In Vitro PLBs organogenesis of Phalanopsis to carbohydrate sources under LED lights

H. Mehraj

Throughout of this study, our vision was to find out the impact of LED and effective carbohydrate source for the in vitro regeneration of the PLBs (protocorm-like bodies) in Phalaenopsis ‘Fmk02010’. We allowed fifteen LED treatments with three carbohydrate sources in our study. Performance of PLBs production was influenced diversely at different carbohydrate source under different LED. We found sucrose as the best carbohydrate source when it was placed under red + white LED for the in vitro organogenesis of PLBs i.e., mean number of PLBs (54.13) and their fresh weight (0.109 g) followed by white florescent tube (control) in Phalaenopsis in culture medium. On the other hand, trehalose carbohydrate source showed best performance under blue + white LED in terms of number of PLBs (36.33). Organogenesis of PLBs in culture medium was very poor in the carbohydrate source maltose than sucrose and trehalose. The result of this study suggested using sucrose as carbohydrate source and putting under red + white LED (chronologically half of the entire culture period) for the better PLBs regeneration of Phalaenopsis ‘Fmk02010’.

In vitro PLBs organogenesis of Phalaenopsis to carbohydrate sources under LED lights Hasan Mehraj1,2,¶ Md. Meskatul Alam2, Kazuhiko Shimasaki2, and Sultana Umma Habiba1 1The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan; 2Faculty of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8520, Japan ¶Presenting and corresponding author: Hasan Mehraj Corresponding author e-mail: hmehraj02@yahoo.com Abstract Throughout of this study, our vision was to find out the impact of LED and effective carbon source for the in vitro regeneration of the PLBs (Protocorm-like bodies) in Phalaenopsis ‘Fmk02010’. We allowed fifteen LED treatments with three carbon sources in our study. The performance of PLBs production was influenced differently at different carbon source under different LED. We found that sucrose was the best carbon source when it was placed under red + white LED for the in vitro organogenesis of PLBs i.e., mean number of PLBs (54.13) and their fresh weight (0.109 g) followed by white florescent tube (control) in Phalaenopsis in culture medium. On the other hand, trehalose showed best performance under blue + white LED in terms of number of PLBs (36.33). Organogenesis of PLBs in culture medium was very poor in the carbon source maltose than sucrose and trehalose. The result of this study suggested using sucrose as carbon source and putting under red + white LED (chronologically half of the entire culture period) for the better PLBs regeneration of Phalaenopsis ‘Fmk02010’. Key words: protocorm-like bodies, regeneration, light-emitting diode, sucrose, trehalose, maltose Introduction Media ingredients are the key factor for the successful PLBs regeneration by plant tissue culture. Plant tissue culture media generally have salt, mineral, vitamins, growth regulators and water for orchid tissue culture (Murdad et al., 2010) but one of the most important component is the carbon source to supply energy (Al-Khateeb, 2008). There are many carbon sources like sucrose, fructose, glucose, trehalose, maltose, and sorbitol (Liu et al., 2006; Tokuhara and Mii, 2003) used for plant tissue culture which might be in the form of simple or complex sugars (Akter et al., 2007). Sucrose is the widely used carbon source used for plant tissue culture. Light is another important factor affecting the PLB regenerations for photosynthetic and phototropic response that depends on photon flux density light quality and photoperiod of plants (Taiz and Zeiger, 1991). Fluorescent lights are commonly used but now a days LED lights have been used for In Vitro cultivations. LED is a unique type of semi conductor diode that has several technical benefits over usual light source for photosynthesis (Mitchell et al., 2012) also the user's ability to determine their spectral composition, small size, durability, long operating lifetime, wavelength specificity and relatively cool emitting surfaces. LED allows wavelengths to be matched for plant photoreceptors to influence plant morphology and metabolic composition (Bourget, 2008, Massa et al., 2008 and Morrow, 2008). Blue LED converted electrical energy to photon energy and had been reported 49% efficient in 2011 (Mitchell et al., 2012) while the predicted photosynthetic efficacy of red LED will be double than high pressure sodium lights by the year of 2020 (Pinho et al., 2012). LED lights have been previously used for PLBs organogenesis in Cymbidium finlaysonianum (Nahar et al., 2015), Dendrobium kingianum (Habiba et al., 2014a; Habiba et al., 2014b), hybrid Cymbidium (Teixeira da Silva, 2014; Kamal et al., 2014) and plantlet regeneration in gerbera (Wang, 2011). Phalaenopsis is the most important and valuable commercial orchid belonging to Orchidaceae family. It is widely accepted both as cut or pot flowers. Propagation of Phalaenopsis via vegetatively and seed is quite difficult so that tissue culture is the common need for its successful and rapid propagation. PLBs regeneration is one of the best ways to regenerate the new plant. Use of proper media compositions with optimum culture conditions is on of the significant factor to speed up micro propagation process with quality of regenerated plantlets through PLBs (Chen et al., 2000; Park et al., 2002). Growth regulators and elicitors were previously used for the PLBs regeneration of Phalaenopsis (Sultana et al., 2015a & 2015b). The purpose of this study was to search out the best carbon source and LED light for successful PLBs regeneration of Phalaenopsis ‘Fmk02010’. Materials and Methods Plant Materials and Culture Conditions: PLBs of Phalaenopsis ‘Fmk02010’ were proliferated in the modified MS (Shimasaki and Uemoto, 1990) medium by transferring to a new medium. After excision of PLB into singles, they were used for explants. Modified MS medium was supplemented with 412.5 mg/L ammonium nitrate, 950 mg/L potassium nitrate, 20 g/L carbon sources and 2.2 g/L PhytagelTM and before autoclaving pH was adjusted to 5.5- 5.8 using 1 mM 2-(N-morpholino) ethanesulfonic acid sodium salts (MES-Na). We used 30 ml culture media in each culture bottles and autoclaved at 121 °C for 15 min at 1.5 Kgf/cm2 (i.e. 14.71 Pa). PLBs in culture media: We put five single PLBs in each culture bottles and kept the culture media for PLBs regeneration under different LED lights for 60 days. Carbon sources: We used three different carbon sources for the modified MS media preparation. The carbon sources were sucrose, trehalose and maltose. LED lights: In our experiments we allowed the PLBs organogenesis under fifteen different LED light sources and these were L1 Control (White fluorescent light), L2 (Red LED), L3 (Green LED), L4 (Blue LED), L5 (White LED), L6 (Red + Green LED), L7 (Red + Blue LED), L8 (Red + White LED), L9 (Green + Blue LED), L10 (Green + White LED), L11 (Blue + White LED), L12 (Red + Green + Blue LED), L13 (Red + Blue + White LED), L14 (Red + Green + White LED) and L15 (Green + Blue + White LED). We used half of the entire culture period for double LED light and one third of the entire period for the triple LED light combinations, chronologically (according to the treatments written previously). Data Collection: We counted the total number of PLBs (including budding PLBs), shoots and roots. We also measured the length of shoot and root also the mean fresh weight of PLBs. We collected all data 60 days of culture then statistically analyzed by calculating standard error of the means (means ± SE). Results Table 1. Performance of mean number of PLBs and fresh weight of Phalaenopsis ‘Fmk02010’ in different carbon sourced and LED lights Fresh weight (g) ± SE Mean number of PLBs ± SE Treatments Sucrose Trehalose Maltose Sucrose Trehalose Maltose L1 37.73 ± 4.40 28.13 ± 4.87 1.60 ± 0.53 0.098 ± 0.057 0.059 ± 0.033 0.043 ± 0.023 L2 26.20 ± 3.38 15.87 ± 2.89 2.73 ± 0.97 0.062 ± 0.042 0.059 ± 0.032 0.033 ± 0.019 L3 21.93 ± 3.76 8.87 ± 1.55 6.00 ± 1.35 0.066 ± 0.045 0.041 ± 0.022 0.033 ± 0.018 L4 20.47 ± 3.92 27.67 ± 3.21 2.27 ± 0.76 0.043 ± 0.024 0.137 ± 0.074 0.017 ± 0.009 L5 24.80 ± 4.49 22.80 ± 2.92 3.60 ± 0.67 0.071 ± 0.039 0.079 ± 0.042 0.028 ± 0.017 L6 11.07 ± 3.97 19.33 ± 3.44 4.47 ± 1.04 0.029 ± 0.022 0.063 ± 0.034 0.043 ± 0.023 L7 11.60 ± 3.11 15.33 ± 5.55 1.47 ± 0.45 0.026 ± 0.020 0.091 ± 0.065 0.024 ± 0.013 L8 54.13 ± 8.85 12.13 ± 2.82 14.47 ± 3.39 0.109 ± 0.063 0.027 ± 0.020 0.066 ± 0.041 L9 19.93 ± 4.83 22.73 ± 3.67 2.27 ± 1.03 0.061 ± 0.035 0.117 ± 0.062 0.020 ± 0.011 L10 26.40 ± 3.60 8.73 ± 2.19 4.80 ± 1.11 0.080 ± 00.047 0.047 ± 0.026 0.051 ± 0.027 L11 10.67 ± 4.11 36.33 ± 5.08 3.80 ± 1.76 0.020 ± 0.020 0.129 ± 0.071 0.045 ± 0.024 L12 5.47 ± 1.98 5.00 ± 1.70 8.40 ± 1.85 0.015 ± 0.015 0.066 ± 0.048 0.079 ± 0.047 L13 13.53 ± 2.88 35.13 ± 4.36 13.73 ± 2.09 0.034 ± 0.024 0.167 ± 0.098 0.112 ± 0.068 L14 19.07 ± 2.60 29.40 ± 4.45 1.47 ± 0.35 0.030 ± 0.021 0.090 ± 0.048 0.022 ± 0.013 L15 12.20 ± 2.79 32.00 ± 7.77 4.73 ± 1.27 0.056 ± 0.038 0.088 ± 0.063 0.030 ± 0.017 Table 2. Shoot growth of the Phalaenopsis during PLBs organogenesis at three different carbon sources used in culture media and cultured under different LED lights Number of shoot Shoot length Treatments Sucrose Trehalose Maltose Sucrose Trehalose Maltose L1 0 0 0 0 0 0 L2 0 0 0 0 0.38 ± 0.14 0.26 ± 0.10 L3 0 0 0 0 0.38 ± 0.14 0.04 ± 0.02 L4 0 0 0 0 0.75 ± 0.29 0.11 ± 0.05 0 0 L5 0.13 ± 0.07 0.25 ± 0.09 0.05 ± 0.05 0.08 ± 0.03 L6 0 0 0.13 ± 0.07 0.13 ± 0.13 0.08 ± 0.04 0.03 ± 0.03 L7 0 0 0 0 0.25 ± 0.13 0.05 ± 0.03 L8 0 0 0 0 0.13 ± 0.07 0.03 ± 0.01 L9 0 0 0 0 0.13 ± 0.07 0.05 ± 0.03 L10 0 0 0 0 0 0 L11 0 0 0 0 0.38 ± 0.14 0.12 ± 0.04 L12 0 0 0 0 0 0 L13 0 0 0 0 0 0 0 0 L14 0 0 0.25 ± 0.13 0.03 ± 0.03 L15 0 0 0 0 0 0 Here; L1 Control (White fluorescent light), L2 (Red LED), L3 (Green LED), L4 (Blue LED), L5 (White LED), L6 (Red + Green LED), L7 (Red + Blue LED), L8 (Red + White LED), L9 (Green + Blue LED), L10 (Green + White LED), L11 (Blue + White LED), L12 (Red + Green + Blue LED), L13 (Red + Blue + White LED), L14 (Red + Green + White LED) and L15 (Green + Blue + White LED) Here; L1 Control (White fluorescent light), L2 (Red LED), L3 (Green LED), L4 (Blue LED), L5 (White LED), L6 (Red + Green LED), L7 (Red + Blue LED), L8 (Red + White LED), L9 (Green + Blue LED), L10 (Green + White LED), L11 (Blue + White LED), L12 (Red + Green + Blue LED), L13 (Red + Blue + White LED), L14 (Red + Green + White LED) and L15 (Green + Blue + White LED) Shoot (a) Root PLBs (b) (c) Figure 1: PLBs organogenesis of Phalaenopsis (a) PLBs, shoot and root, (b) sucrose and red + white LED and (c) trehalose and blue + white LED Conclusion From the result and discussion of the current experiment it can be concluded that sucrose can be used as the carbon sources in culture media and cultured under red + white LED i.e., 1st 30 days under red LED and then 30 days under white LED for the maximum number of PLBs. We also suggested from the findings of the current study that using trehalose as carbon sources and cultured under blue + white LED. Though the combination of trehalose with blue + white LED produced less number of PLBs than the combination of sucrose with red + white LED but the PLBs from trehalose with blue + white LED was healthier than the PLBs from sucrose with red + white LED. ACKNOWLEDGEMENTS Authors are highly grateful to The United Graduate School of Agricultural Science, Ehime University, Ehime, Kochi University, Kochi and Ministry of Education, Culture, Sports, Science & Technology in Japan. III International Symposium on Horticulture in Europe - SHE2016 CIHEAM MAI, CHANIA Date: October 17-21 , 2016 Chania, Greece organized by ➢ The Mediterranean Agronomic Institute of Chania (MAICh) ➢ The Cyprus University of Technology, Department of Agricultural Sciences, Biotechnology & Food Science under the aegis of the International Society of Horticultural Sciences (ISHS) III International Symposium on Horticulture in Europe SHE2016 Programme and Book of Abstracts Chania, Greece 17 - 21 October, 2016 Conveners Dr. Panagiotis Kalaitzis, Dr. George Manganaris Chania 2016 The organization of the meeting is supported by: Conference Centre Bureau of Mediterranean Agronomic Institute of Chania-MAICh PO Box 85, 73 100 Chania, Crete, Greece T: +30 28210 35080 F: +30 28210 35001 E: confer@maich.gr Committees Organizing Committee Panagiotis Kalaitzis, Convener, Mediterranean Agronomic Institute of Chania, Greece George Manganaris, Co-convener, Cyprus University of Technology Guglielmo Costa, University of Bologna, Italy Carlos Crisosto, University of California (Davis), USA Florin Stanica, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania Athanasios Molassiotis, Aristotle University of Thessloniki, Greece Leon Terry, University of Cranfield, United Kingdom Vassillis Fotopoulos, Cyprus University of Technology Nikolaos Tzortzakis, Cyprus University of Technology International Scientific Committee Prof. Dr Franciszek Adamicki, Research Institute of Horticulture, Poland Prof Domingos Almeida, Universidad de Lisboa, Portugal Prof. Beatrix Alsanius, Swedish Univ. Agricultural Sciences, Sweden Dr Luciana Baldoni, CNR - CNR-IBBR, Perugia, Italy Dr George Baourakis, CIHEAM - MAICh, Greece Alessandro Botton, University of Padova, Italy Kjell Brännäs, Ministry of Agriculture and Forestry, Finland Dr Mathilde Causse, INRA - GAFL, Avignon, France Dr. Ramon Dolcet-Sanjuan, IRTA, Spain Prof. Dr. Károly Hrotkó, Corvinus University of Budapest, Hungary Prof. Paolo Inglese, Universit{ degli Studi di Palermo, Italy Prof. Constantinos Kittas, University of Thessaly, Greece Maria Teresa Lafuente, Consejo superior de )nvestigaciones Científicas CS)C , Valencia, Spain Dr Francois Laurens, Centre INRA Angers-Nantes, France Prof Ales Lebeda, Palacky University, Czech Republic Dr Amnon Lers, The Volcani Center, Israel Prof. Dr. Ir. Leo F.M. Marcelis, Wageningen University, The Netherlands Dr Cathie Martin, John Innes Centre, UK Dr Jean-Claude Mauget, Agrocampus-Ouest, France Dr Silvana Nicola, University of Torino, Italy Prof. Dr Dragan Nikolić, University of Belgrade, Serbia Prof. Angelos Patakas, University of Patras, Greece Dr Georgios Psarras, ELGO - Dimitra, Greece Prof. Dimitrios Savvas, Agricultural University of Athens, Greece Dr Jaime Prohens Tomas, Univ. Politècnica de València, Spain Prof. Nikolaos Tzortzakis, Cyprus University of Technology, Cyprus Prof. Dr. Dr. Christian Ulrichs, Humboldt-Universität zu Berlin, Germany Prof. Dr. Jens Norbert Wünsche, University of Hohenheim, Germany Local Organizing Committee Aristidis Stamatakis, Soil Science and Plant Diagnostics Laboratory, (MAICh) Zeina El Zein, Horticultural Genetics and Biotechnology Laboratory, (MAICh) Katerina Karapataki, Conference Center, (MAICh) Argyro Zervou, Conference Center, (MAICh) Victor Tzougrakis, Information Systems, (MAICh) Nikos Psyllakis, Design & Graphics, (MAICh) Sponsors Media Sponsors Map of the Conference Site Programme at a Glance 7 Programme Overview 18:00 – 21:00 19:30-22:30 7:30-8:15 8:15-9:00 9.00-10.00 9:00–9:30 9:30-10:00 10:00-10:30 10:30– 11:00 11:00-12:30 12:30-13:00 13:00-14:30 14.30 - 15.30 14:30-15:00 15:00-15:30 15:30-16:00 16:00-17:00 MONDAY 17 October 2016 Registration Welcome Reception at the Minoa Palace Hotel (Pool Bar Tholos) TUESDAY 18 October 2016 Registration Welcome Addresses Plenary Session Topic 1: Human Health, Nutritional Value and Horticulture - IMPERIAL HALL What can plant science do for human health The One-health concept and organic production of vegetables & fruits Parallel Session Topic 1 - Human Health, Parallel Session Topic 2 - Quality, Authentication, Nutritional Value and Horticulture - IMPERIAL Traceability & Supply Chains - IMPERIAL 4 HALL T2-O1: Phytochemical contents and overall quality of leafy T1-O1: Metabolic characterization of the anthocyaninlettuce varieties (Lactuca sativa L.) grown in a closed synthesizing tomato genotype 'Sun BlackTM' hydroponic system T1-O2: Innovative Tools to Enhance Plum Bioactive T2-O2: Fruit quality and yield influenced by site-specific Compounds and Antioxidant Activity during mechanical flower thinning in 'Gala' and 'Elstar' orchards Postharvest Storage Coffee Break Parallel Session Topic 1 - Human Health, Parallel Session Topic 2 - Quality, Authentication, Nutritional Value and Horticulture - IMPERIAL Traceability & Supply Chains - IMPERIAL 4 HALL T1-O3: Quality differences between organic and T2-O3: Impact of light quality on anthocyanin synthesis in conventional field grown vegetables - experiences from white asparagus spears (Asparagus officinalis L.) a Swedish long term field trial T1-O4: Changes in carotenoid metabolism in response T2-O4: Low residue apple production: higher production to biotic and abiotic stress in carrot risk and lower profit T1-O5: Anthocyanin elicitation for bio-sustainable T2-O5: Choosing the opposite ' Consumer attitudes and their colourant production in carrot choice of a horticultural product T1-O6: Mulch films biodegradation rate in different T2-O6: Management of sanitary and phytosanitary risks in type of soils the apple industry: the Sustain'Apple project T1-O7: Examination of ion accumulation and some T2-O7: The effect of leonardite, vermicompost, farmyard physiological characteristics of grafted eggplants grown manure on yield and leaf quality of garden cress (Lepidium under salinity conditions sativum L.) T1-O8: Savings of plant protection products by a high T2-O8: Reduction of Pre-harvest Fruit Drops in Washington level of precision in orchards Navel (Citrus sinensis Osb.) ISHS and young minds - IMPERIAL HALL Jens Norbert Wünsche, ISHS Board Member-Innovation, Outreach, Strategy Lunch Plenary Session Topic 2: Quality, Authentication, Traceability & Supply Chains - IMPERIAL HALL The regulatory networks underlying developmental shifts in fleshy fruit: from fruit setting to fruit ripening Temperate fruit crops at new era: from preharvest to postharvest Parallel Session Topic 1 - Human Health, Parallel Session Topic 2 - Quality, Authentication, Nutritional Value and Horticulture - IMPERIAL Traceability & Supply Chains - IMPERIAL 4 HALL T2-O9: Physiological, Biochemical and Histological T1-O9: Strawberry production and nutritional value of Response to High Temperature Stress At Different Stages of fruits in changing environmental conditions Development in Okra Abelmoschus Esculentus L. (Moench) T1-10: Impact of nitrogen form and supply level on T2-10: Understanding postharvest stresses on ripening growth, yield and nutritional value in hydroponicallysynchronization of Hass avocados grown Cichorium spinosum L Poster Session / Coffee Break - IMPERIAL 1,2,3 Parallel Session Topic 1 - Human Health, Nutritional Value and Horticulture - IMPERIAL HALL T1-11: Τhe role of a prolyl 4 hydroxylases in tomato fruit development 17:00-18:30 T1-12: Characterization of the polyphenolic pattern in Prunus domestica varieties with different fruit skin color by analyzing their fruit juices T1-13: Determination of nitrogen concentration in hydroponically grown spearmint T1-14: The Use of Legumes for Sustainable Agricultural Production: EUROLEGUME T1-15: Integrated pest management of the whitefly Aleyrodes proletella in vegetable brassica T1-16: Effects of some dormancy breaking agents on hormone concentration during dormancy period in flower buds of Lapins sweet cherry cultivar 8 Parallel Session Topic 2 - Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 T2-11: The Properties of Rice Hull in Soilless Agriculture Technique and The Effect of Plant Development Parameters in Lettuce (Lactuca sativa var. crispa) Cultivation T2-12: Water and cell wall contributions to apple texture T2-13: Tools for strategic management of postharvest technology: an application to the Portuguese pear industry T2-14: Pre-harvest red light emitting diode treatment effects on nitrate reduction in green vegetables T2-15: Effects of Active Modified Atmosphere Packaging with Argon and Nitrogen on Postharvest Quality of Loquat T2-16: Sanitary management practices in the French fruit production III International Symposium on Horticulture in Europe – SHE 2016 8:30 - 9:30 8:30-9:00 9:00–9:30 9:30-10:00 10:00– 10:30 10:30-11:45 WEDNESDAY 19 October 2016 Plenary Session Topic 3: Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL HALL Sustainable vegetables by full control of plant production in greenhouse horticulture Bridging the gap between genomics and fruit breeding Parallel Session Topic 3 - Genetic Resources, Parallel Session Topic 4 - Tools, Technologies, Systems Approaches & Biodiversity - IMPERIAL Innovations and Applications - IMPERIAL 4 HALL T3-O1: Genome wide association studies and whole transcriptomic survey decipher the fruit texture T4-O1: Implementation of photovoltaics on greenhouses and regulation in apple towards the selection of novel shading effect to plant growth superior accessions T3-O2: Use of Marker Assisted Selection Methods in T4-O2: Long-Term Prediction of Biosignals from Tomato Breeding in Belarus Greenhouse-grown Tomato Coffee Break Parallel Session Topic 3 - Genetic Resources, Parallel Session Topic 4 - Tools, Technologies, Systems Approaches & Biodiversity - IMPERIAL Innovations and Applications - IMPERIAL 4 HALL T4-O3: Beyond rain protection : fruits plastic protection T3-O3: High performing phenomics of berries aroma sheet as light spectrum transmission and climatic conditions by PTR-ToF-MS influence means T3-O4: Status of research, breeding and protection of T4-O4: Development of an optimal model-based ion-specific cucurbits in relation to cucurbit downy mildew: their nutrient control of horticultural crops limits and perspectives T3-O5: Metabolic profiling of sun-dried grapes T4-O5: Process-based simulation models and plant destined for the production of `Commandaria´ dessert phenotyping wine T3-O6: Advanced mathematical algorithms to T4-O6: Phenology and Fruit Set of Promising North characterize olive varieties through morphological American Shrubs in the Genus Dirca parameters T3-O7: MSAPAnalyzer: an online tool for assessing T4-07: INAPRO - Innovative Aquaponics for Professional Methylation Sensitive Amplified Polymorphic (MSAP) Application markers by comparison to reference states 12:00-13:00 Poster Session - IMPERIAL 1,2,3 13:00-14:30 14:30 - 15:30 14:30-15:00 15:00-15:30 Lunch Break Plenary Session Topic 4: Tools, Technologies, Innovations and Applications - IMPERIAL HALL New developments in dynamic controlled atmosphere storage of pome fruit The genomic approach to dissect GXE in grapevine Parallel Session Topic 3 - Genetic Resources, Parallel Session Topic 4 - Tools, Technologies, Systems Approaches & Biodiversity - IMPERIAL Innovations and Applications - IMPERIAL 4 HALL T4-O8: Optimization of cultural practices in a multiT3-O8: Sussebtiliby of SEO/Harlayne type resistant objective framework via a simple mechanistic model for cultivars to PPV-T carbon assimilation and allocation in fruit trees T3-O9: A multidisciplinary approach reveals new T4-O9: Historical Phenological Responses in Apple to aspects of apple superficial scald etiology and cold Contrasting Warming Contexts May Clarify Future Crucial resistance mechanism in Granny Smith fruit Responses in Europe T3-10: EUROCAROTEN action to Horticultural T4-10: Modelling fruit plant-pest interactions, their control Society by cultivation practices and their link to fruit quality Coffee Break Plenary Session - IMPERIAL HALL Recent trends in greenhouse design and microclimate control Phenotyping methods for horticultural crops: opportunities and challenge Parallel Session Topic 3 - Genetic Resources, Parallel Session Topic 4 - Tools, Technologies, Systems Approaches & Biodiversity - IMPERIAL Innovations and Applications - IMPERIAL 4 HALL T3-11: Investigating the carotenoid pathway of loquat T4-11: Temperature-sum models for irrigation scheduling of fruit during on-tree developmental stages vegetable crops in open field T3-12: N2 fixing potential of Gliricidia (Gliricidia sepium) when intercropped with Cocoa (Theobroma T4-12: Towards zero discharge in hydroponic cultivation cacao L.) T4-13: Non-destructive detection of the optical properties of T3-13: Epidemiology of the Esca related fungus the wax bloom of plums during storage (at different Phaeomoniella chlamydospora temperatures) 15:30-16:15 16:15-16:45 16:45 - 17:45 16:45-17:15 17:15-17:45 17:45-18:30 9 PROGRAMME OVERVIEW 8:30 - 9:30 8:30-9:00 9:00–9:30 10:00-10:30 10:30– 11:00 11:00-12:15 12:15-12:40 12:40-14:00 14:00 - 15:00 14:00-14:30 14:30-15:00 15:00-16:00 16:00-16:30 16:30 - 17:30 16:30-17:00 17:00-17:30 17:30-18:00 20:00-24:00 THURSDAY 20 October 2016 Plenary Session Topic 5: Ornamentals, Medicinal plants, Landscape & Urban Environment - IMPERIAL HALL Regulation of senescence and abscission in ornamentals by plant hormones: Horticultural use and mode of action Genetic dissection of tomato fruit quality in the genome era: new tools for in depth QTL characterization Parallel Session Topic 5 - Ornamentals, Medicinal Parallel Session Topic 3 - Genetic Resources, Systems plants, Landscape & Urban Environment Approaches & Biodiversity - IMPERIAL 4 IMPERIAL HALL T5-O1: Aromatic and Medicinal Plants Vegetative T3-14: On-tree tocochromanol biosynthesis in `Koroneiki´ Propagation Using Reduced-Cost Nursery Facilities olive fruit: an analytical and transcriptome approach T5-O2: Survival and rooting capacity of ornamental T3-15: SSR and ISSR analysis of populational variability of cuttings as related to total nitrogen, storage condition Lychnis nivalis Kit., endemic and vulnerable Romanian and shift in metabolic N pools species Coffee Break Parallel Session Topic 5 - Ornamentals, Medicinal Parallel Session Topic 3 - Genetic Resources, Systems plants, Landscape & Urban Environment Approaches & Biodiversity - IMPERIAL 4 IMPERIAL HALL T5-O3: Impact of ethanol and acetaldehyde on cut T3-16: Different Expression of Helicase Genes in Artifically carnation (Dianthus Caryophyllus L. cv, Yellow Candy) and Naturally Induced Mango Fruitlet Abscission T5-O4: Efficient adventitious root formation in Petunia T3-17: Biotechnological approach to control PPV resistance hybrida cuttings: when signals meet resources in Plum T3-18: Genetic Basis And Environmental Factors Play Role T5-O5: Inclusion in German horticulture In Cysteine Sulphoxides Content of Garlic Cultivars T5-O6: Re-investigating substrate specificity of T3-19: Molecular background of the flavanone 4-reductase dihydroflavonol 4-reductase with respect to the B-ring activity of dihydroflavonol 4-reductases hydroxylation pattern of substrates T3-20 Ethylene receptors protect the embryo of developing T5-O7: Interdisciplinary research in urban agriculture apple fruitlets from the abscission signal: an immune-like mechanism? ISHS and scientific issues - IMPERIAL HALL Silvana Nicola, University of Turin, Italy Lunch Break Plenary Session - IMPERIAL HALL Climate change and the potential mitigation role of fruit tree orchards Application of Functional Genomics in Glandular Trichomes of Cistus creticus laid to the Exploration of Labdane-type Diterpenes Biosynthesis Parallel Session Topic 4 - Tools, Technologies, Parallel Session Topic 2 - Quality, Authentication, Innovations and Applications - IMPERIAL HALL Traceability & Supply Chains - IMPERIAL 4 T4-14: Development of sweet cherry (Prunus avium L.) T2-17: Effect of Conventional and Organik Fertilization on and apricot (Prunus armenica L.) varieties and Yield and Fruit Quality of Pomegranate (Punica granatum production systems in California and Europe L.cv. Hicaznar) T4-15: Evaluation and Comparison of A New Type of T2-18: Increased phosphorus use efficiency in basil grown Temporary Immersion System (TIS) Bioreactors for hydroponically with low phosphorus concentration and saline Myrtle (Myrtus communis L.) water T4-16: Digital picture analysis for precision screening T2-19: Configuration of baby lettuce quality grown in of sour cherry progeny for necrotic leaf tissue caused by floating system in response to cultivar, cut number and Blumeriella jaapii salinity source T4-17: DiRT-qPCR for molecular biological detection T2-20: Brand Awareness Increases Likeliness to Buy of RNA encoded viruses Branded Herb and Vegetable Transplants Coffee Break Plenary Session - IMPERIAL HALL Strategic Research and Innovation Agenda for the Fruit Sector: the view of the industry Next Generation Data analysis using Flowering Plants: Case study Aquilegia species Parallel Session Topic 5 - Ornamentals, Medicinal Parallel Session Topic 4 - Tools, Technologies, plants, Landscape & Urban Environment Innovations and Applications - IMPERIAL 4 IMPERIAL HALL T4-18: Job Satisfaction and Preferences Regarding Job T5-O8: Education needs and resources for urban Characteristics of Vocational and Master Craftsman Scholars agriculture entrepreneurship and Horticulture Students in Germany T4-19: Next generation sequencing technologies for the T5-O9: The impact of the EU VAT Directive for development of molecular markers and the analysis of flowers and plants on the ornamental supply chain genome diversity in Capsicum spp GALA DINNER & LIVE MUSIC FRIDAY 21 October 2016 Professional Tours BIOLEA Organic Olive Oil Tour Karavitakis Winery Manousakis Winery Monastery of Saint Kyriaki (Agia Kyriaki) Terra Creta 10 Programme MONDAY 17 October 2016 18:00 – 21:00 Registration 19:30-22:30 Welcome Reception at the Minoa Palace Hotel (Pool Bar Tholos) TUESDAY 18 October 2016 7:30-8:45 Registration 8:45-9:00 Welcome Addresses - IMPERIAL HALL George Baourakis, Director of MAICh Apostolos Voulgarakis, Vice Governor for Chania, Region of Crete 9:00–9:30 What can plant science do for human health - IMPERIAL HALL Cathie Martin, John Innes Centre, UK 9:30-10:00 The One-health concept and organic production of vegetables & fruits IMPERIAL HALL Beatrix Alsanius, Swedish University of Agricultural Sciences (SLU), Sweden 10:00-10:30 Human Health, Nutritional Value and Horticulture Session - IMPERIAL HALL Moderator: Hrotko Karoly, Szent )stv|n University, (ungary 10:00 - 10:15 T1-O1 Metabolic characterization of the anthocyanin-synthesizing tomato genotype 'Sun BlackTM' Federica Blando, ISPA, CNR, Italy 10:15 - 10:30 T1-O2 Innovative Tools to Enhance Plum Bioactive Compounds and Antioxidant Activity during Postharvest Storage Daniel Valero, University Miguel Hernandez, Spain 10:30– 11:00 Coffee Break 11:00-12:30 Human Health, Nutritional Value and Horticulture Session - IMPERIAL HALL Moderator: Blanke Michael, University Of Bonn, Germany 11:00-11:15 T1-O3 Quality differences between organic and conventional field grown vegetables - experiences from a Swedish long term field trial Lars Mogren, Swedish University of Agricultural Science, Sweden 11:15-11:30 T1-O4 Changes in carotenoid metabolism in response to biotic and abiotic stress in carrot Emmanuel Geoffriau, IRHS Agrocampus Ouest, France 11 PROGRAMME Anthocyanin elicitation for bio-sustainable colourant production in carrot Renate M“ller, University of Copenhagen, Denmark 11:30-11:45 T1-O5 11:45-12:00 T1-O6 12:00-12:15 T1-O7 Examination of ion accumulation and some physiological characteristics of grafted eggplants grown under salinity conditions Manar Talhouni, National Center for Agricultural Research and Extension, Jordan 12:15-12:30 T1-O8 Savings of plant protection products by a high level of precision in orchards Verena Overbeck, Julius K“hn )nstitute, Germany Mulch films biodegradation rate in different type of soils Elizabeth Duarte, University of Lisbon - Instituto Superior de Agronomia, Portugal 12:00-13:00 ISHS and young minds - IMPERIAL HALL Jens Norbert W“nsche, )S(S Board Member-Innovation, Outreach, Strategy 13:00-14:30 Lunch Break 14:30-15:00 The regulatory networks underlying developmental shifts in fleshy fruit: from fruit setting to fruit ripening - IMPERIAL HALL Mondher Bouzayen, University of Toulouse, France 15:00-15:30 Temperate fruit crops at new era: from preharvest to postharvest - IMPERIAL HALL Guiglelmo Costa, University of Bologna, Italy 15:30-16:00 Human Health, Nutritional Value and Horticulture Session - IMPERIAL HALL Moderator: Wünsche Jens, University Of Hohenheim, Germany 15:30-15:45 T1-O9 Strawberry production and nutritional value of fruits in changing environmental conditionsChristoph Carlen, Agroscope, Institute for Plant Production Sciences, Switzerland 15:45-16:00 T1-10 Impact of nitrogen form and supply level on growth, yield and nutritional value in hydroponically-grown Cichorium spinosum L Georgia Ntatsi, Agricultural University of Athens, Greece 16:00-17:00 POSTER SESSION / COFFEE BREAK - IMPERIAL 1,2,3 17:00-18:30 Human Health, Nutritional Value and Horticulture Session - IMPERIAL HALL Moderator: Wünsche Jens, University Of Hohenheim, Germany 17:00-17:15 T1-11 Τhe role of a prolyl 4 hydroxylases in tomato fruit development Andreas Perrakis, Mediterranean Agronomic Inst. Of Chania, Greece 17:15-17:30 T1-12 Characterization of the polyphenolic pattern in Prunus domestica varieties with different fruit skin color by analyzing their fruit juices Sofia Vio Michaelis, TUM, Germany 17:30-17:45 T1-13 Determination of nitrogen concentration in hydroponically grown spearmint Nikolaos Tzortzakis, Cyprus University of Technology, Cyprus 12 III International Symposium on Horticulture in Europe – SHE 2016 17:45-18:00 T1-14 The Use of Legumes for Sustainable Agricultural Production: EUROLEGUME Dimitrios Savvas, Agricultural University of Athens, Greece 18:00-18:15 T1-15 Integrated pest management of the whitefly Aleyrodes proletella in vegetable brassica Ute Vogler, Agroscope, Switzerland 18:15-18:30 T1-16 Effects of some dormancy breaking agents on hormone concentration during dormancy period in flower buds of Lapins sweet cherry cultivar Ayzin B. K“den, University of Cukurova, Turkey TUESDAY 18 October 2016 7:30-8:15 Registration 8:45-9:00 Welcome Addresses - IMPERIAL HALL George Baourakis, Director of MAICh Apostolos Voulgarakis, Vice Governor for Chania, Region of Crete 9:00–9:30 What can plant science do for human health - IMPERIAL HALL Cathie Martin, John Innes Centre, UK 9:30-10:00 The One-health concept and organic production of vegetables & fruits IMPERIAL HALL Beatrix Alsanius, Swedish University of Agricultural Sciences (SLU), Sweden 10:00-10:30 Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 Moderator: Zinkernagel Jana, Geisenheim Univerity, Germany 10:00 - 10:15 T2-O1 Phytochemical contents and overall quality of leafy lettuce varieties (Lactuca sativa L.) grown in a closed hydroponic system Dharini Sivakumar, Tshwane University of Technology, South Africa 10:15 - 10:30 T2-O2 Fruit quality and yield influenced by site-specific mechanical flower thinning in 'Gala' and 'Elstar' orchards Michael Pflanz, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Germany 10:30– 11:00 Coffee Break 11:00-12:30 Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 Moderator: Zinkernagel Jana, Geisenheim Univerity, Germany 11:00-11:15 T2-O3 11:15-11:30 T2-O4 11:30-11:45 T2-O5 Impact of light quality on anthocyanin synthesis in white asparagus spears (Asparagus officinalis L.) Werner B. Herppich, Leibniz-)nstitut f“r Agrartechnik Potsdam-Bornim e. V., Germany Low residue apple production: higher production risk and lower profit Esther Bravin, Agroscope, Switzerland Choosing the opposite ' Consumer attitudes and their choice of a horticultural productSara Spendrup, Swedish University of Agricultural Sciences, Sweden 13 PROGRAMME 11:45-12:00 T2-O6 Management of sanitary and phytosanitary risks in the apple industry: the Sustain'Apple project Jean-Marie Codron, INRA France 12:00-12:15 T2-O7 The effect of leonardite, vermicompost, farmyard manure on yield and leaf quality of garden cress (Lepidium sativum L.) Nuray Akbudak, Uludag University, Turkey 12:15-12:30 T2-O8 Reduction of Pre-harvest Fruit Drops in Washington Navel (Citrus sinensis Osb.) Assist. Ilhami Tozlu, Akdeniz University, Turkey 12:00-13:00 ISHS and young minds - IMPERIAL HALL Jens Norbert Wünsche, ISHS Board Member-Innovation, Outreach, Strategy 13:00-14:30 Lunch Break 14:30-15:00 The regulatory networks underlying developmental shifts in fleshy fruit: from fruit setting to fruit ripening - IMPERIAL HALL Mondher Bouzayen, University of Toulouse, France 15:00-15:30 Temperate fruit crops at new era: from preharvest to postharvest - IMPERIAL HALL Guiglelmo Costa, University of Bologna, Italy 15:30-16:00 Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 Moderator: Botton Alessandro, University Of Padova, Italy 15:30-15:45 T2-O9 Physiological, Biochemical and Histological Response to High Temperature Stress At Different Stages of Development in Okra Abelmoschus Esculentus L. (Moench) Shahnoosh Hayamanesh, CAMDEN NSW, Australia 15:45-16:00 T2-10 Understanding postharvest stresses on ripening synchronization of Hass avocados Romina Pedreschi, Pontificia Universidad Católica Valparaíso, Chile 16:00-17:00 POSTER SESSION / COFFEE BREAK - IMPERIAL 1,2,3 17:00-18:30 Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 Moderator: Botton Alessandro, University Of Padova, Italy 17:00-17:15 T2-11 The Properties of Rice Hull in Soilless Agriculture Technique and The Effect of Plant Development Parameters in Lettuce (Lactuca sativa var. crispa) Cultivation Gamze Cakirer, Ankara University, Turkey 17:15-17:30 T2-12 Water and cell wall contributions to apple texture Marc Lahaye, INRA-BIA, France 17:30-17:45 T2-13 Tools for strategic management of postharvest technology: an application to the Portuguese pear industry Domingos Almeida, University of Lisbon, Portugal 17:45-18:00 T2-14 Pre-harvest red LED treatment effects on nitrate reduction in green vegetables Akvile Virsile, LRCAF Institute of Horticulture, Lithuania 14 III International Symposium on Horticulture in Europe – SHE 2016 18:00-18:15 T2-15 Effects of Active Modified Atmosphere Packaging with Argon and Nitrogen on Postharvest Quality of Loquat Assoc. Ayse Tulin OZ, Osmaniye Korkut Ata University, Turkey 18:15-18:30 T2-16 Sanitary management practices in the French fruit production Zouhair Bouhsina, INRA, France WEDNESDAY 19 October 2016 8:30-9:00 Sustainable vegetables by full control of plant production in greenhouse horticulture - IMPERIAL HALL Leo Marcellis, Wageningen University, The Netherlands 9:00–9:30 Bridging the gap between genomics and fruit breeding - IMPERIAL HALL Francois Laurens, INRA Angers, France 9:30-10:00 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL HALL Moderator: Tzortzakis Nikolaos, Cyprus University Of Technology, Cyprus 9:30-9:45 T3-O1 Genome wide association studies and whole transcriptomic survey decipher the fruit texture regulation in apple towards the selection of novel superior accessions Fabrizio Costa, Fondazione Edmund Mach, Italy 9:45-10:00 T3-O2 Use of Marker Assisted Selection Methods in Tomato Breeding in Belarus Aliaksandr Kilcheuski, Belarus 10:00– 10:30 Coffee Break 10:30-11:45 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL HALL Moderator: Savvas Dimitrios, Agricultural University Of Athens, Greece 10:30-10:45 T3-O3 High performing phenomics of berries aroma by PTR-ToF-MS Brian Farneti, Fondazione Edmund Mach, Italy 10:45-11:00 T3-O4 Status of research, breeding and protection of cucurbits in relation to cucurbit downy mildew: their limits and perspectives Ales Lebeda, Palacky University in Olomouc, Czech Republic 11:00-11:15 T3-O5 Metabolic profiling of sun-dried grapes destined for the production of `Commandaria´ dessert wine Savvas Constantinou, Cyprus University of Technology, Cyprus 11:15-11:30 T3-O6 Advanced mathematical algorithms to characterize olive varieties through morphological parameters Kostantinos Blazakis, Mediterranean Agronomic Inst. Of Chania, Greece T3-O7 MSAPAnalyzer: an online tool for assessing Methylation Sensitive Amplified Polymorphic (MSAP) markers by comparison to reference states Andreas Doulis, Hellenic Agricultural Organisation DEMETER, Greece 11:30-11:45 12:00-13:00 POSTER SESSION - IMPERIAL 1,2,3 13:00-14:30 Lunch Break 15 PROGRAMME 14:30-15:00 New developments in dynamic controlled atmosphere storage of pome fruit IMPERIAL HALLBart Nicolai, University of Leuven, Belgium 15:00-15:30 The genomic approach to dissect GXE in grapevine - IMPERIAL HALL Mario Pezzoti, University of Verona, Italy 15:30-16:15 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL HALL Moderator: Patakas Angelos, University Of Patras, Greece 15:30-15:45 T3-O8 15:45-16:00 T3-O9 16:00-16:15 T3-10 Sussebtiliby of SEO/Harlayne type resistant cultivars to PPV-T Kahraman G“rcan, Erciyes University, Turkey A multidisciplinary approach reveals new aspects of apple superficial scald etiology and cold resistance mechanism in Granny Smith fruit Nicola Busatto, University of Bologna, Italy EUROCAROTEN action to Horticultural Society Antonio Jes’s Meléndez Martínez, University of Seville, Spain 16:15– 16:45 Coffee Break 16:45-17:15 Recent trends in greenhouse design and microclimate control - IMPERIAL HALL Kostas Kittas, Univ. of Thessaly (UTH), Greece 17:15-17:45 Phenotyping methods for horticultural crops: opportunities and challenge IMPERIAL HALL Mark Mueller-Linow, Forschungszentrum Juelich, Germany 17:45-18:30 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL HALL Moderator: Polverari Annalisa, University Of Verona, Italy 17:45-18:00 T3-11 Investigating the carotenoid pathway of loquat fruit during on-tree developmental stages George Manganaris, Cyrpus University of Technology, Cyprus 18:00-18:15 T3-12 18:15-18:30 T3-13 N2 fixing potential of Gliricidia (Gliricidia sepium) when intercropped with Cocoa (Theobroma cacao L.) James Seutra Kaba, Piazza universit{, )taly Epidemiology of the Esca related fungus Phaeomoniella chlamydospora Melanie Molnar, Julius K“hn-Institut, Germany WEDNESDAY 19 October 2016 8:30-9:00 Sustainable vegetables by full control of plant production in greenhouse horticulture - IMPERIAL HALL Leo Marcellis, Wageningen University, The Netherlands 9:00–9:30 Bridging the gap between genomics and fruit breeding - IMPERIAL HALL Francois Laurens, INRA Angers, France 9:30-10:00 Tools, Technologies, Innovations and Applications - IMPERIAL 4 Moderator: Pedreschi Romina, Pontificia Universidad Católica De Valparaíso, Chile 16 III International Symposium on Horticulture in Europe – SHE 2016 9:30-9:45 T4-O1 Implementation of photovoltaics on greenhouses and shading effect to plant growth Assist. Aggeliki Kavga, Dept. of Agricultural Technology, T.E.I., Greece 9:45-10:00 T4-O2 Long-Term Prediction of Biosignals from Greenhouse-grown Tomato Luis Miranda, Humboldt-Universität zu Berlin, Germany 10:00– 10:30 Coffee Break 10:30-11:45 Tools, Technologies, Innovations and Applications - IMPERIAL 4 Moderator: Nikolic Dragan, University Of Belgrade, Faculty Of Agriiculture, Serbia 10:30-10:45 T4-O3 10:45-11:00 T4-O4 11:00-11:15 T4-O5 11:15-11:30 T4-O6 11:30-11:45 T4-O7 Beyond rain protection : fruits plastic protection sheet as light spectrum transmission and climatic conditions influence means Massimo Bizzarri, Oroplus® for Agricultural Division Development of an optimal model-based ion-specific nutrient control of horticultural cropsThorsten Rocksch, Humboldt-Universität zu Berlin, Germany Process-based simulation models and plant phenotyping Michel Génard, )NRA, France Phenology and Fruit Set of Promising North American Shrubs in the Genus Dirca William Graves, Iowa State University, United States of America INAPRO - Innovative Aquaponics for Professional Application Johanna Suhl, Humboldt-Universität zu Berlin, Germany 12:00-13:00 POSTER SESSION - IMPERIAL 1,2,3 13:00-14:30 Lunch Break 14:30-15:00 New developments in dynamic controlled atmosphere storage of pome fruit IMPERIAL HALL Bart Nicolai, University of Leuven, Belgium 15:00-15:30 The genomic approach to dissect GXE in grapevine - IMPERIAL HALL Mario Pezzoti, University of Verona, Italy 15:30-16:15 Tools, Technologies, Innovations and Applications - IMPERIAL 4 15:30-15:45 Moderator: Valero Daniel, University Miguel (ern|ndez, Spain T4-O8 Optimization of cultural practices in a multi-objective framework via a simple mechanistic model for carbon assimilation and allocation in fruit trees Daniele Bevacqua, INRA, France 15:45-16:00 T4-O9 Historical Phenological Responses in Apple to Contrasting Warming Contexts May Clarify Future Crucial Responses in Europe Jean-Michel Legave, INRA, France 16:00-16:15 T4-10 Modelling fruit plant-pest interactions, their control by cultivation practices and their link to fruit quality Françoise Lescourret, )NRA, France 16:15– 16:45 Coffee Break 17 PROGRAMME 16:45-17:15 Recent trends in greenhouse design and microclimate control - IMPERIAL HALL Kostas Kittas, Univ. of Thessaly (UTH), Greece 17:15-17:45 Phenotyping methods for horticultural crops: opportunities and challenge IMPERIAL HALL Mark Mueller-Linow, Forschungszentrum Juelich, Germany 17:45-18:30 Tools, Technologies, Innovations and Applications - IMPERIAL 4 Moderator: Halbwirth Heidi, Tu Wien, Austria 17:45-18:00 T4-11 Temperature-sum models for irrigation scheduling of vegetable crops in open field Jana Zinkernagel, Geisenheim University, Germany 18:00-18:15 T4-12 Towards zero discharge in hydroponic cultivation Jim Van Ruijven, Wageningen UR Greenhouse Horticulture, Netherlands 18:15-18:30 T4-13 Non-destructive detection of the optical properties of the wax bloom of plums Michael Blanke, University of Bonn, Germany THURSDAY 20 October 2016 8:30-9:00 Regulation of senescence and abscission in ornamentals by plant hormones: Horticultural use and mode of action - IMPERIAL HALL Shimon Meir, The Volcani Center, Israel 9:00–9:30 Genetic dissection of tomato fruit quality in the genome era: new tools for in depth QTL characterization - IMPERIAL HALL Matilde Causse, INRA, France 10:00-10:30 Ornamentals, Medicinal plants, Landscape & Urban Environment IMPERIAL HALL Moderator: Economou Athanasios, Aristotle University Of Thessaloniki, Greece 10:00-10:15 T5-O1 10:15-10:30 T5-O2 Aromatic and Medicinal Plants Vegetative Propagation Using ReducedCost Nursery Facilities Orlanda Viamonte Póvoa, Escola Superior Agr|ria de Elvas, )nstituto Politécnico Portalegre, Portugal Survival and rooting capacity of ornamental cuttings as related to total nitrogen, storage condition and shift in metabolic N pools Inga Matzner, Leibniz-Inst. of VegetablOrnamental Crops, Germany 10:30– 11:00 Coffee Break 11:00-12:15 Ornamentals, Medicinal plants, Landscape & Urban Environment IMPERIAL HALL Moderator: Nicola Silvana, University Of Turin, Italy 11:00-11:15 T5-O3 Impact of ethanol and acetaldehyde on cut carnation (Dianthus Caryophyllus L. cv, Yellow Candy) Asghar Ebrahimzadeh, University of Maragheh, Iran 18 III International Symposium on Horticulture in Europe – SHE 2016 11:15-11:30 T5-O4 Efficient adventitious root formation in Petunia hybrida cuttings: when signals meet resources Uwe Druege, Leibniz Institute of Vegetable and Ornamental Crops, Germany 11:30-11:45 T5-O5 Inclusion in German horticulture Meike Rombach, TUM, Germany 11:45-12:00 T5-O6 Re-investigating substrate specificity of dihydroflavonol 4-reductase with respect to the B-ring hydroxylation pattern of substrates Heidi Halbwirth, TU Wien, Austria 12:00-12:15 T5-O7 Interdisciplinary research in urban agriculture Giorgio Prosdocimi Gianquinto, University of Bologna, Italy 12:15-12:40 ISHS and scientific issues - IMPERIAL HALL Silvana Nicola, University of Turin, Italy 12:40-14:00 Lunch Break 14:00-14:30 Climate change and the potential mitigation role of fruit tree orchards IMPERIAL HALL Christos Xylogiannis, University of Basilicata, Italy 14:30-15:00 Application of Functional Genomics in Glandular Trichomes of Cistus creticus laid to the Exploration of Labdane-type Diterpenes Biosynthesis - IMPERIAL HALL Angelos Kanellis, Aristotle University of Thessaloniki, Greece 15:00-16:00 Tools, Technologies, Innovations and Applications - IMPERIAL HALL Moderator: Duarte Elizabeth, University Of Lisbon, Portugal 15:00-15:15 T4-14 Development of sweet cherry (Prunus avium L.) and apricot (Prunus armenica L.) varieties and production systems in California and Europe Stephen Southwick, OG Packing Co., Inc., United States of America 15:15-15:30 T4-15 Evaluation and Comparison of A New Type of Temporary Immersion System (TIS) Bioreactors for Myrtle (Myrtus communis L.) Ozhan Simsek, Cukurova University Agriculture Faculty, Turkey 15:30-15:45 T4-16 Digital picture analysis for precision screening of sour cherry progeny for necrotic leaf tissue caused by Blumeriella jaapii Martin Jensen, Aarhus University, Denmark 15:45-16:00 T4-17 DiRT-qPCR for molecular biological detection of RNA encoded viruses Johanna Stammler, Technische Universität M“nchen, Germany 16:00-16:30 Coffee Break 16:30-17:00 Strategic Research and Innovation Agenda for the Fruit Sector: the view of the industry - IMPERIAL HALL Joan Bonany, Mas Badia (IRTA), Spain 17:00-17:30 Next Generation Data analysis using Flowering Plants: Case study Aquilegia species - IMPERIAL HALL Christos Noutsos, Cold Spring Harbor Laboratory, USA 17:30-18:00 Ornamentals, Medicinal plants, Landscape & Urban Environment IMPERIAL HALL Moderator: Serek Margrethe, Leibniz University Hannover, Germany 19 PROGRAMME 20:00-24:00 T5-O8 Education needs and resources for urban agriculture entrepreneurship Emmanuel Geoffriau, Agrocampus Ouest - IRHS, France T5-O9 The impact of the EU VAT Directive for flowers and plants on the ornamental supply chain Frank Bunte, Fontys International Business School, Netherlands GALA DINNER & LIVE MUSIC THURSDAY 20 October 2016 8:30-9:00 Regulation of senescence and abscission in ornamentals by plant hormones: Horticultural use and mode of action - IMPERIAL HALL Shimon Meir, The Volcani Center, Israel 9:00–9:30 Genetic dissection of tomato fruit quality in the genome era: new tools for in depth QTL characterization - IMPERIAL 4 Matilde Causse, INRA, France 10:00-10:30 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL 4 Moderator: Cardi Teodoro, Crea, Italy 10:00-10:15 T3-14 On-tree tocochromanol biosynthesis in `Koroneiki´ olive fruit: an analytical and transcriptome approach Egli Georgiadou, Cyprus University of Technology, Cyprus 10:15-10:30 T3-15 SSR and ISSR analysis of populational variability of Lychnis nivalis Kit., endemic and vulnerable Romanian species Assist. Victoria Cristea, Babes-Bolyai University, Romania 10:30– 11:00 Coffee Break 11:00-12:15 Genetic Resources, Systems Approaches & Biodiversity - IMPERIAL 4 Moderator: Mauget Jean-Claude, Agrocampus Ouest, France 11:00-11:15 T3-16 Different Expression of Helicase Genes in Artifically and Naturally Induced Mango Fruitlet Abscission Patrick Winterhagen, University of Hohenheim, Germany 11:15-11:30 T3-17 Biotechnological approach to control PPV resistance in Plum Tatiana Sidorova, Institute of Bioorganic Chemistry, Russian Federation 11:30-11:45 T3-18 Genetic Basis And Environmental Factors Play Role In Cysteine Sulphoxides Content of Garlic Cultivars Assoc. Jaroslava Ovesna, Crop Research Institute, Czech Republic 11:45-12:00 T3-19 Molecular background of the flavanone 4-reductase activity of dihydroflavonol 4-reductases Christian Gosch, Vienna University of Technology, Austria 12:00-12:15 T3-20 Ethylene receptors protect the embryo of developing apple fruitlets from the abscission signal: an immune-like mechanism? Alessandro Botton, University of Padova, Italy 12:15-12:40 ISHS and scientific issues - IMPERIAL HALL Silvana Nicola, University of Turin, Italy 20 III International Symposium on Horticulture in Europe – SHE 2016 12:40-14:00 Lunch Break 14:00-14:30 Climate change and the potential mitigation role of fruit tree orchards IMPERIAL HALL Christos Xylogiannis, University of Basilicata, Italy 14:30-15:00 Application of Functional Genomics in Glandular Trichomes of Cistus creticus laid to the Exploration of Labdane-type Diterpenes Biosynthesis - IMPERIAL HALL Angelos Kanellis, Aristotle University of Thessaloniki, Greece 15:00-16:00 Quality, Authentication, Traceability & Supply Chains - IMPERIAL 4 Moderator: Almeida Domingos, University Of Lisbon, Portugal 15:00-15:15 T2-17 Effect of Conventional and Organik Fertilization on Yield and Fruit Quality of Pomegranate (Punica granatum L.cv. Hicaznar)Keziban Yazici, Recep Tayyip Erdogan University, Turkey 15:15-15:30 T2-18 Increased phosphorus use efficiency in basil grown hydroponically with low phosphorus concentration and saline water Daniele Massa, Council for Agricultural Research and Economics, Italy 15:30-15:45 T2-19 Configuration of baby lettuce quality grown in floating system in response to cultivar, cut number and salinity source Youssef Rouphael, University of Naples Federico II, Italy 15:45-16:00 T2-20 Brand Awareness Increases Likeliness to Buy Branded Herb and Vegetable Transplants Bridget Behe, Michigan State University, United States of America 16:00-16:30 Coffee Break 16:30-17:00 Strategic Research and Innovation Agenda for the Fruit Sector: the view of the industry - IMPERIAL HALL Joan Bonany, Mas Badia (IRTA), Spain 17:00-17:30 Next Generation Data analysis using Flowering Plants: Case study Aquilegia species - IMPERIAL HALL Christos Noutsos, Cold Spring Harbor Laboratory, USA 17:30-18:00 Tools, Technologies, Innovations and Applications - IMPERIAL 4 Moderator: Lebeda Ales, Palacky University, Czech Republic T4-18 T4-19 20:00-24:00 Job Satisfaction and Preferences Regarding Job Characteristics of Vocational and Master Craftsman Scholars and Horticulture Students in Germany Stephan Meyerding, Georg-August-Universität Göttingen, Germany Next generation sequencing technologies for the development of molecular markers and the analysis of genome diversity in Capsicum spp Teodoro Cardi, CREA-ORT, Centro di ricerca per l'orticoltura, Italy GALA DINNER & LIVE MUSIC 21 PROGRAMME FRIDAY 21 October 2016 Professional Tours BIOLEA Organic Olive Oil Tour Karavitakis Winery Manousakis Winery Monastery of Saint Kyriaki (Agia Kyriaki) Terra Creta 22 Posters List Poster topics 2 and 3 on Tuesday 18 2016 and Poster topics 1,4 and 5 on Wednesday 19, 2016. Poster Topic – 1 Human Health, Nutritional Value and Horticulture T1-P1 A superior antioxidant biomaterial from tomato peel wastes for biotechnological application: optimization approach by response surface methodology Alfredo Aires, Rosa Carvalho T1-P2 Allergenic potential of local, scab resistant, red fleshed and commercial apple cultivars Thomas Letschka, Valentina Cova, Pietro Delfino, Giulia Pagliarani, Walter Guerra, Michael Oberhuber T1-P3 Alternative use of fruit and its waste streams in pharma Proefcentrum Fruitteelt, Director Dany Bylemans, Marijke Jozefczak, Tessa De Baets, Tom Deckers T1-P4 Changes of chlorophylls, anthocyanins and antiradical activity in nettle (Urtica dioca L.) leaves during vegetation period Solvita Zeipina, Liga Lepse,Ina Alsina T1-P5 Dispersal of E. coli O157:H7 gfp+ in sprouting modules Emina Mulaosmanovic , Sebastian Farkas, Julia Lindén, Maria Sousa, Samareh Gharaie, Lars Mogren, Andrea Kosiba Held, Thabani Eddington S. Chirere, Beatrix Alsanius T1-P6 Effects of nitrogen level on purslane antioxidant activity Sofia de Jesus,Ricardo Santos, Isabel Alves-Pereira, Rui Machado,Rui Ferreira T1-P7 Elimination of Prune dwarf virus from sweet cherry cultivars by in vitro chemotherapy Frantisek Paprstein , Jiri Sedlak , Jaroslav Polak , Shesh Kumari T1-P8 First results on pomegranate processing for juice extraction Pietro Catania, Maria Alleri, Claudio De Pasquale,Mariangela Vallone T1-P9 Fruit subtropical plants in therapeutic and preventive nutrition Elena Shishkina T1-P10 Impact of partial rootzone drying and mulching on fruit quality and nutrient contents after storage of 'Polka' raspberries Kujtim Lepaja, Endrit Kullaj, Lavdim Lepaja T1-P11 Induced Production of Indol-3-ylmethyl Glucosinolates in Hairy Roots of Chinese cabbage (Brassica rapa subsp. pekinensis): Perspectives to Enhance the Content of Bioactive Compounds Alfredo Aires, Madeleine Neumann, Jutta Ludwig-M“ller, Maria Schöpe, Karl-(einz Van Pée T1-P12 Influences of Soil Ameliorative Plant Species on the Organic Edible Rose Culture Ana Cornelia Butcaru, Florin Stanica T1-P13 Investigation of antimicrobial properties of different berries Remigijus Daubaras, Laima Cesoniene, Antanas Sarkinas T1-P14 Nutritional and functional value-added of biofortified apple peels Claudia S|nchez, Ana )sabel Mingote, Maria Paula Duarte 23 Posters List T1-P15 Nutritive value of marketable head cabbage and waste outer leaves of Brassica oleracea L. var. capitata subvar. alba as affected by nitrogen fertilization Ryszard Kosson, Stanislaw Kaniszewski, Kazimierz Felczynski, Maria Grzegorzewska, Magdalena Tuszynska T1-P16 Peculiarities of biological active substances accumulation in persimmon fruits Sergey Khokhlov, Elena Dunaevskaya, Igor Kostenko T1-P17 Quality, nutraceutical content and antimicrobial properties of European cranberrybush (Viburnum opulus) fruits Remigijus Daubaras, Laima Cesoniene T1-P18 Searching for a Sulfur Dioxide Replacement for Table Grapes K. Howe, George Manganaris, A. Ferry-Abee, D. Gubler, C. H. Crisosto T1-P19 Sugar and total phenol contents of Hass avacado Ayse Tulin Oz, Mozhgan ZARIFIKHOSROSHAHI, Hatice OZCAN, Ebru Kafkas T1-P20 The Effect of Organic and Conventional Fruit Production Method on Mineral Composition and Sensory Properties of Cloudy Apple Juice Lagle Heinmaa, Ulvi Moor, Priit Poldma, Tonu Tonutare, Kersti Kahu, Ulla Kidmose, Roberto Lo Scalzo T1-P21 Traditional Greek Pies ("Pites"): Nutritional Profile Analysis And Benchmarking Eleni Gkika, Kyriaki Kalinteri, George Palisidis, Sofia Loupassaki, George Boskou T1-P22 Yield, quality and greenhouse gas emissions in organic and conventional fava bean crops Juan A. Fernandez , Virginia S|nchez-Navarro , Ra’l Βornoza , Angel Faz Poster Topic – 2 Quality, Authentication, Traceability & Supply Chains T2-P1 Acetamiprid in apples, degradation and residues Sanja Lazic, Dragana Sunjka, Slavica Vukovic, Srdjana Petrovic T2-P2 Acetic acid is a promising postharvest treatment to improve hygienic conditions, retain characteristic quality attributes and prolong marketability of sweet cherries Karin Hassenberg, Felix Schuhmann, Nadja Förster, Werner B. (erppich, Martin Geyer, Christian Ulrichs, Susanne Huyskens-Keil T2-P3 Apple crop-load control underneath hailnet Michael Zoth T2-P4 Assessment of heavy metal (cadmium) induced changes in physiological processes of different field crops Vita Alle, Anita Osvalde, Uldis Kondratovics, Mara Vikmane T2-P5 Chemical composition and quality of various garlic (Allium sativum L.) genotypes cultivated in Greece Georgia Ntatsi , Spyridon Petropoulos, Angela Fernandes, Lillian Barros, Isabel Ferreira T2-P6 Comparison of Yield and Some Fruit Qualty Caharacteristcis of Some Fresh and Frigo Plants of Strawberry Varieties Kader Erçik, Nesibe Ebru Kafkas, Murat Kaya, Κenan çelik, ozghan ΒAR)F)K(OSROS(A(), Mehmet Ali SARIDAS T2-P7 Different Pruning Systems in Plum (Prunus domestica L.) Effects on Fruit Yield and Quality Ayzin B. K“den, Ali K“den, Kadir Sarier, Burhanettin )mrak 24 T2-P8 III International Symposium on Horticulture in Europe – SHE 2016 Effect of biostimulants use in early potato production in Poland Wanda Wadas, Tomasz Dziugiel T2-P9 Effect of elicitor applications on the color and chlorophyll composition of leaf lettuce (Lactuca sativa L.) Nuray Akbudak, Sevinc Basay T2-P10 Effect of fruit maturity, fruit position and netting on the development of soft scald in Malus domestica 'Honeycrisp' during storage Ines Hanrahan , Stefan Roeder , James Mattheis T2-P11 Effect of Nitrogen and Phosphorus Fertilization on Growth Components, Yield and Tuber Quality Characteristics of Two Potato Cultivars Grown Under Organic Production System Alexios Alexopoulos, Theodore Varzakas, Stavros Karras, Antonia Koriki, Anastasios Kotsiras5, Ioannis Xynias T2-P12 Effect of nitrogen fertilization on lycopene content and quality traits of tomato (Lycopersicon esculentum L.) fruits for fresh market and processing Stanislaw Kaniszewski, Ryszard Kosson, Artur Kowalski, Maria Grzegorzewska, Justyna Szwejda-Grzybowska T2-P13 Effect of Sulfate anions on Nitrates accumulation and Total Kjeldahl Nitrogen content in Lettuce plants cultivated in peat-perlite mixture Karipidis Charalambos, Dimitra Douma, Paraskevi Yfanti, Konstantinos Zisis, George Patakioutas T2-P14 Effect of woodchips mulch and performance of eight blackcurrant cultivars Valda Laugale, Sandra Dane, Liga Lepse, Sarmite Strautina T2-P15 Evaluation of EMA package for half, eighth and slices fresh cut of `Spinoso sardo´ globe artichoke Anna Barbara Pisanu, Limbo Baghino, Davide Sanna, Efisio Antonio Scano T2-P16 Evaluation of highbush blueberry clonal progenies for fruit quality Irina Ancu, , Monica Sturzeanu , Song Young un , Min Kyung Kwon , Dong Whan Suh T2-P17 Evaluation of Legume-Cereal Seed Mixtures for Enhancement of Soil Fertility, Carbon Storage and Biodiversity in a Low-Intensity Olive Orchard Omar Abo Fakher , Georgios Doupis , Androniki Papafilippaki , Georgios Psarras , Georgios Koubouris T2-P18 Evaluation of the postharvest quality indicators of some strawberry cvs Monica Sturzeanu, Mihail Coman, Irina Ancu1 T2-P19 Fruit quality and yield of strawberries as affected by soil and foliar iodine fertilization Diemo Daum, Christian Meinecke, Christoph Budke, Patrick G. Lawson, Ketut A. Wijaya T2-P20 Growth and anthocyanin accumulation of purple carrots is impacted by harvesting date, but not by reduced nitrogen supply Lilian Schmidt, Sophia Sorg, Susanne Tittmann, Johannes Max, Jana Zinkernagel T2-P21 How climate change and lack of chilling may affect fruit growing in a future with warmer winters Heiko Kaufmann, Michael Blanke, Achim Kunz, Eike Luedeling T2-P22 Impact of high CO2 on ripening behaviour and discoloration of banana fruit (Musa x paradisiaca) Werner B. Herppich, Anja-Magret Mink, Guido Rux, Thanos Papageorgiou, Martin Geyer, Christian Ulrichs, Susanne Huyskens-Keil T2-P23 Impact of postharvest chlorine dioxide treatment on microbial decay and physiological quality of white asparagus (Asparagus officinalis L.) Karin Hassenberg, Werner B. Herppich, Susanne Huyskens-Keil 25 Posters List T2-P24 Impact of pre- and postharvest factors on storage quality for lamb's lettuce (Valerianella locusta L.) An Decombel, Bert Jacobs, Joris Van Lommel, Isabel Vandevelde, Bert Verlinden, Peter Bleyaert T2-P25 Influence of Nitrogen and Sulfur Fertilization on the Yield and Quality of Garlic Priit Põldma , Ulvi Moor , Tonu Tonutare , Marge Starast T2-P26 Influence of nitrogen form and concentration on yield and quality of pot grown basil Diemo Daum, Christian Frerichs, Robert Koch T2-P27 Influence of Seed Pre-treatments on Seed Germination and Early Seedling Developments of Jujube (Ziziphus jujuba Mill.) Sadiye Gozlekci, Nafiye Adak, Ilhami Tozlu T2-P28 Leaf gas exchange characteristics and leaf water potential of drought stressed linden (Tilia sp.) trees K|roly (rotkó, M|rk Steiner, M|té Vértesy T2-P29 Prunus Rootstock Testing for Peach Tolerance to Pseudomonas and Armillaria Diseases in South Carolina Gregory L. Reighard, W. Greg Henderson, David Ouellette T2-P30 Relationships among the rootstock and morphogenetic behaviour of apple tree in aspect for fruit quality Giedre Samuoliene, Alina Viskeliene, Ramunas Sirtautas, Darius Kviklys T2-P31 Research on the influence of carbon dioxide-rich atmosphere of short term storage of blueberry Daniela Veringa , Marian Vintila , Angela Mohora T2-P32 Residue analysis and dissipation of the fungicide Boscalid in table grapes, wine and distillates Christos Alexoudis, Dimitios Makaridis, Theodoros Doulaveris, Sotirios Papadopoulos, Zoe Adamidou, Zisis Vryzas T2-P33 Rootstocks and grapes cultivars response to an increasing soil water deficit in the northern part of Mexico Abelardo Nuñez-Barrios , Ramona Perez-Leal , Pasquale Cirigliano T2-P34 Strawberry yield and quality in intercrop with legumes Sandra Dane, Valda Laugale, Dace Sterne T2-P35 The crop of grafted peppers an alternative - concerning the yield and quality - for vegetables growers Marian Bogoescu T2-P36 The effect of nitrogen fertilization and postharvest treatment with 1-MCP and ethylene on durability of tomato fruits Maria Grzegorzewska, Stanislaw Kaniszewski, Ryszard Kosson, Ewa Badelek, Kalina Sikorska-Zimny T2-P37 The effect of two boron compounds on growth and chemical composition of two fruit rootstocks Georgios Sismanidis , Sofia Ntoumanidou , Vasileios Christodoulou, Christos Chatzissavvidis T2-P38 The effect of zeolite, chemical fertilizer and compost on yield of lettuce, nutrient composition and soil properties Victor Kavvadias, Zaxarias Ioannou, Aggeliki Mastoraki, Dimitrios Salivaras, Sideris Theocharopoulos T2-P39 The effects of different nitrogen and potassium levels on yield and quality of two early grape cultivars grown in different soilless media Serpil Tangolar, Semih Tangolar, Melike Ada, G“zin Tarim, Ayfer Alkan Torun, Ebru Ertargin 26 T2-P40 T2-P41 T2-P42 T2-P43 T2-P44 T2-P45 III International Symposium on Horticulture in Europe – SHE 2016 The Effects of Postharvest Water Submerging and 1-Methylcyclopropene Treatments on the Ripening of `Golden Delicious´ Apples from Two Harvest Dates Daniel Alexandre Neuwald, Leonie Hart The effects of preharvest 1-methylcyclopropene (Harvista) treatments on harvest maturity and shelf life quality of `Santa Maria´ pear cultivar Mustafa Sakaldas, Engin G“r, Mehmet Ali G“ndo du The Effects of Topping Applications at Different Levels on Yield and Quality of Summer Shoots for Yalova Cekirdeksizi Grape Cultivar Arda Akçal The Influence of Crown Shapes on Yield Quantity and Quality Parameters Cristina Moale The role of mulching materials on Lactuca sativa production - Brazil case study Paulo Fortes Neto, Nara L’cia Perondi Fortes, Kaio Pinheiro, Arthur Saraiva, Raquel Costa, Elizabeth Duarte Vegeto-Productive Behavior and Fruit Quality of Two Cultivars of Apricot, `Orange rubis®´ and 'Farbaly®', as Influenced by Different Biostimulants Giuseppe Lopriore , Francesco Lops , Annalisa Tarantino T2-P46 Yield of onion (Allium cepa L.) grown from direct seeding and some soil properties as affected by long-term organic and/or mineral fertilization Kazimierz Felczynski, Waldemar Kowalczyk T2-P47 Yields and Nitrogen Uptake of Cauliflower as Affected by N Rates and Intercropping with Pea and Clover Marco Tempesta , Giorgio Gianquinto , Markus Hauser , Massimo Tagliavini T2-P48 Reducing postharvest losses by film packaging of African nightshade (Solanum scabrum Mill.) ‘Improvement of storability and postharvest quality Christian Ulrichs , Susanne Huyskens-Keil , Bernhard Trierweiler , Arnold Opiyo , Elisha Gogo Poster Topic – 3 Genetic Resources, Systems Approaches & Biodiversity T3-P1 Identification of geographical origin of dry beans using the isotope ratio analysis Athanasios Lantavos, Katerina Kiriakou, Kostas Salmas, Angelos Patakas T3-P2 A comparative study among different olive cultivars (Olea europaea L.) reveals differences in expression levels of genes involved in fruit vitamin E biosynthesis Egli Georgiadou, Chrysa Sergendani, George Koubouris, George Manganaris, Panagiotis Kalaitzis, Vlasios Goulas, Vassilis Fotopoulos T3-P3 A Pistachio Selection from the University of California Breeding Program Shows Promise as a Commercial Cultivar for Earlier Harvest Maturity Craig Kallsen , Dan Parfitt T3-P4 Adaptation of Oregano (Origanum vulgare L.) to cultivation in Elvas Region, South Portugal Orlanda Viamonte Póvoa , Noémia Farinha , C|tia Claré T3-P5 An integrative approach to understand postharvest ripening heterogeneity of Hass avocados Romina Pedreschi, Claudia Fuentealba, Ignacia Hernandez, Bruno G Defilippi, Reinaldo Campos-Vargas, Susan Lurie, Sebastien Carpentier 27 Posters List T3-P6 Artichoke transplant production: Effects of nutrient solution delivery and leaf clipping Giuseppe Colla, Mariateresa Cardarelli, Walter Stefanoni,Antonio Fiorillo, Giudo Bernabei, Youssef Rouphael T3-P7 Biochemical characterization of leaves, burns and shells of Bouche de Betizac chestnut cultivar Bruna Laratta, Filomena Monica Vella, Luigi De Masi, Alessandra Morana,La Cara Francesco T3-P8 Biological and Economic Characteristics of New Peach Cultivars and Hybrids in the Steppe Crimea Tatyana Latsko, Anatoliy Smykov, Olga Fedorova T3-P9 Chilling requirement of nine peach cultivars grown in Southern Brazil region Flavio Herter, Chaiane Milech, Maria do Carmo Raseira T3-P10 Co-expression networks during tomato fruit ripening Panagiotis Kalaitzis, Stilianos Arhondakis, Andreas Perrakis, Craita Elena Bita, Dimitrios Kaloudas T3-P11 Construction of an almond genetic linkage map using F1 population Gulcan-2 x Lauranne by SSR markers Salih Kafkas, Aibibula Paizila, Elmira Ziya-Motalebipour, Izzet Acar, Nurgul Turemis T3-P12 Current status of registered pome fruit seedlings in Lebanon Lamis Chalak, Nagham Tabaja T3-P13 Determination The Effects of Growth Regulators on Pollen Viability and Germination Level of Some Tulip Cultivars Arda Akçal , Βeliha Gökbayrak , (akan Engin T3-P14 T3-P15 T3-P16 T3-P17 Developing new microsatellite markers in walnut Salih Kafkas, Seckin Karatas, Hayat Topcu Diversity of Citrus Germplasm in the Black Sea Region in Turkey Keziban Αazici, Mustafa Akbulut, Burcu Göksu, Nalan Bakoglu Dynamic phenotypic grapevine (Vitis vinifera L.) responses to chilling and heating stress Alberto Algarra Alarcón, Jana Barbro Winkler, Andrea Ghirardo, Stella Grando, Franco Biasioli, Georg Wohlfahrt, Jörg-Peter Schnitzler, Luca Cappellin Ectopic expression of Brassica Serine-Arginine proteins, Bra015576 and Bra018581, increases thermotolerance in Arabidopsis thaliana Soo In Lee, Eun Kyung Yoon, Jin A Kim, Mi-Jeong Jeong T3-P18 Effect of cytokinins on in vitro blastogenesis of Thymelaea tartonraira ssp. tartonraira (L.) All Maria Papafotiou, Aekaterini N. Martini T3-P19 Effect of different carbon sources on in vitro propagation of yellow passion fruit Savana Irribarem Costa , Paulo Mello-Farias , Andrio Spiller Copatti , Igor de Albuquerque T3-P20 Effect of mechanical thinning on fruitlet abscission and vegetative growth in apple Michael Zoth, Andreas Riehle, Jens N. Wuensche T3-P21 Effect on water deficit on tomato fruit quality - Genetic variability of the fruit response Nadia Bertin, Béatrice Brunel, Julien Bonnefoi, )vana Petrovic, Mathilde Causse T3-P22 Evaluation of Indigenous Fruits and Grapes Germplasm in the Republic of Srpska Gordana Djuric, Nikola Micic, Sanda Stanivukovic, Mirela Kajkut Zeljkovic, Tatjana Jovanovic Cvetkovic T3-P23 Evaluation of Onion (Allium Cepa. L) Breeding Selections for Resistance to Downy Mildew (Peronospora Destructor) In Kazakhstan Bakhytbek Amirov, Zhuldyz-Saule Amirova 28 T3-P24 III International Symposium on Horticulture in Europe – SHE 2016 Evaluation of some peach cultivars in the genotype collection of Research Institute of Horticulture in Skierniewice, Poland Miroslaw Sitarek T3-P25 External fruit quality and leaf disease susceptibility of Fragaria hybrids from intraand interspecific crosses Agnieszka Masny, Edward Zurawicz T3-P26 Factors influencing micropropagation of gooseberry (Ribes grossularia L) Danuta Kucharska,Teresa Orlikowska T3-P27 Flowering and Yield Potential of Promising Peach Hybrids from Crossing Combination Flaminia x Hale Tardiva Spadoni Dragan Nikolic, Vera Rakonjac, Aleksandar Radovic T3-P28 Genetic relationships among Pistacia vera L. cultivars based on simple sequence repeat markers Elmira Ziya-Motalebipour, Salih Kafkas, Mortaza Khodaijman, Hatice Gozel, Nergiz Oban T3-P29 Hybridization in wild pear (Pyrus pyraster) from various regions in Germany and from Luxembourg with respect to Pyrus × communis? Iris Wagner , Rolf B“ttner T3-P30 Identification of pomological characteristics of some important apricot varieties grown in Turkey Mehmet Ali SARIDAS, Sevgi PAYDAS KARGI, Nesibe Ebru Kafkas T3-P31 In silico polymorphism analysis for the development of simple sequence repeat markers in pistachio Mortaza Khodaijman, Salih Kafkas, Elmira Ziya-Motalebipour, Nergiz Coban, Hatice Gozel T3-P32 In vitro propagation of citrus rootstocks and their genetic relationships Konstantinos Salis, Ioannis Papadakis, Spyridon Kintzios, Marianna Hagidimitriou T3-P33 Inheritance of ripening terms, yield and resistance to fungal diseases at the peach Anatoliy Smykov, Olga Fedorova T3-P34 Introgression of agricultural traits and resistance from Asparagus wild relatives into the garden asparagus (Asparagus officinalis L.) Reiner Kramer, Thomas Nothnagel T3-P35 Investigations on the formation of dihydrochalcones in the model plant apple (Malus sp.) Malvina Milosevic , Annette Rompel , Christian Gosch , Heidi Halbwirth , Andreas Spornberger , Karl Stich T3-P36 Lemonato' peach: A series of cultivated clones with high fruit quality George Nanos, Nikoleta Mitsopoulou, Ekaterini Grigoriadou, Nikolaos Katis T3-P37 Micropropogation of Sideritis pisidica Boiss. Et Heldr. Apud Bentham Basar Sevindik, Tolga Izgu, Mehmet Tutuncu, Ehsan Mohammad Tagipur, Pembe Curuk, Ozer Yilmaz, Gonul Kaynak, Yesim Yalcin Mendi T3-P38 Molecular Characterization of Cyst Forming Nematodes Detected on Chinese cabbage in Gangwon, Korea Hyoung-Rai Ko, Min-A Lee, Eun-Hwa Kim, Jae-Kook Lee T3-P39 NEW CULTIVARS OF HIGH QUALITY NECTARINES AT THE CRIMEA Sergey Tsiupka, Evgeny Shoferistov, Iuliia Ivashchenko T3-P40 New Dwarf Bean Varieties Obtained in Romania Eliza Teodorescu, Floarea Burnichi, Petre Constantin T3-P41 Old is better: A multiple approach to identify hypoallergenic apple genotypes Mara Vegro , Giulia Eccher , Francesca Populin , Federica Savazzini , Giulia Pagliarani , Stefano Tartarini , Gabriella Pasini , Andrea Curioni , Andrea Antico , Alessandro Botton 29 Posters List T3-P42 T3-P43 T3-P44 T3-P45 Omic approaches to shed light on apple fruit development Francesca Populin , Giulia Eccher , Mirko Moser , Sergi Munne-Bosch , Javier Miret , Annick Moing , Mickaël Maucourt , Sylvain Guyot , Agnes Gacel, Duyen Prodhomme , Elisa Asquini , Azeddine Si-Ammour, Alessandro Botton Phenological characterization and water content of 'Housui' pear trees under mild winter conditions Jones Eloy, Maurício Gonçalves Bilharva, Bruno Carra, Priscilia L’cio, Chaiane Milech, Jessica Gonsalez Cruz, Rômulo Perez, Robson Ryu Αamamoto, Ana L’cia Soares Chaves, Paulo Mello-Farias, Flavio Gilberto Herter Polymorphic Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Markers in Apricot Kahraman G“rcan , Muhammet Ali Köse, Necati etinsa Proteolytic activity, identification and expression profile of a KDEL-CysEP gene in common lilac flowers depending on the flowering conditions Agata Jedrzejuk, Aleksandra Lukaszewska, Margrethe Serek T3-P46 Regulation of flavonoid biosynthesis in artichoke: isolation and characterization of a Myb transcription factor Emanuela Blanco , Wilma Sabetta , Anita Morgese , Donatella Negro , De Lisi Antonino , Giulio Sarli , Francesco Paolocci , Gabriella Sonnante T3-P47 RNAseq analysis of grapevine and Botrytis cinerea gene expression during latent infection of berries ("Noble Rot") Arianna Lovato, Teresa Colombo, Gianbattista Tornielli, Annalisa Polverari T3-P48 Seed Germination and Seedling Survival of Carob (Ceratonia siliqua) under Natural Conditions Recep Balkic, Sadettin G“ler, (amide G“bb“k T3-P49 T3-P50 T3-P51 Seed Germination Studies on Guava (Psidium guajava) Nafiye Adak, Ilhami Tozlu, Recep Balkic, Lokman Altinkaya, Ahmet Soydal, Hamide Gubbuk Specific stress-responses of Malus under replant conditions as an indicator for the definition of resistant/tolerant apple rootstocks Sonja L“tzenkirchen, Joana (enfrey, Michaela Schmitz Structural and functional features of leaves in some Ficus carica L. cultivars in situ and in vitro Irina Mitrofanova, Valentina Brailko, Olga Mitrofanova T3-P52 Studies regarding the new peach assortments in south-eastern Romania Corina Gavat, Liana Melania Dumitru, Alexandru Oprita, Liliana Miron T3-P53 Study Concerning the Main Characteristics of Climbing Bean Seeds Belonging V.R.D.S., Romania Germplasm Collection Eliza Teodorescu T3-P54 The behaviour of local populations of Aster novae angliae L. in different growing conditions Lucia Draghia, Mirela Murariu, Elena Liliana Chelariu, Maria Brinza T3-P55 The behaviour of some apricots varieties with late blooming in south-eastern Romania Vladut Alexandru Oprita, Corina Gavat T3-P56 The Behaviour Of Some New Cherry Tree Cultivars During The First Years After Planting Ion Caplan, Gheorghe Lamureanu, Constanta Alexe T3-P57 The effect of Arabidopsis P4H3 on glycoprotein expression and the fermentation Pathway in Response to Hypoxia Panagiotis Kalaitzis, Zeina El Zein, Theodore Spano, Klepadlo Mariola 30 T3-P58 III International Symposium on Horticulture in Europe – SHE 2016 The Expression of ETR1 and CTR1 Genes in 1-MCP Treated Spray Carnation Tolga Izgu, Esra Boz, Ozhan Simsek, Murat Ozmen, Okan Ozkaya, Omur Dundar, Jaime A. Teixeira da Silva, Yesim Yalcin Mend T3-P59 The identification of walnut cultivars from Nikita Botanical Gardens by SSR-markers Sergey Khokhlov, Ilnur Balapanov, Ivan Suprun, Sergey Tokmakov T3-P60 The Influence of the rooting substrate and growth regulators IBA and NAA in the number and length of adventive roots of hardwood cuttings in blueberry cv. Bluecrop (Vaccinium corymbosum L.) Sabri Braha , Petrit Rama T3-P61 The new walnut breeding program in Turkey Αasar Akca, Mehmet S“tyemez T3-P62 T3-P63 T3-P64 T3-P65 T3-P66 T3-P67 T3-P68 T3-P69 The peach HECATE3-like gene FLESHY plays a double role during fruit development Alessandro Botton , Angela Rasori , Fiorenza Ziliotto , Annick Moing , Mickaël Maucourt , Stéphane Bernillon , Catherine Deborde , Anna Petterle , Serena Varotto , Claudio Bonghi The Resistance of Apricot Genotypes to the Attack of Certain Pathogens in Romania Cristina Moale , Leinar Septar Usage of sewage sludge in Brassica oleracea acephala cultivation Özg“r Kahraman, Fatih Akin Valuable blackberry cultivars tested in Rubus collection at the Research Institute of Horticulture in Skierniewice, Poland Miroslaw Sitarek, Justyna Wójcik-Seliga Variability on morphologic descriptors of Portuguese M. pulegium accessions Orlanda Viamonte Póvoa, Noémia Farinha, Leandra Rodrigues,Ana Maria Monteiro Water Dynamics and Phenology of 'Eva' Apple Trees: Comparison of Different Plant Portions under Mild Winter Conditions in Southern Brazil Flavio Herter, Paulo Mello-Farias, Caroline Farias Barreto, Everton Sozo de Abreu, Henrique Bisognin Gallina, )zabel Camacho Nardello, Laura Reisdörfer Sommer, Marines Batalha Moreno, Roseli Mello Farias, Robson Ryu Αamamoto, Ana L’cia Soares Chaves Water use efficiency of hybrid vs open pollinated tomato (Lycopersicon esculentum) genotypes Rabab Sanoubar, Giuseppina Pennisi, Francesco Orsini, Fiorenzo Salvatorelli, Antonio Cellini, Francesca Ventura, Francesco Spinelli, Enrico Noli, Sonia Blasioli, Ilaria Braschi, Giorgio Prosdocimi Gianquinto Effect of various culture media on in vitro propagation of grapevine varieties Giouroukiko and Serifiotiko (Vitis vinifera L.) Katerina Biniari, Styliani Kypraiou, Maritina Stavrakaki, Despoina Bouza Poster Topic - 4 Tools, Technologies, Innovations and Applications T4-P1 A demonstrative module to process chili pepper products by family farmers in Chile Diego Munoz-Concha, Ximena Quinones, Juan Pablo Hernandez, Ricardo Castro, Nelson Loyola T4-P2 A new strategy to study intra-fruit ripening progression in apple (Malus domestica L. Borkh) Francesca Populin, Giulia Eccher, Mirko Moser,Sergi Munne-Bosch, Javier Miret, Annick Moing, Mickaël Maucourt , Sylvain Guyot6, Agnes Gacel6, Duyen Prodhomme, Elisa Asquini, Azeddine Si-Ammour, Alessandro Botton 31 Posters List T4-P3 T4-P4 Agro-industry composts as growing medium in a floating system for growing babyleaf lettuces. Added-value to suppress Pythium irregulare Juan A. Fernandez, Almudena Giménez, Catalina Egea, Ana B. Santísima-Trinidad, Margarita Ros, Jose A. Pascual Beneficial role of biofertilization on yield related characteristics of two apple cultivars and soil microorganisms under orchard conditions Marijana Pesakovic , Milan Lukic , Jelena Tomic , Zaklina Karaklajic-Stajic , Rade Miletic T4-P5 Biofortification of Ocimum basilicum L. plants with selenium Martina Puccinelli, Fernando Malorgio, Rita Maggini, Irene Rosellini, Beatrice Pezzarossa T4-P6 Changes in Surface Features of Pomegranate Fruit with on-invasive sensor technology Michael Blanke , Lutz Damerow T4-P7 Characterization of blueberry aroma by novel Fast-GC coupled with PTR-ToF-MS Brian Farneti, Iuliia Khomenko, Luca Cappellin, Marcella Grisenti, Paula Poncetta, Paolo Loretti, Matteo Ajelli, Franco Biasioli, Lara Giongo T4-P8 Characterization of nitrogen and carbon pools of organic fertilizers to predict their nitrogen release Elke Meinken, Jakob Burmann, Dieter Lohr T4-P9 Commissioning and optimization of an innovative system for electrolytic water disinfection in greenhouses Janine Berberich, Ingo Schuch, Dennis Dannehl, Yuan Gao, Uwe Schmidt T4-P10 Comparison of strawberry (F.xananssa cv. Florida Fortuna) volatiles using various SPME fibers by GC/MS Techniques Nesibe Ebru Kafkas, mozghan ΒAR)F)K(OSROS(A(), saaed Sad gh Azadi, hatice özcan T4-P11 Detection of salinity stress in greenhouse tomato based on crop reflectance Nikolaos Katsoulas , Ageliki Elvanidi , Eleni Zagari , Thomas Bartzanas , Panagiotis Kalaitzis 3 , Constantinos Kittas T4-P12 Detection of volatile compounds in Actinidia L. berries by GC-MS method Remigijus Daubaras, Laima Cesoniene, Sigita Bogacioviene, Audrius Maruska, Mantas Stankevicius, Algimantas Paulauskas T4-P13 Determination of Pesticide Residues in Sweet Fennel Seed (Foeniculum vulgare Mill.) by LC-MS/MS and GC-MS Chromatographies Nilda ERSOY, Deniz HAZAR, Serpil YILMAZ, Ibrahim BAKTIR T4-P14 Dynamic vs Constant Blue:Red LED Light Ratio: Effects on Lettuce Growth and Nutritional Quality Akvile Virsile, Ausra Brazaityte, Giedre Samuoliene, Ramunas Sirtautas, Pavelas Duchovskis, Paulius Vilemas T4-P15 Early Yield Prediction in Apple Orchard Lutz Damerow, Hong Cheng, Michael Blanke T4-P16 Effect of Surface Coating with Waxes on the Dormancy of Potato Tubers Grown from True Potato Seed (TPS) Theodora Karanisa, Alexios Alexopoulos, Konstantinos Akoumianakis, Ioannis Karapanos, Harold Passam T4-P17 Effects of electric power supply for artificial illumination of greenhouse crops at volatile current supply Tundra Ramirez, Peter Kläring T4-P18 Effects of Kaolin and Shading Treatments on Fruit Development and Colouration of Pomegranate (Punica granatum L.cv. Hicaznar) Keziban Yazici , Lami Kaynak 32 T4-P19 T4-P20 III International Symposium on Horticulture in Europe – SHE 2016 Effects of Low Chill Accumulation on Dormancy Dynamics of Different Apple Trees Cultivars Igor de Albuquerque , Paulo Mello-Farias , Robson Ryu Αamamoto , Ana L’cia Soares Chaves , Flavio Gilberto Herter Effects of plant density and the number of emitters on plant growth and nitrate concentration in spinach cultivated in substrate Rui Machado, Isabel Alves-Pereira, Rui Ferreira T4-P21 Efficient vegetative propagation of various clones of mastic tree (Pistacia lentiscus var. Chia) through rooting of shoot cuttings Stefanos Kostas , Efstathios Hatziloukas , Stefanos Hatzilazarou , Athanasios Economou T4-P22 ESTIMATED CO2 SEQUESTRATION CAPACITY OF MAPLE (ACER SP.) AND LINDEN (TILIA SP.) TREES K|roly (rotkó, Mark Steiner, Magdolna S“töri-Diószegi, M|té Vértesy T4-P23 T4-P24 Evaluation of the biodegradation rate of mulch films Paulo Fortes Neto , Nara L’cia Perondi Fortes , Gustavo Souza ,Arthur Saraiva , Raquel Costa , Elizabeth Duarte Fluorescence Intensity in Apricot Leaves Under Their Dehydration Valentina Gorina, Vadim Korzin,Nataliya Mesyaz, Yurij Ivashchen T4-P25 How to evaluate damages caused by the spotted wing drosophila on national level? Esther Bravin, Felix Gremminger T4-P26 Hypochlorite application in hydroponics with all its benefits and hazards for tomato production Dennis Dannehl, Ingo Schuch, Raquel Salazar, Uwe Schmidt T4-P27 Impact of budload and irrigation managament on yield, berry quality and phenolic composition of cv. Alphonse Lavallee Semih Tangolar, Serpil Tangolar, G“zin Tarim, Melike Ada T4-P28 Impact of nitrogen form and supply level on growth, yield and nutritional value in hydroponically-grown stamnagathi (Cichorium spinosum L.) Dimitrios Savvas, Martina Chatzigianni, Baraa Alkhaled, Aristidis Stamatakis, Ioannis Livieratos T4-P29 Improving Colour Formation in Horticulture Fruit Crops Henrike Schuhknecht, Michael Blanke, Lutz Damerow T4-P30 In vitro PLBs organogenesis of Phalaenopsis to carbohydrate sources under LED lights Mehraj Hasan , Meskatul Alam , Sultana Umma Habiba , Kazuhiko Shimasaki T4-P31 Incidence of Bemisia tabaci and Trialeurodes vaporariourum in Hydroponic Tomato Crop as Affected by Rootstock Smiljana Goreta Ban, Katja Zanic, Gvozden Dumicic, Gabriela Vuletin Selak, Slavko Perica T4-P32 InducTomE: Optimizing plant performance under controlled stress conditions aiming towards increased accumulation of secondary metabolites in tomato leaves Simone Schmittgen, Tanja Groher, Mauricio Hunsche, Georg Noga T4-P33 Influence of Drip Irrigation on Peach Tree Growth in the Chernozem of Dobrogea, Romania Leinar Septar, Cristina Moale, Cristian Paltineanu T4-P34 INVITRO PROPAGATION of `JUMBO´ BLACKBERRY CULTIVAR Nesibe Ebru Kafkas, Sina Kefayati, Salih Kafkas T4-P35 Iodine biofortification of greenhouse cucumbers cultivated in a hydroponic system Diemo Daum, Mathias Becker, Benedikt Heitmann, Patrick G. Lawson 33 Posters List T4-P36 Modern training systems for sweet cherries -Slender spindle or hedgerow for protected growing? Verena Overbeck, Michael Blanke, Michaela Schmitz T4-P37 Mulching combined with regulated deficit irrigation can be successfully applied to young pear trees Lavdim Lepaja, Endrit Kullaj, Kujtim Lepaja, Naim Krasniqi, Agim Zajmi T4-P38 New approach of electrotherapy for grapevine virus elimination Ionela Catalina Guta , Elena Cocuta Buciumeanu , Lavinia Diana Tataru, Benedict Oprescu, Carmen Mihaela Topala T4-P39 Non-invasive detection of russet on pome fruit Michael Blanke , Matthias Klemm , OLga Roettger , Lutz Damerow T4-P40 Optimization of light-emitting diodes spectrum for greenhouse plant lighting based on relative quantum efficiency Urbain NIANGORAN, LAURENT CANALE, Feng TIAN, Theodore Cisse Haba, Georges Zissis T4-P41 PIClég': A 'research, development and stakeholders' all-together initiative for Integrated Production of field vegetables in France Mathilde Causse, Vincent Faloya, Benoit Jeannequin T4-P42 Power Supply of Greenhouses by Using Volatile Electricity Grid with High Proportion of Renewables Ingo Schuch, Dennis Dannehl, Uwe Schmidt T4-P43 Productivity of baby plum tomatocv Sweetelle Peter Newton, Toni Talab T4-P44 Propagation of Scabiosa hymettia (Boiss & Spruner) by stem cuttings Maria Papafotiou, Georgia Vlachou, A. Akoumianaki-Ioannidou, Konstantinos F. Bertsouklis T4-P45 Protocol for rapid in vitro shoot multiplication of ancient apple cultivars and landraces, grown in Czech Republic Jiri Sedl|k, Frantisek Paprstein, Ales Matejicek T4-P46 T4-P47 Reduced Energy Usage in Commercial Apple Storage with Improved Air Circulation Sascha Bottling, Volker Siegismund, Marc Spuhler, Jens W“nsche, Daniel Alexandre Neuwald Research on Freezing behavior of Some Cranberry Fruit Grown in Romania Marian Vintila , Daniela Veringa , Cristian Sorica T4-P48 Selective Mechanical Thinning Technology for Horticultural Fruit Crops Lutz Damerow, Michael Blanke, Claudia Seehuber T4-P49 Studies on Rooting Performances of Carob (Ceratonia siliqua) Cuttings Hamide Gubbuk, Sadettin Guler, Recep Balkic T4-P50 The control of Epicometis hirta Poda in apple orchards with azadirachtin Slavica Vukovic, Sanja Lazic, Sonja Gvozdenac, Dragana Sunjka T4-P51 The Effect Of Biostimulators Treatment Upon Seed Breeding Of Aster Novi-Belgii Mirela Cojocariu, Lucia Draghia, Elena Liliana Chelariu T4-P52 The effect of modified atmosphere packaging on postharvest performance of two loquat cultivars Margarita Hadjipieri, Kalliopi Gavriel, Georgios Sismanidis, George Manganaris T4-P53 Unravel the complexity of apple aroma by dynamic VOCs fingerprinting Brian Farneti, Iuliia Khomenko, Mario Di Guardo, Luca Cappellin, Francesco Spinelli, Franco Biasioli, Fabrizio Costa T4-P54 Use of beneficial microorganisms to improve rooting success rate in Chamaecyparis sp. cuttings and Fusarium resilience in cyclamen Oscar Stapel, Maud Tragin 34 T4-P55 T4-P56 T4-P57 III International Symposium on Horticulture in Europe – SHE 2016 Valorization of digestate in greenhouse tomato crops Αannick Fleury, Jacques Fuchs, Céline Gilli Yield and Quality of Lettuce in Response to the Plant Position in Photovoltaic Greenhouse Antonella Sirigu, Giulia Roberta Urracci, Gianluca Carboni, Francesco Chessa, Marco Maxia, Luigi Ledda Effect of indolebutyric acid (IBA) and cane positon on rooting of rootstock's cuttings Katerina Biniari, Ioannis Daskalakis, Despoina Bouza Poster Topic - 5 Ornamentals, Landscape And Urban Environment T5-P1 T5-P2 A STUDY ON CUT FLOWER POTENTIAL OF ANTALYA Deniz (azar, Nilda Ersoy, G“lden Tamer, )brahim Baktir Anti-feeding activity of Selected Botanical Extracts and their Combinations on Plutella xylostella Larvae Nambe Jababu , Tomas Kopta , Robert Pokluda T5-P3 Characterization Descriptors For Lithuanian Lophospermum Cultivars Simas Gliozeris, Alfonsas Tamosiunas T5-P4 Effects of Gibberellic Acid Applications on Induction of Parthenocarpy in Black Myrtle Fruits Halil Ibrahim Uzun , Esra Alim , Ibrahim Baktir T5-P5 Flower color and flavonoid pigments composition of the petals in ornamental peach cultivars obtained by crossing with Prunus mira Larisa Komar-Tyomnaya,Georgy Zaitsev T5-P6 How can innovation of locally grown fruit and vegetables reduce food waste in the public procurement process? Lotta Nordmark, Madeleine Uggla, Anna Peterson, Marie Olsson T5-P7 Identification of SSR in Gardenia jasminoides Ellis de novo transcriptomic analysis Georgios Tsanakas, Maria Manioudaki, Athanasios Economou, Panagiotis Kalaitzis T5-P8 Importance of Geographical Indications (GI) in Fruit Production, Case Study; ''Finike Orange'' Lokman ALTINKAYA , Ilhami Tozlu , Recep Balkic, Hamide GUBBUK, Nafiye Adak T5-P9 Morphologic variability in Portuguese Mentha cervina accessions Orlanda Viamonte Póvoa, Leandra Rodrigues, Noémia Farinha, Ana Maria Monteiro T5-P10 Multi-species near infrared calibration models for predicting nitrogen status of ornamental cuttings ' strategies for scope extension Dieter Lohr, Peter Tillmann, Siegfried Zerche, Uwe Druege, Elke Meinken T5-P11 New Peach Cultivars by Nikita Botanical Gardens with High Resistance to Leaf Curl (Taphrina deformans Tul.) Anatoliy Smykov, Yulia Ivashchenko, Olga Fedorova, Tatyana Shishova T5-P12 Observations Related the Long-Eared Owl (Asio otus) Feeding in Two Horticultural Ecosystems Cosmin Alex. Mihai, Florin Stanica T5-P13 Ornamental peach cultivars tolerant to powdery mildew and leaf curl disease Larisa Komar-Tyomnaya 35 Posters List T5-P14 Plant extract constituents from insecticidal effects of coriander, oriental valerian, cinnamon and galangal on major pests Bueyong Park, Jun-Ran Kim, In-hong Jeong T5-P15 Radio Frequency Identification System Performance in Ornamental Plant Operations Rodney Thomas Fernandez , Ryan Gawronski , Lindsay Daschner, Lynne Sage,Sam Doane , Heather Stoven , John Lea-Cox , Bruk Belayneh, Bridget Behe T5-P16 Recent Developments In The Apple World Market: The Regionalizing Effect Of The Phytosanitary Barriers? Pasquale Lubello, Federica DeMaria T5-P17 Sternbergia lutea development at different nutrient solution levels Özg“r Kahraman, Arda Akçal T5-P18 T5-P19 Symptoms of Cu, Zn, Mn and B toxicity in ornamental Vriesea cultivars Bart Vanhoutte, Johan Ceusters, Maurice De Proft The bulblet formation of Galanthus elwessi Hook. in different IBA and NAA doses Özg“r Kahraman, Arda Akçal T5-P20 The effects of fertilization on the growth and flowering of Lilium regale species MARIA BRINZA , LUCIA DRAGHIA , ELENA CHELARIU T5-P21 The Hybridization of White Flesh Nectarine with Some Species Affecting Pomological Parameters Engin G“r, Murat Eker T5-P22 T5-P23 T5-P24 The Response of Leucojum aestivum L. to Different Methods of Vegetative Propagation on Bulblet Formation and Growth Characteristics Arda Akçal, Özg“r KA(RAMAN Watercore Severity of `Fuji´ Apples from Different Positions in the Tree Canopy under Different Crop Loads Daniel Alexandre Neuwald, Corina Serban, Roy J. McCormick, Ines Hanrahan Winter-Hardy Sweet Cherry Cultivars and Forms of Nikita Botanical Garden Ekaterina Tarasova 36 Abstracts 37 BOOK OF ABSTRACTS Keynote Speakers 38 III International Symposium on Horticulture in Europe – SHE 2016 WHAT CAN PLANT SCIENCE DO FOR HUMAN HEALTH? Yang Zhanga, Eugenio Butellia, Saleh Alseekhb, Jie Luoc, Prashant Kawar, Lionel Hilla, Angelo Santinod, Alisdair Fernieb and Cathie Martina b a Metabolic Biology Department, the John Innes Centre, Norwich Research Park, Norwich, United Kingdom Max-Planck-)nstitut f“r Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am M“hlenberg , Potsdam, Germany c College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China d Institute of Sciences of Food Production CNR Unit of Lecce, via Monteroni, 73100 Lecce, Italy Over the past 30 years the developed world has seen an enormous change in diet with the proportions of carbohydrate-dense foods increasing dramatically along with consumption of large amounts of saturated fats and foods with high levels of added sugars. The advent of the Western Diet has brought with it an increase in chronic diseases, particularly those associated with the metabolic syndrome, including Type 2 diabetes, cardiovascular diseases and certain cancers. A major concern for the future is that ensuring food security by focussing on the major crops, cereals and starchy foods, will result in a loss in dietary diversity in developing countries, leading to similar problems in obesity and accompanying chronic diseases, observed in developed nations, today. Fruit and vegetables are important sources of many phytonutrients that promote health. Because these compounds are not essential for life, they have been ignored by many nutritional and biofortification programmes. However, their inclusion in the diet is essential to meeting the objectives of food and nutritional security as defined by the FAO. The first steps that are required involve the characterisation of the bioactivity of different phytonutrients of different horticultural products and their comparative assessment to inform dietary diversification programmes and public information campaigns that emphasise the benefits of horticultural products for food and health. THE ONE-HEALTH CONCEPT AND ORGANIC PRODUCTION OF VEGETABLES & FRUITS Beatrix Alsanius Swedish University of Agricultural Sciences (SLU), Sweden To meet the grand global challenges, sustainable approaches for crop production are important tools. The wise use of resources and input means is considered in the framework of organic production systems. Also consumers go increasingly organic. Although the organic production concept is an approach for more resource efficient and environmentally sound production based on few off-farm inputs and recycling, organically grown products are often perceived as safer and healthier for the consumer compared to products from conventional or integrated production. In these contexts, concentrations of bioactive compounds and level of pesticide residues are considered. However, from a hygienic point of view, animal husbandry and plant crop production share a bigger contact interface in organic farming compared to conventional or integrated production, due to a higher engagement of animal waste products and composts used for soil improvement, as plant fertilizers as well as additives to organic growing media. Furthermore, animals may also be an integral part in crop rotation or cultural management in organic horticulture. In this presentation, we view organic production in the light of the one health - concept, i.e. the interactions between animal health, environmental health and human health. THE REGULATORY NETWORKS UNDERLYING DEVELOPMENTAL SHIFTS IN FLESHY FRUIT: FROM FRUIT SETTING TO FRUIT RIPENING Mondher Bouzayen University of Toulouse, UMR INRA/ESA-INP Toulouse, Genomics and Biotechnology of Fruits, France. The fast demographic growth and global climate change rises a major threat regarding the ability of human society to provide sufficient amounts of high nutritional and sensory quality food. In the case of fruit crops, 39 BOOK OF ABSTRACTS yield is a complex trait relying on the successful completion of the pollination/fertilization process that can be dramatically impaired by environmental factors like elevated temperature or the reduction in insect pollinator population. Poor fruit setting is one of the main cause of low yield, and uncovering new means and strategies to ensure efficient fruit set under elevated temperature is becoming a major challenge. The making of a fruit comprises a series of developmental shifts involving a complex network of interacting genes and signalling pathways. It is widely accepted that interplay between hormones is instrumental to trigger the onset of ovary development into fruit, yet, the key integrating molecular players remain poorly characterized. Using the tomato as model species, we sought to decipher the molecular events underlying the flower-to-fruit transition via combined genome-wide transcriptomic profiling and reverse genetics approaches. We previously showed that downregulation of SlIAA9, a tomato member of the Aux/IAA gene family, leads to precocious fruit development prior to flower pollination and fertilization giving rise to parthenocarpy. We now reveal that SlARF8 is a central figure of the major regulome controlling tomato fruit set which involves SlIAA9 and Sly-miR167 as key actors of this control mechanism. We also unraveled the contribution of epigenetic components to the fruit initiation and established a correlation between epigenetic marks and the expression of genes involved in the fruit set. Genome-wide transcriptomic profiling combined to chromatin immuno-precipitation and protein-protein interaction studies generated extensive multidimensional expression maps of epigenetic marks and auxin signaling factors allowing to uncover new components of the fruit set process. Elucidating the mechanisms underpinning fruit set by integrating epigenetic mechanisms and auxin signaling represents an important progress in our understanding of reproductive biology. From the scientific perspective, the data provide new insight on the convergence mechanisms between auxin signaling and epigenetic regulation associated with the fruit set. Moreover, the outcome of the study provides clues for new breeding strategies aiming at improving fruit yield in harsh environmental conditions and at producing parthenocarpic fruit. TEMPERATE FRUIT CROPS AT NEW ERA: FROM PRE-HARVEST TO POSTHARVEST Guglielmo Costa Department of Agricultural Sciences, School of Agriculture and Veterinary medicine, Bologna University, Italy Temperate fruit areas are located between ° and ° of latitude and includes, in the Northern (emisphere, most of Europe, Northern Africa, most of Asia Minor, the Eastern States of the Russian Federation, the United States of America, Southern Canada, Japan, Central and Northern China, Manchuria and Mongolia, and, in the Southern Hemisphere, Southern South America, the tip of South Africa, Southern Australia, Tasmania and New Zealand. Try to imagine what the new era of temperate fruits will be is presumptuous and challenging: probably some considerations about what we should expect can be extrapolated looking at the past to observe some of the most significant changes that occurred in the fruit "industry ". In Pre-harvest, the orchard design (training system and planting density) completely changed: from a "low or very low density planting" up to "high or very high density planting orchard". The high value of the orchard suggest the realisation of tunnel with net or plastic film for protect from hail, for fruit quality enhancement, or for ripening manipulation, for disease control, etc. The Mechanisation of the main cultural operations dramatically increased (soil processing, plant protection, harvest, etc) replacing almost completely the man or animal labour. The use of internet and devices, instrument and modelling allow take decision and driving several of the main operations to be performed in the orchard: phytosanitary defence; irrigation; definition of the proper harvest time to fulfil consumers expectation and to reduce storage diseases. In Post-harvest big changes also occurred. The use of low temperature for maintaining some fruit species for some months was more and more studied and refined starting from normal and controlled atmosphere. In the future the main strategies and technologies to be used in post harvest will consider: - an implementation of the Traceability procedure: RFID (radio frequency identification) and other types, applied to pallet boxes will allow to identify and store along the supply chain (Https://en.wikipedia.org/wiki/Radiofrequency_identification). - the use of physical methods for the prevention of storage alterations (DCA, hot water treatments, electron beam, cold plasma) or of natural substances against bacteria fungi, chilling injury; 40 III International Symposium on Horticulture in Europe – SHE 2016 - the use of Smart packaging impregnated; - with ethylene absorber substances (Ripelock of Agrofresh http://www.agrofresh.com/ripelock-technology), - or pesticide or antibacterial releasing substances or with selective permeability to O2 , CO2 and C2H4 etc.) (specific for different fruits); - a continuous monitoring of the fruits in storage (DCA with fluorescence monitoring to avoid anoxia, analyzing metabolites produced from the fruits caused by biotic diseases (electronic nose) or for ripening evolution monitoring (DAFL, etc). The Temperate fruit crops at new era must be sustainable and fulfil quality and healthy fruit production requisites. Rest on our laurels, be satisfied with the results achieved is wrong. Innovations must be considered and we all must search for them. One mission of the Horticulture is "Feeding the planet" and this will be complicate to achieve since the arable land is decreasing while the population is increasing. There is the need to find new places and strategies for growing temperate fruit: soilless, more crops in one year, vertical building, flooding greenhouses, etc. as well as a careful consideration of the vantages and disadvantages offered by GMOs. SUSTAINABLE VEGETABLES BY FULL CONTROL OF PLANT PRODUCTION IN GREENHOUSE HORTICULTURE L.F.M. Marcelis Wageningen University, Horticulture and Product Physiology group, P.O. Box 16, 6700 AA Wageningen, The Netherlands. Leo.marcelis@wur.nl Greenhouse production systems enable a high level of control of abiotic and biotic conditions. For sustainable production of high quality products, it is important to optimize all the different growth conditions in an integrated way. Crop production per unit greenhouse area has doubled during the last 25 years in The Netherlands, while the energy use has been drastically reduced. For greenhouse grown tomatoes it was estimated that about 25% of the yield improvement was due to breeding and 75% due to improved growth conditions and cultivation techniques. The higher yield of modern tomato cultivar correlate with a higher leaf photosynthesis and lower light extinction coefficient of the canopy. The growth conditions for plants have been improved significantly through understanding crop physiology in combination with new technologies. In this paper some of the recent innovations in greenhouse horticulture are discussed. Greenhouse covers that scatter direct solar radiation without influencing the transmissivity improved crop production in several crops up to 10%. In tomato this increase was due to the improved vertical and horizontal light distribution, a larger photosynthetic capacity of the lower leaves and a larger leaf area index. LED lamps have now been introduced in greenhouse horticulture. Besides energy saving, they offer many opportunities to improve growth and quality of plants, as it allows modulation of spectrum and direction of light, controlling instantaneously light intensity, and decoupling lighting from heating. Controlling the climate in a greenhouse should focus more on the microclimate near the plant organs than on the average greenhouse climate. For instance the temperature of the apical meristem may deviate from .. to .. ºC from that of the air. Leaf initiation rate depends on temperature of the apical meristem independent of the temperature of the other plant organs and this can have significant impact on the plant phenotype. A large number of these innovations began with crop and greenhouse models, which are powerful tools in research. New developments in simulating plant processes can be found in functional-structural models (FSPM) and systems biology models. They can be used to design new production systems or to predict the ideal phenotype of plants. Plant monitoring based on combinations of plant sensors (e.g. chlorophyll fluorescence) and models will be used more and more to adjust the greenhouse environment to the needs of the plants. Keywords: energy saving, diffuse light, LED, photosynthesis, crop models, plant monitoring 41 BOOK OF ABSTRACTS FRUITBREEDOMICS: AN EUROPEAN PROJECT TO HELP BRIDGING THE GAP BETWEEN BASIC RESEARCH AND BREEDING IN FRUITS. François Laurens IRHS, INRA, AGROCAMPUS-Ouest, Université d Angers, SFR QUASAV, France Apple breeding is very active all over the world. Each program aims to release new cultivars that meet the main challenges facing apple industry : high productivity, regular production, high standard fruit quality, fruit storage ability, sustainability… Response to address them has been slow due to the nature of fruit tree breeding: long term, low efficiency and hence high cost. The FP7 European research project FruitBreedomics (2011-2015) has been 52 months of intense research activity by many researchers from 28 research institutes and private companies to fullfill the goal of bridging the gap between genomics and breeding in fruit trees. The approach taken to close this gap has been comprehensive. It has encompassed the generation of new tools for phenotyping, genotyping and transcriptomics; The genomics tools enable the discovery of new associations of markers and traits of commercial interest thanks to different appraoches (Pedigree Based Analysis (PBA) and Genome Wide Association studies (GWAS). They also enable to decipher new knowledge about the European apple and peach germplasm structure. FruitBreedomics also helps to develop methods (Molecular Assited Breeding, Genomic Selection, Fast Breeding) to improve the efficiency of fruit breeding programs and plant material that commercial breeders of fruit varieties can implement and use in their day to day effort to create new cultivars. In this paper we will summarize the main results of this project and the main outputs for apple breeders. NEW DEVELOPMENTS IN DYNAMIC CONTROLLED ATMOSPHERE STORAGE OF POME FRUIT Bart Nicolaï, Maarten Hertog, Pieter Verboven KU Leuven – University of Leuven, BIOSYST/MeBioS – VCBT, Heverlee, Belgium Pome fruit are commonly stored at low temperature, a decreased oxygen partial pressure and slightly increased carbon dioxide partial pressure (controlled atmosphere storage, CA). A fruit supply of high quality can then be guaranteed during the whole year. When the oxygen concentration during storage is too low, however, the fermentation pathway becomes dominant which leads to off-flavours and the development of storage disorders. For this reason, O2 and CO2 levels during CA are kept at safe and steady but suboptimal setpoints well above their critical levels. A relatively new approach for storing fruit is based on a dynamically controlled atmosphere (DCA). Instead of a constant setpoint for O 2, the lowest O2 setpoint beyond which fermentation overtakes respiration is continuously searched based on the stress response of the fruit. With DCA, the occurrence of disorders and quality losses are further minimized compared to CA. Different techniques can be used to detect a stress signal that correlates with the onset of fermentation. These include measurement of ethanol that is produced during fermentation and changes in chlorophyll fluorescence. A relatively new detection method for DCA is based on the continuous measurement of the respiration quotient (RQ). This parameter is defined as the ratio of the CO 2 production to the O2 consumption rate, and normally has a value around 1. Driving the O2 partial pressure towards 0 kPa increases the fermentation, causing the RQ to increase significantly. In this presentation we will compare the different methods for measuring low O 2 stress of pome fruit. We will discuss the technical requirements of storage facilities that are requried for DCA. We will also show how the fruit metabolism adapts to such hypoxic conditions. 