Medicinal and Aromatic Plant Science and Biotechnology ©2007 Global Science Books
Clerodendrum and Heathcare: An Overview
Neeta Shrivastava* • Tejas Patel
B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, S. G. Highway, Thaltej, Ahmedabad - 380054, Gujarat, India
Corresponding author: * neetashrivastava_perd@yahoo.co.in
ABSTRACT
The genus Clerodendrum L. (Family: Lamiaceae) is very widely distributed in tropical and subtropical regions of the world. More than
five hundred species of the genus are identified till now, which includes small trees, shrubs and herbs. Ethno-medical importance of
various species of Clerodendrum genus has been reported in various indigenous systems of medicines and as folk medicines. The genus is
being used as medicines specifically in Indian, Chinese, Thai, Korean, Japanese systems of medicine for the treatment of various lifethreatening diseases such as syphilis, typhoid, cancer, jaundice and hypertension. Few species of the genus like Clerodendrum inerme, C.
thomosonae, C. indicum, and C. speciosum are ornamental and being cultivated for aesthetic purposes. The powder/paste form and the
various extracts of root, stem and leaves are reported to be used as medicine for the treatment of asthma, pyreticosis, cataract, malaria, and
diseases of blood, skin and lung. To prove these ethno-medical claims, some of these species are being extensively studied for their
biological activities using various animal models. Along with biological studies, isolation and identification studies of chemical constituents and its correlation with the biological activities of the genus has also been studied. The major chemical components reported from
the genus are phenolics, steroids, di- and triterpenes, flavonoids, volatile oils, etc. This review mainly covers the extent of work done on
biological activities of various Clerodendrum species such as C. trichotomum, C. bungei, C. chinense, C. colebrookianum, C. inerme, C.
phlomidis, C. petasites, C. grayi, C. indicum, C. serratum, C. campbellii, C. calamitosum and C. cyrtophyllum that can be used both in
conventional therapy or as replacement therapies for the treatment of various diseases.
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Keywords: ethnomedical, phytochemistry, anti-inflammatory, antimicrobial, antimalarial, antioxidant, antidiabetic, polyphyletic, paraphyletic
Abbreviations: AGC, apigenin-7-O-ȕ-D-glucoside; GSH, glutathione; MDA, malondialdehyde; PGE2, prostaglandin E2; XO, xanthine
oxidase
CONTENTS
INTRODUCTION...................................................................................................................................................................................... 142
ETHNOMEDICAL USES.......................................................................................................................................................................... 146
PHYTOCHEMISTRY................................................................................................................................................................................ 146
BIOLOGICAL ACTIVITIES ..................................................................................................................................................................... 147
Anti-inflammatory activities.................................................................................................................................................................. 147
Antimicrobial activites .......................................................................................................................................................................... 147
Antimalarial activities............................................................................................................................................................................ 148
Antioxidant activities............................................................................................................................................................................. 148
Other biological activities of Clerodendrum genus ............................................................................................................................... 148
SUMMARY ............................................................................................................................................................................................... 148
ACKNOWLEDGEMENT ......................................................................................................................................................................... 149
REFERENCES........................................................................................................................................................................................... 149
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INTRODUCTION
The genus Clerodendrum L. [Family Lamiaceae (Verbenaceae)] is very widely distributed in tropical and subtropical
regions of the world and is comprised of small trees, shrubs
and herbs. The first description of the genus was given by
Linnaeus in 1753, with identification of C. infortunatum.
After a decade later in 1763 Adanson changed the Latin
name "Clerodendrum" to its Greek form "Clerodendron";
in Greek Klero means chance and dendron means tree i.e.
chance tree which means the tree which does not bring
good luck like Clerodendron infortunatum or the tree which
brings good luck like C. fortunatum. Later on after a span
of about two centuries in 1942 Moldenke readopted the
Latinized name 'Clerodendrum', which is now commonly
used by taxonomists for the classification and description of
the genus and species (Moldenke 1985; Rueda 1993; Hsiao
and Lin 1995; Steane et al. 1999). Clerodendrum is a very
Received: 4 January, 2007. Accepted: 22 January, 2007.
large and diverse genus and till now five hundred and eighty
species of the genus have been identified and are widely
distributed in Asia, Australia, Africa and America (Table 1).
A high degree of morphological and cytological variation
(from 2n=24 to 2n=184) amongst the species, suggesting
the paraphyletic or polyphyletic origin of the genus. Molecular systematic studies based on cloroplast and nuclear
DNA also indicate polyphyletic origin of the genus (Steane
et al. 1999). Owing to morphological variations like length
of the corolla tube, size of leaves, and type of inflorescence
some authors have classified the genus into two major subgenera, Clerodendrum and Cyclonema (Steane et al. 1999)
while others have classified it into five subgenera and each
subgenus is again subdivided into many sections (Moldenke
1985). Similarly many species of the genus have been described by more than one author and hence are denoted in the
literature with the name of different authors e.g. C. floribundum Hort. and C. floribundum R.Br., C. foetidum Bunge
Invited Review
Medicinal and Aromatic Plant Science and Biotechnology 1(1), 142-150 ©2007 Global Science Books
Table 1 List of various species from the genus Clerodendrum. * Species described by more than one author.
