WO2009140373A3 - Droplet actuator devices, systems, and methods - Google Patents

Droplet actuator devices, systems, and methods Download PDF

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Publication number
WO2009140373A3
WO2009140373A3 PCT/US2009/043774 US2009043774W WO2009140373A3 WO 2009140373 A3 WO2009140373 A3 WO 2009140373A3 US 2009043774 W US2009043774 W US 2009043774W WO 2009140373 A3 WO2009140373 A3 WO 2009140373A3
Authority
WO
WIPO (PCT)
Prior art keywords
droplet
droplet actuator
methods
systems
actuator devices
Prior art date
Application number
PCT/US2009/043774
Other languages
French (fr)
Other versions
WO2009140373A2 (en
Inventor
Michael Pollack
Zhishan Hua
Allen Eckhardt
Prasanna Thwar
Vijay Srinivasan
Vamsee Pamula
Original Assignee
Advanced Liquid Logic, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic, Inc. filed Critical Advanced Liquid Logic, Inc.
Priority to DK09747457.1T priority Critical patent/DK2279405T3/en
Priority to ES09747457.1T priority patent/ES2438989T3/en
Priority to US12/991,966 priority patent/US20110097763A1/en
Priority to EP09747457.1A priority patent/EP2279405B1/en
Priority to JP2011509649A priority patent/JP5592355B2/en
Priority to US12/546,115 priority patent/US8137917B2/en
Priority to US12/546,253 priority patent/US8088578B2/en
Publication of WO2009140373A2 publication Critical patent/WO2009140373A2/en
Publication of WO2009140373A3 publication Critical patent/WO2009140373A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

Abstract

The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
PCT/US2009/043774 2006-04-18 2009-05-13 Droplet actuator devices, systems, and methods WO2009140373A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DK09747457.1T DK2279405T3 (en) 2008-05-13 2009-05-13 Drip actuator devices, systems and methods
ES09747457.1T ES2438989T3 (en) 2008-05-13 2009-05-13 Devices, systems and droplet actuator methods
US12/991,966 US20110097763A1 (en) 2008-05-13 2009-05-13 Thermal Cycling Method
EP09747457.1A EP2279405B1 (en) 2008-05-13 2009-05-13 Droplet actuator devices, systems, and methods
JP2011509649A JP5592355B2 (en) 2008-05-13 2009-05-13 Droplet actuator device, system, and method
US12/546,115 US8137917B2 (en) 2006-04-18 2009-08-24 Droplet actuator devices, systems, and methods
US12/546,253 US8088578B2 (en) 2008-05-13 2009-08-24 Method of detecting an analyte

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US5288508P 2008-05-13 2008-05-13
US61/052,885 2008-05-13
US9886008P 2008-09-22 2008-09-22
US61/098,860 2008-09-22
US10333208P 2008-10-07 2008-10-07
US61/103,332 2008-10-07
US10888008P 2008-10-28 2008-10-28
US61/108,880 2008-10-28
US11565408P 2008-11-18 2008-11-18
US61/115,654 2008-11-18
US14182008P 2008-12-31 2008-12-31
US61/141,820 2008-12-31
US15359809P 2009-02-18 2009-02-18
US61/153,598 2009-02-18
US16060709P 2009-03-16 2009-03-16
US61/160,607 2009-03-16

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/639,490 Continuation-In-Part US7851184B2 (en) 2006-04-18 2006-12-15 Droplet-based nucleic acid amplification method and apparatus

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/546,253 Continuation US8088578B2 (en) 2008-05-13 2009-08-24 Method of detecting an analyte
US12/546,115 Continuation US8137917B2 (en) 2006-04-18 2009-08-24 Droplet actuator devices, systems, and methods

Publications (2)

Publication Number Publication Date
WO2009140373A2 WO2009140373A2 (en) 2009-11-19
WO2009140373A3 true WO2009140373A3 (en) 2010-04-22

Family

ID=41319311

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/043774 WO2009140373A2 (en) 2006-04-18 2009-05-13 Droplet actuator devices, systems, and methods

Country Status (6)

Country Link
US (2) US8137917B2 (en)
EP (3) EP2279405B1 (en)
JP (1) JP5592355B2 (en)
DK (1) DK2279405T3 (en)
ES (1) ES2438989T3 (en)
WO (1) WO2009140373A2 (en)

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US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use

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