42 III International Symposium on Horticulture in Europe – SHE 2016 THE GENOMIC APPROACH TO DISSECT GXE INTERACTIONS IN GRAPEVINE S. Dal Santo1, M. Sandri2, S. Zenoni1, G.B. Tornielli1, P. Zuccolotto2, M. Fasoli1, G. De Lorenzis3, L. Brancadoro3, M. Pezzotti1 1Department of biotechnology, University of Verona, Italy Economy and Management, University of Brescia, Italy 3Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, University of Milano, Italy 2Department Grapevine, the most widely-cultivated perennial fruit crop, is also considered one of the most environmentally sensitive crop. It is characterized by remarkably phenotypic plasticity (i.e., the range of phenotypes a single genotype can express as a function of its environment) which in turn is believed to effectively buffer environmental extremes especially through transcriptomic and epigenomic reprogramming. Thus, the final phenotype (P) of a given grapevine plant is the result of the interaction between its genetic composition (G) and the environment (E). We have analyzed Genotype x Environment (GxE) interactions in two grapevine varieties by characterizing their transcriptome plasticity when cultivated in different environments. Specifically, two genotypes (Sangiovese and Cabernet Sauvignon) were cultivated in three different locations in Italy (Bolgheri -littoral Tuscany-, Montalcino – Appennine Tuscany- and Romagna -foothill area-), trained in an almost identical manner, and sampled at four developmental stages over two grapevine growing seasons, 2011 and 2012, for a total of 144 samples that were analyzed by hybridization to a whole-genome microarray. We have developed a new statistical data mining tool based on data reduction approaches which allowed a dissection of the transcriptomic data into stage-specific, cultivar-related and GxE important clusters of gene expression. This deep inspection of inner relationships between the different dataset variables allowed the identification of several candidate genes that could represent putative markers of berry quality traits in grapevine GxE interactions. Moreover, the methods used to establish our model provide a framework for the analysis of transcriptome plasticity in other crops as they respond to diverse environments. RECENT TRENDS IN GREENHOUSE DESIGN AND MICROCLIMATE CONTROL C. Kittas1, N. Katsoulas1, T. Bartzanas2 1University 2Institute of Thessaly, Department of Agricultural Crop Production and Rural Environment, Volos Greece, of Research and Technology – Thessaly, Centre for Research and Technology – Hellas, Volos, Greece. A high quality production of vegetables and ornamentals under cover with less energy, water, fertilizers and pesticides call for a better design of the greenhouse and climate control systems and for a precise comprehension of the climatic determinism of (i) the elaboration of the quality but also of (ii) the interactions between the various biotic agents living in the greenhouse. In view of these approaches, we firstly present substantial technological advances based on precision agriculture approaches and used to assess and control more rational the distributed greenhouse climate and plant activity such as wireless sensors, novel movement techniques and plant stress indicators. In addition, is analysed and discussed how the substantial progresses in Computer Fluid Dynamics (CFD) modelling, can used for the 3D characterization of the flow field, heat and mass transfer aiming in better design and management of greenhouses (structures and equipment). Furthermore, since, recent studies about the crop-climate relationships induced by the new energy saving techniques indicate that the influences of high inside air confinement, as in closed or semi closed greenhouses, on inside air humidity and CO 2 content are very important, their consequences are analyzed in terms of greenhouse water consumption. Finaly, given that protected crop production quality is also a major challenge, the new progresses in the field of the climate determinism of crop quality are also assessed. 43 BOOK OF ABSTRACTS IMAGING APPROACHES IN PLANT SCIENCES - APPLICATION POTENTIAL IN HORTICULTURAL SHOOT AND ROOT PHENOTYPING Mark M“ller-Linow, Laura Junker, Anika Wiese-Klinkenberg, Heike Schneider, Uwe Rascher, Fabio Fiorani Institute of Plant Sciences (IBG-2), Forschungszentrum Juelich GmbH, Germany Imaging methods are becoming increasingly important in plant phenotyping, namely for the quantification of traits, which characterize plant architecture and the structure of plant organs. In recent years a series of methodological approaches were introduced to plant sciences most of them used in industrial processes with a high application potential in quantitative plant phenotyping as well as for breeding and plant growing tasks. To date, the applicability is still limited to a few agricultural crops, but the diversity of important structural traits in horticultural breeding gives new opportunities to fully exploit the potential of these methods. At the Institute of Plant Sciences (IBG-2 FZ Juelich, Germany), we focus on the implementation of new sensor technologies as well as on the development of low-cost platforms for quantitative phenotyping of root and shoot properties. Camera-based approaches play a key role and here we developed a multi-camera approach for the use open field cultivations to analyze leaf area and leaf angle distributions of small sugar beet cultivations (Mueller-Linow et al. Plant Methods 2015). This method has been refined and technically improved for greenhouse tomato cultivations and resulted in new prototype and software developments with the capability to estimate various shoot and fruit traits related to color and shape. Root properties of horticultural plants were addressed in two projects, one which uses MRI technologies to resolve and quantify the root structure of petunia after drought and one which analyzes root images of different grapevine cultivars grown in the GROWSCREEN-Rhizo phenotyping research facility at IBG-2. REGULATION OF SENESCENCE AND ABSCISSION IN ORNAMENTALS BY PLANT HORMONES: HORTICULTURAL USE AND MODE OF ACTION Shimon Meir, Shoshana Salim, Srivigneh Sundaresan, Bekele Abebie, Zoya Chernov, Alon Glick, and Sonia Philosoph-Hadas Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Rishon Leziyon 7528809, Israel Ornamental products such as cut flowers and potted plants have a limited display life. The consumer's demands for high quality enforce the use of the best postharvest care for the ornamental products through the distribution chain. The main reasons for ending the decorative life of these ornamentals are leaf and flower senescence and abscission, which are programed processes and regulated by plant hormones. One of the strategies to cope with these problems is by manipulating the level and activity of various plant hormones involved in these processes, mainly auxin, ethylene, cytokinin, methyl jasmonate and gibberellin. The presentation will focus on new applications of postharvest treatments with part of these plant hormones, and elucidating their mode of action in improving the ornamentals display life. The following systems will be demonstrated: 1) Transcriptome profiling of ethylene-related molecular changes occurring in the flower and leaf abscission zones in response to auxin depletion, using tomato as a model system, to study auxin-ethylene relations in abscission regulation; 2) Application of various auxins to prevent floret abscission in cut flowers of cestrum, viburnum, and wax flower, and in bracts of bougainvillea plants by improving the effect of the ethylene inhibitor, silver thiosulfate (STS); 3) Improving the application of various auxins in Cestrum cut flowers by pulsing them at a high pH, thereby facilitating their acropetal transport in the stem and increasing their efficacy to inhibit floret abscission; 4) Application of the cytokinins benzyl adenine (BA) or thidiazuron (TDZ) to delay flower and leaf senescence in various ornamentals, and demonstrating that the TDZ operates in Grevillea cut flowers via regulation of sink-source relationship by increased activity of cell wall invertase; 5) Preventing petal senescence and color fading in yellow-pigmented cut roses by application of methyl jasmonate, which stabilizes the carotenoids in the chromoplasts and prevents their degradation. 44 III International Symposium on Horticulture in Europe – SHE 2016 GENETIC DISSECTION OF TOMATO FRUIT QUALITY IN THE GENOME ERA: NEW TOOLS FOR IN DEPTH QTL CHARACTERIZATION Elise Albert, Laura Pascual, Christopher Sauvage, Janejira Duangjit, Jean-Paul Bouchet, Frédérique Bitton, Isidore Diouf, Yolande Carretero, Mathilde Causse INRA, UR1052, Centre de Recherche PACA, Allée des Chênes CS , Montfavet Cedex, France Tomato fruit quality is characterized by a large number of components influenced by the genotypes and the environment. To better understand the genetic control of these components, quantitative trait loci (QTL) have been mapped for years. Today the genome sequence availability changes the paradigm of genetic approaches. QTL have been first identified using linkage mapping populations and positional cloning identified a few QTLs. However linkage mapping is limited to the analysis of traits differing between two parental lines. Genome-wide association (GWA) has then been proposed to assess a large range of variability. In tomato, we have shown that GWA study is possible, using the admixed nature of cherry tomato genomes that reduces the impact of population structure. Nevertheless, GWA success is limited in some regions due to the low decay of linkage disequilibrium, which varies along the genome. Rare alleles are also difficult to detect with GWA studies. Multi-parent advanced generation intercross (MAGIC) populations offer an alternative to traditional linkage and GWAs by increasing the precision of QTL mapping but with equilibrated allelic frequencies. We have developed a MAGIC population by crossing eight tomato lines whose genomes were resequenced. We showed the potential of the MAGIC population when coupled with whole genome sequencing to detect candidate single nucleotide polymorphisms (SNP) underlying the QTLs. QTLs for fruit quality traits were mapped and related to the variations detected at the genome sequence and expression levels. The advantages and limitations of the three types of population, in the context of the available genome sequence and resequencing facilities, will be discussed. CLIMATE CHANGE AND THE POTENTIAL MITIGATION ROLE OF FRUIT TREE ORCHARDS Cristos Xiloyannis, Bartolomeo Dichio, Giuseppe Montanaro Universit{ degli Studi della Basilicata Dipartimento delle Culture Europee e del Mediterraneo: Architettura, Ambiente, Patrimoni Culturali (DiCEM) – via San Rocco 3, 75100 Matera, Italy Agriculture is a key socio-economic sector and a driving force of sustainable development as it is related to a series of crucial conditions of sustainability and ecosystem services delivery including conservation of natural capital. Agriculture is also pivotal to face climate changes because it contributes to greenhouse gases by sources as well as removals, through photosynthesis, followed by losses from organic matter decomposition and microbial processes, and from human management/disturbance of the agro-ecosystems. In this regard, orchards may contribute to carbon cycling being net sink or source depending on their management. This contribution will focus the impact of sustainable and conventional management on changes of soil, biomass and litter carbon pools in orchards. Sustainable practices include retention of the pruning residuals, the supply of external carbon (e.g. compost, manure), the adoption of cover crops, while at the conventional one the soil is tilled, the pruning residuals are burned and fertilization is only mineral. At a peach orchard, sustainable practices allowed carbon to be accumulated in soil and litter (40 cm depth)at a mean annual rate of approx. 140 g m-2 yr-1 while it was only approx. 7 g m-2 yr-1 in conventionally managed orchard. The carbon accumulated in above- and below-ground standing biomass during the lifetime of the orchard may reach 25 t ha-1. Carbon budget at fruit orchards as determined through standard indexes (i.e. net ecosystem exchange, NEE or production NEP) and the impact of orchard management on soil biodiversity are also presented. Accounting for the components of the carbon balance in orchards may help to identify potential issues able to strength carbon capture capability of orchards and in turn support new environmental policy release. 45 BOOK OF ABSTRACTS With the contribution of the LIFE + financial instrument of the European Union to project LIFE14 CCM/GR/000635 CLIMATREE. GM was financially supported by an RTDb contract (n. 06/2016). APPLICATION OF FUNCTIONAL GENOMICS IN GLANDULAR TRICHOMES OF CISTUS CRETICUS LAID TO THE EXPLORATION OF LABDANE-TYPE DITERPENES BIOSYNTHESIS Antigoni Papanikolaou, Dimitra Papaefthimiou, Anthoula Kanioura and Angelos K. Kanellis Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognosy, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece Cistus creticus, a plant endogenous to Crete constitutes a natural factory for producing a plethora of terpenes among them labdane type diterpenes. These compounds are secreted from the plant s glandular trichomes that cover all aerial parts and exert antibacterial, cytotoxic and anticancer properties. In the recent years our lab has functionally characterized genes coding for a number of class-II (DXDD motif) enzymes that produce diphosphate labdane type diterpenes. These genes can use GGPP as substrate and produce a number of labdane type diterpenes mainly labda-13(E)-ene-8α,15-diol diphosphate (CcCLS, Falara et al. 2010) and labda-7,13(E)-dien-15-ol diphosphate (CcdiTPS1 and CcdiTPS2, unpublished data). Recently, chemical and RNA-seq analyses employed to two subspecies (Cistus creticus subsp. creticus and Cistus creticus subsp. eriocephalus) exhibiting differential accumulation of these compounds made it possible to isolate and functionally characterize a number of class II and I diterpene synthases. Interestingly, a Cistus BAHD alcohol acetyltransferase (CcLAT1) acetylated both labda-13(E)-ene-8α,15-diol and labda-7,13(E)-dien-15-ol producing the acetylated labdanes labda-13(E)-ene-8α-ol-15-yl acetate and labda-7,13(E)-dien-15-yl acetate. These recent findings will be discussed in relation to the complex biosynthetic pathway of labdane type diterpenes in Cistus creticus. (Supported by GR-GSRT/NSRF-09/SysTerp09-23-879 and NSRF 20072013/LS9 (189]. STRATEGIC INNOVATION AND RESEARCH AGENDA FOR THE FRUIT SECTOR: THE VIEW OF THE INDUSTRY Joan Bonany IRTA, Mas Badia, 17134-La Tallada, Spain Fruit and vegetables represent a significant percentage of the total agricultural output in the UE (37 %) although it only occupies less than 3% of the agricultural land. It is a chain value well-structured and organized. Additionally, fruit and vegetables production is under regulation of Common Market Organization of the EU. in spite of this and being a sector so much dependent on research and innovation for maintaining its competitively, here it has not been a common strategy from the industry on fixing priorities for research and innovation for the sector. In 2013, EUFRIN (www.eufrin.org), an Organization representing different European research institutes working in fruit and two sectorial organizations, AREFLH (www.areflh.org) and FRESHFEL (www.freshfel.org) decided to create a task force to establish what is has been called the Strategic Research and Innovation Agenda for Fruit and Vegetables (SIRA). In 2015, the creation of EUVRIN (www.euvrin.org) allowed to enlarge the scope of priorities for the vegetable sector. Industry wide challenges and research and innovation priorities are identified. After discussion involving representatives of the different organizations these priorities are incorporated into the Strategic Innovation and Research agenda (SIRA). Equally important is the communication effort of the SIRA among relevant authorities both at national and European level. 46 III International Symposium on Horticulture in Europe – SHE 2016 The SIRA identifies both inward and outward challenges of the fruit and vegetable chain value. At the same time, it identifies and prioritizes research and innovation needs of the different parts of the value chain to address those challenges. Presentation will cover both the process of establishing the SIRA and the innovation and research needs identified. NEXT GENERATION DATA ANALYSIS USING FLOWERING PLANTS: CASE STUDY AQUILEGIA SPECIES Christos Noutsos, iPlant Collaborative team Biology became a data-driven science due to the technological advancements in the last decade. To address the challenge of analyzing big datasets produced by high-throughput applications, NSF funded iPlant Collaborative (rebranded to CyVerse), an ongoing project that brings together Biologists, Bioinformaticians and Computer Scientists. As a Bioinformatician by training, my input in the development of the iPlant infrastructure was to: 1) capture metadata from the data generated from collaborators and help with their integration to iPlant resources, 2) design methodologies and pipelines for big-data analysis on molecular markers detection and gene expression variation, and 3) provide feedback to Computer Engineers on generating a user-friendly interface targeted to biologists. With the resources to analyze the data available, the challenge now is to retrieve meaningful biological information. My talk will center on using custom cloud services to analyze 1000 individuals of 2 species of a flowering plant, Columbines, aiming to develop a methodology for SNP discovery and to study population structure. We managed for the first time to separate genetically those 2 species and we identified the marker, which is responsible for the flower differentiation. Columbines are used in Horticulture industry. 47 BOOK OF ABSTRACTS Oral Topic – 1 Human Health, Nutritional Value and Horticulture 48 III International Symposium on Horticulture in Europe – SHE 2016 T1-O1 METABOLIC CHARACTERIZATION OF THE ANTHOCYANIN-SYNTHESIZING TOMATO GENOTYPE 'SUN BLACKTM' Federica Blando1, Helge Berland2, Miriana Durante1, Carmela Gerardi1, Andrea Mazzucato3, Giovanni Mita1, Yvind M. Andersen2 1 ISPA-CNR, Via prov. le Lecce-Monteroni, 73100 Lecce, Italy 2 Department of Chemistry Uni Bergen, Allegt 41, N-5007 Bergen, Norway; 3 Uni Tuscia, Viterbo, Italy Tomato (Solanum lycopersicum L.) fruits are not usually reported to contain anthocyanins. However, several species closely related to cultivated tomato, such as S. chilense, S. cheesmaniae, S. lycopersicoides and S. habrochaites) biosynthesize anthocyanins in the subepidermal tissue, both in green and in red fruit stages, resulting in a purple skin color. Sun Black TM is a trademark protected tomato line obtained at Tuscia University (Viterbo, Italy), characterized by a remarkable phenotype with deep purple pigmentation in the epicarp, due to an increased level of anthocyanins in the peel. Such line has been obtained by the combination of the Anthocyanin fruit (Aft) allele from S. chilense (a gene increasing the anthocyanin content of the fruit) with atroviolaceum (atv) from S. cheesmaniae a gene enhancing the anthocyanin presence on stems and leaves . Sun Black is therefore a breeding product, not a GMO product. This aspect is considered an added value for the market. In fact Sun Black tomato has been available in July on the )talian market, thanks to an exclusive one-year agreement between the owner of the trademark and Unicoop Firenze. We report here the chemical characterization and structure elucidation of the unique anthocyanins found in the epiderm of Sun Black tomato, as well as other bioactive compounds catotenoids, polyphenols, vitamin C) of the whole fruit. The results show the unique features of this tomato genotype as a novel food with functional properties. T1-O2 INNOVATIVE TOOLS TO ENHANCE PLUM BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY DURING POSTHARVEST STORAGE Daniel Valero 1, Alejandra Martínez-Espl| , Pedro J Βapata , María Serrano 2, Salvador Castillo 2 1 University Miguel Hernandez, Ctra. Beniel Km. 3,2, 3312 Orihuela (Alicante), Spain 2 Ctra. Beniel km. 3,2, 03312 Alicante Orihuela, Spain Plum fruits are known to contain a wide range of bioactive compounds such as phenolics, anthocyanins, carotenoids and vitamins which contribute to the claimed health-benefits after their consumption. However, the postharvest losses are accompanied by reductions in the concentration of the phytochemicals. In the present work, methyl jasmonate MeJA or methyl salicylate MeSA were applied to Black Splendor plum and then stored for 50 days under refrigeration plus. Both treatments were effective on retarding the quality parameters related to quality such as colour, firmness and acidity. However, during storage both MeJA and MeSA treatments maintained higher concentration of total phenolics and antioxidant activity. Overall, results suggest that these treatments are useful to increase the health-promoting compounds of plum during storage. 49 BOOK OF ABSTRACTS T1-O3 QUALITY DIFFERENCES BETWEEN ORGANIC AND CONVENTIONAL FIELD GROWN VEGETABLES - EXPERIENCES FROM A SWEDISH LONG TERM FIELD TRIAL Lars Mogren 1, Per Modig 2, Anita Gunnarsson 2 1 Slu, Swedish University of Agricultural Sci, Dep. of Biosystems and technology, Po Box 103, Se-23053 Alnarp, Sweden 2 HIR Skane AB, Helgegarden, Skepparslovsvagen 258, Box 9084, SE-291 09 Kristianstad, Sweden There is a debate regarding differences in quality between conventionally and organically grown vegetables. Sometimes the arguments are ideology driven rather than fact based. It is complicated to compare trials performed with different cultivars grown under different climate and management conditions. Usually the results are hard to generalize. To get rid of some of the confounding factors, a long term crop rotation trial has been performed at Önnestad in Southern Sweden. A number of conventional and organic rotations has been grown at the same site for more than 20 years. Some of the rotations contain field grown vegetable crops. In order to evaluate the impact on produce quality samples of carrots (Daucus carota) (1 conventional, 2 organic rotations), beet root (Beta vulgaris) (1 conventional, 1 organic rotations), potato (Solanum tuberosum) (1 conventional, 2 organic rotations) and onion (Allium cepa) (1 conventional, 2 organic rotations) was collected in 2014 and 2015. Yield and dry matter content was documented at harvest. Samples were frozen and freeze dried for sugar and bioactive compounds analysis in 2016. The quality attributes will be correlated to field data from each crop rotation. By this it will hopefully be possible to both identify causes of differences and quantify these differences. T1-O4 CHANGES IN CAROTENOID METABOLISM IN RESPONSE TO BIOTIC AND ABIOTIC STRESS IN CARROT Florent Perrin 1, Cécile Dubois-Laurent 2, Sébastien (uet 2, Didier Peltier 1, Séverine Gagné 1, Emmanuel Geoffriau 2 1 )R(S Université Angers, rue G. Morel, 2 IRHS Agrocampus Ouest, 42 rue G. Morel, Beaucouzé, France Beaucouzé, France Synthesized by plants, carotenoids are key pigments as photoprotective compounds in chlorophyll organs and help attract pollinators and dispersers for non-chlorophyll organs such as flowers and fruits. However, in underground organs, such as roots or tubers, their role is not clearly determined. Carotenoids also represent an important class of human health metabolites as precursors of vitamin A. The carrot root, an important vegetable consumed worldwide, contains high concentrations of carotenoids, and therefore represents an interesting model to understand the accumulation in these pigments. If some knowledge about genetic determinism exists, a few studies have highlighted the impact of environmental factors on the accumulation of carotenoids in carrots. This work thus aims to (i) determine whether environmental factors modulate the accumulation of carotenoids in carrot roots and leaves, and (ii) identify the regulation levels of the carotenoid accumulation. In this work, two studies were conducted on a panel of genotypes with various root color. The first study was to highlight the impact of two contrasted growing periods for temperature and global radiation on the carotenoid accumulation and the expression level of biosynthetic pathway genes. The second study aimed to determine more precisely the impact of individual and combined stress (water restriction, Alternaria dauci inoculation) on the carotenoid accumulation. Unfavorable growing conditions or A. dauci infection result in reduced carotenoid content in roots, which can be partly explained by changes in gene expression. 50 III International Symposium on Horticulture in Europe – SHE 2016 T1-O5 ANTHOCYANIN ELICITATION FOR BIO-SUSTAINABLE COLOURANT PRODUCTION IN CARROT Gregorio Barba , Stephan Glied , (enrik L“tken , Bjarne Joernsgaard3, Renate M“ller 1, 1 Hojbakkegaard Alle 13, Taastrup, Denmark 3 Hojbakkegaard Alle 30, Taastrup, Denmark There is increasing demand for natural food colourants that can substitute synthetic colours due to both legislative restrictions and consumer concerns. Black or purple carrots (Daucus carota ssp. sativus var. atrorubens) have strong bluish-purple colour due to the natural pigment anthocyanin, which is used as natural food colourant due to its high pH, light, and heat stability (labelled E163 in Europe). However, new production and storage strategies of black carrot are required in order to increase pigment yield and, therefore, to be competitive against synthetic colours. )n the present study, the black carrot variety Deep Purple was used in field and postharvest experiments conducted in the 2014 and 2015 growing seasons in Denmark, to determine if various field and postharvest treatments can improve carrot anthocyanin content and yield. Total monomeric anthocyanins were measured spectrophotometrically, and anthocyanin composition was analysed by both HPLC-DAD and LCMS/Q-TOF. The results documented substantial increase in anthocyanin concentration upon some of the elicitation treatments, whereas root size was not affected. Six major cyanidin-based anthocyanin forms were detected, being the acylated (i.e. more stable) anthocyanins clearly predominant. In addition, a correlation between enhanced anthocyanin contents and decreased root dry matter and sugar contents was found. Taken together, these traits make elicited Deep Purple carrots a suitable basis for obtaining natural colourants. T1-O6 MULCH FILMS BIODEGRADATION RATE IN DIFFERENT TYPE OF SOILS Elizabeth Duarte 1, Artur Saraiva 1, Raquel Costa 1, Gustavo Souza 2, Nara Fortes 2, Paulo Fortes 2, 1 Instituto Superior de Agronomia, Tapada da Ajuda, 1349-017 Lisboa, Portugal 2 Universidade de Taubaté, Rua de março, , Centro, Taubaté, Brazil Nowadays, the use of Polyethylene (PE) mulch on horticultural crops has negative environmental impacts. To overcome these problems, biodegradable mulches were developed once they can be incorporated at the end of the crop cycle, leading to an environmental more sustainable agriculture. The aim of this study was to perform respirometric tests in standardized aerobic conditions using two methods: one in batch conditions at ºC and the other in continuous mode at ºC . Biodegradation tests were developed in three types of soils: Ferralsols (T1); Calcisols (T2) and Arenosols (T3) with: 21 g/Kg organic matter (OM), sandy-loam texture, 5.8 pH (H2O); 11 g/Kg OM, clay texture, 7.6 pH (H2O); and 8 g/Kg OM, sand texture, 6.9 pH (H2O), respectively. Agrobiofilm® µm, black was used, in Brazil and Portugal, at real scale with same family crops: Asteraceae, Solanaceae and Cucurbitaceae, for that reason it was the biodegradable mulch selected. At Taubaté University was developed, in T soil, a respirometric test in batch system to measure CO production and soil microbial biomass. At Lisbon University CO2 production was measured in continuous aeration test using the soils T1, T2 and T3. The results obtained regarding the Brazil test showed that CO2 production was higher on Agrobiofilm® modality with . against 0.69 mg CO2/gsoil on bare soil modality. The same behaviour was followed on microbial carbon values with . µg/gsoil in Agrobiofilm® and . µg/gsoil in bare soil. The data obtained from the trials performed on Portugal was for T , T and T , on Agrobiofilm modality: 3.04; 0.88; 0.74 mg CO2/gsoil, against bare soil: 1.88; 0.58; 0.50 mg CO2/gsoil, respectively. From results obtained, CO2 production on continuous aeration methodology was 2.5 times higher than batch methodology. Overall the correlation between biodegradation rate and different soils characteristic s shows a promising approach emphasising the complex mechanisms involved. 51 BOOK OF ABSTRACTS T1-O7 EXAMINATION OF ION ACCUMULATION AND SOME PHYSIOLOGICAL CHARACTERISTICS OF GRAFTED EGGPLANTS GROWN UNDER SALINITY CONDITIONS Manar Talhouni 1, Kenan Sonmez 2, Sevinc Kiran 3, Sebnem Kusvuran 4, Sebnem Ellialtioglu 5, 1 Amman - Jordan, Amman, Jordan 2 Eskisehir Osmangazi University, Eskisehir, Turkey 3 Central Research Institute ofSoil and Water, Ankara, Turkey 4 Cankiri Karatekin Universitesi, Kizilirmak, Cankiri, Turkey 5 Ankara Universitesi, Faculty of Agricultur, Ankara, Turkey Salinity is one of the most severe environmental stresses, and it affects over % of the world s irrigated land. Eggplant (Solanum melongena L.); the third most important vegetable crop worldwide after potato and tomato; is relatively salt sensitive. Grafting over tolerant rootstocks is an effective tool to avoid or reduce yield losses caused by salinity stress. In this study, different rootstock/scion eggplant combinations where grown under two salinity treatments 1.8-2 dS/m (control) and 6-7 dS/m (stress) in pots under greenhouse conditions. Four eggplant genotypes were used as rootstock Köksal F , AGR , Vista, and the Turkish breeding line Burdur), while two genotypes were used as scion (Naomi F1 and Artvin). Concerning leaf area, chlorophyll content, and leaf ions content (Na, Cl, K and Ca) parameters tested; there were significant differences between combinations. Köksal and Vista rootsocks showed the best performance under salinity conditions and were more tolerant to salinity, and were efficient in preventing Na ions to transfer to the plants upper parts. Burdur genotype showed a good performance as rootstock in comparison to the commercial genotype, and it also showed that it is a potential genetic source to be used in breeding programs for new eggplant rootstocks tolerant to salinity. T1-O8 SAVINGS OF PLANT PROTECTION PRODUCTS BY A HIGH LEVEL OF PRECISION IN ORCHARDS Verena Overbeck1, Tanja Pelzer1, Jonas Huhs2, Jens Karl Wegener1 Julius K“hn )nstitute, Messeweg -12, 38104 Braunschweig, Germany 2 Chamber of Agriculture Lower Saxony, Moorende 53, 21635 Jork, Germany For fruit growing plant protection products (PPP) are required to maintain yield and to fulfill high quality standards. In vertical crops the application of PPP is made by active air support, which can result in unwanted displacement of PPP into the environment. These amounts rise when trees are bare and gaps in the canopy are larger. In densely populated regions and those with a high density of ditches, the reduction of these amounts and drift mitigation are important sociopolitical goals. Modern sensor systems offer the possibility for precise target and gap detection and can ensure an appropriate switching of single nozzles. This innovative system allows a high saving potential of PPP and a precise application onto the target area in each tree. In the project LADUS and the follow-up project named OLSVA, a concept of an optimal combination of sensors and nozzles for gap detection was further developed. Different sprayers were equipped with a high number of new optical infrared-sensors to optimize the scanning field in the orchard. In comparison with previous sensor systems, the new sensors detect single bare branches without focusing a second tree row and neglect branches in the immediate area. In year 2014, field experiments in Jork have shown that a precise application can reduce the output quantity of PPP, such as 40% in a ten-year-old apple orchard. The efficiency was significantly influenced by software-supported switch-on and switch-off of the nozzles. The evaluation of the losses in the gap showed lower values compared to conventional full application, which could also result in a drift reduction and more efficient application onto the target area.The aim of the project is to develop marketable sprayers with different fan types as well as a retrofitting kit for sprayers in use, which fulfill the requirements for a high biological efficiency. 52 III International Symposium on Horticulture in Europe – SHE 2016 T1-O9 STRAWBERRY PRODUCTION AND NUTRITIONAL VALUE OF FRUITS IN CHANGING ENVIRONMENTAL CONDITIONS Christoph Carlen1, Pamela Crespo , Andre Ançay 1 Agroscope, Institute for Plant Production Sciences, Route des Vergers 18, 1964 Conthey, Switzerland 2 Federal Office for Agriculture FOAG, 3003 Bern, Switzerland Recent scientific evidences pointed out the importance of health promoting compounds in strawberries in relation to their high level of antioxidants including vitamin C and phenolic compounds combined with their wide consumption. The main source of variation in the composition of health related compounds in strawberries is considered to be the genotype. In addition to genotypic differences, numerous studies have shown that environmental parameters can affect the concentration of anthocyanins and antioxidant activity in strawberries. Climate change is generally recognized as the major environmental problem facing the globe. The consequences are an increased variability of temperature, rainfall and storms, as well as a higher frequency of extreme events. In such conditions cultivars with a high plasticity assure higher tolerance to climate injuries and a higher stability of yield and fruit quality. The aim of this study was to characterise the genetic effects on yield and on health related compounds in fruits and yield of June-bearing strawberry cultivars. Furthermore, the stability of these compounds in changing environments was analysed. The variations in environmental conditions were obtained by cultivating these genotypes in upland and lowland conditions, as well as with and without plastic tunnel covering. This study showed significant differences in fruit yield and in the content of health promoting compounds between the nine strawberry cultivars. Antea and Clery appeared to be promising cultivars for their high vitamin C resp. high anthocyanin content. Antioxidant capacity, total phenolic compounds and vitamin C were much more affected by the genotype than by environmental conditions. In contrast, fruit yield was strongly affected by environmental conditions. Furthermore, cultivars responded differently to environmental variations and genotype stability seems the most effective way to reduce the variation due to unpredictable year specific weather conditions. In this study the cultivar Clery showed the greatest stability over year and environment in their yield and health related compounds. In conclusion, for strawberries, the role of the genotype on health related compounds appeared to be dominant over environmental variation. However, the genotypes responded differently to changing environments. T1-10 IMPACT OF NITROGEN FORM AND SUPPLY LEVEL ON GROWTH, YIELD AND NUTRITIONAL VALUE IN HYDROPONICALLY-GROWN CICHORIUM SPINOSUM L Martina Chatzigianni1, Ioannis Livieratos2, Baraa Alkhaled2, Aristidis Stamatakis2, Georgia Ntatsi1, Dimitrios Savvas1 1 Agricultural University of Athens, Laboratory of Vegetable Crops, Iera Odos 75, 11855 Athens, Greece 2 Mediterranean Agronomic Institute of Chania, Makedonias Str. 1, 73100 Chania, Crete, Greece The objective of this study was to determine the impact of total-N concentration and the ratio of -NH4 to total-N on growth, yield, and quality of two stamnagathi (Cichorium spinosum L.) landraces (Tavri and Stavros), originated from two different Cretan habitats (mountainous and seaside) and grown in an open hydroponic system using perlite as substrate. Six different nutrient solution (NS) treatments were tested by combining two levels of total-N supply (4mM N or 16mM N) with three levels of ammonium to total-N supply ratio (Nr) in the NS (0.05, 0.25 and 0.5, corresponding to supply of 5%, 25%, 50% of total-N in the form of NH4-N, respectively). The results showed that the yield in terms of above-ground fresh and dry weight per plant was unaffected by NS treatments at both seed origins. However, Tavri plants showed higher dry matter content compared with that of Stavros plants. No significant differences were observed in the leaf chlorophyll content of leaves, although increasing both total-N supply and Nr in NS enhanced significantly the concentration of organically-bound nitrogen in the leaves. The concentrations of K and Ca were significantly reduced by increasing Nr from 0.05 up to 0.5. Additionally, the concentration of leaf K was further reduced by increasing the total-N supply. In contrast, the Mn and B concentrations in leaves 53 BOOK OF ABSTRACTS increased significantly with increasing Nr in NS. With respect to stamnagathi leaf quality, increasing total-N supply markedly increased leaf nitrate content (NO3-N) but the differences were significant only in Stavros. However, increasing Nr significantly decreased the nitrate content with the lowest content occurring at the highest Nr level. Interestingly, Stavros plants exhibited higher nitrate contents than those of Tavri plants at all treatments. Moreover, the results revealed a superiority of Tavri over Stavros plants in total flavonoids, phenols and antioxidant activity. T1-11 ΤHE ROLE OF A PROLYL 4 HYDROXYLASES IN TOMATO FRUIT DEVELOPMENT Panagiotis Kalaitzis1, Andreas Perrakis1, Stilianos Arhondakis1, Afroditi Krokida1, Dimitrios Kaloudas1, Craita Elena Bita1, Haytham Hamedeh1, Refca Ajmi1, Kostantinos Blazakis1 1 Mediterranean Agronomic Inst. Of Chania, 85, Macedonia Str. P.O. Box 85, 73100 Chania, Greece Proline hydroxylation is a major post-translation modification of hydroxyproline-rich glycoproteins (HRGPs) that is catalyzed by prolyl 4-hydroxylases (P4Hs). Their involvement in plant growth and development has been recently investigated in Arabidopsis, tobacco and carnation while little is known about their role in tomato. The tomato genome compromises 10 putative P4Hs with most of them being expressed during fruit development. Preliminary experiments of partial suppression of their expression using Virus Induced Gene Silencing resulted on alterations in cell division and expansion of tomato leaves. Stable transgenic tomato plants expressing a P4H3-RNAi construct showed a decrease in expression of P4H3 in fruit development and ripening. All 9 independent transgenic lines showed several phenotypes with agronomic significance: a) Fruits of smaller size due to alterations in cell enlargement. Microscopic analysis revealed a significant reduction in the final cell area caused by a significant decrease in cell enlargement; b) A reduction by 80% of the number of seeds; c) Seeds with altered morphology comprising shorter embryos exhibiting differences in germination rate; d) Alterations in the physiological and nutritional parameters of fruit ripening such as softening and carotenoid content, respectively were also observed. Collectively, these results indicate that the P4H3 plays a significant role in tomato fruit development and ripening. T1-12 CHARACTERIZATION OF THE POLYPHENOLIC PATTERN IN PRUNUS DOMESTICA VARIETIES WITH DIFFERENT FRUIT SKIN COLOR BY ANALYZING THEIR FRUIT JUICES Sofia Vio Michaelis 1, Katharina Goldner 2, Dr. Michael Neum“ller 3, Prof. Dr. Dieter Treutter 2 1 Schönmetzlerstr. , OG links, 85354 Freising, Germany 2 D“rnast 2, 85354 Freising, Germany 3 Am S“bach , Hallbergmoos, Germany Polyphenols like anthocyanins, flavan-3-ols and other flavonoids have proven to be good for human health. Therefore there is an interest in polyphenols rich fruits like plums. But the european plum cultivars show a large variability in the features of the fruit like form, size, skin color, sugar content, taste, among other. For that reason it can be expected that the poliphenolic pattern is different between them.This study aims to identify de differences in the polyphenolic pattern of european plum (Prunus domestica) cultivars with different fruit skin colors. To that purpose, fruits of 10 cultivars where harvested during 3 consecutive years. The juice was extracted with methanol using solid phase extraction. The extracts where measured with inverse phase HPLC to identify anthocyanins, flavan-3-ols, flavonols, flavones and hydroxycinnamic acids. After analyzing the extracts, two groups can be identified in relation with the total flavonoid content. One group of varieties has a higher amount of total flavonoids than the other. This 54 III International Symposium on Horticulture in Europe – SHE 2016 difference is principally influenced by the amount of hydroxycinnamic acids in the fruit juice, mainly neochlorogenic acid. Flavan-3-ol content is higher in those cultivars with higher flavonoid content. This differences where independent from the skin color, therefore from the anthocyanin presence. Flavonol and flavone content are relatively constant in all varieties. This suggests that the final amount of polyphenols is early regulated in the biosynthetic pathway. T1-13 DETERMINATION OF NITROGEN CONCENTRATION IN HYDROPONICALLY GROWN SPEARMINT Antonios Chrysargyris1, Erato Nicolaidou1, Nikolaos Tzortzakis1,2 1 Dept Agricultural Sciences, Biotechnology, Cyprus University of Technology, Lemesos, Cyprus 2 Food Science, Cyprus University of Technology, 3036, Lemesos, Cyprus The performance and suitability of spearmint (Mentha viridis L.) in nutrient solutions differing in nitrogen (N) concentrations (150-175-200-225-250 mg/l) were studied over a 6-week period in a close hydroponic system, Deep Flow Technique -DFT. Nutrient solutions with different N concentrations examined for plant growth, yield, mineral uptake, antioxidants and essential oils constitutes. Plant height reduced in 200 mg/l N compared to lower or higher N concentrations and this resulted in greater dry matter content for that N concentration. No differences were observed in leaf number, stem thickness, plant biomass, root fresh weight and root length. Physiological parameters related to Chlorophyll a, Chlorophyll b and total chlorophyll contents were greater in 250 mg/l N, while leaf stomatal conductance and leaf fluoresces did not differ among treatments. Total phenolic content and antioxidant status (FRAP, DPPH) were similar in all examined N concentrations. Plants grown in < 200 mg/l N had increased Mn, Cu, Fe and B content, while no differences were observed in macronutrients content. Essential oil main constitutes were Carvone, D-limonene, 1.8cineole and D-germacrene and varied among treatments. The results indicate that different N concentrations in the nutrient solution may affect the nutritive value and essential oil yield and constitutes for hydroponically grown spearmint. T1-14 THE USE OF LEGUMES FOR SUSTAINABLE AGRICULTURAL PRODUCTION: EUROLEGUME Dimitrios Savvas 1, Valentini A. Pappa 1, Georgia Ntatsi 1, Anastasia Tampakaki 1, Dimitrios Bilalis 1, Constantinos Kittas 2, Roikos Thanopoulos 1, Efstathia Lazaridou 1, Ioannis Karapanos 1, Anestis Karkanis 2, Ilias Travlos 1, Christos Fotiadis 1, Penelope Bebeli 1 1 Agricultural University of Athens, Department of Crop Science, Iera Odos 75, 11855 Athens, Greece 2 Department of Agriculture , Crop Production and Rural Environment, University of Thessaly, Fytokou Street, N. Ionia, 38446, Volos, Greece EUROLEGUME (Enhancing of legumes growing in Europe through sustainable cropping for protein supply for food and feed) is a 4-year international research project funded by the 7th Research Framework Programme of the European Union which started at 01.01.14. The main objective of the project is to increase the production of leguminous crops, to ensure growers in EU with varieties and to produce innovative foods and feeds. EUROLEGUME project is mainly targeted to three major legume crops, like peas, faba beans and cowpeas. The project consists of a consortium of eighteen partners (twelve research institutions, five SMEs and one SME performing research) from ten countries: Austria, Albania, Czech Republic, Estonia, Greece, Latvia, Norway, Portugal, Spain, and Sweden. The partners set as major driving force to produce results that can indeed be used by stakeholders to launch a) new food and feed products, b) new cultivars which are likely to be more adequate to the climate changes, c) high biotech products, which can be used to reduce fertilizer and energy inputs to have a more competitive legume production system, and d) overall to improve 55 BOOK OF ABSTRACTS the production area of legumes within each country and all in EU. In Greece, initial results indicated the presence of a wide phenotypic diversity among and within on-farm conserved cowpea local populations and are expected to contribute to the application of a suitable on-farm conservation method and management in accordance with different area s practices and populations structure. Moreover, data from on-going experiments revealed that the tested Greek landraces of peas and faba beans seem to have a strong influence on both GHG emissions and BNF efficiency and, therefore, appropriate selection of cultivars is imperative for efficient use of this legume plant in organic cropping systems towards an enviromentally-friendly sustainable production of European agricultural systems. T1-15 INTEGRATED PEST MANAGEMENT OF THE WHITEFLY ALEYRODES PROLETELLA IN VEGETABLE BRASSICA Ute Vogler 1, Luzian Messmer 2, Dominique Mazzi 1, Agroscope, Schloss , Postfach, 2 ET(Β, Universitätsstrasse , Wädenswil, Switzerland Β“rich, Switzerland Consumers are concerned about the nutritional value of horticultural products and environmental aspects of their production. Integrated pest management (IPM) strategies were established to ensure harvest and product quality while taking into account environmental issues. In vegetable brassica, several insect pests cause economically severe damage. The aim of this study was to evaluate measures to protect vegetable brassica from whitefly infestation, especially late in the growing season. In vegetable brassica crops, whiteflies (Aleyrodes proletella; Hemiptera: Aleyrodidae) cause severe damage by feeding and excretion, hence reducing the market value of harvested products. In field experiments, we studied the efficacy of plant protection measures in kale (Brassica oleracea var. sabellica L.) and the potential for their improvement. In semi-field experiments, we addressed the development and morphology of whiteflies with and without overwintering stimulus. Results of the field experiments reveal differences in the efficacy of insecticides to control whiteflies in kale late in the season, and point to how treatments can be improved. Results of the semi-field experiments indicate differences in development and morphology between whiteflies with and without overwintering stimulus. The results indicate challenges and opportunities for controlling whiteflies in kale late in the season. Insecticide treatments are needed to control whiteflies. Aspects of plant architecture, usage of spreading and penetration aids, as well as chemical properties of insecticides will be presented and discussed. Overwintering stimuli are related to differences between whiteflies in development and morphology. Results will be presented and discussed in terms of distinct morphs of the whitefly A. proletella. T1-16 EFFECTS OF SOME DORMANCY BREAKING AGENTS ON HORMONE CONCENTRATION DURING DORMANCY PERIOD IN FLOWER BUDS OF LAPINS SWEET CHERRY CULTIVAR Ayzin B. K“den , Burhanettin İmrak , Ali K“den , Abdulkadir Sarıer 1 University of Cukurova, Dean of the Faculty of Agriculture, Department of Horticulture, 01330 Adana, Turkey Winter chill is crucial for most of the fruit crops. Many fruit trees require a certain number of chilling, also called chill units, each winter to produce fruits in the next period. Insufficient chilling accumulation during winter period usually results as blooming disorders and decreases in fruit yield of stone fruit trees. Dormancy can be broken under certain conditions by using chemicals such as Dormex. Recent years dormex was forbidden in some countries and Turkey because it was found to be carcinogenic for the human beings. This situation raised a necessity to find alternatives chemicals to Dormex. For this aim, six years old trees of 56 III International Symposium on Horticulture in Europe – SHE 2016 Lapins trees (Prunus Avium L.) grafted on MaxMa 60 rootstocks were sprayed with 6% Erger, hydrogen cyanamide 4% (Dormex) and potassium nitrate 8% (KNO3) for breaking dormancy of the buds during 20132015 period (two years). Calculating the chilling hours below 7°C and chill unit CU values during the experimental winter periods, 511 to 576 h and 215 to 345 CU of chilling durations were obtained under subtropical conditions of Adana. The effects of dormancy breaking agents on hormonal changes especially gibberellic acid (GA3) and abscisic acid (ABA) content in floral buds were detected by using LC-DAD-ESI-MS/ Also, effect on budbreak, fruit set and fruit quality were investigated. Erger markedly advanced improved budbreak rate 55.2%, fruit-set 84.25%, quality, yield and reduced abscisic acid content in flower bud during dormant period in comparison with the other treatments and control in 2013-2014 period. 57 BOOK OF ABSTRACTS Oral Topic – 2 Quality, Authentication, Traceability & Supply Chains 58 III International Symposium on Horticulture in Europe – SHE 2016 T2-O1 PHYTOCHEMICAL CONTENTS AND OVERALL QUALITY OF LEAFY LETTUCE VARIETIES (LACTUCA SATIVA L.) GROWN IN A CLOSED HYDROPONIC SYSTEM Dharini Sivakumar 1, Beverly Mampholo 2 , Martin Maboko 3, Puffy Soundy 4, 1 Tshwane University of Technology, Staatsaritillerie Road, 0001 Pretoria, South Africa 2 Department of Crop Sciences , 0001 South Africa Pretoria, South Africa 3 ARC , Pretoria, South Africa 4 Crop Sciences, Pretoria, South Africa Lettuce consumption is determined by colour, size, texture, taste and nutritional properties. Choice of lettuce varieties is important for lettuce mixed fresh cuts or as whole product. Therefore, it is important to profile the phytochemical composition in different lettuce varieties in order to improve the antioxidant and nutrient intake in the diet. Principal component analysis illustrated three separate groups based on the bioactive compounds, colour values and leaf fresh mass for the 16 lettuce varieties. Group 1; Multired 4 contained the highest concentrations of total phenols, flavonoids (isohamnetin, quercetin, kaempferol, epicatechin, mycertin, anthocyanin , -carotene, ascorbic acid and higher antioxidant property and lower leaf fresh mass. Group 2; included the green lettuce varieties (Multigreen 3 and Multigreen 1) and the red varieties (Cantarix, Lunix, Soltero, Veneza Roxa, Feska) revealed moderately higher in bioactive compounds and leaf fresh mass. Group 3 contained the green lettuce varieties (Smile, Palmir, Hardy, Nasir, Monary, Atlantis and Vera Green Frilly) showed lower bioactive compounds and higher leaf fresh mass. Red lettuce varieties are rich in bioactive compounds and antioxidant property mainly due to the total phenols and flavonoid compounds. Multired 4, Multigreen 3 and Multigreen 1 and other red lettuce varieties can be recommended to improve the phytochemical composition in mixed lettuce salads or they can be consumed as a whole product. T2-O2 FRUIT QUALITY AND YIELD INFLUENCED BY SITE-SPECIFIC MECHANICAL FLOWER THINNING IN 'GALA' AND 'ELSTAR' ORCHARDS Michael Pflanz 1, Robin Gebbers 1, Manuela Zude 1 1 Leibniz Institute for Agri. Engineering, Max-Eyth-Allee 100, Potsdam-Bornim, Germany To achieve high fruit quality, good storability and long term stability of apple yields regulation of the crop load by reducing flowers or fruits before June drop is an important measure. Chemical thinning has been used for this purpose for many years. However, chemical thinning lacks of effectiveness, impacts the environment, and no option for tree-specific adjustment is available. Mechanical flower thinning using the Darwin string thinner has overcome many of these limitations and thus, has become a preferable alternative on apple and peaches. In particular, mechanical thinning allows the intensity to be adapted site-specifically on-the-go. This is a substantial progress towards precision fruticulture. Many cultivars show high spatial variation of flower sets from tree to tree. Nevertheless, under practical conditions, these trees are usually thinned at a uniform intensity throughout the whole orchard. This causes excessive thinning at some of the tress while several others might be left under-thinned . To resolve this problem, a new system for mechanical flower thinning was designed which combines an optical sensor for identifying the site-specific flower density, a geo-spatial decision support system (DSS) for determining the optimum thinning intensity based on the actual flower density, ancillary data (yield, soil), and a string thinning unit. Field trials have been conducted on Malus x domestica Borkh. 'Elstar' (n=220) and 'Gala' (n=200) to quantify the relation between the string thinner s rotational speed per minute rpm and the number of flowers and young fruit before June drop, the yield and fruit quality parameters from single trees of each cultivar in 2014 and 2015. 59 BOOK OF ABSTRACTS Increased yields for fruit larger 65 mm were observed for 'Elstar' in both years on trees thinned with 280 rpm. The total yield per hectare was about . and . t∙ha-1 higher compared to the un-thinned variant on sites with low soil moisture in 2014 and 2015, respectively. On adequately water supplied sites the difference was 1.5 and - . t∙ha-1 in 2014 and 2015, respectively. In terms of the fruit quality differences of soluble solids content were obtained at thinning intensities of 240 rpm for 'Elstar' in 2015. The economic impact of adaptive thinning will depend on the variability within the orchard. Based on data from one season, the yield could be improved between 5 and 10% - without regarding the benefits from reduction of alternate bearing. T2-O3 IMPACT OF LIGHT QUALITY ON ANTHOCYANIN SYNTHESIS IN WHITE ASPARAGUS SPEARS (ASPARAGUS OFFICINALIS L.) Susanne Huyskens-Keil 1, Karin Hassenberg 2, Werner B. Herppich 3 1 Lentzeallee 5557 , 14195 Berlin, Germany 2 Max-Eyth-Allee 100, D-144 Potsdam, Germany 3 Leibniz-Inst. Agricult. Eng. Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany In Germany, white asparagus is an economically important and highly appreciated crop. Nevertheless, asparagus spears are extremely perishable and, thus, reveal only limited marketability. Quality deficiencies of spears comprise textural changes, microorganism decay and red discoloration. Anthocyanin synthesis, assumed to be induced by even short-term irradiation with low light intensities, is one of the causes of undesired spear colour changes as discussed in literature (Siomos et al. 1995, Flores et al. 2003). However, other influencing factors are also reported (e.g. Flores et al. 2005, Sanz et al. 2009, Mastropasqua et al. 2016). Profound biochemical background knowledge on the ecophysiological impact on red discoloration of asparagus spears is, however, scanty and is the objective of the present study. During several years, freshly harvested white Gijlim asparagus spears were subjected to short-term (3 h) application of radiation approx. µmol m-2 s-1) of different wavelengths ranges, i.e. UV (254 nm), blue (LED, peak at 420 nm), red (LED, peak at 620 nm) and white (HQI-lamps or diffuse sky radiation). Anthocyanins contents and activities of associated enzyme as well as colour and relevant physiological parameters (rates of respiration and ethylene production) were determined before and after light treatments during d of storage at °C water saturated atmosphere) simulating retail conditions. Dark stored spears served as controls. Casual findings on light-induced anthocyanin synthesis and red discoloration of asparagus spears of the present study, which to some extent contradicts previous studies, will be discussed in detail. T2-O4 LOW RESIDUE APPLE PRODUCTION: HIGHER PRODUCTION RISK AND LOWER PROFIT Esther Bravin 1 1 Agroscope Changins-Wädenswill ACW, Postfach, Waedenswill, Switzerland The fruit production involves a high input on plant protection products (Spycher and Daniel, 2013). However, the acceptance of the use of plant protection products in horticulture is low: The market asks for a reduction of the pesticide use and ideally of the level of plant protection residue on fruits Gölles. al, . Agroscope tested during several years from a technical and economic point of view a Low Residue strategy for apple production. In 2008, a trial was started to investigate which possibilities of minimizing residues of fungicides are suitable for professional production. Three different crop protection strategies were compared: Integrated Production (IP), Organic Production (OP) and Low Residue (LR). The LR-strategy was a combination of the IP- and OP- crop protection strategy. IP-, OP- and LR-strategy have been evaluated using a multicriteria decision method assessing the economic sustainability of plant protection strategies (Mouron et al, 2012) 60 III International Symposium on Horticulture in Europe – SHE 2016 with the determination of profitability (family income per labor hour, total production cost per kg 1st class fruit, net profit per ha) and production risk (income variability, probability of dramatic yield loss). With the LR-Strategy profitability is lower and production risk is higher than with the IP- or OP- strategy. With the OP-strategy growers reach higher family income per hour and higher net profit per ha. With the IPstrategy growers have lower probability of dramatic yield loss and lower production costs per kg 1st class fruit. With this trial it was possible to develop a plant protection strategy for apple production which causes no pesticide residues. The multicriteria decision method showed that the economic sustainability of the LRstrategy without price compensation is lower than the economic sustainability of IP- and LR-strategy. A price premium for low-residue production might be justified by environmental advantages. T2-O5 CHOOSING THE OPPOSITE ' CONSUMER ATTITUDES AND THEIR CHOICE OF A HORTICULTURAL PRODUCT Sara Spendrup1, Fredrik Fernqvist2, Fanny Hkansson2, Lena Ekelund2 1 Box 88, S-230 53 Alnarp, Sweden 2 Slottsvägen , SE-230 53 Alnarp, Sweden The tomato is an essential staple of cooking and represents an important product in the fruit and vegetable category in ordinary supermarkets. Although several international studies reports that consumers express a strong preference for domestic produce, many Northern European countries show relatively low domestic market shares for tomatoes. In the case of Sweden, the share is only 13% of the total Swedish tomato market. This implies that there is an attitude-behaviour gap between expressed preferences and actual behaviour, and that other quality attributes affect consumers choice stronger than what may be predicted from stated attitudes. This paper compiles the results from two separate Swedish studies with the aim to increase the knowledge regarding consumer attitudes and choice of tomatoes in a European perspective. In the first, two quantitative surveys were performed from different points in the value chain, on retailers and consumers respectively, with the aim of finding out what arguments may apply regarding different quality attributes. Taste and product quality were among the most important attributes, and domestic tomatoes were clearly preferred to imports. In the second study, actual consumer choice was assessed in store to investigate the role of the attributes origin and packaging. Less than fifty per cent of the consumers were aware of where the tomatoes they chose came from when asked the question, and following questions showed other arguments to be of importance. Packaging was perceived as negative, for environmental reasons, but actual behaviour was here again not in line with stated attitudes. The results confirm an attitude-behaviour gap, and the existence of other factors that may be of greater importance to consumer choice. Theoretical and practical implications to the marketing of horticultural products are discussed in the paper. T2-O6 MANAGEMENT OF SANITARY AND PHYTOSANITARY RISKS IN THE APPLE INDUSTRY: THE SUSTAIN'APPLE PROJECT Jean-Marie Codron1, 1 UMR MOISA, 2, place Viala, 34080 Montpellier, France In France, apples are the first most consumed and exported fruits. The industry is under intense scrutiny from a sanitary and phytosanitary point of view due to national and European reglementation, private standards on pesticide residues, phytosanitary barriers in certain emerging countries and consumer perceptions that are sometimes critical. 61 BOOK OF ABSTRACTS Sustain'Apple aims at shedding light on the organizational and institutional solutions that may be implemented at all stages of the national chains (short and long chains) and at the international level with a focus on some crucial players (China, Chile, Italy). Priority has been given to improve the sustainability of the organizational and institutional solutions since, although tightly connected with technical solutions, they have not yet received much attention in academic and empirical literature. Sustain'Apple also evaluates the impact of some organizational and institutional solutions on the consumer perceptions of the sanitary risk, international trade and environment. The project runs over a 4 years period (2014-2017). While total funding is 3.9 million euros, it benefits from a French public subsidy of about 830k euros. The Consortium gathers all the capabilities necessary to address these sustainable apple chain issues: on the research side, INRA, CIRAD, IRSTEA, Montpellier Supagro, ESA Angers and UAPV are concerned through UMR MOISA, UMR Innovation, UMR ITAP, PSH and UERI Gotheron. The following disciplines or subdisciplines are involved : organization and institutional economics, international economics, consumer economics, strategic management, international law, agronomy, landscape ecology and Life Cycle Assessment. On the expertise side, the main contributions come from CTIFL on supermarkets, consumers, postharvest technologies and production systems, from GRAB on short organic chains and from ANPP on a detailed and global understanding of the apple production and export industry. Dissemination targets i) the public agencies in charge of SPS control, standard definition and promotion of Integrated Pest Management (IPM) solutions; ii) the main private stakeholders of the apple chains at the production, trade, distribution and consumption levels. T2-O7 THE EFFECT OF LEONARDITE, VERMICOMPOST, FARMYARD MANURE ON YIELD AND LEAF QUALITY OF GARDEN CRESS (LEPIDIUM SATIVUM L.) Nuray Akbudak1, Ozan Zambi1 1 Uludag University, Faculty of Agriculture, Department of Horticulture, Gorukle Campus, Bursa 16059, Turkey In this study, which was carried out under greenhouse conditions, investigates the effects of different combining fertilizer and soil proportion (v/v) of leonardite (1:3, 1:4, 1:8), vermicompost (1:3, 1:4, 1:8) farmyard manure (1:3) and control (soil) application on two variety of Garden Cress (Lepidium sativum L.) (cvs. Zeybek and Bahargulu) growth and leaf quality. The garden cresses were harvested on the 50 th day after seeding. Yield (kg/ da), scapus length, (cm), leaf width (cm), leaf length (cm), leaf relative water content (%), chlorophyll a (mg / g leaf), chlorophyll b (mg / g leaf), the total chlorophyll content (mg / g leaf) color, dry weight in oven were determined in the harvested plants. At the end of the study, it was determined that Garden Gress seeds did not germination in leonardit applications (1:3, 1:4). The highest yield and leaf quality was in vermicompost and farmyard applications respectively. The increasing vermicompost rates caused an increase in colour. Furthermore colour of farmyard manure is higher than vermicompost (1:4, 1:8). T2-O8 REDUCTION OF PRE-HARVEST FRUIT DROPS IN WASHINGTON NAVEL (CITRUS SINENSIS OSB.) Ilhami Tozlu1, Recep BALKIC1, Hamide Gubbuk1, Lokman Altinkaya1 1 Akdeniz University, Agricultural Faculty, Department of Horticulture, 07058 Antalya, Turkey Pre-harvest fruit drop is natural yield reducing physiological phenomenon in Washington Navel oranges. A plant growth regulator (PGR), 2,4-diklorophenoksiasetic acid (2,4-D) was commonly used chemical agent to prevent fruit drop. However; its use was banned in 2010 and search on alternative chemicals are still ongoing. Plant nutrients, boron (B; 0, 50, 100, and 150 ppm leaf application) and calcium (Ca; control and once, twice and three times of 0.5 % leaf application), and a PGR, Gibberellic acid (GA3; control, September 62 III International Symposium on Horticulture in Europe – SHE 2016 and October applications of 5 ppm), have been tested in search of an alternative chemical for prevention of pre-harvest fruit drop on Washington Navel oranges. Within the plant nutrient applications, best results were obtained from 150 ppm B and June treatment of Ca applications. On the other hand, among the GA3 applications, October application of 5 ppm GA3 has resulted in lowest fruit drop and increased marketable yield compared to that found in the other treatments. There was no difference in the calculated yield values as yield per square cm of stem and yield per canopy volume. Overall results suggest that GA3 application in October had better results compared to all other treatments. However, results of B and Ca treatments suggest that higher concentrations of these plant nutritions need to be reevaluated to have clearer understanding on pre-harvest fruit drop on Washington Navel oranges. T2-O9 PHYSIOLOGICAL, BIOCHEMICAL AND HISTOLOGICAL RESPONSE TO HIGH TEMPERATURE STRESS AT DIFFERENT STAGES OF DEVELOPMENT IN OKRA ABELMOSCHUS ESCULENTUS L. (MOENCH) Shahnoosh HAYAMANESH1, Claudia Keitel1, Nabil Ahmad2, Richard Trethowan2, Tariq Chattha2 1 380 Werombi Rd, Brownlow Hill, CAMDEN NSW, Australia 2 107 Cobbitty Road, Cobbitty, CAMDEN, Australia Okra is an important summer vegetable crop and high temperature has been shown to lower its germination, growth and yield. There is, however, limited information on where in the plant heat damage occurs and how heat stress affects Okra at different growth stages. We aimed to characterise the effect of heat stress on the physiological, biochemical and histological level and identify the developmental stages that are most vulnerable. Out of 150 genotypes, four genotypes (including heat tolerant and sensitive) from Pakistan and the World Vegetable Centre (AVRDC) were selected based on their yield and physiological response (fluorescence and electrolyte leakage) to heat shock. The selected genotypes were transplanted into control and hot tunnel houses with three replicates per genotype at two developmental stages (bud initiation and flowering . Temperatures were on average °C warmer in the heated tunnel house during the day, whereas night temperatures were similar. Heat did not damage membranes or the photosynthetic apparatus, as electrolyte leakage and fluorescence parameters were the same among treatments and developmental stages. Over 12 weeks, Okra demonstrated acclimation to heat in all physiological measurements (e.g. chlorophyll fluorescence, electrolyte leakage, assimilation rate, stomatal conductance). There were small differences in concentrations of some sugars and sugar alcohols between treatments at week 12. Heat slightly reduced above- and belowground biomass for plants transferred to heat at the bud initiation stage. Despite only small effects of heat on biochemistry and biomass, as well as physiological acclimation to heat, yield was strongly reduced as a result of damage to male reproductive tissue, including lower anther count and reduced pollen viability. T2-10 UNDERSTANDING POSTHARVEST STRESSES ON RIPENING SYNCHRONIZATION OF HASS AVOCADOS Romina Pedreschi , )gnacia (ern|ndez , Claudia Fuentealba , José Antonio Olaeta , Bruno G Defilippi 2, Reinaldo Campos-Vargas 3, Susan Lurie 4 Pontificia Universidad Católica Valparaíso, School of Agronomy, Calle San Francisco sn La Palma , Quillota, Chile 2 Insituto de Investigaciones Agropecuarias, INIA La Platina, Santiago, Chile; 3 Centro de Biotecnología Vegetal, Universidad Andrés Bello, Av. Rep’blica , Santiago, Chile 4 Department of Postharvest Science, Volcani Center, Bet-Dagan, Israel To fulfill the demand of avocados in international markets, they are transported at low temperature and under controlled atmosphere conditions over long distances and ripened at destination. Existing fruit ripening protocols at destination are inefficient, create logistical problems for marketers and result in 63 BOOK OF ABSTRACTS inconsistent fruit quality at the point-of-sale. Commonly applied postharvest abiotic stresses might decrease the ripening spread. Thus, the objectives were: (i) to evaluate the influence of different abiotic stresses (heat, controlled atmosphere (CA) and a N2 shock) on decreasing the variable ripening and (ii) to evaluate key target genes (ACS and ACC), metabolites (fatty acids) and enzyme activities (pectin methylesterase and polygalacturonase) suspected to be involved in the regulation of the ripening synchronization. The fruit flesh biopsy approach was employed to follow individual fruit ripening on early (23-26% DM) and middle season fruit (27-30% DM) with different dry matter (DM). The applied postharvest stresses consisted on: (i) CA for d at ˚C, % O and % CO ii heat treatment (T at ˚C for min followed by i and iii a N shock for h followed by i . )ndividual fruits were let to ripen at °C and % relative humidity R( . Individual fruit biopsies were taken at 1, 8, 15 and 21 days CA storage for all treatments. A total of 50 fruits per treatment were used. Five fruits with a homogenous ripening pattern were selected per treatment to understand the ripening synchronization process. Results indicate that the HT significantly reduced the observed ripening spread. Understanding of the mechanisms involved with the targeted metabolite, gene and enzyme analyses are currently being undertaken. This approach will allow the development of efficient postharvest treatments to synchronize the variable ripening of avocado batches. T2-11 THE PROPERTIES OF RICE HULL IN SOILLESS AGRICULTURE TECHNIQUE AND THE EFFECT OF PLANT DEVELOPMENT PARAMETERS IN LETTUCE (LACTUCA SATIVA VAR. CRISPA) CULTIVATION Gamze Cakirer 1, Koksal Demir 1, Cagla Temiz 1 1 Ankara University, Horticulture Department, 06110 Diskapi, Ankara, Turkey The properties of mediums used in soilless agriculture technique in plant production affect plant development directly. Mediums having different properties back up the plant in the root part and also are influential on root development, intaking water and nutrients. Peat, cocopeat and perlite mediums are the most commonly used materials in the world. However, the high cost of peat aggregate and the limited peat reserve led to seeking of alternative mediums. Rice hull, which will be one of an alternative mediums, is a material that comes off during rice processing and poses a problem in the form of waste in the environmental sense. Nowadays minimising of the problem of waste in the environmental level gains even more importance especially in sustainable agricultural system. Therefore, evaluating of rice hull, which is a renewable source, in the soilless agriculture system has a great importance. This material can be used not only without being processed but also in the forms of ground and carbonized. Studies done on the potential of using rice hull as an aggregate are not adequate. In the study, in addition to peat, cocopeat and perlite mediums which are commonly used in soilless agriculture, lettuce production was made in fresh, ground and carbonized rice hull. At the end of the study, bulk density (g/cm3), aeration capacity (%), easily available water (%), water buffering capacity (%), porosity (%), moisture (%), organic matter (%), pH and electrical conductivity values were determined in six mediums. In addition to this, by cultivating lettuce in mediums, some vegetal data were also found. In the study, it was found that rice hull which is in the form of waste can be used as an aggregate and studies should be increased to overcome the deficiencies on this subject. T2-12 WATER AND CELL WALL CONTRIBUTIONS TO APPLE TEXTURE Marc Lahaye 1 1 INRA-BIA, Rue De La Geraudiere, Boite Postale 71627, 44316 Nantes, France Defaults in fleshy fruit texture contribute to the consumer dislike of an important food component, losses in processing industries and wastes. Fleshy fruit being made up of 85-95%, its texture depends on both cellular water distribution and cell wall mechanical properties [1-3]. Assessing the respective contribution of these determinants remains a challenge but is a prerequisite for developing efficient texture control strategies. In 64 III International Symposium on Horticulture in Europe – SHE 2016 this presentation, the cell wall polysaccharides [4] and water location [5] contribution to the viscoelastic properties of apple flesh will be presented. Specific cell wall hydrolyses by purified enzymes infiltrated in Golden Delicious and Granny Smith apple, showed that pectin, hemicellulose and cellulose contributed all to the flesh viscous and elastic properties. However, it revealed that pectic homogalacturonan domain, crystalline cellulose and fucose on xyloglucan were critical structures in the viscoelastic response. Water mobility measured by non-destructive quantitative MRI experiments in texture-contrasted apples showed that vacuolar-type water was related with the elastic characteristic of firm fruit. In contrast, the proportion of water in interaction with the flesh matrix structures was related with the viscous properties of soft fruit. Chemical analysis showed that cell wall arabinose content was positively related with elasticity while xyloglucan content and structure were positively related with the viscous component. These results point to the necessity of considering together mechanisms regulating fruit cellular water fluxes and cell wall chemistry in the design of fleshy fruit texture control strategies. [1] Shackel et al. (1991) Plant Physiol., 97: 814-6. [2] Redgwell et al. (1997) Planta, 203: 162-173. [3] Wada et al. (2008) Planta, 227: 1351-1361. [4] Videcoq et al. submitted [5] Winisdorffer et al. (2015) Postharvest Biol. Technol., 104: 1-16. T2-13 TOOLS FOR STRATEGIC MANAGEMENT OF POSTHARVEST TECHNOLOGY: AN APPLICATION TO THE PORTUGUESE PEAR INDUSTRY Domingos Almeida 1 1 Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal Technology management is an essential part of business planning. Rapid technological turnover and regulatory changes, such as the ban on chemicals for postharvest applications, can disrupt equilibria within supply-chains and challenge the fruit business in a context of intense international competition. A recent example was the ban on diphenylamine (DPA), which had been the standard technology to mitigate superficial scald and internal browning disorders during long-term storage of Rocha pear. )n , the economic impact of the ban on DPA on the Portuguese pear industry was estimated in 22 to 39 million euros, i.e. 16 to 28% of the crop value (base: 2007-2009 average), and the industry was caught without alternatives. This example highlights that technology is an external variable for the horticultural industry and adequate transitions between technologies require surveillance for adequate timing and a good understanding of technological curves. As a consequence, a suitable set of tools for strategic management of technology was developed in July of 2015 and applied in a workshop with participants representing more than 60% of the Portuguese pear production. Participants were asked to list the postharvest technologies most relevant to their business. After this open list, the concepts underlying technology management were explained and the participants were guided through a series of templates in order to sequentially identify i the company s technology portfolio, (ii) a technological matrix to position each technology according to its competitive impact and the degree to which the company is skilled dealing with the technology, (iii) position the technology in its life cycle, (iv) list five technologies that should object to continuous technological surveillance and, finally, (v) develop a technology roadmap. At the end of the workshop a new tool kit for strategic management of technology was made available to the individual companies and a sector-wide assessment was produced. This communication will present the tool kit and the results obtained. In brief, postharvest fungicides, grading, and packaging systems were considered base technologies, those that are dominated by the company but no longer provide competitive advantages. New storage technologies, namely, dynamic controlled atmosphere, were considered key technologies, i.e. those that are likely to have large competitive 65 BOOK OF ABSTRACTS advantages for the sector. Finally, nondestructive methods for quality assessment, edible coatings, water disinfectants, and novel fungicide formulations were considered the main emerging technologies that require surveillance. Awareness of the technological changes by businesses are also discussed. T2-14 PRE-HARVEST RED LIGHT EMITTING DIODE TREATMENT EFFECTS ON NITRATE REDUCTION IN GREEN VEGETABLES Akvile Virsile1, Ausra Brazaityte1, Giedre Samuoliene1, Ingrida Odminyte2, Ramunas Sirtautas1, Jule Jankauskiene1, Sandra Sakalauskiene1, Viktorija Vastakaite1, Pavelas Duchovskis1 1 LRCAF Institute of Horticulture, 30 Kauno str. , LT-54333, Kaunas distr., Babtai, Lithuania 2 Vytautas Magnus University, 8 Vileikos str. , LT-44404 Kaunas, Lithuania The objective of this study was to validate the effect of pre-harvest red light emitting diode (LED) irradiation on nitrate reduction in different green vegetables. The nitrate, nitrite contents and their reducing enzyme (nitrate and nitrite reductases) activities were measured in corn salad (Valerianella locusta 'Vit'), tatsoi Brassica narinosa 'Rozetto F and amaranth Amaranthus tricolor 'Red Army' . Plants were grown in polycarbonate greenhouse / °C day/night temperature under natural illumination, supplemented with high pressure sodium lamps (PS; µmol m-2s-1, 16 h photoperiod). The effects of short-term (1-7 days) pre-harvest red nm LEDs irradiation µmol m-2s-1, 16 h) treatment were investigated. Results revealed that red LED light stimulates reduction of nitrates to nitrites and this effect depends on the duration of irradiation and on plant genotype: the nitrate contents were less affected by light in corn salad, but the effect was more expressed in tatsoi and amaranth, which were characterized by higher overall sensitivity for applied light treatment. The red LED effects on nitrate and nitrite contents correlated with nitrate reductase and nitrite reductase enzyme activities, indicating immediate light effect on nitrate reduction processes. Red LED light short-term pre-harvest treatment was shown to be an efficient tool for reducing nitrate contents in green vegetables; however, the extent of the effectiveness depends on the application season and plant genotype. T2-15 EFFECTS OF ACTIVE MODIFIED ATMOSPHERE PACKAGING WITH ARGON AND NITROGEN ON POSTHARVEST QUALITY OF LOQUAT Ayse Tulin OZ1, Ebru Kafkas2, Tulin sahin1 1 Osmaniye Korkut Ata University, Food Engine, 80000 Osmaniye, Turkey 2 Cukurova University, Adana, Turkey Active modified atmosphere packaging MAP polyethylene μm with four different gas compositions % O2 + 3 % CO2 + % 85 Ar), (12 % O2 + 3 % CO2 + % 85 N), (15% O2 + 5% CO2 + % 80 Ar), (15 % O2 + 5% CO2 + % 80 N) and control (0.03% CO2 + 20.8 % O2) were applied. To monitored postharvest quality and shelf life of Ottawianni loquats fruits at OC with a relative humidity of % during days. Postharvest quality parameters of Ottawianni loquats fruits such as skin browning, fruit skin color L*, a*, b* values, fruit flesh firmness, total soluble solids (TSS), titratable acidity (TA), ratio of TSS/TA, pH total phenolic content (TPC), total flavonoid content (TFC), organic acids and soluble sugars and percentage of O2/CO2 changes were evaluated. Results indicated that argon treated fruits both TPC and TFC were higher compared to other treatments. However nitrogen application kept fruit skin color more green than other treatments. The present findings show that active MAP storage including different headspace gas concentrations was found to be effective in maintaining the postharvest quality of Ottawianni loquats fruits for days of storage. 66 III International Symposium on Horticulture in Europe – SHE 2016 T2-16 SANITARY MANAGEMENT PRACTICES IN THE FRENCH FRUIT PRODUCTION Zouhair Bouhsina 1, Magali Aubert 1, Geoffroy Enjolras 2, 1 Inra UMR Moisa, 2 Place Pierre Viala, 34060 Montpellier, France 2 IAE - CERAG, 525 Avenue Centrale, 38400 Saint-Martin-d'(ères, France Recent sanitary crises have strengthened the requirements of consumers and politicians in terms of health risk management. Thus, the whole chain has been impacted, from the producer to the consumer. As the first link in the chain, the producer is a key player. His practices condition indeed the phytosanitary quality of production. France is one of the European countries for which phytosanitary requirements are the highest. Fruit production is a relevant sector to be considered insofar as fruits represent less than 1% of the agricultural area but more than 5% of the phytosanitary expenditures. With an amount of pesticides expenses close to € per hectare, this sector is the most intensive consumer of pesticides. The core objective of the study is to study the practices of French fruit producers in terms of biological control and also of use of phytosanitary products, while considering the relationship between these two kinds of practices. To that aim, the analysis is based on an "Orchard Survey" Enquête Vergers , carried out by by the French Statistical and Forecasting Service (SSP). This survey, which is representative of all French farms producing fruits, allows to appreciate both the degree of use of phytosanitary products (through the number of treatments) and the implementation of alternative practices (measured by the number of auxiliaries). The use of biological control techniques is designed to reduce the use of phytosanitary products. The effectiveness of this relationship is verified in practice by estimating an econometric modelling based on simultaneous equations, which considers the extent to which the implementation of biological controls influences the level of phytosanitary use and reversely to what extent the use of chemicals influences the implementation of biological control techniques. Preliminary results tend to confirm that the intensity of phytosanitary treatments decreases once farms are involved in biological control. T2-17 EFFECT OF CONVENTIONAL AND ORGANIK FERTILIZATION ON YIELD AND FRUIT QUALITY OF POMEGRANATE (PUNICA GRANATUM L.CV. HICAZNAR) Keziban Yazici , Alpaslan Sahin , Cevdet Fehmi Özkan , Bet“l Sayin , Ali Öztop , Elif )sil Demirtas , Serife Bilge Göl“kç“ , Esin Ataseven Isik2 1 Recep Tayyip Erdogan University, Faculty of Agriculture and Natural Science, Horticultural Department, Rize, Turkey 2 West Mediterranean Agricultural Research In, Antalya, Turkey In this study, conventional and organic production systems for Hicaznar pomegranate cultivar, which is widely cultivated with a good export potential in Turkey, were compared. This study was conducted in West Mediterranean Agricultural Research Institute in 2004 and 2008 by allowing distance between conventional and organic parcels with three replication and each replication consisted of four trees. The aim of this research was to evaluate the effect of organic and inorganic fertilizers on quality and the response of Hicaznar to diseases and insects. Fertilizer programs for conventional and organic production have been optimized and applied after analyzing leaves and soil characteristics for each parcel. Green manure crop, green manure crop supplemented with organic fertilizer and green manure crop supplemented with turf have been used in organic pomegranate production. Effects of different treatments on quality attributes of fruit and the average yield per tree were determined. According to our results, the highest yield rate and quality were obtained with conventional and green manure crop plus organic fertilizer applications, respectively. 67 BOOK OF ABSTRACTS T2-18 INCREASED PHOSPHORUS USE EFFICIENCY IN BASIL GROWN HYDROPONICALLY WITH LOW PHOSPHORUS CONCENTRATION AND SALINE WATER Daniele Massa1, Gianluca Burchi1, Luca Incrocci2, Rocco Germano2, Giulia Carmassi2, Alberto Pardossi2 1 Via dei Fiori, 8, 51012 Pescia (Pistoia), Italy 2 Viale delle Piagge, 23, Pisa, Italy Phosphorus is one of the most important nutrients in agriculture for its physiological, ecological and economic implications. Phosphorus reserves are decreasing around the globe and therefore its price has been increasing in the last years. Phosphorus is subjected to many chemical phenomena in the root zone that make its management in cropping systems more and more difficult. In soilless culture, P can be monitored and managed with high precision in order to improve its uptake by roots and minimize its losses. In this experiment, basil (Ocimum basilicum L.) was grown hydroponically with different P (H2PO4-) and NaCl concentrations in the nutrient solution i) to evaluate the effects of P and salinity on plant yield and mineral nutrition, and ii) to verify the possibility to decrease P concentration without growth reduction. Three P concentrations (i.e. 1.0, 0.5, and 0.25 mol m-3) were combined with increasing NaCl concentrations (i.e. 0, 15, and 30 mol m-3) in the root zone. Plants faced the low concentration of P in the nutrient solution by increasing the root/shoot ratio. Plant yield was not influenced by P and NaCl levels. Water uptake decreased with increasing NaCl concentration but at lower extent when plants were grown at low P concentration. Apparent P uptake concentration (i.e. the ratio between P and water absorbed by the plant) increased with P levels in the nutrient solution. These findings suggest that basil can be grown hydroponically at P concentration much lower than those currently used in commercial operations. T2-19 CONFIGURATION OF BABY LETTUCE QUALITY GROWN IN FLOATING SYSTEM IN RESPONSE TO CULTIVAR, CUT NUMBER AND SALINITY SOURCE Youssef Rouphael 1, Francesco Napolitano 1, Giampaolo Raimondi 1, Antonio Colantuono 1, Paola Vitaglione 1, Stefania De Pascale 1 1 Via Universit{ , Portici,)T, Portici Napoli , Italy Minimally processed or fresh-cut leafy vegetables have been gaining importance in the worldwide vegetable market in recent years. Consumer demand has been increasing and many farmers have oriented their production plans towards baby leaf vegetables such as lettuce (Lactuca sativa L.). The management of mineral nutrition is a key pre-harvest factor determining the yield and quality of leafy vegetable crops. From this perspective, the floating system, represents an important tool, since it provides the possibility of regulating secondary metabolism by proper control of the nutrient solution composition. Another cultivation practice that may affect the quality of leafy vegetables is the number of cuts. Baby lettuce is cut several times (2 or 3) during the growing cycle, leading to physiological changes that affect the plant and obviously the obtained product. The aim of the current research was to assess in a multi-factorial approach the effects of the number of cuts and nutrient solution management in particular three chloride salts (NaCl, KCl and CaCl2) tested at equimolar concentrations on yield, mineral composition, nitrate content and bioactive compounds of two baby lettuce cultivars (red or green salad bowl). NaCl and KCl treatments reduced biomass production, while similar values were recorded in CaCl and control treatments. The Green Salad Bowl exhibited higher yield and dry matter, whereas the Red Salad Bowl was characterized by high content in antioxidant activity, phenolic acids and vitamin C. Irrespective of treatments, chlorogenic acid and cichoric acid were the most abundant phenolic acids in both green and red lettuce. The application of CaCl2 produce leaves of lettuce with high quality: highest caffeoyltartaric and cichoric acid and the lowest nitrate contents. Our results also demonstrated that the second cut influenced positively the mineral composition (P, K, Ca, and Mg) and nutritional quality (e.g. phenolic acids) of baby lettuce. 68 III International Symposium on Horticulture in Europe – SHE 2016 T2-20 BRAND AWARENESS INCREASES LIKELINESS TO BUY BRANDED HERB AND VEGETABLE TRANSPLANTS Bridget Behe 1, Patricia Huddleston 2 1, Michigan State University, Department of Horticulture, A288 Plant & Soil Sciences Bldg., East Lansing, MI 48824-1325, United States of America 2404 Wilson Road, Dept. of Communication Arts Sciences, Michigan State University, East Lansing Michigan 48824, United States of America Marketers invest nearly 8% of their advertising budget on in-store marketing because > 70% of all buying decisions are made at the point of purchase. Older consumers, especially Baby Boomers (typically classified as persons aged 50-64 years) have long been considered a core target market for ornamental horticultural products. However, some industry concerns have arisen with regard to the lack of purchasing among younger age cohorts, especially Gen X (ages 38-49 years) and Gen Y (ages 25-37). Brands help create the perception of added-value while also differentiating products from competitors. Often, brands are one of a few pieces of information consumers use to make product choices. We conducted an online survey in May 2014 to investigate the role of age cohort and brand recognition on the likeliness to buy (LTB) of two herb and two vegetable transplants. We showed study participants images of 16 plants, varying the container color (white, green, and yellow), plant type (basil, parsley, tomato, and pepper), plant brand (generic and 3 national brands), and price. A total of 566 responses came from the entire U.S. and consisted of only people who had made at least one plant purchase. We divided the sample into three age groups: Gen Y (ages 18-29), Gen X (ages 30-49) and Boomers (ages 50 and older) to see if there were differences between them. Overall, we saw the highest brand recognition for Brand T (the youngest brand), followed by Brand R then Brand S (the oldest brand). A higher percentage of Boomers had seen Brand T compared to Gen Y and Gen X. However, a larger percentage of Gen X and Gen Y had seen Brand R. The percentage of respondents from each age group who had seen Brand S was similar. Both Brand R and S appear primarily on vegetable and herb transplants whereas Brand T primarily markets flowering plants. We also compared the average likelihood to buy for branded and non-branded plants by age group. Overall, branded plants were preferred over unbranded plants, with a higher average likelihood to buy rating even though the plants were digitally identical. In addition, we found that average likelihood to buy was higher for the Gen X and Gen Y groups compared to Boomers. Thus it would appear that the national brands studied here did create a perceived difference in the minds of the Gen X and Gen Y participants of this study. We then compared the average likely to buy score for each brand and each age group by whether the participant had seen the brand logo (before the study). Participants who had seen the brand logo prior to the study reported a higher likelihood to buy average score for all three brands study. We found an interaction between age and brand recognition, however, the pattern of mean LTB was similar for all three brands in the study. Gen X and Gen Y were more LTB the branded plants they had seen, with Boomers exhibiting a similar reaction but also reporting a lower LTB whether or not they had seen the brand. 