WO2010022391A3 - Integrated, automated system for the study of cell and tissue function - Google Patents

Integrated, automated system for the study of cell and tissue function Download PDF

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Publication number
WO2010022391A3
WO2010022391A3 PCT/US2009/054781 US2009054781W WO2010022391A3 WO 2010022391 A3 WO2010022391 A3 WO 2010022391A3 US 2009054781 W US2009054781 W US 2009054781W WO 2010022391 A3 WO2010022391 A3 WO 2010022391A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
parameters
throughput
provides
cellular
Prior art date
Application number
PCT/US2009/054781
Other languages
French (fr)
Other versions
WO2010022391A9 (en
WO2010022391A2 (en
Inventor
Mark Holl
Deirdre Meldrum
Yassir Anis
Shashanka Ashili
Jeff Houkal
Roger Johnson
Laimonas Kelbauskas
Yongzhong Li
Saeed Merza
Vivek Nandakumar
Dean Smith
Cody Young
Xanqing Tian
Haixin Zhu
Joseph Chao
Original Assignee
Azte Arizona Technology Enterprises
The University Of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Azte Arizona Technology Enterprises, The University Of Washington filed Critical Azte Arizona Technology Enterprises
Publication of WO2010022391A2 publication Critical patent/WO2010022391A2/en
Publication of WO2010022391A9 publication Critical patent/WO2010022391A9/en
Publication of WO2010022391A3 publication Critical patent/WO2010022391A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/0092—Scheduling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/023—Sending and receiving of information, e.g. using bluetooth
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides for an automated, high-throughput, multi-parameter integrated analysis system to monitor, in parallel, cellular parameters descriptive of cell function and associated physiology both singly and in interactions with other cells in tissues. Parameters may be measured with cell or tissue samples confined in arrays of chambers microfabricated in cassettes. System variants provide for cell or tissue harvesting for (optionally destructive) further analysis. The present invention relates to a system for biological laboratory automation, providing for full automation of cell manipulation using capillary micropipettes and associated robotics. This invention provides a robust and flexible technology that can be deployed in research and corporate laboratories, enabling new experimental protocols. Applications include microinjection and high-throughput, single- to multi-cell, multi-parameter monitoring of parameters such as respiration rates, moiety of interest concentrations, metabolic fluxes, chemotactic gradients, gene expression and transcription, translation to proteins (intracellular, membrane, and extracellular), membrane integrity, and cellular ion gradients.
PCT/US2009/054781 2008-08-22 2009-08-24 Integrated, automated system for the study of cell and tissue function WO2010022391A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9103608P 2008-08-22 2008-08-22
US61/091,036 2008-08-22

Publications (3)

Publication Number Publication Date
WO2010022391A2 WO2010022391A2 (en) 2010-02-25
WO2010022391A9 WO2010022391A9 (en) 2010-04-15
WO2010022391A3 true WO2010022391A3 (en) 2010-06-17

Family

ID=41707688

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/054781 WO2010022391A2 (en) 2008-08-22 2009-08-24 Integrated, automated system for the study of cell and tissue function

Country Status (1)

Country Link
WO (1) WO2010022391A2 (en)

