WO2006022636A1 - Methods for identifying risk of type ii diabetes and treatments thereof - Google Patents

Methods for identifying risk of type ii diabetes and treatments thereof Download PDF

Info

Publication number
WO2006022636A1
WO2006022636A1 PCT/US2004/023933 US2004023933W WO2006022636A1 WO 2006022636 A1 WO2006022636 A1 WO 2006022636A1 US 2004023933 W US2004023933 W US 2004023933W WO 2006022636 A1 WO2006022636 A1 WO 2006022636A1
Authority
WO
WIPO (PCT)
Prior art keywords
diabetes
type
polymorphic
nucleic acid
subject
Prior art date
Application number
PCT/US2004/023933
Other languages
French (fr)
Inventor
Maria L. Langdown
Matthew Roberts Nelson
Rikard Henry Reneland
Stefan M. Kammerer
Andreas Braun
Carolyn R. Hoyal-Wrightson
Original Assignee
Sequenom, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequenom, Inc. filed Critical Sequenom, Inc.
Priority to PCT/US2004/023933 priority Critical patent/WO2006022636A1/en
Publication of WO2006022636A1 publication Critical patent/WO2006022636A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Definitions

  • the invention relates to genetic methods for identifying predisposition to type II diabetes, also known as non-insulin dependent diabetes, and treatments that specifically target the disease.
  • Type I diabetes insulin-dependent diabetes
  • pancreatic ⁇ -cells pancreatic ⁇ -cells with subsequent insulin deficiency.
  • Type II diabetes non-insulin dependent diabetes
  • Type II diabetes represents 90-95% of the affected population, more than 100 million people worldwide. Approximately 17 million Americans suffer from type II diabetes, although 6 million don't even know they have the disease. The prevalence of the disease has jumped 33% in the last decade and is expected to rise further as the baby boomer generation gets older and more overweight. The global figure of people with diabetes is set to rise to an estimated 150 to 220 million in 2010, and 300 million in 2025. The widespread problem of diabetes has crept up on an unsuspecting health care community and has already imposed a huge burden on health-care systems (Zimmet et al (2001) Nature 414: 782-787).
  • the onsebof type II diabetes can be insidious, or even clinically unapparent, making diagnosis difficult. Even when the disease is properly diagnosed, many of those treated do not have adequate control over their diabetes, resulting in elevated sugar levels in the bloodstream that slowly destroys the kidneys, eyes, blood vessels and nerves. This late damage is an important factor contributing to mortality in diabetics.
  • Type II diabetes is associated with peripheral insulin resistance, elevated hepatic glucose production, and inappropriate insulin secretion (DeFronzo, R. A. (1988) Diabetes 37:667- 687), although the primary pathogenic lesion on type II diabetes remains elusive. Many have suggested that primary insulin resistance of the peripheral tissues is the initial event. Genetic epidemiological studies have supported this view. Similarly, insulin secretion abnormalities have been argued as the primary defect in type II diabetes. It is likely that both phenomena are important in the development of type II diabetes, and genetic defects predisposing to both are likely to be important contributors to the disease process (Rimoin, D.L., et al. (1996) Emery and Rimoin's Principles and Practice of Medical Genetics 3rd Ed. 1: 1401-1402).
  • polymorphic variations in human genomic DNA are associated with the occurrence of type II diabetes, also known as non-insulin dependent diabetes.
  • polymorphic variants in loci containing SLC22A1, TTN, LOCI 12609 (also known as C6orfll7), TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLLS regions in human genomic DNA have been associated with risk of type II diabetes.
  • identifying a subject at risk of type II diabetes and/or a risk of type II diabetes in a subject which comprise detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in or around loci described herein in a human nucleic acid sample.
  • two or more polymorphic variations are detected and in some embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variants are detected.
  • nucleic acids that include one or more polymorphic variations associated with occurrence of type II diabetes, as well as polypeptides encoded by these nucleic acids.
  • methods for identifying candidate therapeutic molecules for treating type II diabetes and other insulin-related disorders as well as methods for treating type II diabetes in a subject by identifying a subject at risk of type II diabetes and treating the subject with a suitable prophylactic, treatment or therapeutic molecule.
  • compositions comprising a cell from a subject having type II diabetes or at risk of type II diabetes and/or a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid, with a RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid designed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a nucleic acid that hybridizes to such a nucleotide sequence under stringent conditions.
  • the RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid is designed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence that includes one or more type II diabetes associated polymorphic variations, and in some instances, specifically interacts with such a nucleotide sequence.
  • nucleic acids bound to a solid surface in which one or more nucleic acid molecules of the array have a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a fragment or substantially identical nucleic acid thereof, or a complementary nucleic acid of the foregoing.
  • compositions comprising a cell from a subject having type II diabetes or at risk of type II diabetes and/or a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB polypeptide, with an antibody that specifically binds to the polypeptide.
  • the antibody specifically binds to an epitope in the polypeptide that includes a non-synonymous amino acid modification associated with type II diabetes (e.g., results in an amino acid substitution in the encoded polypeptide associated with type II diabetes).
  • the antibody specifically binds to an epitope comprising a glycine corresponding to position 557 in a ZNF221 polypeptide (e.g., a polypeptide having an amino acid sequence in SEQ ID NO: 27).
  • Figures 1 A-IC show proximal SNPs in a SLC22A1 region in genomic DNA for females, males and combined results, respectively.
  • Figures 2A-2C show proximal SNPs in a TTN region in genomic DNA for females, males and combined results, respectively.
  • Figures 3A-3C show proximal SNPs in a LOCI 12609 region in genomic DNA for females, males and combined results, respectively.
  • Figures 4A-4C show proximal SNPs in a TRPSl region in genomic DNA for females, males and combined results, respectively.
  • Figures 5A-5C show proximal SNPs in a PRAME region in genomic DNA for females, males and combined results, respectively.
  • Figures 6A-6C show proximal SNPs in a ZNF221 region in genomic DNA for females, males and combined results, respectively.
  • Figures 7A-7C show proximal SNPs in a FOXA2 region in genomic DNA for females, males and combined results, respectively.
  • Figures 8A-8C show proximal SNPs in a JAMS region in genomic DNA for females, males and combined results, respectively.
  • Figures 9A-9C show proximal SNPs in a GLB region in genomic DNA for females, males and combined results, respectively.
  • each SNP in the chromosome is shown on the x-axis and the y-axis provides the negative logarithm of the p-value comparing the estimated allele frequency in the cases to that of the control group. Also shown in the figures are exons and introns of the genes in approximate chromosomal positions.
  • Figures 1 OA-I OG show results of an odds-ratio meta analysis for SLC22A1, LOCI 12609, PRAME, ZNF221, FOXA2, JAM3 and GLB regions, respectively.
  • polymorphic variants described in a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB locus in human genomic DNA are associated with occurrence of type II diabetes in subjects.
  • detecting genetic determinants in and around this locus associated with an increased risk of type II diabetes occurrence can lead to early identification of a risk of type II diabetes and early application of preventative and treatment measures.
  • Associating the polymorphic variants with type II diabetes also has provided new targets for diagnosing type II diabetes, and methods for screening molecules useful in diabetes treatments and diabetes preventatives.
  • SLC22A1 encodes solute carrier family 22, member 1, which is a polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs from the systemic circulation, maintaining body fluid homeostasis, and acting as a defense system against toxic agents.
  • This gene is one of three similar cation transporter genes located in a cluster on chromosome 6.
  • the encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein.
  • TTN encodes a 3-megadalton protein composed of 244 copies of immunoglobulin and fibronectin type III domains.
  • TITIN is stringlike in structure and in vivo spans from the M to Z lines, important for structural integrity of the myofibril and passive tension.
  • Titin phosphorylates Titin-cap (a 19 kDa protein) a protein that associates with the N-terminus of titin.
  • TRPSl trichorhinophalangeal syndrome I
  • FOXA2 encodes a polypeptide that is a member of a forkhead class of DNA-binding proteins referred to as forkhead box A2.
  • FOXA2 consists of two transcript variants, which differ in the 5' UTR but encode the same protein.
  • FOXA2 serves as a transcription factor involved in the regulation of pancreatic development. Its expression is known to be regulated by HNF6 in human hepatoma cells.
  • the JAM3 gene encodes a protein that is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.
  • the protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. Tight junctions represent one mode of cell- to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.
  • JAM3 gene contains 9 exons and spans more than 88 kb.
  • the deduced 310- arnino acid protein is more than 30% identical to JAM2 and JAMl . It possesses a signal sequence; 2 Ig-like folds, one a V type and the other a C2 type, containing 6 cysteines; 2 potential N- glycosylation sites; and a 46-amino acid intracellular tail with a C-terminal binding motif for PDZ domains and a phosphorylation site.
  • Northern blot analysis revealed wide expression of an approximately 3.3-kb transcript, with highest levels in placenta, brain, and kidney. Expression was also detected in cultured endothelial cells.
  • the GLB gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the GIi family. They are characterized as a transcription factor that binds to DNA through zinc finger motifs. These motifs have conserved H-C links. GIi family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial Polydactyly type IV, postaxial Polydactyly types Al and B.
  • Type II diabetes refers to non-insulin-dependent diabetes.
  • Type II diabetes refers to an insulin-related disorder in which there is a relative disparity between endogenous insulin production and insulin requirements, leading to elevated hepatic glucose production, elevated blood glucose levels, inappropriate insulin secretion, and peripheral insulin resistance.
  • Type II diabetes has been regarded as a relatively distinct disease entity, but type II diabetes is often a manifestation of a much broader underlying disorder (Zimmet et al (2001) Nature 414: 782-787), which may include metabolic syndrome (syndrome X), diabetes (e.g., type I diabetes, type II diabetes, gestational diabetes, autoimmune diabetes), hyperinsulinemia, hyperglycemia, impaired glucose tolerance (IGT), hypoglycemia, B-cell failure, insulin resistance, dyslipidemias, atheroma, insulinoma, hypertension, hypercoagulability, microalbuminuria, and obesity and obesity-related disorders such as visceral obesity, central fat, obesity-related type II diabetes, obesity-related atherosclerosis, heart disease, obesity-related insulin resistance, obesity- related hypertension, microangiopathic lesions resulting from obesity-related type II diabetes, ocular lesions caused by microangiopathy in obese individuals with obesity-related type II diabetes, and renal lesions caused by microangiopathy in obese individuals with obesity-related type II
  • type II diabetes Some of the more common adult onset diabetes symptoms include fatigue, excessive thirst, frequent urination, blurred vision, a high rate of infections, wounds that heal slowly, mood changes and sexual problems. Despite these known symptoms, the onset of type II diabetes is often not discovered by health care professionals until the disease is well developed. Once identified, type II diabetes can be recognized in a patient by measuring fasting plasma glucose levels and/or casual plasma glucose levels, measuring fasting plasma insulin levels and/or casual plasma insulin levels, or administering oral glucose tolerance tests or hyperinsulinemic euglycemic clamp tests.
  • individuals having type II diabetes can be selected for genetic studies. Also, individuals having no history of metabolic disorders, particularly type II diabetes, often are selected for genetic studies as controls. The individuals selected for each pool of case and controls, were chosen following strict selection criteria in order to make the pools as homogenous as possible. Selection criteria for the study described herein included patient age, ethnicity, BMI, GAD (Glutamic Acid Decarboxylase) antibody concentration, and HbAIc (glycosylated hemoglobin AIc) concentration.
  • GAD antibody is present in association ⁇ with islet cell destruction, and therefore can be utilized to differentiate insulin dependent diabetes (type I diabetes) from non-insulin dependent diabetes (type II diabetes). HbAIc levels will reveal the average blood glucose over a period of 2-3 months or more specifically, over the life span of a red blood cell, by recording the number of glucose molecules attached to hemoglobin.
  • polymorphic site refers to a region in a nucleic acid at which two or more alternative nucleotide sequences are observed in a significant number of nucleic acid samples from a population of individuals.
  • a polymorphic site may be a nucleotide sequence of two or more nucleotides, an inserted nucleotide or nucleotide sequence, a deleted nucleotide or nucleotide sequence, or a microsatellite, for example.
  • a polymorphic site that is two or more nucleotides in length may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, 20 or more, 30 or more, 50 or more, 75 or more, 100 or more, 500 or more, or about 1000 nucleotides in length, where all or some of the nucleotide sequences differ within the region.
  • a polymorphic site is often one nucleotide in length, which is referred to herein as a "single nucleotide polymorphism" or a "SNP.”
  • each nucleotide sequence is referred to as a "polymorphic variant" or "nucleic acid variant.”
  • polymorphic variants represented in a minority of samples from a population is sometimes referred to as a “minor allele” and the polymorphic variant that is more prevalently represented is sometimes referred to as a "major allele.”
  • minor allele the polymorphic variant represented in a minority of samples from a population
  • major allele the polymorphic variant that is more prevalently represented
  • Individuals who are homozygous with respect to one allele are sometimes predisposed to a different phenotype as
  • allelotyped and/or genotyped refers to a process for determining the allele frequency for a polymorphic variant in pooled DNA samples from cases and controls. By pooling DNA from each group, an allele frequency for each SNP in each group is calculated. These allele frequencies are then compared to one another.
  • genotyped refers to a process for determining a genotype of one or more individuals, where a “genotype” is a representation of one or more polymorphic variants in a population.
  • a genotype or polymorphic variant may be expressed in terms of a "haplotype," which as used herein refers to two or more polymorphic variants occurring within genomic DNA in a group of individuals within a population.
  • haplotype refers to two or more polymorphic variants occurring within genomic DNA in a group of individuals within a population.
  • two SNPs may exist within a gene where each SNP position includes a cytosine variation and an adenine variation.
  • Certain individuals in a population may carry one allele (heterozygous) or two alleles (homozygous) having the gene with a cytosine at each SNP position.
  • the two cytosines corresponding to each SNP in the gene travel together on one or both alleles in these individuals, the individuals can be characterized as having a cytosine/cytosine haplotype with respect to the two SNPs in the gene.
  • phenotype refers to a trait which can be compared between individuals, such as presence or absence of a condition, a visually observable difference in appearance between individuals, metabolic variations, physiological variations, variations in the function of biological molecules, and the like.
  • An example of a phenotype is occurrence of type II diabetes.
  • a polymorphic variant is statistically significant and often biologically relevant if it is represented in 5% or more of a population, sometimes 10% or more, 15% or more, or 20% or more of a population, and often 25% or more, 30% or more, 35% or more, 40% or more, 45% or more, or 50% or more of a population.
  • a polymorphic variant may be detected on either or both strands of a double-stranded nucleic acid.
  • a polymorphic variant may be located within an intron or exon of a gene or within a portion of a regulatory region such as a promoter, a 5 ' untranslated region (UTR), a 3 ' UTR, and in DNA (e.g., genomic DNA (gDNA) and complementary DNA (cDNA)), RNA (e.g., mRNA, tRNA, and rRNA), or a polypeptide.
  • DNA e.g., genomic DNA (gDNA) and complementary DNA (cDNA)
  • RNA e.g., mRNA, tRNA, and rRNA
  • Polymorphic variations may or may not result in detectable differences in gene expression, polypeptide structure, or polypeptide function.
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs456598, rs3798168, rs662138, rs644992, rs637841, rs622342, rs650284 and rs2297374.
  • a guanine at position 24609, a thymine at position 45495, a cytosine at position 50164, a guanine at position 51691, an adenine at position 53067, an adenine at position 58554, an adenine at position 60223, and a thymine at position 61673 were associated with risk of type II diabetes.
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs2195070, rs967507, rs2366577, rs3829747, rs2857265, rs2303539, rs3731750, rs3731749, rs747122, rs2366751, rs3813247, rs3731745, rs3813246, rs3813245, rs744426, rs2042996, rsl560221, rs2288569, rs2288568, rsl001238, rs2288566, rs2288563, rs2243452, rs2246932, rs2562844, rsl 366677, rs2742355, rs2562846, rs2163008, rs2562848, rs2562849, rs2742353, rs
  • Polymorphic variants at the following positions were associated with an increased risk of type ⁇ diabetes: rs2480187, rs6926656, rs2497120, rs2480195, rs6914226, rs6922368, rs2480196, rs2497136, rs2497131, rs2480197, rs3219, rs2497126, rs2480201, rs2476913, rs9449794, rs7741955, and rs2497150.
  • Li the TRPSl locus polymorphic variants at positions selected from the group consisting of rs2625681, rs2625680, rs2625678, rs2625676, rs2737202, rsl905376, rs2178946, rs800919, rs2142327, rs2884365, rs800918, rs800917, rs800916, rs2625683, rs2625682, rsl483583, rs3808432, rsl483584, rsl483585, rs800912, rs3779878, rs3808433, rs3808434, rs3808435, rs3808436, rs3808437, rs3824201, rs3808438, rs3808439, rs3808440, rsl351744, rsl483589, rs
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rsl905376, rs2178946, rsl483583, rs3779878, rs3808435, rs3808437, rs3808438, rs3808440, rsl483589, rs3802219, rs2884366, rs3779879, rs3808447, rs3808448, rs3808450, rs2223103, rsl 160333, rslO28274, rsl963677, rs3779881, rs2358021, rs2049864, rs2049866, rs3808456, rs3808457,,rs719422, rs2178949, rs2272619, rs3808461, rs2737203, rs2737205, rs2721929, r
  • polymorphic variants at positions selected from the group consisting of rsl 1109, rs361737, AA at position 522, rs2236729, rs2236730, rs2051486, rs2051487, rs2051488, rs965764, rs2006783, rs2032416, rs362139, AB at position 19890, rs361901, rs361676, rs362184, rs361797, rs361907, rs362004, rs362176, rs361570, rs361595, rs361778, rs361709, rs361558, rs361762, rs361737, rs362003, rs362011, rs361721, rs361959, rs362230, rs361970, rs362012,
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rsl 1109, rs361709, rs362012, rs362167, rs361976, rs361864, rs362107, rs361940, rs361828, rs361829, rs2156888, rs2156889, rs3827305, rs7104, rsl3604, rslO24372, rs2283799, rs2877079, rs2266991, rs3827306, rs460608, rs457967, rs2236732 and rs715516.
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs374652, rs445865, rs2356537, rs2356538, rs2284243, rs442543, rs397276, rs365745, rs366111, rs2191565, rs381888, rs415913, rs451388, rs430107 and rs2191566.
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs2404167, rs2021681, rsl203868, rs945982, rsl203871, rsl203873, rsl203876, rsl203877, rsl203879, rsl203880, rsl203881, rsl203883, rsl203884, rsl203886, rs2471, rsl203889, rs2277763, rsl203892, rs932560, rsl203894, rsl203896, rsl203898, rsl203899, rsl203905, rsl203907, rsl 1482531, rsl055080, rs2277764, rsl337918, rsl209523, rsl337919, r
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs470780, rs2510345, rsl784500, rs470372, rs470474, rs470536, rs470522, rs470639, rs470565, rs470953, rs639165, rs626717, rs658544, rs470882, rs470425, rs610684, rs625740, rs624504, rs470547, rs470500, rs470713, rs470570, rs470936, rs470600, rs470809, rs595584, rs610382, rs597320, and rs2014708.
  • Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs699493, rs846265, rs7785287, rs846263, rs2282920, rs2330284, rs917229, rs2237422, rs2237420, rsl527499, rs6959829, rs2237415, rs6959294, rs7793034, rsl405750, rs4724092, rs4724093, rs3801168, rslO24552, rs846291, rs846290, rs846315, rs846312, rs846311, rs846310, rs846309, rs846308, rs846306, rs2108165, rs3801174, rs3801177, rs3839733, rs3801183, r
  • methods for identifying a polymorphic variation associated with type II diabetes that is proximal to an incident polymorphic variation associated with type II diabetes which comprises identifying a polymorphic variant proximal to the incident polymorphic variant associated with type II diabetes, where the incident polymorphic variant is in a SLC22A1, TIN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence.
  • the nucleotide sequence often comprises a polynucleotide sequence selected from the group consisting of (a) a polynucleotide sequence of SEQ ID NO: 1-9; (b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence encoded by a polynucleotide sequence of SEQ ID NO: 1-9; and (c) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-9 or a polynucleotide sequence 90% or more identical to the polynucleotide sequence of SEQ ID NO: 1-9.
  • the presence or absence of an association of the proximal polymorphic variant with type II diabetes is determined using a known association method, such as a method described in the Examples hereafter.
  • the incident polymorphic variant is a polymorphic variant associated with type II diabetes described herein.
  • the proximal polymorphic variant identified sometimes is a publicly disclosed polymorphic variant, which for example, sometimes is published in a publicly available database, hi other embodiments, the polymorphic variant identified is not publicly disclosed and is discovered using a known method, including, but not limited to, sequencing a region surrounding the incident polymorphic variant in a group of nucleic samples.
  • a known method including, but not limited to, sequencing a region surrounding the incident polymorphic variant in a group of nucleic samples.
  • the proximal polymorphic variant often is identified in a region surrounding the incident polymorphic variant, hi certain embodiments, this surrounding region is about 50 kb flanking the first polymorphic variant (e.g. about 50 kb 5' of the first polymorphic variant and about 50 kb 3' of the first polymorphic variant), and the region sometimes is composed of shorter flanking sequences, such as flanking sequences of about 40 kb, about 30 kb, about 25 kb, about 20 kb, about 15 kb, about 10 kb, about 7 kb, about 5 kb, or about 2 kb 5' and 3' of the incident polymorphic variant, hi other embodiments, the region is composed of longer flanking sequences, such as flanking sequences of about 55 kb, about 60 kb, about 65 kb, about 70 kb, about 75 kb, about 80 kb, about 85 kb, about 90 kb, about 95 kb, or about 100 kb
  • polymorphic variants associated with type II diabetes are identified iteratively. For example, a first proximal polymorphic variant is associated with type II diabetes using the methods described above and then another polymorphic variant proximal to the first proximal polymorphic variant is identified (e.g., publicly disclosed or discovered) and the presence or absence of an association of one or more other polymorphic variants proximal to the first proximal polymorphic variant with type II diabetes is determined.
  • the methods described herein are useful for identifying or discovering additional polymorphic variants that may be used to further characterize a gene, region or loci associated with a condition, a disease (e.g., type II diabetes), or a disorder.
  • allelotyping or genotyping data from the additional polymorphic variants may be used to identify a functional mutation or a region of linkage disequilibrium.
  • polymorphic variants identified or discovered within a region comprising the first polymorphic variant associated with type II diabetes are genotyped using the genetic methods and sample selection techniques described herein, and it can be determined whether those polymorphic variants are in linkage disequilibrium with the first polymorphic variant.
  • the size of the region in linkage disequilibrium with the first polymorphic variant also can be assessed using these genotyping methods.
  • methods for determining whether a polymorphic variant is in linkage disequilibrium with a first polymorphic variant associated with type II diabetes can be used in prognosis/diagnosis methods described herein.
  • a nucleic acid variant may be represented on one or both strands in a double-stranded nucleic acid or on one chromosomal complement (heterozygous) or both chromosomal complements (homozygous)).
  • nucleic acid includes DNA molecules (e.g., a complementary DNA (cDNA) and genomic DNA (gDNA)) and RNA molecules (e.g., mRNA, rRNA, siRNA and tRNA) and analogs of DNA or RNA, for example, by use of nucleotide analogs.
  • the nucleic acid molecule can be single-stranded and it is often double-stranded.
  • isolated or purified nucleic acid refers to nucleic acids that are separated from other nucleic acids present in the natural source of the nucleic acid.
  • isolated includes nucleic acids which are separated from the chromosome with which the genomic DNA is naturally associated.
  • An "isolated” nucleic acid is often free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and/or 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived.
  • the isolated nucleic acid molecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of 5' and/or 3' nucleotide sequences which flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived.
  • an "isolated" nucleic acid molecule such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized.
  • the term "gene” refers to a nucleotide sequence that encodes a polypeptide.
  • the nucleic acid often comprises a part of or all of a nucleotide sequence in SEQ ID NO: 1-20, or a substantially identical sequence thereof.
  • a nucleotide sequence sometimes is a 5' and/or 3' sequence flanking a polymorphic variant described above that is 5-1000 nucleotides in length, or in some embodiments 5-500, 5-100, 5-75, 5-50, 5-45, 5-40, 5-35, 5-30, 5-25 or 5-20 nucleotides in length.
  • the nucleic acid comprises one or more of the following nucleotides: a guanine at position 522 of SEQ ID NO:5 or a thymine at position 19890 of SEQ ID NO: 5.
  • Other embodiments are directed to methods of identifying a polymorphic variation at one or more positions in a nucleic acid (e.g., genotyping at one or more positions in the nucleic acid), such as at a position corresponding to SNP AA or SNP AB in the PRAME gene.
  • nucleic acid fragments are also included herein. These fragments often are a nucleotide sequence identical to a nucleotide sequence of SEQ ID NO: 1-20, a nucleotide sequence substantially identical to a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence that is complementary to the foregoing.
  • the nucleic acid fragment may be identical, substantially identical or homologous to a nucleotide sequence in an exon or an intron in a nucleotide sequence of SEQ ID NO: 1-9, and may encode a domain or part of a domain of a polypeptide. Sometimes, the fragment will comprises one or more of the polymorphic variations described herein as being associated with type II diabetes.
  • the nucleic acid fragment is often 50, 100, or 200 or fewer base pairs in length, and is sometimes about 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 2000, 3000, 4000, 5000, 10000, 15000, or 20000 base pairs in length.
  • a nucleic acid fragment that is complementary to a nucleotide sequence identical or substantially identical to a nucleotide sequence in SEQ ID-NO: 1-20 and hybridizes to such a nucleotide sequence under stringent conditions is often referred to as a "probe.”
  • Nucleic acid fragments often include one or more polymorphic sites, or sometimes have an end that is adjacent to a polymorphic site as described hereafter.
  • JAM3 nucleic acid fragments sometimes encode an immunoglobulin domain cell adhesion molecule (cam) subfamily region from positions 283 to 534 and 577 to 858 of the mRNA sequence (positions 87 to 170 and 185 to 278, respectively, of the polypeptide sequence) or neural cell adhesion molecule Ll region from positions 340 to 843 of the mRNA sequence (positions 106 to 273 of the polypeptide sequence), for example.
  • cam immunoglobulin domain cell adhesion molecule
  • GLI3 nucleic acid fragments sometimes encode a C2H2-type Zn- finger protein region from positions 1111 to 1935 of the mRNA sequence (positions 353 to 627 of the polypeptide sequence) or a zinc finger region from positions 1492 to 1950 of the mRNA sequence (positions 480 to 632 of the polypeptide sequence), for example.
  • An example of a nucleic acid fragment is an oligonucleotide.
  • oligonucleotide refers to a nucleic acid comprising about 8 to about 50 covalently linked nucleotides, often comprising from about 8 to about 35 nucleotides, and more often from about 10 to about 25 nucleotides.
  • oligonucleotide may be the same as those of naturally occurring nucleic acids, or analogs or derivatives of naturally occurring nucleic acids, provided that oligonucleotides having such analogs or derivatives retain the ability to hybridize specifically to a nucleic acid comprising a targeted polymorphism. Oligonucleotides described herein may be used as hybridization probes or as components of prognostic or diagnostic assays, for example, as described herein.
  • Oligonucleotides are typically synthesized using standard methods and equipment, such as the ABF M 3900 High Throughput DNA Synthesizer and the EXPEDITETM 8909 Nucleic Acid Synthesizer, both of which are available from Applied Biosystems (Foster City, CA). Analogs and derivatives are exemplified in U.S. Pat. Nos.
  • Oligonucleotides may also be linked to a second moiety.
  • the second moiety may be an additional nucleotide sequence such as a tail sequence (e.g., a polyadenosine tail), an adapter sequence (e.g., phage Ml 3 universal tail sequence), and others.
  • the second moiety may be a non-nucleotide moiety such as a moiety which facilitates linkage to a solid support or a label to facilitate detection of the oligonucleotide.
  • labels include, without limitation, a radioactive label, a fluorescent label, a chemiluminescent label, a paramagnetic label, and the like.
  • the second moiety may be attached to any position of the oligonucleotide, provided the oligonucleotide can hybridize to the nucleic acid comprising the polymorphism.
  • Nucleic acid coding sequences may be used for diagnostic purposes for detection and control of polypeptide expression.
  • oligonucleotide sequences such as antisense RNA, small-interfering RNA (siRNA) and DNA molecules and ribozymes that function to inhibit translation of a polypeptide.
  • Antisense techniques and RNA interference techniques are known in the art and are described herein.
  • Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA.
  • the mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage.
  • hammerhead motif ribozyme molecules may be engineered that specifically and efficiently catalyze endonucleolytic cleavage of RNA sequences corresponding to or complementary to SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequences.
  • ribozyme cleavage sites within any potential RNA target are initially identified by scanning the target molecule for ribozyme cleavage sites which include the following sequences, GUA, GUU and GUC. Once identified, short RNA sequences of between fifteen (15) and twenty (20) ribonucleotides corresponding to the region of the target gene containing the cleavage site may be evaluated for predicted structural features such as secondary structure that may render the oligonucleotide sequence unsuitable. The suitability of candidate targets may also be evaluated by testing their accessibility to hybridization with complementary oligonucleotides, using ribonuclease protection assays.
  • Antisense RNA and DNA molecules, siRNA and ribozymes may be prepared by any method known in the art for the synthesis of RNA molecules. These include techniques for chemically synthesizing oligodeoxyribonucleotides well known in the art such as solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into cell lines.
  • DNA encoding a polypeptide also may have a number of uses for the diagnosis of diseases, including type II diabetes, resulting from aberrant expression of a target gene described herein.
  • the nucleic acid sequence may be used in hybridization assays of biopsies or autopsies to diagnose abnormalities of expression or function (e.g., Southern or Northern blot analysis, in situ hybridization assays).
  • the expression of a polypeptide during embryonic development may also be determined using nucleic acid encoding the polypeptide.
  • production of functionally impaired polypeptide is the cause of various disease states, such as type II diabetes.
  • In situ hybridizations using polypeptide as a probe may be employed to predict problems related to type II diabetes.
  • administration of human active polypeptide, recombinantly produced as described herein may be used to treat disease states related to functionally impaired polypeptide.
  • gene therapy approaches may be employed to remedy deficiencies of functional polypeptide or to replace or compete with dysfunctional polypeptide.
  • nucleic acid vectors often expression vectors, which contain a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLIS nucleotide sequence or a substantially identical sequence thereof.
  • vector refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked and can include a plasmid, cosmid, or viral vector.
  • the vector can be capable of autonomous replication or it can integrate into a host DNA.
  • Viral vectors may include replication defective retroviruses, adenoviruses and adeno-associated viruses for example.
  • a vector can include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLI3 nucleotide sequence in a form suitable for expression of an encoded target polypeptide or target nucleic acid in a host cell.
  • a "target polypeptide” is a polypeptide encoded by & SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence or a substantially identical nucleotide sequence thereof.
  • the recombinant expression vector typically includes one or more regulatory sequences operatively linked to the nucleic acid sequence to be expressed.
  • regulatory sequence includes promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence, as well as tissue-specific regulatory and/or inducible sequences.
  • the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, and the like. Expression vectors can be introduced into host cells to produce target polypeptides, including fusion polypeptides.
  • Recombinant expression vectors can be designed for expression of target polypeptides in prokaryotic or eukaryotic cells.
  • target polypeptides can be expressed in E. coli, insect cells (e.g., using baculovirus expression vectors), yeast cells, or mammalian cells. Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990).
  • the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.
  • Fusion vectors add a number of amino acids to a polypeptide encoded therein, usually to the amino terminus of the recombinant polypeptide.
  • Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant polypeptide; 2) to increase the solubility of the recombinant polypeptide; and 3) to aid in the purification of the recombinant polypeptide by acting as a ligand in affinity purification.
  • a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant polypeptide to enable separation of the recombinant polypeptide from the fusion moiety subsequent to purification of the fusion polypeptide.
  • enzymes, and their cognate recognition sequences include Factor Xa, thrombin and enterokinase.
  • Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith & Johnson, Gene 67: 31-40 (1988)), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding polypeptide, or polypeptide A, respectively, to the target recombinant polypeptide.
  • GST glutathione S-transferase
  • fusion polypeptides can be used in screening assays and to generate antibodies specific for target polypeptides.
  • fusion polypeptide expressed in a retroviral expression vector is used to infect bone marrow cells that are subsequently transplanted into irradiated recipients. The pathology of the subject recipient is then examined after sufficient time has passed ⁇ e.g., six (6) weeks).
  • the expression vector's control functions are often provided by viral regulatory elements.
  • viral regulatory elements For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40.
  • Recombinant mammalian expression vectors are often capable of directing expression of the nucleic acid in a particular cell type (e.g.', tissue-specific regulatory elements are used to express the nucleic acid).
  • tissue-specific promoters include an albumin promoter (liver-specific; Pinkert et al, Genes Dev. 1: 268-277 (1987)), lymphoid-specific promoters (Calame & Eaton, Adv. Immunol.
  • promoters of T cell receptors (Winoto & Baltimore, EMBO J. 8: 729-733 (1989)) promoters of immunoglobulins (Banerji et al, Cell 33: 729-740 (1983); Queen & Baltimore, Cell 33: 741-948 (1983)), neuron-specific promoters (e.g., the neurofilament promoter; Byrne & Ruddle, Proc. Natl. Acad. Sci.
  • pancreas-specific promoters Eslund et al, Science 230: 912-916 (1985)
  • mammary gland-specific promoters e.g., milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166.
  • Developmentally-regulated promoters are sometimes utilized, for example, the murine hox promoters (Kessel & Grass, Science 249: 31A-119 (1990)) and the ⁇ -fetopolypeptide promoter (Campes & Tilghman, Genes Dev. 3: 537-546 (1989)).
  • a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid may also be cloned.into an expression vector in an antisense orientation.
  • Regulatory sequences e.g., viral promoters and/or enhancers
  • operatively linked to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid cloned in the antisense orientation can be chosen for directing constitutive, tissue specific or cell type specific expression of antisense RNA in a variety of cell types.
  • Antisense expression vectors can be in the form of a recombinant plasmid, phagemid or attenuated virus.
  • Antisense genes see, e.g., Weintraub et ah, Antisense RNA as a molecular tool for genetic analysis, Reviews - Trends in Genetics, Vol. 1(1) (1986).
  • host cells that include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLI3 nucleotide sequence within a recombinant expression vector or a fragment of such a nucleotide sequence which facilitate homologous recombination into a specific site of the host cell genome.
  • host cell and “recombinant host cell” are used interchangeably herein. Such terms refer not only to the particular subject cell but rather also to the progeny or potential progeny of such a cell.
  • a host cell can be any prokaryotic or eukaryotic cell.
  • a target polypeptide can be expressed in bacterial cells such as E. coli, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells).
  • bacterial cells such as E. coli, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells).
  • mammalian cells such as Chinese hamster ovary cells (CHO) or COS cells.
  • Other suitable host cells are known to those skilled in the art.
  • Vectors can be introduced into host cells via conventional transformation or transfection techniques.
  • a host cell provided herein can be used to produce (i.e., express) a target polypeptide or a substantially identical polypeptide thereof. Accordingly, further provided are methods for producing a target polypeptide using host cells described herein. In one embodiment, the method includes culturing host cells into which a recombinant expression vector encoding a target polypeptide has been introduced in a suitable medium such that a target polypeptide is produced. In another embodiment, the method further includes isolating a target polypeptide from the medium or the host cell.
  • cells or purified preparations of cells which include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB transgene, or which otherwise misexpress target polypeptide.
  • Cell preparations can consist of human or non-human cells, e.g., rodent cells, e.g., mouse or rat cells, rabbit cells, or pig cells.
  • the cell or cells include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB transgene (e.g., a heterologous form of a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLL3 gene, such as a human gene expressed in non-human cells).
  • the transgene can be misexpressed, e.g., overexpressed or underexpressed.
  • the cell or cells include a gene which misexpress an endogenous target polypeptide (e.g., expression of a gene is disrupted, also known as a knockout).
  • a gene which misexpress an endogenous target polypeptide e.g., expression of a gene is disrupted, also known as a knockout.
  • Such cells can serve as a model for studying disorders which are related to mutated or mis-expressed alleles or for use in drug screening.
  • human cells e.g., a hematopoietic stem cells transformed with a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid.
  • cells or a purified preparation thereof e.g. , human cells
  • an endogenous SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid is under the control of a regulatory sequence that does not normally control the expression of the endogenous gene corresponding to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequence.
  • an endogenous gene within a cell can be modified by inserting a heterologous DNA regulatory element into the genome of the cell such that the inserted regulatory element is operably linked to the corresponding endogenous gene.
  • a heterologous DNA regulatory element e.g., a gene which is "transcriptionally silent,” not normally expressed, or expressed only at very low levels
  • an endogenous corresponding gene may be activated by inserting a regulatory element which is capable of promoting the expression of a normally expressed gene product in that cell.
  • Techniques such as targeted homologous recombinations, can be used to insert the heterologous DNA as described in, e.g., Chappel, US 5,272,071; WO 91/06667, published on May 16, 1991.
  • Non-human transgenic animals that express a heterologous target polypeptide (e.g., expressed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid or substantially identical sequence thereof) can be generated. Such animals are useful for studying the function and/or activity of a target polypeptide and for identifying and/or evaluating modulators of the activity of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acids and encoded polypeptides.
  • a heterologous target polypeptide e.g., expressed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acids and encoded polypeptides.
  • a "transgenic animal” is a non-human animal such as a mammal (e.g., a non-human primate such as chimpanzee, baboon, or macaque; an ungulate such as an equine, bovine, or caprine; or a rodent such as a rat, a mouse, or an Israeli sand rat), a bird ⁇ e.g., a chicken or a turkey), an amphibian (e.g., a frog, salamander, or newt), or an insect (e.g., Drosophil ⁇ mel ⁇ nog ⁇ stef), in which one or more of the cells of the animal includes a transgene.
  • a mammal e.g., a non-human primate such as chimpanzee, baboon, or macaque
  • an ungulate such as an equine, bovine, or caprine
  • a rodent such as a rat, a mouse, or
  • a transgene is exogenous DNA or a rearrangement (e.g., a deletion of endogenous chromosomal DNA) that is often integrated into or occurs in the genome of cells in a transgenic animal.
  • a transgene can direct expression of an encoded gene product in one or more cell types or tissues of the transgenic animal, and other transgenes can reduce expression (e.g., a knockout).
  • a transgenic animal can be one in which an endogenous nucleic acid homologous to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal (e.g., an embryonic cell of the animal) prior to development of the animal.
  • a cell of the animal e.g., an embryonic cell of the animal
  • Intronic sequences and polyadenylation signals can also be included in the transgene to increase expression efficiency of the transgene.
  • One or more tissue-specific regulatory sequences can be operably linked to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence to direct expression of an encoded polypeptide to particular cells.
  • a transgenic founder animal can be identified based upon the presence of a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence in its genome and/or expression of encoded mRNA in tissues or cells of the animals.
  • transgenic founder animal can then be used to breed additional animals carrying the transgene.
  • transgenic animals carrying a SLC22A1, TTN, LOCI 12609, TRPSl, FRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence can further be bred to other transgenic animals carrying other transgenes.
  • Target polypeptides can be expressed in transgenic animals or plants by introducing, for example, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid into the genome of an animal that encodes the target polypeptide.
  • the nucleic acid is placed under the control of a tissue specific promoter, e.g., a milk or egg specific promoter, and recovered from the milk or eggs produced by the animal.
  • tissue specific promoter e.g., a milk or egg specific promoter
  • a population of cells from a transgenic animal e.g., a milk or egg specific promoter
  • isolated target polypeptides which are encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence (e.g., SEQ ID NO: 1-20) or a substantially identical nucleotide sequence thereof, such as the polypeptides having amino acid sequences in SEQ ID NO: 21-30.
  • polypeptide as used herein includes proteins and peptides.
  • An “isolated” or “purified” polypeptide or protein is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized.
  • the language "substantially free” means preparation of a target polypeptide having less than about 30%, 20%, 10% and more preferably 5% (by dry weight), of non-target polypeptide (also referred to herein as a "contaminating protein"), or of chemical precursors or non-target chemicals.
  • the target polypeptide or a biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, specifically, where culture medium represents less than about 20%, sometimes less than about 10%, and often less than about 5% of the volume of the polypeptide preparation.
  • Isolated or purified 1 target polypeptide preparations are sometimes 0.01 milligrams or more or 0.1 milligrams or more, and often 1.0 milligrams or more and 10 milligrams or more in dry weight.
  • the polypeptide fragment may be a domain or part of a domain of a target polypeptide.
  • a fragment sometimes is a FOXA2 polypeptide domain encoded by a nucleotide sequence referenced in SEQ ID NO: 17 or 18.
  • the polypeptide fragment may have increased, decreased or unexpected biological activity.
  • the polypeptide fragment is often 50 or fewer, 100 or fewer, or 200 or fewer amino acids in length, and is sometimes 300, 400, 500, 600, 700, or 900 or fewer amino acids in length.
  • Substantially identical target polypeptides may depart from the amino acid sequences of target polypeptides in different manners. For example, conservative amino acid modifications may be introduced at one or more positions in the amino acid sequences of target polypeptides.
  • a "conservative amino acid substitution” is one in which the amino acid is replaced by another amino acid having a similar structure and/or chemical function. Families of amino acid residues having similar structures and functions are well known.
  • amino acids with basic side chains ⁇ e.g., lysine, arginine, histidine
  • acidic side chains e.g., aspartic acid, glutamic acid
  • uncharged polar side chains e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine
  • nonpolar side chains e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan
  • beta-branched side chains e.g., threonine, valine, isoleucine
  • aromatic side chains e.g., tyrosine, phenylalanine, tryptophan, histidine
  • non-essential amino acids may be replaced.
  • a "non-essential" amino acid is one that can be altered without abolishing or substantially altering the biological function of a target polypeptide, whereas altering an "essential” amino acid abolishes or substantially alters the biological function of a target polypeptide.
  • Amino acids that are conserved among target polypeptides are typically essential amino acids.
  • target polypeptides may exist as chimeric or fusion polypeptides.
  • a target "chimeric polypeptide” or target “fusion polypeptide” includes a target polypeptide linked to a non-target polypeptide.
  • a "non-target polypeptide” refers to a polypeptide having an amino acid sequence corresponding to a polypeptide which is not substantially identical to the target polypeptide, which includes, for example, a polypeptide that is different from the target polypeptide and derived from the same or a different organism.
  • the target polypeptide in the fusion polypeptide can correspond to an entire or nearly entire target polypeptide or a fragment thereof.
  • the non-target polypeptide can be fused to the N-terminus or C-terminus of the target polypeptide.
  • Fusion polypeptides can include a moiety having high affinity for a ligand.
  • the fusion polypeptide can be a GST-target fusion polypeptide in which the target sequences are fused to the C-terminus of the GST sequences, or a polyhistidine-target fusion polypeptide in which the target polypeptide is fused at the N- or C-terminus to a string of histidine residues.
  • Such fusion polypeptides can facilitate purification of recombinant target polypeptide.
  • Fusion polypeptides are commercially available that already encode a fusion moiety (e.g., a GST polypeptide), and a nucleotide sequence in SEQ ID NO: 1-20, or a substantially identical nucleotide sequence thereof, can be cloned into an expression vector such that the fusion moiety is linked in- frame to the target polypeptide.
  • the fusion polypeptide can be a target polypeptide containing a heterologous signal sequence at its N-terminus.
  • expression, secretion, cellular internalization, and cellular localization of a target polypeptide can be increased through use of a heterologous signal sequence.
  • Fusion polypeptides can also include all or a part of a serum polypeptide (e.g., an IgG constant region or human serum albumin).
  • Target polypeptides can be incorporated into pharmaceutical compositions and administered to a subject in vivo. Administration of these target polypeptides can be used to affect the bioavailability of a substrate of the target polypeptide and may effectively increase target polypeptide biological activity in a cell.
  • Target fusion polypeptides may be useful therapeutically for the treatment of disorders caused by, for example, (i) aberrant modification or mutation of a gene encoding a target polypeptide; (ii) mis-regulation of the gene encoding the target polypeptide; and (iii) aberrant post-translational modification of a target polypeptide.
  • target polypeptides can be used as immunogens to produce anti-target antibodies in a subject, to purify target polypeptide ligands or binding partners, and in screening assays to identify molecules which inhibit or enhance the interaction of a target polypeptide with a substrate.
  • polypeptides can be chemically synthesized using techniques known in the art (See, e.g., Creighton, 1983 Proteins. New York, N.Y.: W. H. Freeman and Company; and Hunkapiller et al, (1984) Nature July 12 -18;310(5973):105-ll).
  • a relative short fragment can be synthesized by use of a peptide synthesizer.
  • non-classical amino acids or chemical amino acid analogs can be introduced as a substitution or addition into the fragment sequence.
  • Non-classical amino acids include, but are not limited to, to the D-isomers of the common amino acids, 2,4-diaminobutyric acid, a-amino isobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, g-Abu, e-Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3- amino propionic acid, ornithine, norleucine, norvaline, hydroxyproline, sarcosine, citrulline, homocitrulline, cysteic acid, t-butylglycine, t-butylalanine, phenylglycine, cyclohexylalanine, b- alanine, fluoroamino acids, designer amino acids such as b-methyl amino acids, Ca-methyl amino acids, Na-methyl amino acids, and amino acid analogs in general. Furthermore, the amino acid can be
  • Polypeptides and polypeptide fragments sometimes are differentially modified during or after translation, e.g., by glycosylation, acetylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to an antibody molecule or other cellular ligand, etc. Any of numerous chemical modifications may be carried out by known techniques, including but not limited, to specific chemical cleavage by cyanogen bromide, trypsin, chymotrypsin, papain, V8 protease, NaBH4; acetylation, formylation, oxidation, reduction; metabolic synthesis in the presence of tunicamycin; and the like.
  • Additional post-translational modifications include, for example, N-linked or O-linked carbohydrate chains, processing of N- terminal or C-terminal ends), attachment of chemical moieties to the amino acid backbone, chemical modifications of N-linked or O-linked carbohydrate chains, and addition or deletion of an N-terminal methionine residue as a result of prokaryotic host cell expression.
  • the polypeptide fragments may also be modified with a detectable label, such as an enzymatic, fluorescent, isotopic or affinity label to allow for detection and isolation of the polypeptide.
  • chemically modified derivatives of polypeptides that can provide additional advantages such as increased solubility, stability and circulating time of the polypeptide, or decreased immunogenicity (see e.g., U.S. Pat. No: 4,179,337.
  • the chemical moieties for derivitization may be selected from water soluble polymers such as polyethylene glycol, ethylene glycol/propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol and the like.
  • the polypeptides may be modified at random positions within the molecule, or at predetermined positions within the molecule and may include one, two, three or more attached chemical moieties.
  • the polymer may be of any molecular weight, and may be branched or unbranched.
  • the preferred molecular weight is between about 1 kDa and about 100 kDa (the term "about” indicating that in preparations of polyethylene glycol, some molecules will weigh more, some less, than the stated molecular weight) for ease in handling and manufacturing.
  • Other sizes may be used, depending on the desired therapeutic profile (e.g., the duration of sustained release desired, the effects, if any on biological activity, the ease in handling, the degree or lack of antigenicity and other known effects of the polyethylene glycol to a therapeutic protein or analog).
  • polymers should be attached to the polypeptide with consideration of effects on functional or antigenic domains of the polypeptide.
  • attachment methods available to those skilled in the art (e.g., EP 0 401 384 (coupling PEG to G-CSF) and Malik et al. (1992) Exp Hematol. September;20(8): 1028-35 (pegylation of GM-CSF using tresyl chloride)).
  • polyethylene glycol may be covalently bound through amino acid residues via a reactive group, such as a free amino or carboxyl group.
  • Reactive groups are those to which an activated polyethylene glycol molecule may be bound.
  • the amino acid residues having a free amino group may include lysine residues and the N-terminal amino acid residues; those having a free carboxyl group may include aspartic acid residues, glutamic acid residues and the C-terminal amino acid residue.
  • Sulfhydryl groups may also be used as a reactive group for attaching the polyethylene glycol molecules.
  • the attachment sometimes is at an amino group, such as attachment at the N-terminus or lysine group.
  • Proteins can be chemically modified at the N-terminus.
  • polyethylene glycol as an illustration of such a composition, one may select from a variety of polyethylene glycol molecules (by molecular weight, branching, and the like), the proportion of polyethylene glycol molecules to protein (polypeptide) molecules in the reaction mix, the type of pegylation reaction to be performed, and the method of obtaining the selected N-terminally pegylated protein.
  • the method of obtaining the N-terminally pegylated preparation i.e., separating this moiety from other monopegylated moieties if necessary
  • Selective proteins chemically modified at the N- terminus may be accomplished by reductive alkylation, which exploits differential reactivity of different types of primary amino groups (lysine versus the N-terminal) available for derivatization in a particular protein. Under the appropriate reaction conditions, substantially selective derivatization of the protein at the N-terminus with a carbonyl group containing polymer is achieved.
  • nucleotide sequences and polypeptide sequences that are substantially identical to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence and the target polypeptide sequences encoded by those nucleotide sequences, respectively, are included herein.
  • the term "substantially identical” as used herein refers to two or more nucleic acids or polypeptides sharing one or more identical nucleotide sequences or polypeptide sequences, respectively.
  • nucleotide sequences or polypeptide sequences that are 55% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more (each often within a 1%, 2%, 3% or 4% variability) identical to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence or the encoded target polypeptide amino acid sequences.
  • One test for determining whether two nucleic acids are substantially identical is to determine the percent of identical nucleotide sequences or polypeptide sequences shared between the nucleic acids or polypeptides.
  • sequence identity is often performed as follows. Sequences are aligned for optimal comparison purposes (e.g. , gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes).
  • the length of a reference sequence aligned for comparison purposes is sometimes 30% or more, 40% or more, 50% or more, often 60% or more, and more often 70% or more, 80% or more, 90% or more, or 100% of the length of the reference sequence.
  • the nucleotides or amino acids at corresponding nucleotide or polypeptide positions, respectively, are then compared among the two sequences.
  • the nucleotides or amino acids are deemed to be identical at that position.
  • the percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, introduced for optimal alignment of the two sequences.
  • Comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. Percent identity between two amino acid or nucleotide sequences can be determined using the algorithm of Meyers & Miller, CABIOS 4: 11-17 (1989), which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. Also, percent identity between two amino acid sequences can be determined using the Needleman & Wunsch, J. MoI. Biol.
  • nucleic acids Another manner for determining if two nucleic acids are substantially identical is to assess whether a polynucleotide homologous to one nucleic acid will hybridize to the other nucleic acid under stringent conditions.
  • stringent conditions refers to conditions for hybridization and washing. Stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. , 6.3.1-6.3.6 (1989). Aqueous and non-aqueous methods are described in that reference and either can be used.
  • stringent hybridization conditions is hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 50 0 C.
  • Another example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 55°C.
  • a further example of stringent hybridization conditions is hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45 0 C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 60 0 C.
  • stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 65°C. More often, stringency conditions are 0.5M sodium phosphate, 7% SDS at 65°C, followed by one or more washes at 0.2X SSC, 1% SDS at 65°C.
  • SSC sodium chloride/sodium citrate
  • An example of a substantially identical nucleotide sequence to a nucleotide sequence in SEQ ID NO: 1-20 is one that has a different nucleotide sequence but still encodes the same polypeptide sequence encoded by the nucleotide sequence in SEQ ED NO: 1-20.
  • Another example is a nucleotide sequence that encodes a polypeptide having a polypeptide sequence that is more than 70% or more identical to, sometimes more than 75% or more, 80% or more, or 85% or more identical to, and often more than 90% or more and 95% or more identical to a polypeptide sequence encoded by a nucleotide sequence in SEQ ID NO: 1-20.
  • SEQ ID NO: 1-20 typically refers to one or more sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20. Many of the embodiments described herein are applicable to (a) a nucleotide sequence of SEQ DO NO: 1-20; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; (d) a fragment of a nucleotide sequence of (a), (b), or (c); and/or a nucleotide sequence complementary to the nucleo
  • nucleotide sequences examples include nucleotide sequences from subjects that differ by naturally occurring genetic variance, which sometimes is referred to as background genetic variance ⁇ e.g., nucleotide sequences differing by natural genetic variance sometimes are 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to one another).
  • Gapped BLAST can be utilized as described in Altschul et al, Nucleic Acids Res. 25(17): 3389- 3402 (1997).
  • default parameters of the respective programs e.g., XBLAST and NBLAST
  • a nucleic acid that is substantially identical to a nucleotide sequence in SEQ ID NO: 1- 20 may include polymorphic sites at positions equivalent to those described herein when the sequences are aligned.
  • SNPs in a sequence substantially identical to a sequence in SEQ ID NO: 1-20 can be identified at nucleotide positions that match with or correspond to (i.e., align) nucleotides at SNP positions in each nucleotide sequence in SEQ ID NO: 1-20.
  • insertion or deletion of a nucleotide sequence from a reference sequence can change the relative positions of other polymorphic sites in the nucleotide sequence.
  • Substantially identical nucleotide and polypeptide sequences include those that are naturally occurring, such as allelic variants (same locus), splice variants, homologs (different locus), and orthologs (different organism) or can be non-naturally occurring.
  • Non-naturally occurring variants can be generated by mutagenesis techniques, including those applied to polynucleotides, cells, or organisms.
  • the variants can contain nucleotide substitutions, deletions, inversions and insertions. Variation can occur in either or both the coding and non-coding regions. The variations can produce both conservative and non-conservative amino acid substitutions (as compared in the encoded product).
  • Orthologs, homologs, allelic variants, and splice variants can be identified using methods known in the art. These variants normally comprise a nucleotide sequence encoding a polypeptide that is 50% or more, about 55% or more, often about 70-75% or more or about 80-85% or more, and sometimes about 90-95% or more identical to the amino acid sequences of target polypeptides or a fragment thereof. Such nucleic acid molecules can readily be identified as being able to hybridize under stringent conditions to a nucleotide sequence in SEQ ID NO: 1-20 or a fragment of this sequence.
  • nucleic acid molecules corresponding to orthologs, homologs, and allelic variants of a nucleotide sequence in SEQ ID NO: 1-20 can further be identified by mapping the sequence to the same chromosome or locus as the nucleotide sequence in SEQ ID NO: 1-20.
  • substantially identical nucleotide sequences may include codons that are altered with respect to the naturally occurring sequence for enhancing expression of a target polypeptide in a particular expression system.
  • the nucleic acid can be one in which one OF more codons are altered, and often 10% or more or 20% or more of the codons are altered for optimized expression in bacteria (e.g., E. coli.), yeast (e.g., S. cervesiae), human (e.g., 293 cells), insect or rodent (e.g., hamster)- cells.
  • nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO: 1-20; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence
  • polymorphic variants at the positions described herein are detected for determining a risk of type II diabetes, and polymorphic variants at positions in linkage disequilibrium with these positions are detected for determining a risk of type II diabetes.
  • SEQ ID NO: 1-20 refers to individual sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20.
  • Results from prognostic tests may be combined with other test results to diagnose type II diabetes related disorders, including metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia.
  • prognostic results may be gathered, a patient sample may be ordered based on a determined predisposition to type II diabetes, the patient sample is analyzed, and the results of the analysis may be utilized to diagnose the type II diabetes related condition (e.g., metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia).
  • type II diabetes diagnostic methods can be developed from studies used to generate prognostic methods in which populations are stratified into subpopulations having different progressions of a type II diabetes related disorder or condition.
  • prognostic results may be gathered, a patient's risk factors for developing type II diabetes (e.g., age, weight, race, dief ⁇ analyzed, and a patient sample may be ordered based on a determined predisposition to type II diabetes.
  • a patient's risk factors for developing type II diabetes e.g., age, weight, race, dief ⁇ analyzed
  • a patient sample may be ordered based on a determined predisposition to type II diabetes.
  • Risk of type II diabetes sometimes is expressed as a probability, such as an odds ratio, percentage, or risk factor.
  • the risk sometimes is expressed as a relative risk with respect to a population average risk of type II diabetes, and sometimes is expressed as a relative risk with respect to the lowest risk group.
  • Such relative risk assessments often are based upon penetrance values determined by statistical methods and are particularly useful to clinicians and insurance companies for assessing risk of type II diabetes (e.g., a clinician can target appropriate detection, prevention and therapeutic regimens to a patient after determining the patient's risk of type II diabetes, and an insurance company can fine tune actuarial tables based upon population genotype assessments of type II diabetes risk).
  • Risk of type II diabetes sometimes is expressed as an odds ratio, which is the odds of a particular person having a genotype has or will develop type II diabetes with respect to another genotype group (e.g., the most disease protective genotype or population average).
  • the risk often is based upon the presence or absence of one or more polymorphic variants described herein, and also may be based in part upon phenotypic traits of the individual being tested.
  • two or more polymorphic variations are detected in a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB locus.
  • nucleic acid sample typically is isolated from a biological sample obtained from a subject.
  • nucleic acid can be isolated from blood, saliva, sputum, urine, cell scrapings, and biopsy tissue.
  • the nucleic acid sample can be isolated from a biological sample using standard techniques, such as the technique described in Example 2.
  • the term "subject” refers primarily to humans but also refers to other mammals such as dogs, cats, and ungulates (e.g., cattle, sheep, and swine). Subjects also include avians (e.g., chickens and turkeys ⁇ , reptiles, and fish (e.g., salmon), as embodiments described herein can be adapted to nucleic acid samples isolated from any of these organisms.
  • the nucleic acid sample may be isolated from the subject and then directly utilized in a method for determining the presence of a polymorphic variant, or alternatively, the sample may be isolated and then stored (e.g., frozen) for a period of time before being subjected to analysis.
  • the presence or absence of a polymorphic variant is determined using one or both chromosomal complements represented in the nucleic acid sample. Determining the presence or absence of a polymorphic variant in both chromosomal complements represented in a nucleic acid sample from a subject having a copy of each chromosome is useful for determining the zygosity of an individual for the polymorphic variant (i.e., whether the individual is homozygous or heterozygous for the polymorphic variant). Any oligonucleotide-based diagnostic may be utilized to determine whether a sample includes the presence or absence of a polymorphic variant in a sample. For example, primer extension methods, ligase sequence determination methods (e.g., U.S.
  • mismatch sequence dete ⁇ nination methods e.g., U.S. Pat. Nos. 5,851,770; 5,958,692; 6,110,684; and 6,183,958
  • microarray sequence determination methods e.g., restriction fragment length polymorphism (RFLP), single strand conformation polymorphism detection (SSCP) (e.g., U.S. Pat. Nos. 5,891,625. and 6,013,499), PCR- based assays (e.g., TAQMAN ® PCR System (Applied Biosystems)), and nucleotide sequencing methods may be used.
  • RFLP restriction fragment length polymorphism
  • SSCP single strand conformation polymorphism detection
  • PCR- based assays e.g., TAQMAN ® PCR System (Applied Biosystems)
  • nucleotide sequencing methods may be used.