C. cernuum Wall.ex Steud.
C. foetidum Bunge*
C. acerbiana Benth. & Hook.f.
C. chamaeriphes Wernham
C. formicarum Gurke
C. aculeatum (L.) Schlecht.*
C. citrinum Ridley
C. formosanum Maxim.
C. acuminatum Wall.
C. coccineum D.Dietr.*
C. fortunatum Buch.-Ham.ex Wall.*
C. adenocalyx Dop
C. cochinchinense Dop
C. fortunei Hemsl.
C. adenophysum H.Hallier
C. colebrookianum Walp.
C. fragrans Vent.*
C. affine Griff.
C. commersonii Spreng.
C. francavilleanum Buchinger ex
C. africanum Moldenke
C. condensatum Miq.
Berthold Thomas
C. aggregatum Gurke
C. confusum H.Hallier
C. friesii K.Schum.
C. alatum Gurke
C. congense Baker*
C. f.rutectorum S.Moore
C. albiflos H.J.Lam
C. congestum Guerke
C. fugitans Wernham
C. amicorum Seem.
C. conglobatum Baker
C. fuscum Gurke.
C. amplifolium S.Moore
C. consors S.Moore
C. galeatum Balf.f.
C. amplius Hance
C. corbisieri De Wild.
C. garrettianum Craib
C. anafense Britton & P.Wils.
C. gaudichaudii Dop
C. angolense Guerke
C. cordatum D.Don
C. geoffrayi Dop
C. angustifolium Salisb.*
C. cordifolium A.Rich.
C. giletii Wildem. & Th.Dur.
C. apayaoense Quisumb.
C. coriaceum Poir.*
C. glaberrimum Hayata
C. arenarium Baker
C. coromandelianum Spreng.
C. glabratum Guerke
C. arthur-gordoni Horne ex Baker
C. costaricense Standley
C. glabrum E.Mey.
C. assurgens K.Schum.
C. costatum R.Br.
C. glandulosum Colebr.ex Wall.
C. attenuatum De Wild.*
C. costulatum K.Schum.
C. glandulosum Lindl.
C. aucubifolium Hemsl.
C. cruentum Lindl.
C. glaucum Wall.ex Steud.
C. aurantiacum Baker
C. cubensis Schau.
C. globuliflorum Berthold Thomas
C. aurantium G.Don
C. culinare Sesse & Moc.
C. godefroyi Kuntze
C. × speciosum Teijsm. & Binn.
C. cumingianum Schau.
C. goossensi De Wild.
C. bakeri Gurke
C. cuneatum Guerke*
C. gordoni Baker
C. balfouri Hort.
C. cuneifolium Baker
C. gossweileri Exell
C. barbafelis H.Hallier
C. cunninghamii Benth.
C. grandicalyx E.A.Bruce
C. baronianum Oliver
C. curranii Elmer
C. grandiflorum Schau.
C. barteri Baker
C. curtisii N.E.Br.
C. grandifolium Gurke*
C. baumii Guerke
C. cuspidatum Turcz.
C. gratum Kurz*
C. bequaerti De Wild
C. cyrtophyllum Turcz
C. greyi Baker
C. bernieri Briq.
C. darrisii Leveille
C. griffithianum C.B.Clarke
C. bethuneanum Low
C. deflexum Wall.
C. guerkii Baker
C. bingaense S.Moore
C. dekindtii Guerke
C. haematocalyx Hance
C. bipindense Guerke
C. dembianense Chiov.
C. haematolasium H.Hallier
C. blancoanum Villar
C. densiflorum Griff.
C. hahnianum Dop
C. blancoi Naves ex Villar
C. dentatum Wall.
C. hainanense Hand.-Mazz.
C. blumeanum Schau.
C. depauperatum Wall.ex Steud.
C. harmandianum Dop
C. bodinierii Leveille
C. dependens Aug.DC.
C. harnierianum Schweinf.
C. bolivianum Rusby
C. dicolor Vatke
C. hastato-oblongum C.B.Clarke
C. botryoides Baker
C. diepenhorstii Miq.
C. hastatum Lindl.
C. botryoides K.Schum.
C. dinklagei Gurke
C. helianthemifolium Wall.ex Steud.
C. brachyanthum Schau.
C. discolor Becc.
C. hemiderma F.Muell.ex Benth.
C. brachypus Urb.
C. disparifolium Blume
C. henryi P'ei
C. lerodendrum bracteatum Wall.
C. divar. catum Jack*
C. herbaceum Wall.
C. bracteosum Kostel.
C. diversifolium Vahl
C. brassii Beer & H.J.Lam
C. dubium De Wild.
C. heterophyllum
C. brazzavillense A.Cheval.
C. duckei Moldenke
C. hettae H.Hallier
C. hexagonum De Wild
C. dumale Baker
C. breviflorum Ridl.
C. hexangulatum Berthold Thomas
C. dumale K.Schum.
C. brookeanum W.W.Smith
C. hildebrandtii Vatke
C. dusenii Guerke
C. brunfelsiiflorum H.Hallier
C. hircinum Schau.
C. eketense Wernham
C. brunsvigioides Baker
C. hirsutum G.Don*
C. ekmani Moldenke
C. buchananii Herb.Roxb.ex Wall.
C. hispidum M.R.Henderson
C. elberti H.Hallier
C. buchholzii Gurke
C. hockii De Wild.
C. elegans Manetti ex Lem.
C. buchneri Gurke
C. holstii Guerke ex Baker*
C. ellipticum Zipp.ex Span.
C. buettneri Gurke
C. holtzei F.Muell.
C. elliptifolium Merrill
C. bukobense Gurke
C. horsfieldii Miq.
C. elmeri Merrill
C. bungei Steud.
C. huegelii Hort.ex Regel
C. emarginatum Briq.
C. buruanum Miq.
C. humile Chiov.
C. emirnense Boj.ex Hook.
C. buxifolium Spreng.
C. hysteranthum Baker
C. epiphyticum Standley
C. cabrae De Wild.
C. illustre N.E.Br.
C. caeruleum N.E.Br.
C. erectum De Wild.
C. impensum Berthold Thomas
C. caesium Guerke
C. eriophyllum Gurke
C. imperialis Carr.
C. calamistratum Hort.Belg.ex Lem. C. eriosiphon Schau.
C. inaequipetiolatum Good
C. calamitosum Linn.
C. esquirolii Leveille*
C. incisum Klotzsch
C. calcicola Britton
C. eucalycinum Oliver
C. indeniense A.Cheval.
C. calycinum Turcz.
C. eupatorioides Baker
C. indicum Druce*
C. camagueyense Britton & P.Wils.
C. euryphyllum Mildbr.
C. inerme Gaertn.*
C. canescens Wall.
C. excavatum De Wild.
C. infortunatum Dennst.*
C. capense D.Don ex Steud.*
C. fallax Lindl.
C. ingratum K.Schum. & Lauterb.
C. capitatum Hook.*
C. fargesii Dode
C. intermedium Berthold Thomas*
C. capsulare Blanco
C. farinosum Wall.
C. involucratum Vatke
C. cardiophyllum F.Muell.
C. fasciculatum Berthold Thomas
C. ixoraeflorum Hazsk.
C. carnosulum Baker
C. fastigiatum H.J.Lam
C. jackianum Wall.
C. castaneaefolium Klotzsch
C. ferrugineum Turcz.
C. japonicum Mak.*
C. castaneifolium Hook. & Arn.
C. finetii Dop
C. javanicum Spreng.*
C. catalpifolium H.Hallier
C. fischeri Gurke ex Engl.
C. johnstoni Oliver
C. caulambum Exell
C. fistulosum Becc.*
C. kaempferi Fisch.ex Morr.
C. cauliflorum De Wild.*
C. flavum Merrill
C. kalaotoense H.J.Lam
C. cavaleriei Leveille
C. fleuryi A.Chevalier
C. kalbreyeri Baker
C. cephalanthum Oliver
C. floribundum Hort.*
143
C. kampotense Dop
C. kanichi Wildem.
C. katangensis Wildem.
C. kentrocaule Baker
C. kinabaluense Stapf
C. kirkii Baker
C. kissakense Guerke
C. klemmei Elmer
C. koshunense Hayata
C. kwangtungense Hand.-Mazz.
C. laciniatum Balf.f.
C. laevifolium Blume
C. lanceolatum F.Muell.
C. lanceolatum Gurke.
C. lanceoliferum S.Moore
C. lanessanii Dop
C. lankawiense King & Gamble
C. lanuginosum Blume
C. lasiocephalum C.B.Clarke
C. laxicymosum De Wild.
C. laxiflorum Baker
C. lecomtei Dop
C. lehuntei Horne ex Baker
C. lelyi Hutchinson
C. leucophloeum Balf.f.
C. leucosceptrum D.Don
C. leveillei Fedde ex Leveille
C. ligustrinum
C. lindawianum Lauterb.
C. lindemuthianum Vatke
C. lindenianum A.Eich.
C. lindleyi Decne.ex Planch.
C. linnaei F.Muell.*
C. lividum Lindl.
C. lloydianum Craib
C. lobbii C.B.Clarke
C. longicolle G.F.W.Mey.
C. longiflorum Decne.
C. longilimbum P'ei
C. longipetiolatum Gurke*
C. longisepalum Dop
C. longituba Valeton
C. longitubum Wildem. & Th.Dur.
C. luembense De Wild.
C. lujaei Wildem. & Th.Dur.
C. lupakense S.Moore
C. luzoniense Merrill
C. mabesae Merrill
C. macradenium Miq.
C. macrocalycinum Baker
C. macrocalyx De Wild.*
C. macrophyllum Blume*
C. macrosiphon Hook.f.*
C. macrostachyum Baker*
C. macrostegium Schau.
C. madaeera Voigt
C. magnificum Warb.
C. magnoliaefolium Baker
C. makanjanum H.Winkler
C. mandarinorum Diels
C. manetti Vis.
C. mannii Baker
C. margaritense Moldenke
C. matudae Standley
C. medium R.Br.
C. megasepalum Baker
C. melanocrater Gurke
C. membranifolium H.J.Lam
C. mexicanum T.S.Brandegee
C. meyeri-johannis Mildbraed
C. micans Gurke
C. microcalyx Ridley
C. microphyllum Berthold Thomas
C. mildbraedii Berthold Thomas
C. minahassae Teijsm. & Binn.
C. mindorense Merrill
C. minutiflorum Baker
C. mirabile Baker
C. mite Vatke
C. moldenkeanum Standley
C. molle H.B. & K.*
C. montanum Berthold Thomas
Clerodendron and healthcare. Shrivastava and Patel
Table 1 (cont.) * Species described by more than one author.
C. schultzei Mildbr.
C. morigono Chiov.
C. poggei Gurke
C. schweinfurthii Gurke
C. mossambicense Klotzsch
C. polyanthum Guerke
C. scopiferum Miq.
C. moupinense Franch.
C. polycephalum Baker
C. semiserratum Wall.
C. muenzneri Berthold Thomas
C. populneum Beer & H.J.Lam
C. multibracteatum Merrill
C. porphyrocalyx K.Schum. & Lauterb. C. sereti De Wild.
C. multiflorum G.Don
C. powellii Benth. & Hook.f.ex Drake C. sericeum Wall.
C. serotinum Carr.*
C. myrianthum Mildbr.
C. preslii Elmer
C. serratum Moon*
C. myricoides Gurke*
C. preussii Gurke.
C. sieboldii Kuntze
C. myrmecophila Ridl.
C. prittwitzii Berthold Thomas
C. silvaeanum Henriques
C. natalense Gurke
C. puberulum Merrill
C. silvestre Berthold Thomas
C. navesianum Vidal
C. pubescens Lindl.
C. silvicola Guerke.
C. nereifolium Wall.
C. pubescens Walp.
C. simile H.H.W.Pearson*
C. neumayeri Vatke
C. pulchrum Fawc.
C. simplex G.Don
C. nhatrangense Dop
C. pulverulentum Engl.
C. singalense Miq.
C. nipense Urb.
C. pumilum Ridley
C. singwanum Berthold Thomas
C. noiroti A.Chevalier
C. pumilum Spreng.
C. sinuatum Hook.
C. nutans Jack*
C. pusillum Guerke
C. siphonanthus
C. nyctaginifolium Good
C. putre Schau.
C. somalense Chiov.
C. obanense Wernham
C. pygmaeum Merrill
C. speciosissimum Hort.Angl.ex
C. obovatum Walp.
C. pynaertii De Wild.
Schau.
