US20060063192A1 - Nucleic acid sequencing by Raman monitoring of molecular deconstruction - Google Patents
Nucleic acid sequencing by Raman monitoring of molecular deconstruction Download PDFInfo
- Publication number
- US20060063192A1 US20060063192A1 US11/255,386 US25538605A US2006063192A1 US 20060063192 A1 US20060063192 A1 US 20060063192A1 US 25538605 A US25538605 A US 25538605A US 2006063192 A1 US2006063192 A1 US 2006063192A1
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- Prior art keywords
- nucleic acid
- nucleotides
- acid molecule
- raman
- reaction chamber
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- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/319—Exonuclease
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/632—Detection means characterised by use of a special device being a surface enhanced, e.g. resonance, Raman spectrometer
Definitions
- the present methods, compositions and apparatus relate to the fields of molecular biology and genomics. More particularly, the methods, compositions and apparatus concern nucleic acid sequencing.
- DNA deoxyribonucleic acid
- RNA ribonucleic acid
- Genetic information is stored in the form of very long molecules of DNA organized into chromosomes.
- the twenty-three pairs of chromosomes in the human genome contain approximately three billion bases of DNA sequence. This DNA sequence information determines multiple characteristics of each individual, such as height, eye color and ethnicity.
- Many common diseases, such as cancer, cystic fibrosis, sickle cell anemia and muscular dystrophy are based at least in part on variations in DNA sequence.
- RNA an intermediary molecule required for processing of genetic information, can also be sequenced in some cases to identify the genetic bases of various diseases.
- nucleic acid sequencing based on detection of fluorescently labeled nucleic acids that have been separated by size, are limited by the length of the nucleic acid that can be sequenced. Typically, only 500 to 1,000 bases of nucleic acid sequence can be determined at one time. This is much shorter than the length of the functional unit of DNA, referred to as a gene, which can be tens or even hundreds of thousands of bases in length.
- determination of a complete gene sequence requires that many copies of the gene be produced, cut into overlapping fragments and sequenced, after which the overlapping DNA sequences may be assembled into the complete gene. This process is laborious, expensive, inefficient and time-consuming.
- FIG. 1 illustrates an exemplary apparatus (not to scale) and method for DNA sequencing in which the released nucleotides are spatially separated from the nucleic acid molecule to be sequenced.
- FIG. 2 illustrates an exemplary apparatus (not to scale) and method for DNA sequencing in which the released nucleotides are not spatially separated from the nucleic acid molecule.
- the detector quantifies the nucleotides present in solution.
- the disclosed methods, compositions and apparatus are of use for the rapid, automated sequencing of nucleic acids.
- the methods, compositions and apparatus are suitable for obtaining the sequences of very long nucleic acid molecules of greater than 1,000, greater than 2,000, greater than 5,000, greater than 10,000, greater than 20,000, greater than 50,000, greater than 100,000 or even more bases in length.
- sequence information may be obtained during the course of a single sequencing run, using one molecule of nucleic acid 13 , 102 .
- multiple copies of the nucleic acid molecule 13 , 102 may be sequenced in parallel or sequentially to confirm the nucleic acid sequence or to obtain complete sequence data.
- both the nucleic acid molecule 13 , 102 and its complementary strand may be sequenced to confirm the accuracy of the sequence information.
- Advantages over prior methods of nucleic acid sequencing include the ability to read long nucleic acid sequences in a single sequencing run, greater speed of obtaining sequence data, decreased cost of sequencing and greater efficiency in terms of the amount of operator time required per unit of sequence data generated.
- the nucleic acid 13 , 102 to be sequenced is DNA, although it is contemplated that other nucleic acids 13 , 102 comprising RNA or synthetic nucleotide analogs could be sequenced as well.
- the following detailed description contains numerous specific details in order to provide a more thorough understanding of the disclosed embodiments. However, it will be apparent to those skilled in the art that the embodiments may be practiced without these specific details. In other instances, devices, methods, procedures, and individual components that are well known in the art have not been described in detail herein.
- the methods involve sequencing of individual single-stranded nucleic acid molecules 13 , 102 that are attached to an immobilization surface 14 , 103 in a reaction chamber 11 , 101 and disassembled in a deconstruction reaction.
- the reaction chamber 11 , 101 contains one or more deconstruction reagents 15 , 106 that sequentially remove one nucleotide 16 , 104 at a time from the unattached end 17 , 105 of the nucleic acid molecule 13 , 102 .
- deconstruction reagents 15 , 106 include any exonuclease known in the art.
- the nucleotides 16 , 104 are identified by Raman spectroscopy as they are released into solution.
- FIG. 1 shows an apparatus 10 for nucleic acid sequencing comprising a reaction chamber 11 attached to a flow path 12 .
- the reaction chamber 11 contains a nucleic acid molecule 13 attached to an immobilization surface 14 along with a deconstruction reagent 15 , such as an exonuclease.
- the exonuclease 15 catalyzes the sequential release of individual nucleotides 16 from the free end 17 of the nucleic acid molecule 13 .
- the detection unit 18 comprises an excitation source 19 , such as a laser, that emits an excitatory beam 20 .
- the excitatory beam 20 interacts with the released nucleotides 16 so that electrons are excited to a higher energy state.
- the Raman emission spectrum that results from the return of the electrons to a lower energy state is detected by a Raman spectroscopic detector 21 , such as a spectrometer or a monochromator.
- the released nucleotides 16 are spatially separated from the nucleic acid molecule 13 before detection by the detection unit 18 . Spatial separation acts to increase the signal-to-noise ratio of the Raman detector 21 by isolating the individual nucleotides 16 .
- FIG. 1 illustrates embodiments in which a single nucleic acid molecule 13 is contained in a single reaction chamber 11 .
- multiple nucleic acid molecules 13 may be sequenced simultaneously.
- the nucleic acid molecule 13 in each reaction chamber 11 may be identical or may be different.
- two or more nucleic acid molecules 13 may be present in a single reaction chamber 11 .
- the nucleic acid molecules 13 will be identical in sequence. Where more than one nucleic acid molecule 13 is present in the reaction chamber 11 , the Raman emission signals will represent an average of the nucleotides 16 released simultaneously from all nucleic acid molecules 13 in the reaction chamber 11 .
- the skilled artisan will be able to correct the signal obtained at any given time for deconstruction reactions that either lag behind or precede the majority of reactions occurring in the reaction chamber 11 , using known data analysis techniques. In certain embodiments, the skilled artisan may use procedures to synchronize the deconstruction of multiple nucleic acid molecules 13 present in a single reaction chamber 11 , as by adding a bolus of deconstruction reagents 15 with rapid mixing.
- a tag molecule may be added to the reaction chamber 11 or to the flow path 12 upstream of the detection unit 18 .
- the tag molecule binds to and tags free nucleotides 16 as they are released from the nucleic acid molecule 13 .
- This post-release tagging avoids problems that are encountered when the nucleotides 16 of the nucleic acid molecule 13 are tagged before their release into solution.
- the use of bulky fluorescent probe molecules may provide considerable steric hindrance when each nucleotide 16 incorporated into a nucleic acid molecule 13 is labeled before deconstruction, reducing the efficiency and increasing the time required for the sequencing reaction.
- nucleotides 16 In embodiments involving post-release tagging of nucleotides 16 , it is contemplated that alternative methods of detection may be used, for example fluorescence spectroscopy or luminescence spectroscopy. Many alternative methods of detection of free nucleotides 16 in solution are known and may be used. For such methods, the Raman spectroscopic detection unit 18 may be replaced with a detection unit 18 designed to detect fluorescence, luminescence or other types of signals.
- the tag molecules have unique and highly visible optical signatures that can be distinguished for each of the common nucleotides 16 .
- the tag may serve to increase the strength of the Raman emission signal or to otherwise enhance the sensitivity or specificity of the Raman detector 21 for nucleotides 16 .
- Non-limiting examples of tag molecules that could be used for embodiments involving Raman spectroscopy include TRIT (tetramethyl rhodamine isothiol), NBD (7-nitrobenz-2-oxa-1,3-diazole), Texas Red dye, phthalic acid, terephthalic acid, isophthalic acid, cresyl fast violet, cresyl blue violet, brilliant cresyl blue, para-aminobenzoic acid, erythrosine and aminoacridine.
- Other tag moieties that may be of use for particular embodiments include cyanide, thiol, chlorine, bromine, methyl, phosphorus and sulfur.
- carbon nanotubes may be of use as Raman tags.
- Raman tags in Raman spectroscopy is known in the art (e.g., U.S. Pat. Nos. 5,306,403 and 6,174,677). The skilled artisan will realize that Raman tags should generate distinguishable Raman spectra when bound to different nucleotides 16 , or different labels should be designed to bind only one type of nucleotide 16 .
- the nucleic acid molecule 13 is fixed in place, as by attachment to an immobilization surface 14 , and immersed in a microfluidic flow down a flow path 12 that transports the released nucleotides 16 away from the nucleic acid molecule 13 and past a detection unit 18 .
- the microfluidic flow may result from a bulk flow of solvent past the nucleic acid molecule 13 and down a flow path 12 , for example, a microcapillary tube or an etched channel in a silicon, glass or other chip.
- the bulk medium moves only slowly or not at all, but charged species within the solution (such as negatively charged nucleotides 16 ) move down a flow path 12 comprising a channel or tube in response to an externally applied electrical field.
- the nucleic acid molecule 13 may be moved by moving the immobilization surface 14 to which it is attached away from the released nucleotides 16 .
- the released nucleotides 16 may be scanned and identified by a moving detection unit 18 that follows the nucleic acid molecule 13 .
- the detection unit 18 must be capable of distinguishing between the common nucleotides 16 released from the nucleic acid molecule 13 . At a minimum, the detection unit 18 must be able to distinguish between nucleotides 16 containing adenosine (A), guanosine (G), cytosine (C) and thymidine (T) for sequencing DNA molecules 13 . If RNA 13 is being sequenced, the detection unit 18 must be able to distinguish between nucleotides 16 containing A, G, C and uridine (U).
- the detection unit 18 be capable of quantifying the amounts of each nucleotide 16 in solution, since the nucleotides 16 move past the detection unit 18 one at a time.
- the detection unit 107 is sensitive enough to quantify the number of free nucleotides 104 present in solution. Thus, separation of released nucleotides 104 from the nucleic acid molecule 102 is not required.
