will also clearly 

indicate any potential changes in detection limits during the course of the HBMP. 

• Format - digital or alphanumeric format. 

• Typical values - range of typical values (data entry criteria can be established to 

automatically query entries outside normal ranges). 

• Legal values - may be general or specific (i.e. only positive numeric values). 

• Missing values - defines how missing values are coded or in some cases no missing 

value may be allowable (e.g., date of sample will always have some value before data 

are accepted into the database). 

• Condition - status during QA/QC review regarding level of data entry, checking, 

verification and validation. 

• Data Qualifiers - DEP/EPA standardized, designated codes that address specific 

QA/QC limitations to field and laboratory data. 

• Data Flags - specific designation of all values within the final “validated” data set. 

• Notes - a placeholder for any notes that the dictionary user may find useful.

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• The HBMP database dictionary will be updated every year to ensure that all database 

users are kept fully abreast of the database contents and their use. 

4.5 Data Methodologies 

4.5.1 Field Data 

All collections of in situ field data are to be under the direct control of the Field QA/QC 

Officer. A key responsibility of the field staff will be to continually monitor and check 

the status and performance of all equipment. Calibrations of all field instruments are to 

be performed prior to the beginning of each sampling event (see relevant sections of the 

QA/QC Plan for each HBMP element). 

All field in situ measurements of depth, temperature, pH, specific conductance, and 

dissolved oxygen measurements are by their very nature non-repeatable. As such, 

corrective actions for non-repeatable measurements are severely limited to: 

• detecting and discarding invalid data at the time of collection; and 

• eliminating a potential situation which could result in creating invalid data. 

Detecting and checking potentially questionable or invalid data in the field at the time of 

measurement provides the highest opportunity to correct problems without losing data. 

The Field Control Officer will be responsible for making all on-site decisions about the 

measurement data. Outliers or otherwise unusual data are only to be accepted after the 

instruments have been checked for malfunction, the calibration has been verified, and the 

reading verified by re-submerging the probe. For this reason, it is extremely important 

that the field staff be familiar with the project, the instruments and expected range of data 

values, thus allowing them to effectively screen nearly all abnormal values and/or 

questionable field water quality values. Instrument “drift” will be monitored by 

comparison of pre- and post-field calibrations for each multi-parameter sonde. In 

addition, field and project management staff will continually evaluate performance 

aspects to determine if changes in calibration, sampling and measurement procedure 

could be utilized to further reduce the risk of collecting invalid data. 

Outliers will be accepted only after the instrument has been checked for malfunction, the 

calibration verified, and the water column profile run in duplicate. Corrective action for 

field measurements may also be initiated using known standards for pH and conductivity 

and field duplicates for all field parameters. Should the difference between duplicates or 

the value of known samples be greater than 5%, then corrective action will be initiated. 

If instrument malfunction occurs in the field, options for corrective action from most 

desirable to least include: 

1. Use a backup instrument if available. Should the primary field instrument fail, a 

calibrated backup instrument should be substituted if at all possible.

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2. Recalibrate the primary instrument and frequently check to assure that the problem 

has been corrected. 

3. Postpone the sampling event and return with a working multi-parameter sonde at 

another time. 

All pertinent field notes and in situ data from the field instruments (multi-parameter 

sonde, Secchi disk, etc.) are to be directly entered from the field notebooks into the 

PBS&J HBMP Access database. 

The Field Control Officer and technical staff will be responsible for correctly entering all 

field notes and in situ readings into the field notebooks (see Section 5 of the QA/QC 

Plan). Prior to returning from the field, the Field Control Officer will be responsible for 

checking all data entered into the field notebooks. The Field Control Officer will also be 

responsible for seeing that all responsible field staff review the data and obtain signatures 

certifying to the completeness and validity of all data entered into the notebooks. Upon 

returning from the field, the Field Control Officer will be responsible for storing field 

notebooks in a secure location. The Field Control Officer is responsible for ensuring 

copies of newly collected data in field logbooks are made at least once per week. This set 

of field notes will be placed sequentially in a three ring binder as a secondary, 

permanently maintained set of backup field notes. 

After the completion of each month’s sampling (or sooner if a notebook is full), the Field 

Control Officer will give the original field notebook to the data entry staff for entry of 

field parameters into the Access Data Management System. After the field 

measurements have been entered into the database, the data entry staff will again check 

all entries (and correct, if necessary). After all values have been proofed by the data 

entry staff, the original field notebook will be stored in a secured location. All data 

entered for the current month will be automatically marked as “Provisional” at this point. 

In addition, it will be the responsibility of the QA/QC officer to check the reasonableness 

of all data based on both historic ranges and values collected from upstream and 

downstream sampling locations. Based on these reviews, the Project QA/QC Officer will 

be responsible for changing the status codes in the database from “Provisional” to either 

“Accepted” or “Rejected.” The values of any rejected data will be recorded in the 

comment field. The Project QA/QC Officer will be responsible for informing the Project 

Manager as to the status of all data within the project database. 

4.5.2 Laboratory Data 

Field staff will collect sub-surface samples at each of the sampling locations for the 

analysis of selected water quality parameters. Each sample container is to be identified 

through the use of printed labels. Information required on the labels will include: 

• date;

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• time of sample collection; 

• initials of the field personnel who collected the sample; 

• project identification; 

• sample location identification; and 

• list of water quality parameters to be analyzed. 

As each container is filled, sampling data are also logged into a field notebook. Specific 

procedures for the handling, tracking and shipping of water chemistry samples to the 

Laboratory are described in detail in Section 5 of this QA/QC Plan. When each set of 

samples reaches the laboratory, the Sample Custody Officer will check all accompanying 

sample custody/tracking forms to assure completeness. All water quality samples will be 

analyzed using the units, methods and detection limits presented in Table 4.3. Standard 

laboratory protocols and sample tracking procedures are outlined in Section 5 and 

specified in detail in the Quality Manual for Southern Analytical Laboratories, Inc. (July, 

2001). All monthly water chemistry data will be transmitted, as soon as it is available, 

from the Laboratory to the Project Manager and Project QA/QC Officer. It will be the 

responsibility of the QA/QC officer to check the reasonableness of all data based on: 

• laboratory QA/QC documentation, including duplicates, spike additions, and 

compliance with control limits; 

• historic ranges; and 

• similar data collected during the same day from upstream and downstream locations. 

Based on these reviews, the Project QA/QC Officer will be responsible for ensuring data 

entry staff import electronic files provided by the laboratory into the project database, 

assuring that all parameters were imported correctly, and assigning an appropriate status 

code for each parameter in the database (“Provisional,” “Accepted,” “Rejected”). 

4.6 Data Storage 

4.6.1 Field Data 

All original field data will be permanently maintained in the field notebooks. All original 

field notebooks will be under the control of the Project Manager while not actually out in 

the field. The notebooks are to be kept in a secure location. Two sets of copies of the 

field notes from each sampling event will be made and maintained separately in the 

PBS&J files/archives and by the Project Manager (may be maintained electronically as 

scanned images). 

4.6.2 Water Chemistry Data 

All original chemistry results, standard curves, duplicate and spike addition results, etc. 

will be permanently maintained within chemistry bench laboratory notebooks as 

prescribed in the Quality Manual for Southern Analytical Laboratories, Inc. (July, 2001).

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All final chemistry results will be entered into the Laboratory’s computer database. 

System-wide archive copies of all data contained within this database are backed-up on a 

minimum of a weekly basis. 

4.6.3 HBMP Database 

All data will be entered into the HBMP Access Database (see above). Daily and weekly 

backups are made of all data within the PBS&J system as part of normal network system 

protocols. In addition, both the Project Manager and Project QA/QC Officer will 

maintain backup copies of all HBMP data on compact disks on an ongoing basis during 

the course of the project. Yearly summary data sets of all data will be submitted to 

Tampa Bay Water in conjunction with the annual reporting process. These procedures 

will be utilized to assure that all historical records, both written and electronic, are to be 

maintained in perpetuity. 

4.7 Electronic Data Records 

PBS&J uses a Local Area Network system comprised of IBM Compatible microcomputers 

and peripherals. All field data, analytical results, and QA/QC data are to be 

stored within the Access database on the fixed disk of the file server. Daily and weekly 

database backups are performed and maintained. 

Data integrity and strict computer system security will be maintained at all times. Each 

computer in PBS&J’s local network system is password protected, limiting access to the 

computer system to only authorized employees. Within the database, a second level of 

security is maintained through a password system. Project staff, other than the QA/QC 

Officer and lead database personnel, may browse data or print reports with a read-only 

access code. Password coding does not permit the changing or alteration of any data 

entered in the database, without proper authorization. 

Copies of all database application programs are reprinted and maintained each time there 

is a modification. Software documentation sections consist of: 

• a complete list of relational data storage tables in the database; 

• a list of data column names and descriptions for each column in a data storage table; 

• a hierarchically arranged printout of the database menu system; 

• a printout of each application program with internal documentation describing the 

function and variable definitions for subroutines within the application program; 

• a hierarchically arranged printout of all data entry screens;

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• a printout of any calculation routines; and 

• examples of all reports and any forms generated by the computer system. As new 

routines are added or applications reprogrammed, the appropriate pages in the 

documentation are either inserted or substituted as needed. 

4.8 Performance and System Audits 

4.8.1 Internal Field System Audits 

Audits will be performed on a regular basis to assure compliance of established practices 

consistent with the QA/QC Plan. At that time, problems and/or deficiencies will be noted 

and improvements addressed. The basis for review will be the SOPs and protocols 

specified in this Project Specific QA/QC Plan. Internal review of field activities will be 

divided into the following major categories: 

• Field instrument calibrations and QA/QC checks; 

• Field instrument and equipment maintenance; 

• Methods utilized to locate designated sampling sites; 

• In situ sampling protocols, measurements (i.e. multi-parameter sonde readings) 

including corrective actions; 

• Sample collection methods including preservation, filtration, handling and 

transportation; 

• Field documentation; and 

• Field and sample chain of custody. 

Upon completion of each QA/QC review by the Project QA/QC Officer, the Project 

Manager will be provided with a brief written summary of all observed deficiencies. The 

Project Manager will then implement and document all actions taken to correct any 

observed deficiencies. At least annually, all technical staff will meet to review and 

discuss all procedures and protocols in detail. However, any significant changes in 

personnel or major version changes to the protocols will require additional formal 

reviews.

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4.8.2 External Field System Audits 

All work performed under the HBMP may be subject to on-site systems audits conducted 

by outside agency staff (potentially including DEP, SWFWMD, and EPCHC). All 

members of the project team will accommodate such on-site systems audits with notice. 

4.8.3 Internal Laboratory System Audits 

Southern Analytical participates in the Southwest Florida Regional Ambient Monitoring 

Program (RAMP). This program consists of a group of professionals from around the 

region involved in water quality sampling and analysis of surface waters. The objectives 

of the group are to produce accurate and compatible water quality data among the groups 

monitoring water quality in southwest Florida and to reduce redundancy in sampling 

sites. PBS&J receives quarterly reports resulting from RAMP meetings that illustrate 

how lab analyses conducted at Southern Analytical compare with other laboratories in 

southwest Florida. The HBMP Project QA/QC Officer will meet with the Laboratory 

Quality Assurance Officer as needed to review any issues that may arise as a result of 

RAMP. 

Additionally, the Project QA/QC officer may conduct an audit to evaluate those 

components of the laboratory’s Comprehensive Quality Assurance Manual pertinent to 

the HBMP. At that time, any problems or potential deficiencies will be documented and 

corrective actions addressed. Items for the Laboratory System Audits can be divided 

into the following categories: 

• Sample container preparation; 

• Laboratory glassware cleaning and storage; 

• Instrument calibrations and QA/QC checks; 

• Sample check-in, distribution and chain of custody; 

• Data reduction, validation and reporting; 

• Laboratory notebook documentation; and 

• Database management 

4.8.4 External Laboratory Systems and Performance Audits 

The Laboratory receives on-site evaluations and/or inspections by the Department of 

Health on an annual basis. This is a complete system audit. At the completion of the 

inspection any deficiencies discovered are noted in writing and corrections documented.

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Internal performance audits are coordinated by the Laboratory Quality Control Officer to 

evaluate accuracy of particular chemical analyses. This is to be done by introducing 

blind samples of known standards (unknown to the analyst) into the normal flow of 

chemistry analysis and checked for accuracy. External performance audits comprise 

participation in the EPA Water Supply and Water Pollution evaluation performance 

studies which are conducted on a semiannual basis.

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Table 4.1 Measurement Quality Objectives for Indicators 

Indicator/Data Type Accuracy (Bias) Precision Completeness 

Water Quality: 

Goal Goal Goal 

Physical In Situ 5 % 10 % 100 % 

Inorganics 5 % 10 % 100 % 

Species composition: 

Sorting 10 % NA 100 % 

Counting 10 % NA 100 % 

Taxonomy 10 % NA 100 % 

* Accuracy (bias) goals are expressed either as absolute difference (∀ value) or percent deviation from 

the "true" value. Precision goals are expressed as relative percent difference (RPD) or relative standard 

deviation (RSD) between two or more replicate measurements. Completeness goal is the percentage of 

expected results that are obtained successfully.

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Table 4.2 Standardized Units of Measure 

Critical Indicator 

Units 

Stream Flow 

cubic feet/second 

Water Level 

m 

Salinity 

PSU 

Specific Conductance 

umhos/cm 

Temperature 

pH 


Sample Depth 

Secchi Disk Depth 

Light Transmission 

Color 


Orthophosphate Phosphorus 

Total Phosphorus 

Ammonia Nitrogen 

Ammonium NH4 

Nitrate-Nitrite Nitrogen 

Total Kjeldahl Nitrogen 

Total Nitrogen 

Chlorophyll a 

o C 

standard pH units 

mg/L 

m 

m 

k d 

PCU 

mg/L 

mg/L - P 

mg/L - P 

mg/L - N 

mg/L - N 

mg/L - N 

mg/L - N 

mg/L - N 

ug/L

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Table 4.3 Field and Laboratory MDLs for 

HBMP Water Quality Parameters 

Indicator Units of Measure Method MDL 

Field Measurements – PBS&J, USF and FWRI 

Water Temperature DEP FT1400 deg C 0.01 

pH DEP FT1100 Standard Units 0.01 

Conductivity DEP FT1300 umhos/cm 0.1 

Salinity DEP FT1300 ppt 0.1 

Laboratory Methods – Southern Analytical Laboratory 

Color SM 2120 B PCU 5 

Total Suspended Solids EPA 160.2 mg/L 2 

Orthophosphate Phosphorus EPA 365.2 mg/L - P 0.01 

Total Phosphorus EPA 365.2 mg/L - P 0.03 

Ammonia Nitrogen EPA 350.1 mg/L - N 0.01 

Ammonium NH4 Calculation mg/L - N 0.01 

Nitrate-Nitrite Nitrogen EPA 353.2 mg/L - N 0.01 

Total Kjeldahl Nitrogen EPA 351.2 mg/L - N 0.05 

Total Nitrogen Calculation mg/L - N 0.05 

Chlorophyll a SM 10200H ug/L 2

Figure 4.1 Overview of the HBMP Data Management Process 

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Figure 4.2 Overview of the Major Contents of the HBMP Database 

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Appendix 4-A 

Applicable DEP/EPA Data Qualifier Codes 

Symbol 

Meaning 

A 

D 

E 

F 

J 

Value reported is the mean (average) of two or more determinations. This code 

shall be used if the results of two or more discrete and separate samples are 

averaged. These samples shall have been processed and analyzed (e.g. laboratory 

replicate samples, field duplicates, etc.) independently. Do not use this code if the 

data are the result of replicate analysis on the same sample aliquot, extract or 

digestate. Under most conditions, replicate values shall be reported as individual 

analyses. 

Measurement was made in the field (i.e. in situ). This applies to any value (ex. pH, 

specific conductance, etc.) that was obtained under field conditions using approved 

analytical methods. Note: When data is to be entered into STORET, and 

parameter code specifies a field measurement (e.g. "Field pH"), this code is not 

required. 

Indicates that extra samples were taken at composite stations. 

When reporting species: F indicates the female sex 

Estimated value; value not accurate. This code shall be used in the following 

instances: 

1. surrogate recovery limits have been exceeded; 

2. no known quality control criteria exists for the component; 

3. the reported value failed to meet the established quality control criteria for 

either precision or accuracy; 

4. the sample matrix interfered with the ability to make any accurate 

determination; 

5. if the data is questionable because of improper laboratory or field protocols 

(e.g. composite sample was collected instead of a grab sample). 

Note a "J" value shall be accompanied by justification for its use. 

A "J" value shall not be used if another code applies (e.g., K, L, M, T, V, Y, 

I) 

K 

Off-scale low. Actual value is known to be less that the value given. This code 

shall be used if: 

1. The value is less than the lowest calibration standard and the calibration 

curve is known to be non-linear; or

2. The value is known to be less than the reported value based on sample size, 

dilution or some other variable. 

L 

M 

M 

O 

Q 

R 

T 

U 

V 

Y 

This code shall not be used to report values that are less than the laboratory 

practical quantitation limit or laboratory method detection limit. 

Off-scale high. Actual value is known to be greater than value given. To be used 

when the concentration of the analyte is above the acceptable level for quantitation 

(exceeds the linear range or highest calibration standard) and the calibration curve 

is known to exhibit a negative deflection. 

When reporting chemical analyses: presence of material is verified but not 

quantified; the actual value is less than the value given. The reported value shall 

be the laboratory practical quantitation limit. This code shall be used if the level is 

too low to permit accurate quantification, but the estimated concentration is 

greater than the method detection limit. If the value is less than the method 

detection limit use "T" below. 

When reporting Species: indicates male sex. 

Sampled, but analysis lost or not performed. Note: If reporting data to the U.S. 

Environmental Protection Agency Water Quality Storage and Retrieval (STORET) 

database, a numerical value must be entered. Such values are not meaningful and 

shall not be used. 

Sample held beyond the accepted holding time. This code shall be used if the 

value is derived from a sample that was prepared or analyzed after the approved 

holding time restrictions for sample preparation or analysis. The number of days 

beyond the designated holding time will be report following the letter designation. 

Significant rain in the past 48 hours. This code shall be used where the rainfall 

might contribute to a lower than normal value. 

Value reported is less that the laboratory method detection limit. The value is 

reported for informational purposes, only and shall not be used in statistical 

analysis. 

Indicates that the compound was analyzed for but not detected. This shall be used 

to indicate that the specified component was not detected. The value associated 

with the qualifier shall be the laboratory method detection limit. Less than the 

method detection limit values shall not be reported (see "T" above). 

Indicates that analyte was detected in both the sample and the associated method 

blank. Note: The value in the blank shall not be subtracted from associated 

samples. 

The laboratory analysis was from an unpreserved or improperly preserved sample. 

The data may not be accurate.

I 

The reported value is between the laboratory method detection limit and the 

laboratory practical quantitation limit. 

! Data deviates from historically established concentration ranges. 

? Data is rejected and should not be used. Some of all of the quality control data for 

the analyte were outside criteria, and the presence or absence of the analyte cannot 

be determined from the data. 

Χ 

Not analyzed due to interference. Note: If reporting data to the U.S. 

Environmental Protection Agency Water Quality Storage and Retrieval (STORET) 

date base, a numerical value must be entered. Such values are not meaningful and 

shall not be used. 

Note: 

If more than one code applies, all will be noted within the HBMP database 

in the QA/QC notes. However, in the “data qualifier field”, as if the data 

were to be entered in STORET, only one code shall be reported. The code 

shall be selected based on the following hierarchy: 

1) ? 

2) *, O 

3) Y 

4) V 

5) H 

6) B, K, L, M, I, T, Z, U, N, Q 

7) A, F 

8) J

Appendix 4-B Data Dictionaries for each of the HBMP Study Components 

Data Dictionary - Primary Linkages (Headers) 

Variable 

Name 

Linkage Format Acceptable Values Additional Explanation 

group Project Team alpha PBSJ, UF, USF, FRMI, USGS, EPC, DISTRICT, etc. Other codes will be added fro additional data sources as they become available 

year Year numeric 00 and ongoing Current contract extends from 00 through 05 

month Month numeric 01 through 12 January through December 

unit Reporting Unit alpha HR, AF, MB, PR HR = Hillsborough River AF = Alafia River 

MB = McKay Bay PR = Palm River (Bypass) 

strat Stratum numeric 0 through 8 Number of strata varies with Reporting Unit 

site Sampling Location alpha - numeric two letters followed by 5 digits HR101480 - means: Hillsborough River Reporting unit; stratum #1; river meters 01480 (from mouth of river)

Data Dictionary - Field Notes 

Variable 

Name 

Format Acceptable Values Additional Explanation 

lat Latitude numeric two digits, space, two digits, three decimal places degrees, minutes, decimal seconds (27 52.758) 

long Longitude numeric two digits, space, two digits, three decimal places degrees, minutes, decimal seconds (82 18.754) 

Tide Tidal Stage numeric 1 through 8 1 =high slack 2 = high outgoing 3= mid outgoing 

4 = low outgoing 5 = low slack 6 = low incoming 

7 = mid incoming 8 =high incoming 

Temp Air Temperature numeric 0 to 35 Approximate air temperature at the time of sampling 

Wind Wind alpha - numeric 0 to 50 mph Direction (compass heading) and Speed (approximate mph) 

Cloud Percent Cloud Coverage numeric 0 to 100 % by units of 5 Approximate level of cloud coverage within general area 

note Field Observations text Any unusual observations or other notes made in the field

Data Dictionary - Hydrology & Water Quality Parameters 

Variable 

Name 

Critical Indicator Units Format Expected 

Range 

Legal 

Values 

MDL 

Missing Data 

(Values) 

rain Rainfall inches/day Numeric 0 10 0 < & < 25 0.01 -9999 raw, verified 

or validated 

flow Stream Flow cubic feet/second numeric 0 10000 0 < & < 25000 1 -9999 raw, verified 

or validated 

gauge height Water Level m numeric -1 2 -3 < &

Variable 

Name 

Critical Indicator Units Format Expected 

Range 

Legal 

Values 

MDL 

Missing Data 

(Values) 

TOC Total Organic Carbon mg/L numeric 1 100 -1< &

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This section describes the protocols, methods, and procedures that comprise the Water 

Quality and Hydrology elements of the HBMP. It is imperative that the field and water 

quality sampling methods and procedures used by HBMP personnel correspond to those 

contained in this portion of the QA/QC Plan. These standardized sampling procedures 

and techniques will ensure data that can then be combined or compared as appropriate. 

Figure 5.1 provides an overview of the organizational management of this study element, 

while Table 5.1 summarizes both the number and frequency of samples collected yearly 

in each of the sampling strata. 

Members of the teams conducting these elements of the HBMP must read, understand 

and follow all steps in each procedure outlined below. If questions or suggestions arise, 

the Project Manager must be consulted for clarification before any further actions can be 

taken. Under no circumstance should a procedure or steps within a procedure be omitted 

or modified. 

5.1 Objectives 

The objectives of the hydrologic monitoring elements of the HBMP include: 

• Estimating daily freshwater inflows for each of three reporting units (lower Alafia, 

lower Hillsborough and TBC/Palm Rivers); 

• Estimating daily total freshwater withdrawals from each of these reporting units; and 

• Measuring water levels for each of these reporting units. 

A significant portion of these data are or will be collected by other governmental 

agencies. To the greatest extent possible, PBS&J will work to see that the methods and 

procedures used to collect hydrologic parameters as part of the HBMP are comparable 

with similar data being gathered by other agencies. 

The objectives associated with the ambient water quality monitoring elements of the 

HBMP are to: 

• Estimate the spatial distribution and variance of both in situ water column profiles 

and surface water quality indicators within each of the reporting units; and 

• Develop estimates of variation of these water quality indicators on a temporal basis 

for each of the reporting units.

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5.2 Hydrology/Water Quality Indicators 

A thorough and comprehensive discussion of desirable and critical indicators is presented 

in Section 3 of this document, as well as in the HBMP design document Tampa Bypass 

Canal/Alafia River Water Supply Projects Hydrobiological Monitoring Program 

(PBS&J, 2000). Table 5.2 provides a summary of the selected hydrology/water quality 

critical indicators, their primary sources of variability, and the potential effects of the 

permitted surface water withdrawals for each. 

Due to both the natural and anthropogenic differences among the three reporting units 

(e.g., hydrologic and ecologic characteristics, the degree of previous impacts, identified 

data gaps, etc.) not all of the selected critical indicators were determined to be applicable 

to all waterbodies. Therefore, a specific suite of critical hydrology/water quality 

indicators (Table 5.3) was selected for implementation in each respective reporting unit. 

5.3 Sample Collection Techniques 

Field sampling will normally be conducted by at least two PBS&J staff whenever 

possible with the senior member having extensive experience in field data collection. 

Field crews will be trained in all sampling procedures and will have copies of all the 

pertinent chapters of the HBMP Project Specific QA/QC Document to consult in the 

field. 

5.3.1 Pre-Mobilization 

Whenever possible all initial field mobilization and preparation should be completed by 

the end of the day prior to each sampling event, in order to reduce potential mistakes. 

Dissolved oxygen membranes should be checked at least 24 hours prior to calibration in 

order to allow sufficient time for stabilization should the membrane need to be replaced. 

1. Prior to leaving for each field sampling event, the field staff will check all Sample 

Custody Forms and the Project Logs and Checklists to make sure that they have: 

• properly cleaned and labeled sampling containers for each parameter group to be 

collected from each of the designated sampling locations. Pre-cleaned, unpreserved 

sampling containers for each chemistry parameter group must be 

arranged, checked and bagged (by sampling unit and location) for transport. 

Containers are to be checked against project pre-logs for correctness. Each 

container should be marked with the appropriate project number, ID number, 

station number, sample number, and chemistry parameters or groups to be 

collected. 

• Sample bottles for both field duplicates and field blanks.

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• At least two complete sets of duplicate sample containers which could be used in 

the event that any of the normal samples containers are lost or contaminated 

during the field collection. 

• All appropriate Sample Custody and Tracking Forms. 

2. The field staff must check to see that all needed gear is functional (Project Checklist), 

and that all water sampling devices have been pre-cleaned as necessary. If field 

cleaning of equipment will be required, Liquinox, analyte-free water, washing and 

waste collection containers must be taken. If possible, backup sampling gear should 

be included to preclude possible problems in the field 

3. Check measurements of all depth marks on calibrated lines (multi-parameter sondes 

and Secchi Disk) and repair any damaged marks. (All lines will be recalibrated after 

any repairs, such as after sending sonde cables back for replacement of connectors, or 

other problems with the cables). 

4. If sampling at depths other than the surface is required, a pre-cleaned Kemmerer 

water sampler or the Wheaton grab sampler will be used. 

5. Field preservation kits should be checked to verify that fresh preservatives have been 

obtained from chemistry stocks. Field preservation kits should contain the 

preservation acids to be used (in plastic measuring bottles), narrow range pH Litmus 

paper, and clean disposable plastic cups for measuring pH. 

6. The boat(s) should be fueled and clean, and all necessary safety gear packed. Boat 

batteries should be checked and placed on slow charge. All electrical (trim and tilt) 

and mechanical (steering) systems should be checked. 

7. The GPS unit should be checked and backup batteries secured. 

8. Station lists for each of the appropriate sampling units should be checked, and lists of 

sampling locations transferred to field notebooks. 

9. The field notebooks should be set up for the upcoming field collection activities (see 

Table 5.4). 

10. Field notebooks, list of potential sampling sites for each stratum in each reporting 

unit, waterproof pens, pencils, and markers should be placed in waterproof bags or 

containers. 

11. Enter the coordinates (longitudes and latitudes) of each of the upcoming sampling 

unit locations into GPS as way points. 

12. Charge multi-parameter sonde batteries.

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13. Check to see that there is sufficient water, prepared in one-gallon containers, of 

isotonic water in 5 ppt increments ranging from 0 to 35 ppt (if field staff is 

performing chlorophyll filtrations). 

5.3.2 Same Day Field Mobilization 

1. Check to see GPS is set for Eastern Standard Time. 

2. Calibrate multi-parameter sonde and complete all calibration sheets. If possible, a 

backup unit should be taken, as well as a complete calibration kit and minor repair 

kit. 

3. Field vehicle and boat should be filled with gas, and coolers filled with enough ice to 

transport samples during the course of collection. 

4. Dry Ice and/or freezer ice packs should be picked up for storage of the chlorophyll a 

samples (if field staff is performing chlorophyll filtrations). 

5.3.3 Surface Water Sampling 

1. Identification and documentation of sampling location - using the GPS unit use the 

GO TO function to sequentially proceed to each of the reporting unit sampling 

locations. (If the coordinates of the waypoints were not previously entered then input 

at this time.) 

2. The methodology used to locate the exact sampling site locations will differ slightly 

between the river segments and McKay Bay. 

• River Segments - Within each of the three reporting units (Hillsborough River, 

TBC/Palm River, and the Alafia River) the initial “go to” coordinates will be for 

randomly selected points along the centerlines of each river segment. Upon 

arriving at a point in the Hillsborough or Alafia River reporting unit, the field 

staff will visually divide the river cross-section into three equal segments from 

shoreline to shoreline. Based on predetermined random assignments for each 

river segment sampling location, the field staff will move the boat to either the 

left third (facing upstream), the middle segment, or the right third (facing 

upstream). After securely anchoring the boat, the field staff will then record the 

longitude and latitude coordinates in the field book and mark the location on the 

field map prior to beginning sampling. 

• TBC/Palm River - The field staff will follow a similar midline river segment 

procedure in locating the approximate upstream/downstream location. However, 

in this reporting unit the field staff will select either a shallow sampling stratum, 

less than 2 meters in depth (either Right or Left facing upstream), or a deep

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stratum. Depths are to be determined using a depth recorder or calibrated 

weighted line. 

• McKay Bay - Sampling locations will be based on randomly selected coordinates 

with predetermined assigned hexagons. If a hexagon sampling site cannot be 

collected (such as limitations due to depth restrictions), the field staff will select 

another location from the randomized list of potential sampling sites. 

3. If the sampling site cannot be sampled due to some unforeseen difficulty, move 20 

meters. If the site still cannot be sampled, move 20 more meters. If you have moved 

100 meters, implement the following step. 

4. If the area within 100 meters of the sampling site cannot be sampled due to some 

unforeseen difficulty, select the NEXT (NOT THE CLOSEST) station number for 

that stratum from the list of potential sampling sites provided for the current month. 

If the next station cannot be sampled, continue to select stations in the order they 

appear on the list, until a viable station is located. If no stations in a stratum can be 

sampled, proceed to the next stratum. Contact the project manager as soon as 

possible. 

5. Upon arrival at the sampling location: securely anchor the boat, place the multiparameter 

sonde over the side to equilibrate, and check the proper sample containers 

(as designated on the Chain-of-Custody Forms) for completeness. 

6. Record the actual site location (longitude and latitude in DDMM.MMM format) and 

arrival time in the field notebook (see Table 5.4). 

7. Provide a complete description of the station location and weather conditions, tide 

stage, and time (in Military/Eastern Standard) for each of the following sampling 

operations in the field notebook (see Table 5.4). Any unusual conditions are also to 

be noted (i.e. large numbers of dead fish floating, including species and approximate 

size range). 

Tidal stage will be listed as: 

• outgoing 

• incoming 

• high slack 

• low slack 

List wind speed as direction and approximate speed, mph will be converted to metric 

by computer. 

example: 10-15 mph NNW

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List cloud cover as percent: 

example: 30 % cloud cover, light rain 

Obtain air temperature using a separate thermometer 

example: 25 °C 

8. All water chemistry samples are to be collected from the upstream (and upwind, if 

possible) side of the boat, away from the motor. Chlorophyll a samples are to be 

collected in one liter, opaque polyethylene wide mouth bottles. 

9. Pre-cleaned bottles (as are typically received from Southern Analytical Laboratory) 

do not need to be rinsed prior to collecting the sample. Water samples from surface 

waters are to be collected by plunging the inverted container (neck down) quickly 

through the surface film to a depth of 0.2 meters (6 inches) and then tipping the 

container below the surface so the mouth faces up current until the bottle is filled and 

retrieving quickly to the surface. If sample bottles have not been pre-cleaned they 

should be rinsed twice with ample amounts of site water prior to collecting the sample 

(unless the bottle contains preservatives). Discard rinsate away from or downstream 

of the sampling location. If the sample bottle contains pre-measured preservative, 

submerge the unopened sample container to the appropriate level. Turn the container 

so that the opening is upright and the mouth faces up current. Open the container and 

allow the water to run into the container until almost full (leave an air space). Cap the 

container and return to the surface. Invert the container several times to ensure 

mixing of sample and preservatives. 

10. Should water samples at depths other than the surface be required, such samples are 

to be collected with a Kemmerer water sampler of sufficient size to collect enough 

water to both rinse and fill all of the sample bottles. If this is not possible, then a 

large pre-cleaned Teflon compositing container should first be filled, mixed and then 

used to fill the sample bottles. 

11. In collecting each group of chemistry parameters, the sample bottle number should be 

entered in the field notebook along with any additional notes regarding the sampling 

including preservatives and/or field filtration. Verify that the station number entered 

in the field book matches the sample number on the collection bottle. The collection 

time on the custody form should match the time of collection in the data book. The 

samples, other than chlorophyll a, should then immediately be place on ice in a 

designated cooler. Chlorophyll a samples must immediately be placed in a dark, 

cool, well-insulated ice chest. They must not be cooled below 4 °C (as a result, 

avoid direct contact with ice). 

12. Collect water column profile data (procedure outlined below) for: temperature; pH; 

specific conductance; salinity; and dissolved oxygen using the water column profiling

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methodology specified below. Note: water column profiles are to be done at half 

meter intervals except in: 1) the deep ship channel in Alafia River segment one and 2) 

the deep strata of the TBC/Palm River where profiles are to be taken at one meter 

intervals. 

13. Sample duplicates, both water column profiles and water chemistry, must be collected 

at the frequency described below (Section 5.4). 

14. Check multi-parameter sonde calibrations EVERY 4 HOURS for pH, specific 

conductance and DO. Record values in sonde calibration book. 

15. The Field Control Officer (FCO) should check all entries in the field notebook prior 

to the field staff leaving the sampling location. The FCO should also make sure that 

all recorded sample bottle numbers match those designated for each sampling 

location. Each member of the field staff should check all entries after completion of 

each sampling event, and sign and date each page of the field notebook. 

16. If “field filtration” of chlorophyll a samples is required, it should be done following 

the procedures outlined below after the completion of the day’s sampling run. The 

filtration should be done as soon as possible once the field staff has reached an 

appropriate low light environment. Both the time (Military/EST) of filtration and the 

volume filtered must be entered into the field notebook. Samples must be filtered and 

analyzed or frozen within 24 hours of collection. 

5.3.4 In-situ Multi-parameter Sonde Surface Water Sampling 

In-situ water column profile readings for temperature, dissolved oxygen, specific 

conductance, salinity, and pH are to be made from just beneath the surface (0.2 m) to just 

above the bottom (0.2 m) at 0.5 m intervals (unless otherwise specified). Unless weather 

conditions prohibit, calibrations will be checked every four hours during the sampling 

effort. If the values differ by more than 5% from the initial calibration standards then: 1) 

the sonde must be recalibrated; or 2) a calibrated backup unit should be used. All sondes 

used during field collections must be checked at the completion of each sampling run as 

part of the calibration procedures (see calibration sheet in Appendix 5-A). 

1. In the field notebook, record the date, time (Military/EST), sonde serial number, 

reporting unit, assigned sampling location and description. 

2. The sonde unit should be in the water stabilizing while the surface water quality 

chemistry is being collected. Arrange the sonde unit so that the probes are 

completely submerged at a depth of 0.2 meters. This depth must be based on 

previous markings made on the sonde unit. 

3. Wait until temperature has stabilized, then read and record: temperature, pH, specific 

conductance and salinity.

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4. Wait and verify the values for dissolved oxygen have stabilized before recording (this 

may take from 2 to 5 minutes depending on previous readings). Typically, dissolved 

oxygen levels will slowly decline when the sonde is lowered into waters with lower 

dissolved oxygen levels. Wait until the readings have stopped declining and have 

begun to fluctuate. 

5. Using pre-marked units on the sonde cable, frequently checking against depth 

recorded by the sonde, lower unit to required water column depths and repeat 

measurements. 

6. Conduct replicate water column profiles if required (see Section 5.4). 

7. The member of the field staff who records the entries in the field notebook should 

check all entries before proceeding to the next station. This person should also make 

sure that all sampling stations have been sampled and properly recorded. The Field 

Control Officer should review and sign each page of the field notebook before the 

pages are photocopied or entered in the database. 

5.4 Duplicates, Field Blanks and Split Sampling 

1. Conduct replicate water column profiles at a rate of at least 5% (1 out of every 20 or 

1 time per reporting unit per sampling day, whichever is greater). 

2. Duplicate surface water quality grab samples will be collected at a rate of 5% or once 

per sampling event, whichever is greater, in order to estimate field sampling 

precision. 

3. Field Blanks will be prepared using analyte free water, to assess both potential infield 

contamination and reported minimum laboratory detection limits. 

4. Split Samples may be collected as part of the overall quality assurance plan and used 

to assess the accuracy of reported laboratory results. The collection of split samples 

will be from consecutive sample volumes from the same sampling device to attain 

two samples as identical as possible. All other variables of collection and analysis 

will be held as constant as possible including the same types of containers cleaned in 

the same manner, the same preservatives in equal amounts, and the same method of 

analysis and instrumentation. Additionally, staff members participate in quarterly 

Southwest Florida Regional Ambient Monitoring Program (RAMP) sampling where 

split samples are obtained and analyzed by multiple laboratories in the region.

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5.5 Field Preservation 

1. If preservation is required preservative should be added from the marked dropper 

bottle at the approximate rate of 2 ml per liter 4N H2SO4 (reagent grade or better) for 

nutrients, 2 ml per liter 4N HNO3 (reagent grade or better) for metals and 0.2 ml of 

6N HCL (technical grade) for carbons. 

2. After adding 1.0 ml, the sample bottle should be thoroughly shaken, a small 

subsample taken in a small clean sampling cup and the pH checked using narrow 

range pH paper. DO NOT insert the pH paper into the sample bottle. This step 

may be repeated if necessary until the proper pH range (normally < 2) is reached. 

3. The same amount of preservative should be added to the equipment blank and 

recorded in the field notebook. If extra acid is used, the amount is to be noted in the 

field notebook. 

4. Additional chemical used to augment field preservation will be from the same source 

as the chemical used to preserve the sample. 

5. Samples will not usually be collected in pre-preserved containers. In general, samples 

requiring preservation will have parameter specific preservatives added in the field. 

Under certain weather conditions, controlled preservation cannot be accomplished 

without contamination of the sample or risk to the field sampler working with acids, 

i.e., during rain, in the dark, in rough waters. When these conditions exist, the 

samples will be held on ice in coolers and preservation will be done as soon as the 

collection is completed and the boat has returned to the dock. The time of collection 

and the time of preservation will be noted in the field book. 

5.6 Chlorophyll a and “Field Filtration” 

Under normal sampling procedures, chlorophyll a samples will be collected and 

couriered (or shipped) to arrive at the laboratory on the same day that they are collected. 

However, if there are any problems getting the samples to the laboratory for filtration on 

the same day as collection, additional steps must be taken with the chlorophyll a samples. 

Due to their very short holding time, samples must be immediately frozen, using the 

following procedures, if they cannot be delivered to the laboratory and filtered on the 

same day as they are collected. 

1. A one-liter subsurface sample will be collected in accordance with the water quality 

chemistry procedures specified above. Sampling must be done using opaque or 

“dark” polyethylene bottles to eliminate exposure to light after sample collection. 

2. The chlorophyll a samples will immediately be placed in a cool, dark ice chest. 

Under no conditions should the chlorophyll a sample containers come into direct

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contact with ice water (since this could cause the rupture of phytoplankton cells due 

to temperature stress). 

3. Samples will be delivered to, and filtered by, the laboratory on the same day that they 

are collected. However, if this is not possible, then the chlorophyll a samples must be 

field filtered, in a low light environment, and frozen prior to being shipped to the 

laboratory. This must be done as soon as possible after completion of the sampling 

event. 

4. Prior to filtering, the one-liter sample will be gently shaken by repeated inverting of 

the container in order to resuspend any settled phytoplankton. 

5. A sub-volume will then be taken using a 500 ml polypropylene graduated cylinder. 

The volume that can be filtered will depend on the amount of plankton and other 

suspended materials in the water column. The amount filtered should be the greatest 

amount that can practically be filtered, up to a sub-volume of 500 ml. 

6. This volume will then be gently filtered through a 47 mm diameter, 0.45 micron 

cellulose nitrate membrane filter using a standard one-liter polypropylene filtering 

flask, a 500 ml Gelman magnetic filter holder, and a Nalgene hand pump for suction. 

The vacuum applied using the hand pump should never exceed 10- 20 inches Hg (50- 

68 kPa). 

7. The sides of the filtering flask will then be rinsed using an approximately isotonic 

solution made from analyte free water and commercial grade salt. (One-gallon 

containers of isotonic water will have been previously prepared in 5 ppt increments 

over a range of 0 to 35 ppt.) 

8. Two drops of saturated magnesium carbonate solution will be added to the final rinse, 

immediately prior to the completion of filtering. 

9. The filter, with the trapped phytoplankton, will then be quickly removed, folded, and 

placed in 15 cm coarse paper filter that is then also folded in half. The larger filter 

paper must be pre-labeled in pencil, with the sample ID, date, time, initials of 

sampler, and the exact volume filtered. A paper clip can then be used to hold the 

filters together. 

10. All of the filters are then to be placed in a re-sealable plastic bag and placed between 

two frozen freezer packs, and placed in an appropriate cooler. 

If surface water quality parameters such as dissolved concentrations of trace metals or 

ortho-phosphates are required in the future, these samples cannot be preserved until 

filtered. Such samples should be field filtered using a clean syringe and 0.45 micron glass 

filter contained in a Teflon filter holder (with stainless steel screen) that has been cleaned 

and prepared at the laboratory. At least one cleaned filter and syringe combination with

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extra filter papers should be provided for each station, when possible, to avoid field 

cleaning. Samples should then be preserved after filtering. 

5.7 Sample Container and Equipment Cleaning Procedures 

The specific procedures outlined herein will be taken to assure appropriately prepared 

sample containers, and provide for the necessary cleaning and decontamination of 

sampling equipment for each chemistry parameter. 

5.7.1 Laboratory 

All sample containers used will be obtained from Southern Analytical Laboratory after 

they have been pre-cleaned for each of the specific water chemistry parameters to be 

collected. All such containers will be cleaned and certified as such following the 

procedures specified in the laboratory’s Quality Manual. 

5.7.2 Decontamination of Field Equipment 

After each field trip, multi-parameter sondes will be checked, cleaned and stored as 

detailed in the calibration procedures. 

It is anticipated that all subsurface water quality samples will be collected without the use 

of any equipment, thus eliminating the need for cleaning and decontamination of field 

equipment between sampling locations. However, decontamination will be required in 

the event that future or special monitoring of water chemistry parameters requires the use 

of sampling equipment, such as Kemmerer samplers, buckets, dredges, etc. In 

accordance with the EPA SOP and QAM (1996), Section No. B. 8.2, such sampling 

equipment used for routine sample collection may be cleaned with sample water or deionized 

water between sampling locations. If necessary, a brush may be used to remove 

deposits of material or sediment. If de-ionized water is used, the rinse should occur at the 

next sampling location before the sample is collected. 

Such field cleaning procedures will be used to clean the Field Filtration apparatus used to 

filter samples for chlorophyll a analysis between sampling locations. 

5.8 Sample Transportation and Shipping Procedures 

In general, field personnel will be responsible for sampling and either transferring the 

samples to a courier service or transporting the water quality samples back to the PBS&J 

Tampa office. In almost all instances, samples will be transported either under the direct 

control of the field crews or the courier service to Southern Analytical Laboratory. 

However, in those rare instances where the field crews use other methods to meet 

required holding times, the procedures outlined below will be followed.

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1. Prior to leaving the sampling site, the field crews will check to make sure that the 

sample bottles are sorted by sampling location and parameter group and that 

identifications on the bottles match those recorded in the field notebook. 

2. Bottle types, preservation, etc. are to be checked in accordance with the procedures 

outlined in Table 5.5. 

3. The time of sample collection, etc. should be documented as required in both the 

Field Data Sheets (Table 5.4) and Chain-of-Custody Forms (Appendix 5-A). 

4. All bottle tops will be checked to make sure they are tight. 

5. Samples will be secured in appropriate coolers on sufficient wet-ice to insure proper 

cooling. However, in no instance shall bottles be placed in such a manner as to allow 

floating once the ice begins to melt. 

6. Coolers will be transported back to the PBS&J staging area in secured vehicles under 

the direct control of the field staff. Vehicles will be locked whenever unattended, 

even for brief periods of time, to assure possession by field crews. 

7. Field notebooks and Chain-of-Custody records for each group of samples must 

accompany the appropriate coolers. 

8. Field crews must check the condition of the coolers periodically (at least once every 

two hours). Any water should be drained from the bottom of the coolers and 

sufficient new wet-ice added to insure proper cooling. 

9. Field crews are responsible for checking in all samples and transferring Chain-of- 

Custody records to Southern Analytical Laboratory, either directly at the lab or via a 

courier for the lab. Field crews are responsible for documenting specific 

conductance, pH, temperature, and collection time for each sample on the Chain-of- 

Custody form. 

5.9 Sample Custody 

5.9.1 Objectives 

• To provide a detailed and legally defensible Chain-of-Custody through the entire 

process of preparing sample containers, shipment to the field staff, sample collection, 

shipment back to the laboratory, and chemical analysis. 

• To provide the field staff complete sets of certified pre-cleaned, pre-labeled sample 

containers for each required sampling site.

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• To be able to accurately track the time, date and location of all water quality samples 

collected during the HBMP. 

• To assure that all analytical results are matched correctly to the appropriate sample 

containers. 

5.9.2 Sample Custody - Overview 

PBS&J field and Southern Analytical Laboratory staff will maintain Chain-of-Custody 

procedures in order to document that all samples are in possession, view, or stored 

securely at all times. 

Prior to each sampling event, unique identification numbers will be assigned to each 

sampling location within each of the estuarine/riverine systems. All station locations, 

parameters, and sampling schedules will be pre-determined as part of the randomized 

temporal and spatial monitoring design, and will be entered into the Laboratory’s 

Information Management System. The Laboratory’s software package is used for the 

routine pre-logging of samples, calculation and storing of analytical results, generating 

reports, and reporting quality control data. After the pre-logging procedure is proofed, 

labels are affixed to the necessary types of sample containers. 

Sample labels may be preprinted for the following fields of information: 

1. Date collected 

2. Client 

3. Project name 

4. Site description 

5. Analysis requested 

6. Type of sample that should be collected in the container (Nutrients, Metals, etc.) 

7. Type of preservative that is in the container e.g. HNO3, H2SO4, HCL, No Chemical 

Preservative, etc. 

Southern Analytical Laboratory in Oldsmar will originate appropriate Chain-of-Custody 

Forms (Appendix 5-A) for each of the sampling events for each of the estuarine systems 

prior to sample collection. Based on this Chain-of-Custody, pre-cleaned containers will 

be prepared and made ready for sample collection. A Sample Kit Request form directs 

the preparation of all sample containers, labeling of such, documentation of sample bottle 

and preservative lot identifications, shipment information, and any ancillary comments. 

Sample containers for all of the water quality parameters to be collected at each sampling 

location will be packaged separately. These sample containers, including those required 

for both duplicate and field blanks will be placed in individually marked coolers specific 

for each of the estuarine systems. These coolers will then be picked up by or shipped to 

the field collection staff at the PBS&J Tampa office.

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Prior to sample collection, all of the sample containers for each of the estuarine systems 

will be checked for both completeness and label accuracy by the personnel responsible 

for the field collections. Any problems will immediately be reported to both the 

Laboratory and project QA/QC Officer. The field leader will be responsible for 

confirming that complete, prepared containers have been received from the Laboratory by 

acknowledging receipt on the appropriate Sample Tracking/Chain-of Custody Forms. 

The field leader will also be responsible for making sure that additional information 

required on these forms is completed and correct. 

This additional information should include: 

1. What containers are field preserved and with what preservative. 

2. What parameters and/or containers are field filtered. 

3. How the samples are to be transported. 

All samples arriving at the Southern Analytical Laboratory will be immediately checked 

for potential problems, such as: 

1. Leaking, broken or empty sample containers. 

2. Missing containers. 

3. Damaged or otherwise unreadable labels. 

4. Improperly preserved containers. 

Any problems will be noted on a Standard Quality Control Form by the sample 

custodian. This form is signed by the originator, the Laboratory Quality Assurance 

Officer, and the laboratory project manager. The Form is then transmitted to the PBS&J 

Project Manager and/or HBMP QA/QC Officer. It will be the responsibility of the 

Project Manager or QA/QC Officer to determine the feasibility and/or appropriateness of 

recollecting damaged or lost samples, or proceeding with laboratory testing. 

Once the Laboratory Sample Custodian has established that all samples documented on 

the Chain-of-Custody/Sample Tracking Forms have reached the Laboratory intact, they 

will countersign the Chain-of-Custody taking responsibility from the Field Sample 

Control Officer. 

Collection data from both the Chain-of-Custody and copies of the Sample Field 

Notebook will also be entered into the Laboratory Information Management System 

(LIMS) and a Laboratory Confirmation of Work Received will be generated with the 

unique log-in identifier. 

Sample containers will then be distributed to the appropriate sample storage area, and 

appropriate Internal Sample Tracking Forms will be generated by the LIMS. The 

samples are stored, and documented as such, in a designated storage location, under 

appropriate storage conditions, until all the measurements are complete. All sample 

preparation and analyses are recorded in individual staff laboratory notebooks, and also

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on extraction/digestion/analyses preparation and run logs. The internal sample tracking 

procedures also contain information on final sample disposal. This system ensures that 

all samples in existence in the laboratory are either in identifiable storage areas or in the 

possession of an identifiable chemist. 

5.10 Field Sample Control Officer 

The designated Field Sample Control Officer will be designated as the senior staff in 

charge of a specific sampling run. In the field, the designated Field Sample Control 

Officer ensures that: 

1. Upon arrival at the sampling location: the boat is securely anchored, the multiparameter 

sonde is placed over the side to equilibrate, and the proper sample 

containers (as designated on the Chain-of-Custody Forms) are taken out and checked 

for completeness. Sampling crews should always carry at least two complete sets of 

labeled backup sample containers, which can be used if any of the designated sample 

containers are lost or contaminated during the sample run. 

2. Proper sample collection, handling and preservation methods are observed. 

3. All appropriate containers are filled at each sampling site. 

4. Collection times and each sample number are written into the field notebook. 

5. All necessary written documentation, including weather notes, tide stage, etc. are 

recorded at each station location. 

6. Upon completion of collection of the water chemistry samples, all container lids are 

securely closed and the sample containers are properly stored in the appropriate 

coolers. 

7. In situ water quality measurements are made, recorded and checked for 

reasonableness. 

8. All equipment is properly stored and the field notebook is placed in a secure location 

before leaving the sampling site. 

Strict adherence to these procedures will assure that sample container mix-up in the field 

is nearly impossible. 

5.11 Field Custody and Documentation 

All field records are to be kept in bound, waterproof field notebooks. Notes are made in 

permanent waterproof ink or pencil if necessary. Wet weather conditions often preclude 

use of ink in the field and pencil must be used. Corrections are accomplished by crossing 

out the incorrect data with a single line and writing in the correct data. Records in the 

field notebook are never to be erased or altered other than by making a single mark 

through them. This includes any data collected during sampling runs that are 

discontinued (such as for mechanical failures or the development of dangerous weather 

conditions). Corrections are initialed by the personnel making the correction and dated, 

if not made on the same date as the collection. Field records include:

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1. Collection date and time. 

2. Total water depth. 

3. Sampling location identification (station number). 

4. Sequential sample numbers (the samples may not be sampled in sequential order but 

as a sample is collected the collection data is recorded for that sample number). 

5. Field measurement data. 

6. Depth(s) of measurement(s). 

7. The number and types of QC samples collected. 

8. Collection time reflecting the sequence of sample collection. 

9. Designation of multi-parameter sonde used. 

10. Any filtering and/or sampling equipment used. 

11. If field filtration is required and a record of which sample containers were filled for 

each station. 

12. Weather and tide data. 

13. Equipment problems. 

14. Notes about the sampling location, including station maps as necessary 

15. Type of preservative used and sample pH checked to see if additional preservative is 

required. 

Field notebooks are to be signed by all personnel involved in a sampling event and all 

visitors on site must be identified. All sample containers are pre-labeled with project 

number, station number, sample number and lab identification number; this information 

is to be transferred into the field notebook at the time of collection. 

When boats with fuel powered engines are used, the samples are always collected 

upstream/tide of the engines and from the bow of the boat away from the engine. 

Upon return from the field all notebook pages will be checked for completeness and 

signed by the Field Sample Control Officer. This person is also responsible for checking 

to make sure that all samples have been collected, are tightly sealed, and properly packed 

for transfer to Southern Analytical Laboratory. The Field Sample Control Officer will 

then sign the Chain-of-Custody/Sample Tracking Forms and see that the coolers are 

properly sealed and immediately transferred to the Laboratory so that all water quality 

parameters can be analyzed within their designated holding times. If the samples are 

picked-up in the field during a long-term study in which the field notebook will stay in 

the field, the person transporting the samples to the laboratory will sign both the Chainof-Custody 

and the field notebook. 

After appropriate information from the Chain-of-Custody Forms has been entered into 

the computer database, two separate sets of files will be maintained. An original Chainof-Custody 

(COC) Record will be kept in the Laboratory Data Management files. If the 

COC is a multipart form, one of the copies is returned to the designated “report to” 

project person with the Certificate of Analysis, and a copy of the COC is also transmitted 

with the Confirmation of Work Received. The Tampa office of PBS&J, will be 

maintaining this information separately as part of the permanent project files.

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5.12 Laboratory Sample Control 

Laboratory sample integrity is the charge of a designated Sample Custodian. These 

duties include: 

1. Initiating Chain-of-Custody Forms required for shipment with each set of sample 

containers. 

2. Assuring that pre-logged and pre-labeled sample containers are prepared and shipped 

with appropriate Chain-of-Custody and Tracking Forms to the field sampling 

personnel. (In specific instances include adding any required preservatives to 

containers.) 

3. Verifying and signing for incoming field samples, signing Chain-of-Custody Form 

from field staff. 

4. Beginning Internal Chain-of-Custody forms for each batch of samples. 

5. Logging samples into appropriate storage areas where they are then available for 

chemistry analysts and technicians. 

6. Maintaining complete files of all the Chain-of-Custody records. 

7. Overseeing the collection and maintenance of the sample custody sheets after 

individual Chemists have disposed of their samples. 

5.13 Laboratory Custody 

Samples arriving at Southern Analytical Laboratory will first be checked for proper 

identification, completeness, preservative, documentation on Chain-of-Custody Forms, 

and sample integrity (leaks, temperature, etc.). If any problems exist the Sample 

Custodian initiates a sample custody quality control protocol, and this information is 

immediately brought to the Laboratory QA Officer for review, as well as to the attention 

of the Laboratory Project Manger. The Project Manager and/or the Project QA/QC 

Officer in the Tampa office is then contacted, and documented determinations as to the 

need for re-sampling will then be made. Once the samples have passed this initial check, 

the sampling information will be recorded into the Receiving Records of the LIMS. 

Information recorded includes: collection date and time, field sampler identification, 

project number, station descriptors, type of sample, and parameters to be analyzed, field 

measurements, special notes. Using this information the computer then generates a 

Laboratory Confirmation of Work Received for that sampling event’s log-in. Additional 

information such as date (time also if

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The log-in unique identifier with field samples received is then immediately associated 

with the daily work group records available to each Technical Coordinator and 

Analyst/Technician in the laboratory. Each log-in identifies when samples were taken in 

the field so that recommended holding times are not exceeded. Assigned completion 

dates are listed on the log-in, along with any project specific requirements. Batch Quality 

Control samples, such as method blanks, laboratory control samples, matrix spike and 

matrix spike duplicate samples are also assigned. Technical Coordinators and laboratory 

Project Managers review daily sample backlogs for status on project log-ins. 

When a specific parameter for a sample is completed the value is entered into the 

computer database either directly or for further calculation if necessary. Once entered, it 

is proofed by the respective Technical Coordinator against the raw and summary data to 

validate the original entry. If the entered data are not correct, the necessary changes are 

made and the results returned for data editing. If a problem is found to exist, the QA/QC 

Officer and the laboratory Project Manager are notified. Each summary data package 

contains an example of each calculation performed for data reporting. When all the 

analyses have been completed for a specific set of samples, a report is generated. A 

cover letter attesting to the authenticity of the results is attached to this report, which will 

be sent to the Tampa office of PBS&J. The Laboratory will also maintain secure copies 

of the report. The Southern Analytical Laboratory’s Information Management System is 

designed around, and operates on Good Automated Laboratory Procedures. 

Complete and detailed descriptions of all Southern Analytical Laboratory operating 

procedures and protocols are contained in the most recent Department of Environmental 

Protection (DEP) approved Laboratory’s Comprehensive Quality Assurance Plan, and 

documentation associated with the Laboratory’s certification (E84129) by the Florida 

Department of Environmental Protection (FDEP) and Health (FDOH) for the analyses of 

environmental water quality samples. 

5.14 Preventative Maintenance 

5.14.1 Field Instruments/Equipment 

Most of the field sampling equipment and instruments are to be used frequently enough 

that normal maintenance will be done in conjunction with pre-mobilization and 

calibration procedures. In addition, all equipment is to be cleaned and checked after each 

use and any required maintenance done at that time. 

• Routine maintenance of multi-parameter sondes will be documented on the 

calibration sheets and permanently kept on file by the QA/QC Officer. 

• All non-routine repairs are to be documented in staff personal field books. Any 

invoices/receipts will be kept on file in the project office.

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In the event of a major equipment failure, the options to be followed are prioritized in the 

order enumerated below. Decisions are to be based on the highest level of data collection 

attainable as assessed by the Field Sample Control Officer present at the time and place 

of the failure. (Any such activities must be noted in the field notebook.) 

1. Use backup equipment. PBS&J and other members of the HBMP study team will 

each maintain a full array of field sampling equipment (boats, GPS units, multiparameter 

sondes, etc.). All field crews will carry cell phones and a list of contacts 

able to provide backup sampling equipment in order to reduce down time from 

equipment failure. 

2. Postpone sampling, if appropriate backup equipment is not readily available, until 

repairs are made on the equipment or other sampling arrangements can be made. 

3. Collect grab samples and analyze using laboratory instruments upon return to the lab. 

(Example: conductivity probe fails and cannot be corrected in the field. Grab samples 

are to be collected near the surface and near the bottom.) 

4. Invalidate data. Repeat or report as N/A any invalidated data from the field. 

5.15 Multi-parameter Sonde Operation and Calibration 

5.15.1 Introduction 

The multi-parameter sondes must be checked and calibrated immediately prior to their 

use to assure both the accuracy and precision of in situ field readings (see Table 5.6). At a 

minimum, the units must be thoroughly checked and calibrated within 24 hours of use. If 

during calibration, the readings of known solutions are found to be outside the 

predetermined range of accuracy, the calibration must be repeated and the instrument 

readings determined to be stable and reliable prior to use. All calibration sheets must be 

permanently maintained in a three ring binder. 

The in situ parameters that are measured by these instruments include: 

• temperature 

• salinity 

• specific conductance 

• dissolved oxygen 

• pH 

• depth (values should be checked prior to each reading against predetermined 

measurements marked on the data sonde cable)

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The accurate recording of these data is important because they will be used to assess 

long-term temporal and spatial variations in water quality within the sampled 

estuarine/riverine systems. 

5.15.2 Objectives 

• Calibrate units properly to ensure accurate readings. 

• Accurately record hydrologic data at each sampling site. 

• Check in situ data after entry into project database. 

5.15.3 Procedures 

A) General multi-parameter sonde Operation Procedures 

1. Before leaving the office, check the voltage on the display of the unit. A fully 

charged unit should have approximately 14 volts. However, as a battery gets older, a 

fully charged battery may not reach 14 volts. All units should be evaluated on an 

individual basis. Calibration sheets and written records should specify the voltage 

each unit will hold and, most importantly, the approximate voltage when each unit 

will stop working. Always take spare batteries. 

Note: Whenever possible bring a complete (and calibrated) spare unit on all sampling 

events. The units can and do become inoperable. At a minimum, salinity (specific 

conductance) and temperature should be taken at all sampling sites. If these two 

parameters cannot be measured all sampling should be discontinued until a 

replacement unit is obtained. 

2. Keep the probes immersed in water at all times. Exposure to air will dry out the 

membranes, which, in turn, will give false readings. 

3. Once on station, turn the unit on and allow it to equilibrate before taking the readings. 

4. Take in situ water quality readings at the surface (0.2 meters below) and bottom (0.2 

meters above), and at 0.5 meter intervals in between (unless otherwise noted). 

5. Rinse and wipe down the units upon returning to the office. Rinse the probes with 

clean, de-ionized water. Fill the probe storage cup with de-ionized water, and clean 

all cables and the surface units. 

6. Allow units to thoroughly dry before being packed in their storage cases.

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B) YSI Setup, Calibration and Maintenance 

1. Do Membrane Installation (YSI 600 Series) - position the sonde in a vertical 

position such that the sensors are upright. Remove the probe guard from the sonde. 

Remove the old DO membrane and clean the probe tip with water and lens cleaning 

tissue. Make sure to remove any debris or deposits from the O-ring groove. Using a 

dropper bottle, fill the DO probe with new electrolyte. 

Hold the membrane so that all four corners are supported, but do not stretch the 

membrane laterally. Position the membrane over the probe, keeping it parallel to the 

probe face as shown above. With one continuous downward motion, stretch the 

membrane over the probe face as shown in the pictures. Do not hesitate to stretch the 

membrane. 

Install a new O-ring by placing one side of the O-ring in the groove and rolling into place 

across the membrane and into the groove on the opposite side of the probe face. Avoid 

touching the probe face with your fingers. Once the O-ring is in position, squeeze it every 

90 degrees to equalize the tension. Do not use grease or lubricant of any kind on the 

O-ring. 

Observe the following cautions to assure that the membrane is installed properly:

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• Secure the sonde tightly so that it will not move during membrane installation. 

• Wash hands before installation and do not allow finger oils or O-ring lubricant to 

touch the probe face or the membrane. 

• Use caution when replacing the probe guard that you do not touch the membrane. If 

you suspect that the membrane has been damage, replace it immediately. 

2. Sonde Calibration - the calibration cup that comes with the sonde serves as a 

calibration chamber for all calibrations and minimizes the volume of calibration reagents 

required. If a sonde with a wiper is being used it should be visually inspected to assure 

proper movement of the mechanism before beginning the calibration procedures. 

Instead of the calibration cup, standard laboratory glassware may be used to perform 

calibrations. However, if the calibration cup that is designed for the sonde is not used, the 

following cautions should be used: 

• Perform all calibrations with the Probe Guard installed. This will protect the probes 

from possible physical damage. 

• Secure the sonde body to prevent the sonde from falling over. 

• Ensure that all sensors are immersed in calibration solutions. Many of the calibrations 

require immersion of other probes (e.g., the temperature probe). The top vent hole of 

the conductivity sensor must also be immersed during calibrations. 

The following tips should be employed as necessary during calibration using the YSI 

calibration cup: 

• Remove the probe guard, if it is installed. 

• Sonde calibration can be accomplished either with the sonde upright or upside down. 

However, it should be supported to prevent damage during calibration. 

• Make certain that port plugs are installed in all ports where probes are not installed. It 

is extremely important to keep these electrical connectors dry. 

• Ensure that a gasket is installed in the gasket groove of the calibration cup bottom 

cap, and that the bottom cap is securely tightened. Note: Do not over-tighten as this 

could cause damage to the threaded portions of the bottom cap and tube. 

• When calibrating for dissolved oxygen (DO) utilizing the saturated air method, make 

sure to loosen the seal of the calibration cup and allow the pressure to equilibrate 

before calibration, since DO calibration requires water-saturated air.

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• Using the Calibration Cup for dissolved oxygen calibration, make certain that the 

vessel is vented to the atmosphere by loosening the bottom cap or cup assembly and 

that approximately 1/8” of water is present in the cup. 

• Other than during DO calibration, be sure that the sensors are completely submersed 

when calibration values are entered. 

• Be sure to use sufficient volumes of solution when performing calibrations. 

• When available, utilize previously used, stored calibration solutions to rinse the 

sonde, prior to each calibration procedure. 

• Ambient temperature freshwater can also be used to rinse the sonde between 

calibration solutions. 

• Be sure to remove the stainless steel weight from the bottom of the sonde prior to 

calibration in order to reduce both amount of calibration solutions and the amount of 

liquid that is carried between calibrations. 

• Be sure to shake excess rinse water off of the sonde, especially when the probe guard 

is installed. Additionally, carry-over can be further reduced by using clean, absorbent 

paper towels or cotton cloths to dry the sonde between rinses and calibration 

solutions. 

The following outlines normal calibration procedures for the sensors commonly used 

during the HBMP monitoring program. In order to ensure accurate calibration, rinse the 

calibration cup with water first and then rinse it again with a small amount of the 

appropriate calibration solution. Discard the rinse solution and add sufficient fresh 

solution. Carefully immerse the probes into the solution and rotate the calibration cup to 

engage enough threads to prevent leaking (Do not over tighten). 

The appearance of the menu will vary depending upon the sensors enabled on the sonde. 

To select the parameter from the Calibrate menu, simply input the number that is next to 

the parameter. Once you have chosen a parameter, some of the parameters will have a 

number that appears in parentheses. These are the default values and will be used during

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calibration if you press Enter without inputting another value. Be sure not to accept 

default values unless you have assured that they are correct. If no default value appears, 

type a numerical value and press Enter. A real-time display will appear on the screen. 

Carefully observe the stabilization of the readings of the parameter that is being 

calibrated. When the readings have been stable for approximately 30 seconds, press 

Enter to accept the calibration. Press Enter again to return to the Calibrate menu, and 

proceed to the next calibration. 

3. Conductivity - this procedure calibrates conductivity, specific conductance, salinity, 

and total dissolved solids. Before proceeding insure that the sensor is as dry as possible. 

Ideally, rinse the conductivity sensor with a small amount of standard that can be 

discarded. Be certain that you avoid cross-contamination of standard solutions with other 

solutions. Make certain that there are no salt deposits around the oxygen and pH probes, 

particularly if you are employing standards of low conductivity. 

Place sufficient conductivity standard into a clean, dry or pre-rinsed calibration cup. Be 

certain to immerse the entire sonde in solution standards for calibration of all 

parameters. Most calibrations require readings not only from the sensor being calibrated 

but also from the temperature sensor. Carefully immerse the probe end of the sonde into 

the solution. Gently rotate and/or move the sonde up and down to remove any bubbles 

from the conductivity cell. The probe must be completely immersed past its vent hole. 

Allow at least one minute for temperature equilibration before proceeding. 

From the Calibrate menu (as shown above), press 1-Conductivity and the following 

display will be shown calling for your numerical input. 

The number in parentheses is the default value of this parameter and will be used in the 

calibration only if Enter is pressed without typing in another value. Similar prompts will 

be displayed during the calibration of all parameters, but for some sensors, such as pH, no 

default values are provided. In these cases, the user must input a numerical value and 

then press Enter. 

After the calibration value is inputted and Enter is pressed, a real-time display similar to 

the following will appear on the screen.

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All of the parameters that have been enabled will appear - not just the one being 

calibrated at the moment. Carefully observe the stabilization of the readings of the 

parameter that is being calibrated and, when the readings are stable for approximately 30 

seconds, press Enter to implement the calibration and the following message will appear. 

If an ERROR message appears, begin the calibration procedure again. Be certain that the 

value entered for the calibration standard is correct. 

Conductivity should be calibrated with a standard at the high end of the range of field 

values expected. An additional check of conductivity should be completed using a 

standard at the low end of the range of expected field values. 

4. Dissolved Oxygen 

600 Series with Rapid Pulse Polarographic Dissolved Oxygen (Membrane) 

If the DO sensor has just been resurfaced, it is recommend the probe be run continuously 

for 15-30 minutes or until good stability has been achieved. After simply changing a DO 

membrane (not resurfacing), it is usually only necessary to run the probe continuously for 

3-4 minutes to achieve stability. 

To calibrate with the saturated air method, place approximately 3 mm (1/8 inch) of water 

in the bottom of the calibration cup. Place the probe end of the sonde into the cup. Make 

certain that the DO and temperature probes are not immersed in the water. Engage only 1 

or 2 threads of the calibration cup to insure the DO probe is vented to the atmosphere. 

Wait approximately 10 minutes for the air in the calibration cup to become water 

saturated and for the temperature to equilibrate. 

All of the sonde sensors (except temperature) require periodic calibration to assure 

required accuracy. However, the calibration protocols for dissolved oxygen are 

significantly different depending on whether the sonde is being set up for spot sampling 

or longer term unattended monitoring studies. This difference is selectable and is required 

primarily because the optimal performance of the Rapid Pulse dissolved oxygen sensor 

cannot be attained unless the control of this sensor varies from short term to long term 

applications. When spot sampling is required, it is best to pulse the sensor continuously 

during the Run mode to attain the most accurate results and optimize the response time. 

However, this continuous pulsing is not ideal for longer term logging studies in which the 

sonde data is captured to sonde memory or to a data collection platform at much less 

frequent intervals (e.g. 15 minutes). Continuous pulsing not only shortens the time 

between required probe maintenance, but consumes more power. With proper selection 

of the “Auto sleep” option the sonde software can be configured to either run 

continuously or “go to sleep” between samples to prevent DO probe wear and conserve 

power. The effect of this choice on the user interface relative to dissolved oxygen

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calibration is significant. If the instrument is being used in sampling applications where 

the dissolved oxygen is “on” continuously during the sampling (such as during 

conducting in situ water column profiles) deactivate “Autosleep RS232”. 

If “Auto sleep” is deactivated, the sonde runs continuously no matter what sample 

interval has been selected. Under these conditions, the user retains manual control of the 

dissolved oxygen calibration routine, viewing the stabilization of the readings in real time 

and confirming the calibration with keyboard entries. 

If “Auto sleep” is activated, the sonde will ‘warm up’ the sensors for the period of time 

selected for the DO sensor. Under these conditions, the user loses manual control of the 

DO calibration routine. DO will automatically calibrate after the selected time for warm 

up of the DO sensor has expired. In this mode of calibration, you do not observe 

stabilization of the readings in real time, but instead will observe a countdown of the 

warm up period followed by a message indicating that the DO calibration is complete. 

Only the calibration of dissolved oxygen is affected by whether “Auto sleep” is on or off; 

the user retains manual control of the calibration of all other parameters regardless of the 

“Auto sleep” setting. Once a particular warm up time (in seconds) has been utilized in 

DO calibration, the length of that time should not be changed during a study. A new 

calibration should be performed whenever the value of the warm up time is altered. 

Remember, the Calibration Cup is designed to be air-tight and must be loosened if it is 

used as a calibration chamber. From the Calibrate menu, select Dissolved Oxy, then 

select DO % to access the DO percent calibration procedure. Calibration of dissolved 

oxygen in the DO % procedure also results in calibration of the DO mg/L mode. 

For the percent saturation mode, be certain that the sensor has been thermally equilibrated 

in water-saturated air and that the sensor shows stable readings prior to beginning the 

calibration routine. This is particularly important after changing the membrane. It is 

important that the sensor be “off” for at least 5 minutes before initiating a calibration 

procedure with “Autosleep” active. Ideally the sensor should be “off” for a period of time 

equal to the sample interval that will be used over a long-term deployment. 

Enter the current barometric pressure in mm of Hg. (Inches of Hg x 25.4 = mm Hg). 

Laboratory barometer readings are usually “true” (uncorrected) values of air pressure and 

can be used “as is” for oxygen calibration. Weather Service readings are usually not 

“true” since they are corrected to sea level, and therefore cannot be used until they are 

“uncorrected” ( for example True BP = [Corrected BP] – [2.5 * (Local Altitude/100)]). 

However, since all Tampa HBMP data is collected very near sea level either actual field 

or local Weather Service barometric values may be used. 

The DOsat % (percent air saturation) value will reflect the entered barometer value. For 

example, if a barometric pressure of 720 mm is entered, the DOsat % value will change 

to 94.7 % (720/760 x 100). A “DOsat %Local” option is also available to set their percent 

air saturation value to 100%, no matter what barometer value is entered. Remember that

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no matter which convention (DOsat % or DOsat %Local) is selected, the mg/L value 

ultimately should remain the same. 

Press Enter and the current values of all enabled sensors will appear on the screen and 

change with time as they stabilize. Observe the readings under DO%. When they show no 

significant change for approximately 30 seconds, press Enter. The screen will indicate 

that the calibration has been accepted and prompt you to press Enter again to return to 

the Calibrate menu. 

If your instrument will be used in monitoring applications where data is being captured at 

a longer interval (e.g. 15 – 60 minutes) to internal sonde memory or data collection 

platform activate “Autosleep RS232”. With Autosleep active, the calibration will occur 

automatically with a display similar to that shown below. 

YSI 6920 with Rox Optical Dissolved Oxygen 

Place the sensor either (a) into a calibration cup containing about 1/8 inch of water which 

is vented by loosening the threads or (b) into a container of water which is being 

continuously sparged with an aquarium pump and air stone. Wait approximately 10 

minutes before proceeding to allow the temperature and oxygen pressure to equilibrate. 

Select ODOsat % and then 1-Point to access the DO calibration procedure. Calibration of 

your Optical dissolved oxygen sensor in the DO % procedure also results in calibration of 

the DO mg/L mode and vice versa. 

Enter the current barometric pressure in mm of Hg. (Inches of Hg x 25.4 = mm Hg). 

Note: Laboratory barometer readings are usually “true” (uncorrected) values of air 

pressure and can be used “as is” for oxygen calibration. Weather service readings are 

usually not “true”, i.e., they are corrected to sea level, and therefore cannot be used until 

they are “uncorrected”. An approximate formula for this “uncorrection” (where the BP 

readings MUST be in mm Hg) is: 

True BP = [Corrected BP] – [2.5 * (Local Altitude in ft above sea level/100)] 

Press Enter and the current values of all enabled sensors will appear on the screen and 

change with time as they stabilize. Observe the readings under ODOsat %. When they

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show no significant change for approximately 30 seconds, press Enter. The screen will 

indicate that the calibration has been accepted and prompt you to press Enter again to 

return to the Calibrate menu. 

As opposed to the 6562 Rapid Pulse Polarographic DO sensor (membrane) described 

above, there is no difference between the calibration routine for sensors which will be 

used for sampling or monitoring applications. Usually the Autosleep RS-232 feature in 

the Advanced|Setup menu will be activated for ROX calibrations, but there is no problem 

if it is not active. 

4. pH - using sufficient pH 7 buffer standard in a clean, dry or pre-rinsed calibration cup, 

carefully immerse the probe end of the sonde into the solution. Allow at least 1 minute 

for temperature equilibration before proceeding. From the Calibrate menu, select pH to 

access the pH calibration choices and then press 2-Point. 

Press Enter and input the value of the buffer (7 in this case) at the prompt. Press Enter 

again and the current values of all enabled sensors will appear on the screen and change 

with time as they stabilize in the solution. Observe the readings under pH and when they 

show no significant change for approximately 30 seconds, press Enter. The display will 

indicate that the calibration is accepted. After the pH 7 calibration is complete, press 

Enter again, as instructed on the screen, to continue. Rinse the sonde in water and dry the 

sonde before proceeding to the next step. 

Using an additional pH 10 buffer standard in a pre-rinsed calibration cup, carefully 

immerse the probe end of the sonde into the solution. Allow at least 1 minute for 

temperature equilibration before proceeding. Press Enter and input the value of the 

second buffer at the prompt. Press Enter and the current values of all enabled sensors 

will appear on the screen and will change with time as they stabilize in the solution. 

Observe the readings under pH and when they show no significant change for 

approximately 30 seconds, press Enter. After the second calibration point is complete, 

press Enter again, as instructed on the screen, to return to the Calibrate menu. 

The majority of waters sampled in conjunction with the Tampa Bay HBMP should be 

expected to have pH values between 7 and 10. Therefore, unless it is anticipated (or 

observed) that pH values of less than 7 occur, a two-point calibration using pH 7 and pH 

10 buffers is sufficient. 

Under unusual conditions where low pH values are expected, select the 3-point option to 

calibrate the pH probe using three calibration solutions. Using this procedure, the pH 

sensor is calibrated with a pH 7 buffer and two additional buffers (one higher and one 

lower). The 3-point calibration method assures maximum pH accuracy over a wider 

range of potential observations. The 3-point procedure is the same as that for a 2-point 

calibration, except that the software will prompt the selection of a third pH buffer to 

complete the 3-point procedure.

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5. Depth Sensor – Make certain that the depth sensor module is in air and not immersed 

in any solution. From the Calibrate menu, select Pressure-Abs (or Pressure-Gage if you 

have a vented level sensor) to access the depth calibration procedure. Input 0.00 or some 

known sensor offset in feet. Press Enter and monitor the stabilization of the depth 

readings with time. When no significant change occurs for approximately 30 seconds, 

press Enter to confirm the calibration. This zeros the sensor with regard to current 

barometric pressure. Then press Enter again to return to the Calibrate menu. For best 

performance of depth measurements, users should ensure that the sonde’s orientation 

remains constant while taking readings. This is especially important for vented level 

measurements and for sondes with side mounted pressure sensors. 

6. Calibration Record – Calibration constants can be viewed by accessing the path 

Advanced|Cal Constants (from the Main sonde menu). Additionally, when any sensor is 

calibrated, YSI series-6 sondes automatically create a file in sonde memory that provides 

details of the calibration coefficients before and after the calibration. The file will have a 

.glp extension and will have the Circuit Board Serial # as the default filename. The file 

can be viewed by following the path File|Directory|File Details|View from the Main 

sonde menu. Pressing 1-View file will show the calibration record for the sonde, as 

shown below: 

Calibration records for all sensors will automatically be stored in the .glp file until the 

Delete All Files command is used from the File menu. However, if the Delete command 

is issued, all files, including the .glp (calibration record) file will be lost. Therefore, it is 

extremely important to remember to upload the .glp prior to deleting files from the sonde.

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7. Preventative Maintenance - The following briefly summarizes key items regarding 

continuing preventive maintenance of YSI series-6 sondes. Further detailed information 

is available from the YSI manual or the associated web site. 

a. O-Ring Care - Series-6 sondes utilize user-accessible o-rings as seals to 

prevent water from entering the battery compartment and the sensor ports. The 

following procedures will prevent water from entering the sonde. If the o-rings 

and sealing surfaces on the sondes are not maintained properly, it is possible that 

water can enter the battery compartment and/or sensor ports. When the battery 

compartment lid is removed from battery version sondes, the o-rings that provide 

the seal should be carefully inspected for contamination and cleaned if necessary. 

The same inspection should be made of the o-rings associated with the probes, 

port plugs, and field cable connectors when they are removed. If no dirt or 

damage to the o-rings is evident, then they should be lightly greased without 

removal from their groove. However, if there is any indication at all of damage, 

the o-ring should be replaced.

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Use a small, flat-bladed screwdriver or similar blunt-tipped tool to remove the o- 

ring from its groove. Check the o-ring and the groove for any excess grease or 

contamination. If contamination is evident, clean the o-ring and nearby plastic 

parts with lens cleaning tissue or equivalent lint-free cloth. Alcohol can be used to 

clean the plastic parts, but use only water and mild detergent on the o-ring itself. 

Also, inspect the o-rings for nicks and imperfections. Using alcohol on o-rings 

may cause a loss of elasticity and may promote cracking. Do not use a sharp 

object to remove the o-rings. Damage to the o-ring or the groove itself may result. 

Before re-installing the o-rings, make sure that you are using a clean workspace, 

clean hands and are avoiding contact with anything that may leave fibers on the o- 

ring or grooves. Even a very small bit of contamination can cause a leak. 

To re-install the o-rings, place a small amount of Teflon stopcock grease between 

your thumb and index finger. (More grease is NOT BETTER!) Draw the o-ring 

through the grease while pressing the fingers together. Use this action to place a 

VERY LIGHT covering of grease to all sides of the o-ring. Place the o-ring into 

its groove making sure that it does not twist or roll. Use the previously greasecoated 

finger to once again lightly go over the mating surface of the o-ring. DO 

NOT apply excess grease on the o-ring or the o-ring groove. The excess grease 

may collect grit particles that can compromise the seal. Excess grease can also 

cause the waterproofing capabilities of the o-ring to diminish, potentially causing 

leaks into the compartment. If excess grease is present, remove it using lens cloth 

or lint-free cloth. 

b. Sonde Probe Ports - Whenever installing, removing or replacing a probe, it is 

extremely important that the entire sonde and all probes be thoroughly dried. This 

is extremely important to prevent water from entering the sonde through the port. 

Once you remove a probe or plug, examine the connector inside the sonde probe 

port. If any moisture is present, use compressed air to completely dry the 

connector. If the connector is corroded, return the sonde to YSI Customer Service. 

c. Cable Connector Port - The cable connector port at the top of the sonde 

should be covered at all times. While communicating with the sonde, a cable 

should be installed and tightened in place. This will assure that a proper 

connection is being made and prevent moisture and contaminants from entering. 

When a communications cable is not connected to the cable connector port, the 

pressure cap supplied with the instrument should be securely tightened in place. If 

moisture has entered the connector, dry the connector completely using 

compressed air, a clean cloth, or paper towel. Apply a very thin coat of lubricant 

to the O-ring inside the connector cap before each installation. 

d. Rapid Pulse DO Probes (with membranes) - Once the probes have been 

properly installed, remember that periodic cleaning and DO membrane changes 

are required. For best results, the KCl solution and the Teflon membrane at the tip

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of the DO probe should be changed prior to each extended sonde deployment 

and/or at least once every 30 days during the use of the sonde in sampling studies. 

In addition, the KCl solution and membrane should be changed if: 

• Bubbles are visible under the membrane. 

• Significant deposits of dried electrolyte are visible on the membrane or the O- 

ring. 

• The probe shows unstable readings or other probe-related symptoms. 

After removing the used membrane from the tip of the DO probe, examine the 

electrodes at the tip of the probe. If either or both of the silver electrodes are black 

in color, the probe should be resurfaced using the fine sanding disks provided by 

YSI in the reconditioning kit. To resurface the probe using the fine sanding disk, 

follow the instructions below. 

First dry the probe tip completely with lens cleaning tissue. Next, hold the probe 

in a vertical position, place one of the sanding disks under your thumb, and stroke 

the probe face in a direction parallel to the gold electrode (located between the 

two silver electrodes). The motion is similar to that used in striking a match. 

Usually 10-15 strokes of the sanding disk are sufficient to remove black deposits 

on the silver electrodes. However, in extreme cases, more sanding may be 

required to regenerate the original silver surface. After completing the sanding 

procedure, repeatedly rinse the probe face with clean water and wipe with lens 

cleaning tissue to remove any grit left by the sanding disk. After cleaning, 

thoroughly rinse the entire tip of the probe with distilled or de-ionized water and 

install a new membrane. 

It is extremely important to: 

• Use only the fine sanding disks provided in the maintenance kit.

Page 33 

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April 2008 


• Sand in a direction parallel to the gold electrode. 

Not following either of these instructions can seriously damage the electrodes. If 

this procedure is unsuccessful, as indicated by improper probe performance, it 

may be necessary to return the probe to the YSI service center. 

e. Conductivity/Temperature Probes – The openings that allow fluid access to 

the conductivity electrodes must be cleaned regularly. The small cleaning brush 

included in the YSI Maintenance Kit is ideal for this purpose. Dip the brush in 

clean water and insert it into each hole 15-20 times. In the event that deposits 

have formed on the electrodes, it may be necessary to use a mild detergent with 

the brush. After cleaning, check the response and accuracy of the conductivity cell 

with a calibration standard. If this procedure is unsuccessful, or if probe 

performance is impaired, it may be necessary to return the probe for service. 

f. Depth Sensor - The depth sensor modules are factory installed options that are 

located between the bulkhead and the sonde tube. For 600XL and 600XLM 

sondes, there is a circular protective cap with two small holes. The cap can not be 

removed, but a syringe is supplied in the maintenance kit to aid in cleaning the 

pressure port. Fill the syringe with clean water, place the tip of the syringe into 

one of the holes and gently force water through the pressure port. Ensure that the 

water comes out of the other hole. Continue flushing the pressure port until the 

water comes out clean. Never try to remove the circular pressure port cap. For 

6920, 6600 and 6820 sondes, the depth sensor is exposed to the water by either a 

circular access port on the side of the sonde or a through-hole on a module just 

above the sonde bulkhead. A syringe is supplied in the maintenance kit to aid 

cleaning the pressure port. Fill the syringe with clean water, place the tip of the 

syringe into one of the holes and gently force water through the access port. 

Ensure that the water comes out of the other hole. Continue flushing the pressure 

port until the water comes out clean. Do not attempt to remove the depth module 

from the sonde body. 

5.16 Hydrology - Permanent Station Continuous Recorders 

5.16.1 Introduction 

The TBC/Alafia HBMP employs permanently deployed water quality and water level 

monitoring equipment at six sites within the study area (Table 5.8). The objectives of 

these “continuous recorder”/permanent stations are to: 

• Estimate the daily freshwater inflows and water levels in each reporting 

unit.

Page 34 

Section 5.0 

April 2008 


• Estimate the trend of water quality indicators by reporting unit on an 

appropriate temporal basis. 

The permanently deployed equipment records specific conductance and/or water levels at 

15-minute intervals. A different array of sensors is deployed at each site in accordance 

with recommendations of the Focus Group. The specific sensors deployed at each site 

are listed in Table 5.8. 

5.16.2 Continuous Recorders 

There is one continuous recorder site each in the Alafia River, Palm River (Tampa 

Bypass Canal), and McKay Bay Reporting Units. The Alafia site is located on a piling 

about a kilometer below Buckhorn Springs. The Palm River site is on the bridge fenders 

for Maydell Drive. The McKay Bay site is located on one of the pilings for the 22 nd 

Street Causeway at the mouth of McKay Bay. The remaining three sites are in the 

Hillsborough River Reporting Unit. The Crosstown Expressway site is located on one of 

the bridge pilings. The Columbus Avenue site is located on the bridge fender. The Sligh 

Avenue site is attached to one of the pilings that protect the Sligh Avenue Bridge. 

The permanent stations are comprised of seven main pieces of equipment: 

• Campbell Scientific CR10X Measurement and Control Modules or Stevens Data 

Online Telemetry (DOT) Loggers 

• YSI 600R Sondes, 

• Stevens SDI-12 Shaft Encoders (at select sites), 

• Solar voltaic panels, 

• Batteries, 

• Stilling wells, and 

• Electronics boxes. 

Specifications for the sensors on these pieces of equipment are listed in Table 5.9. 

Several users and operations manuals for these pieces of equipment are referenced below. 

A copy of each of these manuals is maintained in the PBS&J Tampa office. 

Specific conductance and temperature are recorded at all sites. Salinity is calculated from 

specific conductance and temperature. Water level is also measured at two sites. 

The specific conductance sensor is deployed in a single stilling well. The water level 

float is deployed in a second stilling well. All equipment is powered by a battery charged 

by the solar voltaic panel. Each Shaft encoders also has a backup battery. 

Specific conductance is measured at two fixed elevations, nominally called “surface” and 

“bottom.” The bottom measurement is a good approximation of the water quality 

conditions near the bottom of the water column. The surface measurements are

Page 35 

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April 2008 


indicative of the conditions in the upper part of the water column, but not necessarily of 

conditions at the surface of the water column. The surface probes are occasionally 

exposed during very low tides. The data collected by exposed probes should be given 

invalid data qualifiers. 

5.16.3 Downloading, Calibration, and Maintenance 

Standard operating procedures for continuously deployed water quality monitoring 

equipment require that calibration and maintenance logs be kept. The basic download, 

calibration, and maintenance procedure consists of five major steps: 

1. Perform a profile cast. 

2. Download Campbell data recorder (data on Stevens data loggers are downloaded 

by Tampa Bay Water staff. 