C. obtusidens Miq.
C. pyramidale Andr.
C. odoratum D.Don
C. quadrangulatum Berthold Thomas C. speciosum Guerke*
C. spicatum Thunb.
C. ohwii Kanehira & Hatusima
C. quadriloculare Merrill
C. spinescens Gurke
C. orbiculare Baker
C. ramosissimum Baker
C. spinosum Spreng.
C. oreadum S.Moore
C. reflexum H.H.W.Pearson
C. splendens A.Cheval.*
C. ornatum Wall.
C. rehmannii Guerke
C. splendidum Wall.
C. ovale Klotzsch
C. rhytidophyllum K.Schum.
C. squamatum Vahl
C. ovalifolium A.Gray*
C. ridleyi King & Gamble
C. squiresii Merrill
C. ovatum Poir.*
C. riedelii Oliver
C. stenanthum Klotzsch
C. oxysepalum Miq.
C. ringoeti De Wild.
C. palmatolobatum Dop
C. robecchii Chiov.
C. streptocaulon Hutchinson &
C. paniculatum Linn.
C. robinsonii Dop
Dalziel
C. papuanum Scheff.
C. robustum Klotzsch
C. strictum Baker
C. parvitubulatum Berthold Thomas C. roseum Poit.
C. stuhlmanni Gurke
C. pearsoni Moldenke
C. rotundifolium Oliver
C. subpandurifolium Kuntze
C. peekelii Markgraf
C. rubellum Baker
C. subpeltatum Wernham
C. penduliflorum Wall.
C. rumphianum Bull
C. subreniforme Guerke
C. pentagonum Hance
C. rumphianum De Vriese
C. subscaposum Hemsl.
C. petasites S.Moore
C. rusbyi Moldenke
C. suffruticosum Guerke
C. petunioides Baker
C. sagittatum Wall.
C. swynnertonii S.Moore
C. philippinense Elmer
C. sagraei Schau.
C. sylvaticum Briq.
C. philippinum Schau.
C. syringaefolium Baker
C. sahelangii Koord.ex Bakh.
C. phlebodes C.H.Wright
C. talbotii Wernham
C. sanguineum K.Schum.
C. phlomoides Hort.Ital.ex DC.*
C. tanganyikense Baker
C. sansibarense Gurke
C. phyllomega Steud.
C. tatomense Dop
C. sarawakanum H.J.Lam
C. picardae Urb.
C. teaguei Hutchinson
C. savanorum De Wild.
C. pierreanum Dop
C. ternatum Schinz
C. scandens Beauv.*
C. pilosum H.H.W.Pearson
C. ternifolium Baker*
C. scheffleri Guerke*
C. pithecobium Standley & Steyerm. C. schlechteri Guerke
C. tessmanni Moldenke
C. pittieri Moldenke ex Standley
C. thomasii Moldenke
C. schliebenii Mildbr.
C. thonneri Guerke
C. schmidtii C.B.Clarke
C. pleiosciadium Gurke
C. thyrsoideum Baker*
C. tomentellum Hutchinson & Dalziel
C. tomentosum R.Br.
C. tonkinense Dop
C. toxicarium Baker*
C. tracyanum F.Muell.ex Benth
C. transvaalense Berthold Thomas
C. tricholobum Guerke
C. trichotomum Thunb.*
C. triflorum Vis.
C. trifoliatum Steud.
C. triphyllum H.H.W.Pearson
C. triplinerve Rolfe
C. tuberculatum A.Rich.
C. ubanghense A.Chevalier
C. ugandense Prain
C. ulei Hayek
C. ulugurense Guerke
C. umbellatum Poir.
C. umbratile King & Gamble
C. uncinatum Schinz
C. urticifolium Wall.
C. utakwense Wernham
C. validipes S.Moore
C. vanoverberghii Merril
C. vanprukii Craib
C. var. ifolium De Wild.
C. var. um Berthold Thomas
C. velutinum A.Chevalier
C. velutinum Berthold Thomas*
C. venosum Wall.
C. verrucosum Splitg.ex De Vriese
C. versteegi Pulle
C. verticillatum D.Don
C. vestitum Wall.ex Steud.
C. villosum Blume
C. violaceum Guerke*
C. viscosum Vent.
C. volubile Beauv.
C. weinlandii K.Schum.ex H.J.Lam
C. welwitschii Gurke
C. wenzelii Merrill
C. whitfieldii Seem.*
C. wildemanianum Exell
C. williamsii Elmer
C. wilmsii Guerke
C. yakusimense Nakai
C. yatschuense H.Winkler
C. yaundense Guerke
C. yunnanense Hu
C. zambesiacum Baker
Table 2 A few species of the Clerodendrum genus described by many authors.
C. floribundum Hort.
C. infortunatum Dennst.
C. aculeatum (L.) Schlecht.
C. floribundum R.Br.
C. infortunatum Gaertn.
C. aculeatum Griseb.
C. foetidum Bunge
C. infortunatum Linn.
C. angustifolium Salisb.
C. foetidum D.Don
C. intermedium Berthold Thomas
C. angustifolium Spreng.
C. foetidum Hort.Par.ex Planch.
C. intermedium Cham.
C. attenuatum De Wild.
C. fortunatum Buch.-Ham.ex Wall.
C. japonicum Mak.
C. attenuatum R.Br.
C. fortunatum Linn.
C. japonicum Sweet
C. capense D.Don ex Steud.
C. javanicum Spreng.
C. capense Eckl. & Zeyh.ex Schau. C. fragrans Vent.
C. fragrans Willd.
C. javanicum Walp.
C. capitatum Hook.
C. glandulosum Colebr.ex Wall.
C. linnaei F.Muell.
C. capitatum Schum & Thou.
C. glandulosum Lindl.
C. linnaei Thw.
C. cauliflorum De Wild.
C. grandiflorum Schau.
C. cauliflorum Vatke
C. macrocalyx De Wild.
C. grandifolium Gurke
C. coccineum D.Dietr.
C. macrocalyx H.J.Lam
C. grandifolium Salisb.
C. coccineum H.J.Lam
C. macrophyllum Blume
C. gratum Kurz
C. congense Baker
C. macrophyllum Sims
C. gratum Wall.
C. congense Engl.
C. molle H.B. & K.
C. hirsutum G.Don
C. coriaceum Poir.
C. molle Jack
C. hirsutum H.H.W.Pearson
C. coriaceum R.Br.
C. myricoides Gurke
C. holstii Guerke ex Baker
C. divar. catum Jack
C. myricoides R.Br. & Vatke
C. divar. catum Sieb. & Zucc.
C. nutans Jack
C. holstii Gurke.
C. fistulosum Becc.
C. nutans Wall.
C. indicum Druce
C. fistulosum Bower
C. ovalifolium A.Gray
C. indicum Kuntze
C. ovalifolium Bakh.
C. ovalifolium Engl.
C. ovatum Poir.
C. ovatum R.Br
C. scandens Beauv.
C. scandens Druce
C. scandens Linn.ex Jackson
C. scheffleri Guerke
C. schifferi A.Cheval.
C. serratum Moon
C. serratum Spreng.
C. simile H.H.W.Pearson
C. simile Merrill
C. ternifolium D.Don
C. ternifolium H.B. & K.
C. thyrsoideum Baker
C. thyrsoideum Guerke
C. toxicarium Baker
C. toxicarium Baker ex Gurke
C. velutinum A.Chevalier
C. velutinum Berthold Thomas
C. velutinum Wall.
and C. foetidum D. Don, C. lanceolatum F. Muell. and C.
lanceolatum Gurke, etc.; some more examples are cited in
Table 2 (Rueda 1993; Hsiao and Lin 1995; Steane et al.
1999). Conclusive remarks on the origin and classification
of the genus are still lacking and a thorough revision of the
classification of this genus supported by molecular systematics has been suggested by some researchers (Steane et al.
1999, 2004).
144
Medicinal and Aromatic Plant Science and Biotechnology 1(1), 142-150 ©2007 Global Science Books
OH
HO
HO
OH
OH
O
O
O
O
H
OCH2CH3
H3CO
O
OH
HO
OH
5-O-ethylclerodendricin
O
O
HO
Hispudilin
HO
OH
Iridiod diglucoside
HOH2CH2C
CH2CH2OH
O
Bungein
H
CN
HO
OH
COOH
HO
O
Colebrin
OH
OH
O
O
1(R) Lucumin
O
H
OH
HO
O
OH
O
HO
O
O
O
O
O
OH
OH
HO
COOH
Clerodermic acid
O
H3 C
OCH3
Jionoside D
HO
HO
O
OH
CN
OH
OH
HO
O
OH
O
OH
2(R) Prunasin
HO
OH
OH
O
O
Uncinatone
OH
RO
O
Apigenin
O
OCH3
OH
R = Gluc. acid (6-OMe)
O
Acacetin-7-O-methylglucuronate
Clerosterol
HOOC
O
OR'''
RO
COOH
R'O
CH3O
CH3O
OR''
Neolignan I
Neolignan III
R R', R" R''' = -CH2-
R R' = CH3, R" R'" = -CH2-
HO
Serratagenic acid
Neolignan II
R R' = -CH2-, R" R'" = CH3
O
HO
O
O
OH
O
O
OH
HO
H3C
OH
O
HO
OH
HOOC
Verbacoside
OH
HO
HO
HO
OH
O
O
OH
HO
OH
O
Scutellarin
Fig. 1 Some of the major chemical constituents of Clerodendrum genus.
145
Clerodendron and healthcare. Shrivastava and Patel
calamitosum was used as a medicine for the treatment of
kidney, gall and bladder stones. This plant is also reported to
have diuretic and antibacterial properties (Cheng et al.