- the apparatus 100 comprises a reaction chamber 101 containing a nucleic acid molecule 102 attached at one end to an immobilization surface 103 . Free nucleotides 104 are sequentially removed from the unattached end 105 of the nucleic acid molecule 102 by the action of a deconstruction reagent 106 , such as an exonuclease.
- the free nucleotides 104 are not spatially segregated from the nucleic acid molecule 102 .
- the detection unit 107 comprising an excitation source 108 emitting an excitation beam 110 and a detector 109 , analyzes the reaction chamber 101 containing both nucleic acid molecule 102 and free nucleotides 104 . Because the detector 109 can quantify the amount of each nucleotide 104 in solution, the nucleic acid 102 sequence can be determined by the temporal sequence of release of nucleotides 104 into solution. Because a greater volume is being scanned, it may be necessary to employ a more intense excitation beam 110 covering a broader area.
- the nucleotide 104 is identified by the increase in signal for that nucleotide 104 in solution.
- the Raman detector 109 can separately quantify the number of molecules of each nucleotide 104 —adenosine monophosphate (AMP), guanosine monophosphate (GMP), cytosine monophosphate (CMP) and uridine monophosphate (UMP) or thymidine monophosphate (TMP) in solution and separate those signals from the baseline Raman signals produced by the nucleic acid molecule 102 .
- AMP adenosine monophosphate
- GMP guanosine monophosphate
- CMP cytosine monophosphate
- UMP uridine monophosphate
- TMP thymidine monophosphate
- nucleoside monophosphates 16 , 104 will generally be the form released by exonuclease 15 , 106 activity, the embodiments are not limited to detection of any particular form of free nucleotide or nucleoside 16 , 104 but encompass any monomer 16 , 104 that may be released from a nucleic acid 13 , 102 by the activity of a deconstruction reagent 15 , 106 .
- some combination of the methods and apparatus 10 , 100 shown in FIG. 1 and FIG. 2 may be used.
- the method of FIG. 1 can use a less sensitive detector 21 , but requires more complicated molecular transport procedures.
- the method of FIG. 2 has simplified molecular transport but requires a more sensitive detector 109 .
- a variety of intermediate approaches may be used. For example, a microfluidics flow down a flow path 12 may be used to remove nucleotides 16 that have already been detected from the area illuminated by the excitation beam 20 , while the quantification capability of the Raman detector 21 allows detection to occur in the absence of a specific temporal or spatial separation between the released nucleotides 16 .
- data from a detector 21 , 109 may flow to an information processing system that maintains a database associating specific Raman signatures with specific nucleotides 16 , 104 .
- the information processing system records the signatures detected by the detector 21 , 109 , correlates those signatures with the signatures of known nucleotides 16 , 104 in the database, and maintains a record of nucleotide 16 , 104 appearance that indicates the sequence of the nucleic acid molecule 13 , 102 .
- the information processing system may also perform standard procedures such as subtraction of background signals and “base-calling” determination when overlapping temporal or spatial signals are detected from more than one nucleotide 16 , 104 .
- the nucleic acid molecule 13 , 102 may be attached to a surface 14 , 103 such as functionalized glass, silicon, PDMS (polydimethlyl siloxane), silver or other metal coated surfaces, quartz, plastic, PTFE (polytetrafluoroethylene), PVP (polyvinyl pyrrolidone), polystyrene, polypropylene, polyacrylamide, latex, nylon, nitrocellulose, a glass bead, a magnetic bead, or any other material known in the art that is capable of having functional groups such as amino, carboxyl, thiol, hydroxyl or Diels-Alder reactants incorporated on its surface 14 , 103 .
- a surface 14 , 103 such as functionalized glass, silicon, PDMS (polydimethlyl siloxane), silver or other metal coated surfaces, quartz, plastic, PTFE (polytetrafluoroethylene), PVP (polyvinyl pyrrolidone),
- functional groups may be covalently attached to cross-linking agents so that binding interactions between nucleic acid molecule 13 , 102 and deconstruction reagent 15 , 106 may occur without steric hindrance.
- Typical cross-linking groups include ethylene glycol oligomers and diamines. Attachment may be by either covalent or non-covalent binding.
- Various methods of attaching nucleic acid molecules 13 , 102 to surfaces 14 , 103 are known in the art and may be employed.
- Nucleic acid 13 , 102 encompasses DNA, RNA, single-stranded, double-stranded or triple stranded and any chemical modifications thereof, although single-stranded nucleic acids 13 , 102 are preferred. Virtually any modification of the nucleic acid 13 , 102 is contemplated.
- a single stranded nucleic acid 13 , 102 may be denoted by the prefix “ss”, a double stranded nucleic acid 13 , 102 by the prefix “ds”, and a triple stranded nucleic acid 13 , 102 by the prefix “ts.”
- a “nucleic acid” 13 , 102 may be of almost any length, from 10, 20, 30, 40, 50, 60, 75, 100, 125, 150, 175, 200, 225, 250, 275, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000, 6000, 7000, 8000, 9000, 10,000, 15,000, 20,000, 30,000, 40,000, 50,000, 75,000, 100,000, 150,000, 200,000, 500,000, 1,000,000, 1,500,000, 2,000,000, 5,000,000 or even more bases in length, up to a full-length chromosomal DNA molecule 13 , 102 .
- a “nucleoside” 16 , 104 is a molecule comprising a base (A, C, G, T or U) covalently attached to a pentose sugar such as deoxyribose, ribose or derivatives or analogs of pentose sugars.
- nucleotide 16 , 104 refers to a nucleoside further comprising at least one phosphate group covalently attached to the pentose sugar.
- the nucleotides 16 , 104 are ribonucleoside monophosphates 16 , 104 or deoxyribonucleoside monophosphates 16 , 104 although in certain embodiments it is anticipated that nucleoside diphosphates or triphosphates 16 , 104 could be produced.
- nucleosides 16 , 104 may be released from the nucleic acid molecule 13 , 102 and detected as discussed below.
- nucleotides 16 , 104 may be substituted with another pentose sugar or a pentose sugar analog.
- phosphate groups may be substituted by various analogs.
- Nucleic acid molecules 13 , 102 to be sequenced may be prepared by any technique known in the art.
- the nucleic acids 13 , 102 are naturally occurring DNA or RNA molecules.
- Virtually any naturally occurring nucleic acid 13 , 102 may be prepared and sequenced by the disclosed methods including, without limit, chromosomal, mitochondrial and chloroplast DNA and ribosomal, transfer, heterogeneous nuclear and messenger RNA.
- nucleic acids 13 , 102 Methods for preparing and isolating various forms of cellular nucleic acids 13 , 102 are known. (See, e.g., Guide to Molecular Cloning Techniques, eds. Berger and Kimmel, Academic Press, New York, N.Y., 1987; Molecular Cloning: A Laboratory Manual, 2nd Ed., eds. Sambrook, Fritsch and Maniatis, Cold Spring Harbor Press, Cold Spring Harbor, N.Y., 1989). Generally, cells, tissues or other source material containing nucleic acids 13 , 102 to be sequenced are first homogenized, for example by freezing in liquid nitrogen followed by grinding in a mortar and pestle.
- Certain tissues may be homogenized using a Waring blender, Virtis homogenizer, Dounce homogenizer or other homogenizer. Crude homogenates may be extracted with detergents, such as sodium dodecyl sulphate (SDS), Triton X-100, CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate), octylglucoside or other detergents known in the art. Alternatively or in addition, extraction may use chaotropic agents such as guanidinium isothiocyanate, or organic solvents such as phenol. In some embodiments, protease treatment, for example with proteinase K, may be used to degrade cell proteins.
- detergents such as sodium dodecyl sulphate (SDS), Triton X-100, CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate),
- Particulate contaminants may be removed by centrifugation or ultracentrifugation (for example, 10 to 30 min at about 5,000 to 10,000 ⁇ g, or 30 to 60 min at about 50,000 to 100,000 ⁇ g). Dialysis against aqueous buffer of low ionic strength may be of use to remove salts or other soluble contaminants.
- Nucleic acids 13 , 102 may be precipitated by addition of ethanol at ⁇ 20° C., or by addition of sodium acetate (pH 6.5, about 0.3 M) and 0.8 volumes of 2-propanol. Precipitated nucleic acids 13 , 102 may be collected by centrifugation or, for chromosomal DNA 13 , 102 , by spooling the precipitated DNA 13 , 102 on a glass pipet or other probe.
- mitochondrial DNA 13 , 102 is often prepared by cesium chloride density gradient centrifugation, using step gradients, while mRNA 13 , 102 is often prepared using preparative columns from commercial sources, such as Promega (Madison, Wis.) or Clontech (Palo Alto, Calif.). Such variations are known in the art.
- nucleic acid 13 , 102 may be prepared using various nuclease inhibitors.
- RNase contamination in bulk solutions may be eliminated by treatment with diethyl pyrocarbonate (DEPC).
- DEPC diethyl pyrocarbonate
- Commercially available nuclease inhibitors may be obtained from standard sources such as Promega (Madison, Wis.) or BRL (Gaithersburg, Md.).
- Purified nucleic acid 13 , 102 may be dissolved in aqueous buffer, such as TE (Tris-EDTA) (ethylene diamine tetraacetic acid) and stored at ⁇ 20° C. or in liquid nitrogen prior to use.
- TE Tris-EDTA
- a ssDNA 13 , 102 may be prepared from double stranded DNA (dsDNA) by standard methods. Most simply, dsDNA may be heated above its annealing temperature, at which point it spontaneously separates into ssDNA 13 , 102 . Representative conditions might involve heating at 92 to 95° C. for 5 min or longer. Formulas for determining conditions to separate dsDNA, based for example on GC content and the length of the molecule, are known in the art.
- single-stranded DNA 13 , 102 may be prepared from double-stranded DNA by standard amplification techniques known in the art, using a primer that only binds to one strand of double-stranded DNA.
- Other methods of preparing single-stranded DNA 13 , 102 are known in the art, for example by inserting the double-stranded nucleic acid to be sequenced into the replicative form of a phage like M13, and allowing the phage to produce single-stranded copies of the nucleic acid 13 , 102 .
- nucleic acids 13 , 102 Although certain embodiments concern preparation of naturally occurring nucleic acids 13 , 102 , virtually any type of nucleic acid 13 , 102 that can serve as a substrate for an exonuclease or other deconstruction reagent 15 , 106 could potentially be sequenced.
- PCRm polymerase chain reaction
- Nucleic acids 13 , 102 to be sequenced may alternatively be cloned in standard vectors, such as plasmids, cosmids, BACs (bacterial artificial chromosomes) or YACs (yeast artificial chromosomes).