  • Oligonucleotide extension methods typically involve providing a pair of oligonucleotide primers in a polymerase chain reaction (PCR) or in other nucleic acid amplification methods for the purpose of amplifying a region from the nucleic acid sample that comprises the polymorphic variation.
  • PCR polymerase chain reaction
  • One oligonucleotide primer is complementary to a region 3' of the polymorphism and the other is complementary to a region 5' of the polymorphism.
  • a PCR primer pair may be used in methods disclosed in U.S. Pat. Nos. 4,683,195; 4,683,202, 4,965,188; 5,656,493; 5,998,143; 6,140,054; WO 01/27327; and WO 01/27329 for example.
  • PCR primer pairs may also be used in any commercially available machines that perform PCR, such as any of the GENEAMP ® Systems available from Applied Biosystems. Also, those of ordinary skill in the art will be able to design oligonucleotide primers based upon a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence using knowledge available in the art.
  • extension oligonucleotide that hybridizes to the amplified fragment adjacent to the polymorphic variation.
  • adjacent refers to the 3' end of the extension oligonucleotide being often 1 nucleotide from the 5' end of the polymorphic site, and sometimes 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides from the 5' end of the polymorphic site, in the nucleic acid when the extension oligonucleotide is hybridized to the nucleic acid.
  • extension oligonucleotide then is extended by one or more nucleotides, and the number and/or type of nucleotides that are added to the extension oligonucleotide determine whether the polymorphic variant is present.
  • Oligonucleotide extension methods are disclosed, for example, in U.S. Pat. Nos. 4,656,127; 4,851,331; 5,679,524; 5,834,189; 5,876,934; 5,908,755; 5,912,118; 5,976,802; 5,981,186; 6,004,744; 6,013,431; 6,017,702; 6,046,005; 6,087,095; 6,210,891; and WO 01/20039.
  • Oligonucleotide extension methods using mass spectrometry are described, for example, in U.S. Pat. Nos. 5,547,835; 5,605,798; 5,691,141; 5,849,542; 5,869,242; 5,928,906; 6,043,031; and 6,194,144, and a method often utilized is described herein in Example 2.
  • a microarray can be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample.
  • a microarray may include any oligonucleotides described herein, and methods for making and using oligonucleotide microarrays suitable for diagnostic use are disclosed in U.S. Pat. Nos.
  • the microarray typically comprises a solid support and the oligonucleotides may be linked to this solid support by covalent bonds or by non-covalent interactions.
  • the oligonucleotides may also be linked to the solid support directly or by a spacer molecule.
  • a microarray may comprise one or more oligonucleotides complementary to a polymorphic site set forth herein.
  • a kit also may be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample.
  • a kit often comprises one or more pairs of oligonucleotide primers useful for amplifying a fragment of a nucleotide sequence of SEQ ID NO: 1-20 or a substantially identical sequence thereof, where the fragment includes a polymorphic site.
  • the kit sometimes comprises a polymerizing agent, for example, a thermostable nucleic acid polymerase such as one disclosed in U.S. Pat. Nos. 4,889,818 or 6,077,664.
  • the kit often comprises an elongation oligonucleotide that hybridizes to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence in a nucleic acid sample adjacent to the polymorphic site.
  • the kit includes an elongation oligonucleotide, it also often comprises chain elongating nucleotides, such as dATP, dTTP, dGTP, dCTP, and dITP, including analogs of dATP, dTTP, dGTP, dCTP and dITP, provided that such analogs are substrates for a thermostable nucleic acid polymerase and can be incorporated into a nucleic acid chain elongated from the extension oligonucleotide.
  • chain elongating nucleotides would be one or more chain terminating nucleotides such as ddATP, ddTTP, ddGTP, ddCTP, and the like.
  • the kit comprises one or more oligonucleotide primer pairs, a polymerizing agent, chain elongating nucleotides, at least one elongation oligonucleotide, and one or more chain terminating nucleotides.
  • Kits optionally include buffers, vials, microtiter plates, and instructions for use.
  • An individual identified as being at risk of type II diabetes may be heterozygous or homozygous with respect to the allele associated with a higher risk of type II diabetes.
  • a subject homozygous for an allele associated with an increased risk of type II diabetes is at a comparatively high risk of type II diabetes
  • a subject heterozygous for an allele associated with an increased risk of type II diabetes is at a comparatively intermediate risk of type II diabetes
  • a subject homozygous for an allele associated with a decreased risk of type II diabetes is at a comparatively low risk of type II diabetes.
  • a genotype may be assessed for a complementary strand, such that the complementary nucleotide at a particular position is detected.
  • the antibody specifically binds to an epitope comprising a glycine at position 557 in a ZNF221 polypeptide.
  • Pharmacogenomics is a discipline that involves, tailoring a treatment for a subject according to the subject's genotype as a particular treatment regimen may exert a differential effect depending upon the subject's genotype. For example, based upon the outcome of a prognostic test described herein, a clinician or physician may target pertinent information and preventative or therapeutic treatments to a subject who would be benefited by the information or treatment and avoid directing such information and treatments to a subject who would not be benefited (e.g., the treatment has no therapeutic effect and/or the subject experiences adverse side effects).
  • a particular treatment regimen can exert a differential effect depending upon the subject's genotype.
  • a candidate therapeutic exhibits a significant interaction with a major allele and a comparatively weak interaction with a minor allele (e.g., an order of magnitude or greater difference in the interaction)
  • such a therapeutic typically would not be administered to a subject genotyped as being homozygous for the minor allele, and sometimes not administered to a subject genotyped as being heterozygous for the minor allele.
  • a candidate therapeutic is not significantly toxic when administered to subjects who are homozygous for a major allele but ia comparatively toxic when administered to subjects heterozygous or homozygous for a minor allele
  • the candidate therapeutic is not typically administered to subjects who are genotyped as being heterozygous or homozygous with respect to the minor allele.
  • the methods described herein are applicable to pharmacogenomic methods for preventing, alleviating or treating type II diabetes conditions such as metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia.
  • type II diabetes conditions such as metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia.
  • a nucleic acid sample from an individual may be subjected to a prognostic test described herein.
  • information for preventing or treating type II diabetes and/or one or more type II diabetes treatment regimens then may be prescribed to that subject.
  • a treatment or preventative regimen is specifically prescribed and/or administered to individuals, who will most benefit from it based upon their risk of developing type II diabetes assessed by the methods described herein.
  • a treatment or preventative regimen is specifically prescribed and/or administered to individuals, who will most benefit from it based upon their risk of developing type II diabetes assessed by the methods described herein.
  • certain embodiments are directed to a method for reducing type II diabetes in a subject, which comprises: detecting the presence or absence of a polymorphic variant associated with type II diabetes in a nucleotide sequence in a nucleic acid sample from a subject, where the nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO: 1-20; (b)- a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; and (d) a fragment of a polynucle
  • Certain preventative treatments often are prescribed to subjects having a predisposition to type II diabetes and where the subject is diagnosed with type II diabetes or is diagnosed as having symptoms indicative of early stage type II diabetes, ⁇ e.g., impaired glucose tolerance, or IGT).
  • IGT impaired glucose tolerance
  • the treatment sometimes is preventative (e.g., is prescribed or administered to reduce the probability that a type II diabetes associated condition arises or progresses);, sometimes is therapeutic, and sometimes delays, alleviates or halts the progression of a type II diabetes associated condition. Any known preventative or therapeutic treatment for alleviating or preventing the occurrence of a type II diabetes associated disorder is prescribed and/or administered.
  • the treatment sometimes includes changes in diet, increased exercise, and the administration of therapeutics such as sulphonylureas (and related insulin secretagogues), which increase insulin release from pancreatic islets; metformin (GlucophageTM), which acts to reduce hepatic glucose production; peroxisome proliferator-activated receptor-gamma (PPAR) agonists (thiozolidinediones such as Avandia® and Actos®), which enhance insulin action; alpha- glucosidase inhibitors (e.g., Precose®, Voglibose®, and Miglitol®), which interfere with gut glucose absorption; and insulin itself, which suppresses glucose production and augments glucose utilization (Moller Nature 414, 821-827 (2001)).
  • therapeutics such as sulphonylureas (and related insulin secretagogues), which increase insulin release from pancreatic islets; metformin (GlucophageTM), which acts to reduce hepatic glucose production;
  • type II diabetes preventative and treatment information can be specifically targeted to subjects in need thereof (e.g., those at risk of developing type II diabetes or those that have early stages of type II diabetes), provided herein is a method for preventing or reducing the risk of developing type II diabetes in a subject, which comprises: (a) detecting the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying a subject with a predisposition to type II diabetes, whereby the presence of the polymorphic variation is indicative of a predisposition to type II diabetes in the subject; and (c) if such a predisposition is identified, providing the subject with information about methods or products to prevent or reduce type II diabetes or to delay the onset of type II diabetes.
  • Also provided is a method of targeting information or advertising to a subpopulation of a human population based on the subpopulation being genetically predisposed to a disease or condition which comprises: (a) detecting the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying the subpopulation of subjects in which the polymorphic variation is associated with type II diabetes; and (c) providing information only to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition.
  • Pharmacogenomics methods also may be used to analyze and predict a response to a type II diabetes treatment or a drug. For example, if pharmacogenomics analysis indicates a likelihood that an individual will respond positively to a type II diabetes treatment with a particular drug, the drug may be administered to the individual. Conversely, if the analysis indicates that an individual is likely to respond negatively to treatment with a particular drug, an alternative course of treatment may be prescribed. A negative response may be defined as either the absence of an efficacious response or the presence of toxic side effects.
  • the response to a therapeutic treatment can be predicted in a background study in which subjects in any of the following populations are genotyped: a population that responds favorably to a treatment regimen, a population that does not respond significantly to a treatment regimen, and a population that responds adversely to a treatment regiment (e.g., exhibits one or more side effects). These populations are provided as examples and other populations and subpopulations may be analyzed. Based upon the results of these analyses, a subject is genotyped to predict whether he or she will respond favorably to a treatment regimen, not respond significantly to a treatment regimen, or respond adversely to a treatment regimen. In an embodiment, a response to metformin is predicted based upon the genotype of a nucleic acid sample from a subject.
  • detecting the presence of a cytosine at a position corresponding to position 50104 in SEQ ID NO: 1 in a nucleic acid sample is predictive of the subject not being responsive to metformin, and the drug typically is not prescribed to a subject having such a genotype.
  • the tests described herein also are applicable to clinical drug trials.
  • One or more polymorphic variants indicative of response to an agent for treating type II diabetes or to side effects to an agent for treating type II diabetes may be identified using the methods described herein. Thereafter, potential participants in clinical trials of such an agent may be screened to identify those individuals most likely to respond favorably to the drug and exclude those likely to experience side effects. In that way, the effectiveness of drug treatment may be measured in individuals who respond positively to the drug, without lowering the measurement as a result of the inclusion of individuals who are unlikely to respond positively in the study and without risking undesirable safety problems.
  • another embodiment is a method of selecting an individual for inclusion in a clinical trial of a treatment or drug comprising the steps of: (a) obtaining a nucleic acid sample from an individual; (b) determining the identity of a polymorphic variation which is associated with a positive response to the treatment or the drug, or at least one polymorphic variation which is associated with a negative response to the treatment or the drug in the nucleic acid sample, and (c) including the individual in the clinical trial if the nucleic acid sample contains said polymorphic variation associated with a positive response to the treatment or the drug or if the nucleic acid sample lacks said polymorphic variation associated with a negative response to the treatment or the drug.
  • the polymorphic variation may be in a sequence selected individually or in any combination from the group consisting of (i) a nucleotide sequence of SEQ ID NO: 1-20; (ii) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (iii) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; and (iv) a fragment of a polynucleotide sequence of (i), (ii), or (iii) comprising of a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; and (iv) a fragment of a poly
  • step (c) optionally comprises- administering the drug or the treatment to the individual if the nucleic acid sample contains the polymorphic variation associated with a positive response to the treatment or the drug and the nucleic acid sample lacks said biallelic marker associated with a negative response to the treatment or the drug.
  • a method of partnering between a diagnostic/prognostic testing provider and a provider of a consumable product comprises: (a) the diagnostic/prognostic testing provider detects the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) the diagnostic/prognostic testing provider identifies the subpopulation of subjects in which the polymorphic variation is associated with type II diabetes; (c) the diagnostic/prognostic testing provider forwards information to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition; and (d) the provider of a consumable product forwards to the diagnostic test provider a fee every time the diagnostic/prognostic test provider forwards information to the subject as set forth in step (c) above.
  • composition comprising a cell from a subject having type II diabetes or at risk of type II diabetes and one or more molecules specifically directed and targeted to a nucleic acid comprising a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence or amino acid sequence.
  • Such directed molecules include, but are not limited to, a compound that binds to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence or amino acid sequence referenced herein; a nucleic acid that hybridizes to a SLC22A 1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid under conditions of high stringency; a RNAi or siRNA molecule having a strand complementary to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence; an antisense nucleic acid complementary to an RNA encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence
  • the diabetes directed molecule interacts with a nucleic acid or polypeptide variant associated with diabetes, such as variants referenced herein.
  • the diabetes directed molecule interacts with a polypeptide involved in a signal pathway of a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, FRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a nucleic acid comprising such a nucleotide sequence.
  • compositions sometimea include an adjuvant known to stimulate an immune response, and in certain embodiments, an adjuvant that stimulates a T-cell lymphocyte response.
  • Adjuvants are known, including but not limited to an aluminum adjuvant (e.g., aluminum hydroxide); a cytokine adjuvant or adjuvant that stimulates a cytokine response (e.g., interleukin (IL)-12 and/or ⁇ - interferon cytokines); a Freund-type mineral oil adjuvant emulsion (e.g., Freund's complete or incomplete adjuvant); a synthetic lipoid compound; a copolymer adjuvant (e.g., TitreMax); a saponin; Quil A; a liposome; an oil-in-water emulsion (e.g., an emulsion stabilized by Tween SO and pluronic polyoxyethlene/polyoxypropylene block copolymer (Syntex Adjuvant Formulation
  • compositions are useful for generating an immune response against a diabetes directed molecule (e.g., an HLA- binding subsequence within a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence).
  • a diabetes directed molecule e.g., an HLA- binding subsequence within a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence.
  • a peptide having an amino acid subsequence of a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence is delivered to a subject, where the subsequence binds to an HLA molecule and induces a CTL lymphocyte response.
  • the peptide sometimes is delivered to the subject as an isolated peptide or as a minigene in a plasmid that encodes the peptide.
  • the cell may be in a group of cells cultured in vitro or in a tissue maintained in vitro or present in an animal in vivo (e.g., a rat, mouse, ape or human).
  • a composition comprises a component from a cell such as a nucleic acid molecule (e.g., genomic DNA), a protein mixture or isolated protein, for example.
  • a nucleic acid molecule e.g., genomic DNA
  • the aforementioned compositions have utility in diagnostic, prognostic and pharmacogenomic methods described previously and in diabetes therapeutics described hereafter. Certain diabetes directed molecules are described in greater detail below.
  • Compounds can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; peptoid libraries (libraries of molecules having the functionalities of peptides, but with a novel, non-peptide backbone which are resistant to enzymatic degradation but which nevertheless remain bioactive (see, e.g., Zuckermann et al, J. Med. Chem.37: 2678-85 (1994)); spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; "one-bead one-compound” library methods; and synthetic library methods using affinity chromatography selection.
  • Biolibrary and peptoid library approaches are typically limited to peptide libraries, while the other approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds (Lam, Anticancer Drug Des. 12: 145, (1997)).
  • Examples of methods for synthesizing molecular libraries are described, for example, in DeWitt et al, Proc. Natl. Acad. Sci. U.S.A. 90: 6909 (1993); Erb et al, Proc. Natl. Acad. Sci. USA 91: 11422 (1994); Zuckermann et al, J. Med. Chem.
  • a compound sometimes alters expression and sometimes alters activity of a polypeptide target and may be a small molecule.
  • Small molecules include, but are not limited to, peptides, peptidomimetics ⁇ e.g., peptoids), amino acids, amino acid analogs, polynucleotides, polynucleotide analogs, nucleotides, nucleotide analogs, organic or inorganic compounds ⁇ i.e., including heteroorganic and organometallic compounds) having, a molecular weight less than about 10,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 5,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 1,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 500 grams per mole, and salts, esters, and other pharmaceutically acceptable forms of such compounds.
  • an "antisense” nucleic acid refers to a nucleotide sequence complementary to a "sense" nucleic acid encoding a polypeptide, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mKNA sequence.
  • the antisense nucleic acid can be complementary to an entire coding strand ⁇ e.g., SEQ ID NO: 8-16 ⁇ , or to a portion thereof or a substantially identical sequence thereof.
  • the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence ⁇ e.g., 5' and 3' untranslated regions in SEQ ID NO: 1-9).
  • An antisense nucleic acid can be designed such that it is complementary to the entire coding region of an mRNA encoded by a nucleotide sequence ⁇ e.g., SEQ ID NO: 1-20), and often the antisense nucleic acid is an oligonucleotide antisense to only a portion of a coding or noncoding region of the mRNA.
  • the antisense oligonucleotide can be complementary to the region surrounding the translation start site of the mRNA, e.g., between the -10 and +10 regions of the target gene nucleotide sequence of interest.
  • An antisense oligonucleotide can be, for example, about 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or more nucleotides in length.
  • the antisense nucleic acids which include the ribozymes described hereafter, can be designed to target a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequence, often a variant associated with diabetes, or a substantially identical sequence thereof.
  • minor alleles and major alleles can be targeted, and those associated with a higher risk of diabetes are often designed, tested, and administered to subjects.
  • An antisense nucleic acid can be constructed using chemical synthesis and enzymatic ligation reactions using standard procedures.
  • an antisense nucleic acid ⁇ e.g., an antisense oligonucleotide
  • an antisense nucleic acid can be chemically synthesized using naturally occurring nucleotides, or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used.
  • Antisense nucleic acid also can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation ⁇ i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).
  • antisense nucleic acids When utilized as therapeutics, antisense nucleic acids typically are administered to a subject ⁇ e.g., by direct injection at a tissue site) or generated in situ such that they hybridize with or bind to cellular niRNA and/or genomic DNA encoding a polypeptide and thereby inhibit expression of the polypeptide, for example, by inhibiting transcription and/or translation.
  • antisense nucleic acid molecules can be modified to target selected cells and then are administered systemically.
  • antisense molecules can be modified such that they specifically bind to receptors, or antigens expressed on a selected cell surface, for example, by linking antisense nucleic acid molecules to peptides or antibodies which bind to cell surface receptors or antigens.
  • Antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. Sufficient intracellular concentrations of antisense molecules, are achieved by incorporating a strong promoter, such as a pol II or pol III promoter, in the vector construct.
  • a strong promoter such as a pol II or pol III promoter
  • Antisense nucleic acid molecules sometimes, are *-anomeric nucleic acid molecules.
  • An *-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual *-units, the strands run parallel to each other (Gaultier et ah, Nucleic Acids. Res. 15: 6625-6641 (1987)).
  • Antisense nucleic acid molecules can also comprise a 2'-o-methylribonucleotide (Inoue et ah, Nucleic Acids Res. 15: 6131-6148 (1987)) or a chimeric RNA-DNA analogue (Inoue et ah, FEBS Lett.
  • an antisense nucleic acid is a ribozyme.
  • a ribozyme having specificity for a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence can include one or more sequences complementary to such a nucleotide sequence, and a sequence having a known catalytic region responsible for mRNA cleavage (see e.g., U.S. Pat. No.
  • a derivative of a Tetrahymena L- 19 IVS RNA is sometimes utilized in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a mRNA (see e.g., Cech et al. U.S. Patent No. 4,987,071; and Cech et al. U.S. Patent No. 5,116,742).
  • target mRNA sequences can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules (see e.g., Bartel & Szostak, Science 261: 1411-1418 (1993)).
  • Diabetes directed molecules include in certain embodiments nucleic acids that can form triple helix structures with a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence or a substantially identical sequence thereof, especially one that includes a regulatory region that controls expression of a polypeptide.
  • Gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of a nucleotide sequence referenced herein or a substantially identical sequence (e.g., promoter and/or enhancers) to form triple helical structures that prevent transcription of a gene in target cells (see e.g., Helene, Anticancer Drug Des.
  • Switchback molecules are synthesized in an alternating 5'-3', 3'-5' manner, such that they base pair with first one strand of a duplex and then the other, eliminating the necessity for a sizeable stretch of either purines or pyrimidines to be present on one strand of a duplex.
  • Diabetes directed molecules include RNAi and siRNA nucleic acids. Gene expression may be inhibited by the introduction of double-stranded RNA (dsRNA), which induces potent and specific gene silencing, a phenomenon called RNA interference or RNAi.
  • dsRNA double-stranded RNA
  • RNAi RNA interference
  • Fire et al US Patent Number 6,506,559
  • Tuschl et al. PCT International Publication No. WO 01/75164
  • Bosher JM Labouesse, Nat Cell Biol 2000 Feb;2(2):E31-6.
  • RNA interference RNA interference
  • siRNA refers to a nucleic acid that forms a double stranded RNA and has the ability to reduce or inhibit expression of a gene or target gene when the siRNA is delivered to or expressed in the same cell as the gene or target gene.
  • siRNA refers to short double-stranded RNA formed by the complementary strands. Complementary portions of the siRNA that hybridize to form the double stranded molecule often have substantial or complete identity to the target molecule sequence.
  • an siRNA refers to a nucleic acid that has substantial or complete identity to a target gene and forms a double stranded siRNA.
  • the targeted region When designing the siRNA molecules, the targeted region often is selected from a given DNA sequence beginning 50 to 100 nucleotides downstream of the start codon. See, e.g., Elbashir et al,. Methods 26:199-213 (2002). Initially, 5' or 3' UTRs and regions nearby the start codon were avoided assuming that UTR-binding proteins and/or translation initiation complexes may interfere with binding of the siRNP or RISC endonuclease complex. Sometimes regions of the target 23 nucleotides in length conforming to the sequence motif AA(Nl 9)TT (N, an nucleotideX and regions with approximately 30% to 70% G/C-content (often about 50% G/C-content) often are selected.
  • the sequence of the sense siRNA sometimes corresponds to (N 19) TT or N21 (position 3 to 23 of the 23 -nt motif), respectively. In the latter case, the 3' end of the sense siRNA often is converted to TT.
  • the rationale for this sequence conversion is to generate a symmetric duplex with respect to the sequence composition of the sense and antisense 3' overhangs.
  • the antisense siRNA is synthesized as the complement to position 1 to 21 of the 23 -nt motif. Because position 1 of the 23- nt motif is not recognized sequence-specifically by the antisense siRNA, the 3 '-most nucleotide residue of the antisense siRNA can be chosen deliberately.
  • the penultimate nucleotide of the antisense siRNA (complementary to position 2 of the 23 -nt motif) often is complementary to the targeted sequence.
  • TT often is utilized.
  • Respective 21 nucleotide sense and antisense siRNAs often begin with a purine nucleotide and can also be expressed from pol III expression vectors without a change in targeting site. Expression of RNAs from pol III promoters often is efficient when the first transcribed nucleotide is a purine.
  • the sequence of the siRNA can correspond to the full length target gene, or a subsequence thereof.
  • the siRNA is about 15 to about 50 nucleotides in length (e.g., each complementary sequence of the double stranded siRNA is 15-50 nucleotides in length, and the double stranded siRNA is about 15-50 base pairs in length, sometimes about 20-30 nucleotides in length or about 20-25 nucleotides in length, e.g., 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length.
  • the siRNA sometimes is about 21 nucleotides in length.
  • siRNA molecules sometimes is of a different chemical composition as compared to native RNA that imparts increased stability in cells (e.g., decreased susceptibility to degradation), and sometimes includes one or more modifications in siSTABLE RNA described at the http address www.dharmacon.com.
  • Antisense, ribozyme, RNAi and siRNA nucleic acids can be altered to form modified nucleic acid molecules.
  • the nucleic acids can be altered at base moieties, sugar moieties or phosphate backbone moieties to improve stability, hybridization, or solubility of the molecule.
  • the deoxyribose phosphate backbone of nucleic acid molecules can be modified to generate peptide nucleic acids (see Hyrup et ah, Bioorganic & Medicinal Chemistry 4 (1): 5-23 (1996)).
  • peptide nucleic acid refers to a nucleic acid mimic such as a DNA mimic, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained.
  • the neutral backbone of a PNA can allow for specific hybridization to DNA and RNA under conditions of low ionic strength. Synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described, for example, in Hyrup et ah, (1996) supra and Perry-O'Keefe et ah, Proc. Natl. Acad. Sci. 93: 14670-675 (1996).
  • PNA nucleic acids can be used in prognostic, diagnostic, and therapeutic applications.
  • PNAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, for example, inducing transcription or translation arrest or inhibiting replication.
  • PNA nucleic acid molecules can also be used in the analysis of single base pair mutations in a gene, (e.g., by PNA-directed PCR clamping); as "artificial restriction enzymes" when used in combination with other enzymes, (e.g., Sl nucleases (Hyrup (1996) supra)); or as probes or primers for DNA sequencing or hybridization (Hyrup et ah, (1996) supra; Perry-O'Keefe supra).
  • oligonucleotides may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across cell membranes (see e.g., Letsinger et al., Proc. Natl. Acad. Sci. USA 86: 6553-6556 (1989); Lemaitre et ah, Proc. Natl. Acad. Sci. USA 84: 648-652 (1987); PCT Publication No. W088/09810) or the blood-brain barrier (see, e.g., PCT Publication No. W089/10134).
  • peptides e.g., for targeting host cell receptors in vivo
  • agents facilitating transport across cell membranes see e.g., Letsinger et al., Proc. Natl. Acad. Sci. USA 86: 6553-6556 (1989); Lemaitre et ah, Proc. Natl. Acad. Sci
  • oligonucleotides can be modified with hybridization-triggered cleavage agents (See, e.g., Krol et al., Bio-Techniques 6: 958-976 (1988)) or intercalating agents. (See, e.g., Zon, Pharm, Res. 5: 539-549 (1988) ).
  • the oligonucleotide may be conjugated to another molecule, (e.g., a peptide, hybridization triggered cross-linking agent, transport agent, or hybridization-triggered cleavage agent).
  • molecular beacon oligonucleotide primer and probe molecules having one or more regions complementary to a SLC22A1, TTN, LOCI 12609, TKPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence or a substantially identical sequence thereof, two complementary regions one having a fluorophore and one a quencher such that the molecular beacon is useful for quantifying the presence of the nucleic acid in a sample.
  • Molecular beacon nucleic acids are described, for example, in Lizardi et ah, U.S. Patent No. 5,854,033; Nazarenko et al, U.S. Patent No. 5,866,336, and Livak et al, U.S. Patent 5,876,930.
  • antibody refers to an immunoglobulin molecule or immunologically active portion thereof, i.e., an antigen-binding portion.
  • immunologically active portions of immunoglobulin molecules include F(ab) and F(ab') 2 fragments which can be generated by treating the antibody with an enzyme such as pepsin.
  • An antibody sometimes is a polyclonal, monoclonal, recombinant ⁇ e.g., a chimeric or humanized), fully human, non-human (e.g., murine), or a single chain antibody.
  • An antibody may have effector function and can fix complement, and is sometimes coupled to a toxin or imaging agent.
  • a full-length polypeptide or antigenic peptide fragment encoded by a nucleotide sequence referenced herein can be uaed as an immunogen or can be used to identify antibodies made with other immunogens, e.g., cells, membrane preparations, and the like.
  • An antigenic peptide often includes at least 8 amino acid residues of the amino acid sequences encoded by a nucleotide sequence referenced herein, or substantially identical sequence thereof, and encompasses an epitope.
  • Antigenic peptides sometimes include 10 or more amino acids, 15 or more amino acids, 20 or more amino acids, or 30 or more amino acids. Hydrophilic and hydrophobic fragments of polypeptides sometimes are used as immunogens.
  • Epitopes encompassed by the antigenic peptide are regions located on the surface of the polypeptide (e.g., hydrophilic regions) as well as regions with high antigenicity.
  • regions located on the surface of the polypeptide e.g., hydrophilic regions
  • an Emini surface probability analysis of the human polypeptide sequence can be used to indicate the regions that have a particularly high probability of being localized to the surface of the polypeptide and are thus likely to constitute surface residues useful for targeting antibody production.
  • the antibody may bind an epitope on any domain or region on polypeptides described herein.
  • chimeric, humanized, and completely human antibodies are useful for applications which include repeated administration to subjects.
  • Chimeric and humanized monoclonal antibodies comprising both human and non-human portions, can be made using standard recombinant DNA techniques.
  • Such chimeric and humanized monoclonal antibodies can be produced by recombinant DNA techniques known in the art, for example using methods described in Robinson et al International Application No. PCT/US86/02269; Akira, et al European Patent Application 184,187; Taniguchi, M., European Patent Application 171,496; Morrison et al European Patent Application 173,494; Neuberger et al PCT International Publication No.
  • Completely human antibodies are particularly desirable for therapeutic treatment of human patients.
  • Such antibodies can be produced using transgenic mice that are incapable of expressing endogenous immunoglobulin heavy and light chains genes, but which can express human heavy and light chain genes. See, for example, Lonberg and Huszar, Int. Rev. Immunol. 13: 65-93 (1995); and U.S. Patent Nos. 5,625,126; 5,633,425; 5,569,825; 5,661,016; and 5,545,806.
  • companies such as Abgenix, Inc. (Fremont, CA) and Medarex, Inc. (Princeton, NJ ⁇ , can be engaged to provide human antibodies directed against a selected antigen using technology similar to that described above.
  • Completely human antibodies that recognize a selected epitope also can be generated using a technique referred to as "guided selection.”
  • a selected non- human monoclonal antibody ⁇ e.g., a murine antibody
  • This technology is described for example by Jespers et al, Bio/Technology 12: 899-903 (1994).
  • An antibody can be a single chain antibody.
  • a single chain antibody (scFV) can be engineered (see, e.g., Colcher et al, Ann. N Y Acad. Sci. 880: 263-80 (1999); and Reiter, Clin. Cancer Res. 2: 245-52 (1996)).
  • Single chain antibodies can be dimerized or multimerized to generate multivalent antibodies having specificities for different epitopes of the same target polypeptide.
  • Antibodies also may be selected or modified so that they exhibit reduced or no ability to bind an Fc receptor.
  • an antibody may be an isotype or subtype, fragment or other mutant, which does not support binding to an Fc receptor (e.g., it has a mutagenized or deleted Fc receptor binding region).
  • an antibody may be conjugated to a therapeutic moiety such as a cytotoxin, a therapeutic agent or a radioactive metal ion.
  • a cytotoxin or cytotoxic agent includes any agent that is detrimental to cells.
  • Examples include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1 dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof.
  • Therapeutic agents include, but are not limited to, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-ftuorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thiotepa chlorambucil, melphalan, carmustine (BCNU) and lomustine (CCNU), cyclophosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin ⁇ , bleomycin, mithramycin, and anthramycin (AMC)), and anti ⁇ mitotic agents (
  • Antibody conjugates can be used for modifying a given biological response.
  • the drug moiety may be a protein or polypeptide possessing a desired biological activity.
  • proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, ⁇ -interferon, ⁇ -interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator; or, biological response modifiers such as, for example, lymphokines, interleukin-1 ("IL-I”), interleukin-2 (“IL-2”), interleukin-6 (“IL-6”), granulocyte macrophage colony stimulating factor (“GM-CSF”), granulocyte colony stimulating factor (“G-CSF”), or other growth factors.
  • an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Patent No. 4,676,
  • An antibody e.g., monoclonal antibody
  • an antibody can be used to isolate target polypeptides by standard techniques, such as affinity chromatography or immunoprecipitation.
  • an antibody can be used to detect a target polypeptide (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the polypeptide.
  • Antibodies can be used diagnostically to monitor polypeptide levels in tissue as part of a clinical testing procedure, e.g., to determine the efficacy of a given treatment regimen. Detection can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance (i.e., antibody labeling).
  • detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials.
  • suitable enzymes include horseradish peroxidase, alkaline phosphatase, ⁇ -galactosidase, or acetylcholinesterase;
  • suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin;
  • suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin;
  • an example of a luminescent material includes luminol;
  • bioluminescent materials include luciferase, luciferin, and aequorin, and
  • suitable radioactive material include 125 1, 131 1, 35 S or 3 H.
  • an antibody can be utilized as a test molecule for determining whether it can treat diabetes, and
  • An antibody can be made by immunizing with a purified antigen, or a fragment thereof, e.g., a fragment described herein, a membrane associated antigen, tissues, e.g., crude tissue preparations, whole cells, preferably living cells, lysed cells, or cell fractions.
  • a purified antigen or a fragment thereof, e.g., a fragment described herein, a membrane associated antigen, tissues, e.g., crude tissue preparations, whole cells, preferably living cells, lysed cells, or cell fractions.
  • the methods comprise contacting a test molecule with a target molecule in a system.
  • a "target molecule” as used herein refers to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid, a substantially identical nucleic acid thereof, or a fragment thereof, and an encoded polypeptide of the foregoing.
  • the methods also comprise determining the presence or absence of an interaction between the test molecule and the target molecule, where the presence of an interaction between the test molecule and the nucleic acid or polypeptide identifies the test molecule as a candidate type II diabetes therapeutic.
  • the interaction between the test molecule and the target molecule may be quantified.
  • Test molecules and candidate therapeutics include, but are not limited to, compounds, antisense nucleic acids, siRNA molecules, ribozymes, polypeptides or proteins encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence, or a substantially identical sequence or fragment thereof, and immunotherapeutics. (e.g., antibodies and HLA-presented polypeptide fragments).
  • a test molecule or candidate therapeutic may act as a modulator of target molecule concentration or target molecule function in a system.
  • a “modulator” may agonize ⁇ i.e., up-regulates) or antagonize (i.e., down-regulates) a target molecule concentration partially or completely in a system by affecting such cellular functions as DNA replication and/or DNA processing ⁇ e.g., DNA methylation or DNA repair), RNA transcription and/or RNA processing ⁇ e.g., removal of intronic sequences and/or translocation of spliced mRNA from the nucleus), polypeptide production (e.g., translation of the polypeptide from mRNA), and/or polypeptide post-translational modification (e.g., glycosylation, phosphorylation, and proteolysis, of pro-polypeptides).
  • DNA replication and/or DNA processing e.g., DNA methylation or DNA repair
  • RNA transcription and/or RNA processing e.g., removal of intronic sequences and/or translocation of spliced mRNA from the nucleus
  • a modulator may also agonize or antagonize a biological function of a target molecule partially or completely, where the function may include adopting a certain structural conformation, interacting with one or more binding partners, ligand binding, catalysis (e.g., phosphorylation, dephosphorylation, hydrolysis, methylation, and isomerization), and an effect upon a cellular event (e.g., effecting progression of type II diabetes).
  • a candidate therapeutic increases glucose uptake in cells of a subject (e.g., in certain cells of the pancreas).
  • system refers to a cell free in vitro environment and a cell- based environment such as a collection of cells, a tissue, an organ, or an organism.
  • a system is "contacted” with a test molecule in a variety of manners, including adding molecules in solution and allowing them to interact with one another by diffusion, cell injection, and any administration routes in an animal.
  • interaction refers to an effect of a test molecule on test molecule, where the effect sometimes is binding between the test molecule and the target molecule, and sometimes is an observable change in cells, tissue, or organism.
  • Test molecule/target molecule interactions can be detected and/or quantified using assays known in the art. For example, an interaction can be determined by labeling the test molecule and/or the target molecule, where the label is covalently or non-covaleiitly attached to the test molecule or target molecule.
  • the label is sometimes a radioactive molecule such as 125 1, 131 I, 35 S or ⁇ , which can be detected by direct counting of radioemission or by scintillation counting.
  • enzymatic labels such as horseradish peroxidase, alkaline phosphatase, or luciferase may be utilized where the enzymatic label can be detected by determining conversion of an appropriate substrate to product.
  • a microphysiometer e.g., Cytosensor
  • LAPS light-addressable potentiometric sensor
  • cells typically include a SLC22A1, TTN, LOC112609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid, an encoded polypeptide, or substantially identical nucleic acid or polypeptide thereof, and are often of mammalian origin, although the cell can be of any origin.
  • Whole cells, cell homogenates, and cell fractions e.g., cell membrane fractions
  • soluble and/or membrane bound forms of the polypeptide may be utilized.
  • membrane-bound forms of the polypeptide it may be desirable to utilize a solubilizing agent.
  • solubilizing agents include non-ionic detergents such as n- octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide, decanoyl-
  • An interaction between a test molecule and target molecule also can be detected by monitoring fluorescence energy transfer (FET) (see, e.g., Lakowicz et at, U.S. Patent No. 5,631,169; Stavrianopoulos et al. U.S. Patent No. 4,868,103).
  • FET fluorescence energy transfer
  • a fluorophore label on a first, "donor” molecule is selected such that its emitted fluorescent energy will be absorbed by a fluorescent label on a second, "acceptor” molecule, which in turn is able to fluoresce due to the absorbed energy.
  • the "donor" polypeptide molecule may simply utilize the natural fluorescent energy of tryptophan residues.
  • Labels are chosen that emit different wavelengths of light, such that the "acceptor” molecule label may be differentiated from that of the "donor". Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, the spatial relationship between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the "acceptor" molecule label in the assay should be maximal.
  • An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art ⁇ e.g., using a fluorimeter).
  • determining the presence or absence of an interaction between a test molecule and a target molecule can be effected by monitoring surface plasmon resonance ⁇ see, e.g., Sjolander & Urbaniczk, Anal. Chem. 63: 2338-2345 (1991) and Szabo et al, Curr. Opin. Struct. Biol. 5: 699-705 (1995)).
  • surface plasmon resonance or “biomolecular interaction analysis (BIA)” can be utilized to detect biospecific interactions in real time, without labeling any of the interactants ⁇ e.g., BIAcore ⁇ .
  • the target molecule or test molecules are anchored to a solid phase, facilitating the detection of target molecule/test molecule complexes and separation of the complexes from free, uncomplexed molecules.
  • the target molecule or test molecule is immobilized to the solid support.
  • the target molecule is anchored to a solid surface, and the test molecule, which is not anchored, can be labeled, either directly or indirectly, with detectable labels discussed herein.
  • test molecules may be desirable to immobilize a target molecule, an anti-target molecule antibody, and/or test molecules to facilitate separation of target molecule/test molecule complexes from uncomplexed forms, as well as to accommodate automation of the assay.
  • the attachment between a test molecule and/or target molecule and the solid support may be covalent or non-covalent ⁇ see, e.g., U.S. Patent No. 6,022,688 for non-covalent attachments).
  • the solid support may be one or more surfaces of the system, such as one or more surfaces in each well of a microtiter plate, a surface of a silicon wafer, a surface of a bead ⁇ see, e.g., Lam, Nature 354: 82-84 (1991))- that is optionally linked to another solid support, or a channel in a microfluidic device, for example.
  • Types of solid supports, linker molecules for covalent and non-covalent attachments to solid supports, and methods for immobilizing nucleic acids and other molecules to solid supports are well known ⁇ see, e.g., U.S. Patent Nos. 6,261,776; 5,900,481; 6,133,436; and 6,022,688; and WIPO publication WO 01/18234).
  • target molecule may be immobilized to surfaces via biotin and streptavidin.
  • biotinylated target polypeptide can be prepared from biotin-NHS (N- hydroxy-succinimide) using techniques known in the art ⁇ e.g., biotinylation kit, Pierce Chemicals, Rockford, IL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical).
  • a target polypeptide can be prepared as a fusion polypeptide.
  • glutathione-S-transferase/target polypeptide fusion can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St.
  • the beads or microtiter plate wells are washed to remove any unbound components, or the matrix is immobilized in the case of beads, and complex formation is determined directly or indirectly as described above.
  • the complexes can be dissociated from the matrix, and the level of target molecule binding or activity is determined using standard techniques.
  • the non-immobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that a significant percentage of complexes formed will remain immobilized to the solid surface.
  • the detection of complexes anchored on the solid surface can be accomplished in a number of manners. Where the previously non-immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed.
  • an indirect label can be used to detect complexes anchored on the surface, e.g., by adding a labeled antibody specific for the immobilized component, where the antibody, in turn, can be directly labeled or indirectly labeled with, e.g., a labeled anti-Ig antibody.
  • an assay is performed utilizing antibodies that specifically bind target molecule or test molecule but do not interfere with binding of the target molecule to the test molecule.
  • Such antibodies can be derivitized to a solid support, and unbound target molecule may be immobilized by antibody conjugation.
  • Methods for detecting such complexes include immunodetection of complexes using antibodies reactive with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.
  • Cell free assays also can be conducted in a liquid phase.
  • reaction products are separated from unreacted components, by any of a number of standard techniques, including but not limited to: differential centrifugation (see, e.g., Rivas, G., and Minton, Trends Biochem SciAug;18(8): 284-7 (1993)); chromatography (gel filtration chromatography, ion- exchange chromatography); electrophoresis (see, e.g., Ausubel et al., eds. Current Protocols in Molecular Biology , J.
  • a cell or cell free mixture is contacted with a candidate compound and the expression of target mRNA or target polypeptide is evaluated relative to the level of expression of target mRNA or target polypeptide in the absence of the candidate compound.
  • the candidate compound is identified as an agonist of target mRNA or target polypeptide expression.
  • the candidate compound is. identified as an antagonist or inhibitor of target mRNA or target polypeptide expression.
  • the level of target mRNA or target polypeptide expression can be determined by methods described herein.
  • binding partners that interact with a target molecule are detected.
  • the target molecules can interact with one or more cellular or extracellular macromolecules, such as polypeptides in vivo, and these interacting molecules are referred to herein as "binding partners.”
  • Binding partners can agonize or antagonize target molecule biological activity.
  • test molecules that agonize or antagonize interactions between target molecules and binding partners can be useful as therapeutic molecules as they can up-regulate or down-regulated target molecule activity in vivo and thereby treat type II diabetes.
  • Binding partners of target molecules can be identified by methods, known in the art. For example, binding partners may be identified by lysing cells and analyzing cell lysates by electrophoretic techniques. Alternatively, a two-hybrid assay or three-hybrid assay can be utilized (see, e.g., U.S. Patent No. 5,283,317; Zervos et al., Cell 72:223-232 (1993); Madura et al, J. Biol. Chem.
  • a two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. The assay often utilizes two different DNA constructs.
  • a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid (sometimes referred to as the "bait") is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4).
  • a DNA sequence from a library of DNA sequences that encodes a potential binding partner (sometimes referred to as the "prey") is fused to a gene that encodes an activation domain of the known transcription factor.
  • a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid can be fused to the activation domain. If the "bait" and the “prey” molecules interact in vivo, the DNA- binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) which is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to identify the potential binding partner.
  • a reporter gene e.g., LacZ
  • a reaction mixture containing the target molecule and the binding partner is prepared, under conditions and for a time sufficient to allow complex formation.
  • the reaction mixture often is provided in the presence or absence of the test molecule.
  • the test molecule can be included initially in the reaction mixture, or can be added at a time subsequent to the addition of the target molecule and its binding partner. Control reaction mixtures are incubated without the test molecule or with a placebo. Formation of any complexes between the target molecule and the binding partner then is detected.
  • Decreased formation of a complex in the reaction mixture containing test molecule as compared to in a control reaction mixture indicates that the molecule antagonizes target molecule/binding partner complex formation.
  • increased formation of a complex in the reaction mixture containing test molecule as. compared to in a control reaction mixture indicates that the molecule agonizes target molecule/binding partner complex formation.
  • complex formation of target molecule/binding partner can be compared to complex formation of mutant target molecule/binding partner (e.g., amino acid modifications in a target polypeptide). Such a comparison can be important in those cases where it is desirable to identify test molecules that modulate interactions of mutant but not non-mutated target gene products.
  • the assays can be conducted in a heterogeneous or homogeneous format.
  • target molecule and/or the binding partner are immobilized to a solid phase, and complexes are detected on the solid phase at the end of the reaction.
  • homogeneous assays the entire reaction is carried out in a liquid phase.
  • the order of addition of reactants can be varied to obtain different information about the molecules being tested.
  • test compounds that agonize target molecule/binding partner interactions can be identified by conducting the reaction in the presence of the test molecule in a competition format.
  • test molecules that agonize preformed complexes e.g., molecules with higher binding constants that displace one of the components from the complex, can be tested by adding the test compound to the reaction mixture after complexes have been formed.
  • the target molecule or the binding partner is anchored onto a solid surface (e.g., a microtiter plate), while the non-anchored species is labeled, either directly or indirectly.
  • the anchored molecule can be immobilized by non-covalent or covalent attachments.
  • an immobilized antibody specific for the molecule to be anchored can be used to anchor the molecule to the solid surface.
  • the partner of the immobilized species is exposed to the coated surface with or without the test molecule. After the reaction is complete, unreacted components are removed (e.g., by washing) such that a significant portion of any complexes formed will remain immobilized on the solid surface.
  • the detection of label immobilized on the surface is indicative of complex.
  • an indirect label can be used to detect complexes anchored to the surface; e.g., by using a labeled antibody specific for the initially non- immobilized species.
  • test compounds that inhibit complex formation or that disrupt preformed complexes can be detected.
  • the reaction can be conducted in a liquid phase in the presence or absence of test molecule, where the reaction products are separated from unreacted components, and the complexes are detected (e.g., using an immobilized antibody specific for one of the binding components to anchor any complexes formed in solution, and a labeled antibody specific for the other partner to detect anchored complexes)-.
  • test compounds that inhibit complex or that disrupt preformed complexes can be identified.
  • a homogeneous assay can be utilized. For example, a preformed complex of the target gene product and the interactive cellular or extracellular binding partner product is prepared. One or both of the target molecule or binding partner is labeled, and the signal generated by the label(s) is quenched upon complex formation (e.g., U.S. Patent No. 4,109,496 that utilizes this approach for immunoassays). Addition of a test molecule that competes with and displaces one of the species from the preformed complex will result in the generation of a signal above background. In this way, test substances that disrupt target molecule/binding partner complexes can be identified.
  • Candidate therapeutics for treating type II diabetes are identified from a group of test molecules that interact with a target molecule.
  • Test molecules are normally ranked according to the degree with which they modulate (e.g., agonize or antagonize) a function associated with the target molecule (e.g., DNA replication and/or processing, RNA transcription and/or processing, polypeptide production and/or processing, and/or biological function/activity), and then top ranking modulators are selected.
  • pharmacogenomic information described herein can determine the rank of a modulator. The top 10% of ranked test molecules often are selected for further testing as candidate therapeutics, and sometimes the top 15%, 20%, or 25% of ranked test molecules are selected for further testing as candidate therapeutics.
  • Candidate therapeutics typically are formulated for administration to a subject. Therapeutic Formulations
  • Formulations and pharmaceutical compositions typically include in combination with a pharmaceutically acceptable carrier one or more target molecule modulators.
  • the modulator often is a test molecule identified as having an interaction with a target molecule by a screening method described above.
  • the modulator may be a compound, an antisense nucleic acid, a ribozyme, an antibody, or a binding partner.
  • formulations may comprise a target polypeptide or fragment thereof in combination with a pharmaceutically acceptable carrier
  • the term "pharmaceutically acceptable carrier” includes solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Supplementary active compounds can also be incorporated into the compositions. Pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration.
  • a pharmaceutical composition typically is formulated to be compatible with its intended route of administration.
  • routes of administration include parenteral, e.g. , intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, and rectal administration.
  • Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide.
  • the parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
  • Oral compositions generally include an inert diluent or an edible carrier.
  • the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules, e.g., gelatin capsules.
  • Oral compositions can also be prepared using a fluid carrier for use as a mouthwash.
  • Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition.
  • the tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.
  • a binder such as microcrystalline cellulose, gum tragacanth or gelatin
  • an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch
  • a lubricant such as magnesium stearate or Sterotes
  • a glidant such as colloidal silicon dioxide
  • compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion.
  • suitable carriers include physiological saline, bacteriostatic water, Cremophor ELTM (BASF, Parsippany, NJ) or phosphate buffered saline (PBS).
  • the composition must be sterile and should be fluid to the extent that easy syringability exists. It should be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
  • the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof.
  • the proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like, hi many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition.
  • isotonic agents for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition.
  • Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.
  • Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above.
  • the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
  • a suitable propellant e.g., a gas such as carbon dioxide, or a nebulizer.
  • Systemic administration can also be by transmucosal or transdermal means.
  • penetrants appropriate to the barrier to be permeated are used in the formulation.
  • penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives.
  • Transmucosal administration can be accomplished through the use of nasal sprays or suppositories.
  • the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.
  • Molecules can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
  • active molecules are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems.
  • a controlled release formulation including implants and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. Materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811.
  • Dosage unit form refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED 50 (the dose therapeutically effective in 50% of the population).
  • the dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD 50 /ED 50 .
  • Molecules which exhibit high therapeutic indices are preferred. While molecules that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.
  • the data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans.
  • the dosage of such molecules lies preferably within a range of circulating concentrations that include the ED 50 with little or no toxicity.
  • the dosage may vary within this range depending upon the dosage form employed and the route of administration utilized.
  • the therapeutically effective dose can be estimated initially from cell culture assays.
  • a dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC 50 (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture.
  • IC 50 i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms
  • levels in plasma may be measured, for example, by high performance liquid chromatography.
  • a therapeutically effective amount of protein or polypeptide ranges from about 0.001 to 30 mg/kg body weight, sometimes about 0.01 to 25 mg/kg body weight, often about 0.1 to 20 mg/kg body weight, and more often about 1 to 10 mg/kg, 2 to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight.
  • the protein or polypeptide can be administered one time per week for between about 1 to 10 weeks, sometimes between 2 to 8 weeks, often between about 3 to 7 weeks, and more often for about 4, 5, or 6 weeks.
  • treatment of a subject with a therapeutically effective amount of a protein, polypeptide, or antibody can include a single treatment or, preferably, can include a series of treatments.
  • polypeptide formulations featured herein is a method for treating type II diabetes in a subject, which comprises contacting one or more cells in the subject with a first polypeptide, where the subject comprises a second polypeptide having one or more polymorphic variations associated with cancer, and where the first polypeptide comprises fewer polymorphic variations associated with cancer than the second polypeptide.
  • the first and second polypeptides are encoded by a nucleic acid which comprises a nucleotide sequence in SEQ ID NO: 1-20; a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence referenced in SEQ ID NO: 1-20; a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ED NO: 1-20 and a nucleotide sequence 90% or more identical to a nucleotide sequence in SEQ ID NO: 1-20.
  • the subject often is a human.
  • a dosage of 0.1 mg/kg of body weight (generally 10 mg/kg to 20 mg/kg) is often utilized. If the antibody is to act in the brain, a dosage of 50 mg/kg to 100 mg/kg is often appropriate. Generally, partially human antibodies and fully human antibodies have a longer half- life within the human body than other antibodies. Accordingly, lower dosages and less frequent administration is often possible. Modifications such as lipidation can be used to stabilize antibodies and to enhance uptake and tissue penetration (e.g., into the brain). A method for lipidation of antibodies is described by Cruikshank et al, J. Acquired Immune Deficiency Syndromes and Human Retrovirology 14:193 (1997).
  • Antibody conjugates can be used for modifying a given biological response, the drug moiety is not to be construed as limited to classical chemical therapeutic agents.
  • the drug moiety may be a protein or polypeptide possessing a desired biological activity.
  • proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, .alpha. -interferon, .beta.
  • an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Patent No. 4,676,980.
  • exemplary doses include milligram or microgram amounts of the compound per kilogram of subject or sample weight, for example, about 1 microgram per kilogram to about 500 milligrams per kilogram, about 100 micrograms per kilogram to about 5 milligrams per kilogram, or about 1 microgram per kilogram to about 50 micrograms per kilogram. It is understood that appropriate doses of a small molecule depend upon the potency of the small molecule with respect to the expression or activity to be modulated.
  • a physician, veterinarian, or researcher may, for example, prescribe a relatively low dose at first, subsequently increasing the dose until an appropriate response is obtained.
  • the specific dose level for any particular animal subject will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, gender, and diet of the subject, the time of administration, the route of administration, the rate of excretion, any drug combination, and the degree of expression or activity to be modulated.
  • gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration ⁇ see, e.g., U.S. Patent 5,328,470) or by stereotactic injection ⁇ see e.g., Chen et al, (1994) Proc. Natl Acad. ScL USA i?i:3054-3057).
  • Pharmaceutical preparations of gene therapy vectors can include a gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded.
  • the pharmaceutical preparation can include one or more cells which produce the gene delivery system. Examples of gene delivery vectors are described herein.
  • a therapeutic formulation described above can be administered to a subject in need of a therapeutic for inducing a desired biological response.
  • Therapeutic formulations can be administered by any of the paths described herein. With regard to both prophylactic and therapeutic methods of treatment, such treatments may be specifically tailored or modified, based on knowledge obtained from pharmacogenomic analyses described herein.
  • treatment is defined as the application or administration of a therapeutic formulation to a subject, or application or administration of a therapeutic agent to an isolated tissue or cell line from a subject with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improye or affect type II diabetes, symptoms of type II diabetes or a predisposition towards type II diabetes.
  • a therapeutic formulation includes, but is not limited to, small molecules, peptides, antibodies, ribozymes and antisense oligonucleotides.
  • Administration of a therapeutic formulation can occur prior to the manifestation of symptoms characteristic of type II diabetes, such that type II diabetes is prevented or delayed in its progression.
  • the appropriate therapeutic composition can be determined based on screening assays described herein.
  • embodiments include methods of causing or inducing a desired biological response in an individual comprising the steps of: providing or administering to an individual a composition comprising a polypeptide described herein, or a fragment thereof, or a therapeutic formulation described herein, wherein said biological response is selected from the group consisting of: (a) modulating circulating (either blood, serum or plasma) levels (concentration) of glucose, wherein said modulating is preferably lowering; (b) increasing cell or - tissue sensitivity to insulin, particularly muscle, adipose, liver or brain; (c) inhibiting the progression from impaired glucose tolerance to insulin resistance; (d) increasing glucose uptake in skeletal muscle cells; (e) increasing glucose uptake in adipose cells; (f) increasing glucose uptake in neuronal cells; (g) increasing glucose uptake in red blood cells; (h) increasing glucose uptake in the brain; and (i) significantly reducing the postprandial increase in plasma glucose following a meal, particularly a high carbohydrate meal
  • a pharmaceutical or physiologically acceptable composition can be utilized as an insulin sensitizer, or can be used in: a method to improve insulin sensitivity in some persons with type II diabetes in combination with insulin therapy; a method to improve insulin sensitivity in some persons with type II diabetes without insulin therapy; or a method of treating individuals with gestational diabetes.
  • Gestational diabetes refers to the development of diabetes in an individual during pregnancy, usually during the second or third trimester of pregnancy.
  • the pharmaceutical or physiologically acceptable composition can be used in a method of treating individuals with impaired fasting glucose (IFG).
  • Impaired fasting glucose (IFG) is a condition in which fasting plasma glucose levels in an individual are elevated but not diagnostic of overt diabetes (i.e.
  • the pharmaceutical or physiologically acceptable composition can be used in a method of treating and preventing impaired glucose tolerance (IGT) in an individual.
  • IGT impaired glucose tolerance
  • the pharmaceutical or physiologically acceptable composition can be used in a method of treating a subject having polycystic ovary syndrome (PCOS).
  • PCOS polycystic ovary syndrome
  • PCOS is among the most common disorders of premenopausal women, affecting 5-10% of this population.
  • Insulin-sensitizing agents e.g., troglitazone
  • troglitazone have been shown to be effective in PCOS and that, in particular, the defects in insulin action, insulin secretion, ovarian steroidogenesis and fibrinolysis are improved (Ehrman et al. (1997) J Clin Invest 100:1230), such as in insulin- resistant humans. Accordingly, provided are methods for reducing insulin resistance, normalizing blood glucose thus treating and/or preventing PCOS.
  • the pharmaceutical or physiologically acceptable composition can be used in a method of treating a subject having insulin resistance, where a subject having insulin resistance is treated to reduce or cure the insulin resistance.
  • a subject having insulin resistance is treated to reduce or cure the insulin resistance.
  • insulin resistance is also often associated with infections and cancer, preventing or reducing insulin resistance may prevent or reduce infections and cancer.
  • the pharmaceutical compositions and methods described herein are useful for: preventing the development of insulin resistance in a subject, e.g., those known to have an increased risk of developing insulin resistance; controlling blood glucose in some persons with type II diabetes in combination with insulin therapy; increasing cell or tissue sensitivity to insulin, particularly muscle, adipose, liver or brain; inhibiting or preventing the progression from impaired glucose tolerance to insulin resistance; improving glucose control of type II diabetes patients alone, without an insulin secretagogue or an insulin sensitizing agent; and administering a complementary therapy to type II diabetes patients to improve their glucose control in combination with an insulin secretagogue (preferably oral form) or an insulin sensitizing (preferably oral form) agent.
  • an insulin secretagogue preferably oral form
  • an insulin sensitizing preferably oral form
  • the oral insulin secretagogue sometimes is l,l-dimethyl-2-(2- morpholino phenyl)guanidine fumarate (BTS67582) or a sulphonylurea selected from tolbutamide, tolazamide, chlorpropamide, glibenclamide, glimepiride, glipizide and glidazide.
  • the insulin sensitizing agent sometimes is selected from metformin, ciglitazone, troglitazone and pioglitazone.
  • FIG. 1 For embodiments, further embodiments include methods of administering a pharmaceutical or physiologically acceptable composition concomitantly or concurrently, with an insulin secretagogue or insulin sensitizing agent, for example, in the form of separate dosage units to be used simultaneously, separately or sequentially (e.g., before or after the secretagogue or before or after the sensitizing agent).
  • a pharmaceutical or physiologically acceptable composition and an insulin secretagogue or insulin sensitizing agent as a combined preparation for simultaneous, separate or sequential use for the improvement of glucose control in type II diabetes patients.
  • any test known in the art or a method described herein can be used to determine that a subject is insulin resistant, and an insulin resistant patient can then be treated according to the methods described herein to reduce or cure the insulin resistance.
  • the methods described herein also can be used to prevent the development of insulin resistance in a subject, e.g., those known to have an increased risk of developing insulin-resistance.
  • modulators include, but are not limited to, small organic or inorganic molecules; antibodies (including, for example, polyclonal, monoclonal, humanized, anti-idiotypic, chimeric or single chain antibodies, and Fab, F(ab') 2 and Fab expression library fragments, scFV molecules, and epitope-binding fragments thereof); and peptides, phosphopeptides, or polypeptides.
  • antisense and ribozyme molecules that inhibit expression of the target gene can also be used to reduce the level of target gene expression, thus effectively reducing the level of target gene activity.