3. Maintain sensors, probes, recorders, stilling wells, and power systems. 

4. Check calibration of sensors, probes, and level recorders. 

5. Recalibrate equipment as necessary. 

These steps can be preformed in any order. Whenever time allows, a profile cast should 

be preformed. These casts are used to characterize conditions across the entire water 

column, relative to those at the levels of the sensors. 

5.16.4 Electronics Box, Solar Voltaic Panels, and Batteries. 

Each site contains at least one electronics box, one solar panel, and one battery. The type 

and model of solar voltaic panel used on each station varies. Some stations have two 

panels. The panel charges the internal battery that powers the data logger and YSI 

sondes. A separate battery that must be replaced periodically powers the shaft encoder. 

Panel maintenance consists of wiping off the panel surface and making certain that the 

connections and wiring are in good condition and functional. 

The electronics should be checked and maintained once every two to four weeks. The 

electronics maintenance procedure consists of six steps 

1. Clean solar panel. 

2. Open box. 

3. Check wiring. 

4. Replace stage recorder battery if present and if necessary. 

5. Close and lock box once downloading and other maintenance are complete.

Page 36 

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5.16.5 Data Loggers 

Campbell Scientific CR10X Measurement and Control Module 

CR10X Measurement and Control Modules are the primary datalogger/controller for the 

continuous recorders. The manufacturer may be reached by phone at 435-753-2342 or on 

the Internet at www.campbellsci.com. 

The CR10X Modules are running software that should not be modified by field crews 

without consultation from the project manager. Software formulation is beyond the 

scope of this document. The CR10X Module is fully described in the CR10X 

Measurement and Control Module Operator’s Manual (Campbell Scientific, 1997). 

LoggerNet Data Logger Support Software is used to download data from the CR10X. 

The software is described in the LoggerNet Version 3.2 Instruction Manual (Campbell 

Scientific, Inc, December, 2005). This manual is included as an appendix at the end of 

this document. 

The CRX10 should be maintained and downloaded once every two to four weeks. The 

download procedure is as follows: 

CRX10 DOWNLOAD PROCEDURE 

1. Connect serial cable (SC929) between battery operated laptop & CR10X. 

2. Open LoggerNet. 

3. Click on CONNECT button (Figure 5.2a). 

4. Choose station from list on left side of screen (Figure 5.2b). 

5. “Connected” message should appear on right bottom corner of screen. 

6. Click NUMERIC button to check probe status (Figure 5.2b). 

7. Click COLLECT button to download data since last download or COLLECT 

ALL to collect all data stored (Figure 5.2b). 

8. Manual Data Collection indicator will show percent complete. 

9. If necessary, proceed to calibration procedures below. 

10. Check all values to ensure that all sensors are reading. 

11. Click DISCONNECT button. 

Stevens Data Online Telemetry (DOT) Loggers 

Stevens Water Quality Monitoring Systems data loggers have been utilized for at least 

one water quality monitoring station in place of a CR10X. The manufacturer may be 

reached by phone at 1-800-452-5272 or on the Internet at www.stevenswater.com.

Page 37 

Section 5.0 

April 2008 


The Stevens loggers have been programmed prior to deployment and should not be 

altered by field staff. Data recorded by Stevens data loggers are downloaded by Tampa 

Bay Water staff and transmitted to PBS&J office staff. Thus no maintenance or 

downloading of the Stevens data loggers is required by PBS&J staff. 

5.16.6 Specific Conductance (Conductivity) 

Specific conductance (conductivity) measurements are made using YSI 600R Sondes 

(Figure 5.3). YSI technical support may be reached by phone at 800 897-4151 or on the 

Internet at www.ysi.com. 

The operation and maintenance procedures for the 600R Sondes are described in the 6- 

Series YSI Environmental Operations Manual (YSI, 1999). The sondes are powered by 

the main station battery. Even when sondes are attached to external devices such as a 

laptop computer, they must be powered by an external battery. 

The sondes should be maintained and have their calibrations checked and be recalibrated 

once every two to four weeks. Sonde check-calibration and maintenance requires the 

following materials: 

• Laptop computer and software. 

• 9-pin serial cable. 

• YSI Sonde calibration cup. 

• Non-metallic brush (toothbrush or similar). 

• Water. 

• Conductivity standards (two standards that bracket the expected range of in situ 

values). 

• Calibration and Maintenance Log. 

The sonde check-calibration and maintenance procedure consists of nine steps. 

CONDUCTIVITY CHECK CALIBRATION & MAINTENANCE PROCEDURE 

1. Remove sondes from stilling well. 

2. Inspect sondes and wiring for damage. 

3. Disconnect sonde from CR10X. 

4. Attach sonde to computer. 

5. Check sonde for calibration using both high and low standards. 

6. Clean sondes. 

7. Recalibrate sondes using YSI sonde calibration procedure outlined above 

(recalibrate utilizing the higher conductivity standard and check with lower 

standard). 

8. Reattach sonde to CR10X. 

9. Place sondes in stilling well.

Page 38 

Section 5.0 

April 2008 


The sondes are calibrated using a YSI 650 or ECOWATCH FOR WINDOWS software 

and a laptop computer (connected to power source), conductivity standard, and a YSI 

calibration cup. Calibration procedures essentially follow those outlined for the YSI 

earlier in this chapter. 

5.16.7 Water Levels 

Water level readings are measured by a Stevens SDI-12 Shaft Encoder. The 

manufacturer may be contacted by phone at 800-452-5272. 

The Stevens SDI Encoder is a float-operating level sensing device that outputs data to the 

CRX10. The encoder measures water level by recording the level of a float suspended 

from a punched tape or beaded wire. A counter weight is suspended from the other side 

of the tape. The punched tape runs over a pulley, and the encoder records the movement 

of the pulley. 

The Stevens Recorder (Figure 5.4) is equipped with an LCD display, and can be fully 

programmed by the user without the use of any external device or computer. The LCD 

display can be used for device setup utilizing two push buttons. The display can also be 

set to continuously output the current level reading. Operation of the SDI Encoder is 

described in the SDI Encoder Instruction 91196 (Stevens Water Monitoring Systems, 

Inc., 1999). 

Maintenance and calibration of the water level recorder requires: 

• Tape measurer. 

• 9-volt alkaline battery. 

An LCD display is located on the top of the encoder. This is used to calibrate the 

encoder. One side of the encoder has three buttons. Two buttons, the left and right 

buttons, are set closely together. The third button is separated from the other two. This 

third button is the voltage display button. The LCD displays the options listed in Table 

5.10. These options are selected using the right and left buttons as indicated in Table 

5.10. 

The encoder has a backup 9-volt alkaline battery. The backup battery voltage can be read 

by pressing the button on the side of the unit near the LCD display. The battery should 

be replaced when the reading is below 8 volts. 

The encoder maintenance and calibration procedure is as follows: 

1. Inspect the stilling well to ensure that the float moves freely and without 

obstructions. 

2. Inspect float tape to ensure that it moves correctly on pulley. 

3. Clean the float if fouling influences its movement.

Page 39 

Section 5.0 

April 2008 


4. Clean or replace stilling well if fouling influences float movement. 

5. Clean, repair, and/or replace tape if fouling influences float movement. 

6. Record encoder reading. 

7. Drop tape into stilling well to measure current water level relative to datum. 

8. Record current water level. 

9. Set encoder to current water level using the ADJUST function 

i. To enter programming mode, press both right and left buttons for 

approximately 2 seconds. 

ii. The LCD should display “ADJUST”. 

iii. Press right button to decrease LCD reading to correspond to water level OR 

press left button to increase LCD reading to correspond to water level. 

iv. Press both buttons until LCD displays “Good”. 

v. Press left button to exit programming. 

10. Press battery voltage key to check battery voltage 

11. Record battery voltage. 

12. Replace battery if voltage is below 8 volts.

Page 40 

Section 5.0 

April 2008 


Table 5.1 

HBMP Water Quality Sampling Study Elements. 

Study 

Element 


Month 

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 

Total 

Hillsborough River 


Quality 

6 strata 

1 fixed bay 

station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

1/stratum 

1/station 

72 

12 

Alafia River 


Quality 


1 fixed bay 

station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

2/stratum 

1/station 

144 

12 

1 freshwater 

stratum (AR7) 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

2/stratum 

3/stratum 

30 

McKay Bay 


Quality 


Quality 

34 cells 3 4 3 4 3 3 10 10 10 3 4 3 60 


strata with 

Deep (D) 

channel / shallow 

(S) substrata 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

TBC/Palm River 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

2 D & 

2 S / 

stratum 

2 D & 

2 S / 

stratum 

2 D & 

2 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

1 D & 

1 S / 

stratum 

90 

1 fixed station on 

upstream side of 

S-160 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

12

Page 41 

Section 5.0 

April 2008 


Table 5.2 


Hydrology/Water Quality Indicators, Units Of Measure, Sources of 

Variability, and Potential Effects of Surface Water Withdrawals 

Units of 

Measure 

Sources of Variability 

Stream Flow cubic feet/second rainfall, runoff coefficient, 

groundwater seepage 

Potential Effects of 

Withdrawals 

decreased streamflows 

Water Level m tide stage, freshwater inflow decreased surface water 

elevations in upper reaches 

Salinity psu freshwater inflow, rainfall, tide stage, 

currents, wind 

Specific Conductance μmhos/cm freshwater inflow, rainfall, tide stage, 

currents, wind 

Temperature °C freshwater inflow, rainfall, depth, solar 

radiation, air temperature 

pH standard pH units freshwater inflow, rainfall, diel 

photosynthesis and respiration 

Dissolved Oxygen mg/L freshwater inflow, rainfall, diel 

photosynthesis and respiration, wind, 

current, sediment oxygen demand 

Secchi Disk Depth m freshwater inflow, suspended solids, 

color, solar radiation 

Chlorophyll-a μg/L freshwater inflow, nutrients, trace 

elements, solar radiation, light 

transmission 

Color 

Total Suspended 

Solids 

Cobalt Platinum 

Units 

mg/L 

freshwater inflow 

freshwater inflow, sediment grain size, 

velocity 

increased salinity 

increased specific conductance 

increased or decreased 

temperatures 

increased pH values 


dissolved oxygen 

concentrations 

increased or decreased Secchi 

disk depth 


chlorophyll-a concentrations 

decreased color 

increased or decreased TSS 

concentrations 

Orthophosphate* mg/L freshwater inflow, algal production decreased orthophosphate 

Total Phosphorus* mg/L freshwater inflow, algal production decreased total phosphorus 

Ammonia 

Nitrogen* 

mg/L 

freshwater inflow, algal production 

decreased ammonia 

Ammonium NH4* mg/L freshwater inflow, algal production decreased ammonium 

Nitrate-Nitrite 

freshwater inflow, algal production decreased nitrogen 

mg/L 

Nitrogen* 

Total Kjeldahl 

freshwater inflow, algal production 

decreased nitrogen 

mg/L 

Nitrogen* 

Total Nitrogen* mg/L freshwater inflow, algal production 

decreased nitrogen 

*Nutrients are not required by the original HBMP design. These parameters have been 

collected, and may be again in the future, for non-HBMP Tampa Bay Water projects.

Page 42 

Section 5.0 

April 2008 


Table 5.3 

Lower Hillsborough 

River 

Selected Critical Hydrologic and Water Quality Indicators Specified for 

each Waterbody 

Lower Palm River 

Tampa Bypass Canal 


Lower Alafia River 

stream flow 

stream flow 

stream flow 

stream flow 

surface water 

surface water 

surface water 

surface water 

elevation 

elevation 

elevation 

elevation 

salinity 

salinity 

salinity 

salinity 

specific conductance 




temperature 

temperature 

temperature 

temperature 

pH 

pH 

pH 

pH 





chlorophyll a 

chlorophyll a 

chlorophyll a 

chlorophyll a 

---------------- 

color 

color 

color 

orthophosphorus* 

Orthophosphorus* 



total phosphorus* 




ammonia/ammonium * 



ammonia/ammonium* 

nitrate+nitrite* 




total Kjeldahl nitrogen* 




total nitrogen* 




---------------- 

---------------- 

---------------- 

total suspended solids 

*Sampling for these parameters is not specified or required in the original or the current HBMP 

design. These parameters have been collected during certain water years for other Tampa Bay 

Water projects.

Table 5.4 Standard Formats & Information Requirements for Field Notebooks 

Notebook #: Project: _________________ Task: Page #: ________________ 

Date: ____________________________ (yy/mm/dd) 

Hydrolab/YSI Unit #: __________________ 

Air Temperature: __________________________ Wind: ______________________________ 

Tide Stage: ______________________________ Cloud Cover: ________________________ 

Reporting Unit 

Geo Stratum: _____________________ Temp- Specific Dissolved 

erature Cond. Salinity pH Oxygen 

Time On 

Depth (m) ( C) ( mS/cm) ( ppt) (mg/L) 

Station _______________ 0.2 _________ _________ __________ _______ ________ 

24 hr. / EST 

0.5 _________ _________ __________ _______ ________ 

Stratum/ _______________ 1.0 _________ _________ __________ _______ ________ 

1.5 _________ _________ __________ _______ ________ 

Station # _______________ 2.0 _________ _________ __________ _______ ________ 

2.5 _________ _________ __________ _______ ________ 

Lateral Position _________ 3.0 _________ _________ __________ _______ ________ 

3.5 _________ _________ __________ _______ ________ 

Secchi depth _____________ 4.0 _________ _________ __________ _______ ________ 

(meters) 

4.5 _________ _________ __________ _______ ________ 

Secchi @ bottom Yes 5.0 _________ _________ __________ _______ ________ 

5.5 _________ _________ __________ _______ ________ 

Water depth _____________ 6.0 _________ _________ __________ _______ ________ 

(meters) 

(water column depth) 6.5 _________ _________ __________ _______ ________ 

Latitude 

Longitude 

Degrees Decimal Minutes Degrees Decimal Minutes 

Projected: ___________ ___________________ 82 __________________ 

Actual: ___________ ___________________ 82 __________________ 

Samples: Check Container Numbers Check Custody Forms 

Sample Taken Samples Processed Samples Preserved Sediment Sample Taken 

(check): 

Field Notes: __________________________________________________________________________________ 

____________________________________________________________________________________________ 

____________________________________________________________________________________________ 

____________________________________________________________________________________________ 

____________________________________________________________________________________________ 

____________________________________________________________________________________________ 

_______________ ______________ _____________ ______________ 

____________________________________________________________________________________________ 

Signed Date Signed Date 

_______________ 

Copied- Initial & date 

________________ 

Entered- Initial & date

Page 44 

Section 5.0 

April 2008 


Table 5.5 

Required Containers, Preservation, Holding Times and 

Sample Volumes 

Parameter 

Container 

Type 

Preservation 

Holding Time 

Chlorophyll a 

1 L Amber 

Glass 

(Filter) 

Cool Dark; if filtered 

freeze until extraction. 

48 hours (Low 

light 

environment) 

15 days frozen 

filter 

Color 1 L Plastic¹ Cool 4°C 48 hrs 

Conductivity 1 L Plastic¹ Cool 4°C 28 days 

TSS 1 L Plastic¹ Cool 4°C 7 days 

Orthophosphate Phosphorus 1 L Plastic¹ Cool 4°C 48 hours 

Total Phosphorus 

250 mL Plastic² 

H 2 SO 4 pH

Page 45 

Section 5.0 

April 2008 


Table 5.6 In-situ YSI Water Quality Indicators, Units Of Measure, Accuracy, 

Resolution and Range 

Indicator Units Accuracy Resolution Range 

Temperature °C +/- 0.01 °C 0.01 °C -5 °C to 50 °C 

Specific Conductance µmhos/cm +/- 1% of reading 

+/- 1 µmhos/cm 

four digits 0 to 10000 

µmhos/cm 

Salinity ppt +/- 0.2 ppt 0.01 ppt 0 to 70 ppt 

pH standard pH units +/- 0.2 units 0.01 units 0 to 14 units 

Dissolved Oxygen mg/L +/- 0.2 mg/L 0.01 mg/L 0 to 20 mg/L 

Depth m +/- 0.08 m 0.01 m 0 to 25 m

Page 46 

Section 5.0 

April 2008 


Table 5.7 Freshwater Temperature (°C) Versus Dissolved 0xygen (mg/L) Saturation at 

760 mm hg 

Temperature 


19.00 9.24 

19.25 9.20 

19.50 9.15 

19.75 9.10 

20.00 9.06 

20.25 9.02 

20.50 8.97 

20.75 8.92 

21.00 8.88 

21.25 8.84 

21.50 8.80 

21.75 8.75 

22.00 8.71 

22.25 8.67 

22.50 8.63 

22.75 8.59 

23.00 8.55 

23.25 8.51 

23.50 8.47 

23.75 8.43 

24.00 8.39 

24.25 8.35 

24.50 8.31 

24.75 8.28 

25.00 8.24

Page 47 

Section 5.0 

April 2008 



Table 5.8 HBMP Continuous Recorder Locations and Sensors 

Site 


Level 

Specific 

Conductance 

Temperature 

Alafia Buckhorn Springs X X 

Palm Maydell Dr. X X 

McKay 22 nd St. Causeway X X X 

Hillsborough Crosstown Expressway X X X 

Hillsborough Columbus Ave. X X 

Hillsborough Sligh Ave. X X 

Table 5.9 Specifications for the Sensors as Reported by Manufacturers 

Parameter Sensor Range Accuracy Resolution 

YSI 600R Sonde 

Conductivity 

4 electrode cell with 

autoranging 

0 to 100 

mS/cm 

Salinity none (calculated) 0 – 70 ppt 

+/- 0.5% of reading + 0.001 

mS/cm 

The greater of +/- 1% of 

reading or 0.1 ppt 

0.001 to 0.1 

mS/cm 

0.01 ppt 

Temperature Thermistor -5 to 45 C +/- 0.15 C 0.01 C 

Stevens SDI-12 Shaft Encoder 

Water Level 

Float 

-999.99 to 

999.99 

+/- 0.01 foot 0.001 m

Page 48 

Section 5.0 

April 2008 


Table 5.10 Stevens Encoder LCD Menu Options Summary 

Option Description Selection Button Actions 

ADJUST 

ADDRESS 

UNITS 

AVERAGE 

LCD 

Sets the current reading up or 

down 

Do Not Use for this Project 

Sets the measurement units 

Do Not Use for this Project 

Set low power mode. Either 

determined by switch, always 

ON, or auto OFF in 15 seconds. 

ZERO Zero shaft encoder CLEAR 

SCALE 

EEPROM 

VERSION 

Set pulley wheel scale factor. 

Two part process. First scale is 

entered. Then scale units are 

entered. 

Reset EEPROM to factory 

defaults 

Display firmware version 

-999.99 to 999.99 

“M” display 

meters; “F” display 

feet 

RIGHT 

LEFT 

Scroll up 

BOTH Enter 

5 seconds Exit 

RIGHT 

BOTH 

LEFT 

Scroll down 

Select M or F 

Enter 

Exit 

SWITCH RIGHT Select option 

ON BOTH Enter 

OFF LEFT Exit 

-999.99 to 999.99; 

“M” display 

meters; “F” display 

feet 

INIT 

BOTH 

RIGHT 

LEFT 

RIGHT 

LEFT 

BOTH 

Zero reading 

Exit 

Exit 

Scroll up 

Scroll down 

Enter 

5 seconds Leave unchanged 

BOTH 

RIGHT 

LEFT 

BOTH 

RIGHT 

LEFT 

Set defaults 

Exit 

Exit 

Exit 

Exit 

Exit

Page 49 

Section 5.0 

April 2008 


Figure 5.1 Organizational chart for hydrology and water quality HBMP elements

Page 50 

Section 5.0 

April 2008 


Figure 5.2a LoggerNet Startup screen showing CONNECT button 

Figure 5.2b LoggerNet Connect screen

Figure 5.3 YSI 600R sonde. Note perforated and weighted sonde guard 

at base of sonde. 

Page 51 

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Figure 5.4 Stevens SDI-12 Encoder. The control buttons are located 

on the far side. 

Page 52 

Section 5.0 

April 2008 


Appendix 5-A 

Forms

Sample chain of custody form

Equipment description: 

Equipment Repair Form 

Date reported: 

Signature: 

Problem description: 

Action taken: 

Repair description: 

Date resolved: 

Resolved by (signature): 

Project Manager (signature): 

QA/QC Check (signature):

Notebook # Project: _________________ Task: Page # _____ 

Date: _____________________________ (yy/mm/dd) 

Hydrolab/YSI Unit #: ___________________ 

Calibration Book Number:__________________ 

Calibration Book Page #:_________________ 

Air Temperature: ___________________________ Wind: ________________________________ 

Tide Stage: _______________________________ Cloud Cover: __________________________ 


Geo Stratum: ______________________ Temp- Specific Dissolved Check 

erature Cond. Salinity pH Oxygen If 

Depth (m) ( C) ( mS/cm) ( ppt) (mg/L) Bottom 

Time On 

Station ________________ 0.2 __________ __________ ___________ ________ _________ 

24 hr. / EST 

0.5 __________ __________ ___________ ________ _________ 

Stratum/ ________________ 1.0 __________ __________ ___________ ________ _________ 

1.5 __________ __________ ___________ ________ _________ 

Station # ________________ 2.0 __________ __________ ___________ ________ _________ 

2.5 __________ __________ ___________ ________ _________ 

Lateral Position __________ 3.0 __________ __________ ___________ ________ _________ 

3.5 __________ __________ ___________ ________ _________ 

Secchi depth ______________ 4.0 __________ __________ ___________ ________ _________ 

(meters) 

4.5 __________ __________ ___________ ________ _________ 

Secchi @ bottom Yes 5.0 __________ __________ ___________ ________ _________ 

5.5 __________ __________ ___________ ________ _________ 

Water depth ______________ 6.0 __________ __________ ___________ ________ _________ 

(meters) 

(water column depth) 6.5 __________ __________ ___________ ________ _________ 

Latitude 

Longitude 

Degrees Decimal Minutes Degrees Decimal Minutes 

Projected: ____________ ____________________ 82 ____________________ 

Actual: ____________ ____________________ 82 ____________________ 

Samples: Check Container Numbers Check Custody Forms 

Sample Taken Samples Processed Samples Preserved Sediment Sample Taken 

(check): 

Field Notes: ____________________________________________________________________________________ 

_______________________________________________________________________________________________ 

_______________________________________________________________________________________________ 

___________________ _________________ ________________ ________________ 

Signed Date Signed Date 

_______________ 

Copied- Initial & date 

________________ 

Entered- Initial & date

HYDROLAB/YSI CALIBRATION SHEET Book # Page # ______ 

Project _____________________________ 

Display Model ________________________ 

Sonde Model ________________________ 

Task ____________________________________ 

Display Name____________________________ 

Sonde Name _____________________________ 

Name of Calibrator ___________________ Date _______________ Time ___________ 

1. CONDUCTIVITY: 

a. Rinse twice with standard (use standards greater & less than expected field conditions) 

b. Fill cup with conductivity standard 

Standard mS/cm Standard Temp Before After Cal. Constant 

Hi 

Lo 

c. Rinse twice with D.I. water 

2. pH 

a. Replace pH junction & solution, if necessary 

Replaced by:_____________ Date:_____________ Time:_____________ 

b. Rinse twice with pH 7 standard 

c. Fill cup with pH 7 standard 

Standard Standard Temp Before After 

d. Rinse twice with D.I. water 

e. Rinse twice with pH 10 standard 

f. Fill cup with pH 10 standard 

Standard Standard Temp Before After 

g. Rinse twice with D.I. water 

h. Repeat steps b and c and check ph 7 again 

Standard Standard Temp Before After Cal. Constant 

i. Rinse twice with D.I. water 

3. DISSOLVED OXYGEN: 

a. Check DO membrane. 

b. Replace DO Electrolyte and membrane, if necessary 

Replaced by:_____________ Date:_____________ Time:_____________ 

c. Blot away water drops on membrane with lens tissue. 

Temp Bar. Press Before After DO Charge Cal. Constant 

d. Rinse twice with D.I. water 

___________________ 

Copied – Initial & Date 

___________________ 

Entered – Initial & Date

HYDROLAB/YSI CALIBRATION SHEET Book # Page # ______ 

4. DEPTH (at surface): Depth before ____________ Depth after __________________ 

5. BATTERY CHECK: Surface Unit Voltage ____________________ 

Were batteries charged/replaced? ____________________ 

6. Notes: ________________________________________________________________________ 

______________________________________________________________________________________ 

______________________________________________________________________________________ 

______________________________________________________________________________________ 

7.0 Status of Unit: Good Minor Repairs Major Repairs 

1) Sonde 0 0 0 

2) Surface Unit 0 0 0 

3) Cables 0 0 0 

8.0 CALIBRATION CHECKS 

4-Hour 8-Hour After Use 

Date 

Time 

Name of Calibrator 

Parameter 

4-Hour 

Solution 

4-Hour 

Solution 

Temp. 

4-Hour 

Check 

8-Hour 

Solution 

8-Hour 

Solution 

Temp. 

8-Hour 

Check 

After Use 

Solution 

After Use 

Solution 

Temp. 

After Use 

Check 

Conductivity (high) 

Conductivity (low) 

pH 


DO Charge 

Depth 

Does Unit Pass Post Calibration Check? Yes 0 No 0 * 

• Note: If the unit does not pass post calibration check then data can not be entered into the data 

base without approval of QA/QC Office and/or Project Manager. Also, call Lab and put hold on 

chemistry parameters with long holding times. 

__________________________ 

Signature and Date 

___________________ 

Copied – Initial & Date 

____________________________ 

Signature and Date 

___________________ 

Entered – Initial & Date

Appendix 5-B 

LoggerNet Datalogger 

Support Software Instruction Manual

Appendix 5-C 

ACT Performance Verification Statement for 

YSI Rapid Pulse Dissolved Oxygen Sensor

Page 1 

Section 6.0 

April 2008 



This section describes the protocols, methods, and procedures that comprise the benthic 

macroinvertebrate monitoring elements of the HBMP. It is imperative that the field and 

laboratory methods used by HBMP personnel correspond to those contained in this 

portion of the QA/QC Plan. Use of the standardized sampling procedures, sampling 

techniques, species identification, data recording, and other associated tasks will ensure 

that these data can then be appropriately analyzed with previously collected baseline data. 

Figure 6.1 provides an overview of the organizational management of this study element, 

while Table 6.1 summarizes both the number and frequency of samples collected 

annually in each of the sampling strata. 

Members of the team conducting these elements of the HBMP must read, understand and 

follow all of the steps in each procedure outlined below. If questions or suggestions 

arise, the Project Manager must be consulted for clarification before any further actions 

can be taken. Under no circumstance should a procedure or steps within a procedure be 

omitted or modified in the field. 

6.1 Field Sampling 

Field sampling will normally be conducted by at least two PBS&J staff whenever 

possible. Field staff will be trained in all sampling procedures and will have copies of all 

the pertinent chapters of the HBMP Project Specific QA/QC Document to consult in the 

field. 

6.1.1 Equipment 

The navigation and water quality profiling equipment (including GPS, multi-parameter 

sondes, Secchi disk, etc.) are described in Chapter 5. A checklist of all benthic-sampling 

equipment is provided in Table 6.2. 

A Young-modified Van Veen grab-sampler is used to collect benthic sediment samples. 

The device is composed of a bell-shaped frame surrounding a two-door, hinged sampling 

bucket. The Van-Veen grab-sampler is the inner clamshell-like mechanism (Figure 6.2). 

It samples an area of approximately 0.04 m 2 . It is a standard marine-sediment sampling 

device. The Young modification is the bell-shaped frame that surrounds the grab 

sampler. With the Young modifications, the gear is self-leveling and has two, hinged 

doors on its top surface to allow sediments to be removed (for grain-size-analysis) before 

the sample is sieved. 

The frame also stabilizes the bucket so that the angle and depth of penetration remains 

consistent between samples. Several options are available to assure consistent 

penetration into different sediment types. The frame is constructed to accept bar weights

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that can be used while sampling in hard substrates to allow for proper grab penetration. 

Standard dive-weights are the simplest to use, but any form of weight that will attach to 

the frame may be employed. When working in shallow seagrass beds, a crewmember can 

stand on the frame to provide the extra weight. This technique is often required in turtle 

grass (Thalassia) beds where the grass tubers are difficult to cut. 

When working in soft sediments, Plexiglas or plywood panels can be fastened to the 

bottom of the grab in order to prevent it from sinking too deep in the sediments. The gear 

may also be deployed without the frame in soft-sediment areas, but this is not the 

preferred option. 

A 500-µm Wildco sieve bucket (Figure 6.2) is used to sieve the benthic sediment sample 

and remove organisms from the surrounding sediment. A 500-µm Fisher sieve is also 

carried on board as backup and a supplement to the sieve bucket. 


Whenever possible, initial field mobilization and preparation should be completed by the 

end of the day before each sampling event, in order to reduce potential mistakes. 

Dissolved oxygen membranes should be checked and/or changed at least 24 hours prior 

to calibration in order to allow sufficient time for stabilization should the membrane need 

to be replaced. Multi-parameter sonde calibration, maintenance, and operation will 

follow the procedures outlined in Chapter 5. 

Before leaving for each field-sampling event, the field crew will check all Sample 

Custody Forms and Checklists to make sure that they have: 

• An adequate number of clean sampling containers for both benthos and sediment. 

The relevant station number and date will be marked on each container as it is filled. 

• An adequate amount of borax-buffered formaldehyde. The formaldehyde should be 

buffered by adding laundry-grade borax to the formaldehyde container until the borax 

no longer dissolves. The formaldehyde will be considered to be fully buffered when 

un-dissolved borax is present in the formaldehyde container. Rose Bengal dye should 

be added to the buffered formaldehyde at a rate of 0.5 grams/liter. 

• Formaldehyde is a flammable and corrosive material that is probably carcinogenic. 

Care should be taken to extinguish all flames, prevent all contact with skin, and avoid 

inhaling fumes when working with formaldehyde. 

• Appropriate field notebooks, sampling station list-sheets, waterproof pens, pencils, 

and markers placed in waterproof bags or containers. 

• Tide predictions for the sampling period should be taken from the GPS or obtained 

from another appropriate source. 

• All appropriate Sample Custody and Tracking Forms.

Page 3 

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• GPS loaded with sample-station coordinates. 

• Transferred sample-station list to the appropriate field notebook (an example of a 

page from the field notebook was presented in Table 5.4). The number and 

distribution of stations on the sample station list must match the number and 

distribution of stations in Table 6.3. If the two do not match, do not proceed with 

field work until the problem is corrected. 

The field staff must check to see that all needed gear is functional (see Benthic Sampling 

Checklist), and that all sampling devices have been cleaned as necessary. If possible, 

backup sampling gear should be included to preclude possible problems in the field. 

Check measurements of all depth marks on calibrated lines (sondes and Secchi disk) and 

repair any damaged marks. (Remember: all lines must be recalibrated after any repairs, 

such as after sending sonde cables back for replacement of connectors, or other problems 

with the cables). 

The boat should be fueled and clean, and all necessary safety gear packed. Boat batteries 

should be checked and placed on slow charge. All electrical (trim and tilt) and 

mechanical (steering) systems should be checked. Boat usage and maintenance logs 

should be checked and updated. 

6.1.3 Same Day Field Mobilization 

• Calibrate all multi-parameter sampling units and complete relevant calibration sheets. 

If possible, a backup unit should be taken, as well as a complete calibration kit and 

minor repair kit. 

• Field vehicle and boat should be filled with gas, and coolers filled with enough ice to 

transport sediment samples during the course of collection. 

• If the sample station coordinates have not been previously entered into the GPS, they 

should be entered now. 

6.1.4 Sampling 

An outline of the sampling procedure is given in Table 6.4. Several methods and 

procedures used in the benthic sampling program are identical to those used in the water 

quality program (Chapter 5). The water-column profiles collected during benthic 

sampling will be combined with those collected in the water quality, fish, and 

ichthyoplankton sampling programs and analyzed as a single data set. 

Locating the Sample-Station 

The sample stations will be located using the procedure described in Chapter 5. If the 

sampling site cannot be sampled due to some unforeseen difficulty, the field staff will

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follow the alternate-station procedure outlined in Chapter 5. Upon arrival at the sampling 

location: the boat is securely anchored from the bow, and the multi-parameter sonde is 

placed over the side to equilibrate. A pole should be used to confirm the presence of 

sediment at the station location. If the bottom is rock and there is no sediment present, do 

not deploy the multi-parameter sonde until sediment is located. 

Station and climatic data are recorded and a water-column profile is measured as 

specified in Chapter 5. If the current is such that the collection of a grab-sample will not 

influence the water column profile, the grab-sampler may be deployed while the multiparameter 

sonde is deployed. Otherwise, the grab-sampler should not be deployed until 

the water-column profile is complete. 

If the water-column profile is collected while the grab-sampler is deployed, the profile 

must be collected upstream of and on the opposite side of the boat from the deployed 

grab-sampler. Note: as in the water quality sampling procedure, water-column profile 

measurements are made at 0.5-meter intervals except in: 1) the deep ship channel in 

Alafia River segment one, and 2) the deep strata of the Palm River where they are 

collected at 1.0 meter intervals. 

As with the water quality procedure, profile duplicates must be collected at either a 5% 

frequency (once per 20 profiles) or once per reporting unit per day, whichever is greater. 

Multi-parameter sonde calibrations are also checked every four hours, and the values are 

recorded in the calibration field book. 

Procedure if Sediment or Substrate Sample Cannot Be Collected 

If the station can be sampled, but a sediment or substrate sample cannot be obtained from 

the location, field staff should let out 5 meters of anchor line and attempt to retrieve a 

sample. If a sample cannot be obtained there, the crew should let out another 5 meters of 

anchor line and attempt to retrieve a sample. If these two attempts fail, the crew should 

retrieve the anchor and proceed in the direction of the next station while using a pole to 

test the bottom for loose substrate. If loose sediment or substrate cannot be found within 

100 meters of the original station, the crew should proceed to the alternate station as 

described in Chapter 5. 

The Field Control Officer (FCO) should check all entries to the field notebook prior to 

the field crew leaving the sampling location. The FCO should also make sure that all 

recorded sample container numbers match those designated for each sampling location. 

Each member of the field crew should check all entries after completion of each sampling 

event, and sign each page of the field notebook. The date of review must be recorded if 

different from the date sampled. 

Sampling Process 

Sampling begins once the boat is anchored. In certain situations (particularly for reasons 

of safety when sampling in a navigation channel), the crew may opt not to anchor the

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boat. In these cases, the boat operator must make certain the boat is held on station so 

that the water-column profile, sediment grab, and GPS position are all taken in the same 

location. 

The field sampling procedures below are summarized in Table 6.4. 

1. Record the station coordinates, time, date, Secchi depth, and conditions on 

station in the field book as described in Chapter 5. 

2. Place the sieve bucket in the sieving tub. 

3. Secure the grab doors with the setscrews and cock the grab. 

4. Connect the grab-sampler to the davit-line (if necessary), lift the sampler, and 

swing it overboard. Thoroughly rinse and clean the inside of the grab with the 

wash down pump and a brush if necessary. 

5. Lower the grab sampler at a controlled rate. This will both prevent injuries 

among the crew and minimize wave disturbance effects on the sediment 

surface. 

6. When the grab has settled on the bottom, pull on the connecting line several 

times to ensure the grab’s trip mechanism has released. 

7. Retrieve the grab-sampler. Swing the davit to bring the sampler on board, and 

lower the sampler onto the tub directly above the sieve bucket. The sampler 

must be situated so that all material will fall directly into the sieve bucket when 

the grab is opened. If the grab cannot be situated properly, a large, plastic bag 

(instead of the sieve bucket) should be used as the receiving container. 

8. Open the hinged, top door and determine if the sampling deployment was a 

success. A successful sample will be relatively level, contain intact sediment 

over the entire area of the grab, and have a depth of at least 6.9 cm in the center 

of the grab. Samples that contain no sediments, partially filled grabs, or grabs 

with grossly slumped surfaces are unacceptable. Grabs with grossly slumped 

surfaces are assumed to have contacted the sediment surface at an angle and 

may contain a disproportionate percentage of immediate surficial sediments. 

Grabs completely filled to the top, where a significant amount of sediment has 

escaped from the hinged door are also unacceptable for the same reason. It may 

take several attempts to obtain acceptable samples. If necessary, two or more 

grabs may be combined to create a composite sample in order to have a volume 

equal to at least 70% of the grab volume. 

9. Collect a core sample of sediment from the grab and place it in a labeled, 

sediment sample-bag. A core sample is collected by opening one of the grab 

doors and taking a vertical sample of sediment with a syringe or a trowel. The

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sample should be representative of the entire contents of the grab (as opposed to 

the surface or bottom-most layers). 

10. Record notes on the condition of the sample as necessary in the field book. 

Notes should include sample consolidation, the presence of large objects, 

organisms, etc. 

11. Open the jaws of the grab-sampler and transfer the contents to the sieve bucket. 

It is essential that the entire contents of the grab are transferred to the bucket. If 

a significant amount of sediment is lost at any time, a new grab should be 

collected and the original grab discarded. Rinse the inside of the grab with a 

spray bottle or a GENTLE spray from the wash down pump making certain that 

all washings fall into the sieve bucket. Inspect the grab to see that all contents 

have been transferred to the sieve bucket. 

12. Partly submerge sieve-bucket in the water and agitate it to wash away the 

sediment from the organisms and other particles larger than 500 um screening. 

If the water is rough, the bucket should be sieved in the sieving tub to prevent 

the loss of sample material from wave action. If large clumps of mud or clay 

are present, carefully break them up by hand. Be sure to rinse the sediment 

from hands into the sieve. If vegetation, wood, or other objects are in the 

sieve gently wash them with a squirt bottle (do not try to dislodge attached 

organisms), record in field book, then discard vegetation or object. Be 

extremely careful not to lose any of the bucket contents over the top rim. If the 

multi-parameter sonde is in the water, sieving must occur downstream of the 

sonde, regardless of whether measurements are being recorded. 

13. After the majority of the sediment is sieved off, gently rinse the remaining 

sample to one edge of the sieve by partial immersion and/or a GENTLE water 

spray. Using either fingers or a spoon, transfer most of the sample to an 

appropriately labeled (date and station number) container. Rinse any of the 

sample that falls on the outside of the sample container during transfer back 

into the sieve. Rinse the remaining sediments on the sieve into the jar with a 

GENTLE water spray. The final wash down should take place over a bucket or 

basin so spilled material can be recovered. Carefully inspect the sieve to ensure 

that all organisms, especially polychaete worms, are removed. Fine forceps 

may be used to transfer organisms from the sieve to the container if necessary. 

14. In some cases, particularly deep areas in the Palm River with clay substrate, the 

entire sample may sieve through leaving no remaining material or organisms. 

In this case, make a detailed note in the field book describing the occurrence. 

Note the occurrence on the custody form as well. These are still adequately 

sampled stations. There is no need to sample an alternate station. 

15. Place an internal tag containing the station number and sample date into the 

container. Preserve the sample by filling the container with buffered, Rose

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Bengal-treated formaldehyde until the sample is submersed or the seawaterformaldehyde 

solution is 10% formaldehyde or more (whichever is greater). 

The strength of the formaldehyde solution needs to be known before it is added 

to the container. Assume that recycled formaldehyde is slightly stronger than 

10%. There should be adequate liquid to assure that the entire sample will 

always be submersed. 

16. Gently invert the container several times to mix the formaldehyde solution 

throughout the sample. 

17. Log the sieving and preservation in the field book and custody form, and place 

the container in a cooler or other secure place for transportation. 

18. Wash the Van-Veen sampler, lower the sampler to the deck, and secure the grab 

doors. Do not place the sampler on any sonde cables. Severe and/or 

potentially expensive damage may result. 

19. Set the GPS to navigate to the next station, and retrieve the sonde, anchor and 

other equipment. 

20. Proceed to the next sample station. 

Safety Considerations 

The Young-modified Van-Veen sampler is a large, heavy, and potentially dangerous 

piece of equipment. Once the device is cocked, it can release at any time. The operators 

must be careful not to place hands, fingers, feet, or other limbs and objects in a position 

where they could be caught if the device releases accidentally. Similarly, the operators 

must take precautions when deploying or retrieving the gear and at other times when the 

sampler is suspended above the deck. The sampler is heavy and has sufficient 

momentum when moving or swinging at the end of davit to injure or knock crew 

members overboard. 

Lost Equipment 

Losing gear might severely affect the efficiency, accuracy, and cost-basis of the sampling 

program. Crews should take every precaution against the loss of gear by properly 

tightening shackles and other connectors. If a piece of equipment is lost, attempts to 

recover it as described below should be followed. If the gear cannot be retrieved 

immediately, and a spare is present on board, the recovery effort should not extend past 

several hours. Attempts to recover gear are as follows. 

If the grab sampler or sonde are lost, immediately mark the area with the on-board buoy 

and record the GPS coordinates. Attempt to recover by grappling in the area where the 

sampler was dropped using an anchor or a grappling hook. If the gear is successfully 

recovered, continue with sampling. If it cannot be recovered, remain on station and

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contact the PBSJ office (813- 282-7275) to mobilize a diver. If you must leave the losslocation 

to pick up a diver, mark the location a second time with another buoy (if 

available). 

6.2 Sample Analyses 

6.2.1 Macroinvertebrate Analysis 

Terra Environmental Services Inc (Terra Environmental) has been contracted by PBS&J 

to conduct laboratory processing, data management, quality control, and project 

management associated with the sample sorting and taxonomic identification of the 

benthic monitoring component of the HBMP. As stipulated in the contract, Terra 

Environmental will maintain an up-to-date, project-specific Quality Assurance/Quality 

Control (QA/QC) Plan describing all procedures to be followed in the completion of the 

contracted work. The goals of the plan are to maximize the precision and accuracy of 

laboratory procedures, so as to obtain the most information possible from the sampling 

effort. The procedures to be employed are consistent with those utilized by previous 

laboratories contracted under the HBMP as stipulated in Version 1.1 of the HBMP 

QA/QC Plan (PBS&J 2002) and referenced in Florida Department of Environmental 

Protection SOP-002/01, LQ 7400, “Laboratory Quality Control for Macroinvertebrate 

Taxonomic Identification”. Species identification of benthic infauna and epifauna to the 

lowest practical taxonomic levels will be undertaken using the procedures and protocols 

outlined below (adapted from Terra Environmental’s QA/QC Plan for the Benthic 

Monitoring Component of the HBMP (Terra Environmental, 2007)). The resulting data 

will be used in assessing indicator measurements (Table 6.5) with respect to both 

seasonal and long-term variations in freshwater inflows. 

Sample Custody 

Upon receipt by Terra Environmental, all samples will be checked for leakage and 

inventoried. Custody forms provided by PBS&J will be signed by Terra Environmental 

staff and a sample log will be maintained. Terra Environmental staff will sign and date 

the sample log when samples are opened and sorted, and when taxonomic analysis is 

complete. 

Sorting 

Samples will remain in formalin (or buffered formaldehyde) for a minimum of 48 hours. 

After this minimum holding time, samples will be re-sieved (500-μm) and rinsed in the 

laboratory such that the formalin (formaldehyde), along with the “first flush” of rinse 

water will be collected in a hazardous waste storage container and returned to PBS&J for 

recycling. Terra Environmental staff will observe safety measures (wearing safety gear 

such as latex gloves, lab coats, and safety glasses; working in a ventilated area) while 

handling formalin (formaldehyde). All sample containers will be inspected to ensure all

Page 9 

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organisms have been removed and after resieving in the laboratory, sieves will be 

visually inspected and thoroughly rinsed with tap water between samples. 

The material remaining on the sieve will be placed (by aliquots if necessary) onto 18” x 

14” fiberglass sorting trays. Using an illuminated 5X magnifier, all organisms (stained 

pink by the Rose Bengal) will be carefully removed with forceps and placed into one of 

four labeled vials (Annelids, Mollusks, Arthropods, and Other) containing 70% ethanol. 

This process will be repeated until the entire sample is sorted. 

Sorting quality control will be achieved as follows. After a sample has been sorted 

(organisms separated as outlined above), remaining material will be placed back into a 

labeled container and held until a total of ten samples have been similarly sorted and 

stored. One of the ten sorted samples will be chosen randomly 

(http://www.randomizer.org/form.htm) for re-sorting by another analyst. Percent sorting 

efficiency will be calculated as: 

(Total # organisms) – ( #QC organisms) X 100 

(Total # organisms) 

where total # organisms = total number of organisms counted (sorted) in the sample, and 

#QC organisms = total number of organisms counted (sorted) in the QC check. 

An efficiency of 90% will be the minimum standard for sample sorting. If sorting 

efficiency of the QC sample is below 90%, the remaining nine samples also will be resorted. 

Documentation of sorting QA/QC will be made in the laboratory log book and on 

separate Sorting QA/QC forms (see Appendix 6-A). 

Taxonomic Identification 

Following sorting, all organisms will be enumerated and identified to the lowest practical 

taxonomic level using appropriate taxonomic keys, and all results will be recorded on 

laboratory bench sheets. 

When appropriate, oligochaetes and chironomids will be enumerated and identified as 

follows. If the number of organisms in either group exceeds 100, the entire sample will 

be placed into a gridded Petri dish. Organisms will be removed from randomly selected 

sections of the grid until 100 organisms have been obtained. These will be mounted on 

microscope slides and cleared for accurate identification using a compound microscope. 

The remaining organisms will be enumerated based on these results. 

Taxonomic identification quality control will be achieved as follows. The taxonomist 

will separate sorted organisms from each sample (as described above) by “species”. All 

individuals of a “species” (or closely related taxon) will be placed into a labeled sample

Page 10 

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vial filled with 70% ethanol. The sample number and “species” name will be included on 

each label. For each new “species” encountered, voucher specimens will be retained for 

confirmation by an outside expert a . After a particular identification has been confirmed 

by an outside expert, individuals of that species no longer need to be saved for 

verification when encountered in future samples. A list of confirmed species will thus be 

developed and maintained for each taxonomist. Representative individuals of each 

species confirmed by an outside expert will be retained in the Terra Environmental 

Invertebrate Reference Collection. Collection specimens will be stored in 70% ethanol 

with labels that include the taxonomic identification, station number, station location, and 

date collected. 

Organisms sorted and identified from all samples will be retained for at least three years. 

Data Management 

Data management includes data collection, documentation, handling, evaluation, 

verification, validation, and reporting. Raw data (taxonomic identification and number of 

individuals) are recorded on laboratory bench sheets that also include sample ID number, 

date of collection, location of collection, and substrate type (see Appendix 6-A). Data 

will then be entered into a computer database and a facsimile bench sheet will be printed 

out and used to verify (validate) data entry. All deliverable laboratory data then will be 

entered into tables (Excel or Access) provided by PBS&J. Terra Environmental will 

ensure proper quality control of the data prior to delivery of final datasets to PBS&J. In 

addition, Terra Environmental will develop and update an electronic taxonomic list and 

electronic data dictionary so that HBMP master-database users have access to benthic 

taxonomy data. Terra Environmental will be responsible for the following database 

deliverables: 

1. Phylogenetic taxonomic list 

2. Data dictionary 

3. Total number of individuals of each taxon in each sample 

Performance And Systems Audits 

Performance and/or systems audits will be performed as required. These audits will 

involve the QA/QC Officer and the Project Manager. 

Performance audits are conducted by reviewing project output regarding data accuracy, 

comparison of field and laboratory QA results, estimates of completeness, and 

recommendations for corrective actions. 

a Names of outside experts will be provided upon request

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Section 6.0 

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Systems audits involve a complete review of all planned tasks and will generally include 

a spot check of sample custody, adequacy of documentation, and overall data traceability. 

Corrective Action 

Any corrective action deemed necessary will be reported immediately, in writing, to the 

QA Officer. The potential need for corrective action could be identified through either 

internal or external quality assurance procedures during the course of conducting systems 

or performance audits. Alternatively, a potential need could be identified by field or 

laboratory personnel based on observation and work experience. If corrective action is 

deemed necessary, the details of implementing such action would be discussed and 

agreed upon by the QA Officer and Project Manager. 

6.2.2 Sediment Analysis 

PBS&J is responsible for the field collection of the sediment samples and for the delivery 

of the collected samples to Mote Marine Laboratory (Mote) for processing. Sediment 

samples collected during the benthic sampling efforts will be delivered to Mote monthly. 

Mote data quality standards include continuing calibration standards falling within 90- 

110% of known values, and precision targets with 20%RSD or less or within 2 detection 

limits. Mote utilizes data qualifier codes consisting of DEP-accepted designations, 

including “U” for less than the stated detection limit and PQL to indicate that the stated 

value is between the detection limit and a value four times greater. 

Samples are held frozen until analysis. Granulometry is performed using a laser 

diffraction instrument (Coulter, 1994) and the 93 size distribution channels consolidated 

into 26 individual channels corresponding with the Wentworth scale of half phi sizes and 

with standard %sand-silt-clay designations (Folk, 1974). Samples for grain size 

determination are first sieved of material greater than 2 mm, and a qualitative assessment 

is also made as to the amount of >2mm material present. Designations are 0-none, 1- 

minimal, 2-moderate, and 3-substantial. Percent organics are determined by ashing 

sediments at 500°C, Standard Methods 2540G, 18 th Edition (APHA,1992). 

Results of the percent organics and grain size analyses are provided in hard copy and 

electronically in an Excel format. Samples are disposed of 30 days from the time of data 

report delivery to PBS&J unless otherwise specified by PBS&J.

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Table 6.1 HBMP Benthic Macroinvertebrate Sampling Study Element 

Study 

Element 

Spatial 

Strata 

Month 

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 

Total 

Hillsborough River 

Benthos 6 strata 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 2/stratum 144* 

* Only samples collected from January through March, and July through September, will be processed. 


Benthos 34 Cells 3 4 3 4 3 3 10 10 10 3 4 3 60 

Alafia River 


2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

2/stratum 

144 

Benthos 

1 inset stratum 

(Rkm 7-13) 

7/stratum 

6/stratum 

7/stratum 

20 

1 freshwater 

stratum (AR7) 

3/stratum 

3stratum 

3stratum 

3/stratum 

3/stratum 

3/stratum 

3/stratum 

3/stratum 

3/stratum 

3/stratum 

1 SCI/ 

3/stratum 

3/stratum 

36 

1 SCI 

stratum 

Palm River 

Benthos 

3 strata with 

deep channel / 

shallow 

substrata 

PR1: 1D 

PR2: 1S 

PR3: 1S 

PR1: 1S 

PR2: 1D 

PR3: 1D 

PR1: 1S 

PR2: 1D 

PR3: 1S 

PR1: 1D 

PR2: 1S 

PR3: 1S 

PR1: 1S/1D 

PR2: 1S 

PR3: 1D 

PR1: 1D 

PR2: 1S/1D 

PR3: 1S/1D 

PR1: 2S/2D 

PR2: 2S/2D 

PR3: 1S/1D 

PR1: 1S/1D 

PR2: 2S/2D 

PR3: 2S/2D 

PR1: 2S/2D 

PR2: 1S/1D 

PR3: 2S/2D 

PR1: 1S 

PR2: 1D 

PR3: 1S 

PR1: 1D 

PR2: 1S 

PR3: 1D 

PR1: 1S 

PR2: 1D 

PR3: 1D 

60 

D=deep, S=shallow

Page 13 

Section 6.0 

April 2008 


Table 6.2 Benthic Sampling Checklist 

HBMP Benthic Sampling Field Checklist 

Field Gear 

Buoy & line 

Brushes (for cleaning gear) 

Cellular phone 

Clear tape 

Conductivity Solution (High and Low) 

Coolers 

Custody sheets 

Data field book 

Data field book, extra 

Davit 

De-ionized Water 

Downrigger 

Downrigger ball 

Duct tape 

Formaldehyde, w/ stain & buffered 

GPS preferably with stations entered 

GPS extra batteries (AA) 

Sonde Calibration Book 

Sonde display extra batteries (AA) 

Multi-parameter Sonde with Display Unit 

Leave In Office 

DO sensor Electrolyte Solution 

Station number & coordinate list 

Float plan 

Before Starting Sampling Effort (first day) 

Calibrate multi-parameter sonde 

Change DO membrane 

Charge hydrolabs 

Check COMPS system for observed tides 

Fill out custody forms 

Create station field sheet 

Load stations in GPS 

Make field notebook pages 

Complete field notebook pages 

Before Leaving for Field (second day) 

Disconnect chargers 

Calibrate multi-parameter sonde 

Get ice 

Ice 

Internal labels (tree tags) 

After Sampling 

Measuring tape 1. Complete custody forms 

Pens 2. Post-calibrate sonde(s) 

pH 10 Solution 3. Review & sign field notebooks 

pH 7 Solution 4. Photocopy field notebooks 

Quality Assurance Program Plan Manual 

5. Place sediment samples in freezer 

Sample bottle labels 

Sample bottles 

At Completion 

Sample bottles, extra 1. Deliver sediment samples 

Sample station field book with alternate list 2. Deliver benthic samples 

Secchi Disk 3. Send field notes to QAQC Officer 

Sediment bags 4. File field notes 

Sieve wash bucket, 500 um 5. Replenish supplies 

Sieve, 500 um 6. Store archived samples 

Squirt bottles 7. File custody forms 

Thermometer (generally attached to boat) 

Tide Predictions 

Trowel 

Weather forecast 

Young-modified Van Veen sampler

Page 14 

Section 6.0 

April 2008 


Table 6.3 Number of HBMP Benthic-Samples per Month by Reporting Unit 


Alafia Hillsborough Hillsborough McKay Palm Total 

Month 

Collected & 

Analyzed 

Collected & 

Analyzed 

Collected & 

Archived 

Collected & 

Analyzed 

Collected & 

Analyzed 

Collected 

January 15 12 3 3 33 

February 15 12 4 3 34 

March 15 12 3 3 33 

April 15 12 4 3 34 

May 15 12 3 4 34 

June 22 12 3 5 42 

July 21 12 10 10 53 

August 22 12 10 10 54 

September 15 12 10 10 47 

October 15 12 3 3 33 

November 15 12 4 3 34 

December 15 12 3 3 33 

Total 200 72 72 60 60 464 

Note: Hillsborough River samples are archived during the April through June and October 

through December sampling periods.

Page 15 

Section 6.0 

April 2008 


Table 6.4 Outline of Sampling Activities Performed at each Station 

1. Locate Station and anchor. 

2. Log station information, weather, tide, etc. 

3. Perform water column profile. 

4. Wash grab-sampler, if necessary, and check to see that doors are secure and grab is cocked. 

5. Lower grab-sampler, collect sample, and retrieve grab-sampler. 

6. Open grab-sampler lid and remove a core of sample for sediment analysis 

7. 

Label sediment-bag (date & station number) and transfer sediment core to bag. Place bag on ice 

in cooler. 

8. Open grab-sampler jaws and empty contents of sampler into sieve bucket. 

9. Sieve sample. 

10. Label appropriate size sample container (date & station number). 

11. Transfer sieved sample to container. 

12. Preserve sample with buffered formaldehyde. 

13. Place filled container in cooler or other transportation-storage location. 

14. Record sieving and preservation in field book, and record sample on custody forms. 

15. Wash, lower, and secure grab-sampler and secure doors. 

16. Move to next station. 

Table 6.5 Benthic Indicators, Units of Measure, and Sources of Variability 


Benthic Macroinvertebrate Epifauna & Infauna 

- Species Composition 

- Abundance 

- Distribution 

Units of 

Measure 

Species 

% composition 

# / unit area 

# / river km 

Sources of Variability 

depth, sediment grain size, 

sediment organic matter, 

dissolved oxygen, salinity, 

temperature, pH, submerged 

aquatic vegetation, season

Page 16 

Section 6.0 

April 2008 


Project 

Management 

Bob Woithe, Ph.D. 

Field Data 

Collection 

Field Staff 

Taxonomic 

Identification 

Terra Environmental 

Physical 

Water Column 

Measurements 

Benthic 

Macroinvertebrate 

Sampling 

Species 

Counts 

Data Preparation 

QA/QC 

Summary Tables 

Figure 6.1. Organization of the benthic macroinvertebrate study element.

Page 17 

Section 6.0 

April 2008 


A 

B 

C 

Figure 6.2 Basic benthic sampling equipment: A.) Van Veen grab; B.) Wildco wash 

bucket (500 um sieve); C.) Fisher sieves

Appendix 6-A 

Example Forms

Example Custody Form for Benthic Field Sampling 

December 2000 Tampa HBMP Benthic Sample Custody Form 

GPS Name Stratum Station Date Collected Date Delivered 

BA1 AR7 AR717737 

BA2 AR7 AR715643 

BA3 AR7 AR714246 

BA4 AR6 AR613930 

BA5 AR6 AR612909 

BA6 AR5 AR511615 

BA7 AR5 AR510502 

BA8 AR4 AR409054 

BA9 AR4 AR408054 

BA10 AR3 AR306212 

BA11 AR3 AR305910 

BA12 AR2 AR203655 

BA13 AR2 AR202845 

BA14 AR1 AR101682 

BA15 AR1 AR100783 

BH1 HR1 HR100397 Archived 












BM1 M16 M1600411 

BM2 M14 M1410806 

BM3 M21 M2104201 

BP1 PR1 PR102266 

BP2 PR2 PR205134 

BP3 PR3 PR306430 

Signatures: 

Delivered by: 

Received by: 

Date: 

Date:

Terra Environmental Sorting QA/QC Form 

Sample ID Date Sorted Sorted By No. Organisms QC Organisms Sorting Eff. Date Completed Completed By 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total 

worms 

shells 

bugs 

other 

total

Terra Environmental Laboratory Bench Sheet Page 1 

Sample No.: 

Date Collected: 

Location: 

Substrate: 

Taxon 

PHYLUM PORIFERA 

No. 

Notes 

PHYLUM PLATYHELMINTHES 

PHYLUM NEMERTEA 

PHYLUM ANNELIDA 

Class Hirudinea 

Class Oligochaeta 

Class Polychaeta 

PHYLUM SIPUNCULA 

PHYLUM MOLLUSCA 

Class Gastropoda 

Class Bivalvia

Terra Environmental Laboratory Bench Sheet Page 2 

Sample No.: 

Date Collected: 

Taxon 

PHYLUM ARTHROPODA 

Class Insecta 

Order Diptera 

No. 

Notes 

Order Trichoptera 

Order Plecoptera 

Order Coleoptera 

Order Odonata 

Class Crustacea 

Order Tanaidacea 

Order Cumacea 

Order Isopoda 

Order Amphipoda 

Order Decapoda 

Subclass Cirripedia 

Subclass Copepoda 

PHYLUM BRACHIOPODA 

Glottidia pyramidata 

PHYLUM BRYZOA 

PHYLUM ECHINODERMATA 

PHYLUM CHORDATA 

Subphylum Cephalochordata 

Brachiostoma floridae 

OTHER

Appendix 6-B 

Benthic Macroinvertebrates Collected by HBMP 

Updated for data through 2005 

Note: All HBMP benthic identifications are based on a twelve digit identification based on 

the criteria shown below. 

HBMP-ID# 

Phylum# 

Class# 

Order# 

Family# 

Genus# 

Species# 

020101010101 02 01 01 01 01 01

HBMP Benthic Species Identification Master List - 2005 

HBMP-ID# Scientific Name Description Common Name 

000000000000 No organisms present . . 

000000000001 no sample . . 

010201000101 Athenaria sp. Cnidaria . 

010201000102 Athenaria sp. a Cnidaria . 

010202000000 Thenaria sp. Thenaria . 

010202000000 Thenaria sp. . . 

020101010101 Stylochus cf. ellipticus Platyhelminthes Oyster Flatworm 

020101010101 Stylochus cf. ellipticus Platyhelminthes Oyster Flatworm 

020101020101 Euplana gracilis Platyhelminthes . 

020102000000 Tricladida Platyhelminthes . 

030000000001 Nemertea sp. Nemertea Ribbon Worm 

030101010101 Tubulanus palucidus Nemertea Ribbon Worm 

040000000000 Megalomma heterops Annelid . 

040101010101 Leitoscoloplos sp. Annelid . 

040101010102 Leitoscoloplos robustus Annelid . 

040101010103 Leitoscoloplos fragilis Annelid . 

040101010201 Scoloplos rubra Annelid Red Threadworm 

040101020100 Aricidea sp. Annelid . 

040101020101 Aricidea taylori Annelid . 

040101020102 Aricidea philbinae Annelid Unicorn Worm 

040102010101 Ctenodrilus cirrratus Annelid . 

040105020001 Spionidae sp. Annelid . 

040105020101 Carazziella hobsonae Annelid . 

040105020200 Prionospio sp. Annelid . 

040105020201 Prionospio perkinsi Annelid . 

040105020202 Prionospio heterobranchia Annelid . 

040105020301 Scolelepis texana Annelid Palp Worm 

040105020401 Streblospio benedicti Annelid . 

040105020501 Paraprionospio pinnata Annelid . 

040105020600 Polydora ligni Annelid . 

040105020601 Polydora socialis Annelid . 

040105020701 Apoprionospio pygmaea Annelid . 

040105020801 Scolecolepis viridis Annelid . 

040105020901 Spio pettiboneae Annelid . 

040105021001 Spiophanes bombyx Annelid Bee spionid 

040105030101 Magelona pettiboneae Annelid Shovel Headed Worm 

040105070101 Spiochaetopterus c. oculatus Annelid . 

040105080000 Cirratulidae sp. Annelid . 

040105080101 Monticellina dorsobranchialis Annelid . 

040105080200 Caulleriella sp. Annelid . 

040105080300 Cirriformia sp. Annelid . 

040105080301 . . . 

040106010101 Capitella capitata Annelid . 

040106010102 Capitella jonesi Annelid .



040106010201 Heteromastus filiformis Annelid Capitellid Thread Worm 

040106010300 Mediomastus sp. Annelid . 

040106010301 Mediomastus californiensis Annelid . 

040106010302 Mediomastus ambiseta Annelid . 

040106010400 Notomastus sp. Annelid Maitre D' Dorm 

040106010401 Notomastus latericeus Annelid Maitre D' Dorm 

040106010402 Nototmastus hemipodus Annelid . 

040106010403 Notomastus sp. A Annelid . 

040106010501 cf. Decamastus sp. A Annelid 

040106020101 Arenicola cristata Annelid Lugworm 

040106030101 Asychis elongatus Annelid . 

040106030201 Clymenella torquata Annelid . 

040107010101 Armandia maculata Annelid Lancelet Worm 

040108010001 Phyllodocidae sp. Annelid . 

040108010101 Eteone heteropoda Annelid Eteone Worm 

040108010201 Genetyllis castanea Annelid . 

040108010301 Phyllodoce arenae Annelid . 

040108010401 Anaitides longipes Annelid . 

040108010402 Anaitides mucosa Annelid . 

040108010501 Paranaitis polynoides Annelid . 

040108060101 Harmothoe sp.C Annelid . 

040108060102 Malmgreniella sp. B Annelid . 

040108060201 Malmgreniella sp.A Annelid . 

040108070101 Polyodontes lupinus Annelid . 

040108090101 Grubeulepis mexicana Annelid . 

040108100101 Sthenelais sp. A Annelid . 

040108110101 Bhwania heteroseta Annelid . 

040108140001 Hesionidae sp. Annelid . 

040108140101 Podarkeopsis levifuscina Annelid . 

040108140201 Parahesione luteola Annelid . 

040108140301 Podarke obscura Annelid Swift-Footed Worm 

040108140401 Gyptis vittata Annelid . 

040108150101 Sigambra bassi Annelid . 

040108150102 Sigambra tentaculata Annelid . 

040108150201 Synelmis sp. b Annelid . 

040108150301 Ancystrosyllis jonesi Annelid . 

040108160101 Typosyllis cf. amica Annelid . 

040108160102 Typosyllis armillaris Annelid . 

040108160201 Exogone dispar Annelid . 

040108180000 Nereidae sp. Annelid . 

040108180101 Laeonereis culveri Annelid Culver's Sandworm 

040108180201 Neanthes micromma Annelid . 

040108180202 Neanthes succinea Annelid Pile worm 

040108180301 Steninonereis martini Annelid . 

040108180401 Nereis falsa Annelid . 

040108200100 Glycera sp. Annelid .



040108200101 Glycera americana Annelid Blood Worm 

040108210101 Glycinde solitaria Annelid . 

040108240101 Nephtys picta Annelid . 

040108240102 Nephtys simoni Annelid . 

040108240201 Aglaophamus verrilli Annelid Dwarf Sandworm 

040109010101 Pseudeurythoe ambigua Annelid . 

040109010201 Paramphinome sp.b Annelid . 

040111010000 Onuphidae sp. Annelid . 

040111010101 Diopatra cuprea Annelid Plumed Worm 

040111010201 Mooreonuphis cf. nebulosa Annelid . 

040111010301 Kinbergonuphis cf. simoni Annelid . 

040111020101 Lysidice sp. a Annelid . 

040111020201 Marphysa sp. b Annelid . 

040111020202 Marphysa sanguinea Annelid Rockworm 

040111020203 Marphysa cf. belli Annelid . 

040111020301 Nematonereis hebes Annelid . 

040111030101 Lumbrineris sp. d Annelid . 

040111030102 Lumbrineris verrilli Annelid . 

040111070101 Schistomeringos cf. rudolphi Annelid . 

040114010101 Piromis roberti Annelid Bristlecage Worm 

040116020101 Pectinaria gouldii Annelid Ice Cream Cone Worm 

040116030001 Ampharetidae sp. Annelid . 

040116030101 Hobsonia florida Annelid . 

040116030201 Melinna maculata Annelid Spaghetti Mouth Worm 

040116030301 Isolda pulchella Annelid . 

040116040100 Pista sp. Annelid . 

040116040101 Streblosoma hartmanae Annelid . 

040116040301 Polycirrus sp. Annelid . 

040117010000 Sabellidae sp. Annelid . 

040117010101 Branchiomma sp. Annelid . 

040117010200 Potamilla sp. Annelid . 

040117010301 Megalomma pigmentum Annelid . 

040117010302 Megalomma heterops Annelid . 

040117010401 Chone cf. americana Annelid . 

040117020101 cf. Hydroides dianthus Annelid . 

040117020101 Hydroides dianthus Annelid . 

040201010100 Eclipidrilus sp. Oligochaete . 

040201020101 Thalassodrilides gurwitschi Oligochaete . 

040201020201 Tubificidae sp. w/out hairs Oligochaete . 

040201020202 Tubificidae with hairs Oligochaete . 

040201020301 Monopylephorus rubroniveus Oligochaete . 

040201020302 Monopylephorus cf parvus Oligochaete . 

040201020302 Monopylephorus parvus Oligochaete . 

040201020401 Tubificoides brownae Oligochaete . 

040201020402 Tubificodes motei Oligochaete . 

040201020403 Tubificodes ? Oligochaete .



040201020501 Ilyodrilus templetoni Oligochaete . 

040201020601 Limnodrilus hoffmeisteri Oligochaete Tubificid Worm 

040201020701 Aulodrilus pluriseta Oligochaete . 

040201020702 Aulodrilus pigueti Oligochaete . 

040201020801 Limnodriloides barnardi Oligochaete . 

040201020802 Limnodriloides rubicundis Oligochaete . 

040201020901 Tectidrilus squalidus Oligochaete . 

040201021201 Branchiura sowerbyi Oligochaete . 

040201030101 Pristina proboscidea Oligochaete . 

040201030201 Nais communis Oligochaete . 

040201030202 Nais variabilis Oligochaete . 

040201030301 Dero digitata Oligochaete . 

040201030401 Paranais litoralis Oligochaete . 

040202010101 Eclipidrilus palustris Oligochaete . 

040202010201 Lumbriculus variegatus Oligochaete . 

040301010101 Myzobdella uraguayensis Annelid Fish Leech 

040302010101 Glossiphoniidae sp.a Annelid Fish Leech 

040302010201 Helobdella elongata Annelid . 

040302010202 Helobdella stagnalis Annelid . 

040302010203 Helobdella triserialis Annelid . 

050100000000 Gastropoda sp. Gastropod . 

050103030101 Cyclostremiscus pentagonus Gastropod . 

050103030102 Cyclostremiscus suppressus Gastropod . 

050103030201 Vitrinella helicoidea Gastropod . 

050103030202 Vitrinella floridana Gastropod Florida Vitrinella 

050103040101 Cyclostromella humilis Gastropod . 

050103060101 Assiminea succinea Gastropod Atlantic Assiminea 

050104010101 Neritina usnea Gastropod Olive Nerite 

050108010100 Crepidula sp. Gastropod Slippersnail 

050108010101 Crepidula maculosa Gastropod Spotted Slippersnail 

050108010102 Crepidula fornicata Gastropod Common Atlantic Slippersnail 

050108010103 Crepidula plana Gastropod Flat slipper snail 

050109010000 Ancyllidae sp. Gastropod . 

050109010101 Hebetancylis excentricus Gastropod . 

050109010201 Laevapex fuscus Gastropod . 

050109020101 Helisoma anceps Gastropod . 

050110010101 Bittium cf. varium Gastropod Grass cerith 

050111010101 Tectonatica pusilla Gastropod . 

050111010201 Polynices duplicatus Gastropod Moon Snail 

050114010101 Epitonium angulatum Gastropod Angled wentletrap 

050115010101 Microeulimia hemphilli Gastropod . 

050115010201 ? Gastropod . 

