WO2017052054A1

WO2017052054A1 - Aligned nanofiber manufacturing device, membrane for eardrum or cornea regeneration, and nerve conduit made of nanofibers and manufacturing method therefor

Info

Publication number: WO2017052054A1
Application number: PCT/KR2016/007939
Authority: WO
Inventors: 김철생; 박찬희; 김정인; 황태인
Original assignee: 전북대학교산학협력단
Priority date: 2015-09-25
Filing date: 2016-07-21
Publication date: 2017-03-30

Abstract

The present invention relates to a device and method for manufacturing aligned nanofibers. Particularly, the present invention relates to: easier manufacturing of nanofibers, which are aligned in one direction, through electrospinning than a conventional method; a membrane for eardrum or cornea regeneration using the same; a nerve conduit which can easily connect cut nerve lines; and a method for manufacturing the nerve conduit.

Description

Arranged nanofiber manufacturing apparatus, membrane for regeneration of tympanic membrane or cornea, neural conduit consisting of nanofibers and method for manufacturing same

The present invention relates to an ordered nanofiber manufacturing apparatus and a method for manufacturing the same, and in particular, to easily manufacture a nanofiber aligned in one direction through electrospinning, using the membrane for regeneration of the tympanic membrane or cornea, and the cut neural wire easily The present invention relates to a neurological diagram capable of connecting and a method of manufacturing the same.

Fiber nanomaterial (NT), a nano-structured fiber new material technology, is a new technology that overcomes the limitations of existing fiber technology, and can create new technologies and new materials for future convergence in high-tech industries such as IT, NT, ET, and BT industries. It is a promising convergence new material technology.

As nanofibers have unique characteristics such as ultra-high surface area effect, nano-size effect, and ultra-molecular array effect, they are emerging as next generation high-performance high-tech new materials. In many fields, their applications are widening day by day.

Therefore, development of manufacturing process of nanofibers with diameter of nano-size, development of new nanofiber materials expressing new functions through precise nano-structure design controlled by nano-size on the inside, outside and surface of fiber The development of high-performance nanofiber-based fusion nanofiber materials through the control of such nano-level particles or structures is required.

Methods of manufacturing nanofibers include drawing, template synthesis, phase separation, self assembly, electrospinning, and the like. Electrospinning is generally applied as a method that can be produced.

Electrospinning method is to apply a high voltage between the nozzle (+ voltage) and the integrated electrode plate (-voltage) for spinning the polymer solution so that when the electric field larger than the surface tension of the polymer solution is formed in the form of nanofibers through the nozzle will be.

Nanofibers produced by electrospinning method have properties of polymer solution, molecular structure, viscosity, elasticity, conductivity, dielectric property, material properties such as polarity and surface tension, electric field strength, distance between nozzle and integrated electrode, supply of polymer solution. It is greatly affected by radiation conditions such as speed and temperature.

In addition, the nanofibers manufactured by the conventional electrospinning apparatus are randomly distributed or network structure because the nanofibers radiated from the nozzle are irregularly sprayed.

If the nanofibers of the ordered structure can be obtained than the conventional nanofibers having an irregular structure, its utilization becomes much wider.

Recently, devices for producing aligned nanofibers have been developed.

However, the device developed so far to align the nanofibers in one direction has a disadvantage in that the structure is complicated and the purchase cost must be purchased separately from the conventional nanofiber manufacturing apparatus.

On the other hand, traumatic tympanic perforation is a disease that directly damages the tympanic membrane or causes perforation of the eardrum due to sudden pressure changes in the ear canal and middle ear.

The perforation of the tympanic membrane depends on the degree of damage to the tympanic membrane. If the tympanic membrane is about 40% of the total, it can be naturally regenerated.

Immediately after the injury, deafness and tinnitus appear, severe bleeding from the damaged eardrum can cause blood to bleed out of the ear canal, and severe pain can occur.

Perforated eardrums can be treated with paper patches or tympanic surgery, but paper patches are inadequate in color and less flexible, and surgery requires a sterile environment.

Techniques for implanting injured tympanic membranes using polymers considering biocompatibility have been developed.

The scaffold used as a reconstructive patch of damaged tympanic membrane should mimic the tympanic membrane to facilitate the movement of tympanic cells to increase the regeneration rate and have transparency to observe the progress of tympanic regeneration during the treatment period. There is no suitable membrane for regeneration of the tympanic membrane.

In addition, the cornea is damaged due to severe injury or disease, and surgery is often performed.

Since corneal endothelium is nearly impossible to regenerate once damaged, various methods have been tried in histology for the treatment of damaged cornea.

PLGA, PCL, silk and the like are widely used as biocompatible support materials for tissue regeneration.

The support used for artificial cornea or corneal damage is a porous support made of a biodegradable polymer for regenerating eye tissue at the damaged eye area by healing the damaged eye tissue by embedding in the damaged eye area.