  • triple helix molecules can be utilized in reducing the level of target gene activity.
  • Antisense, ribozyme and triple helix molecules are discussed above. It is possible that the use of antisense, ribozyme, and/or triple helix molecules to reduce or inhibit mutant gene expression can also reduce or inhibit the transcription (triple helix) and/or translation (antisense, ribozyme) of mRNA produced by normal target gene alleles, such that the concentration of normal target gene product present can be lower than is necessary for a normal phenotype.
  • nucleic acid molecules that encode and express target gene polypeptides exhibiting normal target gene activity can be introduced into cells via gene therapy method.
  • the target gene encodes an extracellular polypeptide
  • nucleic acid molecules may be utilized in treating or preventing type II diabetes.
  • Aptamers are nucleic acid molecules having a tertiary structure which permits them to specifically bind to ligands (see, e.g., Osborne, et al, Curr. Opin. Chem. Biol.1(1): 5-9 (1997); and Patel, D. J., Curr. Opin. Chem. Biol. Jim; 1(1): 32-46 (1997)).
  • gene therapy which can also be referred to as allele therapy.
  • a gene therapy method for treating type II diabetes in a subject which comprises contacting one or more cells in the subject or from the subject with a nucleic acid having a first nucleotide sequence.
  • Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with type II diabetes (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO: 1-20).
  • the first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with type II diabetes than the second nucleotide sequence.
  • the first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof.
  • the subject is often a human. Allele therapy methods often are utilized in conjunction with a method of first determining whether a subject has genomic DNA that includes polymorphic variants associated with type II diabetes.
  • Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with type II diabetes (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO: 1-20).
  • the first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with type II diabetes than the second nucleotide sequence.
  • the first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof. The subject is often a human.
  • antibodies can be generated that are both specific for target molecules and that reduce target molecule activity. Such antibodies may be administered in instances where antagonizing a target molecule function is appropriate for the treatment of type II diabetes.
  • the target molecule is intracellular and whole antibodies are used, internalizing antibodies may be preferred.
  • Lipofectin or liposomes can be used to deliver the antibody or a fragment of the Fab region that binds to the target antigen into cells. Where fragments of the antibody are used, the smallest inhibitory fragment that binds to the target antigen is preferred. For example, peptides having an amino acid sequence corresponding to the Fv region of the antibody can be used. Alternatively, single chain neutralizing antibodies that bind to intracellular target antigens can also be administered.
  • Such single chain antibodies can be administered, for example, by expressing nucleotide sequences encoding single-chain antibodies within the target cell population (see, e.g., Marasco et ah, Proc. Natl. Acad. ScL USA 90: 7889- 7893 (1993)).
  • Modulators can be administered to a patient at therapeutically effective doses to treat type ⁇ diabetes.
  • a therapeutically effective dose refers to an amount of the modulator sufficient to result in amelioration of symptoms of type II diabetes.
  • Toxicity and therapeutic efficacy of modulators can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD 50 (the dose lethal to 50% of the population) and the ED 5O (the dose therapeutically effective in 50% of the population).
  • the dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD 50 ZED 50 .
  • Modulators that exhibit large therapeutic indices are preferred. While modulators that exhibit toxic side effects can be used, care should be taken to design a delivery system that targets such molecules to the site of affected tissue in order to minimize potential damage to uninfected cells, thereby reducing side effects.
  • Data obtained from cell culture assays and animal studies can be used in formulating a range of dosages for use in humans.
  • the dosage of such compounds lies preferably within a range of circulating concentrations that include the ED 50 with little or no toxicity.
  • the dosage can vary within this range depending upon the dosage form employed and the route of administration utilized.
  • the therapeutically effective dose can be estimated initially from cell culture assays.
  • a dose can be fo ⁇ nulated in animal models to achieve a circulating plasma concentration range that includes the IC 50 (i.e., the concentration of the test compound that achieves a half-maximal inhibition of symptoms) as determined in cell culture.
  • IC 50 i.e., the concentration of the test compound that achieves a half-maximal inhibition of symptoms
  • levels in plasma can be measured, for example, by high performance liquid chromatography.
  • Another example of effective dose determination for an individual is the ability to directly assay levels of "free" and "bound” compound in the serum of the test subject.
  • Such assays may utilize antibody mimics and/or "biosensors” that have been created through molecular imprinting techniques.
  • Molecules that modulate target molecule activity are used as a template, or "imprinting molecule”, to spatially organize polymerizable monomers prior to their polymerization with catalytic reagents. The subsequent removal of the imprinted molecule leaves a polymer matrix which contains a repeated "negative image" of the compound and is able to selectively rebind the molecule under biological assay conditions.
  • Such "imprinted" affinity matrixes can also be designed to include fluorescent groups whose photon-emitting properties measurably change upon local and selective binding of target compound. These changes readily can be assayed in real time using appropriate fiberoptic devices, in turn allowing the dose in a test subject to be quickly optimized based on its individual IC 50 .
  • An example of such a "biosensor” is discussed in Kriz et at, Analytical Chemistry 67: 2142-2144 (1995).
  • SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLlS polypeptides and polypeptide variants in vitro or in vivo.
  • SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAMS and GLlS nucleic acids or polypeptides and variants thereof are utilized for screening test molecules for those that interact with SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLIS molecules.
  • Test molecules identified as interactors with SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLIS molecules and variants are further screened in vivo to determine whether they treat breast cancer.
  • Example 1 Test molecules identified as interactors with SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLIS molecules and variants are further screened in vivo to determine whether they treat breast cancer.
  • Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to as case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age-matched controls. A database was created that listed all phenotypic trait information gathered from individuals for each case and control sample. Genomic DNA was extracted from each of the blood samples for genetic analyses.
  • the solution was incubated at 37°C or room temperature if cell clumps were visible after mixing until the solution was homogeneous.
  • 2 ml of protein precipitation was added to the cell lysate.
  • the mixtures were vortexed vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate, and then centrifuged for 10 minutes at 3000 x g.
  • the supernatant containing the DNA was then poured into a clean 15 ml tube, which contained 7 ml of 100% isopropanol.
  • the samples were mixed by inverting the tubes gently until white threads of DNA were visible.
  • DNA was quantified by placing samples on a hematology mixer for at least 1 hour. DNA was serially diluted (typically 1:80, 1:160, 1:320, and 1:640 dilutions) so that it would be within the measurable range of standards. 125 ⁇ l of diluted DNA was transferred to a clear U- bottom microtitre plate, and 125 ⁇ l of IX TE buffer was transferred into each well using a multichannel pipette. The DNA and IX TE were mixed by repeated pipetting at least 15 times, and then the plates were sealed. 50 ⁇ l of diluted DNA was added to wells A5-H12 of a black flat bottom microtitre plate.
  • DNA was serially diluted (typically 1:80, 1:160, 1:320, and 1:640 dilutions) so that it would be within the measurable range of standards.
  • 125 ⁇ l of diluted DNA was transferred to a clear U- bottom microtitre plate, and 125 ⁇ l of IX TE buffer was
  • the plate was placed into a Fluoroskan Ascent Machine (microplate fluorometer produced by Labsystems) and the samples were allowed to incubate for 3 minutes before the machine was run using filter pairs 485 nm excitation and 538 nm emission wavelengths. Samples having measured DNA concentrations of greater than 450 ng/ ⁇ l were re-measured for conformation. Samples having measured DNA concentrations of 20 ng/ ⁇ l or less were re-measured for confirmation.
  • a Fluoroskan Ascent Machine microplate fluorometer produced by Labsystems
  • Samples were placed into one of four groups based on disease status.
  • the four groups were female case samples, female control samples, male case samples and male control samples.
  • a select set of samples from each group were utilized to generate pools, and one pool was created for each group.
  • Each individual sample in a pool was represented by an equal amount of genomic DNA. For example, where 25 ng of genomic DNA was utilized in each PCR reaction and there were 200 individuals in each pool, each individual would provide 125 pg of genomic DNA.
  • Inclusion or exclusion of samples for a pool was based upon the following criteria and detailed in the tables below: patient ethnicity, diagnosis with type II diabetes, GAD antibody concentration, HbAIc concentration, body mass (BMI), patient age, date of primary diagnosis, and age of individual as of primary diagnosis.
  • a whole-genome screen was performed to identify particular SNPs associated with occurrence of type II diabetes. As described in Example 1, two sets of samples were utilized: female individuals having type II diabetes (female cases) and samples from female individuals not having type II diabetes or any history of type II diabetes (female controls), and male individuals having type II diabetes (male cases) and samples from male individuals not having type II diabetes or any history of type II diabetes (male controls).
  • the initial screen of each pool was performed in an allelotyping study, in which certain samples in each group were pooled. By pooling DNA from each group, an allele frequency for each SNP in each group was calculated. These allele frequencies were then compared to one another.
  • SNP disease association results obtained from the allelotyping study were then validated by genotyping each associated SNP across all samples from each pool. The results of the genotyping were then analyzed, allele frequencies for each group were calculated from the individual genotyping results, and a p-value was calculated to determine whether the case and control groups had statistically significantly differences in allele frequencies for a particular SNP. When the genotyping results agreed with the original allelotyping results, the SNP disease association was considered validated at the genetic level.
  • a whole-genome SNP screen began with an initial screen of approximately 25,000 SNPs over each set of disease and control samples using a pooling approach. The pools studied in the screen are described in Example 1.
  • the SNPs analyzed in this study were part of a set of 25,488 SNPs confirmed as being statistically polymorphic as each is characterized as having a minor allele frequency of greater than 10%.
  • the SNPs in the set reside in genes or in close proximity to genes, and many reside in gene exons. Specifically, SNPs in the set are located in exons, introns, and within 5,000 base-pairs upstream of a transcription start site of a gene.
  • SNPs were selected according to the following criteria: they are located in ESTs; they are located in Locuslink or Ensembl genes; and they are located in Genomatix promoter predictions. SNPs in the set were also selected on the basis of even spacing across the genome, as depicted in Table 3. An additional 3088 SNPs were included with these 25,488 SNPs and these additional SNPs had been chosen on the basis of gene location, with preference to non-synonymous coding SNPs located in disease candidate genes.
  • Table 4 includes information pertaining to the incident polymorphic variant associated with type II diabetes identified herein. Public information pertaining to the polymorphism and the genomic sequence that includes the polymorphism are indicated. The genomic sequences identified in Table 4 may be accessed at the http address www.ncbi.nih.gov/entrez/query.fcgi, for example, by using the publicly available SNP reference number (e.g., rs662138).
  • the "Contig Position” provided in Table 4 corresponds to a nucleotide position set forth in the contig sequence, and designates the polymorphic site corresponding to the SNP reference number.
  • the sequence containing the polymorphisms also may be referenced by the "Sequence Identification" set forth in Table 4.
  • the "Sequence Identification” corresponds to cDNA sequence that encodes associated target polypeptides (e.g., SLC22A1) of the invention.
  • the position of the SNP within the cDNA sequence is provided in the "Sequence Position" column of Table 4. Also, the allelic variation at the polymorphic site and the allelic variant identified as associated with type II diabetes is specified in Table 4. All nucleotide sequences referenced and accessed by the parameters set forth in Table 4 are incorporated herein by reference.
  • a MassARRAY® system (Sequenom, Inc.) was utilized to perform SNP genotyping in a high-throughput fashion. This genotyping platform was complemented by a homogeneous, single-tube assay method (hMETM or homogeneous MassEXTENDTM (Sequenom, Inc.)) in which two genotyping primers anneal to and amplify a genomic target surrounding a polymorphic site of interest. A third primer (the MassEXTENDTM primer), which is complementary to the amplified target up to but not including the polymorphism, was then enzymatically extended one or a few bases through the polymorphic site and then terminated.
  • hMETM homogeneous, single-tube assay method
  • MassEXTENDTM primer which is complementary to the amplified target up to but not including the polymorphism
  • SpectroDESIGNERTM software (Sequenom, Inc.) was used to generate a set of PCR primers and a MassEXTENDTM primer was used to genotype the polymorphism.
  • Table 5 shows PCR primers and Table 6 shows extension primers used for analyzing polymorphisms.
  • the initial PCR amplification reaction was performed in a 5 ⁇ l total volume containing IX PCR buffer with 1.5 mM MgCl 2 (Qiagen), 200 ⁇ M each of dATP, dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units of HotStar DNA polymerase (Qiagen), and 200 nM each of forward and reverse PCR primers specific for the polymorphic region of interest.
  • Qiagen PCR Primers
  • a primer extension reaction was initiated by adding a polymorphism-specific MassEXTENDTM primer cocktail to each sample.
  • Each MassEXTENDTM cocktail included a specific combination of dideoxynucleotides (ddNTPs ⁇ and deoxynucleotides (dNTPs) used to distinguish polymorphic alleles from one another.
  • ddNTPs dideoxynucleotides
  • dNTPs deoxynucleotides
  • the MassEXTENDTM reaction was performed in a total volume of 9 ⁇ l, with the addition of IX ThermoSequenase buffer, 0.576 units of ThermoSequenase (Amersham Pharmacia), 600 nM MassEXTENDTM primer, 2 mM of ddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTP or dGTP or dTTP.
  • the deoxy nucleotide (dNTP) used in the assay normally was complementary to the nucleotide at the polymorphic site in the amplicon. Samples were incubated at 94°C for 2 minutes, followed by 55 cycles of 5 seconds at 94°C, 5 seconds at 52°C, and 5 seconds at 72°C.
  • samples were desalted by adding 16 ⁇ l of water (total reaction volume was 25 ⁇ l), 3 mg of SpectroCLEANTM sample cleaning beads (Sequenom, Inc.) and allowed to incubate for 3 minutes with rotation. Samples were then robotically dispensed using a piezoelectric dispensing device (SpectroJETTM (Sequenom, Inc.)) onto either 96-spot or 384-spot silicon chips containing a matrix that crystallized each sample (SpectroCFflP ® (Sequenom, Inc.)).
  • MALDI-TOF mass spectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (Bruker Daltonics) can be used) and SpectroTYPER RTTM software (Sequenom, Inc.) were used to analyze and interpret the SNP genotype for each sample.
  • Genotyping results for the allelic variant set forth in Table 4 are shown for female pools in Table 7, for male pools in Table 8, and the combined results in Table 9.
  • F case and F control refer to female case and female control groups
  • M case and M control refer to male case and male control groups
  • AF refers to allele frequency.
  • SNP rs366111 is a coding non-synonymous SNP (G/A), which results in an amino acid change from arginine to glycine.
  • G/A coding non-synonymous SNP
  • the G allele is. associated with an increased risk of type II diabetes and codes for glycine; therefore, individuals with the glycine residue also have an increased risk of type II diabetes.
  • Odds ratio results are shown in Tables 7, 8 and 9. An odds ratio is an unbiased estimate of relative risk which can be obtained from most case-control studies.
  • Relative risk (RR) is an estimate of the likelihood of disease in the exposed group (susceptibility allele or genotype carriers) compared to the unexposed group (not carriers). It can be calculated by the following equation:
  • /A is the incidence of disease in the A carriers and /a is the incidence of disease in the non- carriers.
  • RR > 1 indicates the A allele increases disease susceptibility.
  • RR ⁇ 1 indicates the a allele increases disease susceptibility.
  • An odds ratio can be interpreted in the same way a relative risk is interpreted and can be directly estimated using the data from case-control studies, i.e., case and control allele frequencies.
  • the higher the odds ratio value the larger the effect that particular allele has on the development of type II diabetes. Possessing an allele associated with a relatively high odds ratio translates to having a higher risk of developing or having type II diabetes.
  • the single marker polymorphisms set forth in Table 4 were genotyped again in two replication cohorts to further validate their association with type II diabetes. Like the original study population described in Examples 1 and 2, the replication cohorts consisted of type II diabetics (cases.) and non-diabetics (controls). The case and control samples were selected and genotyped as described below.
  • Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to as case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age-matched controls. All of the samples were collected from individuals residing in Newfoundland, Canada. residents of Newfoundland represent a preferred population for genetic studies because of their relatively small founder population and resulting homogeneity.
  • Phenotypic trait information was gathered from individuals for each case and control sample, and genomic DNA was extracted from each of the blood samples for genetic analyses.
  • Samples were placed into one of four groups based on disease status. The four groups were female case samples, female control samples, male case samples, and male control samples. A select set of samples from each group were utilized to generate pools, and one pool was created for each group.
  • Patients were included in the case pools if a) they were diagnosed with type II diabetes as documented in their medical record, b) they were treated with either insulin or oral hypoglycaemic agents, and c) they were of Caucasian ethnicity. Patients were excluded in the case pools if a) they were diabetic or had a history of diabetes, b) they suffered from diet controlled glucose intolerance, or c) they (or any their relatives) were diagnosed with MODY or gestational diabetes.
  • Phenotype information included, among others, patient ethnicity, country or origin of mother and father, diagnosis with type II diabetes (date of primary diagnosis, age of individual as of primary diagnosis), body weight, onset of obesity, retinopathy, glaucoma, cataracts, nephropathy, heart disease, hypertension, myocardial infarction, ulcers, required treatment (onset of insulin treatment, oral hypoglycemic agent), blood glucose levels, and MODY.
  • the polymorphisms described in Table 4 were genotyped again in a second replication cohort, consisting of individuals of Danish ancestry, to further validate their association with type II diabetes. Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age- matched controls.
  • Phenotypic trait information was gathered from individuals for each case and control sample, and genomic DNA was extracted from each of the blood samples for genetic analyses.
  • Samples were placed into one of four groups based on disease status. The four groups were female case samples, female control samples, male case samples, and male control samples. A select set of samples from each group were utilized to generate pools, and one pool was created for each group.
  • Phenotype information included, among others, e.g. body mass index , waist/hip ratio, blood pressure, serum insulin, glucose, C-peptide, cholesterol, hdl, triglyceride, Hb A ic, urine, creatinine, free fatty acids (mmol/1), GAD antibodies.
  • the associated SNP from the initial scan was re-validated by genotyping the associated SNP across the replication cohorts described in Example 3. The results of the genotyping were then analyzed, allele frequencies for each group were calculated from the individual genotyping results, and p-values were calculated to determine whether the case and control groups had statistically significant differences in allele frequencies for a particular SNP.
  • the replication genotyping results with a calculated p-value of less than 0.05 were considered particularly significant, which are set forth in bold text. See Tables 12-14 herein.
  • Genotyping of the replication cohort was performed using the same methods used for the original genotyping, as described herein.
  • a MassARRAY® system (Sequenom, Inc.) was utilized to perform SNP genotyping in a high-throughput fashion.
  • This genotyping platform was complemented by a homogeneous, single-tube assay method (hMETM or homogeneous MassEXTENDTM (Sequenom, Inc.)) in which two genotyping primers anneal to and amplify a genomic target surrounding a polymorphic site of interest.
  • a third primer (the MassEXTENDTM primer), which is complementary to the amplified target up to but not including the polymorphism, was then enzymatically extended one or a few bases through the polymorphic site and then terminated.
  • SpectroDESIGNERTM software (Sequenom, Inc.) was used to generate a set of PCR primers and a MassEXTENDTM primer which where used to genotype the polymorphism.
  • Other primer design software could be used or one of ordinary skill in the art could manually design primers based on his or her knowledge of the relevant factors and considerations in designing such primers.
  • Table 6 shows PCR primers and Table 7 shows extension probes used for analyzing (e.g., genotyping) polymorphisms in the replication cohorts.
  • the initial PCR amplification reaction was performed in a 5 ⁇ l total volume containing IX PCR buffer with 1.5 mM MgCl 2 (Qiagen), 200 ⁇ M each of dATP, dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units of HotStar DNA polymerase (Qiagen), and 200 nM each of forward and reverse PCR primers specific for the polymorphic region of interest. [0255] Samples were incubated at 95°C for 15 minutes, followed by 45 cycles of 95 0 C for 20 seconds, 56 0 C for 30 seconds, and 72°C for 1 minute, finishing with a 3 minute final extension at 72°C.
  • shrimp alkaline phosphatase (0.3 units in a 2 ⁇ l volume) (Amersham Pharmacia) was added to each reaction (total reaction volume was 7 ⁇ l) to remove any residual dNTPs that were not consumed in the PCR step. Samples were incubated for 20 minutes at 37°C, followed by 5 minutes at 85°C to denature the SAP.
  • SAP shrimp alkaline phosphatase
  • a primer extension reaction was initiated by adding a polymorphism-specific MassEXTENDTM primer cocktail to. each sample.
  • Each MassEXTENDTM cocktail included a specific combination of dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs) used to distinguish polymorphic alleles from one another.
  • ddNTPs dideoxynucleotides
  • dNTPs deoxynucleotides
  • the MassEXTENDTM reaction was performed in a total volume of 9 ⁇ l, with the addition of IX ThermoSequenase buffer, ⁇ .576 units of ThermoSequenase (Amersham Pharmacia), 600 nM MassEXTENDTM primer, 2 mM of ddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTP or dGTP or dTTP.
  • the deoxy nucleotide (dNTP) used in the assay normally was complementary to the nucleotide at the polymorphic site in the amplicon. Samples were incubated at 94°C for 2 minutes, followed by 55 cycles of 5 seconds at 94°C, 5 seconds at 52°C, and 5 seconds at 72°C.
  • samples were desalted by adding 16 ⁇ l of water (total reaction volume was 25 ⁇ l), 3 mg of SpectroCLEANTM sample cleaning beads (Sequenom, Inc.) and allowed to incubate for 3 minutes with rotation. Samples were then robotically dispensed using a piezoelectric dispensing device (SpectroJETTM (Sequenom, Inc.)) onto either 96-spot or 384-s.pot silicon chips containing a matrix that crystallized each sample (SpectroCHIP® (Sequenom, Inc.)).
  • MALDI-TOF mass spectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (Bruker Daltonics) can be used) and SpectroTYPER RTTM software (Sequenom, Inc.) were used to analyze and interpret the SNP genotype for each sample.
  • TBN refers to the discovery cohort
  • NNL refers to the Newfoundland replication cohort
  • Steno refers to the Denmark replication cohort.
  • the boxes are centered over the odds ratio for each sample, with the size of the box correlated to the contribution of each sample to the combined meta analysis odds ratio.
  • the lines extending from each box are the 95% confidence interval values.
  • the diamond is centered over the combined meta analysis odds ratio with the ends of the diamond depicting the 95% confidence interval values.
  • the meta-analysis further illustrates the strong association each of the incident SNPs has with type II diabetes across multiple case and control samples.
  • the SNP rs662138 associated with type II diabetes lies within intron 7 of SLC22A1.
  • Ninety additional allelic variants proximal to rs662138 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 14.
  • the chromosome position provided in column four of Table 14 is based on Genome "Build 34" of NCBFs GenBank.
  • the "genome letter” corresponds to the particular allele that appears in NCBFs build 34 genomic sequence of the region (chromosome 6: positions 160423601-160523200), and the “deduced iupac” corresponds to the single letter IUPAC code for the SLC22A1 polymorphic variants as they appear in SEQ ID NO: 1. Also, the “genome letter” may differ from the alleles (A1/A2) provided in Table 14 (and in subsequent Tables that provide the same information) if the genome letter is on one strand and the alleles are on the complementary strand, thus having different strand orientations ⁇ i.e., reverse vs forward). TABLE 14
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures IA- 1C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 1A-1C can be determined by consulting Tables 17-19.
  • the left-most X on the left graph is at position 160423829.
  • the allele frequency associated with each symbol shown can be determined.
  • multiple lines have been added to the graph.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 20, 21 and 22 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • Metformin seems to ameliorate hyperglycemia by improving peripheral sensitivity to insulin, reducing gastrointestinal glucose absorption and hepatic glucose production (Caspary and Creutzfeldt, Diabetologia 7: 379-385, 1971; ⁇ un ⁇ al et aL, 2000 Diabetes 49: 2063-2069; Borst and Snellen, 2001 Life Sd 69: 1497- 1507), although the exact mechanism for its pharmacological action is not yet fully understood.
  • Table 23 shows that the major allele C has been selected for through evolution, maybe in times of lack of substantial food, as happened with the Pima Indians. The results show that having the major allele C individuals have a greater risk of developing diabetes. In addition, they are less likely to be treated with metformin. Accordingly, it is believed that patients were treated and did not respond. Also, it is believed that the polymorphism in the SLC22A1 gene results, in an alteration in the metformin uptake and or signaling cascade thereby preventing the substrate of SLC22A1 binding. Usually when metformin is administered to diabetics it results in a decrease in hepatic glucose production (HGP). This decrease in HGP may not be seen in the individual with the polymorphism in SLC22A1. Thus administering the drug to these individuals would result in no overall improvement in glucose homeostasis.
  • HGP hepatic glucose production
  • the SNP rs 1001238 is associated with type II diabetes in the examples above.
  • One hundred thirty-four additional allelic variants proximal to rs 1001238 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 24.
  • the chromosome position provided in column four of Table 24 is based on Genome "Build 34" of NCBPs GenBank.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 2A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 2A-C can be determined by consulting Tables 27-29. For example, the left-most X on the left graph is at position 179567944. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 "8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples.3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 30, 31 and 32 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • the SNP rs3219 associated with type II diabetes in the examples above falls in the untranslated region of the LOCI 12609 gene. Fifty-one additional allelic variants proximal to rs3219 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 33. The chromosome position provided in column four of Table 33 is based on Genome "Build 34" of NCBPs GenBank.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 3A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 3A-C can be determined by consulting Tables 36-38. For example, the left-most X on the left graph is at position 84746396. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances, between SNPs.
  • Two other lines are drawn to expose linear trends, in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 "8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping, results are shown for female (F), male (M), and combined cases and controls in Table 39, 40 and 41 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • Some SNPs may be labeled "untyped" because of failed assays. TABLE 39: Female Replication Allelotyping Results
  • the SNP rsl 160333 associated with type II diabetes in the examples above falls within an intron of the trichorhinophalangeal syndrome I (TRPSl) gene.
  • TRPSl trichorhinophalangeal syndrome I
  • Sixty additional allelic variants proximal to rsl 160333 were identified and subsequently allelotyped in diabetes, case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 42.
  • the chromosome position provided in column four of Table 42 is based on Genome "Build 34" of NCBFs GenBank.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 4A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives, the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 4A-C can be determined by consulting Tables 45-47. For example, the left-most X on the left graph is at position 116488511. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 "8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 48, 49 and 50 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • the SNP rs 1024373 associated with type II diabetes in the examples above falls within an intron of the PRAME gene.
  • One hundred thirty-two additional allelic variants proximal to rslO24373 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 51.
  • the chromosome position provided in column four of Table 51 is based on Genome "Build 34" of NCBFs GenBank.
  • the amino acid changes provided in column 8 of Table 51 correspond to SNPs that code for non-synonymous amino acid changes, where changes with two * occur in the SUHW2 polypeptide (locus ID no. 140883), changes with one * occur in the SUHWl polypeptide (locus ID no. 129025), and the W7R change occurs in the PRAME polypeptide.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 5A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures.5 A-C can be determined by consulting Tables 54-56. For example, the left-most X on the left graph is at position 21166760. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 "8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 57, 58 and 59 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • SNP rs366111 associated with type II diabetes in the examples above falls within an exon of the ZNF221 gene.
  • SNP r&366111 is a coding non-synonymous SNP (G/A), which results in an amino acid change from arginine to glycine.
  • G/A coding non-synonymous SNP
  • the G allele is associated with an increased risk of type II diabetes and codes for glycine; therefore, individuals with the glycine residue also have an increased risk of type II diabetes.
  • One hundred forty-six additional allelic variants proximal to rs366111 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 60.
  • the chromosome position provided in column four of Table 60 is based on Genome "Build 34" of NCBI's GenBank.
  • the amino acid changes provided in column 8 of Table 60 correspond to SNPs that code for non-synonymous amino acid changes, where changes with one * occur in the ZNF221 polypeptide, changes with two * occur in the ZNFl 55 polypeptide (locus ID no. 7711), and changes with three * occur in the ZNF230 polypeptide (locus ID no. 7773).
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 6A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 6A-C can be determined by consulting Tables 63-65. For example, the left-most X on the left graph is at position 49113509. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 "8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 66, 61 and 68 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • the SNP rsl203877 associated with type II diabetes in the examples above falls near a member of the forkhead class of DNA-binding proteins called forkhead box A2 (FOXA2).
  • FOXA2 forkhead box A2
  • One hundred additional allelic variants proximal to rs 1203877 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 69.
  • the chromosome position provided in column four of Table 69 is based on Genome "Build 34" of NCBI's GenBank.
  • allelotyping results were considered particularly significant with a calculated p- value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 7A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 7A-C can be determined by consulting Tables 12-1 A. For example, the left-most X on the left graph is at position 22487606. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10 '8 were truncated at that value.
  • the proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 75, 76 and 77 respectively.
  • the allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency.
  • Tables 81-83 shows the case and control allele frequencies along with the p- values for all of the SNPs genotyped in female pools, male pools, and combined, respectively. P- values less than 0.05 are in bold.
  • the SNP rs470936 associated with type II diabetes in the examples above falls within an intron of the JAMS gene.
  • One hundred ten additional allelic variants proximal to rs470936 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2.
  • the polymorphic variants are set forth in Table 84.
  • the chromosome position provided in column four of Table 84 is based on Genome "Build 34" of NCBI's GenBank.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 8A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 8A-C can be determined by consulting Tables 87-89. For example, the left-most X on the left graph is at position 133498018. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two common significance levels, and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs. to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and T.J. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than ICF 8 were truncated at that value.
  • SNP rs846312 associated with type II diabetes in the examples above falls within • an intron of the GLI3 gene. Two hundred fifteen additional allelic variants proximal to rs846312 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 90. SNP rs846266 codes for a non-synonymous amino acid change (Tl 83A). The chromosome position provided in column four of Table 90 is based on Genome "Build 34" of NCBFs GenBank.
  • allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold.
  • the allelotyping p-values were plotted in Figures 9A-C for females, males and combined, respectively.
  • the position of each SNP on the chromosome is presented on the x-axis.
  • the y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group.
  • the minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 9A-C can be determined by consulting Tables 93-95. For example, the left-most X on the left graph is at position 41819727. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
  • the broken horizontal lines are drawn at two. common significance levels, 0.05 and 0.01.
  • the vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs.
  • Two other lines are drawn to expose linear trends in the association of SNPs to the disease.
  • the light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.).
  • the black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p- values that were less than 10 "8 were truncated at that value.
  • cDNA is. cloned into a pIVEX 2.3-MCS vector (Roche Biochem ⁇ using a directional cloning method.
  • a cDNA insert is prepared using PCR with forward and reverse primers having 5' restriction site tags, (in frame) and 5-6 additional nucleotides in addition to 3' gene-specific portions, the latter of which is typically about twenty to about twenty-five base pairs in length.
  • a Sal I restriction site is introduced by the forward primer and a Sma I restriction site is introduced by the reverse primer.
  • the ends of PCR products are cut with the corresponding restriction enzymes (i.e., Sal I and Sma I) and the products are gel-purified.
  • the pIVEX 2.3-MCS vector is linearized using the same restriction enzymes, and the fragment with the correct sized fragment is isolated by gel-purification. Purified PCR product is ligated into the linearized pIVEX 2.3-MCS vector and E. coli cells transformed for plasmid amplification. The newly constructed expression vector is verified by restriction mapping and used for protein production.
  • E. coti lysate is reconstituted with 0.25 ml of Reconstitution Buffer, the Reaction Mix is reconstituted with 0.8 ml of Reconstitution Buffer; the Feeding Mix is reconstituted with 10.5 ml of Reconstitution Buffer; and the Energy Mix is reconstituted with 0.6 ml of Reconstitution Buffer.
  • 0.5 ml of the Energy Mix was added to the Feeding Mix to obtain the Feeding Solution.
  • 0.75 ml of Reaction Mix, 50 ⁇ l of Energy Mix, and 10 ⁇ g of the template DNA is added to the E. coli lysate.
  • reaction device (Roche Biochem) 1 ml of the Reaction Solution is loaded into the reaction compartment.
  • the reaction device is turned upside-down and 10 ml of the Feeding Solution is loaded into the feeding compartment. All lids are closed and the reaction device is loaded into the RTS500 instrument. The instrument is run at 30 0 C for 24 hours with a stir bar speed of 150 rpm.
  • the pIVEX 2.3 MCS vector includes a nucleotide sequence that encodes six consecutive histidine amino acids on the C-terminal end of the target polypeptide for the purpose of protein purification.
  • Target polypeptide is purified by contacting the contents of reaction device with resin modified with Ni 2+ ions.
  • Target polypeptide is eluted from the resin with a solution containing free Ni 2+ ions.
  • Nucleic acids are cloned into DNA plasmids having phage recombination cites and target polypeptides are expressed therefrom in a variety of host cells.
  • Alpha phage genomic DNA contains short sequences known as attP sites
  • E. coli genomic DNA contains unique, short sequences known as attB sites. These regions share homology, allowing for integration of phage DNA into E. coli via directional, site-specific recombination using the phage protein Int and the E. coli protein IHF. Integration produces two new art sites, L and R, which flank the inserted prophage DNA. Phage excision from E.
  • coli genomic DNA can also be accomplished using these two proteins with the addition of a second phage protein, Xis.
  • DNA vectors have been produced where the integration/excision process is modified to allow for the directional integration or excision of a target DNA fragment into a backbone vector in a rapid in vitro reaction (GatewayTM Technology (Invitrogen, Inc.)).
  • a first step is to transfer the nucleic acid insert into a shuttle vector that contains attL sites surrounding the negative selection gene, ccdB (e.g. pENTER vector, Invitrogen, Inc.). This transfer process is accomplished by digesting the nucleic acid from a DNA vector used for sequencing, and to ligate it into the multicloning site of the shuttle vector, which will place it between the two attL sites while removing the negative selection gene ccdB.
  • a second method is to amplify the nucleic acid by the polymerase chain reaction (PCR) with primers containing attB sites. The amplified fragment then is integrated into the shuttle vector using Int and IHF.
  • PCR polymerase chain reaction
  • a third method is to utilize a topoisomerase-mediated process, in which the nucleic acid is amplified via PCR using gene-specific primers with the 5' upstream primer containing an additional CACC sequence ⁇ e.g.,
  • the PCR amplified fragment can be cloned into the shuttle vector via the attL sites in the correct orientation.
  • the nucleic acid can be cloned into an expression vector having attR sites.
  • vectors containing attR sites, for expression of target polypeptide as a native polypeptide, N-fusion polypeptide, and C-fusion polypeptides are commercially available (e.g. , pDEST (Invitrogen, Inc.)), and any vector can be converted into an expression vector for receiving a nucleic acid from the shuttle vector by introducing an insert having an attR site flanked by an antibiotic resistant gene for selection using the standard methods described above. Transfer of the nucleic acid from the shuttle vector is accomplished by directional recombination using Int, IHF, and Xis (LR clonase).
  • the desired sequence can be transferred to an expression vector by carrying out a one hour incubation at room temperature with Int, IHF, and Xis, a ten minute incubation at 37°C with proteinase K, transforming bacteria and allowing expression for one hour, and then plating on selective media. Generally, 90% cloning efficiency is achieved by this method.
  • expression vectors are pDEST 14 bacterial expression vector with att7 promoter, pDEST 15 bacterial expression vector with a T7 promoter and a N-terminal GST tag, pDEST 17 bacterial vector with a T7 promoter and a N-terminal polyhistidine affinity tag, and pDEST 12.2 mammalian expression vector with a CMV promoter and neo resistance gene. These expression vectors or others like them are transformed or transfected into cells for expression of the target polypeptide or polypeptide variants. These expression vectors are often transfected, for example, into murine-transformed a adipocyte cell line 3T3-L1, (ATCC), human embryonic kidney cell line 293, and rat cardiomyocyte cell line H9C2.
  • test molecule refers to a molecule that is added to a system, where an agonist effect, antagonist effect, or lack of an effect of the molecule on SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 function or a related physiological function in the system is assessed.
  • An example of a test molecule is a test compound, such as a test compound described in the section "Compositions Comprising Diabetes-Directed Molecules" above.
  • test molecule is a test peptide, which includes, for example, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3- related test peptide such as a soluble, extracellular form of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLB, a biologically active fragment of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 binding partner or ligand, or a functional fragment of the foregoing.
  • a concentration range or amount of test molecule utilized in the assays and models is selected from a variety of available ranges and amounts.
  • a test molecule sometimes is introduced to an assay system in a concentration range between 1 nanomolar and 100 micromolar or a concentration range between 1 nanograms/mL and 100 micrograms/mL.
  • An effect of atest molecule on SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 function or a related physiological function often is determined by comparing an effect in a system administered the test molecule against an effect in system not admininstered the test molecule. Described directly hereafter are examples of in vitro assays. Glucose Uptake Assay
  • GLUT4 an insulin- regulatable glucose transporter.
  • Insulin binding to insulin receptors on the cell surface results in autophosphorylation and activation of the intrinsic tyrosine kinase activity of the insulin receptor.
  • Phosphorylated tyrosine residues on the insulin receptor and its endogenous targets activate several intracellular signaling pathways that eventually lead to the translocation of GLUT4 from intracellular stores to the extracellular membrane.
  • Cells are plated in 6-welI dishes, and grown to confluency. Cells are then differentiated with DMEM plus 10% fetal calf serum (FCS) 5 10 ug/mL insulin, 390 ng/mL dexamethasone and 112 ug/mL isobutylmethylxanthine for 2 days. After 2 days of differentiation * media is changed to maintenance media DMEM plus. 10% FCS and 5 ug/mL insulin. Media is changed every 2 days thereafter. Cells are assayed for insulin-mediated glucose uptake 10 days after differentiation.
  • FCS fetal calf serum
  • recombinant rat SLC22A1, TTN 3 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeria is added to a concentration of 1.75 ug/mL, and anti-human IgG, Fc ⁇ fragment specific antibody to a final concentration of 17.5 ug/mL.
  • media is. replaced with 2 mL of preclustered SLC22A1, TTN 5 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3, and incubated for 10, 40 and 90 min at 37 deg.
  • porcine insulin is added to a final concentration of 100 nM for 10 min at 37 deg.
  • 100 uL of PBS-2-DOG label is added to give a final concentration of 2 uCi.
  • Cells are immediately placed on ice, washed three times with ice cold PBS, and lysed with 0.7 mL of 0.2 N NaOH. Lysates are read in a Wallac 1450 Microbeta Liquid Scintillation and Luminescence Counter.
  • TGs triacylglycerol
  • a direct metabolic consequence of glucose transport intracellularly is its incorporation into the fatty acid and glycerol moieties of triacylglycerol (TG).
  • TGs are highly concentrated stores of metabolic energy, and are the major energy reservoir of cells.
  • the major site of accumulation of triacylglycerols is the cytoplasm of adipose cells.
  • Adipocytes are specialized for the synthesis, and storage of TG, and for their mobilization into fuel molecules that are transported to other tissues through the bloodstream. It is likely that changes in the transport of glucose intracellularly can affect cytoplasmic stores of triacylglycerols.
  • Cells are plated in 6-well dishes, and grown to confluency. When cells reached confluency, cells are differentiated with DMEM plus 10% fetal calf serum (FCS), 10 ug/mL insulin, 390 ng/mL dexamethasone and 112 ug/mL isobutylmethylxanthine for 2 days. After 2 days of differentiation, media is changed to maintenance media DMEM plus 10% FCS and 5 ug/mL insulin. On the day of the assay (day 9 post-differentiation), cells are washed once with PBS, and serum starved by adding 2 mL of DMEM plus 2 mg/niL BSA for 3 hours.
  • FCS fetal calf serum
  • recombinant rat SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeric ligand is preclustered.
  • a solution of PBS plus 2 mg/mL BSA recombinant rat SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeria is added to a concentration of 1.75 ug/mL, and anti-human IgG, Fc ⁇ fragment specific antibody to a final concentration of 17.5 ug/mL.
  • sample In a 96-well, flat bottom, transparent microtiter plate, 3 uL of sample are added to 300 uL of INFINITY Triglyceride Reagent. Samples are incubated at room temperature for 10 minutes. The assay is read at 500-550 nm.
  • Resistin is a secreted factor specifically expressed in white adipocyte. It was initially discovered in a screen for genes downregulated in adipocytes by PPAR gamma, and expression was found to be attenuated by insulin. Elevated levels of resistin have been measured in genetically obese, and high fat fed obese mice. It is therefore thought that resistin contributes to peripheral tissue insulin unresponsiveness, one of the pathological hallmarks of diabetes.
  • 3T3-L1 cells are differentiated for 3 days as previously described and maintained for three days prior to splitting. At day 5 post-differentiation, differentiated cells are plated in 10 cm dish at a cell density of 3X10 6 cells. Cells are then serum starved on day 7 after initiation of differentiation. On day 8, cells are treated with pre-clustered recombinant rat SLC22A1, TTN 5 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLBfFc chimera as described above for 10 min and treated with 10 nM insulin for 2 hours.
  • Cells are harvested, mRNA extracted using magnetic DYNAL beads and reverse transcribed to cDNA using Superscript First-Strand Synthesis as described by the manufacturer. Appropriate primers are designed and used in 15 uL PCR reaction using 55 deg annealing temperature and 30 cycles of amplification.
  • C2C12 cells (murine skeletal muscle cell line; ATCC CRL 1772, Rockville, MD) are seeded sparsely (about 15-20%) in complete DMEM (w/glutamine, pen/strep, etc) + 10% FCS. Two days later they become 80-90% confluent. At this time, the media is changed to DMEM+2% horse serum to allow differentiation. The media is changed daily. Abundant myotube formation occurs after 3-4 days of being in 2% horse serum, although the exact time course of C2C12 differentiation depends on how long they have been passaged and how they have been maintained, among other factors.
  • test molecules e.g., test peptides added in a range of 1 to 2.5 ⁇ g/mL
  • test molecules are added the day after seeding when the cells are still in DMEM with 10% FCS.
  • Two days after plating the cells one day after the test molecule was first added, at about 80-90% confluency, the media is changed to DMEM+2% horse serum plus the test molecule.
  • C2C12 cells are differentiated in the presence or absence of 2 ⁇ g/mL test molecules for 4 days. On day 4, oleate oxidation rates are determined by measuring conversion of l- 14 C-oleate (0.2 mM) to 14 CO 2 for 90 min. This experiment can be used to screen for active polypeptides and peptides as well as agonists and antagonists or activators and inhibitors of SLC22Al, TTN 1 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLB polypeptides or binding partners.
  • test molecules on the rate of oleate oxidation can be compared in differentiated C2C12 cells (murine skeletal muscle cells; ATCC, Manassas, VA CRL-1772) and in a hepatocyte cell line (Hepal-6; ATCC, Manassas, VA CRL-1830). Cultured cells are maintained according to manufacturer's instructions.
  • the oleate oxidation assay is performed as previously described (Muoio et al (1999) Biochem J 338;783-791). Briefly, nearly confluent myocytes are kept in low serum differentiation media (DMEM, 2.5% Horse serum) for 4 days, at which time formation of myotubes becomes maximal.
  • DMEM low serum differentiation media
  • Hepatocytes are kept in the same DMEM medium supplemented with 10% FCS for 2 days. One hour prior to the experiment the media is removed and 1 mL of preincubation media (MEM, 2.5% Horse serum, 3 mM glucose, 4 mM Glutamine, 25 mM Hepes, 1% FFA free BSA, 0.25 mM Oleate, 5 ⁇ g/mL gentamycin) is added.
  • preincubation media MEM, 2.5% Horse serum, 3 mM glucose, 4 mM Glutamine, 25 mM Hepes, 1% FFA free BSA, 0.25 mM Oleate, 5 ⁇ g/mL gentamycin
  • test molecule e.g., 2.5 ⁇ g/mL of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GL/3-related test peptide.
  • test molecule e.g., 2.5 ⁇ g/mL of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GL/3-related test peptide.
  • 0.75 mL of the media is removed and assayed for 14 C-oxidation products as described below for the muscle FFA oxidation experiment.
  • Triglyceride and Protein Analysis, following Oleate Oxidation in Cultured Cells [0347] Following transfer of media for oleate oxidation assay, cells are placed on ice. To determine triglyceride and protein content, ceils are washed with 1 mL of Ix PBS to remove residual media. To each well 300 ⁇ L of cell dissociation solution (Sigma) is added and incubated at 37 0 C for 10 min. Plates are tapped to loosen cells, and 0.5 mL of Ix PBS is added. The cell suspension is transferred to an Eppendorf tube, each well is rinsed with an additional 0.5 mL of Ix PBS, and is transferred to the appropriate Eppendorf tube.
  • Samples are centrifuged at 1000 rpm for 10 minutes at room temperature. Each supernatant is discarded and 750 ⁇ L of Ix PBS/2% CHAPS is added to cell pellet. The cell suspension is vortexed and placed on ice for 1 hour. Samples are then centrifuged at 13000 rpm for 20 min at 4 0 C. Each supernatant is transferred to a new tube and frozen at -2O 0 C until analyzed. Quantitative measure of triglyceride level in each sample is determined using Sigma Diagnostics GPO-TRINDER enzymatic kit.
  • the assay is performed in 48 well plate, 350 ⁇ L of sample volume is assayed, a control blank consists of 350 ⁇ L PBS/2% CHAPS, and a standard contains 10 ⁇ L standard provide in the kit with 690 ⁇ L PBS/2% CHAPS.
  • Analysis of samples is carried out on a Packard Spectra Count at a wavelength of 550 nm.
  • Protein analysis is carried out on 25 ⁇ L of each supernatant sample using the BCA protein assay (Pierce) following manufacturer's instructions. Analysis of samples is carried out on a Packard Spectra Count at a wavelength of 550 nm. Stimulation of insulin secretion in HIT-Tl 5 cells
  • HIT-T 15 is an immortalized hamster insulin-producing cell line. It is known that stimulation of cAMP in HIT-T 15 cells causes an increase in insulin secretion when the glucose concentration in the culture media is changed from 3mM to 15 mM. Thus, test molecules also are tested for their ability to stimulate glucose-dependent insulin secretion (GSIS) in HIT-T15 cells. In this assay, 30,000 cells/well in a 12-well plate are incubated in culture media containing 3 mM glucose and no serum for 2 hours. The media is then changed, wells receive media containing either 3 mM or 15 mM glucose, and in both cases the media contains either vehicle (DMSO) or test molecule at a concentration of interest.
  • DMSO vehicle
  • Some wells receive media containing 1 micromolar forskolin as. a positive control. AU conditions are tested in triplicate. Cells are incubated for 30 minutes, and the amount of insulin secreted into the media is determined by ELISA, using a kit from either Peninsula Laboratories (Cat # ELIS-7536) or Crystal Chem Inc. (Cat # 90060).
  • IEQ islet equivalents
  • strainers Move strainers to next wells (Low 1) with 4 or 5 ml low glucose KRB. Incubate at 37° C for 30 minutes. Collect supernatants into low-binding polypropylene tubes pre- labelled for identification and keep cold.
  • Insulin determinations are performed as above, or by Linco Labs as a custom service, using a rat insulin RIA (Cat. # RI-13K).
  • mice Following is a representative rodent model for identifying thereapeutics for treating human diabetes. Experiments are performed using approximately 6 week old C57B1/6 mice (8 per group). All mice are housed individually. The mice are maintained on a high fat diet throughout each experiment.
  • the high fat diet (cafeteria diet; D12331 from Research Diets, Inc.) has the following composition: protein kcal% 16, sucrose kcal% 26, and fat kcal% 58.
  • the fat is primarily composed of coconut oil, hydrogenated.
  • mice After the mice are fed a high fat diet for 6 days, micro-osmotic pumps are inserted using isoflurane anesthesia, and are used to provide test molecule, saline, and a control molecule (e.g., an irrelevant peptide) to the mice subcutaneously (s.c.) for 18 days.
  • a control molecule e.g., an irrelevant peptide
  • JAMS an d GZ/5-related test peptides are provided at doses of 100, 50, 25, and 2.5 ⁇ g/day and an irrelevant peptide is provided at 10 ⁇ g/day.
  • Body weight is measured on the first, third and fifth day of the high fat diet, and then daily after the start of treatment. Final blood samples are taken by cardiac puncture and are used to determine triglyceride (TG), total cholesterol (TC), glucose, leptin, and insulin levels. The amount of food consumed per day is also determined for each group.
  • mice are administered orally with dextrose at 5 g/kg dose.
  • Test molecule is delivered orally via a gavage needle (p.o. volume at 100 ml).
  • Control Ex-4 is delivered intraperitoneally.
  • Levels of blood glucose are determined at regular time points using Glucometer Elite XL (Bayer).
  • Reduction in blood glucose at each time point may be expressed as percentage of original glucose levels, averaged from the number of animals for each group. Results show the effect SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3-related test peptides and test molecules for improving glucose homeostasis in diabetic animals.
  • test molecule is injected i.p. in 100 ⁇ L saline (e.g., 25 ⁇ g of test peptide).
  • saline e.g. 25 ⁇ g of test peptide
  • the same dose 25 ⁇ g/mL in lOO ⁇ L
  • Control animals are injected with saline (3xl00 ⁇ L). Untreated and treated animals are handled in an alternating mode.
  • Plasma samples are taken in hourly intervals, and are immediately put on ice. Plasma is prepared by centrifugation following each time point. Plasma is kept at -20 0 C and free fatty acids (FFA), triglycerides (TG) and glucose are determined within 24 hours using standard test kits (Sigma and Wako). Due to the limited amount of plasma available, glucose is determined in duplicate using pooled samples. For each time point, equal volumes of plasma from all 8 animals per treatment group are pooled.
  • FFA free fatty acids
  • TG triglycerides
  • glucose is determined in duplicate using pooled samples. For each time point, equal volumes of plasma from all 8 animals per treatment group are pooled.
  • a test molecule i.p. in lOO ⁇ L saline (e.g., 25 ⁇ g of test peptide).
  • test molecule e.g. 50 ⁇ g of test peptide
  • control animals are injected with saline (e.g., 3xl00 ⁇ L). Untreated and treated animals are handled in an alternating mode.
  • Plasma samples are immediately put on ice. Plasma is prepared by centrifugation following each time point. Plasma is kept at -20 0 C and free fatty acids (FFA), triglycerides (TG) and glucose are determined within 24 hours using standard test kits (Sigma and Wako).
  • FFA free fatty acids
  • TG triglycerides
  • glucose are determined within 24 hours using standard test kits (Sigma and Wako).
  • mice are injected with epinephrine.
  • epinephrine Two groups of mice are given epinephrine (5 ⁇ g) by intraperitoneal injection. A treated group is injected with a test molecule (e.g., 25 ⁇ g of test peptide) one hour before and again together with epinephrine, while control animals receive saline. Plasma is isolated and free fatty acids and glucose are measured as described above.
  • Muscles are rinsed for 30 min in incubation media with oxygenation. The muscles are then transferred to fresh media (1.5 mL) and incubated at 30 0 C in the presence of l ⁇ Ci/mL [1- 14 C] oleic acid (American Radiolabeled Chemicals). The incubation vials containing this media are sealed with a rubber septum from which a center well carrying a piece of Whatman paper (1.5 cm x 11.5 cm) is suspended.
  • test molecules are added to the media (e.g., a final concentration of 2.5 ⁇ g/mL of test peptide) and maintained in the media throughout the procedure.
  • media e.g., a final concentration of 2.5 ⁇ g/mL of test peptide
  • mice are intravenously (tail vein) injected with 30 ⁇ L bolus of Intralipid-20% (Clintec) to generate a sudden rise in plasma FFAs, thus by-passing intestinal absorption.
  • Intralipid is an intravenous fat emulsion used in nutritional therapy.
  • a treated group (treated with test molecule) is injected with a test molecule (e.g., 25 ⁇ g of a test peptide) at 30 and 60 minutes before Intralipid is given, while control animals receive saline. Plasma is isolated and FFAs are measured as described previously. The effect of a test molecule on the decay in plasma FFAs following the peak induced by Intralipid injection is then monitored.
  • the db/db mice progressively develop insulinopenia with age, a feature commonly observed in late stages of human type II diabetes when blood sugar levels are insufficiently controlled.
  • the state of the pancreas and its course vary according to the models. Since this is a model of type II diabetes mellitus, test molecules are tested for blood sugar and triglycerides lowering activities.
  • Zucker (fa/fa) rats are severely obese, hyperinsulinemic, and insulin resistant (Coleman, Diabetes 31 :1, 1982; E. Shafrir, in Diabetes Mellitus; H. Rifkin and D. Porte, Jr. Eds. (Elsevier Science Publishing Co., Inc., New York, ed. 4, 1990), pp.
  • the fa/fa mutation may be the rat equivalent of the murine db mutation (Friedman et al., Cell 69:217- 220, 1992; Truett et al., Proc. Natl. Acad. Sci. USA 88:7806, 1991).
  • Tubby tub/tub>mice are characterized by obesity, moderate insulin resistance and hyperinsulinemia without significant hyperglycemia (Coleman et al., J. Heredity 81:424, 1990).
  • leptin is reported to reverse insulin resistance and diabetes mellitus in mice with congenital lipodystrophy (Shimomura et al. Nature 401 : 73-76 (1999). Leptin is found to be less effective in a different lipodystrophic rodent model of lipoatrophic diabetes (Grajova et al Nature 403: 850 (2000); hereby incorporated herein in its entirety including any drawings, figures, or tables).
  • STZ streptozotocin
  • the monosodium glutamate (MSG) model for chemically- induced obesity (Olney, Science 164:719, 1969; Cameron et al., Clin Exp Pharmacol Physiol 5:41, 1978), in which obesity is less severe than in the genetic models and develops without hyperphagia, hyperinsulinemia and insulin resistance, is also examined.
  • a non-chemical, non-genetic model for induction of obesity includes feeding rodents a high fat/high carbohydrate (cafeteria diet) diet ad libitum.
  • Test molecules are tested for reducing hyperglycemia in any or all of the above rodent diabetes models or in humans with type II diabetes or other metabolic diseases described previously or models based on other mammals.
  • the test molecule sometimes is combined with another compatible pharmacologically active antidiabetic agent such as insulin, leptin (US provisional application No 60/155,506), or troglitazone, either alone or in combination.
  • test molecules are administered intraperitoneally, subcutaneously, intramuscularly or intravenously. Glucose and insulin levels of the mice are tested, food intake and liver weight monitored, and other factors, such as leptin, FFA, and TG levels, often are measured in these tests.
  • mice Genetically altered obese diabetic mice (db/db) (male, 7-9 weeks old) are housed (7-9 mice/cage) under standard laboratory conditions at 22° C and 50% relative humidity, and maintained on a diet of Purina rodent chow and water ad libitum. Prior to treatment, blood is collected from the tail vein of each animal and blood glucose concentrations are determined using One Touch Basic Glucose Monitor System (Lifescan). Mice that have plasma glucose levels between 250 to 500 mg/dl are used.
  • Each treatment group consists, of seven mice that are distributed so that the mean glucose levels are equivalent in each group at the start of the study, db/db mice are dosed by micro-osmotic pumps, inserted using isofturane anesthesia, to provide test molecules, saline, and an irrelevant peptide to the mice subcutaneously (s.c).
  • Blood is sampled from the tail vein hourly for 4 hours and at 24, 30 h post-dosing and analyzed for blood glucose concentrations.
  • Food is withdrawn from 0-4 h post dosing and reintroduced thereafter.
  • Individual body weights and mean food consumption are also measured after 24 h. Significant differences between groups (comparing test molecule treated to saline-treated) are evaluated using a Student t-test.
  • Tests of the efficacy of test molecules in humans are performed in accordance with a physician's recommendations and with established guidelines.
  • the parameters tested in mice are also tested in humans ⁇ e.g. food intake, weight, TG, TC, glucose, insulin, leptin, FFA). It is expected that the physiological factors are modified over the short term. Changes in weight gain sometimes require a longer period of time. In addition, diet often is carefully monitored.
  • Test molecules often are administered in daily doses (e.g., about 6 mg test peptide per 70 kg person or about 10 mg per day). Other doses are tested, for instance 1 mg or 5 mg per day up to 20 mg, 50 mg, or 100 mg per day.
  • genomic nucleotide sequence for a SLC22A1 region.
  • the genomic nucleotide sequence is set forth in SEQ ID NO: 1.
  • the following nucleotide representations are used throughout: "A” or “a” is adenosine, adenine, or adenylic acid; “C” or “c” is cytidine, cytosine, or cytidylic acid; “G” or “g” is guanosine, guanine, or guanylic acid; “T” or “t” is thymidine, thymine, or thymidylic acid; and “I” or “i” is inosine, hypoxanthine, or inosinic acid.
  • SNPs are designated by the following convention: “R” represents A or G, “M” represents A or C; “W” represents A or T; “Y” represents C or T; “S” represents C or G; “K” represents G or T; “V” represents A, C or G; “H” represents A, C, or T; “D” represents A, G, or T; “B” represents. C, G, or T; and 'TST" represents A, G, C, or T.

Abstract

Provided herein are methods for identifying a risk of type II diabetes in a subject, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating type II diabetes, and therapeutic and preventative methods applicable to type II diabetes. These embodiments are based upon an analysis of polymorphic variations in nucleotide sequences within the human genome.

Description

METHODS FOR IDENTIFYING RISK OF TYPE H DIABETES AND TREATMENTS THEREOF
Field of the Invention
[0001] The invention relates to genetic methods for identifying predisposition to type II diabetes, also known as non-insulin dependent diabetes, and treatments that specifically target the disease.
Background
[0002] Diabetes is among the most common of all metabolic disorders, affecting up to 11% of the population by age 70. Type I diabetes (insulin-dependent diabetes) represents about 5 to 10% of this group and is the result of progressive autoimmune destruction of the pancreatic β-cells with subsequent insulin deficiency.
[0003] Type II diabetes (non-insulin dependent diabetes) represents 90-95% of the affected population, more than 100 million people worldwide. Approximately 17 million Americans suffer from type II diabetes, although 6 million don't even know they have the disease. The prevalence of the disease has jumped 33% in the last decade and is expected to rise further as the baby boomer generation gets older and more overweight. The global figure of people with diabetes is set to rise to an estimated 150 to 220 million in 2010, and 300 million in 2025. The widespread problem of diabetes has crept up on an unsuspecting health care community and has already imposed a huge burden on health-care systems (Zimmet et al (2001) Nature 414: 782-787).
[0004] Often, the onsebof type II diabetes can be insidious, or even clinically unapparent, making diagnosis difficult. Even when the disease is properly diagnosed, many of those treated do not have adequate control over their diabetes, resulting in elevated sugar levels in the bloodstream that slowly destroys the kidneys, eyes, blood vessels and nerves. This late damage is an important factor contributing to mortality in diabetics.
