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Development of SSR markers for Carex curvula (Cyperaceae) and their importance in investigating the species genetic structure

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Abstract

Background

Microsatellite primers were developed and tested to genotype several populations of Carex curvula s. l. (Cyperaceae), in order to infer the phylogeographic relationships of the populations within species and the boundaries between the two described subspecies: C. curvula subsp. curvula and C. curvula subsp. rosae.

Methods and results

Candidate microsatellite loci were isolated based on next-generation sequencing. We tested 18 markers for polymorphism and replicability in seven C. curvula s. l. populations and identified 13 polymorphic loci with dinucleotide repeats. Genotyping results showed the total number of alleles per locus varied from four to 23 (including both infrataxa), and the observed and expected heterozygosity ranged between 0.1 to 0.82 and 0.219 to 0.711, respectively. Furthermore, the NJ tree showed a clear separation between C. curvula subsp. curvula and C. curvula subsp. rosae.

Conclusion

The development of these highly polymorphic markers proved to be very efficient not only in delineating between the two subspecies, but also in genetic discriminating at population level within each infrataxon. They are promising tools for evolutionary studies in Cariceae section, as well as in providing knowledge on patterns of the species phylogeography.

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Fig. 1

Data availability

The raw data supporting the results of this article will be made available by the authors, without undue reservation.

References

  1. Puşcaş M, Choler P, Tribsch A, Gielly L, Rioux D, Gaudeul M, Taberlet P (2008) Post-glacial history of the dominant alpine sedge Carex curvula in the European Alpine System inferred from nuclear and chloroplast markers. Molec Ecol 17(10):2417–2429. https://doi.org/10.1111/j.1365-294X.2008.03751.x

    Article  CAS  Google Scholar 

  2. Pușcaș M, Choler P (2012) A biogeographic delineation of the European Alpine System based on a cluster analysis of Carex curvula-dominated grasslands. Flora 207:168–178. https://doi.org/10.1016/j.flora.2012.01.002

    Article  Google Scholar 

  3. Gilomen H (1938) Carex curvula All. sp. nov. rosae (Kalk-Krummsegge). Bericht über das Geobotanische Forschungsinstitut Rübel. 77–104.

  4. Choler P, Erschbamer B, Tribsch A, Gielly L, Taberlet P (2004) Genetic introgression as a potential to widen species’ niche: insights from alpine Carex curvula. PNAS USA 101:171–176. https://doi.org/10.1073/pnas.223723510

    Article  CAS  PubMed  Google Scholar 

  5. Chater AO (1980) Carex L. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea. Cambridge University Press, Cambridge, pp 290–323

    Google Scholar 

  6. Hodoki Y, Ohbayashi K, Kunii H (2009) Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. Populations using newly developed microsatellite markers. Conserv Genet 10:1361–1364. https://doi.org/10.1007/s10592-008-9678-y

    Article  Google Scholar 

  7. Liu W, Zhou Y, Liao H, Zhao Y, Song Z (2011) Microsatellite primers in Carex moorcroftii (Cyperaceae), a dominat species of the steppe on the Qinghai-Tibetan plateau. Am J Bot. https://doi.org/10.3732/ajb.1100105

    Article  PubMed  Google Scholar 

  8. Arroyo MJ, Escudero M, Jordano P (2016) Isolation of 91 polymorphic microsatellite loci in the western Mediterranean endemic Carex helodes (Cyperaceae). Appl Plant Sci 4(1):1500085. https://doi.org/10.3732/apps.1500085

    Article  Google Scholar 

  9. Nagasawa K, Setoguchi H, Maki M, Goto H, Fukushima K, Isagi Y, Sakaguchi S (2018) Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields. Appl Plant Sci 6(10):e1185. https://doi.org/10.1002/aps3.1185