050116010701 Eupleura sulcidentata Gastropod Sharp-Rib Drill 

050116011101 Stramonita haemostoma Gastropod . 

050116020101 Pisania tincta Gastropod . 

050116020201 Cantharus cancellarius Gastropod Cancellate Lesser Whelk



050116030101 Astyris lunatus Gastropod . 

050116030201 Parvanchis obesa Gastropod Common Eastern Nassa 

050116040101 Nassarius vibex Gastropod Mottled Dog Whelk 

050116040102 Nassarius albus Gastropod Variable Nassa 

050116050101 Melangena corona Gastropod . 

050116080101 Oliva sayana Gastropod Lettered Olive 

050116080201 Olivella floralia Gastropod . 

050116080202 Olivella ? Gastropod . 

050116080301 Jaspidella blanesi Gastropod . 

050116090101 Prunum apicinum Gastropod Common Atlantic Marginella 

050116090201 Gibberula lavalleena Gastropod Common Atlantic Marginella 

050120010101 Boonea impressa Gastropod Impressed Odostome 

050120010201 Turbonilla cf. punicea Gastropod . 

050120010202 Turbonilla incisa Gastropod Etched Turbonille 

050120010203 Turbonilla cf. viridimaris Gastropod . 

050120010301 Odostomia laevigata Gastropod . 

050120010302 Odostomia cf. gibbosa Gastropod . 

050120010401 Sayella laevigata Gastropod . 

050120010402 Sayella fusca Gastropod . 

050121010101 Rictaxis punctostriatus Gastropod Pitted Baby-Bubble 

050121010201 Bullina cf. torrei Gastropod . 

050121020101 Bulla striata Gastropod Atlantic Bubble 

050121030101 Haminoea succinea Gastropod Amber Glassy-Bubble Snail 

050121030102 Haminoea elegans Gastropod Elegant Glassy-Bubble Snail 

050121030201 Atys riiseana Gastropod . 

050121040101 Acteocina canaliculata Gastropod Channeled Barrel-Bubble 

050122020101 Cyerce cf. cristallina Gastropod . 

050123010101 Hydrobiidae sp. Gastropod Hydrobiid 

050123010201 Pyrgophorus platyrachis Gastropod . 

050123020101 Melanoides tuberculata Gastropod . 

050124000001 Nudibranchia sp. Gastropod . 

050125010101 Menetus dilatatus Gastropod Trumpet Ram's-horn 

050200000001 Bivalvia sp. Bivalve Bivalve 

050201010101 Nucula proxima Bivalve Atlantic Nutclam 

050202010101 Anadara transversa Bivalve Transverse Ark 

050204010101 Amygdalum papyrium Bivalve Atlantic Paper Mussel 

050204010201 Ischadium recurvum Bivalve Hooked Mussel 

050204010301 Geukensia granosissma Bivalve . 

050204010401 Perna viridis Bivalve Green Mussel 

050204020201 Mytilopsis leucophaeta Bivalve Dark False Mussel 

050208010101 Crassostrea virginica Bivalve Eastern Oyster 

050211010101 Parvilucina multilineata Bivalve Many-lined Lucine 

050212010101 Anomia simplex Bivalve Common Jingle Shell 

050215010101 Laevacardium mortoni Bivalve Morton Eggcockle 

050216010101 Mulinia lateralis Bivalve Dwarf Surfclam 

050216010201 Mactra fragilis Bivalve Fragile Surfclam



050218010001 Tellinidae sp. Bivalve Tellin Clam 

050218010101 Macoma constricta Bivalve Constricted Macoma Clam 

050218010102 Macoma tenta Bivalve Elongate Macoma Clam 

050218010201 Tellina cf. alternata Bivalve Alternate Tellin 

050218010202 Tellina cf. versicolor Bivalve Many-Colored Tellin 

050218010203 Tellina tampaensis Bivalve Tampa Tellin 

050218010204 Tellina mera Bivalve Pure Tellin 

050218010301 Ensis minor Bivalve Dwarf razor clam 

050218010401 Donax variabilis Bivalve . 

050218020101 Tagelus divisus Bivalve Purplish Tagelus 

050218020102 Tagelus plebius Bivalve Stout Razor Clam 

050218040101 Semele proficua Bivalve Atlantic Semele 

050218040201 Abra aequalis Bivalve . 

050219010101 Polymesoda carolinae Bivalve Carolina Marsh Clam 

050219010201 Corbicula fluminea Bivalve Asian Clam 

050219020100 Sphaerium sp. Bivalve Clam 

050219020101 Byssanodonta cubensis Bivalve mottled fingernailclam 

050219020200 Pisidium sp. Bivalve . 

050220010101 Parastarte triquetra Bivalve Brown Gemclam 

050220010201 Dosinia elegans Bivalve Elegant Dosinia 

050220010202 Dosinia discus Bivalve Disk Dosinia 

050220010301 Anomalocardia auberiana Bivalve Pointed-Venus 

050220010401 Mercenaria campechiensis Bivalve Southern Qahog 

050220010501 Cyclinella tenuis Bivalve Thin Cyclinella 

050220010601 Chione cancellata Bivalve Cross-barred venus 

050220010602 Chione cf. paphia Bivalve . 

050220020101 Petricola pholadiformis Bivalve False angel wing 

050220030101 Cyrenoidea floridana Bivalve Florida Marsh Clam 

050220040101 Mysella planulata Bivalve Plate Mysella 

050221010101 Corbula contracta Bivalve Contracted Corbula 

050221010102 Corbula swiftiana Bivalve Corbula 

050221010103 Corbula chittyana Bivalve Corbula 

050221020101 Sphenia antillensis Bivalve . 

050221030101 Hiatella cf. azaria Bivalve . 

050221040101 Pholas campechiensis Bivalve . 

050222010101 Cyrtopleura costata Bivalve Angelwing Clam 

050223010101 Lyonsia floridana Bivalve Florida Lyonsia 

050223020101 Bushia elegans Bivalve . 

050223020201 Asthenothaerus hemphilli Bivalve Hemphill Thracid 

060101010101 Hargeria repax Arthropod . 

060101020101 Halmyrapseudes bahamensis Arthropod . 

060102000000 Cumacea sp. Arthropod . 

060102010101 Oxyurostylus cf. smithi Arthropod . 

060102010102 Oxyurostylus lecroyae Arthropod . 

060102030101 Cyclaspis cf. varians Arthropod . 

060102040101 Almyracuma proximoculae Arthropod .



060103010101 Cyathura polita Isopod Sowbug 

060103010201 Xenanthura brevitelson Isopod Sowbug 

060103010301 Amakusanthura magnifica Isopod Sowbug 

060103020101 Exosphaeroma diminuta Isopod Sowbug 

060103020201 Harrieta faxoni Isopod Sowbug 

060103020301 Cassidinidea ovalis Isopod Sowbug 

060103020401 Sphaeroma quadridentatum Isopod Sowbug 

060103020402 Sphaeroma walkeri Isopod . 

060103030100 Asellus sp. Isopod Sowbug 

060103040101 Uromunna hayesi Isopod Sowbug 

060103040102 Uromunna reynoldsi Isopod Sowbug 

060103050101 Edotea triloba Isopod Sowbug 

060103060101 Erichisonella attenuata Isopod Sowbug 

060103070100 Cirolana parva Isopod . 

060104010101 Gammarus mucronatus Amphipod Keeled Scud 

060104010102 Gammarus cf. tigrinis Amphipod Scud 

060104020101 Melita elongata Amphipod Scud 

060104020102 Melita nitida complex Amphipod Scud 

060104020103 cf. melita dentata Amphipod Scud 

060104020201 Elasmopus levis Amphipod Scud 

060104030101 Listriella barnardi Amphipod Scud 

060104030201 Microdeutopus sp. a Amphipod Scud 

060104040101 Batea catharinensis Amphipod Scud 

060104070101 Monoculodes nyei Amphipod Scud 

060104070102 Monoculodes cf. edwardsi Amphipod Scud 

060104070102 Monoculodes edwardsi Amphipod Scud 

060104100101 Eobrolgus spinosus Amphipod Scud 

060104100201 Repoxinius epistomus Amphipod Scud 

060104120101 Ampelisca cf. abdita Amphipod Scud 

060104120102 Ampelisca cf. holmesi Amphipod Scud 

060104120103 Ampelisca vadorum Amphipod Scud 

060104120104 Ampelisca sp. d Amphipod Scud 

060104130101 Lysianopsis alba Amphipod Scud 

060104140101 Acuminodeutopus naglei Amphipod Scud 

060104140201 Grandidierella bonnieroides Amphipod Scud 

060104140301 Lembos smithi Amphipod Scud 

060104150101 Cymadusa compta Amphipod Scud 

060104160101 Gitanopsis cf.lagunae Amphipod Scud 

060104170001 Corophiidae sp. Amphipod Scud 

060104170100 Corophium sp. Amphipod Scud 

060104170101 Corophium louisianum Amphipod Scud 

060104170102 Corophium lacustre Amphipod Scud 

060104170103 Corophium ellisi Amphipod Scud 

060104170201 Erichthonius brasiliensis Amphipod Scud 

060104170202 Erichthonius rubicornis Amphipod Scud 

060104170300 Cerapus sp. Amphipod Scud



060104170301 Cerapus cf. tubularis Amphipod Scud 

060104170401 Monocorophium acherusicum Amphipod Scud 

060104180101 Hyale sp. a Amphipod Scud 

060104190101 Microprotopus raneyi Amphipod Scud 

060104200000 Caprellidae sp. Amphipod Scud 

060104200101 Caprella cf. penantis Amphipod Scud 

060104200201 Paracaprella tenuis Amphipod Scud 

060104210101 Orchestia cf. grillus Amphipod Scud 

060104210101 Eudevenopus honduranus Amphipod Scud 

060104230101 Hyalella azteca Amphipod . 

060105010000 Penaeidae sp. Decopod Shrimp 

060105010101 Penaeus duorarum Decopod American Pink Shrimp 

060105010102 Penaeus cf. aztecus Decopod American Pink Shrimp 

060105020100 Procambarus sp. Decopod . 

060105030000 Palaemonidae Decopod Prawn 

060105030100 Palaemonetes sp. Decopod Prawn 

060105030101 Palaemonetes paludosus Decopod Prawn 

060105030200 Periclimenes sp. Decopod Prawn 

060105040000 Alpheidae sp. Decopod Snapping Shrimp 

060105040101 Alpheus cf. estuariensis Decopod Snapping Shrimp 

060105040201 Leptalpheus forceps Decopod Snapping Shrimp 

060105050101 Hippolyte zostericola Decopod . 

060105060101 Ambidexter symmetricus Decopod . 

060105070101 Ogyrides alphaerostris Decopod . 

060105090101 Euceramus praelongus Decopod Porcelain Crab 

060105090201 Petrolisthes cf. armatus Decopod . 

060105100000 Paguridae sp. Decopod Hermit Crab 

060105100101 Pagurus piercei Decopod Hermit Crab 

060105100102 Pagurus ? Decopod Hermit Crab 

060105110100 Clibanarius sp. Decopod 

060105120101 Persephona mediterranea Decopod Purse Crab 

060105130000 Xanthidae sp. Decopod Crab 

060105130101 Rhithropanopeus harrisii Decopod Estuarine Mud Crab 

060105130201 Panopeus herbstii Decopod Common Mud Crab 

060105130301 Eurytium limosum Decopod White-Clawed Mud Crab 

060105130401 Eurypanopeus depressus Decopod Flatback Mud Crab 

060105140101 Pinnixa sp. Decopod Pea crab 

060105140102 Pinnixa sayana Decopod Pea crab 

060105140103 Pinnixa chaetopterana Decopod Pea crab 

060105140201 Pinnotheres ostreum Decopod Pea crab 

060105150100 Uca sp. Decopod Fiddler Crab 

060105160101 Callinectes sapidus Decopod Blue crab 

060106010000 Mysidacea sp. Mysid Opossum Shrimp 

060106010100 Bowmaniella sp. Mysid Opossum Shrimp 

060106010201 Mysidopsis bahia Mysid Opossum Shrimp 

060106010202 Mysidopsis bigelowi Mysid Opossum Shrimp



060106010203 Mysidopsis almyra Mysid . 

060106010301 Taphromysis bowmani Mysid Opossum Shrimp 

060107010101 Balanus improvisus Arthropod Bay Barnacle 

060107010102 Balanus eburneus Arthropod Ivory barnacle 

060107010103 Balanus amphitrite Arthropod Barnacle 

060202010000 Entomobryidae sp. Insect . 

060203010100 Brachycercus sp. Insect Mayfly 

060203010200 Caenis sp. Insect Mayfly 

060203010300 Tricorythodes sp. Insect Mayfly 

060203020000 Baetidae sp. Insect Mayfly 

060203030000 Heptageniidae sp. Insect Mayfly 

060204000000 Odonata sp. Insect Dragonfly/Damselfly 

060204020000 Gomphidae sp. Insect Clubtail 

060204020100 Gomphus sp. Insect Clubtail 

060204020201 Aphylla williamsoni Insect . 

060204030100 Macromia sp. Insect . 

060204040100 Epitheca sp. Insect . 

060204050100 Coenagrionidae sp. Insect . 

060204050201 Argia moesta Insect . 

060204060000 Libellulidae sp. Insect . 

060210010100 Polycentropus sp. Insect Trumpetnet Caddisfly 

060210010201 Cyrnellus fraternus Insect Trumpetnet Caddisfly 

060210020000 Leptoceridae sp. Insect Caddisfly 

060210020100 Oecetis sp. Insect 

Long Horned Casemaker 

Caddisfly 

060210020200 Nectopsyche sp. Insect Caddisfly 

060210030100 Cheumatopsyche sp. Insect Net-Spinning Caddisfly 

060210040000 Hyproptilidae sp. Insect Caddisfly 

060210040100 Orthotrichia Sp. Insect . 

060211010000 Pyralidae sp. Insect . 

060213010100 Dubiraphia sp. Insect . 

060213010200 Stenelmis sp. Insect Riffle Bettle 

060213020101 Dinuetis sp. Insect Riffle Bettle 

060214010101 Chaoborus punctipennis Insect Phantom Midge 

060214020000 Chironomidae sp. Insect Midge 

060214020100 Chironomus sp. Insect . 

060214020200 Cladotanytarsus sp. Insect . 

060214020300 Tanytarsus sp. Insect . 

060214020400 Cryptochironomus sp. Insect . 

060214020500 Polypedilum sp. Insect . 

060214020501 Polypedilum halterale Insect . 

060214020502 Polypedilum scalaenum Insect . 

060214020503 Polypedilum tritum Insect . 

060214020504 Polypedilum convictum Insect . 

060214020600 Procladius sp. Insect . 

060214020700 Coelotanypus sp. Insect .



060214020701 Coelotanypus tricolor Insect . 

060214020800 Djalmabatista sp. Insect . 

060214020900 Rheotanytarsus sp. Insect . 

060214021000 Fissimentum sp. Insect . 

060214021100 Glyptotendipes sp Insect . 

060214021201 Ablabesmyia cinctipes Insect . 

060214021202 Ablabesmyia mallochi Insect . 

060214021203 Ablabesmyia ramphe Insect . 

060214021301 Tanypus clavatus Insect . 

060214021302 Tanypus neopunctipennis Insect . 

060214021400 Tribelos sp. Insect . 

060214021401 Tribelos fuscicorne Insect . 

060214021402 Tribelos jucundum Insect . 

060214021500 Dicrotendipes sp. Insect . 

060214021600 Pagastiella sp. Insect . 

060214021700 Kiefferulus sp. Insect . 

060214021800 Cryptotendipes sp. Insect . 

060214021900 Paralauterborniella sp. Insect . 

060214022000 Goeldichironomus sp. Insect . 

060214022100 Pentaneura sp. Insect . 

060214022200 Stempellina sp. Insect . 

060214022300 Stenochironomus sp. Insect . 

060214022400 Cricotopus sp. Insect . 

060214022500 Clinotanypus sp. Insect . 

060214022600 Nilothauma sp. Insect . 

060214022700 Paracladopelma sp. Insect . 

060214022800 Corynoneura sp. Insect . 

060214022901 Asheum beckae Insect . 

060214023001 Axarus sp. Insect . 

060214023100 Psuedochironomus sp. Insect . 

060214023200 Larsia sp. Insect . 

060214030100 Palpomyia/Bezzia sp. Insect . 

060214030200 Cuculoides sp. . . 

060214040000 Dolichopodidae sp. Insect . 

060301010101 Limulus polyphemus . Atlantic Horseshoe Crab 

070203010101 Mellita isometra Echinoderm . 

070301010001 Amphiuridae sp. Echinoderm . 

070301010101 Amphioplus squamata Echinoderm Brittle Star 

070301010102 Amphioplus thromboides Echinoderm Brittle Star 

070301010103 Amphioplus sepultus Echinoderm Brittle Star 

070301010201 Ophiophragmus filograneus Echinoderm Brittle Star 

070301010301 Micropholus atra Echinoderm . 

070301010400 Amphilochus sp. Echinoderm . 

070400000001 Holothuroidea sp. Echinoderm Sea Cucumber 

070401010101 Thyone cf. crassidisca Echinoderm Sea Cucumber 

070402010101 Synaptula hydriformis Echinoderm Sea Cucumber



070402010201 Leptosynapta tenuis Echinoderm Sea Cucumber 

090000000000 Phoronida Phoronids Horseshoe Worm 

090101010101 Phoronis archectecta Phoronida Horseshoe Worm 

100100000000 Ascidiacea sp. Chordata . 

100100000001 Enteropneusta sp. Chordata . 

100201010101 cf. mogula occidentalis Chordata Sandy Sea Squirt 

140101010101 Glottidea pyramidata Brachiopod Lamp Shell 

150000000000 Sipunculida sp. Sipunculida . 

-99999-1 Lab Error #1

Master EPCHC Benthic Species Identification List 

Nodccode Name Phylum Class Order Infraord Family 

360000000000 1 PORIFERA 

360000009500 Porifera Sp. E Porifera 

360000009600 Porifera Sp. D Porifera 

360000009700 Porifera Sp. C Porifera 

360000009800 Porifera Sp. B Porifera 

360000009900 Porifera Sp. A Porifera 

370000000001 Cnidaria Sp. B 

370100000000 2 HYDROZOA 

374000000000 Anthozoa 

375800000000 Actiniaria 

375800000010 Actiniaria Sp. A 

375800000020 Actiniaria Sp. B 

375900000000 Athenaria 

376000000000 Thenaria 

376000000001 Thenaria A 

376000000002 Thenaria B 

376000000003 Thenaria C 

376000000004 Thenaria D 

390100000000 Turbellaria Platyhelminthes Turbellaria 

390100000095 Turbellaria F Platyhelminthes Turbellaria 

390100000096 Turbellaria E Platyhelminthes Turbellaria 

390100000097 Turbellaria C Platyhelminthes Turbellaria 

390100000098 Turbellaria B Platyhelminthes Turbellaria 

390100000099 Turbellaria A Platyhelminthes Turbellaria 

390603010000 Stylochus Sp. 

430000000000 Nemertea Sp. 

430000007800 Nemertea Y 

430000007900 Nemertea X 

430000008000 Nemertea W



430000008100 Prostoma Sp. 

430000008200 Nemertea V 

430000008300 Nemertea U 

430000008400 Nemertea T 

430000008500 Nemertea S 

430000008600 Nemertea R 

430000008700 Nemertea O 

430000008800 Nemertea Q 

430000008900 Nemertea N 

430000009000 Nemertea P 

430000009100 Nemertea L 

430000009200 Nemertea M 

430000009300 Nemertea G 

430000009400 Nemertea F 

430000009500 Nemertea E 

430000009600 Nemertea I 

430000009700 Nemertea K 

430000009800 Nemertea B 

430000009900 Nemertea A 

430000010000 Nemertea J 

500000000000 ANNELIDA 

500100000000 3 POLYCHAETA Annelida Polychaeta 

500100000001 Polychaeta Sp. 1 Annelida Polychaeta 

500102000000 4 POLYNOIDAE Annelida Polychaeta Polynoidae 

500102000001 Polynoidae A Annelida Polychaeta Polynoidae 

500102000002 Polynoidae B Annelida Polychaeta Polynoidae 

500102080000 Harmothoe Sp. Annelida Polychaeta Polynoidae 

500102081000 Harmothoe Lunulata Annelida Polychaeta Polynoidae 

500102081200 Harmothoe Aculeata Annelida Polychaeta Polynoidae 

500102110400 Lepidonotus Sublevis Annelida Polychaeta Polynoidae 

500102110500 Lepidonotus Variabilis Annelida Polychaeta Polynoidae



500102190100 Lepidasthenia Commensalis Annelida Polychaeta Polynoidae 

500102260200 Malmgreniella Maccraryae Annelida Polychaeta Polynoidae 

500102260300 Malmgreniella Taylori Annelida Polychaeta Polynoidae 

500102410100 Phyllohartmania Taylori Annelida Polychaeta Polynoidae 

500102960000 Polynoidae Genus D Annelida Polychaeta Polynoidae 

500103000000 5 POLYODONTIDAE Annelida Polychaeta Polyodontidae 

500103020000 Polyodontes Sp. Annelida Polychaeta Polyodontidae 

500103020100 Polyodontes Lupinus Annelida Polychaeta Polyodontidae 

500105000000 6 EULEPETHIDAE Annelida Polychaeta 

500105010200 Grubeulepis Mexicana Annelida Polychaeta 

500106000000 7 SIGALIONIDAE Annelida Polychaeta Sigalionidae 

500106009000 Sigalion Sp. Annelida Polychaeta Sigalionidae 

500106009900 Sigalion A Annelida Polychaeta Sigalionidae 

500106030200 Sthenelais Boa Annelida Polychaeta Sigalionidae 

500106039000 Sthenelais Sp. Annelida Polychaeta Sigalionidae 

500106039900 Sthenelais A Annelida Polychaeta Sigalionidae 

500106069800 Thalenessa B Annelida Polychaeta Sigalionidae 

500106069900 Sigalion B Annelida Polychaeta Sigalionidae 

500108030200 Bhawania Heteroseta Annelida Polychaeta Chrysopetalidae 

500110000000 8 AMPHINOMIDAE Annelida Polychaeta Amphinomidae 

500110030200 Pseudeurythoe Ambigua Annelida Polychaeta Amphinomidae 

500110049800 Paramphinome B Annelida Polychaeta Amphinomidae 

500111010800 Euphrosine Triloba Annelida Polychaeta 

500113000000 9 PHYLLODOCIDAE Annelida Polychaeta Phyllodocidae 

500113010200 Phyllodoce Groenlandica Annelida Polychaeta Phyllodocidae 

500113011200 Phyllodoce Longipes Annelida Polychaeta Phyllodocidae 

500113020700 Eteone Heteropoda Annelida Polychaeta Phyllodocidae 

500113020800 Eteone Lactea Annelida Polychaeta Phyllodocidae 

500113021100 Eteone Foliasa Annelida Polychaeta Phyllodocidae 

500113070100 Nereiphylla Castanea Annelida Polychaeta Phyllodocidae



500113080100 Paranaitis Speciosa Annelida Polychaeta Phyllodocidae 

500113080300 Paranaitis Gardineri Annelida Polychaeta Phyllodocidae 

500113100100 Nereiphylla Fragilis Annelida Polychaeta Phyllodocidae 

500113100400 Nereiphylla A Annelida Polychaeta Phyllodocidae 

500113110000 Eumida A Annelida Polychaeta Phyllodocidae 

5001131101cf Eumida Cf. Sanguinea Annelida Polychaeta Phyllodocidae 

500113141000 Phyllodoce Arenae Annelida Polychaeta Phyllodocidae 

500121000000 10 HESIONIDAE Annelida Polychaeta Hesionidae 

500121010000 Gyptis Sp. Annelida Polychaeta Hesionidae 

500121010800 Gyptis Crypta Annelida Polychaeta Hesionidae 

500121011000 Gyptis Plurisetis Annelida Polychaeta Hesionidae 

500121070100 Parahesione Luteola Annelida Polychaeta Hesionidae 

500121150200 Ophiodromus Obscura Annelida Polychaeta Hesionidae 

500121160000 Hesione Sp. Annelida Polychaeta Hesionidae 

500121160100 Hesione Picta Annelida Polychaeta Hesionidae 

500121190200 Podarkeopsis Levifuscina Annelida Polychaeta Hesionidae 

500122010003 Ancistrosyllis C Annelida Polychaeta Pilargiidae 

500122010200 Ancistrosyllis Hartmanae Annelida Polychaeta Pilargiidae 

500122010300 Ancistrosyllis Jonesi Annelida Polychaeta Pilargiidae 

500122010600 Ancistrosyllis Carolinensis Annelida Polychaeta Pilargiidae 

500122020000 Sigambra Sp. Annelida Polychaeta Pilargiidae 

500122020100 Sigambra Tentaculata Annelida Polychaeta Pilargiidae 

500122020400 Sigambra Bassi Annelida Polychaeta Pilargiidae 

500122030100 Pilargis Berkeleyae Annelida Polychaeta Pilargiidae 

500122030200 Pilargis Pacifica Annelida Polychaeta Pilargiidae 

500122040100 Cabira Incerta Annelida Polychaeta Pilargiidae 

500122059800 Synelmis Ewingi Annelida Polychaeta Pilargiidae 

500122090200 Litocorsa Antennata Annelida Polychaeta Pilargiidae 

500122099900 Litocorsa A Annelida Polychaeta Pilargiidae 

500123000000 11 SYLLIDAE Annelida Polychaeta Syllidae 

500123000001 Autolytinae Annelida Polychaeta Syllidae



500123010000 Autolytus Sp. Annelida Polychaeta Syllidae 

500123010001 Procerae A Annelida Polychaeta Syllidae 

500123010100 Procerae Cornuta Annelida Polychaeta Syllidae 

500123020000 Pionosyllis Sp. Annelida Polychaeta Syllidae 

500123020700 Pionosyllis Gesae Annelida Polychaeta Syllidae 

500123021200 Pionosyllis D Annelida Polychaeta Syllidae 

500123021300 Pionosyllis B Annelida Polychaeta Syllidae 

500123021500 Pionosyllis Nr. Ehlersiaeformis Annelida Polychaeta Syllidae 

500123030001 Syllis A Annelida Polychaeta Syllidae 

500123030002 Syllis B Annelida Polychaeta Syllidae 

500123030003 Haplosyllis Sp. Annelida Polychaeta Syllidae 

500123030200 Syllis Gracilis Annelida Polychaeta Syllidae 

500123030300 Haplosyllis Spongicola Annelida Polychaeta Syllidae 

500123030600 Syllis Cornuta Annelida Polychaeta Syllidae 

500123031300 Syllis Beneliahuae Annelida Polychaeta Syllidae 

500123040000 Trypanosyllis Sp. Annelida Polychaeta Syllidae 

500123040500 Trypanosyllis Coeliaca Annelida Polychaeta Syllidae 

500123040900 Trypanosyllis Parvidentata Annelida Polychaeta Syllidae 

500123050000 Syllis Sp. Annelida Polychaeta Syllidae 

500123050100 Syllis Alternata Annelida Polychaeta Syllidae 

500123050200 Syllis Armillaris Annelida Polychaeta Syllidae 

500123050201 Syllis Ferrugina Annelida Polychaeta Syllidae 

500123051200 Syllis Prolifera Annelida Polychaeta Syllidae 

5001230515cf Syllis Cf. Lutea Annelida Polychaeta Syllidae 

500123051600 Syllis Aciculata Annelida Polychaeta Syllidae 

500123052300 Syllis Vittata Annelida Polychaeta Syllidae 

500123055000 Syllis Annularis Annelida Polychaeta Syllidae 

500123070000 Exogone Sp. Annelida Polychaeta Syllidae 

500123070001 Exogone A Annelida Polychaeta Syllidae 

500123070100 Exogone Dispar Annelida Polychaeta Syllidae 

500123070300 Exogone Lourei Annelida Polychaeta Syllidae 

500123070900 Exogone Arenosa Annelida Polychaeta Syllidae



500123071000 Exogone Atlantica Annelida Polychaeta Syllidae 

500123071100 Exogone Breviantennata Annelida Polychaeta Syllidae 

500123072000 Exogone C Annelida Polychaeta Syllidae 

500123080000 Sphaerosyllis Sp. Annelida Polychaeta Syllidae 

500123080700 Sphaerosyllis Aciculata Annelida Polychaeta Syllidae 

500123081000 Sphaerosyllis Glandulata Annelida Polychaeta Syllidae 

500123081100 Sphaerosyllis Taylori Annelida Polychaeta Syllidae 

500123081600 Sphaerosyllis Bilobata Annelida Polychaeta Syllidae 

500123081700 Sphaerosyllis Longicauda Annelida Polychaeta Syllidae 

5001230817nr Sphaerosyllis Nr. Longicauda Annelida Polychaeta Syllidae 

500123081800 Sphaerosyllis Piriferopsis Annelida Polychaeta Syllidae 

500123081900 Sphaerosyllis Riseri? Annelida Polychaeta Syllidae 

500123082000 Sphaerosyllis Labyrinthophila Annelida Polychaeta Syllidae 

500123089800 Sphaerosyllis B Annelida Polychaeta Syllidae 

500123089900 Sphaerosyllis A Annelida Polychaeta Syllidae 

500123090000 Brania Sp. Annelida Polychaeta Syllidae 

500123090001 Grubeosyllis Sp. Annelida Polychaeta Syllidae 

500123090200 Grubeosyllis Clavata Annelida Polychaeta Syllidae 

500123090300 Brania Wellfleetensis Annelida Polychaeta Syllidae 

500123090800 Grubeosyllis Swedmarki Annelida Polychaeta Syllidae 

500123099400 Grubeosyllis A Annelida Polychaeta Syllidae 

500123099500 Grubeosyllis Nitidula Annelida Polychaeta Syllidae 

500123099600 Grubeosyllis Mediodentata Annelida Polychaeta Syllidae 

500123099700 Grubeosyllis Rugulosa Annelida Polychaeta Syllidae 

500123099800 Grubeosyllis Vietezi Annelida Polychaeta Syllidae 

500123099900 Brania A Annelida Polychaeta Syllidae 

500123130000 Odontosyllis Sp. Annelida Polychaeta Syllidae 

500123130700 Odontosyllis Enopla Annelida Polychaeta Syllidae 

500123150700 Syllides Fulvus? Annelida Polychaeta Syllidae 

500123151000 Syllides Floridanus Annelida Polychaeta Syllidae 

500123160500 Streptosyllis Pettiboneae Annelida Polychaeta Syllidae 

500123170000 Parapionosyllis Sp. Annelida Polychaeta Syllidae



500123170100 Parapionosyllis Longicirrata Annelida Polychaeta Syllidae 

500123179500 Parapionosyllis Uelebackerae Annelida Polychaeta Syllidae 

500123179800 Parapionosyllis Floridana Annelida Polychaeta Syllidae 

500123250301 Opistosyllis A Annelida Polychaeta Syllidae 

500123260200 Plakosyllis Quadrioculata Annelida Polychaeta Syllidae 

500123270100 Branchiosyllis Oculata Annelida Polychaeta Syllidae 

500123270200 Branchiosyllis Exilis Annelida Polychaeta Syllidae 

500123310100 Dentatisyllis Carolinae Annelida Polychaeta Syllidae 

500123320000 Myrianida Annelida Polychaeta Syllidae 

500124000000 12 NEREIDIDAE Annelida Polychaeta Nereididae 

500124000001 Nereis Sp. Annelida Polychaeta Nereididae 

500124010000 Ceratonereis Sp. Annelida Polychaeta Nereididae 

500124010300 Ceratonereis Irritabilis Annelida Polychaeta Nereididae 

500124011200 Ceratonereis (Composetia?) A Annelida Polychaeta Nereididae 

500124030500 Nereis Arenaceodentata Annelida Polychaeta Nereididae 

500124030600 Nereis (Neanthes) Sp. Annelida Polychaeta Nereididae 

500124030700 Nereis Acuminata Annelida Polychaeta Nereididae 

500124030900 Nereis Succinea Annelida Polychaeta Nereididae 

500124040301 Nereis Pelagica Occidentalis Annelida Polychaeta Nereididae 

500124041400 Nereis Falsa Annelida Polychaeta Nereididae 

500124041500 Neanthes Micromma Annelida Polychaeta Nereididae 

500124041600 Nereis Lamellosa Annelida Polychaeta Nereididae 

500124041800 Nereis Riisei Annelida Polychaeta Nereididae 

500124050300 Platynereis Dumerilii Annelida Polychaeta Nereididae 

500124060001 Ceratocephale A Annelida Polychaeta Nereididae 

500124080100 Laeonereis Culveri Annelida Polychaeta Nereididae 

500124100100 Websterinereis Tridentata Annelida Polychaeta Nereididae 

500124120100 Stenoninereis Martini Annelida Polychaeta Nereididae 

500124130000 Nicon Sp. Annelida Polychaeta Nereididae 

500124130100 Nicon Lackeyi Annelida Polychaeta Nereididae 

500124140500 Perinereis Floridana Annelida Polychaeta Nereididae



500124180100 Rullierinereis Mexicana Annelida Polychaeta Nereididae 

500124250100 Namalycastis Abiuma Annelida Polychaeta Nereididae 

500124980000 Nereididae 1A Annelida Polychaeta Nereididae 

500125000000 NEPHTYIDAE Annelida Polychaeta Nephtyidae 

5001250112cf Nephtys Cf. Hombergii Annelida Polychaeta Nephtyidae 

500125011400 Nephtys Bucera Annelida Polychaeta Nephtyidae 

500125011600 Nephtys Magellanica Annelida Polychaeta Nephtyidae 

500125011700 Nephtys Picta Annelida Polychaeta Nephtyidae 

500125011800 Nephtys Squamosa Annelida Polychaeta Nephtyidae 

500125012300 Nephtys Incisa Annelida Polychaeta Nephtyidae 

500125012500 Nephtys Simoni Annelida Polychaeta Nephtyidae 

500125012600 Nephtys Cryptomma Annelida Polychaeta Nephtyidae 

500125030300 Aglaophamus Verrilli Annelida Polychaeta Nephtyidae 

500127000000 13 GLYCERIDAE Annelida Polychaeta Glyceridae 

500127010100 Glycera Capitata Annelida Polychaeta Glyceridae 

500127010400 Glycera Americana Annelida Polychaeta Glyceridae 

500127010500 Glycera Dibranchiata Annelida Polychaeta Glyceridae 

500127019500 Glycera E Annelida Polychaeta Glyceridae 

500127019600 Glycera B Annelida Polychaeta Glyceridae 

500127020100 Hemipodus Roseus Annelida Polychaeta Glyceridae 

500128000000 14 GONIADIDAE Annelida Polychaeta Goniadidae 

500128010400 Glycinde Solitaria Annelida Polychaeta Goniadidae 

500128020500 Goniada Littorea Annelida Polychaeta Goniadidae 

500128030000 Goniadella Sp. Annelida Polychaeta Goniadidae 

500128049800 Ophioglycera B Annelida Polychaeta Goniadidae 

500128050100 Goniadides Carolinae Annelida Polychaeta Goniadidae 

500128050200 Goniadides A Annelida Polychaeta Goniadidae 

500129000000 15 ONUPHIDAE Annelida Polychaeta Onuphidae 

500129010000 Onuphis Sp. Annelida Polychaeta Onuphidae 

500129010001 Mooreonuphis Sp. Annelida Polychaeta Onuphidae 

500129010701 Onuphis Ememita Oculata Annelida Polychaeta Onuphidae



500129010900 Mooreonuphis Pallidula Annelida Polychaeta Onuphidae 

500129012000 Onuphis A Annelida Polychaeta Onuphidae 

500129020100 Diopatra Cuprea Annelida Polychaeta Onuphidae 

500129070100 Americonuphis Magna Annelida Polychaeta Onuphidae 

5001291401cf Mooreonuphis Cf. Nebulosa Annelida Polychaeta Onuphidae 

500129140200 Mooreonuphis A Annelida Polychaeta Onuphidae 

500129150000 Kinbergonuphis Sp. Annelida Polychaeta Onuphidae 

500129150100 Kinbergonuphis Simoni Annelida Polychaeta Onuphidae 

500129159800 Kinbergonuphis A Annelida Polychaeta Onuphidae 

500129159900 Kinbergonuphis C Annelida Polychaeta Onuphidae 

500130011900 Eunice Rubra Annelida Polychaeta Eunicidae 

500130020100 Marphysa Sanguinea? Annelida Polychaeta Eunicidae 

500130020202 Marphysa Nr. Belli Annelida Polychaeta Eunicidae 

500130030000 Lysidice Sp. Annelida Polychaeta Eunicidae 

500130030100 Lysidice Ninetta Annelida Polychaeta Eunicidae 

500130050200 Nematonereis Hebes Annelida Polychaeta Eunicidae 

500131010000 Lumbrineris Sp. Annelida Polychaeta Lumbrineridae 

500131010400 Lumbrineris Latreilli Annelida Polychaeta Lumbrineridae 

500131011300 Lumbrineris Tenuis Annelida Polychaeta Lumbrineridae 

500131011500 Lumbrineris Impatiens Annelida Polychaeta Lumbrineridae 

500131012300 Lumbrineris Ernesti Annelida Polychaeta Lumbrineridae 

500131012400 Lumbrineris Verrilli Annelida Polychaeta Lumbrineridae 

500131012500 Lumbrineris Coccinea Annelida Polychaeta Lumbrineridae 

500131012600 Lumbrineris Januarii Annelida Polychaeta Lumbrineridae 

500131019000 Lumbrineris E Annelida Polychaeta Lumbrineridae 

500131019600 Lumbrineris D Annelida Polychaeta Lumbrineridae 

500131019800 Lumbrineris B Annelida Polychaeta Lumbrineridae 

500131019900 Lumbrineris A Annelida Polychaeta Lumbrineridae 

500133010300 Drilonereis Longa Annelida Polychaeta Arabellidae 

500133010500 Drilonereis Magna Annelida Polychaeta Arabellidae 

500133010815 Drilonereis Cylindrica Annelida Polychaeta Arabellidae 

500133010900 Drilonereis E Annelida Polychaeta Arabellidae



500133020000 Arabella Sp. Annelida Polychaeta Arabellidae 

500133020001 Arabella Multidentata Annelida Polychaeta Arabellidae 

500133020100 Arabella Iricolor Annelida Polychaeta Arabellidae 

500133020200 Arabella Mutans Annelida Polychaeta Arabellidae 

500134000000 16 OENONIDAE Annelida Polychaeta 

500134010100 Lysarete Brasiliensis Annelida Polychaeta 

500136000001 Dorvilleidae A Annelida Polychaeta Dorvilleidae 

500136010000 Dorvillea Sp. Annelida Polychaeta Dorvilleidae 

500136020500 Protodorvillea Bifida Annelida Polychaeta Dorvilleidae 

500136040000 Ophryotrocha Sp. Annelida Polychaeta Dorvilleidae 

500136040100 Ophryotrocha Puerilis Annelida Polychaeta Dorvilleidae 

500136049900 Ophryotrocha A Annelida Polychaeta Dorvilleidae 

500136050000 Schistomeringos Sp. Annelida Polychaeta Dorvilleidae 

500136050300 Schistomeringos Pectinata Annelida Polychaeta Dorvilleidae 

5001360504cf Schistomeringos Rudolphi Annelida Polychaeta Dorvilleidae 

500136070000 Pettiboneia Sp. Annelida Polychaeta Dorvilleidae 

500136070200 Pettiboneia Duofurca Annelida Polychaeta Dorvilleidae 

500140000000 17 ORBINIIDAE Annelida Polychaeta Orbiniidae 

500140010400 Leitoscoloplos Robustus Annelida Polychaeta Orbiniidae 

500140020000 Naineris Sp. Annelida Polychaeta Orbiniidae 

500140020800 Naineris Setosa Annelida Polychaeta Orbiniidae 

500140030000 Scoloplos Sp. Annelida Polychaeta Orbiniidae 

500140030700 Scoloplos Rubra Annelida Polychaeta Orbiniidae 

500140030900 Scoloplos Texana Annelida Polychaeta Orbiniidae 

500140039600 Scoloplos D Annelida Polychaeta Orbiniidae 

500140039700 Scoloplos C Annelida Polychaeta Orbiniidae 

500140050400 Orbinia Riseri Annelida Polychaeta Orbiniidae 

500140050500 Orbinia Americana Annelida Polychaeta Orbiniidae 

500140160000 Leitoscoloplos Sp. Annelida Polychaeta Orbiniidae 

500140160300 Leitoscoloplos Fragilis Annelida Polychaeta Orbiniidae 

500141000000 18 PARAONIDAE Annelida Polychaeta Paraonidae



500141020000 Aricidea Sp. Annelida Polychaeta Paraonidae 

500141020010 Aricidea (Allia) A Annelida Polychaeta Paraonidae 

500141020100 Aricidea Suecica Annelida Polychaeta Paraonidae 

5001410206cf Aricidea Cf. Catherinae Annelida Polychaeta Paraonidae 

500141021400 Aricidea Fragilis Annelida Polychaeta Paraonidae 

500141021500 Aricidea Lopezi Annelida Polychaeta Paraonidae 

500141021700 Aricidea Quadrilobata Annelida Polychaeta Paraonidae 

500141022100 Aricidea Philbinae Annelida Polychaeta Paraonidae 

500141022200 Aricidea Taylori Annelida Polychaeta Paraonidae 

500141022700 Aricidea Allisdari Annelida Polychaeta Paraonidae 

500141030200 Paraonis Fulgens Annelida Polychaeta Paraonidae 

500141050200 Paraonella Sp. Annelida Polychaeta Paraonidae 

500141060000 Cirrophorus Sp. Annelida Polychaeta Paraonidae 

500141060300 Cirrophorus Lyra? Annelida Polychaeta Paraonidae 

500141060500 Cirrophorus Branchiatus? Annelida Polychaeta Paraonidae 

500141060600 Cirrophorus Furcatus? Annelida Polychaeta Paraonidae 

500141130100 Aricidea Cerrutii Annelida Polychaeta Paraonidae 

500143000000 19 SPIONIDAE Annelida Polychaeta Spionidae 

500143020100 Laonice Cirrata Annelida Polychaeta Spionidae 

500143040000 Polydora Sp. Annelida Polychaeta Spionidae 

500143040200 Dipolydora Socialis Annelida Polychaeta Spionidae 

500143041100 Polydora Cornuta Annelida Polychaeta Spionidae 

500143041200 Polydora Websteri Annelida Polychaeta Spionidae 

500143050000 Prionospio Sp. Annelida Polychaeta Spionidae 

500143050200 Prionospio Multibranchiata Annelida Polychaeta Spionidae 

500143050300 Prionospio Heterobranchia Annelida Polychaeta Spionidae 

500143050600 Prionospio Steenstrupi Annelida Polychaeta Spionidae 

500143050700 Apoprionospio Pygmaea Annelida Polychaeta Spionidae 

500143051000 Prionospio Cristata Annelida Polychaeta Spionidae 

500143051100 Apoprionospio Dayi Annelida Polychaeta Spionidae 

500143051700 Prionospio Perkinsi Annelida Polychaeta Spionidae



500143070000 Spio Sp. Annelida Polychaeta Spionidae 

500143070400 Spio Setosa Annelida Polychaeta Spionidae 

500143070600 Spio Pettiboneae Annelida Polychaeta Spionidae 

500143070700 Spio Limnicola Annelida Polychaeta Spionidae 

500143070900 Spio Armata Annelida Polychaeta Spionidae 

500143100100 Spiophanes Bombyx Annelida Polychaeta Spionidae 

500143100500 Spiophanes Wigleyi? Annelida Polychaeta Spionidae 

500143100600 Spiophanes Missionensis Annelida Polychaeta Spionidae 

500143150000 Pseudopolydora Sp. Annelida Polychaeta Spionidae 

500143150001 Pseudopolydora A Annelida Polychaeta Spionidae 

500143150002 Pseudopolydora B Annelida Polychaeta Spionidae 

500143159900 Pseudopolydora Antennata? Annelida Polychaeta Spionidae 

500143170100 Paraprionospio Pinnata Annelida Polychaeta Spionidae 

500143170200 Paraprionospio A Annelida Polychaeta Spionidae 

500143180100 Streblospio Benedicti Annelida Polychaeta Spionidae 

500143190100 Dispio Uncinata Annelida Polychaeta Spionidae 

500143200000 Scolelepis Sp. Annelida Polychaeta Spionidae 

500143200600 Scolelepis Texana Annelida Polychaeta Spionidae 

500143220200 Aonides Mayaguezensis Annelida Polychaeta Spionidae 

500143230100 Microspio Pigmentata Annelida Polychaeta Spionidae 

500143260000 Minuspio (Prionospio) Sp. Annelida Polychaeta Spionidae 

500143270600 Carazziella Hobsonae Annelida Polychaeta Spionidae 

500144000000 MAGELONIDAE Annelida Polychaeta Magelonidae 

500144010000 Magelona Sp. Annelida Polychaeta Magelonidae 

500144010600 Magelona Pettiboneae Annelida Polychaeta Magelonidae 

500144010700 Magelona Riojai Annelida Polychaeta Magelonidae 

500144019000 Magelona B Annelida Polychaeta Magelonidae 

500144019100 Magelona A Annelida Polychaeta Magelonidae 

500144019200 Magelona H Annelida Polychaeta Magelonidae 

500144019300 Magelona I Annelida Polychaeta Magelonidae 

500144019400 Magelona C Annelida Polychaeta Magelonidae 

500144019500 Magelona G Annelida Polychaeta Magelonidae



500146010000 Poecilochaetus Sp. Annelida Polychaeta Poecilochaetidae 

500146010100 Poecilochaetus Johnsoni Annelida Polychaeta Poecilochaetidae 

500149010100 Chaetopterus Pergamentaceus Annelida Polychaeta Chaetopteridae 

500149030200 Spiochaetopterus Costarum Annelida Polychaeta Chaetopteridae 

500149040000 Mesochaetopterus Sp. Annelida Polychaeta Chaetopteridae 

500149040300 Mesochaetopterus Capensis Annelida Polychaeta Chaetopteridae 

500150000000 20 CIRRATULIDAE Annelida Polychaeta Cirratulidae 

500150010000 Cirratulus Sp. Annelida Polychaeta Cirratulidae 

500150010400 Cirriformia Grandis Annelida Polychaeta Cirratulidae 

500150020000 Caulleriella Sp. Annelida Polychaeta Cirratulidae 

500150020200 Caulleriella Cf. Alata Annelida Polychaeta Cirratulidae 

500150020600 Caulleriella Zetlandica Annelida Polychaeta Cirratulidae 

500150029400 Caulleriella C Annelida Polychaeta Cirratulidae 

500150029500 Caulleriella E Annelida Polychaeta Cirratulidae 

500150029600 Caulleriella D Annelida Polychaeta Cirratulidae 

500150029700 Caulleriella A Annelida Polychaeta Cirratulidae 

500150029800 Caulleriella 1 Annelida Polychaeta Cirratulidae 

500150029900 Caulleriella B Annelida Polychaeta Cirratulidae 

500150030000 Tharyx Sp. Annelida Polychaeta Cirratulidae 

500150030100 Aphelochaeta Sp? Annelida Polychaeta Cirratulidae 

500150030600 Tharyx Annulosus Annelida Polychaeta Cirratulidae 

500150031000 Monticellina Dorsobranchialis Annelida Polychaeta Cirratulidae 

500150039900 Tharyx E Annelida Polychaeta Cirratulidae 

500150040000 Chaetozone Sp. Annelida Polychaeta Cirratulidae 

500150049600 Chaetozone D Annelida Polychaeta Cirratulidae 

500150049800 Chaetozone B Annelida Polychaeta Cirratulidae 

500150049900 Chaetozone A Annelida Polychaeta Cirratulidae 

500150060000 Cirriformia Sp. Annelida Polychaeta Cirratulidae 

500150069400 Cirriformia C Annelida Polychaeta Cirratulidae 

500150069500 Cirriformia Sp. 1 Annelida Polychaeta Cirratulidae 

500150069600 Cirriformia D Annelida Polychaeta Cirratulidae



500150069800 Cirriformia B Annelida Polychaeta Cirratulidae 

500150069900 Cirriformia A Annelida Polychaeta Cirratulidae 

500151000000 21 ACROCIRRIDAE Annelida Polychaeta 

5001510202cf Macrochaeta Cf. Clavicornis Annelida Polychaeta 

500154050400 Piromis Roberti Annelida Polychaeta Flabelligeridae 

500158000000 22 OPHELIIDAE Annelida Polychaeta Opheliidae 

500158010100 Ophelina Cf. Acuminata Annelida Polychaeta Opheliidae 

500158020000 Armandia Sp. Annelida Polychaeta Opheliidae 

500158020300 Armandia Agilis Annelida Polychaeta Opheliidae 

500158020400 Armandia Maculata Annelida Polychaeta Opheliidae 

500158040600 Travisia Hobsonae Annelida Polychaeta Opheliidae 

500158080000 Polyopthalmus Sp. Annelida Polychaeta Opheliidae 

500160000000 23 CAPITELLIDAE Annelida Polychaeta Capitellidae 

500160010100 Capitella Capitata Annelida Polychaeta Capitellidae 

500160010101 Capitella Jonesi (=Sp. A) Annelida Polychaeta Capitellidae 

500160020100 Heteromastus Filiformis Annelida Polychaeta Capitellidae 

500160030000 Notomastus Sp. Annelida Polychaeta Capitellidae 

5001600302cf Notomastus Cf. Tenuis Annelida Polychaeta Capitellidae 

500160030300 Notomastus Lineatus Annelida Polychaeta Capitellidae 

500160030600 Notomastus Latericeus Annelida Polychaeta Capitellidae 

500160030700 Notomastus Hemipodus Annelida Polychaeta Capitellidae 

500160031000 Notomastus Americanus Annelida Polychaeta Capitellidae 

500160031100 Notomastus Daueri Annelida Polychaeta Capitellidae 

500160032000 Notomastus Lobatus Annelida Polychaeta Capitellidae 

500160039700 Notomastus A Annelida Polychaeta Capitellidae 

500160039800 Notomastus B Annelida Polychaeta Capitellidae 

500160040000 Mediomastus Sp. Annelida Polychaeta Capitellidae 

500160040100 Mediomastus Ambiseta Annelida Polychaeta Capitellidae 

500160040200 Mediomastus Californiensis Annelida Polychaeta Capitellidae 

500160070100 Capitella Jonesi Annelida Polychaeta Capitellidae 

500160160000 Scyphoproctus Sp. Annelida Polychaeta Capitellidae



500160160001 Scyphoproctus A Annelida Polychaeta Capitellidae 

500160240000 Capitomastus Sp. Annelida Polychaeta Capitellidae 

500160980000 Capitellidae Genus A2 Annelida Polychaeta Capitellidae 

500160990000 Capitellidae Genus A Annelida Polychaeta Capitellidae 

500160991000 Capitellidae Genus A1 Annelida Polychaeta Capitellidae 

500162020100 Arenicola Cristata Annelida Polychaeta 

500163000000 24 MALDANIDAE Annelida Polychaeta Maldanidae 

500163010000 Asychis Sp. Annelida Polychaeta Maldanidae 

500163010300 Sabaco Americanus Annelida Polychaeta Maldanidae 

500163010600 Asychis Elongatus Annelida Polychaeta Maldanidae 

500163010800 Asychis Lunulata Annelida Polychaeta Maldanidae 

500163020200 Clymenella Torquata Annelida Polychaeta Maldanidae 

500163070000 Petaloproctus Sp. Annelida Polychaeta Maldanidae 

500163080300 Axiothella Mucosa Annelida Polychaeta Maldanidae 

500163080900 Axiothella A Annelida Polychaeta Maldanidae 

500163100001 Rhodine A Annelida Polychaeta Maldanidae 

500163110700 Dasybranchus Lumbricoides Annelida Polychaeta Maldanidae 

500163111000 Euclymene Sp. A Annelida Polychaeta Maldanidae 

500163180000 Lumbriclymenae (Praxillura?) Annelida Polychaeta Maldanidae 

500163220100 Branchioasychis Americana Annelida Polychaeta Maldanidae 

500164000000 25 OWENIIDAE Annelida Polychaeta Oweniidae 

500164019900 Owenia Fusiformis Annelida Polychaeta Oweniidae 

500164040200 Galathowenia Oculata Annelida Polychaeta Oweniidae 

500165000000 SABELLARIIDAE Annelida Polychaeta Sabellariidae 

500165020000 Sabellaria Sp. Annelida Polychaeta Sabellariidae 

500165029800 Sabellaria C Annelida Polychaeta Sabellariidae 

500165029900 Sabellaria A Annelida Polychaeta Sabellariidae 

500166030200 Pectinaria Gouldii Annelida Polychaeta Pectinariidae 

500167000000 26 AMPHARETIDAE Annelida Polychaeta Ampharetidae 

500167000001 Ampharetidae A Annelida Polychaeta Ampharetidae 

500167030300 Amphicteis Gunneri Annelida Polychaeta Ampharetidae



500167030900 Hobsonia Florida Annelida Polychaeta Ampharetidae 

500167050000 Melinna Sp. Annelida Polychaeta Ampharetidae 

500167050100 Melinna Cristata Annelida Polychaeta Ampharetidae 

500167050400 Melinna Maculata Annelida Polychaeta Ampharetidae 

500167210100 Isolda Pulchella Annelida Polychaeta Ampharetidae 

500168000000 27 TEREBELLIDAE Annelida Polychaeta Terebellidae 

500168000001 Terebellidae A Annelida Polychaeta Terebellidae 

500168020500 Eupolymnia Nebulosa Annelida Polychaeta Terebellidae 

500168070100 Pista Cristata Annelida Polychaeta Terebellidae 

500168070200 Pista Fasciata Annelida Polychaeta Terebellidae 

500168071100 Pista Quadrilobata Annelida Polychaeta Terebellidae 

500168080000 Polycirrus Sp. Annelida Polychaeta Terebellidae 

500168080500 Polycirrus Hematodes Annelida Polychaeta Terebellidae 

500168080900 Polycirrus Carolinensis Annelida Polychaeta Terebellidae 

500168081200 Polycirrus Plumosa Annelida Polychaeta Terebellidae 

500168089500 Polycirrus E Annelida Polychaeta Terebellidae 

500168089600 Polycirrus D Annelida Polychaeta Terebellidae 

500168089700 Polycirrus C Annelida Polychaeta Terebellidae 

500168089800 Polycirrus B Annelida Polychaeta Terebellidae 

500168089900 Polycirrus A Annelida Polychaeta Terebellidae 

500168130000 Lanassa Sp. Annelida Polychaeta Terebellidae 

500168160000 Lysilla Sp. Annelida Polychaeta Terebellidae 

500168160anr Lysilla Nr. Sp. A Annelida Polychaeta Terebellidae 

500168170000 Proclea Sp. Annelida Polychaeta Terebellidae 

500168200000 Loimia Sp. Annelida Polychaeta Terebellidae 

500168200100 Loimia Medusa Annelida Polychaeta Terebellidae 

500168200200 Loimia Viridis Annelida Polychaeta Terebellidae 

500168220300 Terebella Rubra Annelida Polychaeta Terebellidae 

500168250400 Streblosoma Hartmanae Annelida Polychaeta Terebellidae 

500168250500 Streblosoma Verrilli Annelida Polychaeta Terebellidae 

500168990000 Polycirrinae Annelida Polychaeta Terebellidae



500169010100 Terebellides Stroemi Annelida Polychaeta 

500169020100 Trichobranchus Glacialis Annelida Polychaeta 

500170000000 28 SABELLIDAE Annelida Polychaeta Sabellidae 

500170010000 Chone Sp. Annelida Polychaeta Sabellidae 

5001700107cf Chone Cf. Americana Annelida Polychaeta Sabellidae 

500170019900 Chone A Annelida Polychaeta Sabellidae 

500170040000 Megalomma Sp. Annelida Polychaeta Sabellidae 

500170040100 Branchiomma Sp. Annelida Polychaeta Sabellidae 

500170040200 Megalomma Bioculatum Annelida Polychaeta Sabellidae 

500170040600 Megalomma Pigmentum Annelida Polychaeta Sabellidae 

500170049900 Megalomma A Annelida Polychaeta Sabellidae 

500170060000 Potamilla Sp. Annelida Polychaeta Sabellidae 

500170060001 Potamilla A Annelida Polychaeta Sabellidae 

500170070000 Pseudopomatilla Sp. Annelida Polychaeta Sabellidae 

500170070300 Potamilla Cf. Reniformis Annelida Polychaeta Sabellidae 

500170080002 Sabella B Annelida Polychaeta Sabellidae 

500170080500 Sabella Melanostigma Annelida Polychaeta Sabellidae 

500170130001 Fabricia A Annelida Polychaeta Sabellidae 

500170190700 Fabricinuda Trilobata Annelida Polychaeta Sabellidae 

500170270000 Dialychone Sp? Annelida Polychaeta Sabellidae 

500170280000 Demonax Sp. Annelida Polychaeta Sabellidae 

500170280100 Demonax Micropthalma Annelida Polychaeta Sabellidae 

500170300000 Pseudobranchiomma Sp. Annelida Polychaeta Sabellidae 

500170990000 Fabriciinae Annelida Polychaeta Sabellidae 

500173000000 29 SERPULIDAE Annelida Polychaeta Serpulidae 

500173040000 Serpula Sp. Annelida Polychaeta Serpulidae 

500173060200 Spirorbis Spirillum Annelida Polychaeta Serpulidae 

500173060400 Neodexiospira Steueri Annelida Polychaeta Serpulidae 

500173090100 Hydroides Dianthus Annelida Polychaeta Serpulidae 

500173150000 Pomatoceros Sp. Annelida Polychaeta Serpulidae 

500173150100 Pomatoceros Americanus Annelida Polychaeta Serpulidae



500174029900 Questa Sp. A Annelida Polychaeta Questidae 

500174990000 Questidae Genus A Annelida Polychaeta Questidae 

500176010000 Boguea Sp. Annelida Polychaeta Bogueidae 

500176010100 Boguea Enigmatica Annelida Polychaeta Bogueidae 

500178000000 SPIRORBIDAE Annelida Polychaeta 

500200009800 Polygordius Sp. (Archiannelida 

500200009900 Archiannelida Sp. A 

500300000000 30 OLIGOCHAETA 

500300000002 Oligochaeta B 

500300000003 Oligochaeta C 

500300000004 Oligochaeta A 

500901000000 31 ENCHYTRAEIDAE Annelida Oligochaeta Enchytraeidae 

500901030000 Grania Sp. Annelida Oligochaeta Enchytraeidae 

500902000000 32 TUBIFICIDAE Annelida Oligochaeta Tubificidae 

500902009800 Tubificidae W/O Cap. Setae Annelida Oligochaeta Tubificidae 

500902020800 Tubificoides Motei Annelida Oligochaeta Tubificidae 

500902049800 Tubificoides B Annelida Oligochaeta Tubificidae 

500902049900 Tubificoides A Annelida Oligochaeta Tubificidae 

500902070000 Limnodriloides Sp. Annelida Oligochaeta Tubificidae 

500902070200 Limnodriloides Vespertinus Annelida Oligochaeta Tubificidae 

500902070300 Limnodriloides Rubicundus Annelida Oligochaeta Tubificidae 

500902070400 Limnodriloides Barnardi Annelida Oligochaeta Tubificidae 

500902070500 Limnodriloides Baculatus Annelida Oligochaeta Tubificidae 

500902070600 Limnodriloides Monothecus Compl Annelida Oligochaeta Tubificidae 

500902070700 Limnodriloides Uniampulatus Annelida Oligochaeta Tubificidae 

500902079900 Limnodriloides Anxius Annelida Oligochaeta Tubificidae 

500902090100 Tubificoides Brownae Annelida Oligochaeta Tubificidae 

500902090400 Tubificoides Wasselli Annelida Oligochaeta Tubificidae 

500902140100 Thalassodrilides Eneri Annelida Oligochaeta Tubificidae 

500902140200 Thalassodrilides Gurwitschi Annelida Oligochaeta Tubificidae 

500902150100 Tectidrilus Bari Annelida Oligochaeta Tubificidae



500902160000 Heterodrilus Sp. Annelida Oligochaeta Tubificidae 

500902160100 Heterodrilus Bulbiporus Annelida Oligochaeta Tubificidae 

500902160200 Heterodrilus Occidentalis Annelida Oligochaeta Tubificidae 

500902169700 Heterodrilus Pentcheffi Annelida Oligochaeta Tubificidae 

500902169800 Heterodrilus B Annelida Oligochaeta Tubificidae 

500902169900 Heterodrilus A Annelida Oligochaeta Tubificidae 

500902179800 Bathydrilus Adriaticus Annelida Oligochaeta Tubificidae 

500902179900 Bathydrilus A Annelida Oligochaeta Tubificidae 

500902180100 Parakaketio Longipustatus Annelida Oligochaeta Tubificidae 

500902209600 Inanidrilus Leukodermatus Annelida Oligochaeta Tubificidae 

500902209700 Inanidrilus Bulbosus Annelida Oligochaeta Tubificidae 

500902209800 Inanidrilus Sp. B Annelida Oligochaeta Tubificidae 

500902209900 Inanidrilus Sp. A Annelida Oligochaeta Tubificidae 

500902230100 Pectinodrilus Molestus Annelida Oligochaeta Tubificidae 

500902260000 Smithsonidrilus Sp. Annelida Oligochaeta Tubificidae 

500902260100 Smithsonidrilus Marina Complex Annelida Oligochaeta Tubificidae 

500902269900 Smithsonidrilus Sp. A Annelida Oligochaeta Tubificidae 

500902280100 Tectidrilus Squalidus Annelida Oligochaeta Tubificidae 

500902310000 Olavius Sp. Annelida Oligochaeta Tubificidae 

500902310100 Olavius Caudatus Annelida Oligochaeta Tubificidae 

500902490000 Coralliodrilus Sp. A Annelida Oligochaeta Tubificidae 

500902990000 Phallodrilinae Annelida Oligochaeta Tubificidae 

500902999800 Phallodrilinae Sp. B Annelida Oligochaeta Tubificidae 

500902999900 Phallodrilinae Sp. A Annelida Oligochaeta Tubificidae 

500903000000 33 NAIDIDAE Annelida Oligochaeta Naididae 

500903090100 Stephensonia Trivandrana? Annelida Oligochaeta Naididae 

501200000000 34 HIRUDINEA Annelida Hirudinea 

501401070100 Myzobdella Lugubris 

501601020100 Mooreobdella Melanostoma 

501601030100 Erpobdella Puncta 

510000000000 35 GASTROPODA Mollusca Gastropoda



510212000000 TURBINIDAE Mollusca Gastropoda 

510212010100 Didianema Pauli Mollusca Gastropoda 

510306010000 Viviparus Sp. Mollusca Gastropoda 

510306010200 Viviparus Georgianus Mollusca Gastropoda 

510308000000 36 VALVATIDAE Mollusca Gastropoda 

510308010000 Valvata Sp. Mollusca Gastropoda 

510310010000 Littorina Mollusca Gastropoda 

510313000000 37 HYDROBIIDAE Mollusca Gastropoda Hydrobiidae 

510313050100 Littoridinops Palustris Mollusca Gastropoda Hydrobiidae 

510313070000 Lyogyrus Sp. Mollusca Gastropoda Hydrobiidae 

510313210100 Pyrgophorus Platyrachis Mollusca Gastropoda Hydrobiidae 

510317010000 Amnicola Mollusca Gastropoda 

510320050300 Schwartziella Catesbyana Mollusca Gastropoda 

510320060000 Sayella Sp. Mollusca Gastropoda 

510320060300 Sayella Fusca Mollusca Gastropoda 

510320060600 Sayella Hemphilli Mollusca Gastropoda 

510321010300 Assiminea Succinea Mollusca Gastropoda 

510323000000 38 VITRINELLIDAE Mollusca Gastropoda 

510323009900 Vitrinellidae Sp. A Mollusca Gastropoda 

510323020500 Vitrinella Floridana Mollusca Gastropoda 

510323030000 Cyclostremiscus Sp. Mollusca Gastropoda 

510323030100 Cyclostremiscus ?Pentagonus Mollusca Gastropoda 

510323040100 Solariorbis Infracarinata Mollusca Gastropoda 

510323050000 Teinostoma Sp. Mollusca Gastropoda 

510323050700 Teinostoma ?Nesacum Mollusca Gastropoda 

510323090200 Cochliolepis Striata Mollusca Gastropoda 

510323120200 Cyclostremiscus Suppressus Mollusca Gastropoda 

510323130100 Macromphalina Floridanus Mollusca Gastropoda 

510336030000 Caecum Sp. Mollusca Gastropoda Caecidae 

510336030100 Caecum Pulchellum Mollusca Gastropoda Caecidae 

510336030300 Caecum Imbricatum Mollusca Gastropoda Caecidae



510336030400 Caecum Johnsoni Mollusca Gastropoda Caecidae 

510336030700 Caecum Nitidum Mollusca Gastropoda Caecidae 

510336031500 Caecum Strigosum Mollusca Gastropoda Caecidae 

510336031600 Caecum Nr. Insularum Mollusca Gastropoda Caecidae 

510336031700 Caecum Nr. Carolinianum Mollusca Gastropoda Caecidae 

510338010100 Melanoides Tuberculatus Mollusca Gastropoda 

510340010000 Elima Sp. Mollusca Gastropoda 

510343010100 Modulus Modulus Mollusca Gastropoda 

510344020200 Cerithidea Scalariformis Mollusca Gastropoda 

510346000000 Cerithiidae Mollusca Gastropoda Cerithiidae 

510346013000 Bittiolum Varium Mollusca Gastropoda Cerithiidae 

510346060100 Cerithium Atratum Mollusca Gastropoda Cerithiidae 

510346061300 Cerithium Muscarum Mollusca Gastropoda Cerithiidae 

510350010000 Epitonium Sp. Mollusca Gastropoda 

510350010800 Epitonium Rupicolum Mollusca Gastropoda 

510350011000 Epitonium Angulatum Mollusca Gastropoda 

510353000000 39 EULIMIDAE Mollusca Gastropoda Eulimidae 

510353010000 Melanella Sp. Mollusca Gastropoda Eulimidae 

510353010200 Melanella B Mollusca Gastropoda Eulimidae 

510353010500 Melanella ?Intermedia Mollusca Gastropoda Eulimidae 

510353010800 Melanella Cf. Arcuata Mollusca Gastropoda Eulimidae 

510353011000 Melanella Gracilis Mollusca Gastropoda Eulimidae 

510353019900 Melanella A Mollusca Gastropoda Eulimidae 

510353020200 Strombiformis Bilineatus Mollusca Gastropoda Eulimidae 

510353020400 Microeulima Hemphilli Mollusca Gastropoda Eulimidae 

510353020500 Eulima Bifasciatus Mollusca Gastropoda Eulimidae 

510353040100 Niso Aglees Mollusca Gastropoda Eulimidae 

510353060100 Vitreolina Arcuata Mollusca Gastropoda Eulimidae 

510353990100 Oceanida Inglei Mollusca Gastropoda Eulimidae 

510358010100 Strombus Alatus Mollusca Gastropoda 

510364010200 Calyptraea Centralis Mollusca Gastropoda Calyptraeidae



510364020000 Crepidula Sp. Mollusca Gastropoda Calyptraeidae 

510364020400 Crepidula Fornicata Mollusca Gastropoda Calyptraeidae 

510364020700 Crepidula Plana Mollusca Gastropoda Calyptraeidae 

510364020800 Crepidula Aculeata Mollusca Gastropoda Calyptraeidae 

510364021000 Crepidula Maculosa Mollusca Gastropoda Calyptraeidae 

510376020400 Tectonatica Pusilla Mollusca Gastropoda Naticidae 

510376040000 Euspira Sp. Mollusca Gastropoda Naticidae 

510376040700 Neverita Duplicata Mollusca Gastropoda Naticidae 

510376050100 Sinum Perspectivum Mollusca Gastropoda Naticidae 

510501060100 Eupleura Caudata Mollusca Gastropoda 

510501060200 Eupleura Sulcidentata Mollusca Gastropoda 

510501100300 Chicoreus Pomum Mollusca Gastropoda 

510503020700 Astrys Lunulata Mollusca Gastropoda Columbellidae 

510503030000 Anachis Mollusca Gastropoda Columbellidae 

510503030300 Parvanachis Obesa Mollusca Gastropoda Columbellidae 

510503030600 Costoanachis Lafresnayi Mollusca Gastropoda Columbellidae 

510503032800 Costoanachis Semiplicata Mollusca Gastropoda Columbellidae 

510503040500 Aesopus Stearnsii Mollusca Gastropoda Columbellidae 

510504040100 Cantharus Multangulus Mollusca Gastropoda 

510507010801 Busycotypus Spiratus Pyruloides Mollusca Gastropoda 

510507030000 Melongena Sp. Mollusca Gastropoda 

510507030100 Melongena Corona Mollusca Gastropoda 

510508010000 Nassarius Sp. Mollusca Gastropoda 

510508010200 Nassarius Vibex Mollusca Gastropoda 

510508010800 Nassarius Albus Mollusca Gastropoda 

510510010000 Olivella Sp. Mollusca Gastropoda Olividae 

510510010001 Jaspidella Blanesi Mollusca Gastropoda Olividae 

510510010300 Olivella Dealbata (=Pusilla) Mollusca Gastropoda Olividae 

510510010800 Olivella Floralia Mollusca Gastropoda Olividae 

510510010900 Olivella Mutica Mollusca Gastropoda Olividae 

510510011000 Olivella Pusilla Mollusca Gastropoda Olividae 

510510011200 Olivella Nivea Mollusca Gastropoda Olividae



510510020100 Oliva Sayana Mollusca Gastropoda Olividae 

510515010200 Granulina Hadria Mollusca Gastropoda Marginellidae 

510515020300 Dentimargo Aureocinctus Mollusca Gastropoda Marginellidae 

510515020600 Gibberula Lavalleenana Mollusca Gastropoda Marginellidae 

510515021400 Prunum Apicinum Mollusca Gastropoda Marginellidae 

510602000000 TURRIDAE Mollusca Gastropoda 

510602110400 Kurtziella Limonitella Mollusca Gastropoda 

510602110500 Rubellatoma Diomedea Mollusca Gastropoda 

510602140400 Pilsbryspira Leucosyoma Mollusca Gastropoda 

510602180800 Pyrgocythara Plicosa Mollusca Gastropoda 

510602260100 Stellatoma Stellata Mollusca Gastropoda 

510603010601 Conus Stearnsi Mollusca Gastropoda 

510604010100 Terebra Dislocata Mollusca Gastropoda 

510604010200 Terebra Protexta Mollusca Gastropoda 

510622401000 Pyrgospira Tampaensis Mollusca Gastropoda 

510622402000 Pyrgospira Ostrearum Mollusca Gastropoda 

510801010000 Odostomia Spp. Mollusca Gastropoda Pyramidellidae 

510801012700 Odostomia Cf. Bisultralis Mollusca Gastropoda Pyramidellidae 

510801013200 Odostomia Gibbosa Mollusca Gastropoda Pyramidellidae 

510801013500 Boonea Seminuda Mollusca Gastropoda Pyramidellidae 

510801014000 Odostomia Laevigata Mollusca Gastropoda Pyramidellidae 

510801014600 Odostomia Sp. A Mollusca Gastropoda Pyramidellidae 

510801014800 Odostomia Producta Mollusca Gastropoda Pyramidellidae 

510801015000 Eulimastoma Weberi Mollusca Gastropoda Pyramidellidae 

510801015100 Eulimastoma Teres Mollusca Gastropoda Pyramidellidae 

510801015500 Eulimastoma Engonium Mollusca Gastropoda Pyramidellidae 

510801015600 Eulimastoma Ergonia? Mollusca Gastropoda Pyramidellidae 

510801020000 Turbonilla Sp. Mollusca Gastropoda Pyramidellidae 

510801020900 Turbonilla Interrupta Mollusca Gastropoda Pyramidellidae 

510801021300 Turbonilla Conradi Mollusca Gastropoda Pyramidellidae 

510801021400 Turbonilla Cf. Dalli Mollusca Gastropoda Pyramidellidae 

510801021500 Turbonilla Hemphilli Mollusca Gastropoda Pyramidellidae



510801021600 Turbonilla Incisa Mollusca Gastropoda Pyramidellidae 

510801022000 Turbonilla Constricta Mollusca Gastropoda Pyramidellidae 

5108010225nr Turbonilla Nr. Mighelsi Mollusca Gastropoda Pyramidellidae 

510801030300 Pyramidella Crenulata Mollusca Gastropoda Pyramidellidae 

510801050100 Eulimastoma Sp. Mollusca Gastropoda Pyramidellidae 

510801108900 Turbonilla Sp. K Mollusca Gastropoda Pyramidellidae 

510801109100 Turbonilla Sp. I Mollusca Gastropoda Pyramidellidae 

510801112300 Turbonilla Viridaria Mollusca Gastropoda Pyramidellidae 

510801140200 Boonea Impressa Mollusca Gastropoda Pyramidellidae 

511000000001 Lephalapsidea Sp. Mollusca Gastropoda 

511001040300 Rictaxis Punctostriatus Mollusca Gastropoda Acteonidae 

511004000000 40 CYLICHNIDAE Mollusca Gastropoda 

511004000001 Acteocinidae Sp. A Mollusca Gastropoda 

511004010000 Acteocina Sp. Mollusca Gastropoda 

511004010300 Acteocina Canaliculata Mollusca Gastropoda 

511004010500 Acteocina ?Atriata Mollusca Gastropoda 

511004010600 Acteocina Bidentata Mollusca Gastropoda 

511004012400 Tornatina Inconspicua Mollusca Gastropoda 

511004020000 Cylichna Sp. Mollusca Gastropoda 

511011010100 Bulla Striata Mollusca Gastropoda Bullidae 

511012010000 Haminoea Sp. Mollusca Gastropoda 

511012010400 Haminoea Succinea Mollusca Gastropoda 

511012010500 Haminoea Elegans Mollusca Gastropoda 

511012011100 Haminoea Antillarum Mollusca Gastropoda 

511411030100 Hebetoncylus Excentricus Mollusca Gastropoda Ancylidae 

511412010000 Gyraulus Sp. Mollusca Gastropoda Planorbidae 

511412020000 Heliosoma Mollusca Gastropoda Planorbidae 

511413060100 Physa Mollusca Gastropoda Physidae 

512402000001 Aplysiidae Sp. A Mollusca Gastropoda 

512402020100 Aplysia Sp. Mollusca Gastropoda 

512402050000 Anaspidea Sp. Mollusca Gastropoda



513003010200 Doris Verrucosa Mollusca Gastropoda 

513409000001 Polyceratidae Sp. Mollusca Gastropoda 

514107000000 41 TERGIPODIDAE Mollusca Gastropoda 

514107040100 Tenellia Fuscata Mollusca Gastropoda 

518100000000 42 OPISTHOBRANCHIA Mollusca Gastropoda 

530302030100 Ischnochiton Papillosus Mollusca Polyplacophor A 

530401020300 Acanthochitona Pygmaea Mollusca Polyplacophor A 

530501010000 Acanthopleura Granulata Mollusca Polyplacophor A 

540000000000 43 APLACOPHORA 

540105010100 Meiomenia Arenicola 

550000000000 44 BIVALVIA Mollusca Bivalvia 

550000000001 Bivalvia Sp. 1 (M Lat?) Mollusca Bivalvia 

550000000002 Bivalvia Sp 2 (Nucula?) Mollusca Bivalvia 

550000000003 Bivalvia Sp. C Mollusca Bivalvia 

550000000004 Bivalvia Sp. A Mollusca Bivalvia 

550202021400 Nucula Crenulata Mollusca Bivalvia Nuculidae 

550204020400 Nuculana Acuta Mollusca Bivalvia Nuculanidae 

550401010400 Solemya Occidentalis Mollusca Bivalvia Solemyidae 

550601020100 Anadara Transversa Mollusca Bivalvia 

550601050200 Barbatia ?Candida Mollusca Bivalvia 

550701000000 45 MYTILIDAE Mollusca Bivalvia Mytilidae 

550701020500 Crenella Decussata Mollusca Bivalvia Mytilidae 

550701041300 Musculus Lateralis Mollusca Bivalvia Mytilidae 

550701090200 Brachidontes Exustus Mollusca Bivalvia Mytilidae 

550701100100 Amygdalum Papyrium Mollusca Bivalvia Mytilidae 

550702000000 46 PINNIDAE Mollusca Bivalvia 

550702010100 Atrina Seminuda Mollusca Bivalvia 

550902000000 47 ISOGNOMIDAE Mollusca Bivalvia 

550902010100 Isognomon Radiatus Mollusca Bivalvia 

550909020200 Anomia Simplex Mollusca Bivalvia



551002020200 Ostrea Equestris Mollusca Bivalvia 

551202010000 Alasmidonta Mollusca Bivalvia Unionidae 

551501000001 Lucinidae Sp. Mollusca Bivalvia Lucinidae 

551501010200 Parvilucina Multilineata Mollusca Bivalvia Lucinidae 

551501020200 Lucinoma Filosa Mollusca Bivalvia Lucinidae 

551501030500 Lucinisca Nassula Mollusca Bivalvia Lucinidae 

551501030600 Lucinisca Muricata Mollusca Bivalvia Lucinidae 

551501060100 Lucina Amianta Mollusca Bivalvia Lucinidae 

551501080100 Parvilucina Costata Mollusca Bivalvia Lucinidae 

551501080200 Codakia Orbiculata Mollusca Bivalvia Lucinidae 

551502001020 Crassostrea Virginica Mollusca Bivalvia 

551505010300 Diplodonta Semiaspera Mollusca Bivalvia Ungulinidae 

551505010500 Diplodonta Punctata Mollusca Bivalvia Ungulinidae 

551509010100 Neaeromya Sp. Mollusca Bivalvia Lasaeidae 

551509010300 Orobitella Floridana Mollusca Bivalvia Lasaeidae 

551509010900 Orobitella Limpida Mollusca Bivalvia Lasaeidae 

551510011000 Mysella Planulata Mollusca Bivalvia 

551510019900 Erycina Floridana Mollusca Bivalvia 

551517060100 Pteromeris Perplanna Mollusca Bivalvia 

551517070100 Pleuromeris Tridentata Mollusca Bivalvia 

551517080100 Carditamera Floridana Mollusca Bivalvia 

551520010200 Crassinella Lunulata Mollusca Bivalvia 

551522040000 Laevicardium Sp. Mollusca Bivalvia Cardiidae 

551522040100 Laevicardium Mortoni Mollusca Bivalvia Cardiidae 

551522070200 Trachycardium Egmontianum Mollusca Bivalvia Cardiidae 

551525030100 Mulinia Lateralis Mollusca Bivalvia Mactridae 

551525040100 Rangia Cuneata Mollusca Bivalvia Mactridae 

551525050100 Anatina Anatina Mollusca Bivalvia Mactridae 

551525060100 Mactrotoma Fragilis Mollusca Bivalvia Mactridae 

551525070100 Raeta Plicatella Mollusca Bivalvia Mactridae 

551528010100 Ervilia Concentrica Mollusca Bivalvia 

551529020200 Solen Viridis Mollusca Bivalvia



551529030200 Ensis Minor Mollusca Bivalvia 

551531000000 48 TELLINIDAE Mollusca Bivalvia Tellinidae 

551531010000 Macoma Sp. Mollusca Bivalvia Tellinidae 

551531012000 Macoma Tenta Mollusca Bivalvia Tellinidae 

551531012100 Macoma Constricta Mollusca Bivalvia Tellinidae 

551531012500 Macoma Brevifrons Mollusca Bivalvia Tellinidae 

551531020000 Tellina Sp. Mollusca Bivalvia Tellinidae 

551531020700 Tellina Iris Mollusca Bivalvia Tellinidae 

551531020800 Tellina Lineata Mollusca Bivalvia Tellinidae 

551531020900 Tellina Versicolor Mollusca Bivalvia Tellinidae 

551531021000 Tellina Alternata Mollusca Bivalvia Tellinidae 

551531021200 Tellina Texana Mollusca Bivalvia Tellinidae 

551531021400 Tellina Tampaensis Mollusca Bivalvia Tellinidae 

551531021600 Tellina Nitens? Mollusca Bivalvia Tellinidae 

551531021800 Tellina Sybaritica Mollusca Bivalvia Tellinidae 

551531022800 Tellina Tenella Mollusca Bivalvia Tellinidae 

551531023100 Tellina Exerythra? Mollusca Bivalvia Tellinidae 

551531024000 Tellina Similis Mollusca Bivalvia Tellinidae 

551531029900 Tellina Sp. A Mollusca Bivalvia Tellinidae 

551531040100 Tellidora Cristata Mollusca Bivalvia Tellinidae 

551533020000 Tagelus Sp. Mollusca Bivalvia Psammobiidae 

551533020100 Tagelus Plebeius Mollusca Bivalvia Psammobiidae 

551533020200 Tagelus Divisus Mollusca Bivalvia Psammobiidae 

551535020100 Abra Aequalis Mollusca Bivalvia Semelidae 

551535030201 Cumingia Tellinoides Vanhyningi Mollusca Bivalvia Semelidae 

551535060100 Semelina Nuculoides Mollusca Bivalvia Semelidae 

551535060200 Semele Bellastriata Mollusca Bivalvia Semelidae 

551535060300 Semele Sp. A Mollusca Bivalvia Semelidae 

551537020100 Mytilopsis Leucophaeata Mollusca Bivalvia 

551545010100 Polymesoda Caroliniana Mollusca Bivalvia 

551545020100 Corbicula Fluminea Mollusca Bivalvia



551546010000 Sphaerium Mollusca Bivalvia Sphaeriidae 

551547000000 49 VENERIDAE Mollusca Bivalvia Veneridae 

551547000001 Veneridae Sp. A Mollusca Bivalvia Veneridae 

551547010300 Transennella Conradina Mollusca Bivalvia Veneridae 

551547010400 Transenella Stimpsoni Mollusca Bivalvia Veneridae 

551547090100 Dosinia Discus Mollusca Bivalvia Veneridae 

551547090200 Dosinia Eleganis Mollusca Bivalvia Veneridae 

551547100100 Cyclinella Tenuis Mollusca Bivalvia Veneridae 

551547110000 Mercenaria Mollusca Bivalvia Veneridae 

551547120000 Pitar Sp. Mollusca Bivalvia Veneridae 

551547120200 Pitar Cordatus Mollusca Bivalvia Veneridae 

551547120400 Pitar Fulminatus Mollusca Bivalvia Veneridae 

551547130100 Gemma Gemma Mollusca Bivalvia Veneridae 

551547140100 Agriopoma Texasianum Mollusca Bivalvia Veneridae 

551547150200 Timoclea Grus Mollusca Bivalvia Veneridae 

551547150300 Chione Cancellata Mollusca Bivalvia Veneridae 

551547180200 Macrocallista Nimbosa Mollusca Bivalvia Veneridae 

551547230100 Anomalocardia Auberiana Mollusca Bivalvia Veneridae 

551547280100 Parastarte Triquetra Mollusca Bivalvia Veneridae 

551701040300 Sphenia Antillensis Mollusca Bivalvia 

551702020100 Corbula Contracta Mollusca Bivalvia Corbulidae 

551702020200 Caryocorbula Barrattiana Mollusca Bivalvia Corbulidae 

551702020500 Corbula Swiftiana Mollusca Bivalvia Corbulidae 

551801030100 Cyrtopleura Costata Mollusca Bivalvia 

551801061000 Martesia Striata Mollusca Bivalvia 

552002010600 Pandora Trilineata Mollusca Bivalvia 

552005020601 Lyonsia Floridana Mollusca Bivalvia Lyonsiidae 

552007010600 Periploma Margaritaceum Mollusca Bivalvia 

552008000001 50 THRACIIDAE Mollusca Bivalvia 

552008010200 Asthenothaerus Hemphilli Mollusca Bivalvia 

552008019800 Asthenothaerus Sp. B Mollusca Bivalvia



552008019900 Asthenothaerus Sp. A Mollusca Bivalvia 

560001010400 Graptacme Eborea Mollusca Scaphopoda 

560001011200 Antalus Antillarum Mollusca Scaphopoda 

560001012600 Graptacme Calamus Mollusca Scaphopoda 

560101010000 Dentalium Sp. Mollusca Scaphopoda 

560101010500 Dentalium Texasianum (=American Mollusca Scaphopoda 

560101010600 Dentalium Laqueatum Mollusca Scaphopoda 

580201010100 Limulus Polyphemus 

592201000000 Hydracarina 

593400000000 Hydrachnellae 

600000000000 Pycnogonida 

613000000000 Cirripedia Arthropoda Crustacea Cirripedia 

613402012100 Balanus Venustus Arthropoda Crustacea Cirripedia 

614510100000 Nebalia Arthropoda Crustacea 

615301210000 Mysidopsis Spp. Arthropoda Crustacea Mysidacea 

615301210100 Americamysis Bigelowi Arthropoda Crustacea Mysidacea 

615301210300 Mysidopsis Almyra Arthropoda Crustacea Mysidacea 

615301210500 Mysidopsis Furca Arthropoda Crustacea Mysidacea 

615301260000 Bowmaniella Sp. Arthropoda Crustacea Mysidacea 

615301260100 Bowmaniella Brasiliensis Arthropoda Crustacea Mysidacea 

615301260300 Bowmaniella Floridana Arthropoda Crustacea Mysidacea 

615301270200 Taphromysis Bowmani Arthropoda Crustacea Mysidacea 

615301290000 Brasilomysis Sp. Arthropoda Crustacea Mysidacea 

615301290100 Brasilomysis Castroi Arthropoda Crustacea Mysidacea 

615303150000 Americamysis Spp. Arthropoda Crustacea Mysidacea 

615303150100 Americamysis Alleni Arthropoda Crustacea Mysidacea 

615303150200 Americamysis Bahia Arthropoda Crustacea Mysidacea 

615303150300 Americamysis Stucki Arthropoda Crustacea Mysidacea 

615400000001 51 CUMACEA Arthropoda Crustacea Cumacea 

615404011000 Leucon Americanus Arthropoda Crustacea Cumacea 

615404019900 Leucon Sp. A Arthropoda Crustacea Cumacea



615405080000 Oxyurostylis Spp. Arthropoda Crustacea Cumacea 

615405089800 Oxyurostylis Smithi Arthropoda Crustacea Cumacea 

615405089900 Oxyurostylis Lecroyae Arthropoda Crustacea Cumacea 

615408000001 Nannastacidae Arthropoda Crustacea Cumacea 

6154080103cf Cumella Cf. Garrityi Arthropoda Crustacea Cumacea 

615408020100 Almyracuma Proximoculi Arthropoda Crustacea Cumacea 

615409020000 Cyclaspis Spp. Arthropoda Crustacea Cumacea 

615409020100 Cyclaspis Pustulata Arthropoda Crustacea Cumacea 

6154090202cf Cyclaspis Cf. Varians Arthropoda Crustacea Cumacea 

615409029700 Cyclaspis Sp. C Arthropoda Crustacea Cumacea 

615409029800 Cyclaspis Sp. B Arthropoda Crustacea Cumacea 

615409029900 Cyclaspis Sp. D Arthropoda Crustacea Cumacea 

615603019900 Apseudes Sp. A Arthropoda Crustacea Isopoda 

615603020100 Halmyrapseudes Bahamensis Arthropoda Crustacea Isopoda 

615606019900 Kalliapseudes Sp. A Arthropoda Crustacea Isopoda 

615701010400 Sinelobus Stanfordi Arthropoda Crustacea Isopoda 

615715020000 Leptochelia Sp. Arthropoda Crustacea Isopoda 

615715021200 Leptochelia (Hargeria) Rapax Arthropoda Crustacea Isopoda 

616000000000 52 ISOPODA Arthropoda Crustacea Isopoda 

616001000000 ANTHURIDEA Arthropoda Crustacea Isopoda 

616001020100 Cyathura Polita Arthropoda Crustacea Isopoda 

616001070100 Xenanthura Brevitelson Arthropoda Crustacea Isopoda 

616001110100 Horolanthura Irpex Arthropoda Crustacea Isopoda 

616001200100 Amakusanthura Magnifica Arthropoda Crustacea Isopoda 

616100000000 53 FLABELLIFERA Arthropoda Crustacea Isopoda 

616101010800 Cirolana Parva Arthropoda Crustacea Isopoda 

616101020800 Eurydice Personata Arthropoda Crustacea Isopoda 

616102000000 54 SPHAEROMATIDAE Arthropoda Crustacea Isopoda 

616102020200 Paracerceis Caudata Arthropoda Crustacea Isopoda 

616102070200 Sphaeroma Quadridentata Arthropoda Crustacea Isopoda 

616102080200 Cassidinidea Ovalis Arthropoda Crustacea Isopoda



616102110100 Harrieta Faxoni Arthropoda Crustacea Isopoda 

616103010100 Serolis Mgrayi Arthropoda Crustacea Isopoda 

616202060100 Erichsonella Attenuata Arthropoda Crustacea Isopoda 

616202060200 Erichsonella Filiformis Arthropoda Crustacea Isopoda 

616202070300 Edotia Triloba Arthropoda Crustacea Isopoda 

616300000000 55 ASELLOTA Arthropoda Crustacea Isopoda 

616312000000 Munnidae? Arthropoda Crustacea Isopoda 

616312010000 Munna Sp. N. Arthropoda Crustacea Isopoda 

616312040100 Uromunna Hayesi Arthropoda Crustacea Isopoda 

616312040200 Uromunna Reynoldsi Arthropoda Crustacea Isopoda 

616501000000 Cryptoniscidae? Arthropoda Crustacea Isopoda 

616900000000 56 AMPHIPODA Arthropoda Crustacea Gammaridea 

616900000002 GAMMARIDEA Arthropoda Crustacea Gammaridea 

616900000003 Gammaridea A [Dam] Arthropoda Crustacea Gammaridea 

616900000004 Gammaridea B [Dam] Arthropoda Crustacea Gammaridea 

616902000000 57 AMPELISCIDAE Arthropoda Crustacea Gammaridea Ampeliscidae 

616902010000 Ampelisca Spp. Arthropoda Crustacea Gammaridea Ampeliscidae 

616902010800 Ampelisca Abdita Arthropoda Crustacea Gammaridea Ampeliscidae 

616902010900 Ampelisca Vadorum Arthropoda Crustacea Gammaridea Ampeliscidae 

616902011100 Ampelisca Agassizi Arthropoda Crustacea Gammaridea Ampeliscidae 

616902012300 Ampelisca Holmesi Arthropoda Crustacea Gammaridea Ampeliscidae 

616902013900 Ampelisca Bicarinata Arthropoda Crustacea Gammaridea Ampeliscidae 

616902019700 Ampelisca Sp. C Arthropoda Crustacea Gammaridea Ampeliscidae 

616902019800 Ampelisca Sp. B Arthropoda Crustacea Gammaridea Ampeliscidae 

616902019900 Ampelisca Sp. A Arthropoda Crustacea Gammaridea Ampeliscidae 

616902020000 Ampelisca Abdita/Vadorum Arthropoda Crustacea Gammaridea Ampeliscidae 

616903000000 58 AMPHILOCIDAE Arthropoda Crustacea Gammaridea Amphilocidae 

616903020100 Amphilochus Neopolitanus Arthropoda Crustacea Gammaridea Amphilocidae 

6169030203cf Amphilochus Cf. Casahoya Arthropoda Crustacea Gammaridea Amphilocidae 

616903029800 Amphilochus B Arthropoda Crustacea Gammaridea Amphilocidae 

616903029900 Amphilochus A Arthropoda Crustacea Gammaridea Amphilocidae



616903040000 Gitanopsis Sp. Arthropoda Crustacea Gammaridea Amphilocidae 

616903040300 Gitanopsis Laguna Arthropoda Crustacea Gammaridea Amphilocidae 

616904000001 59 AMPITHOIDAE Arthropoda Crustacea Gammaridea Ampithoidae 

616904010000 Ampithoe Sp. Arthropoda Crustacea Gammaridea Ampithoidae 

616904011500 Ampithoe Longimana Arthropoda Crustacea Gammaridea Ampithoidae 

616904011600 Ampithoe Valida Arthropoda Crustacea Gammaridea Ampithoidae 

616904012100 Ampithoe ?Ramondi Arthropoda Crustacea Gammaridea Ampithoidae 

616904020100 Cymadusa Compta Arthropoda Crustacea Gammaridea Ampithoidae 

616906000001 60 AORIDAE Arthropoda Crustacea Gammaridea Aoridae 

616906030300 Globosolembos Smithi Arthropoda Crustacea Gammaridea Aoridae 

616906030600 Bemlos Brunneamaculatus Arthropoda Crustacea Gammaridea Aoridae 

616906030601 Bemlos Brunneamaculatus Mackinn Arthropoda Crustacea Gammaridea Aoridae 

616906030602 Bemlos Brunneamaculatus Setosus Arthropoda Crustacea Gammaridea Aoridae 

616906030700 Bemlos Unifasciatus Arthropoda Crustacea Gammaridea Aoridae 

616906030800 Bemlos Spinicarpus Arthropoda Crustacea Gammaridea Aoridae 

616906031300 Bemlos Setosus Arthropoda Crustacea Gammaridea Aoridae 

6169060404cf Paramicrodeutopus Cf. Myersi Arthropoda Crustacea Gammaridea Aoridae 

616906120100 Rudilemboides Naglei Arthropoda Crustacea Gammaridea Aoridae 

616906160000 Bemlos Sp. Arthropoda Crustacea Gammaridea Aoridae 

616906160100 Bemlos Rectangulatus Arthropoda Crustacea Gammaridea Aoridae 

616906160200 Bemlos Unicornis Arthropoda Crustacea Gammaridea Aoridae 

616907010100 Argissa Hamatipes Arthropoda Crustacea Gammaridea 

616910010100 Batea Catharinensis Arthropoda Crustacea Gammaridea Bateidae 

616910010300 Batea Cuspidata Arthropoda Crustacea Gammaridea Bateidae 

616915000000 61 COROPHIIDAE Arthropoda Crustacea Gammaridea Corophiidae 

616915010000 Cerapus Spp. Arthropoda Crustacea Gammaridea Corophiidae 

616915010200 Cerapus Sp. C (="Tubularis") Arthropoda Crustacea Gammaridea Corophiidae 

616915019600 Cerapus Sp. D Arthropoda Crustacea Gammaridea Corophiidae 

616915019900 Cerapus Sp. A Arthropoda Crustacea Gammaridea Corophiidae 

616915020100 Monocorophium Acherusicum Arthropoda Crustacea Gammaridea Corophiidae 

616915020700 Monocorophium Tuberculatum Arthropoda Crustacea Gammaridea Corophiidae



6169150214cf Laticorophium Cf. Baconi Arthropoda Crustacea Gammaridea Corophiidae 

616915021500 Apocorophium Louisianum Arthropoda Crustacea Gammaridea Corophiidae 

616915021800 Americorophium Ellisi Arthropoda Crustacea Gammaridea Corophiidae 

616915030200 Erichthonius Brasiliensis Arthropoda Crustacea Gammaridea Corophiidae 

616915090100 Grandidierella Bonnieroides Arthropoda Crustacea Gammaridea Corophiidae 

616920120000 62 PONTOGENEIDAE Arthropoda Crustacea Gammaridea 

616920121100 Pontogenia Bartschi Arthropoda Crustacea Gammaridea 

616920121101 Tethygeneia Longleyi Arthropoda Crustacea Gammaridea 

616921000000 GAMMARIDAE Arthropoda Crustacea Gammaridea Melitidae 

616921029900 Ceradocus Sp. A Arthropoda Crustacea Gammaridea Melitidae 

616921030100 Elasmopus Laevis Arthropoda Crustacea Gammaridea Melitidae 

616921030400 Elasmopus Procellimanus Arthropoda Crustacea Gammaridea Melitidae 

616921070800 Gammarus Palustris Arthropoda Crustacea Gammaridea Melitidae 

616921070900 Mucrogammarus Mucronatus Arthropoda Crustacea Gammaridea Melitidae 

616921080000 Maera Sp. Arthropoda Crustacea Gammaridea Melitidae 

616921089900 Maera Sp. N Arthropoda Crustacea Gammaridea Melitidae 

616921100000 Melita Sp. Arthropoda Crustacea Gammaridea Melitidae 

616921100700 Dulichiella Appendiculata Arthropoda Crustacea Gammaridea Melitidae 

616921101400 Melita Elongata Arthropoda Crustacea Gammaridea Melitidae 

616922060700 Acanthohaustorius Uncinus Arthropoda Crustacea Gammaridea Haustoriidae 

616922139900 Pseudohaustorius Sp. A Arthropoda Crustacea Gammaridea Haustoriidae 

616923040100 Hyalella Azteca Arthropoda Crustacea Gammaridea Hyalellidae 

616926000000 63 PHOTIDAE Arthropoda Crustacea Gammaridea Photidae 

616926020000 Photis Sp. Arthropoda Crustacea Gammaridea Photidae 

616926021400 Photis Sp. C Arthropoda Crustacea Gammaridea Photidae 

616926090000 Microprotopus Arthropoda Crustacea Gammaridea Photidae 

616926090100 Microprotopus Raneyi Arthropoda Crustacea Gammaridea Photidae 

616926090200 Microprotopus Shoemakeri Arthropoda Crustacea Gammaridea Photidae 

616932010100 Leucothoe Spinicarpa Arthropoda Crustacea Gammaridea 

616933030100 Listriella Barnardi Arthropoda Crustacea Gammaridea Liljeborgiidae 

616934000000 64 LYSIANASSIDAE Arthropoda Crustacea Gammaridea Lysianassidae



616934009800 Shoemakerella (Lysian.Sp.B) Arthropoda Crustacea Gammaridea Lysianassidae 

616934530900 Shoemakerella Lowreyi Arthropoda Crustacea Gammaridea Lysianassidae 

616934970000 Lysianassidae Genus C Arthropoda Crustacea Gammaridea Lysianassidae 

616935000000 65 MELPHIDIPPIDAE Arthropoda Crustacea Gammaridea 

6169350301cf Gibberosus Cf. Myersi Arthropoda Crustacea Gammaridea 

616937000000 66 OEDICEROTIDAE Arthropoda Crustacea Gammaridea Oedicerotidae 

616937082300 Monoculodes Nyei Arthropoda Crustacea Gammaridea Oedicerotidae 

616937140100 Synchelidium Americanum Arthropoda Crustacea Gammaridea Oedicerotidae 

616942000000 67 PHOXOCEPHALIDAE Arthropoda Crustacea Gammaridea 

616942140300 Metharpinia Floridana Arthropoda Crustacea Gammaridea 

616942159900 Rhepoxynius Sp. A Arthropoda Crustacea Gammaridea 

616942190100 Eobrolgus Spinosus Arthropoda Crustacea Gammaridea 

616942210100 Eudevenopus Honduranus Arthropoda Crustacea Gammaridea 

616948000000 68 STENOTHOIDAE Arthropoda Crustacea Gammaridea Stenothoidae 

616948070300 Parametopella Texensis Arthropoda Crustacea Gammaridea Stenothoidae 

616948079900 Parametopella Sp. A Arthropoda Crustacea Gammaridea Stenothoidae 

616948100000 Stenothoe Sp. Arthropoda Crustacea Gammaridea Stenothoidae 

616948100100 Stenothoe Gallensis Arthropoda Crustacea Gammaridea Stenothoidae 

616948100200 Stenothoe Minuta Arthropoda Crustacea Gammaridea Stenothoidae 

616948100900 Stenothoidae A Arthropoda Crustacea Gammaridea Stenothoidae 

616948109900 Stenothoe Sp. A Arthropoda Crustacea Gammaridea Stenothoidae 

616950050000 Tiron Sp. Arthropoda Crustacea Gammaridea Synopiidae 

616950050500 Tiron Tropakis Arthropoda Crustacea Gammaridea Synopiidae 

616950050600 Tiron Triocellatus Arthropoda Crustacea Gammaridea Synopiidae 

617010000000 69 CAPRELLIDEA Arthropoda Crustacea 

617010000010 Caprellidae-Unid. Arthropoda Crustacea 

617101020200 Deutella Incerta Arthropoda Crustacea Caprellidea 

617101072700 Caprella Penantis Arthropoda Crustacea Caprellidea 

617101090000 Paracaprella Sp. Arthropoda Crustacea Caprellidea 

617101090100 Paracaprella Tenuis Arthropoda Crustacea Caprellidea



617101090200 Paracaprella Pusilla Arthropoda Crustacea Caprellidea 

617101150100 Hemiaegina Minuta Arthropoda Crustacea Caprellidea 

617201011600 Ingolfiellidia Fuscina Arthropoda Crustacea Ingolfiellidea 

617201019900 Ingolfiellidia Sp. A Arthropoda Crustacea Ingolfiellidea 

617202000000 Ingolfiellidea Arthropoda Crustacea Ingolfiellidea 

617600000000 70 DECAPODA Arthropoda Crustacea Decapoda 

617700000000 PENAEOIDEA Arthropoda Crustacea Decapoda Penaeidea 

617701010200 Penaeus Duorarum Arthropoda Crustacea Decapoda Penaeidea 

617701020000 Trachypenaeus Arthropoda Crustacea Decapoda Penaeidea 

617701020100 Trachypenaeus Constrictus Arthropoda Crustacea Decapoda Penaeidea 

617701030000 Metapenaeopsis Sp. Arthropoda Crustacea Decapoda Penaeidea 

617701040300 Sicyonia Typica Arthropoda Crustacea Decapoda Penaeidea 

617701040400 Sicyonia Laevigata Arthropoda Crustacea Decapoda Penaeidea 

617704010000 Sicyonia Sp. Arthropoda Crustacea Decapoda Penaeidea 

617900000000 71 CARIDEA Arthropoda Crustacea Decapoda Caridea 

617905020100 Leptochela Serratorbita Arthropoda Crustacea Decapoda Caridea 

617905020200 Leptochela Bermudensis Arthropoda Crustacea Decapoda Caridea 

617911000000 72 PALAEMONIDAE Arthropoda Crustacea Decapoda Caridea 

617911040100 Periclimenes Americanus Arthropoda Crustacea Decapoda Caridea 

617911040300 Periclimenes Longicaudatus Arthropoda Crustacea Decapoda Caridea 

617911050000 Pontonia Sp. Arthropoda Crustacea Decapoda Caridea 

617914000000 73 ALPHEIDAE Arthropoda Crustacea Decapoda Caridea 

617914010000 Alpheus Sp. Arthropoda Crustacea Decapoda Caridea 

617914010100 Alpheus Heterochaelis Arthropoda Crustacea Decapoda Caridea 

617914010200 Alpheus Normanni Arthropoda Crustacea Decapoda Caridea 

617914011100 Alpheus Angulatus Arthropoda Crustacea Decapoda Caridea 

617914014300 Alpheus Armillatus Arthropoda Crustacea Decapoda Caridea 

617914030000 Automate Sp. Arthropoda Crustacea Decapoda Caridea 

617914030100 Automate Evermanni Arthropoda Crustacea Decapoda Caridea 

617914030300 Automate Rectifrons Arthropoda Crustacea Decapoda Caridea 

617914030400 Automate Dolicognatha Arthropoda Crustacea Decapoda Caridea



617914039900 Automate Sp. A Arthropoda Crustacea Decapoda Caridea 

617914060000 Synalpheus Sp. Arthropoda Crustacea Decapoda Caridea 

617914061600 Synalpheus Normanni Arthropoda Crustacea Decapoda Caridea 

617915010100 Ogyrides Alphaerostris Arthropoda Crustacea Decapoda Caridea 

617916000000 74 HIPPOLYTIDAE Arthropoda Crustacea Decapoda Caridea 

617916010000 Hippolyte Sp. Arthropoda Crustacea Decapoda Caridea 

617916010300 Hippolyte Pleuracantha Arthropoda Crustacea Decapoda Caridea 

617916010400 Hippolyte Zostericola Arthropoda Crustacea Decapoda Caridea 

617916060100 Latreutes Fucorum Arthropoda Crustacea Decapoda Caridea 

617916060200 Latreutes Parvulus Arthropoda Crustacea Decapoda Caridea 

617916110000 Lysmata Sp. Arthropoda Crustacea Decapoda Caridea 

617916120100 Tozeuma Carolinense Arthropoda Crustacea Decapoda Caridea 

617916140000 Thor Sp. Arthropoda Crustacea Decapoda Caridea 

617917000000 75 PROCESSIDAE Arthropoda Crustacea Decapoda Caridea 

617917010000 Processa Sp. Arthropoda Crustacea Decapoda Caridea 

617917010100 Processa Hemphilli Arthropoda Crustacea Decapoda Caridea 

617917010200 Processa Bermudensis Arthropoda Crustacea Decapoda Caridea 

617917030100 Ambidexter Symmetricus Arthropoda Crustacea Decapoda Caridea 

618301000000 76 THALASSINIDEA Arthropoda Crustacea Decapoda Anomura 

618304000000 CALLIANASSIDAE Arthropoda Crustacea Decapoda Anomura Callianassidae 

618304040001 Callichirinae Sp. Arthropoda Crustacea Decapoda Anomura Callianassidae 

618305000000 PAGUROIDEA Arthropoda Crustacea Decapoda Anomura 

618306000001 PAGURIDAE Arthropoda Crustacea Decapoda Anomura Paguridae 

618306000002 DIOGENIDAE Arthropoda Crustacea Decapoda Anomura Paguridae 

618306010000 Paguristes Sp. Arthropoda Crustacea Decapoda Anomura Paguridae 

618306010400 Paguristes Hummi Arthropoda Crustacea Decapoda Anomura Paguridae 

618306011600 Paguristes Nr. Tortugae Arthropoda Crustacea Decapoda Anomura Paguridae 

618306020000 Pagurus Sp. Arthropoda Crustacea Decapoda Anomura Paguridae 

618306024600 Pagurus Stimpsoni Arthropoda Crustacea Decapoda Anomura Paguridae 

618306024700 Pagurus Gymnodactylus Arthropoda Crustacea Decapoda Anomura Paguridae 

618306025100 Pagurus Maclaughlinae Arthropoda Crustacea Decapoda Anomura Paguridae



618312000000 77 PORCELLANIDAE Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618312010000 Petrolisthes Sp. Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618312010300 Petrolisthes Armatus Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618312020000 Pachycheles Sp. Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618312030100 Euceramus Praelongus Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618312040100 Polyonyx Gibbesi Arthropoda Crustacea Decapoda Anomura Porcellanidae 

618313020100 Albunea Paretii Arthropoda Crustacea Decapoda Anomura 

618317010000 Upogebia Arthropoda Crustacea Decapoda Anomura 

618317010200 Upogebia Affinis Arthropoda Crustacea Decapoda Anomura 

618400000000 78 BRACHYURA Arthropoda Crustacea Decapoda Brachyura 

618400009900 Brachyura A Arthropoda Crustacea Decapoda Brachyura 

618502010100 Hypoconcha Arcuata Arthropoda Crustacea Decapoda 

618602030000 Osachila Sp. Arthropoda Crustacea Decapoda 

618603010400 Persephona Mediterranea Arthropoda Crustacea Decapoda 

618603030300 Iliacantha Subglobosa Arthropoda Crustacea Decapoda 

618701090100 Libinia Dubia Arthropoda Crustacea Decapoda 

618701130100 Pelia Mutica Arthropoda Crustacea Decapoda 

618701320000 Pitho Sp. Arthropoda Crustacea Decapoda 

618701320100 Pitho Lherminieri Arthropoda Crustacea Decapoda 

618701320200 Pitho Laevigata Arthropoda Crustacea Decapoda 

618701320300 Pitho Anisodon Arthropoda Crustacea Decapoda 

618701320400 Pitho Aculeata Arthropoda Crustacea Decapoda 

618702080100 Heterocrypta Granulata Arthropoda Crustacea Decapoda 

618901060000 Portunus Sp. Arthropoda Crustacea Decapoda 

618901060300 Portunus Spinicarpus Arthropoda Crustacea Decapoda 

618901060800 Portunus Ordwayi Arthropoda Crustacea Decapoda 

618902000001 79 PANOPEIDAE Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902050000 Eurypanopeus Sp. Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902060000 Hexapanopeus Sp. Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902060100 Hexapanopeus Angustifrons Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902080000 Panopeus Sp. Arthropoda Crustacea Decapoda Brachyura Panopeidae



618902080100 Panopeus Herbstii Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902080500 Panopeus Bermudensis Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902080600 Panopeus Rugosus? Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902090100 Rhithropanopeus Harrisii Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902130100 Menippe Mercenaria Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902140000 Pilumnus Sp. Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902420000 Dyspanopeus Sp. Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618902420200 Dyspanopeus Texanus Arthropoda Crustacea Decapoda Brachyura Panopeidae 

618905000001 80 GONEPLACIDAE Arthropoda Crustacea Decapoda 

618906000000 PINNOTHERIDAE Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906010000 Dissodactylus Sp. Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906010100 Dissodactylus Mellitae Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906020000 Pinnotheres Sp. Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906020100 Tumidotheres Maculatus Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906040000 Pinnixa Spp. Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906040500 Pinnixa Chaetopterana Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906040600 Pinnixa Cylindrica Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906040800 Pinnixa Retinens Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906040900 Pinnixa Sayana Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906041200 Pinnixa Cf. Chacei Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

6189060413cf Pinnixa Cf. Pearsei Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

6189060414cf Pinnixa Cf. Floridana Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906049800 Pinnixa B Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906049900 Pinnixa A Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906050100 Pinnixa D Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906050400 Pinnixa G Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

618906050500 Pinnixa E Arthropoda Crustacea Decapoda Brachyura Pinnotheridae 

619100000000 81 STOMATOPODA Arthropoda Crustacea Decapoda 

619101000000 SQUILLIDAE Arthropoda Crustacea Decapoda 

619101010100 Squilla Cf. Empusa Arthropoda Crustacea Decapoda 

619105000000 82 NANNOSQUILLIDAE Arthropoda Crustacea Decapoda



619105010000 Nannosquilla Sp. Arthropoda Crustacea Decapoda 

619105010100 Nannosquilla Taylori Arthropoda Crustacea Decapoda 

621602000000 BATEIDAE Arthropoda Insecta Ephemeroptera 

621602130000 Pseudocloeon Sp. Arthropoda Insecta Ephemeroptera 

621602180000 Neocloeon Sp. Arthropoda Insecta Ephemeroptera 

621602990000 Callibaetis Sp. Arthropoda Insecta Ephemeroptera 

621802000000 83 CAENIDAE Arthropoda Insecta Ephemeroptera 

621802010000 Bracycercus Sp. Arthropoda Insecta Ephemeroptera 

621802020000 Caenis Sp. Arthropoda Insecta Ephemeroptera 

621802030100 Cercobrachys Etowah Arthropoda Insecta Ephemeroptera 

630504020000 Peltodytes Sp. Arthropoda Insecta Coleoptera 

631604020000 Dubiraphia Sp. Arthropoda Insecta Coleoptera 

641800000000 84 TRICHOPTERA Arthropoda Insecta Trichoptera 

641803030000 Cyrnellus Sp. Arthropoda Insecta Trichoptera 

641803030100 Cyrnellus Fraternus Arthropoda Insecta Trichoptera 

641812010000 Oecetis Sp. Arthropoda Insecta Trichoptera 

641812010100 Oecetis Nocturna Arthropoda Insecta Trichoptera 

641812010200 Oecetis Inconspicus Arthropoda Insecta Trichoptera 

650503010000 Chaoborus Punctipennis Arthropoda Insecta Diptera Culicidae 

650504050000 Probezzia Sp. Arthropoda Insecta Diptera Heleidae 

650504080000 Dasyhelea Sp. Arthropoda Insecta Diptera Heleidae 

650504090000 Mallochohela Sp.? Arthropoda Insecta Diptera Heleidae 

650504100000 Bezzia/Palmpomyia Arthropoda Insecta Diptera Heleidae 

650508000000 85 CHIRONOMIDAE Arthropoda Insecta Diptera Chironomidae 

650508020800 Coelotanypus Scapularis Arthropoda Insecta Diptera Chironomidae 

650508030000 Stenochironomus Arthropoda Insecta Diptera Chironomidae 

650508040000 Procladius Sp. Arthropoda Insecta Diptera Chironomidae 

650508049900 Procladius (Holotanypus) Sp. 1 Arthropoda Insecta Diptera Chironomidae 

650508260000 Cricotopus Sp. Arthropoda Insecta Diptera Chironomidae 

650508310000 Tanytarsus Sp. Arthropoda Insecta Diptera Chironomidae 

650508310500 Tanytarsus E Arthropoda Insecta Diptera Chironomidae



650508310700 Tanytarsus G Arthropoda Insecta Diptera Chironomidae 

650508311500 Tanytarsus O Arthropoda Insecta Diptera Chironomidae 

650508311700 Tanytarsus Q Arthropoda Insecta Diptera Chironomidae 

650508330000 Chironomus Sp. Arthropoda Insecta Diptera Chironomidae 

650508360000 Cryptochironomus Sp. Arthropoda Insecta Diptera Chironomidae 

650508370000 Polypedilum Sp. Arthropoda Insecta Diptera Chironomidae 

650508371100 Polypedilum Convictum Arthropoda Insecta Diptera Chironomidae 

650508371700 Polypedilum Halterale Grp. Arthropoda Insecta Diptera Chironomidae 

650508372300 Polypedilum Simulans Arthropoda Insecta Diptera Chironomidae 

650508379900 Polypedilum Scalaneum Group Arthropoda Insecta Diptera Chironomidae 

650508380000 Pseudochironomus Sp. Arthropoda Insecta Diptera Chironomidae 

650508390000 Glyptotendipes Sp. Arthropoda Insecta Diptera Chironomidae 

650508430000 Cladotanytarsus Sp. Arthropoda Insecta Diptera Chironomidae 

650508430300 Cladotanytarsus Daviesi Arthropoda Insecta Diptera Chironomidae 

650508440000 Ablabesmyia Sp. Arthropoda Insecta Diptera Chironomidae 

650508441400 Ablabesmyia Mallochi Arthropoda Insecta Diptera Chironomidae 

650508442000 Ablabesmyia Rhamphe Arthropoda Insecta Diptera Chironomidae 

650508442100 Ablabesmyia (Karelia) Sp. Arthropoda Insecta Diptera Chironomidae 

650508450900 Dicrotendipes Neomodestus Arthropoda Insecta Diptera Chironomidae 

650508451200 Dicrotendipes Simpsoni Arthropoda Insecta Diptera Chironomidae 

650508461000 Nanocladius Distinctus Arthropoda Insecta Diptera Chironomidae 

650508570100 Djalmabatista Pulchra Arthropoda Insecta Diptera Chironomidae 

650508580100 Fissimentum Sp. A Arthropoda Insecta Diptera Chironomidae 

650508590000 Stempellina Sp. Arthropoda Insecta Diptera Chironomidae 

650508920000 Pagastiella Sp. Arthropoda Insecta Diptera Chironomidae 

650508930000 Axarus Sp. Arthropoda Insecta Diptera Chironomidae 

650508940100 Asheum Beckae Arthropoda Insecta Diptera Chironomidae 

650508950100 Goeldichironomus Amazonicus Arthropoda Insecta Diptera Chironomidae 

650508960100 Paracladopelma Cf. Doris Arthropoda Insecta Diptera Chironomidae 

650508970000 Lopescaldius Sp. Arthropoda Insecta Diptera Chironomidae 

650508980000 Cryptotendipes Sp. Arthropoda Insecta Diptera Chironomidae 

650508990000 Chironomini Genus A Arthropoda Insecta Diptera Chironomidae



720000000000 86 SIPUNCULA Sipuncula 

720000009500 Sipuncula Sp. A Sipuncula 

720000009600 Sipuncula Sp. B Sipuncula 

720000009700 Sipuncula Sp. C Sipuncula 

720002040100 Phascolion Crypta Sipuncula 

720003000000 Aspidosiphonidae Sipuncula 

740000000000 Priapula Sp. 

740001010000 Priapulus Sp. 

770001020000 Phoronis Sp. Phoronida 

770001020300 Phoronis ?Architecta Phoronida 

770001029600 Phoronis D Phoronida 

770001029700 Phoronis A Phoronida 

770001029800 Phoronis C Phoronida 

770001029900 Phoronida B Phoronida 

780000000000 BRYOZOA 

780000009300 Bryozoa G 

780000009400 Bryozoa F 

780000009500 Bryozoa E 

780000009600 Bryozoa D 

781504010000 Membranipora 

781515020000 Discoporella Sp. 

800201010100 Glottidia Pyramidata Brachiopoda 

810400000000 87 ASTEROIDEA Echinodermata 

812000000000 OPHIUROIDEA Echinodermata 

812701100100 Ophiolepis Elegans Echinodermata 

812705010100 Ophioderma Brevispinum Echinodermata 

812902020100 Hemipholis Elongata Echinodermata 

812902030100 Ophiactis Savignyi Echinodermata 

812903000000 AMPHIUROIDEA Echinodermata 

812903010600 Amphiodia Pulchella Echinodermata 

8129030117nr Amphiodia Nr. Riisei Echinodermata



812903020000 Amphipholis Sp. Echinodermata 

812903020200 Amphipholis Squamata Echinodermata 

812903020500 Amphipholis Gracillima Echinodermata 

812903060000 Ophiophragmus Sp. Echinodermata 

812903060300 Ophiophragmus Wurdemanii Echinodermata 

812903060400 Ophiophragmus Pulcher Echinodermata 

812903060600 Ophiophragmus Filograneus Echinodermata 

812903060900 Ophiphragmus Brachyactus Echinodermata 

812903090000 Amphioplus Sp. Echinodermata 

812903090100 Amphioplus Abditus Echinodermata 

812903090400 Amphioplus Thrombodes Echinodermata 

812903090500 Amphioplus Sepultus Echinodermata 

812903091100 Amphioplus Cuneatus Echinodermata 

812903099900 Amphioplus A Echinodermata 

812903101100 Amphiura Flexuosa Echinodermata 

812903120000 Micropholis Sp. Echinodermata 

812903120100 Amphipholis Atra Echinodermata 

812903129900 Amphipholis Sp. A Echinodermata 

812904010200 Ophiothrix Angulata Echinodermata 

813600000000 88 ECHINOIDEA Echinodermata 

815504010100 Mellita Tenuis Echinodermata 

817000000000 89 HOLOTHUROIDEA Echinodermata 

817000000002 Holothuroidea B Echinodermata 

817000000003 Holothuroidea C Echinodermata 

817203000000 Psolidae A Echinodermata 

817400000000 Aspidochirotacea Echinodermata 

817401010100 Eostichopus Regalis Echinodermata 

817801000001 Synaptidae Sp. Echinodermata 

817801009500 Synaptidae A Echinodermata 

817801009700 Synaptidae C Echinodermata 

817801020000 Leptosynapta Echinodermata



820100000000 90 ENTEROPNEUSTA 

820100000001 Enteropneusta A 

820100000002 Enteropneusta B 

820100000003 Enteropneusta C 

820101030300 Saccoglossus A 

821010201000 Stereobalanus Canadensis 

840100000000 ASCIDIACEA 

850001010000 Branchiostoma Floridae

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7.0 Adult and Juvenile Fish 

The primary objective of this HBMP element is to develop comprehensive, long-term 

measurements of species composition (to the lowest practical identifiable level), 

abundance and distribution of adult and juvenile fishes within each of the four major 

reporting units. This section describes activities being conducted by the Florida Fish and 

Wildlife Conservation Commission’s Fisheries-Independent Monitoring (FIM) section of 

the Fish and Wildlife Research Institute (FWRI), formerly known as the Florida Marine 

Research Institute (FMRI), in St. Petersburg. 