[0005] Type II diabetes is associated with peripheral insulin resistance, elevated hepatic glucose production, and inappropriate insulin secretion (DeFronzo, R. A. (1988) Diabetes 37:667- 687), although the primary pathogenic lesion on type II diabetes remains elusive. Many have suggested that primary insulin resistance of the peripheral tissues is the initial event. Genetic epidemiological studies have supported this view. Similarly, insulin secretion abnormalities have been argued as the primary defect in type II diabetes. It is likely that both phenomena are important in the development of type II diabetes, and genetic defects predisposing to both are likely to be important contributors to the disease process (Rimoin, D.L., et al. (1996) Emery and Rimoin's Principles and Practice of Medical Genetics 3rd Ed. 1: 1401-1402).
[0006] Evidence from familial aggregation and twins studies point to a genetic component in the etiology of diabetes (Newman et al. (1987) Diabetologia 30:763-768; Kobberling, J. (1971) Diabetologia 7:46-49; Cook, J. T. E. (1994) Diabetologia 37:1231-1240), however, there is little agreement as to the nature of the genetic factors involved. This confusion can largely be attributed to the genetic heterogeneity known to exist in diabetes.
Summary
[0007] It has been discovered that certain polymorphic variations in human genomic DNA are associated with the occurrence of type II diabetes, also known as non-insulin dependent diabetes. In particular, polymorphic variants in loci containing SLC22A1, TTN, LOCI 12609 (also known as C6orfll7), TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLLS regions in human genomic DNA have been associated with risk of type II diabetes.
[0008] Thus, featured herein are methods for identifying a subject at risk of type II diabetes and/or a risk of type II diabetes in a subject, which comprise detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in or around loci described herein in a human nucleic acid sample. In an embodiment, two or more polymorphic variations are detected and in some embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variants are detected.
[0009] Also featured are nucleic acids that include one or more polymorphic variations associated with occurrence of type II diabetes, as well as polypeptides encoded by these nucleic acids. In addition, provided are methods for identifying candidate therapeutic molecules for treating type II diabetes and other insulin-related disorders, as well as methods for treating type II diabetes in a subject by identifying a subject at risk of type II diabetes and treating the subject with a suitable prophylactic, treatment or therapeutic molecule.
[0010] Also provided are compositions comprising a cell from a subject having type II diabetes or at risk of type II diabetes and/or a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid, with a RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid designed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a nucleic acid that hybridizes to such a nucleotide sequence under stringent conditions. In an embodiment, the RNAi, siRNA, antisense DNA or RNA, or ribozyme nucleic acid is designed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence that includes one or more type II diabetes associated polymorphic variations, and in some instances, specifically interacts with such a nucleotide sequence. Further, provided are arrays of nucleic acids bound to a solid surface, in which one or more nucleic acid molecules of the array have a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a fragment or substantially identical nucleic acid thereof, or a complementary nucleic acid of the foregoing. Featured also are compositions comprising a cell from a subject having type II diabetes or at risk of type II diabetes and/or a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB polypeptide, with an antibody that specifically binds to the polypeptide. In an embodiment, the antibody specifically binds to an epitope in the polypeptide that includes a non-synonymous amino acid modification associated with type II diabetes (e.g., results in an amino acid substitution in the encoded polypeptide associated with type II diabetes). In an embodiment, the antibody specifically binds to an epitope comprising a glycine corresponding to position 557 in a ZNF221 polypeptide (e.g., a polypeptide having an amino acid sequence in SEQ ID NO: 27).
Brief Description of the Drawings
[0011] Figures 1 A-IC show proximal SNPs in a SLC22A1 region in genomic DNA for females, males and combined results, respectively. Figures 2A-2C show proximal SNPs in a TTN region in genomic DNA for females, males and combined results, respectively. Figures 3A-3C show proximal SNPs in a LOCI 12609 region in genomic DNA for females, males and combined results, respectively. Figures 4A-4C show proximal SNPs in a TRPSl region in genomic DNA for females, males and combined results, respectively. Figures 5A-5C show proximal SNPs in a PRAME region in genomic DNA for females, males and combined results, respectively. Figures 6A-6C show proximal SNPs in a ZNF221 region in genomic DNA for females, males and combined results, respectively. Figures 7A-7C show proximal SNPs in a FOXA2 region in genomic DNA for females, males and combined results, respectively. Figures 8A-8C show proximal SNPs in a JAMS region in genomic DNA for females, males and combined results, respectively. Figures 9A-9C show proximal SNPs in a GLB region in genomic DNA for females, males and combined results, respectively. The position of each SNP in the chromosome is shown on the x-axis and the y-axis provides the negative logarithm of the p-value comparing the estimated allele frequency in the cases to that of the control group. Also shown in the figures are exons and introns of the genes in approximate chromosomal positions.
[0012] Figures 1 OA-I OG show results of an odds-ratio meta analysis for SLC22A1, LOCI 12609, PRAME, ZNF221, FOXA2, JAM3 and GLB regions, respectively. Detailed Description
[0013] It has been discovered that polymorphic variants described in a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB locus in human genomic DNA are associated with occurrence of type II diabetes in subjects. Thus, detecting genetic determinants in and around this locus associated with an increased risk of type II diabetes occurrence can lead to early identification of a risk of type II diabetes and early application of preventative and treatment measures. Associating the polymorphic variants with type II diabetes also has provided new targets for diagnosing type II diabetes, and methods for screening molecules useful in diabetes treatments and diabetes preventatives.
[0014] SLC22A1 encodes solute carrier family 22, member 1, which is a polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs from the systemic circulation, maintaining body fluid homeostasis, and acting as a defense system against toxic agents. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein.
[0015] TTN encodes a 3-megadalton protein composed of 244 copies of immunoglobulin and fibronectin type III domains. TITIN is stringlike in structure and in vivo spans from the M to Z lines, important for structural integrity of the myofibril and passive tension. Titin phosphorylates Titin-cap (a 19 kDa protein) a protein that associates with the N-terminus of titin.
[0016] The trichorhinophalangeal syndrome I (TRPSl) gene has 7 exons and encodes a polypeptide of 1,281 amino acids. The predicted protein sequence has 2 potential nuclear localization signals and an unusual combination of different zinc finger motifs.
[0017] FOXA2 encodes a polypeptide that is a member of a forkhead class of DNA-binding proteins referred to as forkhead box A2. FOXA2 consists of two transcript variants, which differ in the 5' UTR but encode the same protein. FOXA2 serves as a transcription factor involved in the regulation of pancreatic development. Its expression is known to be regulated by HNF6 in human hepatoma cells.
[0018] The JAM3 gene encodes a protein that is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. Tight junctions represent one mode of cell- to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.
[0019] By genomic sequence analysis, Arrate et al. (Chem. 276: 45826-45832, 2001) determined that the JAM3 gene contains 9 exons and spans more than 88 kb. The deduced 310- arnino acid protein is more than 30% identical to JAM2 and JAMl . It possesses a signal sequence; 2 Ig-like folds, one a V type and the other a C2 type, containing 6 cysteines; 2 potential N- glycosylation sites; and a 46-amino acid intracellular tail with a C-terminal binding motif for PDZ domains and a phosphorylation site. Northern blot analysis revealed wide expression of an approximately 3.3-kb transcript, with highest levels in placenta, brain, and kidney. Expression was also detected in cultured endothelial cells.
[0020] The GLB gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the GIi family. They are characterized as a transcription factor that binds to DNA through zinc finger motifs. These motifs have conserved H-C links. GIi family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial Polydactyly type IV, postaxial Polydactyly types Al and B.
Type II Diabetes and Sample Selection
[0021] The term "type II diabetes" as used herein refers to non-insulin-dependent diabetes. Type II diabetes refers to an insulin-related disorder in which there is a relative disparity between endogenous insulin production and insulin requirements, leading to elevated hepatic glucose production, elevated blood glucose levels, inappropriate insulin secretion, and peripheral insulin resistance. Type II diabetes has been regarded as a relatively distinct disease entity, but type II diabetes is often a manifestation of a much broader underlying disorder (Zimmet et al (2001) Nature 414: 782-787), which may include metabolic syndrome (syndrome X), diabetes (e.g., type I diabetes, type II diabetes, gestational diabetes, autoimmune diabetes), hyperinsulinemia, hyperglycemia, impaired glucose tolerance (IGT), hypoglycemia, B-cell failure, insulin resistance, dyslipidemias, atheroma, insulinoma, hypertension, hypercoagulability, microalbuminuria, and obesity and obesity-related disorders such as visceral obesity, central fat, obesity-related type II diabetes, obesity-related atherosclerosis, heart disease, obesity-related insulin resistance, obesity- related hypertension, microangiopathic lesions resulting from obesity-related type II diabetes, ocular lesions caused by microangiopathy in obese individuals with obesity-related type II diabetes, and renal lesions caused by microangiopathy in obese individuals with obesity-related type II diabetes.
[0022] Some of the more common adult onset diabetes symptoms include fatigue, excessive thirst, frequent urination, blurred vision, a high rate of infections, wounds that heal slowly, mood changes and sexual problems. Despite these known symptoms, the onset of type II diabetes is often not discovered by health care professionals until the disease is well developed. Once identified, type II diabetes can be recognized in a patient by measuring fasting plasma glucose levels and/or casual plasma glucose levels, measuring fasting plasma insulin levels and/or casual plasma insulin levels, or administering oral glucose tolerance tests or hyperinsulinemic euglycemic clamp tests.
[0023] Based in part upon selection criteria set forth above, individuals having type II diabetes can be selected for genetic studies. Also, individuals having no history of metabolic disorders, particularly type II diabetes, often are selected for genetic studies as controls. The individuals selected for each pool of case and controls, were chosen following strict selection criteria in order to make the pools as homogenous as possible. Selection criteria for the study described herein included patient age, ethnicity, BMI, GAD (Glutamic Acid Decarboxylase) antibody concentration, and HbAIc (glycosylated hemoglobin AIc) concentration. GAD antibody is present in association ■ with islet cell destruction, and therefore can be utilized to differentiate insulin dependent diabetes (type I diabetes) from non-insulin dependent diabetes (type II diabetes). HbAIc levels will reveal the average blood glucose over a period of 2-3 months or more specifically, over the life span of a red blood cell, by recording the number of glucose molecules attached to hemoglobin.
Polymorphic Variants Associated with Type II Diabetes
[0024] A genetic analysis provided herein linked type II diabetes with polymorphic variant nucleic acid sequences in the human genome. As used herein, the term "polymorphic site" refers to a region in a nucleic acid at which two or more alternative nucleotide sequences are observed in a significant number of nucleic acid samples from a population of individuals. A polymorphic site may be a nucleotide sequence of two or more nucleotides, an inserted nucleotide or nucleotide sequence, a deleted nucleotide or nucleotide sequence, or a microsatellite, for example. A polymorphic site that is two or more nucleotides in length may be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, 20 or more, 30 or more, 50 or more, 75 or more, 100 or more, 500 or more, or about 1000 nucleotides in length, where all or some of the nucleotide sequences differ within the region. A polymorphic site is often one nucleotide in length, which is referred to herein as a "single nucleotide polymorphism" or a "SNP."
[0025] Where there are two, three, or four alternative nucleotide sequences at a polymorphic site, each nucleotide sequence is referred to as a "polymorphic variant" or "nucleic acid variant." Where two polymorphic variants exist, for example, the polymorphic variant represented in a minority of samples from a population is sometimes referred to as a "minor allele" and the polymorphic variant that is more prevalently represented is sometimes referred to as a "major allele." Many organisms possess a copy of each chromosome (e.g., humans), and those individuals who possess two major alleles or two minor alleles are often referred to as being "homozygous" with respect to the polymorphism, and those individuals who possess one major allele and one minor allele are normally referred to as being "heterozygous" with respect to the polymorphism. Individuals who are homozygous with respect to one allele are sometimes predisposed to a different phenotype as compared to individuals who are heterozygous or homozygous with respect to another allele.
[0026] In genetic analysis that associate polymorphic variants with type II diabetes, samples from individuals having type II diabetes and individuals not having type II diabetes often are allelotyped and/or genotyped. The term "allelotype" as used herein refers to a process for determining the allele frequency for a polymorphic variant in pooled DNA samples from cases and controls. By pooling DNA from each group, an allele frequency for each SNP in each group is calculated. These allele frequencies are then compared to one another. The term "genotyped" as used herein refers to a process for determining a genotype of one or more individuals, where a "genotype" is a representation of one or more polymorphic variants in a population.
[0027] A genotype or polymorphic variant may be expressed in terms of a "haplotype," which as used herein refers to two or more polymorphic variants occurring within genomic DNA in a group of individuals within a population. For example, two SNPs may exist within a gene where each SNP position includes a cytosine variation and an adenine variation. Certain individuals in a population may carry one allele (heterozygous) or two alleles (homozygous) having the gene with a cytosine at each SNP position. As the two cytosines corresponding to each SNP in the gene travel together on one or both alleles in these individuals, the individuals can be characterized as having a cytosine/cytosine haplotype with respect to the two SNPs in the gene.
[0028] As used herein, the term "phenotype" refers to a trait which can be compared between individuals, such as presence or absence of a condition, a visually observable difference in appearance between individuals, metabolic variations, physiological variations, variations in the function of biological molecules, and the like. An example of a phenotype is occurrence of type II diabetes.
[0029] Researchers sometimes report a polymorphic variant in a database without determining whether the variant is represented in a significant fraction of a population. Because a subset of these reported polymorphic variants are not represented in a statistically significant portion of the population, some of them are sequencing errors and/or not biologically relevant. Thus, it is often not known whether a reported polymorphic variant is statistically significant or biologically relevant until the presence of the variant is detected in a population of individuals and the frequency of the variant is determined. Methods for detecting a polymorphic variant in a population are described herein, specifically in Example 2. A polymorphic variant is statistically significant and often biologically relevant if it is represented in 5% or more of a population, sometimes 10% or more, 15% or more, or 20% or more of a population, and often 25% or more, 30% or more, 35% or more, 40% or more, 45% or more, or 50% or more of a population.
[0030] A polymorphic variant may be detected on either or both strands of a double-stranded nucleic acid. Also, a polymorphic variant may be located within an intron or exon of a gene or within a portion of a regulatory region such as a promoter, a 5 ' untranslated region (UTR), a 3 ' UTR, and in DNA (e.g., genomic DNA (gDNA) and complementary DNA (cDNA)), RNA (e.g., mRNA, tRNA, and rRNA), or a polypeptide. Polymorphic variations may or may not result in detectable differences in gene expression, polypeptide structure, or polypeptide function.
[0031] It was determined that polymorphic variations associated with an increased risk of type II diabetes existed in SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLI3 nucleotide sequences. In the SLC22A1 locus, polymorphic variants at positions selected from the group consisting of rsl888727, rs998203, rs600324, rs2297366, rs2297367, rs755883, rs755884, rs2297369, rs2297370, rs2297371, rs2297372, rsl050015, rsl479355, rs457543, rs456598, rs2297373, rsl867351, rs461473, rs2777353, rs435593, rs3822841, rs683369, rs3798175, rs3798174, rs3737088, rs697735, rs3777394, rs3822840, rs366049, rs366048, rs594709, rs3798173, rs3798172, rs806383, rs3798170, rs3798169, rs3777393, rs3777392, rs2282142, rs2282143, rsl871388, rs3798168, rs628031, rsl867350, rs662138, rø644992, rs637841, rs3798167, rs2197296, rs619598, rs622342, rs715083, rsl382785, rs650284, rsl443844, rs3798164, rs2297374, rs3798163, rs3798162, rs3818678, rs609468, rsl804945, rs651164, rs2083867, rs3101826, rs2343403, rsl871389, rsl479353, rs959814, rs719631, rs791194, rs705771, rs791195, rs791196, rs3911977, rs791198, rs791174, rs791175, rs791176, rs3861971, rs3894314, rs791179, rs791183, rs791184, rs791185, rs3103346, rs791186, rs791187, rs791188, rs791189 and rs791190 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs456598, rs3798168, rs662138, rs644992, rs637841, rs622342, rs650284 and rs2297374. At these positions in SEQ ID NO:1, a guanine at position 24609, a thymine at position 45495, a cytosine at position 50164, a guanine at position 51691, an adenine at position 53067, an adenine at position 58554, an adenine at position 60223, and a thymine at position 61673 were associated with risk of type II diabetes. [0032] In the TTN locus, polymorphic variants at positions selected from the group consisting of rs2195070, rs2303537, rs967507, rsl063160, rsl047500, rs2366577, rs2288324, rs956514, rs957875, rs3217295, rs2857273, rs2857272, rs2857271, rs3829749, rs3813250, rs3813249, rs2857269, rs3829748, rs2857268, rs3829747, rs3731753, rs2857266, rs3731752, rs2857265, rs962659, rs2278196, rs2857264, rs2303538, rs2303539, rs2288325, rs3820978, rs3731751, rs3731750, rs3731749, rs2555811, rs2555812, rs2555813, rs2555814, rs2555815, rs2857267, rs2555816, rs2555817, rs2288326, rs2555818, rs2555819, rs2288327, rs3731748, rs747122, rs890578, rs3815874, rs3731747, rs2366751, rs3829746, rs3813247, rs3731746, rs3731745, rs3731744, rs3813246, rs3813245, rs3813244, rs3813243, rs3731743, rs744428, rs744426, rs2288572, rs3769864, rs2288571, rs2288570, rs2303838, rs2303837, rs2042996, rsl007025, rsl560221, rs2163009, rs2303836, rs2288569, rs2303835, rs3214578, rs2288568, rsl001238, ^2303834, rs2303833, rs2303832, rs2303831, rs2288567, rs2288566, rs2115559, rs2115558, rs3214577, rs2288565, rs2303830, rsl429095, rs3835071, rsl864243, rs2303829, rs2366752, rslO17323, rs2288563, rs2163010, rs2742324, rs2562843, rsl569407, rs2243452, rs3731741, rs2246932, rs2562844, rs3754951, rsl469473, rsl366677, rs2562845, rs2742356, rs2742355, rs2562846, rsl 429094, rs2742354, rs2163008, rs2115557, rs2562848, rs2562849, rs2742353, rs2562850, rs2742352, rs2252353, rs2252350, rs2472751, rs2742351, rs2742350, rs2742349, rs2303828, rs2288564, rs2627038, rs2244492, rs2042995, rs3045696 and rs2217347 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs2195070, rs967507, rs2366577, rs3829747, rs2857265, rs2303539, rs3731750, rs3731749, rs747122, rs2366751, rs3813247, rs3731745, rs3813246, rs3813245, rs744426, rs2042996, rsl560221, rs2288569, rs2288568, rsl001238, rs2288566, rs2288563, rs2243452, rs2246932, rs2562844, rsl 366677, rs2742355, rs2562846, rs2163008, rs2562848, rs2562849, rs2742353, rs2252353, rs2472751, rs2742350, rs2742349, rs2627038 and rs2042995. At these positions in SEQ E) NO:2, a cytosine at position 244, a guanine at position 1930, a thymine at position 6826, an adenine at position 32405, a thymine at position 33667, an adenine at position 38437, a thymine at position 40847, an adenine at position 41035, a thymine at position 49162, a guanine at position 62030, an adenine at position 64904, a cytosine at position 66438, a guanine at position 68424, a cytosine at position 68983, a thymine at position 70864, a thymine at position 86264, a cytosine at position 89238, an adenine at position 93435, a cytosine at position 99088, a guanine at position 99370, an adenine at position 109309, a cytosine at position 134373, a guanine at position 140739, an adenine at position 142918, a cytosine at position 145347, a thymine at position 149263, a cytosine at position 150280, a cytosine at position 150530, an adenine at position 163221, a thymine at position 163832, a guanine at position 164116, a cytosine at position 164340, a cytosine at position 167905, a thymine at position 174746, a cytosine at position 177001, a thymine at position 177076, a thymine at position 188760 and a guanine at position 193209 were associated with risk of type II diabetes.
[0033] In the LOCI 12609 locus, polymorphic variants at positions selected from the group consisting of rs6936939, rs6941487, rs6941805, rs6941892, rs6899315, rs6942240, rsl928279, rs2497106, rs7754453, rs2497105, rs7775786, rs2480185, rs2480186, rs2497151, rs2480187, rs2480188, rs2480189, rs2497160, rs2497162, rs2497108, rs6937611, rs2497113, rs2497115, rs2480191, rs2497116, rs2497117, rs2497118, rs7776208, rs7756256, rs7756316, rs7756587, rs2480192, rs2497119, rs6906099, rs6926656, rsl928281, rs2225244, rs7773403, rsl556111, rs2497120, rs6913756, rs6918019, rs2497146, rs2497145, rsl928282, rs2480194, rs2480195, rs6936847, rs6914226, rs7383205, rs6933393, rs6922368, rs2480196, rs6928025, rs6928551, rs2497136, rs6928815, rsl810806, rs2497135, rs6934877, rs2497133, rs2497132, rs2497131, rs2480197, rs2875382, rs7450001, rs2497130, rs2497129, rslO45649, rs3219, rs8348, rs3218, rs2497127, rs2497126, rs2480198, rs2480199, rs2497123, rs2497122, rs6902104, rs2497121, rs2480200, rs2480201, rsl928283, rs9449785, rs5877871, rsl080960, rsl 114811, rs4097471, rs4097472, rs3857473, rs2476913, rs2476894, rs2324637, rs9449794, rs7741955, rs2476912, rs6901546, rs3778180, rs6913525, rs2497148, rs6930183, rs6936765, rs7771595, rs7775669, rs2497149, rs2497150, rs728721, rs2476899, and rs2476900 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type π diabetes: rs2480187, rs6926656, rs2497120, rs2480195, rs6914226, rs6922368, rs2480196, rs2497136, rs2497131, rs2480197, rs3219, rs2497126, rs2480201, rs2476913, rs9449794, rs7741955, and rs2497150. At these positions in SEQ ID NO:3, an adenine at position 10416, an adenine at position 23217, a guanine at position 26174, a cytosine at position 31876, a thymine at position 39759, an adenine at position 45065, a guanine at position 45809, a guanine at position 46015, a cytosine at position 48028, a guanine at position 48093, a guanine at position 49824, an adenine at position 50308, a cytosine at position 55145, a cytosine at position 76098, a cytosine at position 78655, an adenine at position 79068, and a cytosine at position 96459 were associated with risk of type II diabetes.
[0034] Li the TRPSl locus, polymorphic variants at positions selected from the group consisting of rs2625681, rs2625680, rs2625678, rs2625676, rs2737202, rsl905376, rs2178946, rs800919, rs2142327, rs2884365, rs800918, rs800917, rs800916, rs2625683, rs2625682, rsl483583, rs3808432, rsl483584, rsl483585, rs800912, rs3779878, rs3808433, rs3808434, rs3808435, rs3808436, rs3808437, rs3824201, rs3808438, rs3808439, rs3808440, rsl351744, rsl483589, rs3802219, rs2884366, rs4633093, rs2358019, rsl872075, rs3824202, rs3824203, rs3808444, rs3802221, rs3779879, rs3779880, rs3808447, rs3808448, rs3808449, rs3808450, rs3808451, rs2223103, rsll60333, rs3808452, rslO28274, rsl963677, rsl963678, rs3779881, rs2358020, rs2358021, rs2049864, rs2049866, rs3808456, rs4876606, rs4876607, rs3808457, rs4876343, rs4876608, rs3779882, rs719422, rs2178949, rs2272619, rslO57517, rs3808460, rs3808461, rs4141424, rs2293889, rs3802222, rs2737203, rs2721954, rs2721968, rs2721969, rs3808462, rs2049869, rs2721927, rs2737205, rs2721928, rs2721929, rs3779883, rs4876609, rs2737207, rs2737208, rs2737210, rs2737211, rs2721930, rs2244644, rs2721932, rs2245019, rs2976473, rs2737213, rs2245221,,rs2737214, rs2721933, rs2721935, rs2737216, and rs2737217 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rsl905376, rs2178946, rsl483583, rs3779878, rs3808435, rs3808437, rs3808438, rs3808440, rsl483589, rs3802219, rs2884366, rs3779879, rs3808447, rs3808448, rs3808450, rs2223103, rsl 160333, rslO28274, rsl963677, rs3779881, rs2358021, rs2049864, rs2049866, rs3808456, rs3808457,,rs719422, rs2178949, rs2272619, rs3808461, rs2737203, rs2737205, rs2721929, rs2737207, rs2721932, rs2245019, rs2245221, rs2737214, and rs2737216. At these positions in SEQ ID NO:4, a thymine at position 2040, a guanine at position 5340, an adenine at position 17458, an adenine at position 24581, a guanine at position 28176, a thymine at position 30025, a cytosine at position 31855, a guanine at position 31978, an adenine at position 33078, a thymine at position 33647, a cytosine at position 34121, a cytosine at position 41305, a guanine at position 43741, a thymine at position'45109, a guanine at position 47330, an adenine at position 47601, a thymine at position 49006, a guanine at position 51833, a guanine at position 52151, a cytosine at position 54742, a guanine at position 55740, a cytosine at position 56608, a thymine at position 56831, a guanine at position 57500, a thymine at position 57779, a thymine at position 58542, a thymine at position 62420, a guanine at position 65321, a cytosine at position 65998, a thymine at position 71285, a thymine at position 78462, a guanine at position 81540, a cytosine at position 82825, a guanine at position 88586, a cytosine at position 91188, a guanine at position 93161, a cytosine at position 93269, and an adenine at position 98361 were associated with risk of type II diabetes.
[0035] In the PRAME locus, polymorphic variants at positions selected from the group consisting of rsl 1109, rs361737, AA at position 522, rs2236729, rs2236730, rs2051486, rs2051487, rs2051488, rs965764, rs2006783, rs2032416, rs362139, AB at position 19890, rs361901, rs361676, rs362184, rs361797, rs361907, rs362004, rs362176, rs361570, rs361595, rs361778, rs361709, rs361558, rs361762, rs361737, rs362003, rs362011, rs361721, rs361959, rs362230, rs361970, rs362012, rs361671, rs361876, rs362168, rs361830, rs362257, rs362167, rs362015, rs361976, rs362044, rs362245, rs361986, rs362241, rs361582, rs362208, rs361755, rs361864, rs362107, rs361535, rs361940, rs361828, rsll090131, rs361894, rs361829, rs2156888, rs2156889, rs2156890, rs738887, rs738888, rs3827305, rs3747098, rs8405, rs7104, rsl3604, rs2229696, rs2072049, rslO24372, rslO24373, rs2073725, rs2072050, rs2229695, rs739463, rs3216404, rs2266981, rs2266982, rs2266983, rs2266984, rs2266985, rs2266988, rs2283798, rs2283799, rs2283800, rs2073916, rs2330188, rs2877079, rs2330189, rs424233, rs424248, rs2330190, rs2266991, rs2266992, rs3827306, rs3827307, rs2859957, rs737891, rs2097206, rs363968, rs2078843, rs2330191, rs2330192, rs2877080, rs715594, rs715591, rs715587, rs715593, rs459197, rs467133, rs723182, rs460606, rs457907, rs460608, rs457967, rsl807539, rs464317, rs459939, rs466341, rs2156893, rs9306380, rs2156895, rs2187589, rs429560, rs2236731, rs2236732, rs392546, rs2236733, rsl557617, rsl557618, rs715516, rs741673 and rs2330193 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rsl 1109, rs361709, rs362012, rs362167, rs361976, rs361864, rs362107, rs361940, rs361828, rs361829, rs2156888, rs2156889, rs3827305, rs7104, rsl3604, rslO24372, rs2283799, rs2877079, rs2266991, rs3827306, rs460608, rs457967, rs2236732 and rs715516. At these positions in SEQ ID NO:5, a thymine at position 210, a thymine at position 26132, an adenine at position 29161, a guanine at position 30821, a thymine at position 31642, a cytosine at position 33794, a thymine at position 33946, a thymine at position 34259, a thymine at position 34390, a thymine at position 35021, a thymine at position 36974, an adenine at position 36976, an adenine at position 47958, an adenine at position 48306, a cytosine at position 48496, an adenine at position 49199, a guanine at position 57368, a thymine at position 60256, a cytosine at position 60956, a guanine at position 61792, a cytosine at position 79592, a guanine at position 79694, a guanine at position 89193, and a thymine at position 94819 were associated with risk of type II diabetes.
[0036] In the ZNF221 locus, polymorphic variants at positions selected from the group consisting of rs374652, rs450308, rs424729, rs423752, rs375066, rs384329, rs424410, rs396063, rs398388, rs404547, rs413093, rs379785, rs428505, rs454559, rs367741, rs444350, rs373168, rs368316, rs411803, rs419990, rs445865, rs386747, rs386729, rs454376, rs453682, rs452143, rs444816, rs377931, rs365769, rs379722, rs2136302, rs396188, rs424041, rs386249, rs385202, rs394380, rs388547, rs442518, rs2356537, rs6509123, rs2356538, rs424673, rs407375, rs431230, rsl703747, rs390134, rs415468, rs367241, rs2284243, rs414122, rs446072, rs47775, rs373043, rs438174, rs429611, rs714420, rs442543, rs2356543, rs391596, rs441386, rs407806, rs381872, rs393473, rs393403, rs393117, rs413061, rs383133, rs397276, rs369958, rs377174, rs451601, rs451612, rs453932, rs439676, rs453640, rs365745, rs366111, rs376769, rs447175, rs457642, rs2535490, rs461374, rs465860, rs385016, rs364378, rs2191565, rs382715, rs397133, rs397706, rs390784, rs398981, rs425530, rs415499, rs415521, rs384596, rs431166, rs436790, rs392889, rs391267, rs414402, rs381888, rs393195, rs405763, rs370809, rs397964, rs397683, rs382255, rs381983, rs396497, rs396148, rs396116, rs395803, rs448692, rs381759, rs420584, rs381091, rs420355, rs436739, rs419839, rs431509, rs421505, rs403296, rs393138, rs415913, rs2302411, rs446016, rs434585, rs452554, rs449664, rs367499, rs384544, rs369083, rs380726, rs379238, rs367209, rs2270196, rs369106, rs390369, rs451388, rs430107, rs2356549, rs2191566, rs454904, rs427608, rsl060877, rslO6O88O, and rsl2753 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs374652, rs445865, rs2356537, rs2356538, rs2284243, rs442543, rs397276, rs365745, rs366111, rs2191565, rs381888, rs415913, rs451388, rs430107 and rs2191566. At these positions in SEQ ID NO:6, a guanine at position 209, a thymine at position 13440, a guanine at position 26584, a thymine at position 27428, a guanine at position 37614, a thymine at position 42857, a cytosine at position 45945, a thymine at position 49749, a guanine at position 49863, a thymine at position 63533, an adenine at position 71545, a thymine at position 77130, a guanine at position 85661, a guanine at position 89238, and an adenine at position 89929 were associated with risk of type π diabetes.
[0037] In the FOXA2 locus, polymorphic variants at positions selected from the group consisting of rsl415817, rs952991, rs2404166, rs2896772, rs2404167, rsl011104, rsl572580, rsl999861, rsl999862, rsl999863, rsl572581, rsl890206, rsl890207, rsl890208, rsl415818, rs952690, rsl890210, rsl933206, rs2021680, rs2021681, rsl203866, rsl203867, rsl203868, rsl203869, rs945982, rsl203870, rsl203871, rsl203872, rsl203873, rsl203874, rsl203876, rsl203877, rsl203879, rsl203880, rsl203881, rslO44293, rsl203883, rsl203884, rsl203886, rs2471, rsl203888, rsl203889, rsl337925, rsl337926, rsl203890, rsl203891, rs2277762, rs2277763, rsl203892, rs932560, rsl203893, rsl203894, rsl203895, rsl203896, rsl203897, rsl203898, rsl203899, rsl203901, rsl203903, rsl203904, rsl203905, rsl203906) rsl203907, rsl203908, rsl203909, rsll482531, rsl055080, rsl212275, rsl203911, rs2277764, rsl203912, rsl337918, rsl209523, rsl337919, rs2145062, rsl040754, rsl203913, rsl337920, rsl337921, rsl203914, rsl203916, rsl203917, rs910958, rs910959, rs2003076, rsl203919, rsl203920, rsl203921, rsl203922, rsl203923, rsl203924, rsl007458, rs995734, rsl203925, rsl007202, rsl203928, rsl203929, rsl203930, rsl203931, rsl203932, and rsl203933 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs2404167, rs2021681, rsl203868, rs945982, rsl203871, rsl203873, rsl203876, rsl203877, rsl203879, rsl203880, rsl203881, rsl203883, rsl203884, rsl203886, rs2471, rsl203889, rs2277763, rsl203892, rs932560, rsl203894, rsl203896, rsl203898, rsl203899, rsl203905, rsl203907, rsl 1482531, rsl055080, rs2277764, rsl337918, rsl209523, rsl337919, rsl337920, rsl337921, rsl203916, rsl203917, rs910958, rs910959, rsl203919 and rs995734. At these positions in SEQ ED NO:7, a guanine at position 17033, a guanine at position 36359, an adenine at position 39612, a guanine at position 41741, an adenine at position 43280, a cytosine at position 45533, a cytosine at position 48515, a guanine at position 49076, a cytosine at position 49416, a cytosine at position 49623, a cytosine at position 50121, a cytosine at position 51246, a guanine at position 51300, a thymine at position 52115 , a cytosine at position 52349, a cytosine at position 54521, a thymine at position 55894, a guanine at position 56120, a guanine at position 56298, a cytosine at position 56969, a guanine at position 58694, a cytosine at position 60740, a thymine at position 60896, a guanine at position 68289, a cytosine at position 68571, a guanine at position 69926, an adenine at position 70038, a cytosine at position 73724, an adenine at position 75208, a thymine at position 75542, a thymine at position 76195, a thymine at position 78804, a guanine at position 78808, a cytosine at position 83881, a cytosine at position 84115, an adenine at position 85604, a cytosine at position 85611, a thymine at position 90554, and a guanine at position 95608 were associated with risk of type II diabetes.
[0038] In the JAMS locus, polymorphic variants at positions selected from the group consisting of rs470855, rs470780, rs674487, rs5795871, rs2510345, rsl784500, rs484233, rs470755, rs470796, rs643141, rs631156, rs630723, rs7125310, rs470982, rs599808, rs470437, rs470434, rs470372, rs470474, rs678161, rs470536, rs676606, rs470461, rs7123926, rs470522, rs470388, rsl892895, rsll43810, rsll43812, rs470639, rs470854, rs470565, rs596004, rs596445, rsl271678, rs470960, rs470953, rs639165, rs626717, rs658544, rs621953, rs503044, rs470882, rs470966, rs470425, rs470957, rs610684, rs623823, rs7121086, rs7942767, rs625740, rs624504, rs470829, rs7933094, rs7946580, rs470631, rs470547, rs618700, rs470500, rs595196, rs470846, rslO51O36, rs7101690, rs470952, rs4077351, rs4937867, rs470719, rs470713, rs470407, rs470659, rs470570, rs2155088, rs470452, rs470564, rsl 144241, rsl939124, rs470680, rs4264164, rsl783814, rsl271129, rs7947580, rs470936, rs470459, rs470600, rs6590734, rs470608, rs2276266, rs4937868, rs646998, rs560925, rs470809, rs532421, rsl815858, rs627592, rs646513, rs694186, rs595584, rs610382, rs610829, rs7945622, rs597320, rs7936421, rs3839957, rs641024, rs3824994, rs643447, rs655627, rs6590735, rs866908, rs94499, and rs2014708 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs470780, rs2510345, rsl784500, rs470372, rs470474, rs470536, rs470522, rs470639, rs470565, rs470953, rs639165, rs626717, rs658544, rs470882, rs470425, rs610684, rs625740, rs624504, rs470547, rs470500, rs470713, rs470570, rs470936, rs470600, rs470809, rs595584, rs610382, rs597320, and rs2014708. At these positions in SEQ ID NO:8, a guanine at position 230, a thymine at position 1650, a thymine at position 1798, a cytosine at position 9389, a cytosine at position 9422, a guanine at position 10067, a thymine at position 11304, a guanine at position 17202, a cytosine at position 17685, an adenine at position 18562, a thymine at position 21257, a thymine at position 21487, a guanine at position 22672, a thymine at position 24679, an adenine at position 25380, a thymine at position 26819, a thymine at position 28631, a cytosine at position 28864, a thymine at position 31008, a thymine at position 31157, a cytosine at position 37750, a guanine at position 40366, an adenine at position 48530, a guanine at position 48963, a thymine at position 51765, an adenine at position 57513, a cytosine at position 58508, an adenine at position 59213 and a thymine at position 63711 were associated with risk of type II diabetes.
[0039] In the GLB locus, polymorphic variants at positions selected from the group consisting of rs846323, rs846322, rs846321, rs846320, rs7811555, rs699490, rs846319, rs846318, rs846317, rs846316, rs699491, rs699492, rs699493, rs2840182, rs4724089, rs4724090, rs6953964, rs846269, rs846268, rsl405753, rsl405752, rs2330282, rsl405751, rs846267, rs846266, rs3898405, rs7806570, rs846265, rs3801162, rs846264, rs7785287, rs846263, rs699494, rs2282920, rs7794103, rs4720415, rs4724091, rs2330284, rs2330285, rsl527500, rs917230, rs917229, rs2237423, rs2237422, rs2237421, rs2237420, rsl527499, rs2237419, rs3030352, rsl527498, rs3801164, rs3801165, rs2282919, rsl527496, rs6960238, rs6960725, rs7798461, rs7798596, rs6965985, rs7799496, rs7799683, rs7782596, rs6966658, rs7799542, rs7799584, rs6971570, rs6971751, rs6959829, rs2237417, rs6960695, rs7776923, rs6960887, rs7797875, rs6965961, rs6966146, rs7802706, rs7786060, rs7803421, rs7803428, rs7786698, rs7804280, rs7807699, rs4321900, rs2237416, rs7787368, rs4430004, rs6954947, rs2237415, rs6463087, rs6959294, rs7793034, rs2074741, rsl405750, rs2072201, rs4724092, rs4724093, rs4724094, rs6968300, rs3801166, rs3801167, rs3801168, rslO24552, rslO12136, rslO12135, rs865860, rs866417, rs846298, rs846297, rs846296, rs846295, rs846294, rs846293, rs846291, rs3801170, rs846290, rs2108166, rs3779144, rs846315, rs8463145 rs846313, rs846312, rs8463115 rs846310, rs846309, rs846308, rs846307, rs846306, rs846305, rs2108165, rs846282, rs3779145, rs3801174, rs3779146, rs3779147, rs846280, rs3801176, rs699495, rs3823723, rs2330409, rs2877136, rs2877137, rs3801177, rs 1949974, rs7787414, rs4576336, rs3839731, rs3801178, rsl527501, rs2141174, rs2141175, rs2178029, rs3779149, rs3057241, rs3801180, rsl918380, rsl918381, rs3801181, rs2141176, rs2330412, rs3801182, rs3839733, rsl534316, rs3801183, rs6463088, rs7802174, rs6463089, rs6463090, rs6463091, rs3801184, rs3801185, rs7800024, rs7785042, rs3801186, rs3801187, rs3779152, rs846400, rs3801189, rs846399, rs846398, rs846397, rs846396, rs846395, rs3801192, rs699496, rs699498, rs699499, rs5883813, rs699500, rs3938299, rs3801194, rs846394, rs3801196, rsl405754, rs699502, rs846393, rs3801198, rs846392, rs846391, rs846390, rs846389, rs846388, rs846387, rs846386, rs846385, rs846382, rsl405755, rs846381, rs846380, rs846378, rs6974655, rs846377, rs2049622, rs720790, rs720791, rs720789, and rs5883814 were tested for association with type II diabetes. Polymorphic variants at the following positions were associated with an increased risk of type II diabetes: rs699493, rs846265, rs7785287, rs846263, rs2282920, rs2330284, rs917229, rs2237422, rs2237420, rsl527499, rs6959829, rs2237415, rs6959294, rs7793034, rsl405750, rs4724092, rs4724093, rs3801168, rslO24552, rs846291, rs846290, rs846315, rs846312, rs846311, rs846310, rs846309, rs846308, rs846306, rs2108165, rs3801174, rs3801177, rs3839733, rs3801183, rs6463089, rs3801186 and rs3801192. At these positions in SEQ ID NO:9, a cytosine at position 6109, a guanine at position 9750, a thymine at position 12385, a thymine at position 13672, a thymine at position 17116, a thymine at position 19438, a cytosine at position 20256, an adenine at position 22211, a thymine at position 22773, a thymine at position 23100, a thymine at position 29425, a thymine at position 35962, a guanine at position 36278, a guanine at position 36796, a thymine at position 38135, a cytosine at position 39872, a thymine at position 39897, an adenine at position 40711, a thymine at position 41320, a guanine at position 45949, a thymine at position 46583, a guanine at position 48277, an adenine at position 50176, a cytosine at position 50461, an adenine at position 50703, a guanine at position 51708, a cytosine at position 51981, a cytosine at position 52130, a guanine at position 52582, a guanine at position 56400, an adenine at position 63227, a deletion at position 72440, an adenine at position 72721, an adenine at position 74136, an adenine at position 77539, and a thymine at position 82807 were associated with risk of type II diabetes.
[0040] Based in part upon analyses summarized in Figures IA-I C, 2A-2C, 3A-3C, 4A-4C, 5A-5C, 6A-6C, 7A-7C, 8A-8C and 9A-9C , regions with significant association have been identified in loci associated with type II diabetes. Any polymorphic variants associated with type II diabetes in a region of significant association can be utilized for embodiments described herein. The following reports such regions, where "begin" and "end" designate the boundaries of the region according to chromosome positions within the genomic sequence provided in SEQ ID Nos:l-9. The locus, the chromosome on which the locus resides and an incident polymorphism in the locus also are noted.
COMBINED ASSOCIATION RANGES
Incident SNP Locus Chromosome Begin End Size rs662138 SLC22A1 6 24,609 61,673 37,064 rsl001238 TTN 2 244 193,209 192,965 rs3219 Cόorfl 17 6 10,416 96,459 86,043 rsl 160333 TRPSl 8 2,040 98,361 96,321 rslO24373 PRAME 22 210 94,819 94,609 rs366111 ZNF221 19 209 89,929 89,720 rsl203877 FOXA2 20 17,033 95,608 78,575
«470936 JAM3 11 230 63,711 63,481 rs846312 GLI3 7 6,109 82,807 76,698
Additional Polymorphic Variants Associated with Type II Diabetes
[0041] Also provided is a method for identifying polymorphic variants proximal to an incident, founder polymorphic variant associated with type II diabetes. Thus, featured herein are methods for identifying a polymorphic variation associated with type II diabetes that is proximal to an incident polymorphic variation associated with type II diabetes, which comprises identifying a polymorphic variant proximal to the incident polymorphic variant associated with type II diabetes, where the incident polymorphic variant is in a SLC22A1, TIN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence. The nucleotide sequence often comprises a polynucleotide sequence selected from the group consisting of (a) a polynucleotide sequence of SEQ ID NO: 1-9; (b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence encoded by a polynucleotide sequence of SEQ ID NO: 1-9; and (c) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-9 or a polynucleotide sequence 90% or more identical to the polynucleotide sequence of SEQ ID NO: 1-9. The presence or absence of an association of the proximal polymorphic variant with type II diabetes then is determined using a known association method, such as a method described in the Examples hereafter. In an embodiment, the incident polymorphic variant is a polymorphic variant associated with type II diabetes described herein. In another embodiment, the proximal polymorphic variant identified sometimes is a publicly disclosed polymorphic variant, which for example, sometimes is published in a publicly available database, hi other embodiments, the polymorphic variant identified is not publicly disclosed and is discovered using a known method, including, but not limited to, sequencing a region surrounding the incident polymorphic variant in a group of nucleic samples. Thus, multiple polymorphic variants proximal to an incident polymorphic variant are associated with type II diabetes using this method.
[0042] The proximal polymorphic variant often is identified in a region surrounding the incident polymorphic variant, hi certain embodiments, this surrounding region is about 50 kb flanking the first polymorphic variant (e.g. about 50 kb 5' of the first polymorphic variant and about 50 kb 3' of the first polymorphic variant), and the region sometimes is composed of shorter flanking sequences, such as flanking sequences of about 40 kb, about 30 kb, about 25 kb, about 20 kb, about 15 kb, about 10 kb, about 7 kb, about 5 kb, or about 2 kb 5' and 3' of the incident polymorphic variant, hi other embodiments, the region is composed of longer flanking sequences, such as flanking sequences of about 55 kb, about 60 kb, about 65 kb, about 70 kb, about 75 kb, about 80 kb, about 85 kb, about 90 kb, about 95 kb, or about 100 kb 5' and 3' of the incident polymorphic variant.
[0043] In certain embodiments, polymorphic variants associated with type II diabetes are identified iteratively. For example, a first proximal polymorphic variant is associated with type II diabetes using the methods described above and then another polymorphic variant proximal to the first proximal polymorphic variant is identified (e.g., publicly disclosed or discovered) and the presence or absence of an association of one or more other polymorphic variants proximal to the first proximal polymorphic variant with type II diabetes is determined.
[0044] The methods described herein are useful for identifying or discovering additional polymorphic variants that may be used to further characterize a gene, region or loci associated with a condition, a disease (e.g., type II diabetes), or a disorder. For example, allelotyping or genotyping data from the additional polymorphic variants may be used to identify a functional mutation or a region of linkage disequilibrium. In certain embodiments, polymorphic variants identified or discovered within a region comprising the first polymorphic variant associated with type II diabetes are genotyped using the genetic methods and sample selection techniques described herein, and it can be determined whether those polymorphic variants are in linkage disequilibrium with the first polymorphic variant. The size of the region in linkage disequilibrium with the first polymorphic variant also can be assessed using these genotyping methods. Thus, provided herein are methods for determining whether a polymorphic variant is in linkage disequilibrium with a first polymorphic variant associated with type II diabetes, and such information can be used in prognosis/diagnosis methods described herein.
Isolated Nucleic Acids
[0045] Featured herein are isolated SLC22A1, TTN, LOCJJ 2609, TRPSJ, PRAME, ZNF22J, FOXA2, JAMS or GLB nucleic acid variants depicted in SEQ ID NO: 1-20, and substantially identical nucleic acids thereof. A nucleic acid variant may be represented on one or both strands in a double-stranded nucleic acid or on one chromosomal complement (heterozygous) or both chromosomal complements (homozygous)).
[0046] As used herein, the term "nucleic acid" includes DNA molecules (e.g., a complementary DNA (cDNA) and genomic DNA (gDNA)) and RNA molecules (e.g., mRNA, rRNA, siRNA and tRNA) and analogs of DNA or RNA, for example, by use of nucleotide analogs. The nucleic acid molecule can be single-stranded and it is often double-stranded. The term "isolated or purified nucleic acid" refers to nucleic acids that are separated from other nucleic acids present in the natural source of the nucleic acid. For example, with regard to genomic DNA, the term "isolated" includes nucleic acids which are separated from the chromosome with which the genomic DNA is naturally associated. An "isolated" nucleic acid is often free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and/or 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated nucleic acid molecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of 5' and/or 3' nucleotide sequences which flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived. Moreover, an "isolated" nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized. As used herein, the term "gene" refers to a nucleotide sequence that encodes a polypeptide.
[0047] The nucleic acid often comprises a part of or all of a nucleotide sequence in SEQ ID NO: 1-20, or a substantially identical sequence thereof. Such a nucleotide sequence sometimes is a 5' and/or 3' sequence flanking a polymorphic variant described above that is 5-1000 nucleotides in length, or in some embodiments 5-500, 5-100, 5-75, 5-50, 5-45, 5-40, 5-35, 5-30, 5-25 or 5-20 nucleotides in length. In certain embodiments, the nucleic acid comprises one or more of the following nucleotides: a guanine at position 522 of SEQ ID NO:5 or a thymine at position 19890 of SEQ ID NO: 5. Other embodiments are directed to methods of identifying a polymorphic variation at one or more positions in a nucleic acid (e.g., genotyping at one or more positions in the nucleic acid), such as at a position corresponding to SNP AA or SNP AB in the PRAME gene.
[0048] Also included herein are nucleic acid fragments. These fragments often are a nucleotide sequence identical to a nucleotide sequence of SEQ ID NO: 1-20, a nucleotide sequence substantially identical to a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence that is complementary to the foregoing. The nucleic acid fragment may be identical, substantially identical or homologous to a nucleotide sequence in an exon or an intron in a nucleotide sequence of SEQ ID NO: 1-9, and may encode a domain or part of a domain of a polypeptide. Sometimes, the fragment will comprises one or more of the polymorphic variations described herein as being associated with type II diabetes. The nucleic acid fragment is often 50, 100, or 200 or fewer base pairs in length, and is sometimes about 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 2000, 3000, 4000, 5000, 10000, 15000, or 20000 base pairs in length. A nucleic acid fragment that is complementary to a nucleotide sequence identical or substantially identical to a nucleotide sequence in SEQ ID-NO: 1-20 and hybridizes to such a nucleotide sequence under stringent conditions is often referred to as a "probe." Nucleic acid fragments often include one or more polymorphic sites, or sometimes have an end that is adjacent to a polymorphic site as described hereafter. JAM3 nucleic acid fragments sometimes encode an immunoglobulin domain cell adhesion molecule (cam) subfamily region from positions 283 to 534 and 577 to 858 of the mRNA sequence (positions 87 to 170 and 185 to 278, respectively, of the polypeptide sequence) or neural cell adhesion molecule Ll region from positions 340 to 843 of the mRNA sequence (positions 106 to 273 of the polypeptide sequence), for example. GLI3 nucleic acid fragments sometimes encode a C2H2-type Zn- finger protein region from positions 1111 to 1935 of the mRNA sequence (positions 353 to 627 of the polypeptide sequence) or a zinc finger region from positions 1492 to 1950 of the mRNA sequence (positions 480 to 632 of the polypeptide sequence), for example. [0049] An example of a nucleic acid fragment is an oligonucleotide. As used herein, the term "oligonucleotide" refers to a nucleic acid comprising about 8 to about 50 covalently linked nucleotides, often comprising from about 8 to about 35 nucleotides, and more often from about 10 to about 25 nucleotides. The backbone and nucleotides within an oligonucleotide may be the same as those of naturally occurring nucleic acids, or analogs or derivatives of naturally occurring nucleic acids, provided that oligonucleotides having such analogs or derivatives retain the ability to hybridize specifically to a nucleic acid comprising a targeted polymorphism. Oligonucleotides described herein may be used as hybridization probes or as components of prognostic or diagnostic assays, for example, as described herein.
[0050] Oligonucleotides are typically synthesized using standard methods and equipment, such as the ABFM3900 High Throughput DNA Synthesizer and the EXPEDITE™ 8909 Nucleic Acid Synthesizer, both of which are available from Applied Biosystems (Foster City, CA). Analogs and derivatives are exemplified in U.S. Pat. Nos. 4,469,863; 5,536,821; 5,541,306; 5,637,683; 5,637,684; 5,700,922; 5,717,083; 5,719,262; 5,739,308; 5,773,601; 5,886,165; 5,929,226; 5,977,296; 6,140,482; WO 00/56746; WO 01/14398, and related publications. Methods for synthesizing oligonucleotides comprising such analogs or derivatives are disclosed, for example, in the patent publications cited above and in U.S. Pat. Nos. 5,614,622; 5,739,314; 5,955,599; 5,962,674; 6,117,992; in WO 00/75372; and in related publications.
[0051] Oligonucleotides may also be linked to a second moiety. The second moiety may be an additional nucleotide sequence such as a tail sequence (e.g., a polyadenosine tail), an adapter sequence (e.g., phage Ml 3 universal tail sequence), and others. Alternatively, the second moiety may be a non-nucleotide moiety such as a moiety which facilitates linkage to a solid support or a label to facilitate detection of the oligonucleotide. Such labels include, without limitation, a radioactive label, a fluorescent label, a chemiluminescent label, a paramagnetic label, and the like. The second moiety may be attached to any position of the oligonucleotide, provided the oligonucleotide can hybridize to the nucleic acid comprising the polymorphism.
Uses of Nucleic Acid Sequence
[0052] Nucleic acid coding sequences (e.g., SEQ ID NO: 10-20) may be used for diagnostic purposes for detection and control of polypeptide expression. Also, included herein are oligonucleotide sequences such as antisense RNA, small-interfering RNA (siRNA) and DNA molecules and ribozymes that function to inhibit translation of a polypeptide. Antisense techniques and RNA interference techniques are known in the art and are described herein.
[0053] Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA. The mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage. For example, hammerhead motif ribozyme molecules may be engineered that specifically and efficiently catalyze endonucleolytic cleavage of RNA sequences corresponding to or complementary to SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequences. Specific ribozyme cleavage sites within any potential RNA target are initially identified by scanning the target molecule for ribozyme cleavage sites which include the following sequences, GUA, GUU and GUC. Once identified, short RNA sequences of between fifteen (15) and twenty (20) ribonucleotides corresponding to the region of the target gene containing the cleavage site may be evaluated for predicted structural features such as secondary structure that may render the oligonucleotide sequence unsuitable. The suitability of candidate targets may also be evaluated by testing their accessibility to hybridization with complementary oligonucleotides, using ribonuclease protection assays.
[0054] Antisense RNA and DNA molecules, siRNA and ribozymes may be prepared by any method known in the art for the synthesis of RNA molecules. These include techniques for chemically synthesizing oligodeoxyribonucleotides well known in the art such as solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding the antisense RNA molecule. Such DNA sequences may be incorporated into a wide variety of vectors which incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructs that synthesize antisense RNA constitutively or inducibly, depending on the promoter used, can be introduced stably into cell lines.
[0055] DNA encoding a polypeptide also may have a number of uses for the diagnosis of diseases, including type II diabetes, resulting from aberrant expression of a target gene described herein. For example, the nucleic acid sequence may be used in hybridization assays of biopsies or autopsies to diagnose abnormalities of expression or function (e.g., Southern or Northern blot analysis, in situ hybridization assays).
[0056] In addition, the expression of a polypeptide during embryonic development may also be determined using nucleic acid encoding the polypeptide. As addressed, infra, production of functionally impaired polypeptide is the cause of various disease states, such as type II diabetes. In situ hybridizations using polypeptide as a probe may be employed to predict problems related to type II diabetes. Further, as indicated, infra, administration of human active polypeptide, recombinantly produced as described herein, may be used to treat disease states related to functionally impaired polypeptide. Alternatively, gene therapy approaches may be employed to remedy deficiencies of functional polypeptide or to replace or compete with dysfunctional polypeptide. Expression Vectors. Host Cells, and Genetically Engineered Cells
[0057] Provided herein are nucleic acid vectors, often expression vectors, which contain a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLIS nucleotide sequence or a substantially identical sequence thereof. As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked and can include a plasmid, cosmid, or viral vector. The vector can be capable of autonomous replication or it can integrate into a host DNA. Viral vectors may include replication defective retroviruses, adenoviruses and adeno-associated viruses for example.
[0058] A vector can include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLI3 nucleotide sequence in a form suitable for expression of an encoded target polypeptide or target nucleic acid in a host cell. A "target polypeptide" is a polypeptide encoded by & SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence or a substantially identical nucleotide sequence thereof. The recombinant expression vector typically includes one or more regulatory sequences operatively linked to the nucleic acid sequence to be expressed. The term "regulatory sequence" includes promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence, as well as tissue-specific regulatory and/or inducible sequences. The design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, and the like. Expression vectors can be introduced into host cells to produce target polypeptides, including fusion polypeptides.
[0059] Recombinant expression vectors can be designed for expression of target polypeptides in prokaryotic or eukaryotic cells. For example, target polypeptides can be expressed in E. coli, insect cells (e.g., using baculovirus expression vectors), yeast cells, or mammalian cells. Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.
[0060] Expression of polypeptides in prokaryotes is most often carried out in E. coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion polypeptides. Fusion vectors add a number of amino acids to a polypeptide encoded therein, usually to the amino terminus of the recombinant polypeptide. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant polypeptide; 2) to increase the solubility of the recombinant polypeptide; and 3) to aid in the purification of the recombinant polypeptide by acting as a ligand in affinity purification. Often, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant polypeptide to enable separation of the recombinant polypeptide from the fusion moiety subsequent to purification of the fusion polypeptide. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith & Johnson, Gene 67: 31-40 (1988)), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding polypeptide, or polypeptide A, respectively, to the target recombinant polypeptide.
[0061] Purified fusion polypeptides can be used in screening assays and to generate antibodies specific for target polypeptides. In a therapeutic embodiment, fusion polypeptide expressed in a retroviral expression vector is used to infect bone marrow cells that are subsequently transplanted into irradiated recipients. The pathology of the subject recipient is then examined after sufficient time has passed {e.g., six (6) weeks).
[0062] Expressing the polypeptide in host bacteria with an impaired capacity to proteolytically cleave the recombinant polypeptide is often used to maximize recombinant polypeptide expression (Gottesman, S., Gene Expression Technology: Methods in Enzymology, Academic Press, San Diego, California 185: 119-128 (1990)). Another strategy is to alter the nucleotide sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in E. coli (Wada et al, Nucleic Acids Res. 20: 2111-2118 (1992)). Such alteration of nucleotide sequences can be carried out by standard DNA synthesis techniques.
[0063] When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. Recombinant mammalian expression vectors are often capable of directing expression of the nucleic acid in a particular cell type (e.g.', tissue-specific regulatory elements are used to express the nucleic acid). Non-limiting examples of suitable tissue-specific promoters include an albumin promoter (liver-specific; Pinkert et al, Genes Dev. 1: 268-277 (1987)), lymphoid-specific promoters (Calame & Eaton, Adv. Immunol. 43: 235-275 (1988)), promoters of T cell receptors (Winoto & Baltimore, EMBO J. 8: 729-733 (1989)) promoters of immunoglobulins (Banerji et al, Cell 33: 729-740 (1983); Queen & Baltimore, Cell 33: 741-948 (1983)), neuron-specific promoters (e.g., the neurofilament promoter; Byrne & Ruddle, Proc. Natl. Acad. Sci. USA 86: 5473-5477 (1989)), pancreas-specific promoters (Edlund et al, Science 230: 912-916 (1985)), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are sometimes utilized, for example, the murine hox promoters (Kessel & Grass, Science 249: 31A-119 (1990)) and the α-fetopolypeptide promoter (Campes & Tilghman, Genes Dev. 3: 537-546 (1989)).
[0064] A SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid may also be cloned.into an expression vector in an antisense orientation. Regulatory sequences (e.g., viral promoters and/or enhancers) operatively linked to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid cloned in the antisense orientation can be chosen for directing constitutive, tissue specific or cell type specific expression of antisense RNA in a variety of cell types. Antisense expression vectors can be in the form of a recombinant plasmid, phagemid or attenuated virus. For a discussion of the regulation of gene expression using antisense genes see, e.g., Weintraub et ah, Antisense RNA as a molecular tool for genetic analysis, Reviews - Trends in Genetics, Vol. 1(1) (1986).
[0065] Also provided herein are host cells that include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLI3 nucleotide sequence within a recombinant expression vector or a fragment of such a nucleotide sequence which facilitate homologous recombination into a specific site of the host cell genome. The terms "host cell" and "recombinant host cell" are used interchangeably herein. Such terms refer not only to the particular subject cell but rather also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein. A host cell can be any prokaryotic or eukaryotic cell. For example, a target polypeptide can be expressed in bacterial cells such as E. coli, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art.
