Smilax L. species, popularly known in Brazil as salsaparrilha, have been used in folk medicine as tonic against rheumatism and as anti-syphilitic since the sixteenth century; however, until today, their roots have been explored in an extractive way. The aim of this study was to analyze the propagation of Smilax fluminensis aiming to meet the demand and to propose procedures for its sustainable management. It was analyzed the percentage of seed germination, rooting of aerial and subterranean stem cuttings and plant resprouting capacity after removal of the shoots. The monitoring lasted one year. Seeds were subjected to temperatures: 25°C, 30°C, 20- 30°C and 20-35°C under a daily photoperiod of eight hours and in the absence of light. For rooting the stem cuttings, aerial and subterranean stems about 20cm long with two nodal regions were subjected to treatment with distilled water (control) and indolbutyric acid (IBA) at 100ppm. The best germination percentages obtained for S. fluminensis were 80% at 20-30°C under light and 85% at 30°C in the dark. Only subterranean stem cuttings showed significant difference concerning fresh and dry matter of roots with higher values in treatments with hormone compared to the control. Key words: cuttings, greenbrier, resprouting, seed germination As especies do genero Smilax L., conhecidas popularmente como salsaparrilha, sao empregadas na medicina popular como fortificante contra o reumatismo e antissifilitico desde o seculo XVI, porem ate hoje as suas raizes sao exploradas de maneira extrativista. O objetivo deste estudo foi analisar a propagacao de Smilax fluminensis visando a auxiliar no atendimento da demanda e apontar propostas para o seu manejo sustentavel. Foram analisados a porcentagem de germinacao de sementes, o enraizamento de estacas de ramos aereos e subterraneos e o acompanhamento ao longo de um ano da capacidade de rebrotamento das plantas apos a retirada dos ramos aereos. As sementes foram submetidas as temperaturas: 25, 30, 20-30 e 20-35°C, sob fotoperiodo diario de oito horas e na ausencia de luz. Para o enraizamento de estacas, foram utilizados ramos aereos e subterraneos com aproximadamente 20cm com duas regioes nodais submetidas ao tratamento com agua destilada (controle) e com acido indolbutirico (AIB) a 100ppm. As melhores porcentagens de germinacao obtidas para S. fluminensis, 80% em 20-30°C no claro e 85% a 30°C no escuro. Apenas as estacas de ramos subterraneos enraizaram, houve diferenca significativa em relacao a massa fresca e seca de raizes com valores maiores nos tratamentos com hormonio em relacao ao controle. Palavras-chave: estacas, salsaparrilha, rebrotamento, germinacao de sementes