The scaffold used for the damaged eye area is porous and the movement of corneal cells should be smoothed to increase the regeneration rate, and in order to have no discomfort during the treatment period, it must have transparency.

On the other hand, the nerves of the limbs cut by accidents or surgery are difficult to connect unless the cuts are tightly closed.

However, if the cuts are more than 5mm apart, direct closure is impossible, and in this case, there is no method other than autologous nerve transplantation, but there are disadvantages such as the use of one's own nerves for autografts, and limitations on the collectible nerves. .

Therefore, when a nerve defect is large, a method of using a nerve conduit has been studied as a method for restoring its function.

Neural conduit refers to a tube that fixes both ends of a cut nerve in an artificial tube and induces nerve connections into the tube.

These nerve conduits can prevent the penetration of scar tissue that interferes with nerve regeneration, induce the growth of axons in the right direction, and the substances that interfere with regeneration while retaining the regeneration materials secreted by the nerve itself It has the advantage of being cut off from the outside.

As the first neural conduit, a non-degradable silicone tube was used.

However, there is a problem that the nerve remains in the body even after the nerve is regenerated, causing chronic pain, calciumation of silicon, and pain caused by squeezing the regenerated nerve, and the trouble of removing the tube by reoperation after the nerve is completely repaired.

Therefore, recently, a neural conduit using polyglycolic acid (PGA), which is a biodegradable polymer material that melts in vivo, has been developed and applied to clinical practice.

In addition, Korean Patent No. 10-1541999 discloses a technique for manufacturing a neural conduit of a nanofiber tube by electrospinning a polymer solution.

However, since the conventional neural conduit using nanofibers is irradiated irregularly when the polymer solution is electrospun, the nanofibers forming the neural conduit are not aligned so that there is a limit to rapidly regenerating the cut nerves.

The present invention is to solve the above problems, it is possible to easily manufacture the aligned nanofibers using a simple structure, and also easy and inexpensive alignment by adding an additional configuration to the conventional commonly used nanofiber manufacturing apparatus It is an object of the present invention to provide an ordered nanofiber manufacturing apparatus capable of producing nanofibers.

In addition, the present invention is to solve the above-described problems, it is made of aligned nanofibers to facilitate the movement of damaged tympanocytes or corneal cells to increase the regeneration rate, has a transparency can observe the progress of tympanic regeneration It is an object of the present invention to provide a membrane for cornea regeneration that is suitable for corneal regeneration.

In addition, the present invention is to solve the above-mentioned problems, nanofibers that can easily produce a neural conduit connecting the cleaved neural wire and can be quickly made to regenerate the neural wire cut through the manufactured neural conduit The purpose is to provide a neural conduit consisting of and a method of manufacturing the same.

In order to achieve the above object, the ordered nanofiber manufacturing apparatus of the present invention comprises: an apparatus for producing nanofibers through electrospinning, comprising: a nozzle for electrospinning a polymer; A collector base of a conductive material in which the polymer radiated from the nozzle is integrated; A power supply unit supplying power having different polarities to the nozzle and the collector base; A first alignment member made of a conductive material coupled to a surface of the collector base; It comprises a second alignment member of a non-conductive material coupled to cover the upper surface of the first alignment member, the first alignment member is electrically connected to the collector base, the total area of the first alignment member The total area of the second alignment member covering the upper surface of the first alignment member is smaller than the total area of the collector base, and the total area of the second alignment member is smaller than the total area of the collector base. Or it is attached to the collector base.

The first alignment member has a lower strip shape in one direction and is coupled to the collector base, and the second alignment member has an upper surface of the collector base to which the first alignment member is not coupled and an upper surface of the first alignment member. Cover them together.

The second alignment member is formed in a strip shape long formed in one direction.

The second alignment member is disposed long in the same direction as the longitudinal direction of the first alignment member.

The second alignment member is disposed long in a direction perpendicular to the longitudinal direction of the first alignment member.

The first alignment member is composed of a plurality of spaced apart from each other, the second alignment member covers a plurality of the first alignment member.

An adhesive sheet of a non-conductive material is coupled to the surface of the collector base, and the first alignment member and the second alignment member are coupled to an upper surface of the attachment sheet, and the polymer electrospun from the nozzle is the second alignment member or It is attached to the attachment sheet.

At this time, both ends of the first alignment member are electrically connected to the collector base while surrounding both ends of the attachment sheet.

Preferably, the first alignment member is made of copper wire, and the second alignment member is made of cellophane tape.

In addition, the polymer electrospun from the nozzle is attached in a state aligned in one direction on the upper surface of the second alignment member to form a membrane for regeneration of the tympanic membrane or cornea having transparency.

The second alignment member is formed in a circular shape, the membrane for regeneration of the tympanic membrane or cornea, in which the nanofibers are aligned in one direction, is formed in a circular shape on an upper surface of the second alignment member.