[0066] Vectors can be introduced into host cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, transduction/infection, DEAE-dextran-mediated transfection, lipofection, or electroporation.
[0067] A host cell provided herein can be used to produce (i.e., express) a target polypeptide or a substantially identical polypeptide thereof. Accordingly, further provided are methods for producing a target polypeptide using host cells described herein. In one embodiment, the method includes culturing host cells into which a recombinant expression vector encoding a target polypeptide has been introduced in a suitable medium such that a target polypeptide is produced. In another embodiment, the method further includes isolating a target polypeptide from the medium or the host cell. [0068] Also provided are cells or purified preparations of cells which include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB transgene, or which otherwise misexpress target polypeptide. Cell preparations can consist of human or non-human cells, e.g., rodent cells, e.g., mouse or rat cells, rabbit cells, or pig cells. In preferred embodiments, the cell or cells include a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB transgene (e.g., a heterologous form of a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLL3 gene, such as a human gene expressed in non-human cells). The transgene can be misexpressed, e.g., overexpressed or underexpressed. In other preferred embodiments, the cell or cells include a gene which misexpress an endogenous target polypeptide (e.g., expression of a gene is disrupted, also known as a knockout). Such cells can serve as a model for studying disorders which are related to mutated or mis-expressed alleles or for use in drug screening. Also provided are human cells (e.g., a hematopoietic stem cells) transformed with a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid.
[0069] Also provided are cells or a purified preparation thereof (e.g. , human cells) in which an endogenous SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid is under the control of a regulatory sequence that does not normally control the expression of the endogenous gene corresponding to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequence. The expression characteristics of an endogenous gene within a cell (e.g., a cell line or microorganism) can be modified by inserting a heterologous DNA regulatory element into the genome of the cell such that the inserted regulatory element is operably linked to the corresponding endogenous gene. For example, an endogenous corresponding gene (e.g., a gene which is "transcriptionally silent," not normally expressed, or expressed only at very low levels) may be activated by inserting a regulatory element which is capable of promoting the expression of a normally expressed gene product in that cell. Techniques such as targeted homologous recombinations, can be used to insert the heterologous DNA as described in, e.g., Chappel, US 5,272,071; WO 91/06667, published on May 16, 1991.
Transgenic Animals
[0070] Non-human transgenic animals that express a heterologous target polypeptide (e.g., expressed from a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid or substantially identical sequence thereof) can be generated. Such animals are useful for studying the function and/or activity of a target polypeptide and for identifying and/or evaluating modulators of the activity of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acids and encoded polypeptides. As used herein, a "transgenic animal" is a non-human animal such as a mammal (e.g., a non-human primate such as chimpanzee, baboon, or macaque; an ungulate such as an equine, bovine, or caprine; or a rodent such as a rat, a mouse, or an Israeli sand rat), a bird {e.g., a chicken or a turkey), an amphibian (e.g., a frog, salamander, or newt), or an insect (e.g., Drosophilα melαnogαstef), in which one or more of the cells of the animal includes a transgene. A transgene is exogenous DNA or a rearrangement (e.g., a deletion of endogenous chromosomal DNA) that is often integrated into or occurs in the genome of cells in a transgenic animal. A transgene can direct expression of an encoded gene product in one or more cell types or tissues of the transgenic animal, and other transgenes can reduce expression (e.g., a knockout). Thus, a transgenic animal can be one in which an endogenous nucleic acid homologous to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal (e.g., an embryonic cell of the animal) prior to development of the animal.
[0071] Intronic sequences and polyadenylation signals can also be included in the transgene to increase expression efficiency of the transgene. One or more tissue-specific regulatory sequences can be operably linked to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence to direct expression of an encoded polypeptide to particular cells. A transgenic founder animal can be identified based upon the presence of a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence in its genome and/or expression of encoded mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a SLC22A1, TTN, LOCI 12609, TRPSl, FRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence can further be bred to other transgenic animals carrying other transgenes.
[0072] Target polypeptides can be expressed in transgenic animals or plants by introducing, for example, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleic acid into the genome of an animal that encodes the target polypeptide. In preferred embodiments the nucleic acid is placed under the control of a tissue specific promoter, e.g., a milk or egg specific promoter, and recovered from the milk or eggs produced by the animal. Also included is a population of cells from a transgenic animal.
Target Polypeptides
[0073] Also featured herein are isolated target polypeptides, which are encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence (e.g., SEQ ID NO: 1-20) or a substantially identical nucleotide sequence thereof, such as the polypeptides having amino acid sequences in SEQ ID NO: 21-30. The term "polypeptide" as used herein includes proteins and peptides. An "isolated" or "purified" polypeptide or protein is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. In one embodiment, the language "substantially free" means preparation of a target polypeptide having less than about 30%, 20%, 10% and more preferably 5% (by dry weight), of non-target polypeptide (also referred to herein as a "contaminating protein"), or of chemical precursors or non-target chemicals. When the target polypeptide or a biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, specifically, where culture medium represents less than about 20%, sometimes less than about 10%, and often less than about 5% of the volume of the polypeptide preparation. Isolated or purified1 target polypeptide preparations are sometimes 0.01 milligrams or more or 0.1 milligrams or more, and often 1.0 milligrams or more and 10 milligrams or more in dry weight.
[0074] Further included herein are target polypeptide fragments. The polypeptide fragment may be a domain or part of a domain of a target polypeptide. For example, a fragment sometimes is a FOXA2 polypeptide domain encoded by a nucleotide sequence referenced in SEQ ID NO: 17 or 18. The polypeptide fragment may have increased, decreased or unexpected biological activity. The polypeptide fragment is often 50 or fewer, 100 or fewer, or 200 or fewer amino acids in length, and is sometimes 300, 400, 500, 600, 700, or 900 or fewer amino acids in length.
[0075] Substantially identical target polypeptides may depart from the amino acid sequences of target polypeptides in different manners. For example, conservative amino acid modifications may be introduced at one or more positions in the amino acid sequences of target polypeptides. A "conservative amino acid substitution" is one in which the amino acid is replaced by another amino acid having a similar structure and/or chemical function. Families of amino acid residues having similar structures and functions are well known. These families include amino acids with basic side chains {e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Also, essential and non-essential amino acids may be replaced. A "non-essential" amino acid is one that can be altered without abolishing or substantially altering the biological function of a target polypeptide, whereas altering an "essential" amino acid abolishes or substantially alters the biological function of a target polypeptide. Amino acids that are conserved among target polypeptides are typically essential amino acids.
[0076] Also, target polypeptides may exist as chimeric or fusion polypeptides. As used herein, a target "chimeric polypeptide" or target "fusion polypeptide" includes a target polypeptide linked to a non-target polypeptide. A "non-target polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a polypeptide which is not substantially identical to the target polypeptide, which includes, for example, a polypeptide that is different from the target polypeptide and derived from the same or a different organism. The target polypeptide in the fusion polypeptide can correspond to an entire or nearly entire target polypeptide or a fragment thereof. The non-target polypeptide can be fused to the N-terminus or C-terminus of the target polypeptide.
[0077] Fusion polypeptides can include a moiety having high affinity for a ligand. For example, the fusion polypeptide can be a GST-target fusion polypeptide in which the target sequences are fused to the C-terminus of the GST sequences, or a polyhistidine-target fusion polypeptide in which the target polypeptide is fused at the N- or C-terminus to a string of histidine residues. Such fusion polypeptides can facilitate purification of recombinant target polypeptide. Expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide), and a nucleotide sequence in SEQ ID NO: 1-20, or a substantially identical nucleotide sequence thereof, can be cloned into an expression vector such that the fusion moiety is linked in- frame to the target polypeptide. Further, the fusion polypeptide can be a target polypeptide containing a heterologous signal sequence at its N-terminus. In certain host cells (e.g., mammalian host cells), expression, secretion, cellular internalization, and cellular localization of a target polypeptide can be increased through use of a heterologous signal sequence. Fusion polypeptides can also include all or a part of a serum polypeptide (e.g., an IgG constant region or human serum albumin).
[0078] Target polypeptides can be incorporated into pharmaceutical compositions and administered to a subject in vivo. Administration of these target polypeptides can be used to affect the bioavailability of a substrate of the target polypeptide and may effectively increase target polypeptide biological activity in a cell. Target fusion polypeptides may be useful therapeutically for the treatment of disorders caused by, for example, (i) aberrant modification or mutation of a gene encoding a target polypeptide; (ii) mis-regulation of the gene encoding the target polypeptide; and (iii) aberrant post-translational modification of a target polypeptide. Also, target polypeptides can be used as immunogens to produce anti-target antibodies in a subject, to purify target polypeptide ligands or binding partners, and in screening assays to identify molecules which inhibit or enhance the interaction of a target polypeptide with a substrate.
[0079] hi addition, polypeptides can be chemically synthesized using techniques known in the art (See, e.g., Creighton, 1983 Proteins. New York, N.Y.: W. H. Freeman and Company; and Hunkapiller et al, (1984) Nature July 12 -18;310(5973):105-ll). For example, a relative short fragment can be synthesized by use of a peptide synthesizer. Furthermore, if desired, non-classical amino acids or chemical amino acid analogs can be introduced as a substitution or addition into the fragment sequence. Non-classical amino acids include, but are not limited to, to the D-isomers of the common amino acids, 2,4-diaminobutyric acid, a-amino isobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, g-Abu, e-Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3- amino propionic acid, ornithine, norleucine, norvaline, hydroxyproline, sarcosine, citrulline, homocitrulline, cysteic acid, t-butylglycine, t-butylalanine, phenylglycine, cyclohexylalanine, b- alanine, fluoroamino acids, designer amino acids such as b-methyl amino acids, Ca-methyl amino acids, Na-methyl amino acids, and amino acid analogs in general. Furthermore, the amino acid can be D (dextrorotary) or L (levorotary).
[0080] Polypeptides and polypeptide fragments sometimes are differentially modified during or after translation, e.g., by glycosylation, acetylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to an antibody molecule or other cellular ligand, etc. Any of numerous chemical modifications may be carried out by known techniques, including but not limited, to specific chemical cleavage by cyanogen bromide, trypsin, chymotrypsin, papain, V8 protease, NaBH4; acetylation, formylation, oxidation, reduction; metabolic synthesis in the presence of tunicamycin; and the like. Additional post-translational modifications include, for example, N-linked or O-linked carbohydrate chains, processing of N- terminal or C-terminal ends), attachment of chemical moieties to the amino acid backbone, chemical modifications of N-linked or O-linked carbohydrate chains, and addition or deletion of an N-terminal methionine residue as a result of prokaryotic host cell expression. The polypeptide fragments may also be modified with a detectable label, such as an enzymatic, fluorescent, isotopic or affinity label to allow for detection and isolation of the polypeptide.
[0081] Also provided are chemically modified derivatives of polypeptides that can provide additional advantages such as increased solubility, stability and circulating time of the polypeptide, or decreased immunogenicity (see e.g., U.S. Pat. No: 4,179,337. The chemical moieties for derivitization may be selected from water soluble polymers such as polyethylene glycol, ethylene glycol/propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol and the like. The polypeptides may be modified at random positions within the molecule, or at predetermined positions within the molecule and may include one, two, three or more attached chemical moieties.
[0082] The polymer may be of any molecular weight, and may be branched or unbranched. For polyethylene glycol, the preferred molecular weight is between about 1 kDa and about 100 kDa (the term "about" indicating that in preparations of polyethylene glycol, some molecules will weigh more, some less, than the stated molecular weight) for ease in handling and manufacturing. Other sizes may be used, depending on the desired therapeutic profile (e.g., the duration of sustained release desired, the effects, if any on biological activity, the ease in handling, the degree or lack of antigenicity and other known effects of the polyethylene glycol to a therapeutic protein or analog).
[0083] The polymers should be attached to the polypeptide with consideration of effects on functional or antigenic domains of the polypeptide. There are a number of attachment methods available to those skilled in the art (e.g., EP 0 401 384 (coupling PEG to G-CSF) and Malik et al. (1992) Exp Hematol. September;20(8): 1028-35 (pegylation of GM-CSF using tresyl chloride)). For example, polyethylene glycol may be covalently bound through amino acid residues via a reactive group, such as a free amino or carboxyl group. Reactive groups are those to which an activated polyethylene glycol molecule may be bound. The amino acid residues having a free amino group may include lysine residues and the N-terminal amino acid residues; those having a free carboxyl group may include aspartic acid residues, glutamic acid residues and the C-terminal amino acid residue. Sulfhydryl groups may also be used as a reactive group for attaching the polyethylene glycol molecules. For therapeutic purposes, the attachment sometimes is at an amino group, such as attachment at the N-terminus or lysine group.
[0084] Proteins can be chemically modified at the N-terminus. Using polyethylene glycol as an illustration of such a composition, one may select from a variety of polyethylene glycol molecules (by molecular weight, branching, and the like), the proportion of polyethylene glycol molecules to protein (polypeptide) molecules in the reaction mix, the type of pegylation reaction to be performed, and the method of obtaining the selected N-terminally pegylated protein. The method of obtaining the N-terminally pegylated preparation (i.e., separating this moiety from other monopegylated moieties if necessary) may be by purification of the N-terminally pegylated material from a population of pegylated protein molecules. Selective proteins chemically modified at the N- terminus may be accomplished by reductive alkylation, which exploits differential reactivity of different types of primary amino groups (lysine versus the N-terminal) available for derivatization in a particular protein. Under the appropriate reaction conditions, substantially selective derivatization of the protein at the N-terminus with a carbonyl group containing polymer is achieved.
Substantially Identical Nucleic Acids and Polypeptides
[0085] Nucleotide sequences and polypeptide sequences that are substantially identical to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence and the target polypeptide sequences encoded by those nucleotide sequences, respectively, are included herein. The term "substantially identical" as used herein refers to two or more nucleic acids or polypeptides sharing one or more identical nucleotide sequences or polypeptide sequences, respectively. Included are nucleotide sequences or polypeptide sequences that are 55% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 95% or more (each often within a 1%, 2%, 3% or 4% variability) identical to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence or the encoded target polypeptide amino acid sequences. One test for determining whether two nucleic acids are substantially identical is to determine the percent of identical nucleotide sequences or polypeptide sequences shared between the nucleic acids or polypeptides.
[0086] Calculations of sequence identity are often performed as follows. Sequences are aligned for optimal comparison purposes (e.g. , gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The length of a reference sequence aligned for comparison purposes is sometimes 30% or more, 40% or more, 50% or more, often 60% or more, and more often 70% or more, 80% or more, 90% or more, or 100% of the length of the reference sequence. The nucleotides or amino acids at corresponding nucleotide or polypeptide positions, respectively, are then compared among the two sequences. When a position in the first sequence is occupied by the same nucleotide or amino acid as the corresponding position in the second sequence, the nucleotides or amino acids are deemed to be identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, introduced for optimal alignment of the two sequences.
[0087] Comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. Percent identity between two amino acid or nucleotide sequences can be determined using the algorithm of Meyers & Miller, CABIOS 4: 11-17 (1989), which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. Also, percent identity between two amino acid sequences can be determined using the Needleman & Wunsch, J. MoI. Biol. 48: 444-453 (1970) algorithm which has been incorporated into the GAP program in the GCG software package (available at the http address www.gcg.com), using either a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. Percent identity between two nucleotide sequences can be determined using the GAP program in the GCG software package (available at http address www.gcg.com), using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or 6. A set of parameters often used is a Blossum 62 scoring matrix with a gap open penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5.
[0088] Another manner for determining if two nucleic acids are substantially identical is to assess whether a polynucleotide homologous to one nucleic acid will hybridize to the other nucleic acid under stringent conditions. As use herein, the term "stringent conditions" refers to conditions for hybridization and washing. Stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. , 6.3.1-6.3.6 (1989). Aqueous and non-aqueous methods are described in that reference and either can be used. An example of stringent hybridization conditions is hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 500C. Another example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 55°C. A further example of stringent hybridization conditions is hybridization in 6X sodium chloride/sodium citrate (SSC) at about 450C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 600C. Often, stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 65°C. More often, stringency conditions are 0.5M sodium phosphate, 7% SDS at 65°C, followed by one or more washes at 0.2X SSC, 1% SDS at 65°C.
[0089] An example of a substantially identical nucleotide sequence to a nucleotide sequence in SEQ ID NO: 1-20 is one that has a different nucleotide sequence but still encodes the same polypeptide sequence encoded by the nucleotide sequence in SEQ ED NO: 1-20. Another example is a nucleotide sequence that encodes a polypeptide having a polypeptide sequence that is more than 70% or more identical to, sometimes more than 75% or more, 80% or more, or 85% or more identical to, and often more than 90% or more and 95% or more identical to a polypeptide sequence encoded by a nucleotide sequence in SEQ ID NO: 1-20. As used herein, "SEQ ID NO: 1-20" typically refers to one or more sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20. Many of the embodiments described herein are applicable to (a) a nucleotide sequence of SEQ DO NO: 1-20; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; (d) a fragment of a nucleotide sequence of (a), (b), or (c); and/or a nucleotide sequence complementary to the nucleotide sequences of (a), (b), (c) and/or (d), where nucleotide sequences of (b) and (c), fragments of (b) and (c) and nucleotide sequences complementary to (b) and (c) are examples of substantially identical nucleotide sequences. Examples of substantially identical nucleotide sequences include nucleotide sequences from subjects that differ by naturally occurring genetic variance, which sometimes is referred to as background genetic variance {e.g., nucleotide sequences differing by natural genetic variance sometimes are 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to one another).
[0090] Nucleotide sequences in SEQ ID NO: 1-20 and amino acid sequences of encoded polypeptides can be used as "query sequences" to perform a search against public databases to identify other family members or related sequences, for example. Such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul et al, J. MoI. Biol 215: 403- 10 (1990). BLAST nucleotide searches can be performed with the NBLAST program, score = 100, wordlength = 12 to obtain nucleotide sequences homologous to nucleotide sequences in SEQ ID NO: 1-20. BLAST polypeptide searches can be performed with the XBLAST program, score = 50, wordlength = 3 to obtain amino acid sequences homologous to polypeptides encoded by the nucleotide sequences of SEQ ID NO: 1-20. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al, Nucleic Acids Res. 25(17): 3389- 3402 (1997). When utilizing BLAST and Gapped BLAST programs, default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used {see the http address www.ncbi.nlm.nih.gov).
[0091] A nucleic acid that is substantially identical to a nucleotide sequence in SEQ ID NO: 1- 20 may include polymorphic sites at positions equivalent to those described herein when the sequences are aligned. For example, using the alignment procedures described herein, SNPs in a sequence substantially identical to a sequence in SEQ ID NO: 1-20 can be identified at nucleotide positions that match with or correspond to (i.e., align) nucleotides at SNP positions in each nucleotide sequence in SEQ ID NO: 1-20. Also, where a polymorphic variation results in an insertion or deletion, insertion or deletion of a nucleotide sequence from a reference sequence can change the relative positions of other polymorphic sites in the nucleotide sequence.
[0092] Substantially identical nucleotide and polypeptide sequences include those that are naturally occurring, such as allelic variants (same locus), splice variants, homologs (different locus), and orthologs (different organism) or can be non-naturally occurring. Non-naturally occurring variants can be generated by mutagenesis techniques, including those applied to polynucleotides, cells, or organisms. The variants can contain nucleotide substitutions, deletions, inversions and insertions. Variation can occur in either or both the coding and non-coding regions. The variations can produce both conservative and non-conservative amino acid substitutions (as compared in the encoded product). Orthologs, homologs, allelic variants, and splice variants can be identified using methods known in the art. These variants normally comprise a nucleotide sequence encoding a polypeptide that is 50% or more, about 55% or more, often about 70-75% or more or about 80-85% or more, and sometimes about 90-95% or more identical to the amino acid sequences of target polypeptides or a fragment thereof. Such nucleic acid molecules can readily be identified as being able to hybridize under stringent conditions to a nucleotide sequence in SEQ ID NO: 1-20 or a fragment of this sequence. Nucleic acid molecules corresponding to orthologs, homologs, and allelic variants of a nucleotide sequence in SEQ ID NO: 1-20 can further be identified by mapping the sequence to the same chromosome or locus as the nucleotide sequence in SEQ ID NO: 1-20. [0093] Also, substantially identical nucleotide sequences may include codons that are altered with respect to the naturally occurring sequence for enhancing expression of a target polypeptide in a particular expression system. For example, the nucleic acid can be one in which one OF more codons are altered, and often 10% or more or 20% or more of the codons are altered for optimized expression in bacteria (e.g., E. coli.), yeast (e.g., S. cervesiae), human (e.g., 293 cells), insect or rodent (e.g., hamster)- cells.
Methods for Identifying Subjects at Risk of Diabetes and Risk of Diabetes in a Subject [0094] Methods for prognosing and diagnosing type II diabetes and its related disorders (e.g., metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia) are included herein. These methods include detecting the presence or absence of one or more polymorphic variations in a nucleotide sequence associated with type II diabetes, such as variants in or around the loci set forth herein, or a substantially identical sequence thereof, in a sample from a subject, where the presence of a polymorphic variant described herein is indicative of a risk of type II diabetes or one or more type II diabetes related disorders (e.g., metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia). Determining a risk of type II diabetes refers to determining whether an individual is at an increased risk of type II diabetes (e.g., intermediate risk or higher risk).
[0095] Thus, featured herein is a method for identifying a subject who is at risk of type II diabetes, which comprises detecting type II diabetes-associated aberration in a nucleic acid sample from the subject. An embodiment is a method for detecting a risk of type II diabetes in a subject, which comprises detecting the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject, where the nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO: 1-20; (b) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; and (d) a fragment of a nucleotide sequence of (a), (b), or (c) comprising the polymorphic site; whereby the presence of the polymorphic variation is indicative of a predisposition to type II diabetes in the subject. In certain embodiments, polymorphic variants at the positions described herein are detected for determining a risk of type II diabetes, and polymorphic variants at positions in linkage disequilibrium with these positions are detected for determining a risk of type II diabetes. As used herein, "SEQ ID NO: 1-20" refers to individual sequences in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and/or 20.
[0096] Results from prognostic tests may be combined with other test results to diagnose type II diabetes related disorders, including metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia. For example, prognostic results may be gathered, a patient sample may be ordered based on a determined predisposition to type II diabetes, the patient sample is analyzed, and the results of the analysis may be utilized to diagnose the type II diabetes related condition (e.g., metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia). Also type II diabetes diagnostic methods can be developed from studies used to generate prognostic methods in which populations are stratified into subpopulations having different progressions of a type II diabetes related disorder or condition. In another embodiment, prognostic results may be gathered, a patient's risk factors for developing type II diabetes (e.g., age, weight, race, dief} analyzed, and a patient sample may be ordered based on a determined predisposition to type II diabetes.
[0097] Risk of type II diabetes sometimes is expressed as a probability, such as an odds ratio, percentage, or risk factor. The risk sometimes is expressed as a relative risk with respect to a population average risk of type II diabetes, and sometimes is expressed as a relative risk with respect to the lowest risk group. Such relative risk assessments often are based upon penetrance values determined by statistical methods and are particularly useful to clinicians and insurance companies for assessing risk of type II diabetes (e.g., a clinician can target appropriate detection, prevention and therapeutic regimens to a patient after determining the patient's risk of type II diabetes, and an insurance company can fine tune actuarial tables based upon population genotype assessments of type II diabetes risk). Risk of type II diabetes sometimes is expressed as an odds ratio, which is the odds of a particular person having a genotype has or will develop type II diabetes with respect to another genotype group (e.g., the most disease protective genotype or population average). The risk often is based upon the presence or absence of one or more polymorphic variants described herein, and also may be based in part upon phenotypic traits of the individual being tested. In an embodiment, two or more polymorphic variations are detected in a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB locus. In certain embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variants are detected in the sample. Methods for calculating risk based upon patient data are well known (see, e.g., Agresti, Categorical Data Analysis, 2nd Ed. 2002. Wiley). Allelotyping and genotyping analyses may be carried out in populations other than those exemplified herein to enhance the predictive power of the prognostic method. [0098] The nucleic acid sample typically is isolated from a biological sample obtained from a subject. For example, nucleic acid can be isolated from blood, saliva, sputum, urine, cell scrapings, and biopsy tissue. The nucleic acid sample can be isolated from a biological sample using standard techniques, such as the technique described in Example 2. As used herein, the term "subject" refers primarily to humans but also refers to other mammals such as dogs, cats, and ungulates (e.g., cattle, sheep, and swine). Subjects also include avians (e.g., chickens and turkeys}, reptiles, and fish (e.g., salmon), as embodiments described herein can be adapted to nucleic acid samples isolated from any of these organisms. The nucleic acid sample may be isolated from the subject and then directly utilized in a method for determining the presence of a polymorphic variant, or alternatively, the sample may be isolated and then stored (e.g., frozen) for a period of time before being subjected to analysis.
[0099] The presence or absence of a polymorphic variant is determined using one or both chromosomal complements represented in the nucleic acid sample. Determining the presence or absence of a polymorphic variant in both chromosomal complements represented in a nucleic acid sample from a subject having a copy of each chromosome is useful for determining the zygosity of an individual for the polymorphic variant (i.e., whether the individual is homozygous or heterozygous for the polymorphic variant). Any oligonucleotide-based diagnostic may be utilized to determine whether a sample includes the presence or absence of a polymorphic variant in a sample. For example, primer extension methods, ligase sequence determination methods (e.g., U.S. Pat. Nos. 5,679,524 and 5,952,174, and WO 01/27326), mismatch sequence deteπnination methods (e.g., U.S. Pat. Nos. 5,851,770; 5,958,692; 6,110,684; and 6,183,958), microarray sequence determination methods, restriction fragment length polymorphism (RFLP), single strand conformation polymorphism detection (SSCP) (e.g., U.S. Pat. Nos. 5,891,625. and 6,013,499), PCR- based assays (e.g., TAQMAN® PCR System (Applied Biosystems)), and nucleotide sequencing methods may be used.
[0100] Oligonucleotide extension methods typically involve providing a pair of oligonucleotide primers in a polymerase chain reaction (PCR) or in other nucleic acid amplification methods for the purpose of amplifying a region from the nucleic acid sample that comprises the polymorphic variation. One oligonucleotide primer is complementary to a region 3' of the polymorphism and the other is complementary to a region 5' of the polymorphism. A PCR primer pair may be used in methods disclosed in U.S. Pat. Nos. 4,683,195; 4,683,202, 4,965,188; 5,656,493; 5,998,143; 6,140,054; WO 01/27327; and WO 01/27329 for example. PCR primer pairs may also be used in any commercially available machines that perform PCR, such as any of the GENEAMP® Systems available from Applied Biosystems. Also, those of ordinary skill in the art will be able to design oligonucleotide primers based upon a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence using knowledge available in the art.
[0101] Also provided is an extension oligonucleotide that hybridizes to the amplified fragment adjacent to the polymorphic variation. As used herein, the term "adjacent" refers to the 3' end of the extension oligonucleotide being often 1 nucleotide from the 5' end of the polymorphic site, and sometimes 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides from the 5' end of the polymorphic site, in the nucleic acid when the extension oligonucleotide is hybridized to the nucleic acid. The extension oligonucleotide then is extended by one or more nucleotides, and the number and/or type of nucleotides that are added to the extension oligonucleotide determine whether the polymorphic variant is present. Oligonucleotide extension methods are disclosed, for example, in U.S. Pat. Nos. 4,656,127; 4,851,331; 5,679,524; 5,834,189; 5,876,934; 5,908,755; 5,912,118; 5,976,802; 5,981,186; 6,004,744; 6,013,431; 6,017,702; 6,046,005; 6,087,095; 6,210,891; and WO 01/20039. Oligonucleotide extension methods using mass spectrometry are described, for example, in U.S. Pat. Nos. 5,547,835; 5,605,798; 5,691,141; 5,849,542; 5,869,242; 5,928,906; 6,043,031; and 6,194,144, and a method often utilized is described herein in Example 2.
[0102] A microarray can be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample. A microarray may include any oligonucleotides described herein, and methods for making and using oligonucleotide microarrays suitable for diagnostic use are disclosed in U.S. Pat. Nos. 5,492,806; 5,525,464; 5,589,330; 5,695,940; 5,849,483; 6,018,041; 6,045,996; 6,136,541; 6,142,681; 6,156,501; 6,197,506; 6,223,127; 6,225,625; 6,229,911; 6,239,273; WO 00/52625; WO 01/25485; and WO 01/29259. The microarray typically comprises a solid support and the oligonucleotides may be linked to this solid support by covalent bonds or by non-covalent interactions. The oligonucleotides may also be linked to the solid support directly or by a spacer molecule. A microarray may comprise one or more oligonucleotides complementary to a polymorphic site set forth herein.
[0103] A kit also may be utilized for determining whether a polymorphic variant is present or absent in a nucleic acid sample. A kit often comprises one or more pairs of oligonucleotide primers useful for amplifying a fragment of a nucleotide sequence of SEQ ID NO: 1-20 or a substantially identical sequence thereof, where the fragment includes a polymorphic site. The kit sometimes comprises a polymerizing agent, for example, a thermostable nucleic acid polymerase such as one disclosed in U.S. Pat. Nos. 4,889,818 or 6,077,664. Also, the kit often comprises an elongation oligonucleotide that hybridizes to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence in a nucleic acid sample adjacent to the polymorphic site. Where the kit includes an elongation oligonucleotide, it also often comprises chain elongating nucleotides, such as dATP, dTTP, dGTP, dCTP, and dITP, including analogs of dATP, dTTP, dGTP, dCTP and dITP, provided that such analogs are substrates for a thermostable nucleic acid polymerase and can be incorporated into a nucleic acid chain elongated from the extension oligonucleotide. Along with chain elongating nucleotides would be one or more chain terminating nucleotides such as ddATP, ddTTP, ddGTP, ddCTP, and the like. In an embodiment, the kit comprises one or more oligonucleotide primer pairs, a polymerizing agent, chain elongating nucleotides, at least one elongation oligonucleotide, and one or more chain terminating nucleotides. Kits optionally include buffers, vials, microtiter plates, and instructions for use.
[0104] An individual identified as being at risk of type II diabetes may be heterozygous or homozygous with respect to the allele associated with a higher risk of type II diabetes. A subject homozygous for an allele associated with an increased risk of type II diabetes is at a comparatively high risk of type II diabetes, a subject heterozygous for an allele associated with an increased risk of type II diabetes is at a comparatively intermediate risk of type II diabetes, and a subject homozygous for an allele associated with a decreased risk of type II diabetes, is at a comparatively low risk of type II diabetes. A genotype may be assessed for a complementary strand, such that the complementary nucleotide at a particular position is detected.
[0105] Also featured are methods for determining risk of type II diabetes, and/or identifying a subject at risk of type II diabetes by contacting a polypeptide or protein encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence from a subject with an antibody that specifically binds to an epitope associated with increased risk of type II diabetes in the polypeptide. In an embodiment, the antibody specifically binds to an epitope comprising a glycine at position 557 in a ZNF221 polypeptide.
Applications of Prognostic and Diagnostic Results to Pharmacogenomic Methods [0106] Pharmacogenomics is a discipline that involves, tailoring a treatment for a subject according to the subject's genotype as a particular treatment regimen may exert a differential effect depending upon the subject's genotype. For example, based upon the outcome of a prognostic test described herein, a clinician or physician may target pertinent information and preventative or therapeutic treatments to a subject who would be benefited by the information or treatment and avoid directing such information and treatments to a subject who would not be benefited (e.g., the treatment has no therapeutic effect and/or the subject experiences adverse side effects).
[0107] The following is an example of a pharmacogenomic embodiment. A particular treatment regimen can exert a differential effect depending upon the subject's genotype. Where a candidate therapeutic exhibits a significant interaction with a major allele and a comparatively weak interaction with a minor allele (e.g., an order of magnitude or greater difference in the interaction), such a therapeutic typically would not be administered to a subject genotyped as being homozygous for the minor allele, and sometimes not administered to a subject genotyped as being heterozygous for the minor allele. In another example, where a candidate therapeutic is not significantly toxic when administered to subjects who are homozygous for a major allele but ia comparatively toxic when administered to subjects heterozygous or homozygous for a minor allele, the candidate therapeutic is not typically administered to subjects who are genotyped as being heterozygous or homozygous with respect to the minor allele.
[0108] The methods described herein are applicable to pharmacogenomic methods for preventing, alleviating or treating type II diabetes conditions such as metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia. For example, a nucleic acid sample from an individual may be subjected to a prognostic test described herein. Where one or more polymorphic variations associated with increased risk of type II diabetes are identified in a subject, information for preventing or treating type II diabetes and/or one or more type II diabetes treatment regimens then may be prescribed to that subject.
[0109} In certain embodiments, a treatment or preventative regimen is specifically prescribed and/or administered to individuals, who will most benefit from it based upon their risk of developing type II diabetes assessed by the methods described herein. Thus, provided are methods for identifying a subject predisposed to type II diabetes and then prescribing a therapeutic or preventative regimen to individuals identified as having a predisposition. Thus, certain embodiments are directed to a method for reducing type II diabetes in a subject, which comprises: detecting the presence or absence of a polymorphic variant associated with type II diabetes in a nucleotide sequence in a nucleic acid sample from a subject, where the nucleotide sequence comprises a polynucleotide sequence selected from the group consisting of: (a) a nucleotide sequence of SEQ ID NO: 1-20; (b)- a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (c) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; and (d) a fragment of a polynucleotide sequence of (a), (b), or (c); and prescribing or administering a treatment regimen to a subject from whom the sample originated where the presence of a polymorphic variation associated with type II diabetes is detected in the nucleotide sequence. In these methods, predisposition results may be utilized in combination with other test results to diagnose type II diabetes associated conditions, such as metabolic disorders, syndrome X, obesity, insulin resistance, hyperglycemia.
[0110] Certain preventative treatments often are prescribed to subjects having a predisposition to type II diabetes and where the subject is diagnosed with type II diabetes or is diagnosed as having symptoms indicative of early stage type II diabetes, {e.g., impaired glucose tolerance, or IGT). For example, recent studies have highlighted the potential for intervention in IGT subjects to reduce progression to type II diabetes. One such study showed that over three years lifestyle intervention (targeting diet and exercise) reduced the risk of progressing from IGT to diabetes by 58% (The Diabetes Prevention Program. (1999) Diabetes Care 22:623-634). In a similar Finnish study, the cumulative incidence of diabetes after four years was 11% in the intervention group and 23% in the control group. During the trial, the risk of diabetes was. reduced by 58% in the intervention group (Tuomilehto et al. (2001) N. Eng. J Med. 344:1343-1350). Clearly there is great benefit in the early diagnosis and subsequent preventative treatment of type II diabetes.
[0111] The treatment sometimes is preventative (e.g., is prescribed or administered to reduce the probability that a type II diabetes associated condition arises or progresses);, sometimes is therapeutic, and sometimes delays, alleviates or halts the progression of a type II diabetes associated condition. Any known preventative or therapeutic treatment for alleviating or preventing the occurrence of a type II diabetes associated disorder is prescribed and/or administered. For example, the treatment sometimes includes changes in diet, increased exercise, and the administration of therapeutics such as sulphonylureas (and related insulin secretagogues), which increase insulin release from pancreatic islets; metformin (Glucophage™), which acts to reduce hepatic glucose production; peroxisome proliferator-activated receptor-gamma (PPAR) agonists (thiozolidinediones such as Avandia® and Actos®), which enhance insulin action; alpha- glucosidase inhibitors (e.g., Precose®, Voglibose®, and Miglitol®), which interfere with gut glucose absorption; and insulin itself, which suppresses glucose production and augments glucose utilization (Moller Nature 414, 821-827 (2001)).
[0112] As therapeutic approaches for type II diabetes continue to evolve and improve, the goal of treatments for type II diabetes related disorders is to intervene even before clinical signs (e.g., impaired glucose tolerance, or IGT) first manifest. Thus, genetic markers associated with susceptibility to type II diabetes prove useful for early diagnosis, prevention and treatment of type II diabetes.
[0113] As type II diabetes preventative and treatment information can be specifically targeted to subjects in need thereof (e.g., those at risk of developing type II diabetes or those that have early stages of type II diabetes), provided herein is a method for preventing or reducing the risk of developing type II diabetes in a subject, which comprises: (a) detecting the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying a subject with a predisposition to type II diabetes, whereby the presence of the polymorphic variation is indicative of a predisposition to type II diabetes in the subject; and (c) if such a predisposition is identified, providing the subject with information about methods or products to prevent or reduce type II diabetes or to delay the onset of type II diabetes. Also provided is a method of targeting information or advertising to a subpopulation of a human population based on the subpopulation being genetically predisposed to a disease or condition, which comprises: (a) detecting the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) identifying the subpopulation of subjects in which the polymorphic variation is associated with type II diabetes; and (c) providing information only to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition.
[0114] Pharmacogenomics methods also may be used to analyze and predict a response to a type II diabetes treatment or a drug. For example, if pharmacogenomics analysis indicates a likelihood that an individual will respond positively to a type II diabetes treatment with a particular drug, the drug may be administered to the individual. Conversely, if the analysis indicates that an individual is likely to respond negatively to treatment with a particular drug, an alternative course of treatment may be prescribed. A negative response may be defined as either the absence of an efficacious response or the presence of toxic side effects. The response to a therapeutic treatment can be predicted in a background study in which subjects in any of the following populations are genotyped: a population that responds favorably to a treatment regimen, a population that does not respond significantly to a treatment regimen, and a population that responds adversely to a treatment regiment (e.g., exhibits one or more side effects). These populations are provided as examples and other populations and subpopulations may be analyzed. Based upon the results of these analyses, a subject is genotyped to predict whether he or she will respond favorably to a treatment regimen, not respond significantly to a treatment regimen, or respond adversely to a treatment regimen. In an embodiment, a response to metformin is predicted based upon the genotype of a nucleic acid sample from a subject. In specific embodiments, detecting the presence of a cytosine at a position corresponding to position 50104 in SEQ ID NO: 1 in a nucleic acid sample is predictive of the subject not being responsive to metformin, and the drug typically is not prescribed to a subject having such a genotype.
[0115] The tests described herein also are applicable to clinical drug trials. One or more polymorphic variants indicative of response to an agent for treating type II diabetes or to side effects to an agent for treating type II diabetes may be identified using the methods described herein. Thereafter, potential participants in clinical trials of such an agent may be screened to identify those individuals most likely to respond favorably to the drug and exclude those likely to experience side effects. In that way, the effectiveness of drug treatment may be measured in individuals who respond positively to the drug, without lowering the measurement as a result of the inclusion of individuals who are unlikely to respond positively in the study and without risking undesirable safety problems.
[0116] Thus, another embodiment is a method of selecting an individual for inclusion in a clinical trial of a treatment or drug comprising the steps of: (a) obtaining a nucleic acid sample from an individual; (b) determining the identity of a polymorphic variation which is associated with a positive response to the treatment or the drug, or at least one polymorphic variation which is associated with a negative response to the treatment or the drug in the nucleic acid sample, and (c) including the individual in the clinical trial if the nucleic acid sample contains said polymorphic variation associated with a positive response to the treatment or the drug or if the nucleic acid sample lacks said polymorphic variation associated with a negative response to the treatment or the drug. In addition, the methods described herein for selecting an individual for inclusion in a clinical trial of a treatment or drug encompass methods with any further limitation described in this disclosure, or those following, specified alone or in any combination. The polymorphic variation may be in a sequence selected individually or in any combination from the group consisting of (i) a nucleotide sequence of SEQ ID NO: 1-20; (ii) a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20; (iii) a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ID NO: 1-20, or a nucleotide sequence about 90% or more identical to a nucleotide sequence of SEQ ID NO: 1-20; and (iv) a fragment of a polynucleotide sequence of (i), (ii), or (iii) comprising the polymorphic site. The including step (c) optionally comprises- administering the drug or the treatment to the individual if the nucleic acid sample contains the polymorphic variation associated with a positive response to the treatment or the drug and the nucleic acid sample lacks said biallelic marker associated with a negative response to the treatment or the drug.
[0117] Also provided herein is a method of partnering between a diagnostic/prognostic testing provider and a provider of a consumable product, which comprises: (a) the diagnostic/prognostic testing provider detects the presence or absence of a polymorphic variation associated with type II diabetes at a polymorphic site in a nucleotide sequence in a nucleic acid sample from a subject; (b) the diagnostic/prognostic testing provider identifies the subpopulation of subjects in which the polymorphic variation is associated with type II diabetes; (c) the diagnostic/prognostic testing provider forwards information to the subpopulation of subjects about a particular product which may be obtained and consumed or applied by the subject to help prevent or delay onset of the disease or condition; and (d) the provider of a consumable product forwards to the diagnostic test provider a fee every time the diagnostic/prognostic test provider forwards information to the subject as set forth in step (c) above. Compositions Comprising Diabetes-Directed Molecules
[0118] Featured herein is a composition comprising a cell from a subject having type II diabetes or at risk of type II diabetes and one or more molecules specifically directed and targeted to a nucleic acid comprising a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence or amino acid sequence. Such directed molecules include, but are not limited to, a compound that binds to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence or amino acid sequence referenced herein; a nucleic acid that hybridizes to a SLC22A 1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid under conditions of high stringency; a RNAi or siRNA molecule having a strand complementary to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence; an antisense nucleic acid complementary to an RNA encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence; a ribozyme that hybridizes to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLI3 nucleotide sequence; a nucleic acid aptamer that specifically binds a polypeptide encoded by SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLL3 nucleotide sequence; and an antibody that specifically binds to a polypeptide encoded by SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequence or binds to a nucleic acid having such a nucleotide sequence. In specific embodiments, the diabetes directed molecule interacts with a nucleic acid or polypeptide variant associated with diabetes, such as variants referenced herein. In other embodiments, the diabetes directed molecule interacts with a polypeptide involved in a signal pathway of a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, FRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence, or a nucleic acid comprising such a nucleotide sequence.
[0119] Compositions sometimea include an adjuvant known to stimulate an immune response, and in certain embodiments, an adjuvant that stimulates a T-cell lymphocyte response. Adjuvants are known, including but not limited to an aluminum adjuvant (e.g., aluminum hydroxide); a cytokine adjuvant or adjuvant that stimulates a cytokine response (e.g., interleukin (IL)-12 and/or γ- interferon cytokines); a Freund-type mineral oil adjuvant emulsion (e.g., Freund's complete or incomplete adjuvant); a synthetic lipoid compound; a copolymer adjuvant (e.g., TitreMax); a saponin; Quil A; a liposome; an oil-in-water emulsion (e.g., an emulsion stabilized by Tween SO and pluronic polyoxyethlene/polyoxypropylene block copolymer (Syntex Adjuvant Formulation); TitreMax; detoxified endotoxin (MPL) and mycobacterial cell wall components (TDW, CWS) in 2% squalene (Ribi Adjuvant System)); a muramyl dipeptide; an immune-stimulating complex (ISCOM, e.g., an Ag-modified saponin/cholesterol micelle that forms stable cage-like structure); an aqueous phase adjuvant that does not have a depot effect (e.g., Gerbu adjuvant); a carbohydrate polymer (e.g., AdjuPrime); L-tyrosine; a manide-oleate compound (e.g., Montanide); an ethylene- vinyl acetate copolymer (e.g., Elvax 40Wl, 2); or lipid A, for example. Such compositions are useful for generating an immune response against a diabetes directed molecule (e.g., an HLA- binding subsequence within a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence). In such methods, a peptide having an amino acid subsequence of a polypeptide encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleotide sequence is delivered to a subject, where the subsequence binds to an HLA molecule and induces a CTL lymphocyte response. The peptide sometimes is delivered to the subject as an isolated peptide or as a minigene in a plasmid that encodes the peptide. Methods for identifying HLA-binding subsequences in such polypeptides are known (see e.g., publication WO02/20616 and PCT application US98/01373 for methods of identifying such sequences).
[0120] The cell may be in a group of cells cultured in vitro or in a tissue maintained in vitro or present in an animal in vivo (e.g., a rat, mouse, ape or human). In certain embodiments, a composition comprises a component from a cell such as a nucleic acid molecule (e.g., genomic DNA), a protein mixture or isolated protein, for example. The aforementioned compositions have utility in diagnostic, prognostic and pharmacogenomic methods described previously and in diabetes therapeutics described hereafter. Certain diabetes directed molecules are described in greater detail below.
Compounds
[0121] Compounds can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; peptoid libraries (libraries of molecules having the functionalities of peptides, but with a novel, non-peptide backbone which are resistant to enzymatic degradation but which nevertheless remain bioactive (see, e.g., Zuckermann et al, J. Med. Chem.37: 2678-85 (1994)); spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; "one-bead one-compound" library methods; and synthetic library methods using affinity chromatography selection. Biological library and peptoid library approaches are typically limited to peptide libraries, while the other approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds (Lam, Anticancer Drug Des. 12: 145, (1997)). Examples of methods for synthesizing molecular libraries are described, for example, in DeWitt et al, Proc. Natl. Acad. Sci. U.S.A. 90: 6909 (1993); Erb et al, Proc. Natl. Acad. Sci. USA 91: 11422 (1994); Zuckermann et al, J. Med. Chem. 37: 2678 (1994); Cho et al, Science 261: 1303 (1993); Carrell et al, Angew. Chem. Int. Ed. Engl. 33: 2059 (1994); Carell et al, Angew. Chem. Int. Ed. Engl. 33: 2061 (1994); and in Gallop et al, J. Med. Chem. 37: 1233 (1994).
[0122] Libraries of compounds may be presented in solution {e.g., Houghten, Biotechniques 13: 412-421 (1992)), or on beads (Lam, Nature 354: 82-84 (1991)), chips (Fodόf, Nature 364: 555- 556 (1993)), bacteria or spores (Ladner, United States Patent No. 5,223,409), plasmids (Cull et al, Proc. Natl. Acad. Sci. USA 89: 1865-1869 (1992)) or on phage (Scott and Smith, Science 249: 386- 390 (1990); Devlin, Science 249: 404-406 (1990); Cwirla et al, Proc. Natl. Acad. Sci. 87: 6378- 6382 (1990); Felici, J. MoI. Biol. 222: 301-310 (1991); Ladner supra.).
[0123} A compound sometimes alters expression and sometimes alters activity of a polypeptide target and may be a small molecule. Small molecules include, but are not limited to, peptides, peptidomimetics {e.g., peptoids), amino acids, amino acid analogs, polynucleotides, polynucleotide analogs, nucleotides, nucleotide analogs, organic or inorganic compounds {i.e., including heteroorganic and organometallic compounds) having, a molecular weight less than about 10,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 5,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 1,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 500 grams per mole, and salts, esters, and other pharmaceutically acceptable forms of such compounds.
Antisense Nucleic Acid Molecules, Ribozymes, RNAi, siRNA and Modified Nucleic Acid Molecules
[0124] An "antisense" nucleic acid refers to a nucleotide sequence complementary to a "sense" nucleic acid encoding a polypeptide, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mKNA sequence. The antisense nucleic acid can be complementary to an entire coding strand {e.g., SEQ ID NO: 8-16}, or to a portion thereof or a substantially identical sequence thereof. In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence {e.g., 5' and 3' untranslated regions in SEQ ID NO: 1-9).
[0125] An antisense nucleic acid can be designed such that it is complementary to the entire coding region of an mRNA encoded by a nucleotide sequence {e.g., SEQ ID NO: 1-20), and often the antisense nucleic acid is an oligonucleotide antisense to only a portion of a coding or noncoding region of the mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of the mRNA, e.g., between the -10 and +10 regions of the target gene nucleotide sequence of interest. An antisense oligonucleotide can be, for example, about 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, or more nucleotides in length. The antisense nucleic acids, which include the ribozymes described hereafter, can be designed to target a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAM3 or GLB nucleotide sequence, often a variant associated with diabetes, or a substantially identical sequence thereof. Among the variants, minor alleles and major alleles can be targeted, and those associated with a higher risk of diabetes are often designed, tested, and administered to subjects.
[0126] An antisense nucleic acid can be constructed using chemical synthesis and enzymatic ligation reactions using standard procedures. For example, an antisense nucleic acid {e.g., an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides, or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used. Antisense nucleic acid also can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation {i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).
[0127] When utilized as therapeutics, antisense nucleic acids typically are administered to a subject {e.g., by direct injection at a tissue site) or generated in situ such that they hybridize with or bind to cellular niRNA and/or genomic DNA encoding a polypeptide and thereby inhibit expression of the polypeptide, for example, by inhibiting transcription and/or translation. Alternatively, antisense nucleic acid molecules can be modified to target selected cells and then are administered systemically. For systemic administration, antisense molecules can be modified such that they specifically bind to receptors, or antigens expressed on a selected cell surface, for example, by linking antisense nucleic acid molecules to peptides or antibodies which bind to cell surface receptors or antigens. Antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. Sufficient intracellular concentrations of antisense molecules, are achieved by incorporating a strong promoter, such as a pol II or pol III promoter, in the vector construct.
[0128] Antisense nucleic acid molecules sometimes, are *-anomeric nucleic acid molecules. An *-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual *-units, the strands run parallel to each other (Gaultier et ah, Nucleic Acids. Res. 15: 6625-6641 (1987)). Antisense nucleic acid molecules can also comprise a 2'-o-methylribonucleotide (Inoue et ah, Nucleic Acids Res. 15: 6131-6148 (1987)) or a chimeric RNA-DNA analogue (Inoue et ah, FEBS Lett. 215: 327-330 (1987)). Antisense nucleic acids sometimes are composed of DNA or PNA or any other nucleic acid derivatives described previously. [0129] In another embodiment, an antisense nucleic acid is a ribozyme. A ribozyme having specificity for a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence can include one or more sequences complementary to such a nucleotide sequence, and a sequence having a known catalytic region responsible for mRNA cleavage (see e.g., U.S. Pat. No. 5,093,246 or Haselhoff and Gerlach, Nature 334: 585-591 (1988)). For example, a derivative of a Tetrahymena L- 19 IVS RNA is sometimes utilized in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a mRNA (see e.g., Cech et al. U.S. Patent No. 4,987,071; and Cech et al. U.S. Patent No. 5,116,742). Also, target mRNA sequences can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules (see e.g., Bartel & Szostak, Science 261: 1411-1418 (1993)).
[0130] Diabetes directed molecules include in certain embodiments nucleic acids that can form triple helix structures with a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLIS nucleotide sequence or a substantially identical sequence thereof, especially one that includes a regulatory region that controls expression of a polypeptide. Gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of a nucleotide sequence referenced herein or a substantially identical sequence (e.g., promoter and/or enhancers) to form triple helical structures that prevent transcription of a gene in target cells (see e.g., Helene, Anticancer Drug Des. 6(6): 569-84 (1991); Helene et al, Ann. N.Y. Acad. Sci. 660: 27-36 (1992); and Maher, Bioassays 14(12): 807-15 (1992). Potential sequences that can be targeted for triple helix formation can be increased by creating a so-called "switchback" nucleic acid molecule. Switchback molecules are synthesized in an alternating 5'-3', 3'-5' manner, such that they base pair with first one strand of a duplex and then the other, eliminating the necessity for a sizeable stretch of either purines or pyrimidines to be present on one strand of a duplex.
[0131] Diabetes directed molecules include RNAi and siRNA nucleic acids. Gene expression may be inhibited by the introduction of double-stranded RNA (dsRNA), which induces potent and specific gene silencing, a phenomenon called RNA interference or RNAi. See, e.g., Fire et al, US Patent Number 6,506,559; Tuschl et al. PCT International Publication No. WO 01/75164; Kay et al. PCT International Publication No. WO 03/010180A1; or Bosher JM, Labouesse, Nat Cell Biol 2000 Feb;2(2):E31-6. This process has been improved by decreasing the size of the double- stranded RNA to 20-24 base pairs (to create small-interfering RNAs or siRNAs) that "switched off genes in mammalian cells without initiating an acute phase response, Le., a host defense mechanism that often results in cell death (see, e.g., Caplen et al. Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9742-7 and Elbashir et al. Methods 2002 Feb;26(2): 199-213). There is increasing evidence of post-transcriptional gene silencing by RNA interference (RNAi) for inhibiting targeted expression in mammalian cells at the mRNA level, in human cells. There is additional evidence of effective methods for inhibiting the proliferation and migration of tumor cells in human patients, and for inhibiting metastatic cancer development (see, e.g., U.S. Patent Application No. US2001000993183; Caplen et al. Proc Natl Acad Sci U S A; and Abderrahmani et al. Mόl Cell Biol 2001 Nov21(21):7256-67).
[0132] An "siRNA" or "KNAi" refers to a nucleic acid that forms a double stranded RNA and has the ability to reduce or inhibit expression of a gene or target gene when the siRNA is delivered to or expressed in the same cell as the gene or target gene. "siRNA" refers to short double-stranded RNA formed by the complementary strands. Complementary portions of the siRNA that hybridize to form the double stranded molecule often have substantial or complete identity to the target molecule sequence. In one embodiment, an siRNA refers to a nucleic acid that has substantial or complete identity to a target gene and forms a double stranded siRNA.
[0133] When designing the siRNA molecules, the targeted region often is selected from a given DNA sequence beginning 50 to 100 nucleotides downstream of the start codon. See, e.g., Elbashir et al,. Methods 26:199-213 (2002). Initially, 5' or 3' UTRs and regions nearby the start codon were avoided assuming that UTR-binding proteins and/or translation initiation complexes may interfere with binding of the siRNP or RISC endonuclease complex. Sometimes regions of the target 23 nucleotides in length conforming to the sequence motif AA(Nl 9)TT (N, an nucleotideX and regions with approximately 30% to 70% G/C-content (often about 50% G/C-content) often are selected. If no suitable sequences are found, the search often is extended using the motif NA(N21). The sequence of the sense siRNA sometimes corresponds to (N 19) TT or N21 (position 3 to 23 of the 23 -nt motif), respectively. In the latter case, the 3' end of the sense siRNA often is converted to TT. The rationale for this sequence conversion is to generate a symmetric duplex with respect to the sequence composition of the sense and antisense 3' overhangs. The antisense siRNA is synthesized as the complement to position 1 to 21 of the 23 -nt motif. Because position 1 of the 23- nt motif is not recognized sequence-specifically by the antisense siRNA, the 3 '-most nucleotide residue of the antisense siRNA can be chosen deliberately. However, the penultimate nucleotide of the antisense siRNA (complementary to position 2 of the 23 -nt motif) often is complementary to the targeted sequence. For simplifying chemical synthesis, TT often is utilized. siRNAs corresponding to the target motif NAR(N 17)YNN, where R is purine (A3G) and Y is pyrimidine (C,U), often are selected. Respective 21 nucleotide sense and antisense siRNAs often begin with a purine nucleotide and can also be expressed from pol III expression vectors without a change in targeting site. Expression of RNAs from pol III promoters often is efficient when the first transcribed nucleotide is a purine. [0134] The sequence of the siRNA can correspond to the full length target gene, or a subsequence thereof. Often, the siRNA is about 15 to about 50 nucleotides in length (e.g., each complementary sequence of the double stranded siRNA is 15-50 nucleotides in length, and the double stranded siRNA is about 15-50 base pairs in length, sometimes about 20-30 nucleotides in length or about 20-25 nucleotides in length, e.g., 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length. The siRNA sometimes is about 21 nucleotides in length. Methods of using siRNA are well known in the art, and specific siRNA molecules may be purchased from a number of companies including Dharmacon Research, Inc. An siRNA molecule sometimes is of a different chemical composition as compared to native RNA that imparts increased stability in cells (e.g., decreased susceptibility to degradation), and sometimes includes one or more modifications in siSTABLE RNA described at the http address www.dharmacon.com.
[0135} Antisense, ribozyme, RNAi and siRNA nucleic acids can be altered to form modified nucleic acid molecules. The nucleic acids can be altered at base moieties, sugar moieties or phosphate backbone moieties to improve stability, hybridization, or solubility of the molecule. For example, the deoxyribose phosphate backbone of nucleic acid molecules can be modified to generate peptide nucleic acids (see Hyrup et ah, Bioorganic & Medicinal Chemistry 4 (1): 5-23 (1996)). As used herein, the terms "peptide nucleic acid" or "PNA" refers to a nucleic acid mimic such as a DNA mimic, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained. The neutral backbone of a PNA can allow for specific hybridization to DNA and RNA under conditions of low ionic strength. Synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described, for example, in Hyrup et ah, (1996) supra and Perry-O'Keefe et ah, Proc. Natl. Acad. Sci. 93: 14670-675 (1996).
[0136] PNA nucleic acids can be used in prognostic, diagnostic, and therapeutic applications. For example, PNAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, for example, inducing transcription or translation arrest or inhibiting replication. PNA nucleic acid molecules can also be used in the analysis of single base pair mutations in a gene, (e.g., by PNA-directed PCR clamping); as "artificial restriction enzymes" when used in combination with other enzymes, (e.g., Sl nucleases (Hyrup (1996) supra)); or as probes or primers for DNA sequencing or hybridization (Hyrup et ah, (1996) supra; Perry-O'Keefe supra).
[0137] In other embodiments, oligonucleotides may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across cell membranes (see e.g., Letsinger et al., Proc. Natl. Acad. Sci. USA 86: 6553-6556 (1989); Lemaitre et ah, Proc. Natl. Acad. Sci. USA 84: 648-652 (1987); PCT Publication No. W088/09810) or the blood-brain barrier (see, e.g., PCT Publication No. W089/10134). In addition, oligonucleotides can be modified with hybridization-triggered cleavage agents (See, e.g., Krol et al., Bio-Techniques 6: 958-976 (1988)) or intercalating agents. (See, e.g., Zon, Pharm, Res. 5: 539-549 (1988) ). To this end, the oligonucleotide may be conjugated to another molecule, (e.g., a peptide, hybridization triggered cross-linking agent, transport agent, or hybridization-triggered cleavage agent).
[0138] Also included herein are molecular beacon oligonucleotide primer and probe molecules having one or more regions complementary to a SLC22A1, TTN, LOCI 12609, TKPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence or a substantially identical sequence thereof, two complementary regions one having a fluorophore and one a quencher such that the molecular beacon is useful for quantifying the presence of the nucleic acid in a sample. Molecular beacon nucleic acids are described, for example, in Lizardi et ah, U.S. Patent No. 5,854,033; Nazarenko et al, U.S. Patent No. 5,866,336, and Livak et al, U.S. Patent 5,876,930.
Antibody
[0139] The term "antibody" as used herein refers to an immunoglobulin molecule or immunologically active portion thereof, i.e., an antigen-binding portion. Examples of immunologically active portions of immunoglobulin molecules include F(ab) and F(ab')2 fragments which can be generated by treating the antibody with an enzyme such as pepsin. An antibody sometimes is a polyclonal, monoclonal, recombinant {e.g., a chimeric or humanized), fully human, non-human (e.g., murine), or a single chain antibody. An antibody may have effector function and can fix complement, and is sometimes coupled to a toxin or imaging agent.
