References
[1] A Complete English Dictionary of Medicinal Terms in Chinese Acupuncture and
Herbalism 1981- Henry Lu Chinese Foundations of Natural Health- The Academy of
Oriental Heritage, Vancouver, Canada. Images
1. nativeamericanhumorandsomeextrastandups.wordpress.com Inner Path can not take any responsibility for any adverse effects from the
use of plants. Always seek advice from a professional before using a plant medicinally. Research
Polyphenol-rich extract of Salvia chinensis exhibits anticancer activity in different
cancer cell lines, and induces cell cycle arrest at the G₀/G₁-phase, apoptosis
and loss of mitochondrial membrane potential in pancreatic cancer cells. Zhao Q, Huo XC, Sun FD, Dong RQ3.
Abstract Pancreatic cancer (PC) is one of the most aggressive types of human malignancy,
which has an overall 5-year survival rate of <2%. PC is the fourth most common
cause of cancer‑associated mortality in the western world. At present, there is
almost no effective treatment available for the treatment of PC. The aim of the
present study was to evaluate the anticancer potential of a polyphenol enriched
extract obtained from Salvia chinensis, a Chinese medicinal plant. An MTT assay
was used to evaluate the cell viability of five cancer cell lines and one normal
cell line. In addition, the effects of the extract on apoptotic induction, cell
cycle phase distribution, DNA damage and loss of mitochondrial membrane potential
(ΛΨm) were evaluated in MiapaCa‑2 human PC cells. The effects of the extract on
cell cycle phase distribution and ΛΨm were assessed by flow cytometry, using propidium
iodide and rhodamine‑123 DNA‑binding fluorescent dyes, respectively. Fluorescence
microscopy, using 4',6‑diamidino‑2‑phenylindole as a staining agent, was performed
in order to detect the morphological changes of the MiapaCa‑2 cancer cells and
the presence of apoptotic bodies following treatment with the extract. The results
of the present study demonstrated that the polyphenol‑rich extract from S. chinensis
induced potent cytotoxicity in the MCF‑7 human breast cancer cells, A549 human
lung cancer cells, HCT‑116 and COLO 205 human colon cancer cells, and MiapaCa‑2
human PC cells. The Colo 205 and MCF‑7 cancer cell lines were the most susceptible
to treatment with the extract, which exhibited increased rate of growth inhibition.
Fluorescence microscopy revealed characteristic morphological features of apoptosis
and detected the appearance of apoptotic bodies following treatment with the extract
in the PC cells. Flow cytometric analysis demonstrated that the extract induced
G0/G1 cell cycle arrest in a dose‑dependent manner. In addition, treatment with
the extract induced a significant and concentration-dependent reduction in the
ΛΨm of the PC cells.
PMID: 26165362 PMCID: PMC4581742 DOI: 10.3892/mmr.2015.4074 Mol Med Rep. 2015
Oct;12(4):4843-50. doi: 10.3892/mmr.2015.4074. Epub 2015 Jul 13. ncbi.nlm.nih.gov
Salvia
chinensis. 石
见 穿
Shí jiàn chuān Family:
Labiatae
FLAVOR: Bitter, pungent CHANNELS: Heart, Pericardium,
Lung, Stomach, Liver, Spleen