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US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
WO2008091626A1 (en) 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
GB201004614D0 (en) 2010-03-19 2010-05-05 Ge Healthcare Uk Ltd A system and method for automated extraction of multi-cellular physiological parameters
CN103249829B (en) * 2010-08-20 2015-08-19 孙钰 For the system and method for automatization sperm operation
DE102010037923B4 (en) * 2010-10-01 2015-08-27 Elmar Schmälzlin Apparatus and method for oxygen measurement
WO2012100764A1 (en) * 2010-11-17 2012-08-02 Als Automated Lab Solutions Gmbh Method and apparatus for automated preparation of cells for molecular biological investigations
WO2014093838A2 (en) 2012-12-14 2014-06-19 The J. David Gladstone Institutes Automated robotic microscopy systems
US10641772B2 (en) * 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
WO2017004250A1 (en) 2015-06-29 2017-01-05 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
US10695000B2 (en) 2015-09-02 2020-06-30 Metronom Health, Inc. Systems and methods for continuous health monitoring using an opto-enzymatic analyte sensor
EP3349900A4 (en) * 2015-09-18 2019-03-13 Arizona Board of Regents on behalf of Arizona State University Layered structure for improved sealing of microwell arrays
US11327004B2 (en) 2016-03-02 2022-05-10 Arizona Board Of Regents On Behalf Of Arizona State University Live-cell computed tomography
WO2017184998A1 (en) 2016-04-22 2017-10-26 Arizona Board Of Regents On Behalf Of Arizona State University A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation
GB2554334A (en) 2016-05-17 2018-04-04 Tap Biosystems Phc Ltd Automated bioprocess development
EP3487616B1 (en) 2016-07-21 2023-08-09 Takara Bio USA, Inc. Multi-z imaging of wells of multi-well devices and liquid dispensing into the wells
US11332782B2 (en) 2016-08-05 2022-05-17 Arizona Board Of Regents On Behalf Of Arizona State University Method for detecting nucleotide polymorphisms
JP2020500517A (en) 2016-11-28 2020-01-16 アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ Systems and methods relating to continuous flow droplet reactions
US10702690B2 (en) 2017-02-03 2020-07-07 Infinitesimal Llc Electroporation system with micromanipulator and probe
US11045807B2 (en) 2017-02-27 2021-06-29 Arizona Board Of Regents On Behalf Of Arizona State University Integrated platform for characterization of single cells or small cell clusters
US11315292B2 (en) 2017-03-02 2022-04-26 Arizona Board Of Regents On Behalf Of Arizona State University Live-cell computed tomography
US20200180991A1 (en) * 2017-04-28 2020-06-11 Corning Incorporated Glass structure, glass structure forming system, and method of making glass structure
US11618875B2 (en) 2017-11-20 2023-04-04 Nano Global Corporation Data collection and analytics based on detection of biological cells or biological substances
CN108239326B (en) * 2018-01-19 2020-09-08 深圳理科生科技有限公司 Intelligent respiration response material and preparation method thereof
JPWO2019163270A1 (en) * 2018-02-20 2021-01-07 ヤマハ発動機株式会社 Pick-up device for biological objects
WO2019226261A2 (en) * 2018-04-24 2019-11-28 Northwestern University Method and system for multispectral imaging
US11500528B2 (en) * 2019-07-01 2022-11-15 Palantir Technologies Inc. System architecture for cohorting sensor data
KR20220137756A (en) * 2020-02-10 2022-10-12 인테그리컬쳐 인코포레이티드 Cell Culture Management System
CN113528583A (en) * 2021-07-15 2021-10-22 澳门大学 Automatic microinjection method, device, system, equipment and storage medium
CN117555226B (en) * 2024-01-12 2024-03-12 北京智科特机器人科技有限公司 Laboratory automation oriented center management system and method

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US20020090320A1 (en) * 2000-10-13 2002-07-11 Irm Llc, A Delaware Limited Liability Company High throughput processing system and method of using
US20060164644A1 (en) * 2005-01-27 2006-07-27 Genetix Limited Robotic apparatus for picking of cells and other applications with integrated spectroscopic capability
US20070008528A1 (en) * 2005-07-07 2007-01-11 Chi-Hung Lin Device and method for simultaneous optical trapping, stretching, and real-time detection and measurement for morphological deformation of micro-particles
JP2008521425A (en) * 2004-12-03 2008-06-26 アイシー・イノベーションズ・リミテッド Methods for cell analysis

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US20020090320A1 (en) * 2000-10-13 2002-07-11 Irm Llc, A Delaware Limited Liability Company High throughput processing system and method of using
JP2008521425A (en) * 2004-12-03 2008-06-26 アイシー・イノベーションズ・リミテッド Methods for cell analysis
US20060164644A1 (en) * 2005-01-27 2006-07-27 Genetix Limited Robotic apparatus for picking of cells and other applications with integrated spectroscopic capability
US20070008528A1 (en) * 2005-07-07 2007-01-11 Chi-Hung Lin Device and method for simultaneous optical trapping, stretching, and real-time detection and measurement for morphological deformation of micro-particles

Also Published As

Publication number Publication date
WO2010022391A9 (en) 2010-04-15
WO2010022391A2 (en) 2010-02-25

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