WO2007087339A3 - Multiplexed analyte quantitation by two-dimensional planar electrochromatography - Google Patents

Multiplexed analyte quantitation by two-dimensional planar electrochromatography Download PDF

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Publication number
WO2007087339A3
WO2007087339A3 PCT/US2007/001865 US2007001865W WO2007087339A3 WO 2007087339 A3 WO2007087339 A3 WO 2007087339A3 US 2007001865 W US2007001865 W US 2007001865W WO 2007087339 A3 WO2007087339 A3 WO 2007087339A3
Authority
WO
WIPO (PCT)
Prior art keywords
dimensional planar
planar electrochromatography
analyte quantitation
multiplexed analyte
analyte
Prior art date
Application number
PCT/US2007/001865
Other languages
French (fr)
Other versions
WO2007087339A2 (en
Inventor
Wayne F Patton
Linan Song
Nancy C Wilker
Original Assignee
Perkinelmer Las Inc
Wayne F Patton
Linan Song
Nancy C Wilker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkinelmer Las Inc, Wayne F Patton, Linan Song, Nancy C Wilker filed Critical Perkinelmer Las Inc
Publication of WO2007087339A2 publication Critical patent/WO2007087339A2/en
Publication of WO2007087339A3 publication Critical patent/WO2007087339A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Abstract

The invention relates to methods for isolating an analyte of interest in a sample suspected of containing the analyte of interest using two-dimensional planar electrochromatography. The methods comprise treating at least a portion of the sample with a mobility modifier capable of modifying the mobility of the analyte of interest after the second dimension of planar electrochromatography. Kits and compositions are also disclosed.
PCT/US2007/001865 2006-01-24 2007-01-24 Multiplexed analyte quantitation by two-dimensional planar electrochromatography WO2007087339A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76158406P 2006-01-24 2006-01-24
US60/761,584 2006-01-24

Publications (2)

Publication Number Publication Date
WO2007087339A2 WO2007087339A2 (en) 2007-08-02
WO2007087339A3 true WO2007087339A3 (en) 2008-01-24

Family

ID=38309814

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/001865 WO2007087339A2 (en) 2006-01-24 2007-01-24 Multiplexed analyte quantitation by two-dimensional planar electrochromatography

Country Status (2)

Country Link
US (1) US20070251824A1 (en)
WO (1) WO2007087339A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047863A (en) * 2016-06-18 2016-10-26 贵州师范学院 Method for labeling nucleic acid probes by aid of isotopes by means of gel cutting and purifying

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7550271B2 (en) * 2006-04-28 2009-06-23 Perkinelmer Las, Inc. Detecting phospho-transfer activity
US8278057B2 (en) 2007-09-14 2012-10-02 Nestec S.A. Addressable antibody arrays and methods of use
CA2754980A1 (en) * 2009-03-18 2010-09-23 Prometheus Laboratories Inc. Addressable antibody arrays and methods of use
US9470664B2 (en) * 2009-04-10 2016-10-18 Waters Technologies Corporation Chromatographic interface
JP5893607B2 (en) 2010-04-05 2016-03-23 プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Spatial-encoded biological assay
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
CA2924284C (en) * 2013-09-13 2022-01-04 The Board Of Trustees Of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN109164177B (en) * 2018-08-13 2021-03-09 中国石油化工股份有限公司 Method for measuring aldehyde content in ester product
CN111175388B (en) * 2019-11-18 2022-12-06 远大医药（中国）有限公司 Method for determining DCC content in indapamide bulk drug
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2023177763A2 (en) * 2022-03-15 2023-09-21 Buzzkill Labs, Inc. Method and apparatus for separating and concentrating analytes
CN115166127A (en) * 2022-07-26 2022-10-11 吉首大学 Thin layer identification method from complex two-dimensional to simple one-dimensional

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040137526A1 (en) * 2002-10-15 2004-07-15 The Regents Of The University Of Michigan Multidimensional protein separation system
US20050269267A1 (en) * 2004-03-19 2005-12-08 Perkinelmer Las, Inc. Separations platform based upon electroosmosis-driven planar chromatography
WO2007058893A2 (en) * 2005-11-10 2007-05-24 Perkinelmer Life And Analytical Sciences Planar electrochromatography/thin layer chromatography separations systems

