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Allelopathy on Bark of Downed Logs of Chamaecyparis Obtusa sieb. and Zucc. var. formosana (Hayata) Rehder

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Abstract

Chamaecyparis obtusa Sieb. and Zucc. var. formosana (Hayata) Rehder is the dominant species in the temperate forest of Yuanyang Lake Nature Reserve (YYL), Taiwan. Although downed logs of C. obstusa var. formosana occupy only a small percentage of the forest floor area in YYL, they are important regeneration substrates. Seedlings of this species often grow without competition on the new downed logs, and a few broadleaf trees grow with them. We hypothesized that the bark of the newly fallen logs possesses allelopathic potential that provides a habitat especially suitable for seedling establishment. Eight different seeds including those from Lactuca sativa L. (lettuce), Bidens pilosa (an invasive weed), and six species in YYL were planted on the bark of the downed logs in an incubator for germination tests. Two dominant species in the forest of YYL, C. obtusa var. formosana and Rhododendron formosanum, were able to grow normally, but the others, Pieris taiwanensis, Barthea formosana, Chamaecyparis formosensis, Miscanthus transmorrisonensis, lettuce, and B. pilosa were growth inhibited. A bioactivity-guided isolation was designed to isolate allelochemicals from the bark. Salicylic acid, one of the inhibiting substances, was isolated and identified by gas chromatography/mass spectroscopy (GC/MS), proton nuclear magnetic resonance (1H NMR), and infrared (IR). Bioassay of salicylic acid confirmed a phytotoxic effect. The results suggest that the dominance of C. obtusa var. formosana seedlings on bark could be partly due to allelopathy.

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References

  • An, M., Pratley, J. E., and Haig, T. 2001. Phytotoxicity of Vulpia residues: III. Biological activity of identified allelochemicals from Vulpia myuros. J. Chem. Ecol. 27:383–394.

    Article  PubMed  CAS  Google Scholar 

  • Blum, U., Shafer, S. R., and Lehman, M. E. 1999. Evidence for inhibitory allelopathic interactions involving phenolic acids in field soils: concepts vs. an experimental model. Crit. Rev. Plant Sci. 18:673–693.

    Article  CAS  Google Scholar 

  • Bonanomi, G., Sicurezza, M. G., Caporaso, S., Esposito, A., and Mazzoleni S. 2006. Phytotoxicity dynamics of decaying plant materials. New Phytol. 169:571–578.

    Article  PubMed  CAS  Google Scholar 

  • Brang, P., Moran, J., Puttonen, P., and Vyse, A. 2003. Regeneration of Picea engelmannii and Abies lasiocarpa in high-elevation forests of south-central British Columbia depends on nurse logs. For. Chron. 79:273–279.

    Google Scholar 

  • Chang, N. H., Hsui, Y. R., Horng, F. W., Yu, H. M., and Ma, F. C. 2001. Natural seeding and seedling occurrence in the Chamaecyparis forest at Chilan Mt. area. Taiwan J. For. Sci. 16:321–326.

    Google Scholar 

  • Chen, J. S. and Chiu, C. Y. 2000. Effect of topography on the composition of soil organic substances in a perhumid subtropical montane forest ecosystem in Taiwan. Geoderma. 96:19–30.

    Article  CAS  Google Scholar 

  • Chen, S. H. and Wu, J. T. 1999. Paleolimnological environment indicated by the diatom and pollen assemblages in an alpine lake of Taiwan. J. Paleolimnol. 22:149–158.

    Article  Google Scholar 

  • Chen, S. H. and Yang, Y. L. 1996. Study of vegetation history around Yuan-Yang Lake. The Joint Sym. On Taiwan Quaternary (6) and on Investigation of Subsurface Geology and Engineering Environment of Taipei Basin, pp. 257–260.

  • Cheng, D. and Igarashi, T. 1987. Fungi associated with natural regeneration of Picea jezoensis Carr. in seed stage—their distribution on forest floors and pathogenicity to the seeds. Res. Bull. Coll. Exp. For. Fac. Agric. Hokkaido Univ. 44:175–188.

    Google Scholar 

  • Chiu, C. M., Lo-Cho, C. N., and Chung, H. H. 1993. The cypress stand of natural regeneration sites in Chi-lan-shan area. Bull. Taiwan For. Res. Inst. 8:389–402.

