Abstract
To investigate the Ns genome donor of Leymus and to elucidate the origin of Xm genome of Leymus, Random Amplified Polymorphic DNA (RAPD) markers were used to analyze 13 Leymus accessions, together with 14 taxa from related Triticeae genera. A total of 156 clear and reproducible DNA fragments were amplified by 24 RAPD primers, among which 154 fragments (98.72%) were found to be polymorphic. The main results showed as following: (1) the Leymus species were clustered together first, and there is the closest phylogenetic relationship between L. akmolinensis and L. angustus; (2) Leymus species have Ns genome, which come from Psathyrostachys, and different Psathyrostachys species could serve as the donor for different Leymus species; (3) The partial Leymus species had a close phylogenetic relationship with Hordeum chilense, Australopyrum retrofractum, Lophyrum elongatum.
Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 31270243). We would like to specially thank the Triticeae Research Institute of Sichuan Agriculture University for providing the seeds and plants.
References
Anamthawat-Jónsson K. & B¨odvarsdóttir S.K. 2001. Genomicand genetic relationships among species of Leymus (Poaceae: Triticeae) inferred from 18S-26S ribosomal genes. Am. J. Bot. 88: 553–559.10.2307/2657053Search in Google Scholar
Aparajita S. & Rout GR. 2009. Genetic differentiation of Albizialebbeck (L.) Benth. Populations estimated by RAPD and ISSR markers. Plant Biosyst. 143: 361–368.10.1080/11263500902722683Search in Google Scholar
Dewey D R. 1976. The genome constitution and phylogeny of Elymus ambiguous. Am. J. Bot. 63: 626–634.10.1002/j.1537-2197.1976.tb11850.xSearch in Google Scholar
Dewey D R. 1984. The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae, pp. 209–280. In: Gustafson J.P. (ed.), Gene Manipulation in plant improvement. Plenum Press, New York.10.1007/978-1-4613-2429-4_9Search in Google Scholar
Ding C.B., Zhou Y.H., Yang R.W., Zhang L. & Zheng Y.L. 2005. Relationships among Pseudoroegneria species assessed by RAPD markers. Acta Prataculturae Sinica 14: 38–43.Search in Google Scholar
Doyle J J. & Doyle J L. 1990. Isolation of plant DNA from fresh tissue. Focus. 12: 13–15.10.2307/2419362Search in Google Scholar
Fan X., Sha L.N., Yang R.W., Zhang H.Q., Kang H.Y., Ding C.B., Zhang L., Zheng Y.L. & Zhou Y.H. 2009. Phylogeny and evolutionary history of Leymus (Triticeae; Poaceae) based on a single-copy nuclear gene encoding plastid acetyl-CoA carboxylase. BMC Evol. Biol. 9: 247–252.10.1186/1471-2148-9-247Search in Google Scholar PubMed PubMed Central
Guo G.Y., Yang R.W., Ding C.B., Fan X., Zhang L. & Zhou Y.H. 2014. Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers. Biologia 69: 986–993.10.2478/s11756-014-0395-3Search in Google Scholar
Liu Z.P., Chen Z.Y., Pan J., Li X.F., Su M., Wang L.J., Li H.J. & Liu G.S. 2008. Phylogenetic relationships in Leymus (Poaceae: Triticeae) revealed by nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. Mol. Phylogen. Evol. 46: 278–289.10.1016/j.ympev.2007.10.009Search in Google Scholar PubMed
L¨ove A. 1984. Conspectus of the Triticeae. Feddes Rep. 95: 425– 521.10.1002/fedr.4910950702Search in Google Scholar
Rohlf F.J. 1994. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.0. Exter Software, New York.Search in Google Scholar
Sha L.N., Fan X., Yang R.W., Kang H.Y., Ding C.B., Zhang L., Zhang L., Zheng Y.L. & Zhou Y.H. 2010. Phylogenetic relationships between Hystrix and its closely related genera (Poaceae: Triticeae) based on nuclear Acc1, DMC1 and chloroplast trnL-F sequences. Mol. Phylogen. Evol. 54: 327– 335.10.1016/j.ympev.2009.05.005Search in Google Scholar PubMed
Sha L.N., Yang R.W., Fan X., Wang X.L. & Zhou Y.H. 2008. Phylogenetic analysis of Leymus (Poaceae: Triticeae) inferred from nuclear rDNA ITS sequences. Bioch. Genet. 46: 605–619.10.1007/s10528-008-9175-5Search in Google Scholar PubMed
Shiotan I. 1968. Species differentiation in Agropyron, Elymus, Hystrix, and Sitanion. Proceedings of the 12th International Congress of Genetics. The Science Council of Japan, Tokyo, 184 pp.Search in Google Scholar
Sun G.L., Wu B.H. & Liu F. 1995. Cytogenetic and genomic relationships of Thinopyrum elongatum with two Psathyrostachys species and withLeymus secalinus Poaceae. Plant Syst. Evol. 197: 225-231.10.1007/BF00984641Search in Google Scholar
Tzvelev N.N. 1976. Zlaki SSSR (Poaceae URSS.). Nauka, Leningrad.Search in Google Scholar
Wang R R-C. & Jensen K B. 1994. Absence of the J genome in Leymus species (Poaceae: Triticeae): evidence from DNA hybridization and meiotic pairing. Genome 37: 231–235.10.1139/g94-032Search in Google Scholar PubMed
Yang R.W., Zhong M.H., Zou X.M., Ding C.B., Zhang L. & Zhou Y. H. 2011. Phylogenetic relationships between Leymus (Poaceae, Triticeae) and related diploid Triticeae species-based on isozyme and genome-specific random amplified polymorphic DNA (RAPD) markers. Plant Biosystems 146: 84– 91.10.1080/11263504.2011.589476Search in Google Scholar
Yang R.W., Zhou Y.H., Ding C.B., Zheng Y.L. & Zhang L. 2008. Relationships among Leymus species assessed by RAPD markers. Biol. Plant. 52: 237–241.10.1007/s10535-008-0052-1Search in Google Scholar
Zhang H.B. & Dvorak J. 1991. The genome origin of tetraploid of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. Am. J. Bot. 78: 871–884.10.1002/j.1537-2197.1991.tb14491.xSearch in Google Scholar
Zhang H.Q. & Zhou Y.H. 2009. Genetic relationships among Hystrixpatula, H. duthiei and H. longearistata according to meiotic studies and genome-specific RAPD assay. Biol. Plant. 53: 45–52.10.1007/s10535-009-0007-1Search in Google Scholar
© Institute of Botany7, Slovak Academy of Sciences