7.1 Project Management 

The overall general organization of the adult and juvenile fishes HBMP monitoring 

element is outlined in Figure 7.1. There are three critical points for QA/QC feedback: at 

the Project Manager position; at the Sampling Operations Manager position; and at the 

Data Management position. The Project Manager is ultimately responsible for all 

internal QA/QC aspects of the field sampling, species identification and data 

management facets of the sampling program. It is the initial responsibility of the 

Sampling Operations Manager to oversee the implementation of all QA/QC protocols 

associated with the collection of field data and species identification. 

7.2 HBMP Adult and Juvenile Fish Monitoring 

Sampling will be conducted monthly as outlined in Table 7.1, and the data submitted to 

the primary contractor (PBS&J) on a quarterly basis. Each of the tributaries (Alafia, 

Palm and Hillsborough Rivers) and bays (McKay Bay and Hillsborough Bay) are 

considered separate reporting units. Each of the tributaries has been subdivided into 

relatively equal length strata, ensuring that the entire salinity gradient is sampled each 

month. Within each tributary stratum, two 21-m seines and one 6.1-m otter trawl 

samples will be collected as outlined in Table 7.2. The sampling sites, sampling dates, 

and order in which reporting units are sampled within a designated sampling period are 

randomly chosen. 

7.2.1 Site Selection 

Sampling site selection will be made following a stratified-random design. In each of the 

rivers, primary sample sites within each stratum will be selected from a universe of points 

that define a line down the middle of the river. An octagonal grid system has been 

overlaid on McKay Bay to create a universe of sampling sites in this system. In 2005, 

sampling began in a portion of Hillsborough Bay that is immediately outside of the Alafia 

River; a universe of sampling sites in this area has been divided into two strata based 

upon distance from the river mouth. In the rivers, a lateral position (left or right) will be 

randomly chosen for each randomly selected sampling site. If the sampling site is to be a 

seine site, the field manager should locate the site and, while facing upriver, turn 90º in

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the direction of the lateral position. The field manager will then travel perpendicular to 

the river’s midline until the shoreline is encountered; this is the site to be sampled. For 

trawl sites, once the randomly selected point is located, the trawl will be pulled into the 

current along the river’s midline. In McKay and Hillsborough Bays, the selected site is 

the site to be sampled. 

If a primary site cannot be sampled (e.g., too deep, submerged obstructions, docks or 

bridges in the way, etc.) an alternate site is to be sampled. A list of alternate sampling 

sites for each sampling period, stratum, and gear is generated by FIM program personnel 

prior to each sampling event. When the primary site cannot be sampled, the field 

manager will work sequentially down the list of alternate sites until a site that can be 

sampled is identified. Sites that cannot be sampled at any time will be noted to better 

define the sampling universe. 

7.2.2 Sampling Protocol 

The HBMP adult and juvenile fish sampling will follow standard FIM protocols (see 

below), with a few additions. Trawls in the rivers and bays will follow procedures 

detailed for river trawls, with five minute (~0.1nm) tows. Seining with the 21-m seine 

will be conducted using boat set seine procedures in the rivers, and offshore seine 

procedures in McKay and Hillsborough bays. Processing of biological samples will 

follow procedures outlined in: 1) Sample Work-up; 2) Subsampling and; 3) 

Representative and Unidentified Sample Collection. Biological data will be recorded on 

the FIM program’s length data sheets. 

All field data will be recorded using the FIM program’s field data sheet following 

standard FIM procedures. Vertical profile Hydrolab casts will be taken at all sampling 

sites. Surface measurement will be taken such that the Hydrolab’s probes are 150 mm 

from the surface. Additional recordings will be taken at 1-m intervals from the surface to 

the bottom. A final Hydrolab reading and depth will be recorded at the bottom. A 

measured rope, attached to the Hydrolab Sonde, will be used to demarcate the depth 

intervals. If the water depth is less than a meter, both surface and bottom readings will 

still be recorded. Hydrolabs must be pre-calibrated the day of sampling and postcalibration 

must occur within 24 hours of completing a day of sampling; all calibrations 

will be in accordance with standard procedures. Station numbers that identify the 

reporting unit and the location within that system will be recorded on the data sheet. 

Additionally, all latitude/longitude coordinates will be recorded to the third decimal 

minute using a GPS. If vertical profiling of temperature and salinity or if location 

information cannot be completed, biological sampling will be terminated. Representative 

and unidentified samples will be brought back to the lab and worked up following 

standard FIM program protocols.

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7.2.3 Data Management 

Data sheets will be filled out by the Principal Investigator (“PI” i.e. the person in charge 

of a sampling trip) in the field and coded upon return to the lab. Additionally, the sheets 

will be independently reviewed by a second crewmember prior to being submitted for 

data entry. All physical and biotic data will be handled following the standard FIM 

program data entry, management, and processing protocols. Once the data have been 

entered, proofed, and completed, corrections based upon the representative and 

unidentified samples will be made to the database. The data will then undergo the FIM 

program error checking and data summary procedures. Documented data will be turned 

over to the project consultant (PBS&J) in Microsoft Access format when the data from 

each quarter has been completely processed and error checked. 

7.3 Standard Fisheries Independent Monitoring Procedures and Protocols 

The following protocols and procedures are designed to define the variables concerning 

the Fisheries-Independent Monitoring elements of the HBMP. Methods and sampling 

protocols used in the fish studies must follow the procedures contained within this 

portion of the QA/QC Plan. These standardized sampling procedures, sampling 

techniques, data recording, etc. will ensure data that can then be combined or compared 

as appropriate. 

Members of the Fisheries-Independent Monitoring elements of the HBMP must read, 

understand and follow all steps in each procedure outlined below. If questions or 

suggestions arise, the Project Manager must be consulted for clarification before any 

further actions can be taken. Under no circumstance should a procedure or steps within a 

procedure be omitted or modified. 

The major protocols and procedures associated with the fisheries elements of the HBMP 

monitoring program are listed below in the order they are presented. 

7.3.1 Overview of Fisheries-Independent Monitoring Program 

7.3.2 Principal Investigator’s Duties 

7.3.3 Stratified-Random Sampling 

7.3.4. Sampling Gears 

7.3.5 Field Sampling 

7.3.6 Laboratory Work-Up 

7.3.7 Data 

7.3.1 Overview of Fisheries-Independent Monitoring Program 

Since the early 1980s, the Fish & Wildlife Research Institute (FWRI) has been 

developing a systematic and continuous fisheries program with the purpose of monitoring 

commercial and recreational marine fisheries and to collect and integrate essential 

information used in the management and enhancement of Florida’s marine resources.

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Two basic components compose Florida’s Marine Fisheries Monitoring Program: the 

Fisheries-Independent Monitoring (FIM) program and the Fisheries-Dependent 

Monitoring (FDM) program. The FDM program is composed of three parts: the Florida 

Marine Fisheries Information System ("trip ticket"), the Trip Interview Program (TIP), 

and the National Marine Fisheries Service’s (NMFS) Marine Recreational Fishery 

Statistics Survey (MRFSS). The Fisheries-Independent Monitoring and Fisheries- 

Dependent Monitoring programs use different strategies to monitor the status of Florida’s 

marine fisheries. The FIM program assesses fish stocks by directly sampling the fish 

population independent of either the commercial or recreational fisheries, whereas the 

FDM program estimates fishing pressure and landings (commercial or recreational) and 

uses those data as indicators of stock structure. 

The design phase of the FIM program began in 1985 with initial funding provided by a 

federal Sport Fish Restoration grant. Between 1985 and 1988, the FIM program’s 

sampling design, gear types, methodologies, and philosophies were developed. 

Preliminary sampling in Tampa Bay was conducted in 1988 and routine monitoring of 

fishery stocks in Tampa Bay and Charlotte Harbor commenced during 1989. Although 

funding under the federal grant continues, the majority of the program is now supported 

by Florida saltwater recreational fishing license fees. 

The FIM program was initially developed to assess the recruitment of young-of-the-year 

resource species into the estuary. This approach allows researchers to be more predictive 

in stock estimates than estimates determined from fisheries-dependent monitoring. Longterm 

fisheries-independent monitoring of juvenile life stages of finfish stocks should 

allow fishery managers to formulate policies proactively rather than reactively, which has 

often been the traditional approach. 

The FIM program’s current objectives are to: 1) establish baseline data on the relative 

abundance of inshore fishes and select macroinvertebrates; 2) detect seasonal and annual 

trends in abundances and determine the underlying environmental factors influencing 

these trends; 3) identify critical habitats utilized by fishes and select macroinvertebrates; 

4) provide data to be used in the determination of critical life history parameters (age and 

growth, maturity, and reproductive cycle) of select species; and 5) provide data for 

developing and assessing the efficacy of fishery management plans. To achieve these 

objectives, the FIM program employs a holistic approach to sampling, using a multispecies, 

multi-gear sampling design to collect information on all species, not just those of 

recreational or commercial importance. The FIM program also records extensive sitespecific 

information on environmental and biological variables that allows researchers to 

evaluate species interactions, habitat dependencies, and the effects of environmental 

influences on fishery recruitment processes. These data, therefore, are not only 

invaluable for traditional stock assessments but can also be used for habitat-suitability 

modeling (HSI), identification of essential fish habitat (EFH), multi-species stock 

assessments, and ecosystem modeling.

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7.3.2 Principal Investigator’s Duties 

This procedure should be used by the Principal Investigator to ensure that all 

responsibilities of the PI are completed. Responsibilities include items that must be done 

before and after a sampling trip. Tasks can be delegated by the PI to other crewmembers, 

as necessary. 

7.3.2.1 Objectives 

1. To ensure that all aspects of a sampling trip are conducted safely. 

2. To ensure that all sampling is completed according to the standard Fisheries- 

Independent Monitoring (FIM) program procedures. 

3. To ensure that each sampling trip is completed to the fullest extent possible. 

4. To ensure that all data sheets, reports and forms are filled out correctly and 

completely. 

7.3.2.2 Pre-Sampling Trip 

1. Obtain sampling dates, collection numbers, sampling type, zone, grid, microgrid, 

spiral directions, and names of crew members from the Bay System Coordinator 

or designee. 

2. Reserve a vehicle and boat for appropriate time period. 

3. Review sampling sites on a gridded chart or topographic map to determine if an 

alternate site is needed. Be aware of areas closed to boats/motors and Submerged 

Aquatic Vegetation policy. 

4. Submit a float plan to the Bay System Coordinator or designee at least three days 

prior to the sampling trip. The float plan should contain grid and microgrid 

information, departure time, and other pertinent information. 

5. Load the boat with all sampling gear needed to complete the sampling trip 

(Appendix 7-A). Check to make sure all needed gear is on board and in good 

working condition. 

6. Procure data books with a sufficient number of data sheets, appropriate safety 

equipment (e.g., first aid box and flares), electronics (e.g., depth meter, GPS and 

cell phone or VHF radio), and Hydrolab. 

7. Check boat and truck for gas and oil. 

8. Notify Marine Patrol of sampling schedule. Before any sampling trip the PI must 

FAX the Marine Patrol and any other relevant agencies using the FWRI field 

activity notification form (Figure 7.2). The Marine Patrol should be faxed the 

morning of the trip.

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9. Obtain personal gear (e.g., foul weather gear and booties). 

10. Obtain and fill cooler(s) with ice. 

11. Go through the trip equipment checklist with the crew before departing. 

12. Complete sampling using standardized FIM program procedures. 

13. It is the responsibility of the PI to be aware of and attempt to fill any Species 

Action Notices. 

7.3.2.3 Post-Sampling Trip 

1. Following every sampling trip, fill the truck and boat gas tanks and check the oil 

levels. 

2. Upon returning to the lab, rinse the boat, trailer and vehicle with freshwater, and 

run the boat engine with fresh water to flush out sand and salt water. 

3. Fish brought back to the lab for identification or as a representative sample should 

be placed in the proper freezer location. 

4. Ensure that all damaged gear has been tagged. 

5. Ensure all gear and electronics have been properly cleaned and stored. 

6. Inform the proper personnel of any problems associated with the boat, electronics 

or gear, and follow up on all repair problems to ensure they have been corrected. 

7. Communicate any problems to the PI in charge of the next sampling trip so 

alternative arrangements can be made if the repairs cannot be finished prior to the 

scheduled sampling. 

8. Make sure all samples brought back to the lab are completely worked up (age and 

growth specimens) or delivered to the appropriate sections (fish health and 

genetic specimens). Make sure all Species Action Notice specimens collected for 

others are stored appropriately and the contact person is notified. 

9. Check the field and length sheets to make sure they are properly filled out. Have 

an experienced crewmember review data sheets against the original selected grids, 

the float plan, and the trip report. This will reduce the number of errors in the 

data set and make analysis much easier. 

10. Submit completed data sheets for data entry within four working days of the trip 

completion. 

11. Submit any public interactions to the FWRI Intranet using the info request 

electronic form. 

12. A trip summary report must be completed for each sampling trip within two 

working days of the trip. Discuss highlights and identify problems that occurred 

during the trip. These should be turned in to the Bay System Coordinator.

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7.3.2.4 Responsibilities 

1. The PI has the responsibility for the safety of the rest of the crew. 

2. The PI is responsible for completing the sampling trip to the fullest extent 

possible. 

3. The PI is responsible for ensuring the collection of representative samples, 

unidentified specimens, and Species Action Notice specimens. 

4. It is the PI’s responsibility to ensure that all equipment has been cleaned and put 

away following completion of a trip. 

5. The PI has the final decision in matters of continuing sampling, aborting samples, 

or adjusting the sampling regime. The PI may also receive input from the crew. 

The PI does not have the authority to modify, omit, or knowingly sample counter 

to procedures. It is the responsibility of the PI to conform to all FIM program 

procedures. 

6. FWRI Field Activity Notification (see Figure 7.2). This is to serve as Notification 

that the following described field activities will be conducted by employees of the 

Florida Fish and Wildlife Conservation Commission/ Fish & Wildlife Research 

Institute. 

7.3.3 Stratified-Random Sampling 

The Fisheries-Independent Monitoring program uses a stratified-random sampling (SRS) 

technique to monitor fish populations in estuarine systems throughout the state of Florida. 

To ensure sampling protocols are the same in all estuarine systems, a set of standardized 

procedures has been developed. To maintain the integrity of the program, it is essential 

that these procedures be adhered 

Stratified-random sampling is conducted throughout the year. Sampling is conducted 

each month in as tight a window as possible to provide a synoptic overview of fish 

abundances throughout the estuaries. For logistical reasons, sampling with the 183-m 

seine and purse net are sometimes combined with the 21.3-m seine and 6.1-m otter trawl 

sampling. All sampling is completed during Period 2 (daytime). 

The selection of sample grids for both juvenile and adult SRS are made by zone, with the 

number of samples taken in each zone proportional to the number of grids that can be 

sampled by that gear baywide. After sample sites have been selected, zone boundaries 

are removed and samples grouped to enhance sampling logistics. The order in which 

these groups of sites are sampled during the sampling period is then randomly selected. 

Sampling sites will be selected and visited as described in Section 7.2.1.

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7.3.3.1 Objectives 

1. To monitor fishes and selected macroinvertebrate populations using stratifiedrandom 

sampling techniques. 

2. To randomly sample various locations within the reporting units of the HBMP. 

3. To ensure that the methods of sampling are standardized. 

4. To utilize the most efficient sampling gear for each specific habitat type. 

7.3.3.2 Procedure 

Principal Investigator’s (PI) Duties 

See Section 7.3.2 

Crew Duties 

1. Assist PI in sampling trip preparation. 

2. Verify that all necessary gear has been loaded prior to departure. 

3. Find out meeting time for departure from the lab. 

4. Collect samples according to established protocols. 

5. Notify PI of any problems encountered. 

6. Upon completion of the trip, ensure that all gear is cleaned and properly stored. 

7. Make sure that all damaged gear has been tagged and that the PI notifies the 

proper personnel of the damage. 

8. Flush the boat motor with fresh water and wash the boat, trailer and vehicle 

thoroughly with fresh water. 

SRS Monitoring 

1. 21.3-m Seines: Following the procedure specified in sections 7.3.4.2 and 7.3.4.3, 

collect samples at randomly selected seine sites. If site selection is by stratum, 

ensure that samples are collected in the pre-determined stratum, or that proper 

alternate site selection criteria are followed. 

2. 6.1-m Otter Trawls: Following Procedure 7.3.4.4, collect samples at the 

randomly selected trawl sites.

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7.3.4 Sampling Gears 

7.3.4.1 21.3-m Center-Bag Seine 

A 21.3-m center bag seine is used to collect juvenile and small adult fish and 

macrocrustaceans along bay edges, river banks, shallow tidal flats and most areas where 

water depth is less than 1.5 m (1.8 m in rivers). Two techniques are employed by the 

FIM program to cover specific habitats. The bay technique is used where the water depth 

is less than 1.5 m, such as tidal flats, mangrove fringes, sea wall habitats, sloping beaches 

and banks. The river technique samples riverine areas and tidal creeks where water depth 

typically increases rapidly (to not more than 1.8 m) from the shoreline, making it 

impossible to use the bay or beach techniques. 

Gear Description 

21.3-m center-bag seine, 1.8-m deep with 3.2-mm #35 knotless nylon Delta mesh. 

Objectives 

1. To produce an accurate, quantitative sample of juvenile and small adult fishes 

which utilize shallow-water habitats. 

2. To estimate relative abundance of small fishes and macrocrustaceans that use 

shallow water regions of the estuary. 

Procedure 

1. Obtain sampling site locations, coordinates, and seining methodology from the 

Bay System Coordinator. 

2. Ensure the starting bag depth is greater than 0.3 m. Note: If at all possible, avoid 

deployment in areas where submerged obstructions (logs, oysters, rocks, etc.) may 

snag the net allowing fish to escape underneath the lead line as the net is 

retrieved. If there are many snags, the sample must be aborted. 

3. Physical data should be taken at the original bag location and recorded following 

the data sheet procedure. 

4. For bay and beach seine techniques, record the percent submerged aquatic 

vegetation (SAV) cover (BOTTOM VEG) as a quantitative estimate over the 

entire area seined. 

5. For river seine technique, record the percent overhanging vegetation cover 

(SHORE TYPE) as a quantitative estimate over the entire shoreline seined. 

Table 7.3 lists the minimum and maximum bag depths and the maximum wing depth for 

the different 21.3-m seine techniques. If the depths do not meet the minimum or 

maximum depth requirements, an alternate sampling site should be selected following 

standard FIM procedures.

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7.3.4.2 Center-bag Seine (21.3-m bay seine technique) 

The 21.3-m bay (previously called offshore) seine technique is currently being used for 

stratified-random sampling in Tampa Bay. 

Objective 

To effectively sample areas within Florida estuaries where the water depth is less than 1.5 

m, such as tidal flats, mangrove fringes, seawall habitats, and shallow sloping beaches 

and banks. 

Procedure 

1. Locate designated site to be sampled. 

2. Attach the 15.5-m (51’) line to the top of the seine poles. Separate the poles until 

the 15.5-m line is taut (Figure 7.3A). 

3. Stretch the seine out perpendicular to the current, with the mouth of the bag 

facing into the current. However, if the wind is strong, and has more influence on 

the seine than the current, stretch the seine out perpendicular to the wind. The 

bag depth (START DEPTH) should be noted at this time. Gear-specific START 

DEPTH guidelines are listed in Table 7.3. 

4. Pull the leads and floats of the bag back until the seine is in a fishing position. 

5. Angle seine poles so that the lead line is slightly ahead of the float line. The 

person walking with the 9.1-m (30’) distance line (and the pivot pole) will walk at 

the same pace as the seiners; walking ahead of the seine may scare fish into or out 

of the net. 

6. Seine into the current or wind. Keep the 15.5-m line taut at all times. 

7. Use the 9.1-m line to measure the distance traveled. 

8. At the end of the 9.1-m line, the two seiners walk directly toward each other as 

rapidly as possible. 

9. Plant a third pole (pivot pole) at the point where the two seiners meet (Figure 

7.3B) and angle the pivot pole to help keep the leads down. 

10. The WING DEPTH should be noted when the two seine poles come together. 

Gear-specific WING DEPTH guidelines for are listed in Table 7.3. 

11. Orient the mouth of the bag into the current/wind to keep the bag open and ensure 

the movement of fish into the bag. 

12. One of the two seiners takes both seine poles and pulls the seine around the pivot 

pole at an angle sufficient to avoid gaps between the pole and the netting (Figure 

7.3C).

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13. The second seiner helps the pivot person hold the pole and guide the seine around 

the pole, keeping the lead line on the bottom at all times by “footing”. The 

second seiner and the pivot person will also push the wings firmly against the 

pivot pole to prevent fish from escaping. In shallow water (

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3. Slowly approach the sampling site, running the boat parallel to shore at the 

shallowest depth possible. Turn the boat away from the shore as the first person 

jumps on or close to shore. 

NOTE: River seines set against an inundated shoreline have a maximum wing depth of 0.5 m 

unless the seine is set along a seawall, where the maximum wing depth is 1.0 m. The minimum 

and maximum bag depth for river seines is 0.3 m and 1.8 m, respectively. If the depths do not 

meet the minimum or maximum depth requirements, an alternate sampling site should be selected 

following standard FIM procedures. 

4. The first person on shore waits while the remainder of the net is deployed. This 

person ensures that there are no escape routes either under the lead line or along 

the shore edge. 

5. The boat operator increases speed and steers a semi-circular course along the 

shoreline, paying out the net until the bag is overboard. 

6. Once the bag is deployed the driver should be notified and the water depth at the 

bag (START DEPTH) should be noted. 

7. The boat operator then turns the boat toward shore, slowing the boat and lifting 

the engine as the boat approaches shore and the end of the seine. 

8. A second person jumps overboard and quickly drags the second pole to shore. 

9. When both seine poles are on shore, they are pulled toward each other, keeping 

the leads on the bottom. 

NOTE: Be sure to get the seine poles as close to the land-water interface as possible. 

10. When the poles are about 1 m apart, drop the poles to begin working the net to 

shore. Note the WING DEPTH at this time. The WING DEPTH is the average 

depth of water, to the nearest 0.1 m, along the path fished by the seine end(s) 

closest to shore. 

11. One crew member begins pulling in the leads while the other two assist by 

holding the wings up and pulling them toward shore. Any fish remaining in the 

wings should be put inside the net. 

12. When the leading edge of the bag’s lead line is on shore, trip the net by lifting at 

the bag/wing junctions. If the sample is muddy, pull the bag offshore and rinse in 

deeper, clearer water. 

13. Reduce the bag size by slowly inverting the bag to concentrate the fish. 

14. Place the sample into a bucket or tub. Large fish may be measured and released 

at this time.

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15. Collect fish that remained in the wings or on the beach and place in the sample. 

Most of the fish should have been put into the bag as it was retrieved. Subsample 

using proper procedures if necessary. 

16. Follow proper sample work-up (Section 7.3.5.1). 

17. Physical data should be taken at the original bag location and recorded following 

the data sheet procedure. 

7.3.4.4 6.1-m Otter Trawl 

A 6.1-m otter trawl is used in the Fisheries-Independent Monitoring (FIM) program to 

sample areas of the estuarine system between 1.8 m and 7.6 m in depth. In addition to 

sampling areas of the bay not accessible to seines, trawls tend to collect epibenthic fish 

and macrocrustaceans that are larger than those typically collected in seines. 


6.1-m otter trawl with 38-mm stretch mesh and 3-mm mesh liner. 

Objective 

To collect an accurate quantitative sample of epibenthic fish found in deeper (1.8 - 7.6 m) 

portions of the bay and rivers. 

Procedure 

1. Preliminary procedures 

A. Obtain sampling site locations and coordinates from the Bay System 

Coordinator. 

B. Arrive at the site safely and without disturbing the area to be sampled. 

C. Record physical data at each site sampled. 

D. Ensure starting depth is less than 7.6 m and greater than 1.8 m. 

NOTE: If water depths shallower than 1.8 m are encountered during a trawl, an 

alternate sampling site should be selected. Exceptions to this rule include sampling 

in rivers and basins (Florida Bay) where an arc tow can be completed in lieu of a 

straight tow (Section 8). Preference, however, should always be given to completing 

a straight-towed trawl when possible. Obtain sampling site locations and coordinates 

from the Bay System Coordinator. 

2. Trawl preparation 

A. Attach the trawl harness to the cleats on each gunwale.

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B. Coil the tow line and bridle forward of the gas tank bulkhead so they 

will deploy without tangling. 

C. Attach the ends of the bridle lines to the trawl doors. 

D. Tie the cod-end of the trawl and ensure a crab float is securely 

attached to the net. 

E. Unfold the net, with the doors separated in the net well, and make sure 

it is not tangled. 

3. Trawl deployment 

A. Position the boat into the current (or wind if no current is detected), 

while keeping forward momentum. 

NOTE: The current within a river can flow backwards (upstream) if a dam is 

holding water back during a rising tide. Open bays may also experience currents that 

flow contrary to predicted tables. Therefore, the current direction should be assessed 

at the sampling site to determine tow direction. 

B. Toss the crab float and cod end over the stern of the boat. 

C. Motor the boat forward at an appropriate speed to deploy the net 

(except the doors) off the stern. 

D. Check the trawl doors to make sure the lead line and float line are not 

crossed. 

E. Swing the trawl doors out and over the stern of the boat. 

F. Record the START DEPTH. If the depth to be sampled is less than 1.8 

m or greater than 7.6 m, choose an alternate site or go to section 8 

below (Special trawl procedures). 

G. Feed doors out at the same speed and watch to make sure they are 

vertical and pulling the net open. 

CAUTION: Stay outside of the bridle and keep feet clear of lines! 

H. Continue to deploy the bridle lines, while keeping equal tension on 

both lines at all times. 

I. When bridle lines are out, the crew should step forward of the bullet 

buoy. One person should maintain tension and continue to guide the 

tow line out. 

J. Deploy the bullet buoy at the end of the tow line. 

K. Tell the boat operator that the net is deployed.

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4. Fishing procedure 

A. Save starting waypoint using a GPS after the tow line has been 

extended and the trawl begins fishing. 

B. Record START TIME: all trawl tows should last 10 minutes, except 

on rivers where a 5 minute tow time is standard. 

C. Set boat speed to tow approximately 0.2 nm in 10 minutes during bay 

sampling or 0.1 nm in 5 minutes during river sampling (1.2 knots and 

~1200-1400 rpm). 

D. Fill out the remaining portion of the field data sheet. 

E. Before the end of the tow, the boat operator should alert the crew so 

that the area near the rear of the boat is clear and ready to receive the 

trawl. A tub with water should be ready to receive the sample. 

5. Trawl retrieval 

A. At the end of the tow, save the present position (ending waypoint), 

using the GPS. 

B. Place the boat in reverse, as needed, while one crew member retrieves 

the tow line as quickly as possible. The tow line and bridle lines 

should be kept taut at all times. Another crew member should coil the 

line forward of the gas tank bulkhead, so the trawl will be ready to be 

deployed. 

C. At the first appearance of the bridle lines, the person coiling the tow 

line should stop coiling and help in retrieving the bridle lines. 

D. Place the boat out of gear or in forward to prevent the boat from 

drifting over the trawl. Record END DEPTH. 

E. Recover the doors and remove any fouling on the net, chain, or doors. 

F. After the doors are in the boat, the wings and the tickler chain should 

be pulled into the boat together to retrieve the net quicker and thus 

help eliminate fish escapement. 

G. Concentrate the sample by shaking it down to the cod end taking care 

not to shake gilled fish out of the net. Remove any remaining gilled 

fish once the trawl is on board. Gilled fish are to be considered part of 

the sample. After removing the catch from the cod end, inspect the 

cod end liner for small fish and invertebrates that may not have been 

removed by shaking down the net. 

H. At some point during trawl retrieval or sample collection the PI should 

determine the DISTANCE TOWED and the BEARING. The distance 

towed is measured from when the tow line becomes taut and the net is 

fishing to when the boat is stopped to begin retrieving the tow line. 

When sampling in the bay, this distance should equal 0.2 nautical

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miles (acceptable range is 0.15 to 0.25 nautical miles) for a 10 minute 

tow. If it does not fall within this guideline, the sample should be 

aborted and speed adjusted accordingly on subsequent trawls. For 

river sampling, see section 8 below. 

NOTE: If the distance towed is not properly taken, the tow has to be repeated. If the 

GPS is inoperative, abort the trip. No trawl tows will be made without a GPS. 

6. Determination of acceptable sample 

A. After several aborts, trawls with a minimum of 60% of the original 

tow time for bay trawls (6 minutes) and river trawls (3 minutes) are 

acceptable. Tow times below these levels should be aborted. 

B. If the bycatch appears to have affected the fishing ability of the trawl, 

the sample must be aborted. Unacceptable bycatch that may affect the 

fishing ability of the trawl include any natural or man-made debris that 

creates a barrier near the mouth, body, or cod end of the net. A large 

volume of bycatch (i.e., exceeding ~50 gallons) may also affect how a 

trawl fishes such that the pressure wave created during towing 

prevents the trawl from fishing properly. 

C. In some cases, a net that gets hung up while towing can indicate the 

point where unacceptable quantities of bycatch have been collected. If 

any of the previously mentioned bycatch types are collected after an 

acceptable period of time (see Step 1) the tow will be discontinued at 

that point and the sample would be accepted, barring any obstructions 

within the net as described in Step 2. In cases where the net gets hung 

momentarily prior to an acceptable tow time, the tow should be 

completed, and a determination of acceptability should be made upon 

retrieval and examination of the net. 

D. Bycatch that includes small portions of crab traps and other manmade 

items that do not appear to affect the trawl opening or otherwise hinder 

normal operation (e.g. twisting of trawl body) would be acceptable. 

E. Trawls will be aborted anytime the gear fails to fish in the manner it is 

intended (i.e. twisted bridle lines, doors flying or not on the bottom, 

mechanical failure). 

F. Samples in which traps or debris are snagged on the outside of the 

trawl body or trawl doors will be aborted if the trawl has become 

twisted, if debris has impeded the flow through the mouth of the trawl, 

if debris is tangled in the bridle, or if debris has changed the shape of 

the opening or body of the trawl.

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7. Sample collection 

A. Empty the sample into the tub and examine the liner to recover any 

fish remaining in the net webbing. 

B. Determine and record bycatch and quantity. 

C. Subsample using proper procedures if appropriate. 

D. Follow proper sample work-up (Section 7.3.5.1). 

8. Special trawl procedures 

A. When trawling in a river, follow normal trawling procedures except 

that the duration of the trawl should be 5 minutes and the distance 

traveled should equal 0.1 nautical miles (acceptable range is 0.08 to 

0.12 nautical miles). 

B. When trawling in water shallower than 1.8 m but greater than 1.0 m, 

execute an arc tow. During an arc tow, the boat should be turned (1/3 

total turning radius) to prevent the trawl from sampling an area 

possibly disturbed by the boat propeller. 

NOTE: If water depths shallower than 1.0 m are encountered during a trawl, the 

trawl should be aborted and an alternate sampling site should be selected. Arc-towed 

trawls should be used as a last resort (i.e., Florida Bay and river sampling). 

9. Trawl storage. When all tows have been completed, the following steps should 

be taken in storing the trawl: 

A. Place the tarp on the deck of the boat and the doors on top of the tarp. 

B. Detach the bridle lines from the doors. 

C. Fold the body of the net onto a door. 

D. Place the chain and crab float over the net. 

E. Place the other trawl door over the net, chain, and float and close the 

tarp. 

F. Wrap tarp over net and secure. 

G. Coil the harness into a gray tub. 

7.3.5 Field Sampling 

7.3.5.1 Sample Work Up 

Introduction 

All fishery samples collected by the Fisheries-Independent Monitoring (FIM) program 

are processed following a standard set of protocols. These protocols ensure that an

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accurate size representation and number collected are recorded for each species in each 

sample and that the data taken reflect the entire catch. All species of fish, select 

macroinvertebrates (Callinectes sapidus, C. similis, C. bocourti, C. ornatus, Menippe 

spp., Limulus polyphemus, Portunus spp., Charybdis hellerii, Platychirograpsus 

spectabilis, Farfantepenaeus aztecus, Farfantepenaeus braziliensis, Farfantepenaeus 

duorarum, Litopenaeus setiferus, Rimapenaeus constrictus, Xiphopenaeus kroyeri, 

Sicyonia brevirostris, S. laevigata, S. parri, Macrobrachium spp., Panulirus argus, 

Stomolophus meleagris, Argopecten spp. and Mercenaria spp.), and turtles are worked up 

for each sample. Additionally, grass shrimp are identified to their lowest practical 

taxonomic level during sampling trips for the HBMP. 

Objective 

To ensure that all fish, selected macroinvertebrates, and turtles collected are counted and 

measured according to a standardized method, the measurements taken are reflective of 

the size structure of the entire sample, and accurate counts of all species are recorded. 

Procedure 

This procedure applies to all stratified-random sampling only for work-up procedure of 

directed sampling specimens. 

1. Cull the sample thoroughly for all fish and selected macroinvertebrates. 

Specimens should be separated by species, selected randomly to be measured 

and counted according to the guidelines listed below. Special care should be 

taken to remove vegetation and other bycatch that may interfere with the workup 

of the sample. Record the type, amount and ratio of bycatch on the field 

data sheet. The 21.3-m seine and 6.1-m otter trawl samples that contain large 

numbers of specimens (>1000) may be split according to procedures 

(Subsampling). 

2. Selected Species 

A. “Selected Species” are species that the FIM program considers to be of 

recreational or commercial importance. These fish should be processed 

first and released alive whenever possible. Not all recreationally and 

commercially important species are included in this list. 

B. If a sample is to be subsampled, a conscious and deliberate effort to 

cull all Selected Species from the sample prior to subsampling must be 

accomplished. 

C. Cull through the entire sample and randomly select up to 40 

individuals for each species designated as Selected Species (100 

individuals prior to 10/97).

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i. If multiple size classes of a particular Selected Species exist, 

then 40 specimens from each size class should be measured. 

More than 40 specimens should be measured when a large size 

range exists with no clear size classes. 

ii. 

If a sample has been subsampled and the species is present in 

both the split and unsplit portions, up to 40 specimens will be 

measured from each size class within both the split and unsplit 

portions. 

D. Count all individuals that were not measured. If different size classes 

were measured, then the number collected within each size class must 

be counted separately. Record numbers on a length data sheet. 

E. Any Selected Species that die should be included in the representative 

sample or brought back for the reference collection. 

3. Common Teleosts 

A. Identify specimens to the species or species-complex level. If unable 

to do so, identify to the lowest taxa possible and return the specimen(s) 

to the lab for further identification. 

i. The following species are only identified to genus level when 

under a certain size: 

a. Eucinostomus spp.

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C. Number of common specimens to measure 

i. 21.3-m seine and 6.1-m otter trawl samples 

a.

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B. Separate Callinectes sapidus and Limulus polyphemus into different 

classifications, i.e., male, female, parasitized (presence of parasitic 

barnacles - C. sapidus only), and unsexed. 

C. For C. sapidus, measure and record 10 (150 

mm CW) specimens of each classification. This measurement should 

be made from tip to tip of the carapace (maximum carapace width; 

Figure 7.4). 

D. For L. polyphemus, measure and record 10 specimens from each 

classification. This measurement should be made at the widest part of 

the prosoma (the front dome-shaped part of the carapace). This is 

generally across the large compound eyes. 

E. Count and record the number of remaining individuals for each species 

and classification (i.e., male, female, parasitized and unsexed). 

6. Penaeid Shrimp 

A. Measure and record post-orbital head length (Figure 7.4) of 10 (40 

prior to 10/97) penaeid shrimp. This measurement should be made 

from the edge of the carapace to the posterior edge of the eye socket. 

B If there is some doubt as to whether the shrimp is a penaeid, treat it as 

an unidentified species. 

7. Cannonball Jellyfish (Stomolophus meleagris) 

A. Measure and record bell diameter of up to 10 cannonball jellyfish in 

21.3-m seines and 6.1-m otter trawls and up to 20 cannonball jellyfish 

in 183-m purse and haul seines. 

B. Count and record the number of remaining individuals. 

8. Grass shrimp 

A. No measurements are taken. 

B. Randomly select ten specimens for identification in the lab. 

C. Count and record the number of remaining individuals. 

7.3.5.2 Subsampling 

Introduction 

Subsampling devices (splitters) or techniques are used to divide samples that contain 

large quantities of organisms or algae into smaller, more manageable subsamples. Data 

from these subsamples can then be multiplied by 2 to the power of the number of splits, 

thus estimating the quantity of the original samples. The time spent removing specimens 

from large quantities of algae or measuring and counting all of the specimens in a large

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sample can affect the success and timely completion of a field trip, making it logistically 

necessary to subsample. Subsampling reduces the time required to work up samples and 

reduces the amount of fish mortality per sample. However, the splitting process must be 

done correctly in order to estimate abundances in the original sample. 

Subsampling can be a source of considerable error in the estimation of original sample 

totals. There are several possible sources contributing to this error: presence of inanimate 

materials limiting the mixing of the sample, presence of rare or unusually large 

specimens, variance of splitting technique, interference (clumping) of organisms, over or 

under-dilution of sample, edge effects, and the repeated subdivision of a sample. Many 

of these factors can be eliminated or controlled by splitter design and standardization of 

the subsampling protocol/technique. 

The following procedures outline the guidelines for subsampling in the field. These 

guidelines are based upon several years of testing the Motoda box splitter by the FIM 

program and should be followed as closely as possible to ensure the validity of 

subsampled data. 

Objective 

1. Establish and maintain a subsampling protocol that will accurately and 

precisely estimate abundance in the original sample while allowing for timely 

sample work-up. 

2. Reduce fish mortality caused by field sampling. 

Procedure 

1. Motoda Box Split 

A. Sample Preparation. This part of the subsampling process is just as 

important as the actual splitting of the sample. It is the responsibility 

of the PI and crew to ensure that the following guidelines are followed. 

The PI and crew must ascertain whether time will be saved by 

subsampling and if the sample can be effectively and accurately 

subsampled. If the sample does not meet these requirements, then 

proceed to work up the entire sample. Samples should contain no less 

than 1000 individuals of a particular species before being considered 

for subsampling. Testing has shown that splitting error increases with 

decreasing sample size. 

i. Remove all vegetation and foreign material from the sample 

(i.e., drift algae, seagrass, sticks, detritus, etc.). 

ii. Cull out specimens: 

a. With a standard length of greater than 100 mm.

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b. That are designated as Selected Species by the FIM 

Program. If there are over 1000 specimens of a 

particular Selected Species, cull 40 and split the rest. 

c. "Rare" species (n

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vii. Pour the split portion of the sample, retained in the receptacle, 

into a net or bucket for work-up or further splitting. If you 

conduct multiple splits on a large sample and split it in portions, 

you should try to maintain the same amount of fish being split. 

This can be accomplished by combining split portions after the 

first or second levels (when the proportions themselves are too 

small to split alone) and splitting them together for the 

remaining split levels. The portions must be at the same split 

level prior to combining. 

viii. Enter the fish from the split portion on a separate length data 

sheet from the unsplit portion. This sheet should have the same 

collection number as the unsplit portion but should indicate the 

SPLIT TYPE (Type 2 for the Motoda Box) and the LEVEL 

(number of times a sample was split). 

ix. Work up sample according to standard procedures. 

2. Table Split Technique 

This splitting technique should only be used in extreme situations (e.g. 

large quantities of drift algae caught in a 21.3-m seine). 

A. Place the entire sample on the sorting table, making sure it is evenly 

distributed and of similar depth throughout. 

B. Measure the long side of the sorting table and divide the sample into 

two equal halves. 

C. Randomly select one half and discard the selected half. 

D. If further subsampling is required, measure the short side of the sorting 

table and divide the sample into two equal halves. 

E. Randomly select one half and discard the selected half. 

F. Work up sample according to standard procedures. 

G. Enter the fish from the split portion on a separate length data sheet 

from the unsplit portion. This sheet should have the same field 

number as the unsplit portion but should indicate the SPLIT TYPE 

(Type 2) and the LEVEL (number of times a sample was split). 

3. Bag Split Technique 

This splitting technique should only be used in extreme situations (e.g. 

large quantities of drift algae caught in the 21.3-m seine) when a table 

split cannot be completed. 

A. After completing the appropriate seining technique, harden the bag 

until all empty space is removed.

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B. Run the 9.1-m seining line from one side, under the bag, and through 

to the other side of the bag, making sure it is in the center, equally 

splitting the sample into two equal halves. 

C. Raise the seine line up until it splits the bag into two equal halves. 

D. Randomly select which half will be removed and discard the selected 

half. 

E. Split again, if necessary, using the same technique. 

F. Work up sample according to standard procedures. 

7.3.5.3 Instructions for Filling Out Data Sheets and Labels 

Introduction 

The success of the Fisheries-Independent Monitoring (FIM) program depends upon 

accurate and complete data being recorded for each sample. All applicable data fields 

must be filled out for each sample collected. 

Objective 

1. To accurately describe the sampling site and conditions at the time a sample is 

taken. 

2. To record taxa, associated lengths, and number of fish and selected 

macroinvertebrates collected in each sample. 

Procedure 

1. The upper left box of the field data sheet and the top line of the length data 

sheet must be filled out for every sample. 

2. Each sample should have its own field and length data sheets. 

Note: If a sample requires additional length data sheets, write ‘1 of 

X’, ‘2 of X’, etc. at the top of the data sheet. 

3. Draw a line through variables to indicate that data were not recorded. 

4. Draw a line through any variable or section of the data sheet that should not 

have data recorded. Any variable left blank will have the previously entered 

data carried over. 

5. If the water depth is less than 1.0 m, take the Hydrolab/YSI data at mid-depth 

and record in the SURFACE row. If water depth is greater than or equal to 1.0 

m, take the Hydrolab/YSI data at both the surface and bottom and record in the 

designated variable. 

6. Ensure that the person recording the data initials all data sheets.

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7. FIELD NUMBER - Because there are so many different sampling projects, care 

must be taken when recording field numbers in the field and lab. This data field 

comprises the “Primary key” that ties all subsequently collected data together. 

The following variables are used, collectively, to create a unique field number. 

All data fields that comprise the field number need to be filled out completely 

on all data sheets and labels. Variable values for each of these data fields can 

be found in Appendix A. 

A. BAY - A two-letter code designating the estuarine system being 

sampled. 

B. TYPE - A one-letter code used to describe the type of sampling. 

C. YEAR - A two-digit numeric code designated to specify the calendar 

year (e.g., 99 = 1999, 00 = 2000). LEADING ZEROS MUST BE 

USED WHERE APPLICABLE. 

D. MONTH – a two-digit numeric code for the month during which 

sampling occurred (01=January, 02=February, etc). LEADING 

ZEROS MUST BE USED WHERE APPLICABLE. 

E. TRIP NUMBER – a two-digit numeric code that uniquely identifies 

each sampling trip within a given month (e.g., 01 is the first sampling 

trip for the month, 99 would be the 99 th sampling trip of the month). 

LEADING ZEROS MUST BE USED WHERE APPLICABLE. 

F. SEQ. NUMBER – a two digit numeric code that uniquely identifies 

each sample collected within a given sampling trip (e.g., 01 is the first 

sample collected, 20 is the 20 th sample collected). LEADING ZEROS 

MUST BE USED WHERE APPLICABLE. 

8. Explanation of data sheets 

A. Field Data Sheets (Figure 7.5) 

• FIELD NUMBER - This must be filled out completely and 

accurately. 

• GEAR - A numeric code that describes the gear and the 

deployment method. 

• REP - A numeric value describing the replicate sample number 

within a grid or fixed station site. Replicate sampling was 

discontinued 04/96. Rep should now always be recorded as 1, 

except for the replicate hydrolab vertical profile collected during 

the HBMP. 

• DAY - Day of the month (USE LEADING ZEROS)

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• STATION - A numeric code that identifies the fixed station 

sampled (see Appendix C in archive manual), the sampling areas 

for the HBMP study, and the sampling areas for directed mullet 

sampling. 

• START TIME - Recorded on a 24-hour time clock in hours and 

minutes (HH:MM). Record at the beginning of a set. USE 

LEADING ZEROS TO FILL ALL BOXES. 

• START DEPTH - A numeric value designating the water depth at 

the beginning of sampling. Recorded to the nearest 0.1 meter. 

• For 21.3-m the water depth should be taken at the 

bag. 

• For trawls, the water depth should be taken as the 

trawl is first deployed. 

• STRATA - A one-character, alphabetic variable to describe the 

habitat sampled. 

• NAV INST - The type of navigation equipment used to determine 

sampling location. Recorded in the box to the left of "LOCATION 

DATA". 

• LATITUDE / LONGITUDE - Read these numeric values from the 

GPS upon arrival at a sampling site. 

• ZONE - A letter code designating the major subdivisions of an 

estuarine system (refer to labeled charts). 

• GRID - A numeric value designating one of the 1x1 nautical mile 

subdivisions in a zone (refer to labeled charts). 

• MICROGRID - A numeric value designating one of the 100 

subdivisions in a grid (refer to microgrid templates). A discrete 

microgrid is to be recorded for each sample. 

• PI/CREW - Record the initials or the name of each crewmember. 

• BOAT - A letter designating the specific boat used. If no boat is 

used, draw a line through field. 

• NOTES - Any additional information the PI wishes to note, such as 

unusual occurrences, habitat interpretations, grid rotations, etc.

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• PROJECTS - A four-character variable that identifies the projects 

to which a collected sample can be applied. 

• INST - Instrument used to measure water quality. Record 

separately for each water quality measurement taken. 

• DO - Read from Hydrolab, record in mg/l to 1/10th increments. 

• pH - Read from Hydrolab, record to 1/10th increments. 

• TEMP - Read from Hydrolab, record in °C to 1/10th increments. 

• SALIN - Read from Hydrolab, record in ppt to 1/10th increments. 

• COND - Read from Hydrolab, record in umhos/cm to 1/10th 

increments. 

• TURB - Read from Hydrolab, record to 1/10th increments. 

• SECCHI - Depth at which secchi disc was still visible on the nonshaded 

side of the boat. Recorded to the nearest 0.1 of a meter. 

• BOTTOM VISIBLE - A yes (Y) or no (N) variable to indicate 

whether the secchi disk was on the bottom when the reading was 

taken. 

• START WIND - An alphanumeric code designating the direction 

the wind was coming from and speed in miles per hour. Record at 

the beginning of a set. E.g., wind coming from the northwest at 5 

m.p.h. = NW 05. If there was no wind, the direction is 0 and the 

speed is 0. 

• START CLOUDS - A numeric value designating the percentage of 

cloud cover, rain, or fog. Record at the beginning of a set. 

• START TIDE - A letter code designating the tidal stage. Record at 

the beginning of a set. 

• PERIOD - A numeric value designating the period of a 24 hour 

day. 

• BOTTOM - Single-letter codes designating the most abundant 

bottom types (composition of the bottom being sampled). Up to 

four bottom types, in decreasing order of percent composition may 

be recorded.

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• BOTTOM VEG - Single-character codes designating the most 

abundant types of attached submerged aquatic vegetation (SAV). 

Up to four bottom vegetation types, in decreasing order of percent 

composition may be recorded. 

• RATIO - The ratio of primary bottom veg, shore type or bycatch to 

secondary bottom veg, shore type or bycatch. For example, if a 

shore type consists of 60% red mangroves and 40% Brazilian 

peppers, the ratio would be 6:4. If there is only one shore type, 

and it covers 70% of the shoreline sampled, the ratio would be 7:0. 

• BOTTOM VEG % COVER - A percentage (0 - 100%) of the 

sampled area that is covered by SAV: 0 if no SAV sampled to 100 

if area is completely covered. 

• BANK - A one-character code designating the general slope of the 

bank and the inundation of the shore type. 

• SHORE TYPE - Two-character codes that designate the prominent 

features on the shoreline. Up to four shore types, listed as one 

moves from water onto land, can be recorded at each sampling site. 

• BYCATCH - Two-character codes that designate the composition 

of the bycatch in a sample, including noncommercial invertebrate 

fauna, algae, grass, etc. Up to four bycatch types, in decreasing 

order of percent composition may be recorded. 

• QUANTITY - Estimate of the amount of bycatch in tenths-ofgallon 

increments. Quantity = 0.0 if there is no bycatch. 

• CURRENT REL - A numeric value recorded in degrees 

designating the degrees from facing current. 

• WIND REL - A numeric value recorded in degrees designating 

orientation with respect to the wind. 

• WING DEPTH - Depth of water (nearest 0.1 m) at the point where 

the bag will be retrieved. Used for all seines. Gear-specific WING 

DEPTH guidelines are listed in Table 7.3. 

• NET WIDTH - Currently recorded only for the purse seine; 

describes the circumference of the set. Used to calculate the area 

of the set.

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• DISTANCE TO BAG - Currently recorded only for the purse 

seine. This variable is used to describe how twisted the net is 

around the purse line once the net has been pursed. 

• SHORE DISTANCE - Distance from the water-land interface to 

the net wing closest to shore, recorded to the nearest whole meter. 

• BANK DISTANCE - Distance from the mean high water mark to 

the net wing closest to shore, recorded to the nearest whole meter. 

• SOAKTIME - A numeric value, recorded in hours and minutes 

(HH:MM), equal to the amount of time a gear was fishing. 

Currently recorded for trawls only. USE LEADING ZEROS TO 

FILL ALL BOXES. 

• END DEPTH - A numeric value recorded to the nearest 0.1 meter 

designating the water depth at the end of a set. Currently recorded 

for trawls only. 

• DISTANCE TOWED - A numeric value calculated using the GPS 

(nautical miles). Record to the hundredth place. This must be 

recorded for each trawl tow or the tow must be repeated. Recorded 

for trawls only. 

• BEARING - A numeric code designating the direction (in degrees) 

towed during trawling; supplied by GPS when calculating distance 

towed. Recorded for trawls only. 

• SEARCH TIME - The time between the start of a search and the 

set of a net. Used during directed sampling only. 

• END TIME - Recorded on a 24-hour time clock in hours and 

minutes (HH:MM). Currently recorded during directed sampling 

only. Record as the time the net is set or when searching is 

terminated. USE LEADING ZEROS TO FILL ALL BOXES. 

• NUMBER OF PANELS -The total number of panels that were 

deployed during a set, rounded to the nearest half panel. Used 

during directed mullet sampling only. 

• GEAR DESCRIPTION - Record the gear type, number, and any 

damages for gears used during a sampling trip. 

• RECORDED - The initials of the person who took the data.

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• REVIEWED - The initials of the person who reviewed the data 

prior to entry. 

• ENTERED - The initials of the person who entered the data. 

• PROOFED - The initials of the people who proofed the data. 

• COMPLETE - The initials of the person who made the final 

corrections. 

Note: It is the PI’s responsibility to ensure that all data are recorded 

correctly and that all appropriate data fields are filled out in the field. 

B. Length Data Sheets (Figure 7.6) 


accurately and must exactly match the field data sheet at each 

sampling site. 

• DAY - Day of the month (USE LEADING ZEROS) 

• REP - A numeric value describing the replicate sample number 

within a grid or fixed station site. Replicate sampling was 

discontinued 4/96. Rep should now always be recorded as 1. 

• STATION NUMBER - A numeric code that identifies the station 

sampled (see Appendix C in archive manual), the sampling areas 

for the HBMP study, and the sampling areas for directed mullet 

sampling. 

• SPLIT TYPE - A numeric description of the type of splitter device 

used for sub-sampling (e.g., 2 is a two-way splitter). 

• LEVEL - A numeric value defining the total number of times a 

sample was split. If a sample is put through the splitter twice, then 

the level would be 2. 

• GENUS/SPECIES - Abbreviate the genus with the first letter 

(capital) and spell out completely the species name (lower case), 

i.e., P. cromis. For unidentified fish follow specified procedures. 

• LENGTHS - Numeric values reflecting the size in millimeters for a 

particular species/taxon. See procedures for specific measurement 

guidelines. 

• N - A numeric value representing the total number of animals 

counted for a particular species, in that specific record.

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Note: When splitting a sample, the actual number of fish counted 

should be recorded. Do not multiply values by split level; a computer 

program has been written to make these adjustments. 

• SEX - Record for all specimens collected in which a positive sex 

identification can be made (e.g., elasmobranchs, Callinectes spp., 

etc.). Do not use drumming to determine sex. 

• USE - A letter code designating the use of specimens. 

• SC - (Size Class) When more than one size class is collected in a 

single sample (e.g., 10-30 mm and 40-70 mm F. majalis) a value of 

A is given for the first size class and B for the second (additional 

letters can be used to denote additional size classes as necessary). 

If only one size class is collected, then no size class designator is 

used. 

• FHC - (Fish Health Code) Designates injured or sick fish that were 

brought back to the lab for fish health evaluation (see Appendix 7- 

A for code list). 

• TALLY LINE - The blank line below the 20 standard length boxes 

is used for keeping a tally of the number of animals counted for a 

particular species/taxon. 

• RECORDED - The initials of the person who took the data. 

• REVIEWED - The initials of the person who reviewed the data 

prior to entry. 

• ENTERED - The initials of the person who entered the data. 

• PROOFED - The initials of the people who proofed the data. 

• COMPLETE - The initials of the person who made the final 

corrections. 

• REP SAMPLE CHECKED - The date and initials of the person 

who checked / changed the representative samples and length data 

diskette. 

• ID CHECKED - The date and initials of the person who checked / 

changed the identification samples and length data diskette.

Page 33 

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C. Sample Labels (Figure 7.7) 


accurately and must exactly match the field data sheet at each 

sampling site. 

• DATE - Complete date including month, day, and year. 

• GRID - The grid number of the sample area. 

• GEAR - Either the gear code or a short description of the sampling 

gear. 

• REP / ID - Circle REP if the specimen is being brought back as a 

representative sample. Circle ID if the specimen is being brought 

back as an identification sample and write down what the specimen 

was called in the field. 

• CULLED FOR - If the specimen is not being brought back for an 

identification or rep sample, write the reason why the specimen is 

being brought back. 

• TO BE CONFIRMED? - Used if the specimen needs to be 

identified a second time. 

9. Data Tracking 

A. All data sheets should be turned in to the Bay System Coordinator 

within four working days following the completion of a sampling 

event. This provides adequate time for coding and peer review of the 

sheets. If this is not sufficient time, notify the Bay System 

Coordinator and provide some estimated time for completion. It is 

very important that the data sheets be turned in quickly and correctly, 

making sure that the data tracking sheets are completed. All data will 

be processed (entered, proofed, corrected, re-proofed, and recorrected) 

at the lab that collected the data. 

B. Completed data should be turned over to the FIM program’s database 

manager quarterly in April (January - March data), July (April - June 

data), October (July - September data) and January (October - 

December data). Emergency use of the data may necessitate the 

acceleration of this timetable at certain times for certain labs.

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7.3.5.4 Representative and Unidentified Sample Collection 

Introduction 

Accurate field identification is essential to the success of the Fisheries-Independent 

Monitoring program. Specimens that represent the catch from a specific area 

(Representative Samples) are brought back to the lab to verify the accuracy of field 

identifications. Specimens that cannot be identified to the lowest possible taxonomic 

level in the field are returned as Unidentified Samples. The procedures for bringing back 

samples are described below and should be followed under all circumstances. It is the 

PI’s responsibility to ensure that adequate representative samples and unidentified 

samples are brought back as often as needed. 

Objective 

1. To confirm the accuracy of field identification. 

2. To correctly identify specimens that are difficult to identify in the field. 

3. To correctly identify specimens which are unidentifiable in the field. 

Procedure 

1. REPRESENTATIVE SAMPLES: The representative sample should be an 

accurate representation of the actual catch of each day in each habitat or area. A 

representative sample of each species should be brought back the first time each 

species is collected during a sampling trip within a general habitat or area. 

A. Complete a representative sample label and place it in a plastic sample 

bag. 

B. Measure specimens to be retained for the representative sample and 

place specimens in the sample bag. 

i. Representative samples should only include specimens identified 

to their lowest possible taxonomic level (e.g., Anchoa mitchilli 

or Menidia spp.). The identification should represent the lowest 

classification the field crew is comfortable making. Specimens 

that can be classified to a lower taxonomic level (e.g., Mugil 

spp.) in the lab should NOT be placed in the representative 

sample. These specimens should be brought back for 

identification and are handled as indicated in the "Unidentified 

Samples" section of this procedure. 

C. Record the lengths of the specific specimens placed in the specimen 

bag on a separate line from the rest of that species and place an ‘S’ in 

the USE box on the length data sheet. This makes it easier for the 

person correcting the database to locate the sample.

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i. Representative samples represent all specimens for that area/day. 

Any mis-identifications that are made will be reflected 

throughout that entire day’s sampling. 

D. A minimum of 3 specimens from each species should be retained for 

the representative sample when they are first collected in a particular 

day, gear, and area. If only 1 specimen of a particular species is 

present when it is first collected, retain 2 more specimens from 

subsequent samples for the representative sample. It is always better 

to keep more than less, and to keep problematic specimens as ID 

samples. 

i. No samples are taken from large, healthy specimens of 

distinctive species that can be unequivocally identified in the 

field. The following list shows size limits of species that do not 

need to be brought back. 

a. Carapace length >30 mm 

(1) Callinectes sapidus 

(2) Menippe mercenaria 

b. Standard Length >75 mm 

(1) All Selected Species 

(2) All Elasmobranchs 

(3) Lactophrys quadricornis 

(4) Chilomycterus schoepfi 

(5) Bagre marinus 

(6) Arius felis 

(7) Selene vomer 

(8) Lagodon rhomboides 

(9) Chaetodipterus faber 

E. Put representative samples on ice. 

F. After returning to the lab, place the representative sample in its correct 

location in the freezer for work-up, following procedures outlined in 

Section 7.3.6. 

2. UNIDENTIFIED SAMPLES: The unidentified sample (ID sample) represents 

specimens that are unidentifiable to their lowest taxonomic level in the field. 

These samples should include both specimens that can’t be identified to any 

taxonomic level in the field and specimens that can be classified to a lower 

taxonomic level in the lab. 

A. Complete an ID sample label and place it in a plastic sample bag.

Page 36 

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B. Identify specimens to their lowest level that the field crew is 

comfortable making. ID samples should only include specimens that 

have not been identified to their lowest possible taxonomic level. 

C. Record the lengths of the specific ID specimens placed in the sample 

bag on a separate line for each species and record an “I” in the “USE” 

box on the length data sheet. This makes it easier for the person 

correcting the database to locate the sample. 

i. Only those fish actually returned to the lab will be adjusted in 

the database. If the sample you are returning to the lab 

represents only part of the unidentified specimens, it should be 

indicated on the data sheet ("Same as ID") that these specimens 

represent all similar specimens captured in that sample. 

a. For species that are problematic or very small, a 

minimum of 10 individuals should be retained. 

b. If there is any indication that more than one species has 

been collected (e.g., A. mitchilli and A. hepsetus), at least 

20 individuals should be included in the ID sample. 

c. If more than one size class of a problematic species 

(e.g., M. cephalus and M. curema) exists, up to 20 

individuals should be retained from each size class and 

indicated by an “A” and “B” recorded in the “SIZE 

CLASS” box. 

ii. If similar specimens are captured in subsequent samples, another 

ID sample should be taken or it must be indicated on the data 

sheet. The label should show that these specimens are similar to 

those brought back earlier in the ID sample ("Same as ID from 

field number ####"). 

D. Place ID sample in bag and put sample on ice. 

E. After returning to the lab place the ID sample in the correct location in 

the freezer for work-up, following procedures outlined in Section 

7.3.6. 

7.3.5.5 Hydrolab Operation and Calibration 

Introduction 

The Hydrolab units must be calibrated routinely to assure the accuracy of their field 

readings. At a minimum, the units must be calibrated monthly. If during calibration, the 

readings in known solutions are outside the desired range of accuracy, the calibration 

interval should be reduced. The parameters that are measured include temperature,

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salinity, conductivity, dissolved oxygen, and pH. The accurate recording of these data is 

important because it can indicate the water quality at the sampling site and may reflect 

trends in water conditions. 

For the HBMP, each hydrolab unit is calibrated in the morning on which the sampling 

trip is to be conducted and is post calibrated within 24hrs of the completion of the 

sampling trip. Calibration instructions for specific Hydrolab models are listed below. 

These instructions may differ slightly from those outlined in Section 5 which describes 

calibration methodologies for YSI models. When utilizing one of the specified Hydrolab 

models listed below, follow the specific calibration methods for that model. 

Objective 

1. To accurately record hydrologic data at sampling sites. 

2. To calibrate Hydrolab units properly to ensure accurate readings during field 

sampling. 

Procedure 

1. Operation 

A. Before leaving the lab, check the voltage on the display of the 

Hydrolab unit. A fully charged unit should have ~14 volts. However, 

as a Hydrolab gets older, a fully charged battery may not reach 14 

volts. Take each unit on an individual basis and know what each unit 

will hold and when it will stop working. Always bring a thermometer 

and refractometer on sampling trips. The Hydrolab units can and do 

become inoperable. At a minimum, salinity and temperature should be 

taken at all sampling sites or the sampling should not be done. 

B. Keep the probes immersed in water at all times. Exposure to air will 

dry out the membranes, which, in turn, will give false readings. 

C. Once on station, turn the unit on and allow it to equilibrate for at least 

5 minutes before taking the readings. 

D. The Hydrolab readings should be taken at the farthest point from shore 

(i.e., at the seine bag or at the outside pole for shoreline seines). 

E. Take vertical profiles as outlined in Section 7.2.2. 

F. Wipe down the unit upon returning to the lab. Rinse the probes with 

clean, fresh water and replace the water in the Hydrolab storage cup 

with clean tap water. 

G. Leave the Hydrolab carrying case open to allow the unit to air dry. 

2. Surveyor II Calibration

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NOTE: Do not turn off the display unit during calibration. You will 

lose any changes that you have made. 

A. Calibration: Conductivity. The parameters are salinity compensated. 

Therefore, conductivity must be calibrated first. 

i. When calibrating for use in seawater a 0.5 Molar KCL solution 

(58.640 millimoles/cm) is prepared by adding 37.28 grams of 

KCL (Fisher Scientific P217- 500, Potassium Chloride) to 1000 

ml of de-ionized (DI) water. 

ii. 

iii. 

Fill the calibration cup with conductivity solution until the 

conductivity probe is covered completely. Cover the calibration 

cup with the soft cap and allow 1-2 minutes for temperature 

equilibrium to be reached. 

Record initial conductivity reading on the calibration sheet (1bbefore; 

Figure 7.8). 

iv. Use the SLOPE function to calibrate conductivity value to 

standard used and record value (1b-after; Figure 7.8). 

v. Rinse twice with DI water 

B. Calibration: pH 

i. Remove the calibration cup and gently pull the pH probe off 

while holding sonde unit with probes down. 

ii. 

iii. 

iv. 

Empty the probe of reference solution and replace with fresh 

standard (Reference Electrode Filling Solution, 3 Molar KCL 

solution buffered to pH 7. This solution can be made by adding 

55.91 grams of KCL (Fisher Scientific P217- 500, Potassium 

Chloride) and 1/2 of a packet of yellow pH 7 buffer powder 

(Hydrion Buffer Chemvelope) to 250-ml of DI water. 

While holding the sonde with probes down, reattach the probe. 

Flip the sonde unit so probes are up and screw the calibration 

cup back on the sonde unit. Rinse with pH 7 solution. 

v. Fill the calibration cup with pH 7 solution (above D.O. 

membrane) and cover with soft cap. (Allow 1-2 minutes for 

temperature equilibrium). 

vi. 

Switch to pH and record initial value on calibration sheet (2c-pH 

before; Figure 7.8). 

vii. Calibrate to 7.00 using ZERO function and record value (2c-pH 

after; Figure 7.8). 

viii. Rinse twice with DI water. 

ix. Rinse the probe with pH 10 solution.

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x. Fill the calibration cup with pH 10 solution (above D.O. 

membrane) and cover with soft cap. Allow 1-2 minutes for 

temperature equilibrium. 

xi. 

Record initial pH value on calibration sheet (2f-pH before; 

Figure 7.8). 

xii. Calibrate to 10.00 using SLOPE function and record value (2fpH 


xiii. Rinse twice with DI water 

xiv. Recheck pH 7 value and recalibrate, if necessary (2h-pH before / 

pH after; Figure 7.8). 

C. Calibration: Dissolved Oxygen (D.O.) 

i. Check the dissolved oxygen membrane. Replace D.O. 

electrolyte and membrane, if necessary. 

NOTE: If the D.O. membrane was changed, the unit must sit 

overnight before calibration. 

ii. 

iii. 

iv. 

Fill the calibration cup with DI water so the tip of the D.O. 

sensor is ~5 mm above liquid surface. 

Blot away any moisture on the membrane surface. 

Place the hard cap on the calibration cup, switch to temperature, 

and let equilibrate. Allow five minutes for water saturation and 

temperature to equilibrate. At this time, temperature should 

change less than one tenth of a degree in five seconds. 

v. Record temperature on the Hydrolab Calibration Sheet (Figure 

7.8). 

vi. 

Record barometric pressure (760 mm Hg at sea level) on the 

Hydrolab Calibration Sheet (Figure 7.8). 

vii. Refer to Table 7.4 for D.O. value corresponding with the 

temperature at 760 mm Hg barometric pressure and record (3- 

Table D.O.; Figure 7.8). 

viii. Switch to D.O., let equilibrate, and record value (3-D.O. before; 

Figure 7.8). 

ix. Use the Slope function to calibrate D.O. to Table value and 

record new value (3-D.O. after; Figure 7.8). 

D. Calibration: Depth (if installed) 

i. Empty calibration cup and leave open (no cap needed). 

ii. 

Switch to depth and record initial value (4-Depth before; Figure 

7.8).

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Section 7.0 

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iii. Calibrate to 000.0 using ZERO function and record new value 

(4-Depth after; Figure 7.8). 

E. Battery and Save 

i. Record value (volts) and save calibration by pulling both the 

ZERO and SLOPE toggles toward you simultaneously (the word 

SAVE will appear on the display). 

ii. Charge the battery, if necessary. 

3. Surveyor III Calibration 

NOTE: The calibration of a Surveyor III is automatically saved after 

each calibration step. 



i. When calibrating for use in seawater, a 0.5 Molar KCl solution 


KCl (Fisher Scientific P217-500, Potassium Chloride) to 1000 

ml of de-ionized (DI) water. 

ii. Fill the calibration cup with conductivity solution until D.O. 

membrane is covered completely. Cover calibration cup with 

the soft cap and allow 1-2 minutes for temperature equilibrium. 

iii. Record initial conductivity reading on the Hydrolab Calibration 

Sheet (1b-before; Figure 7.8). 

iv. Press CALIBRATE and SpC/S keys. Select C for specific 

conductance and press ENTER. 

v. Type in the value of the conductivity standard used (58.64 

mmho/cm), then press ENTER. The conductivity is calibrated 

and the unit returns to standard operating mode (SOM). Record 

calibrated conductivity value (1b-after; Figure 7.8). 

vi. Rinse the probe twice with DI water. 


i. Remove the calibration cup and gently pull pH probe off while 

holding the sonde unit with probes facing down. 

ii. Empty the probe of reference solution and replace with fresh 

standard (Reference Electrode Filling Solution, Fisher Scientific 

SP135-500, 4 Molar KCl saturated with Silver Chloride). 

iii. While holding the sonde with probes facing down, reattach the 

probe.

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iv. Flip the sonde unit so probes are facing up and screw the 

calibration cup back on the sonde unit. Rinse the probe twice 

with pH 7 solution. 




vi. Monitor the display in SOM and record initial value (2c-pH 


vii. Press CALIBRATE and pH keys. 

viii. Type in 7.00 for the value of the pH, then press ENTER. The 

unit returns to SOM. Record pH value (2c-pH after; Figure 7.8). 

ix. Rinse the probe twice with DI water. 

x. Rinse the probe twice with pH 10 solution. 

xi. Fill the calibration cup with pH 10 solution (above the D.O. 

membrane) and cover with the soft cap. Allow 1-2 minutes for 


xii. Monitor the display in SOM and record initial value (2f-pH 


xiii. Press CALIBRATE and pH keys. 

xiv. Type in 10.00 for the value of the pH, then press ENTER. The 

pH system is calibrated and the unit returns to SOM. Record pH 

value (2f-pH after; Figure 7.8). 

xv. Rinse twice with DI water. 

xvi. Recheck pH 7 value and recalibrate, if necessary (2h-pH before / 


C. Calibration: Dissolved Oxygen (D.O.) 

i. Check the dissolved oxygen membrane. Replace D.O. 

electrolyte and membrane, if necessary. If the D.O. membrane 

was changed, the unit must sit overnight before calibration. 

ii. Fill the calibration cup with DI water so the tip of the D.O. 

sensor is ~5 mm above liquid surface. 

iii. Blot away any moisture on the membrane surface. 

iv. Place the hard cap on the calibration cup. Allow five minutes 

for water saturation and temperature to equilibrate. At this time, 

temperature should change less than one tenth of a degree in five 

seconds.

Page 42 

Section 7.0 

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v. Record temperature on the Hydrolab Calibration Sheet (3c- 

Temp; Figure 7.8). 

vi. 

Record barometric pressure on the Hydrolab Calibration Sheet 

(3c-Barometric Pressure; Figure 7.8). 

vii. Check Table 7.4 for D.O. value corresponding with the 

temperature at 760 mm Hg barometric pressure and record (3c- 

Table D.O. ; Figure 7.8). 

viii. Monitor the D.O. in standard operating mode (SOM), let 

equilibrate, and record value (3c-D.O. before; Figure 7.8). 

ix. Press CALIBRATE and %S/DO keys. Select O for D(O) and 

press ENTER. 

x. Enter the local barometric pressure in mm of mercury (760 mm 

Hg at sea level), then press ENTER. 

xi. Type in the D.O. value from Table 7.4 then press ENTER. The 

D.O. is calibrated and the unit returns to SOM. Record 

calibrated D.O. value (3-D.O. after; Figure 7.8). 


i. Empty the calibration cup and leave open (no cap needed). 

Record initial depth (4-Depth before; Figure 7.8). 

ii. Press CALIBRATE and DEPTH keys. 

iii. Type in 0.0 and press ENTER. 

iv. The depth is calibrated and the unit returns to SOM. Record 

new depth value (4-Depth after; Figure 7.8). 

E. 5) Battery 

i. In SOM, press SCREEN to display screen 2 and record the 

internal battery voltage. If there is an external battery, check its 

voltage. 

ii. Charge the batteries, if necessary. 

4. Surveyor IV Calibration 

A. When using a Minisonde attached to the Surveyor IV, the circulator 

must be turned off before calibration. 

i. To turn the stirrer off, press SETUP/CAL. Then press SETUP. 

ii. Press SONDE, arrow down to Circltr: OFF/ON, and press 

SELECT.

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Section 7.0 

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iii. Arrow over to the correct number and press SELECT, then press 

DONE. 

iv. Repeat steps i-iii to turn stirrer back on. 

B. Calibration: Conductivity. The parameters are salinity compensated. 


i. When calibrating for use in seawater, a 0.5 Molar KCl solution 


KCl (Fisher Scientific P-217, Potassium Chloride) to 1000 ml of 

de-ionized (DI) water. 


membrane is covered completely. Cover calibration cup with 

the soft cap and allow 1-2 minutes for temperature equilibrium. 


Sheet (1b-Before; Figure 7.8). 

iv. Press the SETUP/CAL key. Press CALIBRATE and SONDE. 

Arrow down to SpCond:mS/cm and press SELECT. 


mmho/cm), then press SELECT. The conductivity is calibrated 

and the unit returns to the main screen. Record the new 

conductivity value (1b-After; Figure 7.8). 

vi. Rinse the probe twice with DI water. 

C. Calibration: pH 

i. Remove the calibration cup. 

a. With an H20 or Reporter Sonde, gently pull the pH probe 

off while holding the sonde unit with probes facing 

down. 

b. With a Minisonde, unscrew the reference electrode 

housing screw. 



S35-500, 3 Molar KCl saturated with Silver Chloride). Reattach 

the probe cover. 

a. With an H20 or Reported Sonde, hold the sonde unit with 

probes facing down and reattach the probe cover until 

flush with sonde. 

b. With a Minisonde, reattach the reference electrode 

housing screw until flush with sonde.

Page 44 

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iii. 

Position the sonde unit so the probes are facing up and screw the 

calibration cup back on the sonde unit. Rinse the probe twice 

with pH 7 solution. 

iv. Fill the calibration cup with pH 7 solution (above D.O. 



v. Record initial pH value (2c-pH before; Figure 7.8). 

vi. 

Press the SETUP/CAL key. Press CALIBRATE and SONDE 

keys. Arrow down to pH:Units and press SELECT. 

vii. Type in 7.00 for the value of the pH, then press SELECT. The 

pH is calibrated and the unit returns to the main menu. Record 

new pH value (2c-pH after; Figure 7.8). 

viii. Rinse the probe twice with DI water. 

ix. Rinse the probe twice with pH 10 solution. 

x. Fill the calibration cup with pH 10 solution (above the D.O. 



xi. 

Monitor the display on the main screen and record the initial 

value (2f-pH before; Figure 7.8). 

xii. Press the SETUP/CAL key. Then press CALIBRATE and 

SONDE keys. Arrow down to pH:Units and press SELECT. 

xiii. Type 10.00 for the value of the pH, then press SELECT. The 

pH system is calibrated and the unit returns to the main screen. 

Record new pH value (2f-pH after; Figure7.8). 

xiv. Rinse the probe twice with DI water. 

xv. Recheck pH 7 value and recalibrate, if necessary (2h-pH before / 


D. Calibration: Dissolved Oxygen (D.O.) 

i. Before beginning the calibration process, check the D.O. 

membrane. Replace D.O. electrolyte and membrane, if 

necessary. If the D.O. membrane was changed, the unit must sit 


ii. 

iii. 

iv. 

Rinse the probe twice with DI water, then fill the calibration cup 

with DI water so the tip of the D.O. sensor is ~5 mm above the 

liquid surface. 

Blot away any moisture on the membrane surface. 

Place the hard cap on the calibration cup. Allow five minutes 

for water saturation and temperature to equilibrate. At this time,

Page 45 

Section 7.0 

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seconds. 

v. Record temperature and barometric pressure (760 mm Hg at sea 

level) on the Hydrolab Calibration Sheet (Figure 7.8). 

vi. Check Table 7.4 for D.O. value corresponding with the 

temperature at 760 mm Hg barometric pressure and record value 

(3c-Table D.O. ; Figure 7.8). 

vii. Let the dissolved oxygen value equilibrate, and record value (3c- 

D.O. before; Figure 7.8). 

viii. Press the SETUP/CAL key. Then press the CALIBRATE and 

SONDE keys. Arrow down to DO:mg/l and press SELECT. 

ix. Type in the D.O. value from Table 7.4 and press SELECT. 

Record the new D.O. value on the Hydrolab Calibration Sheet 

(3c-D.O. after; Figure 7.8). 

E. Turn the circulator back on following steps Ai-iii. 

F. Battery 

i. Record the voltage on the Hydrolab Calibration Sheet (Figure 

7.8) and recharge, if necessary. 

5. Scout II Calibration 

NOTE: The calibration of a Scout II is automatically saved after each 

calibration step. 



i. For seawater, a 0.5 Molar KCl solution (58.640 millimoles/cm) 

is prepared by adding 37.28 grams of KCl (Fisher Scientific P- 

217, Potassium Chloride) to 1000 ml of de-ionized (DI) water. 


membrane is covered completely. Cover the calibration cap 

with the soft cup and allow 1-2 minutes for temperature 

equilibrium. 


Sheet (1b-before; Figure 7.8). 

iv. Press CALIBRATE, select C:Cond, and press ENTER. 


mmho/cm), then press ENTER. 

vi. Select Y to save calibration. The conductivity is calibrated and 

the unit returns to the main screen. Record new conductivity 

value (1b-after; Figure 7.8).

Page 46 

Section 7.0 

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vii. Rinse the probe twice with DI water. 


i. Remove the calibration cup and gently pull the pH probe off 

while holding the sonde unit with probes facing down. 



