Skip to main content

Advertisement

Log in

Complete chloroplast genome sequence of Chinese larch (Larix potaninii var. chinensis), an endangered conifer endemic to China

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Larix potaninii var. chinensis, an endemic larch distributed on several peaks of Qinling Mountains, is listed in China Species Red List as an endangered species. In this study, the complete chloroplast genome (cpDNA) sequence of Larix potaninii var. chinensis was determined from Illumina pair-end sequencing data. The cpDNA is 122,492 bp in length, and contains a pair of 435 bp inverted repeat (IR) region, one large single copy (LSC) region of 65,596 bp, and one small single copy (SSC) region of 56,026 bp. The genome contains 110 genes, including 72 protein-coding genes, 4 ribosomal RNA genes and 34 transfer RNA genes. The overall A + T content of the whole genome is 61.2 %, and the corresponding values of the LSC, SSC and IR regions are 62.0, 60.3 and 62.5 %, respectively. Phylogenetic analysis based on 36 chloroplast genomes indicates that L. potaninii var. chinensis is closely related to L. decidua.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  • Doyle JJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Hahn C, Bachmann L, Chevreux B (2013) Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads—a baiting and iterative mapping approach. Nucleic Acids Res 41:e129

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang M, Duan R, Wang S, Wang Z, Fan W (2016) Species presence frequency and diversity in different patch types along an altitudinal gradient: Larix chinensis Beissn in Qinling Mountains (China). PeerJ 4:e1803

    Article  PubMed  PubMed Central  Google Scholar 

  • Lohse M, Drechsel O, Kahlau S, Bock R (2013) OrganellarGenomeDRAW-a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res 41:W575–581

    Article  PubMed  PubMed Central  Google Scholar 

  • Patel RK, Jain M (2012) NGS QC Toolkit: a toolkit for quality control of next generation sequencing data. PLoS One 7:e30619

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Swofford DL (2001) Paup*: phylogenetic analysis using parsimony (and other methods), ver. 4.0b8. Sinauer, Sunderland

  • Wu CS, Wang YN, Hsu CY, Lin CP, Chaw SM (2011) Loss of different inverted repeat copies from the chloroplast genomes of Pinaceae and Cupressophytes and influence of heterotachy on the evaluation of gymnosperm phylogeny. Genome Biol Evol 3:1284–1295

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yu XM, Zhou Q, Qian ZQ, Li S, Zhao GF (2006) Analysis of genetic diversity and population differentiation of Larix potaninii var. chinensis using microsatellite DNA. Biochem Genet 44:483–493

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31670219, 31370353, J1210063) and Natural Science Basic Research Plan in Shaanxi Province of China (2015JM3106).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhan-Lin Liu.

Additional information

Kai Han and Jianfang Li have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Han, K., Li, J., Zeng, S. et al. Complete chloroplast genome sequence of Chinese larch (Larix potaninii var. chinensis), an endangered conifer endemic to China. Conservation Genet Resour 9, 111–113 (2017). https://doi.org/10.1007/s12686-016-0633-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-016-0633-9

Keywords

Navigation