Abstract
The concentration of free and bound polyamines was studied during the somatic embryogenesis induction process in Coffea canephora explants. In the present study we show that when the induction of somatic embryogenesis in C. canephora is carried out under light conditions and in the presence of the plant growth regulator, benzylaminopurine, a cytokinin, a faster response to induction is obtained. In the darkness, the response is delayed for more than 20 days, and the number of embryos is smaller. In the absence of benzylaminopurine no embryogenic response was observed. The pronounced changes in the levels of putrescine, spermidine, and spermine, both free and bound, found in C. canephora suggest that a close correlation exists between polyamine biosynthesis and somatic embryogenesis in C. canephora during a period of cellular differentiation associated with the induction of somatic embryogenesis.
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Abbreviations
- ADC:
-
Arginine decarboxylase
- BA:
-
Benzylaminopurine
- DFMA:
-
(α-DL-difluoromethylarginine
- Kin:
-
Kinetin
- LEDs:
-
Light-emitting diodes
- NAA:
-
Naphthalene acetic acid
- Pas:
-
Polyamines
- Put:
-
Putrescine
- PEM:
-
Proembryogenic masses
- SE:
-
Somatic embryogenesis
- Spd:
-
Spermidine
- Spm:
-
Spermine
References
Zimmerman, J. L. (1993). Somatic embryogenesis: A model for early development in higher plants. Plant Cell, 5, 1411–1423.
Hoshino, T., & Cuello, J. (2006). Environmental design considerations for somatic embryogenesis. In Mujib, A. & Samaj, J (Eds.), Somatic embryogenesis (pp. 25–34). Berlin, Heidelberg: Springer.
Quiroz-Figueroa, F. R., Rojas-Herrera, R., Galaz-Avalos, R. M., & Loyola-Vargas, V. M. (2006). Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell Tissue and Organ Culture, 86, 285–301.
Robie, C. A., & Minocha, S. C. (1989). Polyamines and somatic embriogénesis in carrot. I. The effects of difluoromethylornithine and difluoromethylarginina. Plant Science, 65, 45–54.
Minocha, S. C., Papa, N. S., Khan, A. J., & Samuelsen, A. I. (1991). Polyamines and somatic embryogenesis in carrot. III. Effects pf methylglyoxal bis(guanylhydrazone). Plant and Cell Physiology, 32, 395–402.
Khan, A. J., & Minocha, S. C. (1991). Polyamines and somatic embryogenesis in carrot. II. The effects of cyclohexylammonium phosphate. Journal of Plant Physiology, 137, 446–452.
Nakagawa, R., Ogita, S., Kubo, T., & Funada, R. (2006). Effect of polyamines and l-ornithine on the development of proembryogenic masses of Cryptomeria japonica. Plant Cell Tissue and Organ Culture, 85, 229–234.
Silveira, V., Santa-Catarina, C., Tun, N. N., Scherer, G. F. E., Handro, W., Guerra, M. P., & Floh, E. I. S. (2006). Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Ktze. Plant Science, 171, 91–98.
Walden, R., Cordeiro, A., & Tiburcio, A. F. (1997). Polyamines: Small molecules triggering pathways in plant growth and development. Plant Physiology, 113, 1009–1013.
Kevers, C., Le Gal, N., Monteiro, M., Dommes, J., & Gaspar, T. (2000). Somatic embryogenesis of Panax ginseng in liquid cultures: A role for polyamines and their metabolic pathways. Plant Growth Regulation, 31, 209–214.
Minocha, R., Smith, D. R., Reeves, C., Steele, K. D., & Minocha, S. C. (1999). Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata. Physioligia Plant, 105, 155–164.
Santanen, A., & Simola, L. K. (1992). Changes in polyamines metabolism during somatic embriogénesis in Picea abies. Journal of Plant Physiology, 140, 475–480.
Yadav, J. S., & Rajam, M. V. (1997). Spatial distribution of free and conjugated polyamines in leaves of Solanum melongena L. associated with differential morphogenetic capacity: Efficient somatic embryogenesis with putrescine. Journal of Experimental Botany, 48, 1537–1545.
Monteiro, M., Kevers, C., Dommes, J., & Gasper, T. (2002). A specific role for spermidine in the initiation phase of somatic embryogenesis in Panax ginseng CA meyer. Plant Cell Tissue and Organ Culture, 68, 225–232.
Amarasinghe, V., & Carlson, J. E. (1994). Subcellular localization of polyamines in embryogenic callus of white spruce (Picea glauca). Canadian Journal of Botany, 72, 788–793.
Feirer, R. P., Mignon, G., & Litvay, J. D. (1984). Arginine decarboxylase and polyamines required for embryogenesis in the wild carrot Daucus carota. Science, 223, 1433–1435.
Feirer, R. P., Wann, S. R., & Einspahr, D. W. (1985). The effects of spermidine synthesis inhibitors on in-vitro plant development. Plant Growth Regulation, 3, 319–327.
Kaur-Sawhney, R., Shekhawat, N. S., & Galston, A. W. (1985). Polyamine levels as related to growth, differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia and Avena sativa. Plant Growth Regulation, 3, 329–337.