A base layer of a non-conductive material made of a circular shape detachably coupled to the upper surface of the second alignment member; and made of a metal member of the conductive material is coupled to the center of the base layer, the electrical The spun polymer is aligned in one direction with respect to the metal member on the upper surface of the base layer to form a radial tympanic membrane or corneal regeneration membrane.

The second alignment member has a dome shape and protrudes from the center thereof.

In order to achieve the above object, the membrane for regeneration of the eardrum or cornea of the present invention is characterized in that the electrospun polymer nanofibers are arranged in one direction and have transparency.

The electrospun nanofibers are aligned in one direction toward the center to form a radial structure.

The electrospun nanofibers have a dome shape with a central convex shape.

In order to achieve the above object, the neural conduit made of the nanofibers of the present invention is connected to a neural wire cut at both ends thereof, and in the neural conduit of a tube shape connecting the neural wires, a plurality of electrospun nanofibers are aligned in one direction And arranged to form an inner surface of the neural conduit having a tubular shape; A plurality of electrospun nanofibers are arranged in an unaligned manner, characterized in that the non-aligned portion is formed on the outer peripheral surface of the alignment portion to form the outer surface of the neural conduit having a tubular shape.

The alignment portion has a long alignment of a plurality of nanofibers in the arrangement direction of the cut nerve wire.

The alignment unit is coupled to surround the outer peripheral surface of the cut nerve wire.

The alignment unit may include a measurement unit arranged inside the non-alignment unit; It is made of a coupling alignment portion that is wrapped around the outer peripheral surface of the neural wire, the non-alignment portion does not surround the outer peripheral surface of the coupling alignment portion so as to surround only the outer peripheral surface of the inner measurement alignment portion.

The alignment portion and the non-alignment portion are made of mats integrally connected in a horizontal direction on the same plane, and the mat is wound in a tube shape so that the alignment portion is disposed inside the non-alignment portion.

The mat, the alignment unit; An alignment unit which is integrally connected to the alignment unit in a horizontal direction on the same plane, and the alignment unit includes an internal measurement alignment unit connected to one side of the alignment unit; It consists of a coupling alignment portion connected to the upper and lower portions of the internal alignment line and the non-alignment, respectively, while the measurement line is wound from the internal alignment line forms the inner surface of the neural conduit having a tubular shape and the non-aligned portion is tubular Forming an outer side surface of the neural conduit, and the coupling alignment is disposed at both ends of the neural conduit having a tube shape.

The pores formed in the disalignment portion forming the outer surface of the neural conduit having a tube shape are sized to transmit nutrients and oxygen and to block the cells that make the wound.

In addition, in order to achieve the above object, the method of manufacturing a neural conduit made of nanofibers of the present invention is a mat consisting of an alignment portion formed by aligning nanofibers and an unalignment portion formed by non-aligning nanofibers by electrospinning. Mat-forming step of forming integrally connected in the horizontal direction on the; And a tube forming step of winding the mat to form a tube shape in which the alignment portion is disposed on the inner side and the non-alignment portion is disposed on the outer side.

In the tube forming step, the mat is wound so that the nanofibers constituting the alignment portion are aligned in the longitudinal direction of the tubular neural conduit formed.

According to the present invention as described above has the following advantages.

By using the simple structure of the first alignment member and the second alignment member can be easily produced aligned nanofibers.

In particular, by adding a first alignment member and a second alignment member, which are additional components to a conventionally used nanofiber manufacturing apparatus, it is possible to produce nanofibers easily and inexpensively aligned.

Since the nanofibers are aligned in one direction, the regeneration rate can be increased by smoothing the movement of damaged tympanocytes or corneal cells.

And it has transparency and can observe the progress of tympanic membrane regeneration, and has the effect suitable for corneal regeneration.

In addition, since the central portion is formed convexly, it can be made to have the same shape as the actual cornea.

Since the neural conduit of the present invention has an alignment portion in which the nanofibers are aligned in one direction, the neural catheter can be quickly regenerated through the neural regeneration of the neural catheter.

In addition, it is possible to easily prepare a neural conduit connecting the nerve wire cut by the production method of the present invention.

1 is a perspective view of a nanofiber manufacturing apparatus according to a first embodiment of the present invention,

FIG. 2 is a cross-sectional view taken along line AA ′ of FIG. 1;

3 is a perspective view schematically showing a state in which nanofibers are attached by a nanofiber manufacturing apparatus according to a first embodiment of the present invention;

4 is a photograph of the nanofibers according to the first embodiment of the present invention;

FIG. 5A is an enlarged image of the unordered nanofibers, that is, the non-alignment unit in FIG. 4, and FIG. 5B is an enlarged image of the nanofibers, ie the alignment unit, aligned in FIG. 4.

6 is a perspective view of a nanofiber manufacturing apparatus according to another first embodiment of the present invention;

7 is a perspective view of a nanofiber manufacturing apparatus according to a second embodiment of the present invention;

8 is a cross-sectional view taken along line B-B 'of FIG.