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2555487A (en) * 1948-02-27 1951-06-05 United Shoe Machinery Corp Chromatographic process and apparatus
US3984298A (en) * 1970-12-28 1976-10-05 Haber Instruments, Incorporated Electromolecular propulsion in semiconductive media
JPS5379060A (en) * 1976-12-23 1978-07-13 Tokuyama Soda Kk Method of salt adjusting and removing for milk and other analogue
US4315812A (en) * 1980-05-28 1982-02-16 Karlson Eskil L Apparatus for continuous electrochromatographic separation
US4931498A (en) * 1988-02-25 1990-06-05 Purdue Research Foundation Immobilized artificial membranes
US5792936A (en) * 1990-06-23 1998-08-11 Hoechst Aktiengesellschaft Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation
US5160627A (en) * 1990-10-17 1992-11-03 Hoechst Celanese Corporation Process for making microporous membranes having gel-filled pores, and separations methods using such membranes
US20020082391A1 (en) * 1996-03-15 2002-06-27 Anderson James M. Human occludin, its uses and enhancement of drug absorption using occludin inhibitors
EP1019034A2 (en) * 1997-07-01 2000-07-19 Atherogenics, Inc. Antioxidant enhancement of therapy for hyperproliferative conditions
DE19937438C2 (en) * 1999-08-07 2001-09-13 Bruker Daltonik Gmbh Coupling thin layer chromatography and mass spectrometry (TLC / MS)
US6303029B1 (en) * 1999-10-25 2001-10-16 Advanced Research And Technology Arrangement and method for performing chromatography
CA2311622A1 (en) * 2000-06-15 2001-12-15 Moussa Hoummady Sub-nanoliter liquid drop dispensing system and method therefor
US6824981B2 (en) * 2000-08-11 2004-11-30 Agilix Corporation Ultra-sensitive detection systems using alterable peptide tags
US20030217389A1 (en) * 2001-02-15 2003-11-20 Ryan Clarence A Novel peptides and methods of use
US7572357B2 (en) * 2001-07-25 2009-08-11 The United States of America as represented by the Secretary of Commerce, the National Institute of Standards and Technology Chiral temperature gradient focusing
US7132295B2 (en) * 2001-11-09 2006-11-07 Bayer Aktiengesellschaft Isotopically coded affinity markers 3
SE0202399D0 (en) * 2001-12-11 2002-08-13 Thomas Laurell Device and method usable for integrated sequential separation and enrichment of proteins
JP4303595B2 (en) * 2001-12-21 2009-07-29 透 小池 Zinc complexes capable of trapping substances with anionic substituents
CA2498354C (en) * 2002-09-11 2012-03-20 Temple University - Of The Commonwealth System Of Higher Education System and methods for electrophoretic separation of proteins on protein binding membranes
TW200502238A (en) * 2003-03-03 2005-01-16 Nard Kenkyusho Kk Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
WO2004078342A1 (en) * 2003-03-04 2004-09-16 Manac Inc. Scavenger for substance having anionic substituent
TW200508606A (en) * 2003-03-07 2005-03-01 Nard Kenkyusho Kk Method of finding molecular weight of phosphoric acid monoester compound, and additive for mass spectrometric measurement
US20050009114A1 (en) * 2003-05-15 2005-01-13 Board Of Regents, The University Of Texas System Identification and quantification of organ-specific proteins derived from human allogeneic cells using proteomics
US20050164324A1 (en) * 2003-06-04 2005-07-28 Gygi Steven P. Systems, methods and kits for characterizing phosphoproteomes
US7736517B2 (en) * 2003-06-19 2010-06-15 Indiana University Research And Technology Corporation Method and apparatus for performing planar electrochromatography at elevated pressure
US20050148087A1 (en) * 2004-01-05 2005-07-07 Applera Corporation Isobarically labeled analytes and fragment ions derived therefrom
WO2007067731A2 (en) * 2005-12-08 2007-06-14 Perkinelmer Las, Inc. Micelle- and microemulsion-assisted planar separations platform for proteomics

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040137526A1 (en) * 2002-10-15 2004-07-15 The Regents Of The University Of Michigan Multidimensional protein separation system
US20050269267A1 (en) * 2004-03-19 2005-12-08 Perkinelmer Las, Inc. Separations platform based upon electroosmosis-driven planar chromatography
WO2007058893A2 (en) * 2005-11-10 2007-05-24 Perkinelmer Life And Analytical Sciences Planar electrochromatography/thin layer chromatography separations systems

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NUROK: "Planar Electrochromatography", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER B.V., vol. 1044, 2004, pages 83 - 96 *
PATTON ET AL.: "Taking a Walk on the Wild Side with Planar Electrochromatography and Thin-Layer Electrophoresis: Of Peptides, Proteins, and Proteomics", JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, vol. 29, 2006, pages 1177 - 1218 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047863A (en) * 2016-06-18 2016-10-26 贵州师范学院 Method for labeling nucleic acid probes by aid of isotopes by means of gel cutting and purifying

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Publication number Publication date
WO2007087339A2 (en) 2007-08-02
US20070251824A1 (en) 2007-11-01

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