2001). In the Chinese system of medicine C. bungei is used
for the treatment of headaches, dizziness, furuncles and hysteroptosis (Zhou et al. 1982; Yang et al. 2002). In India,
fruits of C. petasites are used to produce sterility, while in
China the plant is used as medicine for malaria (Hazekamp
et al. 2001; Panthong et al. 2003). Leaves of C. buchholzii
are reported in African pharmacopeia for treatment of furunculosis, echymosis and gastritis (Nyegue et al. 2005). Other
then their therapeutic use, some of the species of the genus
such as C. inerme, C. thomosonae, C. indicum and C. speciosum are also cultivated and used as ornamental plants.
The genus is taxonomically characterized by its entire
or toothed, oppositely arranged leaves, terete stems,
terminally or axillary cymose inflorescence, hypogynous
bisexual flowers, persistent calyx, cylindrical corolla tube
with spreading 5-lobed at the top, exerted stamens, short bifided stigma, imperfectly 4-celled ovary, exalbumenous
seeds and endocarp separating into 4 stony pyrenes (Kirtikar and Basu 1991; Hsiao et al. 1995; Steane et al. 1999).
Resembling its taxonomic diversity, the genus exhibits a
wide spectrum of folk and indigenous medicinal uses. Research is advancing towards scientific validation of classical therapeutic claims of the genus. In the present review
we have focused on the medicinal and health care aspects
of the genus. We have also included the work done on the
phytochemical constituent responsible or believed to be responsible for the therapeutic properties of various species
belong to the genus (Fig. 1).
PHYTOCHEMISTRY
ETHNOMEDICAL USES
A number of species from this genus were documented to
be used as folk medicine by various tribes in Asian and
African continents (Table 3). Many species of the genus
have also been documented in traditional systems of medicine practiced in countries like India, China, Korea, Thailand and Japan.
Roots and leaf extracts of C. indicum, C. phlomidis, C.
serratum, C. trichotomum, C. chinense and C. petasites
have been used for the treatment of rheumatism, asthma
and other inflammatory diseases (Anonymous 1992; Hazekamp et al. 2001; Kang et al. 2003; Panthong et al. 2003;
Choi et al. 2004; Sungwook et al. 2004; Kanchanapoom et
al. 2005). Plant species such as C. indicum and C. inerme
were used to treat coughs, serofulous infection, buboes
problem, venereal infections, skin diseases and as a vermifuge, febrifuge and also to treat Beriberi disease (Anonymous 1992; Rehman et al. 1997; Kanchanapoom et al.
2001). It was also reported that tribals use C. inerme as an
antidote of poisoning from fish, crabs and toads (Rehman et
al. 1997; Kanchanapoom et al. 2001; Pandey et al. 2003). C.
phlomidis, C. colebrookianum, C. calamitosum and C. trichotomum have been reported to have antidiabetic, antihypertensive and sedative properties (Singh et al. 1980;
Chaturvedi et al. 1984; Khan et al. 1996; Cheng et al. 2001;
Kang et al. 2003; Chae et al. 2004; Choi et al. 2004). C.
cyrtophyllum and C. chinense were used for the treatment
of fever, jaundice, typhoid and syphilis (Cheng et al. 2001;
Kanchanapoom et al. 2005). Roots, leaves and fresh juice
of leaves of C. infortunatum were used in eliminating ascarids and tumors, and also as a laxative (Anonymous 1992).
C. phlomidis has been used as an astringent and also in the
treatment of gonorrhea (Rani et al. 1999; Murugesan et al.
2001). The roots of C. serratum have been claimed to be
used in dyspepsia, seeds in dropsy and leaves as a febrifuge
and in cephalagia and ophthalmia (Anonymous 1992). C.
As mentioned earlier the genus Clerodendrum is reported in
various indigenous systems of medicine throughout the
world for the treatment of various diseases. Efforts have
been made by various researchers to isolate and identify
biologically active principle and other major chemical
constituents from various species of the genus. Research
reports on the genus denote that the major class of chemical
constituents present in the Clerodendrum genus are steroids
such as ȕ-sitosterol, Ȗ-sitosterol octacosanol, clerosterol,
bungein A, acteoside, betulinic acid, clerosterol 3-O-E-Dglucopyranoside, colebrin A-E, campesterol, 4Į-methylsterol, cholesta-5-22-25-trien-3-ȕ-ol, 24-ȕ-cholesta-5-22-25trine, cholestanol, 24-methyl-22-dihydrocholestanol, 24-ȕ22-25-bis-dehydrocholesterol,
24-Į-methyl-22-dehydrocholesterol, 24-ȕ-methyl-22-dehydrocholesterol, 24-ethyl22-dehydrocholesterol, 24-ethylcholesterol, 22-dehydroclerosterol, 24-methyllathosterol, 24-ȕ-ethyl-25-dehydrolathosterol, (24S)-ethylcholesta-5-22-25-triene-3ȕ-ol have
been isolated from various Clerodendron species such as C.
inerme, C. phlomidis, C. infortunatum, C. paniculatum, C.
cyrtophyllum, C. fragrans, C. splendens, C. campbellii and
C. splendens (Bolger et al. 1970; Abdul-Alim 1971; Joshi et
al. 1979; Sinha et al. 1980; Singh and Singhi 1981; Sinha et
al. 1982; Hsu et al. 1983; Singh and Prakash 1983; Singh
and Singhi 1983; Pinto and Nes 1985; Rempler and Hunkler
1986; Akihisa et al. 1989; Att-Ur-Rehman et al. 1997; Goswami et al. 1996; Yang et al. 2000; Kanchanapoom et al.
2001; Yang et al. 2002; Gao et al. 2003a, 2003b; Pandey et
al. 2003; Kanchanapoom et al. 2005; Lee et al. 2006).
Another class of constituents are terpenes which include: monoterpenes, diterpenes, triterpenes, iridoids and
sesquiterpenes. Terpenes such as D-amyrin, ȕ-amyrin, caryoptin, 3-epicaryoptin, 16-hydroxy epicaryoptin, clerodendrin A, B and C, clerodin, clerodermic acid, cleroinermin,
friedelin, gramisterol, iridoids (inerminoside A, B, C and D,
melittaside, monomelittoside, sammangaoside, ugandoside,
8-O-acetylmioporoside), obtusifoliol, oleanolic acid, royleanone, dehydroroyleanone, sesquiterpene (sammangaoside A,
Table 3 A few species of Clerodendrum genus and their distribution in the world.
Scientific Name
Synonym
Distribution
India, Sri Lanka, South East Asian countries, Australia, Pacific Islands
C. inerme Gaertn.
India
C. multiforum Burm. f.
C. phlomidis Linn. f.
India
C. serratum Spreng.
The Philippines
C. infortunatum Linn.
India
C. indicum (Linn) Kuntze
C. siphonanthus R. Br.
China
C. commersonii Spreng.
Southern Africa
C. glabrum E. Mey.
Southern Africa
C. triphyllum R. Br.
China, Korea, Japan
C. trichotomum
China
C. bungei Stued.
Indonesia, Taiwan
C. calamitosum L.
Taiwan
C. cyrtophyllum Turez.
Tropical regions of Asia
C. fragrans (Vent.) Willd.
C. chinense (Osb.) Mabberley
India, South Asian countries
C. colebrookianum
South Africa
C. myricoides
India, Malaysia, Sri Lanka, Vietnam, Southern China
C. petasites S. Moore
Queensland, Australia
C. philippinum Schauer
Southern Africa
C. heterophyllum R. Br. & Thb.
146
Medicinal and Aromatic Plant Science and Biotechnology 1(1), 142-150 ©2007 Global Science Books
B) clerodendrin A, uncinatone, Mi saponins-A, friedelanone, lupeol, betulinic acid, royleanone and dehydroroyleanone, and betulin have till now been isolated from various Clerodendron species such as C. inerme, C. phlomidis,
C. paniculatum, C. colebrookianum, C. wildii, C. uncinatum, C. mandarinorum, C. thomsonae, C. fragrans, C.
ugandense, C. chinense (Joshi et al. 1979; Sharma and
Singh 1979; Singh et al. 1981; Sinha et al. 1981; Seth et al.
1982; Singh and Prakash 1983; Achari et al. 1990; Raha et
al. 1991; Achari et al. 1992; Rao et al. 1993; Calis et al.
1994; El-Shamy et al. 1996; Kawai et al. 1998; Hazekamp
2001; Kanchanapoom et al. 2001; Yang et al. 2002; Kumari
et al. 2003; Chae et al. 2004; Dorsaz et al. 2004; Nishida et
al. 2004; Min et al. 2005).
Flavonoids are another class of compounds which are
mainly present in Clerodendron speices and they are also
responsible for few biological activities. The major flavonoids present are cynaroside, 5-hydroxy-4’-7-dimethoxy
methyl flavone, kaempferol, salvigenin, 4-methyl scutellarein, 5,7,4 O-trihydroxyflavone, apigenin, luteolin, acacetin-7-O-glucuronide, hispidulin, 2’-4-4’trihydroxy-6’methyl
chalcone, 7-hydroxy flavone, luteolin, naringin-4’-O-Į-glucopyranoside, pectolinarigenin, cirsimaritin, cirsimaritin-4’glucoside, quercetin-3-methyl ether which were isolated
from C. inerme, C. phlomidis, C. petasites, C. trichotomum,
C. mandarinorum, and C. infortunatum (Vendatham et al.
1977; Seth et al. 1982; Raha et al. 1989; Achari et al. 1990;
Raha et al. 1991; Roy and Pandey 1994, 1995; Roy et al.