Antibodies
[0140] A full-length polypeptide or antigenic peptide fragment encoded by a nucleotide sequence referenced herein can be uaed as an immunogen or can be used to identify antibodies made with other immunogens, e.g., cells, membrane preparations, and the like. An antigenic peptide often includes at least 8 amino acid residues of the amino acid sequences encoded by a nucleotide sequence referenced herein, or substantially identical sequence thereof, and encompasses an epitope. Antigenic peptides sometimes include 10 or more amino acids, 15 or more amino acids, 20 or more amino acids, or 30 or more amino acids. Hydrophilic and hydrophobic fragments of polypeptides sometimes are used as immunogens.
[0141] Epitopes encompassed by the antigenic peptide are regions located on the surface of the polypeptide (e.g., hydrophilic regions) as well as regions with high antigenicity. For example, an Emini surface probability analysis of the human polypeptide sequence can be used to indicate the regions that have a particularly high probability of being localized to the surface of the polypeptide and are thus likely to constitute surface residues useful for targeting antibody production. The antibody may bind an epitope on any domain or region on polypeptides described herein.
[0142] Also, chimeric, humanized, and completely human antibodies are useful for applications which include repeated administration to subjects. Chimeric and humanized monoclonal antibodies, comprising both human and non-human portions, can be made using standard recombinant DNA techniques. Such chimeric and humanized monoclonal antibodies can be produced by recombinant DNA techniques known in the art, for example using methods described in Robinson et al International Application No. PCT/US86/02269; Akira, et al European Patent Application 184,187; Taniguchi, M., European Patent Application 171,496; Morrison et al European Patent Application 173,494; Neuberger et al PCT International Publication No. WO 86/01533; Cabilly et al U.S. Patent No. 4,816,567; Cabilly et al European Patent Application 125,023; Better et al, Science 240: 1041-1043 (1988>; Liu et al, Proc. Natl. Acad. Sci. USA 84: 3439-3443 (1987); Liu et al, J. Immunol. 139: 3521-3526 (1987); Sun et al, Proc. Natl. Acad. Sci. USA 84: 214-218 (1987); Nishimura et al, Cane. Res. 47: 999-1005 (1987); Wood et al, Nature 314: 446-449 (1985}; and Shaw et al, J. Natl. Cancer Inst. 80: 1553-1559 (1988}; Morrison, S. L., Science 229: 1202-1207 (1985); Oi et al, BioTechniques 4: 214 (1986); Winter U.S. Patent 5,225,539; Jones etal, Nature 321: 552-525 (1986); Verhoeyan etal, Science 239: 1534; and Beidler et al, J. Immunol. 141: 4053-4060 (1988).
[0143] Completely human antibodies are particularly desirable for therapeutic treatment of human patients. Such antibodies can be produced using transgenic mice that are incapable of expressing endogenous immunoglobulin heavy and light chains genes, but which can express human heavy and light chain genes. See, for example, Lonberg and Huszar, Int. Rev. Immunol. 13: 65-93 (1995); and U.S. Patent Nos. 5,625,126; 5,633,425; 5,569,825; 5,661,016; and 5,545,806. In addition, companies such as Abgenix, Inc. (Fremont, CA) and Medarex, Inc. (Princeton, NJ}, can be engaged to provide human antibodies directed against a selected antigen using technology similar to that described above. Completely human antibodies that recognize a selected epitope also can be generated using a technique referred to as "guided selection." In this approach a selected non- human monoclonal antibody {e.g., a murine antibody) is used to guide the selection of a completely human antibody recognizing the same epitope. This technology is described for example by Jespers et al, Bio/Technology 12: 899-903 (1994).
[0144] An antibody can be a single chain antibody. A single chain antibody (scFV) can be engineered (see, e.g., Colcher et al, Ann. N Y Acad. Sci. 880: 263-80 (1999); and Reiter, Clin. Cancer Res. 2: 245-52 (1996)). Single chain antibodies can be dimerized or multimerized to generate multivalent antibodies having specificities for different epitopes of the same target polypeptide. [0145] Antibodies also may be selected or modified so that they exhibit reduced or no ability to bind an Fc receptor. For example, an antibody may be an isotype or subtype, fragment or other mutant, which does not support binding to an Fc receptor (e.g., it has a mutagenized or deleted Fc receptor binding region).
[0146] Also, an antibody (or fragment thereof) may be conjugated to a therapeutic moiety such as a cytotoxin, a therapeutic agent or a radioactive metal ion. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells. Examples include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1 dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. Therapeutic agents include, but are not limited to, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-ftuorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thiotepa chlorambucil, melphalan, carmustine (BCNU) and lomustine (CCNU), cyclophosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin}, bleomycin, mithramycin, and anthramycin (AMC)), and anti¬ mitotic agents (e.g., vincristine and vinblastine).
[0147] Antibody conjugates can be used for modifying a given biological response. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, γ-interferon, α-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator; or, biological response modifiers such as, for example, lymphokines, interleukin-1 ("IL-I"), interleukin-2 ("IL-2"), interleukin-6 ("IL-6"), granulocyte macrophage colony stimulating factor ("GM-CSF"), granulocyte colony stimulating factor ("G-CSF"), or other growth factors. Also, an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Patent No. 4,676,980, for example.
[0148] An antibody (e.g., monoclonal antibody) can be used to isolate target polypeptides by standard techniques, such as affinity chromatography or immunoprecipitation. Moreover, an antibody can be used to detect a target polypeptide (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the polypeptide. Antibodies can be used diagnostically to monitor polypeptide levels in tissue as part of a clinical testing procedure, e.g., to determine the efficacy of a given treatment regimen. Detection can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance (i.e., antibody labeling). Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include 1251, 1311, 35S or 3H. Also, an antibody can be utilized as a test molecule for determining whether it can treat diabetes, and as a therapeutic for administration to a subject for treating diabetes,
[0149] An antibody can be made by immunizing with a purified antigen, or a fragment thereof, e.g., a fragment described herein, a membrane associated antigen, tissues, e.g., crude tissue preparations, whole cells, preferably living cells, lysed cells, or cell fractions.
[0150] Included herein are antibodies which bind only a native polypeptide, only denatured or otherwise non-native polypeptide, or which bind both, as well as those having linear or conformational epitopes. Conformational epitopes sometimes can be identified by selecting antibodies that bind to native but not denatured polypeptide. Also featured are antibodies that specifically bind to a polypeptide variant associated with diabetes.
Methods for Identifying Candidate Therapeutics for Treating Type II Diabetes [0151] Current therapies for the treatment of type II diabetes have limited efficacy, limited tolerability and significant mechanism-based side effects, including weight gain and hypoglycemia. Few of the available therapies adequately address underlying defects such as obesity and insulin resistance (Moller D. Nature. 414:821-827 (2001)). Current therapeutic approaches were largely developed in the absence of defined molecular targets or even a solid understanding of disease pathogenesis. Therefore, provided are methods of identifying candidate therapeutics that target biochemical pathways related to the development of diabetes.
[0152] Thus, featured herein are methods for identifying a candidate therapeutic for treating type II diabetes. The methods comprise contacting a test molecule with a target molecule in a system. A "target molecule" as used herein refers to a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid, a substantially identical nucleic acid thereof, or a fragment thereof, and an encoded polypeptide of the foregoing. The methods also comprise determining the presence or absence of an interaction between the test molecule and the target molecule, where the presence of an interaction between the test molecule and the nucleic acid or polypeptide identifies the test molecule as a candidate type II diabetes therapeutic. The interaction between the test molecule and the target molecule may be quantified.
[0153] Test molecules and candidate therapeutics include, but are not limited to, compounds, antisense nucleic acids, siRNA molecules, ribozymes, polypeptides or proteins encoded by a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS or GLB nucleotide sequence, or a substantially identical sequence or fragment thereof, and immunotherapeutics. (e.g., antibodies and HLA-presented polypeptide fragments). A test molecule or candidate therapeutic may act as a modulator of target molecule concentration or target molecule function in a system. A "modulator" may agonize {i.e., up-regulates) or antagonize (i.e., down-regulates) a target molecule concentration partially or completely in a system by affecting such cellular functions as DNA replication and/or DNA processing {e.g., DNA methylation or DNA repair), RNA transcription and/or RNA processing {e.g., removal of intronic sequences and/or translocation of spliced mRNA from the nucleus), polypeptide production (e.g., translation of the polypeptide from mRNA), and/or polypeptide post-translational modification (e.g., glycosylation, phosphorylation, and proteolysis, of pro-polypeptides). A modulator may also agonize or antagonize a biological function of a target molecule partially or completely, where the function may include adopting a certain structural conformation, interacting with one or more binding partners, ligand binding, catalysis (e.g., phosphorylation, dephosphorylation, hydrolysis, methylation, and isomerization), and an effect upon a cellular event (e.g., effecting progression of type II diabetes). In certain embodiments, a candidate therapeutic increases glucose uptake in cells of a subject (e.g., in certain cells of the pancreas).
[0154] As used herein, the term "system" refers to a cell free in vitro environment and a cell- based environment such as a collection of cells, a tissue, an organ, or an organism. A system is "contacted" with a test molecule in a variety of manners, including adding molecules in solution and allowing them to interact with one another by diffusion, cell injection, and any administration routes in an animal. As used herein, the term "interaction" refers to an effect of a test molecule on test molecule, where the effect sometimes is binding between the test molecule and the target molecule, and sometimes is an observable change in cells, tissue, or organism.
[0155] There are many standard methods for detecting the presence or absence of interaction between a test molecule and a target molecule. For example, titrametric, acidimetric, radiometric, NMR, monolayer, polarographic, spectrophotometric, fluorescent, and ESR assays probative of a target molecule interaction may be utilized.
[0156] Test molecule/target molecule interactions can be detected and/or quantified using assays known in the art. For example, an interaction can be determined by labeling the test molecule and/or the target molecule, where the label is covalently or non-covaleiitly attached to the test molecule or target molecule. The label is sometimes a radioactive molecule such as 1251, 131I, 35S or Η, which can be detected by direct counting of radioemission or by scintillation counting. Also, enzymatic labels such as horseradish peroxidase, alkaline phosphatase, or luciferase may be utilized where the enzymatic label can be detected by determining conversion of an appropriate substrate to product. In addition, presence or absence of an interaction can be determined without labeling. For example, a microphysiometer (e.g., Cytosensor) is an analytical instrument that measures the rate at which a cell acidifies its environment using a light-addressable potentiometric sensor (LAPS). Changes in this acidification rate can be used as an indication of an interaction between a test molecule and target molecule (McConnell, H. M. et al, Science 257: 1906-1912 (1992».
[0157] In cell-based systems, cells typically include a SLC22A1, TTN, LOC112609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid, an encoded polypeptide, or substantially identical nucleic acid or polypeptide thereof, and are often of mammalian origin, although the cell can be of any origin. Whole cells, cell homogenates, and cell fractions (e.g., cell membrane fractions) can be subjected to analysis. Where interactions between a test molecule with a target polypeptide are monitored, soluble and/or membrane bound forms of the polypeptide may be utilized. Where membrane-bound forms of the polypeptide are used, it may be desirable to utilize a solubilizing agent. Examples of such solubilizing agents include non-ionic detergents such as n- octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide, decanoyl-
N-methylglucamide, Triton® X-IOO, Triton® X-114, Thesit®, Isotridecypoly(ethylene glycol ether)n, 3-[(3-cholamidopropyl)dimethylamminio]-l-propane sulfonate (CHAPS), 3-[(3- cholamidopropyl)dimethylamminio]-2-hydroxy-l -propane sulfonate (CHAPSO), or N-dodecyl- N,N-dimethy 1-3 -ammonio- 1 -propane sulfonate.
[0158] An interaction between a test molecule and target molecule also can be detected by monitoring fluorescence energy transfer (FET) (see, e.g., Lakowicz et at, U.S. Patent No. 5,631,169; Stavrianopoulos et al. U.S. Patent No. 4,868,103). A fluorophore label on a first, "donor" molecule is selected such that its emitted fluorescent energy will be absorbed by a fluorescent label on a second, "acceptor" molecule, which in turn is able to fluoresce due to the absorbed energy. Alternately, the "donor" polypeptide molecule may simply utilize the natural fluorescent energy of tryptophan residues. Labels are chosen that emit different wavelengths of light, such that the "acceptor" molecule label may be differentiated from that of the "donor". Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, the spatial relationship between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the "acceptor" molecule label in the assay should be maximal. An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art {e.g., using a fluorimeter).
[0159] In another embodiment, determining the presence or absence of an interaction between a test molecule and a target molecule can be effected by monitoring surface plasmon resonance {see, e.g., Sjolander & Urbaniczk, Anal. Chem. 63: 2338-2345 (1991) and Szabo et al, Curr. Opin. Struct. Biol. 5: 699-705 (1995)). "Surface plasmon resonance" or "biomolecular interaction analysis (BIA)" can be utilized to detect biospecific interactions in real time, without labeling any of the interactants {e.g., BIAcore}. Changes in the mass at the binding surface (indicative of a binding event) result in alterations of the refractive index of light near the surface (the optical phenomenon of surface plasmon resonance (SPR)X resulting in a detectable signal which can be used as an indication of real-time reactions between biological molecules.
[0160] In another embodiment, the target molecule or test molecules are anchored to a solid phase, facilitating the detection of target molecule/test molecule complexes and separation of the complexes from free, uncomplexed molecules. The target molecule or test molecule is immobilized to the solid support. In an embodiment, the target molecule is anchored to a solid surface, and the test molecule, which is not anchored, can be labeled, either directly or indirectly, with detectable labels discussed herein.
[0161] It may be desirable to immobilize a target molecule, an anti-target molecule antibody, and/or test molecules to facilitate separation of target molecule/test molecule complexes from uncomplexed forms, as well as to accommodate automation of the assay. The attachment between a test molecule and/or target molecule and the solid support may be covalent or non-covalent {see, e.g., U.S. Patent No. 6,022,688 for non-covalent attachments). The solid support may be one or more surfaces of the system, such as one or more surfaces in each well of a microtiter plate, a surface of a silicon wafer, a surface of a bead {see, e.g., Lam, Nature 354: 82-84 (1991))- that is optionally linked to another solid support, or a channel in a microfluidic device, for example. Types of solid supports, linker molecules for covalent and non-covalent attachments to solid supports, and methods for immobilizing nucleic acids and other molecules to solid supports are well known {see, e.g., U.S. Patent Nos. 6,261,776; 5,900,481; 6,133,436; and 6,022,688; and WIPO publication WO 01/18234).
[0162] In an embodiment, target molecule may be immobilized to surfaces via biotin and streptavidin. For example, biotinylated target polypeptide can be prepared from biotin-NHS (N- hydroxy-succinimide) using techniques known in the art {e.g., biotinylation kit, Pierce Chemicals, Rockford, IL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). In another embodiment, a target polypeptide can be prepared as a fusion polypeptide. For example, glutathione-S-transferase/target polypeptide fusion can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, MO) or glutathione derivitized microtiter plates, which are then combined with a test molecule under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, or the matrix is immobilized in the case of beads, and complex formation is determined directly or indirectly as described above. Alternatively, the complexes can be dissociated from the matrix, and the level of target molecule binding or activity is determined using standard techniques.
[0163] In an embodiment, the non-immobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that a significant percentage of complexes formed will remain immobilized to the solid surface. The detection of complexes anchored on the solid surface can be accomplished in a number of manners. Where the previously non-immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the previously non-immobilized component is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface, e.g., by adding a labeled antibody specific for the immobilized component, where the antibody, in turn, can be directly labeled or indirectly labeled with, e.g., a labeled anti-Ig antibody.
[0164] In another embodiment, an assay is performed utilizing antibodies that specifically bind target molecule or test molecule but do not interfere with binding of the target molecule to the test molecule. Such antibodies can be derivitized to a solid support, and unbound target molecule may be immobilized by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.
[0165] Cell free assays also can be conducted in a liquid phase. In such an assay, reaction products are separated from unreacted components, by any of a number of standard techniques, including but not limited to: differential centrifugation (see, e.g., Rivas, G., and Minton, Trends Biochem SciAug;18(8): 284-7 (1993)); chromatography (gel filtration chromatography, ion- exchange chromatography); electrophoresis (see, e.g., Ausubel et al., eds. Current Protocols in Molecular Biology , J. Wiley: New York (1999)); and immunoprecipitation (see, e.g., Ausubel et al, eds., supra). Media and chromatographic techniques are known to one skilled in the art (see, e.g., Heegaard, JMoI. Recognit. Winter; 11(1-6): 141-8 (1998); Hage & Tweed, J. Chromatogr. B Biomed. ScL Appl. Oct 10; 699 (1-2): 499-525 (1997)). Further, fluorescence energy transfer may also be conveniently utilized, as described herein, to detect binding without further purification of the complex from solution. [0166] In another embodiment, modulators of target molecule expression are identified. For example, a cell or cell free mixture is contacted with a candidate compound and the expression of target mRNA or target polypeptide is evaluated relative to the level of expression of target mRNA or target polypeptide in the absence of the candidate compound. When expression of target mRNA or target polypeptide is greater in the presence of the candidate compound than in its absence, the candidate compound is identified as an agonist of target mRNA or target polypeptide expression. Alternatively, when expression of target mRNA or target polypeptide is less (e.g., less with statistical significance) in the presence of the candidate compound than in its absence, the candidate compound is. identified as an antagonist or inhibitor of target mRNA or target polypeptide expression. The level of target mRNA or target polypeptide expression can be determined by methods described herein.
[0167] In another embodiment, binding partners that interact with a target molecule are detected. The target molecules can interact with one or more cellular or extracellular macromolecules, such as polypeptides in vivo, and these interacting molecules are referred to herein as "binding partners." Binding partners can agonize or antagonize target molecule biological activity. Also, test molecules that agonize or antagonize interactions between target molecules and binding partners can be useful as therapeutic molecules as they can up-regulate or down-regulated target molecule activity in vivo and thereby treat type II diabetes.
[0168} Binding partners of target molecules can be identified by methods, known in the art. For example, binding partners may be identified by lysing cells and analyzing cell lysates by electrophoretic techniques. Alternatively, a two-hybrid assay or three-hybrid assay can be utilized (see, e.g., U.S. Patent No. 5,283,317; Zervos et al., Cell 72:223-232 (1993); Madura et al, J. Biol. Chem. 268: 12046-12054 (1993); Bartel et al, Biotechniques 14: 920-924 (1993); Iwabuchi et al., Oncogene 8: 1693-1696 (1993); and Brent WO94/10300). A two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. The assay often utilizes two different DNA constructs. In one construct, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid (sometimes referred to as the "bait") is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In another construct, a DNA sequence from a library of DNA sequences that encodes a potential binding partner (sometimes referred to as the "prey") is fused to a gene that encodes an activation domain of the known transcription factor. Sometimes, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 or GLB nucleic acid can be fused to the activation domain. If the "bait" and the "prey" molecules interact in vivo, the DNA- binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) which is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to identify the potential binding partner.
[0169] In an embodiment for identifying test molecules that antagonize or agonize complex formation between target molecules and binding partners, a reaction mixture containing the target molecule and the binding partner is prepared, under conditions and for a time sufficient to allow complex formation. The reaction mixture often is provided in the presence or absence of the test molecule. The test molecule can be included initially in the reaction mixture, or can be added at a time subsequent to the addition of the target molecule and its binding partner. Control reaction mixtures are incubated without the test molecule or with a placebo. Formation of any complexes between the target molecule and the binding partner then is detected. Decreased formation of a complex in the reaction mixture containing test molecule as compared to in a control reaction mixture indicates that the molecule antagonizes target molecule/binding partner complex formation. Alternatively, increased formation of a complex in the reaction mixture containing test molecule as. compared to in a control reaction mixture indicates that the molecule agonizes target molecule/binding partner complex formation. In another embodiment, complex formation of target molecule/binding partner can be compared to complex formation of mutant target molecule/binding partner (e.g., amino acid modifications in a target polypeptide). Such a comparison can be important in those cases where it is desirable to identify test molecules that modulate interactions of mutant but not non-mutated target gene products.
[0170] The assays can be conducted in a heterogeneous or homogeneous format. In heterogeneous assays, target molecule and/or the binding partner are immobilized to a solid phase, and complexes are detected on the solid phase at the end of the reaction. In homogeneous assays, the entire reaction is carried out in a liquid phase. In either approach, the order of addition of reactants can be varied to obtain different information about the molecules being tested. For example, test compounds that agonize target molecule/binding partner interactions can be identified by conducting the reaction in the presence of the test molecule in a competition format. Alternatively, test molecules that agonize preformed complexes, e.g., molecules with higher binding constants that displace one of the components from the complex, can be tested by adding the test compound to the reaction mixture after complexes have been formed.
[0171] In a heterogeneous assay embodiment, the target molecule or the binding partner is anchored onto a solid surface (e.g., a microtiter plate), while the non-anchored species is labeled, either directly or indirectly. The anchored molecule can be immobilized by non-covalent or covalent attachments. Alternatively, an immobilized antibody specific for the molecule to be anchored can be used to anchor the molecule to the solid surface. The partner of the immobilized species is exposed to the coated surface with or without the test molecule. After the reaction is complete, unreacted components are removed (e.g., by washing) such that a significant portion of any complexes formed will remain immobilized on the solid surface. Where the non-immobilized species is pre-labeled, the detection of label immobilized on the surface is indicative of complex. Where the non-immobilized species is not pre-labeled, an indirect label can be used to detect complexes anchored to the surface; e.g., by using a labeled antibody specific for the initially non- immobilized species. Depending upon the order of addition of reaction components, test compounds that inhibit complex formation or that disrupt preformed complexes can be detected.
[0172] In another embodiment, the reaction can be conducted in a liquid phase in the presence or absence of test molecule, where the reaction products are separated from unreacted components, and the complexes are detected (e.g., using an immobilized antibody specific for one of the binding components to anchor any complexes formed in solution, and a labeled antibody specific for the other partner to detect anchored complexes)-. Again, depending upon the order of addition of reactants to the liquid phase, test compounds that inhibit complex or that disrupt preformed complexes can be identified.
[0173] In an alternate embodiment, a homogeneous assay can be utilized. For example, a preformed complex of the target gene product and the interactive cellular or extracellular binding partner product is prepared. One or both of the target molecule or binding partner is labeled, and the signal generated by the label(s) is quenched upon complex formation (e.g., U.S. Patent No. 4,109,496 that utilizes this approach for immunoassays). Addition of a test molecule that competes with and displaces one of the species from the preformed complex will result in the generation of a signal above background. In this way, test substances that disrupt target molecule/binding partner complexes can be identified.
[0174] Candidate therapeutics for treating type II diabetes are identified from a group of test molecules that interact with a target molecule. Test molecules are normally ranked according to the degree with which they modulate (e.g., agonize or antagonize) a function associated with the target molecule (e.g., DNA replication and/or processing, RNA transcription and/or processing, polypeptide production and/or processing, and/or biological function/activity), and then top ranking modulators are selected. Also, pharmacogenomic information described herein can determine the rank of a modulator. The top 10% of ranked test molecules often are selected for further testing as candidate therapeutics, and sometimes the top 15%, 20%, or 25% of ranked test molecules are selected for further testing as candidate therapeutics. Candidate therapeutics typically are formulated for administration to a subject. Therapeutic Formulations
[0175] Formulations and pharmaceutical compositions typically include in combination with a pharmaceutically acceptable carrier one or more target molecule modulators. The modulator often is a test molecule identified as having an interaction with a target molecule by a screening method described above. The modulator may be a compound, an antisense nucleic acid, a ribozyme, an antibody, or a binding partner. Also, formulations may comprise a target polypeptide or fragment thereof in combination with a pharmaceutically acceptable carrier
[0176] As used herein, the term "pharmaceutically acceptable carrier" includes solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Supplementary active compounds can also be incorporated into the compositions. Pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration.
[0177] A pharmaceutical composition typically is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g. , intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
[0178] Oral compositions generally include an inert diluent or an edible carrier. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules, e.g., gelatin capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring. [0179] Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, NJ) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringability exists. It should be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like, hi many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.
[0180] Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
[0181] For administration by inhalation, the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
[0182] Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art. Molecules can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
[0183] In one embodiment, active molecules are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. Materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811.
[0184] It is advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
[0185] Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50. Molecules which exhibit high therapeutic indices are preferred. While molecules that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.
[0186] The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such molecules lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any molecules used in the methods described herein, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma may be measured, for example, by high performance liquid chromatography.
[0187] As defined herein, a therapeutically effective amount of protein or polypeptide (i.e., an effective dosage) ranges from about 0.001 to 30 mg/kg body weight, sometimes about 0.01 to 25 mg/kg body weight, often about 0.1 to 20 mg/kg body weight, and more often about 1 to 10 mg/kg, 2 to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight. The protein or polypeptide can be administered one time per week for between about 1 to 10 weeks, sometimes between 2 to 8 weeks, often between about 3 to 7 weeks, and more often for about 4, 5, or 6 weeks. The skilled artisan will appreciate that certain factors may influence the dosage and timing required to effectively treat a subject, including but not limited to the severity of the disease or disorder, previous treatments, the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of a protein, polypeptide, or antibody can include a single treatment or, preferably, can include a series of treatments.
[0188] With regard to polypeptide formulations, featured herein is a method for treating type II diabetes in a subject, which comprises contacting one or more cells in the subject with a first polypeptide, where the subject comprises a second polypeptide having one or more polymorphic variations associated with cancer, and where the first polypeptide comprises fewer polymorphic variations associated with cancer than the second polypeptide. The first and second polypeptides are encoded by a nucleic acid which comprises a nucleotide sequence in SEQ ID NO: 1-20; a nucleotide sequence which encodes a polypeptide consisting of an amino acid sequence encoded by a nucleotide sequence referenced in SEQ ID NO: 1-20; a nucleotide sequence which encodes a polypeptide that is 90% or more identical to an amino acid sequence encoded by a nucleotide sequence of SEQ ED NO: 1-20 and a nucleotide sequence 90% or more identical to a nucleotide sequence in SEQ ID NO: 1-20. The subject often is a human.
[0189] For antibodies, a dosage of 0.1 mg/kg of body weight (generally 10 mg/kg to 20 mg/kg) is often utilized. If the antibody is to act in the brain, a dosage of 50 mg/kg to 100 mg/kg is often appropriate. Generally, partially human antibodies and fully human antibodies have a longer half- life within the human body than other antibodies. Accordingly, lower dosages and less frequent administration is often possible. Modifications such as lipidation can be used to stabilize antibodies and to enhance uptake and tissue penetration (e.g., into the brain). A method for lipidation of antibodies is described by Cruikshank et al, J. Acquired Immune Deficiency Syndromes and Human Retrovirology 14:193 (1997).
[0190] Antibody conjugates can be used for modifying a given biological response, the drug moiety is not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumor necrosis factor, .alpha. -interferon, .beta. -interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator; or, biological response modifiers such as, for example, lymphokines, interleukin-1 ("IL-I"), interleukin-2 ("IL-2"), interleukin-6 ("IL-6"), granulocyte macrophage colony stimulating factor ("GM-CSF"), granulocyte colony stimulating factor ("G-CSF"), or other growth factors. Alternatively, an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Patent No. 4,676,980.
[0191] For compounds, exemplary doses include milligram or microgram amounts of the compound per kilogram of subject or sample weight, for example, about 1 microgram per kilogram to about 500 milligrams per kilogram, about 100 micrograms per kilogram to about 5 milligrams per kilogram, or about 1 microgram per kilogram to about 50 micrograms per kilogram. It is understood that appropriate doses of a small molecule depend upon the potency of the small molecule with respect to the expression or activity to be modulated. When one or more of these small molecules is to be administered to an animal {e.g., a human) in order to modulate expression or activity of a polypeptide or nucleic acid described herein, a physician, veterinarian, or researcher may, for example, prescribe a relatively low dose at first, subsequently increasing the dose until an appropriate response is obtained. In addition, it is understood that the specific dose level for any particular animal subject will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, gender, and diet of the subject, the time of administration, the route of administration, the rate of excretion, any drug combination, and the degree of expression or activity to be modulated.
[0192] With regard to nucleic acid formulations, gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration {see, e.g., U.S. Patent 5,328,470) or by stereotactic injection {see e.g., Chen et al, (1994) Proc. Natl Acad. ScL USA i?i:3054-3057). Pharmaceutical preparations of gene therapy vectors can include a gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells {e.g., retroviral vectors) the pharmaceutical preparation can include one or more cells which produce the gene delivery system. Examples of gene delivery vectors are described herein.
Therapeutic Methods
[0193] A therapeutic formulation described above can be administered to a subject in need of a therapeutic for inducing a desired biological response.. Therapeutic formulations can be administered by any of the paths described herein. With regard to both prophylactic and therapeutic methods of treatment, such treatments may be specifically tailored or modified, based on knowledge obtained from pharmacogenomic analyses described herein.
[0194] As used herein, the term "treatment" is defined as the application or administration of a therapeutic formulation to a subject, or application or administration of a therapeutic agent to an isolated tissue or cell line from a subject with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, improye or affect type II diabetes, symptoms of type II diabetes or a predisposition towards type II diabetes. A therapeutic formulation includes, but is not limited to, small molecules, peptides, antibodies, ribozymes and antisense oligonucleotides. Administration of a therapeutic formulation can occur prior to the manifestation of symptoms characteristic of type II diabetes, such that type II diabetes is prevented or delayed in its progression. The appropriate therapeutic composition can be determined based on screening assays described herein.
[0195] In related aspects, embodiments include methods of causing or inducing a desired biological response in an individual comprising the steps of: providing or administering to an individual a composition comprising a polypeptide described herein, or a fragment thereof, or a therapeutic formulation described herein, wherein said biological response is selected from the group consisting of: (a) modulating circulating (either blood, serum or plasma) levels (concentration) of glucose, wherein said modulating is preferably lowering; (b) increasing cell or - tissue sensitivity to insulin, particularly muscle, adipose, liver or brain; (c) inhibiting the progression from impaired glucose tolerance to insulin resistance; (d) increasing glucose uptake in skeletal muscle cells; (e) increasing glucose uptake in adipose cells; (f) increasing glucose uptake in neuronal cells; (g) increasing glucose uptake in red blood cells; (h) increasing glucose uptake in the brain; and (i) significantly reducing the postprandial increase in plasma glucose following a meal, particularly a high carbohydrate meal.
[0196] Ia other embodiments, a pharmaceutical or physiologically acceptable composition can be utilized as an insulin sensitizer, or can be used in: a method to improve insulin sensitivity in some persons with type II diabetes in combination with insulin therapy; a method to improve insulin sensitivity in some persons with type II diabetes without insulin therapy; or a method of treating individuals with gestational diabetes. Gestational diabetes refers to the development of diabetes in an individual during pregnancy, usually during the second or third trimester of pregnancy. In further embodiments, the pharmaceutical or physiologically acceptable composition can be used in a method of treating individuals with impaired fasting glucose (IFG). Impaired fasting glucose (IFG) is a condition in which fasting plasma glucose levels in an individual are elevated but not diagnostic of overt diabetes (i.e. plasma glucose levels of less than 126 mg/dl and greater than or equal to 110 mg/dl). [0197] In other embodiments, the pharmaceutical or physiologically acceptable composition can be used in a method of treating and preventing impaired glucose tolerance (IGT) in an individual. By providing therapeutics and methods for reducing or preventing IGT (i.e., for normalizing insulin resistance) the progression to type II diabetes can be delayed or prevented. Furthermore, by providing therapeutics and methods for reducing or preventing insulin resistance, provided are methods for reducing and/or preventing the appearance of Insulin-Resistance Syndrome (IRS). In further embodiments, the pharmaceutical or physiologically acceptable composition can be used in a method of treating a subject having polycystic ovary syndrome (PCOS). PCOS is among the most common disorders of premenopausal women, affecting 5-10% of this population. Insulin-sensitizing agents (e.g., troglitazone) have been shown to be effective in PCOS and that, in particular, the defects in insulin action, insulin secretion, ovarian steroidogenesis and fibrinolysis are improved (Ehrman et al. (1997) J Clin Invest 100:1230), such as in insulin- resistant humans. Accordingly, provided are methods for reducing insulin resistance, normalizing blood glucose thus treating and/or preventing PCOS.
[0198] In certain embodiments, the pharmaceutical or physiologically acceptable composition can be used in a method of treating a subject having insulin resistance, where a subject having insulin resistance is treated to reduce or cure the insulin resistance. As insulin resistance is also often associated with infections and cancer, preventing or reducing insulin resistance may prevent or reduce infections and cancer.
[0199] hi other embodiments, the pharmaceutical compositions and methods described herein are useful for: preventing the development of insulin resistance in a subject, e.g., those known to have an increased risk of developing insulin resistance; controlling blood glucose in some persons with type II diabetes in combination with insulin therapy; increasing cell or tissue sensitivity to insulin, particularly muscle, adipose, liver or brain; inhibiting or preventing the progression from impaired glucose tolerance to insulin resistance; improving glucose control of type II diabetes patients alone, without an insulin secretagogue or an insulin sensitizing agent; and administering a complementary therapy to type II diabetes patients to improve their glucose control in combination with an insulin secretagogue (preferably oral form) or an insulin sensitizing (preferably oral form) agent. In the latter embodiment, the oral insulin secretagogue sometimes is l,l-dimethyl-2-(2- morpholino phenyl)guanidine fumarate (BTS67582) or a sulphonylurea selected from tolbutamide, tolazamide, chlorpropamide, glibenclamide, glimepiride, glipizide and glidazide. The insulin sensitizing agent sometimes is selected from metformin, ciglitazone, troglitazone and pioglitazone.
[0200] Further embodiments include methods of administering a pharmaceutical or physiologically acceptable composition concomitantly or concurrently, with an insulin secretagogue or insulin sensitizing agent, for example, in the form of separate dosage units to be used simultaneously, separately or sequentially (e.g., before or after the secretagogue or before or after the sensitizing agent). Accordingly, provided is a pharmaceutical or physiologically acceptable composition and an insulin secretagogue or insulin sensitizing agent as a combined preparation for simultaneous, separate or sequential use for the improvement of glucose control in type II diabetes patients.
[0201] Thus, any test known in the art or a method described herein can be used to determine that a subject is insulin resistant, and an insulin resistant patient can then be treated according to the methods described herein to reduce or cure the insulin resistance. Alternatively, the methods described herein also can be used to prevent the development of insulin resistance in a subject, e.g., those known to have an increased risk of developing insulin-resistance.
[0202] As discussed, successful treatment of type II diabetes can be brought about by techniques that serve to agonize target molecule expression or function, or alternatively, antagonize target molecule expression or function. These techniques include administration of modulators that include, but are not limited to, small organic or inorganic molecules; antibodies (including, for example, polyclonal, monoclonal, humanized, anti-idiotypic, chimeric or single chain antibodies, and Fab, F(ab')2 and Fab expression library fragments, scFV molecules, and epitope-binding fragments thereof); and peptides, phosphopeptides, or polypeptides.
[0203] Further, antisense and ribozyme molecules that inhibit expression of the target gene can also be used to reduce the level of target gene expression, thus effectively reducing the level of target gene activity. Still further, triple helix molecules can be utilized in reducing the level of target gene activity. Antisense, ribozyme and triple helix molecules are discussed above. It is possible that the use of antisense, ribozyme, and/or triple helix molecules to reduce or inhibit mutant gene expression can also reduce or inhibit the transcription (triple helix) and/or translation (antisense, ribozyme) of mRNA produced by normal target gene alleles, such that the concentration of normal target gene product present can be lower than is necessary for a normal phenotype. In such cases, nucleic acid molecules that encode and express target gene polypeptides exhibiting normal target gene activity can be introduced into cells via gene therapy method. Alternatively, in instances in that the target gene encodes an extracellular polypeptide, it can be preferable to co¬ administer normal target gene polypeptide into the cell or tissue in order to maintain the requisite level of cellular or tissue target gene activity.
[0204] Another method by which nucleic acid molecules may be utilized in treating or preventing type II diabetes is use of aptamer molecules specific for target molecules. Aptamers are nucleic acid molecules having a tertiary structure which permits them to specifically bind to ligands (see, e.g., Osborne, et al, Curr. Opin. Chem. Biol.1(1): 5-9 (1997); and Patel, D. J., Curr. Opin. Chem. Biol. Jim; 1(1): 32-46 (1997)). [0205] Yet another method of utilizing nucleic acid molecules for type II diabetes treatment is gene therapy, which can also be referred to as allele therapy. Provided herein is a gene therapy method for treating type II diabetes in a subject, which comprises contacting one or more cells in the subject or from the subject with a nucleic acid having a first nucleotide sequence. Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with type II diabetes (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO: 1-20). The first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with type II diabetes than the second nucleotide sequence. The first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof. The subject is often a human. Allele therapy methods often are utilized in conjunction with a method of first determining whether a subject has genomic DNA that includes polymorphic variants associated with type II diabetes.
[0206] In another allele therapy embodiment, provided herein is a method which comprises contacting one or more cells in the subject or from the subject with a polypeptide encoded by a nucleic acid having a first nucleotide sequence. Genomic DNA in the subject comprises a second nucleotide sequence having one or more polymorphic variations associated with type II diabetes (e.g., the second nucleic acid has a nucleotide sequence in SEQ ID NO: 1-20). The first and second nucleotide sequences typically are substantially identical to one another, and the first nucleotide sequence comprises fewer polymorphic variations associated with type II diabetes than the second nucleotide sequence. The first nucleotide sequence may comprise a gene sequence that encodes a full-length polypeptide or a fragment thereof. The subject is often a human.
[0207] For antibody-based therapies, antibodies can be generated that are both specific for target molecules and that reduce target molecule activity. Such antibodies may be administered in instances where antagonizing a target molecule function is appropriate for the treatment of type II diabetes.
[0208] In circumstances where stimulating antibody production in an animal or a human subject by injection with a target molecule is harmful to the subject, it is possible to generate an immune response against the target molecule by use of anti-idiotypic antibodies (see, e.g., Herlyn, Ann. Med.; 31(1): 66-78 (1999); and Bhattacharya-Chatterjee & Foon, Cancer Treat. Res.; 94: 51- 68 (1998)). Introducing an anti-idiotypic antibody to a mammal or human subject often stimulates production of anti-anti-idiotypic antibodies, which typically are specific to the target molecule. Vaccines directed to type II diabetes also may be generated in this fashion.
[0209] In instances where the target molecule is intracellular and whole antibodies are used, internalizing antibodies may be preferred. Lipofectin or liposomes can be used to deliver the antibody or a fragment of the Fab region that binds to the target antigen into cells. Where fragments of the antibody are used, the smallest inhibitory fragment that binds to the target antigen is preferred. For example, peptides having an amino acid sequence corresponding to the Fv region of the antibody can be used. Alternatively, single chain neutralizing antibodies that bind to intracellular target antigens can also be administered. Such single chain antibodies can be administered, for example, by expressing nucleotide sequences encoding single-chain antibodies within the target cell population (see, e.g., Marasco et ah, Proc. Natl. Acad. ScL USA 90: 7889- 7893 (1993)).
[0210] Modulators can be administered to a patient at therapeutically effective doses to treat type π diabetes. A therapeutically effective dose refers to an amount of the modulator sufficient to result in amelioration of symptoms of type II diabetes. Toxicity and therapeutic efficacy of modulators can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED5O (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50ZED50. Modulators that exhibit large therapeutic indices are preferred. While modulators that exhibit toxic side effects can be used, care should be taken to design a delivery system that targets such molecules to the site of affected tissue in order to minimize potential damage to uninfected cells, thereby reducing side effects.
[0211] Data obtained from cell culture assays and animal studies can be used in formulating a range of dosages for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage can vary within this range depending upon the dosage form employed and the route of administration utilized. For any compound used in the methods described herein, the therapeutically effective dose can be estimated initially from cell culture assays. A dose can be foπnulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test compound that achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma can be measured, for example, by high performance liquid chromatography.
[0212] Another example of effective dose determination for an individual is the ability to directly assay levels of "free" and "bound" compound in the serum of the test subject. Such assays may utilize antibody mimics and/or "biosensors" that have been created through molecular imprinting techniques. Molecules that modulate target molecule activity are used as a template, or "imprinting molecule", to spatially organize polymerizable monomers prior to their polymerization with catalytic reagents. The subsequent removal of the imprinted molecule leaves a polymer matrix which contains a repeated "negative image" of the compound and is able to selectively rebind the molecule under biological assay conditions. A detailed review of this technique can be seen in Ansell et at, Current Opinion in Biotechnology 7: 89-94 (1996) and in Shea, Trends in Polymer Science 2: \tt-\Tb (1994). Such "imprinted" affinity matrixes are amenable to ligand-binding assays, whereby the immobilized monoclonal antibody component is replaced by an appropriately imprinted matrix. An example of the use of such matrixes in this way can be seen in Vlatakis, et at, Nature 361: 645-647 (1993). Through the use of isotope-labeling, the "free" concentration of compound which modulates target molecule expression or activity readily can be monitored and used in calculations Of IC50. Such "imprinted" affinity matrixes can also be designed to include fluorescent groups whose photon-emitting properties measurably change upon local and selective binding of target compound. These changes readily can be assayed in real time using appropriate fiberoptic devices, in turn allowing the dose in a test subject to be quickly optimized based on its individual IC50. An example of such a "biosensor" is discussed in Kriz et at, Analytical Chemistry 67: 2142-2144 (1995).
[0213] The examples set forth below are intended to illustrate but not limit the invention.
Examples
[0214] In the following studies a group of subjects were selected according to specific parameters relating to type II diabetes. Nucleic acid samples obtained from individuals in the study group were subjected to genetic analysis, which identified associations between type II diabetes and certain polymorphic variants in SLC22A1, TIN, LOCI 12609, TRPSl1 PRAME, ZNF221, FOXA2, JAMS and GLlS (herein referred to as "target genes", "target nucleotides", "target polypeptides" or simply "targets"). Methods are described for producing SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLlS polypeptides and polypeptide variants in vitro or in vivo. SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, F0XA2, JAMS and GLlS nucleic acids or polypeptides and variants thereof are utilized for screening test molecules for those that interact with SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLIS molecules. Test molecules identified as interactors with SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GLIS molecules and variants are further screened in vivo to determine whether they treat breast cancer. Example 1
Samples and Pooling Strategies Sample Selection
[0215] Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to as case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age-matched controls. A database was created that listed all phenotypic trait information gathered from individuals for each case and control sample. Genomic DNA was extracted from each of the blood samples for genetic analyses.
DNA Extraction from Blood Samples
[0216] Six to ten milliliters of whole blood was transferred to a 50 ml tube containing 27 ml of red cell lysis solution (RCL). The tube was inverted until the contents were mixed. Each tube was incubated for 10 minutes at room temperature and inverted once during the incubation. The tubes were then centrifuged for 20 minutes at 3000 x g and the supernatant was carefully poured off. 100-200 μl of residual liquid was left in the tube and was pipetted repeatedly to resuspend the pellet in the residual supernatant. White cell lysis solution (WCL) was added to the tube and pipetted repeatedly until completely mixed. While no incubation was normally required, the solution was incubated at 37°C or room temperature if cell clumps were visible after mixing until the solution was homogeneous. 2 ml of protein precipitation was added to the cell lysate. The mixtures were vortexed vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate, and then centrifuged for 10 minutes at 3000 x g. The supernatant containing the DNA was then poured into a clean 15 ml tube, which contained 7 ml of 100% isopropanol. The samples were mixed by inverting the tubes gently until white threads of DNA were visible. Samples were centrifuged for 3 minutes at 2000 x g and the DNA was visible as a small white pellet. The supernatant was decanted and 5 ml of 70% ethanol was added to each tube. Each tube was inverted several times to wash the DNA pellet, and then centrifuged for 1 minute at 2000 x g. The ethanol was decanted and each tube was drained on clean absorbent paper. The DNA was dried in the tube by inversion for 10 minutes, and then 1000 μl of IX TE was added. The size of each sample was estimated, and less TE buffer was added during the following DNA hydration step if the sample was smaller. The DNA was allowed to rehydrate overnight at room temperature, and DNA samples were stored at 2-80C.
[0217] DNA was quantified by placing samples on a hematology mixer for at least 1 hour. DNA was serially diluted (typically 1:80, 1:160, 1:320, and 1:640 dilutions) so that it would be within the measurable range of standards. 125 μl of diluted DNA was transferred to a clear U- bottom microtitre plate, and 125 μl of IX TE buffer was transferred into each well using a multichannel pipette. The DNA and IX TE were mixed by repeated pipetting at least 15 times, and then the plates were sealed. 50 μl of diluted DNA was added to wells A5-H12 of a black flat bottom microtitre plate. Standards were inverted six times to mix them, and then 50 μl of IX TE buffer was pipetted into well Al, 1000 ng/ml of standard was pipetted into well A2, 500 ng/ml of standard was pipetted into well A3, and 250 ng/ml of standard was pipetted into well A4. PicoGreen (Molecular Probes, Eugene, Oregon) was thawed and freshly diluted 1 :200 according to the number of plates that were being measured. PicoGreen was vortexed and then 50μl was pipetted into all wells of the black plate with the diluted DNA. DNA and PicoGreen were mixed by pipetting repeatedly at least 10 times with the multichannel pipette. The plate was placed into a Fluoroskan Ascent Machine (microplate fluorometer produced by Labsystems) and the samples were allowed to incubate for 3 minutes before the machine was run using filter pairs 485 nm excitation and 538 nm emission wavelengths. Samples having measured DNA concentrations of greater than 450 ng/μl were re-measured for conformation. Samples having measured DNA concentrations of 20 ng/μl or less were re-measured for confirmation.
Pooling Strategies
[0218] Samples were placed into one of four groups based on disease status. The four groups were female case samples, female control samples, male case samples and male control samples. A select set of samples from each group were utilized to generate pools, and one pool was created for each group. Each individual sample in a pool was represented by an equal amount of genomic DNA. For example, where 25 ng of genomic DNA was utilized in each PCR reaction and there were 200 individuals in each pool, each individual would provide 125 pg of genomic DNA. Inclusion or exclusion of samples for a pool was based upon the following criteria and detailed in the tables below: patient ethnicity, diagnosis with type II diabetes, GAD antibody concentration, HbAIc concentration, body mass (BMI), patient age, date of primary diagnosis, and age of individual as of primary diagnosis. (See Table 1 below). Cases with elevated GAD antibody titers and low age of diagnosis were excluded to increase the homogeneity of the diabetes sample in terms of underlying pathogenesis. Controls with elevated HbAIc were excluded to remove any potentially undiagnosed diabetics. Control samples were derived from non-diabetic individuals with no family history of type II diabetes. Secondary phenotypes were also measured in the diabetic cases, including HDL levels, LDL levels, triglyceride levels, insulin levels, C-peptide levels, nephropathy status, and neuropathy status, to name a few. The phenotype data collected may be used to perform secondary analysis of the cases in order to elucidate the potential pathway of a disease gene. TABLE l
Figure imgf000075_0001
[0219] The selection process yielded the pools described in Table 2, which were used in the studies described herein.
TABLE 2
Figure imgf000075_0002
Example 2
Association of Polymorphic Variants with Type II Diabetes
[0220] A whole-genome screen was performed to identify particular SNPs associated with occurrence of type II diabetes. As described in Example 1, two sets of samples were utilized: female individuals having type II diabetes (female cases) and samples from female individuals not having type II diabetes or any history of type II diabetes (female controls), and male individuals having type II diabetes (male cases) and samples from male individuals not having type II diabetes or any history of type II diabetes (male controls). The initial screen of each pool was performed in an allelotyping study, in which certain samples in each group were pooled. By pooling DNA from each group, an allele frequency for each SNP in each group was calculated. These allele frequencies were then compared to one another. Particular SNPs were considered as being associated with type II diabetes when allele frequency differences calculated between case and control pools were statistically significant. SNP disease association results obtained from the allelotyping study were then validated by genotyping each associated SNP across all samples from each pool. The results of the genotyping were then analyzed, allele frequencies for each group were calculated from the individual genotyping results, and a p-value was calculated to determine whether the case and control groups had statistically significantly differences in allele frequencies for a particular SNP. When the genotyping results agreed with the original allelotyping results, the SNP disease association was considered validated at the genetic level.
SNP Panel Used for Genetic Analyses
[0221] A whole-genome SNP screen began with an initial screen of approximately 25,000 SNPs over each set of disease and control samples using a pooling approach. The pools studied in the screen are described in Example 1. The SNPs analyzed in this study were part of a set of 25,488 SNPs confirmed as being statistically polymorphic as each is characterized as having a minor allele frequency of greater than 10%. The SNPs in the set reside in genes or in close proximity to genes, and many reside in gene exons. Specifically, SNPs in the set are located in exons, introns, and within 5,000 base-pairs upstream of a transcription start site of a gene. In addition, SNPs were selected according to the following criteria: they are located in ESTs; they are located in Locuslink or Ensembl genes; and they are located in Genomatix promoter predictions. SNPs in the set were also selected on the basis of even spacing across the genome, as depicted in Table 3. An additional 3088 SNPs were included with these 25,488 SNPs and these additional SNPs had been chosen on the basis of gene location, with preference to non-synonymous coding SNPs located in disease candidate genes.
TABLE 3
Figure imgf000076_0001
Allelotyping and Genotyping Results
[0222] The genetic studies summarized above and described in more detail below identified allelic variants associated with type II diabetes. The allelic variants identified from the SNP panel described in Table 3 are summarized below in Table 4. TABLE 4
Figure imgf000077_0001
[0223] Table 4 includes information pertaining to the incident polymorphic variant associated with type II diabetes identified herein. Public information pertaining to the polymorphism and the genomic sequence that includes the polymorphism are indicated. The genomic sequences identified in Table 4 may be accessed at the http address www.ncbi.nih.gov/entrez/query.fcgi, for example, by using the publicly available SNP reference number (e.g., rs662138). The chromosome position refers to the position of the SNP within NCBFs Genome Build 34, which may be accessed at the following http address: www.ncbi.nlm.nih.gov/mapview/map_search.cgi?chr=hum_chr.inf&query=. The "Contig Position" provided in Table 4 corresponds to a nucleotide position set forth in the contig sequence, and designates the polymorphic site corresponding to the SNP reference number. The sequence containing the polymorphisms also may be referenced by the "Sequence Identification" set forth in Table 4. The "Sequence Identification" corresponds to cDNA sequence that encodes associated target polypeptides (e.g., SLC22A1) of the invention. The position of the SNP within the cDNA sequence is provided in the "Sequence Position" column of Table 4. Also, the allelic variation at the polymorphic site and the allelic variant identified as associated with type II diabetes is specified in Table 4. All nucleotide sequences referenced and accessed by the parameters set forth in Table 4 are incorporated herein by reference.
Assay for Verifying, Allelotvping, and Genotyping SNPs
[0224] A MassARRAY® system (Sequenom, Inc.) was utilized to perform SNP genotyping in a high-throughput fashion. This genotyping platform was complemented by a homogeneous, single-tube assay method (hME™ or homogeneous MassEXTEND™ (Sequenom, Inc.)) in which two genotyping primers anneal to and amplify a genomic target surrounding a polymorphic site of interest. A third primer (the MassEXTEND™ primer), which is complementary to the amplified target up to but not including the polymorphism, was then enzymatically extended one or a few bases through the polymorphic site and then terminated.
[0225] For each polymorphism, SpectroDESIGNER™ software (Sequenom, Inc.) was used to generate a set of PCR primers and a MassEXTEND™ primer was used to genotype the polymorphism. Table 5 shows PCR primers and Table 6 shows extension primers used for analyzing polymorphisms. The initial PCR amplification reaction was performed in a 5 μl total volume containing IX PCR buffer with 1.5 mM MgCl2 (Qiagen), 200 μM each of dATP, dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units of HotStar DNA polymerase (Qiagen), and 200 nM each of forward and reverse PCR primers specific for the polymorphic region of interest. TABLE 5: PCR Primers
Figure imgf000079_0001
[0226] Samples were incubated at 95°C for 15 minutes, followed by 45 cycles of 95°C for 20 seconds, 56°C for 30 seconds, and 720C for 1 minute, finishing with a 3 minute final extension at 72°C. Following amplification, shrimp alkaline phosphatase (SAP) (0.3 units in a 2 μl volume) (Amersham Pharmacia) was added to each reaction (total reaction volume was 7 μl) to remove any residual dNTPs. that were not consumed in the PCR step. Samples were incubated for 20 minutes at 37°C, followed by 5 minutes at 85°C to denature the SAP.
[0227] Once the SAP reaction was complete, a primer extension reaction was initiated by adding a polymorphism-specific MassEXTEND™ primer cocktail to each sample. Each MassEXTEND™ cocktail included a specific combination of dideoxynucleotides (ddNTPs}and deoxynucleotides (dNTPs) used to distinguish polymorphic alleles from one another. In Table 6, ddNTPs are shown and the fourth nucleotide not shown is the dNTP.
TABLE 6: Extend Primers
Figure imgf000079_0002
[0228] The MassEXTEND™ reaction was performed in a total volume of 9 μl, with the addition of IX ThermoSequenase buffer, 0.576 units of ThermoSequenase (Amersham Pharmacia), 600 nM MassEXTEND™ primer, 2 mM of ddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTP or dGTP or dTTP. The deoxy nucleotide (dNTP) used in the assay normally was complementary to the nucleotide at the polymorphic site in the amplicon. Samples were incubated at 94°C for 2 minutes, followed by 55 cycles of 5 seconds at 94°C, 5 seconds at 52°C, and 5 seconds at 72°C.
[0229] Following incubation, samples were desalted by adding 16 μl of water (total reaction volume was 25 μl), 3 mg of SpectroCLEAN™ sample cleaning beads (Sequenom, Inc.) and allowed to incubate for 3 minutes with rotation. Samples were then robotically dispensed using a piezoelectric dispensing device (SpectroJET™ (Sequenom, Inc.)) onto either 96-spot or 384-spot silicon chips containing a matrix that crystallized each sample (SpectroCFflP® (Sequenom, Inc.)). Subsequently, MALDI-TOF mass spectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (Bruker Daltonics) can be used) and SpectroTYPER RT™ software (Sequenom, Inc.) were used to analyze and interpret the SNP genotype for each sample.
Genetic Analysis
[0230} Variations identified in the target genes are provided in their respective genomic sequences (see SEQ ID Nos:l-20) Minor allelic frequencies for these polymorphisms was verified as being 10% or greater by determining the allelic frequencies using the extension assay described above in a group of samples isolated from 92 individuals originating from the state of Utah in the United States, Venezuela and France (Coriell cell repositories). • -
[0231] Genotyping results for the allelic variant set forth in Table 4 are shown for female pools in Table 7, for male pools in Table 8, and the combined results in Table 9. In Table 7, "F case" and "F control" refer to female case and female control groups, and in Table 8, "M case" and "M control" refer to male case and male control groups. In Tables 7, 8 and 9, "AF" refers to allele frequency.
Figure imgf000080_0001
Figure imgf000081_0001
Figure imgf000082_0001
[0232] The single marker alleles set forth in Tables 7, 8 and 9 were considered validated, since the genotyping data for the females, males, or both pools were significantly associated with type II diabetes, and because the genotyping results agreed with the original allelotyping results. Particularly significant associations with type II diabetes are indicated by a calculated p-value of less than 0.05 for genotype results, which are set forth in bold text.
[0233] SNP rs366111 is a coding non-synonymous SNP (G/A), which results in an amino acid change from arginine to glycine. The G allele is. associated with an increased risk of type II diabetes and codes for glycine; therefore, individuals with the glycine residue also have an increased risk of type II diabetes.
[0234] Odds ratio results are shown in Tables 7, 8 and 9. An odds ratio is an unbiased estimate of relative risk which can be obtained from most case-control studies. Relative risk (RR) is an estimate of the likelihood of disease in the exposed group (susceptibility allele or genotype carriers) compared to the unexposed group (not carriers). It can be calculated by the following equation:
RR = /A/7a
/A is the incidence of disease in the A carriers and /a is the incidence of disease in the non- carriers.
RR > 1 indicates the A allele increases disease susceptibility.
RR < 1 indicates the a allele increases disease susceptibility.
For example, RR = 1.5 indicates that carriers of the A allele have 1.5 times the risk of disease than non-carriers, i.e., 50% more likely to get the disease.
[0235] Case-control studies do not allow the direct estimation of /A and /a, therefore relative risk cannot be directly estimated. However, the odds ratio (OR) can be calculated using the following equation: OR = (nDAnda)/(ndAnDa) =pDA(l - pdA)/pdA(l - pDA), or OR = ((case f) / (1- case f)) / ((control f) / (1-control f)), where f = susceptibility allele frequency.
[0236] An odds ratio can be interpreted in the same way a relative risk is interpreted and can be directly estimated using the data from case-control studies, i.e., case and control allele frequencies. The higher the odds ratio value, the larger the effect that particular allele has on the development of type II diabetes. Possessing an allele associated with a relatively high odds ratio translates to having a higher risk of developing or having type II diabetes.
Example 3
Samples and Pooling Strategies for the Replication Cohort
[0237] The single marker polymorphisms set forth in Table 4 were genotyped again in two replication cohorts to further validate their association with type II diabetes. Like the original study population described in Examples 1 and 2, the replication cohorts consisted of type II diabetics (cases.) and non-diabetics (controls). The case and control samples were selected and genotyped as described below.
Sample Selection and Pooling Strategies - Newfoundland
[0238] Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to as case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age-matched controls. All of the samples were collected from individuals residing in Newfoundland, Canada. Residents of Newfoundland represent a preferred population for genetic studies because of their relatively small founder population and resulting homogeneity.
[0239] Genetic linkage studies from Newfoundland have proved particularly useful for mapping disease genes for both monogenic and complex diseases as evidenced in studies of autosomal dominant polycystic kidney disease, von Hippel-Lindau disease, ankylosing spondylitis, major depression, Grave's eye disease, retinitis pigmentosa, hereditary nonopolyposis colorectal cancer, Kallman syndrome, ocular albinism type I, late infantile type 2 neuronal ceroid lipofuscinosis, Bardet-Biedl syndrome, adenine phosphoriboysl-transferase deficiency, and arthropathy-camptodactyly syndrome, Familial multiple endocrine neoplasia type 1 (MENl). Thus Newfoundland's genetically enriched population provides a unique setting to rapidly identify disease-related genes in selected complex diseases.
[0240] Phenotypic trait information was gathered from individuals for each case and control sample, and genomic DNA was extracted from each of the blood samples for genetic analyses. [0241] Samples were placed into one of four groups based on disease status. The four groups were female case samples, female control samples, male case samples, and male control samples. A select set of samples from each group were utilized to generate pools, and one pool was created for each group.
