Kit for luciferase assay

United States Patent m

Wood

[54] KIT FOR LUCIFERASE ASSAY

[75] Inventor: Keith V- Wood, Madison, Wis.

[73] Assignee: Promega Corporation, Madison, Wis.

[21] Appl. No.: 485,834
[22] Filed: Jun. 7,1995

Related U.S. Application Data

[62] Division of Ser. No. 189,558, Jan. 31, 1994, which is a division of Ser. No. 580,371, Sep. 10, 1990, Pat No. 5,283,179.

[51] Int CI.6 C12Q 1/66; G01N 33/53

[52] U.S. CI 435/8; 435/4; 435/184;

435/810; 435/968; 435/975; 436/164; 436/166;

436/172; 436/808

[58] Field of Search 435/8, 4, 184,

435/810, 968, 975; 436/164, 166, 172,

808

[56] References Cited

U.S. PATENT DOCUMENTS

4,234,681 11/1980 Deluca-McElroy 435/8

4,235,961 11/1980 Lundinetal 435/8

4,246,340 1/1981 Lundin et al 435/8

4,614,712 9/1986 Baldwin et al 435/4

4,665,022 5/1987 Schoeffer et al 435/8

4,833,075 5/1989 Vijayalakshmi et al 435/8

FOREIGN PATENT DOCUMENTS

0 299 601 1/1989 European Pat. Off..
0 309 184 3/1989 European Pat. Off..
2026156 1/1980 United Kingdom .

OTHER PUBLICATIONS

Webster et al, Biolumin. Chemilumin, 2nd, pp. 499-504, 1981.

Shimomura, Symp. Soc. Exp. Biol, vol. 39, pp. 351-372, 1985.

Airth et al., "The Function of Coenzyme A in Luminescence," Biochim. et Biophys. Acta 27, 519-532 (1958).

Rhodes and McElroy, "The Synthesis and Function of Luciferyl-Adenylate and Oxyluciferyl-Adenylate," J. Biol. Chem. 233, 1528-1537 (1958).

McElroy and Seliger, "The Chemistry of Light Emission," Advances Enzymol. 25, 119-166 (1963). Hammerstedt, "An Automated method for ATP analysis utilizing the luciferin-luciferase reaction," Anal. Biochem. 52, pp. 449^155 (1973).

Webster, et al., "Optimization of the Firefly Luciferase Assay for ATP," J. Appl. Biochem. 2, pp. 469^179 (1980). Sillero, et al., "Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase", Eur. J. Biochem, vol. 202, pp. 507-513 (1991).

Gunther et al, Eur. J. Biochem. vol. 202, No. 2, pp. 507-513, 1991.

Wood, Chemical Abstracts, vol. 117, p. 365, Ref. #1273631C, 1992 (Chemilumin. Proc. Int Symp. 6<h. 1990, 11-14).

Primary Examiner—Cecilia Tsang
Assistant Examiner—Abdel A. Mohamed
Attorney, Agent, or Firm—Foley & Lardner

[57] ABSTRACT

Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and lucif erin (D-(-)-2-(6'-hydroxy -2'-benzotMazolyl)-A2-thiazoline-4-carboxylic acid).

5 Claims, 8 Drawing Sheets