- standard vectors such as plasmids, cosmids, BACs (bacterial artificial chromosomes) or YACs (yeast artificial chromosomes).
- Nucleic acid inserts 13 , 102 may be isolated from vector DNA, for example, by excision with appropriate restriction endonucleases, followed by agarose gel electrophoresis and ethidium bromide staining.
- Selected size-fractionated nucleic acids 13 , 102 may be removed from gels, for example by the use of low melting point agarose or by electroelution from gel slices. Methods for isolation of insert nucleic acids 13 , 102 are known in the art.
- the nucleic acid molecule 13 , 102 to be sequenced is a single molecule of ssDNA or ssFWA.
- a variety of methods for selection and manipulation of single ssDNA or ssRNA molecules 13 , 102 may be used, for example, hydrodynamic focusing, micro-manipulator coupling, optical trapping, or a combination of these and similar methods. (See, e.g., Goodwin et al., 1996, Acc. Chem. Res. 29:607-619; U.S. Pat. Nos. 4,962,037; 5,405,747; 5,776,674; 6,136,543; 6,225,068.)
- microfluidics or nanofluidics may be used to sort and isolate nucleic acid molecules 13 , 102 .
- Hydrodynamics may be used to manipulate the movement of nucleic acids 13 , 102 into a microchannel, microcapillary, or a micropore.
- hydrodynamic forces may be used to move nucleic acid molecules 13 , 102 across a comb structure to separate single nucleic acid molecules 13 , 102 .
- hydrodynamic focusing may be used to position the molecules 13 , 102 within the reaction chamber 11 , 101 .
- a thermal or electric potential, pressure or vacuum can also be used to provide a motive force for manipulation of nucleic acids 13 , 102 .
- manipulation of nucleic acids 13 , 102 for sequencing may involve the use of a channel block design incorporating microfabricated channels and an integrated gel material, as disclosed in U.S. Pat. Nos. 5,867,266 and 6,2 14,246.
- a sample containing the nucleic acid molecule 13 , 102 may be diluted prior to coupling to an immobilization surface 14 , 103 .
- the immobilization surface 14 , 103 may be in the form of magnetic or non-magnetic beads or other discrete structural units. At an appropriate dilution, each bead 14 , 103 will have a statistical probability of binding zero or one nucleic acid molecule 13 , 102 . Beads 14 , 103 with one attached nucleic acid molecule 13 , 102 may be identified using, for example, fluorescent dyes and flow cytometer sorting or magnetic sorting.
- nucleic acids 13 , 102 may be sequenced.
- a coated fiber tip 14 , 103 may be used to generate single molecule nucleic acids 13 , 102 for sequencing (e.g., U.S. Pat. No. 6,225,068).
- the immobilization surfaces 14 , 103 may be prepared to contain a single molecule of avidin or other cross-linking agent. Such a surface 14 , 103 could attach a single biotinylated nucleic acid molecule 13 , 102 to be sequenced.
- This embodiment is not limited to the avidin-biotin binding system, but may be adapted to any coupling system known in the art.
- an optical trap may be used for manipulation of single molecule nucleic acid molecules 13 , 102 for sequencing.
- Exemplary optical trapping systems are commercially available from Cell Robotics, Inc. (Albuquerque, N. Mex.), S+L GmbH (Heidelberg, Germany) and P.A.L.M. Gmbh (Wolfratshausen, Germany).
- the nucleic acid molecules 13 , 102 to be sequenced may be attached to a solid surface 14 , 103 (or immobilized). Immobilization of nucleic acid molecules 13 , 102 may be achieved by a variety of methods involving either non-covalent or covalent attachment between the nucleic acid molecule 13 , 102 and the surface 14 , 103 . In an exemplary embodiment, immobilization may be achieved by coating a surface 14 , 103 with streptavidin or avidin and the subsequent attachment of a biotinylated nucleic acid 13 , 102 (Holmstrom et al., Anal. Biochem. 209:278-283, 1993).
- Immobilization may also occur by coating a silicon, glass or other surface 14 , 103 with poly-L-Lys (lysine) or poly L-Lys, Phe (phenylalanine), followed by covalent attachment of either amino- or sulfhydryl-modified nucleic acids 13 , 102 using bifunctional crosslinking reagents (Running et al., BioTechnigues 8:276-277, 1990; Newton et al., Nucleic Acids Res. 21:1155-62, 1993). Amine residues may be introduced onto a surface 14 , 103 through the use of aminosilane for cross-linking.
- Immobilization may take place by direct covalent attachment of 5 ′-phosphorylated nucleic acids 13 , 102 to chemically modified surfaces 14 , 103 (Rasmussen et al., Anal. Biochem. 198:138-142, 1991).
- the covalent bond between the nucleic acid 13 , 102 and the surface 14 , 103 is formed by condensation with a water-soluble carbodiimide. This method facilitates a predominantly 5 ′-attachment of the nucleic acids 13 , 102 via their 5 ′-phosphates.
- DNA 13 , 102 is commonly bound to glass by first silanizing the glass surface 14 , 103 , then activating with carbodiimide or glutaraldehyde. Alternative procedures may use reagents such as 3-glycidoxypropyltrimethoxysilane (GOP) or aminopropyltrimethoxysilane (APTS) with DNA 13 , 102 linked via amino linkers incorporated either at the 3′ or 5′ end of the molecule. DNA 13 , 102 may be bound directly to membrane surfaces 14 , 103 using ultraviolet radiation. Other non-limiting examples of immobilization techniques for nucleic acids 13 , 102 are disclosed in U.S. Pat. Nos. 5,610,287, 5,776,674 and 6,225,068.
- the type of surface 14 , 103 to be used for immobilization of the nucleic acid 13 , 102 is not limiting.
- the immobilization surface 14 , 103 may be magnetic beads, non-magnetic beads, a planar surface, a pointed surface, or any other conformation of solid surface 14 , 103 comprising almost any material, so long as the material is sufficiently durable and inert to allow the nucleic acid 13 , 102 sequencing reaction to occur.
- Non-limiting examples of surfaces 14 , 103 that may be used include glass, silica, silicate, PDMS, silver or other metal coated surfaces, nitrocellulose, nylon, activated quartz, activated glass, polyvinylidene difluoride (PVDF), polystyrene, polyacrylamide, other polymers such as poly(vinyl chloride), poly(methyl methacrylate) or poly(dimethyl siloxane), and photopolymers which contain photoreactive species such as nitrenes, carbenes and ketyl radicals capable of forming covalent links with nucleic acid molecules 13 , 102 (See U.S. Pat. Nos. 5,405,766 and 5,986,076).
- Bifunctional cross-linking reagents may be of use in various embodiments, such as attaching a nucleic acid molecule 13 , 102 to a surface 14 , 103 .
- the bifunctional cross-linking reagents can be divided according to the specificity of their functional groups, e.g., amino, guanidino, indole, or carboxyl specific groups. Of these, reagents directed to free amino groups are popular because of their commercial availability, ease of synthesis and the mild reaction conditions under which they can be applied. Exemplary methods for cross-linking molecules are disclosed in U.S. Pat. Nos. 5,603,872 and 5,401,511.
- Cross-linking reagents include glutaraldehyde (GAD), bifunctional oxirane (OXR), ethylene glycol diglycidyl ether (EGDE), and carbodiimides, such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC).
- GAD glutaraldehyde
- OXR bifunctional oxirane
- EGDE ethylene glycol diglycidyl ether
- EDC 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
- the sequencing reaction involves binding of a deconstruction reagent 15 , 106 to the free end 17 , 105 of the nucleic acid molecule 13 , 102 and removal of nucleotides 16 , 104 one at a time.
- the reaction may be catalyzed by an enzyme, such as an exonuclease 15 , 106 .
- the embodiments are not limited by the type of exonuclease 15 , 106 that may be used.
- Non-limiting examples of exonucleases 15 , 106 of potential use include E. coli exonuclease I, III, V or VII, Bal31 exonuclease, mung bean exonuclease, S1 nuclease, E.
- DNA polymerase I holoenzyme or Klenow fragment, RecJ, exonuclease T, T4 or T7 DNA polymerase, Taq polymerase, exonuclease T7 gene 6, snake venom phosphodiesterase, spleen phosphodiesterase, Thermococcus litoralis DNA polymerase, Pyrococcus sp. GB-D DNA polymerase, lambda exonuclease, S. aureus micrococcal nuclease, DNase I, ribonuclease A, T1 micrococcal nuclease, or other exonucleases known in the art.
- Exonucleases 15 , 106 are available from commercial sources such as New England Biolabs (Beverly, Mass.), Amersham Pharmacia Biotech (Piscataway, N.J.), Promega (Madison, Wis.), Sigma Chemicals (St. Louis, Mo.) or Boehringer Mannheim (Indianapolis, Ind.).
- enzymes with exonuclease 15 , 106 activity have various properties, for example, they can remove nucleotides 16 , 104 from the 5′ end, the 3′ end, or either end of the nucleic acid molecule 13 , 102 . They can show specificity for RNA, DNA or both RNA and DNA 13 , 102 . Their activity may depend on the use of either single or double-stranded nucleic acids 13 , 102 . They may be differentially affected by various characteristics of the reaction medium, such as salt, temperature, pH, or divalent cations. These and other properties of the various exonucleases and polymerases 15 , 106 are known in the art.
- the rate of exonuclease 15 , 106 activity may be manipulated to coincide with the optimal rate of analysis of nucleotides 16 , 104 by the detection unit 18 , 107 .
- Various methods are known for adjusting the rate of exonuclease 15 , 106 activity, including adjusting the temperature, pressure, pH, salt concentration or divalent cation concentration in the reaction chamber 11 , 101 . Methods of optimization of exonuclease 15 , 106 activity are known in the art.
- Certain embodiments may involve incorporating a label into the nucleotides 16 , 104 , to facilitate their measurement by the detection unit 18 , 107 .
- labels may be used, such as Raman tags, fluorophores, chromophores, radioisotopes, enzymatic tags, antibodies, chemiluminescent, electroluminescent, affinity labels, etc.
- Raman tags fluorophores, chromophores, radioisotopes, enzymatic tags, antibodies, chemiluminescent, electroluminescent, affinity labels, etc.
