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Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

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Abstract

The concentration of free and bound polyamines was studied during the somatic embryogenesis induction process in Coffea canephora explants. In the present study we show that when the induction of somatic embryogenesis in C. canephora is carried out under light conditions and in the presence of the plant growth regulator, benzylaminopurine, a cytokinin, a faster response to induction is obtained. In the darkness, the response is delayed for more than 20 days, and the number of embryos is smaller. In the absence of benzylaminopurine no embryogenic response was observed. The pronounced changes in the levels of putrescine, spermidine, and spermine, both free and bound, found in C. canephora suggest that a close correlation exists between polyamine biosynthesis and somatic embryogenesis in C. canephora during a period of cellular differentiation associated with the induction of somatic embryogenesis.

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Abbreviations

ADC:

Arginine decarboxylase

BA:

Benzylaminopurine

DFMA:

(α-DL-difluoromethylarginine

Kin:

Kinetin

LEDs:

Light-emitting diodes

NAA:

Naphthalene acetic acid

Pas:

Polyamines

Put:

Putrescine

PEM:

Proembryogenic masses

SE:

Somatic embryogenesis

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

We are grateful to Francisco Quiroz for his help to take the pictures, Emily Wortman-Wunder for editorial assistance, and the members of Jorge Vivanco’s laboratory for helpful discussion. V.M.L.V. is recipient of a scholarship from CONACYT (75351), Mexico. This work is partially funded by CONACYT (Grant No. 61415).

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Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Merida, Yucatan, Mexico

    Clelia De-la-Peña, Rosa M. Galaz-Ávalos & Víctor M. Loyola-Vargas

  2. Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO, 80523-1173, USA

    Víctor M. Loyola-Vargas

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  1. Clelia De-la-Peña
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  2. Rosa M. Galaz-Ávalos
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Correspondence to Víctor M. Loyola-Vargas.

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We would like to dedicate this paper to Dra. Estela Sánchez, the pioneer of the Plant Biochemistry in Mexico, on occasion of her 75th anniversary.

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De-la-Peña, C., Galaz-Ávalos, R.M. & Loyola-Vargas, V.M. Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora . Mol Biotechnol 39, 215–224 (2008). https://doi.org/10.1007/s12033-008-9037-8

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