1995 ; El-Shamy et al. 1996; Anam 1997, 1999).
There are also other chemical constituents present
which include volatile constituents such as 5-O-ethylcleroindicin D, linalool, benzyl acetate and benzyl benzoate,
which have been isolated from C. canescens, C. cyrtophyllum, C. inerme and C. philippinum (Yang et al. 2002; Nyegue et al. 2004; Wong and Tan 2005).
Other chemical constituent includes cyanogenic glycosides such as lucumin and prunasin which were isolated
from C. grayi (Miller et al. 2006). Phenolic compounds like
ȕ-benzyl alcohol, ȕ-benzyl alcohol-D-glucoside, neolignan,
darendoside-B, phenyl propanoids like (isovarbascoside,
verbascoside, leucosceptoside), vanillic acid, anisic acid,
para-hydroxy benzoic acid, gallic acid have been reported
in C. inerme, C. bungei and C. dauricum (Liu and Fu 1980;
Gabriele and Rimpler 1981; Zhou et al. 1982; Gabriele et al.
1983; Sakurai and Kato 1983; Calis et al. 1994); D-mannitol from C. serratum (Garg and Verma 2006). Carbohydrates like glucose, fructose, sucrose are been reported in C.
mandarinorum and C. inerme. Other constituents such as
ribosome-inactivating protein, salidroside, jinoside-D, acetoside have been isolated from C. inerme (Olivieri et al.
1996), while trichotomoside, cytotoxic pheophorbides and
cleromyrin-I have been isolated from C. trichotomum, C.
calamitosum and C. cyrtophyllum (Bashwira et al. 1989;
Cheng et al. 2001; Chae et al. 2006).
BIOLOGICAL ACTIVITIES
The genus Clerodendrum contains many plant species that
are being used in various health care systems for the treatment of various disorders including life-threatening diseases. To validate traditional claims associated with the genus
many studies are being carried out using various animal
models and in vitro assays. These studies showed that the
different species of the genus possess potent anti-inflammatory, antidiabetic, antimalarial, antiviral, antihypertensive, hypolipidemic and antioxidant activities and have potential to be developed as potent remedial agents from natural resources. Some major activities are described below.
Anti-inflammatory activities
Inflammation is a very complex pathophysiological process
involving a variety of biomoleucles responsible for causing
it such as leucocytes, macrophages, mast cells, platelets and
lymphocytes by releasing eicosanoids and nitric oxide. Pro147
inflammatory cytokines such as TNF- and IL-1 are also
responsible for various inflammatory conditions. Many species of the genus Clerodendrum showed potent anti-inflammatory activity. C. phlomidis was reported for significantly
decreasing paw oedemas induced by carrageenan in rats at a
dose of 1g/kg (Surendrakumar 1988). Similarly C. petasites
was reported to show moderate anti-inflammatory activity
in the acute phase of inflammation in rats. The ED50 values
of the experiment were reported to be 2.34 mg/ear and
420.41 mg/kg in rats (Panthong et al. 2003), it has been suggested by the authors that the anti-inflammatory activity of
the plant extract could be due to the inhibition of prostaglandin synthesis by the extract.
The anti-inflammatory activity of C. trichotomum leaves were checked in rat, mice and Raw 264.7 macrophage
cells using experimental models with 1 mg/kg solution of
30% and 60% methanolic extracts of leaves. Experimental
results concluded that inhibition by methanolic extract was
comparable to that of the positive control in an acute inflammation model, while in the chronic model the extract
showed 10% higher activity than the positive control. It also
suppressed the levels of prostaglandin E2 (PGE2) in RAW
264.7 macrophage cells (Choi et al. 2004). A phenyl propanoid glycoside 'acetoside' isolated from C. trichotomum also
showed anti-inflammatory activity by inhibiting the release
of histamine, arachidonic acid and prostaglandin E2 in RBL
2H3 cells. The mechanism identified for the inhibition of
histamine release was related to calcium concentration (Lee
et al. 2006).
Xanthine oxidase (XO) is the enzyme responsible for
the formation of uric acid from the purines hypoxanthine
and xanthine, and is responsible for the medical condition,
gout. Gout is caused by the deposition of uric acid in the
joints leading to painful inflammation. Purified hydroalcoholic extracts of leaves and branches of C. floribundum
showed 84% inhibition of XO activity (Sweeney et al.
2001). Results of the experiment indicate the potential of
the plant species to be developed as a remedy for XO-induced diseases.
Flavonoid glycosides of C. inerme showed modulation
in calcium transport in isolated inflamed rat liver and thereby showed reduction in inflammation. The results obtained
in the experiment were comparable with indomethacine
used as a positive control (Somasundram and Sadique 1986).
The alcoholic extract of roots of C. serratum showed a significant anti-inflammatory activity in carrageenan and also
in the cotton pellet model in experimental mice, rats and
rabbits (Narayanan et al. 1999).
Antimicrobial activites
Antiinfective compounds from natural resources are of great
interest as the existing drugs are getting less effective due to
increased tolerance of microorganisms. A number of species
from the genus Clerodendrum were documented in ancient
texts for their antimicrobial action. To validate these claims,
research work was carried out with various Gram positive
and Gram negative bacterial strains and also with fungal and
viral pathogens. Dried, aerial parts of C. inerme showed potent antiviral activity against Hepatitis B virus with an ED50
value of 16 μg/ml (Mehdi et al. 1997). Essential oil obtained from leaves of the plant showed antifungal activity against variety of fungal species such as Alternaria species,
Aspergillus species, Cladosporium herbarum, Cunnimghamella echinulata, Helminthosporium saccharii, Microsporum gypseum, Mucor mucedo, Penicillium digitatum, Rhizopus nigricans, Trichophyton rubrum and Trichothecium roseum (Sharma and Singh 1979). Alcoholic extracts of leaves
and flowers of C. inerme also exhibited antibacterial activity
against Escherichia coli and Staphylococcus aureus (George
and Pandalai 1949). Pectolinarigenin and chalcone glucoside isolated from leaf of C. phlomidis showed antifungal
activity (Roy et al. 1995).
Two phenyl propanoid glycosides (acteoside and acteoside isomer) isolated from C. trichotomum showed potent
Clerodendron and healthcare. Shrivastava and Patel
inhibition of HIV-1 integrase with IC50 values of 7.8 ± 3.6
and 13.7 ± 6.0 μM (Kim et al. 2001). A new hydroquinone
diterpenoid was isolated from C. uncinatum and was
strongly fungi toxic to the spores of Cladosporium cucumerinum (Dorsaz et al. 2004). Hexane extracts of C. colebrookianum at concentrations of 1000 and 2000 ppm
showed strong antibacterial activities against various Gram
positive and Gram negative pathogens such as S. aureus,
Staphylococcus haemolyticus, E. coli, Pseudomonas aeruginosa (Misra et al. 1995).
Two flavonoids from roots of C. infortunatum, cabruvin
and quercetin, showed strong antifungal activity. The former showed activity against Alternaria carthami and Helminthosporin oryzae, the latter against Alternaria alternate
and Fusarium lini at concentrations of 200, 500 and 1000
mg/ml (Roy et al. 1996). Mi-saponin-A, a triterpenoid saponin isolated from the roots of C. wildii, showed potent
antifungal activity against Cladosporium cucumerinum (Toyoto et al. 1990).
ing reflux oesophagitis and gastritis and may therefore be a
promising drug for their treatment (Min et al. 2005). In present lifestyles where stress has taken an unwanted important
position leading to excess production of free radicals these
natural remedies will prove a support to our biological system to balance metabolism.
Other biological activities of Clerodendrum genus
Antimalarial activities
In various ancient literatures related to healthcare Clerodendrum have been reported for its antimalarial activities because of the presence of a bitter principle. Studies with different parasites support these ancient claims. The alcoholic
extract of C. phlomidis showed antimalarial activity against
Plasmodium falciparum with an IC50 value of 48 μg/ml
(Simonsen et al. 2001). Another Indian species, C. inerme
also inhibited the growth of larvae of Ades aegypti, Culex
quinquefasciatus and Culex pipiens at 80 and 100 ppm
concentration of petroleum ether and ether extracts (Gayar
and Shazll 1968; Kalyanasundaram and Das 1985). C. myricoides a species from Southern Africa was also tested positive for its antimalarial activity against both sensitive and
resistant strains of P. falciparum with IC50< 30 μg/ml (Muregi et al. 2004), it also showed 31.7% suppression in parasitaemia against cloroquine tolerant strain of Plasmodium
berghei NK65 (Muregi et al. 2007). These plants may be
useful as a source for novel anti-plasmodial drugs/compounds from natural origin.
Antioxidant activities
Antioxidant compounds are responsible for scavenging free
radicals, which are produced during normal metabolism or
during adverse conditions that can be harmful to biological
systems and leading to death of an organism. Species like C.
inerme have been used as antioxidant drugs in various indigenous systems of medicines (Masuda et al. 1999). Organic and aqueous extracts of C. colebrookianum showed significant inhibition of lipid peroxidation in vitro and in vivo
induced by FeSO4-ascorbate in rats. Aqueous extracts
showed strongest inhibitory activity over organic extracts.