69 BOOK OF ABSTRACTS Oral Topic – 3 Genetic Resources, Systems Approaches & Biodiversity 70 III International Symposium on Horticulture in Europe – SHE 2016 T3-O1 GENOME WIDE ASSOCIATION STUDIES AND WHOLE TRANSCRIPTOMIC SURVEY DECIPHER THE FRUIT TEXTURE REGULATION IN APPLE TOWARDS THE SELECTION OF NOVEL SUPERIOR ACCESSIONS Mario Di Guardo1, Brian Farneti1, Nicola Busatto2, Massimo Delledonne3, Walter Guerra4, Thomas Letschka4, Lidia Lozano4, Riccardo Velasco1, Eric Van de Weg5, Marco Bink5, Fabrizio Costa1 1 Via Mach 1, 38010 San Michele all'Adige, Italy 2 Viale Fanin 46, 40100 Bologna, Italy 3 Strada le Grazie 15, 37134 Verona, Italy 4 Laimburg 6, 39040 Ora, Italy 5 PO Box 16, 6700 AA Wageningen, Netherlands The quality of fruits is represented by the combination of important genetically controlled features undergoing a continuous variation throughout the entire fruit development till the end of the ripening process. Amongst all the fruit quality traits, texture plays a pivotal role in apple for its direct impact on both quality perception and postharvest performance. In order to decipher its regulation, a multidisciplinary approach was employed. As first attempt to dissect the fruit texture genetic architecture, two strategies were implemented, with the final goal to target the major QTLs involved in this control. Initially, a multi-families pedigreed scheme, known as Pedigree Based Analysis (PBA), was used. In this experimental design, six fullsib populations were phenotyped with a sophisticated novel texture analyzer for a high-resolution characterization of fruit texture, further genotyped with a 20K SNP Infinium Illumina Array. The joint analysis of these two dataset through a Bayesian statistics identified a series of genomic regions related to both mechanical and acoustic signatures. The intervals defined here were moreover validated with a Genome-Wide Association Study, exploiting the same genotyping and phenotyping systems on a large apple collection. In addition, the fruit texture physiology was assessed with whole transcriptomic tools. Two types of microarrays were in fact designed and exploited in this survey, unraveling the functional machinery ongoing during the fruit life cycle, especially with regards to the plant hormone ethylene. The combination of these resources provided an inestimable source of information, essential to step forward in the general comprehension of the genetics and physiology of apple texture, elucidating, in parallel, its relationship with other important fruit quality traits. This knowledge would allow, in a close future, a more accurate and precise selection of the most favorable new apple accessions distinguished by valuable and superior fruit quality. T3-O2 USE OF MARKER ASSISTED SELECTION METHODS IN TOMATO BREEDING IN BELARUS Aliaksandr Kilcheuski1, Nalliya Nekrashevich1, Olga Babak1, Sergey Malyshev1, Irina Zaitseva2, Irina Pugachova2, Mikhail Dobrodzkin2 1 Akademicheskaya Street 27, 220072 375 Minsk, Belarus 2 Michurin Street 5, 213407 375 Gorki, Mogilev Region, Belarus We have developed and used the methods for DNA identification of fruit quality genes nor, rin, norA (long shelf life), og, Del, r , hp-1, hp-2dg, B from S.pennellii, ogc, t, gf-3 (increased carotenoid content), genes determining the growth type of main and lateral shoots (sp, ls1, ls2, bl-1 and bl-2) and the techniques known from scientific literature for resistance genes to Cladosporium fulvum (cf-2, cf-4, cf-5, cf-9), to Fusarium oxysporum (I-2), to Meloidogyne incognita (Mi-1.2), tomato mosaic virus (Tm2), to high anthocyanin content gene (Ant1) in order to identify the breeding material according to the allele composition. Based on traditional and molecular marker techniques we have initiated Belarusian breeding programmes aimed at producing tomato hybrids combining a number of valuable alleles. Topcross and diallel cross schemes were tested under field and greenhouse conditions. The forms with interested allele combinations were selected in the F2 generation, using МАS methods. Now the unique model tomato forms with a complex of the fruit quality and disease resistance genes and also genes determining growth 71 BOOK OF ABSTRACTS specificity of the main and lateral shoots are used for the development of valuable hybrids. As a result of our investigation, F1 hybrids 'Sapsan' and 'Storage' (2010), 'Bubenchik' (2011), 'Tainik'(2012), 'Agenchik' (2013), 'Irma'(2014) were included in the State Registry of Cultivars, Trees and Shrubs Species of the Republic of Belarus. T3-O3 HIGH PERFORMING PHENOMICS OF BERRIES AROMA BY PTR-TOF-MS Brian Farneti1, Iuliia Khomenko1, Marcella Grisenti1, Alberto Algarra1, Matteo Ajelli1, Paula Poncetta1, Luca Cappellin1, Lara Giongo1, Franco Biasioli1 1 Via Mach 1, 38010, San Michele all'Adige TN, Italy Aroma is one of main factors impacting fruit and vegetables quality and consumer appreciation. Since aroma involves the perception of a pletora of volatile organic compounds (VOCs), their assessment is crucial to guarantee the selection and marketability of high quality fruits. High priority should thus be given to replacing poor flavor cultivars with favorable ones, exploiting the variability already available in nature. However, the analysis of the aroma trait in a large number of samples, necessary to overcome the usually massive biological and genetic variability among samples, may be laborious and time consuming. The application of Proton Transfer Reaction -Time of Flight- Mass Spectrometry (PTR-ToF-MS) has been recently described as a powerful high performing phenotyping tool for both genetic and quality related studies. This contribute describes the application of PTR-ToF-MS for the study of the aroma variability present in the germplasm collection of the main berries species available at the Foundation E. Mach, with particular regard to strawberry (Fragaria x ananassa), raspberry (Rubus), and blueberry (Vaccinium). The rapidity and the moderate cost of PTR-ToF-MS analysis, coupled with a multipurpose autosampler, allowed us to perform a detailed aroma characterization of each species with a peculiar attention to the VOC fold changes caused by ad hocstorage experiments tailored to simulate the from farm to fork chain. These results confirmed that PTR-ToF-MS can be easily implemented into the breeding phase as a tool to phenotype berries aroma T3-O4 STATUS OF RESEARCH, BREEDING AND PROTECTION OF CUCURBITS IN RELATION TO CUCURBIT DOWNY MILDEW: THEIR LIMITS AND PERSPECTIVES Ales Lebeda 1, Eva Kristkova 2, Bozena Sedlakova 2, Mark Widrlechner 3 1 Palacky University in Olomouc, Faculty of Science, Dept. Botany, Slechtitelu 11, 783-71 Olomouc-Holice, Czech Republic 2 Palacky University in Olomouc, 783 71 Olomouc, Czech Republic 3 Iowa State University, Ames 50011, United States of America Knowledge of the recent distribution, host range, virulence, DNA profiles, and resistance to fungicides of Pseudoperonospora cubensis (Berk. & M.A. Curtis) Rostovzev, and sources of host resistance to this pathogen are reviewed. P. cubensis is widely distributed in all continents, with a high capacity to adapt to changing environmental conditions and new regions. Its host-plant range includes more than 60 species and 20 genera of Cucurbitaceae. P. cubensis exhibits clear host specialization, with shifts in host range and an increasing spectrum of new host species. Temporal shifts to higher virulence levels and more complex virulence patterning within pathogen populations has been recorded in the Czech Republic, Israel (in 2002), and the USA (in 2004). Pathotypes have been described, and a triple-tetrade code proposed for their denomination. A differential host set for racial determination and denomination is under development. Forty-five different reaction patterns were recorded from the interaction of 115 Cucumis melo accessions to 8 isolates of P. cubensis with different levels of virulence; most C. melo accessions were highly susceptible to all isolates; but PI 315410 (VIR 5682) was resistant to 5 isolates. ecause of race-specificity in host-pathogen interactions, 72 III International Symposium on Horticulture in Europe – SHE 2016 resistance breeding is rather difficult and resistance typically not stable. In-vitro tests of fungicide efficacy in 2001 – 2014 documented high levels of efficacy in fosetyl-Al (Aliette 80 WP) and propamocarb (Previcur 607 SL) to Czech strains of P. cubensis, with increased resistance of some strains, inefficacy of metalaxyl, metalaxyl-M and cymoxanil (Ridomil Plus 48 WP, Ridomil Gold MZ 68 WP, Curzate K), and inefficacy of dimethomorph (Acrobat MZ) with a temporal shift from resistant to tolerant and sensitive isolates. Changes in the genetic structure of populations of 67 P. cubensis isolates collected in the Czech Republic and other European countries during 1995 – 2012 were detected by Multilocus sequence analysis (MLSA) of four mitochondrial and two nuclear DNA regions. These changes may be associated with recently reported shifts in virulence. Data on host-range changes, spatio-temporal variability in virulence and fungicide resistance, confirm the high micro-evolutionary potential of P. cubensis populations and more detailed research is required for a better understanding of these host-pathogen interactions as well as for more efficient disease management. T3-O5 METABOLIC PROFILING OF SUN-DRIED GRAPES DESTINED FOR THE PRODUCTION OF `COMMANDARIA´ DESSERT WINE Savvas Constantinou2, Vlasios Goulas , Ana Mari Gómez-Caravaca3, Antonio Segura-Carretero3, George Manganaris1, 1 Anexartisias 33, P.O. Box 50329, 3603 Lemesos, Cyprus 2 Anexartisias 57, Lemesos, Cyprus; 3 Research and Development of Functional Food, Granada, Spain Grapes sun-dried process is one of the most important steps in the production of Commandaria , a protected designated origin (PDO) product exclusively produced in Cyprus from the two indigenous grape cultivars, namely Mavro and Xynisteri . )n the current study, the effect of sun drying process on the conventional enological parameters and phenolic profile of grapes of the aforementioned cultivars was dissected. Grapes of the examined cultivars were analyzed at harvest and at the end of sun-drying process that corresponds to ca. 35-40% water loss. Results highlighted significant differences in chemical composition of the grapes before and after sun-drying process. Apart from the increase in soluble solids content, a significant increment in glucose, fructose, total acidity, total phenols and total flavonoids contents was monitored. Subsequently, individual phenolic compounds analysis was conducted with the employment of LC-DAD-qTOF-MS; polyphenolic fingerprint of the two indigenous cultivars consisted from forty-three phenolic compounds. Results alsoindicated that changes in the phenolic composition of the grapes are not only correlated with the concentration effect, but synthesis or degradation reactions also occurred. In particular, the increment in the concentration of hydroxybenzoic acid contents was higher than the concentration effect for both cultivars. Regarding to hydroxycinnamates, the dehydration caused a six-fold increase of hydroxycinnamic acid content in both cultivars; whereas the concentration of some hydroxycinnamic acids such as caffeic acid dihexoside and fertaric acid isomer decreased after sun-drying. The sun-drying process also induced stilbene and lignin synthesis production. In addition, a significant effect of dehydration on three groups of flavonoids, namely flavonols, flavan-3-ols and flavanonols. 73 BOOK OF ABSTRACTS T3-O6 ADVANCED MATHEMATICAL ALGORITHMS TO CHARACTERIZE OLIVE VARIETIES THROUGH MORPHOLOGICAL PARAMETERS Konstantinos Blazakis1, Luciana Baldoni2, Abdelmajid Mouk3, Marina Bufacchi4, Panagiotis Kalaitzis1 1 Mediterranean Agronomic Institute of Chania, Dept.of Horticultural Genetics Biotech, Makedonias 1, 73100 Crete Chania, Greece 2 Italian National Research Council, Institute of Biosciences and Bio-Resources , Via Madonna Alta, 06128 Perugia, Italy )NRA Marrakech, UR Amélioration des Plantes, Marrakech, Morocco 4 Italian National Research Council, Institute for Agriculture and Forest System, Via Madonna Alta, 06128 Perugia, Italy The morphological analysis of olive fruits, leaves and stones may represent an efficient tool for the characterization and discrimination of varieties and the establishment of phenotypic relationships among them. In recent years, much attention has been focused on the application of DNA molecular markers due to their high capacity to efficiently and reliably discriminate cultivars. In this talk, we will present a semiautomatic methodology of detecting various morphological parameters based on image analysis tools. A number of morphological parameters have been used to characterize olive germplasm collections from different countries. The data obtained could complement olive databases comprised of genetic, molecular and morphological data supporting effort to efficiently discriminate them and infer either genetic and/or morphological relations. With the aid of computing and image analysis software, we created semi-automatic algorithms applying intuitive mathematical descriptors that quantify many fruit, leaf and endocarp features. In particular, we examined quantitative and qualitative characters such as size, shape, symmetry, surface roughness and presence of additional structures (nipple, petiole, etc.). Finally, in this talk we will present the efficiency and robustness of the proposed methodology for the description of other crop morphologies such as tomatoes, pears, grapes, etc. T3-O7 MSAPANALYZER: AN ONLINE TOOL FOR ASSESSING METHYLATION SENSITIVE AMPLIFIED POLYMORPHIC (MSAP) MARKERS BY COMPARISON TO REFERENCE STATES ndreas Doulis 1, Anastasios Oulas 3, Georgios Pavlopoulos 4, Evangelia Vogiatzaki 2, Konstantinos Karantemiris 2, Theodora Kapari-Isaia 5, Eytichios Protopapadakis 6 1 Hellenic Agricultural Organisation DEMETER, Institute for Olive Tree, Subtropical Crops, P.O. Box 2228, GR-71003 Heraklion, Greece 2 Hellenic Agricultural Organisation DEMETER, Heraklion, Greece 3 Institute of Marine Biology, Biotechnology , Heraklion, Greece 4 Division of Basic Research, School of Medicine, Heraklion, Greece 5 Agricultural Research Institute ARI, Nicosia, Cyprus 6 Sofokli Venizelou 79, Chania, Greece MSAP methodology has been established in order to detect genome-wide epigenetic variation in the form of DNA methylation polymorphisms of anonymous sequences and allows for a first estimate of DNA methylation changes when scarce species-specific genomic information is available. Here we present a web tool that streamlines data processing and improves efficiency of the methylation sensitive amplified polymorphic (MSAP or MS-AFLP markers. With the help of the present tool MSAP analyser , a series of treatment states can be compared to a reference state. For each individual marker detected, a comparative profile is produced and assigned to one of the sixteen possible methylation change patterns. The tool produces a summary of demethylation, de-novo methylation, other, or no-change events and provides descriptive statistics and tests along with PCA analysis and visualization and NJ/UPGMA hierarchical clustering in the form of dendrograms. MSAPAnalyser significantly minimizes manual data processing and error handling. The new tool was initially tested by analyzing novel Citrus rootstocks which were clonally propagated, in vitro, under different hormonal treatments. Four (4) primer combinations were employed for the analysis of eighteen (18) samples following processing with EcoRI and HpaII/MspI. 74 III International Symposium on Horticulture in Europe – SHE 2016 T3-O8 SUSSEBTILIBY OF SEO/HARLAYNE TYPE RESISTANT CULTIVARS TO PPV-T Kahraman G“rcan1, Saffet Tebber1, Uurtan Kadir Ylmaz Erciyes University, Bet“l - Ziya EREN Genome and, Stem Cell Research Center, 38039 Kayseri, Turkey 2 Erciyes University, Faculty of Agriculture, Departmant of Horticulture, 38038 Kayseri, Turkey; ugurtan@erciyes.edu.tr Plum pox virus (PPV) is the most detrimental disease of the Prunus species. The use of genetic resistance is the most promising solution for the control of the virus. To date, the resistance has only been found in the North American apricot germplasm. The American cultivars Stark Early Orange SEO , Goldrich , (arlayne , Stella , and (arcot are known as a resistant against to PPV-D and M strain, both of which are the most common strains in Europe. These cultivars host a major locus located on the Linkage Group One (LG1) accounting for 70% of PPV resistance. In a previous study, Turkish apricot germplasm were screened for three markers which are known to be linked to the resistance locus on LG1 and 5 accessions in Turkish collection were identified to host the markers. Here we report the first two years results of the biological test applied to the same collection. Biological test was commenced in Kayseri in 2012 by graft inoculating the accession with a PPV-Turkey strain. After two years examination, we observed that SEO, Harlayne, Harcot and the 5 accessions of Turkish apricot germplasm with resistance markers fails to show resistance to PPV-T. The result indicates that stone fruit breeding programs needs reconsider breeding strategy noticing that PPV-T breaks the resistance in apricots. T3-O9 A MULTIDISCIPLINARY APPROACH REVEALS NEW ASPECTS OF APPLE SUPERFICIAL SCALD ETIOLOGY AND COLD RESISTANCE MECHANISM IN GRANNY SMITH FRUIT Nicola Busatto 1, Brian Farneti 2, Massimo Delledonne 3, Urska Vrhovsek 2, Franco Biasioli 2, Riccardo Velasco 2, Guglielmo Costa 1, Fabrizio Costa 2, 1 Department of Agricultural Sciences, University of Bologna, Bologna, Italy 2 Fondazione Edmund Mach, San Michele all'Adige, Italy 3 Department of Biotechnology, University of Verona, Verona, Italy Chilling injuries represents a major issue in post-harvest management. The usage of low temperature as strategy to extend storage capacity of climacteric fleshy fruits leads to the development of many physiological disorders, which seriously affect the entire marketability. The main symptom of superficial scald is represented by a diffuse browning coloration, generally limited to the skin and the underlying six-cell layer, occurring after the re-establishment of room temperature conditions. In this work, RNA-seq technology was employed to characterize the transcription dynamics of Granny Smith apples affected by superficial scald and treated with 1-MCP (1-methylcyclopropene), an ethylene competitor known to interfere with this physiopathy. In addition to this detailed transcriptomic overview, a complete metabolomic profiling was performed in order to determinate robust correspondences between gene expressions and phenotypic traits (sugar content, polyphenol accumulations and VOC productions). With the aim of understanding the etiological cause of superficial scald and the consequence of 1-MCP application on the scald progression, particular efforts were dedicated to decipher the effect of 1-MCP on the fruit transcriptomic pattern. Early evidences suggested the capacity of 1-MCP to stimulate the synthesis of sorbitol, trough the up-regulation of two Sorbitol-6-Phosphate Dehydrogenases (S6PDH) genes, representing the two most differentially expressed elements in the comparison between treated (unaffected) and untreated (affected) fruit. Both sequences were further isolated, validated by sequencing and cloned in an expression vector under the control of the CaMV-35S constitutive promoter. This construct was used to generate several 35S::S6PDH Arabidopsis transgenic lines used to assess the protective effect of the S6PDH overexpression against chilling injuries. Both transformed and wild-type plants were grown under standard conditions and then placed at – . °C for two days. Following the induction of the cold stress, a higher survival rate of the overexpressing lines were observed, indicating a possible role of sorbitol in scald prevention. 75 BOOK OF ABSTRACTS T3-10 COST ACTION EUROCAROTEN: EUROPEAN NETWORK TO ADVANCE CAROTENOID RESEARCH AND APPLICATIONS IN AGRO-FOOD AND HEALTH Antonio J. Meléndez-Martínez Laboratory of Colour & Food Quality, Department of Nutrition and Food Science, Universidad de Sevilla, Spain Carotenoids are widespread isoprenoid secondary metabolites that are involved in many actions in Nature. They are biosynthesized by all photosynthetic organisms, some non-photosynthetic bacteria and some fungi. With very few known exceptions, animals cannot synthesize them de novo, although they can acquire them through the diet and even modify them. They are very important in the context of food, nutrition and health. Thus, they do not only provide yellow, orange and red colours, but also some of them can be metabolized into retinoids with vitamin A activity. Furthermore, diets rich in carotenoid-containing foods have been associated to a reduced risks of developing serious diseases (like certain types of cancer or age-related macular degeneration, among others). They are also thought to be involved in the protection against oxidation and mental decline. Over 700 carotenoids have been described so far, although only 10-20 are being thoroughly studied. In this context, the COST Action EUROCAROTEN is aimed at advancing carotenoid research and applications in agro-food and health. The key research question is: what novel sources of carotenoids, little studied carotenoids and/orbeneficial actions can be harnessed to increase the competitiveness of the European agro-food industry and promote health? To date over 100 stakeholders from over 30 countries are involved in the Action, which are organized into four working groups (WG), namely 1) Production: developing resources and biosynthetic pathways (WG1); quality along the food chain (WG2); nutrition and health (WG3) and 4) transfer, dissemination and exploitation (WG4). Acknowledgements: This article is based upon work from COST Action EUROCAROTEN, supported by COST (European Cooperation in Science and Technology). T3-11 INVESTIGATING THE CAROTENOID PATHWAY OF LOQUAT FRUIT DURING ONTREE DEVELOPMENTAL STAGES . (adjipieri1, E.C. Georgiadou1,A. Marin2, H.M. Diaz-Mula2, V. Goulas1, V. Fotopoulos1, F.A. Tom|s-Barber|n , G.A. Manganaris 1 Department of Agricultural Sciences, Biotechnology & Food Science, Cyprus University of Technology, Lemesos, Cyprus 2 Department of Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, Murcia, Spain Carotenoids are the main colouring substances found in orange-fleshed loquat fruits. However, relatively few studies focused on carotenoid content during on-tree developmental stages. The aim of the current study was to monitor the carotenoid changes that occur in Obusa fruits, both in the skin and in the flesh. Fruit were harvested from a commercial orchard (Episkopi, Limassol, Cyprus) at ca. 10-day intervals, between the 30th of March until the 14th of May, corresponding to 6 developmental stages. For each developmental stage, 30 representative and free from defects fruits were used for further analysis. Initially, weight, width, length, skin and flesh colour parameters (L*,a*,b*) were monitored. For the carotenoid analysis, fresh samples of both skin and flesh were immediately frozen with liquid nitrogen, powdered, freeze dried and kept at - °C until needed. Fruit weight, length and width ranged between 25.3-59.1 g, 48.8-57.0 mm and 34.4-45.0 mm, respectively. Substantial changes were monitored regarding the colour parameters, both between the flesh and the skin and among the different developmental stages. A significant increment of carotenoid content with the progress of fruit maturation was monitored, both in the skin and in the flesh. In particular, skin carotenoid measurements ranged between 0.80 to 6.12 mg 100 g-1 of fresh weight (FW) and the flesh carotenoid measurements ranged between 0.08 to 5.88 mg 100 g-1 FW, in skin and flesh, respectively, with the highest content being monitored at the last developmental stage. A correlation among colour parameters and carotenoid content was established. Further aim of this project is to dissect key genes and individual 76 III International Symposium on Horticulture in Europe – SHE 2016 compounds that are implicated in the carotenoid biosynthetic pathway with the employment of molecular and advanced analytical protocols (LC-MS). T3-12 N2 FIXING POTENTIAL OF GLIRICIDIA (GLIRICIDIA SEPIUM) WHEN INTERCROPPED WITH COCOA (THEOBROMA CACAO L.) James Seutra Kaba1, Stefan Zerbe1, Akwasi A. Abunyewa2, Massimo Tagliavini1 1 Faculty of Sciences and Technology, Free University of Bolzano/Bozen, Via Sernesi 1, 39100 Bolzano/Bozen, Italy 2 Faculty of Natural Resource, Kwame Nkrumah University , Kwame Nkrumah, Ghana N2-fixing plants in an agro-forestry (intercropping) systems provide an additional source of N for agricultural crops, thus reducing the need for mineral fertilization. This study has been carried out in Ghana (W. Africa), in four cocoa (Theobroma cacao L.) sites, where Gliricidia sepium (Jacq) trees (a N2-fixing species of the Fabaceae family) were intercropped. On average, cocoa trees were spaced 3 x 3 m, while Gliricidia trees were spaced 9 x 9 m. The objective was to estimate the potential of Gliricidia to fix atmospheric N. We used the N natural abundance method, based on the difference in N values between soil-mineral N (assessed using a reference plant, cocoa trees in our study) and atmospheric N2, to assess the N derived from the atmosphere (Ndfa). We also estimated the amount of N in the annual biomass produced by Gliricidia trees. Young, fully expanded leaves of cocoa and Gliricidia were sampled both in the dry and rainy seasons. )n most sites excepting one and in both seasons, N values were significantly lower (at P < 0.05) in Gliricidia than in cocoa. The N concentration in Gliricidia leaves was in the range from 3.2 to 4.1% DW. The Ndfa recovered in Gliricidia leaves ranged from 27 to 65% of total leaf N. The growth estimate showed that Gliricidia is capable of producing about 4.6 t DW ha-1 year-1 aboveground biomass, and that this occurs mainly in the rainy season. In conclusion, we estimated that Gliricidia trees intercropped with cocoa are able to fix from 70 to 82 kg atmospheric N ha-1 year-1 . Therefore, if the growers prune the Gliricidia trees and place the pruned shoots on top of the soil underneath the cocoa trees, the latter will significantly benefit from the Ndfa. T3-13 EPIDEMIOLOGY OF THE ESCA RELATED FUNGUS PHAEOMONIELLA CHLAMYDOSPORA Melanie Molnar1, Ralf Voegele2, Michael Fischer1 Julius K“hn-Institut, Geilweilerhof, 76833 Siebeldingen, Germany 2 University of Hohenheim , Otto-Sander-Strae 5, 70599 Stuttgart, Germany The anamorphic fungus Phaeomoniella chlamydospora (Pch) is associated with grapevine trunk diseases GTDs such as Petri disease or Esca; it was first documented in Mediterranean areas, but can now be found worldwide. GTDs are the most destructive diseases of vineyards worldwide causing high economic losses every year. Typical symptoms can appear on leaves, berries and in the wood. It had originally been assumed that Pch was host specific for Vitis vinifera and Vitis labrusca, but recent reports from California and Italy indicate that also olives, Olea europaea, can be infected. In this case Pch is related to dieback of branches and can be successfully reisolated from cankers. As there are no effective fungicides available, it is even more important to understand the epidemiology of this fungus. To investigate the occurrence and distribution of Pch in the field, spore traps have been placed for three consecutive years in the canopy and at the trunks in vineyards at the JKI in Siebeldingen, Germany. The spore traps were changed weekly and analyzed via nested PCR using specific primers for the ITS region of Pch. First results show that spores of the fungus can be detected throughout the year. A clear correlation between spore detection and weather conditions, for instance rainfall, is not possible, so far. A selection of isolated strains from infected trunks was analyzed using a variety of ISSR-primers. Although the overall genetic diversity of Pch strains proved to be very low, polymorphisms in the patterns were found and specific primers could be developed. These primers were 77 BOOK OF ABSTRACTS used successfully for analyzing DNA from spore traps and it could be shown that more than one genetic group of Pch exists in the vineyards tested. T3-14 ON-TREE TOCOCHROMANOL BIOSYNTHESIS IN `KORONEIKI´ OLIVE FRUIT: AN ANALYTICAL AND TRANSCRIPTOME APPROACH Egli Georgiadou 1, Vlasios Goulas 2, Nikolaos Nikoloudakis 1,Thessaloniki Ntourou 3, George Manganaris 2, Panayiotis Kalaitzis 3, Vasileios Fotopoulos 1 1 Anexartisias 57, Lemesos, Cyprus 2 Anexartisias 33, P.O. Box 50329, 3603 Lemesos, Cyprus 3 Mediterranean Agronomic Institute at Chania, Chania, Greece Vitamin E is a general term used to describe a group of eight fat‐soluble compounds known as tocochromanols. These eight forms of vitamin E are divided into two groups with four forms each, namely ‐, ‐, ‐, and ‐tocopherols and tocotrienols, respectively. The goal of this study was to generate the complete map of the biosynthetic pathway of vitamin E in olive fruit (Olea europaea, cv. Koroneiki through a comprehensive approach that encompasses molecular, biochemical and analytical techniques and included two interrelated parts. Initially, a high resolution temporal mapping of the biosynthetic pathway of vitamin E was performed during 17 successive developmental stages [6-38 weeks after flowering (WAF)] within a single growth season. Results highlighted that olive fruits have increased amounts of all tocopherols and tocotrienol up to 22 WAF (breaker stage) and decreased later on, correlating with the expression profile of VTE5 which is thus proposed as a marker gene for vitamin E levels. The second part of this study focused on the biosynthetic pathway during 8 successive on-tree developmental stages ‐ WAF over three consecutive growth seasons. The key observed motif from the 1st to the 3rd growth season was that the gene expression profile shifted from overall suppression to induction (excluding VTE5), correlating with increasing temperature and decreasing rainfall levels. Furthermore, VTE5 protein levels, tocochromanols content and general antioxidant capacity decreased both across single season developmental stages as well as over the three successive growth seasons. Such differences highlight the impact of environmental conditions, i.e. water levels and air temperature. T3-15 SSR AND ISSR ANALYSIS OF POPULATIONAL VARIABILITY OF LYCHNIS NIVALIS KIT., ENDEMIC AND VULNERABLE ROMANIAN SPECIES Victoria Cristea1, Anca Butiuc-Keul2, Liliana Jarda 1 Babes-Bolyai University, Al. Borza Botanical Garden, Republicii, 42, 400015 Cluj, Romania 2 Babes-Bolyai University, M. Kogalniceanu,1, 400048 Cluj Cluj-Napoca, Romania 3 Baladei, 35, Cluj Cluj-Napoca, Romania Molecular techniques are very important in studying the conservation of biodiversity. Molecular markers are very useful tools for estimation of genetic polymorphism in the populations. In order to develop a proper preservation program for vulnerable, rare or endemic species, it is very important to study and to preserve the natural genetic polymorphism. Lychnis nivalis Kit. (syn. Polyschemone nivalis Schott, Nym. et Kotschy, syn. Silene nivalis Rohrb.) is endemic in Rodnei Mountains. It is considered rare or vulnerable in Romanian Red Book and in the Romanian Red Lists. The taxonomic status of this species is controversial. This species have ornamental values due to the caespitose appearance and the purple or white flowers with two-lobed petals. The genetic variability analysis was used in order to assess the in situ conservation status of this species, by studying 10-20 individuals from 5 different populations (near Iezerul Lake, Lala Mare Lake, Stiol Lake, Inau Peak, Galati Mountain). The appropriate out-group species was Silene acaulis (L.) Jacq. (Fagaras Mountains). Molecular markers as SSR and ISSR were used to investigate the genetic variability in the populations of L. nivalis. SSR markers generated with MS-DCDIA30, MS-DINCARACC and MS-DINMADSBOX showed no polymorphism. The primers MS-DCAMCRBSY and MS-DINGSTA showed low level of 78 III International Symposium on Horticulture in Europe – SHE 2016 polymorphism especially in the populations from Lala Mica, Galati and Stiol. ISSR markers also showed low genetic polymorphism in most of the populations. The markers generated with BC864 primer showed genetic polymorphism in populations from Lala Mica and Stiol, while the markers generated with BC880 primers showed genetic polymorphism in Inau and Galati populations. T3-16 DIFFERENT EXPRESSION OF HELICASE GENES IN ARTIFICALLY AND NATURALLY INDUCED MANGO FRUITLET ABSCISSION Patrick Winterhagen, Michael (. (agemann, Jens N. W“nsche University of Hohenheim, Agriculture, Emil-Wolff-Str. 25, 70599 Stuttgart-Hohenheim, Germany Premature fruit drop is a severe production constraint for mango (Mangifera indica) and recent research demonstrates that various physiological parameters and genes are involved in the abscission process. In addition to hormone and carbohydrate responses in the fruitlet, it is evident that ethylene receptors are involved in abscission zone activation. The mango ethylene receptors MiERS1 and MiETR1 respond differently to abscission-inducing ethephon treatments and their expression pattern indicates plant organ specificity. Furthermore, two putative ATP dependent RNA helicases were identified on the genomic sequence located upstream of the MiERS1 gene. RNA helicases are important for RNA metabolism on the level of gene expression or post-transcription. One of the identified helicases was found to be upregulated in the pedicel abscission zone after ethephon treatment. In contrast, both identified helicases were downregulated in pedicels of fruitlets naturally induced for abscission. When ethephon-induced fruitlet abscission is compared to naturally induced abscission there are certain differences in the helicase gene expression pattern, as well as in the ethylene receptor expression pattern, while other physiological responses appear to be similar. The expression pattern of the helicases suggest that they might play a role in the fruit abscission process, however, they may not or only partly be inducible by ethylene, suggesting that they act upstream of ethylene signalling. T3-17 BIOTECHNOLOGICAL APPROACH TO CONTROL PPV RESISTANCE IN PLUM Tatiana Sidorova 1, Roman Mikhailov 2, Tatiana Vagapova 2, Alexander Pushin 2, Sergey Dolgov 2 1 Institute of Bioorganic Chemistry, Nikita Botanical Gardens, Russian Federation 2 Science Ave 6, Pushchino, Russian Federation Plum pox virus (PPV) is the serious viral disease affecting Prunus species such as plum, apricot, cherry and peach. To date the few PPV resistance genetic resources found in Prunus germplasm, nevertheless the conventional breeding approaches are very challenging for use in fruit trees due to several limiting intrinsic factors. Several biotechnological approaches could be used to develop PPV resistance in plants; nonetheless the RNA interference is shown to be the most effective disease-control strategy. Last decade RNAi-mediated virus-resistant plants of commercial cultivar Startovaya were produced in our laboratory. pCamPPVRNAi vector containing the self-complementary sequences of fragment of PPV-CP gene separated by an intron to produce a hairpin RNA structure was created for transgenic plum generation. Transgenic trees were inoculated by grafting with PPV-M strain infected buds, which formed branches that were constant source of PPV. The genetically modified plum threes has proven to be highly resistant to PPV for five years. In fact, the expectation that the RNA silencing signal moving from the transgenic scion to non-transgenic rootstock would be able to confer PPV resistance was not justified. For this reason the production of PPV resistant rootstock, besides the virus resistant cultivar, is highly recommended to obtain completely virus resistant mature plum trees. To date, there have not been reports of successful production of commercial plum rootstock resistant to PPV. Here we present the development of an agrobacterium-mediated protocol for transformation of commercial plum rootstock Elita-37-27Prunus domestica L. using leaf explants. 79 BOOK OF ABSTRACTS The system enables the efficient plant regeneration with retention of the original rootstock traits. )ndependent putative transgenic plum plants with fragments of hairpin -PPV-CP gene have been produced. We expect that robust and predictable PPV resistance will be achieved in mature trees in the case of combination of transgenic rootstock and commercial variety. T3-18 GENETIC BASIS AND ENVIRONMENTAL FACTORS PLAY ROLE IN CYSTEINE SULPHOXIDES CONTENT OF GARLIC CULTIVARS Jaroslava Ovesna 1, Katarina Mitrov| 1, Pavel Svoboda 1, Vojtech Hrbek 2, Jana (ajslov| 2 1 Crop Research Institute,Drnovska 507, 16106 Prague 6, Czech Republic 2 ICHT- Technick| , Prague 6, Czech Republic Garlic (Allium sativum L.) is a precious vegetable that have been consumed for millenia as a culinary and medical species. Garlic belongs to Alliaceae family and is known to have a large genome. Garlic have been vegetativelly propagated species as during history lost its capacity to flower and produce true seeds. Two basic morphotypes are known: bolting types known as A. sativum ssp. ophiscorodon that may preserved some potential to flower and non bolting A. sativum ssp. sativum that do not produced scapes. Cloves of genotypes registered as cultivars are used for garlic production. Breeding of new cultivars rest on selection of best performing progenies of availble clones. Apart yield, content of health promoting compouds is another required feature of garlic. They are represented mainly by cysteine sulfoxides (SACSs). Consumers from different regions require various garlic pungency that is mainly associated with mentioned cysteine sulfoxides. Recently we showed association between genetic background of individual clones representing precious genetic resources as assessed by SSR and AFLP makers. Content of SACSs ranged from 4.2 mg/g of SACSs up to 18.6 mg/g SACSs. That means still certain variability exists within genetic resources that allow for selection of new cultivars. Garlic is a demanded vegetable in the Czech Republic with described history dated back to 6th century. Local consumers require local cultivars with strong taste, preferably bolting ones. Genetic analysis showed that Czech bolting cultivars associated together regardless breeding provenience. Other European or Argentenean bolting cultivars associated together with Czech non bolting garlics. The results indicate specific genetic background of Czech bolting garlic cultivars. Apart total SACSs content ratio between individual SACSs types differ from garlics of other origin. Metabolomic analysis showed specific feature of Czech bolting garlics, even the profiles depends on environmental and cultivation conditions as it was shown over three years and several localities. To investigate impact of environmental conditions a RTqPCR assay was validated to assess transcription of genes involved in SACSs biosynthesis. We demonstrated that the assay allows for investigation of enviromnetal factors upon SACSs production. The work was suported by projet of National Agency for Agricutural Research (Ministry of Agricuture) project No. QJ1210158 and RO014 T3-19 MOLECULAR BACKGROUND OF THE FLAVANONE 4-REDUCTASE ACTIVITY OF DIHYDROFLAVONOL 4-REDUCTASES Christian Gosch, Lukas Eidenberger, Christopher Schlosser, Phillip Waldhör, Silvija Miosic, Karl Stich, Heidi Halbwirth Vienna University of Technology, Institute of Chemical Engineering, Getreidemarkt 9, 1060 Wien, Austria Dihydroflavonol 4-reductase (DFR) is a key enzyme of the flavonoid pathway catalyzing the formation of precursors for the biosynthesis of anthocyanin pigments and monomeric flavan 3-ols, the building blocks of tannins. Although dihydroflavonols are the main substrates, flavanones can be converted as well, leading to the formation of 3-deoxyflavonoids. The latter reaction is known as flavanone 4-reductase (FNR) activity. The two flavonoid classes differ by a hydroxyl group in position 3 of the heterocyclic ring. 80 III International Symposium on Horticulture in Europe – SHE 2016 The majority of DFRs show a strong dihydroflavonol preference but we identified two further DFR types which either accept exclusively dihydroflavonols or both substrates to a similar extent. Sequence comparison of the three DFR types and site directed mutagenesis revealed only few significant differences in the amino acid sequence around the presumed substrate recognition sites, which are essential for the coordination of the hydroxyl group in position 3 and therefore are decisive for the ability/inability to convert dihydroflavonols and flavanones, respectively. In-depth understanding of the molecular background of the 3-deoxyflavonoid formation is an important contribution for the elucidation of the biosynthesis and physiology of 3-deoxyanthocyanidin phytoalexins and pigments, as well as for protein engineering in general. T3-20 ETHYLENE RECEPTORS PROTECT THE EMBRYO OF DEVELOPING APPLE FRUITLETS FROM THE ABSCISSION SIGNAL: AN IMMUNE-LIKE MECHANISM? Giulia Eccher1, Maura Begheldo1, Andrea Boschetti2, Benedetto Ruperti1, Alessandro Botton1 DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly 2 Nanoscience Research Unit, FBK, 38123 Trento, Italy Apple (Malus x domestica) is increasingly being considered a model species for studies related to fruit development, in particular for the earliest stages, during which an extremely relevant phenomenon, i.e. fruitlet abscission, may occur as a response to a complex series of endogenous and exogenous cues. Several studies were carried out shedding light on the main physiological and molecular events that lead to the selective release of lateral fruitlets within the apple corymb, either occurring naturally or as a result of a thinning treatment. Several studies evidenced a clear correlation between the rise of ethylene biosynthesis in the fruitlet and its tendency to abscise. A direct mechanistic link, however, has not yet been established between this gaseous hormone and the generation of the abscission signal within the fruit. In the present study, the role of ethylene during the very early stages of abscission induction was investigated in fruitlet populations with different abscission potentials due either to the natural correlative inhibitions determining the so called physiological fruit drop or to a well tested thinning treatment performed with the cytokinin benzyladenine. A crucial role was ascribed to the ratio between the ethylene produced by the cortex and expression of ethylene receptor genes in the seed. This ratio would determine the final probability of the fruitlet to abscise. A working model is proposed consistently with the differential localization of four receptor transcripts within the seed, which resembles a spatially progressive cell-specific immune-like mechanism evolved by apple to protect the embryo from harmful ethylene. 81 BOOK OF ABSTRACTS Oral Topic – 4 Tools, Technologies, Innovations and Applications 82 III International Symposium on Horticulture in Europe – SHE 2016 T4-O1 IMPLEMENTATION OF PHOTOVOLTAICS ON GREENHOUSES AND SHADING EFFECT TO PLANT GROWTH Aggeliki Kavga 1, Georgios Trypanagnostopoulos 2, Athanasios Koulopoulos 1, Yiannis Tripanagnostopoulos 3 1 Dept. of Agricultural Technology, T.E.I., Amaliada, Greece 2 Mech and Aeron Engineering Univ of Patras, Patra, Greece 3 University of Patras, Physics Department, Patra 26500, Greece Greenhouses are sophisticated structures, which require optimum combination of solar radiation, heating, cooling and ventilation to produce a good/high quality crop within a predictable and repeatable time schedule. Energy demand of greenhouses is an important factor for their economics and photovoltaics (PV) can be considered an alternative solution to cover their electrical and heating needs. The installation of PV panels on the greenhouse s roof reduces solar radiation that passes through roof glazing and falls to the plants into the greenhouse. This reduction may affect the plant lighting and it should be studied regarding the optimal installation to minimize the reduction of solar radiation that enters the greenhouse interior space. In this paper, a PV system properly mounted to greenhouse roofs has been studied experimentally in two identical, small scale experimental greenhouses. The two greenhouse options were implemented with and without PV correspondingly. Lettuce, eruca sativa and strawberry are used as the test crops for a six months period. The design and energy performance of the above PV installation modes is analysed and results are presented. An analysis of plants nutrients absorption is provided, which reveals the effect of shading in the PV greenhouse plants. T4-O2 LONG-TERM PREDICTION OF BIOSIGNALS FROM GREENHOUSE-GROWN TOMATO Luis Miranda1, Bruno Lara2, Thorsten Rocksch1, Dennis Dannehl1, Uwe Schmidt1 1 Humboldt-Universität zu Berlin, Biosystems Engineering Group, Albrecht-Thaer-Weg 3, 14195 Berlin-Dahlem, Germany Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico Artificial Neural Networks (ANN) have been succesfully used for modelling climate and plant signals in greenhouses. In this work, we evaluated the combination of two ANN models, built and trained separately, and then coupled together. Both models were trained using a large dataset consisting on 3 complete cultivation periods (2011 through 2013) of 2 venlo-type greenhouses located at the Humboldt-Universität zu Berlin. The greenhouses were used for soilless cultivation of tomato Solanum lycopersicum Pannovy , Komett and Encore . The first model considered (ANN_1) estimated the expected values of temperature and relative humidity in the near future (One-Step Prediction, OSP). The output was then recursively fed to the network in order to make further predictions. Each time step represented 5 minutes, and the maximum number of prediction steps was set to 6. On the other hand, the output of the second model (ANN_2) was not a prediction in time, but an estimation of plant signals (leaf temperature, transpiration rate, photosynthesis rate) as expected to be measured by a phytomonitoring system (BERMONIS). The combined system shown here took the 6 predicted steps of climate (Long-Term Prediction: LTP) produced by ANN_1 and used them to feed ANN_2, thus generating a Biosignals LTP. As expected, the fit of the LTP decreased with the number of steps predicted. For example, the R for Leaf Temperature range from 0.949 for the first simulation until 0.779 for the 6th simulated step. For Transpiration and Photosynthesis Rate, these ranges were [0.891,0.810] and [0.787,0.735], respectively. These results show that the estimated plant responses could support the construction of a predictive control system, in order to avoid plant damage due to extreme climate conditions. 83 BOOK OF ABSTRACTS T4-O3 BEYOND RAIN PROTECTION: FRUITS PLASTIC PROTECTION SHEET AS LIGHT SPECTRUM TRANSMISSION INFLUENCE MEANS" Massimo Bizzarri Oroplus® for Agricultural Division BEYOND RAIN PROTECTION research program is orientating the fruit protection sheets towards diseases prevention, nutraceutical characteristics enhancing, crops climatic conditions influences. Considering the fruits protection sheet only as rain protection, as the traditional point of view is still considering, imply to let drop the most interesting opportunities offered by this application. The results of the already year s research program, analysed in the presentation from the unusual point of view of a company commercial responsible, like the author is, strongly underline the need of a mandatory collaboration between research and production entities. Improve the global efficiency of a elementary production means, as a plastic protection sheet apparently is, is the main goal obtained by this research program and, in addition, also by linking professional roles that, at first sight, could be considered oxymoronic. T4-O4 DEVELOPMENT OF AN OPTIMAL MODEL-BASED ION-SPECIFIC NUTRIENT CONTROL OF HORTICULTURAL CROPS Thorsten Rocksch 1 , Uwe Schmidt 1, Hans-Peter Kläring 1 Humboldt-Universität zu Berlin, Division Biosystem Engineering, Albrecht-Thaer-Weg 3, 14195 Berlin, Germany 2 Leibniz Institute of Vegetable and , Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Grossbeeren, Germany Closed fertilization systems improve the water use efficiency of greenhouse crop production considerably thereby avoiding pollution of the ground water. However, the uptake ratio of water and the single nutrients is not constant over the cultivation period of most crops. This may result in ion imbalances in the nutrient solution, if the composition of supply solution is not adequately adapted to the growth stage of the crop and the growing conditions. For perfect adjustment an ion-specific nutrition control will be developed and tested. The optimal composition of the supply solution will be estimated based on models of the plant s water and nutrient uptake depending on the stage of the phenological development and the environmental conditions. Ion-specific sensors measure on-line the composition of the drainage solution. First, the composition of the single nutrients in the solution was not changed but the total concentration of nutrients expressed as electrical conductivity (EC) was varied depending on the climate in the greenhouse. The corresponding strategy was derived from models of photosynthesis and transpiration. The set point of the concentration of the nutrient solution was adjusted daily in the morning depending on the vapor pressure deficit expected for the day. This strategy was compared with the supply of nutrient solution of a constant EC of 3 dS m- as often used in horticultural practice. Tomato cv. Pannovy was grown in rockwool slabs in a greenhouse with closed nutrient cycles at Großbeeren °N from April to September , . )onspecific sensors measured on-line the composition of the drainage solution. Additionally, laboratory measurements were carried out fortnightly to determine the nutrient composition in the supply and drainage solution. Climate-driven adjustment of the EC in the nutrient solution resulted in more stable and on average lower EC in the drainage solution compared to supply of solution at a constant EC of 3.0 dS m-1. During a harvest period of 12 weeks, the solution with variable EC provided a significantly higher total yield compared to a constant EC and less fruits were infested with blossom end rot. 84 III International Symposium on Horticulture in Europe – SHE 2016 T4-O5 PROCESS-BASED SIMULATION MODELS AND PLANT PHENOTYPING Michel Génard, Daniele Bevacqua, Francoise Lescourret INRA, UR 1115 PSH, Domaine Saint-Paul, Site Agroparc, CS 40 509, 84914 Avignon Cedex 9, France Plant phenotyping is difficult due to the complexity of the plant system and its sensitivity to developmental stages, environmental conditions and interactions between these and the genotype. Process-based simulation models can combine different biological functional hypotheses on responses of plant processes to environmental fluctuations. They then allow a virtual representation of plant functioning and can be useful tools to phenotype plants in terms of ecophysiological processes. The Virtual Fruit model developed by adapting and connecting existing models describing fruit growth, transpiration and respiration, sugar and acid accumulation and ethylene production was used to illustrate the approach. It permitted to compare and contrast performance of a virtual wild-type plant and a virtual mutant, and then analyse the impact of a single mutation on different processes in case of decrease in leaf number due to pest injury (18 versus 6 leaf/fruit). The tested virtual mutations corresponded to (1) an increase by a factor 5 of the mobile fraction of plant reserves that can be mobilised to sustain carbon supply to the fruit or (2) a decrease by 70% the fruit s requirement for carbon. The defoliation induced by pest and the mutation on fruit s requirement for carbon had large effects on several physiological processes (growth, respiration, metabolism and maturation) and quality traits (size, sucrose and citric acid concentration). In contrast, the mutation on reserve mobilization had only a weak effect on fruit growth and acidity. Such a virtual profiling approach can open new avenues for exploring in silico the impact of genetic variations or pest damage. T4-06 PHENOLOGY AND FRUIT SET OF PROMISING NORTH AMERICAN SHRUBS IN THE GENUS DIRCA William Graves 1 Iowa State University, Department of Horticulture, Ames IA, 50011-1100, United States of America The genus Dirca (Thymelaeaceae) comprises four species of deciduous shrubs noted for their unusual time of flowering (autumn through early spring), yellow autumnal leaf color, and arborescent growth habit. Despite their attractiveness, these species rarely are marketed as horticultural plants due to their scarcity in nature and to difficulty with producing them in nurseries. Propagation from seed is possible, but fruit set (the percentage of flowers that lead to a fruit) of Dirca reportedly can be poor. My objective was to quantify fruit set of Dirca occidentalis at multiple sites within its native distribution in a small portion of California with a Mediterranean climate. Comparisons were made to Dirca palustris in the eastern United States, and the phenology of plants of both species was tracked as they broke dormancy to flower and form new stems and leaves. Data collected over five consecutive years showed variation in fruit set was more highly associated with the timing of individual flower formation than by site in the landscape. Fruit set ranged from 0% to 38% depending on site and time of flowering. In a separate study, individual plants of both species differed markedly in fruit set, and phenological state within populations was more variable among plants of D. occidentalis than plants of D. palustris. Environmental data support inferences about the importance of annual variation in air temperature and precipitation. Fruit set was promoted by warmth, perhaps due to its effect on insect pollinators, and precipitation was positively correlated with early anthesis. These data are being used in a comprehensive assessment of the potential value of Dirca species for use in ornamental horticulture. 85 BOOK OF ABSTRACTS T4-07 INAPRO - INNOVATIVE AQUAPONICS FOR PROFESSIONAL APPLICATION Johanna Suhl1, Baganz Daniela2, Werner Kloas2, Dennis Dannehl1, Sebastian Jobs3, G“nther Scheibe , Uwe Schmidt1 1 Humboldt-Universität zu Berlin, Thaer-Institute, Biosystem Engineering, Albrecht-Thaer-Weg 3, 14195 Berlin, Germany 2 Leibniz-)nstitute of Freshwaer Ecology and, )nland Fisheries, M“ggelseedamm , Berlin, Germany 3 PAL-Anlagenbau GmbH, Amtsweg 6, 18510 Abtshagen, Germany In contrast to conventional single recirculation aquaponic systems (SRAPS) the unique and innovative INAPRO double recirculation aquaponic system (DRAPS) is enhanced by the combination of two separate recirculating systems: the recirculating aquaculture system (RAS) for fish rearing and a closed hydroponic cycle for plant production. Both systems are connected in one direction to transfer the nutrient rich fish waste water to the hydroponic unit. Before the fish water is applied to the plants, it is optimized for plant growth by addition of mineral fertilizer. A combination of tilapia (Oreochromis niloticus) and tomatoes (Solanum lycopersicum) were chosen for the first experiments in the INAPRO research prototype, started in January 2015. The tomatoes grew in nutrient film technique (NFT) and three different treatments were applied: control (conventional nutrient solution, electric conductivity (EC) 1.8); fish water (FW) plus mineral fertilizer (aquaponics, EC 1.8); FW plus mineral fertilizer (aquaponics, EC 3.0). The results showed that the total leaf area growth of plants and the number of leaves per plant produced in aquaponics, EC 1.8 was significant reduced compared to that in the other both treatments. No differences in the number of trusses, fruit setting and plant length were observed. The total yield harvested in aquaponics, EC 1.8 was similar to that in the control. This means that with the innovative DRAPS same yields can be realized like in commercial hydroponic production. Furthermore, the fertilizer use efficiency (kg tomato yield per kg total fertilizer supply) was significantly improved by 23.6% caused by aquaponics, EC 1.8 compared to the control. Additionally, fresh water could be saved by using fish waste water. In aquaponics, EC . . kg tomatoes and . kg fish were produced with one m fresh water, whereas in the control just 47.7 kg m-3 tomatoes were produced. T4-O8 OPTIMIZATION OF CULTURAL PRACTICES IN A MULTI-OBJECTIVE FRAMEWORK VIA A SIMPLE MECHANISTIC MODEL FOR CARBON ASSIMILATION AND ALLOCATION IN FRUIT TREES Daniele Bevacqua 1, Paco Meli{ , Francoise Lescourret 3, Michel Génard 3 , Renato Casagrandi 2 , 1 )NRA, Route De L'aÃ©rodrome. CS 40509, Domaine St Paul. Site A, 84914 Avignon, France 2 45 via ponzio, milano, Italy 3 Site Agroparc, 84140Avignon, France We propose a simple mechanistic model describing carbon assimilation and allocation to shoots, roots and fruits in temperate fruit trees, explicitly accounting for the effects of cultural practices (i.e. winter pruning and fruit thinning). We calibrated it against experimental data on peach trees Prunus persica, subject to different intensities of pruning and fruit loads, and used it to simulate consequences of multiple possible combinations of pruning and thinning intensities. Eventually we derived optimal combinations of practices with respect of different objectives such as maximizing short and long term revenues, by using the concept of Pareto boundary.The model well reproduced observed variations in overall fruit yield and shoot production, single fruit and shoot size in different trees over the vegetative period; and provided insights into reserve accumulation and root growth. Results suggest that shoots and fruit growth in non-pruned and non-thinned trees is limited by assimilates supply thus resulting in shorter shoots and smaller fruits with little economical value. On the other hand, organs growth is demand limited in case of pruning and thinning thus resulting in reduced overall yield, yet composed by bigger fruits. Assimilates reserve at the end of the vegetative season 86 III International Symposium on Horticulture in Europe – SHE 2016 is enhanced by thinning yet decreases with intense pruning. When aiming to maximize short and long term revenues, optimal practices consist in decrease fruit load by leaving ca. 5 fruits per meter, while pruning intensity can vary between 0 to 60% with higher values favoring long, rather than short term, revenues. T4-O9 HISTORICAL PHENOLOGICAL RESPONSES IN APPLE TO CONTRASTING WARMING CONTEXTS MAY CLARIFY FUTURE CRUCIAL RESPONSES IN EUROPE Jean-Michel Legave 1, Isabelle Farrera 2, Adnane El Yaacoubi 3, Gustavo Malagi 4, 1 Cirad av. Agropolis - TA A-9603, 34398 Montpellier, France 2 Montpellier SupAgro, UMR AGAP, Cirad, Av. Agropolis,TA-A-10803, 34398 Montpellier, France 3 Université Moulay )smail, Meknès, Morocco 4 Faculdade de Agronomia, Universidade Federal de Pelotas, Pelotas-Rio Drande do Sul, Brazil Phenological responses to warming in temperate fruit trees have rarely been investigated in contrasting warming contexts. This framework is appropriate for highlighting varying responses that would combine chill accumulation declines and heat accumulation increases. A comprehensive overview of historical responses might clarify crucial future responses in the warmest European regions. To examine this issue, a dataset was constituted in apple from ﬂowering dates collected for main BBC( stages in contrasting temperate regions of Western Europe and in different mild regions (Northern Morocco, Southern Brazil). Multiple change-point models were applied to series of ﬂowering date, flowering duration and temperature, aiming to statistically analyse both ﬂowering responses and temperature changes. Modelling of flowering date was used to understand the determinisms of warming responses. In addition, regional differences in dormancy dynamic were characterized by forcing tests. Statistical analysis provided an overview of ﬂowering date responses at global scale, highlighting ﬂowering advances, mainly in Europe and Morocco, but also stationary ﬂowering date series in Brazilian and French Mediterranean regions. Modelling analysis supported the notion that flowering advances are due to heat accumulation increases, explained by marked warming during ecodormancy. Later dormancy releases due to chill declines were also supported in Europe and may explain, in long term, stationary flowering dates in the French Mediterranean region. While the ﬂowering duration series were stationary whatever the region, the ﬂowering duration was far longer in Moroccan and Brazilian regions compared to European. This was linked to contrasting differences in dormancy dynamic observed between these mild and temperate regions. Dormancy release was inadequate in mild due to poor chill accumulation, leading to extended flowering durations. Since later dormancy releases would already have significant impacts on flowering date in Europe, new crucial warming responses, such as extended flowering durations, could be experienced especially in Mediterranean regions in near future. T4-10 MODELLING FRUIT PLANT-PEST INTERACTIONS, THEIR CONTROL BY CULTIVATION PRACTICES AND THEIR LINK TO FRUIT QUALITY Françoise Lescourret 1, Caroline Gibert 2, Isabelle Grechi 3, Michel Génard 4, Daniele Bevacqua 4 1 INRA UR1115 Plants and cropping Systems in , Horticulture, Domaine Saint-Paul, Site Agroparc, 84914 Avignon Cedex 9, France 2 Solagro, 75 voie du TOEC, CS 27608, 31076 Toulouse, France 3 CIRAD, UPR HortSys, Station Bassin Plat, BP 180, 97455 Saint Pierre, France 4 INRA, UR1115 PSH, Domaine Saint Paul , site Agroparc, 84914 Avignon cedex 9, France Plant-pest interactions are extensively investigated at the molecular level but much less at organ or whole plant levels with a modelling approach. At these levels, in the case of cultivated plants, models are useful tools not only to understand the processes at play but also to study their control by cultivation practices. We present a general framework of fruit plant-pest interactions and we use two case studies, peach-brown rot and peach-green aphid, to demonstrate the interest of diverse models to address contrasted pathosystems. 87 BOOK OF ABSTRACTS First, we exemplify the need of gaining insight into the sensitivity of the host to the pest, because this sensitivity may take a large variety of forms – cuticular cracking for peach-brown rot, quality of the foliage for peach- green aphid. It is also necessary to understand how cultivation practices such as irrigation, thinning or pruning may control this sensitivity. Second, we exemplify modelling choices adapted to the pathosystem. For example, a dynamic coupling of plant growth and pest demography is adapted to peachaphid but meaningless for peach-brown rot. Third, we show that relating plant-pest interaction to fruit quality involves different key variables according to the pathosystem. Fruit surface conductance and tree foliage are such key variables in peach-brown rot and peach-aphid, respectively. Finally, we present simulations of scenarios that exemplify the ability of the modelling approach to relate the management of plant-pest interactions to fruit quality. T4-11 TEMPERATURE-SUM MODELS FOR IRRIGATION SCHEDULING OF VEGETABLE CROPS IN OPEN FIELD Jana Zinkernagel, Matthias Olberz Geisenheim University, Department of Vegetable Crops, Von-Lade-Str.1, 65366 Geisenheim, Germany Irrigation efficiency is a key challenge for vegetable production regarding availability and sustainability of water resources. In contrast to the multitude of irrigation scheduling systems (ISS), limited practicability is likely to be a major constraint for their use. The Geisenheim Irrigation Scheduling (GS), similar to FAO-56 single kc procedure, is suited for vegetable crops grown under Central European climate conditions. Its calculation procedure requires the determination of plant growth stages and the use of single crop coefficients (kc), which change stepwise with growth stages. This ISS must be improved regarding userfriendliness. This study proves whether kc steps may be replaced with continuous kc, modelled with temperature-sums (TS). Using modelled kc, the recording of plant growth stages in course of crop development might be obsolete. With lettuce (Lactuca sativa L. var. crispa cv. Linaro, Rijk Zwaan, NL) as model crop, field trials were carried out from 2013 to 2015 in Geisenheim, Germany. TS-models of crop growth were parametrized by weekly determined fresh weight (FW), dry weight, leaf area and leaf number. Growth rate was related to TS considering base temperature of °C. Derived curve parameters were used to establish kc curves with kcmin and kcmax of GS. This parametrization was evaluated against water supply and yield in field experiments in 2015 with three irrigation treatments: kc-TS model, GS and FAO-56 dual kc procedure. The best model fit was found for FW described with a saturation curve. Derived parameters were implemented in the kc-TS model: kcTS = kcmax − kcmin / + b ∗ exp(−c ∗ TS)) + kcmin with b = 208.1 (p = 0.07) and c = -0.01294 (p = 7.8e-05). The model evaluation resulted in reduced water supply without significantly reduced FW, when compared with GS and FAO-56. Thus, modeling kc via TS may improve userfriendliness of ISS. T4-12 TOWARDS ZERO DISCHARGE IN HYDROPONIC CULTIVATION Jim Van Ruijven1, Erik van Os2, Ellen Beerling1, Jan Janse1, Marieke van der Staaij1 1 Wageningen UR Greenhouse Horticulture, Postbus 20, 2665 ZG Bleiswijk, Netherlands 2 Postbus 644, 6700 AP, Wageningen, Netherlands Competition for freshwater resources, and legal restrictions on discharge of agrochemicals (plant protection products and fertilisers) increase pressure on agricultural practice to improve water-use-efficiency of crop production globally. Zero-discharge greenhouse crop production can be an effective solution to both proble Preliminary results of experiments with cucumber and sweet pepper on stonewool substrate in two greenhouse compartments showed that productivity did not significantly differ in zero-discharge growing 88 III International Symposium on Horticulture in Europe – SHE 2016 compared to standard growing strategies. In this paper we present the final evaluation of the sweet pepper trial (December 2014 – November 2015). In the reference compartment a total amount of 430m3 ha-1 y-1 . kg N was discharged opening the slabs, . kg N; first months drain, 21.1kg N; filter rinsing and refreshment of nutrient solution because of sodium build-up, 139.8kg N). In the zero-discharge compartment there was no discharge during cropping. The nutrient composition of irrigation and drain water was analysed weekly (compared to two-weekly in reference) and nutrient stock composition was changed accordingly. Drain water was filtered with a flatbed filter (no rinsing water) and upon changes in fertigation, the new fertigation solution was first distributed throughout the entire irrigation system by a circulation pump, before application. An irrigation strategy was developed for the end of the cropping season, aiming to drain substrate slabs and to empty drain tanks as much as possible and reduce the amount of remaining nutrients in the slabs, without compromising productivity. All water used for cleaning the greenhouse at the crop rotation was stored and treated with ozone to be used in the next production cycle. In the zerodischarge compartment water use was 430m3 ha-1 y-1 lower than in the reference (5% of supply), whilst both yield and quality were similar for both compartments. T4-13 NON-DESTRUCTIVE DETECTION OF THE OPTICAL PROPERTIES OF THE WAX BLOOM OF PLUMS Michael Blanke 1, Daniel Zemke 2, Lutz Damerow 3 1 Institut fuer Obstbau -INRES Hort Science, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany 2 INRES-Hort.Science, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany 3 Institute of Ag Engineering, University of Bonn, Nussallee 5, D-53115 Bonn, Germany On maturation, plums develop a pronounced layer of epicuticular wax (scenting) that affects its visual appearance. During harvest, packaging and transport, this protective layer may be damaged or removed. The resulting appearance generates the impression of poor fruit quality. The aim of this research was to analyse and compare the influence of this wax layer on the optical reflection using three modified surface features. Weight loss was also recorded of plums with the natural wax layer, polished by hand or wax removed chemically with chloroform and stored for one week. European plums Prunus domestica L. cv. Topper , Topened , Presenta , (auszwetschke Wollf , yellow mirabelle and greengage were carefully picked at the University Experimental station or freshly purchased from market without disturbing the wax surface. Light reflection was measured perpendicular to the plum equator with a dedicated Keyence sensor type Luster Detection Sensor CZ-H72 and doubled from 150- 250 (arbitrary units) to 300-600 (arbitrary units) after polishing the plums. Light reflection of European Plums were decreased in case of the polished surface, while with wax natural layer results were seen more uniform. This case study explains the short shelf life and effects of water loss of the plums under different surface conditions with wax, polished and chemically treated. The sensor technology technique used in this research may be used further for sorting and grading purposes. T4-14 DEVELOPMENT OF SWEET CHERRY (PRUNUS AVIUM L.) AND APRICOT (PRUNUS ARMENICA L.) VARIETIES AND PRODUCTION SYSTEMS IN CALIFORNIA AND EUROPE Stephen Southwick OG Packing Co.-SMS Unlimited LLC, 2097 Beyer Lane, Stockton, California 95215, United States of America There is an ongoing need for sweet cherries and apricots that are improved over those existing. Improved and regular production, fruit size, firmness, time of maturity, packing, shipping and organoleptic charactristics are some traits that can be improved within the varietal maturity window. Over the last several years, we have released (4 patented in Europe and others pending in the world) several sweet cherry varieties with early fruit ripening characteristics that are productive with large fruit size over a range of 89 BOOK OF ABSTRACTS geographic locations with commerical potential. A number of apricots have been patented in Europe and the world and commercialized. We have identified pollination and production strategies toward maximizing the potential of these varietial selections. The development of these varieties and production strategies in Europe and worldwide will be discussed and reviewed as will future developments. T4-15 EVALUATION AND COMPARISON OF A NEW TYPE OF TEMPORARY IMMERSION SYSTEM (TIS) BIOREACTORS FOR MYRTLE (MYRTUS COMMUNIS L.) Ozhan Simsek1, Belgin Bicen1, Dicle Donmez2, Yildiz Aka Kacar1 1 Cukurova University Agriculture Faculty, Horticulture Department, 01330 Adana, Turkey 2 Cukurova University, Institute of Natural and Applied Sciences, Biotechnology Department, 01330 Adana, Turkey Myrtle, Myrtus communis L., is a diploid (2n = 2x = 22) species native to the southern Europe and North Africa. This species is used as an ornamental in areas with Mediterranean-type climates, and it is also valued as a source of secondary metabolites. In the present study we investigated the effects of Plantform Temporary Immersion Bioreactor System for in vitro micropropagation and rooting in Myrtus communis L. and evaluated this new bioreactor for micropropagation using the important horticultural species Myrtle. Shoot tips of M. communis were cultured and propagated on MS medium supplemented with 1 mg L-1 BAP. Shoots were transferred to Plantform vessels and agar medium containing 1 mg L-1 BAP. Plants were subcultured three times in every six weeks for both syste Multiplication rate, plant length, leaf number, dry and fresh weight of plants were recorded and compared for both syste TIS and agar medium were used for also rooting of plant samples. Shoots coming from micropropagation were rooted on MS medium supplemented with 1 mg L-1 IBA. After six weeks of rooting culture number of root, root length, plant length, dry and fresh weight were determined and compared for TIS and agar medium. Rooted with well-developed shoots and roots were removed from magenta and TIS boxes and the roots were washed gently under running tap water and dipped in a solution containing fungicide for 10 s then transferred to plastic pots containing autoclaved peat and perlite (1:1, v/v). The potted plants were placed in a greenhouse under natural light. Acclimatized plants were compared after eight weeks. Based on the comparison of TIS and agar media for Myrtle micropropagation high positive effects were determined. TIS presented here showed good potential for application in large-scale propagation. T4-16 DIGITAL PICTURE ANALYSIS FOR PRECISION SCREENING OF SOUR CHERRY PROGENY FOR NECROTIC LEAF TISSUE CAUSED BY BLUMERIELLA JAAPII Martin Jensen Department of Food Science, Faculty of Science and Technology, Aarhus University, 5792 Aarslev, Denmark Sour cherry generally holds limited resistance to attack by cherry leaf spot fungus (Blumeriella jaapii) but differences in field performance are found between cultivars and in breeding progeny. Traditionally the degree of attack is estimated visually together with the type of reaction on the leaves. A more precise and less subjective method of evaluation is of interest for screening germplasm. A method of drying leaves, scanning dried leaves and a digital picture analysis protocol was developed to provide exact data on the percentage area of attacked necrotic tissue per leaf and with options of analyzing size distribution of single necrotic spots to reveal detailed patterns of infections. The new method was used to characterize leaf attack in autumn on 20 individual leaves from single trees from a sour cherry breeding genepool of over 100 genotypes in a non-sprayed situation with high inoculum present. The percentage of necrotic leaf surface varied highly within the 20 leaves from one genotype, being dependent on the level of inoculum dispersal and air humidity/precipitation over time and space. The mean percentage of attacked leaf surface varied from 0 to almost 35 % of total leaf area and the maximum from 0 to 60 % necrotic leaf area, indicating the 90 III International Symposium on Horticulture in Europe – SHE 2016 potential large impact on photosynthetic capacity in attacked trees. The distribution of and absolute size of single necrotic spots demonstrated differences in how individual genotypes react to infections, from small spots in tolerant genotypes to large collapsing necrotic spots in very susceptible genotypes. A comparison of attack data with the percentage of remaining leaves on a tree in autumn revealed that some genotypes retain leaves very late even if attacked by leaf spot disease. These trees may maintain a fairly high photosynthetic capacity even if some percentages of the leaf have become necrotic. T4-17 DIRT-QPCR FOR MOLECULAR BIOLOGICAL DETECTION OF RNA ENCODED VIRUSES Johanna Stammler 1, Johannes Hadersdorfer 1, Adolf Kellermann 2, Dieter Treutter 1 1 D“rnast , Freising, Associate Professorship for Fruit Science, Center for Life and Food Sciences Weihenste, Germany 2 Am Gereuth 8, IPZ3a,b, Freising, Germany For the propagation of horticultural but also agricultural plant material, a phytosanitary virus screening is obligatory for certification in order to avoid the spread of viruses regionally but also globally. To date, most high throughput standard screenings are still based on the immunological detection method ELISA because of its robustness, stability and very good cost-benefit ratio. However, in some cases molecular biological applications like PCR, qPCR or next generation sequencing are progressively or compulsorily required. For instance, low virus titers present in seed potatoes require a long virus enrichment phase for adequate virus detection by ELISA. This time consuming step can be avoided by a change to more sensitive molecular biological techniques. In case of viroid detection, antibody based methods fail due to missing epitopes. Most nucleic acid based detection methods accompany with high costs for their performance. We introduce a TaqMan™ qPCR based one step protocol named Direct Reverse Transcriptase quantitative Polymerase Chain Reaction (DiRT-qPCR)) for the detection of RNA encoded viruses without sophisticated nucleic acid purification at lower costs but with high throughput potential exemplarily on ssRNA(+) Potato leafroll virus (PLRV). T4-18 JOB SATISFACTION AND PREFERENCES REGARDING JOB CHARACTERISTICS OF VOCATIONAL AND MASTER CRAFTSMAN SCHOLARS AND HORTICULTURE STUDENTS IN GERMANY Stephan Meyerding Herrenhaeuser Str. 2, 30161 Hanover, Germany Labor costs account for 40% of all the costs in German horticulture. Employee satisfaction has a significant impact on the economic and social sustainability of horticultural companies. Traditional linear relationships between environmental characteristics and subjective job satisfaction have been assumed in psychological research. Warr challenged this conception with the non-linear vitamin model. The present study examines the possibility of non-linear relationships between job characteristics and job satisfaction. For this purpose, a survey was carried out using an online and a paper-and-pencil questionnaire from August 2013 to February 2015. In addition, the preferences of "average" employees (N=229), vocational and master craftsman scholars (N=205) and students of horticulture science (N=204) regarding job characteristics were examined. This article focuses on the later two. The relationships between characteristic values and work and life satisfaction are analyzed and the results of the three groups are compared. The strongest relationships with job satisfaction can be observed for vocational and master craftsman scholars for the job features emotional dissonance and considerate leadership. Additionally, employers' fair treatment of the society as a whole has a strong impact on job satisfaction. The salary can be found only in the lower middle range. The data support the assumption of non-linear relationships between job characteristic values and satisfaction. Furthermore, the personal characteristics of the study participants are included in the analysis. The study indicates a 91 BOOK OF ABSTRACTS change in the preference structure of employees, who in the future would prefer a good work–life balance in particular as well as other "soft" factors. T4-19 NEXT GENERATION SEQUENCING TECHNOLOGIES FOR THE DEVELOPMENT OF MOLECULAR MARKERS AND THE ANALYSIS OF GENOME DIVERSITY IN CAPSICUM SPP Teodoro Cardi 4, Nunzio DAgostino 1, Concita Cantarella 1, Vincenza Colonna 2, Barbara Greco 1, Rachele Tamburino 3, Francesca Taranto 1, Nunzia Scotti 3, Pasquale Tripodi 1 1 CREA-ORT, Pontecagnano, Italy 2 CNR-IGB, Napoli, Italy 3 CNR-IBBR, Portici, Italy 4 CREA-ORT, Centro di ricerca per l'orticoltura, via Cavalleggeri 25, 84098 Pontecagnano, Italy The accessibility and use of natural genetic variability in crops is essential for the management of genetic resources and the establishment of innovative breeding programs. Nuclear whole-genome sequences of several cultivated and wild peppers have been recently generated in order to provide crucial information on the evolution, domestication and divergence of pepper species (Capsicum spp.). Despite Next Generation Sequencing (NGS) technologies provided a significant advancement in high throughput data generation, the analysis of a large number of individuals is still challenging. An alternative strategy to Whole Genome Sequencing (WGS) is to generate a reduced representation of the genome using target enrichment strategies. Among these, Genotyping by Sequencing (GBS) provides a rapid, highly informative, cost-effective tool for exploring genetic diversity on a genome-wide scale. We applied GBS for SNP marker discovery in nuclear genomes and population structure analysis in a collection of cultivated and domesticated peppers (Capsicum spp.). Information generated in this study represent the first step towards future genome-wide association mapping studies and marker-assisted selection programs. In addition, results from this analysis can be used to gain a better understanding of the genetic relationships within the Capsicum genus. In order to provide a further contribution to that issue, we decided also to determine the complete chloroplast DNA (cpDNA) sequence of eleven Capsicum accessions for a total of 8 species belonging to the C. annuum (6), C. baccatum (3) and C. pubescens/eximium complex (2). We performed comparative analysis and identified potential molecular markers able to discriminate among Capsicum species. 92 III International Symposium on Horticulture in Europe – SHE 2016 Oral Topic – 5 Ornamentals, Landscape And Urban Environment 93 BOOK OF ABSTRACTS T5-O1 AROMATIC AND MEDICINAL PLANTS VEGETATIVE PROPAGATION USING REDUCED-COST NURSERY FACILITIES Orlanda Viamonte Póvoa, Noémia Farinha, João Paulo Mendes, Anselmo Vitorino Escola Superior Agr|ria de Elvas, )nstituto Politécnico Portalegre, Edifício do Trem Auto - Av. 14 de Janeiro, 7350 903 Elvas, Portugal The stem cuttings survival depends greatly from favourable conditions of temperature and humidity of the substrate and the air. The main goal of this study was to evaluate the survival and growth of stem cuttings of several medicinal and aromatic plants species (MAP) at the shelter located in the green spaces of ESAE without temperature control, without protection from the wind and with an expeditious watering system, using 3 daily watering periods; conditions that can be commonly found on MAP farms in the beginning of activity. The test took place between April and July 2015. Three different types of cuttings (each with four repetitions of 10 cuttings) for each species were prepared. The results of average cutting survival were greater than 90% for the species Rosmarinus officinalis L. (99%), Lavandula dentata L. (98%) and Mentha cervina (95%); being greater than 65% for Lavandula x intermedia Emeric ex Loisel. (77%) and Salvia officinalis L. (65%). The results were significantly lower p . for Aloysia triphylla L'(ér. Britton % and Thymus vulgaris 'Variegata' % , mainly due to unfavorable wind conditions during the rooting period of the cuttings. For L x intermedia survival results were significantly higher for terminals herbaceous cuttings (98.8%) compared with basal hardwood cuttings (32.5%); for Aloysia triphylla, the survival results (%) were significantly higher p . for semi-hardwood cuttings; for T. vulgaris the best results were obtained with hardwood cuttings with stalk. For R. officinalis and L. dentata no significant differences in survival rates were found for the 3 types of tested cuttings. The results indicate that it is possible to produce different MAP species, with high satisfactory survival rate, from stem cuttings with low-cost nursery infrastructures in spring in the Alto Alentejo region. T5-O2 SURVIVAL AND ROOTING CAPACITY OF ORNAMENTAL CUTTINGS AS RELATED TO TOTAL NITROGEN, STORAGE CONDITION AND SHIFT IN METABOLIC N POOLS Inga Matzner, Uwe Druege, Siegfried Zerche Leibniz-Inst. of VegetablOrnamental Crops, Department Plant Nutrition, Kuehnhaeuser Strae 101, 99090 Erfurt, Germany In the course of vegetative propagation, cuttings of many ornamental species are subjected to critical dark storage particularly during their transport to distant rooting stations. Carbohydrate and nitrogen reserves in the cutting, as modulated by factors like N supply to stock plants and light conditions, greatly influence formation of adventitious roots (AR) and propagation success. To evaluate how storage affects the cutting quality, we assessed leaf senescence and survival, nitrogen allocation, as well as the rooting response of Chrysanthemum indicum and Pelargonium zonale cuttings. Cuttings of stock plants cultivated with graduated N fertigation (low / moderate / high) were either planted immediately after harvest or first exposed to dark storage at high or low temperatures °C vs. °C for a short or extended period (3d vs. 6 / 7 d). Stock plants and cuttings during rooting received an average daily light integral of and mol/ d*m , respectively. )n parallel to assessing the survival and AR formation, composition of the metabolic N pools (i.e. soluble amide N, nitrate N, amino N, insoluble protein N) was determined using a modified Kjeldahl analysis. The chlorophyll content of the second oldest leaf, calculated indirectly via SPAD readings, was chosen as an indicator for leaf senescence. A prolonged warm, dark exposure accelerated leaf senescence and decreased the survival during rooting in Pelargonium. However, cuttings surviving under those conditions showed a higher number and length of AR with both genera. This behaviour was stimulated at raised N supply and accompanied by a decrease in 94 III International Symposium on Horticulture in Europe – SHE 2016 protein N content in favour of mobile N compounds, indicating a stronger N mobilization compared to absent or shorter and colder storage. Consequently, the applicability of different N compounds as early predictors for cutting performance after storage is discussed. T5-O3 IMPACT OF ETHANOL AND ACETALDEHYDE ON CUT CARNATION (DIANTHUS CARYOPHYLLUS L. CV, YELLOW CANDY) Asghar Ebrahimzadeh Faculty of Agriculture, Postharvest Physiology of Ornamental Plants, Department of Horticultural Sciences, University of Maragheh C.P.:55181-83111, Iran The effects of continuous ethanol and acetaldehyde treatment on cut carnation flowers (Dianthus Caryophyllus L. cv. Yellow candy) was investigated. Flowers was treated continuously with ethanol and Acetaldehyde concentrations of (2,4 and 6 %) and *0, 0.25, 0.5 and 1 %) , respectively. Some physiological indexes including the vase life, changes in fresh weight and total protein content of petals were measured. The results showed that the ethanol 2% was the most effective treatment in enhancing the vase life (20.4 Days) comparing untreated flowers with (11 days), where, total protein content was decreased in ethanol and acetaldehyde treated flowers. Postharvest treatment of flowers by ethanol and acetaldehyde t low concentrations prolonged the vase life of cut carnation by inhibiting of ethylene production and action as well it decreased ovary development. T5-O4 EFFICIENT ADVENTITIOUS ROOT FORMATION IN PETUNIA HYBRIDA CUTTINGS: WHEN SIGNALS MEET RESOURCES Uwe Druege 1, Yvonne Klopotek 1, Siegfried Zerche 1, Mohammad-Reza Hajirezaei 2, Huaiyu Yang 1, Philipp Franken 1, 1 Leibniz Institute of Vegetable and, Ornamental Crops, Erfurt, Germany 2 Leibniz Institute of Plant Genetics and, Crop Plant Research, Gatersleben, Germany Each year in Europe, several hundreds of million cuttings are rooted for production of ornamental young plants in great diversity. This clonal propagation involves a complex chain including cutting production at low latitude sites and storage and transport of cuttings to rooting stations. We have established Petunia hybrida as model to elucidate important factors and processes controlling adventitious root (AR) formation in cuttings at molecular physiological level. Involving analyses of plant hormones by GC-MS/MS, of gene expression by microarray and qPCR, and of different metabolites and related enzyme activities, we focus on the contribution of plant hormone pathways, of carbohydrate and nitrogen metabolism and the interaction of both to AR formation. Broad transcriptome analyses revealed a general stimulation of the ethylene-related pathway particularly at the levels of aminocyclopropane synthesis and oxidation and of ethylene responsive transcription factors. However, genes controlling polar auxin transport, auxin conjugation and signalling via the TIR1/AFB-Aux/IAA-ARF interaction showed phase-specific responses indicating particular functions during the induction and differentiation of ARs. Blocking of polar auxin transport eliminated the 24h-peak of indole-3-acetic acid in the rooting zone, and there reduced the activities of cell wall and vacuolar invertases and severely inhibited AR formation. Enhanced AR formation after cold dark storage of cuttings was associated with rooting zone dominated activation of cell wall invertase and induction of one particular member of the cell wall invertase encoding gene family. Together with data from analyses of amino acids, plant hormones and related transcripts, these results point towards a coordinated remobilization of nitrogen within the cutting, establishment of the new sink in the rooting zone and activation of hormone-related pathways during the dark phase. A conceptual model on hormonal and metabolic factors and processes controlling AR formation and its dark response in cuttings is presented. 95 BOOK OF ABSTRACTS T5-O5 INCLUSION IN GERMAN HORTICULTURE Robin King1, Meike Rombach, Vera Bitsch Alte Akademie 16, Oekonomik des Gartenbaus u. Landschaftsbaus, Technical University of Munich, 85354 Freising, Germany; For the past decade, a shortage of qualified labor is among the frequently discussed topics in German horticulture. Horticultural businesses report increasing difficulties finding suitable trainees, willing and able to complete a traditional 3-years apprenticeship. Despite efforts to attract young people into this field of work, the education of mentally challenged people as workforce in German horticulture remains largely ignored throughout the debate. Prior experience showed that horticulture is a suitable area of work for mentally challenged people. However, digitalization, mechanization, and a downturn in the number of apprentices have cast doubt, if horticulture is still suitable for mentally challenged employees. This study investigates drivers and barriers training mentally challenged people as skilled labor. From June through September 2015, 12 in-depth interviews with employers and their employees in ornamental and vegetable production were conducted. Companies of different size, different type of production, and different experiences with apprenticeships were targeted to participate in the study. All interviews were audiorecorded, transcribed verbatim and analyzed, using qualitative content analysis. Employers and employees interviewed reported both positive and negative experiences when training mentally challenged employees. They emphasized personal values, such as family tradition, social motivations, and the joy they get from teaching. Financial aspects were simultaneously seen as drivers of and barriers to inclusion. Employers reported cooperating with training centers and schools, as these cooperations are supportive to the professional development of mentally challenged people. Often the collaboration between employers and potential apprentices starts with an internship, and is then continued with an apprenticeship, up to its successful completion. Different from the traditional apprenticeship, mentally challenged people are trained to work on a variety of horticultural tasks, and provided with knowledge and training adjusted to their handicaps. The employers interviewed explained that mentally challenged employees often struggle to achieve the necessary plant cultivation knowledge. However, they recognized the practical learning achievements. Contrary, employees interviewed stated that their mentally challenged colleagues at times slow down the work flow. Some employers interviewed considered modern business structures and a high degree of mechanization as barriers hindering inclusion. Moreover, they emphasized the need for financial support from the government. They perceived the training of mentally challenged people as otherwise not affordable, given that apprentices with special needs result in additional monetary costs and time spent for their education. T5-O6 RE-INVESTIGATING SUBSTRATE SPECIFICITY OF DIHYDROFLAVONOL 4REDUCTASE WITH RESPECT TO THE B-RING HYDROXYLATION PATTERN OF SUBSTRATES Heidi Halbwirth, Silvija Miosic, Malvina Milosevic, Jana Thill, Christian Gosch, Karl Stich Getreidemarkt 91665, A-1060 Wien, Austria Anthocyanins carry 1-3 hydroxyl groups in the B-Ring. The most common anthocyanins are based on pelargonidin (1 hydroxyl group, orangered), cyanidin (2 hydroxyl groups, red) and delphinidin (3 hydroxyl groups, violet to blue). The hydroxylation pattern in the B-ring basically depends on two factors, the presence of enzymes introducing hydroxyl groups in the B-ring vicinal to the hydroxyl group in position flavonoid -hydroxylase and flavonoid , -hydroxylase) and the substrate specificity of dihydroflavonol 4reductase (DFR), which produces the immediate precursors for anthocyanidin formation. Important ornamental plants such as carnation, rose and pelargonium suffer from the absence of blue and violet varieties, whereas petunia, tobacco and cyclamen do not naturally produce orange and bright-red flowers. Biotechnological manipulation of flower colour (e.g. orange petunia, blue carnation, blue rose) usually alters the naturally occurring hydroxylation pattern of anthocyanin pigments by exploiting the substrate specificity 96 III International Symposium on Horticulture in Europe – SHE 2016 DFR, a key enzyme providing the precursors for anthocyanin formation. However, the molecular background for DFR substrate acceptance still remains a puzzle. Recently we presented a highly selective DHK preferring DFR from Fragaria, as a novel DFR type in addition to the well-known unspecific DFRs and those that do not convert dihydrokaempferol (DHK). We compared amino acid sequence and substrate specificity of DFRs from different plants and identified four naturally occurring types of DFRs: (1) unspecific DFRs (accepting all types of dihydroflavonols), (2) DHK rejectors (requiring more than one hydroxyl groups in ring B), (3) DHK preferring DFRs, and (4) DHM preferring DFRs. Using a DFR pair with contrasting substrate acceptance from strawberry (Fragariasp.) we show that the manipulation of few amino acids in two substrate binding regions is sufficient to switch between the different DFR types. Our results will promote the understanding how the distinct hydroxylation patterns of flavonoids are established in different species or tissues. T5-O7 INTERDISCIPLINARY RESEARCH IN URBAN AGRICULTURE Giorgio Prosdocimi Gianquinto, Francesco Orsini Dip. Scienze e Tecnologie Agroambientali , DiPSA, Universit{ degli Studi di Bologna, Viale Fanin, - 40127 Bologna , Italy In recent years, due to the increasing relevance of urban agriculture in world cities, a number of researches addressing the different associated subjects have been conducted. When plants are grown inside cities, risks may arise in term of produce safety. On the other hand, reducing food miles may result in energy and cost saving, as well as promoting city resilience. Overall, urban agriculture may provide a substantial element for ensuring both adaptation and reduction of climate change effects. Plants promote microclimate regulation and improve air quality. When green structures are integrated in buildings, they may reduce thermal conditioning costs, as well as provide noise barriers. On the other hand, highly dense urban fabric may lead to experiment cultivation in spaces that have not previously meant for hosting plants, e.g. rooftop greenhouses or artificially illuminated indoor environments. Urban green infrastructures, moreover, may improve building and neighborhood property values, and the wide recognition of the economic role played by urban agriculture in today s cities has promoted the growth and diversification of different associated business models. From a social perspective, urban gardens are commonly promoted by municipalities for their positive effects on community building and social inclusion. Furthermore, when urban gardens are properly managed and distributed within the city, they may preserve and promote urban biodiversity. At RESCUE-AB, research addresses the different elements of urban agriculture, aiming at understanding and promoting sustainable cultivation in cities. T5-O8 EDUCATION NEEDS AND RESOURCES FOR URBAN AGRICULTURE ENTREPRENEURSHIP Emmanuel Geoffriau 1, Francesco Orsini 2, Bernd Poelling 3, Femke Hoekstra 4, Mattia Accorsi 2, Rémi Kahane 6, Morgane Yvergniaux 7, Wolf Lorleberg 3, Marielle Dubbeling 4, Henke Renting 4, Francesca Magrefi 5, Giorgio Gianquinto 2, 1 Agrocampus Ouest - IRHS, 2 rue Le Notre, 49045 Angers, France 2 University Bologna D. Agricultural Science, Viale Fanin 44, 40110 Bologna, Italy 3 SWUAS - Fachhochschule S“dwestfalen , )serlohn, Germany 4 RUAF, Kastanjelaan 5, 3833 An Leusden, Netherlands 5 STePS, Via Ettore Bidone 6 , 40134 Bologna, Italy 6 CIRAD - Dept Persyst, TA B-DIR09 Avenue Agropolis, 34398 Montpellier, France 7 Vegepolys, 3 rue Alexandre Fleming, 49066 Angers, France In recent years, the growing interest towards Urban Agriculture has led to various forms of activities and innovative businesses. The European Erasmus+ Project URBAN GREEN TRAIN (URBAN GReen Education for ENTeRprising Agricultural Innovation) aims at encouraging pioneering business oriented initiatives on Urban and periurban Agriculture based on knowledge exchange, mutual cooperation and innovation among small and medium size enterprises, policy makers and (igher Education )nstitutions. The project s general 97 BOOK OF ABSTRACTS objective is to strengthen the knowledge triangle between education, research and business in the field of urban agriculture. URBAN GREEN TRAIN aims to design and test training modules and educational resources on key areas relevant for urban agriculture entrepreneurship. In this regard, we have conducted a survey of existing initiatives, educational resources or training modules related to urban agriculture and agricultural entrepreneurship among URBAN GREEN TRAIN partners but also in other organizations. The training needs related to urban agriculture context were also surveyed in companies, higher education institutions and policy makers in four European countries. A synthesis of these surveys and lessons for educational resources development will be presented. T5-O9 THE IMPACT OF THE EU VAT DIRECTIVE FOR FLOWERS AND PLANTS ON THE ORNAMENTAL SUPPLY CHAIN Frank Bunte 1, Michiel Van Galen 2 1 Tegelseweg 255, 5912 BG Venlo, Netherlands 2 Alexanderveld 5, 2585 DB Den Haag, Netherlands Because of the economic downturn, governments all over the world try to raise government revenues and to cut back government expenses. For this reason, some European governments reconsider the lower VAT tariff for ornamentals. The EU VAT directive allows Member States to apply the lower VAT rate to ornamentals. This paper evaluates the impact of a possible application of the general VAT rate to ornamentals on turnover and employment in the Dutch and European ornamental supply chain. The effects are calculated for the scenario in which the Netherlands decide to apply the general VAT rate unilaterally and the scenario in wich all member states of the European Union (EU) decide to apply the general VAT rate. In 2015, the lower VAT rate is applied to ornamentals in 13 EU member states. The impact is measured using the Hortus model of LEI Wageningen UR. Hortus models demand and supply in European horticulture. The paper shows that, if all EU Member states apply the general VAT rate to ornamentals, net sales would drop by 3.8 billion euro in EU retail trade, by 2.8 billion euro in EU wholesale trade and by 1.3 billion euro in EU horticulture. Employment would fall by 24,700 FTE in EU horticulture and by 30,000 FTE in European wholesale and retail trade. 98 III International Symposium on Horticulture in Europe – SHE 2016 Poster Topic – 1 Human Health, Nutritional Value and Horticulture 99 BOOK OF ABSTRACTS T1-P1 A SUPERIOR ANTIOXIDANT BIOMATERIAL FROM TOMATO PEEL WASTES FOR BIOTECHNOLOGICAL APPLICATION: OPTIMIZATION APPROACH BY RESPONSE SURFACE METHODOLOGY Alfredo Aires1, Rosa Carvalho2 1Univ. de Tr|s-os-Montes e Alto Douro C)TAB , Bairro de Vilalva, Rua João BatistaRibeiro , -403 vila Real, Portugal; Department, ECAV, University of Tr|s-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, Portugal 2Agronomy Every year tomato processing industry produces large amounts of solid residues including peels and seeds, without any further usage creating an environmental problem. These residues are naturally rich source of lycopene, carotenes and other important bioactive compounds and pigments, and as consequence, most of these compounds in tomatoes are wasted. Therefore, the use of this residues to extract such valuables compounds might be a good alternative to the valorisation of this by-product. In addition, the systematic utilizations of toxic organic solvents such as isopropanol, chloroform, acetone, ethyl-acetate, hexane or even methanol to extract these compounds leads to another environmental issue. In this context, we set an experimental procedure in which we evaluate the possibility of using water and ethanol at different ratios, temperatures and time of extraction in order to maximize the extraction of lycopene and -carotene without using toxic solvents. A response surface methodology (RSM) was used to establish the optimal extraction conditions. Tomato peels wastes from processing industry were used as raw material and the reaction parameters studied were: solvent ratio (20, 40, 60 and 80%), solid:liquid ratio (5, 10, 15, 20, 25 mg:mL-1), temperature of extraction , , , , ºC and time (10, 20, 30, 40 minutes). The total carotenoid was assessed by colorimetric methods and individual carotenes was assessed by HPLC-DAD/VIS-UV. Our results showed that of water and ethanol at different ration provides a feasible method to extract considerably amounts of lycopene and -carotene reducing the cost of the entire extraction process if compared with the commonly organic used solvent-based procedure. The effective utilization of these by-products might help the prevention of pollution problems and may offer new possibilities for extraction of bioactive compounds using environmental friendly solutions. T1-P2 ALLERGENIC POTENTIAL OF LOCAL, SCAB RESISTANT, RED FLESHED AND COMMERCIAL APPLE CULTIVARS Thomas Letschka, Valentina Cova, Pietro Delfino, Giulia Pagliarani, Walter Guerra, Michael Oberhuber Laimburg 6, 39040 Ora, Italy; 2University of Bologna, Viale Fanin 44, Bologna, Italy The consumption of fresh fruits and vegetables is highly indicated for a healthy and balanced diet. Particularly apples are recommended due to their high content in vitamins, polyphenols and antioxidants, especially in the apple peel. However, in the past decades apples have emerged continuously as one of the principal foods that cause IgE-mediated allergy. Symptomes are associated to the oral allergenic syndrome (OAS) immediately after the ingestion of fresh, unprocessed and unpeeled apples. Thus, susceptible consumers are forced to cook or peel apples before consumption, resigning at the same time to an important portion of the apple s beneficial components. For these reasons, the quality and attractiveness of an apple is also linked to his allergenicity and running breeding programmes focus more and more on low allergenic impact as selection criterion for the development of new varieties. To deepen the knowledge on the genetic and physiological background behind the synthesis of apple allergens is therefore of essential importance. Apple allergy is triggered by four different allergen families pertaining to the PR proteins (Mal d 1, Mal d 2, Mal d 3) or the profilins (Mal d 4). As first step of allergen production, the mRNA synthesis of the respective genes have to be investigated. In this study, the gene expression of all 31 members of the Mal d 1 gene family has been determined in different fruit tissues of 25 apple accessions after cold storage. Accessions are grouped into (i) commercial, (ii) old and local, (iii) red fleshed and (iv) scab resistant cultivars carrying the Rvi6 resistance gene. As expected, expression levels in the peel were significantly increased compared to the fruit cortex in almost all cultivars. None of the four cultivar groups had a significantly increased or reduced gene expression compared to the other groups. 100 III International Symposium on Horticulture in Europe – SHE 2016 T1-P3 ALTERNATIVE USE OF FRUIT AND ITS WASTE STREAMS IN PHARMA Marijke Jozefczak, Tessa De Baets, Tom Deckers, Proefcentrum Fruitteelt Fruittuinweg 1, 3800 Sint Truiden, Belgium; Due to the increasing expectations and standards of both merchants and consumers, imperfect fruits are often discarded or sold at very low prices. This project aims to both reduce food losses and support fruit growers by providing alternative uses of these fruits. One possibility is the extraction of fruit components with health-promoting properties, also called nutraceuticals. In the last few years, many research has focused on the positive influences of fruit on neurodegenerative diseases. This study focuses mainly on polyphenols, secondary plant metabolites that are generally involved in defence mechanisms. A detailed literature study was performed to find a match between polyphenols that (1) are present in high concentrations in local fruits and possess a preventive and/or therapeutic activity against Alzheimer s and Parkinson s disease, two major types of dementia. In conclusion, anthocyanins (cyanidins and pelargonidins), catechins, quercitins, ellagic and caffeic acids were selected for further studies. On the one hand, publications revealed high levels of these compounds in apple, pear and strawberries. Important to take into account is the major influence of the extraction method on these concentrations. On the other hand, literature associates these compounds with both general antioxidant, anti-inflammatory and anti-apoptotic functions and specific antiaggregation actions directed towards proteins relevant in Alzheimer s and Parkinson s disease such as amyloid-bèta and tau proteins. (owever, it is essential to consider the influence of human metabolism (ADME: absorption, distribution, metabolism and excretion) on the final pharmacological effect of each compound. Currently, experimental setups are performed to confirm the theoretical background and determine the toxicology of different polyphenols. In parallel, extraction methods are explored in order to maintain the highest levels of interesting compounds. T1-P4 CHANGES OF CHLOROPHYLLS, ANTHOCYANINS AND ANTIRADICAL ACTIVITY IN NETTLE (URTICA DIOCA L.) LEAVES DURING VEGETATION PERIOD Solvita Zeipina1, Liga Lepse2,Ina Alsina1 1Liela street 2, Jelgava, Latvia; street 1, Dobele, Latvia 2Graudu Consumers more and more are interested in healthy and balanced diet. Stinging nettle (Urtica dioca L.) leaves traditionally are used in early spring as a leafy vegetable in salads and soups. Leaves are a good source of some important minerals and vitamins. The investigations are carried out at Institute of Horticulture, Latvia University of Agriculture in 2015 and 2016. The research was aimed to evaluate the biochemical composition in four different stinging nettle clones of local origin. Investigation was carried out in two different soils and under two growing technologies. Two harvesting approaches where used in the trial: 1) 7 – 10 cm long shoots cut five times and 10 – 15 cm long shoots cut two times during vegetation period. Content of chlorophylls and anthocyanins, as well as antiradical activity were detected in Latvia University of Agriculture, Institute of Soil and Plant Science laboratory. Higher content of chlorophylls was obtained in 2016. Content of anthocyanins reduces during vegetation period. Significantly higher content of anthocyanins was observed in 2016. Antiradical activity in both years ranged between 15 – 60%. 