WO2010151714A3 - Molecular arrays - Google Patents

Molecular arrays Download PDF

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Publication number
WO2010151714A3
WO2010151714A3 PCT/US2010/039882 US2010039882W WO2010151714A3 WO 2010151714 A3 WO2010151714 A3 WO 2010151714A3 US 2010039882 W US2010039882 W US 2010039882W WO 2010151714 A3 WO2010151714 A3 WO 2010151714A3
Authority
WO
WIPO (PCT)
Prior art keywords
spacer
arrays
array
compositions
systems
Prior art date
Application number
PCT/US2010/039882
Other languages
French (fr)
Other versions
WO2010151714A2 (en
Inventor
Zhaohui Zhou
George A. Fry
Sam Lee Woo
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2010151714A2 publication Critical patent/WO2010151714A2/en
Publication of WO2010151714A3 publication Critical patent/WO2010151714A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

The disclosure relates generally to compositions, systems, and methods for use in preparing single molecule arrays, for example, arrays of single nucleic acid molecules, as well as arrays formed using such compositions, systems and methods by using a spacer which restricts the spacing between adjacent components of the array. In an exemplary embodiment, the array is formed by contacting a surface with a spacer-anchor complex including one or more spacers linked to an anchor, wherein the spacer comprises a linear or branched polymeric molecule folded to form a three-dimensional shape of desired size dimensions. The size of the folded spacer may optionally be adjusted to modulate the spacing distance between adjacent array components.
PCT/US2010/039882 2009-06-24 2010-06-24 Molecular arrays WO2010151714A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22017409P 2009-06-24 2009-06-24
US61/220,174 2009-06-24

Publications (2)

Publication Number Publication Date
WO2010151714A2 WO2010151714A2 (en) 2010-12-29
WO2010151714A3 true WO2010151714A3 (en) 2011-05-26

Family

ID=43387133

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/039882 WO2010151714A2 (en) 2009-06-24 2010-06-24 Molecular arrays

Country Status (1)

Country Link
WO (1) WO2010151714A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8999674B2 (en) 2009-03-27 2015-04-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040142340A1 (en) * 2001-03-08 2004-07-22 Peter Estibeiro Detecting binding of mrna to an oligonucleotide array using rna dependent nucleic acid modifying enzymes
US20040146876A1 (en) * 2001-03-08 2004-07-29 Peter Estibeiro Complex element micro-array and methods of use
US20050100905A1 (en) * 2002-02-13 2005-05-12 Centre National De La Recherche Scientifique (Cnrs Novel method for production of dna biochips and applications thereof
US7270954B1 (en) * 2000-06-30 2007-09-18 Iris Biotechnologies, Inc. Hybridization of target DNA with immobilized nucleic acid analogs

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7270954B1 (en) * 2000-06-30 2007-09-18 Iris Biotechnologies, Inc. Hybridization of target DNA with immobilized nucleic acid analogs
US20040142340A1 (en) * 2001-03-08 2004-07-22 Peter Estibeiro Detecting binding of mrna to an oligonucleotide array using rna dependent nucleic acid modifying enzymes
US20040146876A1 (en) * 2001-03-08 2004-07-29 Peter Estibeiro Complex element micro-array and methods of use
US20050100905A1 (en) * 2002-02-13 2005-05-12 Centre National De La Recherche Scientifique (Cnrs Novel method for production of dna biochips and applications thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TOKUHISA, H. ET AL.: "Efficient biosensor interfaces based on space-controlled self-assembled monolayers", LANGMUIR, vol. 25, no. 3, 3 February 2009 (2009-02-03), pages 1633 - 1637 *
ZHAO, X. ET AL.: "Immobilization of oligodeoxyribonucleotides with multiple a nchors to microchips", NUCLEIC ACIDS RESEARCH, vol. 29, no. 4, 15 February 2001 (2001-02-15), pages 955 - 959 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8999674B2 (en) 2009-03-27 2015-04-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection

Also Published As

Publication number Publication date
WO2010151714A2 (en) 2010-12-29

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