This lends scientific support to the therapeutic use of the
plant leaves claimed in tribal medicine (Rajlakshmi et al.
2003). Isoacteoside, trichotomoside and jionoside D, three
compounds isolated from C. trichotomum, when tested
showed significant scavenging activity of intracellular reactive oxygen species produced by hydrogen peroxide suggesting their antioxidant properties (Chae et al. 2004, 2005,
2006). Apigenin-7-O-ȕ-D-glucuronopyranoside (AGC), isolated from C. trichotomum leaves decreased the volume
of gastric juice and increased the gastric pH in a dose-dependent manner, decreasing the number of gastric lesions.
A malondialdehyde (MDA) level, which is the end product
of lipid peroxidation, was also decreased by AGC (i.d. 3
mg/kg), which increased significantly after the induction of
reflux oesophagitis. The MDA levels did not decrease when
either apigenin or omeprazole were used as a control suggesting that AGC has an antioxidative mechanism to reduce
gastric lesions. Apigenin glucuronopyranoside also decreased mucosal glutathione (GSH) levels significantly suggesting that AGC possesses free radical scavenging activity.
So it can be concluded that AGC is more potent in inhibit148
Other major biological activites reported for this genus are
antihypertensive, antitumor, antidiabetic, antihyperlipidemic,
larvicidal, antidiarrhoel activities. Organic extracts of C.
inerme showed strong uterine stimulant activity when tested
in female rats and rabbits (Sharaf et al. 1969), and also
showed strong antihemolytic activity in human adults at
0.02-2.0 mg/ml, with inhibition of phospholipase at 0.05-1.5
mg/ml (Somasundaram and Sidique 1986). The methanolic
extract of C. multiflorum leaves showed antidiarrhoeal activity against castor oil-induced diarrhoea, PGE2-induced
enteropooling and caused reduction in gastrointestinal
motility in rats (Rani et al. 1999), while leaf juice at 0.1%
showed anthelmentic activity against Ascaris lumbricoides,
Phreitima posthuma and Taenia solium (Garg and Sidique
1992). Two compounds, isoacteoside and jionoside D isolated from C. trichotomum also reduced the levels of apoptotic cells induced by the action of hydrogen peroxide (Chae
et al. 2004, 2005). C. bungei showed antitumor activity in
hepatic cells of mice at a dose of 100 g/kg (Shi et al. 1993).
CNS-related activities were also observed in C. phlomidis
showing tranquillizing, CNS depressant, muscle relaxant
and psychopharmacological effects in experimental mice
and rats (Murugesan et al. 2001). C. mandarinorum root extracts showed strong binding with opiate, adenosine-1, -2adrenergic, 5HT-1, 5HT-2, dopamine-2, histamine-1, GABA
(A), and GABA (B) receptors. Isolated compounds of these
plants showed weak binding with these recepters suggesting
its synergestic effect (Zhu et al. 1996). C. inerme extracts
showed hypotensive effects in dogs at 50 mg/kg (Bhakuni et
al. 1969).
A decoction of the entire C. phlomidis plant has been reported to have antidiabetic activity. A dose of 1 g/kg showed
antidiabetic effects in epinephrine and alloxan induced
hyperglycemia in rats and it also showed antihyperglycemic
activity in human adults at a dose of 15-30 g/day (Chaturvedi et al. 1984). Organic and crude extracts of C. colebrookianum significantly lowered the serum lipid profile in
rats suggesting that it has cardioprotective potential (Devi
and Sharma 2004). The methanolic extract of C. phlomidis
and leaf extracts of C. inerme showed antispasmodic activity in mouse (200 mg/kg; Murugesan et al. 2001) and guinea
pigs (2 mg/ml; Cox et al. 1989). Ethanolic extract (2.25-9.0
mg/ml) of C. petasites evaluated for spasmolytic activity in
guinea-pigs showed spasmolysis on tracheal smooth muscles; it also relaxed the smooth muscle which was contracted by exposure to histamine. The activity of smooth muscle
relaxation was attributed to hispidulin (flavonoid) with an
EC50 (3.0 ± 0.8 * 10-5 M) suggesting hispidulin has anti-inflammatory activity (Hazekamp 2001). Dichloromethane
leaf extracts of C. myricoides indicated antimutagenic properties against Salmonella typhimurium TA98 and TA100
bacterial strains (Reid et al. 2006).
No adverse effects of the genus have been reported in
the literature until now. Various species of the genus like C.
infortunatum, C. serratum, C. phlomidis have been reported
to be safe in the prescribed dosage in traditional system of
medicines (Anynomous 1; Sharma PV 2001).
SUMMARY
The genus Clerodendrum has been cited in many indigenous
systems of health care for the treatment of variety of disorders. A few species extensively used as folk medicines for
years have been investigated for their chemical constituents
and biological activity to confirm these traditional claims.
The genus is reported to have activities against a wide spec-
Medicinal and Aromatic Plant Science and Biotechnology 1(1), 142-150 ©2007 Global Science Books
trum of disorders which includes many life-threatening diseases like HIV. Still there are many species of the genus
having a potential towards many disorders in their unexplored fold.
clerodendron inerme L. growing in Egypt. Zagazig Journal of Pharmaceutical Science 5, 49-53
Gabriele L, Rimpler H (1981) Iridoids in Clerodendrum thomsonae Balf. F.,
Verbanaceae. Zeitschrift fur Naturforschung C: A Journal of Biosciences 36C,
708-713
Gabriele L, Rimpler H (1983) Distribution of iridoid glycosides in Clerodendrum species. Phytochemistry 22, 1729-1734
Gao LM, Wei XM, He YQ (2003a) Studies on chemical constituents in leafs of
Clerodendron fragrans. Zhongguo Zhong Yao Za Zhi 28, 948-951
Gao LM, Wei XM, He YQ (2003b) Studies on chemical constituents of Clerodendrum bungei. Zhongguo Zhong Yao Za Zhi 28, 1042-1044
Garg SC, Siddiqui N (1992) Anthelmintic activity of Vernonia teres L., and
Clerodendrum phlomidis L. Journal of Research Education in Indian Medicine 11, 1-3
Garg VP, Verma SCL (2006) Chemical examination of Clerodendron serratum: Isolation and characterization of D-mannitol. Journal of Pharmaceutical
Sciences 56, 639-640
Gayar R, Shazll A (1968) Toxicity of certain plants to Culex pipiens larvae.
Bulletin of the Society of Entomology, Egypt 52, 467
George M, Pandalai KM (1949) Investigations on plant antibiotics, Part IV.
Further search for antibiotic substances in Indian medicinal Plants. Indian
Journal of Medical Research 37, 169-181
Goswami P, Kotoky J, Chen Z-N, Lu Y (1996) A sterol glycoside from leaves
of Clerodendron colebrookianum. Phytochemistry 41, 279-281
Hazekamp A, Verpoorte R, Panthong A (2001) Isolation of a bronchodilator
flavonoid from the Thai medicinal plant Clerodendrum petasites. Journal of
Ethnopharmacology 78, 45-49
Hsiao JY, Lin ML (1995) A Chemotaxonomic study of essential oils from the
leaves of genus Clerodendrum (Verbenaceae) native to Taiwan. Botany Bulletin Academica Sinica 36, 247-251
Hsu YC, Chen C, Yuh P, Hsu HY (1983) Constituents of Clerodendron paniculatum Linn var. albiflorum Hemsl. Chung-kuo Nung Yeh Hua Hsueh Hui
Chih 21, 26
Joshi KC, Singh P, Mehra A (1979) Chemical investigation of the roots of different Clerodendron species. Planta Medica 37, 64-66
Kalyanasundaram M, Das PK (1985) Larvicidal and synergestic activity of
plant extracts for mosquito control. Indian Journal of Medical Research 82,
19-23
Kanchanapoom T, Chumsri P, Kasai R, Otsuka H, Yamasaki K (2005) A
new iridoid diglycoside from Clerodendrum chinense. Journal of Asian Natural Products Research 7, 269-272
Kanchanapoom T, Kasaia R, Chumsric P, Hiragad Y, Yamasaki K (2001)
Megastigmane and iridoid glucosides from Clerodendrum inerme. Phytochemistry 58, 333-336
Kang DG, Lee YS, Kim HJ, Lee YM, Lee HS (2003) Angiotensin converting
enzyme inhibitory phenylpropanoid glycosides from Clerodendron trichotomum. Journal of Ethnopharmacology 89, 151-154
Kawai K, Amano T, Nishida R, Kuwahara Y, Fukami H (1998) Clerodendrins from Clerodendron trichotomum and their feeding stimulant activity for
the turnip sawfly. Phytochemistry 49, 1975-1980
Khan MA, Singh VK (1996) A folklore survey of some plants of Bhopal district forest Madhya Pradesh India described as antidiabetics. Fitoterapia 67,
416-421
Kim HJ, Woo ER, Shin CG, Hwang DJ, Park H, Lee YS (2001) HIV-I integrase inhibitory phenyl propanoid glycosides from C. trichotomum. Archives
in Pharmacological Research 24, 286-291
Kirtikar KR, Basu BD (1991) Indian Medicinal Plants (2nd Edn, Vol III) Bishen Singh Mahendra Pal Sing Publication, 1945 pp
Kumar D, Verma HN, Tuteja N, Tewari KK (1997) Cloning and characterisation of a gene encoding an antiviral protein from Clerodendrum aculeatum
L. Plant Molecular Biology 33, 745-751
Kumari GNK, Balachandran J, Aravind S, Ganesh MR (2003) Antifeedant
and growth inhibitory effects of some neo-clerodane diterpenoids isolated
from Clerodendron species (Verbanaceae) on Earias vitella and Spodoptera
litura. Journal of Agriculture and Food Chemistry 51, 1555-1559
Lee JH, Lee JY, Kang HS, Jeong CH, Moon H, Whang WK, Kim CJ, Sim
SS (2006) The effect of acteoside on histamine release and arachidonic acid
release in RBL-2H3 mast cells. Archives in Pharmacological Research 29,
508-513
Lu Y-L, Fu F-Y (1980) Studies on the chemical constituents of Clerodendron
dauricum L. Part IV. Identification of carboxylic acids. Ts'ao Yao 11, 152-153
Masuda T, Yonemori S, Oyama Y, Takeda Y, Tanaka T, Andoh T, Shinohara
A, Nakata M (1999) Evaluation of the antioxidant activity of environmental
plants: activity of the leaf extracts from seashore plants. Journal of Agriculture and Food Chemistry 47, 1749-1754
Mehdi H, Tan GT, Pezzuto JM, Fong HHS, Farnsworth NR, EL-Feraly FS
(1997) Cell culture assay system for the evaluation of natural product mediated anti-hepatitis B virus activity. Phytomedicine 3, 369-377
Miller RE, McConville MJ, Woodrow IE (2006) Cyanogenic glycosides from
the rare Australian endemic rainforest tree Clerodendrum grayi (Lamiaceae).
