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The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats

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Abstract

There are many medicinal plants in traditional medicine which are used to prevent, control, and treat anemia. One of these plants is Allium saralicum R.M. Fritsch. The purpose of our research was to investigate the effect of aqueous extract of A. saralicum leaf in the treatment of hemolytic anemia. In this study, 60 rats were used. Induction of hemolytic anemia was done by three injections of Phenylhydrazine in 50 animals. Then, the rats were divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the A. saralicum at 25, 50, 100, and 200 mg/kg concentrations. At the end of day 15 of treatment, the animals of all groups were weight and then sacrificed. The blood, liver and spleen samples were drawn immediately to analyze the hematological, biochemical and histological parameters. All groups of A. saralicum (especially AS200) significantly (p ≤ 0.05) reduced the raised concentrations of Fe, ferritin, erythropoietin, ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, and creatinine and increased the levels of body weight, HDL, total protein, albumin, WBC, lymphocyte, monocytes, platelet, RBC, Hb, PCV, MCV, MCH, and MCHC as compared to the untreated group. Also, A. saralicum at all doses prevented pathological changes in the liver and spleen. In conclusion, because of aqueous extract of A. saralicum leaf anti-anemic property, it can be used as a medical supplement or drug.

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Acknowledgements

The authors would like to thank the Kermanshah University of Medical Sciences for the financial support.

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Samaneh Goorani

  2. Department of Environmental Health, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Nabi Shariatifar

  3. Halal Research Center of IRI.FDA.MOH, Tehran, Iran

    Nabi Shariatifar

  4. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Niloofar Seydi, Akram Zangeneh, Behzad Tari & Mohammad Mahdi Zangeneh

  5. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran

    Akram Zangeneh, Rohallah Moradi & Mohammad Mahdi Zangeneh

  6. Department of Microbiology, Science and Research Branch, Islamic Azad University, Arak, Iran

    Farzaneh Nazari

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  1. Samaneh Goorani
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  2. Nabi Shariatifar
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  3. Niloofar Seydi
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  8. Mohammad Mahdi Zangeneh
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Correspondence to Mohammad Mahdi Zangeneh.

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All institutional and national guidelines for the care and use of laboratory animals were followed. All animal procedures were approved by standards of Payame Noor University of Kermanshah-Iran (No. 01/Z/G 1395/12/01) on Humane Care and Use of Laboratory Animals, in accordance with the Research Ethics Committee of the Ministry of Health and Medical Education in Iran (adopted on April 17, 2006), based on the Helsinki Protocol (Helsinki, Finland, 1975).

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Goorani, S., Shariatifar, N., Seydi, N. et al. The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Orient Pharm Exp Med 19, 403–413 (2019). https://doi.org/10.1007/s13596-019-00361-5

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