- the label moiety to be used may be a fluorophore, such as Alexa 350, Alexa 430, AMCA (7-amino-4-methylcoumarin-3-acetic acid), BODIPY (57-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid) 630/650, BODIPY 650/665, BODIPY-FL (fluorescein), BODIPY-R6G (6-carboxyrhodamine), BODIPY-TMR (tetramethylrhodamine), BODIPY-TRX (Texas Red-X), Cascade Blue, Cy2 (cyanine), Cy3, Cy5, 6-FAM (5-carboxyfluorescein), Fluorescein, 6-JOE (2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein), Oregon Green 488,
- fluorophore such as Alexa 350, Alexa 430, AMCA (7-a
- the reaction chamber 11 , 101 is designed to hold the immobilization surface 14 , 103 , nucleic acid molecule 13 , 102 , deconstruction reagent 15 , 106 and nucleotides 16 , 104 in an aqueous environment.
- the reaction chamber 11 , 101 is designed to be temperature controlled, for example by incorporation of Pelletier elements or other methods known in the art. Methods of controlling temperature for low volume liquids are known in the art. (See, e.g., U.S. Pat. Nos. 5,038,853, 5,919,622, 6,054,263 and 6,180,372.)
- the reaction chamber 11 , 101 and any associated fluid channels for example, the flow path 12 or channels to provide connections to a waste port, to a nucleic acid 13 , 102 loading port, or to a source of deconstruction reagent 15 , 106 are manufactured in a batch fabrication process, as known in the fields of computer chip manufacture or microcapillary chip manufacture.
- the reaction chamber 11 , 101 and other components of the apparatus 10 , 100 may be manufactured as a single integrated chip. Such a chip may be manufactured by methods known in the art, such as by photolithography and etching.
- the manufacturing method is not limiting and other methods known in the art may be used, such as laser ablation, injection molding, casting, or imprinting techniques. Methods for manufacture of nanoelectromechanical systems may be used for certain embodiments. (See, e.g., Craighead, Science 290: 1532-36, 2000.) Microfabricated chips are commercially available from sources such as Caliper Technologies Inc. (Mountain View, Calif.) and ACLARA BioSciences Inc. (Mountain View, Calif.).
- Borofloat glass wafers may be pre-etched for a short period in concentrated HF (hydrofluoric acid) and cleaned before deposition of an amorphous silicon sacrificial layer in a plasma-enhanced chemical vapor deposition (PECVD) system (PEII-A, Technics West, San Jose, Calif.). Wafers may be primed with hexamethyldisilazane ( ⁇ &IDS), spin-coated with photoresist (Shipley 1818, Marlborough, Mass.) and soft-baked.
- a contact mask aligner Quintel Corp.
- CMOS complementary metal-oxide-semiconductor
- CF4 carbon tetrafluoride
- Wafers may be chemically etched with concentrated HF to produce the reaction chamber 11 , 101 , flow path 12 and any channels. The remaining photoresist may be stripped and the amorphous silicon removed.
- Access holes may be drilled into the etched wafers with a diamond drill bit (Crystalite, Westerville, Ohio).
- a finished chip may be prepared by thermally bonding an etched and drilled plate to a flat wafer of the same size in a programmable vacuum furnace (Centurion VPM, J. M. Ney, Yucaipa, Calif.). In certain embodiments, the chip may be prepared by bonding two etched plates to each other.
- Alternative exemplary methods for fabrication of a chip incorporating a reaction chamber 11 , 101 and flow path 12 are disclosed in U.S. Pat. Nos. 5,867,266 and 6,214,246.
- the material comprising the reaction chamber 11 , 101 and/or flow path 12 may be selected to be transparent to electromagnetic radiation at the excitation and emission frequencies used for the detection unit 18 , 107 .
- Glass, silicon, and any other materials that are generally transparent in the frequency ranges used for Raman spectroscopy, fluorescence spectroscopy, luminescence spectroscopy, or other forms of spectroscopy may be used.
- the surfaces of the reaction chamber 11 , 101 and/or flow path 12 that are opposite the detection unit 18 , 107 may be coated with silver, gold, platinum, copper, aluminum or other materials that are relatively opaque to the detection unit 18 , 107 .
- the opaque material is available to enhance the Raman or other signal, for example by surface enhanced Raman spectroscopy, while not interfering with the function of the detection unit 18 , 107 .
- the reaction chamber 11 , 101 and/or flow path 12 may contain a mesh comprising silver, gold, platinum, copper or aluminum.
- the nucleotides 16 will generally be detected while they are in the flow path 12 .
- the nucleotides 104 will be detected in the reaction chamber 101 .
- the reaction chamber 11 , 101 may have an internal volume of about 1 picoliter, about 2 picoliters, about 5 picoliters, about 10 picoliters, about 20 picoliters, about 50 picoliters, about 100 picoliters, about 250 picoliters, about 500 picoliters, about 1 nanoliter, about 2 nanoliters, 5 nanoliters, about 10 nanoliters, about 20 nanoliters, about 50 nanoliters, about 100 nanoliters, about 250 nanoliters, about 500 nanoliters, about 1 microliter, about 2 microliters, about 5 microliters, about 10 microliters, about 20 microliters, about 50 microliters, about 100 microliters, about 250 microliters, about 500 microliters, or about 1 milliliter.
- the free nucleotides 16 are moved down a flow path 12 past the detection unit 18 .
- a non-limiting example of techniques for transport of free nucleotides 16 includes microfluidic techniques.
- the flow path 12 can comprise a microcapillary (available, e.g., from ACLARA BioSciences Inc., Mountain View, Calif.) or a liquid integrated circuit (e.g., Caliper Technologies Inc., Mountain View, Calif.). Such microfluidic platforms require only nanoliter volumes of sample.
- the free nucleotides 16 to be detected move down the flow path 12 by bulk flow of solvent.
- microcapillary electrophoresis may be used to transport free nucleotides 16 down the flow path 12 and past the detection unit 18 .
- Microcapillary electrophoresis generally involves the use of a thin capillary or channel that may or may not be filled with a particular separation medium. Electrophoresis of appropriately charged molecular species, such as negatively charged nucleotides 16 , occurs in response to an imposed electrical field, negative on the reaction chamber 11 side of the apparatus and positive on the detection unit 18 side.
- electrophoresis is often used for size separation of a mixture of components that are simultaneously added to the microcapillary, it can also be used to transport similarly sized nucleotides 16 that are sequentially added to the flow path 12 . Because the purine nucleotides (A, G) 16 are larger than the pyrimidine nucleotides (C, T, U) 16 and would therefore migrate more slowly, the length of the flow path 12 and corresponding transit time past the detector unit 18 should be kept to a minimum to prevent differential migration from mixing up the order of nucleotides 16 released from the nucleic acid 13 .
- the separation medium filling the microcapillary may be selected so that the migration rates of purine and pyrimidine nucleotides 16 down the flow path 12 are similar or identical.
- Methods of microcapillary electrophoresis have been disclosed, for example, by Woolley and Mathies ( Proc. Natl. Acad. Sci. USA 91:11348-352, 1994).
- microfabrication of microfluidic devices has been discussed in, e.g., Jacobsen et al. (Anal. Biochem, 209:278-283, 1994); Effenhauser et al. (Anal. Chem. 662949-2953, 1994); Harrison et ol. (Science 261 :895-897, 1993) and U.S. Pat. No. 5,904,824.
- these methods comprise photolithographic etching of micron scale channels on silica, silicon or other crystalline substrates or chips, and can be readily adapted for use.
- the microcapillary may be fabricated from the same polymeric materials described for the fabrication of the reaction chamber 11 , 101 , using injection molding or other techniques known in the art.
- the detection unit 18 , 107 is designed to detect and quantify nucleotides 16 , 104 by Raman spectroscopy.
- Raman spectroscopy Various methods for detection of nucleotides 16 , 104 by Raman spectroscopy are known in the art. (See, e.g., U.S. Pat. Nos. 5,306,403; 6,002,471; 6,174,677). Variations on surface enhanced Raman spectroscopy (SERS) or surface enhanced resonance Raman spectroscopy (SERRS) have been disclosed. In SERS and SERRS, the sensitivity of the Raman detection is enhanced by a factor of 1o6 or more for molecules adsorbed on roughened metal surfaces, such as silver, gold, platinum, copper or aluminum surfaces.
- SERS surface enhanced Raman spectroscopy
- SERRS surface enhanced resonance Raman spectroscopy
- the excitation beam 20 , 110 is generated by either a Nd:YAG laser 19 , 108 at 532 nm wavelength or a Ti:sapphire laser 19 , 108 at 365 nm wavelength. Pulsed laser beams 20 , 110 or continuous laser beams 20 , 110 may be used. The excitation beam 20 , 110 passes through confocal optics and a microscope objective, and is focused onto the flow path 12 or the reaction chamber 101 .
- the Raman emission light from the nucleotides 16 , 104 is collected by the microscope objective and the confocal optics and is coupled to a monochromator for spectral dissociation.
- the confocal optics includes a combination of dichroic filters, barrier filters, confocal pinholes, lenses, and mirrors for reducing the background signal. Standard full field optics can be used as well as confocal optics.
- the Raman emission signal is detected by a Raman detector 21 , 109 .
- the detector 21 , 109 includes an avalanche photodiode interfaced with a computer for counting and digitization of the signal.
- a mesh comprising silver, gold, platinum, copper or aluminum may be included in the flow path 12 or the reaction chamber 101 to provide an increased signal due to surface enhanced Raman or surface enhanced Raman resonance.
- detection units 18 , 107 are disclosed, for example, in U.S. Pat. No. 5,306,403, including a Spex Model 1403 double-grating spectrophotometer 21 , 109 equipped with a gallium-arsenide photomultiplier tube (RCA Model C31034 or Burle Industries Model C3 103402) operated in the single-photon counting mode.
- the excitation source 19 , 108 is a 514.5 nm line argon-ion laser 19 , 108 from SpectraPhysics, Model 166, and a 647.1 nm line of a krypton-ion laser 19 , 108 (Innova 70, Coherent).
- Alternative excitation sources 19 , 108 include a nitrogen laser 19 , 108 (Laser Science Inc.) at 337 nm and a helium-cadmium laser 19 , 108 (Liconox) at 325 nm (U.S. Pat. No. 6,174,677).
- the excitation beam 20 , 110 may be spectrally purified with a bandpass filter (Corion) and may be focused on the flow path 12 or reaction chamber 101 using a 6 ⁇ objective lens (Newport, Model L6X).
- the objective lens may be used to both excite the nucleotides 16 , 104 and to collect the Raman signal, by using a holographic beam splitter (Kaiser Optical Systems, Inc., Model HB 647-26N18) to produce a right-angle geometry for the excitation beam 20 , 110 and the emitted Raman signal.