    Article  Google Scholar 

  10. Liu L, Fan X, Tan P, Wu J, Zhang H, Han C, Chen C, Xun L, Guo W, Chang Z, Teng K (2021) The development of SSR markers based on RNA-sequencing and its validation between and within Carex L. species. BMC Plant Biol 21(1):1–15. https://doi.org/10.1186/s12870-020-02792-8

    Article  CAS  Google Scholar 

  11. Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x

    Article  Google Scholar 

  12. Takezaki N, Nei M, Tamura K (2010) POPTREE2: software for constructing population trees from allele frequency data and computing other population statistics with Windows interface. Mol Biol Evol 27(4):747–752. https://doi.org/10.1093/molbev/msp312

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This research was supported by the ODYSSEE project (ANR-13-ISV7-0004, PN-II-ID-JRP-RO-FR-2012) and BioDivMount project (BRANCUSI No. 32660WB and PN-II-CT-ROFR-2014-2-0011), funded by ANR France and UEFISCDI Romania (coordinators Philippe Choler and Mihai Pușcaș). Dana Șuteu and Ioan Băcilă received funding from the core project BIORESGREEN, subproject BioClimpact no. 7/30.12.2022, code 23020401. The authors thank Anna and Michał Ronikier for help with field sampling. The authors extend their gratitude to the anonymous reviewer for his constructive feedback.

Funding

This research was supported by the ODYSSEE project (ANR-13-ISV7-0004, PN-II-ID-JRP-RO-FR-2012) and BioDivMount project (BRANCUSI No. 32660WB and PN-II-CT-ROFR-2014-2-0011), funded by ANR France and UEFISCDI Romania (coordinators Philippe Choler and Mihai Pușcaș). Dana Șuteu and Ioan Băcilă received funding from the core project BIORESGREEN, subproject BioClimpact no. 7/30.12.2022, code 23020401.

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Authors and Affiliations

  1. Department of Experimental Biology, National Institute of Research and Development for Biological Sciences, 48 Republicii Street, 400015, Cluj-Napoca, Romania

    Dana Șuteu, Ioan Băcilă & Mihai Miclăuș

  2. A. Borza Botanic Garden, Babeș-Bolyai University, 42 Republicii Street, 400015, Cluj-Napoca, Romania

    Mihai Pușcaș

  3. Faculty of Biology and Geology, Babeș-Bolyai University, 44 Republicii Street, Cluj-Napoca, Romania

    Mihai Pușcaș & Zoltán Robert Balázs

  4. Center for Systematic Biology, Biodiversity and Bioresources—3B, Faculty of Biology and Geology, Babeș-Bolyai University, 1 Kogălniceanu Street, 400084, Cluj-Napoca, Romania

    Mihai Pușcaș & Zoltán Robert Balázs

  5. Emil G. Racoviță Institute, Babeș-Bolyai University, Cluj-Napoca, Romania

    Mihai Pușcaș

  6. Babeș-Bolyai University, 1 Kogălniceanu Street, 400084, Cluj-Napoca, Romania

    Mihai Miclăuș

  7. Doctoral School of Integrative Biology, Babeș-Bolyai University, 1 Kogălniceanu Street, 400084, Cluj-Napoca, Romania

    Zoltán Robert Balázs

  8. University Grenoble Alpes, University Savoie Mont Blanc, CNRS, LECA, 38000, Grenoble, France

    Philippe Choler

Authors
  1. Dana Șuteu
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  2. Mihai Pușcaș
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  3. Ioan Băcilă
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  4. Mihai Miclăuș
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  5. Zoltán Robert Balázs
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  6. Philippe Choler
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Contributions

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.

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Correspondence to Mihai Pușcaș.

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Șuteu, D., Pușcaș, M., Băcilă, I. et al. Development of SSR markers for Carex curvula (Cyperaceae) and their importance in investigating the species genetic structure. Mol Biol Rep 50, 4729–4733 (2023). https://doi.org/10.1007/s11033-023-08362-z

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  • DOI: https://doi.org/10.1007/s11033-023-08362-z

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