WO2007020457A3 - Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use - Google Patents
Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use Download PDFInfo
- Publication number
- WO2007020457A3 WO2007020457A3 PCT/GB2006/003095 GB2006003095W WO2007020457A3 WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3 GB 2006003095 W GB2006003095 W GB 2006003095W WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3
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- WIPO (PCT)
- Prior art keywords
- nucleotides
- methods
- group
- polyethylene glycol
- base
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
Modified guanine-containing nucleosides and nucleotides, in particular fluorescently labelled guanine-containing nucleosides and nucleotides, which exhibit reduced quenching effects, and hence enhanced brightness of the fluorophore are described.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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EP06779167.3A EP1926829B2 (en) | 2005-08-19 | 2006-08-18 | Labelled modified guanine-containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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GB0517097.2 | 2005-08-19 | ||
GBGB0517097.2A GB0517097D0 (en) | 2005-08-19 | 2005-08-19 | Modified nucleosides and nucleotides and uses thereof |
Publications (2)
Publication Number | Publication Date |
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WO2007020457A2 WO2007020457A2 (en) | 2007-02-22 |
WO2007020457A3 true WO2007020457A3 (en) | 2007-10-25 |
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Family Applications (1)
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PCT/GB2006/003095 WO2007020457A2 (en) | 2005-08-19 | 2006-08-18 | Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use |
Country Status (4)
Country | Link |
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US (3) | US7592435B2 (en) |
EP (1) | EP1926829B2 (en) |
GB (1) | GB0517097D0 (en) |
WO (1) | WO2007020457A2 (en) |
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Also Published As
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EP1926829B2 (en) | 2017-07-19 |
US20070042407A1 (en) | 2007-02-22 |
EP1926829A2 (en) | 2008-06-04 |
US7592435B2 (en) | 2009-09-22 |
US20110020827A1 (en) | 2011-01-27 |
GB0517097D0 (en) | 2005-09-28 |
WO2007020457A2 (en) | 2007-02-22 |
US20100068722A1 (en) | 2010-03-18 |
US8212015B2 (en) | 2012-07-03 |
US7816503B2 (en) | 2010-10-19 |
EP1926829B1 (en) | 2014-08-06 |
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