- a holographic notch filter (Kaiser Optical Systems, Inc.) may be used to reduce Rayleigh scattered radiation.
- Alternative Raman detectors 21 , 109 include an ISA HR-320 spectrograph equipped with a red-enhanced intensified charge-coupled device (RE-ICCD) detection system (Princeton Instruments). Other types of detectors 21 , 109 may be used, such as charged injection devices, photodiode arrays or phototransistor arrays.
- Raman spectroscopy or related techniques may be used for detection of nucleotides 16 , 104 , including but not limited to normal Raman scattering, resonance Raman scattering, surface enhanced Raman scattering, surface enhanced resonance Raman scattering, coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman scattering, inverse Raman spectroscopy, stimulated gain Raman spectroscopy, hyper-Raman scattering, molecular optical laser examiner (MOLE) or Raman microprobe or Raman microscopy or confocal Raman microspectrometry, three-dimensional or scanning Raman, Raman saturation spectroscopy, time resolved resonance Raman, Raman decoupling spectroscopy or UV-Raman microscopy.
- CARS coherent anti-Stokes Raman spectroscopy
- MOLE molecular optical laser examiner
- Raman microprobe or Raman microscopy or confocal Raman microspectrometry three-dimensional or scanning Raman, Raman saturation spectroscopy, time resolved
- the nucleotides 16 , 104 are identified and quantified using fluorescence resonance energy transfer (FRET).
- FRET is a spectroscopic phenomenon used to detect proximity between a donor molecule and an acceptor molecule.
- the donor and acceptor pairs are chosen such that fluorescent emission from the donor overlaps the excitation spectrum of the acceptor.
- the excited-state energy of the donor is transferred non-radiatively to the acceptor and the donor emission is quenched. If the acceptor molecule is a fluorophore then its emission is enhanced.
- Compositions and methods for use of FRET with oligonucleotides are known in the art (e.g., U.S. Pat. No. 5,866,366).
- Molecules that are frequently used as tags for FRET include fluorescein, 5-carboxyfluorescein (FAM), 2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein (JOE), rhodamine, 6-carboxyrhodamine (R6G), N,N,N′,N′-tetramethyl-6-carboxyrhodamine (TAMRA), 6-carboxy-X-rhodamine (ROX), 4-(4′-dimethylaminophenylazo) benzoic acid (DABCYL), and 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid (EDANS).
- FAM 5-carboxyfluorescein
- JE 2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein
- rhodamine 6-carboxyrhodamine
- R6G 6-car
- FRET donor or acceptor molecules are known in the art (See U.S. Pat. No. 5,866,336, Table 1). The skilled artisan will be familiar with the selection of pairs of tag molecules for FRET (U.S. Pat. No. 5,866,336).
- the donor and acceptor molecules may be covalently or non-covalently attached to various constituents of the sequencing apparatus 10 , 100 .
- the donor or acceptor molecules may be attached to the nucleotides 16 , 104 , to the exonuclease 15 , 106 , or to the flow path 12 .
- the donor molecule may be attached to the exonuclease 15 , 106 or to the surface of the flow path 12 and the acceptor molecules attached to the nucleotides 16 , 104 .
- each type of nucleotide 16 , 104 should be attached to an acceptor molecule with a distinguishable emission spectrum, while the donor molecule should be selected to have a broad emission spectrum that overlaps with the excitation spectra for all four of the acceptor molecules.
- Multiple donor molecules may be present on the exonuclease 15 , 106 or flow path 12 , although in other embodiments only a single donor molecule may be present.
- the donor molecules Upon excitation, the donor molecules will transfer their energy to the acceptor tag molecules attached to the nucleotides 16 , 104 , resulting in an enhanced emission signal from the acceptor molecules. Because the strength of the signal enhancement decreases rapidly with distance, the greatest signal enhancement will occur for nucleotides 16 , 104 that are very close to the donor molecule(s). In the case of a donor molecule attached to the exonuclease 15 , 106 , at or near the catalytic site, the nucleotide 16 , 104 with the strongest emission signal will be located at the catalytic site of the exonuclease 15 , 106 .
- the donor molecule should be attached close to the catalytic site, but in a position where it will not interfere with the exonuclease activity of the deconstruction reagent 15 , 106 .
- donor molecules are attached to the surface of the flow path 12 in the location where the excitation beam 20 is focused, only nucleotides 16 within the focus of the excitation beam 20 should give a detectable fluorescent signal.
- the wavelength of the excitation beam 20 , 110 may be selected to maximally excite the donor molecules, while only weakly exciting the acceptor molecules. As each nucleotide 16 , 104 is removed from the nucleic acid molecule 13 , 102 , the signal from its donor tag will be detected.
- the template nucleic acid 13 , 102 to be sequenced may be held within the field of view of a fluorescence microscope by methods known in the art, for example by use of an optical trap (e.g., U.S. Pat. No. 6,136,543).
- a fluorescence microscope that may be used is an inverted phase-contrast and incident-light fluorescence microscope (IMT2-RFC, Olympus Co., Ltd.), using an oil-immersed 100 power lens (Plan.multidot.Apochromat.times.100, 1.40 NA, Olympus Co., Ltd.)
- the excitation beam 20 , 110 may be emitted by a laser 19 , 108 , as discussed above. Fluorescence emission may be collected through the objective lens, using appropriate filters, and detected using any sensitive fluorescence detector 21 , 109 , such as a CCD device, photodiodes, photomultiplier tubes, or the equivalent.
- the nucleic acid sequencing apparatus 10 , 100 may comprise an information processing system.
- An exemplary information processing system may incorporate a computer comprising a bus for communicating information and a processor for processing information.
- the processor is selected from the Pentium® family of processors, including without limitation the Pentium® II family, the Pentium® III family and the Pentium® 4 family of processors available from Intel Corp. (Santa Clara, Calif.).
- the processor may be a Celeron®, an Itanium®, or a Pentium Xeon® processor (Intel Corp., Santa Clara, Calif.).
- the processor may be based on Intel® architecture, such as Intel® IA-32 or Intel® IA-64 architecture. Alternatively, other processors may be used.
- the computer may further comprise a random access memory (RAM) or other dynamic storage device, a read only memory (ROM) or other static storage and a data storage device such as a magnetic disk or optical disc and its corresponding drive.
- RAM random access memory
- ROM read only memory
- the information processing system may also comprise other peripheral devices known in the art, such a display device (e.g., cathode ray tube or Liquid Crystal Display), an alphanumeric input device (e.g., keyboard), a cursor control device (e.g., mouse, trackball, or cursor direction keys) and a communication device (e.g., modem, network interface card, or interface device used for coupling to Ethernet, token ring, or other types of networks).
- a display device e.g., cathode ray tube or Liquid Crystal Display
- an alphanumeric input device e.g., keyboard
- a cursor control device e.g., mouse, trackball, or cursor direction keys
- a communication device
- the detection unit 18 , 107 may be operably coupled to the information processing system. Data from the detection unit 18 , 107 may be processed by the processor and data stored in the main memory. Data on emission profiles for standard nucleotides 16 , 104 may also be stored in main memory or in ROM.
- the processor may compare the emission spectra from nucleotides 16 , 104 in the reaction chamber 101 or the flow path 12 to identify the type of nucleotide 16 , 104 released from the nucleic acid molecule 13 , 102 .
- the main memory may also store the sequence of nucleotides 16 , 104 released from the nucleic acid molecule 13 , 102 .
- the processor may analyze the data from the detection unit 18 , 107 to determine the sequence of the nucleic acid 13 , 102 . It is appreciated that a differently equipped information processing system may be used for certain implementations. Therefore, the configuration of the system may vary in different embodiments.
- the data will typically be reported to a data analysis operation.
- the data obtained by the detection unit 18 , 109 will typically be analyzed using a digital computer such as that described above.
- the computer will be appropriately programmed for receipt and storage of the data from the detection unit 18 , 109 as well as for analysis and reporting of the data gathered.
- custom designed software packages may be used to analyze the data obtained from the detection unit 18 , 109 .
- data analysis may be performed, using an information processing system and publicly available software packages.
- available software for DNA sequence analysis include the PRISMm DNA Sequencing Analysis Software (Applied Biosystems, Foster City, Calif.), the SequencherTM package (Gene Codes, Ann Arbor, Mich.), and a variety of software packages available through the National Biotechnology Information Facility at website www.nbif.orgilinksll.4.1.php.
Abstract
The disclosed methods, apparatus and compositions are of use for nucleic acid sequencing. More particularly, the methods and apparatus concern sequencing single molecules of single stranded DNA or RNA by exposing the molecule to exonuclease activity, removing free nucleotides one at a time from one end of the nucleic acid, and identifying the released nucleotides by Raman spectroscopy or FRET.
Description
- The present methods, compositions and apparatus relate to the fields of molecular biology and genomics. More particularly, the methods, compositions and apparatus concern nucleic acid sequencing.
- The advent of the human genome project required that improved methods for sequencing nucleic acids, such as DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), be developed. Genetic information is stored in the form of very long molecules of DNA organized into chromosomes. The twenty-three pairs of chromosomes in the human genome contain approximately three billion bases of DNA sequence. This DNA sequence information determines multiple characteristics of each individual, such as height, eye color and ethnicity. Many common diseases, such as cancer, cystic fibrosis, sickle cell anemia and muscular dystrophy are based at least in part on variations in DNA sequence.
- Determination of the entire sequence of the human genome has provided a foundation for identifying the genetic basis of such diseases. However, a great deal of work remains to be done to identify the genetic variations associated with each disease. That would require DNA sequencing of portions of chromosomes in individuals or families exhibiting each such disease, in order to identify specific changes in DNA sequence that promote the disease. RNA, an intermediary molecule required for processing of genetic information, can also be sequenced in some cases to identify the genetic bases of various diseases.
- Existing methods for nucleic acid sequencing, based on detection of fluorescently labeled nucleic acids that have been separated by size, are limited by the length of the nucleic acid that can be sequenced. Typically, only 500 to 1,000 bases of nucleic acid sequence can be determined at one time. This is much shorter than the length of the functional unit of DNA, referred to as a gene, which can be tens or even hundreds of thousands of bases in length. Using current methods, determination of a complete gene sequence requires that many copies of the gene be produced, cut into overlapping fragments and sequenced, after which the overlapping DNA sequences may be assembled into the complete gene. This process is laborious, expensive, inefficient and time-consuming.
