WO2007092713A3 - Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease - Google Patents

Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease Download PDF

Info

Publication number
WO2007092713A3
WO2007092713A3 PCT/US2007/061352 US2007061352W WO2007092713A3 WO 2007092713 A3 WO2007092713 A3 WO 2007092713A3 US 2007061352 W US2007061352 W US 2007061352W WO 2007092713 A3 WO2007092713 A3 WO 2007092713A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
cassette
precancerous
cell
cancer
Prior art date
Application number
PCT/US2007/061352
Other languages
French (fr)
Other versions
WO2007092713A2 (en
Inventor
Barry L Ziober
Haim H Bau
Michael G Mauk
Zongyuan Chen
Jing Wang
Original Assignee
Univ Pennsylvania
Barry L Ziober
Haim H Bau
Michael G Mauk
Zongyuan Chen
Jing Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pennsylvania, Barry L Ziober, Haim H Bau, Michael G Mauk, Zongyuan Chen, Jing Wang filed Critical Univ Pennsylvania
Publication of WO2007092713A2 publication Critical patent/WO2007092713A2/en
Publication of WO2007092713A3 publication Critical patent/WO2007092713A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Abstract

Biomarkers for the detection of pre-cancer and cancer cells and a method for their detection at the point of care are disclosed. The system is comprised of an inexpensive, disposable microfluidic cassette for sample preparation; continuous-flow, magnetic-bead assisted cell sorting and cytometry; cell lysis; nucleic acid isolation and amplification, hybridization, quantification, and analysis. Typically, the disposable cassette will be used with a benchtop instrument platform that provides power, control and detection capabilities to the cassette. In one particular embodiment and application of the invention, the cell sorting is based on the selective binding of magnetic beads coated with an MCAP antibody to exfoliated precancerous and cancerous cells contained in saliva. The bead-labeled precancerous and cancerous cells are then separated from other saliva components using a magnetic field. Both labeled and unlabeled cells can be counted using integrated detectors of several types, including cell counters based on impedance measurements, and cytometers based on Coulter particle counter principles. The sorted sub-population of precancerous and cancerous cells are lysed, and the nucleic acids are isolated for gene assays and/or transcription profiling of the sorted sample subpopulation. In one embodiment of the invention, the cassette includes re verse- transcription polymerase chain reaction for nucleic acid detection and a hybridization array for gene identification. The devices, systems, modes of operation, and methods disclosed here are also applicable for cancer and other disease detection in other body fluids.
PCT/US2007/061352 2006-02-02 2007-01-31 Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease WO2007092713A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76439006P 2006-02-02 2006-02-02
US60/764,390 2006-02-02

Publications (2)

Publication Number Publication Date
WO2007092713A2 WO2007092713A2 (en) 2007-08-16
WO2007092713A3 true WO2007092713A3 (en) 2008-12-18

Family

ID=38345869

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/061352 WO2007092713A2 (en) 2006-02-02 2007-01-31 Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease

Country Status (1)

Country Link
WO (1) WO2007092713A2 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use
CN108485912A (en) * 2018-03-27 2018-09-04 上海默礼生物医药科技有限公司 A kind of miniature more warehouses control nucleic acid detection apparatus