Hadrami, I., & D’Auzac, J. (1992). Effects of polyamines biosynthetic inhibitors on somatic embryogenesis and cellular polyamines in Hevea brasiliensis. Journal of Plant Physiology, 140, 33–36.
Cvikrová, M., Binarová, P., Cenklová, V., Eder, J., & Macháeková, I. (1999). Reinitiation of cell division and polyamine and aromatic monoamine levels in alfalfa explants during the induction of somatic embryogenesis. Physioligia Plant, 105, 330–337.
Calheiros, M. B. P., Vieira, L. G. E., & Fuentes, S. R. L. (1994). Effects of exogenous polyamines on direct somatic embryogenesis in coffee. R. Bras. Fisiol. Veg, 6, 109–114.
Shoeb, F., Yadav, J. S., Bajaj, S., & Rajam, M. V. (2001). Polyamines as biomarkers for plant regeneration capacity: Improvement of regeneration by modulation of polyamine metabolism in different genotypes of indica rice. Plant Science, 160, 1229–1235.
Verhagen, S. A., & Wann, S. R. (1989). Norway spruce somatic embryogenesis: High-frequency initiation from light-cultured mature embryos. Plant Cell Tissue and Organ Culture, 16, 103–111.
Halperin, W. (1970). Embryos from somatic plant cells. In: Padykula, H.A. (Eds.), Control mechanisms in the expression of cellular phenotypes (pp. 169–191). New York: Academic Press.
Michler, C. H., & Lineberger, R. D. (1987). Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures. Plant Cell Tissue and Organ Culture, 11, 189–207.
D’Onofrio, C., Morini, S., & Bellocchi, G. (1998). Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tissue and Organ Culture, 53, 91–98.
Bach, A., & Krol, A. (2001) Effect of light quality on somatic embryogenesis in Hyacinthus orientalis L. “Delft’s Blue” Biological Bulletin Poznan 38, 103–107.
Takanori, T., & Cuello, J. (2005). Regulating radiation quality and intensity using narrow-band LEDs for optimization of somatic embryogenesis. In Proceedings of the 2005 Annual Meeting of the American Society of Agricultural Engineers (pp. 1–12). .
Latkowska, M. J., Kvaalen, H., & Appelgren, M. (2000). Genontype dependent blue and red light inhibition of the proliferation of the embryogenic tissue or Norway spruce. In Vitro Cellular and Developmental Biology-Plant, 36, 57–60.
Kintzios, S. E., & Taravira, N. (1997). Effect of genotype and light intensity on somatic embryogenesis and plant regeneration in melon (Cucumis melo L.). Plant Breed, 116, 359–362.
Kaldenhoff, R., Henningsen, U., & Richter, G. (1994) Gene activation in suspension-cultured cells of Arabidopsis thaliana during blue-light-dependent plantlet regeneration. Planta, 195, 182–187.
Torné, J. M., Moysset, L., Claparols, I., & Simón, E. (1996). Photocontrol of somatic embryogenesis and polyamine content in Araujia sericifera petals. Physiologia Plantarum, 98, 413–418.
Torné, J. M., Moisset, L., Santos, M., & Simón, E. (2001). Effect of light quality on somatic embryogenesis in Araujia sericifera. Physiologia Plantarum, 111, 405–411.
Torné, J. M., Rodriguez, P., Manich, A., Claparols, I., & Santos, M. A. (1997). Embryogenesis induction in petals of Araujia sericifera. Plant Cell Tissue and Organ Culture, 51, 95–102.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
Quiroz-Figueroa, F. R., Fuentes-Cerda, C. F. J., Rojas-Herrera, R., & Loyola-Vargas, V. M. (2002). Histological studies on the developmental stages and differentiation of two different somatic embryogenesis systems of Coffea arabica. Plant Cell Reports, 20, 1141–1149.
Yasuda, T., Fujii, Y., & Yamaguchi, T. (1985). Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant & Cell Physiology, 26, 595–597.
Tiburcio, A. F., Kaur-Sawhney, R., & Galston, A. W. (1986). Polyamine metabolism and osmotic stress. Plant Physiology, 82, 375–378.
Flores, H. E., & Galston, A. W. (1982). Polyamines and plant stress: activation of putrescine biosynthesis by osmotic shock. Science, 217, 1259–1261.
Santana-Buzzy, N., Rojas-Herrera, R., Galaz-Avalos, R. M., Ku-Cauich, R., Mijangos-Cortés, J., Gutiérrez-Pacheco, L. C., Canto-Flick, A., Quiroz-Figueroa, F. R., & Loyola-Vargas, V. M. (2007). Advances in coffee tissue culture and its practical applications. In Vitro Cellular & Developmental Biology-Plant, 43, 507–520.
Jiménez V. M., & Thomas, C. (2006). Participation of plant hormones in determination and progression of somatic embryogenesis. In A. Mujib & J. Samaj (Eds.), Somatic embryogenesis (pp. 103–118). Berlin, Heidelberg: Springer.