- The following drawings form part of the present specification and are included to further demonstrate certain aspects of the disclosed embodiments. The embodiments may be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein.
-
FIG. 1 illustrates an exemplary apparatus (not to scale) and method for DNA sequencing in which the released nucleotides are spatially separated from the nucleic acid molecule to be sequenced. -
FIG. 2 illustrates an exemplary apparatus (not to scale) and method for DNA sequencing in which the released nucleotides are not spatially separated from the nucleic acid molecule. The detector quantifies the nucleotides present in solution. - The disclosed methods, compositions and apparatus are of use for the rapid, automated sequencing of nucleic acids. In particular embodiments, the methods, compositions and apparatus are suitable for obtaining the sequences of very long nucleic acid molecules of greater than 1,000, greater than 2,000, greater than 5,000, greater than 10,000, greater than 20,000, greater than 50,000, greater than 100,000 or even more bases in length. In various embodiments, such sequence information may be obtained during the course of a single sequencing run, using one molecule of
nucleic acid nucleic acid molecule nucleic acid molecule - In certain embodiments, the
nucleic acid nucleic acids - In some embodiments, disclosed in
FIG. 1 andFIG. 2 , the methods involve sequencing of individual single-strandednucleic acid molecules immobilization surface 14, 103 in areaction chamber reaction chamber more deconstruction reagents nucleotide unattached end nucleic acid molecule such deconstruction reagents nucleotides - Certain embodiments are illustrated in
FIG. 1 .FIG. 1 shows anapparatus 10 for nucleic acid sequencing comprising areaction chamber 11 attached to aflow path 12. Thereaction chamber 11 contains anucleic acid molecule 13 attached to an immobilization surface 14 along with adeconstruction reagent 15, such as an exonuclease. Theexonuclease 15 catalyzes the sequential release ofindividual nucleotides 16 from thefree end 17 of thenucleic acid molecule 13. As theindividual nucleotides 16 are released by the deconstruction reaction and enter solution, they move down theflow path 12 past adetection unit 18. Thedetection unit 18 comprises anexcitation source 19, such as a laser, that emits anexcitatory beam 20. Theexcitatory beam 20 interacts with the releasednucleotides 16 so that electrons are excited to a higher energy state. The Raman emission spectrum that results from the return of the electrons to a lower energy state is detected by a Ramanspectroscopic detector 21, such as a spectrometer or a monochromator. - In embodiments illustrated in
FIG. 1 , the releasednucleotides 16 are spatially separated from thenucleic acid molecule 13 before detection by thedetection unit 18. Spatial separation acts to increase the signal-to-noise ratio of theRaman detector 21 by isolating theindividual nucleotides 16. -
FIG. 1 illustrates embodiments in which a singlenucleic acid molecule 13 is contained in asingle reaction chamber 11. In alternative embodiments, multiplenucleic acid molecules 13, each in aseparate reaction chamber 11, may be sequenced simultaneously. In such cases, thenucleic acid molecule 13 in eachreaction chamber 11 may be identical or may be different. In other alternative embodiments, two or morenucleic acid molecules 13 may be present in asingle reaction chamber 11. In such embodiments, thenucleic acid molecules 13 will be identical in sequence. Where more than onenucleic acid molecule 13 is present in thereaction chamber 11, the Raman emission signals will represent an average of thenucleotides 16 released simultaneously from allnucleic acid molecules 13 in thereaction chamber 11. The skilled artisan will be able to correct the signal obtained at any given time for deconstruction reactions that either lag behind or precede the majority of reactions occurring in thereaction chamber 11, using known data analysis techniques. In certain embodiments, the skilled artisan may use procedures to synchronize the deconstruction of multiplenucleic acid molecules 13 present in asingle reaction chamber 11, as by adding a bolus ofdeconstruction reagents 15 with rapid mixing. - In certain alternative embodiments, a tag molecule may be added to the
reaction chamber 11 or to theflow path 12 upstream of thedetection unit 18. The tag molecule binds to and tagsfree nucleotides 16 as they are released from thenucleic acid molecule 13. This post-release tagging avoids problems that are encountered when thenucleotides 16 of thenucleic acid molecule 13 are tagged before their release into solution. For example, the use of bulky fluorescent probe molecules may provide considerable steric hindrance when eachnucleotide 16 incorporated into anucleic acid molecule 13 is labeled before deconstruction, reducing the efficiency and increasing the time required for the sequencing reaction. - In embodiments involving post-release tagging of
nucleotides 16, it is contemplated that alternative methods of detection may be used, for example fluorescence spectroscopy or luminescence spectroscopy. Many alternative methods of detection offree nucleotides 16 in solution are known and may be used. For such methods, the Ramanspectroscopic detection unit 18 may be replaced with adetection unit 18 designed to detect fluorescence, luminescence or other types of signals. - The tag molecules have unique and highly visible optical signatures that can be distinguished for each of the
common nucleotides 16. In certain embodiments, the tag may serve to increase the strength of the Raman emission signal or to otherwise enhance the sensitivity or specificity of theRaman detector 21 fornucleotides 16. Non-limiting examples of tag molecules that could be used for embodiments involving Raman spectroscopy include TRIT (tetramethyl rhodamine isothiol), NBD (7-nitrobenz-2-oxa-1,3-diazole), Texas Red dye, phthalic acid, terephthalic acid, isophthalic acid, cresyl fast violet, cresyl blue violet, brilliant cresyl blue, para-aminobenzoic acid, erythrosine and aminoacridine. Other tag moieties that may be of use for particular embodiments include cyanide, thiol, chlorine, bromine, methyl, phosphorus and sulfur. In certain embodiments, carbon nanotubes may be of use as Raman tags. The use of tags in Raman spectroscopy is known in the art (e.g., U.S. Pat. Nos. 5,306,403 and 6,174,677). The skilled artisan will realize that Raman tags should generate distinguishable Raman spectra when bound todifferent nucleotides 16, or different labels should be designed to bind only one type ofnucleotide 16. - In certain embodiments, the
nucleic acid molecule 13 is fixed in place, as by attachment to an immobilization surface 14, and immersed in a microfluidic flow down aflow path 12 that transports the releasednucleotides 16 away from thenucleic acid molecule 13 and past adetection unit 18. In non-limiting examples, the microfluidic flow may result from a bulk flow of solvent past thenucleic acid molecule 13 and down aflow path 12, for example, a microcapillary tube or an etched channel in a silicon, glass or other chip. In alternative embodiments, the bulk medium moves only slowly or not at all, but charged species within the solution (such as negatively charged nucleotides 16) move down aflow path 12 comprising a channel or tube in response to an externally applied electrical field. - In other alternative embodiments, the
nucleic acid molecule 13 may be moved by moving the immobilization surface 14 to which it is attached away from the releasednucleotides 16. The releasednucleotides 16 may be scanned and identified by a movingdetection unit 18 that follows thenucleic acid molecule 13. - In the embodiments discussed above, the
detection unit 18 must be capable of distinguishing between thecommon nucleotides 16 released from thenucleic acid molecule 13. At a minimum, thedetection unit 18 must be able to distinguish betweennucleotides 16 containing adenosine (A), guanosine (G), cytosine (C) and thymidine (T) for sequencingDNA molecules 13. IfRNA 13 is being sequenced, thedetection unit 18 must be able to distinguish betweennucleotides 16 containing A, G, C and uridine (U). With a singlenucleic acid molecule 13 perreaction chamber 11, it is not necessary that thedetection unit 18 be capable of quantifying the amounts of eachnucleotide 16 in solution, since thenucleotides 16 move past thedetection unit 18 one at a time. - In other embodiments, illustrated in
FIG. 2 , thedetection unit 107 is sensitive enough to quantify the number offree nucleotides 104 present in solution. Thus, separation of releasednucleotides 104 from thenucleic acid molecule 102 is not required. As illustrated inFIG. 2 , theapparatus 100 comprises areaction chamber 101 containing anucleic acid molecule 102 attached at one end to animmobilization surface 103.Free nucleotides 104 are sequentially removed from theunattached end 105 of thenucleic acid molecule 102 by the action of adeconstruction reagent 106, such as an exonuclease. - As shown in
FIG. 2 , in these embodiments thefree nucleotides 104 are not spatially segregated from thenucleic acid molecule 102. Thedetection unit 107, comprising anexcitation source 108 emitting anexcitation beam 110 and adetector 109, analyzes thereaction chamber 101 containing bothnucleic acid molecule 102 andfree nucleotides 104. Because thedetector 109 can quantify the amount of eachnucleotide 104 in solution, thenucleic acid 102 sequence can be determined by the temporal sequence of release ofnucleotides 104 into solution. Because a greater volume is being scanned, it may be necessary to employ a moreintense excitation beam 110 covering a broader area. - In these embodiments, as each
successive nucleotide 104 is released from theunattached end 105 of thenucleic acid molecule 102, thenucleotide 104 is identified by the increase in signal for thatnucleotide 104 in solution. TheRaman detector 109 can separately quantify the number of molecules of eachnucleotide 104—adenosine monophosphate (AMP), guanosine monophosphate (GMP), cytosine monophosphate (CMP) and uridine monophosphate (UMP) or thymidine monophosphate (TMP) in solution and separate those signals from the baseline Raman signals produced by thenucleic acid molecule 102. - The skilled artisan will realize that analysis of
DNA deoxyribonucleotides 16, 104 (including thymidine), while analysis ofRNA ribonucleotides 16, 104 (including uridine). Althoughnucleoside monophosphates exonuclease nucleoside monomer nucleic acid deconstruction reagent - In further embodiments, some combination of the methods and
apparatus FIG. 1 andFIG. 2 may be used. The method ofFIG. 1 can use a lesssensitive detector 21, but requires more complicated molecular transport procedures. The method ofFIG. 2 has simplified molecular transport but requires a moresensitive detector 109. A variety of intermediate approaches may be used. For example, a microfluidics flow down aflow path 12 may be used to removenucleotides 16 that have already been detected from the area illuminated by theexcitation beam 20, while the quantification capability of theRaman detector 21 allows detection to occur in the absence of a specific temporal or spatial separation between the releasednucleotides 16. - In certain embodiments, data from a
detector specific nucleotides detector nucleotides nucleotide nucleic acid molecule nucleotide - In certain embodiments, the
nucleic acid molecule surface 14, 103 such as functionalized glass, silicon, PDMS (polydimethlyl siloxane), silver or other metal coated surfaces, quartz, plastic, PTFE (polytetrafluoroethylene), PVP (polyvinyl pyrrolidone), polystyrene, polypropylene, polyacrylamide, latex, nylon, nitrocellulose, a glass bead, a magnetic bead, or any other material known in the art that is capable of having functional groups such as amino, carboxyl, thiol, hydroxyl or Diels-Alder reactants incorporated on itssurface 14, 103. - In some embodiments, functional groups may be covalently attached to cross-linking agents so that binding interactions between
nucleic acid molecule deconstruction reagent nucleic acid molecules surfaces 14, 103 are known in the art and may be employed. - Definitions
- As used herein, “a” or “an” may mean one or more than one of an item.