9 is a perspective view schematically showing a state in which nanofibers are attached by a nanofiber manufacturing apparatus according to a second embodiment of the present invention;

10 is an enlarged view illustrating an enlarged main part of a nanofiber manufacturing apparatus according to another second embodiment of the present invention;

11 is an enlarged view illustrating an enlarged main part of a nanofiber manufacturing apparatus according to the present invention or another embodiment;

12A and 12B are plan views of a tympanic membrane or regeneration membrane according to a second embodiment of the present invention.

13 is a perspective view of a state in which a nerve conduit made of nanofibers according to a third embodiment of the present invention is coupled to a neural wire;

14 is a cross-sectional view taken along line C-C 'of FIG. 13;

15a to 15c is a process diagram of a method of manufacturing a tubular neural conduit according to a third embodiment of the present invention.

제1실시예First embodiment

1 is a perspective view of a nanofiber manufacturing apparatus according to a first embodiment of the present invention, Figure 2 is a cross-sectional view taken along the line A-A 'of Figure 1, Figure 3 is a nano according to the first embodiment of the present invention A perspective view schematically showing a state in which nanofibers are attached by a fiber manufacturing apparatus.

The aligned nanofiber manufacturing apparatus of the present invention relates to an apparatus for producing nanofibers by electrospinning a polymer, as shown in Figs. 1 and 2, the nozzle 110, the collector base 120, and the power source. It comprises a supply unit 130, the first alignment member 140, and the second alignment member 150.

The nozzle 110 is to electrospin the polymer for producing nanofibers, it is sufficient to use a conventionally known electrospinning nozzle 110, a detailed description thereof will be omitted.

The collector base 120 is made of a conductive material as a part in which the polymer radiated from the nozzle 110 is integrated into nanofibers.

The collector base 120 is made of aluminum or the like.

In the first embodiment, the collector base 120 is formed in a cylindrical shape, but the collector base 120 may be formed in a flat shape instead of a cylindrical shape.

When the collector base 120 has a cylindrical shape, electrospinning may be performed while rotating the collector base 120 at a low speed of about 50 rpm instead of a high speed.

In addition, when the collector base 120 has a flat plate shape, the collector base 120 or the nozzle 110 may be electrospun while horizontally moving.

By rotating the collector base 120 or horizontally moving the collector base 120 or the nozzle 110 as described above, a large-area nanofiber can be obtained.

The power supply unit 130 supplies power of different polarities to the nozzle 110 and the collector base 120, and an anode of the power supply unit 130 is connected to the nozzle 110 and a cathode of the power supply unit 130 is connected to the nozzle 110. It is connected to the collector base 120.

The first alignment member 140 is made of a conductive material and is coupled to the surface of the collector base 120.

The first alignment member 140 is electrically connected to the collector base 120.

The total area of the first alignment member 140 coupled to the collector base 120 is smaller than the total area of the collector base 120.

In addition, the first alignment member 140 is formed in the form of a long strip in one direction and is coupled to the surface of the collector base 120.

In the first embodiment, the first alignment member 140 is coupled to the surface of the collector base 120 along the axial length direction of the collector base 120 having a cylindrical shape.

In some cases, the first alignment member 140 may be coupled in a circumferential direction perpendicular to the axial length direction of the collector base 120 having a cylindrical shape.

If the collector base 120 has a flat plate shape, the first alignment member 140 may be coupled to be disposed in various directions.

The first alignment member 140 may be made of a material that is good for electricity, and preferably made of copper wire.

More specifically, the first alignment member 140 is formed by cutting a flat copper plate having a thin thickness and a wide surface into a narrow strip shape.

The second alignment member 150 is made of a non-conductive material and is coupled while covering the top surface of the first alignment member 140.

The second alignment member 150 is preferably made of a cellophane tape having a non-conductive and self-coupling force.

The total area of the second alignment member 150 covering the upper surface of the first alignment member 140 is smaller than the total area of the collector base 120.

As described above, the polymer electrospun from the nozzle 110 by the second alignment member 150 is attached to the second alignment member 150 and / or the collector base 120.

The second alignment member 150 covers not only the top surface of the first alignment member 140 but also the top surface of the collector base 120 to which the first alignment member 140 is not coupled.

The second alignment member 150 is formed in a strip shape long formed in one direction.

The second alignment member 150 may be disposed to be elongated in the same direction as the longitudinal direction of the first alignment member 140, and the second alignment member 150 may be in the longitudinal direction of the first alignment member 140. It may be arranged long in the direction perpendicular to the.

When the second alignment member 150 is disposed in the same direction as the longitudinal direction of the first alignment member 140, as shown in FIG. 3, the nanofibers attached to the second alignment member 150. That is, the alignment unit 171 is aligned and attached in the vertical direction of the first alignment member 140.

In addition, when the second alignment member 150 is elongated in a direction perpendicular to the longitudinal direction of the second alignment member 150, the second alignment member 150 may be attached to the second alignment member 150 as illustrated in FIG. 3. Nanofibers aligned in a direction parallel to the longitudinal direction of the first alignment member 140, that is, the alignment portion 171 is attached, and are not aligned in a portion where the second alignment member 150 is absent. ) Nanofibers, that is, the non-alignment portion 172 is attached.

