Abstract
Two new isopimarane diterpenoids, koratanes A and B, were identified from the rhizomes of Kaempferia koratensis Picheans., Zingiberaceae, together with thirteen known isopimarane diterpenoids. Spectroscopic evidence was used to determine the structures. CD spectra were applied to determine the absolute configuration of koratanes A. The isolated compounds were evaluated for their cytotoxicity against human HL-60 and MOLT-3 leukemia cells, T-47D breast cancer cells, and MRC-5 healthy cells. Among the isolates, compounds named koratanes A, koratanes B, boesenberrol J, saraburane B, kaempulchraol A, kaempulchraol B, kaempulchraol C, and curcumrinol A showed IC50 values ranging from 42.10 to 56.57 μM against MOLT-3 cancer cell line. Koratanes B and galangol D were the most active against HL-60 and T-47D with IC50 values of 55.62 and 79.51 μM, respectively.
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Acknowledgements
The authors are grateful to the CRI staff, P. Intachote, S. Sengsai, and B. Saimanee, for the biological activity determination. We also thank T. Jenjittikul and N. Nopporncharoenkul for their help in collecting plant material.
Funding
This work was supported in part by the Thailand Science Research and Innovation (TSRI) and Chulabhorn Research Institute (Grant Nos. 36824/4274394 and 36827/4274406), and partly by the Center of Excellence on Environmental Health and Toxicology (EHT), OPS, Ministry of Higher Education, Science, Research and Innovation.
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PK planned and performed the isolation and purification of isolated compounds. SRuchisansakun collected and identified the plant material. JB analyzed the cytotoxic activity experiment and wrote the article. ST designed and coordinated the project, provided conceptual and technical guidance for all aspects of the project, and wrote the article. CM and SRuchirawat suggested and commented on this work, as well as edited the manuscript. All authors read and approved the final manuscript.
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Kongwaen, P., Boonsombat, J., Thongnest, S. et al. Cytotoxic Isopimarane Diterpenoids from Kaempferia koratensis Rhizomes. Rev. Bras. Farmacogn. 33, 415–421 (2023). https://doi.org/10.1007/s43450-023-00359-w
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DOI: https://doi.org/10.1007/s43450-023-00359-w