101 BOOK OF ABSTRACTS T1-P5 DISPERSAL OF E. COLI O157:H7 GFP+ IN SPROUTING MODULES Emina Mulaosmanovic, Sebastian Farkas, Julia Lindén, Maria Sousa, Samareh Gharaie, Lars Mogren, Andrea Kosiba Held, Thabani Eddington S. Chirere, Beatrix Alsanius Swedish University of Agricultural Sciences, Department of Biosystems and Technology, P.O. Box 103, 230 53 Alnarp, Sweden One of the most harmful foodborne human pathogens, Escherichia coli O157:H7, has been in the spotlight several times during the last decades due to its infectivity and epidemic nature of spreading. Recent outbreaks of E. coli infections associated with consumption of raw vegetables, predominantly sprouted seeds, have been reported in several countries around the world. At the same time growing and raw consumption of sprouted seeds is associated with a healthy lifestyle in Scandinavia, and different types of sprouting modules for home production are available. During sprouting ideal conditions for emergence, but also for bacterial proliferation are prevailing. As the seeds are non-sterile, also human pathogens may propagate. We studied the dynamics of E. coli O157:H7 gfp+ in two types of three-layered sprouting modules using organic fenugreek seeds (Trigonella foenum-graecum) as a model crop. E. coli O157:H7 gfp+ was introduced to either bottom or top layer of the modules and its dispersal was analyzed after 3.5 days. We found that the inoculated strain moved both vertically up and down between the layers. High hygiene standards are needed for both seeds and modules used for home sprouting of seeds. T1-P6 EFFECTS OF NITROGEN LEVEL ON PURSLANE ANTIOXIDANT ACTIVITY Sofia de Jesus1,Ricardo Santos2, Isabel Alves-Pereira3, Rui Machado2,Rui Ferreira3 2Dept. 1Dep. Química, Universidade de Évora, Évora, Portugal; De Fitotecnia, Univ.de De Evora, 7000 Evora Codex, Mitra, Portugal; 3)CAAM, Universidade de Évora, Évora, Portugal. -554 The high antioxidant activity of purslane, Portulaca oleracea L., gives it a high nutritional and functional value. The commercial production of purslane has increased in Portugal, making it necessary to know the effects of inputs, mainly nitrogen, on the antioxidant activity. The main goal of this study was to evaluate the influence of nitrogen application on purslane antioxidant activity. The experiment was carried out with for treatments: 30, 60 and 90 kg/ha of nitrogen. Plants of golden-leaf purslane were grown in Styrofoam-boxes filled with substrate and fertigated two times per week, over four weeks with ammonium nitrate solution (16.9% NO3-N and 16.7 NH4+-N). The increase in the nitrogen level decreased the water-soluble proteins content. However the ascorbate, phenols content as well as antioxidant activity measured by FRAP method was not affected by nitrogen level. Plants shoot antioxidant activity, measured by DPPH method decreased significantly in the treatment with 90 kg N/ha (26.20 mg/g gallic acid). On the other hand, plant shoot antioxidant activity mediated by peroxidases was higher in treatment kg N/ha . µmol min-1/mg prot.). Application of 60 kg N/ha allowed a vigorous plant growth without disturb its antioxidants and conservation properties. T1-P7 ELIMINATION OF PRUNE DWARF VIRUS FROM SWEET CHERRY CULTIVARS BY IN VITRO CHEMOTHERAPY Frantisek Paprstein 1, Jiri Sedlak 1, Jaroslav Polak 2, Shesh Kumari 2 1 Research and Breeding Inst. Pomology, Holovousy, 50801 , Horice, Czech Republic 2 Crop Research Institute, 161 06 Prague, Czech Republic; The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Prune dwarf virus PDV from in vitro grown plants of infected sweet cherry cultivars Tamara and 102 III International Symposium on Horticulture in Europe – SHE 2016 Amid . PDV causes malformations, decreases in vigour and in yield. PDV was detected in naturally infected field-grown trees by RT-PCR analysis. In vitro cultures were successfully multiplied on MS medium with 1.5 mg L-1 BAP (6-benzylaminopurine). Ribavirin was added in concentration of 20 mg L-1 after autoclaving to the same MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis (about 3 mm in length comprising the apical meristem plus two-three primordial leaves) was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg L-1 BAP for regeneration. The chemotherapy technique was able to eliminate PDV in different percentage according to the cultivar, varying between . % for cultivar Tamara and . % for cultivar Amid . Phytotoxicity of ribavirin was not observed in the examined cultivars. The obtained results demonstrate the effectiveness of the system of virus elimination by a combination of in vitro cultures, chemotherapy with ribavirin and subsequent removing of apical meristematic region. Relatively high percentage of obtained virus free plants confirms high antiviral activity of ribavirin against PDV. Repeated testing of established plants is necessary to ensure that the propagation material in certification programs remains virus-free. T1-P8 FIRST RESULTS ON POMEGRANATE PROCESSING FOR JUICE EXTRACTION Pietro Catania, Maria Alleri, Claudio De Pasquale,Mariangela Vallone Viale delle Scienze ed. 4, Palermo, Italy. Pomegranate juice has got many health properties because the fruits contain anticarcinogenic, antimicrobial and antiviral compounds. Its consumption has greatly increased throughout the world in recent years due to the potential of its different components, polyphenols and anthocyanins among all. Many studies have been performed on the pomegranate juice yield demonstrating its influence on the organoleptic and physicochemical properties of the juice. Commercial pomegranate juice production involves the pressing of the fruits. As a consequence, there is a need to investigate the pressing machine types and adjustment in order to maximize juice yield and enhance its health properties. The aim of this study is to assess the influence of the pressing system on the pomegranate juice yield and properties. Pomegranate fruits of the Wonderful One variety were manually harvested in November 2014 and mechanically processed to extract juice. Two different pressing systems were used. The first one was a hydraulic pressing machine where the fruits were put after being sliced in half; the second extraction method consisted in using a destemming machine for the entire fruit, followed by a pneumatic press, typically applied in grape processing. The juices obtained with the two systems were analyzed and compared in order to identify the best extraction technique. T1-P9 FRUIT SUBTROPICAL PLANTS IN THERAPEUTIC AND PREVENTIVE NUTRITION Elena Shishkina Crimea, Yalta, Russian Federation. New kinds of therapeutic and prophylactic products, developed in Nikita Botanical Gardens (NBG). The problem of preservation of human's health, including by improving diet by foods rich in vitamins, pectin with antimicrobial, antioxidant, immune-stimulating properties has become of vital importance. Such subtropical fruit crops as zizyphus, feijoa, figs, olives are a source of biologically active substances. The new products of therapeutic and prophylactic purposes, produced by NBG are made from the fruit of subtropical crops growing in NBG collection sites. These eco-friendly and natural products one of high quality and meet all the requirements of international standards. The following products: "Honey zizyphus", "Jam of figs", "Canned - olive "," Stewed feijoa ","Stewed fruit zizyphus "and others products are rich in biologically active substances, are of high medical properties and have good taste. 103 BOOK OF ABSTRACTS "Honey zizyphus", "Stewed fruit zizyphus" are highly effective therapeutic and prophylactic products with marked hypotensive properties. They strengthen the blood vessels, help to improve the indicators of red blood cells and the immunity. These effective fortifying agents are recommended for the prevention of premature aging. "Gem of figs" contains sugar (fructose, glucose), pectin, vitamins A, B1, B2, E1, PP; minerals as Fe, P, K, Ca, Mg. "Feijoa fruit Stewed" is rich in iodine, pectin, vitamins C, P-active substances, polyphenolic compounds. The fruits of feijoa are used for the prevention of cardiovascular and endocrine diseases are product especially rich in vitamins. "Canned olive" is a valuable food and dietary product. Olives contain proteins, sugars, pectins, vitamins B, E, provitamin A, potassium, phosphorus and iron. Olive oil which is contained in the fruit (up to 75% on the dry weight of pulp) is of particular value. T1-P10 IMPACT OF PARTIAL ROOTZONE DRYING AND MULCHING ON FRUIT QUALITY AND NUTRIENT CONTENTS AFTER STORAGE OF 'POLKA' RASPBERRIES Kujtim Lepaja, Endrit Kullaj, Lavdim Lepaja Agriculture University of Tirana, Tiranë, Albania The purpose of this research was to determine fruit quality and nutrient elements in raspberry fruits after applied water stress - partial rootzone drying and mulching. Macro and micro elements of fruits were analysed after stored. The experiment was conducted in Kosovo (Prishtina region) during 2014 and 2015 in a one- to two- year old raspberry orchard of 1 ha using a nested experimental design. Four levels of budget irritation were studied: 100% ET (control) lateral, two laterals (TL), side lateral (SL), and without irrigation (WI). Each treatment contained 10 plants, of which five plants per treatment were mulched with wood chips (size 1-2 cm) at a 10 cm layer in first year of experiment while, 20 cm in second year. Using two-way ANOVA we found significant changes in a series of fruit quality parameters and macro and micro elements. Irrigation had significant changes in: number of fruits, fruit diameter, fruit length, fruit weight, pH, Brix, K, Ca, P, Mg, Mn, B, Fe, Cu, Cr, Zn, Ni and Pb. It should also be stressed that mulching had significant changes in: fruit weight, pH, Brix, K, Ca, P, Mg, Mn, B, Fe, Cu, Cr, Zn, Ni and Pb. It can be concluded that partial rootzone drying can be successfully applied to raspberry also, PRD is an ideal water saving technique. T1-P11 INDUCED PRODUCTION OF INDOL-3-YLMETHYL GLUCOSINOLATES IN HAIRY ROOTS OF CHINESE CABBAGE (BRASSICA RAPA SUBSP. PEKINENSIS): PERSPECTIVES TO ENHANCE THE CONTENT OF BIOACTIVE COMPOUNDS Alfredo Aires1, Madeleine Neumann2, Jutta Ludwig-M“ller2, Maria Schöpe3, Karl-Heinz Van Pée2 1Centre for the Research and Technology for, Agro-Environment and Biological Sciences, C)TAB, University of Tr|s-osMontes e Alto , Douro, 5001-801 Vila Real, Portugal; 2Technische Universität Dresden, Dresden, Germany; 3Vita 34 AG, 04103 Leipzig, Germany. Brassica species have an important class of secondary metabolites, the glucosinolates, whose specific hydrolysis products are critical for flavor, smell and nutritional properties. While some of the hydrolysis products have been recognized as health promoting compounds for humans, others, such as indol-3-ylmethyl glucosinolates, have been associated with deleterious effects by forming DNA adducts. Thus, it is important to have a strong knowledge of all aspects involved in the biosynthesis of such compounds in order to design vegetables with an improved secondary metabolite profile. In this context we present this study, in which Chinese cabbage was treated with different signaling substances (jasmonic acid, salicylic acid, and yeast extract and grown for weeks, under light, ºC and ºC. Metabolomics analysis, aiming at specific changes 104 III International Symposium on Horticulture in Europe – SHE 2016 in the glucosinolate and related indole derivatives profile and content, were preformed using HPLC-DADUV/VIS. Hairy roots of Chinese cabbage were studied. Preliminary results showed a variation in the profile and content of the above compounds, suggesting a variation in their biosynthetic pathway. The specific differences observed in this comparative analysis enables the development of new and innovative tools for breeding vegetables with improved GS and indole compounds in the near future. T1-P12 INFLUENCES OF SOIL AMELIORATIVE PLANT SPECIES ON THE ORGANIC EDIBLE ROSE CULTURE Ana Cornelia Butcaru, Florin Stanica Bv.Marasti 59, Bucharest, Romania. An important alternative method to increase the soil fertility and biological activity is using the ameliorative plant species, especially before planting an organic perennial crop. In the first stage of establishing an organic edible rose culture, three ameliorative plant species were used: Sinapis alba L., Tagetes patula L. and Phacelia tanacetifolia L., with role on soil disinfection. Plants were seeded in seven different combinations (V1-V7) and a control plot was kept without seeding (V8). After flowering and seed formation, the mature plants were trimmed and incorporated into the soil. After the organic roses planting, the same variants were seeded between the rose rows. The results showed an improvement of the soil microbiological activity and stimulation of the roses growth and flowering, indicated by the height and number of the branches and flowering sprouts. The agrochemical analysis indicated an increase of the soil organic matter content, potassium and pH value. T1-P13 INVESTIGATION OF ANTIMICROBIAL PROPERTIES OF DIFFERENT BERRIES Remigijus Daubaras1, Laima Cesoniene1, Antanas Sarkinas2 1Z,E, Zilibero 6, 46324, Kaunas, Lithuania; pr. 92, 51180 Kaunas, Lithuania; 2Taikos Berries are a source of natural compounds that could be widely used for different purposes due to their antimicrobial and antioxidative properties. The food industry needs organic raw that is stable to various industrial processes and prolong the shelf-life of food products. Berries of European cranberry Vaccinium oxycoccos, highbush blueberry Vaccinium corymbosum, and snowball tree Viburnum opulus are much appreciated in folk medicine also because of large amounts of biologically active substances. Different cultivars of European cranberry, highbush blueberry, and snowball tree were selected for investigations of antimicrobial properties. The antimicrobial activity of berry ethanol extracts was determined by the agar well diffusion method. Determination of the antibacterial properties showed that berry extracts inhibited the growth of wide range of human pathogenic bacteria, both gram positive and gram-negative. Investigation of the antimicrobial properties showed that V. oxycoccos extracts inhibited the growth of wide range of human pathogenic bacteria, both gram-negative (Escherichia coli and Salmonella typhimurium) and gram-positive (Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, and Bacillus subtilis). Examination of V. covilleanum berry ethanol extracts by the diffusion to agar method showed that among gram-negative test cultures Citrobacter freundii was the most sensitive. The most effective antibacterial activity was exhibited by the V. opulus berry extracts against Salmonella typhimurium, Salmonella agona, and Lysteria monocytogenes. Staphylococcus epidermidis and Micrococcus luteus displayed the highest resistance. In contrast, the growth of the yeast cultures exhibited little or no sensitivity to the berry ethanol extracts of all species investigated. Our study indicates that berries of V. oxycoccos V. corymbosum, and V. opulus may act as antimicrobials, which control a wide range of pathogens. 105 BOOK OF ABSTRACTS T1-P14 NUTRITIONAL AND FUNCTIONAL VALUE-ADDED OF BIOFORTIFIED APPLE PEELS Claudia S|nchez1, Ana Isabel Mingote1, Maria Paula Duarte2 1)nstituto Nacional de )nvestigação Agr|ria , e Veterin|ria )N)AV, Oeiras, Portugal; Faculdade de Ciências e Tecnologia , FCTUNL, Lisbon, Portugal. 2DSTB, Apple peel is naturally rich in phenolic compounds and dietary fiber. In the particular case of apples resulting from a process of calcium biofortification, peel is also particularly rich in this nutrient, essential in any healthy human diet. Biofortified apple peel is a by-product of pulps and nectars industry. In this work, we proposed that a valuable food ingredient could be made using this by-product. Golden delicious apples biofortified with calcium by three different pre-harvest treatments were used. Commercial Golden delicious apples, without calcium biofortification, were used for comparison. Apple peels were oven-dried at ºC, ground and stored in hermetically sealed bags until use. The apple peel powder obtained was characterized in terms of water, protein, ash, sugars (glucose, fructose, sucrose and sorbitol), dietary fiber, total phenolic and mineral content. Besides the expected higher concentration of calcium, peel powder from biofortified apples showed a dietary fiber content 20% higher than commercial apples. For all the samples analyzed, the total phenolic content was very high, with values ranging from 420 to 500 mg gallic acid equivalents/100 g dried peels. The biofortified apple peel powder obtained may be used as ingredient in the formulation of diverse food products to add bioactive compounds and to promote good health. T1-P15 NUTRITIVE VALUE OF MARKETABLE HEAD CABBAGE AND WASTE OUTER LEAVES OF BRASSICA OLERACEA L. VAR. CAPITATA SUBVAR. ALBA AS AFFECTED BY NITROGEN FERTILIZATION Ryszard Kosson, Stanislaw Kaniszewski, Kazimierz Felczynski, Maria Grzegorzewska, Magdalena Tuszynska Research Institute of Horticulture, Konstytucji 3 Maja 1-3, 96-100 Skierniewice, Poland. The effect of nitrogen fertilization (0, 100, 200 and 400 kg ha-1 N) on yield and nutritive value of white head cabbage cv. Transam and cv. Typhoon was studied. Contents of ascorbic acid, total sugars, soluble polyphenols, total flavonoids, nitrates and total nitrogen were assayed as well as total antiradical activity (DPPH) of marketable cabbage heads and waste outer leaves were analyzed. The total and marketable yield of both cultivars of cabbage has increased with higher dose of nitrogen during fertilization. Waste cabbage fraction (green outer leaves) of both cultivars accounted for 24.8% to 29.9% of the total mass of cabbage. With the increase of nitrogen fertilization the percentage of the outer leaves by weight of head has decreased. The higher fertilization with nitrogen caused an increase in total nitrogen content and the decrease of total sugars content in the marketable heads of cabbage. An Increase in fertilization up to 400 kg ha-1 N caused a several fold increase of nitrates level in cabbage. Higher nitrogen fertilization 200-400 kg.ha-1 N influenced on lower content of flavonoids in green outer leaves - 536 mg.kg-1 for cv. Transam and 411 mg.kg-1 for cv. Typhoon, while the lower rate of N fertilization (100 kg.ha-1) resulted in higher content of flavonoids in green outer leaves - 728 mg.kg-1 for cv. Transam and 586 mg.kg-1 for cv. Typhoon. The outer leaves of cabbage characterized by a significantly higher nutritive value compared with white leaves of marketable heads, as indicated by several-fold higher content of total flavonoids, soluble polyphenols and over the 100% increase of ascorbic acid content. 106 III International Symposium on Horticulture in Europe – SHE 2016 T1-P16 PECULIARITIES OF BIOLOGICAL ACTIVE SUBSTANCES ACCUMULATION IN PERSIMMON FRUITS Sergey Khokhlov1, Elena Dunaevskaya2, Igor Kostenko2 1Crimea, 2Nikita Russian Federation, Yalta, Russian Federation; Botanical Garden, Yalta, Russian Federation; The comparative evaluation of the chemical composition of perspective persimmon cultivars grown in Nikita Botanical Gardens, Crimea was presented in this paper. The researches have been done using standard methods. Nutritious value of persimmon fruits is determined by high content of sugars, mainly glucose and fructose, organic acids, pectins, polyphenols, minerals, vitamin C and -carotene. In conditions of the South Coast of the Crimea the persimmon fruits accumulate a small quantity of Zn. The contents of the following mineral elements were determined: potassium, magnesium, iron. Ripened fruits have tonic, astringent, antiinflammatory and antiseptic activity. Persimmon fruits are used to treat different gastro-intestinal diseases, anaemia, some gum diseases, and goiter. T1-P17 QUALITY, NUTRACEUTICAL CONTENT AND ANTIMICROBIAL PROPERTIES OF EUROPEAN CRANBERRYBUSH (VIBURNUM OPULUS) FRUITS Remigijus Daubaras, Laima Cesoniene Z.E. Zilibero 6, LT-46324 Kaunas, Lithuania. Fruits of the species Viburnum opulus L. are widely used in traditional and folk medicine. Interest in the biochemical composition of V. opulus fruits has intensified because of food industry demand for natural substances. In Russia, many Siberian nations, and Ukraine they are an ingredient in fillers, sauces, cakes, and drinks. V. opulus fruits are popular in Canada and are used as substitutes for cranberries .The aim of present study was to determine agro-biological and biochemical variability and antimicrobial properties of V. opulus fruits and to select the genotypes as the best sources of health promoting substances. Different cultivars and clones were evaluated at Kaunas Botanical Garden of Vytautas Magnus University. Fruits of the local clone P3 were characterized by large amounts of total phenolics, ascorbic acid, and reducing sugars. The amount of ascorbic acid varied from 12.4 to 41.4 mg/100g, amount of carotenoids varied from 1.4 to 2.8 mg/100g, amount of anthocyanins varied from 23.2 to 44.6 mg/100g, and the amount of total phenolics varied from 753.0 to 1460.0 mg/100g. The genotypes V. opulus var. sargentii and V. opulus var. americanum contained large amounts of total phenolics, 1460.0 and 1400.0 mg/100 g, respectively. Fruits have also been investigated for their ability to combat various bacterial and fungal diseases. The most effective antibacterial activity was exhibited by the fruit juices against Salmonella typhimurium, Salmonella agona, and Lysteria monocytogenes. The presence of biologically active compounds enables use of V. opulus fruits as potent antioxidants. We suggest, therefore, that health promoting properties of V. opulus fruits will augment the comprehension of this plant and facilitate its use in food and pharmaceuticals. 107 BOOK OF ABSTRACTS T1-P18 SEARCHING FOR A SULFUR DIOXIDE REPLACEMENT FOR TABLE GRAPES K. Howe 1, George Manganaris 4, A. Ferry-Abee 2, D. Gubler 3, C. H. Crisosto 1 1 University of California, Dept. of Plant Sciences, Mail Stop 2, One Shields Avenue, Davis CA 95616, United States of America 2 University of California, Cooperative Extension Tulare County, 4437-B S. Laspina St., Tulare CA 93274, United States of America; 3 University of California, Department of Plant Pathology, One Shields Avenue, Davis CA 95616, United States of America 4 Anexartisias 33, P.O. Box 50329, 3603 Lemesos, Cyprus Table grapes are an important industry worldwide and due to restrictions on using sulfur dioxide (SO2), there is increased interest in storage-transportation methods which do not use SO2 to control decay. The objective of this study was to determine whether an initial high CO2 treatment followed by controlled atmosphere (CA) would control decay incidence while maintaining the visual and eating quality of different table grape cultivars. Ten table grape cultivars were stored in 40% CO2 + 60% N2 for 48 hours followed by controlled atmosphere (CA) of 12% O2 + 12% CO2 + 76% N2 or air (control) for up to eight weeks in cold storage. The high CO2 treatment followed by CA was effective in preventing decay while maintaining good eating quality after cold storage plus two days shelf life (SL). Additionally, the treatment was effective in the prevention of Botrytis decay throughout four and eight weeks of storage, with residual effects of the treatment controlling decay through SL. It was hypothesized that different table grape cultivars would respond differently to the high CO2 combined treatments with some cultivars being more tolerant to the high CO treatment, not developing off-flavor . Differences in development of off-flavor would likely be due to cultivars varying in their anaerobic compensation points (ACP). When the ACP is reached, fruit cells metabolism switch from aerobic to anaerobic respiration, which results in the synthesis of acetaldehyde (Aa) and ethanol EtO( ; compounds correlated with off-flavor in other fruits. A trained panel and consumer test screened the fruit for the presence or absence of off-flavor which was only found in four of the ten cultivars after four weeks storage plus SL. Therefore, this novel high CO2 treatment followed by CA is a promising alternative to SO2 for many table grape cultivars currently in production. T1-P19 SUGAR AND TOTAL PHENOL CONTENTS OF HASS AVACADO Ayse Tulin Oz1, Mozhgan ZARIFIKHOSROSHAHI2, Hatice OZCAN2, Ebru Kafkas2 1Osmaniye Korkut Ata University, Food Engine, 80000 Osmaniye, Turkey; of Horticulture, Faculty of Agr, Adana, Turkey 2Department Avocado is one of the important fruit species and well adapted to the Mediterranean region in Turkey. Avocado fruit are not only health benefits but also their aroma, taste and nutritional value make fruits farmers started their plantations in Turkey. (ass avocado is the one of the most important avocado variety cultivated in all over the world. Thus in this study the of (ass avocado Persea americana Mill. species were evaluated based on their sugar and total phenol content. The total sugar content of avocado species was 2.52%, glucose content was 2.24%, fructose was 0.183 % , sucrose was 0.144 % and sorbitol was 0.051%. However total phenol content was 164.17 mgGAE/100g. The results indicated that avocado are rich in total phenol and glucose content. The present study revealed that the avocado fruits have a valuable levels of health beneficial phenol content and genotypes varied based on their sugar and phenol constituents. 108 III International Symposium on Horticulture in Europe – SHE 2016 T1-P20 THE EFFECT OF ORGANIC AND CONVENTIONAL FRUIT PRODUCTION METHOD ON MINERAL COMPOSITION AND SENSORY PROPERTIES OF CLOUDY APPLE JUICE Lagle Heinmaa1, Ulvi Moor2, Priit Poldma1, Tonu Tonutare1, Kersti Kahu1, Ulla Kidmose3, Roberto Lo Scalzo4 1Institute of Agricultural and Environmental, Sciences, Estonian University of Life Sciences, Tartu, Estonia; 2 Estonian University of Life Sciences, Kreutzwaldi 1, Tartu 51014, Estonia; 3Department of Food Science, Aarhus University, Aarhus, Denmark; 4C.R.E.A. Consiglio per la Ricerca , in Agricoltura e l'Analisi dell'Economia, Agraria, Milano, Italy. The aim was to determine the differences in mineral composition and sensory properties of cloudy apple juice depending on apple production method. Three indigenous Estonian cultivars were chosen on condition that the same cultivars would be grown in organic and conventional orchards at the same geographical location. Krameri tuviõun and Talvenauding apples were harvested from old over years low maintenance orchard and Krista apples from -years old experimental orchard. 100 kg of apples per cultivar and production method were stored at + ± °C for two months and then pressed into juice using Lancman waterpress. Total soluble solids (TSS), titratable acidity (TA) and content of P, K, Ca and Mg were determined from apples and from juice. Descriptive sensory analysis of apple juice was carried out by a trained sensory panel. Krameri tuviõun and Talvenauding apples and respective juices from old organic orchards had significantly higher content of P, K and Mg compared to the conventional counterparts. Mineral composition of Krista apples and juice from younger well- maintained organic orchard was not significantly different from conventional ones except for P concentration, which was higher compared to conventional apples. Organic apples from old orchards had higher TSS compared to conventional ones, but this difference was no more significant in juice. Sensory properties of juices were most of all affected by cultivar: Krameri tuviõun juice was more sweet and less sour compared to Talvenauding and Krista . Production method did not affect the sweetness, sourness as well as fresh- and apple flavour. Our results indicate that even though the old organic apple orchards do not produce high quality apples for fresh consumption, cloudy apple juice made from those apples is better source of valuable mineral elements for humans than apple juice pressed from conventional or well-maintained organic orchards. 109 BOOK OF ABSTRACTS T1-P21 TRADITIONAL GREEK PIES ("PITES"): NUTRITIONAL PROFILE ANALYSIS AND BENCHMARKING Helen Gkika 1, Kyriaki Kalinteri2, George Palisidis2, Sofia Loupassaki1, George Boskou2 1Laboratory of Analytical Chemistry, Mediterranean Agronomic Institute Of Chania, Makedonias 1, Alsyllio Agrokipiou, Chania, Greece; 2Department of Science of Dietetics – Nutrition, Harokopio University, Athens, Greece;. The traditional Greek cuisine could be considered a typical culinary model of the Mediterranean Diet (MeD). Pies in the traditional Greek cuisine, called "pites", can be cooked with different fillings (cheese, vegetables, meat), and with different pastry sheets, called "phyllo", as well as by a variety of cooking methods. There is insufficient scientific evidence for their nutritional value. Therefore, the aim of this study is to collect nutritional and culinary information on the traditional Greek pies, organize the data in a consolidated database and perform benchmarking analysis on nutritional and geographic criteria. Therefore, a total number of 350 different recipes of Greek pies is identified and registered in spreadsheets. The literature sources of the recipes were traditional cooking books and websites of Greek associations of chefs, associations and institutes on Greek folklore, and national organizations specialized in Ho.Re.Ca. For the nutritional analysis, the USDA food composition tables are used, enhanced with 2 official databases for the composition of Greek foods and dishes. Benchmarking analysis is performed with selected statistical methods such as polar charts, factor analysis, principal component analysis and clustering. The aim of the analysis is to identify nutrient variations of the similar recipes within different geographic regions as well as to identify optimal nutritional intakes by the consumption of pies. The consolidates database can become public in a website, an e-book or even an android application in order to be communicated to consumers, tourists and professionals in Ho.Re.Ca. The outcome of this study underlines the nutritional value of the Greek pies and this could be considered as a primary step for introducing healthier dietary patterns both for the modern Greeks and the tourists that visit Greece. Furthermore, by enhancing the consumption of traditional pies there can be agricultural development and sustainability of the rural economy. T1-P22 YIELD, QUALITY AND GREENHOUSE GAS EMISSIONS IN ORGANIC AND CONVENTIONAL FAVA BEAN CROPS Juan A. Fernandez 1, Virginia S|nchez-Navarro , Ra’l Βornoza , Angel Faz, Dpto . 1 Dpto. Producción Vegetal, Univ. Politécnica de Cartagena, Paseo Alfonso X))), , Cartagena Murcia , Spain 2 Dpto. Ciencia y Tecnología Agraria, Universidad Politécnica de Cartagena, Paseo Alfonso X))), , Murcia Cartagena, Spain In this study we assessed crop yield and quality and soil emissions of greenhouse gases (N2O and CH4) and their interaction with soil properties of two cultivars of fava bean (Muchamiel and Palenca) with different management practices (conventional and organic). Fava bean plants were grown within a randomized complete block study with four replications, in plots of 10 m2. Fava bean crops spanned from 24 November 2014 to 2 March 2015.Gas samples were taken indifferent times (0, 30 and 60 minutes) once a week using the static gas chamber technique. Soil samples (0-30 cm) were taken from each plot at the end of the crop cycle to measure enzyme activities such as -glucosidase, -glucosaminidase, dehydrogenase, urease, cellulose and arylesterase. Yield and quality of the crop were also determined at the end of the cycle. The highest yield was obtained in Muchamiel under conventional practice. A positive correlation between Muchamiel yield and enzyme activities such as arylesterase and cellulose was found. In addition, Muchamiel had the highest values in the number of seeds per plant, pod length and plant biomass. Organic practice contributed to reducing GHG. N2O emissions were the highest for both cultivars under conventional practice, with an average increase of 18.27 mg m-2 in Muchamiel and 8.95 mg m-2 in Palenca respect to those grown under organic practice. CH4 emission was the highest in Palenca under conventional practice, with an average increase of 455 mg m-2 over this cultivar grown organically. We observed significant negative 110 III International Symposium on Horticulture in Europe – SHE 2016 correlations between N O emissions and -glucosaminidase activity. CH4 emission showed a positive correlation with the enzyme activities arylesterase and cellulose. Pod length showed a positive correlation with N2O emission. Conventional practice increased fava bean yield, although also enhanced N2O and CH4 emissions. 111 BOOK OF ABSTRACTS Poster Topic – 2 Quality, Authentication, Traceability & Supply Chains 112 III International Symposium on Horticulture in Europe – SHE 2016 T2-P1 ACETAMIPRID IN APPLES, DEGRADATION AND RESIDUES Sanja Lazic, Dragana Sunjka, Slavica Vukovic, Srdjana Petrovic University of Novi Sad, Faculty of Agriculture, Trga Dositeja Obradovica 8, 21000 Vojvodina Novi Sad, Serbia; Apple is one of the most important fruits species in Serbia, covering 23.737 ha, with a great number of new apple orchards with a new technology of growth. One of the most significant pests of apple is codling moth (Cydia pomonella). Good agricultural practice requires the use of products with shorter pre-harvest interval and more convenient ecotoxicological properties than previously used insecticides, such as those from neonicotionid class. In this study, acetamiprid was applied for control of C. pomonella in concentration of 0.025%, in BBCH 75 apple development phase. The field trials were carried out in the orchard near Novi Sad, Serbia. The trials were designed according to standard OEPP methods for experimental design and data analysis. In Serbia, the MRL of acetamiprid residue in apple is 0.8 mg/kg, with 28 days pre-harvest interval. Sampling was performed by random collecting from various places of the experimental plots according to the FAO/WHO recommendations. About 0.5 kg of the apple fruit was collected from each replicate. Samples were collected immediately before and after acetamiprid application (when the spraying mixture has dried), 7, 14, 21, 28 days after the application and at harvest time. The untreated apple trees were the sources of the blank apple samples, for the study of the matrix effects and to the acetamiprid recovery measurements. For the determination of acetamprid residues, the QuEChERS method extraction coupled with HPLC/DAD carried out. The method was validated in accordance with SANCO/12571/2013 criteria. The results showed that the average recovery at the four concentration levels was . ± . %; limit of quantification was established at 0.01 mg/kg; within-laboratory reproducibility of 1.59%. Matrix-effect did not significantly affect the response of acetamiprid. Content of acetamiprid in apple samples after pre-harvest interval (PHI) were below MRL. Under field conditions the half-life value of acetamprid was 0.75 days. T2-P2 ACETIC ACID IS A PROMISING POSTHARVEST TREATMENT TO IMPROVE HYGIENIC CONDITIONS, RETAIN CHARACTERISTIC QUALITY ATTRIBUTES AND PROLONG MARKETABILITY OF SWEET CHERRIES Karin Hassenberg1, Felix Schuhmann2, Nadja Förster2, Werner B. Herppich1, Martin Geyer1, Christian Ulrichs2, Susanne Huyskens-Keil2 1Leibniz-Inst. for Agricultural Engineering, Max-Eyth-Allee 100, 14469 Potsdam, Germany; zu Berlin, Lentzeallee -57, 14195 Berlin, Germany; 2Humboldt-Universität In Europe, sweet cherries are popular but highly perishable fruit revealing high postharvest quality losses due to biotic impacts and, thus, have a limited marketability. Applications of postharvest chemical (e.g. hydrogen peroxide, ethanol) and physical (e.g. heat treatment, UV irradiation) treatments that influence ripening or inhibit microorganism decay has increased worldwide. Acetic acid with its fungicidal and antimicrobial properties is registered in Germany as a preservative agent. In the present study, the impact of acetic acid on microbiological composition and fruit quality characteristics of fruit of two sweet cherry cultivars Merchant and Oktavia was investigated to extend marketability and to reduce postharvest losses. Cherries were harvested from a commercial orchard in Werder (Germany). On the same day, samples were kept untreated (controls) or subjected to three different acetic acid treatments (3 mg l-1, 6 mg l-1, 9 mg l-1), and stored at °C or °C for days. Total bacteria, yeast and mould counts, and colour, anthocyanins, sugar-acid ratio, transpiration and respiration rates, and sensory attributes were determined after 5, 10 and 14 storage days. Fruit of the two cultivars differed in their reactions to acetic acid treatments: total bacteria count, colour and anthocyanin content of Merchant samples were not significantly affected by acetic acid during storage at both temperatures. )n contrast, acetic acid treatment of Oktavia inhibited microorganisms at higher concentrations (6 mg l- and storage at °C for days. Anthocyanin content of Oktavia cherries treated 113 BOOK OF ABSTRACTS with acetic acid concentration (up to 6 mg l-1) remained constant, whereas higher concentrations tendentiously reduced colour attributes during storage. Sensory evaluation (acetic acid smell and flavour) revealed no impact of acetic acid on 'Merchant', while for 'Oktavia' samples undesired sensory attributes were obvious at acetic acid concentrations of 6 mg l-1 after 5 days of storage. T2-P3 APPLE CROP-LOAD CONTROL UNDERNEATH HAILNET Michael Zoth Kompetenzzentrum Obstbau Bodensee, Schuhmacherhof 6, 88213 Ravensburg, Germany To produce premium quality fruit, crop-load control is a necessary grower practice on orchards planted with modern apple cultivars that show abundant flower numbers as well as a high fruit setting potential. In Germany, the number of chemical compounds available for flower and/or fruitlet thinning is restricted by registration. There are commercial products to reduce fruit setting during bloom (e.g. ATS= ammoniumthiosulfate and also fruitlet thinners such as Ethephon , -benzyladenine (6-BA) or metamitron (a photosynthesis inhibitor) that are all used during the period of early fruit development (6 to 24mm fruitlet diameter up until natural fruit fall. Mechanical thinning e.g. DARW)N device undertaken during the bloom period is becoming more and more common. Combinations of early flower thinning and later fruitlet thinning treatments are now frequently used. A question often asked by apple growers is; if there is a need to use different thinning strategies in orchards with or without hail nets? In South-Western Germany, in the Lake Constance fruit growing region ~ ° North) black or white coloured hail netting is used. The photosynthetic active radiation (PAR) is reduced by ~20% underneath black netting and by ~12-15% when the trees are covered with white netting. )n the years and , three different thinning strategies for Braeburn (illwell and Pinova apple cultivars (Malus domestica Burkh.) were compared under white or black hail netting as well as on trees without any nets: ). Mechanically thinning DARW)N ; Fruit-Tec Company; Markdorf, Germany); II. ATS flower thinner + Ethephon + -BA (fruitlet thinner); III. ATS (flower thinner) +fruitlet thinning with BREV)S® ADAMA Ltd., )srael , containing the active substance metamitron. The results showed that mechanically thinning with the DARW)N device was equal to the chemical thinning treatments, and was even more effective when using the optimal machine settings (I.). Fruitset was lowest and thinning efficacy the highest with the strongest machine settings (high kinetic energy input) when compared to the metamitron photosynthesis inhibition (II.) or the ATS + 6-BA (III.) treatments. The yield parameters; number of fruit and yield (kg?) per tree and the fruit quality parameters, fruit size and blush colour were significantly better when using the DARW)N device. Comparing the chemical treatments metamitron (III.) was more effective than 6-BA (II.). Thinning efficacy was the highest underneath black netting for all the treatments, but fruit colour was reduced because of the extra shading effect, overall the amount of marketable fruit under the white netting was significantly higher compared to the black. T2-P4 ASSESSMENT OF HEAVY METAL (CADMIUM) INDUCED CHANGES IN PHYSIOLOGICAL PROCESSES OF DIFFERENT FIELD CROPS Vita Alle1, Anita Osvalde2, Uldis Kondratovics1, Mara Vikmane1 11 Jelgavas Street, LV-1004 Riga, Latvia; Street 3, LV-2169 Salaspils, Latvia; 2Miera Nowadays soil contamination with hazardous elements has become a worldwide problem. Soil contamination with heavy metals can pose a serious hazard to human health by entering the food chain. The aim of this study was to investigate the impact of increased levels of cadmium (Cd) in the substrate on plant growth, photosynthesis, chlorophyll a fluorescence and proline content with emphasis on differences between species. Vegetation experiments were carried out with barley Hordeum vulgare L. and lettuce 114 III International Symposium on Horticulture in Europe – SHE 2016 Lactuca sativa L. Plants were grown in quartz sand under controlled optimal growth conditions. Changes in physiological indices were studied at five levels of Cd: 0, 3, 6, 9, 12 mg/L for barley and 0, 1, 2, 4, 6 mg/L for lettuce in substrate. Photosynthetic pigments were determined by spectrophotometry and parameters of chlorophyll a fluorescence – by Handy PEA chlorophyll fluorometer. The proline content was determined using the ninhydrin method. The level of Cd in plant material was estimated by AAS Perkin Elmer AAnalyst 700A. Plant cultivation under increasing levels of Cd resulted in a progressive inhibition of growth. It was determined that under 6 mg/L of Cd in substrate (Cd6) the concentration of Cd in lettuce leaves was 54.64% higher than in barley leaves. The obtained results confirmed that changes in photosynthesis describing parameters are a species-specific response to Cd toxicity. Thus, under Cd6 treatment, the content of chlorophyll a+b in barley leaves was 3 mg/g, but in lettuce leaves 0.66 mg/g. A considerably decreased accumulation of free proline induced by Cd stress was found for lettuce leaves (18.5% lower than in control plants), while there was almost no effect of Cd treatment on proline concentrations in barley leaves. The changes in accumulation of the all biochemical compounds studied as a possible plant protective mechanism to Cd stress is discussed. T2-P5 CHEMICAL COMPOSITION AND QUALITY OF VARIOUS GARLIC (ALLIUM SATIVUM L.) GENOTYPES CULTIVATED IN GREECE Georgia Ntatsi 1 , Spyridon Petropoulos 2 , Angela Fernandes 3 , Lillian Barros 3 , Isabel Ferreira 3 1 Agricultural University of Athens, Laboratory of Vegetable Crops, Iera Odos 75, 11855 Athens, Greece University of Thessaly Dpt of Agriculture, Crop Production and Rural Environment, Fytokou Street, 38446 N. Ionia Magnissia, Greece 3 Mountain Research Centre C)MO, ESA, Polytechnic )nstitute of Bragança, Campus de Santa Apolónia, , -855 , Bragança, Portugal 2 Garlic (Allium sativum L.) is an important vegetable crop in Greece, with many areas being specialized in garlic cultivation through the last decades and considered as the main production areas of the crop in Greece. However, despite the significance and the high annual income of this crop there is a decreasing trend in total cultivated area, whereas the local genotypes that used to be cultivated and adapted in certain areas are gradually neglected in favour of new imported genotypes. In the present study, various garlic genotypes (Greek genotypes and imported cultivars) were assessed for their chemical composition and quality features. Samples of garlic bulbs were collected from local farmers from different regions of Greece (e.g. 2 samples of Nea Vissa garlic from Evros Prefecture, samples of Tripoli garlic from Arcadia Prefecture, sample of Psachna Euboeas garlic from Euboea Prefecture, samples of Neapoli garlic from Laconia Prefecture, and 2 samples of Rizomylos garlic from Magnissia Prefecture . All the samples were assessed for the morphology of their bulbs and individual cloves, according to International Plant Genetic Resources Institute (IPGRI) descriptors guide for Allium sp. Moreover, chemical composition and quality features (antioxidant activity, total phenolics, organic acids, dry matter content, sugars content, total soluble solids) were analyzed. From the results of the present study significant differences in morphology and quality features were observed, indicating the significance of genetic variability and the importance of preservation of genetic material for this specific crop. 115 BOOK OF ABSTRACTS T2-P6 COMPARISON OF YIELD AND SOME FRUIT QUALTY CAHARACTERISTCIS OF SOME FRESH AND FRIGO PLANTS OF STRAWBERRY VARIETIES Kader Erçik1, Nesibe Ebru Kafkas2, Murat Kaya1, Κenan çelik1, Zarifikhosroshahi3, Mehmet Ali SARIDAS4 1Diyarbak305r ozghan International Agriculture Res., Diyarnak305r, Turkey; of Horticulture , Faculty of Agriculture, TR-01330 Adana Balcali, Turkey; 3University of Cukurova, Adana, Turkey; 4University of Cukurova, Faculty of Agricult, University of Cukurova, Faculty of Agricult, University of Cukurova, Faculty of Agricult, Adana, Turkey 2Department In this study it was aimed to compared 6 various commercial strawberry varieties (Kabarla, Sweet Ann, DPI Rubygem, Festival, Amiga, Florida Fortuna) based on their fruit yield and some fruit quality characteristics in Diyarbakır region South Eastern of Turkey. The frigo and fresh plants of strawberry varieites were compared also during 2015-2016 growing year period. Average total yield (kg/da) and some important fruit quality characteristics such as fruit weight (g/plant), total soluble solid content (%), titratable acidity, fruit color, sugar/acid ratio, fruit firmnes (kg/cm2) were compared. In addition, first blooming time and first harvesting time also compared. T2-P7 DIFFERENT PRUNING SYSTEMS IN PLUM (PRUNUS DOMESTICA L.) EFFECTS ON FRUIT YIELD AND QUALITY Ayzin B. K“den, Ali K“den, Kadir Sarier, Burhanettin )mrak University of Cukurova, Dean of the Faculty of Agriculture, Department of Horticulture, 01330 Adana, Turkey. The study was carried out at the experimental area of Pozanti Agricultural Research and Application Center in Adana at 2013-2015 period (two year). Pruning has been used in ordet to controlling tree size and to maintain a balance between leaf/fruit ratio, fruit size, fruit colour and other quality parameters. Two plum cultivars (Black Daimond, Angeleno) and friar as pollinizer grafted on Myrobolan29C rootstock were used as experimental material. The trees produced commercial yields beginning in 2014, the 3rd growing season. The aim of the present study was compared four training systems (spindle, Quad-V, central leader and open vase) trees and different planting distance (80 -100-120-160-200-400cm, standard inter rows 5m) in terms of yield kg/cm and fruit qualıty factors such as fruit weight, coloration, firmness, seed weight, TSS, acidity, fruit sunburn percentage). Also, morphological, phenological observations and chill accumulation of experimental area were detected during the research. Sufficient chilling accumulation was calculated in both year for crop load. The best high density training system has been found as Spindel (120cm) in terms of all parameters for both cultivars. In other systems the best results were found respectively, central leader system (160cm), Quad V and Open Vase system (200cm). T2-P8 EFFECT OF BIOSTIMULANTS USE IN EARLY POTATO PRODUCTION IN POLAND Wanda Wadas, Tomasz Dziugiel Siedlce University, Department of Vegetable Crops, 14 B. Prusa St., 08-110 Siedlce, Poland. The growing period for early potatoes is extremely short, only ‐-80 days from planting to harvest. To obtain a highly marketable tuber yield in such a short period good conditions for plant growth must be ensured. In recent years, there has been an increase in the use of biostimulants. The effect of commercial seaweed extracts Bio-algeen S90 (Ascophyllum nodosum) and Kelpak SL (Ecklonia maxima) as well as the humic and fulvic acid mixture HumiPlant on tuber yield and quality of early crop potato was determined. The field experiment was carried out in east-central Poland ° 'N, ° 'E , with the less favourable thermal 116 III International Symposium on Horticulture in Europe – SHE 2016 and moisture conditions for potato cultivation for early crop, during three growing seasons from on Luvisol. Potatoes were harvested 75 days after planting (the end of June). ‐ , The usage of the biostimulants did not affect the tuber number per plant, whereas the increase in the tuber weight per plant was found. Applying seaweed extracts Bio‐-algeen S90 and Kelpak SL resulted in the highest total and marketable tuber yield (diameter >30 mm) increases in the year of both the highest air temperature and the largest amount of rainfall in June, while the mixture of humic and fulvic acids gave the best results in the year of the lowest air temperature and shortage rainfalls in June. The biostimulants contributed to improved marketable value of potato yield due to an increase share of medium-sized tubers (diameter 41-50 mm). The biostimulants did not affect the contents of dry matter, monosaccharide and totalsugars, L-ascorbic acid, total protein and nitrates (V) in tubers, as well as the contents of Ca, Mg and Na, however, the increase in the K content was proved. Bio-algeen S90 caused the increase in the starch content, while HumiPlant resulted in the decrease in the P content. T2-P9 EFFECT OF ELICITOR APPLICATIONS ON THE COLOR AND CHLOROPHYLL COMPOSITION OF LEAF LETTUCE (LACTUCA SATIVA L.) Nuray Akbudak1, Sevinc Basay2 1Uludag University, Fac.of Agric., Dept. Of Hortic., Gorukle Campus, Bursa 16059, Turkey; 2Karacabey Vocational School, Uludag Univer, Karacabey, Bursa, Turkey The effect of Trichoderma harzianum (T. harzianum), chitosan and methyl jasmonate treatments on chlorophyll accumulation and color, marketable yield, number of deformation leaves per plant were studied in lettuce. T. harzianum, chitosan and methyl jasmonate was applied to the lettuce (Lactuca sativa. cv. Arapsacı grown in greenhouse conditions. Changes in total chlorophyll content, colour parameters L, a, b were determined after treatments. Total chlorophyll content increased with T. harzianum, whereas no difference was found between methyl jasmonate and chitosan treatments. Lettuce in L value was determined the differences between treatment. Application to plants of T. harzianum has been determined to positively affect the values of total chlorophyll. Color values were also determined that these are in support. Values obtained from marketable yield parameter in the plants subjected to T. harzianum treatment were higher than methyl jasmonate and chitosan treatments. It was determined that the T. harzianum treatments increased the marketable yield of lettuce 12.29%. T2-P10 EFFECT OF FRUIT MATURITY, FRUIT POSITION AND NETTING ON THE DEVELOPMENT OF SOFT SCALD IN MALUS DOMESTICA 'HONEYCRISP' DURING STORAGE Ines Hanrahan 1, Stefan Roeder 1, James Mattheis 2, 1 1719 Sprinwater Ave, Wenatchee WA 98801, United States of America 2 1104 N. Western Ave., Wenatchee WA 98801, United States of America The purpose of this study was to investigate the influence of fruit position within the tree (three positions: . , . , . meters , the sequence of harvest and light environment netting by Extenday™, % reduction in radiation) on the occurrence of soft scald during storage of Honeycrisp apples. Samples were harvested three times in weekly intervals from an orchard in Gleed (Yakima County, Washington, USA) in 2015. After harvest, fruit was analyzed using standard maturity indicators and the DA-meter. The remaining fruit was stored in a commercial cold room for three months at . °C and semi-weekly measurements of the DA-index and soft scald occurrence (absent/present) were performed. 117 BOOK OF ABSTRACTS The first soft scald symptoms were observed two weeks after harvest. At the conclusion of the study, fruit with advanced maturity at harvest (third pick) showed higher incidence of soft scald (up to 79 %) than samples from the first (0 %) and second (4 - 8 %) pick. Furthermore, fruit position within the tree influenced the onset and the severity of soft scald symptoms. For example, fruit from the third pick, showed a higher soft scald occurrence (79 %) in fruit samples from the bottom than the fruit from the middle (54 - 58 %) and top (33 %) part of the canopy. There was no significant difference between the netting and control treatment. The DA-index did not correlate with the occurrence of chilling injury in this and other studies. The possibility to improve postharvest performance of Honeycrisp apples based on orchard criteria will be discussed. T2-P11 EFFECT OF NITROGEN AND PHOSPHORUS FERTILIZATION ON GROWTH COMPONENTS, YIELD AND TUBER QUALITY CHARACTERISTICS OF TWO POTATO CULTIVARS GROWN UNDER ORGANIC PRODUCTION SYSTEM Alexios Alexopoulos1, Theodore Varzakas2, Stavros Karras1, Antonia Koriki3, Anastasios Kotsiras4, Ioannis Xynias5 1Laboratory of Agronomy, Department of Agricultural Technology, Technological Educational Institute of, Peloponnese, 24100 Kalamata, Greece; 2Department of Food Technology, Technological Educational Institute of Peloponnese, 24100 Kalamata, Greece; 3Laboratory of Soil Science, Department of Agricultural Technology, Technological Educational Institute of Peloponnese, 24100 Kalamata, Greece; 4Laboratory of Vegetable Production, Department of Agricultural Technology, Technological Educational Institute of Peloponnese, 24100 Kalamata, Greece; 5Laboratory of Genetics and Breeding, Department of Agricultural Technology, Technological Educational Institute of, Western Macedonia, 53100 Florina, Greece. Fertilization requirements of potato crop depend on cultivar characteristics and they are strongly affected by environmental conditions. However, the response of potato plants to nitrogen and phosphorus fertilization is affected by fertilization technique (e.g. type of fertilizers, time of fertilizers application). We studied the effect of two levels of nitrogen = . and N = . g per plant and two levels of phosphorus P = . and P2=5.2 g P2O5 per plant) on the growth, yield and tuber quality of two potato cultivars (Voyager, Spunta), grown during spring season at Kalamata, Greece. Seed tubers were planted in 11-L pots containing a mixture 1:1 (v/v) of peat (pH=6, no additional inorganic nutrient elements) and perlite. In all plants the same amount of PatentKali (0-0-30+10%MgO+42%SO3) was applied (6.6 g K2O and 1.3 g MgO per plant). In order to meet the fertilization treatments, the fertilizers Biosol (7-0.5-0.3), Phosphorite (0-27-0) and Acadian 1-1-16 were used. In both cultivars, the higher N-dose increased the fresh weight of leaves, shoots, tubers, and shoot Ncontent. It, also, increased N-content of tubers when phosphorus was applied at the lower dose. In both cultivars, the higher P-dose increased the fresh weight of shoots, tubers N-content when nitrogen was applied at the lower dose. Phosphorus did not affect tuber yield in Spunta . On the other hand, the higher Pdose increased tuber yield in Voyager . )n addition, the higher P-dose increased leaf N-content in Voyager when nitrogen was applied at the lower dose. However, fertilization treatments did not affect dry matter content, P-content and K-content of leaves, shoots and tubers. We conclude that, in organic farming system, tuber yield is favoured by nitrogen fertilization in both Spunta and Voyager and by phosphorus fertilization in Voyager , but tuber quality characteristics are not affected by nitrogen or phosphorus fertilization. 118 III International Symposium on Horticulture in Europe – SHE 2016 T2-P12 EFFECT OF NITROGEN FERTILIZATION ON LYCOPENE CONTENT AND QUALITY TRAITS OF TOMATO (LYCOPERSICON ESCULENTUM L.) FRUITS FOR FRESH MARKET AND PROCESSING Stanislaw Kaniszewski, Ryszard Kosson, Artur Kowalski, Maria Grzegorzewska, Justyna Szwejda-Grzybowska Research Institute of Horticulture, Konstytucji 3 Maja 1_3, 96-100 Skierniewice, Poland; Two cultivars of tomatoes cv. Volna F1 for fresh market and cv. Calista F1 for processing were cultivated in the field under nitrogen fertilization rates (0, 50,100 and 200 kg N.ha-1). Fully ripen fruits from two succeeding harvests were analyzed for content of dry matter, extract, ascorbic acid, total sugars, soluble polyphenols, total flavonoids and lycopene as well as antiradical activity (DPPH). It was found that nitrogen fertilization had significant effect on yield of tomato. The average total yield for both tested cultivars was highest at the dose of 50 kg N ha-1, and marketable yield was highest at a dose of 100 kg N ha-1. Cultivar Calista F1 gave the highest total and marketable yield at a dose of 100 kg N ha-1, while in the case of cv. Volna F1 total yield was the highest at the dose of 200 kg N ha-1 while the marketable yield did not differ significantly for all nitrogen treatments. Fruits of cv. Calista F1 from second harvest were characterized with slightly higher content of lycopene and soluble polyphenols as well as higher antiradical activity compared to fruits from first harvest. The higher nitrogen fertilization during cultivation influenced on slight decrease of dry matter content in tomatoes of cv. Calista F1, as well as decrease of ascorbic acid content in fruits of both cultivars. The increase of lycopene content by about 75% in first harvest and about 85% in second harvest in tomatoes for cv. Volna F1 fertilized with highest nitrogen rate (200 kg N ha-1) in comparison with control (0 kg N ha-1) tomato was observed T2-P13 EFFECT OF SULFATE ANIONS ON NITRATES ACCUMULATION AND TOTAL KJELDAHL NITROGEN CONTENT IN LETTUCE PLANTS CULTIVATED IN PEATPERLITE MIXTURE Karipidis Charalambos, Dimitra Douma, Paraskevi Yfanti, Konstantinos Zisis, George Patakioutas Epirus Institute of Technology, Kostakioi, 47100 Arta, Greece. Intensive agriculture is characterized by increased use of synthetic nitrogen fertilizers with result the higher concentrations of vegetable and drinking water nitrate levels than in the past. Increased vegetable nitrate content is related to possible implications for health as nitrate metabolites can cause carcinogenesis, infantile methaemoglobinaemia and possibly even teratogenesis. The aim of the present study was to investigate the effect of sulfate ions on nitrate accumulation and Total Kjeldahl Nitrogen (TKN) content in lettuce (Lactuca sativa var crispa cv. Lollo Bionda) leaves as an attempt to suggest possible ways to reduce nitrate levels. Plants were grown in peat-perlite mixture fertilized by nutrient solutions under glasshouse conditions. Three levels of nitrogen concentrations (100, 200 and 400 mg L-1 N) have been applied in combination with three levels of sulfate anions (15, 150 and 300 mg L-1 S as SO42-). At harvest stage, the fresh weight of leaves and TKN tissue content was measured. Nitrate accumulation was determined spectrophotometricaly by the chromotropic acid method. As expected, foliar biomass, nitrate accumulation and TKN content was enhanced when nitrogen concentration was raised. However, the increased levels of sulfates (150 and 300 mg L-1 S) had no effect on plant weight whereas a decline of nitrate accumulation was observed when sulfate fertilization increased. It was also realized that 150 mg L-1 S caused TKN increase while 300 mg L-1 S had the opposite effect. The results indicate that at highest concentrations of sulfates, the decline of nitrate accumulation in lettuce leaves may be due to interference in the uptake of nitrates by sulfates ions and may be proposed as a possible way to decrease nitrate content in lettuce resulting in health protection. 119 BOOK OF ABSTRACTS T2-P14 EFFECT OF WOODCHIPS MULCH AND PERFORMANCE OF EIGHT BLACKCURRANT CULTIVARS Valda Laugale, Sandra Dane, Liga Lepse, Sarmite Strautina Institute of Horticulture, Latvia University of Agriculture, Graudu 1, Dobele, Latvia Blackcurrant is one of the main berry crops grown in Latvia. Local climatic conditions and market demands require an accurate choice of well adapted cultivars and improving of growing technologies in order to obtain higher yield and reducing production costs. This study presents the evaluation results of eight blackcurrant cultivars: Bagira , Ben Connan , Ben Tirran , Chereshneva , Lentyai , Mara Eglite , Veloi and Titania in two growing technologies - with woodchips mulch and without mulching. The trial was established in at P”re (orticultural Research Centre. Plant vegetative growth, phenological parameters, winter hardiness, yield, fruit quality, spreading of pests and diseases were evaluated between 2012-2015. Chereshneva was the most promising between all tested cultivars. Mulching of soil with woodchips did not influence blackcurrant phenological development, productivity and damage severity by pests, whereas it reduced weeds growth, leaf spot disease severity, increased blackcurrant berry mass and influenced vegetative growth. T2-P15 EVALUATION OF EMA PACKAGE FOR HALF, EIGHTH AND SLICES FRESH CUT OF `SPINOSO SARDO´ GLOBE ARTICHOKE Anna Barbara Pisanu1, Limbo Baghino2, Davide Sanna2, Efisio Antonio Scano3 1Agris Sardegna, V. Le Trieste 111, 09129 Cagliari, Italy; Sardegna Azienda Palloni, 09170 Oristano, Italy; 3University of Sassari, Dep. of agriculture, 07100 SS Sassari, Italy; 2Agris Several researches that use an equilibrium modified atmosphere (EMA) packaging to obtain entire artichokes minimally processed were conducted successfully. Attempts to apply the same technique to package half, eighth and slices fresh cut artichoke are in progress. The artichoke is characterized by a high respiration rate even higher when processed into eighths or slices. The aim of this research is to determinate the required gas transmission rates of the sealing film for half, eighth and slices fresh cut artichoke using in the packaging a gas mixture composed by 5% O2, 5>%CO2, N2 balance. Before processing upper thorns of artichoke are cut and harder lateral bracts pulled out. After packaging inside of each product composition of the atmosphere is monitored and when an equilibrium atmosphere is reached respiration rate will be calculated. Evaluation of the designed packaging is carried out for packages with different combination of net weights and chilled storage conditions (passive and conventional refrigeration). The shelf life of the products up to 10 days is assessed through microbiological analyses and sensory evaluations. T2-P16 EVALUATION OF HIGHBUSH BLUEBERRY CLONAL PROGENIES FOR FRUIT QUALITY Irina Ancu, 2, Monica Sturzeanu 2, Song Young un 1, Min Kyung Kwon 1 , Dong Whan Suh 1, 1 Agricultural Research and Extension Service, 189 Dongho-Dong, Buk-Gu, Daegu, 702-708, Gyeongsangbuk-Do, Korea 2 Researche Institute for Fruit Growing, Pitesti, O.P.1, C.P.73, cod 110006, Arges, Romania; Since 1998, about 540 blueberry selections coming from 12 seedlings (Vaccinium corymbosum) populations, where compared in first level of field plots, at Research Institute for Fruit Growing,Pitesti,Romania. The task to evaluate some fruit quality traits requested for the fresh market: fruit weight, fruit color, % brix, size index, firmness and flavor. After the first evaluation level, only 32 promising selection and 4 varieties passed 120 III International Symposium on Horticulture in Europe – SHE 2016 through the next level of field trials. The selection 3/85 with the highest value of mean fruit weight (3,92 g/fruit) and Safir variety recorded the highest value of fruit size index, both with more than 14 % brix were promoted for large scale propagation. An efficient selection for yield components has to be carried out for next period. T2-P17 EVALUATION OF LEGUME-CEREAL SEED MIXTURES FOR ENHANCEMENT OF SOIL FERTILITY, CARBON STORAGE AND BIODIVERSITY IN A LOW-INTENSITY OLIVE ORCHARD Omar Abo Fakher 1, Georgios Doupis 2, Androniki Papafilippaki 2, Georgios Psarras 2, Georgios Koubouris 3, 1 Mediterranean Agronomic Institute of Chania, Alsyllio Agrokepiou, Chania, Greece 2 Institute for Olive Tree, Subtropical Crops, 73100 Chania, Greece 3 Olive Cultivation Laboratory, Institute for Olive Tree and Subtropical Pl, ELGO DEMETER, (NAGREF), 73100 Chania, Greece The main aim of the LIFE+ OLIVECLIMA project is to trial the introduction of new cultivation practices for tree crops in order to find a cost-effective means of mitigating and adapting to climate change, through the increase of carbon sequestration by soils, and reduction of greenhouse gases emissions. In order to determine the benefits of using cover crops on soil fertility and biodiversity, an investigation with different combinations of legumes and oat has been conducted during 2014-2015 in Chania, Greece in a low tree density rain-fed olive orchard covered dominantly by Oxalis pes-caprae weed. The treatments were: natural vegetation, bare soil, only oats, only vicia sativa, mixture of four legumes with oats and mixture of five legumes with oats. Seed emergence and soil cover was monitored and soil was monthly sampled at three depths (0-10, 10-20 and 20-30 cm) for analysis. During plant growth, significant fluctuations in soil NO3- and NH4+ content was recorded. Similarly, soil CO2 emissions increased with plant growth and air temperature raise. During the first six months of the trial, no significant differences were observed in soil organic matter, pH, EC, P and K content. Increased content in plant tissues (roots) was recorded in intact soil samples indicating carbon storage and potential for future increase in soil organic matter through their decomposition. Sowing cover crop mixtures is a promising soil management practice to increase soil fertility and enrich plant and microbial biodiversity in olive orchards especially when dominated by Oxalis pescaprae a very aggressive weed in many Mediterranean areas. Long-term monitoring of soil agro-ecosystem functions is necessary to determine further implications and sustainability of this practice in the frame of overall orchard management. T2-P18 EVALUATION OF THE POSTHARVEST QUALITY INDICATORS OF SOME STRAWBERRY CVS Monica Sturzeanu, Mihail Coman, Irina Ancu Research Institute for Fruit Growing, OP 1, CP 73, Pitesti, Romania; The objective of the study was to evaluate some postharvest parameters of fruit quality at seven strawberry cvs., ('Alba', 'Clery', 'Coral', 'Magic', 'Premial', 'Queen Elisa', 'Record') grown at RIFG Pitesti, Romania in open field, in 2012-2014 period. The storage method included three days in normal refrigeration condition (2- ºC and one day at room temperature (22- ºC . The following parameters were determined before and after storage: fruit weight g , fruit firmness kgf/cm , four color indicators L*, a*, b*, ∆E and percent of damaged fruits caused by pathogen.Statistically assured differences were recorded between cultivars. 'Premial' cv. proved the lowest fruit weight loss (0,27 g) and also the lowest fruit firmness loss (0,09 kgf/cm2) after storage. 'Alba' cv. had highest percentage of healthy fruits after storage (86,4%). After four days storage both early cvs. were marketable. 121 BOOK OF ABSTRACTS T2-P19 FRUIT QUALITY AND YIELD OF STRAWBERRIES AS AFFECTED BY SOIL AND FOLIAR IODINE FERTILIZATION Diemo Daum1, Christian Meinecke1, Christoph Budke1, Patrick G. Lawson2, Ketut A. Wijaya3 1University of Applied Sciences Osnabrueck, Faculty AL, Oldenbruger Landstr. 24, 49090 Osnabrueck, Germany; 2University of Applied Sciences Osnabrueck, Emsweg 3, 49090 Osnabrueck, Germany; 3Faculty of Agriculture, University of Jember, Jl. Kalimantan 37, 68121 Jember, Indonesia; The iodine content of food crops is usually very low and thus their edible parts contribute little to the iodine intake in human nutrition. Hence, a balanced diet rich in vegetables and fruits often does not ensure an adequate alimentary iodine supply. Although not essential for their growth, higher plants are principally able to absorb iodine by roots and leaves when it is exogenously applied. In this study we investigated the influence of soil- and foliar-fertilized iodine on fruit quality and yield of strawberries in open cultivation. The trials were conducted with two strawberry varieties, ʹElsantaʹ and ʹSenga Senganaʹ which were cultivated on a sandy loam soil in the first and third cropping year, respectively. Iodine was applied by single KIO3 soil drenches two weeks after planting or, alternatively, by using KI foliar sprays from the beginning of flowering. The experiments were arranged as a randomized block design with 4 replications. Soil fertilization of KIO3 resulted in a relatively low iodine accumulation in strawberry fruits (maximum 12 µg ) per g FM , most probably, because the content of iodine in the soil rapidly decreased after its application. In contrast, the targeted iodine content in fruits was achieved when KI was aerially applied, either by a single treatment shortly before harvest or by repeated sprays during the flowering period (up to µg ) per g FM . Αield, flesh firmness and total acidity content of strawberry fruits were not affected by any of the tested iodine applications. However, as a result of repeated foliar sprays the sugar content of the fruits was slightly diminished and the colour of leaves turned to reddish-violet. On the other hand, infection of botrytis on strawberry plants cultivated in the third season was significantly reduced by repeated foliar iodine treatments. T2-P20 GROWTH AND ANTHOCYANIN ACCUMULATION OF PURPLE CARROTS IS IMPACTED BY HARVESTING DATE, BUT NOT BY REDUCED NITROGEN SUPPLY Lilian Schmidt, Sophia Sorg, Susanne Tittmann, Johannes Max, Jana Zinkernagel Geisenheim University, Department of Vegetable Crops, Von-Lade-Str.1, 65366 Geisenheim, Germany Purple carrots contain high amounts of anthocyanins which is interesting for the production of natural dyes. It is therefore of relevance to increase the anthocyanin concentration and the yield by agricultural practices. For example, limited nitrogen (N) supply is known to enhance anthocyanin concentrations in variable plant species. Moreover, extending the harvesting period may have beneficial effects on anthocyanin accumulation in carrot roots. This was tested by growing the purple carrot variety `Deep Purple´ Bejo Samen Gmb(, Germany) in sandy soil in May 2015. The total N supply was 220 kg N ha-1 for control and 33% of the optimal recommended value (=73 kg N ha-1) for the N-limited treatment. Harvests were conducted in late September, late October and late November 2015, respectively. Upon harvest, roots were classified according to their diameter. On randomly selected plants, N, anthocyanin and chlorophyll concentrations in leaves and roots were assessed by spectroscopic measurements (Multiplex 3 Research and Dualex Scientific +, both Force-A, France). Additionally, chlorophylls were extracted in acetone and anthocyanins in methanol followed by quantification using photometric measurements. The carbon and N status was determined by combustion. N reduction did not impact any parameter. However, later harvests had positive effects on yield, root diameter and the spectroscopic values describing the anthocyanin concentration in the roots. In leaves, the anthocyanin concentration increased with time while chlorophyll was degraded. Senescence thus was not detrimental for anthocyanin production and secondary growth. Extending the harvesting period can be 122 III International Symposium on Horticulture in Europe – SHE 2016 used to achieve higher yields and higher concentrations of anthocyanins. Moreover, N supply may be reduced to 33% of the recommended values for the variety `Deep Purple´. T2-P21 HOW CLIMATE CHANGE AND LACK OF CHILLING MAY AFFECT FRUIT GROWING IN A FUTURE WITH WARMER WINTERS Heiko Kaufmann1, Michael Blanke2, Achim Kunz3, Eike Luedeling4 1INRES- Horticultural Science, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany; Institut fuer Obstbau Bonn, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany; 3CKA Klein-Altendorf, University of Bonn, Meckenheimer Str. 42, D-53359 Rheinbach, Germany; 2INRES- 4ICRAF, Dept of Agroforestry, Nairobi, Kenya. Sweet cherry (Prunus avium L) and apple (Malus domestica Borkh.) trees require 400-1,600 chilling hours depending on the variety and the location. Climate change and global warming poses a major problem in cherry and apple growing worldwide, because insufficient chilling during the winter months can disrupt flowering of fruit trees. Indigenous fruit trees have adapted to the local weather conditions over a long period of time. With changing weather conditions, also due to the continuously faster advancing climate change, warm winter can lead to problems in the bloom of high chill fruit trees such as sweet cherries and apple with a high chilling demand in winter. In the valleys of the Cape region in South Africa, in Morocco and in the Provence (France), certain high chill sweet cherries are no longer cultivated. A worldwide survey of chilling development in the future has identified an increase in chilling in the Scandinavia and Sibiria, whereas a dramatic loss of chilling is predicted for the Mediterranean countries. Orchardists need to know the chilling needs of their fruit trees to plant just for the regional temperature conditions adapted varieties to counteract possible failures. Countermeasures include breeding, defoliation in the autumn, evaporative cooling and shading during winter,dormancy breaking agents and moving to higher elevation. The results of the four-year-study at Klein-Altendorf University of Bonn, Germany °N using potted sweet cherry trees with three different chilling requirements (500-1,500 CH) under natural diurnal light and temperature conditions showed that the dynamic model appears most suited in describing chilling requirements in sweet cherry at this location. The presentation will discuss various adaption strategies to combat adverse effects of climate change, but also pinpoint at its challenges and opportunities. T2-P22 IMPACT OF HIGH CO2 ON RIPENING BEHAVIOUR AND DISCOLORATION OF BANANA FRUIT (MUSA X PARADISIACA) Werner B. Herppich1, Anja-Magret Mink2, Guido Rux1, Thanos Papageorgiou3, Martin Geyer1, Christian Ulrichs2, Susanne Huyskens-Keil2 1Leibniz-Inst. Agricult. Eng. Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany; 2Lentzeallee 5557, 14195 Berlin, Germany; 3Banksstrae 28, 20097 Hamburg, Germany; Banana Musa x paradisiaca Cavendish is one of the most important import fruit in Europe. Export countries, e.g. Columbia and Costa Rica, commercially harvest fruit at a mature green stage and transport to e.g., Rotterdam or Hamburg. Upon arrival at harbours, initiation of fruit ripening processes via ethylene treatment is performed in ripening chambers. Recently, fruit quality problems emerged during ripening comprising peel discoloration of unripe fingers associated with an irregular ripening behaviour and undesired sensory. It was reported that high CO2- and/or low O2-concentrations might cause peel browning 123 BOOK OF ABSTRACTS and/or off-flavour in banana fruit (Kader 2002). In the present study, the impact of high CO2- and low O2concentrations on peel discoloration, ripening behaviour and sensory quality of Cavendish banana was investigated. `Cavendish banana origin Colombia arrived at (amburg harbour, and on the same day, fruit were stored at . °C for d under different CA conditions: 1) 1% O2/5% CO2; 2) 1% O2/12% CO2; 3) 3% O2/12% CO2; % O / % CO . Ripening was initiated by ethylene treatment at °C for d with batches a directly at time of arrival b after d, or c after d. Control samples were stored at . °C under ambient atmospheric conditions. Determination of gaseous compounds and evaluation of external quality (colour, peel browning), sugar-acid-ratio, starch and sensory attributes were determined after 8 and 15 d of storage. Results of the various experiments demonstrated that high CO2-concentrations did not contribute significantly to peel discoloration and disturbance of the ripening process. This is in contrast to findings reported earlier and will be discussed in detail. However, a non-completed yellowing was observed in fruit under low O2-concentrations, whereas sensory attributes were not negatively affected by any of the CA treatments. T2-P23 IMPACT OF POSTHARVEST CHLORINE DIOXIDE TREATMENT ON MICROBIAL DECAY AND PHYSIOLOGICAL QUALITY OF WHITE ASPARAGUS (ASPARAGUS OFFICINALIS L.) Karin Hassenberg1, Werner B. Herppich1, Susanne Huyskens-Keil2 1Leibniz-Inst. for Agricultural Engineering , Max-Eyth-Allee 100, 14469 Potsdam, Germany; zu Berlin, Lentzeallee 5557, 14195 Berlin, Germany. 2Humboldt-Universität White asparagus (Asparagus officinalis L.) is a very important crop in Germany, which is now increasingly offered as a fresh convenience product. Consequently, postharvest quality assurance has to focus on retardation of metabolic processes, and on recent hygienic requirements and food safety regulations. Because losses due to microbiological spoilage can be up to 30%, optimization of postharvest treatments is essential for the food supply chain management of asparagus. This investigation aimed to evaluate the effect of chlorine dioxide ClO washing water on microbial load and physiological properties of white Gijnlim asparagus spears during simulated shelf-life. In four independent experiments during four growing seasons, freshly harvested spears were washed, sorted and randomly separated into batches. Respective batches were subjected to ClO2 (5 ppm, 30 s or 50 ppm, 30 or s and tap water controls , and stored for up to four days at °C. Total aerobic mesophilic bacterial counts (TAMBC), yeast and mould counts, and physiological and textural quality parameters of spears were analysed at harvest, and on storage days 2 and 4. Odour and taste of treated and control samples were evaluated. TAMBC of treated spears (c(ClO2) = 5 ppm, t = 30 s) was one log lower than that of controls after storage. Increasing ClO2 concentrations and extensions of treatment did not further reduce TAMBC. No effect on yeasts was observed irrespective of the treatment; mould counts of controls were one log higher than at harvest. Only washing with ClO2 (c = 50 ppm, t = 30 s) resulted in 0.5 log lower mould counts; extending treatments to 90 s reduced moulds by another 0.5 log. Vitamin C content, respiration rate, dry matter, and odour and taste were not affected by ClO2-treatments. Therefore, ClO2 may be an appropriate sanitizer to minimize microbial load on asparagus spears. T2-P24 IMPACT OF PRE- AND POSTHARVEST FACTORS ON STORAGE QUALITY FOR LAMB'S LETTUCE (VALERIANELLA LOCUSTA L.) 124 III International Symposium on Horticulture in Europe – SHE 2016 AN DECOMBEL1, BERT JACOBS2, JORIS VAN LOMMEL3, ISABEL VANDEVELDE3, BERT VERLINDEN2, PETER BLEYAERT1 1 Inagro, Ieperseweg 87, 8800 Rumbeke, Belgium 2 Willem de Croylaan 42, 3001 Heverlee, Belgium 3 Duffelsesteenweg 101, 2860 Sint-Katelijne-Waver, Belgium The popularity of lamb s lettuce Valerianella locusta L. in Belgium has grown in the last years, partially due to the usage in ready to eat salad mixtures. The present study evaluated which factors do have any influence on lamb s lettuce quality in the postharvest chain. Experiments in greenhouse conditions showed a minor impact on storage quality of cultural methods. The amount of irrigation water, level of nitrogen fertilization and crop protection, heating temperature and cultivars all showed limited or no effects on the storage potential. On the other hand, considerable impact on storage quality was observed from harvest conditions and storage conditions. More precisely, influencing variables were placement of plants in packaging boxes, storage room quality, cooling temperature, and the wrapping of boxes with shrink-film. )n optimal conditions, lamb s lettuce could be stored for more than three weeks, while still fulfilling quality requirements of the vegetable markets. After two weeks storage, it was possible to revitalize withered plants by submersion in water. The most limiting factor for lamb s lettuce storage is leaf yellowing. This physiological process is hastened by higher storage temperatures and by storage time. Under good storage conditions, leaf yellowing starts to become problematic after three weeks. However, well-stored lamb s lettuce delivered to the vegetable markets just before the appearance of yellow leaves will, when entered in the distribution chain, show a fast quality loss. Therefore, as an assurance for quality preservation, a storage time of more than 10 days is discouraged. T2-P25 INFLUENCE OF NITROGEN AND SULFUR FERTILIZATION ON THE YIELD AND QUALITY OF GARLIC Priit Põldma 1, Ulvi Moor 1, Tonu Tonutare 2, Marge Starast 1 1 Estonian University of Life Sciences, Department of Horticulture, Kreutzwaldi 1, 51014 Tartu, Estonia 2 Estonian University of Life Sciences, Dep. of Soil Science and Agrochemistr, Tartu, Estonia The aim of the present research was to study the influence of top dressing with different levels of nitrogen and sulfur on the yield and quality of garlic. The experiment was carried out in 2014 in eastern Estonia ° . N; ° . E on a glossic hapludalf soil. Garlic cv. Βiemiai cloves were planted in the first week of October 2013 with plant distances within the row of 12 cm and between the rows of 70 cm. Basic fertilization was performed at the end of March with NPK fertilizer (50 kg/ha N; 21 kg/ha P; 80 kg/ha K, 45 kg/ha S) and followed by top dressing at the beginning of May with fertilizers containing different levels of sulfur. Top dressing was calculated to add 60 kg/ha of nitrogen. The total amount of applied nitrogen and sulfur was: 1. N50 S45; 2. N110 S45; 3. N110 S75 and 4. N110 S115. No irrigation was used in experimental field. Garlic bulbs were harvested on July 23. For determination of yield characteristics bulb weight and yield structure, content of total N, P, K, Ca, Mg, and S, ascorbic acid content (AAC), pungency and total phenolics were determined. The total yield ranged from 663 to 860 g/m2. Lowest yield was obtained from plots were no additional top dressing was added (N50 S45). Top dressing with high sulfur level (N110 S115) decreased total yield compared to N110 S45 and N110 S75. Treatment N110 S45 had significantly more bulbs with the largest diameter (over 60 mm) compared to the other treatments. Additional sulfur increased the pungency and content of S, allicin and AAC. Higher levels of S decreased total K and Mg content, while had no influence on P and Ca. Our results indicate that it is not necessary to use sulfur containing fertilizers for top dressing on the assumption that basic fertilizers contain sufficient level of S. 125 BOOK OF ABSTRACTS T2-P26 INFLUENCE OF NITROGEN FORM AND CONCENTRATION ON YIELD AND QUALITY OF POT GROWN BASIL Diemo Daum1, Christian Frerichs1, Robert Koch2 1University 2State of Applied Sciences Osnabrueck, Faculty AL, Oldenbruger Landstr. 24, 49090 Osnabrueck, Germany; Horticultural College and Research, Institute Heidelberg, Diebsweg 2, 69123 Heidelberg, Germany; In the organic production of pot grown basil yield depressions and quality impairments are often observed. During the early development stage cotyledons become chlorotic and necrotic. Subsequently, fungal diseases such as botrytis occur. One possible reason for this problem could be the high concentration of ammonium in the growing media released by the mineralization of organic fertilizers. Therefore, a fertilization trial was carried out to investigate the effect of ammonium on basil in comparison to nitrate. The experiment included different ammonium/nitrate ratios (100/0; 50/50; 0/100) and nitrogen concentrations in the nutrient solution (8, 12 and 16 mmol N/L). Plants were cultivated in a peat substrate and fertigated with a nutrient solution containing, beside the different nitrogen sources, equal concentrations of a base fertilizer as well as the nitrification inhibitor DMPP. In addition, an organic fertilization treatment was realized. Basil fertilized solely with ammonium showed a diminished growth in comparison to well-developed plants receiving nitrate as nitrogen source. Germination rate, plant height and fresh matter yield of herbs were significantly reduced by ammonium nutrition. Similar results occur in the organic treatment where the ammonium concentration rose up to 350 mg/L substrate at the beginning of the cultivation period. Beside a reduction in biomass production, chlorotic cotyledons were observed. These effects might have been caused by ammonium itself as well as by phytotoxic ammonia or nitrite concentrations. When nitrogen mineralization declined and ammonium was largely converted to nitrate, plants exhibited improved growth. Within the mineral nitrogen treatments, rising nitrate concentration and nitrate/ammonium ratio promoted plant height and reduced plant compactness due to an increased internode stretching. At the end of the experiment the nitrate content in basil shoots was highest in the organic treatment and lowest with ammonium as the sole nitrogen source. The best herb quality in terms of plant compactness, leaf colour (measured by SPAD chlorophyll meter), turgidity and vitality was observed when basil was fertilized with ammonium nitrate. T2-P27 INFLUENCE OF SEED PRE-TREATMENTS ON SEED GERMINATION AND EARLY SEEDLING DEVELOPMENTS OF JUJUBE (ZIZIPHUS JUJUBA MILL.) Sadiye Gozlekci1, Nafiye Adak2, Ilhami Tozlu3 1Department of Horticulture, Faculty of Agriculture, Akdeniz University, 07058 Antalya, Turkey; University, Technical Sciences Voc, Antalya, Turkey; nafiye@akdeniz.edu.tr 3Akdeniz University, Faculty of Agriculture, Antalya, Turkey; itozlu@gmail.com 2Akdeniz The Chinese jujube (Ziziphus jujuba Mill.) is among one of the oldest cultivated and utilized cultivar in fruit domestication history of China (7000 years). The Chinese jujube is still an underutilized fruit grown in natural flora of Turkey. Until recently, The Chinese jujube was propagated primarily by root suckers, but more farmers in China now grafting or budding onto seedling rootstocks to maintain benefits of elite varieties. Even if jujube seeds are viable they are not able to germinate readily due to its hard and thick woody endocarp seed coat and dormancy. Thus, best conditions for seed germination that will yield in viable and well developed seedling should be determined. This study was conducted to do so. In the research, the effects of various pre-treatments on jujube seeds native to Mediterranean region of Turkey were studied to find out best treatment for seed germination and early seedling development by means of plant heights, numbers of developed leaves, stem diameter and chlorophyll index values. Seeds, prior to sowing, were treated as follows: Control C , water treatment in hours W+ h , hot water in oC in ½ hour (W+ / h , acid treatment in for minutes ½ hour then in water for 24 hours (H2SO4 +24 h W) (A), 126 III International Symposium on Horticulture in Europe – SHE 2016 5) scarification (S), 6) dehulled seeds (D). Pre-treated seeds were seeded in 70% peat + 15% perlite + 15% vermiculite (v:v:v) media that was kept in acclimatization room at 70-75% relative humidity and 25 oC temperature. Results indicated that the highest germination rate obtained by treatment E (dehulled seeds; 63.33%) and followed by treatment S (scarification; 50.00%). The lowest germination was obtained from C (control; 3.33%). The other measurements on plant heights, numbers of developed leaves, stem diameter and chlorophyll index values were also found higher in treatments D and S. T2-P28 LEAF GAS EXCHANGE CHARACTERISTICS AND LEAF WATER POTENTIAL OF DROUGHT STRESSED LINDEN (TILIA SP.) TREES K|roly (rotkó, M|rk Steiner, M|té Vértesy Szent )stv|n University, Faculty of (orticultural Science, Department of Floriculture and Dendrology, Budapest, (ungary; The daily course of leaf gas exchange was investigated on four linden cultivars from Tilia cordata Mill., T. tomentosa Moench and T. americana L. in the summer season. On the days of measurements leaf samples for water potential measurements were also collected in early morning and during midday hours. After the morning peak rapid stomatal closure was detected on leaves of T. cordata Savaria , and Greenspire . At the beginning of summer stomatal conductance on leaves of T. tomentosa Szeleste was the highest, while in the middle of summer the highest was on T. americana Redmond . The transpiration resulted significant differences in daily water use on every days of measurements early summer: T. cordata Greenspire : . kg·m− ; T. tomentosa Szeleste , . kg·m− , middle of summer: T. cordata Savaria : . kg·m− ; T. americana Redmond : . kg·m− , late summer: T. cordata Greenspire : . kg·m− ; T. americana Redmond : . kg·m− of leaf area . Due to rapid stomatal closure T. cordata cultivars realized water saving on the cost of strongly reduced carbon gain. The highest water potential values were detected on T. cordata cultivars on every measuring day. The lowest rates were given by T. tomentosa Szeleste unless the middle of summer, when significant distinctions was not able to shown. Water potential values differed from each other notably in early morning and in midday in every investigated day. However, it was not differences in the middle of summer. The large cultivar differences in the performance of leaf gas exchange and water potential should be considered at evaluation of drought stress adaptability and environmental benefits (CO2 fixation, O2 and vapor release) of Tilia cultivars. T2-P29 PRUNUS ROOTSTOCK TESTING FOR PEACH TOLERANCE TO PSEUDOMONAS AND ARMILLARIA DISEASES IN SOUTH CAROLINA Gregory L. Reighard1, W. Greg Henderson2, David Ouellette3 1Dept. of Plant and Environmental Sciences, 161 Poole Agricultural Center, Box 340310, Clemson, SC 29634-0310, United States of America; 2304 Gray Street, Edgefield SC 29824, United States of America; 3143 Poole Agricultural Center, Clemson SC 29634, United States of America. New commercial rootstocks for peach include P. persica seedlings as well as many Prunus interspecific hybrids and plum species. However, no rootstock has been successful in replant soils having the Peach Tree Short Life Syndrome (PTSL) that includes both Pseudomonas syringae canker and Armillaria spp. root rot. Four rootstock trials consisting anywhere from 5 to 16 of 18 total rootstocks budded with 4 scion cultivars, Flameprince , Crimson Lady , Julyprince and Fireprince were planted , , , , respectively in either Edgefield or Oconee Counties of South Carolina, USA. Tree death from bacterial canker (P. syringae) occurred on all sites planted before 2015. Trees on hybrid or plum species rootstocks except MP-29 had the most mortality. P. munsoniana selections 1, 2, 3, and 4 had tree death (24-63%) from either incompatibility or bacterial canker in the 2012 test. Trees on these rootstocks were also ~40 to 50% smaller than the controls after years. (ybrid rootstocks Replantpac , Tempropac , Densipac and Purplepac had PTSL-related mortality of , , , and percent, respectively for the Crimson Lady planting. 127 BOOK OF ABSTRACTS Several Julyprince trees on Densipac and Tempropac also have died from PTSL. The plum hybrid MPhas had high survival and a semi-dwarfing effect (~50% standard) on the 2 test sites it was planted at in . Lovell and Guardian® have had minimal PTSL death from bacterial canker but Armillaria has started to kill trees in the 2012 planting. Another 3-5 years will be necessary to determine Armillaria tolerance, but the new rootstocks appear to have less resistance to bacterial canker than the commercial standards. T2-P30 RELATIONSHIPS AMONG THE ROOTSTOCK AND MORPHOGENETIC BEHAVIOUR OF APPLE TREE IN ASPECT FOR FRUIT QUALITY Giedre Samuoliene, Alina Viskeliene, Ramunas Sirtautas, Darius Kviklys Kaunas str. 30, LT-54333 Babtai, Lithuania. The relationships among rootstock and some physiological (yield, fruit quality) and physical (endogenous hormones, leaf mineral composition) indices and their effect on fruit quality were evaluated. Commercial important apple cultivar – 'Ligol' was grafted on semi - dwarf rootstock M.26, dwarf rootstock – M.9 and super-dwarf rootstock P 22. Crop load was adjusted to 60, 105 and 150 inflorescences per tree. According to results, contents of endogenous leaf hormones were not depended neither on rootstock nor on crop load, except of ABA in July. Whereas amounts of zeatin, JA, ABA, GA3 and GA1 were significantly controlled by the crop load in September. While bud hormones were both rootstock and crop load controlled. M.9 rootstock conditioned a significant increase in zeatin and ABA content in September. P, K, Ca, Mg and Zn all showed 0.4 – 5.9, Mn 1.6 – 3.7 times greater differences than optimum contents. The optimum amounts of Fe were found in all treatments. Amounts of K, Mn and Zn were rootstock dependent, changes in P, Mg and Fe were effected by M.9 and P 22 rootstocks, whereas N and Ca did not change. Crop load effected all mineral contents, except of Ca. Rootstock significantly effected fruit weight and fruit maturity time. Whereas crop load significantly effected all fruit quality characters except starch index and soluble solid content. The biggest fruits (250 g) were on M.9, the smallest (214 g) and more matured on P 22 rootstock. Fruits were smaller and more firm at 150 crop level. Apples ripened faster and blush increased at lower crop load. Generally, the delivery of zeatin, ABR, GR7, GR3 and GR1 decreased; JR - increased in all treatments throughout the season. Whereas an increase of IAR throughout July into August had stimulatory effect on shoot elongation of semi-dwarf rootstock M.26, except of the most intensive crop load. The trees on P 22 rootstock were found to have the lowest values than other rootstocks in terms of yields, fruit weight and fruit quality. T2-P31 RESEARCH ON THE INFLUENCE OF CARBON DIOXIDE-RICH ATMOSPHERE OF SHORT TERM STORAGE OF BLUEBERRY Daniela Veringa 1, Marian Vintila 2, Angela Mohora 2 1 No. 1A, Intrarea Binelui Street, District 4, 042159 Bucharest, Romania 2 Intrarea Binelui, 1A, sector 4, Bucharest, Romania A number of previous studies have established that the transportation and storage of fruit at low temperatures is insufficient in many cases and has to ensure optimal part of the gaseous medium characterized by certain concentrations of oxygen, carbon dioxide and nitrogen. The research shows the results obtained from the fruit of the blueberry maintained in a modified atmosphere of carbon dioxide at various concentrations and for different periods of maintenance. We intended to correlate the administration of carbon dioxide with the exposure time to this treatment so that by alternating carbon dioxide – rich atmosphere to reduce losses. After conducting experiments we established that all modified atmospheres with carbon dioxide have reduced losses. The high content in carbon dioxide of the storage environment exerts a direct influence of inhibition on developing pathogens 128 III International Symposium on Horticulture in Europe – SHE 2016 and carbon dioxide concentrations do not produce in fruits the essential chemical transformation that can influence their quality in a negative way. The total losses were minimal for the variant when it has been used CO2 concentration of 20%, both at hot and at cold. T2-P32 RESIDUE ANALYSIS AND DISSIPATION OF THE FUNGICIDE BOSCALID IN TABLE GRAPES, WINE AND DISTILLATES Christos Alexoudis, Dimitios Makaridis, Theodoros Doulaveris, Sotirios Papadopoulos, Zoe Adamidou, Zisis Vryzas Pantazidou 193, 68200 Orestiada, Greece. A simple and accurate analytical method for the analysis of boscalid in table grapes, wine and distillates was developed, validated and employed for the analysis of samples from vineyards grown according to integrated pest management (IPM) and conversional strategies. The IPM strategies were recorded and supervised on two table grape varieties (Crimson and Thompson seedless) and one indigenous wine grape (Roditis). Conventional plant protection programs were also applied to the above mentioned varieties. Grape samples were collected and analyzed 30 min, 2, 8, 16, 24, 28 and 36 days after the boscalid application. Moreover must, wine and spirit samples produced from Thompson and Roditis were analyzed. Grape and spirit analysis was based on an ethyl acetate extraction technique and fermentation products (must and wine) were analyzed by solid phase extraction. The analytical method showed recoveries ranged from 69 to 110%. Boscalid was extracted and quantified from all substrates with RSD < 13%. The limits of quantification (LOQ) were . μg/g, . and , μg/ml for grapes, wine and spirits, respectively. Boscalid concentrations ranged from 18.9 to 11.2 ppm 30 min after its application, in all grape samples. Thirty six days after its application, boscalid concentrations reduced to 6.1- . μg/g. Boscalid was rapidly dissipated in Thompson followed by Roditis and was more persistent in Crimson. Boscalid concentration decreased during fermedation (must and wine). However, boscalid seems to be accumulated in cake, lees and spirits. T2-P33 ROOTSTOCKS AND GRAPES CULTIVARS RESPONSE TO AN INCREASING SOIL WATER DEFICIT IN THE NORTHERN PART OF MEXICO Abelardo Nuñez-Barrios, Ramona Perez-Leal, Pasquale Cirigliano Facultad de Ciencias Agrotecnologicas, campus 1, Chihuahua, Chihuahua, 31350, Mexico In Northern Mexico, the state of Chihuahua is a major producer of fruit of temperate climate such as pecans, apples and peaches mostly growing under irrigated conditions. However the use of water is highly inefficient and water extraction for irrigation is already reaching depths below 600 m. This problem raises the need to work with more effective irrigation management strategies as well as selecting crops with less water requirements such as vine. The vine is becoming an alternative crop in Chihuahua State, especially for industrial use and wine production. The objective of this study was to understand the response of varieties and rootstock to different levels of water deficit under the environmental conditions of northern Mexico. Two contrasting varieties and two root stocks were selected for this study. Chardonnay CL18, having a lowmoderate vigor and early maturity. Merlot CL15, having a high vigor and later maturity. The root stocks were also contrasting having 3309-C as less resistant to water stress and 110R more adapted to drought conditions. Three drip irrigation treatments were applied in this experiment: 1. Full irrigation, 2. -30% of full irrigation and 3. - 60% of full irrigation. Measurements were carried on rainfall and temperatures patterns on site, plant phenology, vegetative growth, SPAD readings, Leaf temperature, nutrients uptake and grape quality. Merlot CL15 has a 20 days later maturity than Chardonnay CL18. Merlot CL15 on 110R had a greater vegetative growth on the three level of irrigation. Grape yield diminished with increased drought stress imposed on this study; however fruit quality for wine production was better under the stress treatments. 