SP135-500, 4 Molar KCl saturated with Silver Chloride). 

iii. While holding the sonde with probes facing down, reattach the 

probe. 

iv. Flip the sonde unit so the probes are facing up and screw the 

calibration cup back on the sonde unit. Rinse twice with pH 7 

solution. 




vi. Record initial pH value (2c-pH before; Figure 7.8). 

vii. Press CALIBRATE, select p:pH, and press ENTER. 

viii. Type in 7.00 for the value of the pH, then press ENTER. Select 

Y to save calibration. The conductivity is calibrated and the unit 

returns to the main menu. Record new pH value (2c-pH after; 

Figure 7.8). 

ix. Rinse the probe twice with DI water. 

x. Rinse the probe twice with pH 10 solution. 

xi. Fill the calibration cup with pH 10 solution (above the D.O. 



xii. Record the initial value (2f-pH before; Figure 7.8). 

xiii. Press CALIBRATE, select p:pH, and press ENTER. 

xiv. Type 10.00 for the value of the pH, then press ENTER. Select Y 

to save calibration. The pH system is calibrated and the unit 

returns to the main screen. Record new pH value (2f-pH after; 

Figure 7.8). 

xv. Rinse the probe twice with DI water. 

xvi. Recheck pH 7 value and recalibrate, if necessary (2h-pH before / 


C. Calibration: Dissolved Oxygen (D.O.)

Page 47 

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i. Before beginning the calibration process, check the D.O. 

membrane. Replace D.O. electrolyte and membrane, if 

necessary. If the D.O. membrane was changed, the unit must sit 


ii. Rinse the probe twice with DI water, then fill the calibration cup 

with DI water so the tip of the D.O. sensor is ~5 mm above the 

liquid surface. 

iii. Blot away any moisture on the membrane surface. 

iv. Place the hard cap on the calibration cup. Allow five minutes 

for water saturation and temperature to equilibrate. At this time, 


seconds. 

v. Record temperature and barometric pressure (760 mm Hg at sea 

level) on the Hydrolab Calibration Sheet (Figure 7.8). 

vi. Check Table 7.4 for D.O. value corresponding with the 

temperature and barometric pressure and record value (3c-Table 

D.O. ; Figure 7.8). 

vii. Let the dissolved oxygen value equilibrate, and record value (3c- 

D.O. before; Figure 7.8). 

viii. Press CALIBRATE and arrow over to O:DO mg/L and press 

ENTER. 

ix. Type in the D.O. value from Table 7-5 and press ENTER. 

x. Select Y to save calibration. The D.O. is calibrated and the unit 

returns to the main screen. Record new D.O. value (3c-D.O. 



i. Empty the calibration cup and leave open (no cap needed). 

Record initial depth (4-Depth before; Figure 7.8). 

ii. Press CALIBRATE, select D:Depth, and press ENTER. 

iii. Type in 0.0 and press ENTER. 

iv. Select Y to save calibration. The depth is calibrated and the unit 

returns to the main menu. Record new depth value (4-Depth 


E. Battery 

i. Record the voltage from the top right corner of the alt screen. 

ii. Change the batteries, if necessary.

Page 48 

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7.3.5.6 Secchi Disk 

Introduction 

A secchi disk provides an easy, economical and reliable measure of water clarity and 

light penetration. Turbidity and available light may be expected to have significant 

effects on the visual perception of fishes, and hence affect catchability. 


203-mm diameter disc with alternating black and white quarters. 

Objective 

To provide a measurement of visibility within the water column that integrates water 

clarity and ambient light level. 

Procedure 

1. This procedure is performed during daytime only. 

2. Locate an area that is free from turbidity generated by the boat or sampling 

operations. Local shadow effects caused by boat, shore vegetation, structures, 

or yourself should also be avoided. Remove sunglasses if worn. During 

nearshore sampling operations (i.e., boat seines, beach seines, haul seines, etc.) 

readings should be taken at maximal depth of gear deployment. 

3. Slowly lower disk into water using distance calibrated (meters and tenths) line. 

When the disk is lost to sight, note the point on the line at water surface. 

4. Lower disk additional 0.2 - 0.3 m, then slowly begin to retrieve to surface. 

When the disk first becomes visible, again note point of line at surface. If this 

varies from the first measurement, average these two values and record to the 

nearest 0.1 m. 

5. If disk settles on bottom before being lost to sight, record water depth and ‘Y’ 

in appropriate boxes on data sheet (‘N’ otherwise). 

7.3.6 Laboratory Work-Up 

7.3.6.1 Representative and Unidentified Sample Work-Up and Reference 

Collection. 

Introduction 

In the field, some fish are collected that are too small, uncommon or difficult to identify 

to their lowest taxonomic level. Therefore, it is necessary to bring these specimens back

Page 49 

Section 7.0 

April 2008 


to the lab for further identification. Fish are also brought back as representative samples 

to confirm field identifications and to augment the specimen reference collection. 

Objectives 

1. To confirm the accuracy of field identifications. 

2. To identify specimens to the lowest possible taxonomic level. 

Procedure 

1. Species Identification / Representative Samples 

A. Initial ID 

i. Locate samples, usually found in freezers. 

ii. Locate appropriate sample worksheet book (Identification or 

Representative Samples; SRS or River study). 

iii. Record the field number, collection date, gear, and grid from the 

tag in the sample bag in the appropriate location on the 

worksheet (Figures 7.9 and 7.10). 

iv. Identify specimens to the lowest taxa possible. 

v. Record the species identification and corresponding length 

measurements. 

vi. Record the method used to determine the species identification 

in the ‘I.D. Method’ column (i.e., fin ray counts or other 

distinguishing characteristics). 

vii. Record the date of sample work-up and the initials of the 

individual who completed the initial identification. 

viii. Representative samples: Only one identification is required. If 

specimens are difficult to identify, get a second opinion. New 

employees should identify specimens and then have an 

experienced FIM staff member confirm their identifications. 

ix. Identification samples require at least two identifications. 

a. Place the specimen(s) back in the bag with the label and 

place in the ‘To Be Confirmed’ section of the freezer. If 

possible have another individual complete the 

confirmation (see below) instead of re-freezing the 

sample. This not only saves room in the freezer but also 

makes the confirmation easier since the specimen(s) are 

relatively fresh.

Page 50 

Section 7.0 

April 2008 


B. Confirmation: To ensure proper identification of specimens, a 

confirmation identification is needed by a second independent FIM 

staff member. 

i. Remove fish from the ‘To Be Confirmed’ section of the freezer 

and thaw the sample(s). 

ii. Identify specimens to the lowest taxa possible. 

iii. 

Locate the corresponding collection number in the appropriate 

sample worksheet book. 

iv. Determine whether confirmation identification matches initial 

identification. 

a. If the identifications match, record the date of 

confirmation and your initials in the ‘CONFIRM 

INITIAL’ column of the worksheet (Figures 7.9 and 

7.10). 

b. If the identification does not match the original 

identification, the sample must be saved for a third 

independent identification. 

(1) Place the specimen(s) back in the bag with the label 

and place back in the ‘To Be Confirmed’ section of the 

freezer. If possible, have another individual complete 

the third identification instead of refreezing the sample. 

(2) Record the new identification on the same line as 

the original identification. 

(3) Record ‘Needs Third ID’ in the ‘Confirm Initial’ 

column of the worksheet. 

(4) Record the date and initials of the individual 

making the new identification in or near the ‘Confirm 

Initial’ column of the worksheet. 

v. Upon confirmation of a sample, the specimens may be discarded 

unless needed for other research projects or incorporation into 

the reference collection. 

C. The last column on the Representative / Identification Samples 

Worksheet (Field ID) should be left blank. A FIM staff member uses 

this column when completing the fish id tabulation. A FIM staff 

member checks the laboratory identifications against field 

identifications to calculate percent accuracy for a 6-month period. 

2. Reference Collection 

A. Fixing specimens: Specimens should be fixed in 10% formalin for 

about one week, thoroughly rinsed in fresh water, then preserved in

Page 51 

Section 7.0 

April 2008 


7.3.7 Data 

50% isopropyl alcohol. Tags should be attached to all samples in 

formalin, stating ‘in 10% formalin’ and the date of fixation. When 

transferring samples, decant clean used formalin into the reusable 

formalin carboy or discard down the drain, flushing with plenty of 

water. 

Note: Formalin is a carcinogen. Consult safety manual or safety 

officer for proper procedure whenever handling formalin or specimens 

stored in formalin. 

B. Addition to reference collection: After preserving the specimens 

properly, place the sample jar on the shelf within the area designated 

by its family. Each sample jar should be labeled with the field number, 

species name, date collected, and location of the collection. The above 

information along with the standard lengths of each specimen should 

be recorded in the ‘REFERENCE COLLECTION’ book. 

C. Make sure all labels are legible and complete. 

7.3.7.1 Data Processing 

Accurate and timely data entry and processing is vital to the success of the Fisheries- 

Independent Monitoring (FIM) program. Data go through a formal process that includes 

data entry, proofing, editing, re-proofing, re-editing, and check program verification prior 

to being considered complete. All data processing is completed using a MS Access 

database application developed by the FIM program. This relational database allows data 

to be easily shared within HBMP groups and with outside agencies that have an interest 

in the data. 

Data entry always begins with the field data sheet for a given sampling trip. This 

establishes the “Primary Key” (Field Number) that ties all underlying data tables (water 

quality, biological, and habitat data) together. Following entry of the field data, the 

biological data may be entered, and then any subsequent wetlab data may be entered. 

Following data entry, proofing reports that mimic the data sheets are printed. Data 

proofing is conducted by two people, one of whom must be capable of acting as PI on a 

sampling trip. There is much more to proofing than simply verifying that the values for 

each variable were entered correctly. The proofers must be aware of the procedures and 

guidelines related to the data and recognize situations where the variables entered do not 

seem reasonable. For example, the proofers should question situations where the wind 

seems too strong, the bank, gear, period or zone seems wrong, or the soaktime or distance 

towed seems excessive. They should spot check the addition for number of fish (e.g., 

was a length left empty and number of fish entered as 20, or were the 20 measured fish 

added into the total?). These are common errors that cannot easily be caught by check 

programs.

Page 52 

Section 7.0 

April 2008 


Errors identified during the proofing phase are corrected using the MS Access database 

application, after which additional proofing reports, for the re-proofing phase, are printed. 

Reproofing can be completed by one person, but should not be the same person who 

made the corrections. This process verifies that the errors identified on the initial 

proofing sheet were corrected during the correction process. Any remaining errors are recorrected 

using the MS Access database application. 

The final phase of the data processing is a series of check programs, written in the 

Statistical Analysis System (SAS). These programs look for data fields that have values 

that seem unlikely. They check against known or expected data ranges, and against 

historical data values. A listing of potential errors is output and researched against both 

the MS Access database and the original data sheets. Identified errors are corrected in the 

MS Access database application.

Page 53 

Section 7.0 

April 2008 


Table 7.1. Summary of Adult and Juvenile Fish HBMP Sampling Element 

Sampling Program Summary for the lower Hillsborough River 

Element # Strata Jan Feb March April May June July August September October November December Total 

Fish - 

Adult / 

Juveniles 

6 strata 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

Sampling program summary for the lower Alafia River 

Fish - 

Adult / 

Juveniles 

6 strata 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

Sampling program summary for McKay Bay 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

Stratum 

2 seines & 

1 trawl / 

Stratum 

144 

seines 

72 

trawls 

144 

seines 

72 

trawls 

Fish - 

Adult / 

Juveniles 

10 cells 

shallow 

4 cells 

deep 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

1 seine/ 

cell 

1 trawl/ 

cell 

120 

seines 

48 

trawls 

Sampling program summary for the TBC/Palm River 

Fish - 

Adult / 

Juveniles 

3 strata 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

Sampling program summary for portion of Hillsborough Bay that is immediately outside of the Alafia River 

2 seines & 

1 trawl / 

stratum 

2 seines 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

stratum 

2 seines & 

1 trawl / 

Stratum 

72 

seines 

36 

trawls 

Fish - 

Adult / 

Juveniles 

2 strata 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

2 seines & 

2 trawls / 

stratum 

48 

seines 

48 

trawls

Page 54 

Section 7.0 

April 2008 


Table 7.2 Gear Deployment And Types 

Shallow Areas (< 1.5m) 

Deep Areas (> 1.8m) 

Indicator 

Sampling Gear 

21-m center bag seine, with 3.2 mm mesh (offshore, 

and boat sets) 

6.1-m Otter trawl with 39-mm stretch mesh and 3.1 

mesh liner 

Table 7.3 Maximum bag depths and wing depths for 21.3-m seine techniques 

Sampling Technique 

Minimum Bag 

(start) Depth (m) 

Maximum Bag 

(start) Depth (m) 

Maximum Wing Depth (m) 

Non-Seawall Seawall 

Set 

Set 

Bay 0.3 1.5 N/A N/A 

River 0.3 1.8 0.3 1.0

Page 55 

Section 7.0 

April 2008 


Table 7.4 Freshwater Oxygen 

Solubility with a Barometric Pressure 

of 760 mm Hg 

Temperature ( 0 C) 

D.O. In mg/L 

19.00 9.24 

19.25 9.20 

19.50 9.15 

19.75 9.10 

20.00 9.06 

20.25 9.02 

20.50 8.97 

20.75 8.92 

21.00 8.88 

21.25 8.84 

21.50 8.80 

21.75 8.75 

22.00 8.71 

22.25 8.67 

22.50 8.63 

22.75 8.59 

23.00 8.55 

23.25 8.51 

23.50 8.47 

23.75 8.43 

24.00 8.39 

24.25 8.35 

24.50 8.31 

24.75 8.28 

25.00 8.24

Page 56 

Section 7.0 

April 2008 


Page 57 

Section 7.0 

April 2008 


Sampling Dates and Locations. Dates & Estimated Time in Area to be 

Sampled. 

Date(S): 

Arrival 

Time:___________________ 

Departure 

Time:_________________ 

Site Description (s): 

Location To Be Sampled (Be Specific) 

(circle types of gear or describe if other) 

Sampling Gear Activity 

70 ft. seine / 600 ft. seine / 600 ft. purse seine / 20 ft. Otter trawl / 400 yd. Trammel net/ 600 

yd trammel net / other: 

Vessel Information 

(circle length) 22 / 24 / 26 ft. Net Skiff 

(circle type of console) Center / Bow 

REGISTRATION #: FL- 

Captain/Field PI: 

FWRI Personnel Information 

Crew/Other Staff: 

1. 4. 

2. 5. 

3. 6. 

FWRI Contact Person: 

Number: 

Form Faxed By: 

Date/Time: 

Section: Fisheries Independent Monitoring 

Figure 7.2 Float plan to be faxed to the Marine Patrol on the morning of sampling

Seiner # 1 (S1) 

Page 58 

Section 7.0 

April 2008 


Bag 

Width 15.5-m 

Pull 9.1-m 

Bag 

S1 

S2 

Pivot pole 

Pivot pole person (P) 

Seiner # 2 (S2) 

A. Center bag seine deployed offshore B. Seine wings brought together and 

pivot pole set 

Bag 

S1 

Bag 

S1 

P 

P 

C. Net wings pulled around pivot pole. 

Seiner # 1 (S1) and pivot pole person 

(P) foot the lead line and push the net 

wings together. 

S2 

D. When top of the bag reaches pivot 

pole, seiner # 2 (S2) stops pulling on 

the net wings. 

S2 

Bag 

P 

S1 

End of bag 

Bag 

S1 

S2 P 

Pivot pole 

S2 

E. Seiner # 2 (S2) pulls lead line of each 

net wing approximately 4 feet to move 

the lead lines at the front of the bag past 

the pivot pole. 

F. Pivot pole person (P) and seiner # 1 

(S1) use the pivot pole to pull the 

seine’s bag opening out of the water. 

Figure 7.3 21.3-m bay seine technique

Figure 7.4. Organism Measurement Techniques 

Page 59 

Section 7.0 

April 2008 


Sample Identification Data 

Location Data 

Latitude 

Page 60 

Section 7.0 

April 2008 


Bay Type Year Month Trip number Seq. number Day Inst. 

Longitude 

Degrees 

Minutes 

Projects Rep Work up 

(S/N) 

PI Crew 1 Crew 2 Crew 3 Crew 4 

Zone Sub Grid 

Gear Stratum Start time (24hr) Start depth (m) 

Station 

Weather 

Direction 

Wind 

Speed 

Clouds Precipitation Tide Minutes Seconds 

Current 

Period 

Water Quality Parameters 

Temperature Dissolved Oxygen Conductivity pH Salinity Secchi Disk 

Depth (m) Inst Inst Inst Inst Inst 

Depth (m) 

On bottom? 

(Y/N) 

Habitat 

Bottom Bottom Vegetation Shore Type Shoreline Bycatch 

Line drawing of sampling area 

% 

% 

4 o 4 o 4 o 

Type 

Type comp Level Type Inund. Over. cover 

1 o 1 o 1 o 

2 o 2 o 2 o 

3 o 3 o 3 o 

Type 

% 

comp 

Percent cover 

Total % shoreline 

covered 

Int. Land to Shore to Shore Type to MHTM 

Distance (meters) from net 

Quantity (gal) 

Seines 

Notes: 

Current relation Wind relation Wing depth (m) 

Event? 

(Y/N) 

Proc. Except.? 

(Y/N) 

Trawls 

Current relation Wind relation End depth (m) 

Soaktime (HH:MM) Bearing Distance towed (nm) 

Trammel Nets 

Gear Damage? 

End time (24hr) Search time (HH:MM) # of panels deployed 

Recorded Reviewed Entered Proofed Corrected Completed 

field data sheet 2005; 7/20/05 

h:\data\data_sheets\field_sheet_2005_two_sided.doc 

Figure 7.5. Field Data Sheet

Page 61 

Section 7.0 

April 2008 


Label Info: Grid Gear Location Page 

Bay Type Year Month Trip Number Seq. number Day Rep Method 

Genus/Species Lengths (mm) Genus/Species Lengths (mm) 

Spp. Spp. 

N Use S ex S C N U se S ex SC 

T ag FH C AH C T ag FH C 

Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Spp. Spp. 



Recorded Reviewed Entered Proofed Corrected Completed Rep Smpl Chk Id Smpl Chk 

Length Data Sheet 2001.1, 01/30/2001, len_data_sht.doc 

Figure 7.6. Length Data Sheet

Page 62 

Section 7.0 

April 2008 


Field Number: 

Date: 

Grid: 

Projects: 

Gear: 

Use: 

FHC: 

PI: 

Species: 

Culled for: 

Rep ID Genetics 

Life history Mercury Len/wt 

Fish Health 

Other: 

Notes: 

Completed: 1 st ID 2 nd ID Final 

Figure 7.7 Sample Labels

Figure 7.8 Hydrolab Calibration Sheet 

Page 63 

Section 7.0 

April 2008 


Figure 7.9 Representative Sample Work Sheet 

Page 64 

Section 7.0 

April 2008 


Figure 7.10 Identification Sample Work Sheet 

Page 65 

Section 7.0 

April 2008 


Appendix 7-A 

Code Lists for Data Collection

Exhibit 7.1 Field Code Lists and 

Gear Descriptions for Fisheries- 

Independent Monitoring 

FIELD NUMBER 

FIELD_NO’s are ten digit variables comprised 

of five distinct parts designed to give every 

sample taken a unique, identifying number. The 

parts are: 

SAMPLING TYPE 

Code Sampling Type 

A Adult Monitoring 

B Bought (09/94) 

C Cryptic Mortality 

D Directed 

Part 

XX 

T 

XXTYY####M 

Description 

The BAY system sampled 

The SAMPLING TYPE 

YY YEAR sample was taken (e.g., 94) 

#### 

M 

Unique identifying collection number for every 

sample taken in a year. For dropnets this 

number is the same for a series of three 

simultaneously dropped nets. For gillnets this 

number is the same for each panel (mesh size) 

in a single net. 

MODIFIER 

F 

G 

H 

K 

M 

N 

O 

P 

R 

S 

V 

V 

Fixed-Station Sampling 

Gear Testing 

Hatchery Release Sampling 

Alafia River Acid Spill Sampling 

Stratified-Random Sampling 

Nutrients 

Oil spill sampling 

Photosynthesis 

Reconnaissance 

Seagrass 

Visual Survey (Used with ‘KY’ 

only) 

River Study (Used with ‘RI’ only) 

BAY 

Code Bay 

AP Apalachicola 

CH Charlotte Harbor 

CK Cedar Key 

EV Everglades (06/96)* 

FW Fort Walton (10/92 – 04/97) 

IR Indian River 

KY Florida Keys 

JX Jacksonville (12/94) 

RI River study 

SR St. Sebastian River 

TB Tampa Bay 

TQ Tequesta (01/97) 

VC 

Volusia County 

Code 

A-E 

L, M, R 

X, Y, Z 

X Extra (09/94) 

Z 

Zoology 

MODIFIER 

Description 

Identifies each panel (mesh size) in a gillnet. A 

is the smallest mesh size in a given net while E 

represents the largest mesh size. 

Designates the Left, Middle, and Right net in a 

series of three dropnets when samples removed 

by the internal seine. 

Correspond to dropnets L, M, and R 

(respectively) when samples removed with a 

dipnet.

GEAR CODES 

Code 


*20 21.3m center-bag seine, 3.1mm mesh, leads spaced every 150mm, offshore-circular set. 

*23 21.3 center-bag seine, 3.1mm mesh, leads spaced every 150mm, boat set. 

*300 6.1m otter trawl w/ 3.1mm liner & tickler chain. A straight tow. 

*301 6.1m otter trawl w/ 3.1mm liner & tickler chain. An arc tow.

Exhibit 7.2 Codes used for instruments 

WATER QUALITY INSTRUMENT 

(INST_TEM, INST_DO, INST_CON, INST_TUR, INST_SAL, and INST_PH) 

Code 

Instrument 

Common codes used at all labs: 

N Nephalometer (02/94) 

R Refractometer (02/94) 

Y YSI SCT-Meter (02/94) 

Z Thermometer (05/96) 

4 PHep pH tester (09/96) 

5 YSI D.O. Meter (10/96) 

6 YSI Model 85 (SCTD-Meter) (10/96) 

Tampa Bay 

A Hydrolab Surveyor 4/H20 Sonde, #23 

B Hydrolab Surveyor 4/Minisonde, #25 

J Hydrolab Scout 2/H20 Sonde, #10 (03/96) 

L Hydrolab Surveyor 3/H20 Sonde, #12 (03/96) 

O Hydrolab Scout 2/Reporter Sonde #15 (03/96) 

T YSI 610D/600 Sonde, #20 (03/96) 

U YSI 610D/600 Sonde, #21 (03/96) 

V Hydrolab Scout 2//Reporter Sonde, #14 (03/96) 

9 Hydrolab Scout 2/H20 Sonde, #22 

Incorporated into the data base on 01/01/94 

TIME 

(STARTIME and ENDTIME in Physical databases) 

Start time, End time, Sunrise, and Sunset are recorded in HH:MM format using the 24 hour clock.

DEPTH 

(STARTDEPTH and ENDDEPTH in Physical databases) 

Start Depth and End Depth are recorded in meters rounded off to the nearest 1/10th. 

LOCATION INSTRUMENT 

(INST_LOC in Physical databases) 

Code 

Instrument 

C Chart (03/96) 

D Differential GPS (02/94) 

G 

GPS (Not Differential) 

(02/94) 

L LORAN (02/94) 


PROJECTS 

Code 

M 

H 

Projects 

Regular FIM program 

sampling 

Hatchery release sampling 

R 

HBMP sampling 

Incorporated into the database on 01/01/00 

DISSOLVED OXYGEN 

(SFST_DO, SFE_DO, BTST_DO and BTE_DO in Physical databases) 

Recorded in milligrams per liter (mg/l) rounded off to the nearest 1/10th. 

TEMPERATURE 

(SFST_TEM, SFE_TEM, BTST_TEM, BTE_TEM in Physical databases) 

Recorded in degrees Celsius ( o C) rounded off to the 

nearest 1/10th. 

SALINITY 

(SFST_SAL, SFE_SAL, BTST_SAL, BTE_SAL in Physical databases) 

Recorded in parts per thousand (λ) rounded off to the nearest 1/10th.

Code 

BOAT 

Boat 

- no boat used (line through field) 

A 24’ Atlas—Voodoo, AP (12/93) 

B 22’ Overton—Jubilee II, TB (09/95) 

C 

23’ Sheffield—CZM, CH 

D 26’ Overton Purse Seine, TB (12/98) 

E 

F 

G 

23’ Sheffield—EC&P, IR 

24’ Tremblay—Fireball, CK 

22’ Overton—Jubilee, TB 

H 22’ Tremblay—Fish Health, CK (12/93) 

I 

J 

22’ Tremblay—Miaz Dragon, TB 

(12/98) 

22’ Tremblay—Miss Joan, IR 

K 24’ Atlas—Rhino, KY (12/93) 

L 

23’ Sheffield—Lightning Rod, CK 

M 26’ World Cat, KY (03/99) 

N 

O 

P 

Q 

R 

22’ Tremblay—Sea Neb, CH 

26’ Franklin Purse—Rhombo, CH 

(06/96) 

24’ Franklin Purse—Pearlfish, AP 

(02/96) 

24’ Overton—Millennium Falcon, TB 

(11/98) 

22’ Tremblay—Road Warrior, TB 

S 19’ Scheffield—Squalus, CK (12/93) 

T 22’ Tremblay—Adult boat, TB (04/95) 

U 22’ Tremblay—Limnatus, TB (12/99) 

V 21’ Parker, KY (03/99) 

W 18’ Parker, KY (03/99) 

X 26’ Atlantic—Cujo, AP (10/96) 

Y 22’ Grey Boat—Mallotus, IR (03/99) 

Z 24’ Overton—Waterdawg, AP (09/96) 

1 18’ Electrofishing Boat, TB (03/99) 

2 24’ Overton, CH (01/00) 

3 17’ Proline, TB (01/00) 

4 Upside-down Tremblay (02/00)

SECCHI DISK 

(SCHI_DSK) 

Depth recorded to the nearest tenth of a meter 


Code 

Y 

N 

BOTTOM 

(SCHI_BOT) 

Definition 

Yes. Secchi disk sighted on bottom 

No. Secchi disk not sighted on bottom 


WIND DIRECTION 

(STWINDDR, EWINDDR) 

Code Direction 

E East 

N North 

NE Northeast 

NW Northwest 

S South 

SE Southeast 

SW Southwest 

W West 

0 No wind 

WIND SPEED 

(STWINDSP, EWINDSP) 

Estimated wind speed recorded in miles per hour (MPH). 

% CLOUD COVER 

(STCLOUDS, ECLOUDS) 

Code 

Percent Cloud Cover 

0 No clouds (clear) 

1-100 Visual estimation 

101 Fog 

102 Rain

Code 

1 

PERIOD 

Period Of The Day 

dawn crepuscular, one hour before sunrise to one hour 

after sunrise 

2 day, one hour after sunrise to one hour before sunset 

3 

dusk crepuscular, one hour before sunset to one hour after 

sunset 

4 night, one hour after sunset to one hour before sunrise 

BOTTOM TYPE 

(B1 and B2 in Physical databases) 

Code 

C 

D 

H 

M 

O 

P 

R 

S 

T 

U 

Bottom Type 

Corals: Hard 

(06/97) 

Detritus 

Shell 

Mud 

Oysters 

Sponge 

Rocks 

Sand 

Tunicates 

Unknown 

BANK 

Bank is defined as the shoreline slope at the land-water interface during a normal high tide. 

Code 

A 

B 

C 

D 

E 

F 

Bank Description 

Gentle sloping bank inundated 

Gentle sloping bank not inundated 

Steep bank inundated 

Steep bank not inundated 

Shoreline that periodically inundates so that the location of 

the bank cannot be determined. This code should be used 

whether or not the shoreline is currently inundated. (10/96) 

Flood conditions. Water is higher than the mean high water 

mark. This code should be used under unusual conditions 

only. (04/98) 

U Unknown. Too far away to tell. (02/96) 


CURRENT RELATION 

(CURRELAT) 

Recorded in degrees from 0-359Ε; 0Ε is into the current, 90Ε is perpendicular to the current and 180Ε is with the current.

WIND RELATION 

(WINRELAT) 

Recorded in degrees from 0-359Ε; 0Ε is into the wind, 90Ε is perpendicular to the wind and 180Ε is with the wind. 

SOAK TIME 

Recorded in HH:MM format representing the total number of hours and minutes that the net has been set. 

DISTANCE TOWED 

(DIST_TOW) 

Recorded in nautical miles (nm), for trawls, using the start and end waypoint recorded by a Loran or GPS. 

CURRENT 

(STCURRNT, ECURRNT) 

Recorded in centimeters per second (cm/s). 

PERCENT COVER 

(P_COVER) 

A visual or tactile estimation of the amount of seagrass or attached algae covering the area sampled. 

Ranges from 0 to 100%. 

BOTTOM VEGETATION 

Code 

A 

B 

C 

F 

(BV1 and BV2 in Physical databases) 

Bottom Vegetation 

Acanthophora spp. 

Acetabularia spp. 

Caulerpa spp. 

Algae: Filamentous red 

Algae: Filamentous 

G 

green 

2 Algae: Mixed 

3 Algae: Unidentified 

4 Seagrasses: Mixed 

5 Seagrasses: Unidentified 

H 

I 

N 

R 

S 

T 

U 

Halodule spp. 

Halophila spp. 

None 

Ruppia spp. 

Syringodium spp. 

Thalassia spp. 

Unknown 

V Valisneria spp. (10/94)

SHORE ORIENTATION 

Code 

0Ε 

90Ε 

180Ε 

(SHORORNT) 

Orientation 

Opening facing shore 

Opening perpendicular to 

shore 

Opening opposite shore 

This variable was not recorded after 07/01/93. 

RATIO 

(BVR1, BVR2, SVR1, SVR2, BYR1, and BYR2 in Physical databases) 

Code Ratio 

0 None 

1 1-10% 

2 11-20% 

3 21-30% 

4 31-40% 

5 41-50% 

6 51-60% 

7 61-70% 

8 71-80% 

9 81-90% 

X 91-100% 


SHORE TYPE RATIO 

If the bank is not 100% covered, this reflects the percentage of the bank covered by each shore type. If the bank is 100% covered, this reflects the 

ratio of the primary to the secondary shore type. The primary and secondary shore type ratios do not have to add up to ten, but cannot be greater 

than ten.

STRATA 

Overhanging Vegetation is defined as a tree or shrub that extends over the water, close (~1-m) to the water surface, so that it provides permanent shade and/or structure during 

a majority of the time to the water through prop roots, branches, or fronds. Floating weed mats, which provide shade and structure to the water, are considered overhanging 

vegetation. Emergent vegetation (Juncus spp., Spartina spp., and cattails) are not classified as overhanging. 

Code 

A 

B 

C 

D 

S 

Offshore seine over grass 

Offshore seine not over grass 

Strata Description 

Boat seine with overhanging vegetation 

Boat seine without overhanging vegetation 

Shoreline habitat sampled using the offshore seine 

technique (01/98) 


SHORE DISTANCE 

(SHOR_DIS in physical databases) 

Distance, recorded to the nearest meter, from the land-water interface to the seine wing closest to shore. When the land-water interface 

cannot be determined because the shoreline is completely inundated (bank type E), the distance is measured from the edge of the shore type to the 

seine wing closest to shore. On sand bars and oyster reefs that are not vegetated, the distance should be recorded from the center of the reef or 

bar. 


BANK DISTANCE 

(BANK_DIS in Physical databases) 

Distance, recorded to the nearest meter, from the mean high water mark to the seine wing closest to shore. When the bank cannot be 

determined because the shoreline completely inundates at high tide (bank type E), the distance is measured from the edge to the shore type to the 

seine wing closest to shore. On sand bars and oyster reefs that are not vegetated, the distance is recorded from the center of the reef or bar. 

Incorporated into the database on 01/01/96

Code 

AP 

AV 

SHORE TYPE 

(SV1 and SV2 in Physical databases) 

Shore Vegetation 

Australian Pines 

Aquatic Vegetation; mixed 

BB Bamboo (12/96) 

BM 

BP 

BW 

CS 

Black Mangrove 

Brazilian Pepper 

Buttonwood 

Cattails 

EL Eleocharis spp. (06/95) 

JU 

Juncus spp. 

LF Leather Fern (12/93) 

MA 

MG 

NO 

OS 

OY 

PA 

RM 

RO 

RR 

Mangrove 

Marsh Grasses 

None 

Overhanging shrubs/trees 

Oysters 

Palmetto 

Red Mangrove 

Rocks 

Rip Rap 

SD Sedge (01/00) 

SG Seagrapes (06/97) 

SN 

SW 

TG 

TO 

TP 

TV 

UN 

WM 

WR 

WX 

Spartina spp. 

Seawall 

Terrestrial Grasses 

Trees: Oak 

Trees: Pine 

Terrestrial Vegetation 

Unknown—Too far away to tell 

(02/96) 

White Mangrove 

Algal mat (Wrack) 

Wax Myrtle 

Halodule (HA), Sand (SA), Unidentified Seagrass (SG), Shell (SH), Mixed Seagrasses (SM), Syringodium (SY), and Thalassia (TH) were omitted from the variable list on 1 Feb 

1996.

WING DEPTH 

(WNG_DPTH in physical databases) 

For boat, beach, offshore, and 183-m seines: the water depth, recorded to the nearest tenth of a meter, where the two seine ends 

were brought together. 

For offshore seines with shoreline strata: the water depth, recorded to the nearest tenth of a meter, of the net wing closest to shore. 

For purse seines: the water depth, recorded to the nearest tenth of a meter, at the deepest part of the purse seine set. Used to 

describe purse seine sets on a slope. 

BEARING 

Directional unit to indicate the direction the trawl was 

towed (0 - 359°)

BYCATCH 

(BY1 and BY2 in Physical databases) 

Code 

Bycatch Type 

AB Acetabularia spp. 

AD Algae: Drift 

AF Algae: Floating Mat 

AG Algae: Filamentous green 

AM Algae: Mixed 

AR Algae: Filamentous red 

AT Acanthophora spp. 

AU Algae: Unknown 

AV Floating aquatic vegetation (01/00) 

BR Bryozoans 

CA Caulerpa spp. 

CG Corals: Gorgonian 

CO Corals: Hard (06/97) 

CH Crabs: Horseshoe 

CI Crabs: Spider 

CL Clay 

CM Crabs: Mixed 

CP Crabs: portunid 

CQ Crab Traps (12/93) 

CS Grasses: Cattails/Marsh 

CT Ctenophores 

DT Detritus 

DW Duck Weed (01/94) 

EM Egg mass: gelatinous 

ES Egg cases: snail 

FT Freshwater turtles (01/96) 

GA Gastropods (03/95) 

GM Grasses: Mixed 

GR Gracilaria spp. 

GU Grasses: Unknown 

HA Halodule spp. 

HI Halophila spp. 

HY Hyacinth 

JF Jellyfish 

JU Juncus spp. 

LL Leaf Litter 

LO Logs (12/98) 

MI Mixed Invertebrates

Code 

MM 

MS 

MU 

NO 

NU 

OC 

OY 

PC 

PN 

PR 

QH 

RO 

RU 

SA 

SD 

SF 

Bycatch Type 

Man-made objects other than crab 

traps.(12/93) 

Mangrove seeds/propogules 

Mud 

None 

Nudibranch/Seahares 

Oil Contaminated sample 

Oysters 

Pine Cones 

Pine Needles 

Palm Root 

Quohogs 

Rocks 

Ruppia spp. 

Sand 

Sand Dollars 

Starfish 

SG Sargassum spp. (06/95) 

SH 

SR 

SN 

SP 

Shell 

Shrimp; noncommercial 

Spartina spp. 

Sponges 

SQ Squid (06/95) 

ST 

SY 

Sticks and branches 

Syringodium spp. 

TA Tubes; amphipod (09/94) 

TD Tadpoles (01/00) 

TH 

TU 

UL 

UR 

UU 

WT 

Thalassia spp. 

Tunicates 

Ulva spp. 

Sea Urchins 

Unknown 

Worm Tubes 

QUANTITY 

Recorded in gallons; an estimation of the amount of 

total bycatch.

Code 

SPLITTER 

(SPLTTYPE in Length database) 

Splitter Type 

2 Two-way splitter 

3 Three-way splitter 

5 

Twenty-five-way 

splitter 

6 Six-way splitter 

SEX 

Code 

Sex 

. No attempt made to determine sex 

F Female 

L Leptocephalus Larvae 

M Male 

N Sex not checked, but should have been. 

P Parasitized crabs 

U Unsexed; sex could not be determined. 


SIZE CLASS (SC) 

Alphabetic variable designed to identify different size classes of a single species. A designates the first size class measured, B designates 

the second size class, etc. 

Code 

C 

USE 

(Length database) 

Use Description 

Fish Culled for various studies, random selected 

G Fish culled for genetics only (11/99) 

I 

M 

N 

R 

S 

T 

UnIdentified specimen 

Fish dead when measured (Mortality) 

Fish culled for various studies, non-randomly selected 

(10/96) 

Fish Recaptured with tag 

Fish kept for representative Sample 

Fish Tagged and released 

Incorporated into the data base on 06/25/94

Code 

FISH HEALTH CODES “FHC” 

Fhc Description 

B Animal with red or bloody areas (5/98) 

Animal with scale loss or erosion (erosion = epidermis or 

E 

dermis involved, muscle tissue not involved) (06/99) 

Animal with fin erosion (fin rot = inflammation of fins, 

F 

frayed fins) (5/98) 

Other (animal with conditions not specifically listed – 

O 

describe condition in comments) (06/99) 

Animal with skeletal malformation (vertebral, opercular, or 

S 

fin deformities) (06/99) 

T Animal with raised area (tumor, cyst) (06/99) 

Animal with depressed area (ulcer or lesion with 

U inflammation, redness, swelling—muscle tissue involved) 

(4/98)

Exhibit 7.3 - NODC SPECIES CODE LIST 

(Alphabetized on scientific name) 

NODCCODE TSN FIM species code Scientific Name Common Name 

8803020101 165548 Abl. hians Ablennes hians Flat needlefish 

8803020100 165547 Ablennes spp. Ablennes spp. 

8835620101 170046 Abu. saxatilis Abudefduf saxatilis Sergeant major 

8835620100 170045 Abudefduf spp. Abudefduf spp. 

8835620102 170047 Abu. taurus Abudefduf taurus Night sergeant 

8835160101 168095 Aca. pomotis Acantharchus pomotis Mud sunfish 

8835160100 168094 Acantharchus spp. Acantharchus spp. 

8842090801 171450 Aca. aspera Acanthemblemaria aspera Roughhead blenny 

8842090803 171452 Aca. chaplini Acanthemblemaria chaplini Papillose blenny 

8842090800 171449 Acanthemblemaria spp. Acanthemblemaria spp. 

8850030601 172451 Aca. solandri Acanthocybium solandri Wahoo 

8850030600 172450 Acanthocybium spp. Acanthocybium spp. 

8860030202 173246 Aca. polygonia Acanthostracion polygonia Honeycomb cowfish 

8860030201 173245 Aca. quadricornis Acanthostracion quadricornis Scrawled cowfish 

8849010000 172250 Acanthuridae spp. Acanthuridae spp. 

8849010101 172252 Aca. bahianus Acanthurus bahianus Ocean surgeon 

8849010102 172253 Aca. chirurgus Acanthurus chirurgus Doctorfish 

8849010103 172254 Aca. coeruleus Acanthurus coeruleus Blue tang 

8849010100 172251 Acanthurus spp. Acanthurus spp. 

8820022502 166616 Ace. dendritica Acentronura dendritica Pipehorse 

8820022500 166613 Acentronura spp. Acentronura spp. 

8858010000 172980 Achiridae spp. Achiridae spp. 

8858030202 172986 Ach. lineatus Achirus lineatus Lined sole 

8858030200 172984 Achirus spp. Achirus spp. 

8729010104 161069 Aci. brevirostrum Acipenser brevirostrum Shortnose sturgeon 

8729010105 573257 Aci. oxyrinchus Acipenser oxyrinchus Atlantic sturgeon 

8729010100 161065 Acipenser spp. Acipenser spp. 

8729010000 161064 Acipenseridae spp. Acipenseridae spp. 

8784010401 164472 Acy. beryllinus Acyrtops beryllinus Emerald clingfish 

8784010400 164471 Acyrtops spp. Acyrtops spp. 

8804040400 165681 Adinia spp. Adinia spp. 

8804040401 165682 Adi. xenica Adinia xenica Diamond killifish 

8713070101 160978 Aet. narinari Aetobatus narinari Spotted eagle ray 

8713070100 160977 Aetobatus spp. Aetobatus spp. 

8836010401 170355 Ago. monticola Agonostomus monticola Mountain mullet 

8836010400 170354 Agonostomus spp. Agonostomus spp. 

8741130101 161421 Ahl. egmontis Ahlia egmontis Key worm eel 

8741130100 161420 Ahlia spp. Ahlia spp.


8739010100 161120 Albula spp. Albula spp. 

8739010101 161121 Alb. vulpes Albula vulpes Bonefish 

8739010000 161119 Albulidae spp. Albulidae spp. 

8835280202 168602 Ale. ciliaris Alectis ciliaris African Pompano 

8835280200 168600 Alectis spp. Alectis spp. 

8762090102 162527 Ale. ferox Alepisaurus ferox 

8707040402 159921 Alo. superciliosus Alopias superciliosus 

8707040401 159916 Alo. vulpinus Alopias vulpinus 

8747010102 161703 Alo. aestivalis Alosa aestivalis Blueback herring 

8747010104 161705 Alo. alabamae Alosa alabamae Alabama shad 

8747010106 161707 Alo. chrysochloris Alosa chrysochloris Skipjack herring 

8747010103 161704 Alo. mediocris Alosa mediocris Hickory shad 

8747010101 161702 Alo. sapidissima Alosa sapidissima American shad 

8747010100 161701 Alosa spp. Alosa spp. 

6179140000 96600 Alpheidae spp. Alpheidae spp. Snapping shrimp 

8835020441 167746 Alp. afer Alphestes afer Mutton hamlet 

8860020102 173132 Alu. heudelotii Aluterus heudelotii Dotterel filefish 

8860020103 173133 Alu. monoceros Aluterus monoceros Unicorn filefish 

8860020101 173131 Alu. schoepfii Aluterus schoepfii Orange filefish 

8860020104 173134 Alu. scriptus Aluterus scriptus Scrawled filefish 

8860020100 173129 Aluterus spp. Aluterus spp. 

6179170300 96962 Ambidexter spp. Ambidexter spp. 

6179170301 96963 Amb. symmetricus Ambidexter symmetricus 

8835160203 168099 Amb. ariommus Ambloplites ariommus Shadow bass 

8835160200 168096 Ambloplites spp. Ambloplites spp. 

8835640101 170224 Amb. pinos Amblycirrhitus pinos Redspotted hawkfish 

8835640100 170223 Amblycirrhitus spp. Amblycirrhitus spp. 

8777020601 164035 Ame. brunneus Ameiurus brunneus Snail bullhead 

8777020602 164037 Ame. catus Ameiurus catus White catfish 

8777020604 164041 Ame. natalis Ameiurus natalis Yellow bullhead 

8777020605 164043 Ame. nebulosus Ameiurus nebulosus Brown bullhead 

8777020607 164047 Ame. serracanthus Ameiurus serracanthus Spotted bullhead 

8777020600 164034 Ameiurus spp. Ameiurus spp. Bullheads 

8734010101 161104 Ami. calva Amia calva Bowfin 

8734010100 161103 Amia spp. Amia spp. 

8734010000 161102 Amiidae spp. Amiidae spp. 

8835200503 168514 Amm. bifascia Ammocrypta bifascia Florida sand darter 

8835200500 168511 Ammocrypta spp. Ammocrypta spp. 

8500010100 159689 Amphioxus spp. Amphioxus spp. Lancelet 

8852010000 172583 Anabantidae spp. Anabantidae spp. 

8713040701 564154 Ana. folirostris Anacanthobatis folirostris 

8713040202 160905 Ana. longirostris Anacanthobatis longirostris


8713040200 160903 Anacanthobatis spp. Anacanthobatis spp. 

8741050107 161169 Ana. similis Anarchias similis Pygmy moray 

8741050100 161161 Anarchias spp. Anarchias spp. 

8820023901 166653 Ana. criniger Anarchopterus criniger Fringed pipefish 

8820023902 166655 Ana. tectus Anarchopterus tectus 

5124000000 78015 Anaspidea spp. Anaspidea spp. Sea hare 

8747020209 161846 Anc. cayorum Anchoa cayorum Key anchovy 

8747020203 161840 Anc. cubana Anchoa cubana Cuban anchovy 

8747020201 161838 Anc. hepsetus Anchoa hepsetus Striped anchovy 

8747020204 161841 Anc. lamprotaenia Anchoa lamprotaenia Bigeye anchovy 

8747020205 161842 Anc. lyolepis Anchoa lyolepis Dusky anchovy 

8747020202 161839 Anc. mitchilli Anchoa mitchilli Bay anchovy 

8747020200 161837 Anchoa spp. Anchoa spp. 

8747020304 161857 Anc. perfasciata Anchoviella perfasciata Flat anchovy 

8857030503 172754 Anc. dilecta Ancylopsetta dilecta Three-eye flounder 

8857030506 172757 Anc. quadrocellata Ancylopsetta quadrocellata Ocellated flounder 

8857030500 172751 Ancylopsetta spp. Ancylopsetta spp. 

8741010101 161127 Ang. rostrata Anguilla rostrata American eel 

8741010100 161126 Anguilla spp. Anguilla spp. 

8741010000 161125 Anguillidae spp. Anguillidae spp. 

8835400300 169080 Anisotremus spp. Anisotremus spp. 

8835400304 169084 Ani. surinamensis Anisotremus surinamensis Black margate 

8835400306 169086 Ani. virginicus Anisotremus virginicus Porkfish 

8774110000 163160 Anostomidae spp. Anostomidae spp. 

8787020000 164518 Antennariidae spp. Antennariidae spp. 

8787020201 164523 Ant. multiocellatus Antennarius multiocellatus Longlure frogfish 

8787020202 164524 Ant. ocellatus Antennarius ocellatus Ocellated frogfish 

8787020208 164530 Ant. pauciradiatus Antennarius pauciradiatus 

8787020203 164525 Ant. radiosus Antennarius radiosus 

8787020200 164522 Antennarius spp. Antennarius spp. 

8787020234 164556 Ant. striatus Antennarius striatus Splitlure frogfish 

8835020702 167771 Ant. nicholsi Anthias nicholsi Yellowfin bass 

8835020700 167769 Anthias spp. Anthias spp. 

8835020703 167772 Ant. tenuis Anthias tenuis Threadnose bass 

8811060101 166311 Ant. capros Antigonia capros Deepbody boarfish 

8811060102 166312 Ant. combatia Antigonia combatia Shortspine boarfish 

8811060100 166310 Antigonia spp. Antigonia spp. 

9002060201 208675 Apa. ferox Apalone ferox Florida softshell 

9002060203 208680 Apa. spinifera Apalone spinifera Spiny softshell 

9002060200 208674 Apalone spp. Apalone spp. Softshell turtles 

8781010000 164403 Aphredoderidae spp. Aphredoderidae spp. 

8781010101 164405 Aph. sayanus Aphredoderus sayanus Pirate perch


8781010100 164404 Aphredoderus spp. Aphredoderus spp. 

8835180101 168198 Apo. affinis Apogon affinis Bigtooth cardinalfish 

8835180104 168201 Apo. aurolineatus Apogon aurolineatus 

8835180105 168202 Apo. binotatus Apogon binotatus Barred cardinalfish 

8835180115 168212 Apo. lachneri Apogon lachneri Whitestar cardinalfish 

8835180125 168224 Apo. leptocaulus Apogon leptocaulus 

8835180107 168204 Apo. maculatus Apogon maculatus Flamefish 

8835180116 168213 Apo. phenax Apogon phenax Mimic cardinalfish 

8835180117 168214 Apo. pillionatus Apogon pillionatus Broadsaddle cardinalfish 

8835180109 168206 Apo. planifrons Apogon planifrons Pale cardinalfish 

8835180110 168207 Apo. pseudomaculatus Apogon pseudomaculatus Twospot cardinalfish 

8835180111 168208 Apo. quadrisquamatus Apogon quadrisquamatus Sawcheek cardinalfish 

8835180100 168197 Apogon spp. Apogon spp. 

8835180113 168210 Apo. townsendi Apogon townsendi Belted cardinalfish 

8835180000 168196 Apogonidae spp. Apogonidae spp. 

8741131601 161490 Apr. platyventris Aprognathodon platyventris Stripe eel 

8741131600 161489 Aprognathodon spp. Aprognathodon spp. 

8835360201 168899 Aps. dentatus Apsilus dentatus 

8741132602 161534 Apt. ansp Apterichtus ansp Academy eel 

8741132603 161536 Apt. kendalli Apterichtus kendalli Finless eel 

8741132600 161531 Apterichtus spp. Apterichtus spp. 

8835430301 169189 Arc. probatocephalus Archosargus probatocephalus Sheepshead 

8835430302 169190 Arc. rhomboidalis Archosargus rhomboidalis Sea bream 

8835430300 169188 Archosargus spp. Archosargus spp. 

8756010205 162067 Arg. striata Argentina striata 

5509051201 79734 Arg. gibbus Argopecten gibbus 

5509051202 79737 Arg. irradians Argopecten irradians 

5509051203 79742 Arg. nucleus Argopecten nucleus 

5509051200 79732 Argopecten spp. Argopecten spp. 

8777180000 164157 Ariidae spp. Ariidae spp. 

8851020101 172537 Ari. bondi Ariomma bondi Silver-rag 

8851020102 172538 Ari. melanum Ariomma melanum Brown driftfish 

8851020104 172540 Ari. regulus Ariomma regulus Spotted driftfish 

8851020100 172536 Ariomma spp. Ariomma spp. 

8777180202 164165 Ari. felis Ariopsis felis Hardhead catfish 

8777180200 164162 Ariopsis spp. Ariopsis spp. 

8741120205 161349 Ari. anale Ariosoma anale Longtrunk conger 

8741120201 161344 Ari. balearicum Ariosoma balearicum Bandtooth conger 

8741120200 161343 Ariosoma spp. Ariosoma spp. 

8835180201 168285 Ast. alutus Astrapogon alutus 

8835180202 168286 Ast. puncticulatus Astrapogon puncticulatus Blackfin cardinalfish 

8835180200 168284 Astrapogon spp. Astrapogon spp.


8835180203 168287 Ast. stellatus Astrapogon stellatus Conchfish 

8835610101 169772 Ast. ocellatus Astronotus ocellatus Oscar 

8835610100 169771 Astronotus spp. Astronotus spp. 

8840140100 171054 Astroscopus spp. Astroscopus spp. 

8840140102 171056 Ast. y-graecum Astroscopus y-graecum Southern stargazer 

8805020000 165984 Atherinidae spp. Atherinidae spp. 

8805020500 166005 Atherinomorus spp. Atherinomorus spp. 

8805020501 166006 Ath. stipes Atherinomorus stipes Hardhead silverside 

8732010201 201897 Atr. spatula Atractosteus spatula Alligator gar 

8762010101 162371 Aul. filamentosus Aulopus filamentosus 

8819010000 166409 Aulostomidae spp. Aulostomidae spp. 

8819010101 166411 Aul. maculatus Aulostomus maculatus Trumpetfish 

8819010100 166410 Aulostomus spp. Aulostomus spp. 

8850030701 172455 Aux. rochei Auxis rochei Bullet mackerel 

8850030700 172454 Auxis spp. Auxis spp. 

8850030702 172456 Aux. thazard Auxis thazard Frigate mackerel 

8847012104 553409 Awa. banana Awaous banana 

8777180101 164159 Bag. marinus Bagre marinus Gafftopsail catfish 

8777180100 164158 Bagre spp. Bagre spp. 

8835440304 169262 Bai. batabana Bairdiella batabana Blue croaker 

8835440301 169259 Bai. chrysoura Bairdiella chrysoura Silver perch 

8835440305 169263 Bai. sanctaeluciae Bairdiella sanctaeluciae Striped croaker 

8835440300 169258 Bairdiella spp. Bairdiella spp. 

8860020201 173138 Bal. capriscus Balistes capriscus Gray triggerfish 

8860020200 173137 Balistes spp. Balistes spp. 

8860020202 173139 Bal. vetula Balistes vetula Queen triggerfish 

8860020000 173128 Balistidae spp. Balistidae spp. 

8847010801 171815 Bar. ceuthoecus Barbulifer ceuthoecus 

8741130203 161425 Bas. bascanium Bascanichthys bascanium Sooty eel 

8741130201 161423 Bas. scuticaris Bascanichthys scuticaris Whip eel 

8741130200 161422 Bascanichthys spp. Bascanichthys spp. 

8835022402 167868 Bat. mexicanus Bathyanthias mexicanus Yellowtail bass 

8835022400 167866 Bathyanthias spp. Bathyanthias spp. 

8741121301 621137 Bat. bullisi Bathycongrus bullisi 

8741121302 621138 Bat. dubius Bathycongrus dubius 

8741121303 621139 Bat. vicinalis Bathycongrus vicinalis 

8847010901 171818 Bat. curacao Bathygobius curacao Notchtongue goby 

8847010902 171819 Bat. mystacium Bathygobius mystacium Island frillfin 

8847010903 171820 Bat. soporator Bathygobius soporator Frillfin goby 

8847010900 171817 Bathygobius spp. Bathygobius spp. 

8768010101 162829 Bat. marionae Bathytyphlops marionae 

8768010100 162828 Bathytyphlops spp. Bathytyphlops spp.


8826020201 166998 Bel. brachychir Bellator brachychir 

8826020202 166999 Bel. egretta Bellator egretta 

8826020203 167000 Bel. militaris Bellator militaris Horned searobin 

8826020200 166997 Bellator spp. Bellator spp. Searobin 

8804080301 165913 Bel. belizanus Belonesox belizanus Pike killifish 

8804080300 165912 Belonesox spp. Belonesox spp. 

8803020000 165546 Belonidae spp. Belonidae spp. 

8840070101 170997 Bem. anatirostris Bembrops anatirostris Duckbill flathead 

8840070102 170998 Bem. gobioides Bembrops gobioides Goby flathead 

8840070100 170996 Bembrops spp. Bembrops spp. 

8713030201 160840 Ben. marcida Benthobatis marcida 

8713030200 160839 Benthobatis spp. Benthobatis spp. 

8852020101 172611 Bet. splendens Betta splendens 

8852020100 172610 Betta spp. Betta spp. 

8842010000 171124 Blenniidae spp. Blenniidae spp. 

8839010301 170483 Bod. pulchellus Bodianus pulchellus Spotfin hogfish 

8839010302 170484 Bod. rufus Bodianus rufus Spanish hogfish 

8839010300 170482 Bodianus spp. Bodianus spp. 

8847011602 171872 Bol. boqueronensis Bollmannia boqueronensis 

8847011601 171871 Bol. communis Bollmannia communis Ragged goby 

8847011603 171873 Bol. eigenmanni Bollmannia eigenmanni 

8847011600 171870 Bollmannia spp. Bollmannia spp. 

8857030000 172714 Bothidae spp. Bothidae spp. 

8857030601 172759 Bot. lunatus Bothus lunatus Peacock flounder 

8857030603 172761 Bot. ocellatus Bothus ocellatus Eyed flounder 

8857030604 172762 Bot. robinsi Bothus robinsi Twospot flounder 

6179111301 96545 Bra. biunguiculatus Brachycarpus biunguiculatus twoclaw shrimp 

6179111300 96544 Brachycarpus spp. Brachycarpus spp. 

8835710102 170290 Bra. brama Brama brama Atlantic pomfret 

8835710103 170291 Bra. caribbea Brama caribbea Caribbean pomfret 

8835710104 170292 Bra. dussumieri Brama dussumieri Lowfin pomfret 

8835710100 170288 Brama spp. Brama spp. 

8835710000 170287 Bramidae spp. Bramidae spp. 

8791020101 164696 Bre. atlanticus Bregmaceros atlanticus 

8791020105 164700 Bre. houdei Bregmaceros houdei 

8747010403 161734 Bre. patronus Brevoortia patronus Gulf menhaden 

8747010404 161735 Bre. smithi Brevoortia smithi Yellowfin menhaden 

8747010400 161731 Brevoortia spp. Brevoortia spp. 

8747010401 161732 Bre. tyrannus Brevoortia tyrannus Atlantic menhaden 

8792010401 164818 Bro. barbata Brotula barbata Bearded brotula 

8792010400 164817 Brotula spp. Brotula spp. 

8820024001 166658 Bry. dunckeri Bryx dunckeri Pugnose pipefish


8792040000 165140 Bythitidae spp. Bythitidae spp. 

8794010405 165373 Cae. caelorhincus Caelorinchus caelorhincus 

8794010401 165364 Cae. caribbaeus Caelorinchus caribbaeus 

8835430501 169196 Cal. arctifrons Calamus arctifrons Grass porgy 

8835430502 169197 Cal. bajonado Calamus bajonado Jolthead porgy 

8835430503 169198 Cal. calamus Calamus calamus Saucereye porgy 

8835430505 169200 Cal. leucosteus Calamus leucosteus Whitebone porgy 

8835430506 169201 Cal. nodosus Calamus nodosus Knobbed porgy 

8835430510 169205 Cal. penna Calamus penna Sheepshead porgy 

8835430507 169202 Cal. pennatula Calamus pennatula 

8835430508 169203 Cal. proridens Calamus proridens Littlehead porgy 

8835430500 169195 Calamus spp. Calamus spp. 

6186020101 98342 Cal. flammea Calappa flammea Flame box crab 

8741130307 161435 Cal. guineensis Callechelys guineensis Shorttail snake eel 

8741130301 161427 Cal. muraena Callechelys muraena Blotched snake eel 

8741130300 161426 Callechelys spp. Callechelys spp. 

8741130305 161431 Cal. springeri Callechelys springeri Ridgefin eel 

8777290000 164279 Callichthyidae spp. Callichthyidae spp. 

6189010308 98703 Cal. bocourti Callinectes bocourti Bocourt swimming crab 

6189010304 98699 Cal. ornatus Callinectes ornatus Shelligs 

6189010301 98696 Cal. sapidus Callinectes sapidus Blue crab 

6189010302 98697 Cal. similis Callinectes similis Lesser blue crab 

6189010300 98695 Callinectes spp. Callinectes spp. 

8846010000 171691 Callionymidae spp. Callionymidae spp. 

8860020401 173157 Can. macrocerus Cantherhines macrocerus Whitespotted filefish 

8860020402 173158 Can. pullus Cantherhines pullus Orangespotted filefish 

8860020400 173156 Cantherhines spp. Cantherhines spp. 

8860020501 173169 Can. maculata Canthidermis maculata Rough triggerfish 

8860020500 173168 Canthidermis spp. Canthidermis spp. 

8860020502 173170 Can. sufflamen Canthidermis sufflamen Ocean triggerfish 

8861010419 621162 Can. jamestyleri Canthigaster jamestyleri 

8861010401 173320 Can. rostrata Canthigaster rostrata Sharpnose puffer 

8861010400 173319 Canthigaster spp. Canthigaster spp. 

8741131701 161492 Car. loxochila Caralophia loxochila Slantlip eel 

8741131700 161491 Caralophia spp. Caralophia spp. 

8835280000 168584 Carangidae spp. Carangidae spp. 

8835280301 168606 Car. bartholomaei Caranx bartholomaei Yellow jack 

8835280306 168612 Car. crysos Caranx crysos Blue runner 

8835280303 168609 Car. hippos Caranx hippos Crevalle jack 

8835280304 168610 Car. latus Caranx latus Horse-eye jack 

8835280307 168613 Car. lugubris Caranx lugubris 

8835280308 168614 Car. ruber Caranx ruber Bar jack


8835280300 168605 Caranx spp. Caranx spp. 

8792020000 165094 Carapidae spp. Carapidae spp. 

8792020101 165096 Car. bermudensis Carapus bermudensis Pearlfish 

8792020100 165095 Carapus spp. Carapus spp. 

8776010301 163350 Car. auratus Carassius auratus Goldfish 

8776010300 163349 Carassius spp. Carassius spp. 

8708020000 160178 Carcharhinidae spp. Carcharhinidae spp. 

8708020504 160304 Car. acronotus Carcharhinus acronotus Blacknose shark 

8708020505 160307 Car. altimus Carcharhinus altimus 

8708020530 160401 Car. brevipinna Carcharhinus brevipinna Spinner shark 

8708020506 160310 Car. falciformis Carcharhinus falciformis Silky shark 

8708020515 160345 Car. galapagensis Carcharhinus galapagensis 

8708020531 160409 Car. isodon Carcharhinus isodon Finetooth shark 

8708020502 160275 Car. leucas Carcharhinus leucas Bull shark 

8708020507 160318 Car. limbatus Carcharhinus limbatus Blacktip shark 

8708020508 160330 Car. longimanus Carcharhinus longimanus Oceanic whitetip 

8708020501 160268 Car. obscurus Carcharhinus obscurus Dusky shark 

8708020511 160336 Car. perezii Carcharhinus perezii Reef shark 

8708020503 160289 Car. plumbeus Carcharhinus plumbeus Sandbar shark 

8708020512 160340 Car. porosus Carcharhinus porosus Smalltail shark 

8708020532 160413 Car. signatus Carcharhinus signatus 

8708020500 160267 Carcharhinus spp. Carcharhinus spp. 

8707030202 159888 Car. taurus Carcharias taurus Sand tiger shark 

8707040101 159903 Car. carcharias Carcharodon carcharias White shark 

8707040100 159902 Carcharodon spp. Carcharodon spp. 

9002040101 173830 Car. caretta Caretta caretta Loggerhead sea turtle 

9002040100 173829 Caretta spp. Caretta spp. Loggerhead sea turtles 

8776040201 163917 Car. cyprinus Carpiodes cyprinus Quillback 

8776040200 163916 Carpiodes spp. Carpiodes spp. 

8776040203 163920 Car. velifer Carpiodes velifer Highfin carpsucker 

8776040000 163892 Catostomidae spp. Catostomidae spp. 

8835220105 168544 Cau. chrysops Caulolatilus chrysops 

8835220102 168541 Cau. cyanops Caulolatilus cyanops Blackline tilefish 

8835220103 168542 Cau. intermedius Caulolatilus intermedius 

8835220104 168543 Cau. microps Caulolatilus microps Blueline tilefish 

8835220100 168539 Caulolatilus spp. Caulolatilus spp. 

8835160000 168093 Centrarchidae spp. Centrarchidae spp. 

8835160301 168102 Cen. macropterus Centrarchus macropterus Flier 

8835160300 168101 Centrarchus spp. Centrarchus spp. 

8819040000 166433 Centriscidae spp. Centriscidae spp. 

8851010000 172508 Centrolophidae spp. Centrolophidae spp. 

8710010301 160633 Cen. granulosus Centrophorus granulosus


8710010303 160637 Cen. uyato Centrophorus uyato 

8788070000 164650 Centrophrynidae spp. Centrophrynidae spp. 

8835010000 167642 Centropomidae spp. Centropomidae spp. 

8835010102 167645 Cen. ensiferus Centropomus ensiferus Swordspine snook 

8835010103 167646 Cen. parallelus Centropomus parallelus Fat snook 

8835010104 167647 Cen. pectinatus Centropomus pectinatus Tarpon snook 

8835010100 167643 Centropomus spp. Centropomus spp. 

8835010105 167648 Cen. undecimalis Centropomus undecimalis Snook 

8835020302 167688 Cen. fuscula Centropristis fuscula Twospot sea bass 

8835020304 167690 Cen. ocyurus Centropristis ocyurus bank sea bass 

8835020305 167691 Cen. philadelphica Centropristis philadelphica Rock sea bass 

8835020300 167686 Centropristis spp. Centropristis spp. 

8835020301 167687 Cen. striata Centropristis striata Black sea bass 

8835550201 169611 Cen. argi Centropyge argi Cherubfish 

8835550200 169610 Centropyge spp. Centropyge spp. 

8710010901 160703 Cen. fabricii Centroscyllium fabricii 

8710011201 160724 Cen. coelolepis Centroscymnus coelolepis 

8835024611 181220 Cep. cruentata Cephalopholis cruentata Graysby 

8835020438 167740 Cep. fulva Cephalopholis fulva Coney 

8847060301 172169 Cer. floridana Cerdale floridana Pugjaw wormfish 

8847060300 172168 Cerdale spp. Cerdale spp. 

8707120101 159907 Cet. maximus Cetorhinus maximus Basking shark 

8707120100 159906 Cetorhinus spp. Cetorhinus spp. 

8842100000 171550 Chaenopsidae spp. Chaenopsidae spp. 

8842091003 171469 Cha. limbaughi Chaenopsis limbaughi Yellowface pikeblenny 

8842091002 171468 Cha. ocellata Chaenopsis ocellata Bluethroat pikeblenny 

8842091004 171470 Cha. roseola Chaenopsis roseola 

8842091000 171466 Chaenopsis spp. Chaenopsis spp. 

8835520101 169539 Cha. faber Chaetodipterus faber Atlantic spadefish 

8835520100 169538 Chaetodipterus spp. Chaetodipterus spp. 

8835550103 169558 Cha. capistratus Chaetodon capistratus Foureye butterflyfish 

8835550101 169556 Cha. ocellatus Chaetodon ocellatus Spotfin butterflyfish 

8835550107 169562 Cha. sedentarius Chaetodon sedentarius Reef butterflyfish 

8835550100 169555 Chaetodon spp. Chaetodon spp. 

8835550108 169563 Cha. striatus Chaetodon striatus Banded butterflyfish 

8835550000 169554 Chaetodontidae spp. Chaetodontidae spp. 

8821010102 166663 Cha. marulius Channa marulius 

8774010000 162848 Characidae spp. Characidae spp. 

6189011101 199969 Cha. hellerii Charybdis hellerii 

6189011100 199968 Charybdis spp. Charybdis spp. 

8857030701 172773 Cha. lugubris Chascanopsetta lugubris 

8842010301 171164 Cha. bosquianus Chasmodes bosquianus Striped blenny


8842010303 621155 Cha. longimaxilla Chasmodes longimaxilla 

8842010302 171165 Cha. saburrae Chasmodes saburrae Florida blenny 

8842010300 171163 Chasmodes spp. Chasmodes spp. 

8787030102 164572 Cha. stigmaeus Chaunax stigmaeus 

8803010103 616680 Che. cyanopterus Cheilopogon cyanopterus Margined flyingfish 

8803010104 165437 Che. exsiliens Cheilopogon exsiliens Bandwing flyingfish 

8803010105 616681 Che. furcatus Cheilopogon furcatus Spotfin flyingfish 

8803010114 616682 Che. melanurus Cheilopogon melanurus Atlantic flyingfish 

9002040201 173833 Che. mydas Chelonia mydas Green sea turtle 

9002040200 173832 Chelonia spp. Chelonia spp. Green sea turtles 

9002040000 173828 Cheloniidae spp. Cheloniidae spp. Sea turtles 

9002010101 208558 Che. serpentina osceola Chelydra serpentina osceola Florida snapping turtle 

9002010100 173751 Chelydra spp. Chelydra spp. Snapping turtles 

9002010000 173750 Chelydridae spp. Chelydridae spp. Snapping turtles 

8861030102 173385 Chi. antennatus Chilomycterus antennatus Bridled burrfish 

8861030103 173386 Chi. antillarum Chilomycterus antillarum Web burrfish 

8861030104 615845 Chi. atringa Chilomycterus atringa Spotted burrfish 

8861030101 615846 Chi. schoepfii Chilomycterus schoepfii Striped burrfish 

8861030100 173383 Chilomycterus spp. Chilomycterus spp. 

8741040100 161148 Chilorhinus spp. Chilorhinus spp. 

8741040101 161149 Chi. suensonii Chilorhinus suensonii Seagrass eel 

8737010000 621135 Chi. ornata Chitala ornata 

8741040000 161147 Chlopsidae spp. Chlopsidae spp. 

8741040501 161158 Chl. bicolor Chlopsis bicolor Bicolor eel 

8741040500 161157 Chlopsis spp. Chlopsis spp. 

8762040101 162430 Chl. agassizi Chlorophthalmus agassizi 

8835280401 168670 Chl. chrysurus Chloroscombrus chrysurus Atlantic bumper 

8835280400 168669 Chloroscombrus spp. Chloroscombrus spp. 

8803010401 165499 Chr. atherinoides Chriodorus atherinoides Hardhead halfbeak 

8803010400 165498 Chriodorus spp. Chriodorus spp. 

8847011703 621159 Chr. vespa Chriolepis vespa 

8835620301 170080 Chr. cyanea Chromis cyanea Blue chromis 

8835620302 613242 Chr. enchrysura Chromis enchrysura Yellowtail reeffish 

8835620303 170082 Chr. insolata Chromis insolata Sunshinefish 

8835620305 170084 Chr. multilineata Chromis multilineata Brown chromis 

8835620307 170086 Chr. scotti Chromis scotti Purple reeffish 

8835620300 170079 Chromis spp. Chromis spp. 

8835610901 169857 Cic. ocellaris Cichla ocellaris 

8835610902 169858 Cic. temensis Cichla temensis 

8835610201 169775 Cic. bimaculatum Cichlasoma bimaculatum Black acara 

8835610224 201999 Cic. citrinellum Cichlasoma citrinellum 

8835610202 169776 Cic. cyanoguttatum Cichlasoma cyanoguttatum Rio grande cichlid


8835610207 169784 Cic. managuense Cichlasoma managuense Jaguar guapote 

8835610208 169786 Cic. meeki Cichlasoma meeki 

8835610203 169777 Cic. nigrofasciatum Cichlasoma nigrofasciatum Convict cichlid 

8835610204 169779 Cic. octofasciatum Cichlasoma octofasciatum Jack dempsey 

8835610209 169788 Cic. salvini Cichlasoma salvini 

8835610200 169774 Cichlasoma spp. Cichlasoma spp. 

8835610221 169802 Cic. urophthalmus Cichlasoma urophthalmus Mayan cichlid 

8835610000 169770 Cichlidae spp. Cichlidae spp. 

8710011902 621033 Cir. asper Cirrhigaleus asper 

8835640000 170222 Cirrhitidae spp. Cirrhitidae spp. 

8857030104 172719 Cit. arctifrons Citharichthys arctifrons Gulf stream flounder 

8857030105 172720 Cit. arenaceus Citharichthys arenaceus Sand whiff 

8857030106 172721 Cit. cornutus Citharichthys cornutus Horned whiff 

8857030107 172722 Cit. dinoceros Citharichthys dinoceros 

8857030108 172723 Cit. gymnorhinus Citharichthys gymnorhinus Anglefin whiff 

8857030109 172724 Cit. macrops Citharichthys macrops Spotted whiff 

8857030110 172725 Cit. spilopterus Citharichthys spilopterus Bay whiff 

8857030100 172715 Citharichthys spp. Citharichthys spp. 

8777120101 164120 Cla. batrachus Clarias batrachus Walking catfish 

8777120100 164119 Clarias spp. Clarias spp. 

8777120000 164118 Clariidae spp. Clariidae spp. 

8839010401 170496 Cle. parrae Clepticus parrae Creole wrasse 

8839010400 170495 Clepticus spp. Clepticus spp. 

8747010000 161700 Clupeidae spp. Clupeidae spp. 

8741120101 161326 Con. oceanicus Conger oceanicus Conger eel 

8741120100 161325 Conger spp. Conger spp. 

8741120102 161327 Con. triporiceps Conger triporiceps Manytooth conger 

8741120000 161324 Congridae spp. Congridae spp. 

8835400401 169090 Con. nobilis Conodon nobilis Barred grunt 

8835400400 169089 Conodon spp. Conodon spp. 

8835170302 168195 Coo. japonicus Cookeolus japonicus 

8810080601 166251 Cor. spinosus Corniger spinosus 

8835290102 168792 Cor. equiselis Coryphaena equiselis Pompano dolphin 

8835290101 168791 Cor. hippurus Coryphaena hippurus Dolphin 

8835290100 168790 Coryphaena spp. Coryphaena spp. 

8847010202 171751 Cor. alloides Coryphopterus alloides Barfin goby 

8847010203 171752 Cor. dicrus Coryphopterus dicrus Colon goby 

8847010204 171753 Cor. eidolon Coryphopterus eidolon Pallid goby 

8847010205 171754 Cor. glaucofraenum Coryphopterus glaucofraenum Bridled goby 

8847010206 171755 Cor. hyalinus Coryphopterus hyalinus Glass goby 

8847010207 171756 Cor. lipernes Coryphopterus lipernes Peppermint goby 

8847010208 171757 Cor. personatus Coryphopterus personatus Masked goby


8847010209 171758 Cor. punctipectophorus 

Coryphopterus 

punctipectophorus 

Spotted goby 

8847010200 171749 Coryphopterus spp. Coryphopterus spp. 

8847010210 171759 Cor. thrix Coryphopterus thrix Bartail goby 

8847010211 171760 Cor. tortugae Coryphopterus tortugae 

8820022301 166597 Cos. albirostris Cosmocampus albirostris Whitenose pipefish 

8820022303 166601 Cos. brachycephalus Cosmocampus brachycephalus Crested pipefish 

8820022305 166605 Cos. elucens Cosmocampus elucens 

8820022306 166607 Cos. hildebrandi Cosmocampus hildebrandi 

8820022302 166599 Cos. profundus Cosmocampus profundus Deepwater pipefish 

8820022300 166596 Cosmocampus spp. Cosmocampus spp. 

8713040400 160917 Cruriraja spp. Cruriraja spp. 

8713040401 160918 Cru. atlantis Cruriraja atlantis 

8713040402 160919 Cru. poeyi Cruriraja poeyi 

8713040403 160920 Cru. rugosa Cruriraja rugosa 

8839030201 170857 Cry. roseus Cryptotomus roseus Bluelip parrotfish 

8839030200 170856 Cryptotomus spp. Cryptotomus spp. 

8835161101 201982 Cry. asprella Crystallaria asprella Crystal darter 

8847010501 171768 Cte. boleosoma Ctenogobius boleosoma Darter goby 

8847013603 621160 Cte. fasciatus Ctenogobius fasciatus 

8847010513 171780 Cte. pseudofasciatus Ctenogobius pseudofasciatus Slashcheek goby 

8847010510 171777 Cte. saepepallens Ctenogobius saepepallens Dash goby 

8847010504 171772 Cte. shufeldti Ctenogobius shufeldti Freshwater goby 

8847010511 171778 Cte. smaragdus Ctenogobius smaragdus Emerald goby 

8847013600 171926 Ctenogobius spp. Ctenogobius spp. 

8847010506 171774 Cte. stigmaticus Ctenogobius stigmaticus Marked goby 

8847010507 171775 Cte. stigmaturus Ctenogobius stigmaturus Spottail goby 

8776012301 163537 Cte. idella Ctenopharyngodon idella Grass carp 

8776012300 163536 Ctenopharyngodon spp. Ctenopharyngodon spp. 

8851020202 172546 Cub. pauciradiatus Cubiceps pauciradiatus Bigeye cigarfish 

8851020200 172545 Cubiceps spp. Cubiceps spp. 

8857030801 172776 Cyc. chittendeni Cyclopsetta chittendeni Mexican flounder 

8857030802 172777 Cyc. fimbriata Cyclopsetta fimbriata Spotfin flounder 

8857030800 172775 Cyclopsetta spp. Cyclopsetta spp. 

8858020000 173060 Cynoglossidae spp. Cynoglossidae spp. 

8835440106 169243 Cyn. arenarius Cynoscion arenarius Sand seatrout 

8835440102 169239 Cyn. nebulosus Cynoscion nebulosus Spotted seatrout 

8835440103 169240 Cyn. nothus Cynoscion nothus Silver seatrout 

8835440104 169241 Cyn. regalis Cynoscion regalis Atlantic weakfish 

8835440100 169238 Cynoscion spp. Cynoscion spp. 

8776017605 163774 Cyp. callitaenia Cyprinella callitaenia Bluestripe shiner 

8776017612 163788 Cyp. leedsi Cyprinella leedsi Bannerfin shiner


8776017600 163765 Cyprinella spp. Cyprinella spp. 

8776017621 163809 Cyp. venusta Cyprinella venusta Blacktail shiner 

8776010000 163342 Cyprinidae spp. Cyprinidae spp. 

8804040100 165630 Cyprinodon spp. Cyprinodon spp. 

8804040101 165631 Cyp. variegatus Cyprinodon variegatus Sheepshead minnow 

8804040000 165629 Cyprinodontidae spp. Cyprinodontidae spp. 

8776010101 163344 Cyp. carpio Cyprinus carpio Common carp 

8776010100 163343 Cyprinus spp. Cyprinus spp. 

8803010102 165435 Cyp. comatus Cypselurus comatus Clearwing flyingfish 

8803010100 165432 Cypselurus spp. Cypselurus spp. 

8713040501 160922 Dac. armatus Dactylobatus armatus 

8713040500 160921 Dactylobatus spp. Dactylobatus spp. 

8832010000 167622 Dactylopteridae spp. Dactylopteridae spp. 

8832010100 167623 Dactylopterus spp. Dactylopterus spp. 

8832010101 167624 Dac. volitans Dactylopterus volitans Flying gurnard 

8840130000 171036 Dactyloscopidae spp. Dactyloscopidae spp. 

8840130202 171043 Dac. crossotus Dactyloscopus crossotus Bigeye stargazer 

8840130205 621154 Dac. foraminosus Dactyloscopus foraminosus 

8840130204 171045 Dac. moorei Dactyloscopus moorei Speckled stargazer 

8840130200 171041 Dactyloscopus spp. Dactyloscopus spp. 

8840130201 171042 Dac. tridigitatus Dactyloscopus tridigitatus Sand stargazer 

8710010401 160651 Dal. licha Dalatias licha 

8713050000 160946 Dasyatidae spp. Dasyatidae spp. 

8713050103 160951 Das. americana Dasyatis americana Southern stingray 

8713050104 160952 Das. centroura Dasyatis centroura Roughtail stingray 

8713050105 160953 Das. sabina Dasyatis sabina Atlantic stingray 

8713050106 160954 Das. say Dasyatis say Bluntnose stingray 

8713050100 160947 Dasyatis spp. Dasyatis spp. 

8835281201 168724 Dec. macarellus Decapterus macarellus Mackerel scad 

8835281202 168725 Dec. punctatus Decapterus punctatus Round scad 

8835281200 168723 Decapterus spp. Decapterus spp. 

8835281203 168726 Dec. tabl Decapterus tabl 

8839010501 170498 Dec. puellaris Decodon puellaris 

9002030501 173786 Dei. reticularia Deirochelys reticularia Chicken turtle 

9002030500 173785 Deirochelys spp. Deirochelys spp. Chicken turtles 

8835020440 167744 Der. inermis Dermatolepis inermis Marbled grouper 

9002050000 173841 Dermochelyidae spp. Dermochelyidae spp. Leatherback sea turtles 

9002050101 173843 Der. coriacea Dermochelys coriacea Leatherback sea turtle 

9002050100 173842 Dermochelys spp. Dermochelys spp. Leatherback sea turtles 

8815020201 166350 Des. polystictum Desmodema polystictum 

8835390204 169029 Dia. auratus Diapterus auratus Irish pompano 

8835390202 169027 Dia. rhombeus Diapterus rhombeus


8835390200 169026 Diapterus spp. Diapterus spp. 

8861030202 173392 Dio. holocanthus Diodon holocanthus Balloonfish 

8861030201 173391 Dio. hystrix Diodon hystrix Porcupinefish 

8861030200 173390 Diodon spp. Diodon spp. 

8861030000 173382 Diodontidae spp. Diodontidae spp. 

8835021005 167796 Dip. bivittatum Diplectrum bivittatum Dwarf sand perch 

8835021002 167793 Dip. formosum Diplectrum formosum Sand perch 

8835021000 167791 Diplectrum spp. Diplectrum spp. 

8835430402 169193 Dip. argenteus Diplodus argenteus Silver porgy 

8835430401 169192 Dip. holbrookii Diplodus holbrookii Spottail pinfish 

8835430400 169191 Diplodus spp. Diplodus spp. 

8846010702 171737 Dip. pauciradiatus Diplogrammus pauciradiatus Spotted dragonet 

8846010700 171735 Diplogrammus spp. Diplogrammus spp. 

8850011001 172376 Dip. multistriatus Diplospinus multistriatus 

8713040160 564127 Dip. bullisi Dipturus bullisi 

8713040127 564146 Dip. olseni Dipturus olseni Spreadfin skate 

8839010601 170500 Dor. megalepis Doratonotus megalepis Dwarf wrasse 

8839010600 170499 Doratonotus spp. Doratonotus spp. 

8847013302 171919 Dor. maculatus Dormitator maculatus Fat sleeper 

8847013300 171917 Dormitator spp. Dormitator spp. 

8747010501 161737 Dor. cepedianum Dorosoma cepedianum Gizzard shad 

8747010502 161738 Dor. petenense Dorosoma petenense Threadfin shad 

8747010500 161736 Dorosoma spp. Dorosoma spp. 

8741170101 161597 Dys. anguillare Dysomma anguillare Shortbelly eel 

8741170100 161596 Dysomma spp. Dysomma spp. 

8835270000 168567 Echeneidae spp. Echeneidae spp. 

8835270201 168575 Ech. naucrates Echeneis naucrates Sharksucker 

8835270202 168576 Ech. neucratoides Echeneis neucratoides Whitefin sharksucker 

8835270200 168574 Echeneis spp. Echeneis spp. 

8741050201 161171 Ech. catenata Echidna catenata Chain moray 

8741050200 161170 Echidna spp. Echidna spp. 

8710030101 160713 Ech. brucus Echinorhinus brucus 

8792020204 165118 Ech. dawsoni Echiodon dawsoni 

8741133101 161551 Ech. intertinctus Echiophis intertinctus Spotted spoon-nose eel 

8741133102 161553 Ech. punctifer Echiophis punctifer Stippled spoon-nose eel 

8741133100 161550 Echiophis spp. Echiophis spp. 

8847010605 171793 Ela. horsti Elacatinus horsti Yellowline goby 

8847010607 171795 Ela. macrodon Elacatinus macrodon Tiger goby 

8847010608 171796 Ela. oceanops Elacatinus oceanops Neon goby 

8847010609 171797 Ela. xanthiprora Elacatinus xanthiprora Yellowprow goby 

8835281301 168738 Ela. bipinnulata Elagatis bipinnulata Rainbow runner 

8835281300 168737 Elagatis spp. Elagatis spp.


8835740101 168169 Ela. evergladei Elassoma evergladei Everglades pygmy sunfish 

8835740102 168170 Ela. okefenokee Elassoma okefenokee 

Okefenokee pygmy 

sunfish 

8835740100 168168 Elassoma spp. Elassoma spp. 

8835740103 168171 Ela. zonatum Elassoma zonatum Banded pygmy sunfish 

8835740000 202000 Elassomatidae spp. Elassomatidae spp. 

8847070000 172171 Eleotridae spp. Eleotridae spp. 

8847013708 621157 Ele. amblyopsis Eleotris amblyopsis 

8847013709 621158 Ele. perniger Eleotris perniger 

8847013700 171929 Eleotris spp. Eleotris spp. 

8738010000 161109 Elopidae spp. Elopidae spp. 

8738010101 161111 Elo. saurus Elops saurus Ladyfish 

8738010100 161110 Elops spp. Elops spp. 

8842090201 171404 Emb. atlantica Emblemaria atlantica 

8842090202 171405 Emb. pandionis Emblemaria pandionis Sailfin blenny 

8842090203 171406 Emb. piratula Emblemaria piratula Pirate blenny 

8842090200 171403 Emblemaria spp. Emblemaria spp. 

8842093101 171547 Emb. bahamensis Emblemariopsis bahamensis Blackhead blenny 

8842093102 171549 Emb. diaphana Emblemariopsis diaphana Glass blenny 

8835350101 168830 Emm. atlanticus Emmelichthyops atlanticus Bonnetmouth 

8835350100 168829 Emmelichthyops spp. Emmelichthyops spp. 

9002030000 173769 Emydidae spp. Emydidae spp. Sliders 

8741050901 161266 Enc. anatina Enchelycore anatina 

8741050302 161181 Enc. carychroa Enchelycore carychroa Chestnut moray 

8741050301 161180 Enc. nigricans Enchelycore nigricans Viper moray 

8741050300 161179 Enchelycore spp. Enchelycore spp. 

8791031501 164748 Enc. cimbrius Enchelyopus cimbrius Fourbeard rockling 

8791031500 164747 Enchelyopus spp. Enchelyopus spp. 

8747020000 553173 Engraulidae spp. Engraulidae spp. 

8747020103 161830 Eng. eurystole Engraulis eurystole Silver anchovy 

8747020100 161827 Engraulis spp. Engraulis spp. 

8857030901 172779 Eng. senta Engyophrys senta Spiny flounder 

8857030900 172778 Engyophrys spp. Engyophrys spp. 

8835160401 168108 Enn. chaetodon Enneacanthus chaetodon Blackbanded sunfish 

8835160402 168113 Enn. gloriosus Enneacanthus gloriosus Bluespotted sunfish 

8835160403 168117 Enn. obesus Enneacanthus obesus Banded sunfish 

8835160400 168107 Enneacanthus spp. Enneacanthus spp. 

8842080101 171366 Enn. altivelis Enneanectes altivelis Lofty triplefin 

8842080102 171367 Enn. boehlkei Enneanectes boehlkei Roughhead triplefin 

8842080103 171368 Enn. pectoralis Enneanectes pectoralis Redeye triplefin 

8842080100 171365 Enneanectes spp. Enneanectes spp. 

8842010401 171167 Ent. nigricans Entomacrodus nigricans Pearl blenny


8842010400 171166 Entomacrodus spp. Entomacrodus spp. 

8835520000 169537 Ephippidae spp. Ephippidae spp. 

8835020402 167696 Epi. adscensionis Epinephelus adscensionis Rock hind 

8835020404 167698 Epi. drummondhayi Epinephelus drummondhayi Speckled hind 

8835020405 167699 Epi. flavolimbatus Epinephelus flavolimbatus Yellowedge grouper 

8835020406 167700 Epi. guttatus Epinephelus guttatus Red hind 

8835020401 167695 Epi. itajara Epinephelus itajara Jewfish 

8835020408 167702 Epi. morio Epinephelus morio Red grouper 

8835020409 167703 Epi. mystacinus Epinephelus mystacinus Misty grouper 

8835020410 167704 Epi. nigritus Epinephelus nigritus Warsaw grouper 

8835020411 167705 Epi. niveatus Epinephelus niveatus Snowy grouper 

8835020400 167694 Epinephelus spp. Epinephelus spp. 

8835020412 167706 Epi. striatus Epinephelus striatus Nassau grouper 

8835441202 169314 Equ. lanceolatus Equetus lanceolatus Jackknife-fish 

8835441205 169317 Equ. punctatus Equetus punctatus 

8835441200 169312 Equetus spp. Equetus spp. 

9002040301 173836 Ere. imbricata Eretmochelys imbricata Hawksbill sea turtle 

9002040300 173835 Eretmochelys spp. Eretmochelys spp. Hawksbill sea turtles 

8776040302 163924 Eri. oblongus Erimyzon oblongus 

8776040300 163921 Erimyzon spp. Erimyzon spp. 

8776040301 163922 Eri. sucetta Erimyzon sucetta Lake chubsucker 

8776040303 163926 Eri. tenuis Erimyzon tenuis Sharpfin chubsucker 

8847011801 171878 Ero. smaragdus Erotelis smaragdus Emerald sleeper 

8847011800 171877 Erotelis spp. Erotelis spp. 

8835350403 168840 Ery. monodi Erythrocles monodi Crimson rover 

8835350400 168837 Erythrocles spp. Erythrocles spp. 

8758010000 162137 Esocidae spp. Esocidae spp. 

8758010102 162140 Eso. americanus Esox americanus Pickerel 

8758010103 162143 Eso. niger Esox niger Chain pickerel 

8758010100 162138 Esox spp. Esox spp. 

8835360301 168902 Ete. oculatus Etelis oculatus Queen snapper 

8835360300 168901 Etelis spp. Etelis spp. 

8741132803 161545 Eth. akkistikos Ethadophis akkistikos 

8835200706 201986 Eth. colorosum Etheostoma colorosum 

8835200128 168387 Eth. davisoni Etheostoma davisoni Choctawhatchee darter 

8835200131 168390 Eth. edwini Etheostoma edwini Brown darter 

8835200101 168358 Eth. fusiforme Etheostoma fusiforme Swamp darter 

8835200139 168398 Eth. histrio Etheostoma histrio Harlequin darter 

8835200158 168417 Eth. okaloosae Etheostoma okaloosae Okaloosa darter 

8835200102 168360 Eth. olmstedi Etheostoma olmstedi Tessellated darter 

8835200162 168421 Eth. parvipinne Etheostoma parvipinne Goldstripe darter 

8835200165 168424 Eth. proeliare Etheostoma proeliare Cypress darter


8835200100 168357 Etheostoma spp. Etheostoma spp. 

