Abstract
To understand the speciation process of Salvia isensis (Lamiaceae), a species endemic to a special environment (serpentine areas in the Ise-Tokai district, central Honshu, Japan), chloroplast DNA (cpDNA) and nuclear ribosomal DNA (nrDNA) were employed to analyze the phylogenetic relationships of S. isensis with related species in Japan. Allozymic polymorphisms were also used to analyze genetic relationships among Salvia species. A contradiction in the phylogenetic positions of species studied was detected when phylogenetic trees were constructed using cpDNA or nrDNA, i.e., S. isensis was a sister to the other species in phylogenetic trees generated from cpDNA, while S. japonica was a sister to the other species in the case of nrDNA. Genetic relationships between Salvia species estimated from allozymic polymorphisms did not contradict to the topology for nrDNA. Using the present results, the speciation process of S. isensis is discussed with regard to introgressive gene exchanges between related species.
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Acknowledgments
We wish to express our gratitude to Dr. M.N. Tamura (Botanical Gardens, Osaka City University) and Dr. J. Yamashita (Okayama University) for comments on this manuscript, and to Prof. Dr. H. Tsukaya (University of Tokyo), Dr. G. Seto (Osaka Museum of Natural History), Ms. S. Onoue (Kashiwara city), and Mr. M. Horiuchi (Katano city) for their help in obtaining materials, to Prof. Dr. M. Kato (University of Tokyo) for permission to use facilities, to Dr. Y. Kita (University of Tokyo) for technical help, and to Mr. H. Yamada for his technical support using PAUP*. We also acknowledge anonymous reviewers for valuable comments to improve this manuscript.
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Sudarmono, Okada, H. Speciation process of Salvia isensis (Lamiaceae), a species endemic to serpentine areas in the Ise-Tokai district, Japan, from the viewpoint of the contradictory phylogenetic trees generated from chloroplast and nuclear DNA. J Plant Res 120, 483–490 (2007). https://doi.org/10.1007/s10265-007-0075-2
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DOI: https://doi.org/10.1007/s10265-007-0075-2