It is preferable that the first alignment member 140 is formed in plural and spaced apart from each other as in the first embodiment, and the second alignment member 150 includes a plurality of first alignment members 140. Cover it.

4 is a photograph of a nanofiber formed by electrospinning according to the first embodiment of the present invention, and FIGS. 5A and 5B are enlarged SEM photographs of the nanofiber shown in FIG. 4.

The photo shown in FIG. 4 is taken with the nanofibers attached to the top surface of the second alignment member 150 made of cellophane tape and electrospun together on the top surface of the collector base 120.

4 shows the aligned nanofiber portion, that is, the alignment portion 171, and the unaligned nanofiber portion, that is, the alignment portion 172, Random.

5A is an enlarged image of an unaligned part 172 attached to an upper surface of the collector base 120 which is not covered with the second alignment member 150.

5B is an enlarged image of the alignment unit 171 attached to the second alignment member 150.

As can be seen in FIGS. 5A and 5B, the nanofibers attached to the upper surface of the second alignment member 150, that is, the alignment unit 171, are nanofibers attached to a portion without the second alignment member 150. Compared with the non-alignment unit 172, it can be seen that the alignment is clearly in one direction.

The nanofibers formed by electrospinning as described above may be more easily aligned by the first alignment member 140 and the second alignment member 150, and may also be formed by the first alignment member 140 and the second alignment. The direction in which the nanofibers are aligned can also be easily adjusted according to the arrangement direction of the member 150.

The nanofibers are aligned in one direction so that the alignment portion 171 has a high transparency, and the non-alignment portion 172 is opaque.

Therefore, it is possible to produce a transparent mat using the alignment unit 171, it can be utilized as a nerve conduit for peripheral nerve regeneration.

In addition, when the nanofibers aligned in one direction are rotated about 90 degrees horizontally and then electrospun again to form the aligned nanofibers, nanofiber mats arranged in a substantially lattice pattern may be obtained.

The lattice-aligned nanofiber mat can adjust the pore size, which is a space between the nanofiber strands, and can be usefully used for the production of neural conduits through the growth of nerve cells.

Meanwhile, the present invention may combine the attachment sheet 160 of a non-conductive material such as paper on the surface of the collector base 120 as shown in FIG.

In this case, the first alignment member 140 and the second alignment member 150 are coupled to an upper surface of the attachment sheet 160, and the polymer that is electrospun from the nozzle 110 is the second alignment member 150. And / or attached to the attachment sheet 160.

The first alignment member 140 is coupled to the upper surface of the attachment sheet 160 and both ends thereof are electrically connected to the collector base 120.

More specifically, the first alignment member 140 coupled to the upper surface of the attachment sheet 160 is bent at both ends of the attachment sheet 160 while the lower portion bent while wrapping both ends of the attachment sheet 160 to the collector base 120. To be electrically connected to the

The second alignment member 150 is coupled to the top surface of the attachment sheet 160 while covering the top surface of the first alignment member 140.

By combining the attachment sheet 160 on the surface of the collector base 120 as described above, it is possible to implement a nanofiber mat attached to the surface of the attachment sheet 160.

제2실시예Second embodiment

Figure 7 is a perspective view of a nanofiber manufacturing apparatus according to a second embodiment of the present invention, Figure 8 is a cross-sectional view taken along line B-B 'of Figure 7, Figure 9 is a nano according to a second embodiment of the present invention 10 is a perspective view schematically showing a state in which nanofibers are attached by a fiber manufacturing apparatus, and FIG. 10 is an enlarged view illustrating an enlarged main part of a nanofiber manufacturing apparatus according to another second embodiment of the present invention, and FIG. 12A and 12B are enlarged views of the main part of the nanofiber manufacturing apparatus according to the second embodiment of the present invention, and FIGS. 12A and 12B are plan views of the membrane for regeneration or regeneration according to the second embodiment of the present invention.

The nanofiber manufacturing apparatus of the second embodiment is for producing a membrane for regeneration of the tympanic membrane or cornea, and as shown in FIGS. 7 to 9, the nozzle 210, the collector base 220, and the power supply 230. And a first alignment member 240 and a second alignment member 250.

Compared to the first embodiment, the second embodiment has a difference in that there are additional matters with respect to the second alignment member 250, which will be described below, and description of the rest of the configuration will be omitted.

The polymer to be electrospun in the second embodiment is selected from the group consisting of synthetic polymers such as polyglycolic acid, polylactic acid, polycaprolactone and copolymers thereof, or natural polymers such as collagen, hyaluronic acid and chitosan and mixtures thereof. It is made of a polymer.

In addition, the polymer is preferably made of a biodegradable polymer.