8835200176 168437 Eth. stigmaeum Etheostoma stigmaeum Speckled darter 

8835200178 168439 Eth. swaini Etheostoma swaini Gulf darter 

8710010509 160669 Etm. gracilispinis Etmopterus gracilispinis 

8710010506 160664 Etm. virens Etmopterus virens 

8857030201 172729 Etr. crossotus Etropus crossotus Fringed flounder 

8857030205 172733 Etr. cyclosquamus Etropus cyclosquamus 

8857030202 172730 Etr. microstomus Etropus microstomus Smallmouth flounder 

8857030204 172732 Etr. rimosus Etropus rimosus Gray flounder 

8857030200 172728 Etropus spp. Etropus spp. 

8747010600 161742 Etrumeus spp. Etrumeus spp. 

8747010601 161743 Etr. teres Etrumeus teres Round herring 

8835390101 169015 Euc. argenteus Eucinostomus argenteus Spotfin mojarra 

8835390102 169016 Euc. gula Eucinostomus gula Silver jenny 

8835390111 169025 Euc. harengulus Eucinostomus harengulus Tidewater mojarra 

8835390108 169022 Euc. havana Eucinostomus havana Bigeye mojarra 

8835390109 169023 Euc. jonesii Eucinostomus jonesii Slender mojarra 

8835390104 169018 Euc. lefroyi Eucinostomus lefroyi Mottled mojarra 

8835390105 169019 Euc. melanopterus Eucinostomus melanopterus Flagfin mojarra 

8835390100 169014 Eucinostomus spp. Eucinostomus spp. 

8835390203 169028 Eug. plumieri Eugerres plumieri Striped mojarra 

8803010600 165502 Euleptorhamphus spp. Euleptorhamphus spp. 

8803010601 165503 Eul. velox Euleptorhamphus velox Flying halfbeak 

8815010201 166338 Eum. fiski Eumecichthys fiski Unicornfish 

8815010200 166337 Eumecichthys spp. Eumecichthys spp. 

8850030102 172402 Eut. alletteratus Euthynnus alletteratus Little tunny 

8850030100 172399 Euthynnus spp. Euthynnus spp. 

8847011002 171826 Eve. spongicola Evermannichthys spongicola Sponge goby 

8847011000 171824 Evermannichthys spp. Evermannichthys spp. 

8847010401 171764 Evo. lyricus Evorthodus lyricus Lyre goby 

8847010400 171763 Evorthodus spp. Evorthodus spp. 

8850020501 172395 Evo. taeniatus Evoxymetopon taeniatus 

8803010000 165431 Exocoetidae spp. Exocoetidae spp. 

8803010701 165507 Exo. obtusirostris Exocoetus obtusirostris 

Oceanic two-wing 

flyingfish 

8803010700 165506 Exocoetus spp. Exocoetus spp. 

8803010702 165508 Exo. volitans Exocoetus volitans 

Tropical two-wing 

flyingfish 

8741100100 161302 Facciolella spp. Facciolella spp. 

6177010101 551570 Far. aztecus Farfantepenaeus aztecus Brown shrimp 

6177010104 551571 Far. brasiliensis Farfantepenaeus brasiliensis 

6177010102 551574 Far. duorarum Farfantepenaeus duorarum Pink shrimp 

6177010100 95604 Farfantepenaeus spp. Farfantepenaeus spp. Commercial shrimp


8713040161 564119 Fen. sinusmexicanus Fenestraja sinusmexicanus 

8819020102 166417 Fis. petimba Fistularia petimba Red cornetfish 

8819020100 166415 Fistularia spp. Fistularia spp. 

8819020101 166416 Fis. tabacaria Fistularia tabacaria Bluespotted cornetfish 

8804040501 165685 Flo. carpio Floridichthys carpio Goldspotted killifish 

8804040500 165684 Floridichthys spp. Floridichthys spp. 

8846010801 171740 Foe. agassizii Foetorepus agassizii Spotfin dragonet 

8846010802 621156 Foe. goodenbeani Foetorepus goodenbeani 

8804040233 201973 Fun. blairae Fundulus blairae 

8804040208 165652 Fun. chrysotus Fundulus chrysotus Golden topminnow 

8804040217 165661 Fun. cingulatus Fundulus cingulatus 

8804040201 165645 Fun. confluentus Fundulus confluentus Marsh killifish 

8804040229 165675 Fun. escambiae Fundulus escambiae 

8804040207 165651 Fun. grandis Fundulus grandis Gulf killifish 

8804040203 165647 Fun. heteroclitus Fundulus heteroclitus Mummichog 

8804040209 165653 Fun. jenkinsi Fundulus jenkinsi Saltmarsh topminnow 

8804040218 165662 Fun. lineolatus Fundulus lineolatus Lined topminnow 

8804040205 165649 Fun. majalis Fundulus majalis Striped killifish 

8804040211 165655 Fun. olivaceus Fundulus olivaceus Blackspotted topminnow 

8804040212 165656 Fun. pulvereus Fundulus pulvereus 

8804040234 201974 Fun. rubrifrons Fundulus rubrifrons 

8804040223 165667 Fun. seminolis Fundulus seminolis Seminole killifish 

8804040213 165657 Fun. similis Fundulus similis Longnose killifish 

8804040200 165644 Fundulus spp. Fundulus spp. Assorted killifish 

8708020201 160189 Gal. cuvier Galeocerdo cuvier Tiger shark 

8708020200 160188 Galeocerdo spp. Galeocerdo spp. 

8708010201 160030 Gal. arae Galeus arae 

8804080101 165878 Gam. affinis Gambusia affinis 

8804080116 165896 Gam. holbrooki Gambusia holbrooki Eastern mosquito fish 

8804080102 165882 Gam. rhizophorae Gambusia rhizophorae Mangrove gambusia 

8804080100 165877 Gambusia spp. Gambusia spp. 

8857031001 172781 Gas. frontalis Gastropsetta frontalis Shrimp flounder 

8857031000 172780 Gastropsetta spp. Gastropsetta spp. 

8850010000 172354 Gempylidae spp. Gempylidae spp. 

8850010201 172360 Gem. serpens Gempylus serpens Snake mackerel 

8850010200 172359 Gempylus spp. Gempylus spp. 

8835612104 169919 Geo. surinamensis Geophagus surinamensis 

8810050000 615855 Gep. darwinii Gephyroberyx darwinii 

8835390000 169013 Gerreidae spp. Gerreidae spp. 

8835390301 169032 Ger. cinereus Gerres cinereus Yellowfin mojarra 

8835390300 169031 Gerres spp. Gerres spp. 

8840130101 171038 Gil. greyae Gillellus greyae Arrow stargazer


8840130104 171040 Gil. healae Gillellus healae 

8840130100 171037 Gillellus spp. Gillellus spp. 

8840130103 171039 Gil. uranidea Gillellus uranidea Warteye stargazer 

8707100101 159977 Gin. cirratum Ginglymostoma cirratum Nurse shark 

8707100100 159976 Ginglymostoma spp. Ginglymostoma spp. 

8756010302 162074 Glo. pygmaeus Glossanodon pygmaeus 

8840140201 171058 Gna. egregius Gnathagnus egregius 

8847011100 171827 Gnatholepis spp. Gnatholepis spp. 

8847011101 171828 Gna. thompsoni Gnatholepis thompsoni Goldspot goby 

8741120402 161361 Gna. bathytopos Gnathophis bathytopos 

8741120403 161362 Gna. bracheatopos Gnathophis bracheatopos 

8784010000 164457 Gobiesocidae spp. Gobiesocidae spp. 

8784010105 164463 Gob. punctulatus Gobiesox punctulatus Stippled clingfish 

8784010100 164458 Gobiesox spp. Gobiesox spp. 

8784010102 164460 Gob. strumosus Gobiesox strumosus Skilletfish 

8847010000 171746 Gobiidae spp. Gobiidae spp. 

8847011201 171832 Gob. broussonetii Gobioides broussonetii Violet goby 

8847011200 171831 Gobioides spp. Gobioides spp. 

8847013401 171921 Gob. dormitor Gobiomorus dormitor Bigmouth sleeper 

8847013400 171920 Gobiomorus spp. Gobiomorus spp. 

8847010503 171769 Gob. oceanicus Gobionellus oceanicus Highfin goby 

8847010500 171767 Gobionellus spp. Gobionellus spp. 

8847010601 171789 Gob. bosc Gobiosoma bosc Naked goby 

8847010602 171790 Gob. ginsurgi Gobiosoma ginsurgi Seaboard goby 

8847010604 171792 Gob. grosvenori Gobiosoma grosvenori Rockcut goby 

8847010606 171794 Gob. longipala Gobiosoma longipala Twoscale goby 

8847010603 171791 Gob. robustum Gobiosoma robustum Code goby 

8847010600 171788 Gobiosoma spp. Gobiosoma spp. 

8847012201 171890 Gob. myersi Gobulus myersi 

8741130503 161438 Gor. ergodes Gordiichthys ergodes 

8741130501 161437 Gor. irretitus Gordiichthys irretitus Horsehair eel 

8741130504 161439 Gor. leibyi Gordiichthys leibyi 

8741130500 161436 Gordiichthys spp. Gordiichthys spp. 

8835060000 168012 Grammatidae spp. Grammatidae spp. 

8811040000 166296 Grammicolepidae spp. Grammicolepidae spp. 

8811040101 166298 Gra. brachiusculus Grammicolepis brachiusculus 

8811040100 166297 Grammicolepis spp. Grammicolepis spp. 

8792042501 164973 Gra. claudei Grammonus claudei Reef-cave brotula 

8792042500 164972 Grammonus spp. Grammonus spp. 

9002030701 173791 Gra. barbouri Graptemys barbouri Barbour's map turtle 

900203070902 173800 Gra. psuedo. kohnii 

Graptemys pseudogeographica 

kohnii


9002030700 173790 Graptemys spp. Graptemys spp. Map Turtles 

8847013303 553315 Gua. guavina Guavina guavina 

8792012301 164964 Gun. longipenis Gunterichthys longipenis Gold brotula 

8792012300 164963 Gunterichthys spp. Gunterichthys spp. 

8858030301 172991 Gym. melas Gymnachirus melas Naked sole 

8858030300 172990 Gymnachirus spp. Gymnachirus spp. 

8858030303 172993 Gym. texae Gymnachirus texae Fringed sole 

8741050401 161186 Gym. funebris Gymnothorax funebris Green moray 

8741050430 161218 Gym. hubbsi Gymnothorax hubbsi Lichen moray 

8741050446 161235 Gym. kolpos Gymnothorax kolpos 

8741050436 161224 Gym. maderensis Gymnothorax maderensis 

8741050448 161237 Gym. miliaris Gymnothorax miliaris Goldentail moray 

8741050403 161188 Gym. moringa Gymnothorax moringa Spotted moray 

8741050404 161189 Gym. nigromarginatus Gymnothorax nigromarginatus Blackedge moray 

8741050406 161191 Gym. polygonius Gymnothorax polygonius 

8741050407 161192 Gym. saxicola Gymnothorax saxicola Ocellated moray 

8741050400 161185 Gymnothorax spp. Gymnothorax spp. 

8741050408 161193 Gym. vicinus Gymnothorax vicinus Purplemouth moray 

8713050201 160961 Gym. altavela Gymnura altavela Spiny butterfly ray 

8713050202 160962 Gym. micrura Gymnura micrura Smooth butterfly ray 

8713050200 160960 Gymnura spp. Gymnura spp. 

8835400000 169055 Haemulidae spp. Haemulidae spp. 

8835400103 169060 Hae. album Haemulon album Margate 

8835400101 169058 Hae. aurolineatum Haemulon aurolineatum Tomtate 

8835400106 169063 Hae. carbonarium Haemulon carbonarium Caesar grunt 

8835400107 169064 Hae. chrysargyreum Haemulon chrysargyreum Smallmouth grunt 

8835400108 169065 Hae. flavolineatum Haemulon flavolineatum French grunt 

8835400110 169066 Hae. macrostomum Haemulon macrostomum Spanish grunt 

8835400111 169067 Hae. melanurum Haemulon melanurum Cottonwick 

8835400117 169074 Hae. parra Haemulon parra Sailors choice 

8835400102 613026 Hae. plumierii Haemulon plumierii White grunt 

8835400113 169069 Hae. sciurus Haemulon sciurus Bluestriped grunt 

8835400100 169057 Haemulon spp. Haemulon spp. 

8835400116 169073 Hae. striatum Haemulon striatum Striped grunt 

8820020501 166524 Hal. crinitus Halicampus crinitus Banded pipefish 

8820020500 166523 Halicampus spp. Halicampus spp. 

8839010701 170502 Hal. bathyphilus Halichoeres bathyphilus 

8839010702 170503 Hal. bivittatus Halichoeres bivittatus Slippery dick 

8839010703 170504 Hal. caudalis Halichoeres caudalis Painted wrasse 

8839010704 170505 Hal. cyanocephalus Halichoeres cyanocephalus 

8839010705 170506 Hal. garnoti Halichoeres garnoti Yellowhead wrasse 

8839010706 170507 Hal. maculipinna Halichoeres maculipinna Clown wrasse


8839010707 170508 Hal. pictus Halichoeres pictus Rainbow wrasse 

8839010708 170509 Hal. poeyi Halichoeres poeyi Blackear wrasse 

8839010709 170510 Hal. radiatus Halichoeres radiatus Puddingwife 

8839010700 170501 Halichoeres spp. Halichoeres spp. 

8787040301 164594 Hal. aculeatus Halieutichthys aculeatus Pancake batfish 

8787040300 164593 Halieutichthys spp. Halieutichthys spp. 

8747010801 161753 Har. clupeola Harengula clupeola 

8747010802 161754 Har. humeralis Harengula humeralis Redear sardine 

8747010803 161755 Har. jaguana Harengula jaguana Scaled sardine 

8747010800 161752 Harengula spp. Harengula spp. 

8826010301 166787 Hel. dactylopterus Helicolenus dactylopterus 

8835021204 167803 Hem. aureorubens Hemanthias aureorubens 

8835281401 168740 Hem. amblyrhynchus Hemicaranx amblyrhynchus Bluntnose jack 

8835281400 168739 Hemicaranx spp. Hemicaranx spp. 

8835610301 169806 Hem. letourneuxi Hemichromis letourneuxi Jewelfish 

8835610300 169805 Hemichromis spp. Hemichromis spp. 

8842091201 171474 Hem. simula Hemiemblemaria simula Wrasse blenny 

8842091200 171473 Hemiemblemaria spp. Hemiemblemaria spp. 

8803010202 165461 Hem. balao Hemiramphus balao Balao 

8803010201 165460 Hem. brasiliensis Hemiramphus brasiliensis Ballyhoo 

8803010200 165459 Hemiramphus spp. Hemiramphus spp. 

8831021503 167289 Hem. americanus Hemitripterus americanus Sea raven 

8705020301 159844 Hep. perlo Heptranchias perlo 

8835611601 169895 Her. severus Heros severus 

8804080401 165915 Het. formosa Heterandria formosa Least killifish 

8804080400 165914 Heterandria spp. Heterandria spp. 

8741121401 621140 Het. longissimus Heteroconger longissimus 

8741121203 161398 Het. luteolus Heteroconger luteolus 

8835170102 168179 Het. cruentatus Heteropriacanthus cruentatus Glasseye snapper 

8705020101 159819 Hex. griseus Hexanchus griseus 

8705020103 564645 Hex. nakamurai Hexanchus nakamurai 

8788030000 164620 Himantolophidae spp. Himantolophidae spp. 

8788030201 164624 Him. groenlandicus Himantolophus groenlandicus Atlantic footballfish 

8788030200 164623 Himantolophus spp. Himantolophus spp. 

8820020201 166488 Hip. erectus Hippocampus erectus Lined seahorse 

8820020204 166492 Hip. reidi Hippocampus reidi Longsnout seahorse 

8820020200 166487 Hippocampus spp. Hippocampus spp. 

8820020205 166493 Hip. zosterae Hippocampus zosterae Dwarf seahorse 

6179160100 96747 Hippolyte spp. Hippolyte spp. 

6179160104 96751 Hip. zostericola Hippolyte zostericola Zostera shrimp 

6179160000 96746 Hippolytidae spp. Hippolytidae spp. 

8803010901 165516 Hir. affinis Hirundichthys affinis Fourwing flyingfish


8803010903 616693 Hir. rondeletii Hirundichthys rondeletii Blackwing flyingfish 

8803010900 165515 Hirundichthys spp. Hirundichthys spp. 

8787020101 164520 His. histrio Histrio histrio Sargassumfish 

8787020100 164519 Histrio spp. Histrio spp. 

8835550304 169626 Hol. bermudensis Holacanthus bermudensis Blue angelfish 

8835550301 169623 Hol. ciliaris Holacanthus ciliaris Queen angelfish 

8835550300 169622 Holacanthus spp. Holacanthus spp. 

8835550303 169625 Hol. tricolor Holacanthus tricolor Rock beauty 

8860010101 173116 Hol. hollardi Hollardia hollardi 

8860010102 173117 Hol. meadi Hollardia meadi Spotted spikefish 

8860010100 173115 Hollardia spp. Hollardia spp. 

8810080000 166170 Holocentridae spp. Holocentridae spp. 

8810080101 166172 Hol. adscensionis Holocentrus adscensionis Squirrelfish 

8810080103 166173 Hol. rufus Holocentrus rufus Longspine squirrelfish 

8810080100 166171 Holocentrus spp. Holocentrus spp. 

8777300301 164351 Hop. littorale Hoplosternum littorale 

8777300300 553264 Hoplosternum spp. Hoplosternum spp. 

8741080101 161290 Hop. diomediana Hoplunnis diomediana 

8741080102 161291 Hop. macrura Hoplunnis macrura 

8741080103 161292 Hop. tenuis Hoplunnis tenuis 

8776010506 163364 Hyb. hayi Hybognathus hayi Cypress minnow 

8776010500 163358 Hybognathus spp. Hybognathus spp. 

8776011200 163495 Hybopsis spp. Hybopsis spp. 

8776011220 201918 Hyb. winchelli Hybopsis winchelli 

8851010202 172513 Hyp. bythites Hyperoglyphe bythites Black driftfish 

8851010201 172512 Hyp. perciformis Hyperoglyphe perciformis Barrelfish 

8851010200 172511 Hyperoglyphe spp. Hyperoglyphe spp. 

8842010599 171205 Hyp. multifilis Hypeurochilus multifilis Plumed blenny 

8842010503 171200 Hyp. bermudensis Hypleurochilus bermudensis Barred blenny 

8842010598 171204 Hyp. caudovittatus Hypleurochilus caudovittatus 

8842010501 171198 Hyp. geminatus Hypleurochilus geminatus Crested blenny 

8842010502 171199 Hyp. pseudoaequipinnis 

Hypleurochilus 

pseudoaequipinnis 

Oyster blenny 

8842010500 171197 Hypleurochilus spp. Hypleurochilus spp. 

8842010504 171201 Hyp. springeri Hypleurochilus springeri Orangespotted blenny 

8805021201 166038 Hyp. harringtonensis Hypoatherina harringtonensis Reef silverside 

8805021200 166037 Hypoatherina spp. Hypoatherina spp. 

8835021302 167807 Hyp. aberrans Hypoplectrus aberrans 

8835021303 167808 Hyp. chlorurus Hypoplectrus chlorurus 

8835021304 167809 Hyp. gemma Hypoplectrus gemma 

8835021305 167810 Hyp. guttavarius Hypoplectrus guttavarius 

8835021306 167811 Hyp. indigo Hypoplectrus indigo


8835021307 167812 Hyp. nigricans Hypoplectrus nigricans 

8835021308 167813 Hyp. puella Hypoplectrus puella 

8835021300 167805 Hypoplectrus spp. Hypoplectrus spp. 

8835021301 167806 Hyp. unicolor Hypoplectrus unicolor Butter hamlet 

8803010399 616683 Hyp. meeki Hyporhamphus meeki 

8803010300 165473 Hyporhamphus spp. Hyporhamphus spp. 

8803010301 165474 Hyp. unifasciatus Hyporhamphus unifasciatus Halfbeak 

8777300101 164342 Hyp. plecostomus Hypostomus plecostomus Suckermouth catfish 

8777300100 164341 Hypostomus spp. Hypostomus spp. 

8842010201 171156 Hyp. hentz Hypsoblennius hentz Feather blenny 

8842010202 171157 Hyp. ionthas Hypsoblennius ionthas Freckled blenny 

8842010200 171155 Hypsoblennius spp. Hypsoblennius spp. 

8741132502 161529 Ich. ophioneus Ichthyapus ophioneus 

8603010404 159727 Ich. gagei Ichthyomyzon gagei 

8777020000 163995 Ictaluridae spp. Ictaluridae spp. 

8777020102 163997 Ict. furcatus Ictalurus furcatus Blue catfish 

8777020105 163998 Ict. punctatus Ictalurus punctatus Channel catfish 

8777020100 163996 Ictalurus spp. Ictalurus spp. 

8835350200 168831 Inermia spp. Inermia spp. 

8835350201 168832 Ine. vittata Inermia vittata Boga 

8835350000 168828 Inermiidae spp. Inermiidae spp. 

8768010000 162827 Ipnopidae spp. Ipnopidae spp. 

8710010601 160683 Isi. brasiliensis Isistius brasiliensis 

8850060000 172486 Istiophoridae spp. Istiophoridae spp. 

8850060101 172488 Ist. platypterus Istiophorus platypterus Sailfish 

8850060100 172487 Istiophorus spp. Istiophorus spp. 

8707040501 159924 Isu. oxyrinchus Isurus oxyrinchus Shortfin mako 

8707040502 159926 Isu. paucus Isurus paucus 

8707040500 159923 Isurus spp. Isurus spp. Mako 

8747010901 161759 Jen. lamprotaenia Jenkinsia lamprotaenia Dwarf herring 

8747010902 161760 Jen. majua Jenkinsia majua Little-eye herring 

8747010900 161758 Jenkinsia spp. Jenkinsia spp. 

8747010903 161761 Jen. stolifera Jenkinsia stolifera Shortband herring 

8804040801 165694 Jor. floridae Jordanella floridae Flagfish 

8804040800 165693 Jordanella spp. Jordanella spp. 

8840140301 171061 Kat. albigutta Kathetostoma albigutta Lancer stargazer 

8840140300 171060 Kathetostoma spp. Kathetostoma spp. 

8850030101 172401 Kat. pelamis Katsuwonus pelamis Skipjack tuna 

8741040201 161152 Kau. hyoproroides Kaupichthys hyoproroides False moray 

8741040202 161153 Kau. nuchalis Kaupichthys nuchalis Collared eel 

8741040200 161151 Kaupichthys spp. Kaupichthys spp. 

9002020000 173756 Kinosternidae spp. Kinosternidae spp. Musk turtles


9002020202 173765 Kin. baurii Kinosternon baurii Striped mud turtle 

9002020200 173762 Kinosternon spp. Kinosternon spp. Mud turtles 

900202020103 208573 Kin. sub. steindachneri 

Kinosternon subrubrum 

steindachneri 

Florida mud turtle 

8835510000 169503 Kyphosidae spp. Kyphosidae spp. 

8835510101 169505 Kyp. incisor Kyphosus incisor Yellow chub 

8835510102 169506 Kyp. sectatrix Kyphosus sectatrix Bermuda chub 

8835510100 169504 Kyphosus spp. Kyphosus spp. 

8805020801 166016 Lab. sicculus Labidesthes sicculus Brook silverside 

8805020800 166015 Labidesthes spp. Labidesthes spp. 

8839010000 170477 Labridae spp. Labridae spp. 

8842090000 171396 Labrisomidae spp. Labrisomidae spp. 

8842090301 171409 Lab. bucciferus Labrisomus bucciferus Puffcheek blenny 

8842090303 171411 Lab. gobio Labrisomus gobio Palehead blenny 

8842090304 171412 Lab. guppyi Labrisomus guppyi Mimic blenny 

8842090305 171413 Lab. haitiensis Labrisomus haitiensis Longfin blenny 

8842090308 171416 Lab. kalisherae Labrisomus kalisherae Downy blenny 

8842090306 171414 Lab. nigricinctus Labrisomus nigricinctus Spotcheek blenny 

8842090307 171415 Lab. nuchipinnis Labrisomus nuchipinnis Hairy blenny 

8842090300 171408 Labrisomus spp. Labrisomus spp. 

8839010901 170566 Lac. maximus Lachnolaimus maximus Hogfish 

8860030102 173238 Lac. bicaudalis Lactophrys bicaudalis Spotted trunkfish 

8860030100 173236 Lactophrys spp. Lactophrys spp. 

8860030101 173237 Lac. trigonus Lactophrys trigonus Trunkfish 

8860030103 173239 Lac. triqueter Lactophrys triqueter Smooth trunkfish 

8791010201 164675 Lae. barbatulum Laemonema barbatulum 

8861010101 173285 Lag. laevigatus Lagocephalus laevigatus Smooth puffer 

8861010100 173284 Lagocephalus spp. Lagocephalus spp. 

8835430201 169187 Lag. rhomboides Lagodon rhomboides Pinfish 

8835430200 169186 Lagodon spp. Lagodon spp. 

8707040000 159901 Lamnidae spp. Lamnidae spp. 

8813010000 615903 Lamprididae spp. Lamprididae spp. 

8813010102 166326 Lam. guttatus Lampris guttatus Opah 

8813010100 166324 Lampris spp. Lampris spp. 

8835440501 169269 Lar. fasciatus Larimus fasciatus Banded drum 

8835440500 169268 Larimus spp. Larimus spp. 

6179160602 96871 Lat. parvulus Latreutes parvulus 

6179160600 96869 Latreutes spp. Latreutes spp. 

6179110105 96219 Lea. paulensis Leander paulensis 

6179110100 96214 Leander spp. Leander spp. 

6179110101 96215 Lea. tenuicornis Leander tenuicornis Brown grass shrimp 

8835440400 169266 Leiostomus spp. Leiostomus spp.


8835440401 169267 Lei. xanthurus Leiostomus xanthurus Spot 

9002040401 551770 Lep. kempii Lepidochelys kempii Kemps Ridley sea turtle 

9002040402 173840 Lep. olivacea Lepidochelys olivacea Olive Ridley sea turtle 

9002040400 173838 Lepidochelys spp. Lepidochelys spp. Ridley sea turtles 

8850010301 172362 Lep. flavobrunneum Lepidocybium flavobrunneum 

8732010000 161092 Lepisosteidae spp. Lepisosteidae spp. 

8732010102 161095 Lep. oculatus Lepisosteus oculatus Spotted gar 

8732010101 161094 Lep. osseus Lepisosteus osseus Longnose gar 

8732010105 161098 Lep. platyrhincus Lepisosteus platyrhincus Florida gar 

8732010100 161093 Lepisosteus spp. Lepisosteus spp. 

8835160501 168131 Lep. auritus Lepomis auritus Redbreast sunfish 

8835160502 168132 Lep. cyanellus Lepomis cyanellus Green sunfish 

8835161001 168138 Lep. gulosus Lepomis gulosus Warmouth 

8835160506 168151 Lep. humilis Lepomis humilis 

8835160504 168141 Lep. macrochirus Lepomis macrochirus Bluegill 

8835160507 168152 Lep. marginatus Lepomis marginatus Dollar sunfish 

8835160508 168153 Lep. megalotis Lepomis megalotis Longear sunfish 

8835160509 168154 Lep. microlophus Lepomis microlophus Redear sunfish 

8835160510 168155 Lep. punctatus Lepomis punctatus Spotted sunfish 

8835160500 168130 Lepomis spp. Lepomis spp. 

8792010502 164825 Lep. brevibarbe Lepophidium brevibarbe 

8792010505 164827 Lep. jeannae Lepophidium jeannae 

8792010509 164831 Lep. profundorum Lepophidium profundorum 

8792010500 164823 Lepophidium spp. Lepophidium spp. 

8792010510 164833 Lep. staurophor Lepophidium staurophor 

8804040901 165696 Lep. ommata Leptolucania ommata Pygmy killifish 

8804040900 165695 Leptolucania spp. Leptolucania spp. 

8741130600 161440 Letharchus spp. Letharchus spp. 

8741130601 161441 Let. velifer Letharchus velifer Sailfin eel 

8713040124 564136 Leu. garmani Leucoraja garmani Rosette skate 

8713040126 564140 Leu. lentiginosa Leucoraja lentiginosa 

5802010101 82703 Lim. polyphemus Limulus polyphemus Horseshoe crab 

8835021408 167827 Lio. carmabi Liopropoma carmabi 

8835021402 167818 Lio. eukrines Liopropoma eukrines 

8835021403 167819 Lio. mowbrayi Liopropoma mowbrayi 

8835021404 167820 Lio. rubre Liopropoma rubre 

8835060102 168015 Lip. anabantoides Lipogramma anabantoides 

8835060100 168013 Lipogramma spp. Lipogramma spp. 

8835060101 168014 Lip. trilineatum Lipogramma trilineatum Threeline basslet 

6177010103 551680 Lit. setiferus Litopenaeus setiferus White shrimp 

8835380100 169006 Lobotes spp. Lobotes spp. 

8835380101 169007 Lob. surinamensis Lobotes surinamensis Tripletail


8835380000 169005 Lobotidae spp. Lobotidae spp. 

5706010000 82369 Loliginidae spp. Loliginidae spp. Squid 

5706010100 82370 Loligo spp. Loligo spp. Squid 

8840020103 170924 Lon. micrognathus Lonchopisthus micrognathus Swordtail jawfish 

8840020100 170921 Lonchopisthus spp. Lonchopisthus spp. 

8786010202 164505 Lop. reticulatus Lophiodes reticulatus 

8786010101 164499 Lop. americanus Lophius americanus 

8847012501 171896 Lop. cyprinoides Lophogobius cyprinoides Crested goby 

8847012500 171895 Lophogobius spp. Lophogobius spp. 

8835220201 168546 Lop. chamaeleonticeps Lopholatilus chamaeleonticeps Tilefish 

8835220200 168545 Lopholatilus spp. Lopholatilus spp. Tilefish 

8815040101 621143 Lop. lacepede Lophotus lacepede 

8777300000 164340 Loricariidae spp. Loricariidae spp. Suckermouth catfish 

8804040302 165680 Luc. goodei Lucania goodei Bluefin killifish 

8804040301 165679 Luc. parva Lucania parva Rainwater killifish 

8804040300 165678 Lucania spp. Lucania spp. 

8792011701 164938 Luc. corethromycter Luciobrotula corethromycter 

8792011700 164937 Luciobrotula spp. Luciobrotula spp. 

8842011402 171256 Lup. nicholsi Lupinoblennius nicholsi Highfin blenny 

8842011400 171254 Lupinoblennius spp. Lupinoblennius spp. 

8842011401 171255 Lup. vinctus Lupinoblennius vinctus Mangrove blenny 

8835360000 168845 Lutjanidae spp. Lutjanidae spp. 

8835360103 168849 Lut. analis Lutjanus analis Mutton snapper 

8835360104 168850 Lut. apodus Lutjanus apodus Schoolmaster 

8835360106 168852 Lut. buccanella Lutjanus buccanella Blackfin snapper 

8835360107 168853 Lut. campechanus Lutjanus campechanus Red snapper 

8835360101 168847 Lut. cyanopterus Lutjanus cyanopterus Cubera snapper 

8835360102 168848 Lut. griseus Lutjanus griseus Gray snapper 

8835360109 168857 Lut. jocu Lutjanus jocu Dog snapper 

8835360110 168858 Lut. mahogoni Lutjanus mahogoni Mahogany snapper 

8835360111 168859 Lut. purpureus Lutjanus purpureus 

8835360100 168846 Lutjanus spp. Lutjanus spp. 

8835360112 168860 Lut. synagris Lutjanus synagris Lane snapper 

8835360113 168861 Lut. vivanus Lutjanus vivanus Silk snapper 

8850050000 172483 Luvaridae spp. Luvaridae spp. 

8850050101 172485 Luv. imperialis Luvarus imperialis Louvar 

8850050100 172484 Luvarus spp. Luvarus spp. 

8776017804 163832 Lux. chrysocephalus Luxilus chrysocephalus Striped shiner 

8776017809 163843 Lux. zonistius Luxilus zonistius Bandfin shiner 

6179161109 621164 Lys. amboinensis Lysmata amboinensis 

6179161103 96895 Lys. rathbunae Lysmata rathbunae 

6179161100 96892 Lysmata spp. Lysmata spp.


6179161101 96893 Lys. wurdemanni Lysmata wurdemanni Peppermint shrimp 

8776017902 163849 Lyt. atrapiculus Lythrurus atrapiculus Blacktip shiner 

8847012606 171904 Lyt. elasson Lythrypnus elasson 

8847012602 171900 Lyt. nesiotes Lythrypnus nesiotes Island goby 

8847012603 171901 Lyt. phorellus Lythrypnus phorellus Convict goby 

8847012604 171902 Lyt. spilus Lythrypnus spilus Bluegold goby 

8847012600 171898 Lythrypnus spp. Lythrypnus spp. 

8743030102 161688 Mac. rostrata Macdonaldi rostrata 

8776018000 163863 Macrhybopsis spp. Macrhybopsis spp. Florida chub 

6179110201 96221 Mac. ohione Macrobrachium ohione Ohio shrimp 

6179110200 96220 Macrobrachium spp. Macrobrachium spp. Prawns 

9002010200 173754 Macrochelys spp. Macrochelys spp. alligator snapping turtles 

9002010201 173755 Mac. temminckii Macrochelys temminckii alligator snapping turtle 

8854010102 621146 Mac. siamensis Macrognathus siamensis 

8854010100 172688 Macrognathus spp. Macrognathus spp. 

8852020401 172642 Mac. opercularis Macropodus opercularis 

8852020400 172641 Macropodus spp. Macropodus spp. 

8819030102 551496 Mac. gracilis Macroramphosus gracilis Slender snipefish 

8819030101 551497 Mac. scolopax Macroramphosus scolopax Longspine snipefish 

8819030100 166422 Macroramphosus spp. Macroramphosus spp. 

8794010000 165332 Macrouridae spp. Macrouridae spp. 

8850060201 172491 Mak. nigricans Makaira nigricans Blue marlin 

8850060200 172490 Makaira spp. Makaira spp. 

8835220000 168537 Malacanthidae spp. Malacanthidae spp. 

8835220301 168548 Mal. plumieri Malacanthus plumieri Sand tilefish 

8835220300 168547 Malacanthus spp. Malacanthus spp. 

9002030300 173779 Malaclemys spp. Malaclemys spp. Terrapin 

9002030301 173780 Mal. terrapin Malaclemys terrapin Diamondback terrapin 

8794010601 165389 Mal. laevis Malacocephalus laevis 

8794010602 165390 Mal. occidentalis Malacocephalus occidentalis 

8794010600 165388 Malacocephalus spp. Malacocephalus spp. 

8842090401 171420 Mal. aurolineatus Malacoctenus aurolineatus Goldline blenny 

8842090403 171422 Mal. macropus Malacoctenus macropus Rosy blenny 

8842090400 171419 Malacoctenus spp. Malacoctenus spp. 

8842090404 171423 Mal. triangulatus Malacoctenus triangulatus Saddled blenny 

8787040500 164599 Malthopsis spp. Malthopsis spp. 

8713080101 160992 Man. birostris Manta birostris Atlantic manta 

8713080100 160991 Manta spp. Manta spp. 

8854010000 172687 Mastacembelidae spp. Mastacembelidae spp. 

8738020000 161114 Megalopidae spp. Megalopidae spp. 

8738020201 161116 Meg. atlanticus Megalops atlanticus Tarpon 

8738020200 161115 Megalops spp. Megalops spp.


8860020601 173174 Mel. niger Melichthys niger Black durgon 

8860020600 173173 Melichthys spp. Melichthys spp. 

8805020201 165989 Mem. martinica Membras martinica Rough silverside 

8805020200 165988 Membras spp. Membras spp. 

8805020301 165993 Men. beryllina Menidia beryllina Inland silverside 

8805020303 165995 Men. conchorum Menidia conchorum Key silverside 

8805020302 165994 Men. menidia Menidia menidia Atlantic silverside 

8805020304 165996 Men. peninsulae Menidia peninsulae Tidewater silverside 

8805020300 165992 Menidia spp. Menidia spp. 

6189021302 98812 Men. adina Menippe adina 

6189021301 98811 Men. mercenaria Menippe mercenaria 

6189021303 98813 Men. nodifrons Menippe nodifrons 

6189021300 98810 Menippe spp. Menippe spp. 

8835440601 169274 Men. americanus Menticirrhus americanus Southern kingfish 

8835440602 169275 Men. littoralis Menticirrhus littoralis Gulf kingfish 

8835440603 169276 Men. saxatilis Menticirrhus saxatilis Northern kingfish 

8835440600 169273 Menticirrhus spp. Menticirrhus spp. 

5515471102 81499 Mer. campechiensis Mercenaria campechiensis 

5515471101 81496 Mer. mercenaria Mercenaria mercenaria 

5515471100 81495 Mercenaria spp. Mercenaria spp. 

8791040101 164791 Mer. bilinearis Merluccius bilinearis 

8847060000 172161 Microdesmidae spp. Microdesmidae spp. 

8847060102 172164 Mic. lanceolatus Microdesmus lanceolatus Lancetail wormfish 

8847060103 172165 Mic. longipinnis Microdesmus longipinnis Pink wormfish 

8847060100 172163 Microdesmus spp. Microdesmus spp. 

8847010703 171810 Mic. carri Microgobius carri Seminole goby 

8847010701 171808 Mic. gulosus Microgobius gulosus Clown goby 

8847010704 171811 Mic. microlepis Microgobius microlepis Banner goby 

8847010700 171807 Microgobius spp. Microgobius spp. 

8847010702 171809 Mic. thalassinus Microgobius thalassinus Green goby 

8820020601 166533 Mic. brachyurus Microphis brachyurus Opposum pipefish 

8820020600 166532 Microphis spp. Microphis spp. 

8835440700 169282 Micropogonias spp. Micropogonias spp. 

8835440701 169283 Mic. undulatus Micropogonias undulatus Atlantic croaker 

8835160607 564610 Mic. cataractae Micropterus cataractae 

8835160606 168164 Mic. notius Micropterus notius 

8835160603 168161 Mic. punctulatus Micropterus punctulatus Spotted bass 

8835160602 168160 Mic. salmoides Micropterus salmoides Largemouth bass 

8835160600 168158 Micropterus spp. Micropterus spp. 

8835620401 170116 Mic. chrysurus Microspathodon chrysurus Yellowtail damselfish 

8835620400 170115 Microspathodon spp. Microspathodon spp. 

8776040801 163959 Min. melanops Minytrema melanops Spotted sucker


8776040800 163958 Minytrema spp. Minytrema spp. Suckers 

8776060101 163978 Mis. anguillicaudatus Misgurnus anguillicaudatus Weatherfish 

8776060100 163977 Misgurnus spp. Misgurnus spp. 

8713080202 160997 Mob. hypostoma Mobula hypostoma Devil ray 

8713080200 160995 Mobula spp. Mobula spp. 

8713080000 160990 Mobulidae spp. Mobulidae spp. 

8861040102 621163 Mol. lanceolata Mola lanceolata 

8861040101 173414 Mol. mola Mola mola Ocean sunfish 

8861040100 173413 Mola spp. Mola spp. Ocean sunfish 

8860040000 203428 Monacanthidae spp. Monacanthidae spp. 

8860020701 173179 Mon. ciliatus Monacanthus ciliatus Fringed filefish 

8860020700 173178 Monacanthus spp. Monacanthus spp. 

8860020702 173180 Mon. tuckeri Monacanthus tuckeri Slender filefish 

8857031201 172786 Mon. antillarum Monolene antillarum 

8857031204 172789 Mon. sessilicauda Monolene sessilicauda 

8824010201 166697 Mon. albus Monopterus albus 

8741020101 161138 Mor. edwardsi Moringua edwardsi 

8835750204 167682 Mor. chrysops Morone chrysops White bass 

8835750203 167681 Mor. chrysops x saxatilis Morone chrysops x saxatilis 

8835750205 167683 Mor. mississippiensis Morone mississippiensis Yellow bass 

8835750202 167680 Mor. saxatilis Morone saxatilis Striped bass 

8835750200 167676 Morone spp. Morone spp. 

8776040407 163936 Mox. carinatum Moxostoma carinatum River redhorse 

8776040403 163932 Mox. poecilurum Moxostoma poecilurum Blacktail redhorse 

8776040400 163927 Moxostoma spp. Moxostoma spp. 

8836010101 170335 Mug. cephalus Mugil cephalus Striped mullet 

8836010102 170336 Mug. curema Mugil curema White mullet 

8836010117 170351 Mug. gyrans Mugil gyrans Fantail mullet 

8836010104 170338 Mug. liza Mugil liza Liza 

8836010103 170337 Mug. sp. (redeye mullet) Mugil sp. (redeye mullet) Redeye mullet 

8836010100 170334 Mugil spp. Mugil spp. 

8836010105 170339 Mug. trichodon Mugil trichodon 

8836010000 170333 Mugilidae spp. Mugilidae spp. 

8835450000 169406 Mullidae spp. Mullidae spp. 

8835450101 169408 Mul. martinicus Mulloidichthys martinicus Yellow goatfish 

8835450100 169407 Mulloidichthys spp. Mulloidichthys spp. 

8835450201 169417 Mul. auratus Mullus auratus Red goatfish 

8835450200 169416 Mullus spp. Mullus spp. 

8741050502 161240 Mur. retifera Muraena retifera Reticulate moray 

8741050504 161242 Mur. robusta Muraena robusta 

8741050500 161238 Muraena spp. Muraena spp. 

8741050000 161160 Muraenidae spp. Muraenidae spp.


8708040201 160230 Mus. canis Mustelus canis Smooth dogfish 

8708040203 160234 Mus. norrisi Mustelus norrisi Florida smoothhound 

8708040222 621134 Mus. sinusmexicanus Mustelus sinusmexicanus 

8708040200 160226 Mustelus spp. Mustelus spp. 

8835020502 167760 Myc. bonaci Mycteroperca bonaci Black grouper 

8835020504 167762 Myc. interstitialis Mycteroperca interstitialis Yellowmouth grouper 

8835020501 167759 Myc. microlepis Mycteroperca microlepis Gag 

8835020505 167763 Myc. phenax Mycteroperca phenax Scamp 

8835020500 167758 Mycteroperca spp. Mycteroperca spp. 

8835020509 167767 Myc. tigris Mycteroperca tigris Tiger grouper 

8835020506 167764 Myc. venenosa Mycteroperca venenosa Yellowfin grouper 

8713070000 160976 Myliobatidae spp. Myliobatidae spp. 

8713070201 564391 Myl. freminvillii Myliobatis freminvillii Bullnose ray 

8713070203 160982 Myl. goodei Myliobatis goodei Southern eagle ray 

8713070200 160979 Myliobatis spp. Myliobatis spp. 

8741130707 161449 Myr. breviceps Myrichthys breviceps 

8741130708 161450 Myr. ocellatus Myrichthys ocellatus 

8810080201 166211 Myr. jacobus Myripristis jacobus 

8741130802 161453 Myr. punctatus Myrophis punctatus Speckled worm eel 

8741130800 161451 Myrophis spp. Myrophis spp. 

8713120101 160844 Nar. bancroftii Narcine bancroftii Lesser electric ray 

8713120100 160843 Narcine spp. Narcine spp. 

8835281501 168742 Nau. ductor Naucrates ductor 

8708020801 160433 Neg. brevirostris Negaprion brevirostris Lemon shark 

8708020800 160432 Negaprion spp. Negaprion spp. 

8842091501 171482 Nem. atelestos Nemaclinus atelestos Threadfin blenny 

8842091500 171481 Nemaclinus spp. Nemaclinus spp. 

8741020201 161143 Neo. mucronatus Neoconger mucronatus 

8837020101 550874 Neo. americana Neoepinnula americana 

8826010402 166794 Neo. hemingwayi Neomerinthe hemingwayi 

8810080105 166175 Neo. marianus Neoniphon marianus Longjaw squirrelfish 

8847012701 171908 Nes. longus Nes longus 

8850010701 172370 Nes. nasutus Nesiarchus nasutus 

8741080201 621141 Net. pygmaea Nettenchelys pygmaea 

8794010802 165395 Nez. bairdii Nezumia bairdii 

8839030300 170859 Nicholsina spp. Nicholsina spp. 

8839030301 170860 Nic. usta Nicholsina usta Emerald parrotfish 

9998000000 9998 No fish No fish 

0000000000 0 No gear set No gear set 

8776011002 163393 Noc. leptocephalus Nocomis leptocephalus Bluehead chub 

8776011000 163391 Nocomis spp. Nocomis spp. 

8851020000 172535 Nomeidae spp. Nomeidae spp.


8851020301 172554 Nom. gronovii Nomeus gronovii Man-of-war fish 

8851020300 172553 Nomeus spp. Nomeus spp. 

1111000000 1111 Non-standard work up Non-standard work up 

8743030301 161690 Not. chemnitzii Notacanthus chemnitzii 

8776010601 163368 Not. crysoleucas Notemigonus crysoleucas Golden shiner 

8776010600 163367 Notemigonus spp. Notemigonus spp. 

8776011137 163427 Not. baileyi Notropis baileyi Rough shiner 

8776015012 163479 Not. buccatus Notropis buccatus Silverjaw minnow 

8776011104 163403 Not. chalybaeus Notropis chalybaeus Ironcolor shiner 

8776011157 163438 Not. cummingsae Notropis cummingsae Dusky shiner 

8776011166 163444 Not. harperi Notropis harperi Redeye chub 

8776011176 163452 Not. longirostris Notropis longirostris Longnose shiner 

8776011178 163454 Not. maculatus Notropis maculatus Taillight shiner 

8776015016 163485 Not. melanostomus Notropis melanostomus 

8776011185 163460 Not. petersoni Notropis petersoni Coastal shiner 

8776011100 163399 Notropis spp. Notropis spp. 

8776011124 163420 Not. texanus Notropis texanus Weed shiner 

8777020212 164014 Not. funebris Noturus funebris Black madtom 

8777020201 164003 Not. gyrinus Noturus gyrinus Tadpole madtom 

8777020217 164019 Not. leptacanthus Noturus leptacanthus Speckled madtom 

8777020200 164002 Noturus spp. Noturus spp. 

8835360401 168907 Ocy. chrysurus Ocyurus chrysurus Yellowtail snapper 

8835360400 168906 Ocyurus spp. Ocyurus spp. 

8707030000 159875 Odontaspididae spp. Odontaspididae spp. 

8835441301 169325 Odo. dentex Odontoscion dentex Reef croaker 

8835441300 169324 Odontoscion spp. Odontoscion spp. 

8787040000 164573 Ogcocephalidae spp. Ogcocephalidae spp. 

8787040109 164584 Ogc. corniger Ogcocephalus corniger 

8787040113 164581 Ogc. cubifrons Ogcocephalus cubifrons 

8787040108 164583 Ogc. declivirostris Ogcocephalus declivirostris 

8787040103 164576 Ogc. nasutus Ogcocephalus nasutus Shortnose batfish 

8787040106 164580 Ogc. pantostictus Ogcocephalus pantostictus 

8787040105 164578 Ogc. parvus Ogcocephalus parvus Roughback batfish 

8787040110 164585 Ogc. rostellum Ogcocephalus rostellum 

8787040100 164574 Ogcocephalus spp. Ogcocephalus spp. 

8792012401 164966 Ogi. cayorum Ogilbia cayorum Key brotula 

8792012400 164965 Ogilbia spp. Ogilbia spp. 

8835280501 168673 Oli. saurus Oligoplites saurus Leatherjacket 

8835280500 168672 Oligoplites spp. Oligoplites spp. 

8741130000 161419 Ophichthidae spp. Ophichthidae spp. 

8741131010 161473 Oph. cruentifer Ophichthus cruentifer 

8741131001 161462 Oph. gomesii Ophichthus gomesii Shrimp eel


8741131013 161479 Oph. hyposagmatus Ophichthus hyposagmatus 

8741131002 161463 Oph. melanoporus Ophichthus melanoporus Blackpored eel 

8741131016 621136 Oph. menezesi Ophichthus menezesi 

8741131014 161480 Oph. omorgmus Ophichthus omorgmus 

8741131004 161465 Oph. ophis Ophichthus ophis Spotted snake eel 

8741131015 161481 Oph. puncticeps Ophichthus puncticeps Palespotted eel 

8741131009 161472 Oph. rex Ophichthus rex 

8741131000 161458 Ophichthus spp. Ophichthus spp. 

8792010000 164807 Ophidiidae spp. Ophidiidae spp. 

8792010601 164840 Oph. antipholus Ophidion antipholus Long nosed cusk ell 

8792010619 621144 Oph. dromio Ophidion dromio 

8792010602 164841 Oph. grayi Ophidion grayi Blotched cusk-eel 

8792010603 164842 Oph. holbrookii Ophidion holbrookii Bank cusk-eel 

8792010605 164844 Oph. josephi Ophidion josephi Crested cusk-eel 

8792010613 164852 Oph. marginatum Ophidion marginatum Striped cusk-eel 

8792010604 164843 Oph. selenops Ophidion selenops 

8792010600 164839 Ophidion spp. Ophidion spp. 

8842010601 171203 Oph. macclurei Ophioblennius macclurei Redlip blenny 

8842010600 171202 Ophioblennius spp. Ophioblennius spp. 

8747010701 161748 Opi. oglinum Opisthonema oglinum Atlantic thread herring 

8747010700 161747 Opisthonema spp. Opisthonema spp. 

8840020000 170920 Opistognathidae spp. Opistognathidae spp. 

8840020203 170928 Opi. aurifrons Opistognathus aurifrons Yellowhead jawfish 

8840020201 170926 Opi. lonchurus Opistognathus lonchurus Moustache jawfish 

8840020206 170931 Opi. macrognathus Opistognathus macrognathus Banded jawfish 

8840020202 170927 Opi. maxillosus Opistognathus maxillosus Mottled jawfish 

8840020211 621148 Opi. nothus Opistognathus nothus 

8840020299 170933 Opi. robinsi Opistognathus robinsi Spotfin jawfish 

8840020200 170925 Opistognathus spp. Opistognathus spp. Spotfin jawfishes 

8840020207 170932 Opi. whitehursti Opistognathus whitehursti Dusky jawfish 

8783010202 164424 Ops. beta Opsanus beta Gulf toadfish 

8783010203 164425 Ops. pardus Opsanus pardus Leopard toadfish 

8783010200 164422 Opsanus spp. Opsanus spp. 

8783010201 164423 Ops. tau Opsanus tau Oyster toadfish 

8776018201 163876 Ops. emiliae Opsopoeodus emiliae Pugnose minnow 

8835610401 169810 Ore. aureus Oreochromis aureus Blue tilapia 

8835614001 170015 Ore. mossambicus Oreochromis mossambicus Mozambique tilapia 

8835614003 553310 Ore. niloticus Oreochromis niloticus 

8835400201 169077 Ort. chrysoptera Orthopristis chrysoptera Pigfish 

8835400200 169076 Orthopristis spp. Orthopristis spp. 

8810080300 166239 Ostichthys spp. Ostichthys spp. 

8810080301 166240 Ost. trachypoma Ostichthys trachypoma Bigeye soldierfish


8860030000 173235 Ostraciidae spp. Ostraciidae spp. 

8792010703 164862 Oto. dormitator Otophidium dormitator Sleeper cusk-eel 

8792010701 164859 Oto. omostigma Otophidium omostigma Polka-dot cusk-eel 

8792010700 164858 Otophidium spp. Otophidium spp. 

8803011001 165520 Oxy. micropterus Oxyporhamphus micropterus Smallwing flyingfish 

8803011000 165519 Oxyporhamphus spp. Oxyporhamphus spp. 

8847010505 171773 Oxy. stigmalophius Oxyurichthys stigmalophius Spotfin goby 

8835430601 169207 Pag. pagrus Pagrus pagrus Red porgy 

8835430600 169206 Pagrus spp. Pagrus spp. 

6179110803 96465 Pal. floridanus Palaemon floridanus 

6179110800 96449 Palaemon spp. Palaemon spp. 

6179110301 96384 Pal. intermedius Palaemonetes intermedius 

6179110302 96385 Pal. paludosus Palaemonetes paludosus 

6179110303 96390 Pal. pugio Palaemonetes pugio 

6179110300 96383 Palaemonetes spp. Palaemonetes spp. Grass shrimp 

6179110304 96391 Pal. vulgaris Palaemonetes vulgaris 

6179110000 96213 Palaemonidae spp. Palaemonidae spp. 

8847014801 171969 Pal. paradoxus Palatogobius paradoxus Mauve goby 

8847014800 171968 Palatogobius spp. Palatogobius spp. 

6182010000 97646 Palinuridae spp. Palinuridae spp. 

6182010101 97648 Pan. argus Panulirus argus Spiny lobster 

6182010100 97647 Panulirus spp. Panulirus spp. 

8842012501 171304 Par. marmoreus Parablennius marmoreus Seaweed blenny 

8842012500 171303 Parablennius spp. Parablennius spp. 

8842090502 171429 Par. cingulatus Paraclinus cingulatus Coral blenny 

8842090503 171430 Par. fasciatus Paraclinus fasciatus Banded blenny 

8842090501 171428 Par. grandicomis Paraclinus grandicomis Horned blenny 

8842090504 171431 Par. infrons Paraclinus infrons 

8842090506 171433 Par. marmoratus Paraclinus marmoratus Marbled blenny 

8842090507 171434 Par. nigripinnis Paraclinus nigripinnis Blackfin blenny 

8842090500 171427 Paraclinus spp. Paraclinus spp. 

8741120501 161369 Par. caudilimbatus Paraconger caudilimbatus Margintail conger 

8741120500 161368 Paraconger spp. Paraconger spp. 

8846010901 171743 Par. bairdi Paradiplogrammus bairdi Lancer dragonet 

8860010301 173121 Par. lineata Parahollardia lineata 

8857030302 172736 Par. albigutta Paralichthys albigutta Gulf flounder 

8857030301 172735 Par. dentatus Paralichthys dentatus Summer flounder 

8857030304 172738 Par. lethostigma Paralichthys lethostigma Southern flounder 

8857030305 172739 Par. oblongus Paralichthys oblongus Fourspot flounder 

8857030300 172734 Paralichthys spp. Paralichthys spp. 

8857030306 172740 Par. squamilentus Paralichthys squamilentus Broad flounder 

8835021701 167838 Par. furcifer Paranthias furcifer Creole-fish


8835021700 167837 Paranthias spp. Paranthias spp. 

8762040200 162434 Parasudis spp. Parasudis spp. 

8762040201 162435 Par. truculenta Parasudis truculenta Longnose greeneye 

8835441401 169313 Par. acuminatus Pareques acuminatus High-hat 

8835441403 621151 Par. iwamotoi Pareques iwamotoi 

8835441402 169318 Par. umbrosus Pareques umbrosus Cubbyu 

8803011101 165522 Par. brachypterus Parexocoetus brachypterus Sailfin flyingfish 

8803011100 165521 Parexocoetus spp. Parexocoetus spp. 

8792012601 164981 Par. schmidti Parophidion schmidti Dusky cusk-eel 

8792012600 164980 Parophidion spp. Parophidion spp. 

8835470000 169470 Pempheridae spp. Pempheridae spp. 

8835470101 169472 Pem. schomburgkii Pempheris schomburgkii Glassy sweeper 

8835470100 169471 Pempheris spp. Pempheris spp. 

6177010000 95602 Penaeidae spp. Penaeidae spp. 

8851030104 172568 Pep. burti Peprilus burti Gulf butterfish 

8851030106 172570 Pep. paru Peprilus paru Harvestfish 

8851030100 172564 Peprilus spp. Peprilus spp. 

8851030103 172567 Pep. triacanthus Peprilus triacanthus Butterfish 

8835200201 168469 Per. flavescens Perca flavescens 

8835200000 168356 Percidae spp. Percidae spp. 

8835200335 553384 Per. austroperca Percina austroperca 

8835200319 168490 Per. nigrofasciata Percina nigrofasciata Blackbanded darter 

8835200333 168503 Per. vigil Percina vigil 

6179110401 96415 Per. americanus Periclimenes americanus American grass shrimp 

6179110403 96417 Per. longicaudatus Periclimenes longicaudatus Longtail grass shrimp 

6179110400 96414 Periclimenes spp. Periclimenes spp. 

8826020313 167016 Per. brevirostre Peristedion brevirostre 

8826020303 167006 Per. gracile Peristedion gracile 

8826020304 167007 Per. greyae Peristedion greyae 

8826020307 167010 Per. miniatum Peristedion miniatum 

8826020311 167014 Per. thompsoni Peristedion thompsoni 

8603010301 159722 Pet. marinus Petromyzon marinus 

8792012701 164985 Pet. sanguineus Petrotyx sanguineus 

8835180501 168309 Pha. conklini Phaeoptyx conklini Freckled cardinalfish 

8835180503 168311 Pha. pigmentaria Phaeoptyx pigmentaria Dusky cardinalfish 

8835180500 168308 Phaeoptyx spp. Phaeoptyx spp. 

8835180502 168310 Pha. xenus Phaeoptyx xenus Sponge cardinalfish 

8835270301 168578 Pht. lineatus Phtheirichthys lineatus 

8791010308 550838 Phy. fulvus Physiculus fulvus 

8776011602 163517 Pim. promelas Pimephales promelas Fathead minnow 

8776011600 163515 Pimephales spp. Pimephales spp. 

8803020401 165585 Pla. argalus Platybelone argalus Keeltail needlefish


8803020400 165584 Platybelone spp. Platybelone spp. 

6189071701 99076 Pla. spectabilis Platychirograpsus spectabilis Saber crab 

6189071700 99075 Platychirograpsus spp. Platychirograpsus spp. Saber crab 

8840130501 171051 Pla. rubrocinctus Platygillellus rubrocinctus Saddle stargazer 

8835022601 167873 Ple. garrupellus Plectranthias garrupellus 

8810080401 166245 Ple. retrospinis Plectrypops retrospinis Cardinal soldierfish 

8810080400 166244 Plectrypops spp. Plectrypops spp. 

8857040000 615937 Pleuronectidae spp. Pleuronectidae spp. 

8855000000 172702 Pleuronectiformes spp. Pleuronectiformes spp flatfishes 

8804080201 165898 Poe. latipinna Poecilia latipinna Sailfin molly 

8804080205 165903 Poe. reticulata Poecilia reticulata Guppy 

8804080206 165904 Poe. sphenops Poecilia sphenops 

8804080200 165897 Poecilia spp. Poecilia spp. 

8804080000 165876 Poeciliidae spp. Poeciliidae spp. 

8835440801 169288 Pog. cromis Pogonias cromis Black drum 

8835440800 169287 Pogonias spp. Pogonias spp. 

8838010101 170447 Pol. octonemus Polydactylus octonemus Atlantic threadfin 

8838010104 170450 Pol. oligodon Polydactylus oligodon Littlescale threadfi 

8838010100 170446 Polydactylus spp. Polydactylus spp. 

8838010102 170448 Pol. virginicus Polydactylus virginicus Barbu 

8809010101 166126 Pol. lowei Polymixia lowei Beardfish 

8809010102 166127 Pol. nobilis Polymixia nobilis 

8809010100 166125 Polymixia spp. Polymixia spp. 

8809010000 166124 Polymixiidae spp. Polymixiidae spp. 

8838010000 170445 Polynemidae spp. Polynemidae spp. 

8729020101 161088 Pol. spathula Polyodon spathula 

8835580101 167914 Pol. americanus Polyprion americanus Wreckfish 

8835580100 167913 Polyprion spp. Polyprion spp. Wreckfish 

8835550401 169632 Pom. arcuatus Pomacanthus arcuatus Gray angelfish 

8835550404 169635 Pom. imperator Pomacanthus imperator 

8835550402 169633 Pom. paru Pomacanthus paru French angelfish 

8835550400 169631 Pomacanthus spp. Pomacanthus spp. 

8835620000 170044 Pomacentridae spp. Pomacentridae spp. 

8835400502 169093 Pom. crocro Pomadasys crocro Burro grunt 

8835400500 169091 Pomadasys spp. Pomadasys spp. 

8835250000 168557 Pomatomidae spp. Pomatomidae spp. 

8835250101 168559 Pom. saltatrix Pomatomus saltatrix Bluefish 

8835250100 168558 Pomatomus spp. Pomatomus spp. 

8835160701 168166 Pom. annularis Pomoxis annularis White crappie 

8835160702 168167 Pom. nigromaculatus Pomoxis nigromaculatus Black crappie 

8835160700 168165 Pomoxis spp. Pomoxis spp. 

8826010501 166799 Pon. castor Pontinus castor Longsnout scorpionfish


8826010503 166801 Pon. longispinis Pontinus longispinis Longspine scorpionfish 

8826010506 166804 Pon. nematophthalmus Pontinus nematophthalmus Spinythroat scorpion 

8826010505 166803 Pon. rathbuni Pontinus rathbuni Highfin scorpionfish 

8826010500 166798 Pontinus spp. Pontinus spp. 

8783010108 164421 Por. plectrodon Porichthys plectrodon Atlantic midshipman 

8783010100 164413 Porichthys spp. Porichthys spp. 

6189010000 98689 Portunidae spp. Portunidae spp. Swimming crabs 

6189010601 98718 Por. gibbesii Portunus gibbesii 

6189010604 98721 Por. spinimanus Portunus spinimanus Blotched swimming crab 

6189010600 98717 Portunus spp. Portunus spp. 

8835170000 168176 Priacanthidae spp. Priacanthidae spp. 

8835170101 168178 Pri. arenatus Priacanthus arenatus Bigeye 

8835170100 168177 Priacanthus spp. Priacanthus spp. 

8847082701 172234 Pri. hipoliti Priolepis hipoliti 

8708020601 160424 Pri. glauca Prionace glauca 

8826020105 166978 Pri. alatus Prionotus alatus 

8826020101 166974 Pri. carolinus Prionotus carolinus Northern searobin 

8826020102 166975 Pri. evolans Prionotus evolans Striped searobin 

8826020123 166996 Pri. longispinosus Prionotus longispinosus Bigeye Searobin 

8826020111 166984 Pri. martis Prionotus martis Barred searobin 

8826020113 166986 Pri. ophryas Prionotus ophryas Bandtail searobin 

8826020117 166990 Pri. roseus Prionotus roseus Bluespotted searobin 

8826020118 166991 Pri. rubio Prionotus rubio Blackfin searobin 

8826020103 166976 Pri. scitulus Prionotus scitulus Leopard searobin 

8826020100 166973 Prionotus spp. Prionotus spp. 

8826020121 166994 Pri. stearnsi Prionotus stearnsi Shortwing searobin 

8826020104 166977 Pri. tribulus Prionotus tribulus Bighead searobin 

8713010000 160807 Pristidae spp. Pristidae spp. 

8835170201 168190 Pri. alta Pristigenys alta Short bigeye 

8835170200 168188 Pristigenys spp. Pristigenys spp. 

8835360701 168913 Pri. aquilonaris Pristipomoides aquilonaris Wenchman 

8835360702 168914 Pri. freemani Pristipomoides freemani 

8835360700 168912 Pristipomoides spp. Pristipomoides spp. 

8713010101 160809 Pri. pectinata Pristis pectinata Smalltooth sawfish 

8713010103 160810 Pri. pristis Pristis pristis Largetooth sawfish 

8713010100 160808 Pristis spp. Pristis spp. 

8835550135 169592 Pro. aculeatus Prognathodes aculeatus Longsnout butterflyfish 

8835550102 169557 Pro. aya Prognathodes aya Bank butterflyfish 

8835550501 621153 Pro. guyanensis Prognathodes guyanensis 

8803011201 165524 Pro. occidentalis Prognichthys occidentalis Bluntnose flyingfish 

8803011200 165523 Prognichthys spp. Prognichthys spp. 

8835023401 167931 Pro. martinicensis Pronotogrammus martinicensis Roughtongue bass


8835023400 167930 Pronotogrammus spp. Pronotogrammus spp. 

8851020401 172556 Pse. cyanophrys Psenes cyanophrys Freckled driftfish 

8851020402 172557 Psen. maculatus Psenes maculatus Silver driftfish 

8851020403 172558 Pse. pellucidus Psenes pellucidus Bluefin driftfish 

8851020400 172555 Psenes spp. Psenes spp. 

9002030804 173813 Pse. nelsoni Pseudemys nelsoni Florida redbelly turtle 

9002030803 208648 Pse. peninsularis Pseudemys peninsularis Peninsula cooter 

9002030800 173803 Pseudemys spp. Pseudemys spp. Cooters - turtles 

9002030802 208646 Pse. suwanniensis Pseudemys suwanniensis Suwannee cooter 

8835030502 168001 Pse. gregoryi Pseudogramma gregoryi Reef bass 

8835030500 167999 Pseudogramma spp. Pseudogramma spp. 

8741131402 161486 Pse. fugesae Pseudomyrophis fugesae 

8835450301 169421 Pse. maculatus Pseudupeneus maculatus Spotted goatfish 

8835450300 169420 Pseudupeneus spp. Pseudupeneus spp. Goatfish 

8847011401 171866 Pte. calliura Ptereleotris calliura Blue dartfish 

8847014304 621161 Pte. helenae Ptereleotris helenae 

8847011400 171865 Ptereleotris spp. Ptereleotris spp. Dartfishes 

8826011401 166883 Pte. volitans Pterois volitans 

8776018906 621142 Pte. grandipinnis Pteronotropis grandipinnis 

8776018903 201941 Pte. hypselopterus Pteronotropis hypselopterus Sailfin shiner 

8776018904 201942 Pte. signipinnis Pteronotropis signipinnis Flagfin shiner 

8776018905 201943 Pte. welaka Pteronotropis welaka Bluenose shiner 

8713050112 621026 Pte. violacea Pteroplatytrygon violacea 

8835710301 170297 Pte. brama Pterycombus brama Atlantic fanfish 

8835710300 170296 Pterycombus spp. Pterycombus spp. 

8777302001 553392 Pte. disjunctivus Pterygoplichthys disjunctivus 

8777301002 164375 Pte. multiradiatus Pterygoplichthys multiradiatus Armoured catfish 

8777301000 164372 Pterygoplichthys spp. Pterygoplichthys spp. Armoured catfish 

8777020301 164029 Pyl. olivaris Pylodictis olivaris Flathead catfish 

8777020300 164028 Pylodictis spp. Pylodictis spp. 

8835260000 168564 Rachycentridae spp. Rachycentridae spp. 

8835260101 168566 Rac. canadum Rachycentron canadum Cobia 

8835260100 168565 Rachycentron spp. Rachycentron spp. 

8713040117 160859 Raj. ackleyi Raja ackleyi Ocellate skate 

8713040113 160855 Raj. eglanteria Raja eglanteria Clearnose skate 

8713040100 160846 Raja spp. Raja spp. 

8713040133 160875 Raj. texana Raja texana Roundel skate 

8713040000 160845 Rajidae spp. Rajidae spp. 

8815030101 166356 Reg. glesne Regalecus glesne 

8835270501 168582 Rem. albescens Remora albescens White suckerfish 

8835270104 168572 Rem. brachyptera Remora brachyptera Spearfish remora 

8835270102 168570 Rem. osteochir Remora osteochir Marlinsucker


8835270103 168571 Rem. remora Remora remora Remora 

8835270100 168568 Remora spp. Remora spp. 

8713020000 160813 Rhinobatidae spp. Rhinobatidae spp. 

8713020101 160815 Rhi. lentiginosus Rhinobatos lentiginosus 

8713020100 160814 Rhinobatos spp. Rhinobatos spp. 

8713070301 160985 Rhi. bonasus Rhinoptera bonasus Cownose ray 

8713070300 160984 Rhinoptera spp. Rhinoptera spp. 

8708020303 160206 Rhi. porosus Rhizoprionodon porosus 

8708020300 160199 Rhizoprionodon spp. Rhizoprionodon spp. 

8708020301 160200 Rhi. terraenovae Rhizoprionodon terraenovae Atlantic sharpnose shark 

8835360501 168909 Rho. aurorubens Rhomboplites aurorubens Vermilion snapper 

8835360500 168908 Rhomboplites spp. Rhomboplites spp. 

8741121001 161388 Rhy. flavus Rhynchoconger flavus Yellow conger 

8741121002 161390 Rhy. gracilior Rhynchoconger gracilior Whiptail conger 

8741121000 161387 Rhynchoconger spp. Rhynchoconger spp. 

6177010201 551662 Rim. constrictus Rimapenaeus constrictus Roughneck shrimp 

6177010200 551562 Rimapenaeus spp. Rimapenaeus spp. 

8847011501 171869 Ris. ruber Risor ruber Tusked goby 

8847011500 171868 Risor spp. Risor spp. 

8804041001 165698 Riv. marmoratus Rivulus marmoratus Rivulus 

8804041000 165697 Rivulus spp. Rivulus spp. 

8850010401 172364 Ruv. pretiosus Ruvettus pretiosus 

8835030202 167985 Ryp. bistrispinus Rypticus bistrispinus Freckled soapfish 

8835030204 167987 Ryp. maculatus Rypticus maculatus Whitespotted soapfish 

8835030207 167990 Ryp. saponaceus Rypticus saponaceus Greater soapfish 

8835030200 167983 Rypticus spp. Rypticus spp. 

8835030208 167991 Ryp. subbifrenatus Rypticus subbifrenatus Spotted soapfish 

8835200402 168509 San. canadense Sander canadense Sauger 

8835200400 168505 Sander spp. Sander spp. 

8850030202 172409 Sar. sarda Sarda sarda Atlantic bonito 

8850030200 172407 Sarda spp. Sarda spp. 

8747011001 161763 Sar. aurita Sardinella aurita Spanish sardine 

8747011000 161762 Sardinella spp. Sardinella spp. 

8810080110 166178 Sar. bullisi Sargocentron bullisi Deepwater squirrelfish 

8810080111 166180 Sar. coruscum Sargocentron coruscum Reef squirrelfish 

8810080112 166182 Sar. poco Sargocentron poco Saddle squirrelfish 

8810080113 166184 Sar. vexillarium Sargocentron vexillarium Dusky squirrelfish 

8835610403 169812 Sar. melanotheron Sarotherodon melanotheron Blackchin tilapia 

8741100302 161314 Sau. cognita Saurenchelys cognita 

8762020301 162408 Sau. brasiliensis Saurida brasiliensis Largescale lizardfish 

8762020302 162410 Sau. caribbaea Saurida caribbaea Smallscale lizardfish 

8762020303 162411 Sau. normani Saurida normani Shortjaw lizardfish


8762020300 162407 Saurida spp. Saurida spp. 

8839030000 170809 Scaridae spp. Scaridae spp. 

8842012601 171307 Sca. cristata Scartella cristata Molly miller 

8842012600 171306 Scartella spp. Scartella spp. 

8839030102 170812 Sca. coelestinus Scarus coelestinus Midnight parrotfish 

8839030101 170811 Sca. coeruleus Scarus coeruleus Blue parrotfish 

8839030104 170814 Sca. guacamaia Scarus guacamaia Rainbow parrotfish 

8839030103 614740 Sca. iseri Scarus iseri Striped parrotfish 

8839030100 170810 Scarus spp. Scarus spp. 

8839030105 170815 Sca. taeniopterus Scarus taeniopterus Princess parrotfish 

8839030106 170816 Sca. vetula Scarus vetula Queen parrotfish 

8835022101 167846 Sch. beta Schultzea beta 

8835440000 169237 Sciaenidae spp. Sciaenidae spp. 

8835440901 169290 Sci. ocellatus Sciaenops ocellatus Red drum 

8835440900 169289 Sciaenops spp. Sciaenops spp. 

8850030301 172412 Sco. colias Scomber colias Chub mackerel 

8850030300 172411 Scomber spp. Scomber spp. 

8850030501 172435 Sco. cavalla Scomberomorus cavalla King mackerel 

8850030502 172436 Sco. maculatus Scomberomorus maculatus Spanish mackerel 

8850030503 172437 Sco. regalis Scomberomorus regalis Cero 

8850030500 172434 Scomberomorus spp. Scomberomorus spp. 

8850030000 172398 Scombridae spp. Scombridae spp. 

8835250302 621149 Sco. oculatus Scombrops oculatus 

8857020000 172713 Scophthalmidae spp. Scophthalmidae spp. 

8857030401 172746 Sco. aquosus Scophthalmus aquosus Windowpane 

8857030400 172745 Scophthalmus spp. Scophthalmus spp. 

8826010601 166812 Sco. agassizii Scorpaena agassizii Longfin scorpionfish 

8826010602 166813 Sco. albifimbria Scorpaena albifimbria Coral scorpionfish 

8826010603 166814 Sco. bergii Scorpaena bergii Goosehead scorpionfish 

8826010604 166815 Sco. brachyptera Scorpaena brachyptera Shortfin scorpionfish 

8826010605 166816 Sco. brasiliensis Scorpaena brasiliensis Barbfish 

8826010606 166817 Sco. calcarata Scorpaena calcarata Smoothhead scorpionfish 

8826010607 166818 Sco. dispar Scorpaena dispar Hunchback scorpionfish 

8826010608 166819 Sco. elachys Scorpaena elachys Dwarf scorpionfish 

8826010609 166820 Sco. grandicornis Scorpaena grandicornis Plumed scorpionfish 

8826010610 166821 Sco. inermis Scorpaena inermis Mushroom scorpionfisf 

8826010611 166822 Sco. isthmensis Scorpaena isthmensis Smoothcheek scorpionfish 

8826010614 166825 Sco. plumieri Scorpaena plumieri Spotted scorpionfish 

8826010600 166811 Scorpaena spp. Scorpaena spp. 

8826010000 166704 Scorpaenidae spp. Scorpaenidae spp. 

8826011201 166862 Sco. caribbaeus Scorpaenodes caribbaeus Reef scorpionfish 

8826011200 166861 Scorpaenodes spp. Scorpaenodes spp.


8826011202 166863 Sco. tredecimspinosus 

Scorpaenodes 

tredecimspinosus 

Deepreef scorpionfish 

8708010000 159985 Scyliorhinidae spp. Scyliorhinidae spp. 

8708010304 160060 Scy. retifer Scyliorhinus retifer 

6182020000 97660 Scyllaridae spp. Scyllaridae spp. 

6182020203 97676 Scy. aequinoctialis Scyllarides aequinoctialis 

6182020202 97673 Scy. nodifer Scyllarides nodifer 

6182020200 97671 Scyllarides spp. Scyllarides spp. 

6182020104 97667 Scy. americanus Scyllarus americanus 

6182020102 97665 Scy. chacei Scyllarus chacei 

6182020101 97662 Scy. depressus Scyllarus depressus 

6182020105 97670 Scy. faxoni Scyllarus faxoni 

6182020100 97661 Scyllarus spp. Scyllarus spp. 

8835280601 168677 Sel. crumenophthalmus Selar crumenophthalmus Bigeye scad 

8835280600 168676 Selar spp. Selar spp. 

8835280705 168684 Sel. setapinnis Selene setapinnis Atlantic moonfish 

8835280700 168679 Selene spp. Selene spp. 

8835280701 168680 Sel. vomer Selene vomer Lookdown 

8776010800 163374 Semotilus spp. Semotilus spp. 

8776010802 163376 Sem. thoreauianus Semotilus thoreauianus Creek chub 

8835280801 168689 Ser. dumerili Seriola dumerili Greater amberjack 

8835280802 168690 Ser. fasciata Seriola fasciata Lesser amberjack 

8835280803 168691 Ser. rivoliana Seriola rivoliana Almaco jack 

8835280800 168688 Seriola spp. Seriola spp. 

8835280804 168693 Ser. zonata Seriola zonata Banded rudderfish 

8835022201 167848 Ser. pumilio Serraniculus pumilio Pygmy sea bass 

8835022200 167847 Serraniculus spp. Serraniculus spp. 

8835020000 167674 Serranidae spp. Serranidae spp. 

8835022301 167850 Ser. annularis Serranus annularis Orangeback bass 

8835022302 167851 Ser. atrobranchus Serranus atrobranchus Blackear bass 

8835022303 167852 Ser. baldwini Serranus baldwini Lantern bass 

8835022304 167853 Ser. chionaraia Serranus chionaraia Snow bass 

8835022307 167856 Ser. notospilus Serranus notospilus 

8835022308 167857 Ser. phoebe Serranus phoebe Tattler 

8835022300 167849 Serranus spp. Serranus spp. 

8835022309 167858 Ser. subligarius Serranus subligarius Belted sandfish 

8835022310 167859 Ser. tabacarius Serranus tabacarius Tobaccofish 

8835022311 167860 Ser. tigrinus Serranus tigrinus Harlequin bass 

8835022312 167861 Ser. tortugarum Serranus tortugarum Chalk bass 

6177040101 96028 Sic. brevirostris Sicyonia brevirostris Rock Shrimp 

6177040102 96030 Sic. dorsalis Sicyonia dorsalis Lesser rock shrimp 

6177040104 96033 Sic. laevigata Sicyonia laevigata Hardback


6177040107 96036 Sic. parri Sicyonia parri 

6177040100 96027 Sicyonia spp. Sicyonia spp. Rock Shrimp 

6177040103 96031 Sic. typica Sicyonia typica Kinglet rock shrimp 

8777000000 163992 Siluriformes spp. Siluriformes spp. Catfish 

8835430000 169180 Sparidae spp. Sparidae spp. 

8839030401 170862 Spa. atomarium Sparisoma atomarium Green blotch parrotfish 

8839030402 170863 Spa. aurofrenatum Sparisoma aurofrenatum Redbanned parrotfish 

8839030403 170864 Spa. chrysopterum Sparisoma chrysopterum Redtail parrotfish 

8839030404 170865 Spa. radians Sparisoma radians Bucktooth parrotfish 

8839030405 170866 Spa. rubripinne Sparisoma rubripinne Redfin parrotfish 

8839030400 170861 Sparisoma spp. Sparisoma spp. 

8839030406 170867 Spa. viride Sparisoma viride Spotlight parrotfish 

8861010205 173294 Sph. dorsalis Sphoeroides dorsalis Marbled puffer 

8861010201 173290 Sph. maculatus Sphoeroides maculatus Northern puffer 

8861010208 173297 Sph. nephelus Sphoeroides nephelus Southern puffer 

8861010209 173298 Sph. pachygaster Sphoeroides pachygaster Blunthead puffer 

8861010210 173299 Sph. parvus Sphoeroides parvus Least puffer 

8861010211 173300 Sph. spengleri Sphoeroides spengleri Bandtail puffer 

8861010200 173289 Sphoeroides spp. Sphoeroides spp. 

8861010202 173291 Sph. testudineus Sphoeroides testudineus Checkered puffer 

8837010104 170429 Sph. barracuda Sphyraena barracuda Great barracuda 

8837010102 170427 Sph. borealis Sphyraena borealis Northern sennet 

8837010103 170428 Sph. guachancho Sphyraena guachancho Guaguanche 

8837010105 170430 Sph. picudilla Sphyraena picudilla Southern sennet 

8837010100 170425 Sphyraena spp. Sphyraena spp. 

8837010000 170424 Sphyraenidae spp. Sphyraenidae spp. 

8708030103 160508 Sph. lewini Sphyrna lewini Scalloped hammerhead 

8708030104 160515 Sph. mokarran Sphyrna mokarran Great hammerhead 

8708030100 160499 Sphyrna spp. Sphyrna spp. 

8708030101 160502 Sph. tiburo Sphyrna tiburo Bonnethead 

8708030102 160505 Sph. zygaena Sphyrna zygaena Smooth hammerhead 

8708030000 160497 Sphyrnidae spp. Sphyrnidae spp. 

8710010000 160604 Squalidae spp. Squalidae spp. 

8710010201 160617 Squ. acanthias Squalus acanthias Spiny dogfish 

8710010203 160623 Squ. cubensis Squalus cubensis 

8710010205 160625 Squ. mitsukurii Squalus mitsukurii 

8710010200 160616 Squalus spp. Squalus spp. 

8711010102 160787 Squ. dumeril Squatina dumeril 

6191010101 99143 Squ. empusa Squilla empusa Rough mantis shrimp 

6191010100 99142 Squilla spp. Squilla spp. Mantis shrimp 

6191010000 99141 Squillidae spp. Squillidae spp. 

8842090602 171439 Sta. ocellata Starksia ocellata Checkered blenny


8842090600 171437 Starksia spp. Starksia spp. 

8842090603 171440 Sta. starcki Starksia starcki Key blenny 

8842090701 171446 Sta. hemphilli Stathmonotus hemphilli Blackbelly blenny 

8842090700 171445 Stathmonotus spp. Stathmonotus spp. 

8842090702 171447 Sta. stahli Stathmonotus stahli Eelgrass blenny 

8835621229 615332 Ste. adustus Stegastes adustus Dusky damselfish 

8835621202 615394 Ste. diencaeus Stegastes diencaeus Longfin damselfish 

8835621230 615345 Ste. leucostictus Stegastes leucostictus Beaugregory 

8835621234 615416 Ste. partitus Stegastes partitus Bicolor damselfish 

8835621232 615485 Ste. planifrons Stegastes planifrons Threespot damselfish 

8835621200 170150 Stegastes spp. Stegastes spp. 

8835621233 615412 Ste. variabilis Stegastes variabilis Cocoa damselfish 

8791040201 164802 Ste. argentea Steindachneria argentea 

8835441001 169292 Ste. lanceolatus Stellifer lanceolatus Star drum 

8835441000 169291 Stellifer spp. Stellifer spp. 

8835430102 169183 Ste. caprinus Stenotomus caprinus Longspine porgy 

8835430101 169182 Ste. chrysops Stenotomus chrysops Scup 

8835430100 169181 Stenotomus spp. Stenotomus spp. 

8860020703 173183 Ste. hispidus Stephanolepis hispidus Planehead filefish 

8860020704 173184 Ste. setifer Stephanolepis setifer Pygmy filefish 

9002020104 173761 Ste. minor Sternotherus minor Loggerhead musk turtle 

9002020101 173758 Ste. odoratus Sternotherus odoratus Stinkpot 

9002020100 173757 Sternotherus spp. Sternotherus spp. Musk turtles 

3737040301 51926 Sto. meleagris Stomolophus meleagris Cannonball jellyfish 

8851030000 172563 Stromateidae spp. Stromateidae spp. 

8803020201 165551 Str. marina Strongylura marina Atlantic needlefish 

8803020202 165553 Str. notata Strongylura notata Redfin needlefish 

8803020200 165550 Strongylura spp. Strongylura spp. 

8803020203 165554 Str. timucu Strongylura timucu Timucu 

8792012801 164988 Sty. latebricola Stygnobrotula latebricola Black brotula 

8792012800 164987 Stygnobrotula spp. Stygnobrotula spp. 

8816010000 166358 Stylephoridae spp. Stylephoridae spp. 

8816010101 166360 Sty. chordatus Stylephorus chordatus Tube-eye 

8816010100 166359 Stylephorus spp. Stylephorus spp. 

8857031301 172791 Sya. gunteri Syacium gunteri Shoal flounder 

8857031302 172792 Sya. micrurum Syacium micrurum Channel flounder 

8857031303 172793 Sya. papillosum Syacium papillosum Dusky flounder 

8857031300 172790 Syacium spp. Syacium spp. 

8858020113 173074 Sym. arawak Symphurus arawak Caribbean tonguefish 

8858020102 616615 Sym. civitatium Symphurus civitatium Offshore tonguefish 

8858020103 616664 Sym. diomedeanus Symphurus diomedeanus Spottedfin tonguefish 

8858020104 173065 Sym. marginatus Symphurus marginatus


8858020105 173066 Sym. minor Symphurus minor Largescale tonguefish 

8858020114 173075 Sym. nebulosus Symphurus nebulosus 

8858020106 173067 Sym. parvus Symphurus parvus Pygmy tonguefish 

8858020115 173076 Sym. pelicanus Symphurus pelicanus Longtail tonguefish 

8858020107 173068 Sym. piger Symphurus piger 

8858020101 173062 Sym. plagiusa Symphurus plagiusa Blackcheek tonguefish 

8858020100 173061 Symphurus spp. Symphurus spp. 

8858020121 616519 Sym. stigmosus Symphurus stigmosus 

8858020110 173071 Sym. urospilus Symphurus urospilus Spottail tonguefish 

8741170000 161595 Synaphobranchidae spp. Synaphobranchidae spp. 

8824010000 166692 Synbranchidae spp. Synbranchidae spp. Swamp eels 

8820020000 166443 Syngnathidae spp. Syngnathidae spp. 

8820020102 166446 Syn. floridae Syngnathus floridae Dusky pipefish 

8820020103 166451 Syn. fuscus Syngnathus fuscus Northern pipefish 

8820020104 166452 Syn. louisianae Syngnathus louisianae Chain pipefish 

8820020108 166454 Syn. pelagicus Syngnathus pelagicus Sargassum pipefish 

8820020113 166458 Syn. scovelli Syngnathus scovelli Gulf pipefish 

8820020100 166444 Syngnathus spp. Syngnathus spp. 

8820020109 166455 Syn. springeri Syngnathus springeri Bull pipefish 

8762020000 162374 Synodontidae spp. Synodontidae spp. 

8762020101 162376 Syn. foetens Synodus foetens Inshore lizardfish 

8762020102 162377 Syn. intermedius Synodus intermedius Sand diver 

8762020104 162379 Syn. poeyi Synodus poeyi 

8762020105 162380 Syn. saurus Synodus saurus 

8762020100 162375 Synodus spp. Synodus spp. 

8762020106 162382 Syn. synodus Synodus synodus Red lizardfish 

8835710701 170311 Tar. longipinnis Taractichthys longipinnis Bigscale pomfret 

8835710700 170310 Taractichthys spp. Taractichthys spp. 

9002000000 173749 Testudines spp. Testudines spp. Turtles 

8851040102 172581 Tet. atlanticus Tetragonurus atlanticus 

8861010000 173283 Tetraodontidae spp. Tetraodontidae spp. 

8850060301 172499 Tet. albidus Tetrapturus albidus White marlin 

8850060305 172503 Tet. angustirostris Tetrapturus angustirostris Shortbill spearfish 

8850060304 172502 Tet. pfluegeri Tetrapturus pfluegeri Longbill spearfish 

8850060300 172498 Tetrapturus spp. Tetrapturus spp. 

8839011001 170568 Tha. bifasciatum Thalassoma bifasciatum 

6179161404 96921 Tho. dobkini Thor dobkini Squat grass shrimp 

6179161402 96919 Tho. manningi Thor manningi Manning grass shrimp 

8850030401 172419 Thu. alalunga Thunnus alalunga Albacore 

8850030403 172423 Thu. albacares Thunnus albacares Yellowfin tuna 

8850030404 172427 Thu. atlanticus Thunnus atlanticus Blackfin tuna 

8850030405 172428 Thu. obesus Thunnus obesus Bigeye tuna


8850030400 172418 Thunnus spp. Thunnus spp. 

8850030402 172421 Thu. thynnus Thunnus thynnus Bluefin tuna 

8835610402 169811 Til. mariae Tilapia mariae Spotted tilapia 

8835610400 169809 Tilapia spp. Tilapia spp. 

8713030000 160829 Torpedinidae spp. Torpedinidae spp. 

8713030103 160835 Tor. andersoni Torpedo andersoni 

8713030102 160834 Tor. nobiliana Torpedo nobiliana Atlantic torpedo 

8713030100 160830 Torpedo spp. Torpedo spp. 

6179161201 96912 Toz. carolinense Tozeuma carolinense Arrow shrimp 

6179161200 96911 Tozeuma spp. Tozeuma spp. Arrow shrimp 

900203090102 173823 Tra. scripta elegans Trachemys scripta elegans Red-eared turtle 

900203090101 173821 Tra. scripta scripta Trachemys scripta scripta Yellow bellied turtle 

9002030900 173818 Trachemys spp. Trachemys spp. Sliders 

8762020401 162420 Tra. myops Trachinocephalus myops Snakefish 

8762020400 162419 Trachinocephalus spp. Trachinocephalus spp. 

8835280901 168708 Tra. carolinus Trachinotus carolinus Florida pompano 

8835280902 168709 Tra. falcatus Trachinotus falcatus Permit 

8835280903 168710 Tra. goodei Trachinotus goodei Palometa 

8835280900 168707 Trachinotus spp. Trachinotus spp. 