129 BOOK OF ABSTRACTS T2-P34 STRAWBERRY YIELD AND QUALITY IN INTERCROP WITH LEGUMES Sandra Dane1, Valda Laugale1, Dace Sterne2 1Abavas 2Liela iela 2, LV-3124 Pure, Latvia; iela 2, LV-3001 Jelgava, Latvia. Human health improvement has been goal for many decades. More and more people are interested in healthier food and lifestyle. Improved health can not be obtained without healthy and balanced diet. Fruits and vegetables are important part of it. One of the most popular fruit is strawberry. It is popular not only because of its looks, taste and beneficial effect on health but also for its ability to adapt to many kind of weather conditions allowing it to grow worldwide. There are many technologies to grow strawberries. Main goal of them is to maintain and increase strawberry yield and quality at the same time reducing investment of money, time and labour. In this study strawberry intercrop with different legumes was evaluated. Experiment was carried out at Pure Horticultural Research Centre, Latvia in 2015. Evaluation of strawberry cultivar Polka yield and quality was done. Local hybrid of broad bean, peas Ambrosia and Capella , clover Namejs were used as intercrop and compared to strawberries grown without intercrop. There were not stated significant differences within strawberry marketable yield, unmarketable yield and Botrytis cinerea damaged berries between growing variants in 2015. Concluding from one year data intercropping strawberries with legumes can give the same yield and quality strawberries as in traditional growing system. T2-P35 THE CROP OF GRAFTED PEPPERS AN ALTERNATIVE - CONCERNING THE YIELD AND QUALITY - FOR VEGETABLES GROWERS Marian Bogoescu 1 Bucharest, Intrarea Binelui, No.1A, Sector 4, Cod. Postal 042159, Romania Soil fumigation has been an essential component of greenhouses crops since the s. Growing vegetables without soil fumigants has remained a challenge, in part because commercially acceptable pepper cultivars produced through conventional breeding lack resistance to many soil borne plant pathogens. In field production, crop rotation is important to prevent infestation of diseases and pests. However, crop rotation is rarely practiced in greenhouse production, which allows soil borne pathogens and pests to accumulate, progressively reducing crop yields and fruits quality. Grafting cultivars with high quality and productivity on rootstocks that are resistant to the soil pests and diseases is a method known from years ago, which was improved and quickly spread in the last years. The aim of this research was to evaluate the performance of the grafted peppers on some rootstocks, in greenhouse conditions. Marketable yield, fruits quality and nutritional qualities (total soluble solids contents, dry matter, titratable acidity, PH , soluble carbohydrates, Vitamin C content) have been determined. The marketable yield and fruit number were positively influenced by rootstock compared with ungrafted peppers. The obtained results showed that grafting has improved the commercial quality of pepper fruits. There were no significant differences on the nutritional qualities of fruits peppers coming from the grafted or ungrafted plants. T2-P36 THE EFFECT OF NITROGEN FERTILIZATION AND POSTHARVEST TREATMENT WITH 1-MCP AND ETHYLENE ON DURABILITY OF TOMATO FRUITS Maria Grzegorzewska, Stanislaw Kaniszewski, Ryszard Kosson, Ewa Badelek, Kalina Sikorska-Zimny Research Institute of Horticulture, 96-100 Skierniewice, Poland. The study was carried out on the tomato cv. Calista F1 and Volna F1. Tomatoes for the storage were harvested in full red color. There was evaluated the storage ability of tomato, fertilized during growing 130 III International Symposium on Horticulture in Europe – SHE 2016 season with different nitrogen doses: kgN·ha- , kgN·ha- and + kgN·ha-1. Also the effect of postharvest application of elicitors (1-MCP and ethylene) was estimated. The 1-MCP treatment was conducted during hours at °C in concentration: , µl·L- , , µl·L- and , µl·L-1. Storage of fruit in atmosphere with constant ethylene concentrations (15 ppm, 50 ppm and 100 ppm) was carried out during 7 days at °C. Also the influence of different storage temperature °C, °C and °C on fruit durability was evaluated in this study. The main symptoms of tomato senescence were diseases development (rotting) and skin shriveling. Generally tomato cv. Calista F1 showed higher resistance against diseases thus better storage ability than tomato Volna F1. It was found a tendency to maintain a larger number of tomatoes with excellent quality during storage from crops fertilized with nitrogen of + kgN·ha-1 than from crops fertilized with lower N doses. The 1-MCP treatment had no effect on the delay of senescence, disease development, thus the improving of fruit storage stability. Seven days storage of tomatoes in atmosphere with ethylene addition has not contributed to acceleration of the senescence and reduction the commercial value of the fruit. Comparing the effect of storage temperature on the stability of tomato, the highest commercial value retained the fruit at °C both cv. Calista F1 and Volna F1). Tomato cv. Calista F showed similar storage stability at temperatures °C and °C, but tomato cv. Volna F significantly better preserved at °C than °C. This study was developed within the SUNNIVA project of the European framework ERA-NET SUSFOOD and supported by the NCBiR – Poland. T2-P37 THE EFFECT OF TWO BORON COMPOUNDS ON GROWTH AND CHEMICAL COMPOSITION OF TWO FRUIT ROOTSTOCKS Georgios Sismanidis 1, Sofia Ntoumanidou 2, Vasileios Christodoulou 3 ,Christos Chatzissavvidis 4 1 Pindou 3, Limassol, Cyprus 2 Georgiou Kondyli 43, Alexandroupolis, Greece 3 Venizelou 38, Stilida, Greece 4 Pantazidou 193 str, 68200 Orestiada, Greece The aim of this work was to determine the effects of boron (B) on the physiological and anatomical characteristics of MM Malus × domestica Borkh. and sour orange Citrus aurantium L. plants. The experiment was carried out under semi-field conditions and lasted 165 days. The experimental design included seven B treatments with five replications for each treatment. The plants were grown in pots filled with a substrate of sand: perlite (1:1) and fertigated initially with a Hoagland nutrient solution. Afterwards, the plants were treated with boric acid (BA) (0, 7.5, 30 and 60 mg kg-1) and sodium borate (SB) (7.5, 30, and 60 mg kg-1). Boron treatments were applied in two doses with a month interval. At the end of the experiment, in general, sour orange B use efficiency (BUE) presented a negative correlation with added B. On the other hand, BA treatments (30 and 60 mg kg-1) led to decreased B use efficiency in MM106 plants. The BA and SB treatments (60 mg kg-1) led to increased leaf and stem B concentrations of MM106 plants. Furthermore, the SB treatments (30 and 60 mg kg-1) and BA treatment (60 mg kg-1) showed the highest B concentration in all parts of sour orange plants. However, high B doses caused B toxicity symptoms in leaves. SB treatments increased leaf K concentration in both genotypes. Fresh matter weight of MM106 plants was negatively correlated with the applied B amount, while in sour orange no effect was observed. None of the rest growth parameters (plant height, stem diameter, shoot length, lamella length and the number of leaves and nods) of MM106 plants were particularly affected by B nutrition. Chlorophyll concentration in the leaves of both genotypes was increased in all B-treated plants and especially those treated with SB. Additionally, SB (7.5 mg kg-1) was the only treatment which increment significantly the photosynthetic rate of MM106 plants. Overall, the results of this study indicated that the SB-treated plants of both genotypes had better performance than BA-treated ones. 131 BOOK OF ABSTRACTS T2-P38 THE EFFECT OF ZEOLITE, CHEMICAL FERTILIZER AND COMPOST ON YIELD OF LETTUCE, NUTRIENT COMPOSITION AND SOIL PROPERTIES Victor Kavvadias, Zaxarias Ioannou, Aggeliki Mastoraki, Dimitrios Salivaras, Sideris Theocharopoulos Hellenic Agricultural Organization-DEMETER, Department of Soil Science of Athens, 1 S. Venizelou Street, 141 23 Lykovrisi, Attiki, Greece; The proper management of treated agricultural wastes (e.g. composts) contributes to the protection of the quality of water and soil, by reducing the use of chemical fertilizers, thus reducing the leachate and protecting renewable and not renewable resources. Moreover, natural zeolites and particularly clinoptilolith, due to its unique properties, can be used in agriculture in order to improve soil quality and to increase yields. In 2014, in the Department of Soil Science of Athens (central Greece) a greenhouse soil pot experiment was conducted in order to investigate the effect of natural zeolite s clinoptilolith application and fertilization (chemical N-P-K, and compost from olive leaves) on the yield of lettuce, plant nutrients and soil properties. Two rates of olive composts (0% and 10% v/v), three rates of zeolite (0% and 2% and 5 % w/w) and two chemical fertilization regimes (chemical N-P-K and no chemical fertilization) were combined. In addition, two different soils, one slightly acid (pH=6.1) and sandy loam in texture (Ac-LT) and the other slightly alkaline (pH=7.4) and sandy clay in texture (Al-HT), were introduced. Thus the behaviour of the above treatments in these two different soils was also studied. Results showed that zeolite application substantially improved yield in Ac-LT soil and in treatments without chemical fertilization; however, in other soil fertilizer combinations zeolite reduced yield. Addition of 5% w/w zeolite reduced significantly pH in Ac-LT soil, while it significantly increased pH in Al-HT. Zeolite increased significantly CEC of both soils. Fertilizer had also a positive and significant effect on CEC in Al-HT soils. In treatments without chemical fertilization zeolite did not affect EC, while a significant and positive effect was determined in treatments with compost or inorganic fertilization. Zeolite application did not influence organic matter (OM) in Ac-LT soils, but increased it significantly in Al-HT soils in particularly under chemical fertilization. With regards to soil N, in Al-HT soils zeolite application reduced it. Moreover, zeolite enhanced plant N in Ac-LT and decreased it in Al-HT. High concentrations of Na were determined in both soils and plants. Zeolite has a positive and substantial effect on exchangeable Na. Plant Na was also increased, particularly in Ac-LT soils. Zeolite increased significantly available K in Ac-LT soil but it had no effect on Al-HT soil. However, the opposite trend was observed for plant K. Soil and plant P was not affected by zeolite. It is concluded that the positive effects of zeolite on yield and on soil and plant nutrient status was related to the type of soil and fertilizers. T2-P39 THE EFFECTS OF DIFFERENT NITROGEN AND POTASSIUM LEVELS ON YIELD AND QUALITY OF TWO EARLY GRAPE CULTIVARS GROWN IN DIFFERENT SOILLESS MEDIA Serpil Tangolar, Semih Tangolar, Melike Ada, G“zin Tarim, Ayfer Alkan Torun, Ebru Ertargin, Cukurova Universty Faculty of Agriculture, Department of Soil Science and Nutrition, 01330 Adana, Turkey In this research, Trakya ilkeren (Ti) and Yalova incisi (Yi) cultivars in three growing media namely Pomza (Basaltic tuff), Cocopeat, Perlite:Peat (2:1) mixture were grown using Hoagland nutrient solution under plastic cover. Additionally, two nitrogen (N) levels (100 and 200 ppm) in Yi, and two potassium (K) levels (150 and 300 ppm) in Ti were tested. For obtaining the effect of treatments, grape yield, cluster weight, lenght and width, berry weight and volume, total soluble solids (TSS), acidity and pH were examined. Considering grape yield and cluster weight, the best results were obtained from Cocopeat and Perlite:Peat mixture for both varieties. The concentrations of 100 ppm N in Yi and 150 ppm K in Ti gave higher values than the other treatments. For Cocopeat x 150 ppm K treatment, grape yield, cluster weight, TSS%, acidity% and pH was 32.4 t.ha-1, 374.7 g, 14.37, 0.584 and 3.47, respectively. In the same order, values of Perlite:Peat 132 III International Symposium on Horticulture in Europe – SHE 2016 mixture x 150 ppm K took place as 30.5 t. ha-1, 352.6 g, 13.70, 0.582 and 3.55. In terms of N treatments of Yi cultivar, values in the same order mentioned above were 40.7 t. ha-1, 407.8 g, 12.63, 0.405 and 3.67 for Cocopeat x 100 ppm N; 44.5 t.ha-1, 445.4 g, 15.17, 0.413 and 3,82 for Perlite:Peat x 100 ppm N treatment. Berry weight and volume were not clearly affected by the N and K levels. Results showed that the soilless culture can provide high yield for table grapes without negative effects on cluster and berry quality. T2-P40 THE EFFECTS OF POSTHARVEST WATER SUBMERGING AND 1METHYLCYCLOPROPENE TREATMENTS ON THE RIPENING OF `GOLDEN DELICIOUS´ APPLES FROM TWO HARVEST DATES Daniel Alexandre Neuwald1, Leonie Hart2 1University of Hohenheim, Physiology of specialty crops at the Competence Centre for Fruit Growing - Lake Constance, Ravensburg, Germany; 2University of Hohenheim, Emil-Wolff-Str. , Bad W“rttemberg Stuttgart, Germany. Golden Delicious apples sometimes develop skin burning symptoms during postharvest handling and storage. Apples were picked on 30 September 2015 following a period of dry sunny weather and on 07 October directly after 18 mm of rainfall. At-harvest, fruit were submerged under H2O for 48h or not submerged then treated or not treated with 1-MCP (650ppb for 24h). Treated fruit plus untreated controls (UTC) were then maintained for 14d shelf-life SL at °C or placed in controlled atmosphere CA storage °C; kPa O ; . kPa CO for seven months. After d the SL treatments were assessed for fruit firmness (FF) and physiological disorders. The 1-MCP and the H2O + 1-MCP treatments maintained higher FF when compared to UTC from both harvest dates. The SL submerged treatments (-1-MCP & +1-MCP) harvested at the later date after rain showed lower FF and a higher disorders incidence compared to the first harvest. While the SL -1-MCP and +1-MCP treatments from both harvest dates showed a low incidence of skin burning, the also for both harvest dates SL H2O + 1-MCP treatment showed a high incidence of scald, skin burning, internal browning, core-flush but only a small amount of CO2 skin burning. Submerging fruit after harvest and before storage increases the disorder incidence but does not markedly change FF, as the efficacy of the 1-MCP was still maintained. Our preliminary results after 14d SL show that 1-MCP can maintain FF in early or late harvested fruit even when fruit were submerged in water. Submerging apples under water increases the disorder incidence while 1-MCP has no affect. CA storage results be assessed May 2016. T2-P41 THE EFFECTS OF PREHARVEST 1-METHYLCYCLOPROPENE (HARVISTA) TREATMENTS ON HARVEST MATURITY AND SHELF LIFE QUALITY OF `SANTA MARIA´ PEAR CULTIVAR Mustafa Sakaldas1, Engin G“r2, Mehmet Ali G“ndodu3 1Canakkale Onsekiz Mart University , Faculty of Agriculture, Department of Horticulture, 90 17100 Canakkale, Turkey; 2anakkale Onsekiz Mart University, Lapseki Vocational School, 17800 anakkaleLapseki, Turkey; 3anakkale Onsekiz Mart University, Faculty of Agriculture , Department of Horticulture, 17020 anakkale, Turkey. In this research, the effects of preharvest 1-Methylcyclopropene (1-MCP) (Harvista) treatments on fruit drop, fruit quality and shelf life of Santa Maria pear cultivar were studied. For this purpose, (arvista treatments at doses of 50, 100, 150 and 200 g ha- were applied to Santa Maria pear cultivar in G“rsu, Bursa region, Turkey. Besides Naphthalene acetic acid (NAA) with 53.5 ppm dose was applied as comparison. Samplings were carried out on trees 1 day before and 7, 14, 28 and 42 days after applications respectively. Fruit drop rate, 133 BOOK OF ABSTRACTS flesh firmness, soluble solids content, titratable acidity, skin colour, total phenolic compounds and ethylene production were evaluated after each sampling date. Furthermore, fruits were kept at 20- °C temperature and 50-60% relative humidity conditions as shelf life for 7 and 14 days respectively. According to the results, Harvista applications with 150 and 200 g ha-1 doses were found out as the most positive applications because of preventing fruit drop and minimizing the changes of quality parameters. Harvest maturity could be prolonged for 14 to 28 days with these application doses. T2-P42 THE EFFECTS OF TOPPING APPLICATIONS AT DIFFERENT LEVELS ON YIELD AND QUALITY OF SUMMER SHOOTS FOR YALOVA CEKIRDEKSIZI GRAPE CULTIVAR Arda Akçal Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 ANAKKALE, Turkey This research was conducted on the vines of Αalova Çekirdeksiz grape cultivar, which was grafted on 5BB root stock, in the Table Grape Cultivars Resarch Vineyards of COMU Agriculture Faculty in Çanakkale, in betwwen the years of 2014 and 2015. This vineyard was trained by the constant one-armed cordon training system and planted at 3.0 meters x 1.5 meters apart. After berry formation, 3 different topping application was treated on summer shoots of Αalova Çekirdeksizi grape cultivar. These were; SLT :Short Level Topping topping over the first level of the lay wire in summer shoots), NLT : Normal Level Topping (topping over the second level of the lay wire in summer shoots), HLLS: High Level Leaving Shoots (tied on summer shoots without topping over the second level of the lay wire) treatments. As a result, there was not any significant effect on average grape yield, but some significant differences were determined on the parameters of average cluster weight, cluster density, width and height of the cluster, width of the berry, color of the berry (L, C and H values), % SSDM Soluble solid dry matters, %acidity, pH, maturity index (% SSDM / %acidity), weight of the prunning woods and diameter of the annual branches. In HLLS application, maturity index value of the grapes, harvested at the same dates were increased at a significant level, considerably in comparison to the other applications. In SLT application, average cluster weight, cluster density, width of the cluster and berry parameters were also high considerably in comparison to the other applications, but weight of the prunning woods and diameter of the annual branches parameters were significantly lower. T2-P43 THE INFLUENCE OF CROWN SHAPES ON YIELD QUANTITY AND QUALITY PARAMETERS Cristina Moale Research Station Fruit Growing Constanta, No. 25 Pepinierei Street, Valu lui Traian, 907300 Constanta, Romania The quality of the fruit is an ensemble of specific traits and characteristics which, together with other criteria, influences the choice and the promotion of the assortment, depending on the demand on the market. The growth of nectarine consumption depends on the commercial value, quality and the production costs of these fruit. Four nectarine tree cultivars were studied, three of which are Romanian cultivars, created and homologated at R.S.F.G. Constanţa Cora , Delta and Romamer , the other cultivar of American origin Crimsongold . Phenological observations were performed concerning: a vegetative phenophases swelling of the vegetative buds, beginning of the shoots growth, end of the shoots growth; b) fructification phenophases – beginning of swelling, beggining of flowering, end of flowering, intensity of flowering, hardening of stone, beginning of ripening, harvesting maturity. The quality of the fruit was also determined: the average weight of the fruit, the average weight of the stones, stones percentage, dry matter, titrable acidity. The purpose of this study was to evaluate the qualitative potential of some nectarine tree cultivars with early ripening Cora , Delta , Romamer and Crimsongold by means of determining the quality of the fruit. The dry matter had values ranging between % for the fruit of the Crimsongold cultivar and . % for Romamer , the variation between cultivars being reduced. Within the canopy shape of the trees 134 III International Symposium on Horticulture in Europe – SHE 2016 which were studied, the average acidity values was 0.89% as average for the years 2008-2011 for the shape Improved Vase and 0.91 g% for Tatura and Veronese Vase. It was noted that the shape Tatura might influence the size of the fruit, this shape having larger fruit (98.2 %g/g), whereas the Vertical Vase recorded the smallest weight of the fruit, 92.2 %g/g. T2-P44 THE ROLE OF MULCHING MATERIALS ON LACTUCA SATIVA PRODUCTION BRAZIL CASE STUDY Paulo Fortes Neto, Nara L’cia Perondi Fortes, Kaio Pinheiro, Arthur Saraiva, Raquel Costa, Elizabeth Duarte Av. )t|lia TAUBATÉ V)LLAGE R RUA , N , SO PAULO, BRAΒ)L, Taubaté-12, Brazil; In Brazil, the commonly used mulches in the lettuce production are vegetable scraps or polyethylene film. In opposition, the use of biodegradable mulch films is increasing in Europe due to environmental problems associated to the conventional ones. Few studies regarding the application of these new mulches on horticulture crops in Brazil has been reported, which is a great barrier for the acceptance by producers. The main objective of this study was to fulfil this lack of knowledge, by the evaluation of the performance of different mulches on lettuce production. For this purpose, were followed, throughout lettuce production cycle, the soil temperature, water volume and nitrogen content, mulch durability and deterioration, weed suppression, and crop yield. A full-scale field experiment was set up in the Taubate University Campus, to evaluate the suitability of three modalities for mulching application in lettuce production in comparison with bare soil. The experimental design used were randomized blocks with five replicates of all the treatments: (T1) bare soil, (T2) cover with rice husk, (T3) cover with polyethylene film and (T4) cover with biodegradable film. The variety used was crispa, with a cycle of 50 days and starting after the transplantation occurred on 22th September 2014. From results obtained, we observed that soil temperature and water volume content were higher on film modalities (T3 and T4). Regarding nitrogen soil content, we observed that the lowest value was achieved on T2 modality. As expected, the weed control was higher in film modalities and the mulch films mechanical properties were kept along the crop cycle. Regarding average production of each modality, the highest values were obtained with T4 with 945 g/plant followed by T2 with 823 g/plant, T3 with 733 g/plant, and T1 with 575 g/plant, respectively. Overall, we concluded that biodegradable mulch film, compared to Polyethylene and rice husk, was more effective. T2-P45 VEGETO-PRODUCTIVE BEHAVIOR AND FRUIT QUALITY OF TWO CULTIVARS OF APRICOT, `ORANGE RUBIS®´ AND 'FARBALY®', AS INFLUENCED BY DIFFERENT BIOSTIMULANTS Giuseppe Lopriore, Francesco Lops, Annalisa Tarantino Via Napoli, 25, 71122 Foggia, Italy Biostimulants, products with specific action, natural or synthetic, mineral or organic, have been introduced recently as fertilizers that improve the growth of plants using different mechanisms when applied in small quantities. This trial aims to evaluate the effect of four biostimulant products Bion® WG, (endophit® PS, Ergostim® and Radicon® on two cultivars of apricot Orange rubis® and Farbaly® , grown in experimental field at Cerignola (Foggia district, Apulian region, Southern Italy). The biostimulants used were related products of humic acids that induce effects on yield, fruit quality and grower income of some fruit trees. Several foliar treatments were applied at different time during the growth season of each crops. Total yield of fruits per tree was also determined. Other than that related with vegeto-productive behavior, many fruit quality parameters were evaluated, including also total polyphenols content and antioxidant activity. Experimental data showed some specific differences among the biostimulant treatments. Regarding the productive behavior, the Farbaly® cultivar when treated with Ergostim® showed higher yield respect to 135 BOOK OF ABSTRACTS the control treatment. The soluble solids content in Farbaly® was positively affected by Bion® and (endophyt® treatments while no effect of biostimulants treatments was observed in Orange Rubis . )n accordance with these values of soluble solids content, fruit firmness at the first harvest date was tendentially lower than the control in Orange Rubis® treated with Bion® and (endophyt® and always lower in Farbaly® for all the biostimulant treatments. At the second date harvest almost all the biostimulants treatments in both Orange rubis® and Farbaly® cultivars showed values lower than the control. A positive effect on polyphenols content was observed in consequence of all biostimulants treatments and particularly on fruits of Orange rubis® treated with (endophit® whit increments of more than % of total polyphenols content T2-P46 YIELD OF ONION (ALLIUM CEPA L.) GROWN FROM DIRECT SEEDING AND SOME SOIL PROPERTIES AS AFFECTED BY LONG-TERM ORGANIC AND/OR MINERAL FERTILIZATION Kazimierz Felczynski, Waldemar Kowalczyk Institute of Horticulture, Konstytucji 3 Maja 1-3, 96-100 Skierniewice, Poland. Poland is counting among the biggest onion producers in Europe. Experiments with this vegetable were conducted in 2007, 2011 and 2015 on the plots of long-term static fertilization experiment, which was established in Skierniewice in 1922 and has been continued uninterruptedly up to the present in various cycles and with different vegetable species. Consecutive 12 – year cycle of investigations began in 2005 with 4 vegetable species: white cabbage, snap been, onion and sweet corn in crop rotation. Response of onion plants to long-term organic, mineral and organic-mineral fertilization applied at different rates was determined. There were evaluated also some soil properties like organic matter content, volume weight, and field water holding capacity. In each year of experiment the highest marketable yields of onion were obtained from the treatments with combined fertilization manure + N mineral, at rates of t·ha- + kg N·ha-1 and except the year , at rates t·ha- + kg N·ha-1. In 2007 second high marketable yield was obtained from treatment fertilized each year (already over 90 years) with cattle manure only, at rate of t·ha-1. The lowest yields were obtained from not fertilized control and then from treatment with mineral P and K fertilization only. Treatments fertilized with manure only or with manure + N mineral distinguished higher organic matter content and field water holding capacity and lower volume weight than at treatments fertilized with mineral fertilizers only. T2-P47 YIELDS AND NITROGEN UPTAKE OF CAULIFLOWER AS AFFECTED BY N RATES AND INTERCROPPING WITH PEA AND CLOVER Marco Tempesta 1, Giorgio Gianquinto 2, Markus Hauser 3, Massimo Tagliavini 1 Faculty of Sciences and Technology , Free University of Bolzano, Piazza Universit{, , , Bolzano, )taly 2 Department of Agricultural Sciences DipSA, University of Bologna, Viale Fanin, 44, 40127 Bologna, Italy 3 Laimburg Research Centre of Vegetable Crops, Via Stazione, 39023 Oris BZ, Italy Cauliflower Brassica oleracea L. var. botrytis is one of the world s most spread brassica species and one of the main vegetable crop cultivated in the mountain environment. Cauliflower generally requires large amounts of nitrogen (N) fertilizer to obtain high crop yield both qualitatively and quantitatively. Intercropping cauliflower with a leguminous species may help farmers to reduce N leaching, production costs and to prevent environmental pollution. In this study, cauliflower (cv Seoul F1) was cultivated alone or intercropped with either pea (Pisum sativum cv Progress 9) or clover (Trifolium resupinatum cv Gorby), using five N fertilization rates. The experiment was carried out in a mountain environment (Val Venosta, South Tyrol, Italy) with a 5 (N rates) x 3 (cropping systems) split-plot factorial experiment with three replications. Nitrogen rates were 0, 75, 150, 225, 300 kg N ha-1 (later referred as N0, N1, N2, N3, N4, respectively). Nitrogen rates, but not the cropping system, had an effect on curd yields (from 29 to 45 t ha-1), 136 III International Symposium on Horticulture in Europe – SHE 2016 which strongly increased from N0 to N2, but decreased again if N rates were higher than 150 kg N ha-1 (N3 and N4). N uptake rate, assessed by N content of plants at harvest, was affected by both the N rate, the cropping systems and their interaction. Under no N supply (N0) cauliflowers depressed their N uptake due to the intercropping with both legume species, but N uptake by cauliflower was unaffected by intercropping if N rates were N1 and N2. Increasing N rates progressively increased N uptake if cauliflower was grown alone, but not when it was intercropped. Results suggest that intercropping with legume plants is a promising technique to reduce the need for external N input in cauliflower production, with potential beneficial effects on soil fertility for the crops in succession. T2-P48 REDUCING POSTHARVEST LOSSES BY FILM PACKAGING OF AFRICAN NIGHTSHADE (SOLANUM SCABRUM MILL.) ‘IMPROVEMENT OF STORABILITY AND POSTHARVEST QUALITY Christian Ulrichs 1, Susanne Huyskens-Keil 1, Bernhard Trierweiler 2, Arnold Opiyo 3, Elisha Gogo1 1 Humboldt-University Berlin, Division Urban Plant Ecophysiology, Lentzeallee 55-57, 14195 Berlin, Germany 2 Max Rubner-Institute, Institute of Nutrition and Food, Haid-und-Neu-Str. 9, 76131 Karlsruhe, Germany 3 Egerton University, Department of Crops, P.O. Box 536, 20115 Egerton, Kenya Indigenous leafy vegetables, e.g. African nightshade (Solanum scabrum Mill.) gain importance due their high nutritional, health, and economic value in Kenya, contributing to secure food supply and livelihood of smallholder farmers in rural and urban/per-urban areas. Inadequate postharvest handling, facilities for storage and transport, insufficient hygiene conditions, and poor infrastructure aggravate these problems causing high postharvest losses (50%) of these highly perishable crops. Studies were conducted to evaluate the effect of film packaging Xtend® on improving storability and postharvest quality and reduce postharvest losses of African nightshade cv. Olevolosi. Plants were cultivated in a climate chamber (4 weeks), and thereafter under greenhouse conditions (3 weeks) where harvest was conducted seven weeks from sowing. Leaves were stored either packaged in modified atmosphere packaging bags Xtend® or left open control for days ˚C; R( % and days ˚C; R( % . Weight loss, color, antioxidative compounds -carotene, lutein, lycopene, chlorophylls), and protein content were determined at harvest, and 2, 4, 8 and 14 days after storage. Concentrations of CO2 and O2 within packaging were determined continuously. Results revealed packaging of African nightshade leaves retained leaf color at °C and reduced weight loss at °C . % and °C . % on days and , respectively, compared with the control % . Moreover, film packaging maintained or increased antioxidant contents, i.e. -carotene, lutein, lycopene, chlorophylls at °C for days of storage, whereas at °C carotenoids and protein increased within 2 days, but thereafter declined. CO2 and O2 concentrations were 4.7% and 17%, respectively, an indication of reduced respiration rates of leaves in film packaging compared to unpacked leaves. Findings provide a high potential of using Xtend® packaging to improve storability and nutritional quality of African nightshade which is a vital step to improve nutritional, health and economic status of developing countries such as Kenya. 137 BOOK OF ABSTRACTS Poster Topic – 3 Genetic Resources, Systems Approaches & Biodiversity 138 III International Symposium on Horticulture in Europe – SHE 2016 T3-P1 IDENTIFICATION OF GEOGRAPHICAL ORIGIN OF DRY BEANS USING THE ISOTOPE RATIO ANALYSIS Athanasios Lantavos, Katerina Kiriakou, Kostas Salmas, Angelos Patakas University of Patras, Lab of Plant Production, G. Seferi 3, 30100 Agrinio, Greece. Determination of the geographical origin of agricultural products has been a growing issue over the past decade mostly due to the increased concern of consumers about food authenticity. Isotope-Ratio Mass spectrometry (IRMS), utilized to determine the isotopic relationship of stable isotopes, is considered as an extremely useful analytical technique for differentiation between the different geographic origins of various agricultural products. The isotopes and their ratio most commonly used in origin assessments are those of the light elements. In our study a comparative analysis of stable isotope ratios 13C/12C, 15N/14N and 34S/32S was performed in dry beans (Phaseolus vulgaris L.) originated from different regions in Greece (Prespes and Kastoria), China and Kirgistan using an IRMS coupling elemental analyzer. All isotope ratios were expressed as / against international standards. Results indicated significant differences in N and S while no significant differences in C between samples were obtained. )n partricular, China originated beans exhibited lower values of N followed by those from Kirgistan. As far as S is concerned, Prespa s beans exhibited the lowest values Results suggested that combining the isotopic ratios of S and N a good discrimination between dry beans from the regions sudied could be achieved. T3-P2 A COMPARATIVE STUDY AMONG DIFFERENT OLIVE CULTIVARS (OLEA EUROPAEA L.) REVEALS DIFFERENCES IN EXPRESSION LEVELS OF GENES INVOLVED IN FRUIT VITAMIN E BIOSYNTHESIS Egli Georgiadou1, Chrysa Sergendani2, George Koubouris2, George Manganaris 3, Panagiotis Kalaitzis4, Vlasios Goulas5, Vassilis Fotopoulos1 1Anexartisias 57, Lemesos, Cyprus; Chania, Greece; 3Anexartisias 33, P.O. Box 50329, 3603 Lemesos, Cyprus; 4Mediterannean Agronomic Institute of Chania, Chania, Greece; 5Cyprus University of Technology, Lemesos, Cyprus. 2NAGREF, The current study compares expression of genes involved in vitamin E biosynthesis, oil and tocochromanol content, as well as the antioxidant capacity in the fruit flesh of five olive cultivars Koroneiki , Kalamata , Mavrelia , Kalokairida and Lianolia Kerkyras based on skin color green-yellow, yellow-purple, purple, black and weight of black fruit. Representative healthy fruit samples ‐ kg were harvested in triplicate around the canopy at human height between the beginning of November and the middle of December. Morphological measurements were conducted according to UPOV (1999) TG/099/3. The oil content was determined by Soxhlet extraction and was expressed as a percentage of dry and fresh olive paste weight, while a fruit flesh subsample was stored at ‐ oC for further analyses. Koroneiki and Mavrelia fruits had the lowest weight and diameter but they had the highest oil content as a percentage of dry and fresh olive paste weight compared with the other cultivars. Antioxidant capacity of the olive cultivars was measured with the methods of DPP( and TEAC. )ncreased levels of antioxidant capacity were observed in cv. Lianolia Kerkyras fruits, while the lowest values were recorded in cv. 'Mavrelia' with both assays. For the molecular analysis of tocochromanol biosynthesis in fruit flesh, real‐time RT‐PCR qRT‐PCR was performed using UBQ2 as a reference gene. Gene expression studies showed a general down‐regulation of tocochromanol biosynthetic genes in all cultivars examined across all skin colors compared with green-yellow skin color, with the exception of (PPD in cv. Lianolia Kerkyras and the genes (PPD and VTE on skin color yellow‐ purple in cv. Kalamata and the VTE in cv. Koroneiki . Chromatographic quantification of tocochromanol content by HPLC is expected to further elucidate the vitamin E metabolite profiles from each olive cultivar. 139 BOOK OF ABSTRACTS T3-P3 A PISTACHIO SELECTION FROM THE UNIVERSITY OF CALIFORNIA BREEDING PROGRAM SHOWS PROMISE AS A COMMERCIAL CULTIVAR FOR EARLIER HARVEST MATURITY Craig Kallsen 1, Dan Parfitt 2, 1 University of California, 1031 S. Mt. Vernon Ave., Bakersfield, CA 93307, United States of America 2 Dept. of Plant Sciences, MS2, Univ. of California, One Shields Ave., Davis CA 95616, United States of America The University of California pistachio breeding program is a comprehensive scion cultivar development program. One objective of the program is development of new cultivars that produce a crop maturing earlier than that of existing cultivars. Prior to 2004, more than 98% of the pistachio industry in the United States was composed of the single female cultivar Kerman . )n , the earlier-harvesting U.C. cultivars Golden (ills and Lost (ills were introduced. These cultivars now comprise about % of all California orchard and a much higher percentage of new plantings. Golden (ills and Lost (ills mature up to two weeks earlier than Kerman with yield equivalent or better than Kerman . The advantage of earlier nut maturity is that it increases the efficiency of the industry-wide harvest by extending the harvest season and thus reducing peak demand for labor, equipment and nut processing facilities. Damage by navel orangeworm (NOW), Amyelois transitella, increases exponentially with each reproductive cycle in infested orchards and earlier harvest has prevented exposure to the last NOW cycle during some years. Earlier bloom and harvest may also be correlated with a lower winter-chill requirement which will be important in a warming California climate. In addition to an earlier harvest, new cultivars must meet existing standards for yield and not quality characteristics, such as high inshell split nut percentage, low blank-nut production, good shell-hinge strength, clean shells and more. A new early-maturing selection, along with several pollenizers, was planted in 2007 in a replicated and randomized trial in the southern San Joaquin Valley of California. In four years of experimental harvests, this early-maturing selection demonstrated that it is possible to develop a cultivar with commercial potential that is ready for harvest days before Golden (ills and Lost (ills , and three weeks or more before Kerman . This cultivar is being released to California growers this year. T3-P4 ADAPTATION OF OREGANO (ORIGANUM VULGARE L.) TO CULTIVATION IN ELVAS REGION, SOUTH PORTUGAL Orlanda Viamonte Póvoa 1, Noémia Farinha , C|tia Claré 1 Esola Superior Agraria de Elvas, Quartel do Trem., AP 254, 7350 Elvas, Portugal 2 Edifício do Trem Auto, Avenida de Janeiro, -903 Elvas, Portugal, 7350-903 Elvas Portalegre Elvas, Portugal Origanum vulgare L. species is used as an herb, spice and medicinal plant, occurring spontaneously in the Alentejo region. Its sustainable cultivation should be promoted in order to reduce genetic erosion caused by harvest in nature. The main goal of this study was to respond to the request of a farmer from Elvas region, focusing on the acessment of the adaptation to cultivation of six acessions, three spontaneous (OV1 from Vila Fernando, OV2 from Estremoz, OV3 from Vila Boim) and the remaining three commercial (OVT1, OVT2, OVT3). Plant propagation was carried out from stem cuttings for the three spontaneous acessions and from seed for the three commercial acessions. Using material from OV2, a stem cutting vegetative propagation trial was carried out, using softwood (terminal and sub-terminal) and semi-hardwood (basal) stem cuttings; cuttings survival and growth was observed. Seed germination was tested using the 3 commercial seed accessions and the spontaneous accessions harvest in nature and after cultivation at °C with h photoperiod. The following descriptors were observed on the filed characterization essay: flowering date; length, width and color of the basal leaf; growth habit; width and height of the plant; length of the main stem, length and width of the terminal inflorescence, flowered portion of the stem length and width; plant biomass (fresh and dry). The subterminal stem cuttings survived better (83.8%) and survival rate was significantly higher than basal cuttings (56.8%). Seed germination varied from 69.5% to 92%, with significant differences between the 140 III International Symposium on Horticulture in Europe – SHE 2016 commercial seeds and seeds of the spontaneous accessions after cultivation. On the characterization field essay, the flowering date was the only descriptor with significant differences between spontaneous acessions. The earliest- flowering acession was OV1 and the later-flowering acession was OV2. The difference between the flowering dates of these acessions was 13 days. The work provided useful information to the farmer for oregano growing, however the study should be continued. T3-P5 AN INTEGRATIVE APPROACH TO UNDERSTAND POSTHARVEST RIPENING HETEROGENEITY OF HASS AVOCADOS Romina Pedreschi1, Claudia Fuentealba1, Ignacia Hernandez1, Bruno G Defilippi2, Reinaldo Campos-Vargas3, Susan Lurie4, Sebastien Carpentier5 Pontificia Universidad Católica Valparaíso, Calle San Francisco sn La Palma, Quillota, Chile 2 Instituto de Investigaciones Agropecuarias , INIA La Platina , Santiago, Chile Centro de Biotecnología Vegetal, Universidad Andrés Bello, Av. Rep’blica Santiago, Chile 4 Department of Postharvest Science , Volcani Center, Bet-Dagan, Israel 5 Department of Biosystems, Willem de Croylaan 42, Leuven, Belgium The complex physiology of avocado renders a heterogeneous product at harvest. This heterogeneity is only evidenced during postharvest management as variable ripening which results in logistical problems for marketers and inconsistent quality delivery to consumers. This problem is being addressed with holistic approaches but more insights into the mechanisms involved are necessary. Thus, the main research objectives were: (i) to link targeted metabolites and enzymes with ripening rate (slow vs fast ripening fruit) and (ii) to study at the proteome level the differences observed between these two contrasting phenotypes. Biopsies of fruit flesh were taken immediately after harvest and after d storage at °C in CA % O and % CO . Ripening was followed at °C, % R(, and the days to reach edible ripeness on each individual fruit were recorded. Early (23-26% DM), middle (27-30% DM) and late (>30% DM) season fruit were evaluated. The very fast and very slow ripening fruit ≈ replicates were compared in terms of pectin methylesterase (PME) and polygalacturonase (PG) activities, total soluble protein content, fatty acids and gel-free fruit proteome. Results indicated that CA stored fruit had less variability compared to recently harvested fruit. No statistical differences in terms of total soluble protein or PME activity were found between very fast and very slow ripening fruit but PG activity but concentrations of certain polyunsaturated fatty acids differed. Evaluation of the whole proteome is in progress. The approach is to find initial quality markers at the metabolome or proteome level to predict ripening heterogeneity. In addition, non-destructive techniques, which can measure on-line these markers, would allow sorting the fruit to reduce ripening heterogeneity in avocado batches. T3-P6 ARTICHOKE TRANSPLANT PRODUCTION: EFFECTS OF NUTRIENT SOLUTION DELIVERY AND LEAF CLIPPING Giuseppe Colla1, Mariateresa Cardarelli2, Walter Stefanoni1,Antonio Fiorillo1, Giudo Bernabei1, Youssef Rouphael3 1Tuscia University, Viterbo, Italy, Viterbo, Italy; 2Crea Roma, Rome, Italy; 3Via Universit{ 100, 80055 Portici,IT, 80055 Portici(Napoli), Italy Advanced growing systems such as floating system widely used to produce leafy vegetables and tobacco transplants may be a good solution for producing globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] transplants due to the labor saving and rapid plant growth. Leaf clipping is another technique used in 141 BOOK OF ABSTRACTS tobacco seedling production to reduce height and increase seedling uniformity and quality. Clipping involves removal of some of the plant canopy, while leaving the apical meristem. Two experiments were carried out at Tuscia University. In the first, the effects of two nutrient solution delivery methods (overhead sprinkler system or subirrigation through a floating system) and leaf clipping (with or without) of two artichoke seedpropagated F hybrids )star and Romolo were compared in terms of plant growth, SPAD index and chlorophyll fluorescence. The second experiment was aimed to assess the tolerance of artichoke transplants coming from the four treatments to water stress. The effect of leaf clipping was less pronounced than the nutrient solution delivery. When averaged over leaf clipping, the shoot dry weight, the root-to-shoot ratio and the maximum quantum use efﬁciency of PS)) were significantly higher by 77%, 75% and 10% for Romolo and by %, % and % for )star with floating raft system compared to the overhead sprinkler system. An opposite trend was recorded for the chlorophyll content, since the lowest values of SPAD index were recorded in artichoke transplants grown in the floating system. Artichoke transplants responded in a similar way to water stress after planting indicating that the nursery production system had a little effect on water stress tolerance after planting. T3-P7 BIOCHEMICAL CHARACTERIZATION OF LEAVES, BURNS AND SHELLS OF BOUCHE DE BETIZAC CHESTNUT CULTIVAR Bruna Laratta, Filomena Monica Vella, Luigi De Masi, Alessandra Morana, La Cara Francesco CNR Istituto di Biologia Agroambientale e, Forestale, via P. Castellino, 111, 80131 Napoli, Italy. Bouche de Betizac is the French-Japanese hybrid Castanea sativa x Castanea crenataand, even ifit is pollen sterile, it is a consistent producer of large crops of big nuts that are easy to peel and are preferred for their appetizing flavour and taste by consumers. This cultivar is well adapted to growth in Europe, North America and Asia and it has demonstrated to survive to cold season, producing chestnuts with excellent yield and nut quality. The most beneficial characteristics of Bouche de Betizac are over all its resistance to the Asian gall wasp insect andto leafanthracnose caused by Phytophthora spp. Many extracts from chestnut tree components, including fruit, burns, leaves, etc. might be used for human health diseases prevention. It has been demonstrated that chestnut leaves contain phenolic compounds that possess antioxidant properties with antiradical activity and, several of them, antibacterial action. The aim of this work was to perform a biochemical characterization of leaves, burns and shells (outer and inner of the Bouche de Betizac chestnuts. Samples of this variety from an orchard located in Roccamonfina Regional Park in Southern )taly, were utilized for the analysis. The extracts from all chestnut components were evaluated for the antioxidant power, total phenolic, ortho-diphenolic, flavonoid, and tannin contents to assess the presence of bioactive molecules. The results obtained showed that inner shells possess twice as antioxidant activity of the other chestnut components and the higher phenolic compounds content followed by leaves and burns. A comparison with the literature data is difficult due to the differences in cultivar, stadium of fruits harvesting and local climate conditions. However, at our knowledge, this is the first study reporting the biochemical characterization of the Bouche de Betizac hybrid. Nevertheless it represents a dangerous risk for biodiversity of Italian chestnut cultivar, considering its resistance to several pests and diseases, and the great capacity of adaption to growth in a wide range of climate condition, the results obtained are very promising. 142 III International Symposium on Horticulture in Europe – SHE 2016 T3-P8 BIOLOGICAL AND ECONOMIC CHARACTERISTICS OF NEW PEACH CULTIVARS AND HYBRIDS IN THE STEPPE CRIMEA Tatyana Latsko1, Anatoliy Smykov2, Olga Fedorova2 1297513 Crimea, Simferopol, v.Noviy Sad, Molodezhnaja str., 14-5, Russian Federation; 2Crimea, Nikita, Yalta, Russian Federation. Peach Prunus persica (L.) Batsch is introduced to the culture of the Crimean region. Initially (in the XIX century), its spread areal occupied only the southern part of it. An unsustainable winter weather and recurrent spring frost limits the spread of this culture in the steppe zone of Crimea. Foreign cultivars are often damaged by extreme, freezing temperatures in the winter and in the spring. There are not enough of domestic breeding cultivars that meet the requirements of the modern commercial horticulture. The hybrid fund was created by means of methods the classical hybridization using the European, American and Central Asian cultivars as parental: Valiant , Veteran , Kudesnik , Laureate , Miryanin , Red (aven , Start , Sanrise , Favorite Morettiny et al. The aim of our study was to distinguish new promising genotypes for the steppe zone of Crimea and to estimate their biological features and economic importance. The study of this hybrid peach fund carried out in the Steppe branch NBG. The selections were propagated through grafting onto seminal almond rootstocks. As a result the 40 of promising peach hybrids, adapted to the soil and climatic conditions of the steppe Crimean zone are highlighted. They are characterized by high productivity, good quality of fruits and excellent taste. Some of these hybrids are superior to existing domestic cultivars that are the national standards by the crop yield, the winter hardiness, the disease resistance, the appearance and the fruit quality. By results of these researches advanced selections are submitted to the State cultivar testing, and then there were registered as cultivars, such as: Vavilovskij , Demerdzhinskij , Kandidatskij , Kloun , et al. Some biological and economic characteristics (fruit size, skin, color and overcolor, flesh firmness, sugar content and titratable acidity of the fruit, yield) of these cultivars are presented. T3-P9 CHILLING REQUIREMENT OF NINE PEACH CULTIVARS GROWN IN SOUTHERN BRAZIL REGION Flavio Herter1, Chaiane Milech2, Maria do Carmo Raseira3 1EMBRAPA/CPACT, C. Postal 403, 96001-970 Pelotas, Brazil; Federal de Pelotas, Programa d, 96160-000 Pelotas-RS, Brazil; 3Embrapa Clima Temperado, BR-392, Km 78, 96010-971 Pelotas-RS, Brazil. 2Universidade Chilling requirement is the main factor which limits peach cultivation under low latitudes. For this reason, estimation of chilling requirements, in order to choose cultivars adapted to these areas, is very important. This paper presents the estimation of chilling requirements of eight Brazilian and one Florida peach cultivars, calculated by the Taiwan model (OU&CHEN, 2000). (ourly temperatures of the four coldest months of Pelotas, RS, Brazil º ´ S; ° ´ W; m a.s. . were recorded for the period 2004 to 2014. The following cultivars were used in the study: Bonão, Precocinho, Pepita, Diamante, Turmalina (all non melting yellow flesh peaches) and Marfim, Maravilha, BR-3, Coral (producers of white flesh peaches). Except for cv. Maravilha, all the others were developed by Brazilian peach breeding programs. Comparing the results obtained on different years for each cultivar with the obtained productivity, it was possible to estimate the minimum chilling accumulation in order to obtain a profitable commercial productivity. According to the results, the cv. Bonão had one of the lowest chilling requirement corresponding to 500 chill units calculated by Taiwan model; cv. Precocinho needed 471 units; Pepita needed 502 c.u.; cv. Maravilha needed 619 c.u.; cultivar BR 3 needed 707c.u.; `Coral `had a chilling requirement of c.u.; `Diamente ´needed c.u.; ´Turmalina´ requirement was c.u.; and cv. Marfim had a chilling requirement of c.u. Using as reference temperatures below . °C or °F, data not shown , which have, commonly, been adopted in Southern Brazil, the numbers are much lower ranging for a 143 BOOK OF ABSTRACTS minimum of hours for cvs. Bonão and Maravilha to less than hours for cv. Coral. This emphasizes that for low chill cultivars higher temperatures are effective in dormancy breaking (Chavarria, 2000; Ou & Chen 2000; Erez & Couvillon, 1987). T3-P10 CO-EXPRESSION NETWORKS DURING TOMATO FRUIT RIPENING Panagiotis Kalaitzis, Stilianos Arhondakis, Andreas Perrakis, Craita Elena Bita, Dimitrios Kaloudas Mediterranean Agronomic Inst. Of Chania, 85, Macedonia Str. P.O. Box 85, 73100 Chania, Greece; Module-based approaches provide fundamental information to infer key transcription factors regulating modules of genes during biological processes in plants. In this work, using in house and publicly available Affymetrix expression data, we investigated the fruit ripening of tomato (Solanum lycopersicum) in terms of significant transcriptomic changes and regulatory networks. Our analysis revealed a down-regulation of the vast majority of the differentially expressed genes from the breaker stage until that of red ripe, in which expression increased again. In addition, using LeMoNe algorithm, from an expression data matrix and a list of candidate regulators we produced a module network (co-expressed genes) and inferred two potential regulators, the WRKY22 and ER24, found to regulate distinct modules. Our results were found to be supported by qRT-PCR analyses, thus increased biological evidence. Our findings suggest that co-expression analyses represent a promising approach for assessing key regulatory networks, and may assist and prioritize the selection of candidate genes for further investigation. T3-P11 CONSTRUCTION OF AN ALMOND GENETIC LINKAGE MAP USING F1 POPULATION GULCAN-2 X LAURANNE BY SSR MARKERS Salih Kafkas1, Aibibula Paizila1, Elmira Ziya-Motalebipour2, Izzet Acar3, Nurgul Turemis2 1Department of Horticulture, Faculty of Agriculture, University of Cukurova, TR-01330 Adana Balcali, Turkey; of Horticulture, Faculty of Agriculture, University of Cukurova, TR-01330 Adana Saricam, Turkey; 3Department of Horticulture, Faculty of Agriculture, University of Harran, Sanliurfa, Turkey; 2Department Almond is one of the most important nut crops in Turkey. Genetic linkage maps are important tools to develop markers linked to important phenotypic characters. We constructed SSR-based linkage map in almond using Gulcan-2 x Laurenne F1 population. The consensus map included a total of 168 SSR markers in eight linkage groups (LGs). The number of mapped markers changed from 13 (LG6) to 31 (LG2), and the length of the LGs changed from 47.8 (LG6) cM to 84.6 cM (LG1). Average number of SSR markers per linkage group was 21. The marker density was 3.1 per cM. The linkage map will help to develop markers linked to important traits when phenotyping data was completed. T3-P12 CURRENT STATUS OF REGISTERED POME FRUIT SEEDLINGS IN LEBANON Lamis Chalak, Nagham Tabaja Faculty of Agronomy, The Lebanese University, Dekwaneh, Beirut, Lebanon; Pome fruits represents an important element of the Lebanese agricultural production system while information about seedlings is not yet available in the country. This study presents the first report on the pome fruits seedling status of whether they are locally produced or imported to Lebanon and the resulting impact on the diversity of the Lebanese fruit germplasm. The work was mainly based on an exhaustive desk analysis of the registers of the Ministry of Agriculture over 2012. Data recorded during the three years 144 III International Symposium on Horticulture in Europe – SHE 2016 study indicate an estimated yearly average of 441,520 seedlings registered for apple and pear with around 127,514 imported seedlings (29%) vs. 314,006 locally produced ones (71%). Apple had a yearly average of 95,492 imported seedlings vs. 278,408 locally produced ones. For pear 32,022 seedlings are yearly imported whereas 35,598 are locally produced. At the germplasm diversity level, seedlings of 97 varieties are regularly registered of which 50 varieties are strictly imported for apple and 37 varieties are strictly imported for pear. The remaining 10 varieties were both imported or locally produced. They are: Red Chief, Granny Smith, Golden, Top Red and Royal Gala for apple; and Abate Fetal, Coscia, William, Santa Maria and Conference for pear. Surprisingly, none of the registered seedlings circulating in the country are strictly locally produced while the cultivated pome fruit germplasm is restricted to foreign improved varieties. These data certainly reflect the high diversity level of pome fruit varieties grown in Lebanon but also incite to join UPOV in order to enlarge the spectrum of imported propagating material to the newly released varieties. T3-P13 DETERMINATION THE EFFECTS OF GROWTH REGULATORS ON POLLEN VIABILITY AND GERMINATION LEVEL OF SOME TULIP CULTIVARS Arda Akçal, Βeliha Gökbayrak, (akan Engin Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 Canakkale, Turkey The present study was focused on determining the effects of brassinosteroid, naphthalene acetic acid and gibberellic acid on in vitro pollen germination and pollen viability of some Tulip cultivars. Having knowledge about different sources for pollens must be an important subject for ornamental plant growers or breeders especially in crossing procedure for tulip. Flower bud formation and inflorescence is affected by bulb size, environment, irrigation, nutrition and many other factors. It is also affected by pollen quality such as viability and germination characteristics and besides these they were affected by the cultivars on some ornamental plants. With this point of view, two different tulip variety such as Tulipa greigii 'Pinocchio' and Tulipa Queen of Night , were used as a plant material, pollens collected from both flowers of the cultivars, were tested for their ability to germinate in vitro on a medium (1 % agar and 20 % sucrose) containing three different group of hormones, gibberellic acid (GA3), naphthalene acetic acid (NAA) and epibrassinolide (EBR). After 24 hours of incubation, pollens were evaluated and germination ratios were determined. The results indicate that, pollen germination was significantly affected by growth regulators, also pollen viability and germination rates were change according to the doses and kinds of the growth regulators. The highest average value of the pollen germination rate (%30.2042) was determined in Tulipa greigii 'Pinocchio', while the highest value (4.1563 piece) for the number of pollen germinated was obtained from epibrassinolide (EBR), in comparison to the other applications. T3-P14 DEVELOPING NEW MICROSATELLITE MARKERS IN WALNUT Salih Kafkas, Seckin Karatas, Hayat Topcu Univ of Cukurova Fac of Agriculture, Department of Horticulture, 01330 Adana Balcali, Turkey; We attempted to develop new polymorphic SSR markers in walnut (Juglans regia L.) using sequences from Chandler cultivar in the NCBI database. 200 primer pairs were designed and analysed to characterize 16 walnut cultivars. Of 200 SSR primer pairs, 190 had amplification successfully, and 92 were polymorphic. A total of 324 polymorphic bands were produced from polymorphic loci, and an average of 3.52 alleles per locus was obtained. Polymorphism information content (PIC) was 0.43, the observed (Ho) and expected heterozygosity (He) values were 0.43 and 0.48, respectively. The SSR markers developed in this study may help genetic studies in the genus Juglans. 145 BOOK OF ABSTRACTS T3-P15 DIVERSITY OF CITRUS GERMPLASM IN THE BLACK SEA REGION IN TURKEY Keziban Yazici, Mustafa Akbulut, Burcu Göksu, Nalan Bakoglu Recep Tayyip Erdogan University, Faculty of Agriculture and Natural Science, Horticultural Department, Rize, Turkey. Black Sea agricultural region possess 12,59% of Turkey fruit production and particularly East Black Sea Region ranked as 3rd on citrus fruit production after Mediterranean and Aegean regions. Due to properties of climate, soil and geographical features, main production is focused on hazelnut and tea. In Turkey, citrus fruit production in Mediterranean and Aegean regions is economical because of ecological conditions. However, Satsuma mandarin which has the highest export found its best ecology in East Black Sea Region. In addition to mandarin, local citrus fruit species produced also includes orange, lemon, citron and grapefruit. However, due to increasing rate of tea production in this region, citrus fruit orchards are being destroyed and gene sources are threatening each day. In this paper Citrus Germplasmin East Black Sea region is evaluated and protection approaches are discussed. T3-P16 DYNAMIC PHENOTYPIC GRAPEVINE (VITIS VINIFERA L.) RESPONSES TO CHILLING AND HEATING STRESS Alberto Algarra Alarcón1, Jana Barbro Winkler2, Andrea Ghirardo2, Stella Grando1, Franco Biasioli1, Georg Wohlfahrt3, Jörg-Peter Schnitzler2, Luca Cappellin1 1via Edmund Mach, 1, San Michele all'Adige, Italy; Landstr. , Neuherberg, Germany; 3Sternwartestrasse 15, Innsbruck, Austria. 2)ngolstädter Phenotyping crop cultivars to select those with higher resistance against abiotic stresses is becoming more important due to climate change. Stresses, such as cold, heat, ozone, drought or salinity, can lead to the formation of ROS in the plant tissue, ultimately causing cell damage. Several studies indicate that emission of volatile terpenoid compounds can help mitigating oxidative damage of the photosynthetic system. To date little is known about terpenoid emissions in grapevines under abiotic stress. In this study we investigated the phenotypic differences of four grapevine cultivars (two red and two white) subjected to chilling and heating conditions. Plants were cultivated in gas tight phytotrons. Control d/n temperature conditions were / °C. Plants were chilled for days d/n temperature / °C , followed by days of recovery and, then heated for days d/n temperature / °C followed by a second recovery phase. We used Proton Transfer Reaction Time of Flight – Mass Spectrometry (PTR-ToF-MS) and GC-MS to assess volatile organic compound (VOC) emission and non-destructive photosystem II chlorophyll fluorescence to determine changes in photosynthetic yield due to stress. Combining all data gave a broad picture of the plant dynamic responses to stress. White grape varieties showed an increase of the non-regulated losses of excitation energy under cold conditions, while red varieties emitted more LOX products. Under heat stress, red cultivars showed an increase of the nonphotochemical quenching during heating, while white ones showed a much higher VOC emission, especially on monoterpenes such as cis-ocimene (not emitted by the red). Furthermore, the masses attributed to methanol and the LOX increased 3-5 times during the 1st day of stress, and 2-3 fold during the entire stress period, respectively. These results point out the importance of phenotyping cultivar dependent stress responses and take them into account in future breeding programs. 146 III International Symposium on Horticulture in Europe – SHE 2016 T3-P17 ECTOPIC EXPRESSION OF BRASSICA SERINE-ARGININE PROTEINS, BRA015576 AND BRA018581, INCREASES THERMOTOLERANCE IN ARABIDOPSIS THALIANA Soo In Lee, Eun Kyung Yoon, Jin A Kim, Mi-Jeong Jeong Department of Biotechnology, National Institute of Agricultural Sciences, 54874 Jeonju, Korea (Republic of). Serine-Arginine protein (SR protein) family, which acts as RNA-binding protein, plays a major role in posttranscriptional regulation of pre-mRNA, such as alternative splicing (AS). It is known that SR proteins cause pleiotropic effect by regulating AS of pre-mRNA in tissue and developmental stage specific and stressresponsive manner in Arabidopsis. Here, we identified 30 genes encoding SR proteins in Chinese cabbage (Brassica rapa Chiifu-401) from Brassica database and analyzed their phylogenetic relationship. SR proteins in B. rapa are classified into common (SR, RSZ and SC) and plant specific (SCL, RS2Z, RS and SR-like) subfamilies. To verify the correlation between SR genes and environmental stress, we screened the AS pattern of SR genes to various types of stress, such as heat, osmotic and salinity. In particular, the AS pattern of Bra015576 and Bra018581 were altered under high temperature conditions. In addition, the transcript levels of Bra015576 and Bra018581were induced significantly by heat stress. To investigate whether ectopic expression of Bra015576 and Bra018581would affect heat stress tolerance response of plant, we established transgenic plants expressing constitutively Bra015576 and Bra018581 genes in Arabidopsis, respectively. The transgenic T1 plants showed normal growth under heat-shock conditions °C , whereas wild type plants wilted. These results suggest that the AS regulation by Bra015576 and Bra018581is involved in adaptation mechanism to high temperature stress in Chinese cabbage. T3-P18 EFFECT OF CYTOKININS ON IN VITRO BLASTOGENESIS OF THYMELAEA TARTONRAIRA SSP. TARTONRAIRA (L.) ALL Maria Papafotiou, Aekaterini N. Martini Laboratory Floriculture & Landscape Architecture, Department of Crop Science, Agricultural University of Athens, 75, Iera Odos, 118 55 Athens, Greece Thymelaea tartonraira ssp. tartonraira (L.) All. (Thymelaeaceae) is a much-branched, native dwarf shrub, with numerous silkily hairy leaves and yellowish flowers from February to April. Due to its particular morphology and tolerance to drought and poor rocky soils, it is considered suitable for use as an ornamental landscape plant in xerothermic urban and suburban areas, green roofs and landscape restorations including archaeological sites. In the present study the effect of various cytokinins on in vitro establishment and shoot multiplication was examined in order to find an effective micropropagation protocol, since seed germination is low and stem cuttings fail to root. Shoot tip and nodal explants, excised from in vitro grown seedlings were initially cultured on MS medium either without plant growth regulators or supplemented with . mg/L benzyladenine or . mg/L zeatin. More shoots per explant were produced on a medium with . mg/L . or . mg/L zeatin (3.7) compared to cytokinin-free medium (1.6), while no differences were observed in shooting percentage (> 90%) and shoot length (over 3 cm). Shoots derived from hormone-free medium were subcultured twice on MS medium with 0.5 mg/L zeatin and then became source of nodal explants, which were cultured on MS medium with 0.0, 0.25, 0.5, 1.0 or 2.0 mg/L or zeatin or kinetin or iP. Almost all > % explants produced shoots. The most shoots per explant (7.0-9.5) were formed on media containing 0.25-2.0 mg/L BA or 1.0-2.0 mg/L zeatin. However, at 2.0 mg/L excess callus and hyperhydrated shoots were observed. Shoot length was greatest . -3.3 cm) on media containing kinetin or 0.25 and 1.0 mg/L 2iP or 0.5 mg/L zeatin. Considering explants productivity and shoots normality, at . -0.5 mg/L and zeatin at 1.0-2.0 mg/L were considered the most appropriate for shoot multiplication. NSRF 2007-2013, OPERATIONAL PROGRAMME EDUCATION & LIFELONG LEARNING - THALES ARCHAEOSCAPE. 147 BOOK OF ABSTRACTS T3-P19 EFFECT OF DIFFERENT CARBON SOURCES ON IN VITRO PROPAGATION OF YELLOW PASSION FRUIT Savana Irribarem Costa, Paulo Mello-Farias, Andrio Spiller Copatti, Igor de Albuquerque Federal University of Pelotas, Faculty of Agronomy Eliseu Maciel, Pelotas-RS, Brazil Passion fruit belongs to the genus Passiflora, which includes nearly 400 known species that are mostly found in tropical regions of the American continent, of which 120 are native to Brazil. Under in vitro conditions, plants partially lose autotrophism, requiring an exogenous source of carbohydrates. The objective of this work was to evaluate the effect of distinct carbon sources (sucrose, glucose and lactose) in different concentrations on in vitro propagation of yellow passion fruit. Stem segments with a bud and the excised apex were inoculated on MS medium with addition or no of carbohydrates at concentrations of 0, 30, 59 and 88 mM.L-1, according to the treatment. The experimental design was completely randomized in a 3x4 factorial (source of carbohydrates x carbohydrate concentration), with five replicates, each consisting of a bottle with four explants. After 60 days of cultivation, number of leaves, number of shoots, shoot length, number of roots and root length were recorded. Glucose at a concentration of 88 mM.L-1 induced the highest number of leaves and shoots. Addition of sucrose at 88 mM.L-1 resulted in longer shoots and roots and increased number of roots. T3-P20 EFFECT OF MECHANICAL THINNING ON FRUITLET ABSCISSION AND VEGETATIVE GROWTH IN APPLE Michael Zoth1, Andreas Riehle1, Jens N. Wuensche2 1Kompetenzzentrum 2Emil-Wolff-Str. Obstbau Bodensee, Schuhmacherhof 6, D-88213 Ravensburg, Germany; 25, Crop Physiology of Speciality Crops, University of Hohenheim, D-70599 Stuttgart, Germany; jnwuensche@uni-hohenheim.de In apple orchards, mechanical thinning strategies represent an alternative or supplement to chemical thinning for regulating flower and fruitlet set, hence crop load. In recent years, experimental results suggested a positive effect of mechanical thinning on crop load to achieve commercial fruit size and improved fruit quality. However, the acceptance of mechanical thinning by string machines is relatively low among apple growers. Based on the ethylene-shock-theory , many growers associate a higher flower and fruitlet drop and also an increase in vegetative growth with the use of a string machine. In order to evaluate this general belief and to investigate the underlying physiological mechanisms, fully mature Elstar and Gala trees, both on rootstock M. , were treated with the Darwin string machine Fruit-Tec, Markdorf, Germany) at different spindle speeds (200, 240, 280 and 320 rpm) shortly before full bloom (BBCH 64). Number of flower clusters [prior to thinning (BBCH 59), after thinning (BBCH 65) and after fruit set (BBCH 72)] and total one-year-old shoot length and number of fruit per tree as well as fruit quality parameters on a representative sample at harvest were determinate for each cultivar. In both cultivars a progressive reduction of crop load and increase of fruit quality was achieved with increasing spindle speed. Furthermore, the highest spindle speed reduced the primary spur leaf area per flower cluster up to 35 % in Elstar and 40 % in Gala, respectively. Moreover, both cultivars showed a significantly higher flower and fruitlet drop with increasing spindle speed; however, over-thinning was not induced. Interestingly, also the vegetative growth response of the trees was affected by the thinning treatments. For example, treated Gala trees had up to % more extension shoots (>25 cm) compared to the untreated control. Further research is still needed to explain the physiological mechanisms of the thinning method. 148 III International Symposium on Horticulture in Europe – SHE 2016 T3-P21 EFFECT ON WATER DEFICIT ON TOMATO FRUIT QUALITY - GENETIC VARIABILITY OF THE FRUIT RESPONSE Nadia Bertin, Béatrice Brunel, Julien Bonnefoi, )vana Petrovic, Mathilde Causse 1 UR 1115 PSH, INRA, Domaine St Paul, route de l'aérodrome, Site Agroparc, Avignon, France 2 Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia )NRA, UR , GAFL, Allée des chênes, Domaine Saint Maurice, CS , Avignon, France Water deficit is known to improve fleshy fruit quality at the expense of yield. However in tomato, recent studies showed that such effects are highly dependent on genotypes. Thus, breeding tomato plants adapted to grow under limited water supply requires a better understanding of the effects of WD on fruit quality and their genetic variability. In the present work, four experiments have been performed under greenhouse conditions on the 8 parents of the Multi-Parent Advanced Generation Inter-Cross population which offers the largest allelic variability observed in tomato. Moderate and intensive WDs have been applied during the whole period of fruit development. The experiments were performed either in spring or in autumn to analyze the interactions between WD and season. Moreover, interaction between moderate WD and plant fruit load was measured in spring. Fruit quality was assessed through fresh and dry masses, and fruit composition (soluble sugars, organic acids, vitamin C, carotenoids). Results show that fruit fresh mass is reduced proportionally to WD intensity, whereas the % dry mass is increased. Big fruit size genotypes are much more sensitive to WD than small fruit size genotypes. The improvement of fruit taste, based on the increase of the sugar:acid ratio, resulted more from concentration effects than from metabolic trigger effects. Trade-offs between fruit size decrease and quality improvement allowed discriminating the 8 accessions for their genetic interest under water limited conditions. T3-P22 EVALUATION OF INDIGENOUS FRUITS AND GRAPES GERMPLASM IN THE REPUBLIC OF SRPSKA Gordana Djuric1, Nikola Micic2, Sanda Stanivukovic3, Mirela Kajkut Zeljkovic3, Tatjana Jovanovic Cvetkovic4 1University of Banjaluka, Genetic Resources Institute, Bulevar vojvode Petra Bojovica 1A, 78000 Banjaluka, Bosnia and Herzegovina; 2University of Banja Luka, Banja Luka, Bosnia and Herzegovina; 3UBL, Genetic Resources Institute, Banja Luka, Bosnia and Herzegovina; 4UBL, Faculty of Agriculture, Banja Luka, Bosnia and Herzegovina. In the last thirty years attention has been devoted to the collection, conservation and evaluation of fruit diversity, with two main purposes: to conserve a wide genetic variability for breeding programs and to extend the exploitation of old varieties in organic or integrated fruit production. The area of Republika Srpska (BiH) is very rich in fruit and grape germplasm and it can be considered as a gen-center for some wild fruit species. Inventory, collecting and characterization of fruit and grape germplasm are directed through the National Programme by Working Group for Fruits and Vitis. By the end of 2015 data on 503 accessions in Gene bank are stored: 238 apples, 157 pears, 26 plums, 2 apricot, 30 cherries, 26 figs and 24 grapes. Two collections of fruits are planted in 2013, one in the Botanical Garden of the University of Banja Luka and second in the area of Cajnice municipality (Miljeno). Grape is planted in third collection in Trebinje 2014. A database of the fruits and grapes is made and contains location information, passport descriptors, collection forms, photographs and the results of sanitary status and performed analyzes. For the certain number of inventoried and collected accessions morphological, sensory, pomological, biochemical and molecular analysis were made. 149 BOOK OF ABSTRACTS T3-P23 EVALUATION OF ONION (ALLIUM CEPA. L) BREEDING SELECTIONS FOR RESISTANCE TO DOWNY MILDEW (PERONOSPORA DESTRUCTOR) IN KAZAKHSTAN Bakhytbek Amirov, Zhuldyz-Saule Amirova Kazakh Res. Inst. of Potato and Veg.Growing, Ministry of Agriculture, 1, Nauryz st., v. Kainar, Karasai Distr., Almaty Region, 040917, Kazakhstan. Downy mildew [Peronospora destructor (Berk.) Casp.] is a destructive disease of onion (Allium cepa. L) worldwide. Almost all the onion cultivars released in Kazakhstan are susceptible to Downy mildew (DM), and when the pathogen spread severe plant damages will be significant if no fungicides are used. The objective of this study was to detect sources of resistance to downy mildew among onion breeding selections at the Kazakh Research )nstitute of Potato and Vegetable Growing. Under natural field conditions were evalu‐ated 113 onion entries for downy mildew resistance. Mean ratings for downy mildew leaf damage ranged on a 0 (resistant) to 4 (highly susceptible) scales. Of onion breeding accessions evaluated 19 were categorized as moderately susceptible (rating of 1,1 – 2,0) with a range of diseases spreading from 95 to 100%, and a degree of its development from 30 to 50%. As intermediate susceptible (rating 2,1–3,0) to the diseases were revealed 70 onion accessions with DM lesions, which was distributed to 100% and with a degree of development of 51-74%. The rest 24 onion breeding accessions were highly susceptible (rating 3,1-4,0) to DM, with 100 percent of disease spreading and the degree of development 76-100%. With the least susceptibility were identified onion breeding accessions ON 003, ON 154, ON 157 and ON 367 with a rating scale of 1,2-1,5. The most susceptible onion breeding accessions were ON 295, ON 333 and ON 334 with a rating scale of 4,0. Within the studied entries no onion breeding accession was found to be immune to DM. There were slight correlations between downy mildew leaf damage scale and onion productivity indices (r = -0.332-0.381). However, low levels of susceptibility observed in several onion breeding selections could be useful for the further breeding research for resistance to DM (P. destructor (Berk.) Casp.). T3-P24 EVALUATION OF SOME PEACH CULTIVARS IN THE GENOTYPE COLLECTION OF RESEARCH INSTITUTE OF HORTICULTURE IN SKIERNIEWICE, POLAND Miroslaw Sitarek Research Institute of Horticulture, Konstytucji 3 Maja 1.3, 96-100 Skierniewice, Poland. Peach production in Poland is not as popular as apple production. Poland ranks leading place in the EU in terms of apple production, recently averaging 3.2-3.5 million tons, while 15-25 thousand tons of peaches can be produced in good seasons only. However, average peach production has been increasing in recent years with the renovation of orchards, introduction of new cultivars and the establishment of more efficient planting syste Generally, the climate in Poland is not favourable to peach production. Winters are long and cold, and in the majority of winter seasons temperatures from -15 to -25Co can be expected. In addition, frost/freeze events during blossom in the spring very often limit the fruitlet formation and yielding of peach trees. Therefore, not all peach cultivars grow well and produce good crops in these conditions. To increase orchard productivity and efficiency, fruit growers are looking for solutions mainly in the form of new cultivars that are more resistant to winter cold and spring frost damage, bacterial and fungal cankers, fungal diseases, and they expect first of all very high yields of high quality fruits. The peach field collection in the possession of the Research Institute of Horticulture in Skierniewice, Poland, is not as rich as those in the countries of Southern Europe well-known for peach production. Over 150 cultivars, selections and seedlings of peaches and nectarines are now being evaluated in the collection planted in 1995. The collection is main source of information on peach varieties value for polish fruit growers because no additional experiments in this matter are realized. The paper presents pomological characteristics of high fruit quality foreign cultivars: Maycrest , Manon , (arrow Diamond , Dixired , 150 III International Symposium on Horticulture in Europe – SHE 2016 Early Redhaven , Royal Glory , Diamond Princess , Redhaven , Jerseyland , Flamin Fury P.F. A, Madison , Amgold , Summer Lady , Fidelia , Elberta which on base of multiyear observations well tolerated changeable climate of Poland. Acknowledgement: This work was performed in the frame of multiannual programme on preservation of gene bank resources financed by the Polish Ministry of Agriculture and Rural Development: Task 1.3 Collecting, preservation in ex situ collections, cryoconservation, evaluation, documentation and using of gene bank resources of horticultural crops . T3-P25 EXTERNAL FRUIT QUALITY AND LEAF DISEASE SUSCEPTIBILITY OF FRAGARIA HYBRIDS FROM INTRA- AND INTERSPECIFIC CROSSES Agnieszka Masny, Edward Zurawicz Research Institute of Horticulture, Konstytucji 3 Maja 1.3, 96-100 Skierniewice, Poland. The studies on the phenotypic value of Fragaria hybrids were performed in 2014-2015 at the Research Institute of Horticulture in Skierniewice, Poland. The F1 hybrids were obtained from intra- and interspecific hybridization in early spring of 2014. The field experiment was established in the autumn of 2014. It included more than 6000 seedlings belonging to 57 hybrid families. Fruit quality (fruit size, firmness and appearance) as well as the level of leaf disease severity (leaf spot - Mycosphaerella fragariae, leaf scorch Diplocarpon earliana, and powdery mildew - Sphaerotheca macularis) were evaluated. The most numerous in the population were hybrids with medium-size and large fruits. Only large or very large fruits were produced by hybrids belonging to Chandler x Grandarosa , Konfitura x Grandarosa , Camarosa x Grandarosa and Grandarosa x Pink Rosa . The majority of the hybrids had attractive and very attractive fruits. The most attractive were the fruits derived from Grandarosa x Pink Rosa , Camarosa x Grandarosa , Chandler x Grandarosa , Sophie x Grandarosa and Clery x Pink Rosa . The hybrids of Chandler x Grandarosa , Clery x Pink Rosa and Camarosa x Grandarosa had the firmest fruits. Very few symptoms of leaf spot were observed on the leaves of 20 hybrids only. The remaining seedlings did not show any symptoms of the disease. D. earliana caused very few symptoms of leaf scorch on the majority of the hybrids. Most of the hybrids belonging to Clery x Elsanta and Alice x Grandarosa exhibited no symptoms of the disease. The majority of the hybrids showed no symptoms of powdery mildew. The most resistant to this disease were seedlings derived from Madeleine x Matis , F. chiloensis Del Norte x Matis , Onda x Grandarosa , Camarosa x Pink Rosa , Sophie x Elsanta , F. chiloensis Αaquina B x Grandarosa , F. chiloensis Del Norte x Grandarosa , Spadeka x Grandarosa , Sophie x Grandarosa , Polka x Matis and Onda x Matis . The studies showed that some cultivars, such as Camarosa , Chandler , Clery and Grandarosa , transmit plant tolerance to leaf diseases and good fruit quality, and can be considered good donors of these traits for breeding new strawberry genotypes. T3-P26 FACTORS INFLUENCING MICROPROPAGATION OF GOOSEBERRY (RIBES GROSSULARIA L) Danuta Kucharska,Teresa Orlikowska Konstytucji 3 Maja 13, 96-100 Skierniewice, Poland. The growing interest in the cultivation of gooseberry prompted that in the Research Institute of Horticulture, work was undertaken on development of micropropagation protocols of 15 genotypes of this species. Literature reports on micropropagation of gooseberry are few and they relate to individual genotypes, and indicate a number of difficulties what accounts this species to a group recalcitrant plants in vitro. The main 151 BOOK OF ABSTRACTS problems are: lack of growth and necrosis of explants during the stabilization of cultures, no shoot proliferation or formation of dense rosettes with short shoots not available for further multiplication and rooting. In the first place an impact of type and concentration of cytokinins – kinetin, 6- benzylaminopurine (BAP) and meta-topolin was evaluated. In the result, a negative effect of kinetin and BAP was found. In all studied genotypes, meta-topolin caused an increase in the coefficient of shoots multiplication and for most genotypes a reduction or elimination of dieback that accompanied the presence of BA was observed.Under the influence of meta-topolin a significant increase in number of longer shoots that were able to proliferate as compared with BAP, was obtained. Such improvement of the quality and quantity of the gooseberry cultures using meta-topolin remained constant in the subsequent subcultures. The results indicate on the possible accumulation of BAP or its metabolites in the gooseberry tissues. This detrimental effect of BAP persisted also on the medium without any cytokinin.The other problems to overcome were yellowing of leaves or hyperhydration of shoots. Consequently, another factor studied was the kind of agar. It was stated that Gerlite used for medium solidification caused the occurrence of massive hyperhydration of explants. T3-P27 FLOWERING AND YIELD POTENTIAL OF PROMISING PEACH HYBRIDS FROM CROSSING COMBINATION FLAMINIA X HALE TARDIVA SPADONI Dragan Nikolic, Vera Rakonjac, Aleksandar Radovic Faculty of Agriculture, Nemanjina 6, 11080 Beograd-Zemun, Serbia; In breeding work it is very important to know the flowering characteristics and yield potential of selected genotypes. Prior to the recognition of new cultivars and their introduction into production, it is necessary in addition to many other, carry out testing these characteristics. For this reason, this paper presents the results of two years of research (2011-2012) for flowering phenology (beginning, full and end) and yield potential (initial and final fruit set, fruit weight and yield per 1 m of shoot length) at eight promising peach hybrids obtained from crossing combination Flaminia x Hale Tardiva Spadoni. The flowering of studied hybrids happened from the end of March to mid-April and lasted from 10.0 to 14.0 days. Hybrids FH1, FH4 and FH6 had the earliest and hybrid FH5 had the latest beginning of flowering. The tested hybrids were statistically significantly different in terms of initial and final fruit set and fruit weight. Initial fruit set was very high and ranged from 69.3% in the hybrid FH3 to 97.9% in the hybrid FH7. The hybrid FH7 had the largest final fruit set (52.7%), while the lowest final fruit set had the hybrid FH1 (24.0%). Fruit weight ranged from 97.7 g (hybrid FH6) to 165.6 g (hybrid FH3). The highest yield per 1 m of shoot length were obtained in hybrids FH3 (2.7 kg) and FH7 (2.6 kg) and lowest in hybrids FH5 and FH8 (1.8 kg). Hybrids FH3 and FH7 can be singled out as potentially the most cropping, which makes them interesting for growing in areas where there is a risk of frost. Also, they can be important in breeding programs when creating new peach cultivars of high yield potential. T3-P28 GENETIC RELATIONSHIPS AMONG PISTACIA VERA L. CULTIVARS BASED ON SIMPLE SEQUENCE REPEAT MARKERS Elmira Ziya-Motalebipour1, Salih Kafkas1, Mortaza Khodaijman1, Hatice Gozel2, Nergiz Oban2 Department of Horticulture, Faculty of Agriculture, University of Cukurova, TR-01330 Adana Saricam, Turkey; 2Pistachio Research Institute, Gaziantep, Turkey; Pistacia belongs to the family Anacardiaceae (cashew family), and consists of eleven species. Pistachio (Pistacia vera L.) is the commercially grown species within the genus Pistacia. In this study, phylogenetic relationships among 24 P. vera cultivars were used to test simple sequence repeat (SSR) markers. A total of 206 SSR loci in 24 pistachio cultivars, 897 polymorphic alleles were produced with an average of 4.5 alleles per locus. The effective number of alleles ranged from 1.04 to 7.60. An average of Ho, He and PIC values in P. 152 III International Symposium on Horticulture in Europe – SHE 2016 vera was calculated as 0.46, 0.55 and 0.50, respectively. The constructed unweighted pair group method with arithmetic averages (UPGMA) dendrogram, 24 pistachio cultivars separated into two main clusters: the first one had the cultivars originated from Iran and the second included the cultivars originated from Mediterranean countries such as Turkey, Syria, and Geece. T3-P29 HYBRIDIZATION IN WILD PEAR (PYRUS PYRASTER) FROM VARIOUS REGIONS IN GERMANY AND FROM LUXEMBOURG WITH RESPECT TO PYRUS × COMMUNIS? Iris Wagner 1, Rolf B“ttner 1 Gustav-Adolf-Str. 3, 01219 Dresden, Germany 2 Fruit Gene Bank until 2002, Dresden-Pillnitz, Germany Pyrus pyraster is the prevailing native pear species in Central Europe. The forest tree species settles in a comparatively large area within the temperate zone. Nevertheless, the fruit tree is very rare in most regions of the distribution area and considered to be seriously endangered. Thus, conservation of P. pyraster as part of the natural biodiversity is absolutely essential. The species may serve as genetic resource in breeding programs, e.g. use of resistance towards diseases, drought and air pollution. A precondition for suitable conservation and breeding strategies is the identification of pure wild pears. Introgression from cultivated pears, supposed to be one major threat to the native gene pool, needs to be investigated. )n total, genotypes of P. pyraster and P. × communis were analyzed. The 316 trees initially selected as P. pyraster originated from Germany (242) and Luxembourg (74). Pear trees growing in Germany belonged to populations in the states Rhineland-Palatinate (101), Saxony-Anhalt (120) and Saxony (21). A total of 320 cultivars, separated into old (238) and modern (82) categories, were provided by seven collections in Germany and Oregon/USA (NCGR, Corvallis). Accessions have been typed for eight polymorphic isozyme loci. A model-based Bayesian clustering approach was applied to all multilocus genotype data. Wild and cultivated pear trees made up two different gene pools but could not be discriminated very clearly by nuclear markers. However, modern cultivars appeared to be distinct from all other subgroups. The discrimination between wild and cultivated was less obvious than in European apples. The genetic result might be expected when following the evolutionary hypothesis that P. pyraster is the primary progenitor of European cultivars. Morphologically wild pears from natural sites and old cultivars looked quite different. The extent of hybridization between the two major gene pools investigated is hard to assess. T3-P30 IDENTIFICATION OF POMOLOGICAL CHARACTERISTICS OF SOME IMPORTANT APRICOT VARIETIES GROWN IN TURKEY Mehmet Ali SARIDAS, Sevgi PAYDAS KARGI, Nesibe Ebru Kafkas Department of Horticulture , Faculty of Agriculture, TR-01330 Adana Balcali, Turkey In this study 33 local and foreign apricot genotypes were composed based on their yeild and fruit quality charactristics in Adana ecological conditions. Flowering was observed in Februay and March, and fruits were harvested in May and June. The maturation duration was detected 2.5 months generally. The highest yield/plant were obtained by 55.50 kg (7*89 hybrid no.) and 51.50 kg (3*89 hybrid no.). The yield were changed between 20 to 30 kg in 7 genotypes, 30 to 40 in 6 genotypes. The highest fruit were obtained by Antonio Erranni (91.60 G) and Goldrich (84.77 g) whereas the smallest 2*89 type (30.66g). Fruit firmness were ranged between 1.51 kg/cm2(Aurora) and 7 kg/cm2 (2*89 type). The ratio of fresh/seeds were ranged between 10.53 (2*89 type) to 20.88 (Castelbrite). TSS content of apricot were changed between %6.95 (Canino) to %18.40 (352 type). 153 BOOK OF ABSTRACTS T3-P31 IN SILICO POLYMORPHISM ANALYSIS FOR THE DEVELOPMENT OF SIMPLE SEQUENCE REPEAT MARKERS IN PISTACHIO Mortaza Khodaijman1, Salih Kafkas1, Elmira Ziya-Motalebipour1, Nergiz Coban2, Hatice Gozel2 1Department of Horticulture, Faculty of Agriculture, University of Cukurova, 01330 Adana Saricam, Turkey; 2Pistachio Research Institute, Gaziantep, Turkey Pistacia vera L. is one of the most important nut crops. There are a limited number of simple sequence repeat (SSR) markers in the literature for pistachio. The short Illumina DNA sequences of three cultivars (Siirt, Ohadi and Bagyolu) in P. vera and one genotype belongs to P. atlantica (Pa-Mono-18) were produced by nextgeneration sequencing, and a total of 750 polymorphic loci wre detected in silico and the primers were designed. Of 79 (10.5%) did not have amplification product and they were discarded. The remaining loci were subjected to PCR and then capillary electrophoresis for polymorphism in 18 pistachio female and male cultivars as well as in six P. atlantica genotypes. Fifty-three SSR loci produced complex patterns, and were unable to score. Among the remaining (618) SSR primer pairs, seven had amplifications in two loci, and totally 625 SSR loci were produced from 618 SSR primer pairs. The newly developed novel polymorphic SSRs in this study may help germplasm characterization, genetic diversity, and genetic linkage mapping studies in the genus Pistacia. T3-P32 IN VITRO PROPAGATION OF CITRUS ROOTSTOCKS AND THEIR GENETIC RELATIONSHIPS Konstantinos Salis1, Ioannis Papadakis2, Spyridon Kintzios2, Marianna Hagidimitriou2 2Agricultural 1Ethnikis Antistasis 25, Xylokastro, Greece; University of Athens, Iera Odos 75, 11855 Attiki Athens, Greece; Citrus are amongst the most important commercial group of fruit crops, worldwide. They are being cultivated in more than 100 countries, mostly in warm climates, including the Mediterranean region. In citrus, the choice of the rootstock is essential for the growth, nutrition and longevity of the tree and other characteristics of the scion s performance. The in vitro propagation of seven citrus rootstocks, Volkameriana , Citrumelo Swingle , Citranges Carrizo , Citranges Troyer , Arancio Trifogliato Serra , Ar. Tr. Rubidoux and Ar. Tr. Flying Dragon , was studied for shoot proliferation and rooting. Nodal explants of three months old shoots were used in Murashige and Skoog medium at five different concentrations of N6benzyladenine (BA) (0, 0.5, 1, 2 and 4 ppm) for shoot proliferation and five different concentrations of naphthaleneacetic acid (NAA) (0, 0.5, 1, 2 and 4 ppm) for rooting. The number of shoots, the shoot length and the number of leaves per explant were evaluated at the end of the proliferation phase. The rootstock Volkameriana showed the best results in all concentrations with maximum shoot length at the control ppm BA and the maximum shoot proliferation at ppm BA, while all three Arancio Trifogliato rootstocks showed the lowest numbers. The number of roots and the root length are currently evaluated and will be presented. Additionally, the genetic relationships among the studied rootstocks using molecular markers will be presented. 154 III International Symposium on Horticulture in Europe – SHE 2016 T3-P33 INHERITANCE OF RIPENING TERMS, YIELD AND RESISTANCE TO FUNGAL DISEASES AT THE PEACH Anatoliy Smykov1, Olga Fedorova2 2Nikita 1Crimea, Yalta, Russian Federation; botanical gardens, Yalta, Russian Federation. In our investigations we made hybridologycal analysis of such traits heritability as ripening terms, yield and resistance to fungi diseases for hybrid seedlings of peach in 45 – 55 breeding combinations for determination of economical valuable signs donors and their accessory to particular ecogeographycal groups and ecotypes. Ripening term is an important economical valuable sign determining the period of the fresh peach fruits consumption. On these grounds the breeding was directed at selection of new cultivars with different ripening terms, especially at late - and early- maturing varieties. Researching the ripening term disjoining in the hybrid progeny we could detect that it is being inherited neutral or with inclination toward the one of parents, relevant to their donor features. So we could determine such donors as early (Favorita Morettini, Springtime) and mid-ripening (A. Chekhov, Orpheus, Babygold-5, Vostok-3) cultivars. Gamma-irradiation of seeds at doses of 40, 60 or 80 Gr increased the variability of ripening term of fruits in the hybrid progeny (V = 37.9-66.7 per cent) compared to control (25.7 per cent). The number of early- and early-mid-ripening seedlings has grown up under all irradiation doses. 33.3 per cent of the six early-ripening donors were among the North-Chinese and 77.7 per cent among the Iranian ecogeographycal group. The share of each one ecotype was the same and amounted to 12.6 per cent of donors number. The yield, as well as the ripening term, is a primary economical valuable sign, and this is the reason for hybridologycal analysis of the seedlings generative progeny and for determination of the donors for this sign. Inheritance of yield proved to be neutral or with inclination toward the one of parents, relevant to their donor features. We selected the following yield donors: A. Chekhov, Tovarisch, Favorita Morettini, Orpheus, NektadianaI1 26-76, Silver Profilic, Podarok Krima (open pollination), Spartak, Sochniy. 70 per cent of yield donors were among the North-Chinese and 30 per cent among the Iranian ecogeographycal group. The most donors belonged to the European ecotype of the North-Chinese group (60 per cent). The hybrid progeny evaluation must be complemented by hybridologycal analysis with the aim of serching donors of enhanced resistance to fungi diseases, such as leaf curl and powdery mildew. Hybridologycal analysis allowed us to detect following donors of high resistance to powdery mildew: the cultivars Silver Profilic, Veteran, Vostok 3, Zolotaya Moskva and the form Podarok Krima op. pol.. Silver Profilic and Padarok Krima op. pol. were marked as complex donors of enhanced resistance to leaf curl and powdery mildew. All five donors of high resistance to powdery mildew were among the North-Chinese ecogeographycal group. The most donors belonged to the European ecotype of North-Chinese gpoup (60 per cent). T3-P34 INTROGRESSION OF AGRICULTURAL TRAITS AND RESISTANCE FROM ASPARAGUS WILD RELATIVES INTO THE GARDEN ASPARAGUS (ASPARAGUS OFFICINALIS L.) Reiner Kramer, Thomas Nothnagel Fed. Centre for Breeding Res on Cult. Plant, Erwi-Baur-Strasse27, 06484 Quedlinburg, Germany Garden asparagus (Asparagus officinalis L.) is one of the most popular vegetables worldwide with increasing importance. Limiting factors for the increase of production and the quality are a large number of pests and diseases as well as environmental factors such as salinity of soils, drought, wind or temperature stress. Extensive investigations have shown that nearly all asparagus fields in Europe are infested with different viruses, especially with the Asparagus virus 1 (AV-1). Crown and root rot caused by Fusarium spp. and some other fungal diseases caused by Puccinia, Stemphylium, Phomopsis or Phythophtora as well as abiotic factors probably resulted in browning of spears, became more and more important. The genetic diversity of A. officinalis is relatively low and resistance against biotic and abiotic stress not yet available. In contrast, approximately ten Asparagus species have been identified as disease resistant, but the most of the more than 155 BOOK OF ABSTRACTS 200 species mainly distributed in Africa, Asia and Europe have not yet been analysed. Focus of our research is i) the systematic evaluation of a large number of Asparagus wild relatives for agronomical traits, resistance to the AV-1 as well as Fusarium spp. and ii) the introgression of the interesting traits into A. officinalis. Preliminary results of our evaluation approaches and two backcross programs aiming to introgress resistance to the AV-1 from wild relatives into A. officinalis will be presented. T3-P35 INVESTIGATIONS ON THE FORMATION OF DIHYDROCHALCONES IN THE MODEL PLANT APPLE (MALUS SP.) Malvina Milosevic 1, Annette Rompel 1, Christian Gosch 2, Heidi Halbwirth 2, Andreas Spornberger 3, Karl Stich 2, 1 University of Vienna, Department of Biophysical Chemistry, Althanstr. 