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004047953A1 (en) * 2004-10-01 2006-04-20 Rudolf Rigler Selection of particle possessing predetermined property from population encompassing multiplicity of different particles, comprises providing population of different particles, and labeling particles which possess predetermined property
US20060264783A1 (en) 2005-05-09 2006-11-23 Holmes Elizabeth A Systems and methods for monitoring pharmacological parameters
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
EP2029779A4 (en) 2006-06-14 2010-01-20 Living Microsystems Inc Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US7807454B2 (en) 2006-10-18 2010-10-05 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for using the same
US20100151465A1 (en) 2008-03-27 2010-06-17 Jingyue Ju Selective Capture and Release of Analytes
AU2008308686B2 (en) 2007-10-02 2015-01-22 Labrador Diagnostics Llc Modular point-of-care devices and uses thereof
DE102007062441A1 (en) * 2007-12-20 2009-06-25 Aj Innuscreen Gmbh Mobile rapid test system for nucleic acid analysis
WO2009082242A2 (en) * 2007-12-21 2009-07-02 University Of Otago Optical analyte detection incorporating a micromirror device
DE102008009920A1 (en) * 2008-02-15 2009-08-20 Aj Innuscreen Gmbh Mobile device for nucleic acid isolation
US9090663B2 (en) 2009-04-21 2015-07-28 The Trustees Of Columbia University In The City Of New York Systems and methods for the capture and separation of microparticles
NZ595982A (en) * 2009-04-22 2013-10-25 Clinical Genomics Pty Ltd Method and apparatus for isolating a target bioentity from a biological sample
DE102009043426B4 (en) * 2009-09-29 2012-05-24 Siemens Aktiengesellschaft Apparatus and method for obtaining, detecting and analyzing cells in a microfluidic system
DE102009043537B4 (en) * 2009-09-30 2012-03-22 Siemens Aktiengesellschaft Method and device for determining cell vitalities
GB2474306A (en) * 2009-10-12 2011-04-13 Bioproducts Ltd Methods and device for detecting an analyte
BR112012009196B1 (en) 2009-10-19 2021-03-30 Labrador Diagnostics Llc SYSTEM FOR MODELING THE PROGRESSION OF A DISEASE WITHIN A POPULATION
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
EE201000013A (en) * 2010-01-29 2011-10-17 Selfdiagnostics O� Method and rapid assay device for detecting target rk molecule from sample material
AR085087A1 (en) 2011-01-21 2013-09-11 Theranos Inc SYSTEMS AND METHODS TO MAXIMIZE THE USE OF SAMPLES
AU2012220825B2 (en) * 2011-02-21 2015-12-17 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
EP2773775A4 (en) * 2011-11-04 2015-05-27 Diagnostics For All Inc Low cost, disposable molecular diagnostic devices
CN108587867B (en) 2012-05-25 2021-12-17 北卡罗来纳-查佩尔山大学 Microfluidic devices, solid supports for reagents, and related methods
EP2864781B1 (en) * 2012-06-22 2017-12-13 Scandinavian Micro Biodevices ApS A method and a system for quantitative or qualitative determination of a target component
EP2796876A1 (en) * 2013-04-25 2014-10-29 Hasenkam ApS Strip for monitoring analyte concentrations
CN103308692A (en) * 2013-05-22 2013-09-18 苏州市马尔泰新材料有限公司 CRISP1 (Cysteine-Rich Secretory Protein 1) gold-labeled kit for rapid oral floor carcinoma diagnosis
CN103267864A (en) * 2013-05-22 2013-08-28 苏州市马尔泰新材料有限公司 Application of kit for diagnosing oropharyngeal cancer
CN103308694A (en) * 2013-05-22 2013-09-18 苏州市马尔泰新材料有限公司 Gold-labeled kit for salivary gland carcinoma diagnosis
CN103278646B (en) * 2013-05-22 2015-01-28 上海市第十人民医院 Application of gold-labelled cysteine-rich secretory protein (CRISP)1 kit for diagnosing mouth floor carcinoma
CN103257241B (en) * 2013-05-22 2015-04-29 上海市第十人民医院 New application of CRISP (cysteine-rich secretory protein)1 to medical instrument
CN103308695A (en) * 2013-05-22 2013-09-18 苏州市马尔泰新材料有限公司 Cysteine-rich secretory protein 1 (CRISP1) kit for cheek carcinoma diagnosis
CN103267858B (en) * 2013-05-22 2015-03-25 上海市第十人民医院 Application of CRISP1 (Cysteine-Rich Secretory Protein 1) in preparing gold label test paper for diagnosing salivary gland carcinoma
CN103308691B (en) * 2013-05-22 2015-04-29 上海市第十人民医院 Application of secretory protein 1 rich in cysteine in preparation of product for diagnosing cheilocarcinoma
CN103257228A (en) * 2013-05-22 2013-08-21 苏州市马尔泰新材料有限公司 Novel application of CRISP1 (cysteine-rich secretory protein 1) kit for diagnosis on carcinoma of palate
CN103257239A (en) * 2013-05-22 2013-08-21 苏州市马尔泰新材料有限公司 Application of kit for quickly diagnosing carcinoma of salivary gland
CN103267848A (en) * 2013-05-22 2013-08-28 苏州市马尔泰新材料有限公司 CRISP1 (cystein-rich secretory protein 1) kit for quickly diagnosing gingival carcinoma
CN103267859B (en) * 2013-05-22 2015-03-11 上海市第十人民医院 Application of CRISP1 (cystein-rich secretory protein 1) test paper for diagnosing gingival carcinoma
CN103293320B (en) * 2013-05-22 2015-03-25 上海市第十人民医院 Application of cysteine-rich secretory protein 1 (CRISP 1) in preparation of gingival cancer diagnosis products