    Google Scholar 

  • Chiu, C. M., Lo-Cho, C. N., and Chung, H. H. 1995. The stem form and crown structure of natural regeneration stands of Chamaecyparis taiwanensis in Chi-Lan-Shan Area. Bull. Taiwan For. Res. Inst. 10:121–130.

    Google Scholar 

  • Chiu, C. Y., Lai, S. Y., Lin, Y. M., and Chiang, H. C. 1999. Distribution of the radionuclide 137Cs in the soils of a wet mountainous forest in Taiwan. Appl. Radiat. Isotopes 50:1097–1103.

    Article  CAS  Google Scholar 

  • Chou, C. H., Chen T. Y., Liao C. C., and Peng C. L. 2000. Long-term ecological research in the Yuanyang Lake forest ecosystem I. Vegetation composition and analysis. Bot. Bull. Acad. Sin. 41:61–72.

    Google Scholar 

  • Chou, C. H. and Patrick, Z. A. 1976. Identification and phytotoxic activity of compounds produced during decomposition of corn and rye residues in soil. J. Chem. Ecol. 2:369–387.

    Article  CAS  Google Scholar 

  • Christy, E. J. and Mack, R. N. 1984. Variation in the demography of juvenile Tsuga heterophylla across the substratum mosaic. J. Ecol. 72:75–91.

    Article  Google Scholar 

  • Chung, I. M., Ahn, J. K., and Yun, S. J. 2001. Identification of allelopathic compounds from rice (Oryza sativa L.) straw and their biological activity. Can. J. Plant Sci. 81:815–819.

    CAS  Google Scholar 

  • Chung, I. M., Seigler, D., Miller, D. A., and Kyung, S. H. 2000. Autotoxic compounds from fresh alfalfa leaf extracts: identification and biological activity. J. Chem. Ecol. 26:315–327.

    Article  CAS  Google Scholar 

  • Del Moral, R. and Cates, R. G. 1971. Allelopathic potential of the dominant vegetation of western Washington. Ecol. 52:1030–1037.

    Article  Google Scholar 

  • Emmler, T., Gieschler, S., Limbach, H. H., and Buntkowsky, G. 2004. A simple method for the characterization of OHO-hydrogen-bonds by 1H-solid state NMR spectroscopy. J. Mol. Struct. 700:29–38.

    Article  CAS  Google Scholar 

  • Guan, L. and Scandalios, J. G. 1995. Developmentally related responses maize catalase genes to salicylic acid. Proc. Natl. Acad. Sci. U S A. 92:5930–5934.

    Article  PubMed  CAS  Google Scholar 

  • Harcombe, P. A. 1987 Tree life tables. Simple birth, growth, and death data encapsulate life histories and ecological roles. BioSci. 37:557–568.

    Article  Google Scholar 

  • Harmon, M. E., Frankling, J. F., Swanson, F. J., Sollins, P., Gregory, S. V., Lattin, J. D., Anderson, N. H., Cline, S. P., Aumen, N. G., Sedell, J. R., Lienkaemper, G. W., Cromack, K. J., and Cummins, K. W. 1986. Ecology of coarse woody debris in temperate ecosystems. Adv. Ecol. Res. 15:133–302.

    Article  Google Scholar 

  • Harper, J. L. 1977. Population Biology of Plants. Academic Press, New York, NY. 892 pp.

    Google Scholar 

  • Harper, J. R. and Balke, N. E. 1981. Characterization of the inhibition of K+ absorption in oat roots by salicylic acid. Plant Physiol. 68:1349–1353.

    Article  PubMed  CAS  Google Scholar 

  • Hiura, T., Sano, T., and Konno, Y. 1996. Age structure and response to fine scale disturbances of Abies sachalinensis, Picea Jezoensis, Picea glehnii, and Betula ermanii growing under the influence of a dwarf bamboo understory in northern Japan. Can. J. For. Res. 26:289–298.

    Google Scholar 

  • Hwang, Y. H., Fan, C. W., and Yin, M. H. 1996. Primary production and chemical composition of emergent aquatic macrophytes, Schoenoplectus mucronatus ssp. robustus and Sparganium fallax, in Lake Yuanyang, Taiwan. Bot. Bull. Acad. Sin. 37:265–273.

    Google Scholar 

  • Koopmann, R. 2005. Allelopathic effects of bark phenols on epiphytic lichens. PhD dissertation, University of Bonn, Germany.

