Abstract
Phaedranassa schizantha is a species endemic to Ecuador from which eight polymorphic microsatellite loci were isolated from an enriched genomic library. A total of 31 alleles with an average of four alleles per locus were detected across 29 individuals from a single natural population of P. schizantha. Observed heterozygosity ranged from 0.05 to 0.68. Most of the eight loci were successfully amplified in six Phaedranassa species. Five of those species are either “Vulnerable” or “Endangered” under IUCN criteria. These loci will be used to investigate patterns of inter- and intraspecific variation of Phaedranassa species, which will contribute data relevant to their conservation status.
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Acknowledgments
The authors gratefully acknowledge financial support from NSF Grant DEB 0129179 to AWM. We also want to acknowledge Dr. David Kuhn, Kyoko Nakamura and plant science technicians at USDA-ARS Miami for methodological assistance. We also thank to Herbario QCA and Ministerio del Medio Ambiente of Ecuador for collection and export permits. This is contribution 161 to the Tropical Biology Program of Florida International University.
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Oleas, N.H., Meerow, A.W. & Francisco-Ortega, J. Eight microsatellite loci in Phaedranassa schizantha Baker (Amaryllidaceae) and cross-amplification in other Phaedranassa species. Conserv Genet 10, 1887–1889 (2009). https://doi.org/10.1007/s10592-009-9846-8
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DOI: https://doi.org/10.1007/s10592-009-9846-8