CN103267856B (en) * 2013-05-22 2015-04-29 上海市第十人民医院 Application of kit for diagnosing jaw cancer
CN103257242B (en) * 2013-05-22 2015-01-28 上海市第十人民医院 Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of buccal mucosa
CN103293319A (en) * 2013-05-22 2013-09-11 苏州市马尔泰新材料有限公司 Gold-labeled test paper for rapidly diagnosing carcinoma jaw
CN103267857A (en) * 2013-05-22 2013-08-28 苏州市马尔泰新材料有限公司 CRISP1 (cystein-rich secretory protein 1) test paper for diagnosing tongue cancer
CN103308682A (en) * 2013-05-22 2013-09-18 苏州市马尔泰新材料有限公司 Kit for rapid lip cancer diagnosis
CN103293296A (en) * 2013-05-22 2013-09-11 苏州市马尔泰新材料有限公司 Application of cysteine-rich secretory protein 1 (CRISP 1) in preparation of kit for diagnosing carcinoma of floor of mouth
CN103267861B (en) * 2013-05-22 2015-01-14 上海市第十人民医院 Application of cysteine-rich secretory protein 1 in manufacturing product for diagnosing palate carcinoma
CN103308693A (en) * 2013-05-22 2013-09-18 苏州市马尔泰新材料有限公司 Application of secretory protein 1 rich in cysteine in medical apparatus
CN103257240B (en) * 2013-05-22 2015-03-25 上海市第十人民医院 Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue
CN103278644A (en) * 2013-05-22 2013-09-04 苏州市马尔泰新材料有限公司 Gold-labelled CRISP (cysteine-rich secretory protein)1 kit for quickly diagnosing carcinoma of palate
CN103278629A (en) * 2013-05-22 2013-09-04 苏州市马尔泰新材料有限公司 Gold-labelled kit for quickly diagnosing oropharynx carcinoma
CN103267863A (en) * 2013-05-22 2013-08-28 苏州市马尔泰新材料有限公司 CRISP1 (cystein-rich secretory protein 1) test paper for quickly diagnosing carcinoma of maxillary sinus
CN103278645B (en) * 2013-05-22 2015-01-28 上海市第十人民医院 Application of gold-labelled test strip for quickly diagnosing carcinoma of lip
CN103267860B (en) * 2013-05-22 2015-04-29 上海市第十人民医院 Application of CRISP1 (Cysteine-Rich Secretory Protein 1) in preparing kit for diagnosing jawbone carcinoma
CN103267862A (en) * 2013-05-22 2013-08-28 苏州市马尔泰新材料有限公司 Application of CRISP1 (Cysteine-Rich Secretory Protein 1) in preparing gold label kit for diagnosing maxillary sinus carcinoma
CN103257238B (en) * 2013-05-22 2015-01-28 上海市第十人民医院 Application of CRISP (cysteine-rich secretory protein)1 test paper for diagnosing carcinoma of maxillary sinus
CN103411981B (en) * 2013-07-25 2016-06-22 宁波大学 One utilizes dielectric and magnetic characteristic to differentiate the epithelioglandular method of gastric cancer
FR3009082B1 (en) * 2013-07-26 2016-10-21 Centre Nat Rech Scient MICROFLUIDIC SYSTEM AND METHOD FOR ISOLATING AND QUANTIFYING AT LEAST ONE SUB-POPULATION OF CELLS FROM A POPULATION OF CELLS
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
KR101702745B1 (en) * 2014-11-13 2017-02-03 인제대학교 산학협력단 Apparatus and method for separating single cell
US10864520B2 (en) * 2015-07-22 2020-12-15 The University Of North Carolina At Chapel Hill Fluidic devices with freeze-thaw valves with ice-nucleating agents and related methods of operation and analysis
WO2017065854A2 (en) 2015-07-22 2017-04-20 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
EP4102226A1 (en) 2015-12-08 2022-12-14 Berkeley Lights, Inc. In situ-generated microfluidic assay structures, related kits, and methods of use thereof
EP3402814B1 (en) * 2016-01-15 2021-04-21 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
CN109416311A (en) 2016-07-26 2019-03-01 惠普发展公司，有限责任合伙企业 Microfluidic device for the mobile control of fluid
IL266208B1 (en) * 2016-10-23 2024-01-01 Berkeley Lights Inc Methods for Screening B Cell Lymphocytes
EP3609619A1 (en) * 2017-04-14 2020-02-19 University Of Twente Device and method for the continuous trapping of circulating tumor cells
JP2021515220A (en) * 2018-03-01 2021-06-17 アカデミア シニカＡｃａｄｅｍｉａ Ｓｉｎｉｃａ Devices and methods for detecting cancer cells
KR102401909B1 (en) * 2018-08-30 2022-05-24 주식회사 엘지화학 A high-speed screening analysis system for reaction optimization
WO2020068069A1 (en) * 2018-09-26 2020-04-02 Hewlett-Packard Development Company, L.P. Dielectrophoresis and density separators
WO2021234729A1 (en) * 2020-05-16 2021-11-25 Indian Institute Of Science Education And Research (Iiser) Kolkata Laser induced micro-bubble based patterning of biological macromolecules towards bio-sensing applications
CN111944656B (en) * 2020-07-06 2022-07-29 东南大学 Microfluidic cell magnetic capture and detection system and capture and detection method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013645A (en) * 1987-04-14 1991-05-07 Abbott Laboratories Immunological methods and materials for detection of tumor associated antigens
US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US6103537A (en) * 1997-10-02 2000-08-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6268219B1 (en) * 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device
US6355491B1 (en) * 1999-03-15 2002-03-12 Aviva Biosciences Individually addressable micro-electromagnetic unit array chips

