Two novel triterpenoid saponins, colqueleganoids A (1) and B (2), with the first methyl-30 incorporated 6/6/6/6-cyclized carbon skeleton (named colquelegane), were isolated from the root of Colquhounia elegans. Their structures including absolute configuration were determined by spectroscopic methods and X-ray crystallographic analyses. Interestingly, both compounds significantly enhanced TNF-α production and 1 also increased the IL-6 production in RAW264.7 macrophages stimulated with lipopolysaccharide (LPS), suggesting their potential application as immunostimulants in immunotherapy and vaccination.

中文翻译：

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来自线虫根的带有甲基 30 环化作用的免疫刺激 6/6/6/6 四环三萜皂苷

从Colquhounia elegans的根中分离出两种新型三萜皂苷，colqueleganoids A ( 1 ) 和B ( 2 )，其中第一个甲基30结合了6/6/6/6-环化碳骨架（命名为colquelegane）。它们的结构包括绝对构型是通过光谱方法和 X 射线晶体学分析确定的。有趣的是，这两种化合物都显着增强了 TNF-α 的产生，1还增加了用脂多糖 (LPS) 刺激的 RAW264.7 巨噬细胞中 IL-6 的产生，表明它们在免疫治疗和疫苗接种中作为免疫刺激剂的潜在应用。