8815020000 166339 Trachipteridae spp. Trachipteridae spp. 

8815020102 166342 Tra. arcticus Trachipterus arcticus Dealfish 

8815020100 166340 Trachipterus spp. Trachipterus spp. 

8835280102 168587 Tra. lathami Trachurus lathami Rough scad 

8835280100 168585 Trachurus spp. Trachurus spp. 

8826011101 166859 Tra. cristulata Trachyscorpia cristulata 

8708040000 160529 Triakidae spp. Triakidae spp. 

8850020000 172378 Trichiuridae spp. Trichiuridae spp. 

8850020201 172385 Tri. lepturus Trichiurus lepturus Atlantic cutlassfish 

8850020200 172384 Trichiurus spp. Trichiurus spp. 

8857031400 172794 Trichopsetta spp. Trichopsetta spp. 

8857031404 172798 Tri. ventralis Trichopsetta ventralis Sash flounder 

8852021003 553301 Tri. vittata Trichopsis vittata 

8858030102 172983 Tri. inscriptus Trinectes inscriptus Scrawled sole 

8858030101 172982 Tri. maculatus Trinectes maculatus Hogchoker 

8858030100 172981 Trinectes spp. Trinectes spp. 

9002060000 173845 Trionychidae spp. Trionychidae spp. Softshell turtles 

8803020301 165571 Tyl. acus Tylosurus acus Agujon 

8803020302 165577 Tyl. crocodilus Tylosurus crocodilus Houndfish 

8803020300 165570 Tylosurus spp. Tylosurus spp. 

8758020101 162148 Umb. pygmaea Umbra pygmaea 

8835441101 169298 Umb. coroides Umbrina coroides Sand drum 

8835441100 169297 Umbrina spp. Umbrina spp.


9999000000 9999 Unidentified species Unidentified species 

8835450402 169441 Upe. parvus Upeneus parvus Dwarf goatfish 

8835450400 169439 Upeneus spp. Upeneus spp. 

8840140000 171053 Uranoscopidae spp. Uranoscopidae spp. 

8835281701 168746 Ura. secunda Uraspis secunda Cottonmouth jack 

8835281700 168745 Uraspis spp. Uraspis spp. 

8713050301 621035 Uro. jamaicensis Urobatis jamaicensis Yellow stingray 

8713050300 160964 Urobatis spp. Urobatis spp. 

8741120800 161382 Uroconger spp. Uroconger spp. 

8741120801 161383 Uro. syringinus Uroconger syringinus Threadtail conger 

8791031004 164733 Uro. chesteri Urophycis chesteri 

8791031005 164735 Uro. cirrata Urophycis cirrata 

8791031006 164736 Uro. earllii Urophycis earllii Carolina hake 

8791031007 164737 Uro. floridana Urophycis floridana Southern hake 

8791031002 164731 Uro. regia Urophycis regia Spotted hake 

8791031000 164729 Urophycis spp. Urophycis spp. 

8741050715 161262 Uro. macularius Uropterygius macularius Marbled moray 

8741050700 161246 Uropterygius spp. Uropterygius spp. 

8847040102 172157 Var. marilynae Varicus marilynae 

8860020801 173187 Xan. ringens Xanthichthys ringens Sargassum triggerfish 

8860020800 173186 Xanthichthys spp. Xanthichthys spp. 

8811040202 166302 Xen. dalgleishi Xenolepidichthys dalgleishi 

8741120902 161386 Xen. congroides Xenomystax congroides 

8850040101 172482 Xip. gladius Xiphias gladius Swordfish 

8850040100 172481 Xiphias spp. Xiphias spp. Swordfishes 

6177010701 95750 Xip. kroyeri Xiphopenaeus kroyeri Atlantic seabob 

6177010700 95749 Xiphopenaeus spp. Xiphopenaeus spp. Seabob 

8804080601 165920 Xip. hellerii Xiphophorus hellerii 

8804080602 165922 Xip. maculatus Xiphophorus maculatus 

8804080600 165919 Xiphophorus spp. Xiphophorus spp. 

8804080603 165925 Xip. variatus Xiphophorus variatus 

8839010801 614502 Xyr. martinicensis Xyrichtys martinicensis Rosy razorfish 

8839010802 614513 Xyr. novacula Xyrichtys novacula Pearly razorfish 

8839010804 614546 Xyr. splendens Xyrichtys splendens Green razorfish 

8839010800 613036 Xyrichtys spp. Xyrichtys spp. 

8787040401 164597 Zal. mcgintyi Zalieutes mcgintyi 

8811020101 166277 Zen. hololepis Zenion hololepis 

8811020100 166276 Zenion spp. Zenion spp. 

8811020000 166275 Zenionidae spp. Zenionidae spp. 

8811030202 166284 Zen. conchifera Zenopsis conchifera 

8815020301 166353 Zu. cristatus Zu cristatus Scalloped ribbonfish 

8815020300 166352 Zu spp. Zu spp.

Page 1 

Section 8.0 

April 2008 


8.0 Ichthyoplankton and Zooplankton 

The primary objective of this HBMP element is to develop comprehensive, long-term 

measurements of species composition (to the lowest practical identifiable level), 

abundance and distribution of ichthyoplankton and zooplankton within each of the 

HBMP reporting units. This section describes activities being conducted by Dr. Ernst 

Peebles and his research staff at the University of South Florida. 

Patterns in the distribution of ichthyoplankton (fish eggs, larvae, and small juveniles) 

reflect local fish spawning activity and also the ingression of larvae and juveniles into 

inshore nursery habitats from more distant spawning locations. Ingression takes the form 

of passive transport, active migration, or a combination of the two processes. Freshwater 

inflows influence spawning activity, larval transport mechanisms, and the distribution of 

larvae and juveniles within estuarine nursery habitats. Upon arrival in the estuarine 

nursery, some fish species prefer structured, stationary habitats (e.g., mangrove roots, 

seagrasses, oyster reefs, marsh edges, etc.) and exhibit relatively small distributional 

responses to inflow variation, whereas others, particularly those that occupy unstructured 

channel areas, shift position readily as inflows vary. 

In the process of monitoring the distribution and abundance responses of ichthyoplankton 

to freshwater inflows, large numbers of invertebrate zooplankton are also collected. Diet 

studies have shown that many of these organisms serve as important prey for the young 

fishes that occupy estuarine nursery habitats. The distributions and numbers of many of 

these invertebrate prey groups may also change in response to freshwater inflow 

variation. Distributional shifts, in turn, may cause prey to be centered upstream or 

downstream of the fishes’ preferred habitat, particularly with species that are strongly 

associated with stationary habitats. Using regression models, the spatial overlap between 

predator and prey can be estimated as a function of either freshwater inflow or a 

surrogate parameter such as isohaline position. This process incorporates data from both 

the juvenile/adult fish monitoring task (conducted by FWRI) and the 

ichthyoplankton/other zooplankton task. Over the past two decades, Dr. Peebles has 

gained extensive experience investigating these relationships within tidal rivers on 

Florida’s west coast. 

8.1 Project Management 

The overall general organization of the USF zooplankton monitoring element of the 

HBMP is outlined in Figure 8.1. There are three critical points for QA/QC feedback: at 

the Lab Manager position, at the Field Operations Manager position, and at the Database 

Manager position. The Lab Manager position is responsible for QA/QC at the Research 

Assistant and Lab Technician positions. The Field Operations Manager is responsible for 

QA/QC at the Marine Technician position. The specific responsibilities for each of the

Page 2 

Section 8.0 

April 2008 


key positions are described below with regard to project-specific portions of the 

described standard operating procedures (SOP). 

8.2 Key Professional and Technical Staff 

8.2.1 Principal Investigator 

USF title: 

Current Personnel: 

8.2.2 Lab Manager 

USF title: 


Research Associate (Staff) or Faculty 

Ernst Peebles, Ph.D. (Marine Science), 24 years of experience with 

ichthyoplankton and fish diet surveys (19 years as PI, 3 years as 

Research Assistant, 2 years as Marine Technician). Specialist in 

identification of estuarine fish eggs, larvae, juveniles, and fish 

stomach contents. 

Research Associate (Staff ) or Faculty 


analysis of zooplankton samples. Specialist in identification of 

estuarine fish eggs, larvae, and juveniles. Supervisor for 10 

employees at USF. 

8.2.3 Research Assistant 

Scott Burghart, Ph.D. (Marine Science), 14 years of experience 

with collection and analysis of zooplankton and micronekton 

samples. Specialist in identification of copepods and decapod 

larvae. 

USF title: 


Research Assistant (Staff or OPS Staff) 

Ralph Kitzmiller, B.S. (Zoology), 10 years of laboratory 

experience with zooplankton surveys. 

8.2.4 Lab Technician 

USF title: 


Research Assistant (OPS Staff) 

James Locascio, B.S. (Marine Biology), Ph.D. student 

8.2.5 Field Operations Manager 

USF title: 


Research Assistant (Staff or OPS Staff) 

Ralph Kitzmiller, B.S. (Biology), 10 years of field experience with 

zooplankton surveys, including use of water quality instruments.

Page 3 

Section 8.0 

April 2008 


8.2.6 Marine Technician 

USF title : 


Research Assistant (Staff) 

Scott Stahl, B.S. (Biology) 

Keith Fischer, B.S. (Biology) 

James Locascio, B.S. (Biology) 

Jenna Tortorelli, B.S. pending (Marine Biology) 

8.2.7 Database Manager 

USF title: 


Research Associate (Staff or OPS Staff) 


environmental monitoring databases. 

8.3 Problem Definition/Background 

Quantitative sampling of ichthyoplankton and zooplankton is being implemented as one 

of the elements of the HBMP. The overall objective of this element is to assess the role 

of freshwater inflows on estuarine ecosystems, particularly larval stage fishes and 

associated prey, and to detect any ecological change in such ecosystems that result, either 

directly or indirectly, from freshwater regulation practices. 

8.4 Element Description 

Monthly collections with a plankton net will be made in accordance with the Scope of 

Work (see Section 8.9) defined in contracts between PBS&J and the University of South 

Florida College of Marine Science (USF). The samples produced by these collections 

will be processed for taxonomic composition, as described in the Scope of Work, and the 

resulting data will be verified and submitted to PBS&J. The verified data will include 

taxonomic composition data and water quality data associated with individual collections. 

Application of the data to resolving the problem defined above will be the responsibility 

of PBS&J, and is therefore not included within this project description. 

8.5 Quality Objectives and Criteria for Measurement Data 

The following quality objectives and criteria have been established for this element and 

include: 

1. Water quality measurements associated with plankton collections - the objective is to 

achieve levels of accuracy that are of the same level as measurement resolutions for 

the instruments being used to make the measurements. This objective includes 

preventing inaccuracies due to improper calibration, maintenance, or deployment of 

the instruments. The criteria for this objective are the accuracy specifications 

provided by instruments’ manufacturer, which will be verified in the laboratory using

Page 4 

Section 8.0 

April 2008 


reference standards traceable to N.I.S.T. standards. Selection of the instruments and 

their associated, established levels of accuracy will be approved by PBS&J. Postcalibration 

errors >10% will result in data rejection. 

2. Taxonomic composition - the objective is to minimize errors in taxonomic 

identification and enumeration methods. The implicit criterion for acceptable 

taxonomic accuracy is attainment of 100% accuracy. Periodic independent review of 

taxonomic designations will be used to assess actual accuracy. Any exposed errors 

will be resolved by re-sorting or by reduction or correction of the expressed 

taxonomic resolution for the questioned taxa. Because the natural variability in the 

instantaneous abundances of most planktonic organisms spans several orders of 

magnitude, enumeration within plankton samples frequently requires occasional, 

periodic, or routine application of sample splitting procedures for particular taxa. Reenumeration 

of split samples will be used to establish the degree of variability in this 

process, and maintenance of enumeration variability below 20% will be the criterion 

used for split-sample enumeration accuracy. This percentage is based on studies of 

this type of error for similar data that were collected by USF. 

8.6 Special Training 

Training will be performed through personal instruction and will be maintained through 

review of written SOPs, this QA/QC Plan and reference materials. The Primary 

Taxonomist position requires a considerable amount of specialized training that is largely 

obtained through first-hand experience. Development of written and photographic 

materials relevant to taxonomic training will continue at USF during this project, 

facilitating: 1) specialized taxonomic training, 2) attainment of QA/QC objectives such as 

the independent review of taxonomic designations, and 3) a record of taxonomic 

identities that can be maintained in the event of personnel replacement at the Primary 

Taxonomist position. 

8.7 Documents and Records 

Dated, current copies of this QA/QC Plan, project-specific SOPs, and a Data Dictionary 

will be maintained in the Estuarine Ecology Laboratory (EEL) at USF. All personnel 

will be instructed as to the availability of these documents as well as to the requirement 

for all personnel to maintain familiarity with the contents of these documents. Document 

version supercession will conform to date of publication. Current copies of the QA/QC 

Plan, relevant SOPs, and the Data Dictionary will be made available to PBS&J as 

required and upon delivery of data to PBS&J. 

Data will be reported as Microsoft Access electronic files. The reported data will 

include verified water quality data and field collection information, including calculated 

volumes filtered by the plankton net and numbers of individual taxa identified from each 

sample. Original field logs, laboratory data logs, instrument calibration logs, and QC 

check logs will be maintained as hard copies in the EEL.

Page 5 

Section 8.0 

April 2008 


Progress reports will be submitted to PBS&J at intervals corresponding to invoicing 

periods, unless otherwise specified in the contract between USF and PBS&J. Difficulties 

encountered in such reports will be described in language that can be readily interpreted 

using terminology that is defined in the Scope of Work, project-specific SOPs, the Data 

Dictionary, or this QA/QC Plan. 

Records relevant to this project, including original logs, will be retained for a period of 5 

years after completion of the project. 

8.8 Data Generation And Acquisition 

8.8. 1 Sampling Process Design 

8.8.1.1 Minimum Sample Size 

A plankton net will be used to collect zooplankton samples from each of four reporting 

units defined in the HBMP: the lower Hillsborough River, the TBC/Palm River, McKay 

Bay, and the lower Alafia River. During HBMP development, a minimum of 30 samples 

per quarter from each reporting unit was identified as the number of samples required to 

permit meaningful statistical inference on a quarterly basis. When possible, the minimum 

number of samples is to be distributed among the three months composing each quarter. 

In effect, this results in continuous monthly sampling that can be post-stratified into 

quarterly data sets having the desired minimum sample size of 30. 

8.8.1.2 Collection Dates and Times 

Many types of zooplankton and vertically migrating epibenthos have been shown to 

selectively move into the water column at certain times of day and/or tide stages. Effort 

was made to hold these known causes of variation in organism abundance constant 

during zooplankton sampling, thereby removing trends in apparent abundance that are 

related to these behaviors instead of the effects of freshwater inflows. Nighttime 

zooplankton catches are known to be generally larger than daytime catches. This 

phenomenon was confirmed during preliminary sampling of the lower Alafia River. 

Similarly, existing data indicate that, during flood tides, the estuarine water column tends 

to contain more organisms that are moving upstream or are trying to maintain position 

within the estuary, whereas ebb tidal waters tend to contain more organisms that are in 

the process of leaving the estuary. Night-time flood tides were therefore chosen as the 

standard conditions for zooplankton sampling. Dates of sampling, as a result, are 

controlled by the occurrence of nighttime flood tides. 

8.8.1.3 Collection Locations 

The sampled locations within each reporting unit were selected using a one time 

stratified-random approach. Stratification was designed to encourage sampling from all

Page 6 

Section 8.0 

April 2008 


parts of each reporting unit. Randomization was not repeated for each collection date due 

to logistic constraints on gear deployment. Collection locations are located using a GPS 

plotter. 

8.8.2 Sampling Methods 

The standard collection gear consists of a 500 µm Nitex mesh, 0.5 m mouth diameter, 

conical (3:1) plankton net, equipped with a three-point nylon bridle, a 1-liter plastic codend 

jar, 20 kg of weight suspended from the mouth ring by a stainless steel carabiner, and 

a mechanical flowmeter suspended at the center of the net’s mouth by a snap-on 

monofilament bridle. The net’s panel seams are calibrated in liters using permanent ink 

to indicate volumes in excess of the cod-end jar’s capacity. 

Standard deployment consists of a three-step oblique tow that divides the net’s fishing 

time equally between bottom, mid-depth, and surface waters. Tow duration is 5 minutes 

and tow speed is 1.0-1.5 m/s. Net position in the water column is maintained using a 

gunnel-mounted boat trailer winch modified to provide a 1:1 gear ratio. The tow line is 

marked with heat-shrink tubing to indicate collection depth (in meters) and is also 

marked with a reference point that can be used to calibrate the speed of the tow vessel. 

Tow lines are calibrated for specific tow speeds. Flowmeter readings are recorded before 

and after deployment. Upon retrieval, the net is suspended vertically and the meshes are 

systematically and thoroughly rinsed from the outside using ambient water delivered via 

a high pressure, on-demand washdown pump and hose equipped with an adjustable spray 

nozzle. The final volume within the cod-end jar is reduced by draining excess water 

through a small area of mesh in the throat of the net followed by a second rinse of the 

throat area using approximately 500-800 ml of water. The sample is preserved by adding 

50 ml of formaldehyde solution (37% V/V) to the 500-800 sample volume. 

Ctenophore (comb jelly) volume occasionally exceeds the cod-end jar’s capacity. In such 

cases, markings on the net panel seams are used to estimate the total volume of 

ctenophores. If the ctenophore volume is _3.0 liters, then a second 800 ml sample is taken from the 

“upper” part of the catch. The actual volume of the preserved ctenophore catch is 

determined at a later time in the laboratory, and a multiplier is then calculated to correct 

for the discarded portion of the sample. 

Leaves, sticks, mangrove seedlings, and other non-animal items that will not fit into the 

cod-end jar are removed from the lower part of the net and placed in a 4 mm mesh sieve 

for rinsing. The cod-end jar is re-attached to the net if necessary and the contents of the 

sieve are thoroughly rinsed, with the rinse water falling into the interior of the plankton 

net. The rinsed items are then discarded and the net is processed in the standard method 

described above.

Page 7 

Section 8.0 

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The samples are preserved in the plastic cod-end jars in which they were originally 

collected. This reduces both processing time in the field and the potential for 

contaminating samples with organisms from other locations. Two methods are used to 

mark the cod-end jars with unique identifiers: a computer-generated adhesive label is 

placed on the cod-end jar lid, and the same unique identifier is copied onto the side of the 

jar using permanent ink. All jars are pre-labeled in the laboratory to avoid moisturerelated 

problems with label or ink adhesion. The unique identifiers consist of alternating 

alphabetic and numeric characters. This alternation provides a place-holding 

characteristic in the identifier that would not be provided by an entirely alphabetic or 

entirely numeric identifier system. The place-holding feature greatly facilitates 

consistent sample recognition in the field and laboratory. 

Water quality profiles are taken in association with each plankton net deployment. 

Temperature, conductivity, pH and dissolved oxygen are measured electronically at onemeter 

intervals between surface and bottom. The electronic meters are calibrated before 

and after use according to the manufacturers’ specifications. 

8.8.3 Sample Handling and Custody 

The preserved samples are stored in ice chests as they are collected and are returned 

directly to the laboratory immediately after sampling, where they are stored in a fume 

hood. After a minimum of four days in formalin, the samples are transferred to a 250 µm 

mesh sieve (one half the mesh size opening of the collection gear), rinsed with tap water, 

and then returned to their original cod-end jar using a rinse of 50% 2-propanol in deionized 

water. At this point the samples are transferred from the fume hood to a shelf 

near the staging area, which is a dedicated bench top where samples are prepared for 

analysis. 

8.8.4 Analytical Methods 

At a sink near the staging area, the samples are divided into two size fractions using tap 

water and two stacked sieves having 4 mm and 250 µm mesh openings. This process 

removes larger items to prevent them from obscuring smaller items during subsequent 

analysis. Sample analysis consists of determining taxonomic composition, which is 

typically expressed as the number of each taxon per unit volume of ambient water 

filtered. The >4 mm fraction can usually be analyzed without the aid of a microscope. 

After sieving, the contents of the 4 mm sieve are returned to the original cod-end jar and 

the contents of the 250 µm sieve are transferred to a numbered beaker (Fig. 8.2). The 

unique sample identifier (the sample number), the number of the beaker containing the 

250 µm - 4 mm fraction, and the taxonomic composition of the >4 mm fraction are 

recorded on a pre-formatted data sheet (Form LFD, see Table 8.1). Confusion of sample 

identity after fractionation is prevented by a redundant system of sample arrangement 

(Fig. 8.2) and record keeping on Form LFD.

Page 8 

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April 2008 


A zoom 7-90X stereomicroscope equipped with a brightfield/darkfield illumination base 

and an accessory fiber optic light source is used by the Primary Taxonomist to analyze 

the 250 µm - 4 mm fraction. Successive aliquots are poured from the numbered beaker 

into a 90 mm square petri dish delineated into 6 x 6, 13.5 mm square grids. Each aliquot 

is methodically examined by the Primary Taxonomist, who uses the grid delineations to 

define the processing path. The petri dish is marked with arrows to indicate processing 

direction within the dish. After each aliquot has been processed, the contents of the dish 

are rinsed into a 1000 ml graduated cylinder using 50% 2-propanol in de-ionized water. 

Taxonomic data for each sample are entered into a Lotus 1-2-3 spreadsheet directly 

from the microscope workstation. As each sample is processed, a list of encountered taxa 

is constructed within a specialized template area of the spreadsheet. The list is compiled 

as a subset of a master list of taxonomic descriptions and associated numeric codes. 

Macros allow the Primary Taxonomist to select individual taxonomic descriptions/codes 

from the master list for copying to the template area. The template area is divided into 

two regions: 1) the direct enumeration region; and 2) the measured-aliquot split sample 

region (see Quality Control - Sample Analysis). The Primary Taxonomist determines 

which taxa are abundant enough within a given sample to warrant enumeration after 

splitting, and uses a macro to construct a list of such taxa within region 2. All other taxa 

in the sample are added to a list in region 1 as they are encountered. The Primary 

Taxonomist processes the sample by methodically examining successive aliquots, 

directly enumerating the taxa in region 1 and ignoring the taxa in region 2. Each aliquot 

is rinsed into a 1000 ml graduated cylinder after examination and enumeration are 

complete. After all aliquots have been processed, the cylinder is covered and inverted six 

times and a 30-50 ml aliquot is quickly poured off. Beginning and ending volumes 

within the graduated cylinder are entered into specific cells within region 2, and a 

multiplier value is automatically calculated. Region 2 of the template is printed, 

complete with the multiplier value. The printout of region 2 is transferred to the 

Secondary Taxonomist along with the measured aliquot and the remainder of the sample, 

which has been rinsed from the graduated cylinder back into its original, labeled cod-end 

jar. The Secondary Taxonomist enumerates the listed taxa in the measured aliquot using 

a second zoom stereomicroscope. At the computer, the Primary Taxonomist examines 

the region 1 and region 2 lists for errors and then executes a macro that transposes the 

lists to the database region of the spreadsheet. Counts from the direct enumeration and 

Form LFD are included within this data transposition. After the Secondary Taxonomist 

has processed the measured aliquot, the measured aliquot is returned to its original codend 

jar and numbers estimated for region 2 taxa are entered into the database. All 

template areas are automatically cleared of data after use. 

8.8.5 Quality Control 

8.8.5.1 Sample Collection 

The collection vessel’s position is tracked by a GPS plotter, providing the QA Officer 

with a record of collection locations visited by the vessel during individual collection

Page 9 

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April 2008 


efforts. The recorded cruise track is inspected routinely. Flowmeter readings record 

inconsistencies in tow speed or tow duration. Filtered volumes are calculated after each 

collection effort, and any discrepancies in volumes filtered are investigated. In most 

cases, discrepancies are caused by reduction of the net’s filtration efficiency due to 

clogging by ctenophores, hydromedusae, diatoms, or larvacean houses. Any 

unexplainable discrepancy or undue variation in filtered volume is brought to the 

attention of the Field Operations Manager, who is responsible for future corrective action. 

8.8.5.2 Sample Analysis 

Very little has been published regarding the repeatability, accuracy and efficiency of 

different plankton enumeration methods. The characteristics of different methods were 

examined as part of the process of establishing standard analytical protocols. Three 

enumeration methods were evaluated using copepods and cumaceans as test subjects: 1) 

direct enumeration of the entire sample; 2) measured-aliquot subsampling of a repeatedly 

inverted sample; and 3) subsampling with a Motoda box. Five trials with each method 

produced the estimates in Table 8.2. 

The Motoda box is the most commonly used means of splitting plankton samples, but this 

method was found to have the poorest repeatability and accuracy. Before conducting the 

above experiment, the ability of the Motoda box to evenly split tap water samples 

(without organisms) was confirmed in five trials, in which the mean split was 49.7 vs. 

50.3% for the two sides of the box. When organisms were included, the accuracy of each 

split with the Motoda box depended on the homogeneity of the organism distribution 

prior to each splitting step. If the distribution was not homogeneous, then the associated 

error was quickly compounded with each additional split. This effect produced 

consistently high estimates in the case of the cumacean Cyclaspis. The circulation 

characteristics within the Motoda box do not guarantee a homogenous distribution of all 

taxa in the sample. The measured aliquot method appears to be superior to the Motoda 

box method because 1) the sample is actively homogenized through repeated inversion 

immediately prior to subsampling; and 2) the subsampling is completed in a single step, 

eliminating the error propagation problem. The measured aliquot method was selected as 

the standard method for enumerating abundant planktonic organisms (n>100, 

approximately). Direct enumeration is used for less abundant taxa, which typically 

constitute more than two-thirds of the taxa encountered in an individual plankton sample. 

Quality control for sample analysis is achieved by routine re-analysis of 5% of the 

samples. The fit (r²) of linear regressions of the log-transformed counts (the counts have 

a lognormal distribution) is used as a measure of repeatability, with agreement below 

95% being considered unacceptable. 

Taxonomic quality control is achieved through maintenance of a photographic reference 

collection and through collaboration with other researchers. The latter effort includes 

both visits to the EEL by recognized taxonomic experts and shipment of specimens to 

other laboratories for independent identification. In some cases, reference specimens are

Page 10 

Section 8.0 

April 2008 


supplied by collaborating laboratories in order to confirm organism identities. In the 

event that a taxonomic error is discovered, one of two corrective actions will be taken: 1) 

all relevant specimens will be reexamined and reclassified; or 2) taxonomic designations 

will be revised, either through simple substitution or through a reduction of taxonomic 

resolution (e.g., from species level to genus level). 

8.8.5.3 Instrument/Equipment Inspection and Maintenance 

Between deployments, the plankton nets and sieves are soaked in a detergent solution 

containing protease enzyme (Terg-A-Zyme) to remove deposits from the meshes. The 

net is inspected for mesh damage during each wash down (i.e., after each deployment). 

In the event of damage or loss of the primary collection gear, which consists of the net, 

flowmeter, weights, calibrated tow line, bridles and cod-end-jar, reserve collection gear 

of identical specifications is available for immediate substitution. The reserve collection 

gear is maintained in the same condition as the primary collection gear. During the latter 

part of each tow, the collection gear is visible from the boat and is routinely inspected to 

confirm correct deployment. 

8.8.5.4 Instrument/Equipment Calibration 

All instruments requiring calibration are uniquely identifiable to allow tracing of 

calibration records to individual instruments. These instruments consist of 1) water 

quality instruments; and 2) flowmeters designed to be deployed in the mouths of plankton 

nets. 

Water quality instruments are used to measure water temperature, specific conductance, 

pH and dissolved oxygen and are calibrated before and after deployment according to the 

manufacturer’s specifications. 

• A YSI 556 Multiprobe is the primary instrument used to make water quality 

measurements. 

• Reserve, or back-up, water quality instruments are other YSI 556s. 

The buffer solutions used to calibrate the pH sensor at 7.00 and 10.00 are traceable to 

N.I.S.T. 187C:186IF:186IIF, and are certified to be accurate to within ∀0.02 pH units at 

25°C. A conductivity standard reference solution (8,759 ppm KCl) of 15,000 µS 

conductivity at 25°C, traceable to N.I.S.T. Aqueous Electrolytic Conductivity Standard 

Reference Solution 3193, is used to standardize KCl reference solutions produced within 

the EEL. All reference standards are stored in tightly sealed containers and are not used 

after their expiration date. Standard solutions that have been used for calibration are 

retained in separate, labeled containers for one-time re-use as sensor rinse. Used 

calibration solutions are never returned to their original containers. Project-specific 

calibration forms are used to record calibration data and are kept in the EEL.

Page 11 

Section 8.0 

April 2008 


Individual mechanical flowmeters are numbered with permanent ink. New flowmeters 

are calibrated before initial deployment. The primary and reserve flowmeters are 

calibrated approximately every four months. If average error exceeds +/−5%, a new rotor 

constant is calculated and substituted for the manufacturer’s constant during filtered 

volume calculation. Error >10%, as calculated using the manufacturer’s rotor constant, 

results in discontinued use of the flowmeter until it can be repaired or replaced. 

Completed flowmeter calibration forms are kept in the EEL. 

8.8.5.5 Inspection/Acceptance of Supplies and Consumables 

After expiration of sample archival periods, plastic cod-end jars and lids may be cleaned 

in protease solution and re-used. The criteria for re-use of individual jars include 1) 

absence of all foreign material or deposits inside the jar and lid and 2) successful removal 

of any existing sample identifiers that would obscure the identity of the new sample. 

Surplus 50% 2-propanol solution left over after sample processing may be re-used after 

filtering through a 63 µm or finer sieve. Chemicals used to prepare standard solutions are 

A.C.S. certified and are accepted from the supplier only if the container’s seal is 

unbroken at the time of delivery. As described above, standard solutions prepared within 

the EEL are tested against N.I.S.T. traceable standards, further guaranteeing the precision 

of prepared standards. 

8.8.5.6 Data Management 

Standardized field data forms are completed in the field by the Field Operations Manager. 

Upon returning from the field, the Field Operations Manager reviews each form to 

confirm that all necessary data have been recorded. The forms are then placed in a threering 

binder in the EEL. 

The database manager enters the field data into a Lotus 1-2-3 spreadsheet that contains 

specialized macros and data entry templates. The data entry template for the water 

quality data simulates the appearance of the field data form. This facilitates comparison 

of data on the field data sheet with newly entered data. Once the data entry has been 

verified, the database manager executes a macro that transposes each column of the 

template grid to the next available row in the database region of the spreadsheet, 

automatically aligning the data within the proper columns. 

Six-digit flowmeter readings from the field data forms are also entered using a 

specialized template. The template contains a formula that calculates the filtered volume. 

The database manager examines each calculated volume before it is copied to the 

database region of the spreadsheet. This step flags errors in entry of the flowmeter 

readings. 

Taxonomic data are entered into the database directly from the microscope workstation 

(see Analytical Methods). All data are stored on a hard drive and are backed up onto 

either a Zip disk, flash drive or CD at the end of each sample processing session. The

Page 12 

Section 8.0 

April 2008 


database will be provided to PBS&J on a quarterly basis, providing further database 

back-up. 

8.9 Scope of Work 

The Subcontract for Professional Services and Scope of Work specifically address field 

sampling, sample processing, data management, quality control, and project management 

associated with the implementation of the Zooplankton and Fish Monitoring Components 

of the final HBMP design, as described in the HBMP design document (PBS&J, 2000). 

The Subconsultant - University of South Florida, College of Marine Science, Dr. Ernst 

Peebles (with the Florida Marine Resources Institute serving as a Subcontractor) shall 

perform the following professional services under this Agreement. 

8.9.1 QA/QC Plan Preparation 

The Subconsultant shall prepare a project-specific QA/QC Plan describing all quality 

control and quality assurance procedures to be conducted throughout the implementation 

of the Zooplankton and Fish Monitoring Components of the HBMP. The QA/QC Plan 

will address the overall project approach and rationale, and specify quality control and 

quality assurance protocols and procedures, appropriate to each monitoring component. 

The format for the QA/QC Plan shall generally be as described in Chapter 8 of the final 

HBMP design document. Detailed specifications for the adult and juvenile fishes HBMP 

monitoring element are provided in Section 7 of this QA/QC Plan. 

8.9.2 Zooplankton Sample Collection and Processing 

This task will entail the collection of zooplankton samples, and the laboratory processing 

and analysis of those samples for species composition and abundance of ichthyoplankton 

and invertebrate zooplankton, including vertically migrating epibenthos. Sample 

collection, processing and analysis will be conducted throughout the 5-year study period. 

The total number of samples, as well as the spatial and temporal stratification of the 

sample events in each reporting unit is defined in Table 8.3. PBS&J shall be responsible 

for providing to the Subconsultant a sampling schedule indicating when and where to 

sample in each of the reporting units. 

The Subconsultant shall conduct field sample collection and laboratory processing of 

biological samples following the protocols employed by the University of South Florida 

College of Marine Science, as conducted for SWFWMD on similar studies. 

Ichthyoplankton and other zooplankton will be sampled using a 0.5 meter mouth 

diameter, 500 µm mesh, conical (3:1) plankton net. All other specifications and 

protocols for the Zooplankton Monitoring Component shall be as generally described in 

the HBMP design document, and as detailed in the project specific QA/QC Plan.

Page 13 

Section 8.0 

April 2008 


8.9.3 Data Management 

The Subconsultant shall be responsible for the proper management of all data generated 

from the Zooplankton and Fish Monitoring Components of the HBMP, and shall develop 

and implement a rigorous data management process throughout the entire study period. 

Data management includes data collection, handling, evaluation, verification, validation, 

and reporting activities. 

The Subconsultant shall use Microsoft Access, a fully relational database that is readily 

available to many users, to develop the HBMP database. Export of data from Access to 

other software applications such as Excel (or other spreadsheets), SAS, and HTML for 

Internet applications will be facilitated by this approach. The use of a relational database 

will ensure that many different data types can be effectively and correctly linked using 

common variable names and values. 

A Data Dictionary shall be developed and annually updated by the Subconsultant to 

ensure that all database users are kept fully abreast of the database contents and their 

applications. The primary data collected in either the field or in the laboratory will be 

copied, either in hard copy or digital format, and provided to the designated Data 

Manager as identified in the project specific QA/QC Plan. Hard copy data, after a visual 

form check, will be entered into the computer database in an appropriate manner to 

assure data quality goals are satisfied. The resulting data set will be stored and provided 

with a unique file name identifier, which contains all of the information necessary for 

linking data spatially and temporally. This data set will be the raw data set. Only the 

project Data Manager and their designees will have read/write abilities for this and any 

future data set resulting from this data collection effort. All other database users will be 

provided with read-only access to the database. The original versions of all raw data sets 

will be archived. 

Following storage of the raw data set, error and range checking procedures for data 

verification purposes will be performed. The resulting data set will again be provided a 

unique file name identifier and will be stored as the verified data set for a given HBMP 

program element, year, and month. Cross comparison of verified data sets from the 

different HBMP elements will allow further validation of the data sets. Final review of 

the data in the verified data sets will also include any other cross comparisons, for 

example, between field and laboratory data, or between taxonomic experts. The resulting 

validated data set will be stored, given a unique file name, and provided to PBS&J for 

inclusion in the master HBMP database. 

8.9.4 Project Management 

The Subconsultant shall provide the necessary project management activities necessary to 

ensure the successful completion of all tasks described in this Scope of Work in a timely 

and efficient manner. The Subconsultant will collect the financial and management data

Page 14 

Section 8.0 

April 2008 


necessary for evaluating work progress compared to the requirements of this agreement 

for the duration of the contract. 

8.9.5 Meetings 

The Subconsultant shall attend and participate in the annual meeting of the HBMP Focus 

Group at the end of each full calendar year covered under this Agreement. Specifically, 

the Subconsultant shall prepare and present to the Focus Group an annual summary of the 

data collected under the Zooplankton and Fish Monitoring Components of the HBMP. 

The purpose of the Focus Group meetings will be to review the HBMP data generated 

during the current year, to evaluate the effectiveness of the HBMP design, and to make 

recommendations with regard to modifications to the sampling design or to the data 

analysis procedures. 

The Subconsultant may be asked to attend and participate in other project related 

meetings, on an as needed basis throughout the duration of this Agreement. The 

Subconsultant shall be reimbursed on a time and materials basis for attendance and 

participation in meetings other than the annual Focus Group meetings.

Page 15 

Section 8.0 

April 2008 


Table 8.1 Form LFD 

Sample Number __________Form ____ of ____ Beaker No. ________ 

Taxon Stage Number 

Sample Number __________Form ____ of ____ 

Beaker No. ________ 

Taxon Stage Number

Page 16 

Section 8.0 

April 2008 


Table 8.2 Estimates of Organism Number using Three Methods, Presented as the 

Mean and Standard Deviation of Five Independent Trials 

Direct Enumeration 

Measured Aliquot 

Motoda Box 

Taxa 

(All Specimens 

Counted) 

(12-17% Volumetric 

Subsample 

Counted) 

(12.5% Volumetric 

Subsample 

Counted) 

Acartia tonsa 

(copepod) 

Error! = 1,334 

s.d. = 157 

x = 1,379 

s.d. = 179 

x = 1,290 

s.d. = 214 

Cyclaspis sp. 

(cumacean) 

x = 15,077 

s.d. = 1,908 

x = 15,387 

s.d. = 1,655 

x = 21,747 

s.d. = 3,307

Page 17 

Section 8.0 

April 2008 


Table 8.3 Summary of Ichthyoplankton and Zooplankton HBMP Sampling Elements 

Sampling Program Summary For The lower Hillsborough River. 

Element # Strata Jan Feb March April May June July August September October November December Total 





stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

144 

hauls 

Sampling program summary for the lower Alafia River 





stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

144 

hauls 

Sampling program summary for McKay Bay 




6 cells 

shallow 

2 strata - 

channel 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 hauls/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

1 haul/ 

cell 

2 hauls/ 

stratum 

72 

hauls 

48 

hauls 

Sampling program summary for the lower Palm River. 




3 strata - 

channel 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

2 hauls/ 

stratum 

72 

hauls

Page 18 

Section 8.0 

April 2008 


Page 19 

Section 8.0 

April 2008 


Figure 8.2. Pairing of beakers and labeled cod-end jars in staging area. Small fraction 

identity is also maintained by Form LFD.

Appendix 8-A 


Updated for data through January, 2007


Taxcode PhyloOrder Description Common Name 

12100 516 Lepisosteus sp. eggs gar 

12101 517 Lepisosteus sp. preflexion larvae gar 

12102 518 Lepisosteus sp. flexion larvae gar 

12103 519 Lepisosteus sp. postflexion larvae gar 

12104 520 Lepisosteus sp. juveniles gar 

12110 510 Lepisosteus platyrhincus eggs Florida gar 

12111 511 

Lepisosteus platyrhincus preflexion 

larvae 

Florida gar 

12112 512 

Lepisosteus platyrhincus flexion 

larvae 

Florida gar 

12113 513 

Lepisosteus platyrhincus postflexion 

larvae 

Florida gar 

12114 514 Lepisosteus platyrhincus juveniles Florida gar 

12115 515 Lepisosteus platyrhincus adults Florida gar 

12120 504 Lepisosteus osseus eggs longnose gar 

12121 505 Lepisosteus osseus preflexion larvae longnose gar 

12122 506 Lepisosteus osseus flexion larvae longnose gar 

12123 507 Lepisosteus osseus postflexion larvae longnose gar 

12124 508 Lepisosteus osseus juveniles longnose gar 

12125 509 Lepisosteus osseus adults longnose gar 

14110 524 Elops saurus eggs ladyfish 

14111 525 Elops saurus preflexion larvae ladyfish 

14112 526 Elops saurus flexion larvae ladyfish 

14113 527 Elops saurus postflexion larvae ladyfish 

14113.5 528 Elops saurus metamorphs ladyfish 

14114 529 Elops saurus juveniles ladyfish 

14115 530 Elops saurus adults ladyfish 

15113 521 Albula vulpes postflexion larvae bonefish 

15114 522 Albula vulpes juveniles bonefish 

15115 523 Albula vulpes adults bonefish 

16114 531 Anguilla rostrata juveniles American eel 

18210 532 Myrophis punctatus eggs speckled worm eel 

18211 533 Myrophis punctatus preflexion larvae speckled worm eel 

18212 534 Myrophis punctatus flexion larvae speckled worm eel 

18213 535 Myrophis punctatus postflexion larvae speckled worm eel 

18213.5 536 Myrophic punctatus metamorphs speckled worm eel 

18214 537 Myrophis punctatus juveniles speckled worm eel 

18215 538 Myrophis punctatus adults speckled worm eel 

19000 563 clupeid eggs herrings 

19001 564 clupeid preflexion larvae herrings 

19002 565 clupeid flexion larvae herrings 

19003 566 clupeid postflexion larvae herrings 

19100 579 Brevoortia spp. eggs menhaden



19101 580 Brevoortia spp. preflexion larvae menhaden 

19102 581 Brevoortia spp. flexion larvae menhaden 

19103 582 Brevoortia spp. postflexion larvae menhaden 

19103.5 583 Brevoortia spp. metamorphs menhaden 

19104 584 Brevoortia spp. juveniles menhaden 

19105 585 Brevoortia spp. adults menhaden 

19110 567 Brevoortia patronus eggs gulf menhaden 

19111 568 Brevoortia patronus preflexion larvae gulf menhaden 

19112 569 Brevoortia patronus flexion larvae gulf menhaden 

19113 570 Brevoortia patronus postflexion larvae gulf menhaden 

19114 571 Brevoortia patronus juveniles gulf menhaden 

19115 572 Brevoortia patronus adults gulf menhaden 

19120 573 Brevoortia smithi eggs gulf menhaden 

19121 574 Brevoortia smithi preflexion larvae yellowfin menhaden 

19122 575 Brevoortia smithi flexion larvae yellowfin menhaden 

19123 576 Brevoortia smithi postflexion larvae yellowfin menhaden 

19124 577 Brevoortia smithi juveniles yellowfin menhaden 

19125 578 Brevoortia smithi adults yellowfin menhaden 

19200 592 Dorosoma spp. eggs shads 

19201 593 Dorosoma spp. preflexion larvae shads 

19202 594 Dorosoma spp. flexion larvae shads 

19203 595 Dorosoma spp. postflexion larvae shads 

19203.5 596 Dorosoma spp. metamorphs shads 

19213.5 589 Dorosoma petenense metamorphs threadfin shad 

19214 590 Dorosoma petenense juveniles threadfin shad 

19215 591 Dorosoma petenense adults threadfin shad 

19223.5 586 Dorosoma cepedianum metamorphs gizzard shad 

19224 587 Dorosoma cepedianum juveniles gizzard shad 

19225 588 Dorosoma cepedianum adults gizzard shad 

19310 597 Harengula jaguana eggs scaled sardine 

19311 598 Harengula jaguana preflexion larvae scaled sardine 

19312 599 Harengula jaguana flexion larvae scaled sardine 

19313 600 Harengula jaguana postflexion larvae scaled sardine 

19313.5 601 Harengula jaguana metamorphs scaled sardine 

19314 602 Harengula jaguana juveniles scaled sardine 

19315 603 Harengula jaguana adults scaled sardine 

19410 604 Opisthonema oglinum eggs Atlantic thread herring 

19411 605 

Opisthonema oglinum preflexion 

larvae 

Atlantic thread herring 

19412 606 Opisthonema oglinum flexion larvae Atlantic thread herring 

19413 607 

Opisthonema oglinum postflexion 

larvae 

Atlantic thread herring 

19413.5 608 Opisthonema oglinum metamorphs Atlantic thread herring 

19414 609 Opisthonema oglinum juveniles Atlantic thread herring



19415 610 Opisthonema oglinum adults Atlantic thread herring 

19510 611 Sardinella aurita eggs Spanish sardine 

19511 612 Sardinella aurita preflexion larvae Spanish sardine 

19512 613 Sardinella aurita flexion larvae Spanish sardine 

19513 614 Sardinella aurita postflexion larvae Spanish sardine 

19513.5 615 Sardinella aurita metamorphs Spanish sardine 

19514 616 Sardinella aurita juveniles Spanish sardine 

19515 617 Sardinella aurita adults Spanish sardine 

20100 558 Anchoa spp. eggs anchovies 

20101 559 Anchoa spp. preflexion larvae anchovies 

20102 560 Anchoa spp. flexion larvae anchovies 

20103 561 Anchoa spp. postflexion larvae anchovies 

20104 562 Anchoa spp. juveniles anchovies 

20105 557 Anchoa spp. adults anchovies 

20110 545 Anchoa hepsetus eggs striped anchovy 

20111 546 Anchoa hepsetus preflexion larvae striped anchovy 

20112 547 Anchoa hepsetus flexion larvae striped anchovy 

20113 548 Anchoa hepsetus postflexion larvae striped anchovy 

20114 549 Anchoa hepsetus juveniles striped anchovy 

20115 550 Anchoa hepsetus adults striped anchovy 

20120 551 Anchoa mitchilli eggs bay anchovy 

20121 552 Anchoa mitchilli preflexion larvae bay anchovy 

20122 553 Anchoa mitchilli flexion larvae bay anchovy 

20123 554 Anchoa mitchilli postflexion larvae bay anchovy 

20124 555 Anchoa mitchilli juveniles bay anchovy 

20125 556 Anchoa mitchilli adults bay anchovy 

20130 539 Anchoa cubana eggs Cuban anchovy 

20131 540 Anchoa cubana preflexion larvae Cuban anchovy 

20132 541 Anchoa cubana flexion larvae Cuban anchovy 

20133 542 Anchoa cubana postflexion larvae Cuban anchovy 

20134 543 Anchoa cubana juveniles Cuban anchovy 

20135 544 Anchoa cubana adults Cuban anchovy 

21110 721 Synodus foetens eggs inshore lizardfish 

21111 722 Synodus foetens preflexion larvae inshore lizardfish 

21112 723 Synodus foetens flexion larvae inshore lizardfish 

21113 724 Synodus foetens postflexion larvae inshore lizardfish 

21114 725 Synodus foetens juveniles inshore lizardfish 

21115 726 Synodus foetens adults inshore lizardfish 

22110 684 Ariopsis felis eggs hardhead catfish 

22111 685 Ariopsis felis preflexion larvae hardhead catfish 

22112 686 Ariopsis felis flexion larvae hardhead catfish 

22113 687 Ariopsis felis postflexion larvae hardhead catfish 

22114 688 Ariopsis felis juveniles hardhead catfish



22115 689 Ariopsis felis adults hardhead catfish 

22210 696 Bagre marinus eggs gafftopsail sea catfish 

22211 697 Bagre marinus preflexion larvae gafftopsail sea catfish 

22212 698 Bagre marinus flexion larvae gafftopsail sea catfish 

22213 699 Bagre marinus postflexion larvae gafftopsail sea catfish 

22214 700 Bagre marinus juveniles gafftopsail sea catfish 

22215 701 Bagre marinus adults gafftopsail sea catfish 

23110 733 Opsanus beta eggs gulf toadfish 

23111 734 Opsanus beta preflexion larvae gulf toadfish 

23112 735 Opsanus beta flexion larvae gulf toadfish 

23113 736 Opsanus beta postflexion larvae gulf toadfish 

23114 737 Opsanus beta juveniles gulf toadfish 

23115 738 Opsanus beta adults gulf toadfish 

24110 1177 Gobiesox strumosus eggs skilletfish 

24111 1178 Gobiesox strumosus preflexion larvae skilletfish 

24112 1179 Gobiesox strumosus flexion larvae skilletfish 

24113 1180 Gobiesox strumosus postflexion larvae skilletfish 

24114 1181 Gobiesox strumosus juveniles skilletfish 

24115 1182 Gobiesox strumosus adults skilletfish 

27110 727 Urophycis floridana eggs southern hake 

27111 728 Urophycis floridana preflexion larvae southern hake 

27112 729 Urophycis floridana flexion larvae southern hake 

27113 730 Urophycis floridana postflexion larvae southern hake 

27114 731 Urophycis floridana juveniles southern hake 

27115 732 Urophycis floridana adults southern hake 

29210 808 Hyporhamphus unifasciatus eggs silverstripe halfbeak 

29211 809 

Hyporhamphus unifasciatus preflexion 

larvae 

silverstripe halfbeak 

29212 810 

Hyporhamphus unifasciatus flexion 

larvae 


29213 811 

Hyporhamphus unifasciatus 

postflexion larvae 


29214 812 Hyporhamphus unifasciatus juveniles silverstripe halfbeak 

29215 813 Hyporhamphus unifasciatus adults silverstripe halfbeak 

30100 790 Strongylura spp. eggs needlefishes 

30101 791 Strongylura spp. preflexion larvae needlefishes 

30102 792 Strongylura spp. flexion larvae needlefishes 

30103 793 Strongylura spp. postflexion larvae needlefishes 

30104 794 Strongylura spp. juveniles needlefishes 

30105 795 Strongylura spp. adults needlefishes 

30110 778 Strongylura marina eggs Atlantic needlefish 

30111 779 Strongylura marina preflexion larvae Atlantic needlefish 

30112 780 Strongylura marina flexion larvae Atlantic needlefish 

30113 781 Strongylura marina postflexion larvae Atlantic needlefish



30114 782 Strongylura marina juveniles Atlantic needlefish 

30115 783 Strongylura marina adults Atlantic needlefish 

30120 784 Strongylura notata eggs redfin needlefish 

30121 785 Strongylura notata preflexion larvae redfin needlefish 

30122 786 Strongylura notata flexion larvae redfin needlefish 

30123 787 Strongylura notata postflexion larvae redfin needlefish 

30124 788 Strongylura notata juveniles redfin needlefish 

30125 789 Strongylura notata adults redfin needlefish 

30200 796 Tylosurus acus eggs agujon 

30201 797 Tylosurus acus preflexion larvae agujon 

30202 798 Tylosurus acus flexion larvae agujon 

30203 799 Tylosurus acus postflexion larvae agujon 

30204 800 Tylosurus acus juveniles agujon 

30205 801 Tylosurus acus adults agujon 

31210 858 Cyprinodon variegatus eggs sheepshead minnow 

31211 859 

Cyprinodon variegatus preflexion 

larvae 

sheepshead minnow 

31212 860 Cyprinodon variegatus flexion larvae sheepshead minnow 

31213 861 

Cyprinodon variegatus postflexion 

larvae 

sheepshead minnow 

31214 862 Cyprinodon variegatus juveniles sheepshead minnow 

31215 863 Cyprinodon variegatus adults sheepshead minnow 

31400 832 Fundulus spp. eggs killifishes 

31401 833 Fundulus spp. preflexion larvae killifishes 

31402 834 Fundulus spp. flexion larvae killifishes 

31403 835 Fundulus spp. postflexion larvae killifishes 

31404 836 Fundulus spp. juveniles killifishes 

31405 837 Fundulus spp. adults killifishes 

31410 826 Fundulus seminolis eggs Seminole killifish 

31411 827 Fundulus seminolis preflexion larvae Seminole killifish 

31412 828 Fundulus seminolis flexion larvae Seminole killifish 

31413 829 Fundulus seminolis postflexion larvae Seminole killifish 

31414 830 Fundulus seminolis juveniles Seminole killifish 

31415 831 Fundulus seminolis adults Seminole killifish 

31420 814 Fundulus grandis eggs gulf killifish 

31421 815 Fundulus grandis preflexion larvae gulf killifish 

31422 816 Fundulus grandis flexion larvae gulf killifish 

31423 817 Fundulus grandis postflexion larvae gulf killifish 

31424 818 Fundulus grandis juveniles gulf killifish 

31425 819 Fundulus grandis adults gulf killifish 

31430 820 Fundulus majalis eggs striped killifish 

31431 821 Fundulus majalis preflexion larvae striped killifish 

31432 822 Fundulus majalis flexion larvae striped killifish 

31433 823 Fundulus majalis postflexion larvae striped killifish



31434 824 Fundulus majalis juveniles striped killifish 

31435 825 Fundulus majalis adults striped killifish 

31510 844 Lucania parva eggs rainwater killifish 

31511 845 Lucania parva preflexion larvae rainwater killifish 

31512 846 Lucania parva flexion larvae rainwater killifish 

31513 847 Lucania parva postflexion larvae rainwater killifish 

31514 848 Lucania parva juveniles rainwater killifish 

31515 849 Lucania parva adults rainwater killifish 

31520 838 Lucania goodei eggs bluefin killifish 

31521 839 Lucania goodei preflexion larvae bluefin killifish 

31522 840 Lucania goodei flexion larvae bluefin killifish 

31523 841 Lucania goodei postflexion larvae bluefin killifish 

31524 842 Lucania goodei juveniles bluefin killifish 

31525 843 Lucania goodei adults bluefin killifish 

31610 802 Rivulus marmoratus eggs mangrove rivulus 

31611 803 Rivulus marmoratus preflexion larvae mangrove rivulus 

31612 804 Rivulus marmoratus flexion larvae mangrove rivulus 

31613 805 Rivulus marmoratus postflexion larvae mangrove rivulus 

31614 806 Rivulus marmoratus juveniles mangrove rivulus 

31615 807 Rivulus marmoratus adults mangrove rivulus 

31710 864 Jordanella floridae eggs flagfish 

31711 865 Jordanella floridae preflexion larvae flagfish 

31712 866 Jordanella floridae flexion larvae flagfish 

31713 867 Jordanella floridae postflexion larvae flagfish 

31714 868 Jordanella floridae juveniles flagfish 

31715 869 Jordanella floridae adults flagfish 

32114 852 Gambusia holbrooki juveniles eastern mosquitofish 

32115 853 Gambusia holbrooki adults eastern mosquitofish 

32214 854 Heterandria formosa juveniles least killifish 

32215 855 Heterandria formosa adults least killifish 

32314 856 Poecilia latipinna juveniles sailfin molly 

32315 857 Poecilia latipinna adults sailfin molly 

32414 850 Belonesox belizanus juveniles pike killifish 

32415 851 Belonesox belizanus adults pike killifish 

33000 1360 fish eggs, atherinomorph silversides 

33100 772 Menidia spp. eggs silversides 

33101 773 Menidia spp. preflexion larvae silversides 

33102 774 Menidia spp. flexion larvae silversides 

33103 775 Menidia spp. postflexion larvae silversides 

33104 776 Menidia spp. juveniles silversides 

33105 777 Menidia spp. adults silversides 

33110 769 Menidia beryllina eggs inland silverside 

33114 770 Menidia beryllina juveniles inland silverside



33115 771 Menidia beryllina adults inland silverside 

33210 763 Membras martinica eggs rough silverside 

33211 764 Membras martinica preflexion larvae rough silverside 

33212 765 Membras martinica flexion larvae rough silverside 

33213 766 Membras martinica postflexion larvae rough silverside 

33214 767 Membras martinica juveniles rough silverside 

33215 768 Membras martinica adults rough silverside 

33310 757 Labidesthes sicculus eggs brook silverside 

33311 758 Labidesthes sicculus preflexion larvae brook silverside 

33312 759 Labidesthes sicculus flexion larvae brook silverside 

33313 760 

Labidesthes sicculus postflexion 

larvae 

brook silverside 

33314 761 Labidesthes sicculus juveniles brook silverside 

33315 762 Labidesthes sicculus adults brook silverside 

34114 870 Hippocampus erectus juveniles lined seahorse 

34115 871 Hippocampus erectus adults lined seahorse 

34124 872 Hippocampus zosterae juveniles dwarf seahorse 

34125 873 Hippocampus zosterae adults dwarf seahorse 

34214 874 Syngnathus floridae juveniles dusky pipefish 

34215 875 Syngnathus floridae adults dusky pipefish 

34224 876 Syngnathus louisianae juveniles chain pipefish 

34225 877 Syngnathus louisianae adults chain pipefish 

34234 878 Syngnathus scovelli juveniles gulf pipefish 

34235 879 Syngnathus scovelli adults gulf pipefish 

35110 900 Centropomus undecimalis eggs snook 

35111 901 

Centropomus undecimalis preflexion 

larvae 

snook 

35112 902 

Centropomus undecimalis flexion 

larvae 

snook 

35113 903 

Centropomus undecimalis postflexion 

larvae 

snook 

35114 904 Centropomus undecimalis juveniles snook 

35115 905 Centropomus undecimalis adults snook 

37230 906 Epinephelus itajara eggs goliath grouper 

37231 907 Epinephelus itajara preflexion larvae goliath grouper 

37232 908 Epinephelus itajara flexion larvae goliath grouper 

37233 909 Epinephelus itajara postflexion larvae goliath grouper 

37234 910 Epinephelus itajara juveniles goliath grouper 

37235 911 Epinephelus itajara adults goliath grouper 

43210 981 Chloroscombrus chrysurus eggs Atlantic bumper 

43211 982 

Chloroscombrus chrysurus preflexion 

larvae 

Atlantic bumper 

43212 983 

Chloroscombrus chrysurus flexion 

larvae 

Atlantic bumper



43213 984 

Chloroscombrus chrysurus postflexion 

larvae 

Atlantic bumper 

43214 985 Chloroscombrus chrysurus juveniles Atlantic bumper 

43215 986 Chloroscombrus chrysurus adults Atlantic bumper 

43410 987 Hemicaranx amblyrhynchus eggs bluntnose jack 

43411 988 

Hemicaranx amblyrhynchus 

preflexion larvae 

bluntnose jack 

43412 989 

Hemicaranx amblyrhynchus flexion 

larvae 


43413 990 

Hemicaranx amblyrhynchus 



43414 991 Hemicaranx amblyrhynchus juveniles bluntnose jack 

43415 992 Hemicaranx amblyrhynchus adults bluntnose jack 

43510 993 Oligoplites saurus eggs leatherjack 

43511 994 Oligoplites saurus preflexion larvae leatherjack 

43512 995 Oligoplites saurus flexion larvae leatherjack 

43513 996 Oligoplites saurus postflexion larvae leatherjack 

43514 997 Oligoplites saurus juveniles leatherjack 

43515 998 Oligoplites saurus adults leatherjack 

43810 999 Uraspis secunda eggs cottonmouth jack 

43811 1000 Uraspis secunda preflexion larvae cottonmouth jack 

43812 1001 Uraspis secunda flexion larvae cottonmouth jack 

43813 1002 Uraspis secunda postflexion larvae cottonmouth jack 

43814 1003 Uraspis secunda juveniles cottonmouth jack 

43815 1004 Uraspis secunda adults cottonmouth jack 

44120 1005 Lutjanus griseus eggs gray snapper 

44121 1006 Lutjanus griseus preflexion larvae gray snapper 

44122 1007 Lutjanus griseus flexion larvae gray snapper 

44123 1008 Lutjanus griseus postflexion larvae gray snapper 

44124 1009 Lutjanus griseus juveniles gray snapper 

44125 1010 Lutjanus griseus adults gray snapper 

46000 1029 gerreid eggs mojjaras 

46001 1030 gerreid preflexion larvae mojjaras 

46002 1031 gerreid flexion larvae mojjaras 

46003 1032 gerreid postflexion larvae mojjaras 

46004 1033 gerreid juveniles mojjaras 

46100 1034 Eugerres plumieri eggs striped mojarra 

46101 1035 Eugerres plumieri preflexion larvae striped mojarra 

46102 1036 Eugerres plumieri flexion larvae striped mojarra 

46103 1037 Eugerres plumieri postflexion larvae striped mojarra 

46104 1038 Eugerres plumieri juveniles striped mojarra 

46105 1039 Eugerres plumieri adults striped mojarra 

46200 1023 Eucinostomus spp. eggs mojarras 

46201 1024 Eucinostomus spp. preflexion larvae mojarras



46202 1025 Eucinostomus spp. flexion larvae mojarras 

46203 1026 Eucinostomus spp. postflexion larvae mojarras 

46204 1027 Eucinostomus spp. juveniles mojarras 

46205 1028 Eucinostomus spp. adults mojarras 

46210 1011 Eucinostomus gula eggs silver jenny 

46211 1012 Eucinostomus gula preflexion larvae silver jenny 

46212 1013 Eucinostomus gula flexion larvae silver jenny 

46213 1014 Eucinostomus gula postflexion larvae silver jenny 

46214 1015 Eucinostomus gula juveniles silver jenny 

46215 1016 Eucinostomus gula adults silver jenny 

46220 1017 Eucinostomus harengulus eggs tidewater mojarra 

46221 1018 

Eucinostomus harengulus preflexion 

larvae 

tidewater mojarra 

46222 1019 

Eucinostomus harengulus flexion 

larvae 


46223 1020 

Eucinostomus harengulus postflexion 

larvae 


46224 1021 Eucinostomus harengulus juveniles tidewater mojarra 

46225 1022 Eucinostomus harengulus adults tidewater mojarra 

47110 1040 Haemulon plumieri eggs white grunt 

47111 1041 Haemulon plumieri preflexion larvae white grunt 

47112 1042 Haemulon plumieri flexion larvae white grunt 

47113 1043 Haemulon plumieri postflexion larvae white grunt 

47114 1044 Haemulon plumieri juveniles white grunt 

47115 1045 Haemulon plumieri adults white grunt 

47210 1054 Orthopristis chrysoptera eggs pigfish 

47211 1055 

Orthopristis chrysoptera preflexion 

larvae 

pigfish 

47212 1056 Orthopristis chrysoptera flexion larvae pigfish 

47213 1057 

Orthopristis chrysoptera postflexion 

larvae 

pigfish 

47214 1058 Orthopristis chrysoptera juveniles pigfish 

47215 1059 Orthopristis chrysoptera adults pigfish 

48110 1046 Archosargus probatocephalus eggs sheepshead 

48111 1047 

Archosargus probatocephalus 


sheepshead 

48112 1048 

Archosargus probatocephalus flexion 

larvae 

sheepshead 

48113 1049 



sheepshead 

48114 1050 


juveniles 

sheepshead 

48115 1051 Archosargus probatocephalus adults sheepshead 

48410 1066 Lagodon rhomboides eggs pinfish



48411 1067 Lagodon rhomboides preflexion larvae pinfish 

48412 1068 Lagodon rhomboides flexion larvae pinfish 

48413 1069 

Lagodon rhomboides postflexion 

larvae 

pinfish 

48414 1070 Lagodon rhomboides juveniles pinfish 

48415 1071 Lagodon rhomboides adults pinfish 

49000 1072 sciaenid eggs drums and seatrout 

49110 1060 Bairdiella chrysoura eggs silver perch 

49111 1061 Bairdiella chrysoura preflexion larvae silver perch 

49112 1062 Bairdiella chrysoura flexion larvae silver perch 

49113 1063 Bairdiella chrysoura postflexion larvae silver perch 

49114 1064 Bairdiella chrysoura juveniles silver perch 

49115 1065 Bairdiella chrysoura adults silver perch 

49210 1073 Cynoscion arenarius eggs sand seatrout 

49211 1074 Cynoscion arenarius preflexion larvae sand seatrout 

49212 1075 Cynoscion arenarius flexion larvae sand seatrout 

49213 1076 

Cynoscion arenarius postflexion 

larvae 

sand seatrout 

49214 1077 Cynoscion arenarius juveniles sand seatrout 

49215 1078 Cynoscion arenarius adults sand seatrout 

49220 1079 Cynoscion nebulosus eggs spotted seatrout 

49221 1080 Cynoscion nebulosus preflexion larvae spotted seatrout 

49222 1081 Cynoscion nebulosus flexion larvae spotted seatrout 

49223 1082 

Cynoscion nebulosus postflexion 

larvae 

spotted seatrout 

49224 1083 Cynoscion nebulosus juveniles spotted seatrout 

49225 1084 Cynoscion nebulosus adults spotted seatrout 

49410 1085 Leiostomus xanthurus eggs spot 

49411 1086 

Leiostomus xanthurus preflexion 

larvae 

spot 

49412 1087 Leiostomus xanthurus flexion larvae spot 

49413 1088 

Leiostomus xanthurus postflexion 

larvae 

spot 

49414 1089 Leiostomus xanthurus juveniles spot 

49415 1090 Leiostomus xanthurus adults spot 

49500 1097 Menticirrhus spp. eggs kingfishes 

49501 1098 Menticirrhus spp. preflexion larvae kingfishes 

49502 1099 Menticirrhus spp. flexion larvae kingfishes 

49503 1100 Menticirrhus spp. postflexion larvae kingfishes 

49504 1101 Menticirrhus spp. juveniles kingfishes 

49505 1102 Menticirrhus spp. adults kingfishes 

49520 1091 Menticirrhus americanus eggs southern kingfish 

49521 1092 

Menticirrhus americanus preflexion 

larvae 

southern kingfish



49522 1093 

Menticirrhus americanus flexion 

larvae 

southern kingfish 

49523 1094 

Menticirrhus americanus postflexion 

larvae 

southern kingfish 

49524 1095 Menticirrhus americanus juveniles southern kingfish 

49525 1096 Menticirrhus americanus adults southern kingfish 

49610 1106 Micropogonias undulatus eggs Atlantic croaker 

49611 1107 

Micropogonias undulatus preflexion 

larvae 

Atlantic croaker 

49612 1108 

Micropogonias undulatus flexion 

larvae 


49613 1109 

Micropogonias undulatus postflexion 

larvae 


49614 1110 Micropogonias undulatus juveniles Atlantic croaker 

49615 1111 Micropogonias undulatus adults Atlantic croaker 

49710 1112 Pogonias cromis eggs black drum 

49711 1113 Pogonias cromis preflexion larvae black drum 

49712 1114 Pogonias cromis flexion larvae black drum 

49713 1115 Pogonias cromis postflexion larvae black drum 

49714 1116 Pogonias cromis juveniles black drum 

49715 1117 Pogonias cromis adults black drum 

49810 1118 Sciaenops ocellatus eggs red drum 

49811 1119 Sciaenops ocellatus preflexion larvae red drum 

49812 1120 Sciaenops ocellatus flexion larvae red drum 

49813 1121 Sciaenops ocellatus postflexion larvae red drum 

49814 1122 Sciaenops ocellatus juveniles red drum 

49815 1123 Sciaenops ocellatus adults red drum 

49901 1105 

unident. sciaenid (Equetus) preflexion 

larvae 

drum 

49902 1103 

unident. sciaenid (Equetus) flexion 

larvae 

drum 

49903 1104 

unident. sciaenid (Equetus) 


drum 

51110 1277 Chaetodipterus faber eggs Atlantic spadefish 

51111 1278 Chaetodipterus faber preflexion larvae Atlantic spadefish 

51112 1279 Chaetodipterus faber flexion larvae Atlantic spadefish 

51113 1280 

Chaetodipterus faber postflexion 

larvae 

Atlantic spadefish 

51114 1281 Chaetodipterus faber juveniles Atlantic spadefish 

51115 1282 Chaetodipterus faber adults Atlantic spadefish 

56110 739 Mugil cephalus eggs striped mullet 

56111 740 Mugil cephalus preflexion larvae striped mullet 

56112 741 Mugil cephalus flexion larvae striped mullet 

56113 742 Mugil cephalus postflexion larvae striped mullet



56114 743 Mugil cephalus juveniles striped mullet 

56115 744 Mugil cephalus adults striped mullet 

56120 745 Mugil curema eggs white mullet 

56121 746 Mugil curema preflexion larvae white mullet 

56122 747 Mugil curema flexion larvae white mullet 

56123 748 Mugil curema postflexion larvae white mullet 

56124 749 Mugil curema juveniles white mullet 

56125 750 Mugil curema adults white mullet 

56130 751 Mugil gyrans eggs fantail mullet 

56131 752 Mugil gyrans preflexion larvae fantail mullet 

56132 753 Mugil gyrans flexion larvae fantail mullet 

56133 754 Mugil gyrans postflexion larvae fantail mullet 

56134 755 Mugil gyrans juveniles fantail mullet 

56135 756 Mugil gyrans adults fantail mullet 

60110 1148 Astroscopus y-graecum eggs southern stargazer 

60111 1149 

Astroscopus y-graecum preflexion 

larvae 

southern stargazer 

60112 1150 Astroscopus y-graecum flexion larvae southern stargazer 

60113 1151 

Astroscopus y-graecum postflexion 

larvae 

southern stargazer 

60114 1152 Astroscopus y-graecum juveniles southern stargazer 

60115 1153 Astroscopus y-graecum adults southern stargazer 

61000 1160 clinid eggs clinids 

61001 1161 clinid prefelxion larvae clinids 

61002 1162 clinid flexion larvae clinids 

61003 1163 clinid postflexion larvae clinids 

61100 1154 Paraclinus fasciatus eggs banded blenny 

61101 1155 Paraclinus fasciatus preflexion larvae banded blenny 

61102 1156 Paraclinus fasciatus flexion larvae banded blenny 

61103 1157 Paraclinus fasciatus postflexion larvae banded blenny 

61104 1158 Paraclinus fasciatus juveniles banded blenny 

61105 1159 Paraclinus fasciatus adults banded blenny 

62001 1164 blenniid preflexion larvae blennies 

62210 1165 Chasmodes saburrae eggs Florida blenny 

62211 1166 Chasmodes saburrae preflexion larvae Florida blenny 

62212 1167 Chasmodes saburrae flexion larvae Florida blenny 

62213 1168 

Chasmodes saburrae postflexion 

larvae 

Florida blenny 

62214 1169 Chasmodes saburrae juveniles Florida blenny 

62215 1170 Chasmodes saburrae adults Florida blenny 

62300 1171 Hypsoblennius spp. eggs blennies 

62301 1172 Hypsoblennius spp. preflexion larvae blennies 

62302 1173 Hypsoblennius spp. flexion larvae blennies 

62303 1174 Hypsoblennius spp. postflexion larvae blennies



62304 1175 Hypsoblennius spp. juveniles blennies 

62305 1176 Hypsoblennius spp. adults blennies 

62410 1183 Lupinoblennius nicholsi eggs highfin blenny 

62411 1184 

Lupinoblennius nicholsi preflexion 

larvae 

highfin blenny 

62412 1185 Lupinoblennius nicholsi flexion larvae highfin blenny 

62413 1186 

Lupinoblennius nicholsi postflexion 

larvae 

highfin blenny 

62414 1187 Lupinoblennius nicholsi juveniles highfin blenny 

62415 1188 Lupinoblennius nicholsi adults highfin blenny 

63000 1207 gobiid eggs gobies 

63001 1208 gobiid preflexion larvae gobies 

63002 1209 gobiid flexion larvae gobies 

63003 1210 gobiid postflexion larvae gobies 

63110 1201 Bathygobius soporator eggs frillfin goby 

63111 1202 

Bathygobius soporator preflexion 

larvae 

frillfin goby 

63112 1203 Bathygobius soporator flexion larvae frillfin goby 

63113 1204 

Bathygobius soporator postflexion 

larvae 

frillfin goby 

63114 1205 Bathygobius soporator juveniles frillfin goby 

63115 1206 Bathygobius soporator adults frillfin goby 

63200 1223 Gobionellus spp. eggs gobies 

63201 1224 Gobionellus spp. preflexion larvae gobies 

63202 1225 Gobionellus spp. flexion larvae gobies 

63203 1226 Gobionellus spp. postflexion larvae gobies 

63204 1227 Gobionellus spp. juveniles gobies 

63205 1228 Gobionellus spp. adults gobies 

63210 1211 Gobionellus boleosoma eggs darter goby 

63211 1212 

Gobionellus boleosoma preflexion 

larvae 

darter goby 

63212 1213 Gobionellus boleosoma flexion larvae darter goby 

63213 1214 

Gobionellus boleosoma postflexion 

larvae 

darter goby 

63214 1215 Gobionellus boleosoma juveniles darter goby 

63215 1216 Gobionellus boleosoma adults darter goby 

63220 1217 Gobionellus oceanicus eggs highfin goby 

63221 1218 

Gobionellus oceanicus preflexion 

larvae 

highfin goby 

63222 1219 Gobionellus oceanicus flexion larvae highfin goby 

63223 1220 

Gobionellus oceanicus postflexion 

larvae 

highfin goby 

63224 1221 Gobionellus oceanicus juveniles highfin goby 

63225 1222 Gobionellus oceanicus adults highfin goby



63300 1241 Gobiosoma spp. eggs gobies 

63301 1242 Gobiosoma spp. preflexion larvae gobies 

63302 1243 Gobiosoma spp. flexion larvae gobies 

63303 1244 Gobiosoma spp. postflexion larvae gobies 

63304 1245 Gobiosoma spp. juveniles gobies 

63305 1246 Gobiosoma spp. adults gobies 

63310 1229 Gobiosoma bosc eggs naked goby 

63311 1230 Gobiosoma bosc preflexion larvae naked goby 

63312 1231 Gobiosoma bosc flexion larvae naked goby 

63313 1232 Gobiosoma bosc postflexion larvae naked goby 

63314 1233 Gobiosoma bosc juveniles naked goby 

63315 1234 Gobiosoma bosc adults naked goby 

63320 1235 Gobiosoma robustum eggs code goby 

63321 1236 

Gobiosoma robustum preflexion 

larvae 

code goby 

63322 1237 Gobiosoma robustum flexion larvae code goby 

63323 1238 

Gobiosoma robustum postflexion 

larvae 

code goby 

63324 1239 Gobiosoma robustum juveniles code goby 

63325 1240 Gobiosoma robustum adults code goby 

63400 1253 Microgobius spp. eggs gobies 

63401 1254 Microgobius spp. preflexion larvae gobies 

63402 1255 Microgobius spp. flexion larvae gobies 

63403 1256 Microgobius spp. postflexion larvae gobies 

63404 1257 Microgobius spp. juveniles gobies 

63405 1258 Microgobius spp. adults gobies 

63410 1247 Microgobius gulosus eggs clown goby 

63411 1248 Microgobius gulosus preflexion larvae clown goby 

63412 1249 Microgobius gulosus flexion larvae clown goby 

63413 1250 

Microgobius gulosus postflexion 

larvae 

clown goby 

63414 1251 Microgobius gulosus juveniles clown goby 

63415 1252 Microgobius gulosus adults clown goby 

63420 1259 Microgobius thalassinus eggs clown goby 

63421 1260 

Microgobius thalassinus preflexion 

larvae 

green goby 

63422 1261 Microgobius thalassinus flexion larvae green goby 

63423 1262 

Microgobius thalassinus postflexion 

larvae 

green goby 

63424 1263 Microgobius thalassinus juveniles green goby 

63425 1264 Microgobius thalassinus adults green goby 

63500 1195 Dormitator maculatus eggs fat sleeper 

63501 1196 

Dormitator maculatus preflexion 

larvae 

fat sleeper



63502 1197 Dormitator maculatus flexion larvae fat sleeper 

63503 1198 

Dormitator maculatus postflexion 

larvae 

fat sleeper 

63504 1199 Dormitator maculatus juveniles fat sleeper 

63505 1200 Dormitator maculatus adults fat sleeper 

64000 1271 Microdesmus spp. eggs wormfishes 

64001 1272 Microdesmus spp. preflexion larvae wormfishes 

64002 1273 Microdesmus spp. flexion larvae wormfishes 

64003 1274 Microdesmus spp. postflexion larvae wormfishes 

64004 1275 Microdesmus spp. juveniles wormfishes 

64005 1276 Microdesmus spp. adults wormfishes 

64110 1265 Microdesmus longipinnis eggs pink wormfish 

64111 1266 

Microdesmus longipinnis preflexion 

larvae 

pink wormfish 

64112 1267 

Microdesmus longipinnis flexion 

larvae 

pink wormfish 

64113 1268 

Microdesmus longipinnis postflexion 

larvae 

pink wormfish 

64114 1269 Microdesmus longipinnis juveniles pink wormfish 

64115 1270 Microdesmus longipinnis adults pink wormfish 

69100 888 Prionotus spp. eggs searobins 

69101 889 Prionotus spp. preflexion larvae searobins 

69102 890 Prionotus spp. flexion larvae searobins 

69103 891 Prionotus spp. postflexion larvae searobins 

69104 892 Prionotus spp. juveniles searobins 

69105 893 Prionotus spp. adults searobins 

69110 882 Prionotus scitulus eggs leopard searobin 

69111 883 Prionotus scitulus preflexion larvae leopard searobin 

69112 884 Prionotus scitulus flexion larvae leopard searobin 

69113 885 Prionotus scitulus postflexion larvae leopard searobin 

69114 886 Prionotus scitulus juveniles leopard searobin 

69115 887 Prionotus scitulus adults leopard searobin 

69120 894 Prionotus tribulus eggs bighead searobin 

69121 895 Prionotus tribulus preflexion larvae bighead searobin 

69122 896 Prionotus tribulus flexion larvae bighead searobin 

69123 897 Prionotus tribulus postflexion larvae bighead searobin 

69124 898 Prionotus tribulus juveniles bighead searobin 

69125 899 Prionotus tribulus adults bighead searobin 

69130 881 triglid eggs searobins 

70400 1283 Paralichthys spp. eggs flounders 

70401 1284 Paralichthys spp. preflexion larvae flounders 

70402 1285 Paralichthys spp. flexion larvae flounders 

70403 1286 Paralichthys spp. postflexion larvae flounders 

70404 1287 Paralichthys spp. juveniles flounders



70405 1288 Paralichthys spp. adults flounders 

71110 1289 Achirus lineatus eggs lined sole 

71111 1290 Achirus lineatus preflexion larvae lined sole 

71112 1291 Achirus lineatus flexion larvae lined sole 

71113 1292 Achirus lineatus postflexion larvae lined sole 

71114 1293 Achirus lineatus juveniles lined sole 

71115 1294 Achirus lineatus adults lined sole 

71210 1301 Trinectes maculatus eggs hogchoker 

71211 1302 Trinectes maculatus preflexion larvae hogchoker 

71212 1303 Trinectes maculatus flexion larvae hogchoker 

71213 1304 Trinectes maculatus postflexion larvae hogchoker 

71214 1305 Trinectes maculatus juveniles hogchoker 

71215 1306 Trinectes maculatus adults hogchoker 

72110 1295 Symphurus plagiusa eggs blackcheek tonguefish 

72111 1296 Symphurus plagiusa preflexion larvae blackcheek tonguefish 

72112 1297 Symphurus plagiusa flexion larvae blackcheek tonguefish 

72113 1298 Symphurus plagiusa postflexion larvae blackcheek tonguefish 

72114 1299 Symphurus plagiusa juveniles blackcheek tonguefish 

72115 1300 Symphurus plagiusa adults blackcheek tonguefish 

73220 1313 Monacanthus hispidus eggs planehead filefish 

73221 1314 

Monacanthus hispidus preflexion 

larvae 

planehead filefish 

73222 1315 Monacanthus hispidus flexion larvae planehead filefish 

73223 1316 

Monacanthus hispidus postflexion 

larvae 

planehead filefish 

73224 1317 Monacanthus hispidus juveniles planehead filefish 

73225 1318 Monacanthus hispidus adults planehead filefish 

73230 1319 Monacanthus setifer eggs pygmy filefish 

73231 1320 Monacanthus setifer preflexion larvae pygmy filefish 

73232 1321 Monacanthus setifer flexion larvae pygmy filefish 

73233 1322 Monacanthus setifer postflexion larvae pygmy filefish 

73234 1323 Monacanthus setifer juveniles pygmy filefish 

73235 1324 Monacanthus setifer adults pygmy filefish 

74220 1307 Acanthostrocion quadricornis eggs scrawled cowfish 

74221 1308 

Acanthostrocion quadricornis 

preflexiuon larvae 

scrawled cowfish 

74222 1309 

Acanthostrocion quadricornis flexion 

larvae 


74223 1310 




74224 1311 


juveniles 


74225 1312 Acanthostrocion quadricornis adults scrawled cowfish 

75100 1349 Sphoeroides spp. eggs puffers



75101 1350 Sphoeroides spp. preflexion larvae puffers 

75102 1351 Sphoeroides spp. flexion larvae puffers 

75103 1352 Sphoeroides spp. postflexion larvae puffers 

75104 1353 Sphoeroides spp. juveniles puffers 

75105 1354 Sphoeroides spp. adults puffers 

75110 1331 Sphoeroides nephelus eggs southern puffer 

75111 1332 

Sphoeroides nephelus preflexion 

larvae 

southern puffer 

75112 1333 Sphoeroides nephelus flexion larvae southern puffer 

75113 1334 

Sphoeroides nephelus postflexion 

larvae 

southern puffer 

75114 1335 Sphoeroides nephelus juveniles southern puffer 

75115 1336 Sphoeroides nephelus adults southern puffer 

75120 1343 Sphoeroides spengleri eggs bandtail puffer 

75121 1344 

Sphoeroides spengleri preflexion 

larvae 

bandtail puffer 

75122 1345 Sphoeroides spengleri flexion larvae bandtail puffer 

75123 1346 

Sphoeroides spengleri postflexion 

larvae 

bandtail puffer 

75124 1347 Sphoeroides spengleri juveniles bandtail puffer 

75125 1348 Sphoeroides spengleri adults bandtail puffer 

75130 1337 Sphoeroides parvus eggs least puffer 

75131 1338 Sphoeroides parvus preflexion larvae least puffer 

75132 1339 Sphoeroides parvus flexion larvae least puffer 

75133 1340 Sphoeroides parvus postflexion larvae least puffer 

75134 1341 Sphoeroides parvus juveniles least puffer 

75135 1342 Sphoeroides parvus adults least puffer 

76110 1325 Chilomycterus schoepfi eggs striped burrfish 

76111 1326 

Chilomycterus schoepfi preflexion 

larvae 

striped burrfish 

76112 1327 Chilomycterus schoepfi flexion larvae striped burrfish 

76113 1328 

Chilomycterus schoepfi postflexion 

larvae 

striped burrfish 

76114 1329 Chilomycterus schoepfi juveniles striped burrfish 

76115 1330 Chilomycterus schoepfi adults striped burrfish 

77110 618 Notemigonus crysoleucas eggs golden shiner 

77111 619 

Notemigonus crysoleucas preflexion 

larvae 

golden shiner 

77112 620 

Notemigonus crysoleucas flexion 

larvae 

golden shiner 

77113 621 

Notemigonus crysoleucas postflexion 

larvae 

golden shiner 

77114 622 Notemigonus crysoleucas juveniles golden shiner 

77115 623 Notemigonus crysoleucas adults golden shiner



77200 636 Notropis spp. eggs minnows 

77201 637 Notropis spp. preflexion larvae minnows 

77202 638 Notropis spp. flexion larvae minnows 

77203 639 Notropis spp. postflexion larvae minnows 

77204 640 Notropis spp. juveniles minnows 

77205 641 Notropis spp. adults minnows 

77210 624 Notropis maculatus eggs taillight shiner 

77211 625 Notropis maculatus preflexion larvae taillight shiner 

77212 626 Notropis maculatus flexion larvae taillight shiner 

77213 627 Notropis maculatus postflexion larvae taillight shiner 

77214 628 Notropis maculatus juveniles taillight shiner 

77215 629 Notropis maculatus adults taillight shiner 

77220 630 Notropis petersoni eggs taillight shiner 

77221 631 Notropis petersoni preflexion larvae coastal shiner 

77222 632 Notropis petersoni flexion larvae coastal shiner 

77223 633 Notropis petersoni postflexion larvae coastal shiner 

77224 634 Notropis petersoni juveniles coastal shiner 

77225 635 Notropis petersoni adults coastal shiner 

80100 957 Lepomis spp. eggs sunfishes 

80101 958 Lepomis spp. preflexion larvae sunfishes 

80102 959 Lepomis spp. flexion larvae sunfishes 

80103 960 Lepomis spp. postflexion larvae sunfishes 

80104 961 Lepomis spp. juveniles sunfishes 

80105 962 Lepomis spp. adults sunfishes 

80110 927 Lepomis gulosus eggs warmouth 

80111 928 Lepomis gulosus preflexion larvae warmouth 

80112 929 Lepomis gulosus flexion larvae warmouth 

80113 930 Lepomis gulosus postflexion larvae warmouth 

80114 931 Lepomis gulosus juveniles warmouth 

80115 932 Lepomis gulosus adults warmouth 

80120 933 Lepomis macrochirus eggs bluegill 

80121 934 

Lepomis macrochirus preflexion 

larvae 

bluegill 

80122 935 Lepomis macrochirus flexion larvae bluegill 

80123 936 

Lepomis macrochirus postflexion 

larvae 

bluegill 

80124 937 Lepomis macrochirus juveniles bluegill 

80125 938 Lepomis macrochirus adults bluegill 

80130 945 Lepomis microlophus eggs redear sunfish 

80131 946 

Lepomis microlophus preflexion 

larvae 

redear sunfish 

80132 947 Lepomis microlophus flexion larvae redear sunfish 

80133 948 

Lepomis microlophus postflexion 

larvae 

redear sunfish



80134 949 Lepomis microlophus juveniles redear sunfish 

80135 950 Lepomis microlophus adults redear sunfish 

80140 921 Lepomis auritus eggs redbreast sunfish 

80141 922 Lepomis auritus preflexion larvae redbreast sunfish 

80142 923 Lepomis auritus flexion larvae redbreast sunfish 

80143 924 Lepomis auritus postflexion larvae redbreast sunfish 

80144 925 Lepomis auritus juveniles redbreast sunfish 

80145 926 Lepomis auritus adults redbreast sunfish 

80150 951 Lepomis punctatus eggs spotted sunfish 

80151 952 Lepomis punctatus preflexion larvae spotted sunfish 

80152 953 Lepomis punctatus flexion larvae spotted sunfish 

80153 954 Lepomis punctatus postflexion larvae spotted sunfish 

80154 955 Lepomis punctatus juveniles spotted sunfish 

80155 956 Lepomis punctatus adults spotted sunfish 

80160 939 Lepomis marginatus eggs dollar sunfish 

80161 940 Lepomis marginatus preflexion larvae dollar sunfish 

80162 941 Lepomis marginatus flexion larvae dollar sunfish 

80163 942 

Lepomis marginatus postflexion 

larvae 

dollar sunfish 

80164 943 Lepomis marginatus juveniles dollar sunfish 

80165 944 Lepomis marginatus adults dollar sunfish 

80210 963 Micropterus salmoides eggs largemouth bass 

80211 964 

Micropterus salmoides preflexion 

larvae 

largemouth bass 

80212 965 Micropterus salmoides flexion larvae largemouth bass 

80213 966 

Micropterus salmoides postflexion 

larvae 

largemouth bass 

80214 967 Micropterus salmoides juveniles largemouth bass 

80215 968 Micropterus salmoides adults largemouth bass 

80310 969 Pomoxis nigromaculatus eggs black crappie 

80311 970 

Pomoxis nigromaculatus preflexion 

larvae 

black crappie 

80312 971 

Pomoxis nigromaculatus flexion 

larvae 

black crappie 

80313 972 

Pomoxis nigromaculatus postflexion 

larvae 

black crappie 

80314 973 Pomoxis nigromaculatus juveniles black crappie 

80315 974 Pomoxis nigromaculatus adults black crappie 

80410 1124 Elassoma evergladei eggs Everglades pygmy sunfish 

80411 1125 Elassoma evergladei preflexion larvae Everglades pygmy sunfish 

80412 1126 Elassoma evergladei flexion larvae Everglades pygmy sunfish 

80413 1127 

Elassoma evergladei postflexion 

larvae 

Everglades pygmy sunfish 

80414 1128 Elassoma evergladei juveniles Everglades pygmy sunfish



80415 1129 Elassoma evergladei adults Everglades pygmy sunfish 

80420 1130 Elassoma okefenoke eggs Okefenokee pygmy sunfish 

80421 1131 Elassoma okefenoke preflexion larvae Okefenokee pygmy sunfish 

80422 1132 Elassoma okefenoke flexion larvae Okefenokee pygmy sunfish 

80423 1133 

Elassoma okefenoke postflexion 

larvae 

Okefenokee pygmy sunfish 

80424 1134 Elassoma okefenoke juveniles Okefenokee pygmy sunfish 

80425 1135 Elassoma okefenoke adults Okefenokee pygmy sunfish 

80510 915 Enneacanthus gloriosus eggs bluespotted sunfish 

80511 916 

Enneacanthus gloriosus preflexion 

larvae 

bluespotted sunfish 

80512 917 Enneacanthus gloriosus flexion larvae bluespotted sunfish 

80513 918 

Enneacanthus gloriosus postflexion 

larvae 

bluespotted sunfish 

80514 919 Enneacanthus gloriosus juveniles bluespotted sunfish 

80515 920 Enneacanthus gloriosus adults bluespotted sunfish 

81001 912 centrarchid preflexion larvae sunfishes 

81002 913 centrarchid flexion larvae sunfishes 

81003 914 centrarchid postflexion larvae sunfishes 

82100 1142 Tilapia spp. eggs tilapias 

82101 1147 Tilapia spp. preflexion larvae tilapias 

82102 1143 Tilapia spp. flexion larvae tilapias 

82103 1146 Tilapia spp. postflexion larvae tilapias 

82104 1144 Tilapia spp. juveniles tilapias 

82105 1145 Tilapia spp. adults tilapias 

82114 1140 Tilapia melanotheron juveniles blackchin tilapia 

82115 1141 Tilapia melanotheron adults blackchin tilapia 

82204 1052 Oreochromis mossambicus juveniles Mozambique tilapia 

82205 1053 Oreochromis mossambicus adults Mozambique tilapia 

82304 1138 Oreochromis mossambicus juveniles Mozambique tilapia 

82305 1139 Oreochromis mossambicus adults Mozambique tilapia 

85110 660 Ameiurus natalis eggs yellow bullhead 

85111 661 Ameiurus natalis preflexion larvae yellow bullhead 

85112 662 Ameiurus natalis flexion larvae yellow bullhead 

85113 663 Ameiurus natalis postflexion larvae yellow bullhead 

85114 664 Ameiurus natalis juveniles yellow bullhead 

85115 665 Ameiurus natalis adults yellow bullhead 

85120 654 Ameiurus catus eggs white catfish 

85121 655 Ameiurus catus preflexion larvae white catfish 

85122 656 Ameiurus catus flexion larvae white catfish 

85123 657 Ameiurus catus postflexion larvae white catfish 

85124 658 Ameiurus catus juveniles white catfish 

85125 659 Ameiurus catus adults white catfish 

85130 672 Ictalurus punctatus eggs channel catfish



85131 673 Ictalurus punctatus preflexion larvae channel catfish 

85132 674 Ictalurus punctatus flexion larvae channel catfish 

85133 675 Ictalurus punctatus postflexion larvae channel catfish 

85134 676 Ictalurus punctatus juveniles channel catfish 

85135 677 Ictalurus punctatus adults channel catfish 

85140 666 Ameiurus nebulosus eggs brown bullhead 

85141 667 Ameiurus nebulosus preflexion larvae brown bullhead 

85142 668 Ameiurus nebulosus flexion larvae brown bullhead 

85143 669 

Ameiurus nebulosus postflexion 

larvae 

brown bullhead 

85144 670 Ameiurus nebulosus juveniles brown bullhead 

85145 671 Ameiurus nebulosus adults brown bullhead 

85190 690 Clarias batrachus eggs walking catfish 

85191 691 Clarias batrachus preflexion larvae walking catfish 

85192 692 Clarias batrachus flexion larvae walking catfish 

85193 693 Clarias batrachus postflexion larvae walking catfish 

85194 694 Clarias batrachus juveniles walking catfish 

85195 695 Clarias batrachus adults walking catfish 

85200 678 Noturus gyrinus eggs tadpole madtom 

85201 679 Noturus gyrinus preflexion larvae tadpole madtom 

85202 680 Noturus gyrinus flexion larvae tadpole madtom 

85203 681 Noturus gyrinus postflexion larvae tadpole madtom 

85204 682 Noturus gyrinus juveniles tadpole madtom 

85205 683 Noturus gyrinus adults tadpole madtom 

86000 702 loricariid eggs suckermouth catfishes 

86001 703 loricariid preflexion larvae suckermouth catfishes 

86002 704 loricariid flexion larvae suckermouth catfishes 

86003 705 loricariid postflexion larvae suckermouth catfishes 

86004 706 loricariid juveniles suckermouth catfishes 

86005 707 loricariid adults suckermouth catfishes 

86100 715 Liposarcus spp. eggs suckermouth catfish 

86101 716 Liposarcus spp. preflexion larvae suckermouth catfish 

86102 717 Liposarcus spp. flexion larvae suckermouth catfish 

86103 718 Liposarcus spp. postflexion larvae suckermouth catfish 

86104 719 Liposarcus spp. juveniles suckermouth catfish 

86105 720 Liposarcus spp. adults suckermouth catfish 

86114 708 loricariid sp. b juveniles suckermouth catfish 

86200 709 Hoplosternum littorale eggs brown hoplo catfish 

86201 710 

Hoplosternum littorale preflexion 

larvae 

brown hoplo catfish 

86202 711 Hoplosternum littorale flexion larvae brown hoplo catfish 

86203 712 

Hoplosternum littorale postflexion 

larvae 

brown hoplo catfish 

86204 713 Hoplosternum littorale juveniles brown hoplo catfish



86205 714 Hoplosternum littorale adults brown hoplo catfish 

87000 648 Misgurnus anguillicaudatus eggs oriental weatherfish 

87001 649 

Misgurnus anguillicaudatus preflexion 

larvae 

oriental weatherfish 

87002 650 

Misgurnus anguillicaudatus flexion 

larvae 


87003 651 

Misgurnus anguillicaudatus 



87004 652 Misgurnus anguillicaudatus juveniles oriental weatherfish 

87005 653 Misgurnus anguillicaudatus adults oriental weatherfish 

87100 975 Etheostoma fusiforme eggs swamp darter 

87101 976 

Etheostoma fusiforme preflexion 

larvae 

swamp darter 

87102 977 Etheostoma fusiforme flexion larvae swamp darter 

87103 978 

Etheostoma fusiforme postflexion 

larvae 

swamp darter 

87104 979 Etheostoma fusiforme juveniles swamp darter 

87105 980 Etheostoma fusiforme adults swamp darter 

88110 642 Erimyzon sucetta eggs lake chubsucker 

88111 643 Erimyzon succeta preflexion larvae lake chubsucker 

88112 644 Erimyzon sucetta flexion larvae lake chubsucker 

88113 645 Erimyzon sucetta postflexion larvae lake chubsucker 

88114 646 Erimyzon sucetta juveniles lake chubsucker 

88115 647 Erimyzon sucetta adults lake chubsucker 

100000 1355 tetraodontid eggs puffers 

100001 1356 tetraodontid preflexion larvae puffers 

100002 1358 tetraodontid flexion larvae puffers 

100003 1359 tetraodontid postflexion larvae puffers 

101000 1189 Diplogrammus pauciradiatus eggs spotted dragonet 

101001 1190 

Diplogrammus pauciradiatus 


spotted dragonet 

101002 1191 

Diplogrammus pauciradiatus flexion 

larvae 


101003 1192 

Diplogrammus pauciradiatus 



101004 1193 Diplogrammus pauciradiatus juveniles spotted dragonet 

101005 1194 Diplogrammus pauciradiatus adults spotted dragonet 

110101 1361 unidentified preflexion larvae fish 

110102 1362 unidentified flexion larvae fish 

110103 1363 unidentified postflexion larvae fish 

200000 880 fish eggs, percomorph sciaenid eggs (primarily) 