- “Nucleic acid” 13, 102 encompasses DNA, RNA, single-stranded, double-stranded or triple stranded and any chemical modifications thereof, although single-stranded
nucleic acids nucleic acid nucleic acid nucleic acid nucleic acid - A “nucleic acid” 13, 102 may be of almost any length, from 10, 20, 30, 40, 50, 60, 75, 100, 125, 150, 175, 200, 225, 250, 275, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000, 6000, 7000, 8000, 9000, 10,000, 15,000, 20,000, 30,000, 40,000, 50,000, 75,000, 100,000, 150,000, 200,000, 500,000, 1,000,000, 1,500,000, 2,000,000, 5,000,000 or even more bases in length, up to a full-length
chromosomal DNA molecule - A “nucleoside” 16, 104 is a molecule comprising a base (A, C, G, T or U) covalently attached to a pentose sugar such as deoxyribose, ribose or derivatives or analogs of pentose sugars.
- A “nucleotide” 16, 104 refers to a nucleoside further comprising at least one phosphate group covalently attached to the pentose sugar. In some embodiments, the
nucleotides ribonucleoside monophosphates deoxyribonucleoside monophosphates triphosphates nucleosides nucleic acid molecule nucleotides nucleic acid deconstruction reagent - Nucleic Acids
-
Nucleic acid molecules nucleic acids nucleic acid - Methods for preparing and isolating various forms of cellular
nucleic acids nucleic acids Nucleic acids nucleic acids chromosomal DNA DNA - The skilled artisan will realize that the procedures listed above are exemplary only and that many variations may be used, depending on the particular type of
nucleic acid mitochondrial DNA mRNA - The skilled artisan will realize that depending on the type of
nucleic acid nucleic acid - In cases where single stranded DNA (ssDNA) 13, 102 is to be sequenced, a
ssDNA ssDNA DNA DNA nucleic acid - Although certain embodiments concern preparation of naturally occurring
nucleic acids nucleic acid other deconstruction reagent nucleic acids Nucleic acids nucleic acids nucleic acids - Isolation of Single Nucleic Acid Molecules
- In certain embodiments, the
nucleic acid molecule ssRNA molecules - In certain embodiments, microfluidics or nanofluidics may be used to sort and isolate
nucleic acid molecules nucleic acids nucleic acid molecules nucleic acid molecules nucleic acid molecules molecules reaction chamber nucleic acids nucleic acids - In another embodiment, a sample containing the
nucleic acid molecule immobilization surface 14, 103. In exemplary embodiments, theimmobilization surface 14, 103 may be in the form of magnetic or non-magnetic beads or other discrete structural units. At an appropriate dilution, eachbead 14, 103 will have a statistical probability of binding zero or onenucleic acid molecule Beads 14, 103 with one attachednucleic acid molecule beads 14, 103 and thenucleic acids beads 14, 103 containing a single boundnucleic acid molecule nucleic acids other immobilization surface 14, 103 may be sequenced. - In alternative embodiments, a
coated fiber tip 14, 103 may be used to generate single moleculenucleic acids surface 14, 103 could attach a single biotinylatednucleic acid molecule - In other alternative embodiments, an optical trap may be used for manipulation of single molecule
nucleic acid molecules - Methods of Immobilization
- In various embodiments, the
nucleic acid molecules nucleic acid molecules nucleic acid molecule surface 14, 103. In an exemplary embodiment, immobilization may be achieved by coating asurface 14, 103 with streptavidin or avidin and the subsequent attachment of a biotinylatednucleic acid 13, 102 (Holmstrom et al., Anal. Biochem. 209:278-283, 1993). Immobilization may also occur by coating a silicon, glass orother surface 14, 103 with poly-L-Lys (lysine) or poly L-Lys, Phe (phenylalanine), followed by covalent attachment of either amino- or sulfhydryl-modifiednucleic acids surface 14, 103 through the use of aminosilane for cross-linking. - Immobilization may take place by direct covalent attachment of 5′-phosphorylated
nucleic acids nucleic acid surface 14, 103 is formed by condensation with a water-soluble carbodiimide. This method facilitates a predominantly 5′-attachment of thenucleic acids -
DNA glass surface 14, 103, then activating with carbodiimide or glutaraldehyde. Alternative procedures may use reagents such as 3-glycidoxypropyltrimethoxysilane (GOP) or aminopropyltrimethoxysilane (APTS) withDNA DNA nucleic acids - The type of
surface 14, 103 to be used for immobilization of thenucleic acid immobilization surface 14, 103 may be magnetic beads, non-magnetic beads, a planar surface, a pointed surface, or any other conformation ofsolid surface 14, 103 comprising almost any material, so long as the material is sufficiently durable and inert to allow thenucleic acid surfaces 14, 103 that may be used include glass, silica, silicate, PDMS, silver or other metal coated surfaces, nitrocellulose, nylon, activated quartz, activated glass, polyvinylidene difluoride (PVDF), polystyrene, polyacrylamide, other polymers such as poly(vinyl chloride), poly(methyl methacrylate) or poly(dimethyl siloxane), and photopolymers which contain photoreactive species such as nitrenes, carbenes and ketyl radicals capable of forming covalent links withnucleic acid molecules 13, 102 (See U.S. Pat. Nos. 5,405,766 and 5,986,076). - Bifunctional cross-linking reagents may be of use in various embodiments, such as attaching a
nucleic acid molecule surface 14, 103. The bifunctional cross-linking reagents can be divided according to the specificity of their functional groups, e.g., amino, guanidino, indole, or carboxyl specific groups. Of these, reagents directed to free amino groups are popular because of their commercial availability, ease of synthesis and the mild reaction conditions under which they can be applied. Exemplary methods for cross-linking molecules are disclosed in U.S. Pat. Nos. 5,603,872 and 5,401,511. Cross-linking reagents include glutaraldehyde (GAD), bifunctional oxirane (OXR), ethylene glycol diglycidyl ether (EGDE), and carbodiimides, such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). - Deconstruction Reagent
- The sequencing reaction involves binding of a
deconstruction reagent free end nucleic acid molecule nucleotides exonuclease exonuclease exonucleases Exonucleases - The skilled artisan will realize that enzymes with
exonuclease nucleotides nucleic acid molecule DNA nucleic acids polymerases - The skilled artisan will realize that the rate of
exonuclease nucleotides detection unit exonuclease reaction chamber exonuclease - Labels
- Certain embodiments may involve incorporating a label into the
nucleotides detection unit - Labels for use in embodiments involving Raman spectroscopy are discussed above. In other embodiments, the label moiety to be used may be a fluorophore, such as Alexa 350, Alexa 430, AMCA (7-amino-4-methylcoumarin-3-acetic acid), BODIPY (57-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid) 630/650, BODIPY 650/665, BODIPY-FL (fluorescein), BODIPY-R6G (6-carboxyrhodamine), BODIPY-TMR (tetramethylrhodamine), BODIPY-TRX (Texas Red-X), Cascade Blue, Cy2 (cyanine), Cy3, Cy5, 6-FAM (5-carboxyfluorescein), Fluorescein, 6-JOE (2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein), Oregon Green 488, Oregon Green 500, Oregon Green 514, Pacific Blue, Rhodamine Green, Rhodamine Red, ROX (6-carboxy-X-rhodamine), TAMRA (N,N,N′,N′-tetramethyl-6-carboxyrhodamine), Tetramethylrhodamine, and Texas Red. Fluorescent or luminescent labels can be obtained from standard commercial sources, such as Molecular Probes (Eugene, Oreg.).