Phytochemistry 67, 43-51
Min YS, Yim SH, Bai KL, Choi HJ, Jeong JH, Song HJ, Park SY, Ham I,
Whang WK, Sohn UD (2005) The effects of apigenin-7-O-E -D-glucuro-
ACKNOWLEDGEMENT
The authors wish to thank Mr. H. Srinivasa for his help in preparing the manuscript.
REFERENCES
Abdul-Alim MA (1971) A chemical study of the leaves of Clerodendron
inerme. Planta Medica 19, 318-321
Achari B, Chaudhuri C, Saha CR, Dutta PK, Pakrashi SC (1990) A clerodane diterpene and other constituents of Clerodendron inerme. Phytochemistry 29, 3671-3673
Achari B, Giri C, Saha CR, Dutta PK, Pakrashi SC (1992) A neo-clerodane
diterpene from Clerodendron inerme. Phytochemistry 31, 338-340
Akihisa T, Ghosh P, Thakur S, Nagata H, Tamura T, Matsumoto T (1990)
24,24-dimethyl-25-dehydrolophenol, a 4-D-methylsterol from Clerodendrum
inerme. Phytochemistry 29, 1639-1641
Akihisa T, Matsubara Y, Ghosh P, Thakur S, Tamura T, Matsumoto T
(1989) Sterols of some Clerodendrum species (Verbenaceae) occurring of the
24-D and 24-E epimers of 24-ethylsterols lacking a '25-bond. Steroids 53,
625-638
Anam EM (1997) Novel flavone and chalcone glycosides from Clerodendron
phlomidis (Verbenaceae). Indian Journal of Chemistry 36B, 897-900
Anam EM (1999) Novel flavonone and chalcone glycosides from Clerodendron phlomidis (Verbanaceae). Indian Journal of Chemistry 38B, 1307-1310
Anynomous (1992) The Useful Plants of India, Publication and Information
Directorate, CSIR, New Delhi, 132 pp
Anynomous 1 (2005) Quality Standards of Indian Medicinal Plants (Vol 3) Indian Council of Medical Research, New Delhi, 167 pp
Atta-Ur-Rehman, Begum S, Saied S, Choudhary MI, Farzana A (1997) A
steroidal glycoside from Clerodendron inerme. Phytochemistry 45, 17211722
Bashwira S, Hootelé C, Tourwé D, Pepermans H, Laus G, van Binst G
(1989) Cleromyrine I, a new cyclohexapeptide from Clerodendrum myricoides. Tetrahedron 18, 5845-5852
Bhakuni OS, Dhar ML, Dhar MM, Dhavan BN, Mehrotra BN (1969)
Screening of Indian plants for biological activities Part II. Indian Journal of
Experimental Biology 7, 250-262
Bolger LM, Rees HH, Ghisalberti EL, Goad LJ, Goodwin TW (1970) Isolation of two new sterols from Clerodendrum campbellii. Tetrahedron Letters
11, 3043-3046
Bolger LM, Rees HH, Gisalberti EL, Goad LJ, Goodwin TW (1970) Biosynthesis of 24-ethylcholesta-5, 22, 25-trien-3E-ol, a new sterol from Clerodendrum campbellii. Biochemistry Journal 118, 197-200
Calis I, Hosny M, Yuruker A (1994) Inerminosides A1, C and D three iridoid
glycosides from Clerodendron inerme. Phytochemistry 37, 1083-1085
Calis I, Hosny M, Yuruker A, Wright AD, Sticher O (1994) Inerminosides A
and B two novel complex iridoid glycosides from Clerodendron inerme.
Journal of Natural Products 57, 494-500
Chae S, Kang KA, Kim JS, Hyun JW, Kang SS (2006) Trichotomoside: A
new antioxidative phenylpropanoid glycoside from Clerodendron trichotomum. Chemistry and Biodiversity 3, 41-48
Chae S, Kim JS, Kang KA, Bu HD, Lee Y, Hyun JW, Kang SS (2004)
Antioxidant activity of jionoside D from Clerodendron trichotomum. Biological and Pharmaceutical Bulletin 27, 1504-1508
Chae S, Kim JS, Kang KA, Bu HD, Lee Y, Seo YR, Hyun JW, Kang SS
(2005) Antioxidant activity of isoacteoside from Clerodendron trichotomum.
Journal of Toxicology and Environmental Health A 68, 389-400
Chaturvedi GN, Subramaniyam PN, Tiwari SK, Singh KP (1984) Experimental and clinical studies of diabetes mellitus evaluating the efficacy of an
indigenous oral hypoglycemic drug – arani. Ancient Science Life 3, 216-224
Cheng H-H, Wang H-K, Ito J, Bastow KF, Tachibana Y, Nakanishi Y, Xu Z,
Luo T-Y, Lee K-H (2001) Cytotoxic pheophorbide-related compounds from
Clerodendrum calamitosum and C. cyrtophyllum. Journal of Natural Products 64, 915-919
Choi J-H, Wang W-K, Kim H-J (2004) Studies on the anti-inflammatory
effects of Clerodendron trichotomum thunberg leaves. Archives of Pharmacological Research 27, 189-193
Cox PA, Sperry LB, Tuominen M, Bohlin L (1989) Pharmacological activity
of the Samoan Ethnopharmacopoeia. Economic Botany 43, 487-497
Devi R, Sharma DK (2004) Hypolipidemic effect of different extracts of Clerodendron colebrookianum Walp in normal and high-fat diet fed rats. Journal
of Ethnopharmacology 90, 63-68
Dorsaz A-C, Marston A, Stoeckli-Evans H, Msonthi JD, Hostettmann K
(2004) Uncinatone, a new antifungal hydroquinone diterpenoid from Clerodendrum uncinatum Schinz. Helvetica Chimica Acta 68, 1605-1610
El-Shamy AM, El-Shabrawy ARO, El-Fiki N (1996) Phytochemical study of
149
Clerodendron and healthcare. Shrivastava and Patel
nopyranoside on reflux oesophagitis and gastritis in rats. Autonomic and Autacoid Pharmacology 25, 85-91
Misra TN, Singh SR, Pandey HS, Kohli YP (1995) Antibacterial and antifungal activity of three volatile hexane eluates extracted from the leaves of C.
colebrookianum. International Seminar on Recent Trends in Pharmaceutical
Sciences, Ootacamund, Abstract No 29
Moldenke HN (1985) Notes on the genus Clerodendrum (Verbenaceae). IV.
Phytologia 57, 334-365
Muregi FW, Chhabra SC, Njagi EN, Lang'at-Thoruwa CC, Njue WM,
Orago AS, Omar SA, Ndiege IO (2004) Anti-plasmodial activity of some
Kenyan medicinal plant extracts singly and in combination with chloroquine.