[0242] Patients were included in the case pools if a) they were diagnosed with type II diabetes as documented in their medical record, b) they were treated with either insulin or oral hypoglycaemic agents, and c) they were of Caucasian ethnicity. Patients were excluded in the case pools if a) they were diabetic or had a history of diabetes, b) they suffered from diet controlled glucose intolerance, or c) they (or any their relatives) were diagnosed with MODY or gestational diabetes.
[0243] Phenotype information included, among others, patient ethnicity, country or origin of mother and father, diagnosis with type II diabetes (date of primary diagnosis, age of individual as of primary diagnosis), body weight, onset of obesity, retinopathy, glaucoma, cataracts, nephropathy, heart disease, hypertension, myocardial infarction, ulcers, required treatment (onset of insulin treatment, oral hypoglycemic agent), blood glucose levels, and MODY.
[0244] In total, the final selection consisted of 199 female cases, 241 Female Controls, 140 Male Case, and 62 Male Controls as set forth in Table 10.
TABLE 10
Figure imgf000084_0001
Sample Selection and Pooling Strategies - Denmark
[0245] The polymorphisms described in Table 4 were genotyped again in a second replication cohort, consisting of individuals of Danish ancestry, to further validate their association with type II diabetes. Blood samples were collected from individuals diagnosed with type II diabetes, which were referred to case samples. Also, blood samples were collected from individuals not diagnosed with type II diabetes or a history of type II diabetes; these samples served as gender and age- matched controls.
[0246] Phenotypic trait information was gathered from individuals for each case and control sample, and genomic DNA was extracted from each of the blood samples for genetic analyses. [0247] Samples were placed into one of four groups based on disease status. The four groups were female case samples, female control samples, male case samples, and male control samples. A select set of samples from each group were utilized to generate pools, and one pool was created for each group.
[0248] In total, the final selection consisted of 197 female cases (average age 63) and 277 male cases (average age 60) as set forth in Table 11. All cases had been diagnosed with type II diabetes in their mid 505S, and were of Danish ancestry. Members selected for the cohort were recruited through the outpatient clinic at Steno Diabetes Center, Copenhagen. Diabetes was diagnosed according to the 1985 World Health Organization criteria. For the controls, 152 females (average age 50), and 136 males (average age 55) were selected. All control subjects underwent a 2-hour oral glucose tolerance test (OGTT) and were deemed to be glucose tolerant, and all were of Danish ancestry. In addition, all control subjects were living in the same area of Copenhagen as the type II diabetic patients.
[0249] Additional phenotype were measured in both the case and control group. Phenotype information included, among others, e.g. body mass index , waist/hip ratio, blood pressure, serum insulin, glucose, C-peptide, cholesterol, hdl, triglyceride, HbAic, urine, creatinine, free fatty acids (mmol/1), GAD antibodies.
TABLE 11
Figure imgf000085_0001
DNA Extraction from Blood Samples
[0250] Blood samples for DNA preparation were taken in 5 EDTA tubes. If it was not possible to get a blood sample from a patient, a sample from the cheek mucosa was taken. Red blood cells were lysed to facilitate their separation from the white blood cells. The white cells were pelletted and lysed to release the DNA. Lysis was done in the presence of a DNA preservative using an anionic detergent to solubilize the cellular components. Contaminating RNA was removed by treatment with an RNA digesting enzyme. Cytoplasmic and nuclear proteins were removed by salt precipitation. [0251] Genomic DNA was then isolated by precipitation with alcohol (2-propanol and then ethanol) and rehydrated in water. The DNA was transferred to 2-ml tubes and stored at 40C for short-term storage and at -7O0C for long-term storage.
Example 4
Association of Polymorphic Variants with Type II Diabetes in the Replication Cohorts [0252] The associated SNP from the initial scan was re-validated by genotyping the associated SNP across the replication cohorts described in Example 3. The results of the genotyping were then analyzed, allele frequencies for each group were calculated from the individual genotyping results, and p-values were calculated to determine whether the case and control groups had statistically significant differences in allele frequencies for a particular SNP. The replication genotyping results with a calculated p-value of less than 0.05 were considered particularly significant, which are set forth in bold text. See Tables 12-14 herein.
Assay for Verifying, Allelotyping, and Genotvping SNPs
[0253] Genotyping of the replication cohort was performed using the same methods used for the original genotyping, as described herein. A MassARRAY® system (Sequenom, Inc.) was utilized to perform SNP genotyping in a high-throughput fashion. This genotyping platform was complemented by a homogeneous, single-tube assay method (hME™ or homogeneous MassEXTEND™ (Sequenom, Inc.)) in which two genotyping primers anneal to and amplify a genomic target surrounding a polymorphic site of interest. A third primer (the MassEXTEND™ primer), which is complementary to the amplified target up to but not including the polymorphism, was then enzymatically extended one or a few bases through the polymorphic site and then terminated.
[0254] For each polymorphism, SpectroDESIGNER™ software (Sequenom, Inc.) was used to generate a set of PCR primers and a MassEXTEND™ primer which where used to genotype the polymorphism. Other primer design software could be used or one of ordinary skill in the art could manually design primers based on his or her knowledge of the relevant factors and considerations in designing such primers. Table 6 shows PCR primers and Table 7 shows extension probes used for analyzing (e.g., genotyping) polymorphisms in the replication cohorts. The initial PCR amplification reaction was performed in a 5 μl total volume containing IX PCR buffer with 1.5 mM MgCl2 (Qiagen), 200 μM each of dATP, dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units of HotStar DNA polymerase (Qiagen), and 200 nM each of forward and reverse PCR primers specific for the polymorphic region of interest. [0255] Samples were incubated at 95°C for 15 minutes, followed by 45 cycles of 950C for 20 seconds, 560C for 30 seconds, and 72°C for 1 minute, finishing with a 3 minute final extension at 72°C. Following amplification, shrimp alkaline phosphatase (SAP) (0.3 units in a 2 μl volume) (Amersham Pharmacia) was added to each reaction (total reaction volume was 7 μl) to remove any residual dNTPs that were not consumed in the PCR step. Samples were incubated for 20 minutes at 37°C, followed by 5 minutes at 85°C to denature the SAP.
[0256] Once the SAP reaction was complete, a primer extension reaction was initiated by adding a polymorphism-specific MassEXTEND™ primer cocktail to. each sample. Each MassEXTEND™ cocktail included a specific combination of dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs) used to distinguish polymorphic alleles from one another. Methods for verifying, allelotyping and genotyping SNPs are disclosed, for example, in U.S. Pat. No. 6,258,538, the content of which is hereby incorporated by reference. In Table 7, ddNTPs are shown and the fourth nucleotide not shown is the dNTP.
[0257] The MassEXTEND™ reaction was performed in a total volume of 9 μl, with the addition of IX ThermoSequenase buffer, θ.576 units of ThermoSequenase (Amersham Pharmacia), 600 nM MassEXTEND™ primer, 2 mM of ddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTP or dGTP or dTTP. The deoxy nucleotide (dNTP) used in the assay normally was complementary to the nucleotide at the polymorphic site in the amplicon. Samples were incubated at 94°C for 2 minutes, followed by 55 cycles of 5 seconds at 94°C, 5 seconds at 52°C, and 5 seconds at 72°C.
[0258] Following incubation, samples were desalted by adding 16 μl of water (total reaction volume was 25 μl), 3 mg of SpectroCLEAN™ sample cleaning beads (Sequenom, Inc.) and allowed to incubate for 3 minutes with rotation. Samples were then robotically dispensed using a piezoelectric dispensing device (SpectroJET™ (Sequenom, Inc.)) onto either 96-spot or 384-s.pot silicon chips containing a matrix that crystallized each sample (SpectroCHIP® (Sequenom, Inc.)). Subsequently, MALDI-TOF mass spectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (Bruker Daltonics) can be used) and SpectroTYPER RT™ software (Sequenom, Inc.) were used to analyze and interpret the SNP genotype for each sample.
Genetic Analysis
[0259] The minor allelic frequencies for the polymorphisms set forth in Table 4 were verified as being 10% or greater using the extension assay described above in a group of samples isolated from 92 individuals originating from the state of Utah in the United States, Venezuela and France (Coriell cell repositories). [0260] Replication genotyping results in both cohorts are shown for female pools in Table 12 and for male pools in Table 13.
TABLE 12: Female Replication Genotyping Results
Figure imgf000088_0001
TABLE 13: Male Replication Genotyping Results
Figure imgf000089_0001
[0261] Meta-analysis was performed on rs662138, rs3219, rslO24373, rs366111, rsl203877, rs470936 and rs846312 based on genotype results provided in Tables 7, 8, 9, 12 and 13. Figures 10A-G depict the combined meta analysis odds ratio for rs662138, rs3219, rslO24373, rs366111, rsl203877, rs470936 and rs846312 across the discovery samples and replication samples (see Examples 1-4). In Figures 10A-G, "TBN" refers to the discovery cohort, "NFL" refers to the Newfoundland replication cohort, and "Steno" refers to the Denmark replication cohort. The boxes are centered over the odds ratio for each sample, with the size of the box correlated to the contribution of each sample to the combined meta analysis odds ratio. The lines extending from each box are the 95% confidence interval values. The diamond is centered over the combined meta analysis odds ratio with the ends of the diamond depicting the 95% confidence interval values. The meta-analysis further illustrates the strong association each of the incident SNPs has with type II diabetes across multiple case and control samples.
[0262] The absence of a statistically significant association in the replication cohort for males should not be interpreted as minimizing the value of the original finding. There are many reasons why a biologically derived association identified in a sample from one population would not replicate in a sample from another population. The most important reason is differences in population history. Due to bottlenecks and founder effects, there may be common disease predisposing alleles present in one population that are relatively rare in another, leading to a lack of association in the candidate region. Also, because common diseases such as diabetes- are the result of susceptibilities in many genes, and many environmental risk factors, differences in population- specific genetic and environmental backgrounds could mask the effects of a biologically relevant allele. For these and other reasons, statistically strong results in the original, discovery sample that did not replicate in the Newfoundland replication sample or the Denmark replication sample may be further evaluated in additional replication cohorts and experimental systems.
Example 5
SLC22A1 Region Proximal SNPs
[0263] The SNP rs662138 associated with type II diabetes, described in Table 4 above, lies within intron 7 of SLC22A1. Ninety additional allelic variants proximal to rs662138 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 14. The chromosome position provided in column four of Table 14 is based on Genome "Build 34" of NCBFs GenBank. The "genome letter" corresponds to the particular allele that appears in NCBFs build 34 genomic sequence of the region (chromosome 6: positions 160423601-160523200), and the "deduced iupac" corresponds to the single letter IUPAC code for the SLC22A1 polymorphic variants as they appear in SEQ ID NO: 1. Also, the "genome letter" may differ from the alleles (A1/A2) provided in Table 14 (and in subsequent Tables that provide the same information) if the genome letter is on one strand and the alleles are on the complementary strand, thus having different strand orientations {i.e., reverse vs forward). TABLE 14
Figure imgf000091_0001
Figure imgf000092_0001
Assay for Verifying and AUelotyping SNPs
[0264] The methods used to verify and allelotype the twenty-five proximal SNPs of Table 14 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 15 and Table 16, respectively.
TABLE 15
Figure imgf000093_0001
Figure imgf000094_0001
Figure imgf000095_0001
TABLE 16
Figure imgf000095_0002
Figure imgf000096_0001
Figure imgf000097_0001
Genetic Analysis
[0265] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 17, 18 and 19 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rsl 888727 has the following case and control allele frequencies: case Al (C) = 0.427; case A2 (T) = 0.573; control Al (C) = 0.386; and control A2 (T) = 0.614, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 17
Figure imgf000097_0002
Figure imgf000098_0001
Figure imgf000099_0001
Figure imgf000100_0001
Figure imgf000101_0001
TABLE 19
Figure imgf000101_0002
Figure imgf000102_0001
Figure imgf000103_0001
[0266] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures IA- 1C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 1A-1C can be determined by consulting Tables 17-19. For example, the left-most X on the left graph is at position 160423829. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined. [0267] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models, in S eds J.M. Chambers, and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was, applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10'8 were truncated at that value.
[0268] Finally, the exons. and introns of the genes, in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0269] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 20, 21 and 22 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 20
Figure imgf000104_0001
Figure imgf000105_0001
Figure imgf000106_0001
TABLE 21
Figure imgf000107_0001
Figure imgf000108_0001
TABLE 22
Figure imgf000109_0001
Figure imgf000110_0001
Metformin Link
[0270] Wang et ah, 2002 highlighted an involvement of SLC22A 1 (also known as Organic Cation Transporter 1; OCTl) in hepatic and intestinal distribution of metformin {Pharmacology and Experimental Therapeutics 302: 510-515). Metformin, a biguanide, is widely used as an oral hypoglycemic agent for the treatment of type 2 diabetes mellitus. Metformin seems to ameliorate hyperglycemia by improving peripheral sensitivity to insulin, reducing gastrointestinal glucose absorption and hepatic glucose production (Caspary and Creutzfeldt, Diabetologia 7: 379-385, 1971; Ηunάal et aL, 2000 Diabetes 49: 2063-2069; Borst and Snellen, 2001 Life Sd 69: 1497- 1507), although the exact mechanism for its pharmacological action is not yet fully understood.
[0271} Using the SLC22A1 knockout mice, Wang et ah, clearly demonstrated the involvement of Octl in hepatic and intestinal distribution of metformin. Based on this link, using the SNP rs662138, the distribution of genotypes amongst the Steno (Denmark) diabetics who were on metformin and those on other anti-diabetic drugs was assessed. Approximately 456 male and female diabetics were genotyped for this polymorphism: 382 of these individuals were not on metformin and 74 of these diabetics were being treated with metformin. This data set included both males and females.
TABLE 23
Figure imgf000111_0001
[0272] In Table 23, Metformin + any other diabetic drug = Group 1; and any other combination of diabetic drug = Group 2.
[0273] Table 23 shows that the major allele C has been selected for through evolution, maybe in times of lack of substantial food, as happened with the Pima Indians. The results show that having the major allele C individuals have a greater risk of developing diabetes. In addition, they are less likely to be treated with metformin. Accordingly, it is believed that patients were treated and did not respond. Also, it is believed that the polymorphism in the SLC22A1 gene results, in an alteration in the metformin uptake and or signaling cascade thereby preventing the substrate of SLC22A1 binding. Usually when metformin is administered to diabetics it results in a decrease in hepatic glucose production (HGP). This decrease in HGP may not be seen in the individual with the polymorphism in SLC22A1. Thus administering the drug to these individuals would result in no overall improvement in glucose homeostasis. Example 6 TTN Proximal SNPs.
[0274] The SNP rs 1001238 is associated with type II diabetes in the examples above. One hundred thirty-four additional allelic variants proximal to rs 1001238 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 24. The chromosome position provided in column four of Table 24 is based on Genome "Build 34" of NCBPs GenBank.
TABLE 24
Figure imgf000112_0001
Figure imgf000113_0001
Figure imgf000114_0001
Figure imgf000115_0001
Assay for Verifying and Allelotvping SNPs
[0275] The methods used to verify and allelotype the eighty-five proximal SNPs of Table 24 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 25 and Table 26, respectively.
TABLE 25
Figure imgf000115_0002
Figure imgf000116_0001
Figure imgf000117_0001
Figure imgf000118_0001
TABLE 26
Figure imgf000118_0002
Figure imgf000119_0001
Figure imgf000120_0001
Figure imgf000121_0001
Genetic Analysis
[0276] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 27, 28 and 29 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs2218324 has the following case and control allele frequencies: case Al (C) = 0.042; case A2 (T) = 0.958; control Al (C) = 0.025; and control A2 (T) = 0.975, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 27: Female Allelotyping Results
Figure imgf000121_0002
Figure imgf000122_0001
Figure imgf000123_0001
Figure imgf000124_0001
Figure imgf000125_0001
Figure imgf000126_0001
Figure imgf000127_0001
Figure imgf000128_0001
Figure imgf000129_0001
Figure imgf000130_0001
[0277] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 2A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 2A-C can be determined by consulting Tables 27-29. For example, the left-most X on the left graph is at position 179567944. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0278] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10"8 were truncated at that value.
[0279] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0280] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples.3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 30, 31 and 32 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 30: Female Replication Allelotyping Results
Figure imgf000131_0001
Figure imgf000132_0001
Figure imgf000133_0001
Figure imgf000134_0001
Figure imgf000135_0001
Figure imgf000136_0001
Figure imgf000137_0001
TABLE 32: Combined Replication Allelotyping Results
Figure imgf000137_0002
Figure imgf000138_0001
Figure imgf000139_0001
Figure imgf000140_0001
Example 7
LOCI 12609 Proximal SNPs
[0281] The SNP rs3219 associated with type II diabetes in the examples above falls in the untranslated region of the LOCI 12609 gene. Fifty-one additional allelic variants proximal to rs3219 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 33. The chromosome position provided in column four of Table 33 is based on Genome "Build 34" of NCBPs GenBank.
TABLE 33
Figure imgf000140_0002
Figure imgf000141_0001
Figure imgf000142_0001
Figure imgf000143_0001
Assay for Verifying and Allelotyping SNPs
[0282} The methods used to verify and allelotype the seventy-one proximal SNPs of Table 33 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 34 and Table 35, respectively.
TABLE 34
Figure imgf000143_0002
Figure imgf000144_0001
Figure imgf000145_0001
TABLE 35
Figure imgf000145_0002
Figure imgf000146_0001
Figure imgf000147_0001
Figure imgf000148_0001
Genetic Analysis
[0283] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 36, 37 and 38 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs986469 has the following case and control allele frequencies: case Al (C) = 0.585; case A2 (T) = 0.615; control Al (C) = 0.421; and control A2 (T) = 0.579, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 36
Figure imgf000148_0002
Figure imgf000149_0001
Figure imgf000150_0001
Figure imgf000151_0001
Figure imgf000152_0001
Figure imgf000153_0001
Figure imgf000154_0001
Figure imgf000155_0001
[0284] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 3A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 3A-C can be determined by consulting Tables 36-38. For example, the left-most X on the left graph is at position 84746396. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0285] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances, between SNPs. Two other lines are drawn to expose linear trends, in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10"8 were truncated at that value.
[0286] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is. place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0287] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping, results are shown for female (F), male (M), and combined cases and controls in Table 39, 40 and 41 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays. TABLE 39: Female Replication Allelotyping Results
Figure imgf000157_0001
Figure imgf000158_0001
Figure imgf000158_0002
Figure imgf000159_0001
Figure imgf000160_0001
Figure imgf000161_0001
Example 8
TRPSl Proximal SNPs
[0288] The SNP rsl 160333 associated with type II diabetes in the examples above falls within an intron of the trichorhinophalangeal syndrome I (TRPSl) gene. Sixty additional allelic variants proximal to rsl 160333 were identified and subsequently allelotyped in diabetes, case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 42. The chromosome position provided in column four of Table 42 is based on Genome "Build 34" of NCBFs GenBank.
TABLE 42
Figure imgf000161_0002
Figure imgf000162_0001
Figure imgf000163_0001
Assay for Verifying and Allelotyping SNPs
[0289] The methods used to verify and allelotype the sixty proximal SNPs of Table 42 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 43 and Table 44, respectively.
TABLE 43
Figure imgf000163_0002
Figure imgf000164_0001
Figure imgf000165_0001
TABLE 44
Figure imgf000166_0001
Figure imgf000167_0001
Figure imgf000168_0001
Genetic Analysis
[0290] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 45, 46 and 47 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs2625681 has the following case and control allele frequencies: case Al (T) = 0.002; case A2 (C) = 0.998; control Al (T) = 0.001 ; and control A2 (C) = 0.999, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 45
Figure imgf000168_0002
Figure imgf000169_0001
Figure imgf000170_0001
Figure imgf000171_0001
Figure imgf000172_0001
Figure imgf000173_0001
Figure imgf000174_0001
Figure imgf000175_0001
[0291] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 4A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives, the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 4A-C can be determined by consulting Tables 45-47. For example, the left-most X on the left graph is at position 116488511. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0292} To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10"8 were truncated at that value.
[0293] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription. Proximal SNP Replication
[0294] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 48, 49 and 50 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 48: Female Replication Allelotyping Results
Figure imgf000176_0001
Figure imgf000177_0001
Figure imgf000178_0001
Figure imgf000179_0001
Figure imgf000180_0001
Figure imgf000181_0001
Figure imgf000182_0001
Example 9
PRAME Proximal SNPs
[0295] The SNP rs 1024373 associated with type II diabetes in the examples above falls within an intron of the PRAME gene. One hundred thirty-two additional allelic variants proximal to rslO24373 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 51. The chromosome position provided in column four of Table 51 is based on Genome "Build 34" of NCBFs GenBank. The amino acid changes provided in column 8 of Table 51 correspond to SNPs that code for non-synonymous amino acid changes, where changes with two * occur in the SUHW2 polypeptide (locus ID no. 140883), changes with one * occur in the SUHWl polypeptide (locus ID no. 129025), and the W7R change occurs in the PRAME polypeptide.
TABLE 51
Figure imgf000183_0001
Figure imgf000184_0001
Figure imgf000185_0001
Figure imgf000186_0001
Assay for Verifying and Allelotyping SNPs
[0296] The methods used to verify and allelotype the 132 proximal SNPs of Table 51 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 52 and Table 53, respectively.
TABLE 52
Figure imgf000186_0002
Figure imgf000187_0001
Figure imgf000188_0001
Figure imgf000189_0001
TABLE 53
Figure imgf000189_0002
Figure imgf000190_0001
Figure imgf000191_0001
Figure imgf000192_0001
Genetic Analysis
[0297] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 54, 55 and 56 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rsl 1109 has the following case and control allele frequencies: case Al (C) = 0.913; case A2 (T) = 0.087; control Al (C>= 0.907; and control A2 (T) = 0.09-3, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 54
Figure imgf000192_0002
Figure imgf000193_0001
Figure imgf000194_0001
Figure imgf000195_0001
TABLE 55
Figure imgf000195_0002
Figure imgf000196_0001
Figure imgf000197_0001
Figure imgf000198_0001
Figure imgf000199_0001
Figure imgf000200_0001
Figure imgf000201_0001
[0298} Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 5A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures.5 A-C can be determined by consulting Tables 54-56. For example, the left-most X on the left graph is at position 21166760. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0299] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10"8 were truncated at that value.
[0300] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0301] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 57, 58 and 59 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 57: Female Replication Allelotyping Results
Figure imgf000202_0001
Figure imgf000203_0001
Figure imgf000204_0001
Figure imgf000205_0001
Figure imgf000206_0001
Figure imgf000207_0001
Figure imgf000208_0001
Figure imgf000209_0001
Figure imgf000210_0001
Figure imgf000211_0001
Example 10
ZNF221 Proximal SNPs
[0302] The SNP rs366111 associated with type II diabetes in the examples above falls within an exon of the ZNF221 gene. SNP r&366111 is a coding non-synonymous SNP (G/A), which results in an amino acid change from arginine to glycine. The G allele is associated with an increased risk of type II diabetes and codes for glycine; therefore, individuals with the glycine residue also have an increased risk of type II diabetes. One hundred forty-six additional allelic variants proximal to rs366111 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 60. The chromosome position provided in column four of Table 60 is based on Genome "Build 34" of NCBI's GenBank. The amino acid changes provided in column 8 of Table 60 correspond to SNPs that code for non-synonymous amino acid changes, where changes with one * occur in the ZNF221 polypeptide, changes with two * occur in the ZNFl 55 polypeptide (locus ID no. 7711), and changes with three * occur in the ZNF230 polypeptide (locus ID no. 7773).
TABLE 60
Figure imgf000211_0002
Figure imgf000212_0001
Figure imgf000213_0001
Figure imgf000214_0001
Assay for Verifying and Allelotyping SNPs
[0303] The methods used to verify and allelotype the eighty-one proximal SNPs of Table 60 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 61 and Table 62, respectively.
TABLE 61
Figure imgf000215_0001
Figure imgf000216_0001
Figure imgf000217_0001
Figure imgf000218_0001
TABLE 62
Figure imgf000218_0002
Figure imgf000219_0001
Figure imgf000220_0001
Figure imgf000221_0001
Genetic Analysis
[0304] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 63, 64 and 65 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs374652 has the following case and control allele frequencies: case Al (C) = 0.055; case A2 (G) = 0.945; control Al (C) = 0.040; and control A2 (G) = 0.960, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 63
Figure imgf000221_0002
Figure imgf000222_0001
Figure imgf000223_0001
Figure imgf000224_0001
TABLE 64
Figure imgf000225_0001
Figure imgf000226_0001
Figure imgf000227_0001
Figure imgf000228_0001
TABLE 65
Figure imgf000228_0002
Figure imgf000229_0001
Figure imgf000230_0001
Figure imgf000231_0001
[0305] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 6A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 6A-C can be determined by consulting Tables 63-65. For example, the left-most X on the left graph is at position 49113509. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0306] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10"8 were truncated at that value.
[0307] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0308] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 66, 61 and 68 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 66: Female Replication Allelotyping Results
Figure imgf000232_0001
Figure imgf000233_0001
Figure imgf000234_0001
Figure imgf000235_0001
Figure imgf000236_0001
Figure imgf000237_0001
Figure imgf000238_0001
Figure imgf000239_0001
Figure imgf000240_0001
Figure imgf000241_0001
Figure imgf000242_0001
Example 11
FOXA2 Proximal SNPs
[0309] The SNP rsl203877 associated with type II diabetes in the examples above falls near a member of the forkhead class of DNA-binding proteins called forkhead box A2 (FOXA2). One hundred additional allelic variants proximal to rs 1203877 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 69. The chromosome position provided in column four of Table 69 is based on Genome "Build 34" of NCBI's GenBank.
TABLE 69
Figure imgf000242_0002
Figure imgf000243_0001
Figure imgf000244_0001
Figure imgf000245_0001
Assay for Verifying and Allelotyping SNPs
[0310] The methods used to verify and allelotype the eighty-one proximal SNPs of Table 69 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 70 and Table 71 respectively.
TABLE 70
Figure imgf000245_0002
Figure imgf000246_0001
Figure imgf000247_0001
TABLE 71
DbSNP rs# Extend Primer Term Mix
1415817 GGGATGAAGGGGCTTCCC ACG
952991 GCAGTTTCTCTGCTTTGAA ACT
2404166 GCATTTTCACTTTTTAATATGGCT ACG
2896772 TTTGTGTGTCAGACTGTT ACG
2404167 AACAAACATCACTGGACTATAA ACG
1011104 GAGGTAAATTTACAGAGACAA ACT
1572580 CTCTGCTTGATAAGTGATTA ACT
1999861 CTCCTAGTCAGTTCCATCCT ACG
1999862 TTCCTACTGAGGGACTC ACG
1999863 GTGAGCTGAAGCTCTGA ACT
1572581 TTGTTGGTAAGAGTCAGTTTCT ACT
1890206 GGGTGACCTTCAGAGGTTGA ACT
1890207 CAGACTGAGGGCGTGTGTAC ACT
1890208 GTTCTGAAGACAGTGATG CGT
1415818 AGATGAAGAAGGTGGTC ACG
952690 TCAATCCATATCTCACTCTATAT CGT
1890210 GTCACTCTGTTACCCAA ACT
1933206 GAAAATCCTTGTGACTTAGACA ACT
2021680 I 1 1 I IATTTGTGCTCCTCC ACT
2021681 CGTGTGGCTATGGAGGA ACT
1203866 CAGGGAAAATTCCAGAC ACT
1203867 TTGGGAAGAAAAAAGGTTTAG ACG
1203868 CTTCAGAACTCAAAGGC CGT
1203869 CTGCTTAGGTTACAAGAC CGT
Figure imgf000248_0001
Figure imgf000249_0001
Genetic Analysis
[0311] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 72, 73 and 74 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rsl415817 has the following case and control allele frequencies: case Al (C) = 0.067; case A2 (T) = 0.933; control Al (C) = 0.074; and control A2 (T) = 0.926, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays. TABLE 72
Figure imgf000250_0001
Figure imgf000251_0001
Figure imgf000252_0001
Figure imgf000253_0001
Figure imgf000254_0001
TABLE 74
Figure imgf000254_0002
Figure imgf000255_0001
Figure imgf000256_0001
[0312] Allelotyping results were considered particularly significant with a calculated p- value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 7A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 7A-C can be determined by consulting Tables 12-1 A. For example, the left-most X on the left graph is at position 22487606. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0313] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than 10'8 were truncated at that value.
[0314] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Proximal SNP Replication
[0315] The proximal SNPs disclosed above were also allelotyped in the Newfoundland replication cohort described in Examples 3 and 4. Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 75, 76 and 77 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). Some SNPs may be labeled "untyped" because of failed assays.
TABLE 75: Female Replication Allelotyping Results
Figure imgf000257_0001
Figure imgf000258_0001
Figure imgf000259_0001
Figure imgf000260_0001
Figure imgf000261_0001
Figure imgf000262_0001
Figure imgf000263_0001
Figure imgf000264_0001
Additional Genotyping
[0316] A total of thirty-seven SNPs, including the incident SNP, were genotyped in the discovery and replication cohorts as described in Examples 1-4. Table 78 provides The PCR primers and extend primers used in these assays are provided in Table 79 and Table 80, respectively. TABLE 78
Figure imgf000265_0001
TABLE 79
Figure imgf000266_0001
TABLE 80
Figure imgf000266_0002
Figure imgf000267_0001
[0317] Tables 81-83, below, shows the case and control allele frequencies along with the p- values for all of the SNPs genotyped in female pools, male pools, and combined, respectively. P- values less than 0.05 are in bold.
Figure imgf000267_0002
Figure imgf000268_0001
Figure imgf000269_0001
Figure imgf000270_0001
Figure imgf000271_0001
Figure imgf000271_0002
Figure imgf000272_0001
Figure imgf000273_0001
Figure imgf000274_0001
Figure imgf000275_0001
Figure imgf000276_0001
Figure imgf000277_0001
Figure imgf000278_0001
Example 12 JAM3 Proximal SNPs
[0318] The SNP rs470936 associated with type II diabetes, in the examples above falls within an intron of the JAMS gene. One hundred ten additional allelic variants proximal to rs470936 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 84. The chromosome position provided in column four of Table 84 is based on Genome "Build 34" of NCBI's GenBank.
TABLE 84
Figure imgf000278_0002
Figure imgf000279_0001
Figure imgf000280_0001
Figure imgf000281_0001
Assay for Verifying and Allelotyping SNPs
[0319J The methods used to verify and allelotype the 110 proximal SNPs of Table 84 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 85 and Table 86, respectively.
TABLE 85
Figure imgf000281_0002
Figure imgf000282_0001
Figure imgf000283_0001
TABLE 86
Figure imgf000283_0002
Figure imgf000284_0001
Figure imgf000285_0001
Figure imgf000286_0001
Genetic Analysis
[0320] Allelotyping results are shown for female (F), male (M), and combined cases and controls in Table 87, 88 and 89 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs470855 has the following case and control allele frequencies: case Al (T) = 0.756; case A2 (C) = 0.244; control Al (T) = 0.784; and control A2 (C) = 0.216, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 87
Figure imgf000286_0002
Figure imgf000287_0001
Figure imgf000288_0001
Figure imgf000289_0001
Figure imgf000290_0001
Figure imgf000291_0001
Figure imgf000292_0001
Figure imgf000293_0001
[0321] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 8A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 8A-C can be determined by consulting Tables 87-89. For example, the left-most X on the left graph is at position 133498018. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0322] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two common significance levels,
Figure imgf000294_0001
and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs. to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and T.J. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p-values that were less than ICF8 were truncated at that value.
[0323} Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Example 13 GLI3 Proximal SNPs
[0324] The SNP rs846312 associated with type II diabetes in the examples above falls within • an intron of the GLI3 gene. Two hundred fifteen additional allelic variants proximal to rs846312 were identified and subsequently allelotyped in diabetes case and control sample sets as described in Examples 1 and 2. The polymorphic variants are set forth in Table 90. SNP rs846266 codes for a non-synonymous amino acid change (Tl 83A). The chromosome position provided in column four of Table 90 is based on Genome "Build 34" of NCBFs GenBank.
TABLE 90
Figure imgf000295_0001
Figure imgf000296_0001
Figure imgf000297_0001
Figure imgf000298_0001
Figure imgf000299_0001
Assay for Verifying and Allelotyping SNPs
[0325] The methods used to verity and allelotype the 215 proximal SNPs of Table 90 are the same methods described in Examples 1 and 2 herein. The primers and probes used in these assays are provided in Table 91 and Table 92, respectively.
TABLE 91
Figure imgf000299_0002
Figure imgf000300_0001
Figure imgf000301_0001
Figure imgf000302_0001
Figure imgf000303_0001
Figure imgf000304_0001
TABLE 92
Figure imgf000304_0002
Figure imgf000305_0001
Figure imgf000306_0001
Figure imgf000307_0001
Figure imgf000308_0001
Genetic Analysis
[0326] Allelotyping results are shown for female (F)5 male (M), and combined cases and controls in Table 93, 94 and 95 respectively. The allele frequency for the A2 allele is noted in the fifth and sixth columns for diabetes case pools and control pools, respectively, where "AF" is allele frequency. The allele frequency for the Al allele can be easily calculated by subtracting the A2 allele frequency from 1 (Al AF = 1-A2 AF). For example, the SNP rs846323 has the following case and control allele frequencies: case Al (T) = 0.554; case A2 (C) = 0.446; control Al (T) = 0.558; and control A2 (C) = 0.442, where the nucleotide is provided in parenthesis. Some SNPs are labeled "untyped" because of failed assays.
TABLE 93
Figure imgf000309_0001
Figure imgf000310_0001
Figure imgf000311_0001
Figure imgf000312_0001
Figure imgf000313_0001
TABLE 94
Figure imgf000314_0001
Figure imgf000315_0001
Figure imgf000316_0001
Figure imgf000317_0001
Figure imgf000318_0001
Figure imgf000319_0001
Figure imgf000320_0001
Figure imgf000321_0001
Figure imgf000322_0001
Figure imgf000323_0001
[0327] Allelotyping results were considered particularly significant with a calculated p-value of less than or equal to 0.05 for allelotype results. These values are indicated in bold. The allelotyping p-values were plotted in Figures 9A-C for females, males and combined, respectively. The position of each SNP on the chromosome is presented on the x-axis. The y-axis gives the negative logarithm (base 10) of the p-value comparing the estimated allele in the case group to that of the control group. The minor allele frequency of the control group for each SNP designated by an X or other symbol on the graphs in Figures 9A-C can be determined by consulting Tables 93-95. For example, the left-most X on the left graph is at position 41819727. By proceeding down the Table from top to bottom and across the graphs from left to right the allele frequency associated with each symbol shown can be determined.
[0328] To aid the interpretation, multiple lines have been added to the graph. The broken horizontal lines are drawn at two. common significance levels, 0.05 and 0.01. The vertical broken lines are drawn every 20kb to assist in the interpretation of distances between SNPs. Two other lines are drawn to expose linear trends in the association of SNPs to the disease. The light gray line (or generally bottom-most curve) is a nonlinear smoother through the data points on the graph using a local polynomial regression method (W.S. Cleveland, E. Grosse and W.M. Shyu (1992) Local regression models. Chapter 8 of Statistical Models in S eds J.M. Chambers and TJ. Hastie, Wadsworth & Brooks/Cole.). The black line provides a local test for excess statistical significance to identify regions of association. This was created by use of a 10kb sliding window with lkb step sizes. Within each window, a chi-square goodness of fit test was applied to compare the proportion of SNPs that were significant at a test wise level of 0.01, to the proportion that would be expected by chance alone (0.05 for the methods used here). Resulting p- values that were less than 10"8 were truncated at that value.
[0329] Finally, the exons and introns of the genes in the covered region are plotted below each graph at the appropriate chromosomal positions. The gene boundary is indicated by the broken horizontal line. The exon positions are shown as thick, unbroken bars. An arrow is place at the 3' end of each gene to show the direction of transcription.
Example 14 In Vitro Production of Target Polypeptides
[0330] cDNA is. cloned into a pIVEX 2.3-MCS vector (Roche Biochem} using a directional cloning method. A cDNA insert is prepared using PCR with forward and reverse primers having 5' restriction site tags, (in frame) and 5-6 additional nucleotides in addition to 3' gene-specific portions, the latter of which is typically about twenty to about twenty-five base pairs in length. A Sal I restriction site is introduced by the forward primer and a Sma I restriction site is introduced by the reverse primer. The ends of PCR products are cut with the corresponding restriction enzymes (i.e., Sal I and Sma I) and the products are gel-purified. The pIVEX 2.3-MCS vector is linearized using the same restriction enzymes, and the fragment with the correct sized fragment is isolated by gel-purification. Purified PCR product is ligated into the linearized pIVEX 2.3-MCS vector and E. coli cells transformed for plasmid amplification. The newly constructed expression vector is verified by restriction mapping and used for protein production.
[0331] E. coti lysate is reconstituted with 0.25 ml of Reconstitution Buffer, the Reaction Mix is reconstituted with 0.8 ml of Reconstitution Buffer; the Feeding Mix is reconstituted with 10.5 ml of Reconstitution Buffer; and the Energy Mix is reconstituted with 0.6 ml of Reconstitution Buffer. 0.5 ml of the Energy Mix was added to the Feeding Mix to obtain the Feeding Solution. 0.75 ml of Reaction Mix, 50 μl of Energy Mix, and 10 μg of the template DNA is added to the E. coli lysate.
[0332] Using the reaction device (Roche Biochem), 1 ml of the Reaction Solution is loaded into the reaction compartment. The reaction device is turned upside-down and 10 ml of the Feeding Solution is loaded into the feeding compartment. All lids are closed and the reaction device is loaded into the RTS500 instrument. The instrument is run at 300C for 24 hours with a stir bar speed of 150 rpm. The pIVEX 2.3 MCS vector includes a nucleotide sequence that encodes six consecutive histidine amino acids on the C-terminal end of the target polypeptide for the purpose of protein purification. Target polypeptide is purified by contacting the contents of reaction device with resin modified with Ni2+ ions. Target polypeptide is eluted from the resin with a solution containing free Ni2+ ions. Example H
Cellular Production of Target Polypeptides
[0333] Nucleic acids are cloned into DNA plasmids having phage recombination cites and target polypeptides are expressed therefrom in a variety of host cells. Alpha phage genomic DNA contains short sequences known as attP sites, and E. coli genomic DNA contains unique, short sequences known as attB sites. These regions share homology, allowing for integration of phage DNA into E. coli via directional, site-specific recombination using the phage protein Int and the E. coli protein IHF. Integration produces two new art sites, L and R, which flank the inserted prophage DNA. Phage excision from E. coli genomic DNA can also be accomplished using these two proteins with the addition of a second phage protein, Xis. DNA vectors have been produced where the integration/excision process is modified to allow for the directional integration or excision of a target DNA fragment into a backbone vector in a rapid in vitro reaction (Gateway™ Technology (Invitrogen, Inc.)).
[0334] A first step is to transfer the nucleic acid insert into a shuttle vector that contains attL sites surrounding the negative selection gene, ccdB (e.g. pENTER vector, Invitrogen, Inc.). This transfer process is accomplished by digesting the nucleic acid from a DNA vector used for sequencing, and to ligate it into the multicloning site of the shuttle vector, which will place it between the two attL sites while removing the negative selection gene ccdB. A second method is to amplify the nucleic acid by the polymerase chain reaction (PCR) with primers containing attB sites. The amplified fragment then is integrated into the shuttle vector using Int and IHF. A third method is to utilize a topoisomerase-mediated process, in which the nucleic acid is amplified via PCR using gene-specific primers with the 5' upstream primer containing an additional CACC sequence {e.g.,
TOPO® expression kit (Invitrogen, Inc.)). In conjunction with Topoisomerase I, the PCR amplified fragment can be cloned into the shuttle vector via the attL sites in the correct orientation.
[0335] Once the nucleic acid is transferred into the shuttle vector, it can be cloned into an expression vector having attR sites. Several vectors containing attR sites, for expression of target polypeptide as a native polypeptide, N-fusion polypeptide, and C-fusion polypeptides are commercially available (e.g. , pDEST (Invitrogen, Inc.)), and any vector can be converted into an expression vector for receiving a nucleic acid from the shuttle vector by introducing an insert having an attR site flanked by an antibiotic resistant gene for selection using the standard methods described above. Transfer of the nucleic acid from the shuttle vector is accomplished by directional recombination using Int, IHF, and Xis (LR clonase). Then the desired sequence can be transferred to an expression vector by carrying out a one hour incubation at room temperature with Int, IHF, and Xis, a ten minute incubation at 37°C with proteinase K, transforming bacteria and allowing expression for one hour, and then plating on selective media. Generally, 90% cloning efficiency is achieved by this method. Examples of expression vectors are pDEST 14 bacterial expression vector with att7 promoter, pDEST 15 bacterial expression vector with a T7 promoter and a N-terminal GST tag, pDEST 17 bacterial vector with a T7 promoter and a N-terminal polyhistidine affinity tag, and pDEST 12.2 mammalian expression vector with a CMV promoter and neo resistance gene. These expression vectors or others like them are transformed or transfected into cells for expression of the target polypeptide or polypeptide variants. These expression vectors are often transfected, for example, into murine-transformed a adipocyte cell line 3T3-L1, (ATCC), human embryonic kidney cell line 293, and rat cardiomyocyte cell line H9C2.
Example 16
In Vitro Tests of Metabolic-Related Activity
[0336] In vitro assays described hereafter are useful for identifying therapeutics for treating human diabetes. As used in Examples hereafter directed to in vitro assays, rodent models and studies in humans, the term "test molecule" refers to a molecule that is added to a system, where an agonist effect, antagonist effect, or lack of an effect of the molecule on SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 function or a related physiological function in the system is assessed. An example of a test molecule is a test compound, such as a test compound described in the section "Compositions Comprising Diabetes-Directed Molecules" above. Another example of a test molecule is a test peptide, which includes, for example, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3- related test peptide such as a soluble, extracellular form of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLB, a biologically active fragment of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3, a SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 binding partner or ligand, or a functional fragment of the foregoing. A concentration range or amount of test molecule utilized in the assays and models is selected from a variety of available ranges and amounts. For example, a test molecule sometimes is introduced to an assay system in a concentration range between 1 nanomolar and 100 micromolar or a concentration range between 1 nanograms/mL and 100 micrograms/mL. An effect of atest molecule on SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 function or a related physiological function often is determined by comparing an effect in a system administered the test molecule against an effect in system not admininstered the test molecule. Described directly hereafter are examples of in vitro assays. Glucose Uptake Assay
[0337] One of the many responses of adipocytes and muscle cells after exposure to insulin is the transport of glucose intracellularly. This transport is mediated by GLUT4, an insulin- regulatable glucose transporter. Insulin binding to insulin receptors on the cell surface results in autophosphorylation and activation of the intrinsic tyrosine kinase activity of the insulin receptor. Phosphorylated tyrosine residues on the insulin receptor and its endogenous targets activate several intracellular signaling pathways that eventually lead to the translocation of GLUT4 from intracellular stores to the extracellular membrane.
Methods
[0338] Cells are plated in 6-welI dishes, and grown to confluency. Cells are then differentiated with DMEM plus 10% fetal calf serum (FCS)5 10 ug/mL insulin, 390 ng/mL dexamethasone and 112 ug/mL isobutylmethylxanthine for 2 days. After 2 days of differentiation* media is changed to maintenance media DMEM plus. 10% FCS and 5 ug/mL insulin. Media is changed every 2 days thereafter. Cells are assayed for insulin-mediated glucose uptake 10 days after differentiation. On the day of the assay, cells are washed once with PBS, and serum starved by adding 2 mL of DMEM plus 2mg/mL BSA for 3 hours. During serum starvation, recombinant rat SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeric ligand is preclustered. In a solution of PBS plus 2 mg/niL BSA, recombinant rat SLC22A1, TTN3 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeria is added to a concentration of 1.75 ug/mL, and anti-human IgG, Fc γ fragment specific antibody to a final concentration of 17.5 ug/mL. After 3 hours of serum starvation, media is. replaced with 2 mL of preclustered SLC22A1, TTN5 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3, and incubated for 10, 40 and 90 min at 37 deg. After 10 min, porcine insulin is added to a final concentration of 100 nM for 10 min at 37 deg. For every 2 mL of media, 100 uL of PBS-2-DOG label is added to give a final concentration of 2 uCi. Cells are immediately placed on ice, washed three times with ice cold PBS, and lysed with 0.7 mL of 0.2 N NaOH. Lysates are read in a Wallac 1450 Microbeta Liquid Scintillation and Luminescence Counter.
Example 17
Triacylglycerol (TG) Assay
[0339] A direct metabolic consequence of glucose transport intracellularly is its incorporation into the fatty acid and glycerol moieties of triacylglycerol (TG). TGs are highly concentrated stores of metabolic energy, and are the major energy reservoir of cells. In mammals, the major site of accumulation of triacylglycerols is the cytoplasm of adipose cells. Adipocytes are specialized for the synthesis, and storage of TG, and for their mobilization into fuel molecules that are transported to other tissues through the bloodstream. It is likely that changes in the transport of glucose intracellularly can affect cytoplasmic stores of triacylglycerols.
Methods
[0340] Cells are plated in 6-well dishes, and grown to confluency. When cells reached confluency, cells are differentiated with DMEM plus 10% fetal calf serum (FCS), 10 ug/mL insulin, 390 ng/mL dexamethasone and 112 ug/mL isobutylmethylxanthine for 2 days. After 2 days of differentiation, media is changed to maintenance media DMEM plus 10% FCS and 5 ug/mL insulin. On the day of the assay (day 9 post-differentiation), cells are washed once with PBS, and serum starved by adding 2 mL of DMEM plus 2 mg/niL BSA for 3 hours. During serum starvation, recombinant rat SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeric ligand is preclustered. In a solution of PBS plus 2 mg/mL BSA recombinant rat SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3/Fc chimeria is added to a concentration of 1.75 ug/mL, and anti-human IgG, Fc γ fragment specific antibody to a final concentration of 17.5 ug/mL. After 3 hours of serum starvation, media is replaced with pre¬ clustered SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3 solution, and incubated for 10 minutes at 37 degrees. Cells are then treated with 100 nM porcine insulin for 2 hours at 37 degrees. Cells are immediately placed on ice, and washed twice with ice cold PBS. Cells are lysed with 1% SDS, 1.2 mM Tris, pH 7.0 and heat treated at 95 degrees for 5 minutes. Samples are assayed using INFINITY Tryglyceride reagent. In a 96-well, flat bottom, transparent microtiter plate, 3 uL of sample are added to 300 uL of INFINITY Triglyceride Reagent. Samples are incubated at room temperature for 10 minutes. The assay is read at 500-550 nm.
Example 18
Quantitative Assessment of mResistin Levels
[0341] Resistin is a secreted factor specifically expressed in white adipocyte. It was initially discovered in a screen for genes downregulated in adipocytes by PPAR gamma, and expression was found to be attenuated by insulin. Elevated levels of resistin have been measured in genetically obese, and high fat fed obese mice. It is therefore thought that resistin contributes to peripheral tissue insulin unresponsiveness, one of the pathological hallmarks of diabetes. Methods
[0342] 3T3-L1 cells are differentiated for 3 days as previously described and maintained for three days prior to splitting. At day 5 post-differentiation, differentiated cells are plated in 10 cm dish at a cell density of 3X106 cells. Cells are then serum starved on day 7 after initiation of differentiation. On day 8, cells are treated with pre-clustered recombinant rat SLC22A1, TTN5 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLBfFc chimera as described above for 10 min and treated with 10 nM insulin for 2 hours. Cells are harvested, mRNA extracted using magnetic DYNAL beads and reverse transcribed to cDNA using Superscript First-Strand Synthesis as described by the manufacturer. Appropriate primers are designed and used in 15 uL PCR reaction using 55 deg annealing temperature and 30 cycles of amplification.
Example 19 Effect on Muscle Differentiation
[0343] • C2C12 cells (murine skeletal muscle cell line; ATCC CRL 1772, Rockville, MD) are seeded sparsely (about 15-20%) in complete DMEM (w/glutamine, pen/strep, etc) + 10% FCS. Two days later they become 80-90% confluent. At this time, the media is changed to DMEM+2% horse serum to allow differentiation. The media is changed daily. Abundant myotube formation occurs after 3-4 days of being in 2% horse serum, although the exact time course of C2C12 differentiation depends on how long they have been passaged and how they have been maintained, among other factors.
[0344] To test the effect of the presence of test molecules on muscle differentiation, test molecules (e.g., test peptides added in a range of 1 to 2.5 μg/mL) are added the day after seeding when the cells are still in DMEM with 10% FCS. Two days after plating the cells (one day after the test molecule was first added), at about 80-90% confluency, the media is changed to DMEM+2% horse serum plus the test molecule.
Effect on Muscle Cell Fatty Acid Oxidation
[0345] C2C12 cells are differentiated in the presence or absence of 2 μg/mL test molecules for 4 days. On day 4, oleate oxidation rates are determined by measuring conversion of l-14C-oleate (0.2 mM) to 14CO2 for 90 min. This experiment can be used to screen for active polypeptides and peptides as well as agonists and antagonists or activators and inhibitors of SLC22Al, TTN1 LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLB polypeptides or binding partners.
[0346] The effect of test molecules on the rate of oleate oxidation can be compared in differentiated C2C12 cells (murine skeletal muscle cells; ATCC, Manassas, VA CRL-1772) and in a hepatocyte cell line (Hepal-6; ATCC, Manassas, VA CRL-1830). Cultured cells are maintained according to manufacturer's instructions. The oleate oxidation assay is performed as previously described (Muoio et al (1999) Biochem J 338;783-791). Briefly, nearly confluent myocytes are kept in low serum differentiation media (DMEM, 2.5% Horse serum) for 4 days, at which time formation of myotubes becomes maximal. Hepatocytes are kept in the same DMEM medium supplemented with 10% FCS for 2 days. One hour prior to the experiment the media is removed and 1 mL of preincubation media (MEM, 2.5% Horse serum, 3 mM glucose, 4 mM Glutamine, 25 mM Hepes, 1% FFA free BSA, 0.25 mM Oleate, 5 μg/mL gentamycin) is added. At the start of the oxidation experiment I4C-Oleic acid (lμCi/mL,. American Radiolabeled Chemical Inc., St. Louis, MO) is added and cells are incubated for 90 min at 37°C in the absence/presence of test molecule (e.g., 2.5 μg/mL of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GL/3-related test peptide). After the incubation period 0.75 mL of the media is removed and assayed for 14C-oxidation products as described below for the muscle FFA oxidation experiment.
Triglyceride and Protein Analysis, following Oleate Oxidation in Cultured Cells [0347] Following transfer of media for oleate oxidation assay, cells are placed on ice. To determine triglyceride and protein content, ceils are washed with 1 mL of Ix PBS to remove residual media. To each well 300 μL of cell dissociation solution (Sigma) is added and incubated at 370C for 10 min. Plates are tapped to loosen cells, and 0.5 mL of Ix PBS is added. The cell suspension is transferred to an Eppendorf tube, each well is rinsed with an additional 0.5 mL of Ix PBS, and is transferred to the appropriate Eppendorf tube. Samples are centrifuged at 1000 rpm for 10 minutes at room temperature. Each supernatant is discarded and 750 μL of Ix PBS/2% CHAPS is added to cell pellet. The cell suspension is vortexed and placed on ice for 1 hour. Samples are then centrifuged at 13000 rpm for 20 min at 40C. Each supernatant is transferred to a new tube and frozen at -2O0C until analyzed. Quantitative measure of triglyceride level in each sample is determined using Sigma Diagnostics GPO-TRINDER enzymatic kit. The procedure outlined in the manual is followed, with the following exceptions: the assay is performed in 48 well plate, 350 μL of sample volume is assayed, a control blank consists of 350 μL PBS/2% CHAPS, and a standard contains 10 μL standard provide in the kit with 690 μL PBS/2% CHAPS. Analysis of samples is carried out on a Packard Spectra Count at a wavelength of 550 nm. Protein analysis is carried out on 25 μL of each supernatant sample using the BCA protein assay (Pierce) following manufacturer's instructions. Analysis of samples is carried out on a Packard Spectra Count at a wavelength of 550 nm. Stimulation of insulin secretion in HIT-Tl 5 cells
[0348] HIT-T 15 (ATCC CRU1777) is an immortalized hamster insulin-producing cell line. It is known that stimulation of cAMP in HIT-T 15 cells causes an increase in insulin secretion when the glucose concentration in the culture media is changed from 3mM to 15 mM. Thus, test molecules also are tested for their ability to stimulate glucose-dependent insulin secretion (GSIS) in HIT-T15 cells. In this assay, 30,000 cells/well in a 12-well plate are incubated in culture media containing 3 mM glucose and no serum for 2 hours. The media is then changed, wells receive media containing either 3 mM or 15 mM glucose, and in both cases the media contains either vehicle (DMSO) or test molecule at a concentration of interest. Some wells receive media containing 1 micromolar forskolin as. a positive control. AU conditions are tested in triplicate. Cells are incubated for 30 minutes, and the amount of insulin secreted into the media is determined by ELISA, using a kit from either Peninsula Laboratories (Cat # ELIS-7536) or Crystal Chem Inc. (Cat # 90060).
Stimulation of insulin secretion in isolated rat islets
[0349] As with HIT-T 15 cells, it is known that stimulation of cAMP in isolated rat islets causes an increase in insulin secretion when the glucose concentration in the culture media is changed from 60 mg/dl to 300 mg/dl. Ligands are tested for their ability to stimulate GSIS in rat islet cultures. This assay is performed as follows:
1. Select 75-150 islet equivalents (IEQ) for each assay condition using a dissecting microscope. Incubate overnight in low-glucose culture medium. (Optional.)
2. Divide the islets evenly into triplicate samples of 25-40 islet equivalents per sample. Transfer to 40 μm mesh sterile cell strainers in wells of a 6- well plate with 5 ml of low (60 mg/dl) glucose Krebs-Ringers Buffer (KRB) assay medium.
3. Incubate 30 minutes (1 hour if overnight step skipped) at 37° C and 5% CO2. Save the supernatants if a positive control for the RIA is desired.
4. Move strainers with islets to new wells with 5ml/well low glucose KRB. This is the second pre-incubation and serves to remove residual or carryover insulin from the culture medium. Incubate 30 minutes.
5. Move strainers to next wells (Low 1) with 4 or 5 ml low glucose KRB. Incubate at 37° C for 30 minutes. Collect supernatants into low-binding polypropylene tubes pre- labelled for identification and keep cold.
6. Move strainers to high glucose wells (300mg/dl, which is equivalent to 16.7mM). Incubate and collect supernatants as before. Rinse islets in their strainers in low- glucose to remove residual insulin. If the rinse if to be collected for analysis, use one rinse well for each condition (i.e. set of triplicates.)
7. Move strainers to final wells with low-glucose assay medium (Low. T). Incubate and collect supernatants as before.
8. Maintaining a cold temperature, centrifuge supernatants at 1800rpm for 5 minutes at A- 8°C to remove small islets/islet pieces that escape the 40mm mesh. Remove all but lower 0.5 - 1 ml and distribute in duplicate to pre-labelled low-binding tubes. Freeze and store at <-20° C until insulin concentrations can be determined.
9. Insulin determinations are performed as above, or by Linco Labs as a custom service, using a rat insulin RIA (Cat. # RI-13K).
Example 20
Effect of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2. JAM3 and GLB- Related Test Peptides on Mice Fed a High-Fat Diet
[0350] Following is a representative rodent model for identifying thereapeutics for treating human diabetes. Experiments are performed using approximately 6 week old C57B1/6 mice (8 per group). All mice are housed individually. The mice are maintained on a high fat diet throughout each experiment. The high fat diet (cafeteria diet; D12331 from Research Diets, Inc.) has the following composition: protein kcal% 16, sucrose kcal% 26, and fat kcal% 58. The fat is primarily composed of coconut oil, hydrogenated.
[0351] After the mice are fed a high fat diet for 6 days, micro-osmotic pumps are inserted using isoflurane anesthesia, and are used to provide test molecule, saline, and a control molecule (e.g., an irrelevant peptide) to the mice subcutaneously (s.c.) for 18 days. For example, SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS an d GZ/5-related test peptides are provided at doses of 100, 50, 25, and 2.5 μg/day and an irrelevant peptide is provided at 10 μg/day. Body weight is measured on the first, third and fifth day of the high fat diet, and then daily after the start of treatment. Final blood samples are taken by cardiac puncture and are used to determine triglyceride (TG), total cholesterol (TC), glucose, leptin, and insulin levels. The amount of food consumed per day is also determined for each group.
Example 21
In vivo Effects of Test Molecules on Glucose Homeostasis in Mice
[0352] Following are representative rodent models for identifying thereapeutics for treating human diabetes. Oral Glucose tolerance test (oGTT)
[0353] Male C57bl/6N mice at age of 8 weeks are fasted for 18 hours and randomly grouped (n=l 1) to receive an SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLD -related test peptide, a test molecule at indicated doses, or with control extendin-4 (ex-4, 1 mg/kg), a GLP-I peptide analog known to stimulate glucose-dependent insulin secretion. Thirty minutes after administration of SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAMS and GZ/3-related test peptides, test compound and control ex-4, mice are administered orally with dextrose at 5 g/kg dose. Test molecule is delivered orally via a gavage needle (p.o. volume at 100 ml). Control Ex-4 is delivered intraperitoneally. Levels of blood glucose are determined at regular time points using Glucometer Elite XL (Bayer).
Acute response of db mice to test molecule
[0354] Male db mice (C57BL/KsOlahsd-Leprdb, diabetic, Harlan) at age of 10 weeks are randomly grouped (n=6) to receive vehicle (oral gavage), SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLD-related test peptides (at concentration of interest), test molecule (e.g., 60 mg/kg, or another concentration of interest, oral gavage), or Ex-4 (1 mg/kg, intraperitoneally). After peptide and/or compound administration, food is removed and blood glucose levels are determined at regular time intervals. Reduction in blood glucose at each time point may be expressed as percentage of original glucose levels, averaged from the number of animals for each group. Results show the effect SLC22A1, TTN, LOCI 12609, TRPSl, PRAME, ZNF221, FOXA2, JAM3 and GLI3-related test peptides and test molecules for improving glucose homeostasis in diabetic animals.
Example 22 Effect of Test Molecules, on Plasma Free Fatty Acid in C57 BL/6 Mice
[0355] Following is a representative rodent model for identifying thereapeutics for treating human diabetes. The effect of test molecules on postprandial lipemia (PPL) in normal C57BL6/J mice is tested.
[0356] The mice used in this experiment are fasted for 2 hours prior to the experiment after which a baseline blood sample is taken. All blood samples are taken from the tail using EDTA coated capillary tubes (50 μL each time point). At time 0 (8:30 AM), a standard high fat meal (6g butter, 6 g sunflower oil, 10 g nonfat dry milk, 1O g sucrose, 12 mL distilled water prepared fresh following Nb#6, JF, pg.l) is given by gavage (vol.=l% of body weight) to all animals.