202000 172 amphipods, gammaridean amphipods 

202100 173 Ampelisca spp. amphipods 

202200 174 Cerapus sp. amphipod



202300 175 Corophium spp. amphipods 

202400 176 Grandidierella bonnieroides amphipod 

202500 177 Gammarus nr. tigrinus amphipod 

202600 178 Cymadusa compta amphipod 

202700 179 Mononuclodes sp. amphipod 

202800 180 Gammarus mucronatus amphipod 

202900 181 Gammarus macromucronatus amphipod 

203000 212 cumaceans cumaceans 

203100 213 Almyracuma sp. cumacean 

203200 214 Cyclaspis varians cumacean 

203300 215 Oxyurostylus smithi cumacean 

204000 184 isopods, unidentified isopods 

204010 185 isopod sp. a isopod 

204023 206 Isopod, sp. b isopod 

204100 186 Munna reynoldsi isopod 

204200 187 Xenanthura brevitelson isopod 

204300 188 Cyathura polita isopod 

204400 189 isopods, unidentified sphaeromatids isopods 

204410 190 Sphaeroma quadridentata isopod 

204420 191 Harrieta faxoni isopod 

204430 192 Cassidinidea ovalis isopod 

204440 193 Sphaeroma walkeri isopod 

204450 194 Sphaeroma terebrans isopod 

204460 207 Isopod, Paracerceis caudata isopod 

204500 195 Edotea triloba isopod 

204510 196 Erichsonella attenuata isopod 

204511 197 Erichsonella filiforme isopod 

204600 198 cymothoid sp. a (Lironeca) juveniles isopod 

204610 199 cymothoid sp. b juveniles isopod 

204620 200 cymothoid sp. c juveniles isopod 

204630 201 Olencira praegustator (in mouth) isopod 

204640 202 cymothoid sp. d juveniles isopod 

204700 203 Anopsilana jonesi isopod 

204800 204 Probopyrus sp. (attached ) isopod 

204803 142 Alteutha sp. copepod 

204900 205 Serolis mcgrayi isopod 

205000 158 unidentified Americamysis juveniles opossum shrimps, mysids 

205110 159 Americamysis almyra opossum shrimp, mysid 

205120 160 Americamysis bahia opossum shrimp, mysid 

205130 161 Americamysis stucki opossum shrimp, mysid 

205200 162 Bowmaniella sp. opossum shrimp, mysid 

205210 163 Bowmaniella dissimilis opossum shrimp, mysid 

205310 164 Brasilomysis castroi opossum shrimp, mysid



205410 165 Metamysidopsis swifti opossum shrimp, mysid 

205510 166 Mysidopsis furca opossum shrimp, mysid 

205520 167 Mysidopsis mortenseni opossum shrimp, mysid 

205610 168 Taphromysis bowmani opossum shrimp, mysid 

205700 169 Spelaeomysis sp. opossum shrimp, mysid 

206000 114 copepod nauplii copepod larvae 

206100 115 unidentified calanoids copepods 

206110 116 Labidocera aestiva copepod 

206120 117 Acartia tonsa copepod 

206121 118 calanoid sp. a copepod 

206130 119 Centropages velificatus copepod 

206140 120 Pseudodiaptomus coronatus copepod 

206150 121 Centropages hamatus copepod 

206160 122 paracalanids copepods 

206170 123 Diaptomus spp. copepods 

206180 124 Eucalanus sp. copepod 

206190 125 Calanopia americana copepod 

206200 131 unidentified freshwater cyclopoids copepods 

206210 132 Oithona spp. copepods 

206220 133 Mesocyclops edax copepod 

206230 134 Orthocyclops modestus copepod 

206240 135 Halicyclops sp. copepod 

206250 136 Macrocyclops albidus copepods 

206260 137 Mesocyclops leuckarti copepod 

206270 138 Cyclops spp. copepods 

206280 139 Saphirella spp. copepods 

206290 140 Eucyclops speratus copepod 

206300 143 unidentified harpacticoids copepods 

206400 147 siphonostomatids parasitic copepods 

206410 144 Sapphirina spp. copepods 

206500 148 Monstrilla sp. copepod 

206600 126 Tortanus setacaudatus copepod 

206610 127 Eurytemora affinis copepod 

206620 128 Temora turbinata copepod 

206630 129 Osphranticum labronectum copepod 

206700 141 Ergasilus spp. parasitic copepods 

206800 130 diaptomids copepods 

206810 145 Oncaea spp. copepods 

206820 146 Corycaeus spp. copepods 

207000 83 cladocerans, unidentified water fleas 

207200 84 Evadne tergestina water flea 

207300 85 Penilia avirostris water flea 

207400 86 cladocerans, daphniid water fleas



207410 87 cladocerans, Daphnia spp. water fleas 

207420 88 Simocephalus vetulus water flea 

207430 89 Scaphroleberis kingi water flea 

207440 90 Ceridodaphnia sp. water flea 

207500 91 Ilyocryptus sp. water flea 

207510 92 Bunops sp. water flea 

207520 93 Grimaldina brazzai water flea 

207600 94 Diaphanosoma brachyurum water flea 

207610 95 Latona setifera water flea 

207620 96 Pseudosida bidentata water flea 

207630 97 Sida crystallina water flea 

207640 98 Latonopsis fasciculata water flea 

207700 99 Bosminopsis deitersi water flea 

207710 100 Bosmina sp. water flea 

207800 101 Euryalona occidentalis water flea 

207810 110 Eurycercus lamellatus water flea 

207900 102 Leydigia sp. water flea 

207910 103 Moinadaphnia macleayii water flea 

207920 104 Eurycercus lamellatus water flea 

207930 105 Dadaya macrops water flea 

207940 106 Camptocercus rectirostris water flea 

207950 107 Anchistropus minor water flea 

207960 108 Alona monacantha water flea 

207970 109 Kurzia longirostris water flea 

208100 67 dipterans, pupae flies, mosquitoes 

208200 68 

dipteran, Chaoborus punctipennis 

larvae 

phantom midge 

208300 69 dipterans, chironomid larvae midges 

208400 70 dipterans, tabanid larvae deer flies 

208500 71 dipterans, ceratopogonid larvae biting midges 

208600 72 dipterans, simuliid larvae black flies 

208700 73 dipterans, tipulid larvae crane flies 

208800 74 dipterans, muscid larvae muscid flies 

208900 75 dipterans, stratiomyid larvae soldier flies 

208910 76 dipterans, sciomyzid larvae marsh flies 

208920 77 dipterans, dolichopodid larvae dolichopodid flies 

208921 79 dipterans, ephydrid larvae shore flies 

208930 78 dipterans, syrphid larvae hoverflies 

209000 482 decapod zoeae crab larvae 

209100 477 decapod zoeae, pinnotherid crab larvae 

209101 483 decapod zoeae, spineless crab larvae 

209200 350 decapod zoeae, porcellanid crab larvae 

209300 415 decapod zoeae, xanthid crab larvae 

210000 149 ostracods, unidentified ostracods, seed shrimps



210100 150 Parasterope pollex ostracod, seed shrimp 

210200 154 ostracods, podocopid ostracods, seed shrimps 

210300 151 Sarsiella zostericola ostracod, seed shrimp 

210400 152 Euconchoecia chierchiae ostracod, seed shrimp 

210500 153 myodocopod sp. a ostracod, seed shrimp 

211000 20 Mnemiopsis mccradyi comb jelly, ctenophore 

211100 21 Beroe ovata sea walnut, ctenophore 

213000 484 decapod megalopae post-zoea crab larvae 

213100 417 

decapod megalops larvae type 1 

(xanthid) 

post-zoea crab larvae 

213200 351 decapod megalops larvae, porcellanid post-zoea crab larvae 

213300 400 decapod megalops larvae, portunid post-zoea crab larvae 

213301 401 Cancer spp. zoea larvae crabs 

213303 402 Cancer spp. megalops larvae crabs 

213304 403 Cancer spp. juveniles crabs 

213305 404 Cancer spp. adults crabs 

213400 416 decapod megalops larvae, xanthid post-zoea crab larvae 

214000 339 decapod mysis shrimp larvae 

214200 323 decapod mysis, callianassid shrimp larvae 

214300 248 decapod mysis, palaemonid shrimp larvae 

214500 342 decapod mysis, pagurid hermit crab larvae 

214800 310 decapod mysis, Ogyrides shrimp larvae 

215000 216 Lucifer faxoni juveniles and adults shrimp 

215002 217 Lucifer faxoni mysis shrimp 

216000 496 chaetognaths, sagittid arrow worms 

216100 497 Chaetognaths, Ferrosagitta hispida arrow worm 

216200 498 Chaetognaths, Flaccisagitta enflata arrow worm 

216300 499 Chaetognaths, Sagitta tenuis arrow worm 

217000 25 polychaetes sand worms, tube worms 

218000 16 Clytia sp. hydromedusa 

218100 18 Liriope tetraphylla hydromedusa 

218200 15 Craspedacusta sowberii hydromedusa 

218300 6 medusa sp. a hydromedusa 

218400 7 medusa sp. b hydromedusa 

218500 8 medusa sp. c hydromedusa 

218600 9 medusa sp. d hydromedusa 

218700 10 medusa sp. e hydromedusa 

218800 11 medusa, Obelia sp. hydromedusa 

218900 13 medusa, Bougainvillia sp. hydromedusa 

219000 488 pelecypods clams, mussels, oysters 

220100 489 gastropods, prosobranch snails 

220200 490 gastropods, opisthobranch sea slugs 

221000 26 oligochaetes freshwater worms 

222100 35 odonates, zygopteran larvae damselflies



222200 36 odonates, anisopteran larvae dragonflies 

223002 249 Palaemonetes spp. mysis larvae grass shrimps 

223003 250 Palaemonetes spp. postlarvae grass shrimps 

223104 251 Palaemonetes pugio juveniles daggerblade grass shrimp 

223105 252 Palaemonetes pugio adults daggerblade grass shrimp 

223114 253 Palaemonetes vulgaris juveniles grass shrimp 

223115 254 Palaemonetes vulgaris adults grass shrimp 

223124 255 Palaemonetes paludosus juveniles grass shrimp 

223125 256 Palaemonetes paludosus adults grass shrimp 

223134 257 Palaemonetes intermedius juveniles grass shrimp 

223135 258 Palaemonetes intermedius adults grass shrimp 

224002 218 penaeid protozoeae penaeid shrimps 

224003 220 penaeid postlarvae penaeid shrimps 

224003.5 221 penaeid metamorphs penaeid shrimps 

224104 222 Farfantepenaeus duorarum juveniles pink shrimp 

224105 223 Farfantepenaeus duorarum adults pink shrimp 

225000 113 branchiurans, Argulus spp. fish lice 

226000 182 amphipods, caprellid skeleton shrimps 

227100 486 Lolliguncula brevis juveniles bay squid 

227105 487 Lolliguncula brevis adults bay squid 

228000 111 cirriped nauplius stage barnacles 

228003 112 cirriped cypris stage barnacles 

229000 495 appendicularian, Oikopleura dioica larvacean 

230101 430 grapsid zoea larvae crabs 

230103 431 grapsid megalops larvae crabs 

230104 432 grapsid juveniles crabs 

230113 433 Aratus pisonii megalops larvae mangrove tree crab 

230114 434 Aratus pisonii juveniles mangrove tree crab 

230115 435 Aratus pisonii adults mangrove tree crab 

230201 405 xanthid zoea larvae mud crabs 

230203 406 xanthid megalops larvae mud crabs 

230204 407 xanthid juveniles mud crabs 

230205 408 xanthid adults mud crabs 

230211 436 Rhithropanopeus harrisii zoea larvae Harris mud crab 

230213 437 

Rhithropanopeus harrisii megalops 

larvae 

Harris mud crab 

230214 438 Rhithropanopeus harrisii juveniles Harris mud crab 

230215 439 Rhithropanopeus harrisii adults Harris mud crab 

230221 409 Eurypanopeus depressus zoea larvae flatback mud crab 

230223 410 

Eurypanopeus depressus megalops 

larvae 

flatback mud crab 

230224 411 Eurypanopeus depressus juveniles flatback mud crab 

230225 412 Eurypanopeus depressus adults flatback mud crab 

230231 418 Panopeus herbstii complex zoea larvae mud crab



230233 419 

Panopeus herbstii complex megalops 

larvae 

mud crab 

230234 420 Panopeus herbstii complex juveniles mud crab 

230235 421 Panopeus herbstii complex adults mud crab 

230241 422 Menippe mercenaria zoea larvae Florida stone crab 

230243 423 Menippe mercenaria megalops larvae Florida stone crab 

230244 424 Menippe mercenaria juveniles Florida stone crab 

230245 425 Menippe mercenaria adults Florida stone crab 

230251 413 xanthid type a zoea larvae mud crab 

230253 414 xanthid type a megalops larvae mud crab 

230254 428 Neopanope spp. juveniles crabs 

230255 429 Neopanope spp. adults crabs 

230301 388 Callinectes sapidus zoea larvae blue crab 

230303 389 Callinectes sapidus megalops larvae blue crab 

230304 390 Callinectes sapidus juveniles blue crab 

230305 391 Callinectes sapidus adults blue crab 

230311 392 Portunus spp. zoea larvae swimming crabs 

230313 393 Portunus spp. megalops larvae swimming crabs 

230314 394 Portunus sp. juveniles swimming crab 

230315 395 Portunus sp. adults swimming crab 

230321 396 portunid zoea larvae swimming crab 

230323 397 portunid megalops larvae swimming crabs 

230324 398 portunid juveniles swimming crabs 

230325 399 portunid adults swimming crabs 

230401 452 pinnotherid zoea larvae pea crabs 

230403 453 pinnotherid megalops larvae pea crabs 

230404 454 pinnotherid juveniles pea crabs 

230405 455 pinnotherid adults pea crabs 

230411 456 Pinnotheres maculatus zoea larvae squatter pea crab 

230413 457 

Pinnotheres maculatus megalops 

larvae 

squatter pea crab 

230414 458 Pinnotheres maculatus juveniles squatter pea crab 

230415 459 Pinnotheres maculatus adults squatter pea crab 

230421 460 Pinnixa sayana zoea larvae pea crab 

230423 461 Pinnixa sayana megalops larvae pea crab 

230424 462 Pinnixa sayana juveniles pea crab 

230425 463 Pinnixa sayana adults pea crab 

230431 464 Pinnotheres hemphilli zoea larvae pea crab 

230433 465 Pinnotheres hemphilli megalops larvae pea crab 

230434 466 Pinnotheres hemphilli juveniles pea crab 

230435 467 Pinnotheres hemphilli adults pea crab 

230441 468 Pinnixa sp. a zoea larvae pea crab 

230443 469 Pinnixa sp. a megalops larvae pea crab 

230444 470 Pinnixa sp. a juveniles pea crab



230445 471 Pinnixa sp. a adults pea crab 

230451 472 Pinnixa sp. b zoea larvae pea crab 

230453 473 Pinnixa sp. b megalops larvae pea crab 

230454 474 Pinnixa sp. b juveniles pea crab 

230455 475 Pinnixa sp. b adults pea crab 

230461 476 Pinnotheres spp. zoea larvae pea crab 

230503 378 majid megalops larvae spider crabs 

230504 379 majid juveniles spider crabs 

230511 380 Libinia dubia zoea larvae longnose spider crab 

230513 381 Libinia dubia megalops larvae longnose spider crab 

230514 382 Libinia dubia juveniles longnose spider crab 

230515 383 Libinia dubia adults longnose spider crab 

230701 343 paguroid zoea larvae hermit crabs 

230703 344 paguroid megalops larvae hermit crabs 

230704 345 paguroid juveniles hermit crabs 

230711 346 Pagurus longicarpus zoea larvae long-clawed hermit crab 

230713 347 Pagurus longicarpus megalops larvae long-clawed hermit crab 

230714 348 Pagurus longicarpus juveniles long-clawed hermit crab 

230715 349 Pagurus longicarpus adults long-clawed hermit crab 

230801 352 

porcellanid (not P. armatus) zoea 

larvae 

porcelain crabs 

230803 353 

porcellanid (not P. armatus) megalops 

larvae 

porcelain crabs 

230804 354 porcellanid (not P. armatus) juveniles porcelain crabs 

230805 355 porcellanid (not P. armatus) adults porcelain crabs 

230811 356 Petrolisthes armatus zoea larvae porcelain crab 

230813 357 Petrolisthes armatus megalops larvae porcelain crab 

230814 358 Petrolisthes armatus juveniles porcelain crab 

230815 359 Petrolisthes armatus adults porcelain crab 

230821 360 Euceramus praelongus zoea larvae olivepit porcelain crab 

230823 361 

Euceramus praelongus megalops 

larvae 

olivepit porcelain crab 

230824 362 Euceramus praelongus juveniles olivepit porcelain crab 

230825 363 Euceramus praelongus adults olivepit porcelain crab 

230834 364 Petrolisthes galathinus juveniles banded porcelain crab 

230835 365 Petrolisthes galathinus adults banded porcelain crab 

230901 479 Uca spp. zoea larvae fiddler crabs 

230903 480 Uca spp. megalops larvae fiddler crabs 

230904 481 Uca spp. juveniles fiddler crabs 

230911 440 Sesarma reticulatum zoea larvae marsh crab 

230913 441 Sesarma reticulatum megalops larvae marsh crab 

230914 442 Sesarma reticulatum juveniles marsh crab 

230915 443 Sesarma reticulatum adults marsh crab 

230921 444 Sesarma cinereum zoea larvae marsh crab



230923 445 Sesarma cinereum megalops larvae marsh crab 

230924 446 Sesarma cinereum juveniles marsh crab 

230925 447 Sesarma cinereum adults marsh crab 

230931 448 Sesarma spp. zoea larvae marsh crab 

230933 449 Sesarma spp. megalops larvae marsh crab 

230934 450 Sesarma spp. juveniles marsh crab 

230935 451 Sesarma spp. adults marsh crab 

231000 156 Squilla empusa larvae mantis shrimp 

231004 157 Squilla empusa juveniles mantis shrimp 

232000 1364 anuran larvae tadpoles 

233000 208 tanaids, unidentified tanaids 

233100 209 Hargeria rapax tanaid 

233200 210 Sinelobus stanfordi tanaid 

233300 211 Apseudes sp. tanaid 

234000 63 coleopterans, unident. larvae beetles 

234100 47 coleopterans, noterid larvae burrowing water beetles 

234105 48 coleopterans, noterid adults burrowing water beetles 

234200 49 coleopterans, elmid larvae riffle beetles 

234205 50 coleopterans, elmid adults riffle beetles 

234210 51 coleopterans, lutrochid larvae beetles 

234305 52 coleopterans, curculionid adults beetles 

234400 53 coleopterans, dytiscid larvae predaceous diving beetles 

234405 54 coleopterans, dytiscid adults predaceous diving beetles 

234505 56 coleopterans, haliplid adults crawling water beetles 

234520 55 coleopterans, haliplid larvae crawling water beetles 

234600 57 coleopterans, gyrinid larvae whirligig beetles 

234605 58 coleopterans, gyrinid adults whirligig beetles 

234700 59 coleopterans, scirtid larvae marsh beetles 

234705 60 coleopterans, scirtid adults marsh beetles 

234800 61 coleopterans, chrysomelid larvae beetles 

234900 62 coleopterans, dryopid larvae long-toed water beetles 

235000 31 acari water mites 

236003 29 Limulus polyphemus larvae horsehoe crab 

237002 331 Upogebia spp. mysis larvae mud shrimps 

237003 332 Upogebia spp. postlarvae mud shrimps 

237004 333 Upogebia spp. juveniles mud shrimps 

237005 334 Upogebia spp. adults mud shrimps 

238000 1 foraminiferans foraminiferans 

239000 30 pycnogonids sea spiders 

240000 491 

brachiopod, Glottidia pyramidata 

larvae 

lamp shell 

242000 312 

Ogyrides alphaerostris juveniles and 

adults 

estuarine longeye shrimp 

242002 311 Ogyrides alphaerostris mysis larvae estuarine longeye shrimp



243002 279 alphaeid mysis larvae snapping shrimps 

243003 280 alphaeid postlarvae snapping shrimps 

243004 281 alphaeid juveniles snapping shrimps 

243112 282 Leptalpheus forceps mysis larvae snapping shrimp 

243113 283 Leptalpheus forceps postlarvae snapping shrimp 

243114 284 Leptalpheus forceps juveniles snapping shrimp 

243115 285 Leptalpheus forceps adults snapping shrimp 

243202 286 Alpheus viridari mysis larvae snapping shrimp 

243203 287 Alpheus viridari postlarvae snapping shrimp 

243204 288 Alpheus viridari juveniles snapping shrimp 

243205 289 Alpheus viridari adults snapping shrimp 

243212 290 Alpheus estuariensis mysis snapping shrimp 

243213 291 Alpheus estuariensis postlarvae snapping shrimp 

243214 292 Alpheus estuariensis juveniles snapping shrimp 

243215 293 Alpheus estuariensis adults snapping shrimp 

244000 22 turbellarians flatworms 

245000 170 mysid free embryos opossum shrimps, mysids 

246000 24 nematodes roundworms, threadworms 

247000 500 Branchiostoma floridae lancelet 

249105 38 hemipterans, corixid adults water boatmen 

249205 37 hemipterans, corixid juveniles water boatmen 

249304 39 hemipterans, naucorid juveniles creeping water bugs 

249305 40 hemipterans, naucorid adults creeoing water bugs 

249405 41 hemipterans, pleid adults pygmy backswimmers 

249505 42 hemipterans, gerrid adults water striders 

249605 43 hemipterans, belostomatid adults giant water bugs 

249715 44 hemipterans, veliid adults short-legged water striders 

249800 45 hemipterans, nepid adults water scorpions 

249900 46 hemipterans, notonectid adults backswimmers 

250002 294 Hippolyte zostericola mysis larvae zostera shrimp 

250003 295 Hippolyte zostericola postlarvae zostera shrimp 

250004 296 Hippolyte zostericola juveniles zostera shrimp 

250005 297 Hippolyte zostericola adults zostera shrimp 

250102 298 Thor sp. mysis larvae shrimp 

250103 299 Thor sp. postlarvae shrimp 

250104 300 Thor sp. juveniles shrimp 

250105 301 Thor sp. adults shrimp 

252000 81 trichopteran larvae caddisflies 

253000 33 ephemeropteran larvae mayflies 

253100 34 ephemeropterans, potamanthid larvae mayflies 

254002 302 Tozeuma carolinense mysis larvae arrow shrimp 

254003 303 Tozeuma carolinense postlarvae arrow shrimp 

254004 304 Tozeuma carolinense juveniles arrow shrimp



254005 305 Tozeuma carolinense adults arrow shrimp 

255004 224 Sicyonia laevigata juveniles rock shrimp 

255005 225 Sicyonia laevigata adults rock shrimp 

255102 226 sicyoniid mysis larvae rock shrimps 

255103 227 sicyoniid postlarvae rock shrimps 

255104 228 sicyoniid juveniles rock shrimps 

256000 64 neuropterans, Climacia spp. larvae spongillaflies 

257000 82 lepidopterans, pyralid larvae aquatic caterpillars 

258000 493 ophiuroidean juveniles brittlestars 

258100 494 ophiopluteus larvae brittlestars 

259002 259 Periclimenes spp. mysis larvae shrimps 

259003 260 Periclimenes spp. postlarvae shrimps 

259004 261 Periclimenes spp. juveniles shrimps 

259005 262 Periclimenes spp. adults shrimps 

259102 263 Periclimenes americanus mysis larvae American grass shrimp 

259103 264 Periclimenes americanus postlarvae American grass shrimp 

259104 265 Periclimenes americanus juveniles American grass shrimp 

259105 266 Periclimenes americanus adults American grass shrimp 

259112 267 

Periclimenes longicaudatus mysis 

larvae 

longtail grass shrimp 

259113 268 Periclimenes longicaudatus postlarvae longtail grass shrimp 

259114 269 Periclimenes longicaudatus juveniles longtail grass shrimp 

259115 270 Periclimenes longicaudatus adults longtail grass shrimp 

260000 3 Chrysaora quinquecirrha sea nettle 

260100 5 Scyphozoan, Rhopilema verrilli jellyfish 

261000 23 nemerteans ribbon worms 

262002 335 Upogebia affinis mysis larvae coastal mud shrimp 

262003 336 Upogebia affinis postlarvae coastal mud shrimp 

262004 337 Upogebia affinis juveniles coastal mud shrimp 

262005 338 Upogebia affinis adults coastal mud shrimp 

263003 340 shrimps, unidentified postlarvae shrimps 

263004 341 shrimps, unidentified juveniles shrimps 

265112 229 Acetes americanus mysis larvae shrimp 

265113 230 Acetes americanus postlarvae shrimp 

265114 231 Acetes americanus juveniles shrimp 

265115 232 Acetes americanus adults shrimp 

266003 233 Rimapenaeus spp. postlarvae shrimps 

266004 234 Rimapenaeus spp. juveniles shrimps 

266005 235 Rimapenaeus spp. adults shrimps 

267003 320 astacidean postlarvae crayfish 

267004 321 astacidean juveniles crayfish 

267005 322 astacidean adults crayfish 

269000 32 collembolas, podurid springtails 

272000 4 Aurelia aurita moon jellyfish



273002 244 Leptochela serratorbita mysis larvae combclaw shrimp 

273003 245 Leptochela serratorbita postlarvae combclaw shrimp 

273004 246 Leptochela serratorbita juveniles combclaw shrimp 

273005 247 Leptochela serratorbita adults combclaw shrimp 

275002 313 Ambidexter symmetricus mysis larvae shrimp 

275003 314 Ambidexter symmetricus postlarvae shrimp 

275004 315 Ambidexter symmetricus juveniles shrimp 

275005 316 Ambidexter symmetricus adults shrimp 

276000 155 leptostracan, Nebalia sp. nebaliid 

277000 27 hirudinoideans leeches 

278002 317 processid mysis larvae night shrimps 

278003 318 processid postlarvae night shrimps 

278004 319 processid juveniles night shrimps 

279002 324 callianassid mysis larvae ghost shrimps 

279003 325 callianassid postlarvae ghost shrimps 

279004 326 callianassid juveniles ghost shrimps 

279102 327 Callianassa spp. mysis larvae ghost shrimps 

279103 328 Callianassa spp. postlarvae ghost shrimps 

279104 329 Callianassa spp. juveniles ghost shrimps 

279105 330 Callianassa spp. adults ghost shrimps 

280002 306 Latreutes parvulus mysis larvae sargassum shrimp 

280003 307 Latreutes parvulus postlarvae sargassum shrimp 

280004 308 Latreutes parvulus juveniles sargassum shrimp 

280005 309 Latreutes parvulus adults sargassum shrimp 

281000 183 Lestrigonus bengalensis hyperiid amphipod 

282000 501 thaliaceans salps 

282001 502 ascidiacean larvae tunicate larvae 

283000 2 scyphozoan ephyrae jellyfish larvae 

284002 236 

Trachypenaeopsis mobilispinus mysis 

larvae 

shrimps 

284003 237 

Trachypenaeopsis mobilispinus 

postlarvae 

shrimps 

284004 238 

Trachypenaeopsis mobilispinus 

juveniles 

shrimps 

284005 239 Trachypenaeopsis mobilispinus adults shrimps 

285000 65 megalopterans, corydalid larvae dobsonflies 

285100 66 megalopterans, sialid larvae alderflies 

287000 503 hemichordate larvae acorn worms 

293000 28 sipunculids peanut worms 

294000 492 chitons chitons 

295000 19 siphonophores siphonophores 

296002 271 Palaemon floridanus mysis larvae Florida grass shrimp 

296003 272 Palaemon floridanus postlarvae Florida grass shrimp 

296004 273 Palaemon floridanus juveniles Florida grass shrimp



296005 274 Palaemon floridanus adults Florida grass shrimp 

297102 275 Leander paulensis mysis larvae grass shrimp 

297103 276 Leander paulensis postlarvae grass shrimp 

297104 277 Leander paulensis juveniles grass shrimp 

297105 278 Leander paulensis adults grass shrimp 

298100 14 

medusa (Hydrocodon forbesi or 

Corymorpha sp.) 

hydromedusa 

298101 374 Crab, Emerita sp. zoea larvae mole crab 

298103 375 Crab, Emerita sp. megalops larvae mole crab 

298104 376 Crab, Emerita sp. juveniles mole crab 

298105 377 Crab, Emerita sp. adults mole crab 

298201 366 Albunia sp. zoea larvae crab 

298203 367 Albunia sp. megalops larvae crab 

298204 368 Albunia sp. juveniles crab 

298205 369 Albunia sp. adults crab 

299000 17 medusa, Eutima sp. hydromedusa 

299001 384 Crab, Persephona sp. zoea larvae crab 

299002 240 Lysmata sp. mysis larvae shrimp 

299003 241 Lysmata sp. postlarvae shrimp 

299004 242 Lysmata sp. juveniles shrimp 

299005 243 Lysmata sp. adults shrimp 

301001 370 Crab, Lepidopa sp. zoea larvae crab 

301003 371 Crab, Lepidopa sp. megalops larvae crab 

301004 372 Crab, Lepidopa sp. juveniles crab 

301005 373 Crab, Lepidopa sp. adults crab

Page 1 

Section 9.0 

April 2008 




McKay Bay and the Alafia Banks are recognized as important areas for a number of 

water-dependent resident and migrating bird species. To address concerns regarding 

potential impacts on bird populations resulting from decreased freshwater flows, a bird 

survey monitoring element was included as part of the initial HBMP design. Avifaunal 

data collection and analysis efforts were initiated after PBS&J obtained technical input 

and guidance from The National Audubon Society and independent ornithologists/field 

biologists. 

As a linkage between bird populations and withdrawals was not determined with the 

initial survey efforts in WY 2000-2003, a new refined HBMP special study was 

developed beginning in WY 2004-2005. The redesigned study discontinued surveys at 

all locations except near the mouth of the Alafia River (i.e., Alafia Banks) where a more 

detailed HBMP special study was initiated to evaluate potential changes in feeding 

patterns of bird species that have some potential to be affected by deceased freshwater 

inflows through Alafia River withdrawals for public water supply. 

9.2 Monitoring Overview 

The Alafia Banks monitoring site (Figure 9.1) is located at the mouth of the Alafia River. 

Monitoring is performed within the mud flats adjacent and proximal to the islands. The 

islands in this area are bird sanctuaries managed by the National Audubon Society. For 

the new special study, feeding surveys are performed for two species, the willet 

(Catoptrophorus semipalmatus) and white ibis (Eudocimus albus). These key indicator 

bird species were identified based on feeding characteristics and historical data through 

consultation with Audubon Society ornithologists. 

This special study includes data collection from the same general area as the previous 

bird element surveys (in particular, the east end of the Alafia Banks and the western 

shoreline immediately east of the Alafia Banks), but also reflect field conditions and the 

availability and location of white ibis and willet as they forage. The surveys continue to 

include the low and high tide surveys on the Alafia Banks as described in Section 9.4. In 

addition, benthic samples are collected to attempt to determine prey availability, in and 

around areas in which feeding ibis and willets are observed. Benthic sampling activities 

reflect weather and tide conditions, but are intended to occur in conjunction with bird 

surveys. Benthic samples are collected and analyzed in accordance with the methods and 

procedures described in Section 6.0 of this QA/QC Plan.

Page 2 

Section 9.0 

April 2008 


9.3 Field Observations 

Surveys are performed to determine the number of bird species present and numbers of 

individuals by species. Surveys are conducted at high and low tides, during one day 

every other month (six times per year). Surveys to determine mean feeding rates occur 

six times per year on low tides only. 

The objectives of the avifauna survey element of the HBMP are as follows: 

• Estimate diversity and richness of selected water-dependent bird species in the 

vicinity of the Alafia River Reporting Unit (i.e., Alafia Banks). 

• Determine if species diversity and richness changes significantly over time. 

• Determine if significant changes to diversity and richness can be related to changes in 

benthos and fish species diversity and richness through analysis of appropriate taxa 

level(s) or guilds. 

• Identify foraging rates of the willet and white ibis, and determine if changes occur 

over time. 

• If changes to foraging rates occur, determine if feeding rate changes can be related to 

prey-base changes. 

• Determine prey species diversity within foraging areas. 

Avifauna surveys will be conducted by a minimum of two PBS&J staff biologists, 

whenever possible, including the Principle Investigator (PI) with a strong background in 

bird identification and surveying techniques (Figure 9.2). Field staff will be trained in all 

collection procedures and will have copies of all the pertinent chapters of the HBMP 

Project Specific QA/QC Document to consult. In addition to regular quarterly QA/QC 

reviews of field procedures, training workshops will be held from time to time to review 

all data collection protocols. This workshop may also be held in conjunction with an 

annual review of the HBMP field safety program. 


Initial field mobilization and preparation should be completed the day before the survey. 

A checklist (Table 9.1) of all necessary supplies and equipment should be used to assure 

field personnel are properly prepared. 

9.3.2 Survey Preparation Tasks 

1. Check and clean the binoculars and spotting scope to ensure that they are in proper 

working order.

Page 3 

Section 9.0 

April 2008 


2. Review an updated Florida Statewide Rare Bird Alert or other pertinent sources to be 

aware of unusual or rare birds that may be encountered during surveys. This 

information can be accessed at http://listserv.admin.usf.edu/archives/brdbrain.html. 

3. Identify and schedule at least three field days for both low and high tide surveys. 

Predictive tides can be obtained at web sites such as: 

http://tidesonline.nos.noaa.gov or 

http://co-ops.nos.noaa.gov/tides05/2ec3e.html#102 

The species and abundance survey events should be completed in two morning 

periods (primary days) for the Alafia River study area, every other month. The 

feeding survey is to be conducted every month, twice per month or as conditions 

permit. 

Any scheduled additional days will provide flexibility in the survey schedule in the 

event inclement weather prevents access to survey areas. All other PBS&J field 

personnel will be notified of scheduled field days and changes to primary survey 

days. 

4. Anticipate unpredicted changes in tide levels by evaluating weather forecasts for 

frontal activity and predominant wind conditions. This information should be 

evaluated no more than two days before scheduled field days so that appropriate 

adjustments to the survey effort can be made. 

5. Fuel and clean the boat and pack all necessary safety gear. Boat batteries should be 

checked and placed on slow charge. 

6. Prepare benthic samplers days prior to a suitable survey event to assure they are in 

working order to collect samples. 

9.3.3 Survey Time and Tide Criteria 

1. High Tide Survey (Species count survey only): peak local high tide levels must meet 

or exceed 2.0 feet or are identified as high tide by tide prediction charts referenced in 

section 9.3.2. Survey start time should be as early in the morning as possible, 

preferably at dawn and should coincide closely with the peak of the tide cycle. 

Surveys should not occur past 1130 hours Eastern Standard Time. 

2. Low Tide Survey (both survey types): peak local low tide levels must be 1.0 foot or 

less, preferably to negative tide levels. Survey start time should be as early in the 

morning as possible, preferably at dawn, and should coincide closely with the peak of 

the tide cycle in the Alafia River study area. Morning surveys should not occur past 

1130 hours Eastern Standard Time. Afternoon surveys should be concluded before

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sunset conditions to avoid low light conditions that would inhibit accurate bird 

identification. 

3. Feeding surveys, in general, will coincide with low tide events. These tides will be 

selected based on anticipated tide levels on site and are not based on a particular 

elevation as a range of elevations is anticipated for this type of survey. 

4. Special concessions should be made in the unusual event that not all of the abovedescribed 

criteria can be met for a survey month. In these instances, the PI should 

select a date that meets as many of these criteria as possible. Additionally, severe 

weather conditions on selected survey days (that meet time and tide criteria) may 

cause the rescheduling of survey date(s). If this occurs, the dates available for 

rescheduling may not meet the time and tide criteria. Again, the PI should use the 

same guidance described above. 

9.3.4 Miscellaneous Materials 

Data forms, list of potential survey sites, waterproof pens, pencils and markers should be 

placed in waterproof containers. 

A complete set of large and small-scale maps should be organized and appropriately 

packed for each reporting unit until data collector becomes familiar with site conditions. 

9.4 Avifauna Survey Data Collection 

9.4.1 Survey Day Field Mobilization 

1. Check timepieces for accuracy and consistency. 

2. Verify that all equipment on the checklist has been placed in the boat and/or vehicle 

for transport to survey sites 

9.4.2 Biotic Data Protocol 

Data collection will include observing and recording all bird species present, if possible, 

and the number of individuals per species. Higher taxon levels should be recorded if 

species cannot be determined (e.g., genus or family – see Table 9.2). 

For feeding surveys, foraging individuals of the willet and/or white ibis shall be 

identified and observed. During observations, data related to foraging period for 

individuals and number of prey captures per individual shall be recorded.

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9.4.3 Biotic Data Recording Logistics 

Surveys will be conducted by boat. The boat must be anchored and secured at the 

sampling station prior to surveying. Occasionally, wave conditions may prevent use of 

either the spotting scope or binoculars. In this instance surveying can be performed by 

standing in the water and using the spotting scope. Hip boots, waders, or a wet suit 

should be used during periods of cool water temperatures. 

9.4.4 Field Data Recording Procedures 

Data forms will be used to record all observations during field surveys. Table 9.2 

presents a sample data recording form for this survey and also lists all species that may 

occur within the three recording units during a calendar year. A sample data form for 

feeding survey is also provided as Table 9.3. Species were selected based upon 

previously known occurrences. Vagrant and rarely observed species were not included in 

this list, but can be recorded under “additional species.” These species are not 

considered valuable indicators of environmental conditions, but will be recorded for other 

uses (e.g., local birding records, Florida Rare Bird Alert). 

9.4.4.1 Standard Species Surveys (Low and High Tides) 

1. Upon arrival at each survey station, record the start time, tidal stage, wind speed, 

wind direction and current weather conditions. 

2. A minimum of 15 minutes of observations and recording for both the standard and 

duplicate surveys is required. Efforts should target recording all taxa and numbers 

within a reasonable period. 

3. Begin recording one species at a time. First, identify a species (or closest taxon 

level), then survey and count the number of individuals observed within the 

designated survey boundary. Repeat this process until all species have been recorded 

and counted. 

4. Limits of survey areas are identified on color infrared aerial photographs. 

Duplicate sampling must also occur at every survey station in order to estimate field 

sampling precision and, possibly, to determine an average number of species and total 

individuals for each station. Surveying and recording for the duplicate sampling should 

begin approximately five minutes after the completion of the initial recording at that 

station. To perform duplicate sampling follow the procedures described in Subsection 3 

above. 

Special Note: Observers should be conscious of minimizing disturbance of birds. 

Minimize movement and noise to the greatest extent possible. Such actions will reduce

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the chance of accidentally “flushing” birds from the sampling area, thereby adversely 

affecting data collection. 

9.4.4.2 Determination of Mean Feeding Rates 

The methodology used to estimate mean feeding rates is based upon that described in 

Moreira (1993) and Goss-Custard (1967). The target species, willet (Catoptrophourus 

semipalmatus) and white ibis (Eudocimus albus) were selected based upon the following 

criteria: 

1. These species are year-round residents. 

2. These species are known to feed on benthic organisms in sand and mud flats near 

the Alafia Banks. 

3. These species have been consistently counted at the Alafia Banks, throughout the 

year, for each of the prior four years of this study. 

4. No other bird species meet the above stated criteria. 

Mean feeding rates will be determined by counting the amount of food-captures a bird 

performs in a given area during short measuring periods. The study areas are the sand 

and mud flats near the Alafia Banks. The surveys will focus primarily on foraging areas 

at the eastern tip of the Banks and nearby shoreline areas. 

Benthic samples shall be evaluated to determine species available as prey. During bird 

feeding observations, attempts should be made to determine what prey species are being 

captured by the birds once that data is available. Prey species and availability may 

change throughout the year, so efforts to identify prey base changes should be conducted. 

9.4.5 Abiotic Data Protocol 

Efforts will be made to record current time, date, tide stage and weather conditions for 

each station. Any unusual conditions will also be noted (i.e. large numbers of dead fish 

or other animals, including species and approximate size range) in the field notebook. 

1. Tide Stage Nomenclature 

• Descriptions of tide stages have been standardized for the HBMP. The following list 

presents these descriptors as they are to be recorded on the data forms. 

• high slack 

• high outgoing 

• mid outgoing 

• low outgoing 

• low slack 

• low incoming

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• mid incoming 

• high incoming 

2. Weather Conditions 

• Wind speed will be listed as both direction and approximate speed in mph which will 

be converted to metric by computer at a later time. example: 10-15 mph NNW 

• Cloud cover will be listed as a percent. example: 30 % cloud cover, light rain 

• Air temperature will be estimated based upon the most recently received forecast 

from the National Weather Service. example: 25.4 C 

9.5 Instrument & Equipment Maintenance 

Most of the field sampling equipment and instruments are to be used frequently enough 

that normal maintenance will be done in conjunction with pre-mobilization and 

calibration procedures. In addition, all equipment is to be cleaned and checked after each 

use and any required maintenance performed at that time. 

All non-routine repairs are to be documented on appropriate Equipment Repair Forms as 

soon as a sampling run is completed. These are to be given to the Project Manager, who 

will transfer them to the QA/QC Officer’s records as soon as corrective action has taken 

place 

In the event of a major equipment failure, options to be implemented are listed below in 

order of priority. Decisions regarding options are to be based on the highest level of data 

collection attainable as assessed by the PI present at the time and place of the failure (any 

such activities must be noted in the field notebook). 

1. Use backup equipment. Both PBS&J and other members of the HBMP study team 

will each maintain a full array of field sampling equipment (boats, GPS units, 

binoculars, etc.). All field crews will carry cellular telephones and a list of contacts 

that will provide backup sampling equipment in order to reduce downtime due to 

equipment failure. 

2. Postpone sampling if appropriate backup equipment is not readily available, until 

repairs are made to the equipment or other sampling arrangements can be made. 

3. Record observations using the best available equipment and methods. 

4. Invalidate data. Repeat or report as N/A any invalidated data.

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Table 9.1. Avifauna Survey Equipment Checklist 

Name: 

Equipment Type 

Date: 

 

 

 

 

 

 

 

 

 

 

 

 

Data record forms 

Binoculars (7-9 power) 

Lens cleaner 

Field Guides (other references) 

Spotting scope (20 power or greater) 

Tripod 

Boat 

Hand-held Tally Counter 

Compass or Geographic Positioning System Unit 

Survey Area Infrared Aerial Photographs and other maps 

Sunscreen 

Insect repellent 

Note: Required field guides include Peterson Field Guide: Birds (eastern) or National Geographic’s Field 

Guide to Birds of North America, and Peterson Field Guide: Advanced Birding. See endnotes for other 

reference documents.

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Table 9.2. Alafia River (Banks) Bird Survey Data Collection Form: Sample 

ID# Species Name Station 1 Station 2 Station 3 Station 4 

Time on: Time on: Time on: Time on: 

Sample Time Record 

Time off: Time off: Time off: Time off: 

Time on: (dup) Time on: (dup) Time on: (dup) Time on: (dup) 

Time off: (dup) Time off: (dup) Time off: (dup) Time off: (dup) 

Tide Stage: Tide Stage: Tide Stage: Tide Stage: 

Envir. Condition 

Wind: Wind: Wind: Wind: 

Weather (Temp): Weather (Temp): Weather (Temp): Weather (Temp): 

1 American Avocet 

duplicate sample 

2 American Coot 


3 American Oystercatcher 


4 American Wigeon 


5 Black Skimmer 


6 Black-bellied Plover 

7 


Black-crowned Night 

Heron 


8 Black-necked Stilt 


9 Blue-winged Teal 


10 Bonaparte's Gull 


11 Brown Pelican 


12 Caspian Tern 


13 Cattle Egret 


14 Clapper Rail 


15 Common Loon 


16 Common Moorhen 


17 Common Tern 

18 


Double-crested 

Cormorant

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Table 9.3 

Foraging Survey Data Form 

Date (YY/MM/DD) ____________________ Time on ____________________ Time off ____________________ 

Species 

Duratio 

n (sec) 

Capture 

s 

Lat 

(deg) 

Lat 

(min) 

Lon 

(deg) 

Lon 

(min) Tide Wind Temp 

Cloud 

cover 

Notes

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Alafia Banks 

Figure 9.1. The location of the Alafia Banks monitoring sites.

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Figure 9.2. Organization of the Water-Dependent Birds Element. 

Project 

Management 

Bob Woithe, Ph.D. 

Principal 

Investigator 

Tom Davidowicz 

Tim Mann 

Field Observations 

Tom Davidowicz 

Tim Mann 

Jeff Winter 

Samantha Symon 

Data Analyses 

& Reporting 

Tom Davidowicz

Appendix 9-A 

Example of Equipment Repair Form

Equipment Repair Form 

Equipment description: ________________________________________________________ 

Date reported: _______________________________ 

Signature: _____________________________________ 

Problem description: 

___________________________________________________________ 

Action taken: _________________________________________________________________ 

Repair description: ____________________________________________________________ 

Date resolved: _____________________________ 

Resolved by (signature): ______________________________ 

Project Manager (signature): ___________________________

Appendix 9-B 

Bird Species Observed at the HBMP Stations Through 

September 2001

BirdID Common Name Genus Species Family Name Order Name Notes 

1 American Avocet Recurvirostra americana Recurvirostridae Charadriiformes 

2 American Coot Fulica americana Rallidae Gruiformes 

3 American Oystercatcher Haematopus palliatus Haematopodidae Charadriiformes 

4 American Wigeon Anas americana Anatidae Anseriformes 

5 Black Skimmer Rynchops niger Laridae Charadriiformes 

6 Black-bellied Plover Pluvialis squatarola Charadriidae Charadriiformes 

Black-crowned Night 

7 Heron Nycticorax nycticorax Ardeidae Ciconiiformes 

8 Black-necked Stilt Himantopus mexicanus Recurvirostridae Charadriiformes 

9 Blue-winged Teal Anas discors Anatidae Anseriformes 

10 Bonaparte's Gull Larus philadelphia Laridae Charadriiformes 

11 Brown Pelican Pelecanus occidentalis Pelecanidae Pelecaniformes 

12 Caspian Tern Sterna caspia Laridae Charadriiformes 

13 Cattle Egret Bubulcus ibis Ardeidae Ciconiiformes 

14 Clapper Rail Rallus longirostris Rallidae Gruiformes 

15 Common Loon Gavia immer Gaviidae Gaviiformes 

16 Common Moorhen Gallinula chloropus Rallidae Gruiformes 

17 Common Tern Sterna hirundo Laridae Charadriiformes 

18 

Double-crested 

Cormorant Phalacrocorax auritus Phalacrocoracidae Pelecaniformes 

19 Dunlin Calidris alpina Scolopacidae Charadriiformes 

20 Forster's Tern Sterna forsteri Laridae Charadriiformes 

21 Glossy Ibis Plegadis falcinellus Threskiornithidae Ciconiiformes 

22 Great Blue Heron Ardea herodias Ardeidae Ciconiiformes 

23 Great Egret Ardea alba Ardeidae Ciconiiformes 

24 Greater Yellowlegs Tringa melanoleuca Scolopacidae Charadriiformes 

25 Green Heron Butorides virescens Ardeidae Ciconiiformes 

26 Green-winged Teal Anas crecca Anatidae Anseriformes 

27 Herring Gull Larus argentatus Laridae Charadriiformes 

28 Hooded Meganser Lophodytes cucullatus Laridae Charadriiformes 

29 Killdeer Charadrius vociferus Charadriidae Charadriiformes 

30 Laughing Gull Larus atricilla Laridae Charadriiformes 

31 Least Sandpiper Calidris minutila Scolopacidae Charadriiformes 

32 Least Tern Sterna antillarum Laridae Charadriiformes 

33 Lesser Scaup Aythya affinis Anatidae Anseriformes 

34 Lesser Yellowlegs Tringa flavipes Scolopacidae Charadriiformes 

35 Little Blue Heron Egretta caerulea Ardeidae Ciconiiformes 

36 Long-billed Dowitcher Limnodromus scolopaceus Scolopacidae Charadriiformes 

37 Marbled Godwit Limosa fedoa Scolopacidae Charadriiformes 

38 Mottled Duck Anas fulvigula Anatidae Anseriformes 

39 Pied billed Grebe Podilymbus podiceps Podicipedidae Podicipediformes 

40 Piping Plover Charadrius melodus Charadriidae Charadriiformes 

41 Red Knot Calidris canutus Scolopacidae Charadriiformes 

42 Red-breasted Merganser Mergus serrator Anatidae Anseriformes 

43 Reddish Egret Egretta rufescens Ardeidae Ciconiiformes 

44 Redhead Aythya americana Anatidae Anseriformes 

45 Ringbilled Gull Larus delawarensis Laridae Charadriiformes 

46 Ring-necked Duck Aythya collaris Anatidae Anseriformes 

47 Roseate Spoonbill Ajaia ajaia Threskiornithidae Ciconiiformes

BirdID Common Name Genus Species Family Name Order Name Notes 

48 Royal Tern Sterna maxima Laridae Charadriiformes 

49 Ruddy Turnstone Arenaria interpres Scolopacidae Charadriiformes 

50 Sanderling calidris alba Scolopacidae Charadriiformes 

51 Sandwich Tern Sterna sandvicensis Laridae Charadriiformes 

52 Semipalmated Plover Charadrius semipalmatus Charadriidae Charadriiformes 

53 Semipalmated Sandpiper Calidris pusilla Scolopacidae Charadriiformes 

54 Short-billed Dowitcher Limnodromus griseus Scolopacidae Charadriiformes 

55 Snowy Egret Egretta thula Ardeidae Ciconiiformes 

56 Snowy plover Charadrius alexandrinus Charadriidae Charadriiformes 

57 Solitary Sandpiper Tringa solitaria Scolopacidae Charadriiformes 

58 Sora Porzana carolina Rallidae Gruiformes 

59 Spotted Sandpiper Actitus macularia Scolopacidae Charadriiformes 

60 Tricolored Heron Egretta tricolor Ardeidae Ciconiiformes 

61 Western Sandpiper Calidris mauri Scolopacidae Charadriiformes 

62 Whimbrel Numenius phaeopus Scolopacidae Charadriiformes 

63 White Ibis Eudocimus albus Threskiornithidae Ciconiiformes 

64 White Pelican Pelecanus erythrorhynchos Pelecanidae Pelecaniformes 

65 Willet Catoptrophorus semipalmatus Scolopacidae Charadriiformes 

66 Wood Stork Mycteria americana Ciconiidae Ciconiiformes 

67 

Yellow-crowned Night 

Heron Nyctanassa violacea Ardeidae Ciconiiformes 

68 Bald Eagle Haliaeetus leucocephalus Accipitridae Falconiformes 

69 Long Billed Curlew Numenium americanus Scolopacidae Charadriiformes 

70 

71 Royal/Caspianten Sterna sp. Laridae Charadriiformes 

72 Black Tern Chlidonias niger Laridae Charadriiformes 

73 Wilson's Plover Charadrius wilsonia Charadriidae Charadriiformes 

74 Fish Crow Corvus ossifragus Corvidae 

75 Mallard Anas platyrhynchos Anatidae Anseriformes 

70 was previously 

given as sandpipers; 

changed to 76 

76 Scolopacldae Scolopacldae Charadriiformes sandpipers 

77 Least Bittern Ixobrychus exilis Ardeidae Ciconiiformes 

78 Black Vulture Coragyps atratus Cathartidae Ciconiiformes 

79 Boat-tailed Grackle Quiscalus major Icteridae 

80 Turkey Vulture Cathartes aura Cathartidae Ciconiiformes 

82 Laridae Laridae Charadriiformes gulls/terns 

83 Northern Shoveler Anas clypeata Anatidae Anseriformes 

84 Anhinga Anhinga anhinga Anhingidae Ciconiiformes 

Immature Ibis (glossy or 

85 white) Threskiornithidae Ciconiiformes

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This section describes the standard protocols, methods and procedures to be utilized during 

the vegetation monitoring elements of the HBMP. 

Habitat indicators, like biotic indicators, typically have indirect relationships to changes in 

freshwater inflow that are mediated by physical and chemical changes. The changes resulting 

from these relationships are generally manifested over a long term time scale. For example, 

changes in vegetation and sediment distribution patterns in response to small changes in 

freshwater inflows are likely to occur over a period of several years. Table 10.1 provides a 

summary of relative critical indicators, associated units of measure, and the primary sources 

of variability for each. 

During the initial design phase, the HBMP Focus Group agreed to the importance of tracking 

both submergent and emergent aquatic vegetation within the Alafia River in assessing 

potential long-term changes in habitat characteristics. The inclusion of quantitative 

vegetation monitoring in the lower Hillsborough River and Palm River/TBC systems was not 

recommended by the Focus Group because these areas lack natural vegetation. 

Based on submergent vegetation data collected in the Alafia River during initial HBMP 

implementation, the District approved a request in October 2001 to modify the frequency of 

submerged aquatic vegetation surveys in the Alafia River from once per year to once every 

five years. 

10.2 Organization and Personnel 

At a minimum, field staff should have degrees in an appropriate field of biology or 

environmental science, and at least two years of experience that include field 

identification/delineation of vegetation in Florida. Senior and supervisory staff should have 

significant additional specific relative experience and/or education. Figure 10.1 outlines the 

organizational responsibilities of the PBS&J staff with regard to this HBMP monitoring 

element. 

Field sampling will be conducted by at least two PBS&J staff whenever possible. Field 

crews will be trained in all sampling procedures and will have copies of all the pertinent 

chapters of the HBMP Project Specific QA/QC Document for consultation in the field. An 

annual training workshop will be held to review all sampling protocols, as well as field and 

sampling procedures. This workshop will be held in conjunction with an annual review of 

the HBMP field safety program. Field sampling will occur in the late summer or fall.

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It is imperative that the methods used by HBMP vegetation monitoring personnel correspond 

to those contained in this portion of the QA/QC Plan. These standardized monitoring 

procedures, interpretation techniques, data recording methods, etc. will ensure that the 

collected data can be combined or compared, as appropriate. 

Members of the vegetation monitoring team for the HBMP must read, understand and follow 

all steps in each procedure outlined below. If questions or suggestions arise, the Project 

Manager must be consulted for clarification before any further actions can be taken. Under 

no circumstance should a procedure or steps within a procedure be omitted or modified. 

10.3 HBMP Vegetation Monitoring Components 

The HBMP design calls for the following three different data collection activities for the 

habitat/vegetation element. 

• In the Alafia River only - Species composition and abundance of emergent and 

submerged aquatic vegetation at fixed stations within the marine/brackish and 

brackish/freshwater transition zones. 

• Aerial photographic interpretation and mapping to estimate total cover of vegetation 

types in each of the four reporting units. 

• Shoreline survey to estimate areal extent, and upstream/downstream limits of emergent 

aquatic vegetation communities in each of the four reporting units. 

Vegetation will be assessed along portions of the Alafia River where transitions in plant 

species dominance occur. Percent cover at fixed stations and the upstream/downstream 

extent of black needlerush (Juncus roemerianus) will be determined and compared with 

salinity and other data to establish baseline conditions of existing vegetation communities 

and to monitor potential changes in those communities. 

10.3.1 Alafia River Transitional Area Fixed Station Vegetation Sampling 

Vegetation in the lower Alafia River will be monitored annually at fixed stations in two 

reporting units defined as the marine/brackish transitional area, and the brackish/fresh 

transitional area. These reporting units were identified from aerial photointerpretation and 

field reconnaissance of the existing first and last occurrence of black needlerush. The 

original design called for midpoints of the marine/brackish and the brackish/fresh reporting 

units to be defined as the observed downstream and upstream last occurrence of needlerush, 

respectively. The respective reporting units were to extend 2 km upstream and 2 km 

downstream from these two midpoints, and both the marine/brackish and the brackish/fresh 

reporting units would have been 4 river-km in length. Each reporting unit was to have been 

divided into four spatial strata, each 1 km in length.

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In the initial HBMP survey (November 2001), mangroves dominated the area downstream of 

the downstream-most occurrence of needlerush. During this same survey, there was less 

than 2 km of emergent vegetation upstream of the upstream-most occurrence of needlerush. 

As a result, the downstream boundary of the marine/brackish unit was established as the 

downstream-most occurrence of needlerush. The marine/brackish reporting unit extends 4 

km upstream from this boundary. The upstream boundary of the brackish/fresh unit was 

established as the last occurrence of significant emergent vegetation. The brackish/fresh 

reporting unit extends 4 km downstream from this boundary. 

There is a 0.7-kilometer gap between the upstream boundary of the marine/brackish unit, and 

the downstream boundary of the brackish/fresh unit. This gap was treated as an intermediate 

stratum. Seven stations (70% of the ten stations used in the 1 km-long strata) were 

established in the intermediate stratum to give complete fixed-station coverage throughout 

the emergent vegetation areas of the lower Alafia River. 

Species composition and abundance will be measured at fixed stations for emergent 

vegetation (annually) and randomly selected stations or a series of transects for submerged 

vegetation (every five years). These efforts will occur only on the lower Alafia River. The 

field data form used for vegetation sampling is shown in Appendix 10-A, Exhibit 10.1. 

Station locations are shown in Appendix 10-C. The HBMP vegetation sampling will follow 

the format detailed below. In order to minimize observer bias, sampling personnel will carry 

copies of the previous sampling results in the field. Before recording observations, field 

sampling personnel will compare current observations to results from the previous sampling 

efforts. Before recording observations that are different from previous sampling results, 

personnel will make conscious decisions that the differences could not be the result of 

different cover interpretations by previous observers instead of real differences in vegetation 

cover. 

Marine/Brackish Reporting Unit – beginning at downstream edge of needlerush 

distribution upstream 4 km: 

1) 4 strata – each 1-km long. 

2) 36 permanent sample stations within the unit for emergent vegetation 

3) 4 randomly selected transects (each a minimum of 100 meters long and 

located in areas with less than 2-meter water depth) within the unit for 

submerged aquatic vegetation (SAV) using a submerged video camera. If 

conditions prevent using a video camera, then 36 randomly selected sample 

stations will be monitored within the unit for submerged aquatic vegetation. 

SAV stations will be located in areas with less than 2-meter water depth. 

4) Emergent stations were established, marked, and GPS-located in Year 1. 

5) SAV transects/stations are randomly selected each year they are sampled 

(sampled once every 5 years). 

6) For emergent vegetation stations, measure species present and percent cover 

by dominant species.

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7) Relative percent cover of emergent vegetation will be calculated as a 

database procedure from reported measurement. Percent cover will be 

recorded using the Braun-Blanquet scale (Table 10.2, Braun-Blanquet 1932). 

8) Fixed station data from the previous year will be carried in the field and the 

observer will determine if the observed coverages are different from those 

recorded the previous year. If the coverages are different, the new values 

should be recorded. If they are not, the previous year's values should be 

recorded. This procedure is designed to minimize observer bias. 

9) For SAV, record species present and estimate total coverage if possible. 

10) A 4.0 m 2 quadrat will be used for emergent vegetation. The quadrat will be 

centered on the permanent station marker and oriented so that its sides are 

parallel with the North-South and East-West compass directions 

11) A 1.0 m 2 quadrat will be used for SAV quadrat (for random station 

sampling). The quadrat orientation will be random. 

12) Interstitial soil salinity will be measured in the vicinity of each fixed station 

using a refractometer (a hole is dug and water that fills the hole is placed in 

the refractometer). 

Brackish/Fresh Reporting Unit – 4 km long river segment beginning on upstream edge 

of Juncus distribution heading downstream. All procedures outlined above will be 

utilized. 

Unidentified species will be collected and sent to the University of South Florida for species 

verification. Identification of emergent aquatic vegetation (EAV) species will follow that of 

Godfrey and Wooten (1981). In addition to EAV at marsh quadrats, interstitial salinities will 

be measured using a Reichert refractometer. 

10.3.2 Floodplain Vegetation Polygon Mapping 

False-color infrared aerial photographs will be provided by Tampa Bay Water in electronic 

format. These will be supplemented with blueline aerial photographs available from 

Hillsborough County and the Florida Department of Transportation. 

The boundaries of floodplain vegetation associated with HBMP reporting units will be 

delineated by photointerpretation and field mapping. The resulting polygons will be 

digitized to create GIS coverage for each reporting unit. Inter-annual changes in the area and 

extent of major vegetation associations in the reporting units will be calculated by comparing 

the association boundaries over time. 

Aerial photographs with a scale of 1 inch = 200 ft. or larger will be used for the field 

mapping efforts. Year 2 and later photointerpretation and field mapping will use aerial 

photographs with overlays of mapping polygons from the previous mapping event. The 

observed vegetation-association boundaries should be compared with the previous event’s 

boundary to determine whether the boundary has shifted. Changes in vegetation boundaries 

will be made by creating a copy of the previous event’s coverage and editing only those

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polygons with changed boundaries or shapes. This procedure is designed to minimize 

observer bias. 

10.3.3 Linear Vegetation Mapping and Shoreline Surveys 

The field codes used in the mapping effort are given in Table 10.3. This effort will occur in 

all four HBMP reporting units. Aerial photographs with a scale of 1 inch = 200 ft. or larger 

will be used for the mapping. Year 2 and later mapping will use aerial photographs with 

overlays of mapping polygons from the previous survey period (completed every five years). 

Estimation of the first and last occurrence of vegetation community indicator species (e.g., 

Juncus) for each monitoring event will be based on the population occurrence of each 

species rather than the occurrence of individual plants of each species. This will be 

accomplished by visual inspection and identification of plant species from a boat. The falsecolor 

infrared aerial photography described above will be used in the field to assist in the 

location of the first and last occurrences of the populations of the conspicuous indicator 

species once the initial survey is completed and the photography is ground-truthed. A 

photographic map key and the photographic record of the identified locations of the 

conspicuous indicator species will be prepared and digitized to create a GIS layer of first and 

last occurrence and the vegetation associations that border the river. Inter-annual changes in 

first and last occurrence and the extent of major vegetation associations in the reporting units 

will be calculated by comparing the association boundaries over time. 

10.4 GIS Database 

Polygon and linear mapping will use a GIS database. The following section describes data 

management conventions for this database. 

10.4.1 Metadata 

GIS staff should use the ArcView metadata extension (metadata2.avx) to document all new 

shape files. Minimum metadata includes the creation date, source and projection. The 

Arc/INFO 8 metadata feature should be used to document new coverages. 

10.4.2 File Naming 

File and Directory names for GIS must not contain spaces. A mnemonic name that hints at 

the contents is best. Always keep a backup of the previous version of a shape file, in case of 

corruption or error. The user should either make a copy and put a version number at the end 

or save a copy of the original with the suffix “BK” added to the file name. 

10.4.3 Directory Structure 

The object of structuring directories is to keep ALL files used on a project in the 

project directory. This means copy graphics, or standard layers from shared directories into 

the project BEFORE linking them to an ArcView Project. Note that projects will run much

Page 6 

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April 2008 


faster and take less space if you clip a shapefile to the largest project map extent, rather than 

store and constantly redraw a state-wide coverage. 

Project Directories. 100909.10_NewProj is a sample standard project directory name. 

Project Subdirectories. The identifier “PM” designates Project Management files. The 

number of subdirectories used is at the discretion of the individual. Include the project 

control plan here as well as in hard copy in the project file. Common subdirectories include: 

• AvProj = ArcView Project files 

• Themes = All Themes: shape files, graphics, image files, geodatabases, and database 

files referenced in ArcView. If you have to send anything but the Avproj and Themes 

directory to move the project elsewhere, you have not followed the rule. 

Also, regarding all .avl files describing a theme legend - The standard legend for a 

shapefile should be stored along with the shape file and have the same name. The 

preferred choice is to put all files directly into Themes -- no subdirectories. This system 

makes it simple to re-map the .apr file to another drive or directory. 

Include Metadata files with the same name as the shape file. The arcview metadata 

extension gives them the .met extension. For coverages and personal geodatabases, the 

metadata should be included in the same directory as coverage directory or within the 

geodatabase. 

• Database = Access and other Databases that are not geodatabases, and not themes. 

• Methodology = Any innovative project methodology that a new user would need to 

know, OR that would be useful on similar projects in the future. 

• Reports = Project Reports 

• Exports = Exported Print files, JPEGS, WMF’s, etc. 

• Outside = All outside data other than standard sources. Place data from the client or a 

sub-consultant in a subdirectory indicating the source. DO NOT map ArcView projects 

to these files. 

Copy any data used in ArcView to the Themes directory. If outside data are too large to 

store a reference copy in Outside, create a directory placeholder in Outside and put in a 

readme.doc file referencing either a CD in the project file, or Read Only files in the Themes 

directory.

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10.4.4 Map Standards 

All maps going to external sources must have: 

• North Arrow, Scale and Legend, 

• Date, 

• Sources, 

• APR File Name with full path, and 

• Layout name. 

NOTE. This implies that if someone calls for more copies, or revised copies, anyone should 

be able to open the Project File, pull up the Layout, and see the same layout that is in the 

hard copy. That means creating a separate view and separate Layout for each map. There is 

a “copy layout” extension on the Internet, or group the contents of a layout, cut and paste 

into a new layout, then ungroup. 

10.4.5 Map Revisions 

When revising maps, the requested changes should be marked on the original map with 

highlighter or other obvious markings. Cross off the revisions with a different color 

highlighter as they are completed. 

10.4.6 Map Review 

Always have someone else review maps before sending them out. 

Transmittals to outside clients should always pass through the Project Manager, unless the 

PM designates otherwise. 

10.4.7 Automated Verification Procedures 

In addition to the standard visual checks of your work to confirm that it adheres to the 

relevant standards, always be on the lookout for new verification procedures. These 

typically would involve referencing to an outside source, or an earlier version, or checking 

for known data ranges. 

When dealing with many files, check to make sure file counts are consistent and no files 

have been left out. 

Check data for outliers. If the reasonable range for an attribute is known, check for data 

outside the range. 

Always build verification into data entry forms. Either limit users to selecting from a list, or 

put verification limits on the field.

Page 8 

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10.4.8 Electronic submittal standards 

Electronic Submittals should always be on a CD labeled with a permanent marker or label 

maker. The label should contain: PROJECT-DATA-DATE-CONTACT-COMMENTS.

Page 9 

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Table 10.1 Vegetation Indicators, Units of Measure and Sources of Variability 

Critical Indicator Units of Measure Potential Sources of Variability 

Emergent Vegetation 


- Abundance 


- Areal Extent 

Submerged Vegetation 


- Abundance 


- Areal Extent 

species % comp. 

no./unit area 

river km 

acres 

species % comp. 

no./unit area 

river km 

acres 

depth, salinity, sediment grain size, 

sediment organic matter, velocity, exotics 

depth, light transmission, salinity, 

sediment grain size, velocity 

Table 10.2 The Braun-Blanquet Vegetation Classification Scale 

Rating Number Of Plants Area Occupied 

+ Sparse or very sparse Very small 

1 Plentiful Small 

2 Very numerous 10-25% 

3 Any number 25-50% 

4 Any number 50-75% 

5 Any number >75%

Page 10 

Section 10.0 

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Table 10.3 Shoreline and Wetland Mapping Classification System 

FLUCCS 

Code 

Shore Type 

Description 

4000 Upland Forests Natural forest areas with at least 10% canopy closure 

5411 Tidal Streams/Creeks Must be less than 1 mile wide 

5413 Natural Non-Vegetated Natural shoreline with no vegetation 

6100 Wetland Hardwood Forests 

Forested freshwater wetlands with at least 10% canopy closure, 

with at least 66% relative cover by hardwoods, but where no tree 

species achieves 66% relative cover 

6120 Mangrove Swamp Can be red or black mangroves with at least 10% canopy closure 

6200 Wetland Coniferous Forests 

6300 Mixed Wetland Forest 

Forested freshwater wetlands with at least 10% canopy closure, 

with at least 66% relative cover by conifers, but where no tree 

species achieves 66% relative cover 

Forested freshwater wetlands with at least 10% canopy closure, but 

where no tree species or group (hardwood or conifer) achieves 66% 

relative cover 

6400 

Vegetated Non-Forested 

Wetland 

Wetlands with less than 10% canopy closure by trees, where no 

species comprises 66% or more of the relative aerial cover 

6411 Sawgrass At least 66% relative aerial coverage by sawgrass 

6412 Cattail 

At least 66% relative cover by cattail and less than 10% aerial cover 

by needlerush 

6421 Cordgrass At least 66% relative cover by Spartina 

6422 Needlerush 

6424 Needlerush/Cattail 

6425 Needlerush/Leather Fern 

6426 Mixed Saltwater Marsh 

At least 66% relative cover by needlerush and less than 10% aerial 

cover by leatherfern 

At least 66% relative aerial coverage by needlerush and cattail 

combined and at least 10% relative aerial coverage each species 

individually 

At least 66% relative aerial coverage by needlerush and leather fern 

combined and at least 10% relative aerial coverage each species 

individually. 

Non-forested saltwater wetlands where no species comprises 66% 

or more of the relative aerial cover 

6428 Common reed At least 66% relative aerial coverage by Phragmites 

9110 

Submerged Aquatic 

Vegetation (SAV) 

Presence of SAV, including seagrasses 

9121 Attached algae Presence of attached marine or freshwater algae

Page 11 

Section 10.0 

April 2008 


Vegetation Studies 

Bob Woithe 

Shoreline 

Survey 

Aerial 

Photography 

Alafia 

Transition Zones 

Emergent Vegetation 

Areal Extent & Limits 

B. Woithe 

Field Staff 

Estimations – Total 

Coverage By Type 

B. Woithe 

P. Latham 

K. Anamisis 

Emergent & 

Submergent 

B. Woithe 

Field Staff 

Figure 10.1 Organization of Vegetation Elements

Appendix 10-A 

Example of Vegetation Monitoring Forms

Exhibit 10.1 Fixed-station vegetation sampling data form 

Notebook #: 100909-# TBW HBMP Page # _______ 

Reporting Unit: ____________ Stratum: _______________ Station # _____________ 

Lat degrees 27 minutes __________ Long deg__________ 82 minutes____________ 

Date: _________ _________ (yy/mm/dd) Time On Station (EST) ________________________ 

Species Br.-Bl. Cover Species Br.-Bl. Cover 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

Date: _________________ (yy/mm/dd) Time On Station (EST) _______________ 

Tide Stage: _________________ 

Interstitial Salinity (ppt) __________________ 

Field Notes: _______________________________________________________________________ 

__________________________________________________________________________________ 

__________________________________________________________________________________ 

Reporting Unit: ____________ Stratum: _______________ Station # _____________ 

Lat degrees 27 minutes __________ Long deg__________ 82 minutes____________ 

Date: _________ _________ (yy/mm/dd) Time On Station (EST) ________________________ 

Species Br.-Bl. Cover Species Br.-Bl. Cover 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

_____________________________ ________ ____________________________ ______ 

Date: _________________ (yy/mm/dd) Time On Station (EST) _______________ 

Tide Stage: _________________ 

Interstitial Salinity (ppt) __________________ 

Field Notes: _______________________________________________________________________ 

__________________________________________________________________________________ 

__________________________________________________________________________________ 

Braun Blanquet 

Rating Number of Plants Area Occupied Rating Number of Plants Area Occupied 

+ Sparse or very sparse very small 3 Any number 25-50% 

1 Plentiful Small 4 Any number 50-75% 

2 Very numerous 10-25% 5 Any number >75%

Appendix 10-B 

Plant Species Observed at HBMP Fixed Stations 

Through 2004

HBMP Master Vegetation Species List 

Species# Genus species Family Common Name 

0 Bare ground -9999 -9999 Bare ground 

1 Typha angustifolia Typhaceae Narrow leaf cattail 

2 Crinum americanum Agavaceae String lily 

3 Juncus roemerianus Juncaceae Needle rush 

4 Cladium jamaicense Cyperaceae Sawgrass 

5 Panicum repens Poaceae Torpedo grass 

6 Acrostichum aureum Adiantaceae Golden leather fern 

7 Rhizophora mangle Rhizophoraceae Red mangrove 

8 Avicennia germinans Verbenaceae Black mangrove 

9 Ruppia maritima Zosteraceae Widgeon grass 

10 Brachiaria purpurascens Poaceae Para grass 

11 Solidago spp. . .

Appendix 10-C 

HBMP Alafia River Vegetation Monitoring Fixed 

Station Locations

Veg_Stratum 

HBMP Fixed Vegetation Monitoring Station Locations 

Veg_Station 

Latitude_Degrees 

Latitude_Minutes 

Longitude_Degrees 

Longitude_Minutes 

Equivalent River Centerline 

Station 

1 1 27 52.253 82 18.647 AR510362 10.362 

1 2 27 52.259 82 18.660 AR510334 10.334 

1 3 27 52.243 82 18.678 AR510306 10.306 

1 4 27 52.236 82 18.825 AR510059 10.059 

1 5 27 52.226 82 18.825 AR510054 10.054 

1 6 27 52.226 82 18.825 AR510054 10.054 

1 7 27 52.197 82 18.861 AR510000 10.000 

1 8 27 52.191 82 18.874 AR509976 9.976 

1 9 27 52.156 82 18.879 AR509945 9.945 

2 1 27 51.941 82 18.803 AR509491 9.491 

2 2 27 51.927 82 18.809 AR509458 9.458 

2 3 27 51.910 82 18.816 AR509420 9.420 

2 4 27 51.902 82 18.857 AR409329 9.329 

2 5 27 51.906 82 18.864 AR409317 9.317 

2 6 27 51.916 82 18.871 AR409317 9.317 

2 7 27 51.984 82 18.943 AR409131 9.131 

2 8 27 51.991 82 19.123 AR408799 8.799 

2 9 27 51.990 82 19.132 AR408784 8.784 

3 1 27 52.069 82 19.248 AR408583 8.583 

3 2 27 52.132 82 19.329 AR408420 8.420 

3 3 27 52.133 82 19.336 AR408406 8.406 

3 4 27 52.191 82 19.477 AR408143 8.143 

3 5 27 52.192 82 19.487 AR408127 8.127 

3 6 27 52.108 82 19.573 AR407983 7.983 

3 7 27 52.157 82 19.639 AR407871 7.871 

3 8 27 52.066 82 19.679 AR407799 7.799 

3 9 27 52.063 82 19.688 AR407782 7.782 

4 1 27 51.986 82 19.808 AR407537 7.537 

4 2 27 51.973 82 19.950 AR407240 7.240 

4 3 27 51.930 82 20.012 AR407112 7.112 

4 4 27 51.856 82 20.185 AR306787 6.787 

4 5 27 51.849 82 20.209 AR306744 6.744 

4 6 27 51.763 82 20.153 AR306843 6.843 

4 7 27 51.755 82 20.168 AR306817 6.817 

4 8 27 51.755 82 20.182 AR306793 6.793 

4 9 27 51.831 82 20.266 AR306643 6.643 

5 1 27 51.431 82 21.038 AR305054 5.054 

5 2 27 51.435 82 21.087 AR304973 4.973 

5 3 27 51.555 82 20.590 AR305988 5.988 

5 4 27 51.548 82 20.595 AR305970 5.970 

5 5 27 51.529 82 20.616 AR305900 5.900 

5 6 27 51.518 82 20.605 AR305942 5.942 

5 7 27 51.507 82 20.620 AR305887 5.887 

6 1 27 51.507 82 20.630 AR305855 5.855 

6 2 27 51.495 82 20.652 AR305795 5.795 

6 3 27 51.544 82 20.785 AR305495 5.495 

River Kilometer

Veg_Stratum 

Veg_Station 

Latitude_Degrees 

Latitude_Minutes 

Longitude_Degrees 

Longitude_Minutes 

Equivalent River 

Centerline Station 

River Kilometer 

6 5 27 51.549 82 20.817 AR305434 5.434 

6 6 27 51.542 82 20.843 AR305384 5.384 

6 7 27 51.528 82 20.844 AR305382 5.382 

6 8 27 51.431 82 21.038 AR305054 5.054 

6 9 27 51.435 82 21.087 AR304973 4.973 

7 1 27 51.509 82 21.354 AR204525 4.525 

7 2 27 51.626 82 21.367 AR204504 4.504 

7 3 27 51.616 82 21.384 AR204476 4.476 

7 4 27 51.578 82 21.398 AR204453 4.453 

7 5 27 51.562 82 21.420 AR204416 4.416 

7 6 27 51.551 82 21.424 AR204410 4.410 

7 7 27 51.545 82 21.423 AR204411 4.411 

7 8 27 51.498 82 21.598 AR204121 4.121 

7 9 27 51.492 82 21.631 AR204067 4.067 

8 1 27 51.534 82 21.811 AR203767 3.767 

8 2 27 51.548 82 21.834 AR203725 3.725 

8 3 27 51.535 82 22.048 AR203349 3.349 

8 4 27 51.535 82 22.073 AR203308 3.308 

8 5 27 51.326 82 22.264 AR202987 2.987 

8 6 27 51.330 82 22.265 AR202985 2.985 

8 7 27 51.343 82 22.264 AR202987 2.987 

8 8 27 51.257 82 22.202 AR203093 3.093 

8 9 27 51.260 82 22.205 AR203088 3.088 

9 1 27 51.364 82 22.401 AR202759 2.759 

9 2 27 51.354 82 22.417 AR202733 2.733 

9 3 27 51.336 82 22.504 AR202590 2.590 

9 4 27 51.356 82 22.501 AR202595 2.595 

9 5 27 51.319 82 22.600 AR202431 2.431 

9 6 27 51.622 82 22.478 AR202632 2.632 

9 7 27 51.629 82 22.485 AR202620 2.620 

9 8 27 51.639 82 22.553 AR202509 2.509 

9 9 27 51.651 82 22.687 AR102288 2.288

Page 1 of 2 

Section 11.0 

April 2008 


11.0 References and Relevant Literature 

Braun-Blanquet, J. 1932. Plant Sociology. Oxford University Press. Oxford, UK. 

Campbell Scientific. 1997. CR10X Measurement and Control Module Operator’s 

Manual. Campbell Scientific, Inc. Logan, UT. 

Campbell Scientific. 2007. LoggerNet Instruction Manual. Campbell Scientific, Inc. 

Logan, UT. 

Cochran, W.G. 1977. Sampling Techniques. 3 rd Edition. John Wiley & Sons. New 

York, NY. 

Cochran, W.G., F. Mosteller, and J.W. Tukey. 1954. Principles of sampling. Journal of 

the American Statistical Association 49:13-35. 

Furness, R.W. and J.J.D. Greenwood (eds.). 1993. Birds as Monitors of Environmental 

Change. Chapman and Hall. London, England. 

Godfrey, R.K., and J. W. Wooten. 1981. Aquatic and Wetland Plants of the 

Southeastern United States. University of Georgia Press. Athens, GA. 

Goss-Custard, J.D. 1967. The Winter Feeding Ecology of the Redshank Tringa totanus. 

Ibis 111: 338-356. 

Grant, P.J. 1999. Gulls: A Guide to Identification. Academic Press. San Diego, CA. 

Hayman, P., J. Marchant and J. Prater. 1986. Shorebirds: An Identification Guide 

Houghton Mifflin. Boston, MA. 

Hunt, D.T.E, and A.L. Wilson. 1986. The Chemical Analysis of Water: General 

Principles and Techniques. 2 nd Edition. Royal Society of Chemistry, 

London, England. 

Kaufman, K. 1990. A Field Guide to Advanced Birding. Peterson Field Guide Series. 

Houghton Mifflin Company. Boston, MA. 

Kirchner, C.J. 1983. Quality Control in Water Analysis. Environmental Science and 

Technology 17(4):174A-181A. 

Moreira, Francisco. 1993. Winter Feeding Ecology of Black-tailed Godwits. Ibis 136: 

349-355.

Page 2 of 2 

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April 2008 


Morgan, B.J.T. and P.M. North (eds.). 1985. Statistics in Ornithology. Springer-Verlag. 

Berlin, Germany. In Billinger, D., et. al. (eds.). Volume 29 of Lecture 

Notes in Statistics. 

National Research Council. 1990. Managing Troubled Waters. National Academy 

Press. Washington, DC. 

PBS&J. 2000. Tampa Bypass Canal/Alafia River Water Supply Projects 

Hydrobiological Monitoring Program. Final Reports prepared for Tampa 

Bay Water. PBS&J. Tampa, FL. 

PBS&J. 2001. Tampa Bypass Canal/Alafia River Water Supply Projects 

Hydrobiological Monitoring Program – Water Year 2000 Data Report. 

Prepared for Tampa Bay Water. PBS&J. Tampa, FL. 

Scott, S. L. 1989. Field Guide to the Birds of North America. 2 nd Edition National 

Geographic Society. New York, NY. 

Southern Analytical Laboratories, Inc. 2001. Quality Manual for Southern Analytical 

Laboratories, Inc. Southern Analytical Laboratories, Inc. Oldsmar, FL. 

Stanley, T.W., and S.S. Verner. 1985. The U.S. Environmental Protection Agency’s 

Quality Assurance Program. In: J.K. Taylor and T.W. Stanley (eds.) 

Quality Assurance for Environmental Measurements. American Society 

for Testing Materials. Philadelphia, PA. 

Stevens Water Monitoring Systems. 1999. SDI Encoder Instruction 91196. Stevens 

Water Monitoring Systems, Inc. Beaverton, OR. 

Stevenson, H.M. and B.H. Anderson. 1994. The Birdlife of Florida. University Press of 

Florida. Gainesville, FL. 

Summers, J.K., and G. Maddox. 1999. Design of the Status Monitoring Network. In: 

Overview of the Florida Department of Environmental Protection’s 

Integrated Water Resource Monitoring Efforts and the Design Plan of the 

Status Network. Florida Department of Environmental Protection. 

Tallahassee, FL. 

Taylor, J.K. 1987. Quality Assurance of Chemical Measurements. Lewis Publishers. 

Boca Raton, FL. 

YSI. 1999. 6-Series YSI Environmental Operations Manual. YSI, Inc. Yellow Springs, 

OH.

quality assurance and quality control plan - Tampa Bay Water

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