Phytotherapy Research 18, 379-384
Muregi FW, Ishih A, Miyase T, Suzuki T, Kino H, Amano T, Mkoji GM,
Terada M (2007) Antimalarial activity of methanolic extracts from plants
used in Kenyan ethnomedicine and their interactions with chloroquine (CQ)
against a CQ-tolerant rodent parasite, in mice. Journal of Ethnopharmacology 111, 190-195
Murugesan T, Saravanan KS Lakshmi S, Ramya G, Thenmozhi K (2001)
Evaluation of psychopharmacological effects of Clerodendrum phlomidis
Linn. extract. Phytomedicine 8, 472-476
Narayanan N, Thirugnanasambantham P, Viswanathan S, Vijayasekaran V,
Sukumar E (1999) Antinociceptive, anti-inflammatory and antipyretic effects of ethanol extract of Clerodendron serratum roots in experimental animals. Journal of Ethnopharmacology 65, 237-241
Nishida R, Kawai K, Amano T, Kuwahara Y (2004) Pharmacophagous
feeding stimulant activity of neo-clerodane diterpenoids for the turnip sawfly,
Athalia rosae fuficornis. Biochemistry and Systematic Ecology 32, 15-25
Nyegue MA, Belinga-Ndoye CF, Amvam Zollo PH, Agnaniet H, Menut C,
Bessière JM (2005) Aromatic plants of tropical central Africa, Part L, Volatile components of Clerodendrum buchholzii Gürke from Cameroon. Flavour
and Fragrance Journal 20, 321-323
Olivieri F, Prasad V, Valbonesi P, Srivastava S, Ghosal-Chowdhury P,
Barbieri L, Bolognesi A, Stirpe F (1996) A systemic antiviral resistance-inducing protein isolated from Clerodendrum inerme Gaertn. is a polynucleotide adenosine glycosidase (ribosome-inactivating protein). FEBS Letters
396, 132-134
Pandey R, Verma RK, Singh SC, Gupta MM (2003) 4D-methyl-24E -ethyl5D-cholesta-14,25-dien-3E-ol and 24E -ethylcholesta-5, 9(11), 22e-trien-3E ol, sterols from Clerodendrum inerme. Phytochemistry 63, 415-420
Panthong D, Kanjanapothi T, Taesotikul T, Wongcomea V (2003) Anti-inflammatory and antipyretic properties of Clerodendrum petasites S. Moorea.
Journal of Ethnopharmacology 85, 151-156
Pinto WJ, Nes WR (1985) 24ȕ-ethylsterols, n-alkanes and n-alkanols of Clerodendrum splendens. Phytochemistry 24, 1095-1097
Raha P, Banerjee H, Das AK (1989) Occurrence of three 5-hydroxyflavones
in Clerodendron scandens and Clerodendron inerme Linn. Indian Journal of
Chemistry 28B, 874
Raha P, Das AK, Adityachaudhuri N, Majumdar Pl (1991) Cleroinermin A
neo-clerodane diterpenoid from Clerodendron inerme. Phytochemistry 38,
3812-3814
Rajlakshmi D, Banerjee SK, Sood S, Maulik SK (2003) In-vitro and in-vivo
antioxidant activity of different extracts of the leaves of Clerodendron colebrookianum Walp in the rat. Journal of Pharmacy and Pharmacology 55,
1681-1686
Rani S, Ahamed N, Rajaram S, Saluja R, Thenmozhi S, Murugesan T
(1999) Anti-diarrhoeal evaluation of Clerodendrum phlomidis Linn, leaf
extract in rats. Journal of Ethnopharmacology 68, 315-319
Rao LJM, Pereira J, Gurudutt KN (1993) Neo-clerodane diterpenes from
Clerodendron inerme. Phytochemistry 34, 572-574
Reid KA, Maesa J, Maesa A, van Staden J, Kimpec ND, Mulholland DA,
Verschaeve L (2006) Evaluation of the mutagenic and antimutagenic effects
of South African plants. Journal of Ethnopharmacology 106, 44-50
Roy R, Pandey VB (1995) Flavonoids of Clerodendron phlomidis. Indian
Journal of Natural Products 11, 13-14
Roy R, Pandey VB (1994) A chalcone glycoside from Clerodendron phlomidis.
Phytochemistry 37, 1775- 1776
Roy R, Pandey VB, Singh UP, Prithiviraj B (1996) Antifungal activity of the
flavonoids from C. infortunatum roots. Fitoterapia 67, 473-74
Roy R, Singh UP, Pandey VB (1995) Antifungal activity of some naturally occurring flavonoids. Oriental Journal of Chemistry 11, 145-148
Rueda RM (1993) The genus Clerodendrum (Verbenaceae) in Mesoamerica.
Annals of the Missouri Botanical Garden 80, 870-890
Seth KK, Pandey VB, Dasgupta B (1982) Flavanoids of Clerodendron phlomidis flowers. Pharmazie 37, 74-75
Sharaf A, Aboulezz AF, Abdul-Alim MA, Goman N (1969) Pharmacological
studies on the leaves of C. inerme. Quality Plant Material Vegetation 17, 293
Sharma PV (2001) Dravyaguna-Vijnana (Vol II, Vegetable Drugs), Chaukhanbha Bharati Academy, Varanasi, pp 221, 298, 300, 523
Sharma SK, Singh VP (1979) The antifungal activity of some essential oils. Indian Drugs Pharmaceutical Industry 14, 3-6
Shi XF, Du DJ, Xie DC, Ran CQ (1993) Studies on the antitumor effect of
Clerodendrum bungei Steud or C. foetidum Bge. Zhongguo Zhong Yao Za Zhi
18, 687-690, 704
Simonsen HT, Nordskjold JB, Smitt UW, Nyman W, Palpu P, Joshi P,
Varughese G (2001) In vitro screening of Indian medicinal plants for antiplasmodial activity. Journal of Ethnopharmacology 74, 195-204
Singh P, Singhi CL (1981) Chemical investigation of Clerodendron fragrans.
Journal of the Indian Chemical Society 58, 626-627
Singh R, Prakash L (1983) Chemical examination of stems of Clerodendron
inerme (L) Gaertn. (Verbenaceae). Pharmazie 38, 565
Singh VP, Sharma SK, Khan VS (1980) Medicinal plants from Ujain district
Madhya Pradesh part II. Indian Drugs and Pharmaceutical Industry 5, 7-12
Sinha NK, Pandey VB, Dasgupta B, Higuchi R, Kawasaki T (1982) Acteoside from the flowers of Clerodendron infortunatum. Indian Journal of Chemistry 22B, 97-98
Sinha NK, Pandey VB, Shah AH, Dasgupta B (1980) Chemical constituents
of the flowers of Clerodendron infortunatum. Indian Journal of Pharmaceutical Science 42, 21
Sinha NK, Seth KK, Pandey VB, Dasgupta B, Shah AH (1981) Flavonoids
from the flowers of Clerodendron infortunatum. Planta Medica 42, 296-298
Somasundaram S, Sadique J (1986) The role of mitochondrial calcium transport during inflammation and the effect of anti-inflammatory drugs. Biochemical Medicine and Metabolic Biology 36, 220-230
Somasundram S, Sadique J (1986) Anti-hemolytic effect of flavonoidal glycosides of C. inerme: An in vitro study. Fitoterapia 57, 103-110
Steane DA, Scotland RW, Mabberley DJ, Olmstead RG (1999) Molecular
systematics of Clerodendrum (Lamiaceae): its sequences and total evidence.
American Journal of Botany 86, 98-107
Steane DA, De Kok RPJ, Olmstead RG (2004) Phylogenetic relationships between Clerodendrum (Lamiaceae) and other Ajugoid genera inferred from nuclear and chloroplast DNA sequence data. Molecular Phylogenetics and Evolution 32, 39-45
Stenzel E, Rimpler H, Hunkler D (1986) Iridoid glucosides from Clerodendrum incisum. Phytochemistry 25, 2557-2561
Surendrakumar P (1988) Anti-inflammatory activity of Lippia nodiflora, Clerodendron phlomidis and Delonix elata. Journal of Research Education Indian Medicine 7, 19-20
Sweeney AP, Wyllie SG, Shalliker RA, Markham JL (2001) Xanthine oxidase
inhibitory activity of selected Australian native plants. Journal of Ethnopharmacology 75, 273-277
Toyota M, Msonthi JD, Hostettmann K (1990) A molluscicidal and antifungal
triterpenoid saponin from the roots of Clerodendrum wildii. Phytochemistry
29, 2849-2851
Vendatham TNC, Subramanian SS, Harborne JB (1977) 4’methylscutellarein and pectolinarigenin from Clerodendron inerme. Phytochemistry 16, 294
Wong KC, Tan CH (2005) Volatile constituents of the flowers of Clerodendron
fragrans (Vent.) R. Br. Flavour and Fragrance Journal 20, 429-430
Yang H, Hou A-J, Mei S-X, Sun H-D, Che C-T (2002) Constituents of
Clerodendrum bungei. Journal of Asian Natural Products Research 4, 165169
Yang H, Jiang B, Hou A-J, Lin Z-W, Sun H-D (2000) Colebroside A, a new
diglucoside of fatty acid ester of glycerin from Clerodendrum colebrookianum. Journal of Asian Natural Product Research 2, 177-185
Yang H, Wang J, Hou A-J, Guo Y-P, Lin Z-W, Sun H-D (2000) New steroids
from Clerodendrum colebrookianum. Fitoterapia 71, 641-648
Zhou P, Pang Z, Hso HQ (1982) Studies on chemical constituents of Clerodendron bungei. Zhiwu Xaebao 24, 564-567
Zhu M, Phillipson JD, Greengrass PM, Bowery NG (1996) Chemical and
biological investigation of the root bark of Clerodendrum mandarinorum.
Planta Medica 62, 393-396
150