The second alignment member 250 may be formed in a quadrangular shape as in the first embodiment, and may be formed in a shape, that is, a circular shape, specialized for the manufacture of the membrane for regeneration of the tympanic membrane or cornea.

When the second alignment member 250 is formed in a circular shape, the shape of the nanofibers attached to the upper surface of the second alignment member 250 is formed in a circle similar to the shape of the eardrum or cornea, and thus is easy to manufacture and use. It works.

When electrospinning as described in the first embodiment, the nanofibers, that is, the alignment part 271, are arranged on the top surface of the second alignment member 250, as shown in FIG. When placed in the corneal region, damaged eardrum cells or corneal cells are regenerated along the nanofibers aligned in one direction, thereby increasing the regeneration rate of the eardrum cells and corneal cells than the unaligned nanofibers.

In addition, when the electrospun nanofibers are aligned in one direction, it is possible to manufacture a mat having transparency, and when used as a membrane for regeneration of a tympanic membrane, medical personnel can easily check the regeneration of the tympanum through a transparent membrane. It can be suitably used as a membrane for corneal regeneration that requires.

On the other hand, the nanofiber manufacturing apparatus of the present invention may be made of a structure as shown in FIG.

FIG. 10 is an enlarged view of a portion of the circular second alignment member 250 of FIG. 7.

The manufacturing apparatus shown in FIG. 10 further includes a base layer 266.

The base layer 266 has a circular shape and is detachably coupled to an upper surface of the second alignment member 250 and is made of a non-conductive material.

The base layer 266 is preferably made of a plate-like quartz or the like.

In addition, a metal member 267 of a conductive material is coupled to the center of the base layer 266.

As a result, when the polymer is electrospun through the nozzle 210, unlike the linear nanofiber structure illustrated in FIG. 12A, the base layer 266 on which the metal member 267 is located is illustrated in FIG. 12B. A radially aligned membrane is formed around the center of the beam in one direction.

As the nanofibers are formed radially as described above, when used as a membrane for regeneration of the tympanic membrane, there is an effect of increasing the regeneration rate of the tympanic membrane.

In addition, the nanofiber manufacturing apparatus of the present invention may be made of a structure as shown in FIG.

FIG. 11 is an enlarged view of a portion of the circular second alignment member 250 of FIG. 7.

In the manufacturing apparatus illustrated in FIG. 11, the second alignment member 250 is formed in a dome shape so that a center portion thereof protrudes convexly.

As a result, when the polymer is electrospun through the nozzle 210, a dome-shaped membrane having a convex center can be formed on the upper surface of the second alignment member 250.

As described above, the membrane is formed in a convex dome shape, and thus has a shape substantially the same as that of the actual cornea, and the regeneration of the corneal cells can be performed quickly, and at the same time, the patient can feel like a real cornea.

제3실시예Third embodiment

FIG. 13 is a perspective view of a state in which a neural conduit made of nanofibers is coupled to a neural wire, and FIG. 14 is a cross-sectional view taken along line C-C ′ of FIG. 13, and FIGS. 15A to 15C. Is a process diagram of a method of manufacturing a tubular neural conduit according to a third embodiment of the present invention.

The present invention relates to a tube-shaped neural conduit connected to nerve wires cut at both ends thereof to connect neural wires, as shown in FIGS. 13 to 15, as an alignment part 310 and an unalignment part 320. Is done.

As shown in FIGS. 4 and 5b described in the first embodiment, the alignment unit 310 has a plurality of electrospun nanofibers arranged in one direction and have transparency, and a neural conduit A having a tube shape. To form an inner surface.

As shown in FIGS. 4 and 5A described in the first embodiment, the non-alignment unit 320 is disposed on an outer circumferential surface of the alignment unit 310 in a non-aligned and opaque manner. To form an outer surface of the neural conduit A. FIG.

That is, in the tubular neural conduit A, the alignment part 310 is disposed inside, and the non-alignment part 320 is disposed outside.

The alignment unit 310 is formed by aligning a plurality of nanofibers in one direction, and a plurality of nanofibers are long aligned in the same direction as the arrangement direction of the cut nerve wires 330.

Therefore, the aligned nanofibers forming the alignment unit 310 are interconnected to the cut neural wire 330.

At this time, the alignment unit 310 is coupled to surround the outer peripheral surface of the cut nerve wire 330.

The alignment unit 310 as described above is composed of the internal alignment unit 311 and the coupling alignment unit 312.

The internal measurement alignment unit 311 is disposed inside the nonalignment unit 320.

The coupling alignment unit 312 is coupled to surround the outer circumferential surface of the neural wire 330.

The inner measurement alignment unit 311 and the coupling alignment unit 312 are nanofibers are arranged in the same direction and are integrally connected.

The non-alignment unit 320 does not surround the outer circumferential surface of the coupling alignment unit 312 so as to surround only the outer circumferential surface of the inner measurement alignment unit 311.

The alignment unit 310 and the non-alignment unit 320 as described above may be manufactured separately and coupled to the inside and the outside, respectively.