14, 1090 Vienna, Austria 2 Technical University of Vienna, Institute of Chemical Engineering, Getreidemarkt 9 1665, 1060 Vienna, Austria 3 BOKU, Division of Viticulture and Pomology, Konrad Lorenz-Strasse 24, 3430 Tulln an der Donau, Austria The apple tree (Malus sp.) accumulates high amounts of the dihydrochalcone phloretin and its derivatives, whilst few other species contain these compounds only in low amounts. Whereas p-coumaroyl-CoA is the precursor for the naringenin chalcone and further flavonoid formation, p-dihydrocoumaroyl-CoA is required for the biosynthesis of dihydrochalcones. The formation of p-dihydrocoumaroyl-CoA from p-coumaroyl-CoA in apple is characterized at the enzymatic level whereas the corresponding gene is not unequivocally found. We use two approaches to elucidate the identity of the gene responsible for p-dihydrocoumaroyl-CoA formation: Potential candidate genes including an enoyl-ACP reductase, acyl-CoA dehydrogenase and three different double bond reductases were cloned into the expression vector pGEX-6P-1 and transformed in E. coli. For purification purpose the recombinant proteins were expressed as GST fusion proteins. Activity of the heterologous proteins was tested with p-coumaroyl-CoA as substrate and subsequent evaluation of phloretin and naringenin formation after further enzymatic conversion with chalcone synthase/chalcone isomerase in the presence of malonyl-CoA. In parallel, purification of the enzyme responsible for the formation of p-dihydrocoumaroyl-CoA from apple leaves is in progress to allow cloning of the respective gene via the deduced DNA sequence corresponding to the purified protein sequence. Additionally we describe that the hydroxylation of phloretin in position 3 has high similarity to the B-ring hydroxylation of flavonoids catalysed by the well-known flavonoid -hydroxylase F ( . Using recombinant F ( and chalcone -hydroxylase C( ( from Cosmos sulphureus we show that F ( and C( ( accept phloretin to some extent but higher conversion rates are obtained with CH3H. T3-P36 'LEMONATO' PEACH: A SERIES OF CULTIVATED CLONES WITH HIGH FRUIT QUALITY George Nanos1, Nikoleta Mitsopoulou1, Ekaterini Grigoriadou2, Nikolaos Katis3 1Lab. of Pomology, Sch. of Agricultural Sci., University of Thessaly, Fitoko Str, 38446 Volos, Greece; 2Vitro Hellas S.A., 59300 Alexandria Imathias, Greece; 3Lab. Plant Pathology, Sch. of Agriculture, Aristotelean Univ. of Thessaloniki, 54124 Thessaloniki, Greece; The Lemonato peach is a series of clones well known in Magnesia, Central Greece. These clones were selected locally from sporophytic populations for different time of ripening and different fruit quality traits. The fruits are phenotypically discriminated from the other peach cultivars, as they have typical yellow skin colour when ripe, very little red hue and unique flavour and taste. The scope of the 2-year present work was the description of the three well-distinguished (due to ripening time) clones based on morphological and agronomic plant characteristics, and fruit quality characteristics. Five trees of each Lemonato clone in 156 III International Symposium on Horticulture in Europe – SHE 2016 different locations throughout the cultivation area were selected based on ripening time. Presence of major stone fruit viruses was conducted for each tree. For the morphological description of each clone, flowering time, shoot and fruit growth were followed in the field and shoots and fruit were described by UPOV. Specific leaf weight and leaf chlorophyll concentration were measured in late August. Fruit quality traits were also studied including fruit weight, skin colour, fruit firmness, soluble solids content, titratable acidity, total phenols and antioxidant activity. Obtained results showed significant variation per clone and cultivation area for physiological leaf characteristics, shoot development, blooming time initiation, maturation time and fruit quality. Trees with the best characteristics from each clone were separated to initiate organized propagation. In the near future, virus-free basic propagation material for each selection with different ripening time and experimental farms set up will be implemented to exploit useful genetic traits through breeding programs. These actions will help spread the cultivation of this indigenous fruit clone series. T3-P37 MICROPROPOGATION OF SIDERITIS PISIDICA BOISS. ET HEL APUD BENTHAM Basar Sevindik1, Tolga Izgu2, Mehmet Tutuncu3, Ehsan Mohammad Tagipur4, Pembe Curuk1, Ozer Yilmaz5, Gonul Kaynak5, Yesim Yalcin Mendi1 1University of Cukurova, Faculty of , Agriculture, Department of Horticulture, Balcali, 01330 Adana, Turkey; of 304zmir Faculty of , Agriculture Department of Horticulture, Plant Biotechnology Lab., 35100 Bornova Izmir, Turkey; 3University of Samsun Ondokuz Mayis, Faculty, of Horticulture, Atakum, Samsun, Turkey; 4University of Cukurova, Institute of Natural and Applied Sciences, Department of Biotechnology, Balcal305, 01330 Adana, Turkey; 5University of Uludag, Faculty of Arts and , Science, Department of Biology, Nilufer, 16285 Bursa, Turkey; 2University Sideritis pisidica Boiss. Et Hel Apud Bentham is known by medicinal and aromatic plant. Sideritis pisidica Boiss. Et Hel Apud Bentham is a plant which is endemic to Turkey and under threated species in the Labiateae family. This species is used in folk medicine for the ancient time. Micropropogation is an alternative method for rapid clonal propogation. In this study, Sideritis pisidica Boiss. Et Hel Apud Bentham were micropropagated using MS medium containing different combination of plant growth regulators; BA (0, 0.5, 1 and 1.5 mgL-1) and GA3 (0, 0.5, 1 and 1.5 mgL-1) for propagation, IBA (Indole-3butyric acid) 0, 0.5, 1, 1.5 and 2 mgL-1) for rooting. The MS medium supplemented with 0,5 mgL-1 BA and 0,2 mgL-1 GA3 (Giberllic acid) showed highest percantage of shoot multiplication and rooted with %55 and %20 response from shoot tip explant respectively. T3-P38 MOLECULAR CHARACTERIZATION OF CYST FORMING NEMATODES DETECTED ON CHINESE CABBAGE IN GANGWON, KOREA Hyoung-Rai Ko, Min-A Lee, Eun-Hwa Kim, Jae-Kook Lee Wanju 55365, Wanju, Korea (Republic of) In 2011, The damages of chinese cabbage were occurred by cyst forming nematode in Taebaek, Korea, and It were spilled over into nearby area, Jung-seon and Sam-cheok. However, It seems to be the cyst forming nematode that damages on chinese cabbage is not one species. Therefore, in this study restriction fragment length polymorphism (RFLP) and molecular phylogenetic analysis were conducted to identify the cyst forming nematodes that detected in chinese cabbage fields, located in Sam-cheok. The three types of RFLP pattern were showed on electrophoresis gel when RsaI and MvaI were treated to PCR products of ITS rRNA region. Phylogenetic analysis of mitochondrial DNA, cytochrome c oxidase subunit I (COI), showed that three types of cyst forming nematode were present. It is expected that there are three species of nematode, Heterodera schachtii, H. glycines and Heterodera sp., in Sam-cheok, Korea according to this research. It's 157 BOOK OF ABSTRACTS necessary to be identified the mophological characterization of three nematodes for accurate identification in succeeding study. T3-P39 NEW CULTIVARS OF HIGH QUALITY NECTARINES AT THE CRIMEA Sergey Tsiupka1, Evgeny Shoferistov2, Iuliia Ivashchenko2, 2Nikitsky 1Crimea, Yalta, Russian Federation; Botanical Gardens, Yalta, Russian Federation. In the Crimea the nectarines breeding is concentrated at the Nikitsky Botanical Gardens in Yalta. In 1938 Nikitsky Botanical Garden initiated a nectarine breeding program in order to select new genotypes suitable for the fruit growing areas of the Crimea. These regions are generally characterized by shot, warm winter and long hot dry summer. The Crimea is rich in nectarine genetic resources, with very different characteristics such as time of bloom, early and late ripening and variable fruit quality. The selection of new cultivars is focused on fruit quality, high and stable fruit set and tolerance to biotic and abiotic stress. More than 2000 nectarine hybrids obtained at the Nikitsky Botanical Gardens, the Crimea (Russian Federation) were tested in 1980-2015. About 60 different nectarine cultivars with different fruit characteristics (size, skin color and overcolor, flash firmness, sugar content, titratable acidity, absence of split pit) were used as parental. As a result of the breeding activities three new nectarines cultivars Krymchanin , Rubinovy- and Rubinovy- have been selected. We used the Le Grand cultivar as mother plant and the hybrid 68-72 as pollen donor plant in our breeding programme, and obtained the Krymchanin cultivar. Rubinovy- was selected from seedlings of free-polination peach cultivar Kentavr. Cultivar Rubinovy- was selected from seedlings of free-pollination nectarine cultivar Claudia . Fruits flash of these cultivars is yellow. The flavor is sub-acidic, sweet and aromatic. The fruits of these cultivars showed good transportability. Resistance to the most important fungal diseases is equal or higher than Super Crimson Gold cultivar. The trees were grafted on GF 305 and GF 667 rootstock, Amygdalus communis L., Prunus cerasifera Ehrd. and planted at the distance 4,0x3,0 m. The trail was established in five replications (1 tree per replication). As a result of our breeding programme the new nectarine cultivars Krymchanin , Rubinovy- and Rubinovywere handed for state registration. Acknowledgement: This study was funded by a research grant № Foundation. -50-00079 of the Russian Science T3-P40 NEW DWARF BEAN VARIETIES OBTAINED IN ROMANIA Eliza Teodorescu, Floarea Burnichi, Petre Constantin Mesteacanului Street, No 23, 120024 Buzau, Romania; One of the main objectives concerning the research activity of the Vegetable Research and Development Station (V.R.D.S.) Buzau collective is represented by biodiversity preservation at the vegetables species and also the valorisation of the germplasm collection by creating new varieties. The paper presents the main characteristics of 10 local populations of dwarf bean (Phaseolus vulgaris L. convar. nanus) selected from this species germplasm collection that contains over 150 accessions. In 2014 weather conditions, the most productive variant was V2 having 291.57 g/plant. At the same variant there were registered maximum values regarding thickness (1.2 cm), weight (13.25 g) and the proportion between pods thickness and width (1.14). The pods obtained from V2 variant are green having a circular or eight shaped cross section and it does not form stringiness on ventral suture at the physiological maturity. In what it concerns V9 variant there were registered on the one hand the highest value regarding the number of pods/plant (27.33) and the number of seeds/pod (7), and on the other hand there are registered the lowest values regarding width (0.84 cm), thickness (0.73 cm) and pods weight (6.83 g). These characteristics confer an increased commercial value to V9 variant. After 52 days since seeding V10 had pods with 12 cm length, and at V7 the pods 158 III International Symposium on Horticulture in Europe – SHE 2016 measured 9 cm. Due to the special characteristics (earliness, productivity, distinctivity, uniformity, stability) a variant has been homologated (V9 – Clarisa), another variant is in process of homologation (V2 – Cristiana), and others will be homologated as new varieties. T3-P41 OLD IS BETTER: A MULTIPLE APPROACH TO IDENTIFY HYPOALLERGENIC APPLE GENOTYPES Mara Vegro 1, Giulia Eccher 1, Francesca Populin 1, Federica Savazzini 2, Giulia Pagliarani 2, Stefano Tartarini 2, Gabriella Pasini 1, Andrea Curioni 1, Andrea Antico 3, Alessandro Botton 1, 1 University of Padova - DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly 2 DSA - University of Bologna, Viale Fanin, 46, 40127 Bologna, Italy 3 Allergy Unit, Ospedale Civile Srl, 46049 Volta Mantovana(Mantova), Italy Freshly consumed apples (Malus domestica L. Borkh) can cause allergic reactions because of the presence of four classes of allergens, namely, Mal d 1, Mal d 2, Mal d 3, and Mal d 4. Knowledge of the genetic factors affecting the allergenic potential of apples would provide important information to apple breeders, growers, and consumers for the selection of hypoallergenic genotypes combined with the adoption of agronomical practices decreasing the allergenic potential, and the consumption of fruits with reduced amount of allergens. In the present research, a multiple analytical approach was adopted in order to characterize the allergenic potential of 24 apple varieties including both pre- and post-green revolution genotypes. A specific pipeline was set up including gene expression assays, protein quantification by means of specific antibodies, immunological analyses with sera of allergic subjects, enzymatic assays and clinical assessments on allergic patients. Taken as a whole, the results indicate that most of the less allergenic genotypes were found among those deriving from selection processes carried out prior to the so called green revolution . Among these old genotypes, some were good candidate hypoallergenic varieties, showing very promising results not only in vitro but also in vivo, when these apples were tested with prick-by-prick directly on allergic patients. The unequivocal indications given by this study open the actual possibility of searching for hypoallergenic apples among those already available, thus eventually avoiding transgenic solutions that would not be easily accepted by consumers. T3-P42 OMIC APPROACHES TO SHED LIGHT ON APPLE FRUIT DEVELOPMENT Francesca Populin 1, Giulia Eccher 1, Mirko Moser 2, Sergi Munne-Bosch 3, Javier Miret 3, Annick Moing , Mickaël Maucourt , Sylvain Guyot , Agnes Gacel , Duyen Prodhomme 4, Elisa Asquini 2, Azeddine Si-Ammour 2, Alessandro Botton 1 1 University of Padova - DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly 2 FEM, Via E. Mach, 1, 38010 San Michele all'Adige(Trento), Italy 3 Dept. of Plant Biology, Universitat de Barcelona, Barcelona, Spain 4 INRA, UMR1332 Biologie du Fruit et Pathologie, 71 av Edouard Bourlaux, Villenave dOrnon, France 5 INRA, MetaboHUB, IBVM, 71 av Edouard Bourlaux, Villenave dOrnon, France 6 Chargé de Recherches, Equipe PRP, Domaine de la Motte BP , Le Rheu, France Apple (Malus domestica L. Borkh) is one of the most economically relevant fruit crops in the world, and Italy is among the most important apple producers. Nowadays the scientific interest on this species is growing exponentially, from the point of view of both basic and applied research. Apple is being increasingly considered as a model species for studying fruit development and many other related process, like i.e. the ripening syndrome. Despite the availability of large collections of biological data, which are frequently focused either on early development (cell division) or on the ripening phase, only a few information are available for what concerns the intermediate stages (cell expansion and maturation) and the transition between different phases of fruit development. Furthermore, this information was collected in different years and from diverse genotypes, and sometimes with contradictory results. For these reasons, omics 159 BOOK OF ABSTRACTS approaches have been used to shed light on the regulatory network of apple fruit development (cv. Golden delicious) along the whole fruit developmental cycle, including both the main phases (cell division, cell expansion, maturation and ripening) and the transitions. Sample collection started from fruit set (immediately after pollination) up to the ripening in planta. Transcriptional, metabolomic and hormonal profiling analyses were performed and results integrated through a dedicated bioinformatic pipeline. Correlative networks were built as a starting point to understand the molecular and metabolic events governing the apple fruit development. T3-P43 PHENOLOGICAL CHARACTERIZATION AND WATER CONTENT OF 'HOUSUI' PEAR TREES UNDER MILD WINTER CONDITIONS Jones Eloy , Maurício Gonçalves Bilharva , Bruno Carra , Priscilia L’cio , Chaiane Milech , Jessica Gonsalez Cruz , Rômulo Perez , Robson Ryu Αamamoto , Ana L’cia Soares Chaves2, Paulo Mello-Farias1, Flavio Gilberto Herter1 1 Federal University of Pelotas, Faculty of Agronomy Eliseu Maciel, Pelotas-RS, Brazil 2 Federal University of Pelotas, Biochemistry - CCQFA, Pelotas-RS, Brazil It is extremely important to determine phenological cycle and water dynamics in pear branches and buds under mild winter conditions, because determining flower primordia necrosis may elucidate this adaptation problem and can be an indicator as a mark of endodormency end. This study aimed to characterize the phenological cycle and evaluate water content WC percentage in different tissues and portions of (ousui pear trees. The experiment was conducted in the orchard at Federal University of Pelotas, RS, Brazil. Five pear trees were selected, and for each plant a branch of each quadrant (north, south, east and west) was researched. Different parts of the plant (basal, median and apical), adding to 12 branches in each one of the five plants, were observed coming into a total of 60 branches. Phenological stages 'C' (green tips) and 'D' (half-inch green leafless) were used as reference and assessments lasted 35 days, from August 26 to September 30, 2015. Three tissues were measured (bud, bark and wood) and three portions of the plant were sampled three times (August 28, September 08 and 18) during winter season of 2015. It was observed buds in C and C3 phenological stages in all quadrants and portions of the plant. Median portions of north, south and west as well as north apical showed greater decrease of C buds. There was no significant interaction between the factors time and plant tissue portion for WC value. The only statistical difference was observed between different types of tissues related to WC rates. In all samples, bark tissues showed the highest values for WC percentage when compared to bud and wood, with decreasing trend from the second collection. The lower WC index is related to lack of cold in the trial implementation period, which resulted in high rates of floral bud abortion. T3-P44 POLYMORPHIC EXPRESSED SEQUENCE TAG-SIMPLE SEQUENCE REPEAT (ESTSSR) MARKERS IN APRICOT Kahraman G“rcan, Muhammet Ali Köse Erciyes Üniversitesi, Genome and Stem Cell Center, Kayseri Talas, Turkey; The apricot (Prunus armeniaca L.) is a member of the subfamily Amygdaloideae (Prunoideae) within the family Rosacea. It is grown commercially worldwide in all temperate regions. Breeding of apricot s requires identification of polymorphic loci such as Simple Sequence Repeat which is particularly useful in understanding molecular mechanism of agronomic traits when identified in genic regions of the genomes. In this study, expressed sequence tags (ESTs) were generated from six apricot leaves and assembling the ESTs revealed 6.359 genes longer than 3.000 nucleotides. Their metabolic pathways around 6.000 were identified. Plenty of SSR loci reaching up to the number 24,635 were found screening transcriptomes. Primers pair of 200 SSR loci were designed and amplified using DNA samples of diverse 24 accessions. Seventy tree of the 160 III International Symposium on Horticulture in Europe – SHE 2016 total were found to be polymorphic. The number of alleles per locus ranged from two to 9, with a mean of 5.45. Mean values for expected heterozygosity, observed heterozygosity, and polymorphism information content were found, averaging 0.59, 0.62, and 0.63, respectively. The new genic polymorphic SSR will be useful in apricot breeding programs. T3-P45 PROTEOLYTIC ACTIVITY, IDENTIFICATION AND EXPRESSION PROFILE OF A KDEL-CYSEP GENE IN COMMON LILAC FLOWERS DEPENDING ON THE FLOWERING CONDITIONS Agata Jedrzejuk1, Aleksandra Lukaszewska1, Margrethe Serek2 1Department 2Leibniz of Ornamental Plants, Warsaw University of Life Sciences, Nowoursynowska 166, 02-787 Warsaw, Poland; University of (annover, Faculty of Natural Sciences, Floriculture, (errenhäuser Str. 2, 30419 Hanover, Germany. In a temperate zone, common lilac blooms in May, and the whole flowering process lasts ca. 3 weeks. The flowering process starts in July of the previous year, during winter flower buds are in the state of deep dormancy, and their activity starts again in the spring of the next year. During winter, flower buds of lilac are in a deep dormancy state, which may be released by cold (usually 260 – 1000 hours of temperature below ºC . Forcing is a method, that may induce flowering in plants, independently of the natural blooming period. The temperatures required to start blooming in lilac during standard forcing method - in November are ca. °. This method is rapid, but gives panicles of poor quality. The alternative is forcing at °C, but in November plants forced by this method require 49 days to bloom as compared to days for the standard °C. Anyway, the main advantage of this method is a very good quality of flowers and their long vase life. In this study we have investigated the protein content, total protease activity, and cysteine protease activity at different stages of flowering (flower bud whitening and swelling, open flowers, wilted flowers) for lilac flowers (Syringa vulgaris L., fam. Oleaceae) blooming under three different conditions: natural conditions in May and forcing in a greenhouse in November at 37oC (standard forcing) or at 15oC (alternative forcing). The higher soluble protein content and the lowest proteolytic activity had flowers from shrubs forced by alternative method ºC . The partial coding sequence for a KDEL-CysEP was isolated, and its expression was determined by qRT-PCR. The gene expression did not correlate with the cysteine endoprotease activity. Alternative forcing method at °C affected the flowering process delaying senescence, presumably due to the low cysteine protease activity. T3-P46 REGULATION OF FLAVONOID BIOSYNTHESIS IN ARTICHOKE: ISOLATION AND CHARACTERIZATION OF A MYB TRANSCRIPTION FACTOR Emanuela Blanco 1, Wilma Sabetta 1, Anita Morgese 1, Donatella Negro 1, De Lisi Antonino 1, Giulio Sarli 1, Francesco Paolocci 2, Gabriella Sonnante 3 1 Via Amendola 165A, 70126 Bari, Italy 2 Via Madonna Alta, 130 , 06128 Perugia, Italy 3 C.N.R.-Istituto di Bioscienze e BioRisorse, (IBBR), Via Amendola, 165/A, 70126 Bari, Italy Phenolic compounds play central roles in plant biology, as they are involved in the protection against various biotic and abiotic stresses, in the regulation of plant reproduction and development, and they act as signalling molecules. Moreover, they exhibit health promoting effects when consumed in food of plant origin. Globe artichoke (Cynara cardunculus var. scolymus) represent an important component of the Mediterranean diet, as it is a natural functional food rich in bioactive phenolic compounds, inulin, fibre and minerals. 161 BOOK OF ABSTRACTS To study the regulation of flavonoid biosynthesis, an R2R3-MYB transcription factor, CcMYB12, was identified from artichoke leaves. Phylogenetic analysis showed that this proteins belong to the subgroup 7 clade (flavonol-specific MYB), which includes Arabidopsis AtMYB12. Heterologous expression in bacteria to study protein/DNA interactions and quantitative Real-time PCR were performed to gain insights into the molecular mechanism of polyphenols regulation in artichoke. Arabidopsis and tobacco plants overexpressing CcMYB12 protein were generated and characterized. Gene expression analyses and HPLC polyphenols quantification were performed on overexpressing lines to study CcMYB12 function in vivo. The present study could improve our understanding of phenylpropanoid biosynthesis and its regulation, to develop agronomic, genetic or biotechnological tools to enhance the levels of these bioactive molecules in artichoke. T3-P47 RNASEQ ANALYSIS OF GRAPEVINE AND BOTRYTIS CINEREA GENE EXPRESSION DURING LATENT INFECTION OF BERRIES ("NOBLE ROT") Arianna Lovato, Teresa Colombo, Gianbattista Tornielli, Annalisa Polverari Strada Le Grazie 15, 37134 Verona, Italy. High throughput technologies provide a unique opportunity to deeply investigate the molecular mechanisms involved in plant-pathogen interaction. Botrytis cinerea, is the agent of grapevine grey mould, but in yet uncharacterized environmental conditions, a latent infection can occur determining favourable berry modifications possibly contributing to the typical aromas of passito wines noble rot .The present project aims at the identification of the specific gene sets deployed by B. cinerea during the latent infection of grape berries and the subsequent V. vinifera responses.Healthy Garganega berries have been artificially inoculated with B. cinerea B05.10 by injecting conidia under berry skin and placed in controlled conditions to reproduce the pourri plein stage of noble rot. Biological triplicate of berry samples inoculated with water and in vitrogrown B. cinerea mycelium were used as controls.Samples were analysed at a transcriptomic and metabolomic level by RNAseq (Illumina-HiSeq500) and HPLC (High-Performance Liquid Chromatography technology), respectively. The RNA-sequencing experiment yielded a total of 533.779.730 raw sequences. Overall, the 93,5% of total reads passed quality control filtering and the 78% of these reads were properly aligned against the Botrytis cinerea and/or grapevine genome. Differential expression analyses revealed 2503 and 2871 differentially expressed genes (False Discovery Rate1.8) in grapevine and B. cinerea, respectively. In response to noble rot grapevine activated 1738 genes principally involved in response to stress, transcription regulation and secondary metabolism (phenylpropanoid, flavonoid and lignin pathways) probably deployed to stop fungal infection. Genes related to cell death, defence responses and transport were found instead repressed as well as terpenoid and auxin pathways, which are instead reported to be induced. The integrative analyses of transcriptomic and metabolomic data and the deep investigation on B. cinerea transcriptome reprogramming during noble rot will be presented. T3-P48 SEED GERMINATION AND SEEDLING SURVIVAL OF CAROB (CERATONIA SILIQUA) UNDER NATURAL CONDITIONS 1 Recep Balkic1 , Sadettin G“ler2, (amide G“bb“k1 Akdeniz University Faculty of Agriculture, Department of Horticulture, Antalya, Turkey 2 Southwest Anatolia Forest Research, Institute SAFRI, Antalya, Turkey Carob tree grown in Mediterranean climate is resistance to drought and fire and it is use for fire protection line has been increased in recent years in Turkey. Therefore, it is important to know seed germination and seedling survival for plantations established using seed material as plant source and marginal lands where carob seeds are planned to be used as plant source. Thus, this study had been set to test seeding time 162 III International Symposium on Horticulture in Europe – SHE 2016 (November and January) on treated seeds with two different acids and control treatment in two different marginal lands (light and heavy rocky texture). Germination time, rate and speed (germination energy) and survival rate of seedlings were evaluated. While seeding time had no effect, treatments and land type had affected on the measures. Germination rate was lowest on control with 7.8 % and highest on acid treated one with 34.7% (30 min H2SO4 followed 24 h water soaked). Germination energy, a measurement to have an idea on vigor of seeds and on the seedlings was between 16.3 - 60.6% and germination lengths among treatments were in between 45.6 – 62.5 days. Seeds sown in marginal land with light rocky texture germinated earlier compared to seeds sown in marginal land with heavy rocky texture. Similarly germination energy was also higher in seeds sown in marginal land with light rocky texture compared to seeds sown in the other soil. It is observed that summer heat was very harmful on all seedlings regardless of treatments. Thus, summer irrigation has of importance to have stayed alive seedlings. T3-P49 SEED GERMINATION STUDIES ON GUAVA (PSIDIUM GUAJAVA) Nafiye Adak1, Ilhami Tozlu2, Recep Balkic2, Lokman Altinkaya2, Ahmet Soydal2, Hamide Gubbuk2 1Akdeniz 2Akdeniz University, Technical Sciences, Vocational Higher School, 07058 Antalya, Turkey; University, Faculty of Agriculture, Department of Horticulture, 07058 Antalya, Turkey Propagation from seed cannot maintain the genetic purity and results in large variation because of sexual reproduction. Therefore, propagation through seed, is preferred for breeding and genetic studies but not for commercial orchard establishments. However, guava varieties are rather difficult to propagate by usual vegetative methods employed for other fruits. It is difficult to obtain a high percentage to alive and these methods are also rather slow since a period of two to three years is necessary from the time seed is planted to produce a salable tree. Even though, seedlings cannot be relied upon to produce fruit identical with that of the parent tree, the common guava usually is propagated by seed. Seed treatments are important to increase germination rate and to obtain homogeny on seedlings. For these reasons, eight different pretreatments were carried on seeds of Rubre Supreme guava variety to evaluate their effect on germination time, rate and speed (germination energy) and chlorophyll index value in greenhouse conditions. Pretreatments were including; (1), control, different time of incubations in; (2) 1MPa PEG 6000 (three days), (3) 100ppm GA3 (60 minutes , °C water minutes , pure sulfuric acid minutes , sulfuric acid minutes + 1MPa PEG 6000 (three days), (7) 100ppm GA3 (60 minutes)+ 1MPa PEG 6000 (three days) and (8) pure sulfuric acid (10 minutes) + 100ppm GA3 (60 minutes). All the measures were found to be important except measures on chlorophyll index values of germinated seedlings. Germination rate were between 30 % (control) – 100 % (Treatment 5). Germination period were between 23.6 days (treatment 5) -46.2 days (control). The best germination energy was calculated on treatment 4 and 5. Looking at all treatments, best overall performance was obtained treatment 5 and may be recommended to be used for guava seed pretreatment. T3-P50 SPECIFIC STRESS-RESPONSES OF MALUS UNDER REPLANT CONDITIONS AS AN INDICATOR FOR THE DEFINITION OF RESISTANT/TOLERANT APPLE ROOTSTOCKS Sonja L“tzenkirchen, Joana Henfrey2, Michaela Schmitz Von-Liebig-Str. 20, 53359 Rheinbach, Germany; 2Auf dem (“gel , Bonn, Germany. Apple replant disease (ARD) leads to reduced yield and quality in commercial apple production all over the world after repeated growing of apple trees on the same area. Symptoms are damaged roots, resulting in less growth and vigor of the plants. Stress responses caused by ARD contaminated soil can provide an insight into correlations of the disease as stress responses of biotic and abiotic factors. Different rootstocks of Malling 163 BOOK OF ABSTRACTS series and Geneva series were planted on untreated replant soil and soil treated at °C and were analysed under vegetative and biochemical aspects. Used methods were the measurement of antioxidative potential and determination of the spectra of polyphenols in apple rootstocks. It was shown that biochemical response to ARD can be detected after 4 weeks, while vegetative response is significant after 8 weeks. The response of apple rootstocks to ARD could be used in future breeding program. T3-P51 STRUCTURAL AND FUNCTIONAL FEATURES OF LEAVES IN SOME FICUS CARICA L. CULTIVARS IN SITU AND IN VITRO Irina Mitrofanova, Valentina Brailko, Olga Mitrofanova Nikita Botanical Gardens, National Scientific Center, Nikita, 298648, Yalta, Russian Federation The results of morphology, anatomy, photosynthetic activity and water regime studies in common fig (Ficus carica L.) cultivars` leaves with and without symptoms of fig mosaic disease (FMD), growing in the collection plots of Nikita Botanical Gardens are presented. )n common fig cultivar Medovyiy with FMD symptoms significant decrease in linear dimensions of leaves compared with symptomless plants was noticed. Reliable differences in adaxial leaf epidermis thickness in the cultivars 'Medovyiy' and 'Smena' were identified. Index of chlorophyll fluorescence induction in the symptomless plants of the cultivars 'Medovyiy' and 'Smena' was from 0.75 and 0.73 and it decreased to 0.63 and 0.69 in plants with FMD symptoms. Structural features of leaf tissue in microshoots of some common fig cultivars grown in vitro were studied for preliminary evaluation of adaptive capacityin ex vitro regenerated plants. Among four investigated cultivars the highest palisade index was noticed for the cultivars 'Pomoriyskiy' (0.44%) and 'Medovyiy' (0.40%). Analysis of water fractional composition demonstrated that free water proportion in the total water content was the lowest in the common fig cultivar 'Medovyiy' – 21.26% and the highest in the cultivar 'Figue Jaune' – 32.42%. The researches allowed identifying cultivar 'Medovyiy', as the cultivar with a high potential for plasticity realization under the following acclimatization in vivo due to its small cells of the covering tissue and large number of trichomes and stomata. T3-P52 STUDIES REGARDING THE NEW PEACH ASSORTMENTS IN SOUTH-EASTERN ROMANIA Corina Gavat1, Liana Melania Dumitru1, Alexandru Oprita1, Liliana Miron2 1 Research Station Fruit Growing Constanta, No. 25 Pepinierei Street, Valu lui Traian, 907300 Constanta, Romania 2 Ovidius University Constanta, no.32-34 Unirii Street, 907532 Constanta, Romania Thousands of peach cultivars are grown in the world and the breeders obtain annually new ones. Peaches are appreciated fruit for their nourishing and therapeutically qualities, both in Romaniaand abroad. The peach is a species with good adaptation and high yields under Romanian climatically and soil conditions. The Romanian breeders made effort to create valuable native cultivars. The aims were to obtain new peach, pavie, nectarine and brugnone cultivars with high qualities of fruit; big and constant productivity of trees; different habits of trees; various ripening time, etc. )n the last th years very good results had: Raluca , Cecilia , Filip and Florin -peach cultivars; Catherine sel. and Craita - clingstone varieties; Costin and Marina - nectarine cultivars; Anemona - brugnone variety. 164 III International Symposium on Horticulture in Europe – SHE 2016 T3-P53 STUDY CONCERNING THE MAIN CHARACTERISTICS OF CLIMBING BEAN SEEDS BELONGING V.R.D.S., ROMANIA GERMPLASM COLLECTION Eliza Teodorescu Mesteacanului Street, No 23, 120024 Buzau, Romania. Since at Vegetable Research and Development Station V.R.D.S. Buzʻu was initiated a program in order to preserve and valorize local bean population (Phaseolus vulgaris L.) from East and South – East of Romania. In 2014 the germplasm collection contained over 150 accessions. This paper presents the comparative study of the main characteristics of 40 climbing bean seed (weight, length, width, thickness, color and secondary colour distribution). In what it concerns V6, there were registered the highest values of seeds weight (0,84 g) and width (10,83 mm). Seeds length varied between 18,05 mm (V7) and 10,74 mm (V36). In what it concerns V36, there was registered the lowest seed weight (0,3 g). The highest value concerning the width/thickness proportion was 1,92 (V7). More than half of the studied seeds presented 2 or more colors dispensed around hilum, on half of grain, in streaks or patches. These results dignify a great variability in what it concerns the biological material collected and also justify the works concerning the biodiversity preservation for this species. T3-P54 THE BEHAVIOUR OF LOCAL POPULATIONS OF ASTER NOVAE ANGLIAE L. IN DIFFERENT GROWING CONDITIONS Lucia Draghia1, Mirela Murariu2, Elena Liliana Chelariu2, Maria Brinza2 1Mihail Sadoveanu Alley, 3, 700490, Garabet Ibraileanu, 6, Bl. 7, Sc. A, et. 1, 700490 Iasi, Romania; 2Alley Sadoveanu nr. 3, Iasi, Romania Study regarding Aster novae-angliae plants (Asteraceae family) reaction at irrigation and fertilization was done at the University of Agricultural Sciences and Veterinary Medicine from Iasi, Romania, using three local populations of traditional garden from NE area of Romania, respectively Suceava county (Marginea and Humoreni localities) and Botosani county (Darabani locality). From the combination of two experimental factors from each population resulted four experimental variants: V1 (control variant) – non-fertilized and non-irrigated; V2 - fertilized and non-irrigated variant; V3 - fertilized and irrigated variant; V4 - irrigated and non-fertilized variant. Foliar fertilization with Cropmax® was carried out. Measurements carried out have shown favorable response of plants, in particular for irrigation. Water stress considerably diminished value of decorative plants (branching capacity reduction, decrease of flowers number and of their size, etc.), while irrigation with or without fertilization obviously favored the appearance of plants. The total content of photosynthetic pigments recorded higher values to plants where fertilization and irrigation was applied (V3) or only irrigation (V4). Culture technology influence on chlorophyll content at three populations of Aster novae-angliae is indicated by the increases obtained at V3 and V4 variants. a / b chlorophyll report at the three Aster novae- angliae populations presented the lowest values at V1 and the highest values of the pigments / carotenoids chlorophyll at V3 plants. It was noted, however, differential response of populations to action of the two experimental factors. Although all three populations of A. novae-angliae studied, by irrigation and fertilization some ornamental characteristics of plants have been favored, but the proportions were different. Thus, the results derived from the population of Botoşani indicate a moderate reaction or smaller differences between control and other variants. 165 BOOK OF ABSTRACTS T3-P55 THE BEHAVIOUR OF SOME APRICOTS VARIETIES WITH LATE BLOOMING IN SOUTH-EASTERN ROMANIA Vladut Alexandru Oprita, Corina Gavat Research Station Fruit Growing Constanta, No. 25 Pepinierei Street, Valu lui Traian, 907300 Constanta, Romania; The breeding of apricot tree for different features is concerning of many researchers over the world where the apricot growths. In Romania, the programme for apricot breeding started since 1952. The main objectives were: enlarge the harvest season, high quality of the fruit, good taste, resistance to transport, tolerance/resistance to the main diseases, etc. Also, the creation of new apricot varieties with late bloom and high resistant to the return frost was one of the aim of the breeders. A good starting point for this work was the apricot germplasm found from Research Station for Fruit Growing Constanta (RSFG Constanta) which had more than 600 genitors. Standard breeding techniques as cross pollination, self-pollination and open pollination were used; more than 18000 hybrids have been evaluated and 380 genotypes were tasted. From these apricot varieties were homologated. Two of them Auras and Augustin showed late bloom days after Goldrich variety that was used as control and good average yields . t/ha and 14.0 t/ha respectively for a density of 625 trees/ha). Also, the new apricot varieties mentioned proved to have high fruit weight, flesh firmness, very good aroma, red skin and good commercial aspect. T3-P56 THE BEHAVIOUR OF SOME NEW CHERRY TREE CULTIVARS DURING THE FIRST YEARS AFTER PLANTING Ion Caplan1, Gheorghe Lamureanu2, Constanta Alexe3 1Fruit Research & Development Station, Valul lui Traian, Amurgului Street 8a, Constanta 90083, Romania; 2No. 25 Pepinierei Street, 907300 Constanta Valu lui Traian, Constanta, Romania; 3RIPMHP Bucharest, no. 1A Binelui Alley, 042159 Bucharest, District 4, Romania; Although the pedo-climatic conditions of Dobrogea are more favourable to the cherry tree culture, this species is not as widespread as the peach tree or the apricot tree. In this area there has not been a constant preoccupation for the establishing of a qualitatively adequate assortment. The large plantations which were organised between 1975 and 1995 were not based on thorough studies and the inappropriate placing lead to many plantations being compromised. The carried out research had as purpose the identifying of cultivars with the best capacity of adapting in the area, expressed through: normal evolution of the growth and fructification phenophases, production in accordance to the age and good quality of the fruit. The research also aimed to establish an assortment of cultivars with a ripening period from early to very late. 31 cultivars were studied, both of Romanian origin R)FG Pitești, RSFG )ași, RSFG Bistrița as well as foreign USA, Germany, Sweden, Ukraine, Czech Republic), grafted on the main parent stock recommended in the area – Prunus mahaleb. The research focused on the behaviour of each cultivar during the first years after being planted in an orchard, specifically the growth vigour during year IV of vegetation, the garnishing of the skeletal branches with anticipated shoots and the type of fructiferous formations. The obtained results lead to the conclusion that the cultivars most likely to start fruiting early (number of anticipated shoots per linear metre are: Sam, Severin, Kristin, Cʻtʻlina, Cerna, Scorospelka and Colina. Among the cultivars with low vigour are Amar Galata, Stella, (edelfinger, Cʻtʻlina, while among those with greater vigour are Colina, Altenburger, NΑ , NΑ , New Star, Ponoare and so on. Several cultivars, namely Cʻtʻlina, Van, Scorospelka, Jubileu, Rainier, Sam, Severin and Izverna are of greater interest for production within commercial orchards since they display the most appreciated fructiferous formation for the cherry tree – the bouquet. 166 III International Symposium on Horticulture in Europe – SHE 2016 T3-P57 THE EFFECT OF ARABIDOPSIS P4H3 ON GLYCOPROTEIN EXPRESSION AND THE FERMENTATION PATHWAY IN RESPONSE TO HYPOXIA Panagiotis Kalaitzis, Zeina El-Zein, Theodore Spano, Klepadlo Mariola Department of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute Of Chania, Makedonias 1, 73100 Chania, Greece. Prolyl 4-hydroxylases catalyze the hydroxylation of proline residues in hydroxyproline rich glycoproteins (HRGPs). HRGPs are broadly implicated in growth and developmental programs as well as responses to abiotic stress such as hypoxia and anoxia. Arabidopsis seedlings are sensitive to low oxygen environments. In this study, reverse genetics was employed to study the biological function of AtP4H3 using a P4H3 T-DNA knock-out mutant (p4h3-2) and over-expression mutant (35S::P4H3) lines. Moreover, the abundance of arabinogalactan and extensin proteins under conditions of oxygen deprivation was investigated using western blot analysis. Ethanolic fermentation was assessed in wild type, P4H3 knock-out (Atp4h3-2) and overexpression (35S::P4H3) mutant lines by measuring ADH and PDC activities in shoots and roots of 7-day old seedlings treated with hypoxia for 1, 2, 4 and 6 hours. The results showed strong expression of arabinogalactan and extension proteins in response to hypoxia in the wild type and P4H3 mutant lines indicating their involvement in the response under conditions of absence of oxygen, probably through their contribution to cell structural and/or functional integrity. In addition, an increase in the ADH and PDC activities in response to hypoxia in roots and discrepancies in enzymatic activities in response to hypoxia were observed in shoots. This study will help demonstrate the importance of proline hydroxylation in plant abiotic stress which could be used as a target mechanism for regulating stress responses in plants T3-P58 THE EXPRESSION OF ETR1 AND CTR1 GENES IN 1-MCP TREATED SPRAY CARNATION Tolga Izgu1, Esra Boz2, Ozhan Simsek2, Murat Ozmen3, Okan Ozkaya2, Omur Dundar2, Jaime A. Teixeira da Silva4, Yesim Yalcin Mendi2 1University of Ege, Faculty of , Agriculture, Department of Horticulture, Plant Biotechnology Lab., 35100 Bornova Izmir, Turkey; 2University of Cukurova, Faculty of , Agriculture, Department of Horticulture, Balcali, 01330 Adana, Turkey; 3Adana Veterinary Control and Research , Institue, Cukurova, 01122 Adana, Turkey; 4Miki-cho Post Office, PO Box 7, Ikenobe , 3011-2 , Kagawa-ken 761-0799, Japan. This study was conducted to determine the effect of 1-methylcyclopropene (1-MCP) on the shelf life and morphological characteristics of two commercial carnation Dianthus caryophyllus L. cultivars, Natila and Amelia . Two different doses of -MCP and nL L− were applied for h at °C after harvesting cut flower stems, which were then stored at °C for days after the application of -MCP. During this storage period, petals were sampled at 12 and 24 h, and 3, 6, 10, 15 and 21 days after treatments began, and morphological plant characteristics were determined and compared. Ethylene production was investigated following a short exposure to 1-MCP and nL L− as was the expression of the ethylene receptor gene (ETR1) and a negative regulator of the ethylene response pathway (CTR1) . Real-Time PCR analysis was carried out to determine differences in expression between CTR1 and ETR1 genes responsible for ethylene biosynthesis during storage. Petal samples remained fresh and there was no ethylene expression in petal samples, even after 21 days of storage. 1-MCP at nL L− was positively correlated between ETR and CTR1 gene synthesis. Moreover, as ETR1 synthesis increased, CTR1 gene synthesis decreased regularly after h of storage in Natalia . (owever, ETR gene synthesis in Amelia was unstable and CTR synthesis decreased within the same time frame. 167 BOOK OF ABSTRACTS T3-P59 THE IDENTIFICATION OF WALNUT CULTIVARS FROM NIKITA BOTANICAL GARDENS BY SSR-MARKERS Sergey Khokhlov1, Ilnur Balapanov2, Ivan Suprun2, Sergey Tokmakov2, 240 1Crimea, Russian Federation, Yalta, Russian Federation; years of Victory street, 39, 350901 Krasnodar, Russian Federation. Walnut (Juglans regia L.) is the crop, which has the high potential for development on the South of Russia. In this study the identification of 15 walnut cultivars from Nikita Botanical Gardens (Yalta) was done by using seven SSR-markers developed for J. nigra. Cultivar Chandler was taken as a standard. The allelic polymorphism data allow to evaluate the genetic diversity of walnut on the territory of the peninsula. The number of alleles for each locus ranged from 6 to 14. All 69 alleles were obtained, in average 10 alleles per locus. The results of done investigations allow to determine the genetic similarity for studied cultivars and the standard. Dendrogram, done during the classification of experimental data, divides the analyzed cultivars into 2 large clusters. That speaks about the heterogeneity of walnut population in the Crimea. T3-P60 THE INFLUENCE OF THE ROOTING SUBSTRATE AND GROWTH REGULATORS IBA AND NAA IN THE NUMBER AND LENGTH OF ADVENTIVE ROOTS OF HARDWOOD CUTTINGS IN BLUEBERRY CV. BLUECROP (VACCINIUM CORYMBOSUM L.) Sabri Braha 1, Petrit Rama 2 1 Janina str. 2 7000 Ferizaj, Janina str. 2 7000 Ferizaj, Janina str. 2 7000 Ferizaj, 7000 Ferizaj, Kosovo 2 Tirane, Tirane, Albania The purpose of this research is to determine the impact of the turf-only substrate and turf–perlite in the ratio 2:1 and of growth regulators in the quality of adventive roots ( the number and length) of well lignified oneyear old branches without fruit buds in the Bluecrop cultivar (Vaccinium corymbosum L.) taken at the end of the latent period before budding at the end of March during the -2015 growing season. In order to support the increase of the number of roots and their length the hardwood cuttings are treated with different IBA and NAA concentrations (1500, 3000, 4500 ppm), while a part of cuttings were untreated control. The number and the length of roots have increased in relation to the increase of concentration from 1500 to 3000 ppm followed by a decline of these values in concentrations over 3000 ppm. Respectively, the number of roots (8) and the higher values of root length (4.6 cm) are achieved in the turf–perlite substrate, IBA 3000 ppm (compared to the turf-only substrate). The presence of perlite helps the aeration of the substrate and supports biochemical and physiological processes which lead to the inducing of adventive roots. Regarding the number and length of roots an important variation for (p<0.05) was observed between different concentrations of IBA and NAA. In general the effect of IBA was a lot better than the effect of NAA. T3-P61 THE NEW WALNUT BREEDING PROGRAM IN TURKEY 1University 2Kahramanmaras Yasar Akca1, Mehmet S“tyemez2 of Gaziosmanpasa, Faculty of Agriculture , Department of Horticulture, Turkey; S“tç“ Imam University, Faculty of Agriculture , Deparment of Horticulture, Kahramanmaras, Turkey. The most important problem of walnut production in Turkey is variety. Turkish walnut cultivars are considerably early leafing and they are bear fruit on terminal buds. The most widely used breeding method in walnut breeding programs in Turkey is by selection. A cross breeding method was used in only two studies. The new walnut breeding program was started in 2008. The aim of this breeding program is to provide new walnut cultivars by crossing for the Turkey walnut industry. The primary goal is to develop cultivars with late leafing, lateral fruit bearing, early harvest dates, and good nut quality. Thirteen crosses 168 III International Symposium on Horticulture in Europe – SHE 2016 have been carried out in different three ecological conditions. The varieties with late leafing and good nut quality chosen as female parents, were pollinated by varieties with lateral fruit bearing and high yielding. To ensure the highest possible germination, seed are straficated in cold room (4-5 C). After strafication, germinated seed are planted in large tubes in containing torf, perlit and soil for growth in the greenhouse. We have 1340 seedlings under evaluation in the breeding program. Leafing time of seedlings was observed in greenhouse at second year; and late leafing seedling were selected and planted in a breeding plot. Leafing time of seedlings are determined and compared by Franquette, Serr and Chandler cv during three years in breeding plot. If no nuts have been produced by age four they are painted by red. The results showed that 189 genotypes were selected according to late leafing, and six genotypes early fruiting and late leafing. In 1340 hybrids observed, 35.6 % of seedling varied from medium late to very late leafing. The data showed that there are correlation between the leafing time of one year and three year. Six early yielding genotypes and 15 genotypes with late-very late leafing according to Franquette cv.and early falling of the leaves according to Chandler were grafted. The sensitivity of the promising genotypes resistant to bacterial blight will be determined. Nut weight and kernel weight of selected F1 genotypes varied from 34,43 g to 15,00 g and 14,95 g to 8,00 g respectively. Selected walnut F1 genotypes are tolerant to bacterial blight. T3-P62 THE PEACH HECATE3-LIKE GENE FLESHY PLAYS A DOUBLE ROLE DURING FRUIT DEVELOPMENT Alessandro Botton 1, Angela Rasori , Fiorenza Βiliotto , Annick Moing , Mickaël Maucourt , Stéphane Bernillon , Catherine Deborde , Anna Petterle 2, Serena Varotto 2, Claudio Bonghi 2 1 University of Padova - DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly 2 DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly 3 INRA, UMR1332 Biologie du Fruit et Pathologie, 71 av Edouard Bourlaux, 33140 Villenave dOrnon, France 4 )NRA, Metabo(UB, )BVM, Plateforme Métabolome du Centre de Génomiqu, av Edouard Bourlaux, 33140 Villenave dOrnon, France Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model able to depict the regulatory networks governing this relevant part of fruit development is still missing. Here we described the peach fruit as a system to link the phenotype of a slow ripening (SR) selection to an altered transcriptional regulation of genes involved in determination of mesocarp cell identity providing insight toward molecular regulation of fruit tissue formation. Morpho-anatomical observations and metabolomics analyses performed during fruit development on the reference cultivar Fantasia, compared to SR, revealed that the mesocarp of SR maintained typical immaturity traits (e.g. small cell size, high amino acid contents and reduced sucrose) throughout development, along with a strong alteration of phenylpropanoid contents, resulting in accumulation of phenylalanine and lignin. These findings suggest that the SR mesocarp is phenotypically similar to a lignifying endocarp. To test this hypothesis, the expression of genes putatively involved in determination of drupe tissues identity was assessed. Among these, the peach HEC3-likegene FLESHY showed a strongly altered expression profile consistent with pit hardening and fruit ripening, generated at a post-transcriptional level. A double function for FLESHY in channelling the phenylpropanoid pathway to either lignin or flavour/aroma is suggested, along with its possible role in triggering auxinethylene cross talk at the start of ripening. 169 BOOK OF ABSTRACTS T3-P63 THE RESISTANCE OF APRICOT GENOTYPES TO THE ATTACK OF CERTAIN PATHOGENS IN ROMANIA Cristina Moale 1, Leinar Septar 2 1 Research Station Fruit Growing Constanta, No. 25 Pepinierei Street, Valu lui Traian, 907300 Constanta, Romania 2 25 Pepinierei, Valu lui Traian, 907300 Constanta Constanta, Romania The apricot tree is among the most valuable fruit-growing species due to its biological characteristics such as: the quick beginning of the ripening stage, a large and constant production every year, the ecological plasticity and the special quality of the fruit. Unfortunately, however, the optimum growth conditions also favor the attack of certain pathogens which cause very damaging diseases. Among the pathogens which have an economic importance for the apricot tree culture, because of the fact that they are involved in the premature demise of this fruit-growing species are the following fungi: Monilinia laxa, Cytospora cincta and Stigmina carpophila. These pathogen agents cause severe diseases that affect not only the fruit production but the life of the trees itself, taking into account the climatic conditions of a specific year and especially the soil s sensitivity. The aim of this research is to highlight the genotypes that are the most resistant to pathogens and their behavior towards the pathogens attack in the pedo-climatic conditions of Romania. With regard to the attack of the Monilinia laxa fungus, the majority of the studied cultivars proved to be tolerant T . The Orizont , Danubiu , Cristal and Olimp cultivars displayed sensitivity S towards Monilinia laxa. In this pathogen s case, we must mention the fact that the sensitivity of the cultivar is also influenced by the environmental conditions of each studied year. The cultivars that are very resistant, basically not attacked by Monilinia laxa and Cytosphora cincta are Traian , Tudor , Elmar , Ovidius and Amiral , whereas the most sensitive cultivars to the attack of Cytospora cincta and Stigmina carpophila are Augustin , (istria , Cristal and Euxin . As a result of this study, the most valuable genotypes are to be cultivated in commercial plantations, occupying a good place in the structure of the apricot tree assortment from the south-eastern part of Romania. T3-P64 USAGE OF SEWAGE SLUDGE IN BRASSICA OLERACEA ACEPHALA CULTIVATION Özg“r Kahraman, Fatih Akin Canakkale Onsekiz Mart University, Faculty of Architecture and Design, Department of Landscape Architecture, 17020 Canakkale, Turkey; The amount of sewage sludge has increased expeditiously depending on industrial development and population growth. Reuse of the sewage sludge without damaging the nature and the environment is too important in terms of saving the nature. This study was investigated to determine the effects of different sewage sludge ratio (100% sewage sludge + 0% soil, 75% sewage sludge + 25% soil, 50% sewage sludge + 50% soil, 25% sewage sludge + 75% soil, 0% sewage sludge + 100% soil) on development of Brassica oleracea acephala used as ornamental plant in landscape studies in winter growing period between 2015 and 2016. The study was set up using randomized plot design with three replications (each replication had four plants at the open areas of Çanakkale Onsekiz Mart University, Faculty of Architecture and Design. Parameters like plant height, plant diameter, upper part of plant weight, leaf number, root length, root weight, stem diameter and chlorophyll content were analysed by analysis of variance, multiple comparison test and correlation. As a result of this analysis, different sewage sludge ratios have significant effects on upper part of plant weight, plant height, plant diameter, leaf number, root length, and chlorophyll content. The best plant height (13.83 cm) and plant diameter (18.083 cm) were obtained from 100% sewage sludge + 0% soil mixture. 170 III International Symposium on Horticulture in Europe – SHE 2016 T3-P65 VALUABLE BLACKBERRY CULTIVARS TESTED IN RUBUS COLLECTION AT THE RESEARCH INSTITUTE OF HORTICULTURE IN SKIERNIEWICE, POLAND Miroslaw Sitarek, Justyna Wójcik-Seliga Research Institute of Horticulture, Konstytucji 3 Maja 1.3, 96-100 Skierniewice, Poland The blackberry world production is steadily increasing. In 2015 the total area of this crop was estimated to be 30 thousand hectares. In Europe the largest blackberry producers are Serbia and Hungary. There is very difficult to assess how much blackberry fruits produce Poland, because no separate official data in this matter are available, and blackberry is presented together with other fruits with low economic meaning. However, market observations show that in each year blackberry production is increasing. The climate of Poland, due to low temperatures during winters and springs, is not favourable for blackberry growing. For example, in January of 2016 the lowest temperature in the location of blackberry experiment was -18oC. In such conditions most of blackberry plants lost their flower buds and when the temperature drops even more the whole plantation can be ruined. Therefore, the main problem for Polish fruit growers is short assortment of hardy annual cultivars giving a regular yield of large, firm and attractive fruits. In the Research Institute of Horticulture in Skierniewice, central Poland, there is situated Rubus field collection. The collection counts 143 genotypes of local and foreign genesis (cultivars and clones of raspberry and blackberry, wild types of Rubus genus and berry hybrids). Apart from preservation of gene bank resources, there are collected different data on yield and fruit quality of planted cultivars, their resistance to diseases and pests, bush vigour and survival. The results revealed that among cultivars tested Chester , Čačanska Bestrna , Loch Ness and Loch Tay are valuable in Polish climatic conditions and can be cultivated in open area, while Oregon , Black Satin , Black Butte and Karaka Black require cover systems like plastic tunnel or greenhouse. Acknowledgement: This work was performed in the frame of multiannual programme on preservation of gene bank resources financed by the Polish Ministry of Agriculture and Rural Development: Task 1.3 Collecting, preservation in ex situ collections, cryoconservation, evaluation, documentation and using of gene bank resources of horticultural crops . T3-P66 VARIABILITY ON MORPHOLOGIC DESCRIPTORS OF PORTUGUESE M. PULEGIUM ACCESSIONS Orlanda Viamonte Póvoa1, Noémia Farinha1, Leandra Rodrigues2,Ana Maria Monteiro3 Escola Superior Agr|ria de Elvas, )nstituto Politécnico de Portalegre, Quartel do Trem Auto- AP 254, 7350 Elvas, Portugal; 2CEBAL-Centro de Biotecnologia Agrícola e, Agro-alimentar do Alentejo, R.Pedro Soares, Campus IPBeja ESAB Ap. 6158, 7801-908 Beja Beja, Portugal; 3)nstituto Superior de Agronomia, Dep. Ciências e Engenharia de Biossistemas, Proteção de Plantas-Tapada da Ajuda, 1349-017 Lisbo Lisboa, Portugal. 1 M. pulegium is traditionally used in Alentejo to flavour fish and «açordas» recipes, as well as for its medicinal properties. The essential oil has also industrial interest, namely for food preservation and soaps. This study was based on 23 accessions from backyards and natural habitat collected during 2002-2003 in the Alentejo region. From each sample, 15 plants were observed during 2 years growing seasons. Data from 33 morphological descriptors were submitted to multivariate Principal Components Analysis (CPA) and cluster analysis. High morphologic variability in the species was confirmed by its high statistical difference between accessions on most of the morphologic descriptors. The initial list of 33 morphologic descriptors was reduced to a proposal list of 14 descriptors, measuring plant architecture features, productivity indicators and also phenology descriptors: Plant height; Biomass; 171 BOOK OF ABSTRACTS Basal leaf length (cm); Basal leaf width (cm); Inflorescence 1st leaf length (cm); Inflorescence 1st leaf width (cm); Number of days for flowering (date); Main stem length (cm); Main stem diameter – basal; Main stem diameter – below inflorescence; Main stem - number of nodes below inflorescence; Main stem - number of branches below inflorescence; Number of flowers in half of the 1st verticillaster; Number of flowered verticillasters; Seed germination (%). A list of selected accessions with high crop production potential is also presented. T3-P67 WATER DYNAMICS AND PHENOLOGY OF 'EVA' APPLE TREES: COMPARISON OF DIFFERENT PLANT PORTIONS UNDER MILD WINTER CONDITIONS IN SOUTHERN BRAZIL Flavio Gilberto Herter 1, Paulo Mello-Farias2, Caroline Farias Barreto2, Everton Sozo de Abreu2, Henrique Bisognin Gallina2, Izabel Camacho Nardello2, Laura Reisdörfer Sommer2, Marines Batalha Moreno2, Roseli Mello Farias2, Robson Ryu Yamamoto2, Ana L’cia Soares Chaves2, 2Federal 1UFPEL-FAEM, C. Postal 354, 96010-610 Pelotas, Brazil; University of Pelotas, Faculty of Agronomy Eliseu Maciel, Pelotas-RS, Brazil; Apple tree during the period of low temperatures goes through a dormant phase. Water plays an important role in plant metabolism and may be responsible for breaking dormancy. Phenological behaviour and water dynamics studies of apple cultivars are very important to understand adaptation under adverse climatic conditions. This research aimed to evaluate the phenology and water dynamic of different portions of Eva apple trees to elucidate bud breaking under mild winter conditions at Pelotas area, RS, Brazil. Evaluations were conducted in the orchard at Federal University of Pelotas during winter season of , with Eva apple trees grafted on Marubakaido rootstock and intergrafted on M-9, implanted in 2012. Samples of plant material were collected four times from these plants, it was removed 3 branches per plant of each portion (basal, middle and apical) of the canopy. These branches were separated in three parts (bud, bark and wood) and weighed on analytical balance to measure the fresh weight and then dried to measure the dry weight. Water content was determined by the formula CA = (MF-MS) / MS. Phenological evaluations based on stages 'C' (green tips), 'D' (half-inch green leafless) and 'F' (beginning of flowering) were used as reference. The treatments were basal, median and apical plant portions in a completely randomized design with five replications and eight evaluation dates. Results showed no significate difference among the different parts of the plant and the branch, and CA was always higher in plant bark tissue. Results showed that apical part presented higher percentages of bud burst, while the basal part had the lowest percentages and followed by an acropetal standard of sprouting, demonstrating a high cultivar adaptation to low-chill winter conditions. This study concludes that 'Eva' cultivar has great potential for cultivation in mild winter areas. T3-P68 WATER USE EFFICIENCY OF HYBRID VS OPEN POLLINATED TOMATO (LYCOPERSICON ESCULENTUM) GENOTYPES Rabab Sanoubar, Giuseppina Pennisi, Francesco Orsini, Fiorenzo Salvatorelli, Antonio Cellini, Francesca Ventura, Francesco Spinelli, Enrico Noli, Sonia Blasioli, Ilaria Braschi, Giorgio Prosdocimi Gianquinto Dip. Scienze e Tecnologie Agroambientali, DiPSA, Universit{ degli Studi di Bologna, Viale Fanin, - 40127 Bologna , Italy Water availability is considered as one of the most determinant cultivation factors and seriously affects plant growth in several world agricultural regions. The identification of water efficient crop species and cultivars is currently one of the main objectives of research in plant sciences. Tomato (Lycopersicon esculentum) is one 172 III International Symposium on Horticulture in Europe – SHE 2016 of the mostly grown horticultural crops with reported irrigation water use efficiency in the Mediterranean basin of 20 to 80 kg m-3. In the past decades, commercial plant breeding has mainly addressed yield or quality traits, with little effort spent on improving drought tolerance. The identification of drought tolerant lines was mainly addressed by breeding water efficient rootstocks, which often are selected among wild tomato relatives. In the present work, 29 tomato genotypes (14 hybrids and 15 open pollinated lines) are compared for their physiological and biochemical response to drought (by reducing water potential of the irrigation water down to 0, -0.5 and -1 MPa through PEG application) in a growth chamber experiment. Among the studied lines, 14 genotypes (7 hybrids and 7 open pollinated lines) were selected based on their specific water relation traits (e.g. stomatal conductance, RWC, overnight water loss) and used in a field lysimeter experiment in which deficit irrigation treatments (respectively providing 100%, 50% and 25% ET estimated by Hargraves equation) were applied. Among studied genotypes, water use efficiency features are in the present research related with differences in accumulation of biochemical compounds. T3-P69 EFFECT OF VARIOUS CULTURE MEDIA ON IN VITRO PROPAGATION OF GRAPEVINE VARIETIES GIOUROUKIKO AND SERIFIOTIKO (VITIS VINIFERA L.) Katerina Biniari,Styliani Kypraiou, Maritina Stavrakaki, Despoina Bouza Agricultural University of Athens, Iera Odos 75, Athens, Greece The objective of this work was to evaluate the effect of different culture media and the influence of the position of nodal segments on blastogenesis and rhizogenesis, on the in vitro propagation of grapevine varieties Giouroukiko and Serifiotiko that are preserved in the Ampelographic Collection of the Agricultural University of Athens. For the in vitro propagation, the culture media MS (Murashige & Skoog), MS (Murashige & Skoog) modified and WPM (Woody Plant Medium), full or half strength were used. The explants, one-node-cuttings, came from the base, the middle and the tip part of the main shoots. The results showed that: a) the position of nodal segments affect the blastogenesis, while the variety also plays a significant role. In grapevine variety Giouroukiko, the explants of the base part of the shoot showed the highest percentage of blastogenesis, while in the case of grapevine variety Serifiotiko, it was the explants of the middle part of the shoot, b) regarding the formation of adventitious roots no statistically significant differences between the three categories of explants were observed, although the average number of roots per explant was higher in the explants of the base part of the shoot in both varieties, c) regarding the six different culture media used, WPM 100% gave the best results for variety Giouroukiko while MS modified 50% for variety Serifiotiko, and d) regarding the rhizogenesis induction, no statistically significant difference was observed between the two varieties and the culture media used. 173 BOOK OF ABSTRACTS Poster Topic – 4 Tools, Technologies, Innovations and Applications 174 III International Symposium on Horticulture in Europe – SHE 2016 T4-P1 A DEMONSTRATIVE MODULE TO PROCESS CHILI PEPPER PRODUCTS BY FAMILY FARMERS IN CHILE Diego Munoz-Concha, Ximena Quinones, Juan Pablo Hernandez, Ricardo Castro1, Nelson Loyola Universidad Catolica del Maule, Carmen 684, Curico, Region del Maule, 3341695 Curico, Chile; The Region of Maule is one of the poorest areas of Chile and has the highest proportion of rural inhabitants of the country. Most local growers devoted to the production of chili pepper (Capsicum annuum), sell their products as unprocessed dry or grinded fruits, while industries and processors often process their products in a different region of the country. Occasionally local family farmers produce small amounts of processed products, which are sold in informal markets as homemade items. In such cases, however, small producers do not comply with the Chilean food sanitary regulations, which prevents them from entering their processed products into formal markets. They, therefore, miss the opportunity to obtain an added value for their products. As part of a project funded by a regional government department, a demonstrative module has been planned and built to allow local producers to get sanitary permissions to process and sell their chili pepper products. This module can be relocated in order to visit the producers of the two main geographical areas of chili pepper production in the region. Its design includes areas for washing, preparing and packing, as well as the necessary processing equipment. The information and transference of technology that this module brings to small growers will allow them to meet the requirements for sanitary permissions, enabling local chili pepper products to enter formal markets, as well as the potential inclusion of other local vegetablederived products. T4-P2 A NEW STRATEGY TO STUDY INTRA-FRUIT RIPENING PROGRESSION IN APPLE (MALUS DOMESTICA L. BORKH) Francesca Populin1, Giulia Eccher1, Mirko Moser2,Sergi Munne-Bosch3, Javier Miret3, Annick Moing4, Mickaël Maucourt5, Sylvain Guyot6, Agnes Gacel6, Duyen Prodhomme4, Elisa Asquini2, Azeddine Si-Ammour2, Alessandro Botton1 of Padova - DAFNAE, Agripolis, Viale dell'universit{, , Legnaro, )taly; 2FEM, Via E. Mach, 1, 38010 San Michele all'Adige(Trento), Italy; 3Dept. of Plant Biology, Universitat de Barcelona, Barcelona, Spain; 4INRA, UMR1332 Biologie du Fruit et Pathologie, 71 av Edouard Bourlaux, 33140 Villenave dOrnon, France; 5INRA, MetaboHUB, IBVM, 71 av Edouard Bourlaux, 33140 Villenave dOrnon, France; 6Chargé de Recherches, Equipe PRP, Domaine de la Motte BP 35327, 35653 Le Rheu, France. 1University Apple (Malus domestica L. Borkh) fruit ripening is the last important and well characterized step of fruit development in which apple reaches its full size and acquires the organoleptic characteristics to become edible. This process is strictly regulated by both exogenous (i.e. light and temperature) and endogenous (i.e. hormones) factors. Despite the huge amount of data available about the ripening syndrome in apple, only few information exists concerning the real starter of this process. Moreover, no information is available about how the ripening syndrome progresses through the fruit. For this reason a new protocol was set up to study the ripening progression in fruits of the cv. Golden delicious. Samples were collected from 90 days after full bloom (DAFB) up to 180 DAFB. Transcriptional, metabolomic and hormonal profiling analyses were performed and results integrated through a dedicated bioinformatic pipeline. Unexpected internal gradients in terms of transcripts, metabolites, starch, and hormones were observed, suggesting that the maturationripening syndrome proceeds according to an internal gradients following a starter signal that is next to be identified in experiments that are currently being carried out. 175 BOOK OF ABSTRACTS T4-P3 AGRO-INDUSTRY COMPOSTS AS GROWING MEDIUM IN A FLOATING SYSTEM FOR GROWING BABY-LEAF LETTUCES. ADDED-VALUE TO SUPPRESS PYTHIUM IRREGULARE Juan A. Fernandez1, Almudena Giménez1, Catalina Egea2, Ana B. Santísima-Trinidad3, Margarita Ros3, Jose A. Pascual3 1Dpto. Producción Vegetal, Univ. Politécnica de Cartagena, Paseo Alfonso X))), , Cartagena Murcia , Spain; Ciencia y Tecnología Agraria, Universidad Politécnica de Cartagena, Paseo Alfonso X))), , Murcia Cartagena, Spain; 3CEBAS-CSIC , Campus de Espinardo, 30100 Murcia Murcia, Spain; 2Dpto. Production of small leafy vegetables (baby leaf) in floating system can be affected by different diseases such as damping off , which is caused by fungi as Pythium spp. Compost management can be used as added-value organic growing media as alternative to peat that it is the traditional one in floating system. The main issue to obtain added-value compost is by selecting appropriate and specific type and rate of feedstock or by microbial inoculation. The objective of this work was to investigate the suppressive capacity of three agroindustry compost (C9, C11 and C14) in the pathosystem Pythium irregulare-lettuce. The three compost were managed attending the initial feedstock and/or microbial inoculation. Compost C9 was composed mainly by pepper sewage sludge, pepper peels, carrot and vineyard pruning material, compost C11 showed similar feedstock inoculated with Aspergillus niger and compost C14 was mainly composed by tomato and onion peels plus vineyard pruning material. Two different ratio compost/peat (70/30 and 100/0 v/v) were used to fill the trays and the percentage of seed germination and compost suppressiveness was determined at 8 days and 20 days after sowing, respectively. The highest percentage of germination and compost suppresiveness was obtained in the compost C14 for both compost/peat ratios assayed, showing higher fresh and dry weight and less pathogen affection. The treatments that only incorporate compost showed higher fresh and dry plant weight, no showing any symptom of toxicity in plants. P. irregulare was measured by Real-time PCR in the growing media and in the roots. Compost C14 also showed the lowest pathogen amount compared to the rest of treatments but not in roots, which showed similar values. This result makes us to go into detail about the potential induced resistance of compost C14 into plant to avoid that pathogen infection affects lettuce growth. In this case, it was relatively important the initial composition of compost, that permits to obtain a suppressive added-value compost. T4-P4 BENEFICIAL ROLE OF BIOFERTILIZATION ON YIELD RELATED CHARACTERISTICS OF TWO APPLE CULTIVARS AND SOIL MICROORGANISMS UNDER ORCHARD CONDITIONS Marijana Pesakovic 1, Milan Lukic 2, Jelena Tomic 2, Zaklina Karaklajic-Stajic 2, Rade Miletic 2, 1 Fruit Research Institute, 32000 Cacak, Serbia 2 Kralja Petra I 9, 32000 +38132221413 Cacak, Serbia Intensive development of agriculture and increasing utilization of synthetic nitrogen fertilizers significantly contributes to a series of undesirable effects and results in excessive environmental pollution. Approximately half of all nitrogen applied to boost agricultural production escapes its intended use and is lost to the environment. Over the past years, fruit growing practice has been focused on new concept which relies primarily on the application of microbial inoculants i.e. biofertilizers for gaining certain physical, chemical and microbiological properties of soil, as well as plant growth promotion. In line with the concept, we conducted comparative study of the effect of bio- and chemical fertilizer applications on yield-related characteristics of Granny Smith and Čadel apple cultivars and microbiological properties of the soil. The yield-related characteristics of the tested plants were evaluated for generative potential parameters i.e flowering intensity, fruit set intensity, final fruit set intensity and yield (kg tree-1; t ha-1).The microbiological properties of soil were monitored by determining the total microbial count, numbers of soil fungi, 176 III International Symposium on Horticulture in Europe – SHE 2016 actinomycetes, oligonitrophilic bacteria and azotobacter. The analysis of the results points to the fact that the efficient apple nutrition management should ensure both enhanced and sustainable production. This approach seems to contain a certain potential as an appropriate technique in commercial apple production, which may improve yield-attributing characteristics and soil fertility. T4-P5 BIOFORTIFICATION OF OCIMUM BASILICUM L. PLANTS WITH SELENIUM Martina Puccinelli1, Fernando Malorgio1, Rita Maggini1, Irene Rosellini2, Beatrice Pezzarossa2 1 Department of Agriculture, Food and Environ, University of Pisa, Viale delle Piagge 23, 56124 Pisa, Italy 2 CNR, Institute for Ecosystem Study , Pisa, Italy Sweet basil (Ocimum basilicum L.) is an aromatic annual plant belonging to the Lamiaceae family, cultivated and utilized throughout the world. Basil has a culinary value but also pharmaceutical properties. The aromatic oil obtained from its leaves has antioxidant and antimicrobial activity. Selenium (Se) is an essential element for humans, and acts as antioxidant and anticarcinogenic. Since healthful nutrient plant compounds are more bioavailable than compounds contained in food supplements, biofortification of fruiting and leafy vegetables with Se is a good way to increase consumption and supplementation of Se by humans. Further, Se appears to be effective in delaying fruit ripening and plant senescence, thus decreasing postharvest losses due to enhanced antioxidation. We investigated the effect of Se addition on production and quality traits of Ocimum basilicum L. cv Tigullio . Two experiments were conducted on sweet basil seedlings grown in greenhouse in hydroponics at rate of 0 (control), 0.5, 1, 2, 4, 8 and 12 mg Se L-1. Selenium was added to the nutrient solution as sodium selenate. In order to evaluate the growth, physiology, and nutritional value in basil the following parameters were determined at harvest time: plant biomass, antioxidant capacity, total phenols, rosmarinic acid, nitrate, macro- and micronutrients, total chlorophyll and total carotenoid. Our results showed that the addition of selenium significantly and linearly increased the Se content in basil. In general the Se did not affect the plant biomass and the quality of product. The amount of Se accumulated in plants could provide the rational Se intake for human nutrition in accordance with the recommended dietary allowance (RDA) guidelines. T4-P6 CHANGES IN SURFACE FEATURES OF POMEGRANATE FRUIT WITH ON-INVASIVE SENSOR TECHNOLOGY Michael Blanke 1, Lutz Damerow 2 1 Institut Obstbau Bonn, Auf dem Hugel 6, 53121 Bonn, Germany 2 Nussallee 5, 531115 Bonn, Germany Non-destructive methods for determining quality of fresh horticultural produce are needed to help meet market demands and enhance overall sustainability of production. Two innovative methods were tested for this purpose: a new luster sensor technology (type CH-72)and three-dimensional microscopy (type VHX 5000)as well as the combination of both innovative techniques. Αellow cv. Golden Globe and red cv. Wonderful pomegranate fruit were stored over three weeks either at room temperature at °C VPD . mbar or in the fridge at °C VPD . mbar both at % rh. Changes in micromorphology, i.e. degree of fruit shrivelling and the roughness as depth of shrivel or undulations were examined. The luster levels decreased during the time of the experiment. The luster level of red pomegranate fruit dropped from 194-340 (averaged 250) relative units to 195- 270 (averaged 220) relative units after three 177 BOOK OF ABSTRACTS weeks of storage at °C room temperature. The luster levels of yellow fruit dropped more dramatically from 200-410 (averaged 310) relative units to 175-210 (averaged 190) relative units after storage at room temperature. These changes in luster level were associated with a c. 2.5-3 fold increase in undulation; the average depth of fruit surface undulation increased from 0.45 mm with fresh to 1.4 mm in fruit stored at room temperature. Luster levels and 3D images of pomegranate fruit were recorded for the first time, the results may indicate that the luster sensor may be used to detect changes in surface appearance like glossiness and degree of shrivelling in yellow, but to a lesser extent in red pomegranate fruit; further research is required before a commercial application. T4-P7 CHARACTERIZATION OF BLUEBERRY AROMA BY NOVEL FAST-GC COUPLED WITH PTR-TOF-MS Brian Farneti, Iuliia Khomenko, Luca Cappellin, Marcella Grisenti, Paula Poncetta, Paolo Loretti, Matteo Ajelli, Franco Biasioli, Lara Giongo Via Mach 1, 38010, San Michele all'Adige (TN), Italy; The availability of rapid and accurate methods to assess fruit flavour is of utmost importance to support quality control especially in the breeding process. Breeders need more informative and analytical tools to facilitate the selection of complex multigenic traits such as flavour quality. In this study, the volatiles emitted by 13 Vaccinium accessions (at full ripe stage) were analysed by two solvent-free headspace methods: Proton Transfer Reaction - Time-of-Flight - Mass Spectrometry (PTR-ToFMS) direct injection and PTR-ToF-MS coupled to a novel Fast-GC system that allows a rapid (120 s) chromatographic separation of the sample. The combination of these two analytic strategies permits the contemporaneous unsupervised (direct injection) and supervised (FastGC) analysis of aroma compounds of blueberry in few minutes. Multivariate statistics (principal component analysis and cluster analysis) of the results allow an unambiguous separation between genotypes based on aroma fingerprinting. These results show that PTR-ToF-MS coupled to a Fast-GC is suited to monitor at high sensitivity the emission of a large number of volatiles that describe the blueberry aroma profile. This technology can easily monitor and quantify compounds related to ripening and/or senescence so that it can be used to improve the breeding based on volatiles. T4-P8 CHARACTERIZATION OF NITROGEN AND CARBON POOLS OF ORGANIC FERTILIZERS TO PREDICT THEIR NITROGEN RELEASE Elke Meinken, Jakob Burmann1, Dieter Lohr Am Staudengarten 14, 85354 Freising, Germany During the last years customer demand for organically grown potted plants – not only herbs but also ornamentals – sharply increased. Due to fast growth and high optical requirements nitrogen fertilization is one of the greatest challenges for growers. Beside total amount of released nitrogen time course of mineralization is a crucial factor for the selection of the most suitable fertilizer. However, apart from elaborate incubation experiments there is a lack of methods for characterizing nitrogen release behaviour of organic fertilizers. Aim of the current research was the determination of various nitrogen and carbon fractions of organic fertilizers with different approaches as basis of a prediction model of nitrogen release. In total 14 different organic fertilizers ranging from raw plant material as coarse meal of legumes, to products of animal origin as hoof and horn meal or pig bristles, to residues of industrial processes (e.g. from starch and sugar industries) were included. Nitrogen was analysed photometrically after hydrolysis with cold and hot water as well as with 0.005 M and 1 M HCl respectively. Organic carbon was measured after hydrolysis with 0.005 M and 1 M HCl by ICP-OES. Additionally, organic carbon was fractionated by stepwise 178 III International Symposium on Horticulture in Europe – SHE 2016 pyrolytic combustion between °C and °C. Nitrogen release behaviour was determined in a six-week incubation experiment, analysing mineralized nitrogen at eight different dates. The incubation experiment revealed major differences in time course of nitrogen mineralization. While most of the nitrogen of some fertilizers was available promptly others nearly released no nitrogen within the first two weeks. However, analysed nitrogen and carbon fractions were not suitable to predict time course of nitrogen mineralization. Nevertheless cold and hot water soluble organic nitrogen could be used to estimate the total amount of nitrogen released within six weeks. T4-P9 COMMISSIONING AND OPTIMIZATION OF AN INNOVATIVE SYSTEM FOR ELECTROLYTIC WATER DISINFECTION IN GREENHOUSES Janine Berberich1, Ingo Schuch2, Dennis Dannehl2, Yuan Gao3, Uwe Schmidt2 1 HU Berlin, FG Biosystemtechnik, Albrecht-Thaer-Weg 1, 14195 Berlin, Germany 2 HU Berlin, FG Biosystemtechnik, Albrecht-Thaer-Weg 3, 14195 Berlin, Germany 3 newtec Umwelttechnik GmbH, Am Borsigturm 62, 13507 Berlin, Germany Tested in experimental scale, an innovative system for electrolytic water disinfection in greenhouses (SeWiG) was very efficient. It was developed by Humboldt-Universität zu Berlin and newtec Umwelttechnik GmbH. With scaling up this system for industrial greenhouses, the new technology will be tested, validated and optimized under practical conditions. The implemented technology will be incrementally scaled-up. After successfull installation of the disinfection system within a separated part of an industrial greenhouse, two large-scaled greenhouses will use the on-site produced hypochlorite as a disinfectant for irrigation or drain water applied for vegetables and ornamental plants. The effects on plant growth, yield and product quality will be controlled and particular attention is paid to chlorate and perchlorate. Due to the first results, both pesticides are under the limits given by the European Commission. This can be related to the short dwell time of the disinfectant before dosing based on the on-demand production of fresh hypochlorite. Hypochlorite is dosed by the method of shock disinfection, which might decrease the accumulation of unwanted ingredients in plants. The special feature of the new system for electrolytic water disinfection is the functional superiority over common methods such as filtration, ultraviolet irradiation, heating, ozonation or chlorine dioxide. Worth highlighting is its high effectiveness against plant viruses and the reduced risk for users, plants and the environment while less energy is needed, compared to other disinfection syste Besides a reduced formation of algae biofilm, a reduced application of fertilizers is expected. All parameters mentioned before will be monitored within this study. T4-P10 COMPARISON OF STRAWBERRY (F.XANANSSA CV. FLORIDA FORTUNA) VOLATILES USING VARIOUS SPME FIBRES BY GC/MS TECHNIQUES Nesibe Ebru Kafkas1, mozghan ZARIFIKHOSROSHAHI2, saaed Sadgh Azadi3, hatice özcan3 1Department of Horticulture, Faculty of Agriculture, TR-01330 Adana Balcali, Turkey; 2University of Cukurova, Adana, Turkey; 3University of Cukurova, Faculty of Agricult, TR-01330 Adana, Turkey; The cultivated strawberry Fragaria × ananassa Duch. is an important beery crop in the world due to the having flavourful taste, unique and highly desirable aroma. Sugars, acids, and aroma volatiles contribute to the characteristic strawberry flavour, which is dependent on the proper balance of these chemical constituents. While sugars and acids are responsible for the sweetness and tartness of the fruit, aroma volatiles provide the unique, fruity flavours that characterize a strawberry. Esters are quantitatively and qualitatively the most abundant class among the flavour compounds. Headspace extraction methods provides especially the SPME (Solid Phase Micro Extraction, is a solvent-free, inexpensive, rapid and versatile method for the extraction of flavour compounds. There are many kinds of fibres that used identification of 179 BOOK OF ABSTRACTS volatile compounds. In this paper, it was aimed compare various SPME fibres (CAR/PDMS, PDMS, CAR/PDMS/DVB) using GC/MS (Gas Chromatography Mass Spectrometry) to identify volatile compositions in fruits of Florida Fortuna cultivar. T4-P11 DETECTION OF SALINITY STRESS IN GREENHOUSE TOMATO BASED ON CROP REFLECTANCE Nikolaos Katsoulas 1, Ageliki Elvanidi 1, Eleni Zagari 1, Thomas Bartzanas 2, Panagiotis Kalaitzis 3 , Constantinos Kittas 1, 1 University of Thessaly, Dept. of Agriculture Crop Prod & Rur. Env., Fytokou St, 38446, New Ionia, Magnisia, Greece 2 Centre for Research and Technology Hellas, Dimitriados 95, 38333 Volos, Greece 3 Department of Horticultural Genetics Biot, Mediterranean Agronomic Institute of Chania, 73100 Chania, Greece Salt stress is the most wide spread abiotic stress that limits plant growth, physiology and productivity mainly affecting the ionic balance and plant water or mineral-nutrient relations. When a plant becomes salinity stressed, stress is expressed in many types of symptoms. For example, it decreases leaf chlorophyll content and impedes photosynthesis, resulting in changes in leaf colour. Leaf water potential, leaf gas exchange, leaf pigments and dry matter accumulation of the plant are drastically affected by high salinity levels in plant root zone. Crop reflectance, chlorophyll fluorescence and nitrogen metabolism are also affected. Early detection of plant stress is very critical especially in intensive production systems in order to minimize both acute and chronic loss of productivity. Until recently, salinity stress management techniques were based on methods such as electric conductivity measurements that indicate the availability of salinity level in the root zone or destructive sampling methods, something difficult to realise in real time. Methods based on plant reflectance evolution do provide direct information about salinity effects detection on greenhouse tomato. Hyperspectral sensing is a non-destructive sensing technology that can be used to extract information from a targeted object based on spectral data. In the present work, the crop reflectance change based on high salinity conditions that measured by portable hyperspectral sensor (ASD Inc.) is studied. For this purpose tomato plants were cultivated under two levels of salinity conditions (electrical conductivity: 2.7 and 5 dS m1). Plant spectral data were transformed into vegetation indices indicative of the physiological status of the plants. The results of the study are promising and show that hyperspectral sensors could be used for early stress detection. T4-P12 DETECTION OF VOLATILE COMPOUNDS IN ACTINIDIA L. BERRIES BY GC-MS METHOD Remigijus Daubaras1, Laima Cesoniene2, Sigita Bogacioviene3, Audrius Maruska4, Mantas Stankevicius4, Algimantas Paulauskas4 1Sodu 7, Vaisvydava, LT-53001 Kaunas, Lithuania; 2Z.E.Zilibero 6, Kaunas, Lithuania; 3Z.E.Zilibero 2, Kaunas, Lithuania; 4Vileikos 8, Kaunas, Lithuania; Nowadays, Actinidia L. genus plants became more and more popular. Actinidia berries are distinguish by many flavour and nutritional qualities, high levels of ascorbic acid and health – promoting effects (Zhang et. al 2001). Aroma is one of the crucial factors that contribute to the berry flavour, which is the result of a subtle mixture of volatile compounds (Zhang et al. 2016; Marsh et al. 2006). The aim of this study was to investigate volatile compounds have in berries of A. deliciosa, A. kolomikta, A. melanandra, and A. arguta. Fresh Actinidia berries were collected in August and September in 2015. Berries were stored at - ºC until analysis. For this research we used gas chromatography-mass spectrometry (GC-MS) method by Mota et al. (2011) with modifications. For analysis berries were used with seeds and peel. Headspace Solid-Phase Microextraction. One commercial fibre was used to extract volatiles. According to the recommendations of 180 III International Symposium on Horticulture in Europe – SHE 2016 the supplier (Supelco, USA), the fibre coated with PDMS/DVB as stationary phases and 65-μm film thickness is the most adaptable to determine the compounds in kiwi matrix. Approximately 1 g of berry sample was homogenized and was loaded in 10 ml vial and then sealed with metal cap and PTFE/silicone septa (ROTH, Germany . Then the fibre was exposed to the headspace for min in ºC. Afterwards, the fibre was pulled into the needle sheath and the SPME device was removed from the vial and inserted into the injection port of the GC system for thermal desorption at °C for minute. All samples were analysed in triplicate. Gas Chromatography/Mass Spectrometry Analyses. HS-SPME analysis was performed using a Shimadzu GC-2010 gas chromatograph and mass spectrophotometer GC-MS-QP2010, and workstation software GC-MS solution version 2.71 (Shimadzu Corporation, Japan). The column used for samples analysis was a RTX-5MS (30 m× . mm× . μm from Restek USA . The injector port was heated to °C. The carrier gas was helium . Aga, Latvia , at a constant flow of , mL/min. The oven temperature was set at °C for min, and then increasing at °C/min to °C and held for min. )onization was maintained off during the fifth minute. Electron ionization detector was at 70 eV. A scan was used from 40 to 400 m/z. The comparison of MS fragmentation pattern with those of pure compounds and mass spectrum database search was performed using National Institute of Standarts and Technology (NIST) MS 08 spectral database. Berries of twelve accessions were analysed. Following volatile compounds were identified after GC-MS analysis: sulcatol, limonene, linalool, sulfurous acid, -terpineol, -pinene, myrcene, phthalic acid, terpenol, sabinene, nerol, neral, oxalic acid, propanoic acid, acetyl valeryl, heptenol, benzoic acid, metyl salycilate, geraniol, and butanoic acid. T4-P13 DETERMINATION OF PESTICIDE RESIDUES IN SWEET FENNEL SEED (FOENICULUM VULGARE MILL.) BY LC-MS/MS AND GC-MS CHROMATOGRAPHIES Nilda Ersoy1, Deniz HAZAR2, Serpil YILMAZ3, Ibrahim BAKTIR4 1Akdeniz University, Vocational School of Technical Sciences, 07058, Campus, 07058 Antalya, Turkey; University, Agricultural Faculty, Department of Horticulture, Antalya, Turkey; 3Akdeniz University, Fisheries Faculty, Department of Basic Sciences, Antalya, Turkey; 4Cyprus International University, Faculty of Agricultural Sci.and Tech , Nicosia, Cyprus 2Akdeniz In this research, the pesticide residues on sweet fennel, Foeniculum vulgare Mill. cv. Dulce, seed grown by good agricultural methods was examined. The sweet fennel was grown in Tefenni County of Burdur Provience in South Western Part of Turkey. The residue analyses were done by GC-MS/MS and LC-MS/MS chromatographies. 188 active substances in used pesticides during the cultivation period were analyzed by LC-MS/MS chromatography meanwhile 116 active substances of pesticides were analyzed by GC-MS/MS chromatography device in sweet fennel seed. This study was conducted in years of 2012 and 2013 consecutively. Detactable pesticide residues have not been measured in the samples. T4-P14 DYNAMIC VS CONSTANT BLUE:RED LED LIGHT RATIO: EFFECTS ON LETTUCE GROWTH AND NUTRITIONAL QUALITY Akvile Virsile1, Ausra Brazaityte1, Giedre Samuoliene1, Ramunas Sirtautas1, Pavelas Duchovskis1, Paulius Vilemas2 1LRCAF Institute of Horticulture, 30 Kauno str., LT-54333 Kaunas distr. Babtai, Lithuania; 2Breslaujos str. 3B, III floor, LT-44403 Kaunas, Lithuania; Previous studies show, that plants are more sensitive to light emitting diode (LED) spectral properties in closed growth chambers, as compared to greenhouse environment, where small flux of natural lighting alleviates the exposure of narrow-bandwidth lighting. In natural environment, plants face a spectral shift of solar light through the day, as the zenith angle changes. Thus, the aim of this study was to evaluate, weather dynamic LED lighting spectra will be physiologically preferable for lettuce plants comparing to constant spectral condition. For that purpose, red and green leaf lettuces Lactuca sativa Green Cos and Red Cos 181 BOOK OF ABSTRACTS were cultivated in closed environment growth chambers / °C, % R( under smart LED lighting unit (LRD, (ortiled, Lithuania . Total photosynthetic photon flux density PPFD of LED lighting was µmol m-2s-1 (18 h photoperiod) and its spectra consisted of red (R) and blue (B) wavelengths. In the first lighting treatment, constant amount µmol m-2s-1) of blue light was applied during the whole photoperiod. In the second lighting treatment dynamic B:R light ratio was applied (8- µmol m-2s-1 of blue light), imitating the relative increase in blue photon flux near solar noon. In both treatments, equal PPFD and daily blue light integral (1,296 mol m-2 day-1) were maintained. The results reveal differential effects of constant and dynamic B:R light ratio on lettuce photosynthesis parameters, growth patterns and antioxidant properties (DPPH free radical scavenging activity, contents of phenolic compounds), nitrate and protein contents. T4-P15 EARLY YIELD PREDICTION IN APPLE ORCHARD Lutz Damerow1, Hong Cheng2, Michael Blanke3 1Institute of Agricultural Engineering, University, Nussallee 5, 531115 Bonn, Germany ; 2Institute of Biol Information Systems, Baoding, Hebei, China; 3INRES-Hort.Science, University of Bonn, Institut fuer Obstbau, Auf dem Huegel 6, 53121 Bonn, Germany The challenge in perennial fruit cultivation is estimating the number and diameter of fruit on a tree as early as possible to achieve yield estimates for farm operations, fruit trade, retailers and storage facilities. Apple recognition algorithms based on colour features are presented to estimate the number of apple fruits and develop early predicting models of apple yield. Fifty cv. Gala apple digital images were captured twice on one, the preferred Western side of the tree row with a variability of between 70 and 170 fruit per tree, under natural daylight conditions at Bonn, Germany. Several image processing algorithms and fruit counting algorithms were used to analyse the apple images in both periods. Finally, an apple recognition algorithm with colour difference R-B and G-R was developed for apple images after June drop, and two different colour models were used to segment apples in the ripening period. The algorithm was tested on 50 images in each period. Close correlation coefficients r2of 0.80 and 0.85 were obtained for two developmental periods between apples detected by the fruit counting algorithm and those manually counted. Two sets of data in each period were used for modelling yield prediction of the apple fruits. In the calibration data set, the r2values between apples detected by the fruit counting algorithm and actual harvested yield are from 0.57 for young fruit after June drop to 0.70 in the fruit ripening period. In the validation data set, the r2-value between the number of apples predicted by the model and actual yield at harvest from 0.58 to 0.71. The proposed model shows a great potential for early predicting yield for individual trees in an orchard. The present results on apple may be applicable to many other fruit crops like Citrus, pear, peach, apricot, kaki, nectarine and almond. T4-P16 EFFECT OF SURFACE COATING WITH WAXES ON THE DORMANCY OF POTATO TUBERS GROWN FROM TRUE POTATO SEED (TPS) Theodora Karanisa1, Alexios Alexopoulos2, Konstantinos Akoumianakis1, Ioannis Karapanos1, Harold Passam1 1Laboratory of Vegetable Production, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece; 2Laboratory of Agronomy, Department of Agricultural Technology, Technological Educational Institute of Peloponnese, 24100 Kalamata, Greece. The control of tuber dormancy is of great importance for long-term storage of potatoes. We studied the effect of postharvest application of two waxes, paraffin wax and the edible wax Teycer shellac, oxidised polyethylene), on the dormancy, weight loss, respiration and soluble sugars (glucose, fructose, sucrose, maltose) content of tubers grown from TPS (hybrid IP88008). Tubers were stored for 120 days after treatment (DAT) in the dark at three different temperatures , , °C and ± % R.(. Paraffin wax and 182 III International Symposium on Horticulture in Europe – SHE 2016 Teycer did not affect tuber dormancy at °C. )n comparison with the control, paraffin-treated tubers had a lower number of sprouted buds after the nd °C and th °C DAT, lower weight loss , °C and lower respiration rate up to the h °C and th , °C DAT. (owever, they presented higher weight loss at °C and a higher respiration rate after the th and th DAT at and °C, respectively. )n contrast, Teycer promoted tuber sprouting after the th °C and the st °C DAT, while tuber weight loss was higher at °C, but not at and °C. )n addition, Teycer increased tuber rot by -32% at all storage temperatures. Teycer -treated tubers had lower respiration rates than the control from 5 to 15 DAT °C and up to the th °C and the th °C DAT; however respiration was higher than the controls after the th °C and the th , °C DAT. Compared with controls, the fructose, glucose, sucrose and maltose content was higher in paraffin-treated or Teycer -treated tubers at all storage temperatures. We conclude that paraffin wax effectively controls tuber dormancy at οC, whereas the application of Teycer is not satisfactory for the control of tuber dormancy. T4-P17 EFFECTS OF ELECTRIC POWER SUPPLY FOR ARTIFICIAL ILLUMINATION OF GREENHOUSE CROPS AT VOLATILE CURRENT SUPPLY Tundra Ramirez, Peter Kläring Theodor Echtermeyer Weg 1, Grossbeeren, Germany; In Germany, the increasing generation of electricity based on renewable energy is accompanied by a high volatility in the current availability. This requires not only flexible capacities for current production, but also for current use and results in a high variability of the electricity tariff. Greenhouses could be such flexible users of volatile current supply. They can use it for heating the greenhouse and illuminating the crops. The present study considers the use of volatile current supply for the artificial illumination of greenhouse crops. In a model study, the effect of illuminating a tomato crop on photosynthesis as well as the potential yield at different times of the day and of the year is assessed. In addition, the lamps release heat into the greenhouse and thus decrease the heat energy consumption. The photosynthesis model was evaluated for the application of artificial light using measurements in a greenhouse on complete crops. Simulated scenarios show the effects of variable artificial lighting. Obviously, the effect is higher at night and during winter time compared to summer. However, even during bright summer days, a photosynthesis driven model generates a slight increase in yield. The aspect of deriving the crop yield directly from the crop photosynthesis is discussed. This study was supported by a grant of the Landwirtschaftliche Rentenbank with the assistance of the Federal Agency for Agriculture and Food. T4-P18 EFFECTS OF KAOLIN AND SHADING TREATMENTS ON FRUIT DEVELOPMENT AND COLOURATION OF POMEGRANATE (PUNICA GRANATUM L.CV. HICAZNAR) Keziban Yazici 1, Lami Kaynak 2, 1 Recep Tayyip Erdogan University, Faculty of Agriculture and Natural Science, Horticultural Department, Rize, Turkey 2 Akdeniz University, Faculty of Agriculture , Horticultural Department, Antalya, Turkey; Pomegranate is mainly produced in Mediterranean (%53), Aegean (%20) , and Southeast Anatolia (%8.4) regions depending on climatic requirements in Turkey. Hicaznar, is the most important pomegranate fruit cultivar produced and exported from Turkey. This variety has been appreciated in Europe, because of its red skin colour and red aril which resulted in increasing export. In addition to its high yield, its conformity with transportation and storage make it more attractive in the markets. High Temperatures affect fruits development and cause yield and quality losses in pomegranate production area in the Mediterranean region of Turkey. In this study, the effect of 35% shading and 3% kaolin applications on fruits development at three 183 BOOK OF ABSTRACTS different periods were investigated. 45 fruit in each applications and a total of 360 fruit in different four direction of each tree and inside of the crown were determined and labeled before the temperature increase. Fruit width and fruit length development was measured regularly by compass in these fruits at two week intervals (July 30, August 15, August 30, September 15, October 6) until the harvest. Skin colour measurements were determined using a Minolta CR-200 chroma meter in harvest. The results indicated that kaolin application, shading and their interaction were not statisticly important for fruit development in this study in which the effects of kaolin and shading treatments on fruit development of pomegranate (Punica granatum L.cv. Hicaznar) were examined for two years, However, the fruit color was significantly increased with kaolin applications. T4-P19 EFFECTS OF LOW CHILL ACCUMULATION ON DORMANCY DYNAMICS OF DIFFERENT APPLE TREES CULTIVARS Igor de Albuquerque, Paulo Mello-Farias, Robson Ryu Yamamoto, Ana L’cia Soares Chaves, Flavio Gilberto Herter Federal University of Pelotas, Faculty of Agronomy Eliseu Maciel, Pelotas-RS, Brazil The experiment was developed in an orchard located in Southern region of Brazil. The objective of this research was to evaluate the behavior of different apple trees cultivars grown under mild winter conditions. )t was used apple trees cultivars with different chilling requirements: Eva low , Castel Gala medium and Golden Delicious high . Two biological tests to determine the dormancy dynamics were performed: the Tabuenca s test, which determine the water content inside buds and biological test to determine depth dormancy. To execute those tests, long and short branches of each cultivar were regularly collected during winter period of 2015. The dormant stadium in vegetative buds was evaluated by the single-node test and in floral buds by the Tabuenca s test. Dormancy process is affected by the lack of cold temperatures. The high temperature range during the dormancy period did not allow the cultivars to reach a deep stage of dormancy, in this way it is difficult differentiate each phase. The results demonstrate that all cultivars presented superficial dormancy (ecodormancy) through single node test during the whole winter, and the bud bursting capacity is attached to favorable growth conditions. This research may be helpful for future achievements in tree phenology field under mild winter conditions and to select adapted cultivars. T4-P20 EFFECTS OF PLANT DENSITY AND THE NUMBER OF EMITTERS ON PLANT GROWTH AND NITRATE CONCENTRATION IN SPINACH CULTIVATED IN SUBSTRATE Rui Machado1, Isabel Alves-Pereira2, Rui Ferreira2 2)CAAM, 1Dept. De Fitotecnia, Univ.de De Evora, 7000 Evora Codex, Mitra, Portugal; Universidade de Évora, )CAAM, Universidade de Évora, )CAAM, Universidade de Évora, - Évora, Portugal The effects of plant density and the number of emitters per Styrofoam box on plant growth and nitrate (NO3) concentration were evaluated in spinach (Spinacia oleracea L. cv. Tapir). Spinach seedlings were transplanted at 45 days after emergence into Styrofoam boxes filled with the substrate and were grown during winter in an unheated greenhouse with no supplemental lighting. The experiment was carried out with four treatments, including two plant densities (160 and 280 plants/m2) and two number of emitters per Styrofoam box (4 and 8 emitters). Each planting box was irrigated daily and fertigated with a complete nutrient solution. Shoot dry weight was not affected by plant density. However, yield increased with plant density and emitter number. Leaf-blade NO3- concentration was not affected by the interaction between plant density and number of emitters, but petioles NO3- concentration was greater in treatment with 160 plants/m2 and 8 emitters. Although leaf-blade NO3- concentration was not affected by plant density, it decreased with the number of emitters. On the other hand, petiole NO3- concentration was not affected by 184 III International Symposium on Horticulture in Europe – SHE 2016 plant density or number of emitters. Leaf-blade NO3- concentration ranged from 3.2 to 4.1 mg/g fresh weight, occurring the highest value in the treatment with 280 plants/m2 and 4 emitters. Petiole NO3concentration ranged from 3.5 to 5.3 mg/g fresh weight, values that were higher than allowed by EU regulation. T4-P21 EFFICIENT VEGETATIVE PROPAGATION OF VARIOUS CLONES OF MASTIC TREE (PISTACIA LENTISCUS VAR. CHIA) THROUGH ROOTING OF SHOOT CUTTINGS Stefanos Kostas 1, Efstathios Hatziloukas 2, Stefanos Hatzilazarou 1, Athanasios Economou 1 1 Dept. of Horticulture, School of Agriculture, Aristotle University, 54124 Thessaloniki, Greece 2 Dept.of Biol. Applications andTechnologies, University of Ioannina, 45110 Ioannina, Greece Pistacia lentiscus var. Chia (mastic tree), which produces the resin or mastic from its wounded trunk, is characterized as a difficult-to-root plant species. It is cultivated in Greece exclusively on the island of Chios and is propagated by burying in the ground of the field halfway 40-50 cm shoots, a practice however with a low percentage of rooting, time-consuming and limited efficiency in expanding new plantations or replacing those that were struck by fires. The aim of this work was to develop a protocol for mass production of young plants vegetatively, through shoot cuttings. From five very common clones, of high productivity in resin mastic , named Mavroschinos , Votomos , Maroulitis , Fardis and Psilofyllos , semi-hardwood leafy cuttings, 10-12 cm long, were cut (in winter, spring, summer and autumn) and treated with K-IBA (potassium salt of indole-butyric acid) at concentrations of 0, 4.000, 8.000 and 16.000 ppm in water solutions. Cuttings were then planted on perlite-peat mixture (1:1 v/v) in boxes and transferred to fog system for rooting. The effect of rooting medium was also studied by using various mixtures of perlite and peat (1:0, 2:1 and 1:1, v/v) in combination with CaCO3 (0, 10 or 20 g/l). Statistical analysis of the data revealed a significant increase of rooting with 4.000 and 8.000 ppm of K-IBA, with maximum rooting in winter (80%) and minimum in spring (17.5%). K-IBA application further increased the number and length of roots in comparison with the control. The use of plain perlite in combination with the addition of 20 g/l CaCO3 also increased the rooting rate of cuttings. Plant genotype influenced rooting ability of cuttings since large differences were observed among clones, with highest rooting rates found in clone Psilofyllos %, followed by Mavroschinos . % , Votomos . % , Fardis % and Maroulitis %. T4-P22 ESTIMATED CO2 SEQUESTRATION CAPACITY OF MAPLE (ACER SP.) AND LINDEN (TILIA SP.) TREES K|roly (rotkó, Mark Steiner, Magdolna S“töri-Diószegi, M|té Vértesy Szent )stv|n University, Faculty of (orticultural Science, Department of Floriculture and Dendrology, 53, Hungary Budapest Pf Urban trees also sequester considerable amount of CO2, however, there are little and sometimes contradicting data on this topic. Previous results suggested that there are large differences between species and cultivars depending on the site and environmental conditions too. Measuring the leaf gas exchange over longer periods by taking appropriate leaf samples showed specific diurnal course of CO2 fixation, depending on the actual solar radiation, stomatal conductance and further environmental conditions, like water and nutrient supply. In our research program leaf gas exchange measurements were carried out on maple (Acer sp) and linden (Tilia sp) cultivars over three years, measuring leaf samples from 6:00 am to 20:00 pm repeatedly in every two hours. Using the measured data we calculated the daily course of CO2 fixation and the daily capacity of leaves. Further on the measurements on canopy leaf area allow estimation of daily sequestered CO2. Leaves of linden cultivars assimilated daily 5 – g·m− CO , while the daily sequestration capacity of maple leaves was between 4 – g·m− . These data confirm the large differences between different species of urban trees and provide data for further calculation of CO2 sequestration capacity, which 185 BOOK OF ABSTRACTS is one of the important environmental benefits of urban trees. However, we are aware that estimation of environmental benefits of urban trees in a complex model requires further in situ measurements on LAI and canopy volume. T4-P23 EVALUATION OF THE BIODEGRADATION RATE OF MULCH FILMS Paulo Fortes Neto, Nara L’cia Perondi Fortes, Gustavo Souza, Arthur Saraiva, Raquel Costa, Elizabeth Duarte Av. )t|lia TAUBATÉ V)LLAGE R RUA , N , SO PAULO,BRAΒ)L, Taubaté-12, Brazil; In Brazil were used in 2012 about 2,500 tons of mulch films on horticulture. After the crop cycle, polyethylene mulches are often buried or burned with crop residues, once mulch films recycling is timeconsuming and expensive due to high removal labour cost. The biodegradable mulch films appear as an alternative to the polyethylene use, once they are incorporated in soil with crop residues at the end of crop cycle. This study was performed in order to evaluate the biodegradation of different types of mulches in both real and laboratorial conditions, in tropical soils. The experimental design used was randomized blocks with five repetitions and four treatments: bare soil (BS); rice husk mulch (RHM); polyethylene mulch (PM) and biodegradable mulch (BM). At real conditions, the mulch samples were buried at 20 cm depth and were collected after 60 days, being the biodegradation determined by the lost weight. In laboratory a respirometric test were performed with the same soil from real conditions test, at 70 % of water holding capacity and ºC. Carbon dioxide production was measured every days, during a twenty days period. Microbial carbon was also determined by fumigation method, at the end of respirometric test. The results showed that the BM modality produced more carbon dioxide, , µg g soil-1, than other modalities. Microbial carbon was higher on RHM modality with . µg g soil- , followed by BM with . µg g soil-1 and lower values were found on BS and PM, with . e . µg g soil-1respectively. From the results obtained, RHM and BM higher CO2 verified were probably due to a synergetic effect between mulches carbon content and microorganism growth rate. T4-P24 FLUORESCENCE INTENSITY IN APRICOT LEAVES UNDER THEIR DEHYDRATION Valentina Gorina1, Vadim Korzin2,Nataliya Mesyaz2, Yurij Ivashchen2 1Crimea, 2Nikita, Yalta, Russian Federation; Yalta, Russian Federation. The aim of researches was to study the chlorophyll fluorescence intensity of apricot leaves in the process of dehydration and identification of cultivars hybrids with high potential drought resistance. For the experiment were take leaves of two cultivars of apricot Kryumskij Amur - control and Bukuriya and hybrid 84-929. Chlorophyll fluorescence was measured on a portable fluorometer "Florotest". Was registered initial level of fluorescence after irradiation (F0), the maximum (Fm) and stationary (Fst) values after light adaptation Stirber, . Studies were carried out in August the average air temperature was . ° C, the amount of rainfall - 19.4 mm). Short dehydration (4 hours) was resulted in an increase in fluorescence parameters. Significant differences were observed in cultivar Kryumskij Amur. A low variation of values were observed in Bukuriya cultivar and hybrids 84-929. Prolonged dehydration (24 hours) caused a significant decrease in all studied parameters. The lowest values (Fv=Fm - F0 – . ; ФА = Fm -Fst)/Fm – 0.17; vitality index = Fm / Fst – 1.21) were observed in 48.8% loss of water in the leaves of cultivar Bukuriya. A turgor of the leaves of this cultivar after a long wilting has not recovered. Was revealed a essential correlation (0.7-0.8) between the calculated parameters of fluorescence and water content in the leaves. In the control cultivar Kryumskij Amur after 24 hours of wilting leaves lost 45.2% of water and recovered only 7.5%, the fluorescence parameters were low (Fv – . ; ФА - 0.23; vitality index - 1.3). Hybrids 84-929 after prolonged dehydration showed the highest value of the variable fluorescence (1098.7 rel. u.). After the 186 III International Symposium on Horticulture in Europe – SHE 2016 restoration of 45% leaves these options increased to 0.54 and 2.16 respectively. Cultivar Kryumskij Amur and hybrid 84-929 have an increased drought resistance. Acknowledgement: This study was funded by a research grant № Foundation. -50-00079 of the Russian Science T4-P25 HOW TO EVALUATE DAMAGES CAUSED BY THE SPOTTED WING DROSOPHILA ON NATIONAL LEVEL? Esther Bravin1, Felix Gremminger2 1Agroscope Changins-Wädenswill ACW, Postfach, Waedenswill, Switzerland; 2Schloss, Wädenswil, Switzerland. 