- Reaction Chamber
- The
reaction chamber immobilization surface 14, 103,nucleic acid molecule deconstruction reagent nucleotides reaction chamber - In certain embodiments, the
reaction chamber flow path 12 or channels to provide connections to a waste port, to anucleic acid deconstruction reagent reaction chamber apparatus flow path 12, may be manufactured as a single integrated chip. Such a chip may be manufactured by methods known in the art, such as by photolithography and etching. However, the manufacturing method is not limiting and other methods known in the art may be used, such as laser ablation, injection molding, casting, or imprinting techniques. Methods for manufacture of nanoelectromechanical systems may be used for certain embodiments. (See, e.g., Craighead, Science 290: 1532-36, 2000.) Microfabricated chips are commercially available from sources such as Caliper Technologies Inc. (Mountain View, Calif.) and ACLARA BioSciences Inc. (Mountain View, Calif.). - In a non-limiting example, Borofloat glass wafers (Precision Glass & Optics, Santa Ana, Calif.) may be pre-etched for a short period in concentrated HF (hydrofluoric acid) and cleaned before deposition of an amorphous silicon sacrificial layer in a plasma-enhanced chemical vapor deposition (PECVD) system (PEII-A, Technics West, San Jose, Calif.). Wafers may be primed with hexamethyldisilazane (∈&IDS), spin-coated with photoresist (Shipley 1818, Marlborough, Mass.) and soft-baked. A contact mask aligner (Quintel Corp. San Jose, Calif.) may be used to expose the photoresist layer with one or more mask designs, and the exposed photoresist removed using a mixture of Microposit developer concentrate (Shipley) and water. Developed wafers may be hard-baked and the exposed amorphous silicon removed using CF4 (carbon tetrafluoride) plasma in a PECVD reactor. Wafers may be chemically etched with concentrated HF to produce the
reaction chamber path 12 and any channels. The remaining photoresist may be stripped and the amorphous silicon removed. - Access holes may be drilled into the etched wafers with a diamond drill bit (Crystalite, Westerville, Ohio). A finished chip may be prepared by thermally bonding an etched and drilled plate to a flat wafer of the same size in a programmable vacuum furnace (Centurion VPM, J. M. Ney, Yucaipa, Calif.). In certain embodiments, the chip may be prepared by bonding two etched plates to each other. Alternative exemplary methods for fabrication of a chip incorporating a
reaction chamber path 12 are disclosed in U.S. Pat. Nos. 5,867,266 and 6,214,246. - To facilitate detection of
nucleotides detection unit reaction chamber path 12 may be selected to be transparent to electromagnetic radiation at the excitation and emission frequencies used for thedetection unit reaction chamber path 12 that are opposite thedetection unit detection unit detection unit reaction chamber path 12 may contain a mesh comprising silver, gold, platinum, copper or aluminum. The skilled artisan will realize that in embodiments involving aflow path 12, thenucleotides 16 will generally be detected while they are in theflow path 12. In embodiments without aflow path 12, thenucleotides 104 will be detected in thereaction chamber 101. - In various embodiments, the
reaction chamber - Flow Path
- In certain embodiments, the
free nucleotides 16 are moved down aflow path 12 past thedetection unit 18. A non-limiting example of techniques for transport offree nucleotides 16 includes microfluidic techniques. Theflow path 12 can comprise a microcapillary (available, e.g., from ACLARA BioSciences Inc., Mountain View, Calif.) or a liquid integrated circuit (e.g., Caliper Technologies Inc., Mountain View, Calif.). Such microfluidic platforms require only nanoliter volumes of sample. - In certain embodiments, the
free nucleotides 16 to be detected move down theflow path 12 by bulk flow of solvent. In other embodiments, microcapillary electrophoresis may be used to transportfree nucleotides 16 down theflow path 12 and past thedetection unit 18. Microcapillary electrophoresis generally involves the use of a thin capillary or channel that may or may not be filled with a particular separation medium. Electrophoresis of appropriately charged molecular species, such as negatively chargednucleotides 16, occurs in response to an imposed electrical field, negative on thereaction chamber 11 side of the apparatus and positive on thedetection unit 18 side. Although electrophoresis is often used for size separation of a mixture of components that are simultaneously added to the microcapillary, it can also be used to transport similarlysized nucleotides 16 that are sequentially added to theflow path 12. Because the purine nucleotides (A, G) 16 are larger than the pyrimidine nucleotides (C, T, U) 16 and would therefore migrate more slowly, the length of theflow path 12 and corresponding transit time past thedetector unit 18 should be kept to a minimum to prevent differential migration from mixing up the order ofnucleotides 16 released from thenucleic acid 13. Alternatively, the separation medium filling the microcapillary may be selected so that the migration rates of purine andpyrimidine nucleotides 16 down theflow path 12 are similar or identical. Methods of microcapillary electrophoresis have been disclosed, for example, by Woolley and Mathies (Proc. Natl. Acad. Sci. USA 91:11348-352, 1994). - Microfabrication of microfluidic devices, including microcapillary electrophoretic devices has been discussed in, e.g., Jacobsen et al. (Anal. Biochem, 209:278-283, 1994); Effenhauser et al. (Anal. Chem. 662949-2953, 1994); Harrison et ol. (Science 261 :895-897, 1993) and U.S. Pat. No. 5,904,824. Typically, these methods comprise photolithographic etching of micron scale channels on silica, silicon or other crystalline substrates or chips, and can be readily adapted for use. In some embodiments, the microcapillary may be fabricated from the same polymeric materials described for the fabrication of the
reaction chamber - Detection Unit
- Embodiments Involving Raman Spectroscopy
- In some embodiments, the
detection unit nucleotides nucleotides - A non-limiting example of a
Raman detection unit excitation beam YAG laser sapphire laser Pulsed laser beams continuous laser beams excitation beam flow path 12 or thereaction chamber 101. The Raman emission light from thenucleotides Raman detector detector flow path 12 or thereaction chamber 101 to provide an increased signal due to surface enhanced Raman or surface enhanced Raman resonance. - Alternative embodiments of
detection units spectrophotometer excitation source ion laser ion laser 19, 108 (Innova 70, Coherent). -
Alternative excitation sources nitrogen laser 19, 108 (Laser Science Inc.) at 337 nm and a helium-cadmium laser 19, 108 (Liconox) at 325 nm (U.S. Pat. No. 6,174,677). Theexcitation beam flow path 12 orreaction chamber 101 using a 6× objective lens (Newport, Model L6X). The objective lens may be used to both excite thenucleotides excitation beam Alternative Raman detectors detectors - Any suitable form or configuration of Raman spectroscopy or related techniques known in the art may be used for detection of
nucleotides - Embodiments Involving FRET
- In certain alternative embodiments, the
nucleotides - Molecules that are frequently used as tags for FRET include fluorescein, 5-carboxyfluorescein (FAM), 2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein (JOE), rhodamine, 6-carboxyrhodamine (R6G), N,N,N′,N′-tetramethyl-6-carboxyrhodamine (TAMRA), 6-carboxy-X-rhodamine (ROX), 4-(4′-dimethylaminophenylazo) benzoic acid (DABCYL), and 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid (EDANS). Other potential FRET donor or acceptor molecules are known in the art (See U.S. Pat. No. 5,866,336, Table 1). The skilled artisan will be familiar with the selection of pairs of tag molecules for FRET (U.S. Pat. No. 5,866,336).
- In embodiments involving FRET, the donor and acceptor molecules may be covalently or non-covalently attached to various constituents of the
sequencing apparatus nucleotides exonuclease flow path 12. - In certain embodiments, the donor molecule may be attached to the
exonuclease flow path 12 and the acceptor molecules attached to thenucleotides nucleotide exonuclease path 12, although in other embodiments only a single donor molecule may be present. - Upon excitation, the donor molecules will transfer their energy to the acceptor tag molecules attached to the
nucleotides nucleotides exonuclease nucleotide exonuclease deconstruction reagent flow path 12 in the location where theexcitation beam 20 is focused, only nucleotides 16 within the focus of theexcitation beam 20 should give a detectable fluorescent signal. The wavelength of theexcitation beam nucleotide nucleic acid molecule - In certain embodiments, the template
nucleic acid excitation beam laser sensitive fluorescence detector - Information Processing and Control System and Data Analysis
- In certain embodiments, the nucleic
acid sequencing apparatus - The computer may further comprise a random access memory (RAM) or other dynamic storage device, a read only memory (ROM) or other static storage and a data storage device such as a magnetic disk or optical disc and its corresponding drive. The information processing system may also comprise other peripheral devices known in the art, such a display device (e.g., cathode ray tube or Liquid Crystal Display), an alphanumeric input device (e.g., keyboard), a cursor control device (e.g., mouse, trackball, or cursor direction keys) and a communication device (e.g., modem, network interface card, or interface device used for coupling to Ethernet, token ring, or other types of networks).
- In particular embodiments, the
detection unit detection unit standard nucleotides nucleotides reaction chamber 101 or theflow path 12 to identify the type ofnucleotide nucleic acid molecule nucleotides nucleic acid molecule detection unit nucleic acid - It should be noted that, while the processes described herein may be performed under the control of a programmed processor, in alternative embodiments, the processes may be fully or partially implemented by any programmable or hardcoded logic, such as Field Programmable Gate Arrays (FPGAs), ‘ITL logic, or Application Specific Integrated Circuits (ASICs), for example. Additionally, the disclosed methods may be performed by any combination of programmed general purpose computer components and/or custom hardware components.
- Following the data gathering operation, the data will typically be reported to a data analysis operation. To facilitate the analysis operation, the data obtained by the
detection unit detection unit - In certain embodiments, custom designed software packages may be used to analyze the data obtained from the
detection unit
Claims (13)
1-16. (canceled)
17. A method of sequencing nucleic acids comprising:
a. preparing a single nucleic acid molecule, one end of the nucleic acid molecule attached to an immobilization surface within a reaction chamber;
b. sequentially removing nucleotides from the unattached end of the nucleic acid molecule;
c. separating the nucleotides from the nucleic acid molecule; and
d. detecting the nucleotides by Raman spectroscopy.
18. The method of claim 17 , wherein the nucleotides are removed from the unattached end of the nucleic acid molecule by exonuclease activity.
19. The method of claim 17 , wherein a tag molecule is attached to each nucleotide after the nucleotide is removed from the nucleic acid molecule.
20. The method of claim 17 , wherein the nucleotides are detected by surface enhanced Raman scattering, surface enhanced resonance Raman scattering, stimulated Raman scattering, inverse Raman, stimulated gain Raman spectroscopy, hyper-Raman scattering or coherent anti-Stokes Raman scattering.
21. A method of sequencing nucleic acids comprising:
a. preparing a single nucleic acid molecule, one end of the nucleic acid molecule attached to an immobilization surface within a reaction chamber;
b. sequentially removing nucleotides from the unattached end of the nucleic acid molecule; and
c. quantifying the nucleotides in the reaction chamber by Raman spectroscopy.
22. The method of claim 21 , wherein the nucleotides are removed from the unattached end of the nucleic acid molecule by exonuclease activity.
23. The method of claim 21 , wherein a tag molecule is attached to each nucleotide after the nucleotide is removed from the nucleic acid molecule.
24. The method of claim 21 , wherein the nucleotides are detected by surface enhanced Raman scattering, surface enhanced resonance Raman scattering, stimulated Raman scattering, inverse Raman, stimulated gain Raman spectroscopy, hyper-Raman scattering or coherent anti-Stokes Raman scattering.
25. A method of sequencing nucleic acids comprising:
a. preparing a single nucleic acid molecule, one end of the nucleic acid molecule attached to an immobilization surface within a reaction chamber;
b. sequentially removing nucleotides from the unattached end of the nucleic acid molecule; and
c. detecting the nucleotides by fluorescence resonance energy transfer (FRET) spectroscopy.
26. The method of claim 25 , wherein the nucleotides are attached to acceptor molecules after they are removed from the nucleic acid molecule.
27. The method of claim 26 , wherein the nucleotides are removed by an exonuclease.
28. The method of claim 27 , wherein one or more donor molecules is attached to the exonuclease.
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Also Published As
Publication number | Publication date |
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AU2002323494A1 (en) | 2003-04-07 |
EP1770173A3 (en) | 2007-06-20 |
CN100360683C (en) | 2008-01-09 |
JP2005504282A (en) | 2005-02-10 |
EP1770173A2 (en) | 2007-04-04 |
KR20040035875A (en) | 2004-04-29 |
CN1556861A (en) | 2004-12-22 |
WO2003027326A2 (en) | 2003-04-03 |
US6982165B2 (en) | 2006-01-03 |
WO2003027326A3 (en) | 2003-12-11 |
US20030058799A1 (en) | 2003-03-27 |
KR100669589B1 (en) | 2007-01-15 |
EP1430149A2 (en) | 2004-06-23 |
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