However, as shown in the present invention, the alignment unit 310 and the non-alignment unit 320 are integrally connected in the horizontal direction on the same plane to form a flat mat (M).

As shown in FIGS. 15A and 4, the alignment parts 310 and the random parts 320 are integrally connected to one side thereof and are arranged flat on the same plane.

As described above, in the mat M in which the alignment unit 310 and the non-alignment unit 320 are integrally formed on the same plane, the internal measurement alignment unit 311 is the non-alignment unit 320 as shown in FIG. 15A. It is connected to one side of.

The coupling alignment unit 312 is connected to the upper and lower portions of the internal measurement alignment unit 311 and the non-alignment unit 320, respectively.

Accordingly, the alignment unit 310 is formed in a substantially 'c' shape by the internal measurement alignment unit 311 and the coupling alignment unit 312, and the non-alignment unit 320 is inside the alignment unit 310. Is placed on.

As described above, a technique of integrally forming the alignment unit 310 and the non-alignment unit 320 on the same plane may be performed using the manufacturing apparatus of the first embodiment described above.

More specifically, the method for producing a neural conduit of the present invention, the mat forming step and tube forming step.

The mat forming step is a step of forming a mat (M) consisting of the alignment portion 310 and the non-alignment portion 320 in the shape as shown in Figure 15a by electrospinning.

At this time, the alignment unit 310 and the non-alignment unit 320 are integrally connected in the horizontal direction on the same plane to form the mat (M).

In the tube forming step, as illustrated in FIGS. 15B and 15C, the mat M having a flat plate shape is wound so that the alignment unit 310 is disposed on the inner side and the non-alignment unit 320 is disposed on the outer side. Forming a tube shape.

As a result, the alignment unit 310 is wound while being disposed inside the non-alignment unit 320.

Specifically, when wound from the internal measurement liner 311, the internal measurement liner 311 forms an inner surface of the neural conduit A having a tube shape, and the non-aligned part 320 has a neural conduit having a tube shape. Forming the outer surface of (A) and the coupling alignment portion 312 is disposed at both ends of the neural conduit (A) having a tube shape.

In the tube forming step, when the mat (M) is wound to form a tube shape, the nanofibers constituting the alignment unit 310 in the longitudinal direction of the neural conduit A having a tube shape are aligned so as to be long aligned. Wind up mat (M).

By making the inner measurement column portion 311 made of aligned nanofibers as described above, the cut nerve line 330 can be interconnected while growing rapidly along the inner measurement column portion 311.

In addition, since the coupling alignment unit 312 coupled to the neural wire 330 is formed of aligned nanofibers, the coupling alignment unit 312 and the neural wire 330 have high transmittance due to the characteristics of the aligned nanofibers. Surgery to connect can be made easily.

In addition, the outer surface of the nerve conduit (A) is made of the non-aligning portion 320, while the nutrients and oxygen for the neural wire 330 to sleep well, while allowing the transmission of the inside of the nerve conduit (A), wound Cells that make up can be blocked.

To this end, the pores formed in the non-aligning portion 320 forming the outer surface of the neural conduit (A), the nutrients and oxygen are permeable to have a size that blocks the cells that make the wound.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the technical idea of the present invention.

Since the nanofibers produced by the present invention are manufactured in an aligned state in one direction, the nanofibers may be used for corneal regeneration membranes, membrane regeneration membranes, neural conduits and the like.

Claims

In the device for producing nanofibers by electrospinning,

A nozzle for electrospinning the polymer;

A collector base of a conductive material in which the polymer radiated from the nozzle is integrated;

A power supply unit supplying power having different polarities to the nozzle and the collector base;

A first alignment member made of a conductive material coupled to a surface of the collector base;

It comprises a second alignment member of the non-conductive material coupled to cover the upper surface of the first alignment member,

The first alignment member is electrically connected to the collector base,

The total area of the first alignment member is smaller than the total area of the collector base,

The total area of the second alignment member covering the upper surface of the first alignment member is smaller than the total area of the collector base,

The nanofiber manufacturing apparatus, characterized in that the polymer is electrospun from the nozzle is attached to the second alignment member or the collector base.
The method according to claim 1,

The first alignment member is formed in a strip shape long in one direction is coupled to the collector base,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that for covering the top surface of the collector and the first alignment member is not coupled to the first alignment member.
The method according to claim 2,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that consisting of a strip form long formed in one direction.
The method according to claim 3,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that the elongated arrangement in the same direction as the longitudinal direction of the first alignment member.
The method according to claim 3,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that the elongated arrangement in the direction perpendicular to the longitudinal direction of the first alignment member.
The method according to claim 5,

The first alignment member is composed of a plurality of spaced apart from each other,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that for covering the plurality of the first alignment member.
The method according to claim 1,

The surface of the collector base is bonded to the non-conductive material attachment sheet,

Both ends are electrically connected to the collector base while the first alignment member is coupled to the upper surface of the attachment sheet.