2011 the spotted wing drosophila, Drosophila suzukii, has been for the first time detected in Switzerland (Baroffio & Fischer, 2011). The following years, the spotted wing drosophila spread out rapidly and caused serious economic damages in 2014 (Kuske et al, 2014). However, because of large differences in the damage degree between host plants and regions it was difficult to evaluate the economic damages on national level. 2014 different regional offices in Switzerland started their own survey to determinate the damage for stone fruits (mainly cherries and prunes , berry and grapes for wine production . The surveys weren t harmonized and it was not possible to have an overall picture on national level. For those reasons, we used these surveys, including feedback and recommendation from the regional offices, to create a new survey on national level. The aim of this survey was to analyse the plant protection strategies of fruit and wine farmers and to evaluate the damages for each cultivar caused by the spotted wing drosophila. For cherries, prunes, berries and wine grapes we developed a separate survey. The survey has been composed using the online survey-tool q-set . Regional offices for horticulture sent the survey to fruit and grape growers in their region, during several months they could answer to about 30 questions (depending on the cultivar). The survey for cherry had a rate of return of about 11% (111 filled questionnaires). The group of the German speaking regions had a higher rate of return than the group of the French speaking regions (108/3). Due to the hot and dry summer, 2015 was not a year with high damages caused by the spotted wing drosophila (news.admin.ch). However, there was some damage in cherries, with a damage rate of 10 % in the supply of the cultivar Kordia and 20% in the cultivar Sweetheart. The following steps of this research will be to analyse more data, damages, the plant protection strategies used by fruit and wine growers against the spotted wing drosophila and their relationship for all stone fruits, berries and wine grape. T4-P26 HYPOCHLORITE APPLICATION IN HYDROPONICS WITH ALL ITS BENEFITS AND HAZARDS FOR TOMATO PRODUCTION Dennis Dannehl1, Ingo Schuch1, Raquel Salazar2, Uwe Schmidt1 2Autonomous 1Humboldt - Universität zu Berlin, Albrecht-Thaer-Weg 3, 14195 Berlin Berlin, Germany; University of Chapingo, Km 38.5 Carr. Mexico-Texcoco , Chapingo, Edo. Mexico, C.P 56230 , Texcoco, 56230, Mexico. Besides physical disinfection methods, chemicals used to reduce the occurrence of pathogens and the application of pesticides in greenhouses are increasingly gaining in importance. However, the knowledge about required amounts to achieve a high efficacy against pest without getting phytotoxic problems or to ensure that by-products of disinfectants (e.g., chlorate) do not exceed the maximum residue levels (MRL) in fruit is scarce. Therefore, the research was focused on the identification of interactions between hypochlorite used as a disinfectant for a recirculating tomato production system and the algae formation, spread of microorganisms, plant characteristics and fruit quality. As such, potassium hypochlorite (1% KClO) was supplemented ones a week for 90 min into different nutrient solution tanks until a free chlorine concentration of 1 mg/l (DI) and 2 mg/l (DII) were reached, respectively.Results showed that the formation 187 BOOK OF ABSTRACTS of the algae biofilm was reduced by 15% (DI) and 48% (DII), where the microorganisms were suppressed by up to 100% as well. Plants exposed to treatment DI showed a comparable plant height to the control plants, whereas this plant parameter was significantly increased by 12 cm caused by treatment DII after a growing period of seven weeks. However, the formation of leaves was more pronounced by treatment DI, which was the main reason for the formation of more trusses and associated tomato fruit. An increased yield by 10% (DI) was calculated compared to the control. While phytotoxic problems can be excluded, the chlorate content in tomato increased from 0.01 mg (Control) to 0.22 mg (DI) and 0.25 mg ClO3-/kg fresh weight. This enhancement might be considered as critical value because MRLs will be defined 2016. The levels of carotenoids increased with increasing concentrations of hypochlorite as well, which was probably based on a stress response owing to the accumulation of chlorate. T4-P27 IMPACT OF BUDLOAD AND IRRIGATION MANAGAMENT ON YIELD, BERRY QUALITY AND PHENOLIC COMPOSITION OF CV. ALPHONSE LAVALLEE Semih Tangolar, Serpil Tangolar, G“zin Tarim, Melike Ada Cukurova Universty, Faculty of Agriculture, Department of Horticulture, 01330 Adana, Turkey In this research, the effects of two irrigation regime (IR-1 and IR-2) and bud load (BL) treatments on yield, cluster and berry quality and phenolic composition of Alphonse Lavallee table grape variety were studied in 2013-2015 years. The midday leaf water potential tresholds were used for irrigation timing and those values were set as -1 MPa until flowering, -1.3 MPa between fruit set- veraison and veraison-ripening periods, and 1.2 MPa after harvest for both IR treatments. Water volumes applied in irrigations to the IR-1 plots were 50% and 75% of the cumulative evaporation from the Class A pan between the growth stage berry settingveraison and veraison-ripening, respectively, while those portions for the IR-2 treatment was vice versa. Both IR treatments and the non-irrigated treatment have been examined for two bud loads based on mean pruning weight; 1BL) Normal bud load (20 buds for the first 500 g pruning weight and then 10 buds for each additional 500 g), 2BL) Double amount of 1BL. Grape yield and cluster weight were slightly higher in irrigated and higher bud load vines. But, no significant change was detected in berry weight, total soluble solids, acidity and pH. Pruning weight was greater in the irrigated and 1BL treatments than in those of rain-fed and 2BL vines. Total ten anthocyanin compounds were detected in berries. The highest value of total anthocyanin content were obtained from the grapevines grown under rain-fed conditions and followed by the IR-2 treatment. Related with the non coloured phenolic (NPC) compounds, two flavanols, six phenol acids and four flavonols were identified and quantified in berries. All these compounds were affected from treatments in either year of the study. The level of total NPC has increased by non-irrigated and 2BL treatments in both years. Finally, results show that while maintaining biochemical composition of the berries, grape yield can be increased via sufficient irrigation and higher bud load application. 188 III International Symposium on Horticulture in Europe – SHE 2016 T4-P28 IMPACT OF NITROGEN FORM AND SUPPLY LEVEL ON GROWTH, YIELD AND NUTRITIONAL VALUE IN HYDROPONICALLY-GROWN STAMNAGATHI (CICHORIUM SPINOSUM L.) Dimitrios Savvas1, Martina Chatzigianni1, Baraa Alkhaled2, Aristidis Stamatakis2, Ioannis Livieratos2 1 Agricultural University of Athens, Laboratory of Vegetable Production, Iera Odos 75, 11855 Athens, Greece 2 Mediterranean Agronomic Institute of Chania, Alsyllio Agrokepio, 1 Makedonias str, 73100 Chania Crete , Greece The objective of this study was to determine the impact of total-N concentration and the ratio of -NH4 to total-N on growth, yield, and quality of two stamnagathi (Cichorium spinosum L.) landraces (Tavri and Stavros), originated from two different Cretan habitats (mountainous and seaside) and grown in an open hydroponic system using perlite as substrate. Six different nutrient solution (NS) treatments were tested by combining two levels of total-N supply (4mM N or 16mM N) with three levels of ammonium to total-N supply ratio (Nr) in the NS (0.05, 0.25 and 0.5, corresponding to supply of 5%, 25%, 50% of total-N in the form of NH4-N, respectively). The results showed that the yield in terms of above-ground fresh and dry weight per plant was unaffected by NS treatments at both seed origins. However, Tavri plants showed higher dry matter content compared with that of Stavros plants. No significant differences were observed in the leaf chlorophyll content of leaves, although increasing both total-N supply and Nr in NS enhanced significantly the concentration of organically-bound nitrogen in the leaves. The concentrations of K and Ca were significantly reduced by increasing Nr from 0.05 up to 0.5. Additionally, the concentration of leaf K was further reduced by increasing the total-N supply. In contrast, the Mn and B concentrations in leaves increased significantly with increasing Nr in NS. With respect to stamnagathi leaf quality, increasing total-N supply markedly increased leaf nitrate content (NO3-N) but the differences were significant only in Stavros. However, increasing Nr significantly decreased the nitrate content with the lowest content occurring at the highest Nr level. Interestingly, Stavros plants exhibited higher nitrate contents than those of Tavri plants at all treatments. Moreover, the results revealed a superiority of Tavri over Stavros plants in total flavonoids, phenols and antioxidant activity. T4-P29 IMPROVING COLOUR FORMATION IN HORTICULTURE FRUIT CROPS Henrike Schuhknecht1, Michael Blanke1, Lutz Damerow2 1Institut fuer Obstbau INRES-Hort Science, University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany; of Agricultural Engineering, University of Bonn, Nussallee 5, D-53115 Bonn, Germany. 2Institute Climate change brings about warmer autumn temperatures at the time of colouration viz anthocyanin biosynthesis in the peel of many fleshy fruit crops, including apple, pear, apricot or nectarine fruit. Anthocyanin de-novo biosynthesis is triggered by a combination of light (PAR and UV) and low temperatures or large day-night temperature gradients. The causes of insufficient fruit colouration hence include less light due to decreasing light intensity, a shorter photoperiod and a lower solar angle in the autumn plus lack of cool temperatures. Five-year-old apple cv. Braeburn (ilwell trees, grafted on M rootstock were employed at the experimental orchard at Campus Klein-Altendorf, University of Bonn °N with a spacing of 3.5 m x 1.3 m. The objective of the study was to improve fruit colouration by both chemical and reflection material.Hence, two biostimulants, WUXAL ATriun (1.5L + 3 L/ha) or SunredTM(2.5L + 5 L/ha) were applied twice 5 and 3 weeks before commercial harvest.Two white reflective mulches, ExtendayTM (80 g/m2; Extenday Co, WA, USA) and Lumilys (Beaulieu, Belgium) were spread (3 m wide) on 27 August 2015 in the alleyways on both sides of the tree rows. Two untreated viz unsprayed plots served as control. Each treatment comprised of ten trees with border trees either side. SunredTM is a new biostimulant, which contains the following compounds for four modes of action: i) phenylalanine as base for PAL viz anthocyanin synthesis, ii) methionine as precursor of the ethylene pathway and ripening iii) oxylipins to enhance pigment performance and reduce plant stress and iv) monosaccharides as carbon skeletons and energy provider. Light measurements showed a several-fold increase in light reflected from both plastic mulches, Extenday 189 BOOK OF ABSTRACTS and Lumilys, particularly into the lower part of tree canopy. The individual colour measurements on attached fruit showed significantly improved colour (larger a value) of the down-facing side of the apples with reflective mulch, resulting in a better class I classification and better financial returns. Overall, the experiments showed a major improvement of colouration and sugar (the largest with Lumilys) with both mulches, and a somewhat improved colouration with one of the chemicals, the biostimulant SunredTM, which maintained fruit firmness with a trend of 3.5% increase in sugar. T4-P30 IN VITRO PLBS ORGANOGENESIS OF PHALAENOPSIS TO CARBOHYDRATE SOURCES UNDER LED LIGHTS Mehraj Hasan 1, Meskatul Alam 2, Sultana Umma Habiba 3, Kazuhiko Shimasaki 2 1 The United Graduate School of Agricultural, Ehime University, 3-5-7 Tarami, Matsumaya, Ehime 790-8556, Japan 2 Kochi University, B200 Monobe, Nankoku 780-8520, Japan 3 Ehime University, 3-5-7 Tarami, Matsumaya 790-8556, Japan Throughout of this study, our vision was to find out the impact of LED and effective carbohydrate source for the in vitro regeneration of the PLBs (protocorm-like bodies in Phalaenopsis Fmk . We allowed fifteen LED treatments with three carbohydrate sources in our study. Performance of PLBs production was influenced diversely at different carbohydrate source under different LED. We found sucrose as the best carbohydrate source when it was placed under red + white LED for the in vitro organogenesis of PLBs i.e., mean number of PLBs (54.13) and their fresh weight (0.109 g) followed by white florescent tube (control) in Phalaenopsis in culture medium. On the other hand, trehalose carbohydrate source showed best performance under blue + white LED in terms of number of PLBs (36.33). Organogenesis of PLBs in culture medium was very poor in the carbohydrate source maltose than sucrose and trehalose. The result of this study suggested using sucrose as carbohydrate source and putting under red + white LED (chronologically half of the entire culture period for the better PLBs regeneration of Phalaenopsis Fmk . T4-P31 INCIDENCE OF BEMISIA TABACI AND TRIALEURODES VAPORARIOURUM IN HYDROPONIC TOMATO CROP AS AFFECTED BY ROOTSTOCK Smiljana Goreta Ban1, Katja Zanic2, Gvozden Dumicic2, Gabriela Vuletin Selak2, Slavko Perica2 1Institute 2Institute of Agriculture and Tourism, Karla Huguesa 8, Pore269, Croatia; for Adriatic Crops and Karst Recl, Put Duilova 11, Split, Croatia; Katja.Zanic@krs.hr Vegetable growing in greenhouses is among the most advanced agricultural technologies. Constant improvement of production technology in order to achieve profitability and contribute to environment protection is the main goal and implies new approaches. Tobacco whitefly Bemisia tabaci (Gennadius) and greenhouse whitefly Trialeurodes vaporariorum (Westwood) are the most widespread and damaging whitefly species worldwide, and both of them habit on tomato. The aim of this study was to determine the effect of rootstock on incidence of adult instars of B. tabaci and T. vaporariorum. Two separated greenhouse experiments were conducted in autumn 2015, using tomato cv. Clarabella as a scion, in a hydroponic system. In both experiments, grafted plants (cv. Clarabella grafted onto the commercial rootstocks Arnold, Buffon, Emperador, and Maxifort) were compared to non-grafted plants (cv. Clarabella and rootstocks) and selfgrafted (cv. Clarabella grafted on its roots) plants. B. tabaci population density (number of adult individuals per leaf) was lower on rootstock leaves and on leaves of cv. Clarabella grafted on tested rootstocks compared to non-grafted or self-grafted cv. Clarabella These results indicate that the effect of rootstock on B. tabaci incidence was transferred into scion at 2, 5 and 7 days after infestation (DAI). Such clear effect was found for number of T. vaporariorum adult individuals per leaf only in cv. Clarabella grafted onto the rootstock Buffon or on Buffon leaves compared to self-grafted tomatoes at three sampling dates (2, 5 and 7 DAI). This study suggests that rootstock effect on pest incidence could be transferred in scion but effect depends on rootstock 190 III International Symposium on Horticulture in Europe – SHE 2016 and pest. Also, it confirms the importance of tomato rootstock choice and that grafting could be applied in a sustainable management against B. tabaci and T. vaporariorum in tomato crops. T4-P32 INDUCTOME: OPTIMIZING PLANT PERFORMANCE UNDER CONTROLLED STRESS CONDITIONS AIMING TOWARDS INCREASED ACCUMULATION OF SECONDARY METABOLITES IN TOMATO LEAVES Simone Schmittgen, Tanja Groher, Mauricio Hunsche, Georg Noga INRES-Horticultural Crops, Auf dem Huegel 6, 53121 Bonn, Germany. InducTomE is an interdisciplinary and translational research project focusing on the accumulation of secondary metabolites (SM) in by-products of tomato cultivation under controlled stress conditions. Further aims are to establish the practical implementation of SM extraction as well as to evaluate the economic chain, from the supplier of raw material to the industrial partner enabling the use of the compounds e.g. in the food or health care segments (www.biosc.de/inductome). The challenge in commercial-like greenhouses is the establishment and control of growing conditions that ensure highest fruit yield and quality but at the same time allowing for example inducing the accumulation of SM in waste-materials such as pruned leaves, sidebranches, stems and plant residues (at the end of the cultivation cycle). In preliminary experiments, we adopted traditional physiological parameters (e.g., gas exchange, vegetative and generative parameters) and biochemical indicators (e.g., contents of chlorophyll and proline, osmotic potential) to evaluate the performance of plants growing under stress situations. In this context, we identified that nitrogen supply, temporary water deficit and supplemental lighting are promising treatments to increase contents of SM in leaves. Nevertheless, intensity and timing of the treatments still has to be optimized in order avoid fruit yield loss, the primary income source of the growers. Currently, optical sensors with high temporal and spatial resolution (for example multiparametric fluorescence) are in use to provide a fast and non-destructive evaluation of the plant responses to the treatments applied as single, combined or sequential stresses. Physiological parameters will be related to the accumulation of selected secondary metabolites (e.g., solanesol and rutin in the leaves. The industrial utilization of huge plant waste amounts originated from the commercial cultivation of tomatoes might support higher income and sustainable development of the cross-border bio-economy. T4-P33 INFLUENCE OF DRIP IRRIGATION ON PEACH TREE GROWTH IN THE CHERNOZEM OF DOBROGEA, ROMANIA Leinar Septar1, Cristina Moale1, Cristian Paltineanu2 1 Research Station Fruit Growing Constanta, No. 25 Pepinierei Street, Valu lui Traian, 907300 Constanta, Romania 2 Marasti Boulevard 61, Bucharest, 011464 Bucharest Bucharest, Romania In arid and semi-arid regions, irrigation should increase water use efficiency and decrease the impact on the environment, specifically in the context of global change. The split-plot experiment described here is bifactorial: 1) with irrigation strategy having three graduations, and 2) two peach cultivars, namely 'Southland' and 'Cardinal', three years old. The irrigation regime consists of a fully irrigated treatment (T1, non-stressed) according to the irrigation needs (100% of ETc = ETo x Kc, Penman-Monteith method), a deficit irrigation treatment (T2) irrigated with half the amount of water in T1 (50% of ETc), and a control, non-irrigated treatment (T3). These plots comprised three adjacent fruit tree rows, with the central row containing five trees for measurements and observations. Soil water potential was measured with Watermark resistance blocks installed at four depths: 20 cm, 40 cm, 60 cm and 80 cm at a 1m distance from the tree trunk, with two replicates for each tree. The paper describes peach growth in a chernozem of Dobrogea region, Romania. The study was carried out during three years (2010-2012). Irrigation application induced a significant increase in annual shoots in T1 versus the other two treatments, especially in 'Southland' cultivar, under the specific soil 191 BOOK OF ABSTRACTS and climate conditions of the region. The anticipated shoots and tree volumes were also different, depending on the irrigation amount. T4-P34 INVITRO PROPAGATION OF `JUMBO´ BLACKBERRY CULTIVAR Nesibe Ebru Kafkas, Sina Kefayati, Salih Kafkas Department of Horticulture, Faculty of Agriculture, TR-01330 Adana Balcali, Turkey Blackberries, along with red and black raspberries and their hybrids, are fruiting plants belongs to the Rosaceae family, Rubus (Tourn) L. Genus, a shruby tree with erect, semierect or creep grown habit, and most cultivars have thorny stems. Thornless blackberries are commercially propagated by tip layers or stem cuttings. Micropropagation methods are become very important and having much more advantages compare to the classical propagation methods. In Turkey, blackberries are propagated by hard and soft wood cuttings, by layering and bush division and more commonly using softwood and wood cuttings. In this study, it was aimed to optimize a protocol for in vitro propagation of Jumbo blackberry cultivar using various plant growth regulators. T4-P35 IODINE BIOFORTIFICATION OF GREENHOUSE CUCUMBERS CULTIVATED IN A HYDROPONIC SYSTEM Diemo Daum, Mathias Becker, Benedikt Heitmann, Patrick G. Lawson University of Applied Sciences Osnabrueck, Emsweg 3, Faculty AL, Oldenbruger Landstr. 24, 49090 Osnabrueck, Germany; Iodine is an essential micronutrient for humans, which is often insufficiently ingested with food. Agronomic iodine biofortification of food crops by soil-applied iodine fertilizers was proposed as a new strategy to address iodine deficiency. However, under field conditions the efficacy of this approach is often limited, because once in the soil, iodine can rapidly be converted to non-phytoavailable forms (e.g. by sorption and volatilization processes) or leached below the root zone of plants. In this regard, soilless systems under protected cultivation may provide advantages. Therefore, in this study we investigated different iodine biofortification approaches on greenhouse cucumbers grown in a rockwool hydroponic system. In the first greenhouse experiment, the dissolved iodine fertilizer was dripped into the growing medium with the supplied nutrient solution or, alternatively, was repeatedly sprayed on the shoot of the plants. KI and K)O were used as iodine fertilizer. K) concentrations above µmol )/L in the root-applied nutrient solution resulted in a distinct reduction of the crop yield, while in all other treatments the fruit development remained unaffected. With both tested fertilization techniques the iodine content of the fruits could be increased above µg ) per g FM. The second greenhouse experiment was conducted at a vegetable farm. Iodine was supplied via the nutrient solution containing KI or KIO3. With rising KI concentration, the iodine content of the fruits increased linearly without decline in crop yield or quality. At the highest KI concentration, the iodine content in fruits amounted to µg ) per g FM. A slightly higher iodine content was achieved at the highest KIO3 concentration. The iodine accumulation in cucumbers was related to the climatic conditions in the greenhouse during the cultivation period. When global radiation and air temperature rose, the iodine content in fruits increased. 192 III International Symposium on Horticulture in Europe – SHE 2016 T4-P36 MODERN TRAINING SYSTEMS FOR SWEET CHERRIES -SLENDER SPINDLE OR HEDGEROW FOR PROTECTED GROWING?Verena Overbeck1, Michael Blanke1, Michaela Schmitz2 1 Department of INRES (orticultural Science, University of Bonn, Auf dem (“gel , Bonn, Germany 2 Department of Applied Science, von-Liebig-Strasse 20, 53359 Rheinbach, Germany Recent climate change requires crop protection against heavy rain and hail, but also offers new opportunities for horticultural production. While polytunnels and hail nets protect the crops against adverse weather, crops can be forced and harvested, irrespective of the weather. Moreover, the modified microclimate may increase yield as well as the fruit quality. In sweet cherry, growing trees in a closed polytunnel forces the trees and reduces fruit cracking. This environmentally-friendly approach without fossil fuel enables customers to purchase domestic products with good taste, firmness and carbon footprint as well as good returns to the grower before the field season starts. One objective of the study was to determine the influence of the modified microclimate on flowering and harvest date. Another objective was to compare different planting systems and pruning measures with regard to yield and the efficiency on small cultivation areas. In 2008- , three early cherry cultivars Samba , Bellise and Rita on dwarfing rootstock GiSelA were grown at Campus Klein-Altendorf latitude . °N , University of Bonn, Germany. Each research block consisted of 2 double row beedings with a slender crown (system 1: 2.70 m x 2.00 m; system 2: 1.75 m x 2.9) and a hedgerow system ((2.4 m x 2.0) x 1.5m). Half of the trees were grown without cover as control. The pruning of the beedings was made by hand and that of the hedgerow mechanically. The harvest date was advanced by 9-19 days; yield was 7-12 t/ha in system 1 (1027 trees/ha), 8-12 t/ha in system 2 (706 trees/ha) and 13-18 t/ha in the hedgerow (2000 trees/ha). In consequence, the small planting density enables a proper yield on small areas. The use of mechanical thinning signified a substantial time and workload saving. T4-P37 MULCHING COMBINED WITH REGULATED DEFICIT IRRIGATION CAN BE SUCCESSFULLY APPLIED TO YOUNG PEAR TREES Lavdim Lepaja1, Endrit Kullaj1, Kujtim Lepaja1, Naim Krasniqi2, Agim Zajmi2 1Department of Horticulture, Agriculture University of Tirana, Albania; Department, University of Prishtina, Kosovo. 2Horticultural We investigated the effects of water stress (regulated deficit irrigation - RDI) and mulching on the vegetative growth response of young Williams pear trees. Using a water budget methodology, four levels of irrigation, specifically 100% of evapotranspiration (ET) as control and deficits of 80%, 60% and 40%, were applied to 10 trees during the season, 5 of which were mulched with wood chips at a 20 cm layer. The experiment was conducted in Kosovo during 2013, 2014 and 2015 in a three- to five- year old pear orchard using a nested experimental design. We found significant changes in a series of vegetative growth parameters. Irrigation levels significantly influenced all parameters. Mulching had a positive effect on several physiological parameters. Our results confirmed that a moderate water stress reduces excessive vegetation growth, especially canopy volume, leaf surface and area, LAI and shoot length. It can be concluded that regulated deficit irrigation can be successfully applied to young pears with significant water saving. 193 BOOK OF ABSTRACTS T4-P38 NEW APPROACH OF ELECTROTHERAPY FOR GRAPEVINE VIRUS ELIMINATION Ionela Catalina Guta 1, Elena Cocuta Buciumeanu 1, Lavinia Diana Tataru2, Benedict Oprescu2, Carmen Mihaela Topala 3, 1 INCDBH, Stefanesti-Arges, Romania 2 University of Pitesti, Faculty of Science, Pitesti, Romania 3 University of Pitesti, Faculty of Science , Department of Natural Science, 1 Tg. Vale Str., 110040 Pitesti, Romania The electrotherapy in horizontal electrophoresis tank was applied to remove single and mixed virus infections in grapevine cultivars (Vitis vinifera L.). Electric stimulation of grapevine herbaceous cuttings with electric current of 40-100 mA, for 5-20 min treatment time has been followed by in vitro regeneration of new plants. ELISA negative grapevines have been obtained in different percentages, depending on the virus infection type and experimental parameters. While the Grapevine fleck virus was removed up to 100%, Grapevine fanleaf virus-free plants have been obtained between 44 and 50%, and the lowest virus elimination rate of 25-30% have been registered for Grapevine leafroll associated virus type 1. The complex consisting of Grapevine fleck virus and Grapevine virus A was eliminate in 50% of recovered plants. T4-P39 NON-INVASIVE DETECTION OF RUSSET ON POME FRUIT Michael Blanke 1, Matthias Klemm 1, OLga Roettger 1, Lutz Damerow 2 1 INRES- Horticultural Science , University of Bonn, Auf dem Huegel 6, D-53121 Bonn, Germany 2 Institute of Agricultural Engineering, University of Bonn, Nussallee 5, D-53115 Bonn, Germany Russeting is a fruit quality parameter on pome fruit such as apple and pear. It changes the physical features of the fruit surface; affected fruit peel becomes rough and brown in colour associated with changes in light reflection. The objective of the work was to examine two new innovative technologies as to their suitability for non-destructive determination of russet e.g. for food processing using a) an industrial luster sensor and b) a new type of 3D colour microscope. Russet-free and russet-affected peel sections of pear fruit were examined by three-dimensional colour microscopy at x200 magnification. Russet, i.e. suberinisation of the fruit epidermis, increased peel roughness, defined as the difference between maximum and minimum surface undulations.Russet-devoid peel or smooth-skinned fruit showed a small peel roughness of µm, which was increased in the russeted areas or fruit to µm, i.e. a ca. 2.5 fold increase in surface roughness. Russet peel showed significantly reduced luster, with smaller variation than russet-devoid peel with larger variation. These results indicate that this sensor-based russet detection may also be used on other fruit or possibly for other skin disorders or diseases. T4-P40 OPTIMIZATION OF LIGHT-EMITTING DIODES SPECTRUM FOR GREENHOUSE PLANT LIGHTING BASED ON RELATIVE QUANTUM EFFICIENCY Urbain Niangoran1, LAURENT CANALE1, Feng TIAN1, Theodore Cisse Haba2, Georges Zissis1 1118, RTE DE NARBONNE, 3R3, LAPLACE - UPS, 31062 TOULOUSE cedex9, France; 2BP 1093, Yamoussoukro, Cote d'Ivoire. Greenhouse lighting is the most important parameter for plant growth because it s at root of the photosynthesis process. Light-emitting diode (LED) has many advantages like high efficiency, long lifetime, low voltage, robustness, small size and seems to be one of the best candidate for growth's plants compared with classical sources such as incandescent, high-pressure sodium or fluorescent lamps. One of their main 194 III International Symposium on Horticulture in Europe – SHE 2016 advantage is an easily and convenient product's adaptation that allows us to obtain an ideal growth's plant spectrum with enough intensity. This can be reach thanks to a wide range of LEDs with well chosen wavelength. Thus, in order to enhance photosynthetic and power efficiency, we combined several LEDs to get optimal spectrum for greenhouse plants that induce to minimize the power consumption. To reach these goals, a combination of Gaussian functions was simulated between 320 nm and 780 nm to fit the relative quantum efficiency (RQE) curve. In a second step, according to the bandwidth of the photosynthetically active radiation (PAR) sensor, we optimized spectrum with Matlab software between 400 nm to 700 nm. We propose also a new approach to evaluate photosynthetic efficiency and relative photosynthetic efficacy of LED's wavelength. Results show that the RQE curve could be fit with a great accuracy (RMSE = 3.46%) and the highest photosynthetic efficiency of our basket is obtained with the red LED at 660 nm. T4-P41 PICLÉG': A 'RESEARCH, DEVELOPMENT AND STAKEHOLDERS' ALL-TOGETHER INITIATIVE FOR INTEGRATED PRODUCTION OF FIELD VEGETABLES IN FRANCE Mathilde Causse1, Vincent Faloya2, Benoit Jeannequin3 1Inra- GAFL, BP 94, 84143 Montfavet Cedex, France; UMR IGEPP, BP 35327, 35653 Le Rheu, France; 3)NRA, UE Systèmes de Production en Cultures, Alénya, France 2INRA, Although representing an important activity in Europe with a share of 8.9% of the overall output value of the European agricultural industry, field vegetables are often considered as minor crops and concerned by minor uses, as far as availability of registered pesticides is concerned. Vegetables are recognized for their nutritional value and for their impact on human health. Consumers are encouraged to eat at least 5 portions of fruits or vegetables a day. To meet the expectations of consumers and the society, the production of vegetables has to meet high standards with respect to sustainability and safety. To face those challenges, French field vegetables growers called for an ambitious research program on sustainable cropping systems. Under the umbrella of the Ministry of Agriculture, INRA decided, in 2007, to launch a Scientific Interest Group gathering the growers Union Légumes de France , advisory services CT)FL, Chambres d Agriculture, experimental stations), and stakeholders from marketing (FELCOOP, INTERFEL) to processing (UNILET), called Groupement d )ntérêt Scientifique pour la Production )ntégrée des Cultures légumières G)S P)Clég™ . The purpose of the Group is to identify gaps in knowledge for implementing integrated production strategies, deduct research and development questions to be addressed, build-up common collaborative research and development projects, organize the follow-up of the projects and the promotion of the results towards end-users. So far, 23 projects have been funded, including systemic approach for integrated production and economic analysis in production basins, integrated crop protection against soil-borne pests and diseases at the crop succession scale including biofumigation, insects control through landscape management and chemical ecology, analyzing of trade-offs between fertilization, crop vulnerability and product quality. T4-P42 POWER SUPPLY OF GREENHOUSES BY USING VOLATILE ELECTRICITY GRID WITH HIGH PROPORTION OF RENEWABLES Ingo Schuch, Dennis Dannehl, Uwe Schmidt Humboldt University, Division Biosystems Engineering, Albrecht-Thaer-Weg 3, 14195 Berlin, Germany Currently, about 30% of Germany's annual electricity is generated from renewable sources like wind and the sun. Until 2030, the German government wants to increase this proportion to 50%. Other EU nations have similar plans. Predictive models indicate that this aim will lead to an increase in volatility of the energy supply and will affect electricity pricing. Thus, a temporary oversupply of renewable energy (e.g. at strong wind conditions) can lead to a low price of electricity at the energy exchange market. Otherwise, the 195 BOOK OF ABSTRACTS frequency of annual hours with high pricing will be increased in case of insufficient solar radiation or wind speed. Therefore, flexible capacities are required for energy storage as well as consumption. Related to the power supply of commercial greenhouses, two approaches were considered at Humboldt University: (1) Irrigation water resources could be used as large energy storages for greenhouse air-conditioning in order to obtain the benefits from fluctuations of electricity pricing. (2) The energy for greenhouse air-conditioning will be increasingly used when electricity is cheap. Taking into account a solar thermal collector greenhouse (located in Germany) with electrically-driven heat pump system and heat exchangers for cooling, dehumidification or heating and insulated above-ground water basin for energy storage as well as irrigation, it seems to be possible that by using a volatile electricity grid for greenhouse operation, the consumption of fossil fuels may be reduced more than 90% compared to conventional greenhouses. The feasibility study named ELGEVOS started in 2015 and is supported for a period of two years by funds of the German Government's Special Purpose Fund held at Rentenbank. T4-P43 PRODUCTIVITY OF BABY PLUM TOMATO CV SWEETELLE Peter Newton1, Toni Talab2 1281, Withington Rd, Manchester, M21 0ZA, United Kingdom; Gambetta, 02100 Saint Quentin, France. 241,Boulevard The "Blue print" model for heated glasshouse grown tomatoes in the U.K.was established in the 1960's.In practice it depended on separate thermostats to control day and night temperature and ventilation. With the introduction of climate control computers in the 1980's there was no need fror thermastats,but the same set points were used. There was evidence from records taken from plants in a commercial tomato crop in the 1990's that weight per truss of "round" tomato cv Solairo reduces as the season progresse, i.e. as mean 24 hour temperaturs in the fruit growth period increases, (Acta Hort :-507).It was suggested that the glasshouse temperature mean 24 hour temperature should be as near as possible to 18.5oC. No records have been pulished to supprt this suggestion. In the period 2012 to 2015 inclusive, grower's yield data have bee combined with records of truss and fruit numbers of baby plum cv Sweetelle. A reduction in weight of fruit picked per week from over1 kg/m2 to less than a half of this value was assocoated with a reduction in fruit numbers per truss from over 30 to almost a half of this value with increase in 24 hour mean temperature above 18.5oC in the trusi initiatin period. This supports what was established for cv Solairo;it is suggested that he "Blue print set point model" should be replaced with computer control of mean 24 hour temperature. Experience may show which extremee maximum/ minimum temperatures are undesirable. T4-P44 PROPAGATION OF SCABIOSA HYMETTIA (BOISS & SPRUNER) BY STEM CUTTINGS Maria Papafotiou1, Georgia Vlachou2, A. Akoumianaki-Ioannidou2, Konstantinos F. Bertsouklis2 1Dept. Floriculture & Landscape Architecture, Agricultural University of Athens, 75, Iera Odos, 118 55 Athens, Greece; 2Agricultural University of Athens, Iera Odos 75, 11855 Attiki Athens, Greece; Vegetative propagation of Scabiosa hymettia (Boiss. & Spruner), syn. Lomelosia hymettia (Boiss. & Spruner, Greuter & Burdet), an endemic xyrophyte of Central and Southern Greece, as well as of the Northern Aegean region, was investigated, in order to facilitate the use of the species in urban and suburban areas, and historical Mediterranean landscapes as an ornamental plant. Cuttings from the apical part of shoots, 15 cm long, bearing 2-4 lateral shoots, were collected from wild plants of Attika-Greece, in spring (March), summer (August), autumn (October) and winter (January). The base of the cuttings was immersed either in powder 196 III International Symposium on Horticulture in Europe – SHE 2016 indole butyric acid (IBA) for herbaceous/softwood and hardwood cuttings, or in IBA ethanol-water (1:1, v/v) solutions, at concentration 0 (control), 1000, 2000 and 3000 mg l-1, for two dipping times, 1 or 5 min, and were put for rooting on a mix of 1 peat: 1 perlite (v/v) in a mist system for two weeks and then on a heatedglasshouse bench for further six weeks. Almost all cuttings (92.5-100%) collected in autumn and winter rooted when treated with powder IBA for hardwood cuttings, or with 1000 mg l-1 IBA ethanol-water solution for 1 min, while the controls rooted at 45-65%. In spring and particularly in summer, cuttings rooted at lower percentages compared to autumn and winter (2.5-17.5% rooting of the controls). Best response in spring was achieved with powder IBA for hardwood cuttings (50% rooting), or with 5 min dipping in IBA solutions (50-67.5% rooting). In summer, powder IBA for hardwood cuttings induced the best response (45% rooting), while in IBA solutions rooting ranged from 0-37.5%. T4-P45 PROTOCOL FOR RAPID IN VITRO SHOOT MULTIPLICATION OF ANCIENT APPLE CULTIVARS AND LANDRACES, GROWN IN CZECH REPUBLIC Jiri Sedl|k, Frantisek Paprstein, Ales Matejicek Res. Breeding Inst. of Pomology Holovousy, 50801 Horice, Czech Republic. The apple is the most economically important fruit crop in the temperate climatic zone. In 2012, a project has been initiated to test the resistance of 6 apple landraces and older cultivars grown on the territory of the Czech Republic to fire blight. A decisive part of the project was also testing the applicability of different in vitro culture media and methods of artificial in vitro inoculation of pathogen in save and controllable laboratory conditions. Selected apple cultivars were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. To determine favourable conditions for shoot initiation and development, six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6benzylaminopurine), 0.5 and 1 mgl-1 TDZ (thidiazuron) or 10 mg·l-1 2iP (6- , -dimethylallylamino) purine) were tested. Proliferation rate was defined as the number of newly formed shoots (>10 mm) per initial shoot tip after four weeks of culture. The multiplication rate varied depending on the cultivar and concentration of cytokinins between . and . . The highest proliferation rate was obtained for apple cultivar (vezdnata reneta that produced . in vitro shoots longer than mm on MS medium containing mg L-1 TDZ. On the contrary, the lowest proliferation rate . in our experiments was obtained for cultivar ´Kminova reneta´ on MS medium with 10 mg L-1 2iP. Results obtained in our study confirmed preliminary findings that TDZ was an important plant growth regulator for proliferation and growth in apple micropropagation. Successful in vitro propagation, which can be carried out during the whole year, could provide an alternative method to produce stock material of particular apple cultivars for experiments with artificial inoculation. T4-P46 REDUCED ENERGY USAGE IN COMMERCIAL APPLE STORAGE WITH IMPROVED AIR CIRCULATION Sascha Bottling1, Volker Siegismund1, Marc Spuhler2, Jens W“nsche3, Daniel Alexandre Neuwald4 1(ochschule Biberach, (ochschule f“r , angewandte Wissenschaften, Karlstrasse , Bad W“rttemberg Biberach, Germany; 2Competence Centre for Fruit Growing at Lake, Constance KOB and University of Hohenheim, Physiology of specialty crops, Bad W“rttemberg Ravensburg, Germany; 3University of Hohenheim, Physiology of specialty crops, Emil-Wolff-Str. , Bad W“rttemberg Stuttgart, Germany; 4Physiology of specialty crops, University , of Hohenheim at the Competence Centre for , Fruit Growing - Lake Constance, Ravensburg, Germany Air circulation determines the homogeneity of the climatic conditions in an apple storage room. During one fruit storage season, the effect of changes in fan power on fruit quality parameters and energy demand for ventilation were investigated. Two identical commercial storage rooms (each 48 t) were used for experiments in 2015, one without and one with air deflection and evaporator sealing-off, respectively. Fruit 197 BOOK OF ABSTRACTS temperature probes were placed at 9 fixed positions, air temperature probes at 27 positions and rel. humidity probes at two fixed positions inside each room. The air velocity was measured with a hand held anemometer at 12 positions in each storage room. The fan power needed for ventilation and air circulation was lowered stepwise from 100% to 75%, 50%, 25% and 0% in both rooms, and mean air velocity decreased linearly from 100% to 0%. Air velocity was generally higher in the modified room when compared to the standard room. The modified room was supplied with 50% less fan power than the standard room and with less fan run-time, this resulted in a 40% reduction in energy usage. The fruit temperature probes measured with Pt100, gave similar readings in both roo The results and data from the storage trials will be presented and the potential for energy savings during fruit storage will be discussed. In further experiments, the effects of air turbulence will be determined to allow a comprehensive interpretation of the air velocity measurements. T4-P47 RESEARCH ON FREEZING BEHAVIOR OF SOME CRANBERRY FRUIT GROWN IN ROMANIA Marian Vintila 1, Daniela Veringa 2, Cristian Sorica 3, 1 ICDIMPH - Horting, Intrarea Binelui, 1A, sector 4, Bucharest, Romania 2 No. 1A, Intrarea Binelui Street, District 4, Bucharestr, Romania 3 B-dul Ion Ionescu de la Brad, Nr. 6, Bucharest, Romania Preserving fruits and vegetables by freezing, is one of the most important method of keeping their quality, for a long period of time. Research has pursued various ways of freezing behavior of „Augusta and „Simultan cranberry cultivars. Freezing systems used were a classical freezer, cooler, fast freezing tunnel and an experimental model (equipment) for fast freezing in a nitrogen atmosphere designed by INMA. Experiments were focused on sensory quality, microbiological and nutritional value of frozen fruits and changes in texture, taste and color variation and biochemical components namely vitamins, carbohydrates, etc. In the same time, have been followed and made determination to a number of factors such as freezing time and rate, which have direct influence on the final quality of the frozen product. The results confirmed that cranberry fruits lends itself to preservation by freezing and is recommended a quick freezing process. Simultan variety presented better features compared to „Augusta variety. Research results allow establishing the freezing methods easy to use in Romania rural areas, within family farms whose technical equipment is deficitary. T4-P48 SELECTIVE MECHANICAL THINNING TECHNOLOGY FOR HORTICULTURAL FRUIT CROPS Lutz Damerow1, Michael Blanke2, Claudia Seehuber1 1Institute of Agricultural Engineering, University of Bonn, Nussallee 5, D-53121 Bonn, Germany; University of Bonn, Institut fuer Obstbau, Auf dem Huegel 6, D-53121 Bonn, Germany. 2INRES-Hort.Science, An interdisciplinary concept and novelty in horticulture is presented for selective mechanical thinning of a range of fruit crops. Thinning is a pre-requisite in the majority of fruit crops worldwide in order to overcome or prevent excess crop load in years of alternate bearing (change of years with large and low yields) and to provide regular yields of high quality fruit in terms of size, taste, colour and constituents, as required by the market. The widely-used chemical thinning often uses compounds, commonly referred to as hormones, temperature-dependent and critised by the consumer. Hence, a new device was developed to achieve a wide variety of three-dimensional thinning motions, which consists of three variable horizontal rotors with rotating tines. The objective was to remove individual flowers out of the 5 flowers in a cluster; otherwise the large number of fruitlets within a cluster develops into small, hard, green and unripe fruit. 198 III International Symposium on Horticulture in Europe – SHE 2016 Overall, the success of the selective thinning in apple depended on the interaction between branch angle and singlet portion. Branches facing ° the thinning device were more severely thinned. Selective thinning led to removal of individual flowers out of a flower cluster (rather than removing complete clusters) and increased the portion of the desired singlets from, i.e. flower bunches with one single flower with less fruit to fruit competition for photo-assimilates and subsequently better fruit quality not only in apple, but likely in other fruit such as apricot, peach, nectarine, plum and pear. T4-P49 STUDIES ON ROOTING PERFORMANCES OF CAROB (CERATONIA SILIQUA) CUTTINGS Hamide Gubbuk1, Sadettin Guler2, Recep Balkic1 1Akdeniz University, Faculty of Agriculture, Department of Horticulture, 07058 Antalya, Turkey; Anatolia, Forest Research Institute SAFRI, 07010 Antalya, Turkey 2Southwest Grafting is commonly used vegetative propagation method for carob. Rooting ability of carob cuttings is ambiguous since research results on rooting success of cuttings were quite different. Number of rooting studies on carob cuttings is very limited in Turkey. In order to enhance this knowledge, present study has been conducted on cultivated Kibrisi carob type. Various treatments applied on two types of cuttings derived from either tip (with apical dome) or below tip (without apical dome) of one year old flushes. All the cuttings used in the experiment were one cm in diameter and 20-25 cm in length. Cuttings with apical dome were taken in two different times (April and May) and applied with six different treatments. These were (1) Control - no treatment, (2) 7000 and (3) 9000 ppm indole butyric acid (IBA) treatments, (4) scarification of cuttings, and scarification and either (5) 7000 or (6) 9000 ppm IBA treatments. Cuttings without apical dome were taken in three different times (March, April and May) and applied with 10 different treatments. These were (1) Control - no treatment (2) 7000 and (3) 9000 ppm IBA treatments, (4) 7000 ppm IBA + 100 ppm naphthalene acetic acid (NAA) and (5) 9000 ppm IBA + 100 ppm NAA, (6) scarification of cuttings, scarification and either (7) 7000 or (8) 9000 ppm IBA treatments and finally scarification + 100 ppm NAA and either (9) 7000 or (10) 9000 ppm IBA treatments. Rooting rate, number of primary roots, root length and root diameter were evaluated on rooted cutting. Results of rooting rate as follows; rooting was observed only treatments, 3, 5 and 6 with 10.0 %, 13.3 % and 10.0 % rooting ratio, respectively on cuttings with apical dome, taken in April. The other cutting type (without apical dome) resulted some rooting on treatments 3 and 5 of March cuttings, treatments 2, 5, 6, 8, 9 and 10 of April cuttings and treatments 3, 4, 5, 7, 8, and 10 of May cuttings with rooting rate between 3.3 - 6.7 %. Even though above mentioned treatments yielded with rooted cuttings, the rooting rate was quite low. Considering the scarcity of cutting material production of carob tree and the amount of labor to process it, the results strongly (if not absolutely) suggest that carob production via cuttings with one cm in diameter and 20-25 cm in length is not feasible at least experimented carob type Kibrisi . T4-P50 THE CONTROL OF EPICOMETIS HIRTA PODA IN APPLE ORCHARDS WITH AZADIRACHTIN Slavica Vukovic, Sanja Lazic, Sonja Gvozdenac, Dragana Sunjka University of Novi Sad, Faculty of Agriculture, Trga Dositeja Obradovica 8, 21000 Vojvodina Novi Sad, Serbia; Epicometis hirta damages flowers and flower buds of stone fruits. The chemical control of this pest is not recommended because it occurs in flowering period, when bees and other pollinators are present and can cause their uncontrolled mortality. This experiment aimed to assess the efficacy of biological (azadirachtin) and chemical insecticide (tau-fluvalinate) to E. hirta in apples. Before the application, the content of the active ingredients was determined and physical and chemical properties were tested in accordance with recommendations of FAO and WHO. The experiments were carried out in 2015 in Vojvodina province, Serbia 199 BOOK OF ABSTRACTS sites Kovilj and Čelarevo in apple orchards, using standard OEPP methods and modified method in accordance with the pest biology and apple pheno-fase. Azadirachtin (10 g a.i./l) was applied at 0.3% concentration and tau-fluvalinate (240 g a.i./l) in amount of 0.3 l/ha. Three assessments were made, after one, three and seven days of treatment, by counting the number of dead + paralyzed beetles and determining the damage to apple flowers. The results were presented as efficacy (%). Also a laboratory test was carried out. In Kovilj the efficacy of azadirachtin, depending on the time of evaluation, was 37.5 to 68.6%, and of taufluvalinate . to %. While in Čelarevo the efficacy of azadirachtin was very low . to . % and of tau-fluvalinate ranged from 5.0 to 100%. In both localities, flowers were completely damaged in controls, while the damage was less in treatments with insecticides. In laboratory conditions low mortality was registered after 24 and 48 h of exposure and only after 72 h the mortality was 42.5 to 51.0%, depending on the insecticide. The flower damage was not severe as opposed to control where the entire flowers were eaten. We can conclude that the biological insecticide based on azadirachtin exhibited mainly repellent and partially insecticidal effect on E. hirta in apple orchards. T4-P51 THE EFFECT OF BIOSTIMULATORS TREATMENT UPON SEED BREEDING OF ASTER NOVI-BELGII Mirela Cojocariu, Lucia Draghia, Elena Liliana Chelariu Faculty of Horticulture, USAMV Iasi, 3, Mihail Sadoveanu Alley, Iasi, Romania. Aster novi-belgii is a perennial hemicriptophytes species, commonly grown in the gardens of the Romanian villages. It is a plant valued for its hardiness and vigor, especially for color and abundance of flowering that occurs in early autumn. This paper aims to analyze the influence of different treatments on seed germination regarding the growth and development of Aster novi-belgii seedlings. The experience was conducted in two stages, depending on the state in which the treatments were applied (seeds, respectively seedlings). The first set of experiments (seed germination) was made in four variants in which a control variant (V1) with the non-treated seed and three variants which had immersed seeds in different solutions: distilled water V ; BlackJak solution mL/L V , Sprintene® solution mL/L V . Treatments carried out favored germination and plant emergence and positively influenced the germination percentage, duration and speed of emergence. The best results have been obtained by seed bathing in BlackJak 5 ml/l solution for 1.5 hours. In the second stage of the experience there were used the obtained seedlings, which were picked out after approx. 45 days from sowing, and treatments were applied with the same products used to seeds (BlackJak and Sprintene® , resulting three variants: untreated seedlings V seedlings treated with BlackJak 5mL/L V and seedlings treated with Sprintene® mL/L V . At this stage observations have been made on stems height, number of leaves and number of internodes, the best results being recorded for Sprintene® treated seedlings.The influence of the treatments was highlighted by analysis of the main germination and emergence indicators: the percentage of germination, dynamics of emergence, germination rate, emergence velocity and duration of emergence. 200 III International Symposium on Horticulture in Europe – SHE 2016 T4-P52 THE EFFECT OF MODIFIED ATMOSPHERE PACKAGING ON POSTHARVEST PERFORMANCE OF TWO LOQUAT CULTIVARS Margarita Hadjipieri1, Kalliopi Gavriel2, Georgios Sismanidis2, George Manganaris1 1Anexartisias 33, P.O. Box 50329, 3603 Lemesos, Cyprus; 57, Lemesos, Cyprus 2Anexartisias Loquat (Eriobotrya japonica (Thunb.) Lindl) is the only tree fruit crop in the Mediterranean zone with ripening period during the early spring time, particularly when specific preharvest practices are applied, i.e. cultivation under plastic cover. Such fruit are highly appreciated by the consumers for its taste; thus early harvested fruit enjoy appreciably high prices. However, loquat fruit is characterized by relatively short storage potential, also due to incidence of chilling related disorders. Thus, the purpose of the current study was the evaluation of Xtend® packaging as a means of Modified Atmosphere packaging MAP on postharvest performance of fruits from the main loquat cultivars grown in Cyprus, namely Karantoki and Morphitiki . Fruit were subjected to cold storage at conventional refrigerator °C for weeks and subsequently allowed at room temperature for 0, 2, 4 and 7 days, respectively. For each treatment, 30 fruits were used to determine weight loss, skin colour differentiations (CIEL*, a*, b*), flesh firmness (Texture analyser, Stablemicrosystems , soluble solids content SSC and titratable acidity TA . Morphitiki fruits presented better phenotypic appearance compared to Karantoki fruits after extended storage, mainly due to reduced weight loss. Application of MAP significantly reduced weight loss after removal from cold storage (0.69-0.73% with MAP treatment, 3.52-3.63% with control) for both varieties. However, prolonged retention at room temperature (4 and 7 days) significantly deteriorated the performance of both cultivars. As a technological perspective of the current study, data suggests that Morphitiki fruits that are subjected to MAP can be cold stored up to 3 weeks. Provided that they will be consumed within two days after subsequent maintenance at room temperature. T4-P53 UNRAVEL THE COMPLEXITY OF APPLE AROMA BY DYNAMIC VOCS FINGERPRINTING Brian Farneti1, Iuliia Khomenko2, Mario Di Guardo2, Luca Cappellin2, Francesco Spinelli3, Franco Biasioli2, Fabrizio Costa2, 2Via 1Via capoferro 7b, 47122 forli(FC), Italy; Mach 1, 38010 San Michele allAdige TN, Italy; 3Via Fanin 46, Bologna(40127), Italy The great impact of the aroma on fruit consumer acceptability stimulates the need to step forward in the understanding of this quality trait. In particular, a better and more deep knowledge about the mode of release of volatile organic compound (VOC) is essential. Proton Transfer Reaction Time-of-Fligh Mass Spectrometry (PTR-ToF-MS) resulted a valid alternative to a gas chromatography-mass spectrometry (GSMS) apparatus and a valuable analytical technique to incorporate VOC measurement into plant breeding. Apples were collected from 162 varieties identified within the germplasm collection of the Fondazione Edmund Mach (Trento, Italy). Fruit were harvested from the second half of July till the first half of October based on the optimal commercial ripening stage assessed by external color and IAD (index of absorption difference) changes. The apple aroma profile was investigated, after two months of cold storage, on the headspace produced during the artificial processing of the fruit operated with a device imitating the human consumption. The system, composed by a chewing device coupled with a PTR-ToF-MS, allowed an accurate dynamic VOC fingerprinting suitable to simulate the volatile released during fruit consumption. The obtained results demonstrate the complementarity between the dynamic VOC assessment during mastication and the generally used static headspace analysis. The great advantage of such analytical approach was the possibility to study the kinetics of the volatiles released during eating and the possibility to consider their concentration similar to in vivo condition resulting to an improved characterization of the 201 BOOK OF ABSTRACTS aroma profile. Moreover, differences in textural properties of apple flesh, assessed with a TAXTplus texture analyzer, revealed a possible direct role of the cell wall architectural structure in the regulation of VOC release during consumption. Firm and crispy apple resulted in fact to be characterized by a less intense aroma profile. T4-P54 USE OF BENEFICIAL MICROORGANISMS TO IMPROVE ROOTING SUCCESS RATE IN CHAMAECYPARIS SP. CUTTINGS AND FUSARIUM RESILIENCE IN CYCLAMEN Oscar Stapel1, Maud Tragin2 1ASTREDHOR 2ASTREDHOR STEPP, 52, Rue de St Ilan, 22360 Langueux, France; APL, Centre Floriloire, , rue des Magnolias, Les Ponts de Cé, France; In nature, plants are in constant interaction with soil microorganisms and often plants benefit from their presence to better adapt to their environment. For example, certain soil microorganisms (e.g. AMF) are known to enhance plant nutrition, and facilitate plants in coping with abiotic and biotic stress. In a multipartner project called AQUABIOS, the interest of microorganism use in horticultural substrates was evaluated. One experiment addressed microorganism use in young plant production in plug trays of 2 cultivars of Chamaecyparis. Different commercially available products containing microorganisms (Rhizophagus irregularis or a mix of Glomus sp., Trichoderma harzianum T22, Trichoderma atroviride I1237) were applied separately or in combination. Compost was also mixed in several treatments (15 vol %). The mycorrhizal and rhizospheric fungi were directly incorporated in the soil or applied as drench, at planting of cuttings. The results suggest that microorganisms improve rooting success rate in Chamaecyparis cuttings (between 10 and 30 % of improvement). We also identified two factors that may impact microorganism efficacy: the presence of compost mixed in the substrate and plant genetics. In the 2nd experiment, the same microbial products were evaluated in potted Cyclamen productions with the objective to improve resilience of cyclamen against Fusarium oxysporum fsp. cyclaminis, an important soil-borne disease that is difficult to contain. The results show that commercially available microorganisms tested reduce disease incidence and severity, but only when parasitic disease pressure remains low. The presence of commercially available microbial in horticulture substrates may offer growers confronted with moderate Fusarium attacks an interesting and innovative plant protection strategy. T4-P55 VALORIZATION OF DIGESTATE IN GREENHOUSE TOMATO CROPS Yannick Fleury , Jacques Fuchs , Céline Gilli 1 Agroscope, Route des Eterpys 18, 1964, Conthey, Switzerland 2 Fibl, Ackerstrasse 113, 5070 Frick, Switzerland The digestate is a byproduct rejected during the transformation of waste into biogas. The elimination of this product can be problematic. In Switzerland, biogas industries pay farmers to spread it on their fields. The lack of knowledge on this product may lead to a suboptimal use by farmers. In vegetable crops, the use of digestate is uncommon, even nonexistent for protected crops. The main reasons are the difficulties for application and the risk to contaminate crops. But its low cost and its fertiliser value make it interesting, particularly for organic production where a nitrogen-rich fertilizer readily available and cheap is always sought. In order to assess its potential in greenhouses, a trial was conducted by Agroscope in an organic tomato crop in soil under greenhouse. The digestate was compared to an organic reference fertilizer. The trial was carried out in 2013 in a compartment of a Venlo greenhouse. The application was realized via the drip system. The main objectives of this trial were to estimate the problems that may be encountered for the application with the facilities typically employed in production and to observe the effect on the plants under greenhouse conditions. Plant growth, yield, and fruit quality were monitored during the season. The results obtained show that the digestate allowed a good plant development, a higher yield and a better fruits quality. 202 III International Symposium on Horticulture in Europe – SHE 2016 However, filtration of digestate before application must be improved to avoid risk of clogging the irrigation system. T4-P56 YIELD AND QUALITY OF LETTUCE IN RESPONSE TO THE PLANT POSITION IN PHOTOVOLTAIC GREENHOUSE Antonella Sirigu1, Giulia Roberta Urracci1, Gianluca Carboni1, Francesco Chessa2, Marco Maxia2, Luigi Ledda3 1Agris Sardegna, Agricultural Research Agency, Viale Trieste 111, 09123 Cagliari, Sardinia, Italy; Sardegna, Agricultural Research Agency of Sardinia, Viale Trieste 111, 09123 Cagliari, Sardinia, Italy; 3University of Sassari, Department of Agriculture, Division of Agronomy and Plant genetics, 07100 Sassari, Italy 2Agris In recent years there has been an increasing spread of photovoltaic greenhouses, especially in southern European countries, due to the higher incentives recognized to solar photovoltaic (PV) panels integrated on the greenhouse roofs and/or to local laws which limit the photovoltaic systems on the ground. To maximize the income from the production of electricity, often solar panels cover 50% or more of the roof, but the shading caused by these elements on the growing surface seriously limits the productivity and affects crop development. In order to assess the effects of the spatial distribution of the solar radiation inside these structures on yield and quality of leaf vegetable crops, it was analysed the response of lettuce, grown in two cycles (autumn-winter and winter-spring), in an east-west oriented photovoltaic greenhouse with the 50% of the roof covered by PV modules. The influence of plant position (under plastic or PV roof) and cultivar were analysed as experimental treatments in a split plot design with four replications. Total and marketable yield of the lettuce heads (number and fresh weight) and some quality parameters (dry matter and nitrate content) were evaluated. A significant variability of the total and marketable yield due to the plant position and as consequence to the solar radiation distribution inside the structure during the growing cycle was observed. Furthermore, ranges and variability of the nitrate content of lettuce (expressed as NO3 mg kg-1 of fresh weight) were affected by the plant position and harvest season. In order to maximize yield and quality of the crops, the arrangement of plant rows and transit areas, as well as the management of nutrition, should be optimized in relation to the shading caused by the PV roof during the growing cycle. T4-P57 EFFECT OF INDOLEBUTYRIC ACID (IBA) AND CANE POSITON ON ROOTING OF ROOTSTOCK'S CUTTINGS Katerina Biniari, Ioannis Daskalakis, Despoina Bouza Agricultural University of Athens, Iera Odos 75, Athens, Greece. This study was conducted in order to determine the effect of different concentration of indolebutyric acid (IBA) and cane position on rooting of rootstock s cuttings. Three rootstocks Paulsen, SO , Dog Ridge and grapevine variety Vidiano (Vitis vinifera L.) were examined with five different auxin treatments (control, 60, 120, 250 and 1000 ppm IBA) under hydroponic conditions. A comparative study of the rooting ability was performed between the grapevine variety Vidiano and the three rootstocks, as well as for the part of the cane which roots more easily. The cuttings were collected from the vineyard of the Laboratory of Viticulture of the Agricultural University of Athens. The effect of three factors (variety, part of cane from which the cuttings were taken, IBA concentration) was evaluated concerning the percentage of kalogenesis, the percentage of rizogenesis, the average diameter of roots, the average number of roots per cutting, the total surface of roots and the total length of roots. The results showed that the base part of the cane had the highest average root number for rootstocks 1103 Paulsen (13,42 at 250 ppm), SO4 (10 at 250ppm), Dog Ridge (12 at 120 ppm) and grapevine variety Vidiano (12 at 60 ppm). Regarding the concentrations, those at 120 and 250 ppm showed a high percentage of rooting for rootstocks Dog Ridge (65%, 75% respectively), 1103P (75%, 95% respectively), SO4 (50%, 60% respectively) and for grapevine variety Vidiano (70%, 80% respectively). The treatment of 1000 ppm IBA showed the lowest measurements (kalogenesis, rizogenesis, root number, root length, root surface and average root diameter) at all cuttings of rootstocks SO4 and Dog Ridge and grapevine variety Vidiano. 203 BOOK OF ABSTRACTS Poster Topic – 5 Ornamentals, Landscape And Urban Environment 204 III International Symposium on Horticulture in Europe – SHE 2016 T5-P1 A STUDY ON CUT FLOWER POTENTIAL OF ANTALYA Deniz Hazar1, Nilda Ersoy2, G“lden Tamer1, Ibrahim Baktir3 1Akdeniz University, Agricultural Faculty, Department of Horticulture, 07058 Antalya, Turkey; University, Vocational School of Technical Sciences, 07058 Antalya, Turkey; 3Cyprus International University, Faculty of Agricultural Scie. and Tech, Nicosia, Cyprus 2Akdeniz Antalya Province is situated in south western part of Turkey, along the Mediterranean coast. Economy of Antalya basically based on horticulture and tourism. Cut flower production in Antalya started in early s, and since then the production has been steadily increasing. Nearly 40 % of the Turkish cut flower production is realized in Antalya, and carnation is the number one in the production and it is followed by gerbera, gladiolus, rose and chrysanthemum in order. The biggest share of the flowers has been exported to England, Holland, Germany and Ukraine. Carnation is a leading flower as well in export with 80%. The main problems of the sector in Antalya can be indicated as followed; lack of diversity in number of species and cultivars, the production mostly based on carnation. Breeding studies are not sufficient enough, mother plants are imported in a great extent. Mechanization, harvesting and small size of the production areas are the other problems. The sector needs governmental support especially in transportation costs and subsidies. The production has been doubled year by year even though there are indicated current problems. The biggest advantage of cut flower sector in Antalya are: climate, lower labour cost, plenty of fresh water, an international airport, attitude of growers and their positive approaches to flower culture. The main aim of this study is to crystallize the problems in cut flower production wish has been on agenda for years and also to present the potential of Antalya in cut flower production. T5-P2 ANTI-FEEDING ACTIVITY OF SELECTED BOTANICAL EXTRACTS AND THEIR COMBINATIONS ON PLUTELLA XYLOSTELLA LARVAE Nambe Jababu, Tomas Kopta, Robert Pokluda Mendel University, Valticka 337, Lednice, 691 44 Lednice, Czech Republic Many insect pests including the diamondback moth (Plutella xylostella L.) with a known history of developing resistance to many pesticides, is believed to be the result of continuous and repeated use of pesticides with similar mode of actions and or active ingredients. In nature however, plants would have been extinct; but plants produces chemicals with toxicity to a wide variety of potential herbivores (Bush et al., 1997). Plants under attack releases a cocktail of volatile chemicals that act as cues for these allies, which then attack, consume and reduce the populations of herbivores (De Moraes et al., 1998). The aim of this study was to evaluate and compare the antifeedant and feeding effects (mortality) of Neem, Pyrethrum and Quassia wood chips extracts and their mixtures on DBM larvae. There were 8 Formulations, 5 replications and three concentration series per formulation. Larvae were fed with cabbage leaf discs treated with the various extracts. The area of leaf disks consumed by larvae after 24 hours of exposure was assessed and calculated. Results revealed varying level of mortalities and antifeedant effects among all the formulations. Higher formulation concentration resulted in an increased antifeedant effect and an increased mortality for all treatments. The most effective antifeedant effect was recorded in Pyrethrum, and Neem + Pyrethrum mixture ( recording the least average feeding area of 1.0 % each), followed by Neem + Pyrethrum + Quassia mixture and Pyrethrum + Quassia mixture with 2 % and 3 % feeding area respectively. Pyrethrum, Neem + Pyrethrum and Neem + Pyrethrum + Quassia wood chips extract/extract combinations on the other hand recorded the highest mortality, with 50%, 40% and 60% total mortality respectively. 205 BOOK OF ABSTRACTS T5-P3 CHARACTERIZATION DESCRIPTORS FOR LITHUANIAN LOPHOSPERMUM CULTIVARS Simas Gliozeris1, Alfonsas Tamosiunas2 1Aleksandras Stulginskis University, Studentu 11, Akademija , LT-53067, Lithuania; 2Studentu 11, Akademija, Lithuania. Creeping gloxinia (Lophospermum D. Don) belongs to the cosmopolitan family Plantaginaceae and comes from the Mexican mountains. Many members of this family are highly ornamental and commonly planted in gardens. Several representatives of this family (such as Angelonia, Bacopa, and Antirrhinum...) are now widely grown as ornamental plants. It is relatively rare in Lithuanian gardens. Only a limited number of cultivars are commercially available.A collection of lophospermum s including wild species, was established at the University of Aleksandras Stulginskis, Lithuania. Interspecific hybridization among species of Lophospermum offers the potential for new cultivars and desirable ornamental characteristics. Several interspecific crosses performed between different wild species (L. erubescens D. Don, L. scandens D. Don and L. purpusii (Brandegee) Rothm.) and intergeneric crosses with Rhodochiton atrosanguineus (Zucc.) Rothm. Backcrossing of the F1 hybrids resulted in a number of flower colors enhancing the ornamental value of the genus, new hybrids and cultivars were developed, and descriptor of morphological and decorative traits of were prepared. The observations were performed when the plants were completely developed, at the beginning of flowering. 20 plants or their parts were evaluated. T5-P4 EFFECTS OF GIBBERELLIC ACID APPLICATIONS ON INDUCTION OF PARTHENOCARPY IN BLACK MYRTLE FRUITS Halil Ibrahim Uzun 1, Esra Alim 2, Ibrahim Baktir3 1 Akdeniz Univeristesi, Ziraat Fakultesi, Antalya 07058, Turkey 2 BATEM, Antalya, Turkey 3 Cyprus International University, Nicosia, Cyprus Myrtle (Myrtus communis L.) is an evergreen aromatic and medical bush native to Mediterranean basin. It held an important place in ancient culture. Myrtle oil has been conventially produced from the leaves by traditional distilation methods. Meantime , its fruits are consumed fresh especially by local people or used for liquor production. The common complain by the consumers is the small fruit size and high number of seed in the fruits. The main aim of this study was to decrease seed number and induce partenocarpy(seedlessness) in fruits by 50,100 and 200 ppm Gibberellic Acid(GA3) applications. GA3 applied as spray to the leaves a week before budbreak and also at the beginning of blooming. All the three GA3 applications at budbreak stage had no significant effect on fruit characteristics whereas some GA3 applications at blooming period reduced fruit set in a considerable amount. The seed numbers per berry were recorded 2,8 from 100 ppm GA3 and 4.0 from 200 ppm GA3 applications compared to the control by 10.2 seed. The number of seed per fruit and seed weigth significantly reduced in GA3 treated fruits when compared to the controls. Meantime no significant differences were found among the GA3 dosages. 206 III International Symposium on Horticulture in Europe – SHE 2016 T5-P5 FLOWER COLOR AND FLAVONOID PIGMENTS COMPOSITION OF THE PETALS IN ORNAMENTAL PEACH CULTIVARS OBTAINED BY CROSSING WITH PRUNUS MIRA Larisa Komar-Tyomnaya1,Georgy Zaitsev2 1Nikita, 2Kirova, Yalta, Russian Federation; 31, Yalta, Russian Federation. Ornamental peaches are flowering trees with petals ranging from white and pale pink to purple red color. Prunus mira is often used in breeding programs as a donor of powdery mildew and leaf curl resistance. It also impacts on other traits including flower color. The aim of our work was to study the composition of the flavonoid pigments in flowers of 5 parents and 4 new ornamental peach cultivars, originated from P. mira. The pigments were extracted with acid alcohol from fresh petals and were detected by HPLC analysis. P. mira is characterized by white petals with a pale purple nuance.It was noted that the hybrids from crosses of P. mira and cultivars with purple red and purple pink flowers petals have lighter petal color than the parental cultivars. Hybrids from crosses of P. mira and albiflorum cultivars have a light purple pink flower. Chromatographic analysis demonstrated 12 glycosides cyanides and peonidin in different combinations and also 8 glycosides kaempferol and quercetin in the petal samples. Among colored plants, the highest amount of flavonols (114 mg g-1) and the lowest amount of anthocyanins (0.46 mg g-1) were found in P. mira flowers. )n contrast, in P. persica Manifiс purple red flowers only 16 mg g-1 of anthocyanins and 2.5 mg g-1 of flavonols were detected. much more flavonols (55-88 mg g-1) and a little anthocyanins (1.2-3.4 mg g-1) are accumulated in purple pink flowers of hybrid cultivars 'Lel', 'Mirafic' and 'Zhisele'. This trend is saved in cross with a white flower parent, and that is why Solveig hybrid petals are light pink. The diversity of color shades is probably due to different proportions of cyanides and peonidin glycosides. Thus, the use of P. mira in breeding programs provides the opportunities for creating cultivars with new shades of flower color and higher resistance against fungal diseases. This study was funded by a research grant № -50-00079 of the Russian Science Foundation. T5-P6 HOW CAN INNOVATION OF LOCALLY GROWN FRUIT AND VEGETABLES REDUCE FOOD WASTE IN THE PUBLIC PROCUREMENT PROCESS? Lotta Nordmark1, Madeleine Uggla2, Anna Peterson3, Marie Olsson2 1Department of Biosystem and Technology, 230 53 Alnarp, Sweden; of plant breeding, 291 94 Kristianstad, Sweden; 3Department of Landscape Architecture, Plann, 230 53 Alnarp, Sweden 2Department Quality characteristics at different levels of the supply chain should be linked together better and get higher priority in order to reduce food waste. Public procurement of food in Sweden (food and restaurant services to schools, health and medical care) amounts to 10 billion SEK 2013. The market advertised to the public procurement to be regarded as a growing part of the food sector. Based on European Legislation apply the Public Procurement Process which governs the purchase of public service. In Sweden, the public sector is organized in four levels, government or, provincial, regional and municipal level. The market is characterized by a few larger wholesales, economic associations and a large number of producers of agricultural and horticultural enterprises. Several agricultural organizations provide information to their farming to facilitate the tenders. The National Agency for public procurement given advice to divide tenders into product groups and time, demands on suppliers to manage distribution, several municipalities used coordinated foods distribution. In public procurement there are an increased interest in environmental topics and the requirements of organic food produced and / or locally produced food. Quality criteria for different horticulture products are generally, but not product specific, and not communicated or not documented through the supply chain. The issue here, how to juse an innovative holistic design of the provision and training of quality criteria at various levels such as, sustainable development, taste, variety, nutrition, maturation, postharvest, the refining properties throughout the supply chain and how can these reduce food waste in the public service? Proposal in the supply chain, design and define quality criteria manuals based on innovation in primary production and throughout the supply chain up to the kitchen canteens with feedback at all stages. The methods we use is taken from the well-known supply chain models. 207 BOOK OF ABSTRACTS T5-P7 IDENTIFICATION OF SSR IN GARDENIA JASMINOIDES ELLIS DE NOVO TRANSCRIPTOMIC ANALYSIS Georgios Tsanakas1, Maria Manioudaki2, Athanasios Economou1, Panagiotis Kalaitzis2 1Department 2Department of Horticulture, School of Agriculture, Aristotle University, 54124 Thessaloniki, Greece; of Horticultural Genetics and Biotechnology, Mediterranean Agronomic Institute of Chania, CIHEAM, 73100 Chania, Greece Gardenia jasminoides Ellis is one of the most economically important pot plants for the floral market in Greece. More than 1.5 million pot plants are produced and exported mainly to EU and other countries. A previous study has shown that the Greek gardenia (cv. Pelion) has very low genetic variability and that the cultivated clones can be considered as a commercial cultivar with high genetic uniformity. In order to further analyse this uniformity and to develop SSR molecular markers, total RNA was extracted from gardenia petals and sequenced by using the Illumina HiSeq2000 platform. More than 57.500 unigenes were generated, leading to the identification of 9.549 Simple Sequence Repeats in 7.641 transcripts and in 4.226 unigenes. According to the results, the vast majority of these SSR were monomers, dimmers and trimers, while 1.398 sequences contained more than one SSR. Also, according to the analysis, 3.681 SSR can give amplification products between 100 and 160bp size. Further research is needed in order to evaluate the polymorphism of these markers found and to estimate the PIC values, so that can be applied in SSR studies and HRM (HighResolution Melting) analysis and to evaluate the genetic variation and erosion of the species and the cultivated clones. T5-P8 IMPORTANCE OF GEOGRAPHICAL INDICATIONS (GI) IN FRUIT PRODUCTION, CASE STUDY; ''FINIKE ORANGE'' Lokman ALTINKAYA, Ilhami Tozlu, Recep Balkic, Hamide GUBBUK, Nafiye Adak Akdeniz University, Vocational Higher School of Tech. Sciences, Antalya, Turkey; Geographical Indications (GI)s are quite important for marketing and economy of a given region where GI assigned. It may be used as an efficient tool for socio-economic development of a rural region. Some of the fruit crops i.e., apricot, banana and olive are named after the name of the region where they are grown i.e. Malatya, Anamur and Gemlik, respectively in Turkey. The G)s for these crops were assigned as Malatya Apricot , Anamur Banana and Gemlik Olive and help to further improve these regions economy, culture and tourisms. They simply progressively advance the development of these regions. Turkey is an important citrus exporter and producer. More than 85 % of citrus production takes place in Mediterranean coast of Turkey. Finike, a town located close to the westernmost region of Mediterranean coast, produces the best quality Washington Navel oranges in Turkey. The name Finike Orange is becomes an idiom to express quality. And finally, the town has got a geographical indication G) as Finike Oranges in Turkey. Some of the distinct features of Finike Orange are elucidated by comparing fruits with neighboring town, Kumluca. Kumluca is another citrus producer town20 Kmapart from Finike. Results showed that Finike Oranges were found to be smaller in size (270 vs.300 g) and contain higher Soluble Solid (SS) and lower acid content with better sugar/acid balance. Peel color was also deeper and thinner in Finike Oranges compared to that found oranges grown town of Kumluca. It appears that microclimate of Finike has critical impact on quality of its orange. Long term climatic data suggest that chief climatic factors for this high quality are higher diurnal temperatures . °C Vs. . °C andrainfall (96.4 cmVs.73.2 cm) recorded in Finike compared to that of Kumluca. 208 III International Symposium on Horticulture in Europe – SHE 2016 T5-P9 MORPHOLOGIC VARIABILITY IN PORTUGUESE MENTHA CERVINA ACCESSIONS Orlanda Viamonte Póvoa1, Leandra Rodrigues2, Noémia Farinha3, Ana Maria Monteiro4 1Esola Superior Agraria de Elvas, Quartel do Trem., AP 254, 7350 Elvas, Portugal; de Biotecnologia Agrícola e, Agro-alimentar do Alentejo, R. Pedro Soare, Campus IPBeja ESAB Ap. 6158, 7801-908 Beja Beja, Portugal; 3Edifício do Trem Auto, Avenida 14 de Janeiro, 7350-903 Elvas, Portugal, 7350-903 Elvas Portalegre Elvas, Portugal; 4)nstituto Sup. Agronomia,Tapada da Ajuda, Dep. Ciências e Engenharia de Biossistemas, Proteção de Plantas, -017 Lisbo Lisboa, Portugal. 2CEBAL-Centro M. cervina is traditionally used in Alentejo to flavour fish and fish soups, as well as for its medicinal properties. In Portugal it is a rare species, occurring in river banks and other damp and wet places, which require a longer flooded period that is characteristic of the Natura priority habitat temporary deep ponds pt . This study was based on 30 accessions from backyards and natural habitat collected during 2002-2003 in the Alentejo region. Further collections in natural habitat were achieved in 2005, accounting a total of 17 acessions from natural habitat. From each sample, 15 plants were observed, during 2 years growing seasons. Data from 33 Morphological descriptors were submitted to multivariate Principal Components Analysis (CPA) and cluster analysis. High morphologic variability in the species is confirmed by its high statistical difference between accessions on most of the morphologic descriptors. The initial list of 33 morphologic descriptors was reduced to a proposal list of 14 descriptors, measuring plant architecture features, productivity indicators and also phenology descriptors: Plant height; Biomass; Basal leaf length (cm); Basal leaf width (cm); Inflorescence 1st leaf length (cm); Inflorescence 1st leaf width (cm); Number of days for flowering (date); Main stem length (cm); Main stem diameter – basal; Main stem diameter – below inflorescence; Main stem - number of nodes below inflorescence; Main stem - number of branches below inflorescence; Number of flowers in half of the 1st verticillaster; Number of flowered verticillasters. A list of selected accessions with high crop production potential is also presented. T5-P10 MULTI-SPECIES NEAR INFRARED CALIBRATION MODELS FOR PREDICTING NITROGEN STATUS OF ORNAMENTAL CUTTINGS ' STRATEGIES FOR SCOPE EXTENSION Dieter Lohr1, Peter Tillmann2, Siegfried Zerche3, Uwe Druege3, Elke Meinken1 1Am Staudengarten 14, Freising, Germany; 2Teichstr. 35, Kassel, Germany; 3Kuehnhaeuser Strae 101, Erfurt, Germany Because even a slight nitrogen deficiency reduces rooting capacity of ornamental cuttings, managing an adequate nitrogen status of stock plants is of great importance for growers. However, analysis of potting media or nutrient solution only gives indirect information about nutritional status of stock plants. On the other hand, analysis of nitrogen in plant tissue provides direct information but is time-consuming and expensive. For pelargonium and chrysanthemum cuttings, near infrared spectroscopy (NIRS) has been demonstrated as a valuable tool to determine nitrogen status. A major obstacle for practical application is the quite high number of samples (about 250 per species) needed to build these calibration models. Thus, the aim of the current research was to extend the scope of basic calibration models – including only pelargonium and chrysanthemum cuttings – to New Guinea impatiens, osteospermum and poinsettia with a minimum of samples. If basic calibration models were directly applied to impatiens, osteospermum and poinsettia in most cases an unacceptable high Bias was observed. After addition of about 35 samples per species and recalculation of 209 BOOK OF ABSTRACTS calibration models standard error of prediction (SEP) and Bias for each of the three species were quite good but still higher than those achieved for chrysanthemum and pelargonium. Reducing the number of chrysanthemum and pelargonium samples in the calibration set by selection of most representative samples further improved the prediction performances for the three new species, without impairing these for chrysanthemum and pelargonium. Results showed that near infrared calibration models build for single species can be broadened to multispecies calibration models by adding only few new samples to the calibration set. However, strongly uneven distribution of sample number per species negatively affects prediction performance for under-represented species. T5-P11 NEW PEACH CULTIVARS BY NIKITA BOTANICAL GARDENS WITH HIGH RESISTANCE TO LEAF CURL (TAPHRINA DEFORMANS TUL.) Anatoliy Smykov1, Yulia Ivashchenko2, Olga Fedorova2, Tatyana Shishova2 2Nikita 1Crimea, Yalta, Russian Federation; Botanical Gardens, Yalta, Russian Federation Breeding of cultivars with high resistance to leaf curl is one of the most important tasks of peach breeding in Nikita Botanical Gardens. The horticulture needs the peach cultivars with high resistance to this disease for obtaining stable yields without using pesticides. Each year from 2008 to 2015 we evaluated our new 30 cultivars for resistance to Taphrina deformans Tul. Thanks to long lasting monitoring we could select the cultivars with reduced susceptibility to leaf curl – less than 25% leaves affection. The most interesting among them are dessert cultivars Krymsky Shedevr and Lakomy, which are recommended for industrial cultivation in southern Russia, especially in the Crimea. The cultivar Krymsky Shedevr has been obtained by the method in vitro from the half-grown embryo received by crossing the cultivars Valiant and Favorita Morettini. Fruits ripen from 10 to 20 July. They are medium to large (110-160 g), wide-oval in shape, slightly asymmetric. The fruit is medium fuzzy, with dark red colour covering 50-75% of the fruit surface. The flesh is yellow, fibrous, with a balanced sour-sweet taste. The stone is medium size, clingstone. Content of sugars are 9,7%, titrable acids – , %, vitamin С – 8,7%. The peach cultivar Lakomy has been obtained by crossing the cultivars Redhaven and Kudesnik. From early to medium ripening term (from 20 to 30 July), fruit is wide-oval in shape and medium-sized (110-140 g). The fruit is medium fuzzy, with dark red colour covering to 75% of the fruit surface. The flesh is yellow, fibrous, juicy with a tasty combination of sugar and acid. The stone is medium size, clingstone. Content of sugars are 11,4%, titrable acids – , %, vitamin С – 7,3%. The above mentioned cultivars are characterized by high resistance to leaf curl and by high quality fruits. These advantages make them very suitable for integrated fruit production and for home gardening conditions. Acknowledgement: This study was funded by a research grant № Foundation. -50-00079 of the Russian Science T5-P12 OBSERVATIONS RELATED THE LONG-EARED OWL (ASIO OTUS) FEEDING IN TWO HORTICULTURAL ECOSYSTEMS Cosmin Alex. Mihai, Florin Stanica Bv. Marasti, 59, Bucuresti, Romania. Long-eared owls (Asio otus) are very important in the ecosystem because they control rodent populations that especially, in anthropogenic ecosystems, are very often harmful. In this context, the study of the ecology and ethology of this night raptor species in anthropogenic habitats is an important task. In 2015 it was estimated that in Romania there was a number between 8,000 and 30,000 breeding pairs of Asio otus that can be a real human help in fighting rodents. If in many countries night raptors symbolize good things, in Romania there are many superstitions related to night owls, which are considered to bring misfortune. Of 210 III International Symposium on Horticulture in Europe – SHE 2016 course, these superstitions are unfounded, and the only misfortune is regarding the rodents. The present paper includes observations made in winter agglomerations (roost) of long-eared owl (Asio otus) in two horticultural ecosystems: a periurban private garden in Ilfov County and an urban green space, between apartment buildings in Bucharest. Birds pellets were analyzed in order to determine the pray composition. The long-eared owls feeding was significantly influenced by the horticultural ecosystem. T5-P13 ORNAMENTAL PEACH CULTIVARS TOLERANT TO POWDERY MILDEW AND LEAF CURL DISEASE Larisa Komar-Tyomnaya Nikita, Yalta, Russian Federation; Ornamental peaches bloom in mid-spring and delight their abundant flowering, the variety of forms and colours of the flowers, crown types. However, their distribution in the urban gardening is constrained by sensitivity to fungal pathogens. To resolve this issue in Nikita botanical gardens breeding programs was included the goal to develop cultivars tolerant to main fungal diseases - powdery mildew (Podosphaera spp.) and leaf curl (Taphrina deformans). As a source of resistance to fungal diseases we used the wild peach species: Prunus mira, P. davidiana, P. kansuensis and their hybrids that were crossed with the best ornamental cultivars. As a result of this work new original peach cultivars 'Zhisele', 'Solveig', 'Lel', 'Malenky Prince', 'Belosnezhka', 'Ruthenia' and 'Freze Grant' were obtained. All cultivars are characterized by double or semi-double flowers of white, light pink or intense pink color. They began to bloom in the second half of March - early April with an interval of 22 days between the earliest ('Malenky Prince', 'Belosnezhka') and late ('Lel'). In the collection orchard of peach their damage by powdery mildew estimated from 0 ('Malenky Prince', 'Belosnezhka') to 0.5 marks ('Solveig', 'Lel', 'Ruthenia'). Average damage of cultivars by leaf curl was about 1 mark, except for the 'Belosnezhka' resistant to this decease. In the conditions of urban gardening resistance to fungal pathogens was higher. On the South Coast, these cultivars are not damaged by frost. In the continental conditions of steppe Crimea 'Zhisele' and 'Lel' showed the greatest winter hardiness. T5-P14 PLANT EXTRACT CONSTITUENTS FROM INSECTICIDAL EFFECTS OF CORIANDER, ORIENTAL VALERIAN, CINNAMON AND GALANGAL ON MAJOR PESTS Bueyong Park1, Jun-Ran Kim2, In-hong Jeong1 1Wanju county, RDA, NAS, Crop protection division, Wanju, Korea (Republic of); 2Gunsan, Gunsan, Korea (Republic of) The common pests of horticulture plants, green peach aphid (Myzus persicae Sulzer), two-spotted spider mite (Teyranychus urticae Koch), diamondback moth (Plutella xylostella) and Pochazia shantungensis (Ricania sp.) are becoming major pests in human dwellings in a variety of ways across the world. For management of these pests, coriander (Coriandrum sativum L.), oriental valerian (Valeriana fauriei Briquet), cinnamon (Cinnamomum cassia Blume) and galangal (Alpinia officinarum) plant extracts from four plants were tested and the insecticidal effects were evaluated. The tested plant extacts were prepared by steam distillation, solvent extraction, supercritical extraction method from each plant. Each plant extracts substances were treaten to larvae and nymphs of above pests by leaf dipping method. After 24 hours, each plant extract showed broad insecticidal effects on two-spotted spider mite, diamondback moth and Pochazia shantungens excepting for aphid. 211 BOOK OF ABSTRACTS T5-P15 RADIO FREQUENCY IDENTIFICATION SYSTEM PERFORMANCE IN ORNAMENTAL PLANT OPERATIONS Rodney Thomas Fernandez 1, Ryan Gawronski 1, Lindsay Daschner1, Lynne Sage1, Sam Doane 2, Heather Stoven 3, John Lea-Cox 4, Bruk Belayneh 4 ,Bridget Behe 1 1 Michigan State University, Dept. of Horticulture, East Lansing, MI 48824-1325, United States of America 2 JF Schmidt Son, 9500 SE 327 Ave, Boring OR 97009, United States of America 3 Oregon State University Extension, 2050 Lafayette Ave, McMinnville OR 97128, United States of America 4 Dept of Plant Science and Landscape Arch., 2120 Plant Sciences Building, College Park MD 20742, United States of America Radio frequency identification (RFID) systems have the potential to provide electronic information linked to associated individual plants or plant products as they move through the production chain. Additionally, for mobile crops such as container-grown plants, they have the potential to extend the benefits of precision farming to these crops since crop history can follow the crop as it moves. Performance of different types of RFID labels was evaluated for use on ornamental plants in the US states of Maryland at a cut flower operation; Michigan at a commercial greenhouse, 2 retail garden centers and a research nursery at Michigan State University; and Oregon at a commercial nursery. Three different RFID label types were used: a vinyl slip-on label, a polyvinyl chloride stake label and a vinyl adhesive label. Labels were used during production in the Michigan commercial greenhouse and research nursery and the Oregon nursery and were used postproduction in the Maryland cut flower operation, the Michigan garden centers and the Oregon nursery. The ability to read the different RFID label types with a RFID reader system was evaluated under the various production and post-production environments. Reading effectiveness was related to how and where the labels were attached to the products, the type of product, and the distance between the label and reader system antenna. In general, adhesive labels were least effective when attached to container-grown plants. Slip-on labels and stakes performed best in most environments. As the distance between the label and the reader antenna increased, reading effectiveness decreased. The system was most effective in post-production use where the distance between the reader antenna and the label was least and there was less interference from large numbers of containers between the reader antenna and the labels. Details of performance by type of label and production environment will be presented. T5-P16 RECENT DEVELOPMENTS IN THE APPLE WORLD MARKET: THE REGIONALIZING EFFECT OF THE PHYTOSANITARY BARRIERS? Pasquale Lubello1,Federica DeMaria2 1Supagro, 2, Place Viala, 34000 Montpellier, France; Po 114, 86039 Termoli CB, Italy 2Via The aim of this paper is to analyze the principal changes recorded in the apple world market in the last 25 years. During this period, deep transformations have worked both in the field of production, consumption and, of course, world trade. Based on the available data (FAOSTAT) concerning international trade, we find that global market is increasingly embedded in a logic of regional exchanges. We advance the hypothesis that the opportunities of internationalization of trade, permitted by the economic emergence of some developing countries (especially in Asia), were limited not only by the sudden emergence of dominant local suppliers (ie China for the Asian continent), but also by the development at regional level of similar patterns of phytosanitary regulations. We believe that this regulatory homogeneity, understood as the normative translation of common phytosanitary characteristics for countries belonging to the same geographical area, is an additional factor explaining the construction of regional exchange spaces, and an innovative insight on the role of non-tariff barriers (NTB) in agricultural world trade. Finally, we will try to differentiate natural, trade and institutional commons factors justifying regional regulatory homogeneity, from local factors justifying the existence of some degree of intra-regional variety. 