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013645A (en) * 1987-04-14 1991-05-07 Abbott Laboratories Immunological methods and materials for detection of tumor associated antigens
US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US6103537A (en) * 1997-10-02 2000-08-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6355491B1 (en) * 1999-03-15 2002-03-12 Aviva Biosciences Individually addressable micro-electromagnetic unit array chips
US6268219B1 (en) * 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use
CN108485912A (en) * 2018-03-27 2018-09-04 上海默礼生物医药科技有限公司 A kind of miniature more warehouses control nucleic acid detection apparatus
CN108485912B (en) * 2018-03-27 2021-05-07 上海默礼生物医药科技有限公司 Miniature many storehouses control nucleic acid detection device

Also Published As

Publication number Publication date
WO2007092713A2 (en) 2007-08-16

Similar Documents

Publication Publication Date Title
WO2007092713A3 (en) Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
Wu et al. Single-cell protein analysis
Zhang et al. Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level
Clayton et al. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017
KR102243597B1 (en) Selective delivery of material to cells
Gascoyne et al. Microfluidic approaches to malaria detection
Ying et al. Microfluidic chip-based technologies: emerging platforms for cancer diagnosis
WO2009051734A1 (en) Microchip-based devices for capturing circulating tumor cells and methods of their use
WO2021170009A1 (en) Fetal cell capture module and microfluidic chip for fetal cell capture and methods for using the same
AU2006292394A1 (en) Systems and methods for enrichment of analytes
WO2020043036A1 (en) Co-detection and digital quantification of bioassay
CN111500769A (en) Fluorescence immunochromatography method for detecting SARS-CoV-2 nucleic acid
Di Carlo et al. Introduction: why analyze single cells?
Suzuki et al. Mechanical low-pass filtering of cells for detection of circulating tumor cells in whole blood
Inci et al. Bio-inspired magnetic beads for isolation of sperm from heterogenous samples in forensic applications
Jiao et al. Recent advances in microfluidic single-cell analysis and its applications in drug development
Wang et al. Sickle-like inertial microfluidic system for online rare cell separation and tandem label-free quantitative proteomics (Orcs-Proteomics)
US20160258938A1 (en) Method of detecting an analyte in a sample
Xiao et al. A rapid, simple, and low-cost CD4 cell count sensor based on blocking immunochromatographic strip system
Schröder et al. Immuno-PCR with digital readout
EP3698136A1 (en) Disease proteome protein arrays and uses thereof
Zhang et al. Microfluidics for sepsis early diagnosis and prognosis: a review of recent methods
Watkins et al. On a chip
Feng et al. Microfluidics-based single cell sequencing: moving towards more physiological analysis
Pandya et al. Lab-on-a-chip-oral cancer diagnosis at your door step

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07763203

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 07763203

Country of ref document: EP

Kind code of ref document: A2