The second alignment member is coupled to the top surface of the attachment sheet while covering the top surface of the first alignment member,

The nanofiber manufacturing apparatus, characterized in that the polymer electrospun from the nozzle is attached to the second alignment member or the attachment sheet.
The method according to claim 7,

Both ends of the first alignment member is bent and wrapped around both ends of the attachment sheet, the bent bottom is aligned nanofiber manufacturing apparatus, characterized in that electrically connected to the collector base.
The method according to claim 1,

The first alignment member is made of a copper wire,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that made of cellophane tape.
The method according to claim 1,

Electrospun polymer from the nozzle is attached to the aligned state in one direction on the upper surface of the second alignment member aligned nanofiber manufacturing apparatus, characterized in that to form a membrane for regeneration or cornea having a transparency.
The method according to claim 10,

The second alignment member is made of a circle,

The nanofibers are aligned in one direction, the membrane for regeneration of the tympanic membrane or cornea is aligned nanofiber manufacturing apparatus, characterized in that formed in a circular shape on the upper surface of the second alignment member.
The method according to claim 10,

A base layer of a non-conductive material made of a circular shape detachably coupled to the upper surface of the second alignment member;

The center of the base layer is a metal member of the conductive material is coupled,

The electrospun polymer in the nozzle is aligned in one direction around the metal member on the upper surface of the base layer aligned nanofiber manufacturing apparatus, characterized in that to form a radial membrane or corneal regeneration membrane.
The method according to claim 10,

The second alignment member is aligned nanofiber manufacturing apparatus, characterized in that the central portion is formed convexly protruding in the shape of a dome.
Membrane for cornea or corneal regeneration, characterized in that the electrospun polymer nanofibers are aligned in one direction and have transparency.
The method according to claim 14,

The electrospun nanofibers membranes for tympanic or corneal regeneration, characterized in that formed in a radial structure aligned in one direction toward the center.
The method according to claim 14,

The electrospun nanofibers are membranes for retina or cornea regeneration, characterized in that the central portion is made of a convex shape.
In the tube-shaped neural conduit connected to both ends of the nerve wire is connected to the nerve wire,

A plurality of electrospun nanofibers arranged in one direction and arranged to form an inner surface of the neural conduit having a tubular shape;

A plurality of electrospun nanofibers are arranged unaligned, the neural conduit made of nanofibers, characterized in that the non-aligned portion is formed on the outer peripheral surface of the alignment portion to form the outer surface of the neural conduit having a tube shape.
The method according to claim 17,

The alignment unit is a nerve conduit made of nanofibers, characterized in that the plurality of nanofibers are arranged long in the arrangement direction of the cut neural wire.
The method according to claim 17,

The alignment unit is a nerve conduit made of nanofibers, characterized in that coupled to wrap around the outer peripheral surface of the cut nerve line.
The method of claim 19,

The alignment unit,

An internal measurement alignment unit disposed inside the non-alignment unit;

Consists of a coupling alignment unit is wrapped around the outer peripheral surface of the neural wire,

The non-aligned portion does not surround the outer peripheral surface of the coupling alignment portion of the nerve conduit made of nanofibers, characterized in that it wraps only the outer peripheral surface of the inner measurement alignment portion.
The method according to claim 17,

The alignment portion and the non-alignment portion is made of a mat integrally connected in the horizontal direction on the same plane,

The mat is wound in a tube shape of the nerve conduit made of nanofibers, characterized in that the alignment portion is disposed inside the non-alignment portion.
The method of claim 20,

The mat,

The alignment unit;

Consists of an alignment unit which is integrally connected in the horizontal direction on the same plane as the alignment unit,

The alignment unit,

An internal measuring alignment unit connected to one side of the non-aligning unit;

Consists of a coupling alignment unit connected to the upper and lower portions of the measurement and alignment unit, respectively,

Winding from the measurement line portion, the measurement line portion forms an inner surface of the neural conduit having a tube shape, the non-alignment portion forms an outer surface of the neural conduit having a tube shape, and the coupling alignment portion of the neural conduit having a tube shape. Neural conduit consisting of nanofibers, characterized in that arranged at both ends.
The method according to claim 17,

The pores formed in the non-aligned portion forming the outer surface of the neural conduit having a tube shape, the nerve conduit made of nanofibers, characterized in that the nutrients and oxygen permeate, and the size of the cells that make the wound block.
A mat forming step of forming a mat consisting of an alignment portion formed by aligning nanofibers and an unalignment portion formed by misaligning the nanofibers, integrally connected in the horizontal direction on the same plane by electrospinning;

And a tube forming step of winding the mat to form a tube shape in which the alignment portion is disposed on the inner side and the non-alignment portion is disposed on the outer side. 2.
The method of claim 24,

In the tube forming step, the method of manufacturing a neural conduit made of nanofibers, characterized in that the winding of the mat so that the nanofibers constituting the alignment portion in the longitudinal direction of the tube-shaped neural conduit is formed long.