Staritsky, G. (1970) Embryoid formation in callus tissues of coffee. Acta Botanica Neerlandica, 19, 509–514.
Montague, M. J., Koppenbrink, J. W., & Jaworski, E. G. (1978). Polyamine metabolism in embryogenic cells of Daucus carota I. Changes in intracellular content and rates of synthesis. Plant Physiology, 62, 430–433.
Montague, M. J., Armstrong, T. A., & Jaworski, E. G. (1979). Polyamine metabolism in embryogenic cells of Daucus carota II. Changes in arginine decarboxylase activity. Plant Physiology, 63, 341–345.
Meijer, E. G. M., & Simmonds, J. (1988). Polyamine levels in relation to growth and somatic embryogenesis in tissue cultures of Medicago sativa L. Journal of Experimental Botany, 39, 787–794.
Amarasinghe, V., Dhami, R., & Carlson, J. E. (1996). Polyamine biosynthesis during somatic embryogenesis in interior spruce (Picea glauca × Picea engelmannii complex). Plant Cell Reports, 15, 495–499.
Li, Z., & Burritt, D. J. (2003). Changes in endogenous polyamines during the formation of somatic embryos from isogenic lines of Dactylis glomerata L. with different regenerative capacities. Plant Growth Regulation, 40, 65–74.
Slocum, R. D., Kaur-Sawhney, R., & Galston, A. W. (1984). The physiology and biochemistry of polyamines in plants. Archives of Biochemistry and Biophysics, 235, 283–303.
Huang, X. L., Li, X. J., Li, Y., & Huang, L. Z. (2001). The effect of AOA on ethylene and polyamine metabolism during early phases of somatic embryogenesis in Medicago sativa. Physiologia Plantarum, 113, 424–429.
Steiner, N., Santa-Catarina, C., Silveira, V., Floh, E., & Guerra, M. (2007). Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell, Tissue and Organ Culture, 89, 55–62.
Roustan, J.-P., Latché, A., & Fallot, J. (1994). Role of ethylene on induction and expression of carrot somatic embryogenesis: Relationship with polyamine metabolism. Plant Science, 103, 223–229.
Maatar, A., & Hunault, G. (1997). Influence des régulateurs de croissance sur la teneur des tissus en polyamines libres lors de l’induction de l’embryogenèse somatique chez le fenouil (Foeniculum vulgare Miller). Comptes Rendus de l’Academie des Sciences - Series III - Sciences de la Vie, 320, 245–251.
Sánchez-Gras, M. C., & Segura, J. (1988) Morphogenesis in vitro of Sideritis angustifolia: Effects of auxins, benzyladenine and spermidine. Plant Science, 57, 151–158.
Niemi, K., Sarjala, T., Chen, X., & Haggman, H. (2007) Spermidine and the ectomycorrhizal fungus Pisolithus tinctorius synergistically induce maturation of Scots pine embryogenic cultures. Journal of Plant Physiology, 164, 629–635.
Kong, L., Attree, S. M., & Fowke, L. C. (1998). Effects of polyethylene glycol and methylglyoxal bis(guanylhydrazone) on endogenous polyamine levels and somatic embryo maturation in white spruce (Picea glauca). Plant Science, 133, 211–220.
Minocha, R., Minocha, S. C., & Long, S. (2004). Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cellular and Developmental Biology-Plant, 40, 572–580.
Pedroso, M. C., Primikirios, N., Roubelakis-Angelakis, K. A., & Pais, M. S. (1997). Free and conjugated polyamines in embryogenic and non-embryogenic leaf regions of camellia leaves before and during direct somatic embryogenesis. Physiologia Plantarum, 101, 213–219.
Blázquez, S., Piqueras, A., Serna, M. D., Casas, J. L., & Fernández, J.-A. (2004) Somatic embryogenesis in saffron: Optimisation through temporary immersion and polyamine metabolism. Acta Horticulturae, 650, 269–276.
Mengoli, M., Bagni, N., Luccarini, G., & Nuti, R. V., Serafini-Fracassini, D. (1989). Daucus carota cell cultures: Polyamines and effect of polyamine biosynthesis inhibitors in the preembryogenic phase and different embryo stages. Journal of Plant Physiology, 134, 389–394.
Acknowledgments
We are grateful to Francisco Quiroz for his help to take the pictures, Emily Wortman-Wunder for editorial assistance, and the members of Jorge Vivanco’s laboratory for helpful discussion. V.M.L.V. is recipient of a scholarship from CONACYT (75351), Mexico. This work is partially funded by CONACYT (Grant No. 61415).
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We would like to dedicate this paper to Dra. Estela Sánchez, the pioneer of the Plant Biochemistry in Mexico, on occasion of her 75th anniversary.
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De-la-Peña, C., Galaz-Ávalos, R.M. & Loyola-Vargas, V.M. Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora . Mol Biotechnol 39, 215–224 (2008). https://doi.org/10.1007/s12033-008-9037-8
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DOI: https://doi.org/10.1007/s12033-008-9037-8