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Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses

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Abstract

Alternaria brassicicola is a necrotrophic pathogenic fungus that severely affects both the yield and quality of Gladiolus cut flowers and cormels. The jasmonate signaling pathway plays an important role in plant disease resistance. The protein CORONATINE INSENSITIVE 1 (COI1) is a key regulator of this pathway, and the COI1 gene can modulate jasmonate signaling-induced defenses against necrotrophic fungi. Here, we characterize a COI1 gene from Gladiolus, GhCOI1. GhCOI1 transcript levels were greatest in the leaves, followed by the roots. A low MeJA concentration promoted GhCOI1 expression, which remained high after 6 h. Silencing GhCOI1 via VIGS reduced GhCOI1 transcript levels. The length of fungal hyphae and amount of fungal DNA were significantly greater in silenced plants than in control plants. Moreover, GhCOI1 was necessary for the induction of SOD, POD, PPO, and CAT in the Gladiolus defense response to A. brassicicola infestation. GhCOI1 silencing reduced the accumulation of H2O2, allowing the fungus to enter leaf tissue through stomata and cause necrosis directly. Taken together, these results suggest that GhCOI1 is an essential signaling component that controls the JA-regulated defense response against A. brassicicola in Gladiolus plants.

Key message

The results of this study reveal that silencing GhCOI1 reduced the disease resistance of Gladiolus

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31171991 to MFY and 31701952 to JW) and the Chinese Postdoctoral Science Foundation (2018M641569 to SSS).

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Authors and Affiliations

  1. Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Shanshan Seng

  2. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China

    Shanshan Seng, Chenyu Wu, Jian Wu, Xionghui Zhong, Junna He & Mingfang Yi

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  1. Shanshan Seng
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  2. Chenyu Wu
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  3. Jian Wu
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  4. Xionghui Zhong
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  5. Junna He
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  6. Mingfang Yi
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Contributions

SSS and CYW designed the experiments. SSS, CYW and JW performed the experiments. SSS and XHZ analyzed the data. SSS wrote the manuscript. JW, JNH and MFY revised the manuscript.

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Correspondence to Jian Wu or Junna He.

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Communicated by Degao Liu.

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Seng, S., Wu, C., Wu, J. et al. Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses. Plant Cell Tiss Organ Cult 140, 69–81 (2020). https://doi.org/10.1007/s11240-019-01711-6

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  • DOI: https://doi.org/10.1007/s11240-019-01711-6

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