212 III International Symposium on Horticulture in Europe – SHE 2016 T5-P17 STERNBERGIA LUTEA DEVELOPMENT AT DIFFERENT NUTRIENT SOLUTION LEVELS 1 Özg“r Kahraman 1 , Arda Akçal 2 Canakkale Onsekiz Mart University, Faculty of Architecture and Design, Department of Landscape Architecture , 17020 Canakkale, Turkey 2 Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 Canakkale, Turkey This study was conducted to determine the effects of different nutrient solution levels in soilless culture on development of Sternbergia lutea in unheated glasshouse between and , in Çanakkale, Turkey. Sternbergia lutea bulbs with 6-7 cm circumference were used as a plant material and these bulbs were planted into polyethylene bag filled with perlite. The research was set up using randomized plot design with three replications and eight bulbs were planted in each replication. The bulbs were irrigated with six nutrient solution level (0%, 25%, 50%, 75%, 100% and 125%) once a week. Plant weight, leaf number, leaf length, leaf width, leaf thickness, leaf weight, leaf dried weight, root number, root length, root weight, dried root weight, stem diameter, bulb diameter, bulb weight and chlorophyll content were obtained from the research and these parameters were analysed statistically by analysis of variance, multiple comparison test and correlation with SPSS software. Separation of means was by the Duncan s multiple comparison test at p<0.05. As a result of this analysis, different nutrient solution levels have significant effects on plant weight, bulb diameter and root length. Maximum values of plant weight were obtained from 50% (31.816g), 75% (31.497g), 100% (31.203g) and 125% (33.092g), while the lowest plant weight was obtained from 0% (14.992g). The best values of bulb diameter were detected between 35.464 mm and 36.613 mm. The lowest value of root length were archived in 0 % nutrient solution level (5.278 cm), whereas the highest values of root length was archived in 25%, 50%, 75%, 100% and 125%. T5-P18 SYMPTOMS OF CU, ZN, MN AND B TOXICITY IN ORNAMENTAL VRIESEA CULTIVARS Bart Vanhoutte1, Johan Ceusters2, Maurice De Proft1 1Willem de Croylaan 42, 3001 Heverlee, Belgium; 2Kleinhoefstraat 4 , Geel, Belgium In the commercial production of ornamental bromeliads there is very little margin for imperfections on the plants. However, due to their highly efficient nutrient uptake and tendency towards luxury consumption, Bromeliaceae are prone to accumulate various micronutrients to toxic concentrations in their leaves, which may result in leaf damage. Application of Cu, Zn, Mn and B at approximately 10 times the concentrations normally used in commercial Vriesea production did not result in visible leaf damage. Application at approximately 50 times the regular concentrations did result in leaf damage: Cu lead to necrosis at the leaf bases, Zn resulted in a red coloring of the leaf tips while Mn and B had no visible effect on the plants. Analysis of leaf tissue by ICP confirmed that Cu, Zn, Mn and B accumulated to high concentrations in the leaves. When Cu, Zn, Mn and B were applied simultaneously instead of individually this resulted in less leaf damage and lower internal concentrations of each element, indicating competition for uptake. These results suggest that while accumulation of micronutrients occurs in Vriesea, this Bromeliaceae genus is less susceptible to leaf damage due to excessive application of micronutrients compared to for example Guzmania. 213 BOOK OF ABSTRACTS T5-P19 THE BULBLET FORMATION OF GALANTHUS ELWESSI HOOK. IN DIFFERENT IBA AND NAA DOSES Özg“r Kahraman , Arda Akçal2 1 Canakkale Onsekiz Mart University, Faculty of Architecture and Design, Department of Landscape Architecture , 17020 Canakkale, Turkey 2 Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 Canakkale, Turkey This research was carried out to determine the effects of different growth regulators such as IBA and NAA on bulblet formation of Galanthus elwessi (ook. snowdrop in growth chamber at Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture in 2015-2016. The bulbs with 3-4 cm circumference size of Galanthus elwessi Hook. were used as plant material in this study. After snowdrop bulbs were divided into four equal segments, these segments were treated with different plant growth regulators and doses such as 100 ppm IBA, 200 ppm IBA, 400 ppm IBA, 100 ppm NAA, 200 ppm NAA, 400 ppm NAA. 20 segments were mixed with 3 liters of perlite and this mixture was put into black polyethylene bags. The experiment was established according to randomised plot design with 3 replications (bags) and after uprooting bulblets from bags, bulblet formation ratio, bulblet number per segment, bulblet weight, bulblet diameter, bulblet height, bulblet scale number were measured. These parameters were analysed statistically by analysis of variance and correlation test with SPSS 23. Separation of means was by the Duncan s multiple comparison test at p< . . According to analysis, treatments have a significant effect on bulblet weight, bulblet diameter, bulblet height, bulblet formation ratio. The highest bulblet weight was observed in 200 ppm NAA treatment (0.291 g), while the lowest bulblet weight was observed in 100 ppm IBA (0.977 g). The bulblet formation ratio was between 58% and 98.34%. T5-P20 THE EFFECTS OF FERTILIZATION ON THE GROWTH AND FLOWERING OF LILIUM REGALE SPECIES Maria Brinza, LUCIA DRAGHIA, ELENA CHELARIU ALEEA MIHAIL SADOVEANU NR 3 IASI , 700420 IASI, Romania The research aimed to establish the influence of Osmocote®Pro fertilizer on ornamental characteristics, photosynthetic pigments content and superoxide dismutase activity at Lillium regale species. The experience was organized in four variants, each with three repetitions,and each repetition having 10 bulbs. The growth of plants in the four experimental variants was achieved without fertilization in case of the control variant M and under fertilization conditions with Osmocote®Pro for the rest of variants V - 4g fertilizer / plant; V2- 6g fertilizer / plant and V3- 8gfertilizer / plant). The influence of fertilization on the plant growth and development has been assessed by plant height, number of flowers, cups diameter and length. The growth in height and capacity of plant flowering were stimulated by applying the fertilizer, regardless of the dosage used. The application of fertilization with Osmocote at Lilium regale species caused an increase in plant height, number of flowers per plant, cup diameter, the best results being highlighted at the variant at wich was applied to the highest dose of fertilizer. Compared with the control, the increasing of the concentration determined a spore growth regarding the plant height, number of flowers, diameter and length of the cups. At the physiological level, at the fertilized variants was observed an increase in the total content of assimilating pigments, the content of chlorophyll a, b. Fertilization of the plants was positively correlated with enzymatic activity, in the case of fertilized variants was observed an decrease of superoxide dismutase activity compared to the control. 214 III International Symposium on Horticulture in Europe – SHE 2016 T5-P21 THE HYBRIDIZATION OF WHITE FLESH NECTARINE WITH SOME SPECIES AFFECTING POMOLOGICAL PARAMETERS Engin G“r1, Murat Eker 2 1 Anakkale Onsekiz Mart University, Lapseki Vocational School, 17800 90 anakkaleLapseki, Turkey 2 Srnak University Faculty of Agriculture, Department of Horticulture, 73000 350305rnak, Turkey White nectarines are the endemic genotype grown in Canakkale which have the unique characteristics of desirable and rich flavor. White nectarines are the original materials for breeding of new stone fruits varieties that have high quality. In this research, white nectarine types have been hybridized with plum, peach, almond and apricot cultivars in from 2009 to 2011. Pomological characteristics of white nectarine types obtained by hybridizations were determined. According to the results of the study there were significant differences among the hybrid fruits for fruit weight (g), fruit width (mm), fruit length (mm), flesh firmness (), seed weight (g), flesh ratio (%), colors of skin and flesh of the fruits (hue angle, chroma and lightness). T5-P22 THE RESPONSE OF LEUCOJUM AESTIVUM L. TO DIFFERENT METHODS OF VEGETATIVE PROPAGATION ON BULBLET FORMATION AND GROWTH CHARACTERISTICS Arda Akçal , Özg“r KA(RAMAN 2 1 Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 ANAKKALE, Turkey 2 Canakkale Onsekiz Mart University, Faculty of Architecture and Design, Department of Landscape and Architecture, anakkale, Turkey In the present study it was aimed to determine the usage possibilities of different propagation techniques for bulblet formation and development in Leucojum aestivum L. . The research was conducted in growth chamber at Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture in 20152016. The bulbs of Leucojum aestivum L.with a diameter of 6-7 cm were used as a plant material. The trial was settled on randomized plot design with 3 replications, 10 pieces of bulb were used per replication. After the sterilization was completed, 7 different propagation technique were applied on bulbs, such as cross-cut from the bottom, cross-cut from the top, twin scale method, twin scale with semi hub, slicing four, slicing eight and scooping. Samples were include on perlite in PE bags and incubated 12 weeks in a growth chamber (average 20 0C temp. and %75 RH). Results of the study showed that treatment position on the bulb and application method is an important factor for bulblet formation, due to using different methods of vegetative propagation has significant effects on formation and quality of bulblets. T5-P23 WATERCORE SEVERITY OF `FUJI´ APPLES FROM DIFFERENT POSITIONS IN THE TREE CANOPY UNDER DIFFERENT CROP LOADS Daniel Alexandre Neuwald1, Corina Serban2, Roy J. McCormick1, Ines Hanrahan3 1Competence Centre for Fruit Growing at Lake, 6Constance KOB, Ravensburg, Germany, 88213 Baden Wurttemberg Ravensburg, Germany 2Washington State University, Department of, Horticulture, Pullman, WA, Pullman WA, United States of America 3Washington Tree Fruit Research Commission, Yakima WA, United States of America Watercore (WC) is a physiological disorder related to carbohydrate metabolism in apple fruit. Affected fruit develop water-soaked tissue around the vascular bundles and/or fruit core when the intercellular spaces are filled with fluid. WC normally develops as Fuji fruit mature on the tree. While mild symptoms dissipate in storage, severe WC can develop into internal browning with alcoholic off-flavours under controlled atmosphere (CA) conditions. Sun exposed apples are more likely to develop WC than shaded fruit. In 2015, at 215 BOOK OF ABSTRACTS the KOB experimental orchard in Southwest Germany, late-harvest Fuji apples with a high incidence of WC (93%) were harvested in a single pick from three different positions within the tree canopy (top/middle/bottom) and from trees with two different crop loads (high = full crop but not over cropped or low = ~54% thinned at 67 d before harvest). After harvest, the fruit weight, density and severity of WC (scale 0 to 5; 0= no WC to 5=severe) were determined for all apples per tree. Fruit from the top and middle canopy positions showed higher WC when compared with fruit from the bottom. Fruit from low crop load trees showed higher WC compared to high crop load trees. Samples were taken for fruit maturity evaluation and sugar composition (sucrose, fructose, glucose and sorbitol). After harvest leaves were picked from selected canopy positions, the leaf area was determined and related to the crop load, fruit size and WC severity. In a separate storage experiment, late-harvest Fuji fruit with high WC incidence were picked from the tops of trees, randomised into 16 treatment lots, treated or not treated with 1-MCP and placed in CA storage for 18 or d at , or °C with or without an d delay. Results are discussed in relation to Fuji tree, harvest and storage management. T5-P24 WINTER-HARDY SWEET CHERRY CULTIVARS AND FORMS OF NIKITA BOTANICAL GARDEN Ekaterina Tarasova Nikitsky Botanical Garden, Researcher, ulitsa Molodezhnaya, 14, kv. 32, 297513 Novyj Sad, Crimea, Russian Federation. In the Steppe Crimea the formation of archesporial tissue in the anthers of sweet cherry begins in the autumn, when the air temperature declines to 10– °C. Flower buds stay on this stage all winter and have the highest winter hardiness at that time. But a short-term provocative thaw in the winter period can become a reason of significant damage of flower buds. We analyzed the damage of sweet cherry flower buds as a result of critically low-freezing temperatures in January 2015 (– °C . The aim of research was a selection of the winter hardiness sources for use in breeding and advancement in other climatic regions. The study included 29 cultivars mainly breeding of Nikita Botanical Garden and 36 intraspecific hybrid for Among cultivars Drogana Βhyoltaya and Βarya Vostoka had received the least damage no more than 28%). A significant degree of damage (among cultivars average – 64%) could be a result of the warm winter period before the temperature has dropped to a critical value. Among studied hybrids 4 forms had received significantly less damage of flower buds (no more than 21%) compared with control cultivars: %, %, %, % . Forms of early and medium ripening had received the average damage of flower buds at 67–69%, and forms of late ripening – no more than 40 %. 216 Author Index 217 BOOK OF ABSTRACTS Author Index Adak N. ................................................. 126, 163, 208 Ahmad N. .............................................................. 63 Aires A...........................................................100, 104 Akbudak N. ................................................... 62, 117 Akçal A. .................................. 134, 145, 213, 214, 215 Alexopoulos A. ............................................118, 182 Algarra Alarcon A. ............................................ 146 Alle V. ................................................................... 114 Alleri M. ................................................................ 103 Almeida D. ............................................................ 65 Amirov B. ............................................................. 150 Ancu I. ...........................................................120, 121 Arhondakis S. ............................................... 54, 144 Aubert M. ............................................................... 67 Balkıç R. ....................................... 162, 163, 199, 208 Berberich J.......................................................... 179 Bertin N. ............................................................... 149 Bevacqua D. .............................................. 85, 86, 87 Biniari K. .......................................................173, 203 Bizzarri M. ............................................................. 84 Blando F. ............................................................... 49 Blanke M. ......... 89, 123, 177, 182, 189, 193, 194, 198 Blazakis K. ............................................................ 74 Bogoescu M. ...................................................... 130 Botton A. ......................................... 81, 159, 169, 175 Bouhsina Z............................................................ 67 Bouzayen M. ........................................................ 39 Braha S. ............................................................... 168 Bravin E. ........................................................ 60, 187 Brinza M. ......................................................165, 214 Budke C. .............................................................. 122 Bunte F. ................................................................. 98 Burnichi F. ........................................................... 158 Busatto N. ....................................................... 71, 75 Butcaru A. ........................................................... 105 Cakirer G. .............................................................. 64 Caplan I. .............................................................. 166 Cardi T. .................................................................. 92 Causse M. .............................................. 45, 149, 195 Cesoniene L. ....................................... 105, 107, 180 Chatzigianni M. ............................................ 53, 189 Christoph C........................................................... 53 Coban N. ......................................................152, 154 Codron J. ............................................................... 61 Cojocariu M. ....................................................... 200 Costa F. .................................................... 71, 75, 201 Costa G. ........................................................... 40, 75 Cristea V. ............................................................... 78 Dane S. .........................................................120, 130 Dannehl D. ................................ 83, 86, 179, 187, 195 Daubaras R. ........................................ 105, 107, 180 Daum D. ................................................ 122, 126, 192 Decombel A. ....................................................... 125 Djuric G. ............................................................... 149 Draghia L. .................................................... 165, 200 Druege U. .................................................94, 95, 209 Duarte E.................................................. 51, 135, 186 Ebrahimzadeh A. ................................................ 95 Economou A. .............................................. 185, 208 Ekelund L. ............................................................. 61 El-Zein Z. ............................................................. 167 Enjolras G. ............................................................ 67 Ersoy N. ....................................................... 181, 205 Farneti B. .................................... 71, 72, 75, 178, 201 Felczynski K. .............................................. 106, 136 Fernández J................................................ 110, 176 Fernandez R....................................................... 212 Fernqvist F. ........................................................... 61 Fleury Y................................................................ 202 Fortes Neto P. ............................................ 135, 186 Frerichs C. ........................................................... 126 Gavat C. ....................................................... 164, 166 Genard M. ..................................................85, 86, 87 Geoffriau E. ..................................................... 50, 97 Georgiadou E. ............................................. 78, 139 Gianquinto G. ............................................... 97, 136 Gkika H. ............................................................... 110 Gliozeris S. .......................................................... 206 Gorina V............................................................... 186 Gosch C. ...................................................80, 96, 156 Gözlekçi S. .......................................................... 126 Graves W. ............................................................. 85 Grzegorzewska M.............................. 106, 119, 130 Gubbuk H. .............................................. 62, 163, 199 Gür E. ........................................................... 133, 215 Gürcan K. ...................................................... 75, 160 Halbwirth H. .............................................80, 96, 156 Hanrahan I. ................................................. 117, 215 Hasan M. ............................................................. 190 Hassenberg K. ...................................... 60, 113, 124 Hazar D. ............................................................... 205 Heinmaa L. .......................................................... 109 Hernández I. ......................................................... 63 Herppich W. ................................... 60, 113, 123, 124 Herter F. ........................................ 143, 160, 172, 184 Hrotko K. ...................................................... 127, 185 Huddleston P........................................................ 69 İmrak B. .................................................................. 56 Izgu T. ........................................................... 157, 167 Jababu N. ............................................................ 205 218 III International Symposium on Horticulture in Europe – SHE 2016 Jensen M. .............................................................. 90 Jozefczak M. ...................................................... 101 Kafkas N. ...................................... 116, 153, 179, 192 Kafkas S. ............................... 144, 145, 152, 154, 192 Kahraman Ö. ....................................... 170, 213, 214 Kalaitzis P. ................. 54, 74, 139, 144, 167, 180, 208 Kallsen C. ............................................................ 140 Kaniszewski S. ................................... 106, 119, 130 Karipidis C........................................................... 119 Katsoulas N. ...................................................... 180 Kavvadias V. ...................................................... 132 Khokhlov S...................................................107, 168 Kilcheuski A. ......................................................... 71 Kittas C. .................................................... 43, 55, 180 Klemm M. ............................................................ 194 Kosson R. ............................................. 106, 119, 130 Koubouris G. ...................................................... 121 Kowalczyk W...................................................... 136 Kucharska D....................................................... 151 Küden A. ........................................................ 56, 116 Kwon M. ............................................................... 120 La Cara F. ........................................................... 142 Lahaye M. ............................................................. 64 Latsko T. .............................................................. 143 Laugale V. ....................................................120, 130 Lazić S. .........................................................113, 199 Lebeda A. .............................................................. 72 Lee S. ................................................................... 147 Legave J. ............................................................... 87 Lepaja K. ......................................................104, 193 Lepaja L. .......................................................104, 193 Lescourret F. ........................................................ 87 Letschka T. ................................................... 71, 100 Lindén J. .............................................................. 102 Lohr D............................................................178, 209 Lopriore G. .......................................................... 135 Lubello P. ............................................................ 212 Lützenkirchen S. ............................................... 163 Machado R. .................................................102, 184 Manganaris G. ................................ 73, 78, 108, 139 Martin C. ................................................................ 39 Masny A. .............................................................. 151 Massa D. ............................................................... 68 Matzner I. .............................................................. 94 Meinken E. ...................................................178, 209 Meir S. .................................................................... 44 Melendez Martinez A......................................... 76 Mello-Farias P. ........................... 148, 160, 172, 184 Meyerding S. ........................................................ 91 Mihai C. ................................................................ 210 Miranda L. ............................................................. 83 Mitrofanova I. ..................................................... 164 Moale C................................................. 134, 170, 191 Mogren L. ...................................................... 50, 102 Molnar M. ............................................................... 77 Moor U.......................................................... 109, 125 Mulaosmanovic E. ............................................ 102 Müller R.................................................................. 51 Munoz-Concha D. ............................................. 175 Nanos G............................................................... 156 Neuwald D. .......................................... 133, 197, 215 Niangoran U. ...................................................... 194 Nikolic D............................................................... 152 Nordmark L. ........................................................ 207 Nothnagel T. ....................................................... 155 Noutsos C. ............................................................ 47 Ntatsi G. ....................................................53, 55, 115 Nunez-Barrios A. ............................................... 129 Oprita V. ............................................................... 166 Orsini F........................................................... 97, 172 Overbeck V. .................................................. 52, 193 Ovesna J. .............................................................. 80 Oz A. ..................................................................... 108 Papafotiou M. ............................................. 147, 196 Paprstein F. ................................................ 102, 197 Park B. .................................................................. 211 Patakas A. ........................................................... 139 Pedreschi R. ................................................. 63, 141 Pennisi G. ............................................................ 172 Perrakis A. ..................................................... 54, 144 Pesakovic M. ...................................................... 176 Petropoulos S. ................................................... 115 Pezzotti M. ............................................................ 43 Pflanz M. ................................................................ 59 Philosoph-Hadas S. ........................................... 44 Pisanu A. ............................................................. 120 Põldma P. ............................................................ 125 Polverari A. ......................................................... 162 Populin F. .................................................... 159, 175 Póvoa O. ......................................... 94, 140, 171, 209 Prosdocimi Gianquinto G. ........................ 97, 172 Puccinelli M. ....................................................... 177 Ramirez T. ........................................................... 183 Reighard G. ........................................................ 127 Riehle A. .............................................................. 148 Rocksch T. ...................................................... 83, 84 Rombach M. ......................................................... 96 Rouphael Y. .................................................. 68, 141 Ruperti B................................................................ 81 Sakaldaş M. ........................................................ 133 Salis K. ................................................................. 154 Samuoliene G. ...................................... 66, 128, 181 Sánchez C. ......................................................... 106 Savvas C. .............................................................. 73 219 BOOK OF ABSTRACTS Savvas D. ................................................. 53, 55, 189 Schmidt U. .......................... 83, 84, 86, 179, 187, 195 Schmittgen S. ..................................................... 191 Schuch I. ............................................... 179, 187, 195 Sedlak J. .............................................................. 102 Serek M. .............................................................. 161 Seutra Kaba J. ..................................................... 77 Shishkina E......................................................... 103 Sidorova T............................................................. 79 Simsek O. ...................................................... 90, 167 Sirigu A. ............................................................... 203 Sismanidis G. ..................................................... 131 Sitarek M. .....................................................150, 171 Sivakumar D......................................................... 59 Smykov A. ............................................ 143, 155, 210 Sonnante G. ....................................................... 161 Sousa M. ............................................................. 102 Southwick S.......................................................... 89 Spendrup S........................................................... 61 Stammler J............................................................ 91 Stapel O. .............................................................. 202 Stich K. ...................................................... 80, 96, 156 Sturzeanu M. ...............................................120, 121 Suh D.................................................................... 120 Suhl J...................................................................... 86 Sunjka D. ......................................................113, 199 Tabaja N. ............................................................. 144 Talhouni M. ........................................................... 52 Tangolar S. ..................................................132, 188 Tarasova E. ........................................................ 216 Tempesta M. ...................................................... 136 Teodorescu E............................................. 158, 165 Topala C. ............................................................. 194 Tozlu I. ............................................. 62, 126, 163, 208 Tsiupka S. ........................................................... 158 Tzortzakis N.......................................................... 55 Uggla M................................................................ 207 Uzun H. ................................................................ 206 Valero D. ................................................................ 49 Van Ruijven J. ...................................................... 88 Vanhoutte B. ....................................................... 213 Veringa D. ................................................... 128, 198 Vintila M. ...................................................... 128, 198 Vio Michaelis S. ................................................... 54 Virsile A. ......................................................... 66, 181 Vogler U. ................................................................ 56 Vryzas Z............................................................... 129 Vukovic S. ................................................... 113, 199 Wadas W. ............................................................ 116 Wagner I. ............................................................. 153 Winterhagen P. .................................................... 79 Wójcik-Seliga J. ................................................. 171 Wünsche J. ......................................................... 197 Xiloyannis C.......................................................... 45 Yazıcı K................................................... 67, 146, 183 Zanic K. ................................................................ 190 Zeipina S. ............................................................ 101 Zerche S. ..................................................94, 95, 209 Zinkernagel J................................................ 88, 122 Zoth M. ......................................................... 114, 148 Кomar-Tyomnaya L. ................................. 207, 211 220 Table of Contents 221 BOOK OF ABSTRACTS Keynote Speakers ........................................................................................................ 38 WHAT CAN PLANT SCIENCE DO FOR HUMAN HEALTH? ........................................................................................................................ 39 GENETIC DISSECTION OF TOMATO FRUIT QUALITY IN THE GENOME ERA: NEW TOOLS FOR IN DEPTH QTL CHARACTERIZATION ................................................................................................................................................................................................. 45 FRUITBREEDOMICS: AN EUROPEAN PROJECT TO HELP BRIDGING THE GAP BETWEEN BASIC RESEARCH AND BREEDING IN FRUITS. .................................................................................................................................................................................... 42 THE REGULATORY NETWORKS UNDERLYING DEVELOPMENTAL SHIFTS IN FLESHY FRUIT: FROM FRUIT SETTING TO FRUIT RIPENING............................................................................................................................................................................... 39 CLIMATE CHANGE AND THE POTENTIAL MITIGATION ROLE OF FRUIT TREE ORCHARDS................................................. 45 THE GENOMIC APPROACH TO DISSECT GXE INTERACTIONS IN GRAPEVINE ............................................................................ 43 APPLICATION OF FUNCTIONAL GENOMICS IN GLANDULAR TRICHOMES OF CISTUS CRETICUS LAID TO THE EXPLORATION OF LABDANE-TYPE DITERPENES BIOSYNTHESIS ..................................................................................................... 46 IMAGING APPROACHES IN PLANT SCIENCES - APPLICATION POTENTIAL IN HORTICULTURAL SHOOT AND ROOT PHENOTYPING ................................................................................................................................................................................................. 44 THE ONE-HEALTH CONCEPT AND ORGANIC PRODUCTION OF VEGETABLES & FRUITS....................................................... 39 NEW DEVELOPMENTS IN DYNAMIC CONTROLLED ATMOSPHERE STORAGE OF POME FRUIT ........................................ 42 SUSTAINABLE VEGETABLES BY FULL CONTROL OF PLANT PRODUCTION IN GREENHOUSE HORTICULTURE........ 41 RECENT TRENDS IN GREENHOUSE DESIGN AND MICROCLIMATE CONTROL ............................................................................ 43 REGULATION OF SENESCENCE AND ABSCISSION IN ORNAMENTALS BY PLANT HORMONES: HORTICULTURAL USE AND MODE OF ACTION ............................................................................................................................................ 44 NEXT GENERATION DATA ANALYSIS USING FLOWERING PLANTS: CASE STUDY AQUILEGIA SPECIES ....................... 47 STRATEGIC INNOVATION AND RESEARCH AGENDA FOR THE FRUIT SECTOR: THE VIEW OF THE INDUSTRY ........ 46 TEMPERATE FRUIT CROPS AT NEW ERA: FROM PRE-HARVEST TO POST-HARVEST ............................................................. 40 Oral Topic – 1 Human Health, Nutritional Value and Horticulture.............................. 48 T1-O1 METABOLIC CHARACTERIZATION OF THE ANTHOCYANIN-SYNTHESIZING TOMATO GENOTYPE 'SUN BLACKTM' ........................................................................................................................................................................................................................ 49 T1-O2 INNOVATIVE TOOLS TO ENHANCE PLUM BIOACTIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY DURING POSTHARVEST STORAGE ........................................................................................................................................................................................ 49 T1-O3 QUALITY DIFFERENCES BETWEEN ORGANIC AND CONVENTIONAL FIELD GROWN VEGETABLES EXPERIENCES FROM A SWEDISH LONG TERM FIELD TRIAL................................................................................................................ 50 T1-O4 CHANGES IN CAROTENOID METABOLISM IN RESPONSE TO BIOTIC AND ABIOTIC STRESS IN CARROT...................... 50 T1-O5 ANTHOCYANIN ELICITATION FOR BIO-SUSTAINABLE COLOURANT PRODUCTION IN CARROT ...................................... 51 T1-O6 MULCH FILMS BIODEGRADATION RATE IN DIFFERENT TYPE OF SOILS ....................................................................................... 51 T1-O7 EXAMINATION OF ION ACCUMULATION AND SOME PHYSIOLOGICAL CHARACTERISTICS OF GRAFTED EGGPLANTS GROWN UNDER SALINITY CONDITIONS .............................................................................................................................. 52 T1-O8 SAVINGS OF PLANT PROTECTION PRODUCTS BY A HIGH LEVEL OF PRECISION IN ORCHARDS ...................................... 52 222 III International Symposium on Horticulture in Europe – SHE 2016 T1-O9 STRAWBERRY PRODUCTION AND NUTRITIONAL VALUE OF FRUITS IN CHANGING ENVIRONMENTAL CONDITIONS ................................................................................................................................................................................................................... 53 T1-10 IMPACT OF NITROGEN FORM AND SUPPLY LEVEL ON GROWTH, YIELD AND NUTRITIONAL VALUE IN HYDROPONICALLY-GROWN CICHORIUM SPINOSUM L ........................................................................................................................... 53 T1-11 Τ(E ROLE OF A PROLΑL 4 HYDROXYLASES IN TOMATO FRUIT DEVELOPMENT ...................................................................... 54 T1-12 CHARACTERIZATION OF THE POLYPHENOLIC PATTERN IN PRUNUS DOMESTICA VARIETIES WITH DIFFERENT FRUIT SKIN COLOR BY ANALYZING THEIR FRUIT JUICES ........................................................................................... 54 T1-13 DETERMINATION OF NITROGEN CONCENTRATION IN HYDROPONICALLY GROWN SPEARMINT .................................. 55 T1-14 THE USE OF LEGUMES FOR SUSTAINABLE AGRICULTURAL PRODUCTION: EUROLEGUME ................................................ 55 T1-15 INTEGRATED PEST MANAGEMENT OF THE WHITEFLY ALEYRODES PROLETELLA IN VEGETABLE BRASSICA....... 56 T1-16 EFFECTS OF SOME DORMANCY BREAKING AGENTS ON HORMONE CONCENTRATION DURING DORMANCY PERIOD IN FLOWER BUDS OF LAPINS SWEET CHERRY CULTIVAR .................................................................................................. 56 Oral Topic – 2 Quality, Authentication, Traceability & Supply Chains.......................... 58 T2-O1 PHYTOCHEMICAL CONTENTS AND OVERALL QUALITY OF LEAFY LETTUCE VARIETIES (LACTUCA SATIVA L.) GROWN IN A CLOSED HYDROPONIC SYSTEM ........................................................................................................................................ 59 T2-O2 FRUIT QUALITY AND YIELD INFLUENCED BY SITE-SPECIFIC MECHANICAL FLOWER THINNING IN 'GALA' AND 'ELSTAR' ORCHARDS....................................................................................................................................................................................... 59 T2-O3 IMPACT OF LIGHT QUALITY ON ANTHOCYANIN SYNTHESIS IN WHITE ASPARAGUS SPEARS (ASPARAGUS OFFICINALIS L.) ............................................................................................................................................................................................................ 60 T2-O4 LOW RESIDUE APPLE PRODUCTION: HIGHER PRODUCTION RISK AND LOWER PROFIT ..................................................... 60 T2-O5 CHOOSING THE OPPOSITE ' CONSUMER ATTITUDES AND THEIR CHOICE OF A HORTICULTURAL PRODUCT ......... 61 T2-O6 MANAGEMENT OF SANITARY AND PHYTOSANITARY RISKS IN THE APPLE INDUSTRY: THE SUSTAIN'APPLE PROJECT............................................................................................................................................................................................................................ 61 T2-O7 THE EFFECT OF LEONARDITE, VERMICOMPOST, FARMYARD MANURE ON YIELD AND LEAF QUALITY OF GARDEN CRESS (LEPIDIUM SATIVUM L.) ........................................................................................................................................................ 62 T2-O8 REDUCTION OF PRE-HARVEST FRUIT DROPS IN WASHINGTON NAVEL (CITRUS SINENSIS OSB.) .................................. 62 T2-O9 PHYSIOLOGICAL, BIOCHEMICAL AND HISTOLOGICAL RESPONSE TO HIGH TEMPERATURE STRESS AT DIFFERENT STAGES OF DEVELOPMENT IN OKRA ABELMOSCHUS ESCULENTUS L. (MOENCH) ....................................... 63 T2-10 UNDERSTANDING POSTHARVEST STRESSES ON RIPENING SYNCHRONIZATION OF HASS AVOCADOS ....................... 63 223 BOOK OF ABSTRACTS T2-11 THE PROPERTIES OF RICE HULL IN SOILLESS AGRICULTURE TECHNIQUE AND THE EFFECT OF PLANT DEVELOPMENT PARAMETERS IN LETTUCE (LACTUCA SATIVA VAR. CRISPA) CULTIVATION .......................................... 64 T2-12 WATER AND CELL WALL CONTRIBUTIONS TO APPLE TEXTURE ...................................................................................................... 64 T2-13 TOOLS FOR STRATEGIC MANAGEMENT OF POSTHARVEST TECHNOLOGY: AN APPLICATION TO THE PORTUGUESE PEAR INDUSTRY ............................................................................................................................................................................ 65 T2-14 PRE-HARVEST RED LIGHT EMITTING DIODE TREATMENT EFFECTS ON NITRATE REDUCTION IN GREEN VEGETABLES .................................................................................................................................................................................................................. 66 T2-15 EFFECTS OF ACTIVE MODIFIED ATMOSPHERE PACKAGING WITH ARGON AND NITROGEN ON POSTHARVEST QUALITY OF LOQUAT ............................................................................................................................................................... 66 T2-16 SANITARY MANAGEMENT PRACTICES IN THE FRENCH FRUIT PRODUCTION ........................................................................... 67 T2-17 EFFECT OF CONVENTIONAL AND ORGANIK FERTILIZATION ON YIELD AND FRUIT QUALITY OF POMEGRANATE (PUNICA GRANATUM L.CV. HICAZNAR) ....................................................................................................................... 67 T2-18 INCREASED PHOSPHORUS USE EFFICIENCY IN BASIL GROWN HYDROPONICALLY WITH LOW PHOSPHORUS CONCENTRATION AND SALINE WATER .......................................................................................................................................................... 68 T2-19 CONFIGURATION OF BABY LETTUCE QUALITY GROWN IN FLOATING SYSTEM IN RESPONSE TO CULTIVAR, CUT NUMBER AND SALINITY SOURCE ............................................................................................................................................................. 68 T2-20 BRAND AWARENESS INCREASES LIKELINESS TO BUY BRANDED HERB AND VEGETABLE TRANSPLANTS .............. 69 Oral Topic – 3 Genetic Resources, Systems Approaches & Biodiversity ........................ 70 T3-O1 GENOME WIDE ASSOCIATION STUDIES AND WHOLE TRANSCRIPTOMIC SURVEY DECIPHER THE FRUIT TEXTURE REGULATION IN APPLE TOWARDS THE SELECTION OF NOVEL SUPERIOR ACCESSIONS .............................. 71 T3-O2 USE OF MARKER ASSISTED SELECTION METHODS IN TOMATO BREEDING IN BELARUS.................................................... 71 T3-O3 HIGH PERFORMING PHENOMICS OF BERRIES AROMA BY PTR-TOF-MS........................................................................................ 72 T3-O4 STATUS OF RESEARCH, BREEDING AND PROTECTION OF CUCURBITS IN RELATION TO CUCURBIT DOWNY MILDEW: THEIR LIMITS AND PERSPECTIVES .............................................................................................................................................. 72 T3-O5 METABOLIC PROFILING OF SUN-DRIED GRAPES DESTINED FOR THE PRODUCTION OF `COMMANDAR)A´ DESSERT WINE ............................................................................................................................................................................................................. 73 T3-O6 ADVANCED MATHEMATICAL ALGORITHMS TO CHARACTERIZE OLIVE VARIETIES THROUGH MORPHOLOGICAL PARAMETERS ........................................................................................................................................................................ 74 T3-O7 MSAPANALYZER: AN ONLINE TOOL FOR ASSESSING METHYLATION SENSITIVE AMPLIFIED POLYMORPHIC (MSAP) MARKERS BY COMPARISON TO REFERENCE STATES............................................................................................................. 74 T3-O8 SUSSEBTILIBY OF SEO/HARLAYNE TYPE RESISTANT CULTIVARS TO PPV-T ............................................................................. 75 224 III International Symposium on Horticulture in Europe – SHE 2016 T3-O9 A MULTIDISCIPLINARY APPROACH REVEALS NEW ASPECTS OF APPLE SUPERFICIAL SCALD ETIOLOGY AND COLD RESISTANCE MECHANISM IN GRANNY SMITH FRUIT................................................................................................................. 75 T3-10 COST ACTION EUROCAROTEN: EUROPEAN NETWORK TO ADVANCE CAROTENOID RESEARCH AND APPLICATIONS IN AGRO-FOOD AND HEALTH .............................................................................................................................................. 76 T3-11 INVESTIGATING THE CAROTENOID PATHWAY OF LOQUAT FRUIT DURING ON-TREE DEVELOPMENTAL STAGES .............................................................................................................................................................................................................................. 76 T3-12 N2 FIXING POTENTIAL OF GLIRICIDIA (GLIRICIDIA SEPIUM) WHEN INTERCROPPED WITH COCOA (THEOBROMA CACAO L.) ......................................................................................................................................................................................... 77 T3-13 EPIDEMIOLOGY OF THE ESCA RELATED FUNGUS PHAEOMONIELLA CHLAMYDOSPORA .................................................... 77 T3-14 ON-TREE TOCOCHROMANOL BIOSYNTHESIS IN `KORONE)K)´ OL)VE FRUIT: AN ANALYTICAL AND TRANSCRIPTOME APPROACH ............................................................................................................................................................................... 78 T3-15 SSR AND ISSR ANALYSIS OF POPULATIONAL VARIABILITY OF LYCHNIS NIVALIS KIT., ENDEMIC AND VULNERABLE ROMANIAN SPECIES .................................................................................................................................................................... 78 T3-16 DIFFERENT EXPRESSION OF HELICASE GENES IN ARTIFICALLY AND NATURALLY INDUCED MANGO FRUITLET ABSCISSION ............................................................................................................................................................................................. 79 T3-17 BIOTECHNOLOGICAL APPROACH TO CONTROL PPV RESISTANCE IN PLUM ............................................................................... 79 T3-18 GENETIC BASIS AND ENVIRONMENTAL FACTORS PLAY ROLE IN CYSTEINE SULPHOXIDES CONTENT OF GARLIC CULTIVARS .................................................................................................................................................................................................... 80 T3-19 MOLECULAR BACKGROUND OF THE FLAVANONE 4-REDUCTASE ACTIVITY OF DIHYDROFLAVONOL 4REDUCTASES .................................................................................................................................................................................................................. 80 T3-20 ETHYLENE RECEPTORS PROTECT THE EMBRYO OF DEVELOPING APPLE FRUITLETS FROM THE ABSCISSION SIGNAL: AN IMMUNE-LIKE MECHANISM? ........................................................................................................................... 81 Oral Topic – 4 Tools, Technologies, Innovations and Applications............................... 82 T4-O1 IMPLEMENTATION OF PHOTOVOLTAICS ON GREENHOUSES AND SHADING EFFECT TO PLANT GROWTH .............. 83 T4-O2 LONG-TERM PREDICTION OF BIOSIGNALS FROM GREENHOUSE-GROWN TOMATO .............................................................. 83 T4-O3 BEYOND RAIN PROTECTION: FRUITS PLASTIC PROTECTION SHEET AS LIGHT SPECTRUM TRANSMISSION INFLUENCE MEANS" .................................................................................................................................................................................................. 84 T4-O4 DEVELOPMENT OF AN OPTIMAL MODEL-BASED ION-SPECIFIC NUTRIENT CONTROL OF HORTICULTURAL CROPS ................................................................................................................................................................................................................................ 84 T4-O5 PROCESS-BASED SIMULATION MODELS AND PLANT PHENOTYPING............................................................................................. 85 T4-06 PHENOLOGY AND FRUIT SET OF PROMISING NORTH AMERICAN SHRUBS IN THE GENUS DIRCA ................................. 85 225 BOOK OF ABSTRACTS T4-07 INAPRO - INNOVATIVE AQUAPONICS FOR PROFESSIONAL APPLICATION ................................................................................... 86 T4-O8 OPTIMIZATION OF CULTURAL PRACTICES IN A MULTI-OBJECTIVE FRAMEWORK VIA A SIMPLE MECHANISTIC MODEL FOR CARBON ASSIMILATION AND ALLOCATION IN FRUIT TREES .................................................. 86 T4-O9 HISTORICAL PHENOLOGICAL RESPONSES IN APPLE TO CONTRASTING WARMING CONTEXTS MAY CLARIFY FUTURE CRUCIAL RESPONSES IN EUROPE .................................................................................................................................................... 87 T4-10 MODELLING FRUIT PLANT-PEST INTERACTIONS, THEIR CONTROL BY CULTIVATION PRACTICES AND THEIR LINK TO FRUIT QUALITY .......................................................................................................................................................................... 87 T4-11 TEMPERATURE-SUM MODELS FOR IRRIGATION SCHEDULING OF VEGETABLE CROPS IN OPEN FIELD ..................... 88 T4-12 TOWARDS ZERO DISCHARGE IN HYDROPONIC CULTIVATION ........................................................................................................... 88 T4-13 NON-DESTRUCTIVE DETECTION OF THE OPTICAL PROPERTIES OF THE WAX BLOOM OF PLUMS ................................ 89 T4-14 DEVELOPMENT OF SWEET CHERRY (PRUNUS AVIUM L.) AND APRICOT (PRUNUS ARMENICA L.) VARIETIES AND PRODUCTION SYSTEMS IN CALIFORNIA AND EUROPE ................................................................................................................ 89 T4-15 EVALUATION AND COMPARISON OF A NEW TYPE OF TEMPORARY IMMERSION SYSTEM (TIS) BIOREACTORS FOR MYRTLE (MYRTUS COMMUNIS L.) ........................................................................................................................... 90 T4-16 DIGITAL PICTURE ANALYSIS FOR PRECISION SCREENING OF SOUR CHERRY PROGENY FOR NECROTIC LEAF TISSUE CAUSED BY BLUMERIELLA JAAPII ..................................................................................................................................................... 90 T4-17 DIRT-QPCR FOR MOLECULAR BIOLOGICAL DETECTION OF RNA ENCODED VIRUSES ........................................................... 91 T4-18 JOB SATISFACTION AND PREFERENCES REGARDING JOB CHARACTERISTICS OF VOCATIONAL AND MASTER CRAFTSMAN SCHOLARS AND HORTICULTURE STUDENTS IN GERMANY ..................................................................................... 91 T4-19 NEXT GENERATION SEQUENCING TECHNOLOGIES FOR THE DEVELOPMENT OF MOLECULAR MARKERS AND THE ANALYSIS OF GENOME DIVERSITY IN CAPSICUM SPP ........................................................................................................ 92 Oral Topic – 5 Ornamentals, Landscape And Urban Environment ............................... 93 T5-O1 AROMATIC AND MEDICINAL PLANTS VEGETATIVE PROPAGATION USING REDUCED-COST NURSERY FACILITIES....................................................................................................................................................................................................................... 94 T5-O2 SURVIVAL AND ROOTING CAPACITY OF ORNAMENTAL CUTTINGS AS RELATED TO TOTAL NITROGEN, STORAGE CONDITION AND SHIFT IN METABOLIC N POOLS ................................................................................................................ 94 T5-O3 IMPACT OF ETHANOL AND ACETALDEHYDE ON CUT CARNATION (DIANTHUS CARYOPHYLLUS L. CV, YELLOW CANDY).......................................................................................................................................................................................................... 95 T5-O4 EFFICIENT ADVENTITIOUS ROOT FORMATION IN PETUNIA HYBRIDA CUTTINGS: WHEN SIGNALS MEET RESOURCES ..................................................................................................................................................................................................................... 95 T5-O5 INCLUSION IN GERMAN HORTICULTURE ....................................................................................................................................................... 96 226 III International Symposium on Horticulture in Europe – SHE 2016 T5-O6 RE-INVESTIGATING SUBSTRATE SPECIFICITY OF DIHYDROFLAVONOL 4-REDUCTASE WITH RESPECT TO THE B-RING HYDROXYLATION PATTERN OF SUBSTRATES.................................................................................................................. 96 T5-O7 INTERDISCIPLINARY RESEARCH IN URBAN AGRICULTURE................................................................................................................. 97 T5-O8 EDUCATION NEEDS AND RESOURCES FOR URBAN AGRICULTURE ENTREPRENEURSHIP .................................................. 97 T5-O9 THE IMPACT OF THE EU VAT DIRECTIVE FOR FLOWERS AND PLANTS ON THE ORNAMENTAL SUPPLY CHAIN................................................................................................................................................................................................................................. 98 Poster Topic – 1 Human Health, Nutritional Value and Horticulture .......................... 99 T1-P1 A SUPERIOR ANTIOXIDANT BIOMATERIAL FROM TOMATO PEEL WASTES FOR BIOTECHNOLOGICAL APPLICATION: OPTIMIZATION APPROACH BY RESPONSE SURFACE METHODOLOGY ....................................................... 100 T1-P2 ALLERGENIC POTENTIAL OF LOCAL, SCAB RESISTANT, RED FLESHED AND COMMERCIAL APPLE CULTIVARS ................................................................................................................................................................................................................... 100 T1-P3 ALTERNATIVE USE OF FRUIT AND ITS WASTE STREAMS IN PHARMA ....................................................................................... 101 T1-P4 CHANGES OF CHLOROPHYLLS, ANTHOCYANINS AND ANTIRADICAL ACTIVITY IN NETTLE (URTICA DIOCA L.) LEAVES DURING VEGETATION PERIOD ................................................................................................................................................. 101 T1-P5 DISPERSAL OF E. COLI O157:H7 GFP+ IN SPROUTING MODULES ................................................................................................... 102 T1-P6 EFFECTS OF NITROGEN LEVEL ON PURSLANE ANTIOXIDANT ACTIVITY .................................................................................. 102 T1-P7 ELIMINATION OF PRUNE DWARF VIRUS FROM SWEET CHERRY CULTIVARS BY IN VITRO CHEMOTHERAPY ...... 102 T1-P8 FIRST RESULTS ON POMEGRANATE PROCESSING FOR JUICE EXTRACTION ............................................................................ 103 T1-P9 FRUIT SUBTROPICAL PLANTS IN THERAPEUTIC AND PREVENTIVE NUTRITION ................................................................. 103 T1-P10 IMPACT OF PARTIAL ROOTZONE DRYING AND MULCHING ON FRUIT QUALITY AND NUTRIENT CONTENTS AFTER STORAGE OF 'POLKA' RASPBERRIES .............................................................................................................................................. 104 T1-P11 INDUCED PRODUCTION OF INDOL-3-YLMETHYL GLUCOSINOLATES IN HAIRY ROOTS OF CHINESE CABBAGE (BRASSICA RAPA SUBSP. PEKINENSIS): PERSPECTIVES TO ENHANCE THE CONTENT OF BIOACTIVE COMPOUNDS ............................................................................................................................................................................................................... 104 T1-P12 INFLUENCES OF SOIL AMELIORATIVE PLANT SPECIES ON THE ORGANIC EDIBLE ROSE CULTURE ........................... 105 T1-P13 INVESTIGATION OF ANTIMICROBIAL PROPERTIES OF DIFFERENT BERRIES ......................................................................... 105 T1-P14 NUTRITIONAL AND FUNCTIONAL VALUE-ADDED OF BIOFORTIFIED APPLE PEELS ........................................................... 106 T1-P15 NUTRITIVE VALUE OF MARKETABLE HEAD CABBAGE AND WASTE OUTER LEAVES OF BRASSICA OLERACEA L. VAR. CAPITATA SUBVAR. ALBA AS AFFECTED BY NITROGEN FERTILIZATION ........................................ 106 227 BOOK OF ABSTRACTS T1-P16 PECULIARITIES OF BIOLOGICAL ACTIVE SUBSTANCES ACCUMULATION IN PERSIMMON FRUITS .............................. 107 T1-P17 QUALITY, NUTRACEUTICAL CONTENT AND ANTIMICROBIAL PROPERTIES OF EUROPEAN CRANBERRYBUSH (VIBURNUM OPULUS) FRUITS ................................................................................................................................... 107 T1-P18 SEARCHING FOR A SULFUR DIOXIDE REPLACEMENT FOR TABLE GRAPES .............................................................................. 108 T1-P19 SUGAR AND TOTAL PHENOL CONTENTS OF HASS AVACADO........................................................................................................... 108 T1-P20 THE EFFECT OF ORGANIC AND CONVENTIONAL FRUIT PRODUCTION METHOD ON MINERAL COMPOSITION AND SENSORY PROPERTIES OF CLOUDY APPLE JUICE......................................................................................................................... 109 T1-P21 TRADITIONAL GREEK PIES ("PITES"): NUTRITIONAL PROFILE ANALYSIS AND BENCHMARKING .............................. 110 T1-P22 YIELD, QUALITY AND GREENHOUSE GAS EMISSIONS IN ORGANIC AND CONVENTIONAL FAVA BEAN CROPS ..... 110 Poster Topic – 2 ......................................................................................................... 112 T2-P1 ACETAMIPRID IN APPLES, DEGRADATION AND RESIDUES ............................................................................................................... 113 T2-P2 ACETIC ACID IS A PROMISING POSTHARVEST TREATMENT TO IMPROVE HYGIENIC CONDITIONS, RETAIN CHARACTERISTIC QUALITY ATTRIBUTES AND PROLONG MARKETABILITY OF SWEET CHERRIES............................ 113 T2-P3 APPLE CROP-LOAD CONTROL UNDERNEATH HAILNET ...................................................................................................................... 114 T2-P4 ASSESSMENT OF HEAVY METAL (CADMIUM) INDUCED CHANGES IN PHYSIOLOGICAL PROCESSES OF DIFFERENT FIELD CROPS..................................................................................................................................................................................... 114 T2-P5 CHEMICAL COMPOSITION AND QUALITY OF VARIOUS GARLIC (ALLIUM SATIVUM L.) GENOTYPES CULTIVATED IN GREECE....................................................................................................................................................................................... 115 T2-P6 COMPARISON OF YIELD AND SOME FRUIT QUALTY CAHARACTERISTCIS OF SOME FRESH AND FRIGO PLANTS OF STRAWBERRY VARIETIES .......................................................................................................................................................... 116 T2-P7 DIFFERENT PRUNING SYSTEMS IN PLUM (PRUNUS DOMESTICA L.) EFFECTS ON FRUIT YIELD AND QUALITY ........................................................................................................................................................................................................................ 116 T2-P8 EFFECT OF BIOSTIMULANTS USE IN EARLY POTATO PRODUCTION IN POLAND .................................................................. 116 T2-P9 EFFECT OF ELICITOR APPLICATIONS ON THE COLOR AND CHLOROPHYLL COMPOSITION OF LEAF LETTUCE (LACTUCA SATIVA L.) ............................................................................................................................................................................................. 117 T2-P10 EFFECT OF FRUIT MATURITY, FRUIT POSITION AND NETTING ON THE DEVELOPMENT OF SOFT SCALD IN MALUS DOMESTICA 'HONEYCRISP' DURING STORAGE ........................................................................................................................ 117 T2-P11 EFFECT OF NITROGEN AND PHOSPHORUS FERTILIZATION ON GROWTH COMPONENTS, YIELD AND TUBER QUALITY CHARACTERISTICS OF TWO POTATO CULTIVARS GROWN UNDER ORGANIC PRODUCTION SYSTEM .......................................................................................................................................................................................................................... 118 228 III International Symposium on Horticulture in Europe – SHE 2016 T2-P12 EFFECT OF NITROGEN FERTILIZATION ON LYCOPENE CONTENT AND QUALITY TRAITS OF TOMATO (LYCOPERSICON ESCULENTUM L.) FRUITS FOR FRESH MARKET AND PROCESSING .......................................................... 119 T2-P13 EFFECT OF SULFATE ANIONS ON NITRATES ACCUMULATION AND TOTAL KJELDAHL NITROGEN CONTENT IN LETTUCE PLANTS CULTIVATED IN PEAT-PERLITE MIXTURE .................................................................................................... 119 T2-P14 EFFECT OF WOODCHIPS MULCH AND PERFORMANCE OF EIGHT BLACKCURRANT CULTIVARS .................................. 120 T2-P15 EVALUATION OF EMA PACKAGE FOR HALF, EIGHTH AND SLICES FRESH CUT OF `SPINOSO SARDO´ GLOBE ARTICHOKE .................................................................................................................................................................................................................. 120 T2-P16 EVALUATION OF HIGHBUSH BLUEBERRY CLONAL PROGENIES FOR FRUIT QUALITY ....................................................... 120 T2-P17 EVALUATION OF LEGUME-CEREAL SEED MIXTURES FOR ENHANCEMENT OF SOIL FERTILITY, CARBON STORAGE AND BIODIVERSITY IN A LOW-INTENSITY OLIVE ORCHARD ...................................................................................... 121 T2-P18 EVALUATION OF THE POSTHARVEST QUALITY INDICATORS OF SOME STRAWBERRY CVS ........................................... 121 T2-P19 FRUIT QUALITY AND YIELD OF STRAWBERRIES AS AFFECTED BY SOIL AND FOLIAR IODINE FERTILIZATION ......................................................................................................................................................................................................... 122 T2-P20 GROWTH AND ANTHOCYANIN ACCUMULATION OF PURPLE CARROTS IS IMPACTED BY HARVESTING DATE, BUT NOT BY REDUCED NITROGEN SUPPLY................................................................................................................................................ 122 T2-P21 HOW CLIMATE CHANGE AND LACK OF CHILLING MAY AFFECT FRUIT GROWING IN A FUTURE WITH WARMER WINTERS ................................................................................................................................................................................................. 123 T2-P22 IMPACT OF HIGH CO2 ON RIPENING BEHAVIOUR AND DISCOLORATION OF BANANA FRUIT (MUSA X PARADISIACA) ............................................................................................................................................................................................................ 123 T2-P23 IMPACT OF POSTHARVEST CHLORINE DIOXIDE TREATMENT ON MICROBIAL DECAY AND PHYSIOLOGICAL QUALITY OF WHITE ASPARAGUS (ASPARAGUS OFFICINALIS L.).................................................................................................... 124 T2-P24 IMPACT OF PRE- AND POSTHARVEST FACTORS ON STORAGE QUALITY FOR LAMB'S LETTUCE (VALERIANELLA LOCUSTA L.) ........................................................................................................................................................................... 124 AN DECOMBEL1, BERT JACOBS2, JORIS VAN LOMMEL3, ISABEL VANDEVELDE3, BERT VERLINDEN2, PETER BLEYAERT1 .................................................................................................................................................................................................................. 125 T2-P25 INFLUENCE OF NITROGEN AND SULFUR FERTILIZATION ON THE YIELD AND QUALITY OF GARLIC......................... 125 T2-P26 INFLUENCE OF NITROGEN FORM AND CONCENTRATION ON YIELD AND QUALITY OF POT GROWN BASIL ......... 126 T2-P27 INFLUENCE OF SEED PRE-TREATMENTS ON SEED GERMINATION AND EARLY SEEDLING DEVELOPMENTS OF JUJUBE (ZIZIPHUS JUJUBA MILL.) .............................................................................................................................................................. 126 T2-P28 LEAF GAS EXCHANGE CHARACTERISTICS AND LEAF WATER POTENTIAL OF DROUGHT STRESSED LINDEN (TILIA SP.) TREES ..................................................................................................................................................................................................... 127 T2-P29 PRUNUS ROOTSTOCK TESTING FOR PEACH TOLERANCE TO PSEUDOMONAS AND ARMILLARIA DISEASES IN SOUTH CAROLINA .............................................................................................................................................................................................. 127 229 BOOK OF ABSTRACTS T2-P30 RELATIONSHIPS AMONG THE ROOTSTOCK AND MORPHOGENETIC BEHAVIOUR OF APPLE TREE IN ASPECT FOR FRUIT QUALITY ............................................................................................................................................................................................... 128 T2-P31 RESEARCH ON THE INFLUENCE OF CARBON DIOXIDE-RICH ATMOSPHERE OF SHORT TERM STORAGE OF BLUEBERRY ................................................................................................................................................................................................................. 128 T2-P32 RESIDUE ANALYSIS AND DISSIPATION OF THE FUNGICIDE BOSCALID IN TABLE GRAPES, WINE AND DISTILLATES ............................................................................................................................................................................................................... 129 T2-P33 ROOTSTOCKS AND GRAPES CULTIVARS RESPONSE TO AN INCREASING SOIL WATER DEFICIT IN THE NORTHERN PART OF MEXICO............................................................................................................................................................................ 129 T2-P34 STRAWBERRY YIELD AND QUALITY IN INTERCROP WITH LEGUMES ......................................................................................... 130 T2-P35 THE CROP OF GRAFTED PEPPERS AN ALTERNATIVE - CONCERNING THE YIELD AND QUALITY - FOR VEGETABLES GROWERS ....................................................................................................................................................................................... 130 T2-P36 THE EFFECT OF NITROGEN FERTILIZATION AND POSTHARVEST TREATMENT WITH 1-MCP AND ETHYLENE ON DURABILITY OF TOMATO FRUITS................................................................................................................................... 130 T2-P37 THE EFFECT OF TWO BORON COMPOUNDS ON GROWTH AND CHEMICAL COMPOSITION OF TWO FRUIT ROOTSTOCKS .............................................................................................................................................................................................................. 131 T2-P38 THE EFFECT OF ZEOLITE, CHEMICAL FERTILIZER AND COMPOST ON YIELD OF LETTUCE, NUTRIENT COMPOSITION AND SOIL PROPERTIES ......................................................................................................................................................... 132 T2-P39 THE EFFECTS OF DIFFERENT NITROGEN AND POTASSIUM LEVELS ON YIELD AND QUALITY OF TWO EARLY GRAPE CULTIVARS GROWN IN DIFFERENT SOILLESS MEDIA .......................................................................................................... 132 T2-P40 THE EFFECTS OF POSTHARVEST WATER SUBMERGING AND 1-METHYLCYCLOPROPENE TREATMENTS ON THE RIPENING OF `GOLDEN DEL)C)OUS´ APPLES FROM TWO HARVEST DATES ................................................................... 133 T2-P41 THE EFFECTS OF PREHARVEST 1-METHYLCYCLOPROPENE (HARVISTA) TREATMENTS ON HARVEST MATURITY AND SHELF LIFE QUALITY OF `SANTA MAR)A´ PEAR CULTIVAR ........................................................................... 133 T2-P42 THE EFFECTS OF TOPPING APPLICATIONS AT DIFFERENT LEVELS ON YIELD AND QUALITY OF SUMMER SHOOTS FOR YALOVA CEKIRDEKSIZI GRAPE CULTIVAR..................................................................................................................... 134 T2-P43 THE INFLUENCE OF CROWN SHAPES ON YIELD QUANTITY AND QUALITY PARAMETERS .............................................. 134 T2-P44 THE ROLE OF MULCHING MATERIALS ON LACTUCA SATIVA PRODUCTION - BRAZIL CASE STUDY............................ 135 T2-P45 VEGETO-PRODUCTIVE BEHAVIOR AND FRUIT QUALITY OF TWO CULTIVARS OF APRICOT, `ORANGE RUB)S®´ AND 'FARBALΑ®', AS )NFLUENCED BΑ DIFFERENT BIOSTIMULANTS .................................................................... 135 T2-P46 YIELD OF ONION (ALLIUM CEPA L.) GROWN FROM DIRECT SEEDING AND SOME SOIL PROPERTIES AS AFFECTED BY LONG-TERM ORGANIC AND/OR MINERAL FERTILIZATION............................................................................... 136 T2-P47 YIELDS AND NITROGEN UPTAKE OF CAULIFLOWER AS AFFECTED BY N RATES AND INTERCROPPING WITH PEA AND CLOVER ..................................................................................................................................................................................................... 136 230 III International Symposium on Horticulture in Europe – SHE 2016 T2-P48 REDUCING POSTHARVEST LOSSES BY FILM PACKAGING OF AFRICAN NIGHTSHADE (SOLANUM SCABRUM M)LL. )MPROVEMENT OF STORABILITY AND POSTHARVEST QUALITY .................................................................................. 137 Poster Topic – 3 Genetic Resources, Systems Approaches & Biodiversity ................... 138 T3-P1 IDENTIFICATION OF GEOGRAPHICAL ORIGIN OF DRY BEANS USING THE ISOTOPE RATIO ANALYSIS ..................... 139 T3-P2 A COMPARATIVE STUDY AMONG DIFFERENT OLIVE CULTIVARS (OLEA EUROPAEA L.) REVEALS DIFFERENCES IN EXPRESSION LEVELS OF GENES INVOLVED IN FRUIT VITAMIN E BIOSYNTHESIS .......................... 139 T3-P3 A PISTACHIO SELECTION FROM THE UNIVERSITY OF CALIFORNIA BREEDING PROGRAM SHOWS PROMISE AS A COMMERCIAL CULTIVAR FOR EARLIER HARVEST MATURITY ............................................................................................. 140 T3-P4 ADAPTATION OF OREGANO (ORIGANUM VULGARE L.) TO CULTIVATION IN ELVAS REGION, SOUTH PORTUGAL.................................................................................................................................................................................................................... 140 T3-P5 AN INTEGRATIVE APPROACH TO UNDERSTAND POSTHARVEST RIPENING HETEROGENEITY OF HASS AVOCADOS.................................................................................................................................................................................................................... 141 T3-P6 ARTICHOKE TRANSPLANT PRODUCTION: EFFECTS OF NUTRIENT SOLUTION DELIVERY AND LEAF CLIPPING ....................................................................................................................................................................................................................... 141 T3-P7 BIOCHEMICAL CHARACTERIZATION OF LEAVES, BURNS AND SHELLS OF BOUCHE DE BETIZAC CHESTNUT CULTIVAR ..................................................................................................................................................................................................................... 142 T3-P8 BIOLOGICAL AND ECONOMIC CHARACTERISTICS OF NEW PEACH CULTIVARS AND HYBRIDS IN THE STEPPE CRIMEA ........................................................................................................................................................................................................ 143 T3-P9 CHILLING REQUIREMENT OF NINE PEACH CULTIVARS GROWN IN SOUTHERN BRAZIL REGION ................................ 143 T3-P10 CO-EXPRESSION NETWORKS DURING TOMATO FRUIT RIPENING ................................................................................................ 144 T3-P11 CONSTRUCTION OF AN ALMOND GENETIC LINKAGE MAP USING F1 POPULATION GULCAN-2 X LAURANNE BY SSR MARKERS ...................................................................................................................................................................................................... 144 T3-P12 CURRENT STATUS OF REGISTERED POME FRUIT SEEDLINGS IN LEBANON ............................................................................ 144 T3-P13 DETERMINATION THE EFFECTS OF GROWTH REGULATORS ON POLLEN VIABILITY AND GERMINATION LEVEL OF SOME TULIP CULTIVARS ................................................................................................................................................................ 145 T3-P14 DEVELOPING NEW MICROSATELLITE MARKERS IN WALNUT......................................................................................................... 145 T3-P15 DIVERSITY OF CITRUS GERMPLASM IN THE BLACK SEA REGION IN TURKEY......................................................................... 146 T3-P16 DYNAMIC PHENOTYPIC GRAPEVINE (VITIS VINIFERA L.) RESPONSES TO CHILLING AND HEATING STRESS ....... 146 T3-P17 ECTOPIC EXPRESSION OF BRASSICA SERINE-ARGININE PROTEINS, BRA015576 AND BRA018581, INCREASES THERMOTOLERANCE IN ARABIDOPSIS THALIANA...................................................................................................... 147 231 BOOK OF ABSTRACTS T3-P18 EFFECT OF CYTOKININS ON IN VITRO BLASTOGENESIS OF THYMELAEA TARTONRAIRA SSP. TARTONRAIRA (L.) ALL ........................................................................................................................................................................................................................... 147 T3-P19 EFFECT OF DIFFERENT CARBON SOURCES ON IN VITRO PROPAGATION OF YELLOW PASSION FRUIT ................... 148 T3-P20 EFFECT OF MECHANICAL THINNING ON FRUITLET ABSCISSION AND VEGETATIVE GROWTH IN APPLE ............... 148 T3-P21 EFFECT ON WATER DEFICIT ON TOMATO FRUIT QUALITY - GENETIC VARIABILITY OF THE FRUIT RESPONSE..................................................................................................................................................................................................................... 149 T3-P22 EVALUATION OF INDIGENOUS FRUITS AND GRAPES GERMPLASM IN THE REPUBLIC OF SRPSKA ............................. 149 T3-P23 EVALUATION OF ONION (ALLIUM CEPA. L) BREEDING SELECTIONS FOR RESISTANCE TO DOWNY MILDEW (PERONOSPORA DESTRUCTOR) IN KAZAKHSTAN.................................................................................................................................. 150 T3-P24 EVALUATION OF SOME PEACH CULTIVARS IN THE GENOTYPE COLLECTION OF RESEARCH INSTITUTE OF HORTICULTURE IN SKIERNIEWICE, POLAND ............................................................................................................................................ 150 T3-P25 EXTERNAL FRUIT QUALITY AND LEAF DISEASE SUSCEPTIBILITY OF FRAGARIA HYBRIDS FROM INTRAAND INTERSPECIFIC CROSSES ........................................................................................................................................................................... 151 T3-P26 FACTORS INFLUENCING MICROPROPAGATION OF GOOSEBERRY (RIBES GROSSULARIA L) ........................................... 151 T3-P27 FLOWERING AND YIELD POTENTIAL OF PROMISING PEACH HYBRIDS FROM CROSSING COMBINATION FLAMINIA X HALE TARDIVA SPADONI .......................................................................................................................................................... 152 T3-P28 GENETIC RELATIONSHIPS AMONG PISTACIA VERA L. CULTIVARS BASED ON SIMPLE SEQUENCE REPEAT MARKERS ...................................................................................................................................................................................................................... 152 T3-P29 HYBRIDIZATION IN WILD PEAR (PYRUS PYRASTER) FROM VARIOUS REGIONS IN GERMANY AND FROM LUXEMBOURG WITH RESPECT TO PΑRUS × COMMUNIS? ................................................................................................................... 153 T3-P30 IDENTIFICATION OF POMOLOGICAL CHARACTERISTICS OF SOME IMPORTANT APRICOT VARIETIES GROWN IN TURKEY ................................................................................................................................................................................................. 153 T3-P31 IN SILICO POLYMORPHISM ANALYSIS FOR THE DEVELOPMENT OF SIMPLE SEQUENCE REPEAT MARKERS IN PISTACHIO .............................................................................................................................................................................................................. 154 T3-P32 IN VITRO PROPAGATION OF CITRUS ROOTSTOCKS AND THEIR GENETIC RELATIONSHIPS ........................................... 154 T3-P33 INHERITANCE OF RIPENING TERMS, YIELD AND RESISTANCE TO FUNGAL DISEASES AT THE PEACH .................... 155 T3-P34 INTROGRESSION OF AGRICULTURAL TRAITS AND RESISTANCE FROM ASPARAGUS WILD RELATIVES INTO THE GARDEN ASPARAGUS (ASPARAGUS OFFICINALIS L.) .................................................................................................................. 155 T3-P35 INVESTIGATIONS ON THE FORMATION OF DIHYDROCHALCONES IN THE MODEL PLANT APPLE (MALUS SP.) .................................................................................................................................................................................................................................... 156 T3-P36 'LEMONATO' PEACH: A SERIES OF CULTIVATED CLONES WITH HIGH FRUIT QUALITY .................................................... 156 T3-P37 MICROPROPOGATION OF SIDERITIS PISIDICA BOISS. ET HEL APUD BENTHAM .................................................................... 157 232 III International Symposium on Horticulture in Europe – SHE 2016 T3-P38 MOLECULAR CHARACTERIZATION OF CYST FORMING NEMATODES DETECTED ON CHINESE CABBAGE IN GANGWON, KOREA .................................................................................................................................................................................................. 157 T3-P39 NEW CULTIVARS OF HIGH QUALITY NECTARINES AT THE CRIMEA ............................................................................................ 158 T3-P40 NEW DWARF BEAN VARIETIES OBTAINED IN ROMANIA ................................................................................................................... 158 T3-P41 OLD IS BETTER: A MULTIPLE APPROACH TO IDENTIFY HYPOALLERGENIC APPLE GENOTYPES ................................. 159 T3-P42 OMIC APPROACHES TO SHED LIGHT ON APPLE FRUIT DEVELOPMENT..................................................................................... 159 T3-P43 PHENOLOGICAL CHARACTERIZATION AND WATER CONTENT OF 'HOUSUI' PEAR TREES UNDER MILD WINTER CONDITIONS ............................................................................................................................................................................................ 160 T3-P44 POLYMORPHIC EXPRESSED SEQUENCE TAG-SIMPLE SEQUENCE REPEAT (EST-SSR) MARKERS IN APRICOT ...... 160 T3-P45 PROTEOLYTIC ACTIVITY, IDENTIFICATION AND EXPRESSION PROFILE OF A KDEL-CYSEP GENE IN COMMON LILAC FLOWERS DEPENDING ON THE FLOWERING CONDITIONS ........................................................................... 161 T3-P46 REGULATION OF FLAVONOID BIOSYNTHESIS IN ARTICHOKE: ISOLATION AND CHARACTERIZATION OF A MYB TRANSCRIPTION FACTOR ......................................................................................................................................................................... 161 T3-P47 RNASEQ ANALYSIS OF GRAPEVINE AND BOTRYTIS CINEREA GENE EXPRESSION DURING LATENT INFECTION OF BERRIES ("NOBLE ROT")...................................................................................................................................................... 162 T3-P48 SEED GERMINATION AND SEEDLING SURVIVAL OF CAROB (CERATONIA SILIQUA) UNDER NATURAL CONDITIONS ................................................................................................................................................................................................................ 162 T3-P49 SEED GERMINATION STUDIES ON GUAVA (PSIDIUM GUAJAVA) ..................................................................................................... 163 T3-P50 SPECIFIC STRESS-RESPONSES OF MALUS UNDER REPLANT CONDITIONS AS AN INDICATOR FOR THE DEFINITION OF RESISTANT/TOLERANT APPLE ROOTSTOCKS ....................................................................................................... 163 T3-P51 STRUCTURAL AND FUNCTIONAL FEATURES OF LEAVES IN SOME FICUS CARICA L. CULTIVARS IN SITU AND IN VITRO ........................................................................................................................................................................................................................ 164 T3-P52 STUDIES REGARDING THE NEW PEACH ASSORTMENTS IN SOUTH-EASTERN ROMANIA................................................. 164 T3-P53 STUDY CONCERNING THE MAIN CHARACTERISTICS OF CLIMBING BEAN SEEDS BELONGING V.R.D.S., ROMANIA GERMPLASM COLLECTION ........................................................................................................................................................... 165 T3-P54 THE BEHAVIOUR OF LOCAL POPULATIONS OF ASTER NOVAE ANGLIAE L. IN DIFFERENT GROWING CONDITIONS ................................................................................................................................................................................................................ 165 T3-P55 THE BEHAVIOUR OF SOME APRICOTS VARIETIES WITH LATE BLOOMING IN SOUTH-EASTERN ROMANIA .......... 166 T3-P56 THE BEHAVIOUR OF SOME NEW CHERRY TREE CULTIVARS DURING THE FIRST YEARS AFTER PLANTING ......... 166 T3-P57 THE EFFECT OF ARABIDOPSIS P4H3 ON GLYCOPROTEIN EXPRESSION AND THE FERMENTATION PATHWAY IN RESPONSE TO HYPOXIA .......................................................................................................................................................... 167 233 BOOK OF ABSTRACTS T3-P58 THE EXPRESSION OF ETR1 AND CTR1 GENES IN 1-MCP TREATED SPRAY CARNATION ................................................... 167 T3-P59 THE IDENTIFICATION OF WALNUT CULTIVARS FROM NIKITA BOTANICAL GARDENS BY SSR-MARKERS ............. 168 T3-P60 THE INFLUENCE OF THE ROOTING SUBSTRATE AND GROWTH REGULATORS IBA AND NAA IN THE NUMBER AND LENGTH OF ADVENTIVE ROOTS OF HARDWOOD CUTTINGS IN BLUEBERRY CV. BLUECROP (VACCINIUM CORYMBOSUM L.) ........................................................................................................................................................................ 168 T3-P61 THE NEW WALNUT BREEDING PROGRAM IN TURKEY ........................................................................................................................ 168 T3-P62 THE PEACH HECATE3-LIKE GENE FLESHY PLAYS A DOUBLE ROLE DURING FRUIT DEVELOPMENT......................... 169 T3-P63 THE RESISTANCE OF APRICOT GENOTYPES TO THE ATTACK OF CERTAIN PATHOGENS IN ROMANIA .................... 170 T3-P64 USAGE OF SEWAGE SLUDGE IN BRASSICA OLERACEA ACEPHALA CULTIVATION ................................................................ 170 T3-P65 VALUABLE BLACKBERRY CULTIVARS TESTED IN RUBUS COLLECTION AT THE RESEARCH INSTITUTE OF HORTICULTURE IN SKIERNIEWICE, POLAND ............................................................................................................................................ 171 T3-P66 VARIABILITY ON MORPHOLOGIC DESCRIPTORS OF PORTUGUESE M. PULEGIUM ACCESSIONS ................................... 171 T3-P67 WATER DYNAMICS AND PHENOLOGY OF 'EVA' APPLE TREES: COMPARISON OF DIFFERENT PLANT PORTIONS UNDER MILD WINTER CONDITIONS IN SOUTHERN BRAZIL ..................................................................................... 172 T3-P68 WATER USE EFFICIENCY OF HYBRID VS OPEN POLLINATED TOMATO (LYCOPERSICON ESCULENTUM) GENOTYPES ................................................................................................................................................................................................................. 172 T3-P69 EFFECT OF VARIOUS CULTURE MEDIA ON IN VITRO PROPAGATION OF GRAPEVINE VARIETIES GIOUROUKIKO AND SERIFIOTIKO (VITIS VINIFERA L.) ....................................................................................................................... 173 Poster Topic – 4 Tools, Technologies, Innovations and Applications ......................... 174 T4-P1 A DEMONSTRATIVE MODULE TO PROCESS CHILI PEPPER PRODUCTS BY FAMILY FARMERS IN CHILE ................... 175 T4-P2 A NEW STRATEGY TO STUDY INTRA-FRUIT RIPENING PROGRESSION IN APPLE (MALUS DOMESTICA L. BORKH) .......................................................................................................................................................................................................................... 175 T4-P3 AGRO-INDUSTRY COMPOSTS AS GROWING MEDIUM IN A FLOATING SYSTEM FOR GROWING BABY-LEAF LETTUCES. ADDED-VALUE TO SUPPRESS PYTHIUM IRREGULARE................................................................................................ 176 T4-P4 BENEFICIAL ROLE OF BIOFERTILIZATION ON YIELD RELATED CHARACTERISTICS OF TWO APPLE CULTIVARS AND SOIL MICROORGANISMS UNDER ORCHARD CONDITIONS ............................................................................. 176 T4-P5 BIOFORTIFICATION OF OCIMUM BASILICUM L. PLANTS WITH SELENIUM .............................................................................. 177 T4-P6 CHANGES IN SURFACE FEATURES OF POMEGRANATE FRUIT WITH ON-INVASIVE SENSOR TECHNOLOGY........... 177 T4-P7 CHARACTERIZATION OF BLUEBERRY AROMA BY NOVEL FAST-GC COUPLED WITH PTR-TOF-MS ............................. 178 234 III International Symposium on Horticulture in Europe – SHE 2016 T4-P8 CHARACTERIZATION OF NITROGEN AND CARBON POOLS OF ORGANIC FERTILIZERS TO PREDICT THEIR NITROGEN RELEASE ............................................................................................................................................................................................... 178 T4-P9 COMMISSIONING AND OPTIMIZATION OF AN INNOVATIVE SYSTEM FOR ELECTROLYTIC WATER DISINFECTION IN GREENHOUSES.................................................................................................................................................................... 179 T4-P10 COMPARISON OF STRAWBERRY (F.XANANSSA CV. FLORIDA FORTUNA) VOLATILES USING VARIOUS SPME FIBRES BY GC/MS TECHNIQUES ....................................................................................................................................................................... 179 T4-P11 DETECTION OF SALINITY STRESS IN GREENHOUSE TOMATO BASED ON CROP REFLECTANCE ................................... 180 T4-P12 DETECTION OF VOLATILE COMPOUNDS IN ACTINIDIA L. BERRIES BY GC-MS METHOD ................................................... 180 T4-P13 DETERMINATION OF PESTICIDE RESIDUES IN SWEET FENNEL SEED (FOENICULUM VULGARE MILL.) BY LC-MS/MS AND GC-MS CHROMATOGRAPHIES ......................................................................................................................................... 181 T4-P14 DYNAMIC VS CONSTANT BLUE:RED LED LIGHT RATIO: EFFECTS ON LETTUCE GROWTH AND NUTRITIONAL QUALITY ........................................................................................................................................................................................................................ 181 T4-P15 EARLY YIELD PREDICTION IN APPLE ORCHARD ..................................................................................................................................... 182 T4-P16 EFFECT OF SURFACE COATING WITH WAXES ON THE DORMANCY OF POTATO TUBERS GROWN FROM TRUE POTATO SEED (TPS) .................................................................................................................................................................................. 182 T4-P17 EFFECTS OF ELECTRIC POWER SUPPLY FOR ARTIFICIAL ILLUMINATION OF GREENHOUSE CROPS AT VOLATILE CURRENT SUPPLY ............................................................................................................................................................................. 183 T4-P18 EFFECTS OF KAOLIN AND SHADING TREATMENTS ON FRUIT DEVELOPMENT AND COLOURATION OF POMEGRANATE (PUNICA GRANATUM L.CV. HICAZNAR) .................................................................................................................... 183 T4-P19 EFFECTS OF LOW CHILL ACCUMULATION ON DORMANCY DYNAMICS OF DIFFERENT APPLE TREES CULTIVARS ................................................................................................................................................................................................................... 184 T4-P20 EFFECTS OF PLANT DENSITY AND THE NUMBER OF EMITTERS ON PLANT GROWTH AND NITRATE CONCENTRATION IN SPINACH CULTIVATED IN SUBSTRATE ........................................................................................................... 184 T4-P21 EFFICIENT VEGETATIVE PROPAGATION OF VARIOUS CLONES OF MASTIC TREE (PISTACIA LENTISCUS VAR. CHIA) THROUGH ROOTING OF SHOOT CUTTINGS................................................................................................................................... 185 T4-P22 ESTIMATED CO2 SEQUESTRATION CAPACITY OF MAPLE (ACER SP.) AND LINDEN (TILIA SP.) TREES..................... 185 T4-P23 EVALUATION OF THE BIODEGRADATION RATE OF MULCH FILMS ............................................................................................... 186 T4-P24 FLUORESCENCE INTENSITY IN APRICOT LEAVES UNDER THEIR DEHYDRATION ................................................................ 186 T4-P25 HOW TO EVALUATE DAMAGES CAUSED BY THE SPOTTED WING DROSOPHILA ON NATIONAL LEVEL? ................. 187 T4-P26 HYPOCHLORITE APPLICATION IN HYDROPONICS WITH ALL ITS BENEFITS AND HAZARDS FOR TOMATO PRODUCTION .............................................................................................................................................................................................................. 187 235 BOOK OF ABSTRACTS T4-P27 IMPACT OF BUDLOAD AND IRRIGATION MANAGAMENT ON YIELD, BERRY QUALITY AND PHENOLIC COMPOSITION OF CV. ALPHONSE LAVALLEE ............................................................................................................................................ 188 T4-P28 IMPACT OF NITROGEN FORM AND SUPPLY LEVEL ON GROWTH, YIELD AND NUTRITIONAL VALUE IN HYDROPONICALLY-GROWN STAMNAGATHI (CICHORIUM SPINOSUM L.) ................................................................................. 189 T4-P29 IMPROVING COLOUR FORMATION IN HORTICULTURE FRUIT CROPS ......................................................................................... 189 T4-P30 IN VITRO PLBS ORGANOGENESIS OF PHALAENOPSIS TO CARBOHYDRATE SOURCES UNDER LED LIGHTS ........... 190 T4-P31 INCIDENCE OF BEMISIA TABACI AND TRIALEURODES VAPORARIOURUM IN HYDROPONIC TOMATO CROP AS AFFECTED BY ROOTSTOCK........................................................................................................................................................................... 190 T4-P32 INDUCTOME: OPTIMIZING PLANT PERFORMANCE UNDER CONTROLLED STRESS CONDITIONS AIMING TOWARDS INCREASED ACCUMULATION OF SECONDARY METABOLITES IN TOMATO LEAVES.................................... 191 T4-P33 INFLUENCE OF DRIP IRRIGATION ON PEACH TREE GROWTH IN THE CHERNOZEM OF DOBROGEA, ROMANIA ...................................................................................................................................................................................................................... 191 T4-P34 INVITRO PROPAGATION OF `JUMBO´ BLACKBERRY CULTIVAR....................................................................................................... 192 T4-P35 IODINE BIOFORTIFICATION OF GREENHOUSE CUCUMBERS CULTIVATED IN A HYDROPONIC SYSTEM .................. 192 T4-P36 MODERN TRAINING SYSTEMS FOR SWEET CHERRIES -SLENDER SPINDLE OR HEDGEROW FOR PROTECTED GROWING?- .................................................................................................................................................................................................................. 193 T4-P37 MULCHING COMBINED WITH REGULATED DEFICIT IRRIGATION CAN BE SUCCESSFULLY APPLIED TO YOUNG PEAR TREES ................................................................................................................................................................................................ 193 T4-P38 NEW APPROACH OF ELECTROTHERAPY FOR GRAPEVINE VIRUS ELIMINATION .................................................................. 194 T4-P39 NON-INVASIVE DETECTION OF RUSSET ON POME FRUIT .................................................................................................................. 194 T4-P40 OPTIMIZATION OF LIGHT-EMITTING DIODES SPECTRUM FOR GREENHOUSE PLANT LIGHTING BASED ON RELATIVE QUANTUM EFFICIENCY .................................................................................................................................................................. 194 T4-P41 P)CLÉG': A 'RESEARC(, DEVELOPMENT AND STAKEHOLDERS' ALL-TOGETHER INITIATIVE FOR INTEGRATED PRODUCTION OF FIELD VEGETABLES IN FRANCE ................................................................................................... 195 T4-P42 POWER SUPPLY OF GREENHOUSES BY USING VOLATILE ELECTRICITY GRID WITH HIGH PROPORTION OF RENEWABLES ............................................................................................................................................................................................................. 195 T4-P43 PRODUCTIVITY OF BABY PLUM TOMATO CV SWEETELLE ................................................................................................................ 196 T4-P44 PROPAGATION OF SCABIOSA HYMETTIA (BOISS & SPRUNER) BY STEM CUTTINGS ............................................................ 196 T4-P45 PROTOCOL FOR RAPID IN VITRO SHOOT MULTIPLICATION OF ANCIENT APPLE CULTIVARS AND LANDRACES, GROWN IN CZECH REPUBLIC ................................................................................................................................................ 197 T4-P46 REDUCED ENERGY USAGE IN COMMERCIAL APPLE STORAGE WITH IMPROVED AIR CIRCULATION ......................... 197 236 III International Symposium on Horticulture in Europe – SHE 2016 T4-P47 RESEARCH ON FREEZING BEHAVIOR OF SOME CRANBERRY FRUIT GROWN IN ROMANIA ............................................. 198 T4-P48 SELECTIVE MECHANICAL THINNING TECHNOLOGY FOR HORTICULTURAL FRUIT CROPS ............................................. 198 T4-P49 STUDIES ON ROOTING PERFORMANCES OF CAROB (CERATONIA SILIQUA) CUTTINGS .................................................... 199 T4-P50 THE CONTROL OF EPICOMETIS HIRTA PODA IN APPLE ORCHARDS WITH AZADIRACHTIN ........................................... 199 T4-P51 THE EFFECT OF BIOSTIMULATORS TREATMENT UPON SEED BREEDING OF ASTER NOVI-BELGII............................. 200 T4-P52 THE EFFECT OF MODIFIED ATMOSPHERE PACKAGING ON POSTHARVEST PERFORMANCE OF TWO LOQUAT CULTIVARS ................................................................................................................................................................................................................... 201 T4-P53 UNRAVEL THE COMPLEXITY OF APPLE AROMA BY DYNAMIC VOCS FINGERPRINTING .................................................... 201 T4-P54 USE OF BENEFICIAL MICROORGANISMS TO IMPROVE ROOTING SUCCESS RATE IN CHAMAECYPARIS SP. CUTTINGS AND FUSARIUM RESILIENCE IN CYCLAMEN....................................................................................................................... 202 T4-P55 VALORIZATION OF DIGESTATE IN GREENHOUSE TOMATO CROPS............................................................................................... 202 T4-P56 YIELD AND QUALITY OF LETTUCE IN RESPONSE TO THE PLANT POSITION IN PHOTOVOLTAIC GREENHOUSE ............................................................................................................................................................................................................. 203 T4-P57 EFFECT OF INDOLEBUTYRIC ACID (IBA) AND CANE POSITON ON ROOTING OF ROOTSTOCK'S CUTTINGS ............ 203 Poster Topic – 5 Ornamentals, Landscape And Urban Environment .......................... 204 T5-P1 A STUDY ON CUT FLOWER POTENTIAL OF ANTALYA .......................................................................................................................... 205 T5-P2 ANTI-FEEDING ACTIVITY OF SELECTED BOTANICAL EXTRACTS AND THEIR COMBINATIONS ON PLUTELLA XYLOSTELLA LARVAE............................................................................................................................................................................................. 205 T5-P3 CHARACTERIZATION DESCRIPTORS FOR LITHUANIAN LOPHOSPERMUM CULTIVARS..................................................... 206 T5-P4 EFFECTS OF GIBBERELLIC ACID APPLICATIONS ON INDUCTION OF PARTHENOCARPY IN BLACK MYRTLE FRUITS ............................................................................................................................................................................................................................ 206 T5-P5 FLOWER COLOR AND FLAVONOID PIGMENTS COMPOSITION OF THE PETALS IN ORNAMENTAL PEACH CULTIVARS OBTAINED BY CROSSING WITH PRUNUS MIRA .............................................................................................................. 207 T5-P6 HOW CAN INNOVATION OF LOCALLY GROWN FRUIT AND VEGETABLES REDUCE FOOD WASTE IN THE PUBLIC PROCUREMENT PROCESS? ................................................................................................................................................................. 207 T5-P7 IDENTIFICATION OF SSR IN GARDENIA JASMINOIDES ELLIS DE NOVO TRANSCRIPTOMIC ANALYSIS ...................... 208 T5-P8 IMPORTANCE OF GEOGRAPHICAL INDICATIONS (GI) IN FRUIT PRODUCTION, CASE STUDY; ''FINIKE ORANGE'' ....................................................................................................................................................................................................................... 208 T5-P9 MORPHOLOGIC VARIABILITY IN PORTUGUESE MENTHA CERVINA ACCESSIONS ................................................................. 209 237 BOOK OF ABSTRACTS T5-P10 MULTI-SPECIES NEAR INFRARED CALIBRATION MODELS FOR PREDICTING NITROGEN STATUS OF ORNAMENTAL CUTTINGS ' STRATEGIES FOR SCOPE EXTENSION ................................................................................................. 209 T5-P11 NEW PEACH CULTIVARS BY NIKITA BOTANICAL GARDENS WITH HIGH RESISTANCE TO LEAF CURL (TAPHRINA DEFORMANS TUL.) ........................................................................................................................................................................ 210 T5-P12 OBSERVATIONS RELATED THE LONG-EARED OWL (ASIO OTUS) FEEDING IN TWO HORTICULTURAL ECOSYSTEMS ............................................................................................................................................................................................................... 210 T5-P13 ORNAMENTAL PEACH CULTIVARS TOLERANT TO POWDERY MILDEW AND LEAF CURL DISEASE ............................ 211 T5-P14 PLANT EXTRACT CONSTITUENTS FROM INSECTICIDAL EFFECTS OF CORIANDER, ORIENTAL VALERIAN, CINNAMON AND GALANGAL ON MAJOR PESTS ........................................................................................................................................ 211 T5-P15 RADIO FREQUENCY IDENTIFICATION SYSTEM PERFORMANCE IN ORNAMENTAL PLANT OPERATIONS ............... 212 T5-P16 RECENT DEVELOPMENTS IN THE APPLE WORLD MARKET: THE REGIONALIZING EFFECT OF THE PHYTOSANITARY BARRIERS? ............................................................................................................................................................................ 212 T5-P17 STERNBERGIA LUTEA DEVELOPMENT AT DIFFERENT NUTRIENT SOLUTION LEVELS ..................................................... 213 T5-P18 SYMPTOMS OF CU, ZN, MN AND B TOXICITY IN ORNAMENTAL VRIESEA CULTIVARS ........................................................ 213 T5-P19 THE BULBLET FORMATION OF GALANTHUS ELWESSI HOOK. IN DIFFERENT IBA AND NAA DOSES .......................... 214 T5-P20 THE EFFECTS OF FERTILIZATION ON THE GROWTH AND FLOWERING OF LILIUM REGALE SPECIES ...................... 214 T5-P21 THE HYBRIDIZATION OF WHITE FLESH NECTARINE WITH SOME SPECIES AFFECTING POMOLOGICAL PARAMETERS ............................................................................................................................................................................................................. 215 T5-P22 THE RESPONSE OF LEUCOJUM AESTIVUM L. TO DIFFERENT METHODS OF VEGETATIVE PROPAGATION ON BULBLET FORMATION AND GROWTH CHARACTERISTICS ................................................................................................................ 215 T5-P23 WATERCORE SEVERITY OF `FUJ)´ APPLES FROM DIFFERENT POSITIONS IN THE TREE CANOPY UNDER DIFFERENT CROP LOADS ..................................................................................................................................................................................... 215 T5-P24 WINTER-HARDY SWEET CHERRY CULTIVARS AND FORMS OF NIKITA BOTANICAL GARDEN ....................................... 216 238

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