  • Lampert, H., Mikenda, W., Karpfen, A., and Kählig, H. 1997. NMR shieldings in Benzoyl and 2-hydroxybenzoyl compounds. Experimental versus GIAO calculated data. J. Phys. Chem. A 101:9610–9617.

    Article  CAS  Google Scholar 

  • Liao, C. C., Chou, C. H., and Wu, J. T. 2003. Regeneration patterns of yellow cypress on downed logs in mixed coniferous-broadleaf forest of Yuanyang Lake Nature Preserve, Taiwan. Bot. Bull. Acad. Sin. 44:229–238.

    Google Scholar 

  • Lo-Cho, C. N., Chung, H. H., Chiu, C. M., Chou, C. F., and Lo, S. S. 1989. Natural regeneration of red cypress old growth by selective cutting and intensive site preparation. Bull. Taiwan For. Res. Ins. New series 4:197–217.

    Google Scholar 

  • Lo-Cho, C. N., Chiu, C. M. and Chen, Y. C. 1999. Effects of cleaning and pruning on natural-regenerated cypress stands. Taiwan J. Sci. 14:315–321.

    Google Scholar 

  • Lusk, C. H. 1995. Seed size, establishment sites and species coexistence in a Chilean rain forest. J. Veg. Sci. 6:249–256.

    Article  Google Scholar 

  • Manthe, B., Schulz, M., and Schnabl, H. 1992. Effects of salicylic acid on growth and stomatal movements of Vicia faba L.: evidence for salicylic acid metabolization. J. Chem. Ecol. 18:1523–1539.

    Article  Google Scholar 

  • Mckee, A., Laroi, G., and Franklin, J. F. 1982. Structure, composition and reproductive behaviour of terrace forests, South Fork Hoh River, Olympic National Park. pp 22–29 in E. E. Starkey, J. F. Franklin, and J. W. Matthews, editors. Ecological research in the national parks of the Pacific Northwest. Oregon State University. Forest Research Laboratory. Corvallis, Oregon, USA.

  • MEtraux, J. P., Signer, H., Ryals, J., Ward, E., Wyss-Benz, M., Gaudin, J., Raschdorf, K., Schmid, E., Blum, W., and Inverardi, B. 1990. Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science 250:1004–1006.

    Article  CAS  PubMed  Google Scholar 

  • Moharekar, S. T., Lokhande, S. D., Hara, T., Tanaka, R., Tanaka, A., and Chavan, P. D. 2003. Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica 41:315–317.

    Article  CAS  Google Scholar 

  • Narukawa, Y., Iida, S., Tanouchi, H., Abe, S., and Yamamoto, S. I. 2003. State of fallen logs and the occurrence of conifer seedlings and saplings in boreal and subalpine old-growth forests in Japan. Ecol. Res. 18:267–277.

    Article  Google Scholar 

  • O’hanlon-Manners, D. L. and Kotanen, P. M. 2004. Logs as refuges from fungal pathogens for seeds of eastern hemlock (Tsuga Canadensis). Ecol. 85:284–289.

    Google Scholar 

  • Pancheva, T. V., Popova, L. P., and Uzunova, A. N. 1996. Effects of salicylic acid on growth and photosynthesis in barley plants. J. Plant Physiol. 149:57–63.

    CAS  Google Scholar 

  • Park, M. H., Han, M. S., Ahn, C. Y., Kim, H. S., Yoon, B. D., and Oh, H. M. 2006. Growth inhibition of bloom-forming cyanobacterium Microcystis aeruginosa by rice straw extract. Lett. Appl. Microbiol. 43:307–312.

    Article  PubMed  CAS  Google Scholar 

  • Place, I. C. M. 1955. The influence of seedbed conditions on the regeneration of spruce and balsam fir. Can. For. Br. Bull. 117. 87p.

    Google Scholar 

  • Pramanik, M. H. R., Nagai, M., Asao, T., and Matsui, Y. 2000. Effects of temperature and photoperiod on phytotoxic root exudates of cucumber (Cucumis sativus) in hydroponic culture. J. Chem. Ecol. 26:1953–1967.

    Article  CAS  Google Scholar 

  • Rajjou, L., Belghazi, M., Huguet, R., Robin, C., Moreau, A., Job, C., and Job, D. 2006. Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiol. 141:910–923.

    Article  PubMed  CAS  Google Scholar 

  • Rao, M. V., Paliyath, G., Ormrod, D. P., Murr, D. P., and Watkins C. B. 1997. Influence on salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes: salicylic acid-mediated oxidative damage requires H2O2. Plant Physiol. 115:137–149.

    Article  PubMed  CAS  Google Scholar 

  • Ridenour, W. M., and Callaway, R. M. 2001. The relative importance of allelopathy in interference: the effect of an invasive weed on a native bunchgrass. Oecologia 126:444–450.

    Article  Google Scholar 

  • Santiago, L. S. 2000. Use of coarse woody debris by the plant community of a Hawaiian montane cloud forest. Biotropica 32:633–641.

    Article  Google Scholar 

  • Sas. 2004. SAS 9.1.3 Help and Documentation. SAS Institute Inc., Cray, N. C.

  • Seal, A. N., Pratley, J. E., Haig, T., and An, M. 2004. Identification and quantitation of compounds in a series of allelopathic and non-allelopathic rice root exudates. J. Chem. Ecol. 30:1647–1662.

    Article  PubMed  CAS  Google Scholar 

  • Singh, A., Singh, A. K., and Mehrotra N. K. 2004. Correlation of the mutual viscosity and the NMR spin-lattice relaxation time in several substituted benzoic acids. Bull. Chem. Soc. Jpn. 77:661–665.

    Article  CAS  Google Scholar 

  • Singh, M., Tamma R. V., and Nigg, H. N. 1989. HPLC identification of. allelopathic compounds from Lantana camara. J. Chem. Ecol. 15:81–89.

    Article  CAS  Google Scholar 

  • Spector, T. 1978. Refinement of the Coomassie Blue method of protein quantitation. Anal. Biochem. 86:142–146.

    Article  PubMed  CAS  Google Scholar 

  • Takahashi, L., Sakai, Y., Ootomo, R., and Shiozaki, M. 2000. Establishment of tree seedlings and water-soluble nutrients in coarse woody debris in an old-growth Picea-Abies forest in Hokkaido, northern Japan. Can. J. For. Res. 30:1148–1155.

    Article  Google Scholar 

  • Tseng M. H., Kuo, Y. S., Chen, Y. M., and Chou C. H. 2003. Allelopathic potential of Macaranga tanarius (L.) Muell.-ARG. J. Chem. Ecol. 29:1269–1286.

    Article  PubMed  CAS  Google Scholar 

  • Tubbs, C. H. 1996. Aspects of eastern hemlock silvics important in silviculture: an overview. pp 5–9 in G. Mroz and J. Martin, editors. Hemlock ecology and management. Proceedings of the Regional Conference on Ecology and Management of Eastern Hemlock; 1995 September 27–28; Iron Mountain, MI. Department of Forestry, School of Natural Resources, University of Wisconsin-Madison, Wisconsin, U. S. A.

  • Wintermans, J. F. and De Mots, A. 1965. Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol. Biochem. Biophys. Acta 109:448–453.

    Article  PubMed  CAS  Google Scholar 

  • Wu, J. T., Chang, S. C., Wang, Y.S., Wang, Y. F., and Hsu, M. K. 2001. Characteristics of the acidic environment of the Yuanyang Lake (Taiwan). Bot. Bull. Acad Sin. 42:17–22.

    Google Scholar 

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Acknowledgments

The authors express their appreciation to the Forest Conservation and Management Administration, Veterans Affairs Commission, Executive Yuan, Taiwan, for permission to do this study in Yuan-yang Lake Nature Preserve and to Dr. T. H. Wang, Institute of Plant and Microbial Biology, Academia Sinica for laboratory assistance. This study was supported by Grants NSC-92-2313-B133-001 to Y. H. Kuo and M. W. Tseng.

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Authors and Affiliations

  1. Department of Science, Taipei Municipal University of Education, Taipei, 100, Taiwan, Republic of China

    Mei-Hwei Tseng, Wen-Rong Lai & Chin-Lin Hsieh

  2. Department of Chemistry, National Taiwan University, Taipei, 100, Taiwan

    Yueh-Hsiung Kuo

  3. College of Pharmacy, China Medical University, Taichung, Taiwan, 404, Republic of China

    Yueh-Hsiung Kuo

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  1. Mei-Hwei Tseng
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Tseng, MH., Lai, WR., Hsieh, CL. et al. Allelopathy on Bark of Downed Logs of Chamaecyparis Obtusa sieb. and Zucc. var. formosana (Hayata) Rehder. J Chem Ecol 33, 1283–1296 (2007). https://doi.org/10.1007/s10886-007-9278-1

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