[0357] Immediately following the high fat meal, a test molecule is injected i.p. in 100 μL saline (e.g., 25μg of test peptide). The same dose (25μg/mL in lOOμL) is again injected at 45 min and at 1 hr 45 min. Control animals are injected with saline (3xl00μL). Untreated and treated animals are handled in an alternating mode.
[0358] Blood samples are taken in hourly intervals, and are immediately put on ice. Plasma is prepared by centrifugation following each time point. Plasma is kept at -200C and free fatty acids (FFA), triglycerides (TG) and glucose are determined within 24 hours using standard test kits (Sigma and Wako). Due to the limited amount of plasma available, glucose is determined in duplicate using pooled samples. For each time point, equal volumes of plasma from all 8 animals per treatment group are pooled.
Example 23 Effect of Test Molecules on Plasma FFA, TG and Glucose in C57 BL/6 Mice
[0359] Following is a representative rodent model for identifying thereapeutics for treating human diabetes. The experimental procedure is similar to that described in Example 13. Briefly, 14 mice are fasted for 2 houra prior to the experiment after which a baseline blood sample is taken. All blood samples are taken from the tail using EDTA coated capillary tubes (50 μL each time point). At time 0 (9:00AM), a standard high fat meal (see Example 4) is given by gavage (vol.=l% of body weight) to all animals. Immediately following the high fat meal, 4 mice are injected with a test molecule i.p. in lOOμL saline (e.g., 25 μg of test peptide). The same dose is again injected at 45 min and at 1 hr 45 min. A second treatment group receives 3 times a higher amount of the test molecule (e.g., 50 μg of test peptide) at the same intervals. Control animals are injected with saline (e.g., 3xl00μL). Untreated and treated animals are handled in an alternating mode.
[0360] Blood samples are immediately put on ice. Plasma is prepared by centrifugation following each time point. Plasma is kept at -20 0C and free fatty acids (FFA), triglycerides (TG) and glucose are determined within 24 hours using standard test kits (Sigma and Wako).
Example 24
Effect of Test Molecules on FFA following Epinephrine Injection
[0361] Following is a representative rodent model for identifying thereapeutics for treating human diabetes. In mice, plasma free fatty acids increase after intragastric administration of a high fat/sucrose test meal. These free fatty acids are mostly produced by the activity of lipolytic enzymes i.e. lipoprotein lipase (LPL) and hepatic lipase (HL). In this species, these enzymes are found in significant amounts both bound to endothelium and freely circulating in plasma. Another source of plasma free fatty acids is hormone sensitive lipase (HSL) that releases free fatty acids from adipose tissue after β-adrenergic stimulation. To test whether test molecules also regulate the metabolism of free fatty acid released by HSL, mice are injected with epinephrine. [0362] Two groups of mice are given epinephrine (5μg) by intraperitoneal injection. A treated group is injected with a test molecule (e.g., 25 μg of test peptide) one hour before and again together with epinephrine, while control animals receive saline. Plasma is isolated and free fatty acids and glucose are measured as described above.
Example 25 Effect of Test Molecules on Muscle FFA Oxidation
[0363} Following is a representative rodent model for identifying thereapeutics for treating human diabetes. To investigate the effect of test molecules on muscle free fatty acid oxidation, intact hind limb muscles from C57BL/6J mice are isolated and FFA oxidation is measured using oleate as substrate (Clee, S. M. et al. Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis. J Lipid Res 41, 521-531 (2000); Muoio, D. M., Dohm, G. L., Tapscott, E. B. & Coleman, R. A. Leptin opposes insulin's effects on fatty acid partitioning in muscles isolated from obese ob/ob mice. Am J Physiol 276, E913-921 (1999)} Oleate oxidation in isolated muscle is measured as previously described (Cuendet et al (1976) J Clin Invest 58:1078-1088; Le Marchand- Brustel, Y., Jeanrenaud, B. & Freychet, P. Insulin binding and effects in isolated soleus muscle of lean and obese mice. Am J Physiol 234, E348-E358 (1978). Briefly, mice are sacrificed by cervical dislocation and soleus and EDL muscles are rapidly isolated from the hind limbs. The distal tendon of each muscle is tied to a piece of suture to facilitate transfer among different media. All incubations are carried out at 300C in 1.5 mL of Krebs-Henseleit bicarbonate buffer (118.6 mM NaCl, 4.76 mM KCl, 1.19 mM KH2PO4, 1.19 mM MgSO4, 2.54 mM CaCl2, 25mM NaHCO3, 10 mM Hepes, pH 7.4) supplemented with 4% FFA free bovine serum albumin (fraction V, PJA grade, Sigma) and 5 mM glucose (Sigma). The total concentration of oleate (Sigma) throughout the experiment is 0.25 mM. All media are oxygenated (95% O2; 5% CO2) prior to incubation. The gas mixture is hydrated throughout the experiment by bubbling through a gas washer (Kontes Inc., Vineland, NJ).
[0364] Muscles are rinsed for 30 min in incubation media with oxygenation. The muscles are then transferred to fresh media (1.5 mL) and incubated at 300C in the presence of lμCi/mL [1-14C] oleic acid (American Radiolabeled Chemicals). The incubation vials containing this media are sealed with a rubber septum from which a center well carrying a piece of Whatman paper (1.5 cm x 11.5 cm) is suspended.
[0365] After an initial incubation period of lOmin with constant oxygenation, gas circulation is removed to close the system to the outside environment and the muscles are incubated for 90 min at 300C. At the end of this period, 0.45 mL of Solvable (Packard Instruments, Meriden, CT) is injected onto the Whatman paper in the center well and oleate oxidation by the muscle is stopped by transferring the vial onto ice.
[0366] After 5 min, the muscle is removed from the medium, and an aliquot of 0.5 mL medium is also removed. The vials are closed again and 1 mL of 35% perchloric acid is injected with a syringe into the media by piercing through the rubber septum. The CO2 released from the acidified media is collected by a Solvable in the center well. After a 90 min collection period at 300C, the Whatman paper is removed from the center well and placed in scintillation vials containing 15 mL of scintillation fluid (HionicFlour, Packard Instruments, Meriden, CT). The amount of 14C radioactivity is quantitated by liquid scintillation counting. The rate of oleate oxidation is expressed as nmol oleate produced in 90min/g muscle.
[0367] To test the effect of test molecules on oleate oxidation, the each test molecule is added to the media (e.g., a final concentration of 2.5 μg/mL of test peptide) and maintained in the media throughout the procedure.
Example 26
Effect of Test Molecules on FFA following Intralipid Injection
[0368] Following is a representative rodent model for identifying thereapeutics for treating human diabetes. Two groups of mice are intravenously (tail vein) injected with 30 μL bolus of Intralipid-20% (Clintec) to generate a sudden rise in plasma FFAs, thus by-passing intestinal absorption. (Intralipid is an intravenous fat emulsion used in nutritional therapy). A treated group (treated with test molecule) is injected with a test molecule (e.g., 25 μg of a test peptide) at 30 and 60 minutes before Intralipid is given, while control animals receive saline. Plasma is isolated and FFAs are measured as described previously. The effect of a test molecule on the decay in plasma FFAs following the peak induced by Intralipid injection is then monitored.
Example 27
In Vivo Tests for Metabolic-related Activity in Rodent Diabetes Models [0369] Following are representative rodent models for identifying thereapeutics for treating human diabetes. As metabolic profiles differ among various animal models of obesity and diabetes, analysis of multiple models is undertaken to separate the effects of test molecules on hyperglycemia, hyperinsulinemia, hyperlipidemia and obesity. Mutations within colonies of laboratory animals and different sensitivities to dietary regimens have made the development of animal models with non-insulin dependent diabetes associated with obesity and insulin resistance possible. Genetic models such as db/db and ob/ob (See Diabetes, (1982) 31(1): 1-6) in mice and fa/fa in zucker rats have been developed by the various laboratories for understanding the pathophysiology of disease and testing the efficacy of new antidiabetic compounds (Diabetes, (1983) 32: 830-838; Annu Rep Sankyo Res Lab (1994) 46: 1-57). The homozygous animals, C57 BL/KsJ-db/db mice developed by Jackson Laboratory, US, are obese, hyperglycemic, hyperinsulinernic and insulin resistant (J Clin Invest, (1990) 85: 962-967), whereas heterozygous animals are lean and normoglycemic. The db/db mice progressively develop insulinopenia with age, a feature commonly observed in late stages of human type II diabetes when blood sugar levels are insufficiently controlled. The state of the pancreas and its course vary according to the models. Since this is a model of type II diabetes mellitus, test molecules are tested for blood sugar and triglycerides lowering activities. Zucker (fa/fa) rats are severely obese, hyperinsulinemic, and insulin resistant (Coleman, Diabetes 31 :1, 1982; E. Shafrir, in Diabetes Mellitus; H. Rifkin and D. Porte, Jr. Eds. (Elsevier Science Publishing Co., Inc., New York, ed. 4, 1990), pp. 299-340), and the fa/fa mutation may be the rat equivalent of the murine db mutation (Friedman et al., Cell 69:217- 220, 1992; Truett et al., Proc. Natl. Acad. Sci. USA 88:7806, 1991). Tubby (tub/tub>mice are characterized by obesity, moderate insulin resistance and hyperinsulinemia without significant hyperglycemia (Coleman et al., J. Heredity 81:424, 1990).
[0370] Previously, leptin is reported to reverse insulin resistance and diabetes mellitus in mice with congenital lipodystrophy (Shimomura et al. Nature 401 : 73-76 (1999). Leptin is found to be less effective in a different lipodystrophic rodent model of lipoatrophic diabetes (Gavrilova et al Nature 403: 850 (2000); hereby incorporated herein in its entirety including any drawings, figures, or tables).
[0371] The streptozotocin (STZ) model for chemically-induced diabetes is tested to examine the effects of hyperglycemia in the absence of obesity. STZ-treated animals are deficient in insulin and severely hyperglycemic (Coleman, Diabetes 31:1, 1982; E. Shafrir, in Diabetes Mellitus; H. Rifkin and D. Porte, Jr. Eds. (Elsevier Science Publishing Co., Inc., New York, ed. 4, 1990X pp. 299-340). The monosodium glutamate (MSG) model for chemically- induced obesity (Olney, Science 164:719, 1969; Cameron et al., Clin Exp Pharmacol Physiol 5:41, 1978), in which obesity is less severe than in the genetic models and develops without hyperphagia, hyperinsulinemia and insulin resistance, is also examined. Also, a non-chemical, non-genetic model for induction of obesity includes feeding rodents a high fat/high carbohydrate (cafeteria diet) diet ad libitum.
[0372] Test molecules are tested for reducing hyperglycemia in any or all of the above rodent diabetes models or in humans with type II diabetes or other metabolic diseases described previously or models based on other mammals. In some assays, the test molecule sometimes is combined with another compatible pharmacologically active antidiabetic agent such as insulin, leptin (US provisional application No 60/155,506), or troglitazone, either alone or in combination.
Tests described in Gavrilova et al. ((2000) Diabetes 49:1910-6; (2000) Nature 403:850) using A-ZIP/F-1 mice sometimes are utilized, test molecules are administered intraperitoneally, subcutaneously, intramuscularly or intravenously. Glucose and insulin levels of the mice are tested, food intake and liver weight monitored, and other factors, such as leptin, FFA, and TG levels, often are measured in these tests.
In Vivo Assay for Anti-hyperglvcemic Activity of Test Molecules
[0373] Genetically altered obese diabetic mice (db/db) (male, 7-9 weeks old) are housed (7-9 mice/cage) under standard laboratory conditions at 22° C and 50% relative humidity, and maintained on a diet of Purina rodent chow and water ad libitum. Prior to treatment, blood is collected from the tail vein of each animal and blood glucose concentrations are determined using One Touch Basic Glucose Monitor System (Lifescan). Mice that have plasma glucose levels between 250 to 500 mg/dl are used. Each treatment group consists, of seven mice that are distributed so that the mean glucose levels are equivalent in each group at the start of the study, db/db mice are dosed by micro-osmotic pumps, inserted using isofturane anesthesia, to provide test molecules, saline, and an irrelevant peptide to the mice subcutaneously (s.c). Blood is sampled from the tail vein hourly for 4 hours and at 24, 30 h post-dosing and analyzed for blood glucose concentrations. Food is withdrawn from 0-4 h post dosing and reintroduced thereafter. Individual body weights and mean food consumption (each cage) are also measured after 24 h. Significant differences between groups (comparing test molecule treated to saline-treated) are evaluated using a Student t-test.
Example 28
Tests of Metabolic-Related Activity in Humans
[0374] Tests of the efficacy of test molecules in humans are performed in accordance with a physician's recommendations and with established guidelines. The parameters tested in mice are also tested in humans {e.g. food intake, weight, TG, TC, glucose, insulin, leptin, FFA). It is expected that the physiological factors are modified over the short term. Changes in weight gain sometimes require a longer period of time. In addition, diet often is carefully monitored. Test molecules often are administered in daily doses (e.g., about 6 mg test peptide per 70 kg person or about 10 mg per day). Other doses are tested, for instance 1 mg or 5 mg per day up to 20 mg, 50 mg, or 100 mg per day. [0375] Following is a genomic nucleotide sequence for a SLC22A1 region. The genomic nucleotide sequence is set forth in SEQ ID NO: 1. The following nucleotide representations are used throughout: "A" or "a" is adenosine, adenine, or adenylic acid; "C" or "c" is cytidine, cytosine, or cytidylic acid; "G" or "g" is guanosine, guanine, or guanylic acid; "T" or "t" is thymidine, thymine, or thymidylic acid; and "I" or "i" is inosine, hypoxanthine, or inosinic acid. Exons are indicated in italicized lower case type, introns are depicted in normal text lower case type, and polymorphic sites are depicted in bold upper case type. SNPs are designated by the following convention: "R" represents A or G, "M" represents A or C; "W" represents A or T; "Y" represents C or T; "S" represents C or G; "K" represents G or T; "V" represents A, C or G; "H" represents A, C, or T; "D" represents A, G, or T; "B" represents. C, G, or T; and 'TST" represents A, G, C, or T.
>6:160423601-160523200
1 gtactgcatg gccagcccct gctccgtatc ctgtcattag ccaaggagca aggccagccc
61 tctttttggg caaggttaaa ttcttgcctt ccctcttgtt gccgttgctt ctgttcctgg
121 aggagctcct ggcacagtga tggggtctca ggcctccacc ttactgcacg atgaagagtt
181 tgaggagatc aagaaggaga ctggcttttc ccacagtcaa atcacacgYc tgtacagccg
241 gttcagcaac ctggacaaag gagagaacag gacgatttcc aggggattcc agaacttgcc
301 atcaacccag tgggggactg gatcatcaat gccttccttc cagagggaga ggaccaggta
361 aacttccgtg gattcctgca aactctggct catttccaaa ccattgagga taatgaaaag
421 agcaaagttg tgaatggacc tgaaccactc aacagccgaa gcaacaaact gcagtttgct
481 tttcgactat atgatttgga taaagataac aagatctctc gtgatgagct gttaagggtg
541 ctgtgcatga tggtccaaat aaatatctca gataagcagc tgggcagtat cgcagacagg
601 accattcagg aggctgatca gcatggggac agtgccatat ctttatcaca gactttgcta
661 aggttttgga gaaggtggat gtagaacaga aaatgagcat ctgatttctt cactaaagga
721 cagacagcaa actattcctt gcagtctagt atttaagtac tggaacttga cagtcctcct
781 gtccaccagc cctacctcca ccccatcatt ccccttctcc caaagtacta ctgctgttgc
841 ataacaaccc caaatatgtt ctgtcaacac aaacctgctt ttggtctata aacagggcgt
901 tacagaatgg tacacccagt ctatttcttc tcagtatcca ttcgccagtt cttcatttat
961 aaatatcgtc ttcctctgtt ctgctgctga atgccacaca tccatccagt ctgagaaagt
1021 aagataggga atcctgccga ggaacaagcc agcaaagctc tttcactgga tgtagactgc
1081 acctgctgcc ttccctctgg cgagtctgcc agcatgcttc tccatccttt ttatatgtcc
1141 tttgcttccc acttctgtgt acattcaaca tactgttcac gtagtcatgc agtctttctg
1201 ctttttgtga agcctcagaa tctcctctgt tctacttggc accccaagct atgcctggta
1261 ttgtatttct gacttggctc gatagttcgg tggtctggca gtttttatct accttcatta
1321 ttaaaaggcc ctctggagct gggcgtggtg gctcacgcct gtaatcccag cactgtggga
1381 ggctgaggtg ggcagatcac gaggtcagga gatcgagacc atcctggcta acacggtgaa
1441 accccgtctt tactaaaaat acaaaaaatt agccgggcgt ggtggcgggt gcctgtagtc
1501 ccagctactc gggaggctga ggcaggagaa tggcgtgaac ctgggaggca gagcttgcag
1561 tgagccaaga tcgtgccact acactccagc ctgggcgaca gagcgagact ccatctcaaa
1621 aaaaaaaaaa aaaaaaggcc ctctgggatg ttgcctctcc aggagctttt tggtaaccaa
1681 cacttctctc gggagtatga gatcatcctc tgcactcttc tctgtcatca aagggctgct
1741 gggtggagat attcttgaaa ggtggccttg gtgagaggtg tggagtcaag tcttttaggt
1801 tgcttgccca cgtcactcta cctctggcct ctgattctca actttgtacc tatggggcgt
1861 ctcttgttag tgcagtgttg actattcaaa aagtagcaat atgcctgcag atttgcttag
1921 tcttgggaca cggttaccac cagaacggct gctcagacaa tatgcttggg gactttctca
1981 tggctttttg aacaaggagg ctgcgcaccc tgtgaagcct cctgcattca cacctctgct
2041 gcatggttta tgcctcagtg ttatgtgcac tggaatgctt ttcactatac gtttccaagt
2101 tagaaagatt agtgttatgg aagtgcctaa tgtatcctag tccaaaatat ttaaagattg
2161 ttctctgtgt cttgaagttt tgcacaaaat tctttatgaa ttttacactt ggcaaatgtt
2221 aatgctagaa gccatagtcc gcttcttata caagtccaaa gcgttgactg ttgtatcatt
2281 agctccctgg ttacactggt ttcccagctc cttgtgtaga ccactgctaa tcccttagaa
2341 acaagaggtc tggcactagt agcacaacct aaggtggcat tccaagtctt taagcaagtc
2401 acagcaactt ttctgccaaa gtcagcttag tttagacttc agtgaatcag gctgttgcca
2461 tcctaatgta tgtctctgtg agtctgttca ttcacatatc tgccgttggc tgactttctt
2521 gactcgctgc ttgcttgctt gtttccttgc tttggaaagc tattgaagat gtgtacataa
2581 ttctttagga aggggattgc taaaaaatac actgcaaagc gatggaaaac agtggagaac 2641 aggggagtag ccaggctgga tggctcaaat ataaatgaat gaggaattct ttatgaagtg 2701 tcagttggac tttgtgattg agtgatgtaa tacaggaatt atatagaaag ggaagaatat 2761 ctgatactga tctgttagat acttcagagg ctccttgatt tgcatattta aagttcctaa 2821 aattgtagct tttcccttct tttggctgta cagcagactg ttttaatcca cggttgtgcc 2881 ttattgttcc attaaaattg tgtcttcagt ccatcaataa atactcgtgg ttaaaacaaa 2941 acaaaaattc tttactgcat gctgactaag ggcagggctc tatccatgtg catcgagtgg 3001 cctcgtaagc tgtgaacaga gtgcctgtgg gacgtgggat gaagtctctt ctgcctgatt 3061 ttaagtcttt gcaaactttt tagaccgtaa ggagctaagc tcagtctgct cgtgaaaaat 3121 gatggtgaca tgccatgtgt gcccttcccg tttgacagac ggcgatctcg atgtcgtgtt 3181 tgcctcttcc tctaagtgcg gaaaggataa gaccaagtct gtttcttcca ccatcttctt 3241 ccactgtgac cctctggtgg aggacgggat ccccgagttc agtcacgaga ctgccgactg 3301 ccagtacctc ttctcttggt acacctcagc cgtgtgtcct ctggggtgag tatgacatcc 3361 ggaagcttag cacttgtacc ccacatcttc ccttaagaat aagtgtatgt gtgttttttc 3421 cccaatgttt tcttgtgaaa tattcagaac atgcaccaaa aaatagagaa aatgatagaa 3481 gcggacccca catagtcagt gccccgctcc gttgcttgtc agcatcctgc cactgatttt 3541 tggtccatgg ctgcgtcacc tcccaccacc cccagYagca gtgccattgt ttcctttgtg 3601 tgtaccatgc cccgaatagg gtccagtcag ggcaccccag ctctgtccct ctaccctgtt 3661 cccattgctg cctccctctc cttagctcct gggaggcaag tactggtggt gcctcctctg 3721 tgtaatcctg gaacatcttt tgtgtcgtaa tacaaatgat tgtttggtaa agtttcactt 3781 tgatttgagt gcagtatttt tactttgatt tgagtgcagt atttttacgt ttgtttgaat 3841 gaatgaaaag cttactctcc agattttgag aatcagcatg agaacagagg gagcagtttc 3901 tgctctgaag gaaggtggca cctccattgg ttgtcttgtg tcccacagcc gttttatact 3961 ttgccacgaa taacatgttg tcagttgctt tcctcttgga tttattttct tcttatgtta 4021 cccccaccca tcattgtatt tatatttatt tttatttttt atttttttgg gatggactct 4081 tgctctgtca ctcaggctgg agtgcagtgg tgcggtcttg gctcgttgca acctctgcct 4141 cctggttcag acgattctcc tgcctcagcc tcccaagtag ctgggattgc aggcatgtgt 4201 cattatgccc tgctaatttt tgtatttttg gtagagatgg ggtttcacca tgttgaccag 4261 gctggtcttg aactcctcac ctcagataat ctgcctgcct tggcctccca aagtgctggc 4321 attacaagcg tgagctgcca tgcccggcct ggcccaccat tttacataag. cttttcattc 4381 tctgtcctga gactttaaga ttgggggaac atgaattctt gccatgtaat tgtttccttc 4441 atgtcctgaa aaagggaccg tagagtcagc agacgacagt ggtgggggtc atgggggaat 4501 gaagatcaca cattttacat cttaaatcag cactccccct gcccaccctt aacagtggac 4561 atgagctcag caaaaaccag ctttgtcctg tgctgttcag gcctgtgtac ttagggatcc 4621 tcagctgcac agtcagggaa agaggctttt ggttgaaatt tcacttgtgg tattttcagc 4681 ttttgaatgt ttatggtgtt ccggtgacgt cattacaaaa tgaaaggatt gaaactggaa 4741 gttcttcctg aaagaagcaa aatgtgaatt tttataaaag ataaaaccca gaagtgaact 4801 ttccctgcat tctctgtcat ggagaggcta cagctgccat gagatgaatc ctgcctcctg 4861 acatggcagc tggtcttctg tgtgggtttg agggaccagg ccaggctagg atcccgtcca 4921 tgttgaatgt ttagagttcg acttctgtga gtaggaaaag cacYgtttat agggctttgg 4981 tgtttgactc taaaaccaga gaggaagatt ctccaggaaa cctcggtttc atgatgttca 5041 gaacatcagg attgggagct gtttaaatcc cttaacacac tgacaaggta ataaggactt 5101 taaaaaaaag aaacagaagt tgaaaccaag agcaaaaact cagatggtgg attttgaggg 5161 atgcaggtac cacccggggg tgacgtggaa tagtgcctga gatttttttt tccccaaatg 5221 ttatcagtgt tgtacattgt agaatcatgt aaatatggcc agacgcggtg gctcacgctt 5281 gtaatcccaa cattttggga ggctaaggtg ggcggatcac ttgaggtcag gagttctaga 5341 ccagcctggg caacatggtg aaaccccgtc tctactaaaa atacaaaaat tagccaggtg 5401 tggtggtgca cacctgtaat cgcagctact cagaaggctg aggcaggaga attgcttgaa 5461 cctggaaggt ggaggttgca gtgagccgag atggcgtcac tgtactgcag cctgggtgac 5521 agagcgagac tagatctcag gaaaaaaaat catgtaacta taacaaacag gtagcaatgt 5581 tcgccctaat agattaccat ttttagttct tttagctatt tcttctggaa tttacctcag 5641 tattttaaat taacatccta tatctcatct gttgacttcc tgcaatagta gatgaagata 5701 gagctgtctt acaccttcct cctactttac aaaaatggtt ctttcacaat atttgattaa 5761 agtcaaaaaa tttacacaaa gctaaaagac aggcatctac agatggaagg ggcaggctat 5821 gtgcccagca cagttattga tgacaagacc cacccactta tgaaatacca ggagagcaga 5881 tacaaagaaa agactgtaaa agctttaggg gaggctaatt tgttgtatta gaaaataatt 5941 caagaaaggt taaaatactt taaatcctca ctttatatca tcgataggtt cttggaaact 6001 gcaactttat gtgaaatgat gtataatgaa accagttttt tttcctcagc tttatgttat 6061 gttatgttat gttatgttat gttatgttat gttatgttat ttttttgaga cagagtctcg 6121 ctctcgccca ggctggagtg cagtggcgcg atctcggctc actgcaagct ctgcctccca 6181 ggttcacgcc attctcctgc ctcagcctcc caagtaactg ggactacagg ggcccgctac 6241 catgcccggc taattttttt gtatttttag tagagacggg gtttcaccgt gttagccagg 6301 ttgatctcga tctcctgatc ttgtgatccg cctgcctcag cctcccaaag tgctgggatt 6361 acaggcgtga gccaccgtgc ccggcctcat caactttata atgagacagt tttgaaggaa 6421 atgatgtcat tcgaggacct cccatagtcg cttcacggaa ggttgcagtt tgaaggaaac 6481 tgtcagcgac cctatgagga cacactgtac tggacaacag tggtgtctaa gctgagaggg 6541 gtggcagagt tcccagcttt taaaatttct tacagtgata gcatcatatg aaagtaaaac 6601 cattttcttg aaagaaatga aggaaggaag aaaaggtaga gttcctaatt gtttggaaga
6661 catactgtta gacttcctgt cttagtatgc atgccagtat tttaagaata accactaaaa
6721 taatataaat atagtatgta acttccaaaa cagcggagaa gaaaaggaga agtaaaaata
6781 cttaaatccc caaagaagac aagcaaggag aaaaataaaa caaaaatcag ataagtagaa
6841 atttcagccc agtatggtag aaataaatct aaatatatct ataatcataa gtatagcttg
6901 actaaacttg tcagctaaaa aacaagttgt caaactggat ttcaaaaaca actctgttta
6961 cagggaacat gcctaaaaca aggatacaga aaagaccaaa gtcaaaggag aagaaaagat
7021 ataccagaaa ataatctgaa agaaagctgc agtagttatg ttaatataag ataaaatagg
7081 atattaaaga aaaatattag ggataaagaa taaagagggt cattataatt ttaaaagggc
7141 taatttccta ggaagataca agaatcctaa acttccatct acctaataaa aaagccttga
7201 tatgtaaagc agaaattgac aaaaccacac acaagaaaca agcctagtga ttgctgggtt
7261 gagtagacaa agagtaaagc tttttttgga agattgagac agcacagtga aaagcaagat
7321 ttaatagaca tgcgcagaac actgttccct gcgttgggag aacactgttt gcccaagctt
7381 acatcaaaca cttagaaaac tttagcaaaa attaaccaca aagcaggcaa gtcagcaatt
7441 tccctttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
7501 tttttttttt tttttttttt tttttttgga gacagagact tgctcttttg cccaggctgg
7561 agtgcagtgg tgcgatcttg gctcactgca acctctgctt cccagattca agtgattctc
7621 atgcctcagc ctcctgagta gctgggatta tgggcgtgtg ccactacctc ctggctaatt
7681 tttgtatttt tagtagagat ggggtttcac catgttggcc aggctggtct caaactcctg
7741 acctcaggtg atccacctgc ctcagcctcc tagagtgctg ggattacagg catgagccac
7801 tgtgctcggc ctaagtcagc agatttctga ctgatgcaat taactacata catgatgcaa
7861 ttaagttagg aaccaagaac aaacaactga aaaatagaaa tacgtttgaa gactaaaact
7921 cacttctgaa taatctatgg tccaaagaag aaatcaaagg tagaaagtaa, aatacgctta
7981 gaactgagtg atgatgtgtc tcttggatgc atttcgagtg gtcctgagtg gctggagctg
8041 cttctgggtg agtgggtttc tggct'tggag ggagaggtct gcatagctct cagacctgtc
8101 tgtggagtgt ggtggtgctt ctttctgagc agaggtcaac ccttgcaggc ccacctagaa
8161 ctggacgcca ggctgtccct gtggtttctt gtcttgctga tgttttagat tgtttggctt
8221 aagctgaact ttgagagcgg cagcccttgc ggctgggccg cgggttggcc tgttgccctc
8281 aacctcagtt ctttgagcag ccggcatctt cagcagcaca agatgcgccc cgctggggtt
8341 gcctcttatc accagcagca caggaggaat ttctgtttat gtgatcctgt tcctgtgtgt
8401 gtggccccaa gtgacagagg gccagagaga ttagaagtgt caagtcgtta cactcttgag
8461 tgtcatcttg ctccctaagc gcaaagggaa gtctccgtgg ttgtttctca ttttctatta
8521 ttgtcccaac tctgcttgag tgttcgatgt catggaaacg cactgctttt tcccacaagt
8581 gtgcatgtgg gtcttgggtg ggaatggtga actccgctgg tgaggcagct ggtgtgctca
8641 agagccttct cctgggatgg agcaggcttg gagagaggag acccgtcggg ggctgtgctg
8701 ggctctgctg ctccctgggc cccaccctgg tctgtggccg cccctgggcc ccgtcctggc
8761 ctgtttctaa ggccccctgg gcccctttcg tgtggagatg gtgtctcatg gtgggctgcg
8821 ctcactgctt ccagaagaaa ttctgagagg aaagagctSt gaccctggag agaagtgtgg
8881 tgcttcggga cagcctctgc cccagcctag tcctgccgtg gattgggcca cctagagggg
8941 gccgcgtccc tcctgcctgt ctcctctgtg tttgggtgaa tgatagttca tggagctgga
9001 agcagtgcct tcttgtctgc tttctgacaa ccctgccaca aactcatcat caggaacttt
9061 ttgcagccct cttccatgtg gagtgggact tgtggccttg ttttctgggc aggagtaaga
9121 atcacatggt gttgggaaag tcagagctgc tcttgccttg gggactcagg tctcaggttg
9181 tggctgtggc agcaggacca ccctgtgaca cggctccttt tctgtgacgt ccttgcaggg
9241 tgggctttga cagcgagaat cccggggacg acgggcagat gcacaagggg ctgtcagaac
9301 ggagccaggc agtcggcgcg gtgctcagcc tgctgctggt ggcgctcacc tgctgcctgc
9361 tggccctgtt gctctacaag aaggagagga ggtaagYggg tggcagggcg aggtggggcg
9421 ggtggatgca tgcctcccat agctaatctt ggggtcagtt ttgtggggtt ttatttattt
9481 gtttttaagc cctacagcag cgaaggctcg aggttcttag tccaaaactc tctggaagca
9541 gtccccagcg ttgtggtttt taaatgcctg catttctgtc tgaccaaggc cgaggccctc
9601 gcacatcacc cgtgcctgcg gcttctagga ccgtggtcct ttgtgtccaa agatgagtag
9661 aacattaaac ggcacctttc atgggtgcct tttcccactg agcagctgac ccctctgccc
9721 tcctcacatc ataaatgcag gagtttatta agcgcctgct atgtaccagg tatgatgcca
9781 tccttttgcg ccactttgct gggtgaaagg actctccatg tgacggtcaa gcagattttc
9841 acgtcaggcg tcagtgttag tggctctgac ctgggggcag gagctggcca gctcaggcgt
9901 tccctgctgc cactgtcatt tgccagctga gccacaggcc aatgagagag gtggtcagaa
9961 agtaccaacc atggaggttg cagtgagcca agatctcacc actgcactct agcctgggca
10021 acaSagtgag actctgtcca aaaaaaaaaa aaaagtaccg attgtggccc tctacaccca
10081 aaatgcagaa tggggcagaa gaaggtctgg gccgggaagg gcatgtgatg tctgtctctg
10141 cgagccacca ttgggcaggc ttagtggcga cggactggtg tggtcctgtt agtcatgtga
10201 aaaagtcctt gccctaatgt taagttgctc tagagggccc tctgctgttg gattccaaag
10261 cccatSttct ttccactcaa tcacaacaaa tacattaggg tatatataac ctgtcacggc
10321 tactcatttg tcattcagaa tgtgggagaa atgcagccRc caccagcccc ctggtggtgc
10381 agatgggggt ggggctggcg ggggtggggc tcctgcccat gccctctcta cactggagta
10441 attattgtct cctttttttt Watagggaaa cagtgataag taagctgacc acttgctgta
10501 ggagaagttc caacgtgtcc tacaaatact caaaggtaat tttctgtggc gagtctcttg 10561 aaggcctgcc tccccggccc cctgtgctgc gctgtccatg tcgttctcat caggggtgcc
10621 aaggaaagca gtcacaggct gactggaatc cagaaaggaa agcaacaccc gttgcctcag
10681 ccacttacgc cagcacattt ttttgttatt tttttccctc aagtttctct cgtggttaca
10741 actgatttcc ttgaagtatt taataattag gttgtttcca ctgtttttgg ctgttcttta
10801 aaaaaaaaac aaaacacaca cacacacagt agtgaagata gttgtgcatg tagcatttgg
10861 atgatttctt tcagRtaaat ttgcagaaaa gaggcattat ctggatcaaa ggacataatg
10921 tttgtggtct cgatgggtaa tgatgaattg ctctctaaaa ctaggctttt gactSgagtc
10981 cctcctttga cattcctccc ctgccatgca gtgggcacct ttcagtctat gagtagcccg
11041 gaagagcccc ttctccccca gctgcgctgg gggatcactg gtctgctttt cggtccaggc
11101 catcccgaac gccttctgct tggtcccacc cttggctgtg agactcctgc caaatgaccc
11161 cactgccacc ctcccaaacc ctcctgcctt ggtgcagtct cattaggaac aggaaggtgt
11221 tgccagccct cgtcttcctt tccctgggaa ctggagatgc agtgactgtg aggacagtgg
11281 gccagttctt ccccagctcg tgccagcaga gagcatccct gatgtggtgg atggtggagc
11341 agaatggggt ctcttagggg gctcacgtgg tctctgctgt tgatccctgg caggtgaata
11401 aggaagaaga gacagatgag aatgaaacag agtggctgat ggaagagatc cagctgcctc
11461 ctccacggca gggaaaggaa gggcaggaga acggccatat taccaccaag tcagtgaaag
11521 ccctcagctc cctgcatggg gatgaccagg acagtgagga tgaggttctg accatcccag
11581 aggtgaaagt tcactcgggc aggggagctg gggcagagag ctcccaccca gtgagaaacg
11641 cacagagcaa tgcccttcag gagcgtgagg acgatagggt ggggctggtc aggggtgaga
11701 aggcgaggaa agggaagtcc agctctgcac agcagaagac agtgagctcc accaagctgg
11761 tgtccttcca tgacgacagc gacgaggacc tcttacacat ctgactccgc agtgcctgca
11821 ggggagcacg gagccgcggg acagccaagc acctccaacc aaataagact tccactcgat
11881 gatgcttcta taattttgcc tttaacagaa actttcaaaa gggaagagtt tttgtgatgg
11941 gggagagggt gaaggaggtc aggccccact ccttcctgat tgtttacagt cattggaata
12001 aggcatggct cagatcggcc acagggcggt accttgtgcc cagggttttg ccccaagtcc
12061 tcatttaaaa gcataaggcc ggacgcatct caaaacagag ggctgcattc gaagaaaccc
12121 ttgctgcttt agtcccgata gggtatttga ccccgatata ttttagcatt ttaattctct
12181 ccccctattt attgactttg acaattactc aggtttgaga aaaaggaaaa aaaaacagcc
12241 accgtttctt cctgccagca ggggtgtgat gtaccagttt gtccatcttg agatggtgag
12301 gctgtcagtg tatggggcag cttccggcgg gatgttgaac tggtcattaa tgtgtcccct
12361 gagttggagc tcattctgtc tcttttctct tttgctttct gtttcttaag ggcacacaca
12421 cgtgcgtgcg agcacacaca cacatacgtg cacagggtcc ccgagtgcct aggttttgga
12481 gagtttgcct gttctatgcc tttagtcagg aatggctgca cctttttgca tgatatcttc
12541 aagcctgggc gtacagagca catttgtcag tatttttgcc ggctggtgaa ttcaaacaac
12601 ctgcccaaag attgatttgt gtgtttgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtga
12661 gtggagttga ggtgtcagag aaaatgaatt ttttccagat ttggggtata ggtctcatct
12721 cttcaggttc tcatgatacc acctttactg tgcttatttt tttaagaaaa aagtgttgat
12781 caaccattcg acctataaga agccttaatt tgcacagtgt gtgacttaca gaaactgcat
12841 gaaaaatcat gggccagagc ctcggccMta gcattgcact tggcctcatg ctggagggag
12901 gctgggcggg tacagcgcgg aggaggaggg aggccaggcg ggcatggcgt ggaggaggag
12961 ggaggccggg cggtcacagc atggaggagg agggaggcgc tgctggtgtt cttattctgg
13021 cggcagcgcc tttcctgcca tgtttagtga atgacttttc tcgcattgta gaattgtata
13081 tagactctgg tgttctattg ctgagaagca aaccgccctg cagcatccct cagcctgtac
13141 cggtttggct ggcttgtttg atttcaacat gagtgtattt tttaaaattg atttttctct
13201 tcattttttt ttcaatcaac tttactgtaa tataaagtat tcaacaattt caataaaaga
13261 taaattatta attgggtgtt accatttttt ccttgatagt gagacgttcc cgagcgagtt
13321 acccatctgc ctgcctgtct tttctttctg ttaagtgact gaatttgggt tttaactctg
13381 gtgttctcgt tgacccttta tgaggcagca ctttcatttt tctccagagt ctcctggtcg
13441 taggtgttaa ctttgggtcc aatctgcctt cccttggctc tttctagatc cgatttcttt
13501 accttctcca ggccaacctc ggggtgtggt Gctgttcatc aaaacaatga acatggagtt
13561 tagagtccag tgagtcaata aagctttttt ttgtgcaacc tgatctaaca tggagatgtt
13621 ttctcttgag taaactcact gagttttcca tcttaaatta ctcagcagtg actgagagct
13681 acctgcatgg aagcaggaag ttactccagt gttataaaca aacttcttag actcaacatc
13741 ctattctcca tccctttttt tttcctgtag agtccaaaaa cctcaaccgt ctcattttta
13801 aattatccaa ttggagttac ctttttaaaa aagttattct taaggacttt ccaatacctc
13861 tcctgaggaa gacatgtcag gtcttctaaa agtttacatt atgaccaaag aaagatgctg
13921 gtcctcaggc attctaccca gggggctgtt ctccaggcca ttcccacctc ctgctaagac
13981 • catggggagc cctctctgta aggaggggca tatgcaggga cctgcccatc cccttggtaa
14041 gctggatggc aaaaaggcat gttgtctgca ccactggctg cctgctaatg tcgctgtcag
14101 tggggaagga gaagtgacaa cacgtttgag tgcatttgct ttgactctta gaaacccaag
14161 cctctgaaaa aagagtaact acttgccagc tgttgttaca gatgatattt ttaggaaaac
14221 atcccgtgac agcaaatcag aattggactc tttttcagag aaatgatttt attgatggag
14281 tacaacctgg gtatttagct tcctttcaac aaaatttttt gtgccccttt cttgagactg
14341 tccatgaatg acagagacat agaataagaa ctggggtgct aaagatggaa taggcaaacc
14401 ccacaccaag gaaatccaca gtgaagtgga aatctggttc ttatgaacat tttaagagca
14461 tctgaagcct gtacttcacc aaccctaaaa taatacgtaa cacgagatga ttcctcagga 14521 aggaacatat agacatacag acaggagaca acatatagac attagaactt acaggacaaa
14581 gaactctttt tccctgaatc atttgagatg aagtttcttc ccatcaccag gcattcctac
14641 aaacaaggac cttcacacga ctaagatgcc acctggtgat taaagaacac tttgggctgg
14701 tccgatccat tgtacaagtt aaagcactga tgggtacaca ggtcttaccc ctgttggcga
14761 tcgggtcaca aggagccagt gtgtgtgtca ccaggaggtt gtatggacga gactggattt
14821 ctggaaatat cctttactga ctctgaagaa tcccatggct cagggaggta cactcatgtc
14881 ctttcttact tctccggttc cactctgttg actagtagtt ggcctcttga gccatacttg
14941 ctgtcagttc tggacattgt tctgtgagaa tggggtcaac gggcagtcgt ggtgcaggtg
15001 agcttgtgtg caaggcggca agatagccac attgagttgg ggacagtatg gcctgggggt
15061 gttggatagg taaggcagaa gcaagcccag aacttggagc ctgttgtgac tgtcacagta
15121 aggagtttga agtcctgaaa gcagttgaga gctgtaggag ggccagattg ggcttttgag
15181 caagatagtc tagatgaaag taaagagatg gagacgcttc agcaaccaga tgggaggttg
15241 ggcatgggag ctgtggctgt gctgagctga gaagcctcag actgcctgcg aggctctctc
15301 tgggaggaag tcaggtcttt ctttccttcg tttccttcct ttcattcctt tcctttcctt
15361 cctttttcct ctttctttct ttccttttct ctctctctct ttttctttct ctctttcttt
15421 cgagatggag tctcgctctg ttgccaggct ggagtgcagt ggcatgatct cggctcactg
15481 caatctctgc ctcccgggtt caagggattc tcctgcctca gcctccctag tagctgggat
15541 taaaggcacg cactgccacg cccggctaat ttttgtattt ttaatagcga cagggtttca
15601 ccgtgttggc caggatggtc tcgatctcct gacctcatga tccacctgcc tcggcctccg
15661 aaagtgctgg gattacaggc gtgagccacc gtgcccagcc aggtcttttt cttaaagagg
15721 gacagactga aggtaggctg tgaatggtca gttatttgta tttctttacc ttttatgcta
15781 tcttgtagtt tgaccagttt gcaaaacaaa ttgaataaaa agtaattgat aatgttgttt
15841 tgatgctcag tgtttgaggg aaacacaatc tagtgaatgg ggttattggt tgtcacacct
15901 gggagagtga aaatgagggg ctcacaaccc aggtgaggtc atctggtgcc actcagcccc
15961 atcccagaga aagcagcctt gggatgctgg cctcggtgac aaccaagaag tgatggtggg
16021 ttgctagaga cagggtggag cacagagccc tggaaggaag caggaagtca ggttcaagaa
16081 aggacttgtg acctcaccct tggcttcagg gttttatttt tctttctttt aaaacatttt
16141 gagacaacat gtagacattc aaacttacag aacaaagaac tattttttcc ctgaatcatt
16201 tgagactaag ttccctccta tcaccagaca tttctacaaa tgaggacctt cacaggacta
16261 aggtgccacc atcagaacca caatatgtgg ggacggtact gccagctaag cctYagtgct
16321 gtttcggtgt tgacagctgt cacaatgatg cccttttcag tagaggagcc tgtgatggtc
16381 acgtgttgca ggtgatcatt gtgccatttg gtttacttct gtctggaaag tttctccttt
16441 ctgtaacctc catgaccttg acacatgagg tcactcttct cacccgcccc acctctggga
16501 gggtgcctga tttgccctgc cccacccagc ggctttagga ctcagctctt caggaacaat
16561 tagggagagg gcccttgggt ctaactgcaa aatcatactt gaactagaaa atcccgttgg
16621 cataggtatg gattgtcata tagaaacatt tttcgcagtg tgccccgtgg aacacttgtt
16681 ctgtatgttg ttaacagatg ttttgcaaaa aatgaaaagg acctgagctc taataagttt
16741 gggaaatgct gaattgaagt gaaacagctt ttcttggtgt gagacctttc agagtcttta
16801 aaggagagtt gctgaccaca cgtgaaatca caatagaaaa tggccagcag gcctccaggt
16861 aggcagcaat tcctagacat ttgcctgtgt tccctggggg agctggtggc ctgtgaccat
16921 ccactgggaa attcccttct gtggcgaggt tctgttgttt tccagaggct cccacggagc
16981 atgctgggtg ggtggtctgt atgagaccac accgttgatg agcagtgagg cccaaacagg
17041 tatatcaaaa aatgctcagc atcgctgatc atcagggaaa tgcacatcat aaacacagtg
17101 agacgttgcc tcacacctgc cagaatggct actgtcagac gaaagacaac aagtgtgggt
17161 gaggatgtgg agaaaagcga acacttgtcc acctttggtg agaatgtaaa ttggtatagc
17221 cattatggaa aacaggaagt tcctgaaaaa attaagaaca gctaccagga atcccacatc
17281 tgggtgtata tccaaaagaa atgaagtcag taccttgaag agatctctgc agcccctgtt
17341 cattgcagca ttattcacaa tagccaagat agggaaaaag cctcagtgtc tactgacaaa
17401 taaagatgga tcaagaaaat gcatgtatgt atacacacac acagtggaat accaagtctt
17461 gcagaagaag gaaatcctgt catttgtgac aacatggatg agctgagaga acattatgct
17521 aagtgaaata agccagacac agaaagaaaa ataccagatg atctcactta tatgtggaat
17581 ctaaaaaggt tggactcaga agcagagagt ggaatggtgg ttaccaaagg ccagagggtg
17641 caggaagtga gcagatgttg gtgaaagggt gcagaggttc agctatgcaa catgaatgaa
17701 ttctggagat ccactgtgca gcatggtgac tatgatactg ctttgtgtat ttaaaatttt
17761 cagagagtag atcttgtgtt ctttcccctc tccaaataaa aggaaattat ttgaggtgat
17821 ggatatgttt aactagcttg actggggcaa tgattttgta atgataagta tctcaaaaca
17881 tcatgttgta caacataagt atacaattga cccttgaaca atacaggttt gaactctgtg
17941 ggtccactta catgcggttt tttttttctg taaaagttac acccgatgcg tcagcccctt
18001 cttctacctc ttccacctct ggcacccttg gcacagcaag accaaccccc cacttcctcc
18061 tcctcctcct cagcctactc agggtaaaga caaggatgaa gacctttatg atgatccact
18121 tccacttaat atataataaa tatattttct cctcatgatt ttcttaaaac tttttctgta
18181 ttttattgta agaatacagt atataataca tacaacatcg aaaatatgtg ttaattgact
18241 ctgttattgg taaggcttct ggtcaacagt aggcaatact agttgttaag ttttagggga
18301 gttaaagttc tatgtgggtt ttcgattgtg tgtggggtct gtgctcctaa tccctgggtt
18361 attcaaggtt attctacata atttttctgt ccgttacacc tcagtaaagc tggataaaag
18421 cgaaaaccaa aagactacga aggctaggag aaaatcagta catgccactc cagcccccat 18481 tctccccaga agcagtcccc acttcttccc ctcagctttg tcccctgtca gtttaaaatc
18541 atgaggttca taaatttgaa aaggaaagct ttatttcttg taaagggtct cagcctgcaa
18601 aatcgccatc ctggcaggct gggaagtgta tccagcagag accaaaggca ggtacttgga
18661 gacggggaag gatgaggcag gaatttatgc tgaatgggtt ggccaagcat acatattcaa
18721 caggtgatgg ggggagctat gaatattcat gacaggggga catgcacatg cattgtaaac
18781 attcctgtta catacgcccc atgatcactt tggggtggag acttaacatt taaatgcatt
18841 tcaattagga cttatgtgtc aaaaggtgaa gcagggacat gaacgccctc agtgcatgcc
18901 tgggcaaact agacaaccag tccatgcttg gtggtctttt accaggagaa agtccctgaa
18961 atcaggctct tgttaaagct gtagttacag ctggtggaga agggggtcag ttagtctgca
19021 tctggccata ggtggtgagc tgaaattgtt gccgtattgc tgatctccag gcccctgctt
19081 atttagctgc cagagaaaaa gaaaacaaaa caaaactttg tggcagttag aacctagttt
19141 attttttaag tgcctgactt aactcttgcc tggcatggcc ataggtcatg tttataattt
19201 ggcatcttac ttccacaaag agtctgttct gtcgtgttat gatctgtaat tttaacacca
19261 ccaacagact tctctctcct tcaggtcaaa ttctccaagt taaccttagc attgatgcag
19321 ctgaaatact ggagccattg aacggacact tctgacctgt tcctacacta tcctcccacc
19381 tttgggtgct gaggtagatt gctatgggtt gaactatatt ccccaaaatt cacatgttga
19441 actcctaacc ccagtacttc agaatgtgac cttatttgga gatagtgtct ttaaagagat
19501 aattaagtta aaatgagatc atatgaatgg gatgtaattt aatatatgtg tctttataag
19561 atgaggagat taggacgcag actcacacag aggcatggcc aactggaagc caaggagaga
19621 ggcctcagaa gaaagcaaac ctgcccacac ctggatctca aactcccagc ctccagaagt
19681 atgagaacac aaattgttta aactacacag tccatggtac tttatggcag cctaagcaaa
19741 ctagcccaga cacactcagc aaccttccca ctgccagtca cacgatgcgc gcacatacat
19801 gctgggcact ccccaaggcc ctgtgcctct gggagagtac gcagaacccc atgtcatgat
19861 tcctcccatc ggagctgaag gaagccagaa catgctgacc tgcaacatgc tactttagcc
19921 taaggattat ttggcactgg aggcagttaa gaagcagcaa atgtatttgc ctaaaagcaa
19981 gacatacatt tttcaaaagt gtctctcctt ccctctctga acaagagtta atcacaaaga
20041 cagctccaga caccgctcag cttggagatg gcagcagagg tgttcacgta accatcctca
20101 ctaactcgcc ttctctaccc caacctttcc acttggtgca gacgccttcc tgcagcgccg
20161 tctctggaag cttgaagtca ctttcctgtg tcccagcatt tctctacaaa taaattgttc
20221 ttagtgaaga tggtacgtat gccagagcgt gaagcctatc tttcaattgc tgtttcctga
20281 atttctctca tgtctatatg agatgtacat gctagaaact tgtttttctc ttattaatct
20341 tttaattttt ttttataggg gccccagcag aaaacttaga agagtacaca gaataggatt
20401 ttttccttct ttacaaagtt ccgtgtagga aggcatgagc atttctcttc ctatcacaag
20461 tgtccatgcc atggtgtgtc cggaattggt gggttcttgg tctcactgac ttcaagaatg
20521 aagccgcgga ccctcgcggt gagtgttaca gctcttaagg tggcgagtct ggagtttgtt
20581 ccttctgatg ttgggatgtg ttcggagttt cttccttctg gtggattcgt ggtctcgctg
20641 gctcaggagt gaagctgcag accttcgcgg tgagtgttac agctcttaaa agcagtgtgg
20701 acccaaagag tgagcagtag caagatttat tgcaaagagc gaaataacaa agcttccaca
20761 gtgtggaagg ggacccgagc gggttaccac tgctgactag ggcagcctgc ttttattctc
20821 ttatctggcc ccacccacat cctgctgatt ggtagagctg agtggtctgt tttgacaggg
20881 cgctgattgg tgcgtttaca atcccagagc tagacacaaa ggttctccac gtccccacca
20941 gattagctag atacagagtg tggacacaaa ggttctccaa ggccccacca gagtaactag
21001 atacagagtg tccattggtg cattcacaaa ccctgagcta gacacagggt gctgactggt
21061 gtgtttacaa accttgagct agagacagag tgccgattgg tgtatttaca atccctgagc
21121 aacataaagg ttctccattt ccccaccaga ctcaggagcc cagctggctt cacccagtgg
21181 atcccacacc ggggctgcag gtggagctgc catgcgcccg cactcctcag cccttgggtg
21241 gtcgatggga ctgggctccg tggagcaggg ggtggtgctc atccgggagg ctcaggccgc
21301 acaggagccc agggaggggg tgggagcctc aggcatggcc ggctgcaggt cctgagccct
21361 tcccggcggg aaggcagcta aggcccggcg agaaatcgag cgcagcgccg gtgggctggc
21421 actgctgggg gacccggtac accctccgca gctgctggcc cgggtgctaa gcccctcatt
21481 gcccgggccg gcagggccgg ccggctgctc tgagtgcggg gtctgccaag cccacgccca
21541 cccggaattc cagctggccc gcaagtgccg cgcgcagccc cagttcccgc tcgcgcctct
21601 ccctccacac ctccctgcaa gctgagggag ctggctctgg ccttggccag cccagaaagg
21661 ggctcccaca gtgcagtggt gggctgaagg gctcctcaag tgccaccaaa gtgggagccc
21721 gggcagagga ggtgccgaga gcgagcgagg gctgtgagga ctgccagcac gctgtcacct
21781 ctcaatggta cctgggctaa aatagacatt caggaccatg tttttgaaca gttaggaaaa
21841 tgagtggatt ttctgcatca aattttcagt agttcccagg aacagttagc ataatgggcc
21901 acaagggcat gcagggtggt gagtggatgt ggtgggggcc gcctggatga caggcctggc
21961 ctggggctcc tcatggccgg aagtcagcct ccctgtgtta gctctgcccg caggtcctgg
22021 gcctcatctc tggatttgtt tgggagggct gccataataa agtaccacag actaggggct
22081 tcaacaacag aaatgtatgt cttccagttc tggaggctgg gagtccaaca tcaaggtgtt
22141 ggcagggttg attccttctg agactgtgag ggagaatctg ttccaggttc ctaggagcac
22201 agcgttcccc ttctgacagg cacaaagcag gaaacagcca accctggggg cctgttgagg
22261 aggacagaag cctaggacac actgcggggc cctgagcaca gccccatggg gaggggagtg
22321 gcaggagcag gagtttccac cccaggcagc tgccccctct gcacctggga ccatcgggtg
22381 ggcagcaggc ccagtgccca gtgactgact tgctcattca tggctgattt tctcacacat 22441 atgtgcttcc ccagacctcc atcatggccc ttggggccag gactggtctg ccctgtgtgc 22501 tgacagccac ttcactggca ggtacctgga ttcatgtaca aatctgggcc taaacccagc 22561 tcttgccttc tctctgtttc caggtgagac ctgcctctgc tccggctctg gcagctggct 22621 tgatcccctt ctcctccctg gccttagggg gcactgagac ctaggacaga tgagatgccc 22681 acctggagtt tgtgtctagt tggacattag gtgaatagga tttcagcata gggagttttg 22741 gaaaatcagt ccctggtgac agggctttga gtgagttgat ttattaagac ttgacataga 22801 gccaggcatg tgattctggg cagatgactt gcccccttag gccttcgttt ctcatttctc 22861 atctgcagta cgtgggggtg agcagttcca tcagatcagg agacggggct cagaaggagc 22921 ccggcctgcc atcctagatg ctcaggatgg tggcaacggc ttgttctcag ttggccatgg 22981 ccctcagtcc catctcccct ttgcaatttt gattgcctgg gatgtctggt catcccctca 23041 tggacccaaa ccatggggct gggagacagt ggaaaaggaa ggatgcaggg gtgggtacag 23101 gctcccttgc tccctagtcc aagccaaaga aaggaggcct gagtccattg agcacccaga 23161 gttgggggca gctgctttct ccatgaaaca ggtgagcagt ggcaccaggc ttggcctgga 23221 caggccactg gggaggtggt accacagagg ggcctggttt ctcctcaaag tgtgctgaaa 23281 gtgggcccct tgcaccctgg ctgcttccca tccacatgca ggacactcgt gtcagggttg 23341 tgggcacaac agggccagca ctgagtgtgc gccaccacct tcctctgggc tggggagtgt 23401 ctgcctctgg cccacctccc tcctgcccag ggcatgctca gccttctggg tggcagcccc 23461 cttgagccct ctcttggcag cagctgagca tttccaaaga agaaaacccc ccaggagtgg 23521 gaattatgga gctgcagccc ccaaggtggg gcatgctttt gaccaaatca gccagtgggt 23581 ctactgggct tgaggctcag ctgagagtct cagaaatcca gagtatgcct gacaccatgg 23641 gcctctgttt atctgtactt ctatgtgcat gtggaggggc tcacccccaa tgccacatgc 23701 actcctaggt ctgaaaatgg gggagggatg gggtgttttc caccttgcgc cactttgatg 23761 ccttccatca gcaactctga gccagagcct actggggcat tacccctgtg cagaggtaac 23821 tccctggccc aggttcccta ggaacttggg ctattgcacc atcagcgagg agtctgccca 23881 ggggagggga ggcagtgagt ggcacctgta ggagctcggg taggttctgg agagcacagc 23941 catctgggtc attgacaagg tgcaggcaat gggcctgctg gagagtgctg ggctgctcag 24001 tgtggggcag ggaggagccc tgcagctgct ctgggattcc caggagcctg aagctttctt 24061 gtctgttgcc gtgacaccat gtcaccacaa actgagctgc gtaaagcatg cacactccat 24121 gtctcctggg ctctgtggtc aggagggtag ctgcagggca gctggatgtt acattcaagg 24181 tgttagctgc agtggtggcc tcgtctcaag gctcatctgg agaaagattt gatttggagc 24241 tcaagtggat atcggcagga ttcattctgt cccacttcag gaccaagggt cttggtttcc 24301 cgctggttgc cagctgaagg tacccacagc tccttgccat gtgggcctcc accacagggc 24361 cagggctcca tcacagccag caggggagag agactctcag ccatgtggat gttacaatct 24421 taaggagcat gatcatggag gtgtcatccg tgacacttgc catttccatt ggttacaggc 24481 aagtcacagg ttctgcacac tcagctgaga ggtgaggata tgtgggccac tggacagcca 24541 gtctgctaca ccctctggtc ttctttcttg tctcctgctc ttccctccac cttgtaaggc 24601 actttttgRa tggtaggact ggttttgggg tcctgtggtg ccccatgcct gctgctgtcc 24661 cagctttggt gactgtctct ttgttacagg acctttgagg tgtcaatttt ctggctggaa 24721 acctctgtgg tcacggtgcc tttgcatgag ctcttgtcct gcgtccagga agaataaggg 24781 acatagacaa gtgaagggtg agcaagacga agatgagctc tattgagtgt tacaacagct 24841 cagaggacac ctgcattggg tagctcctct tctaggcagg tcatctgtcg agtgttcagt 24901 tctcagtaga gaggaggccc aggagaggta gctcctctct gcaactggtc atcctgatgt 24961 ttgcagctct cagcagagag gaagccctgg agagggtggt tcctctctgc cagcaggttg 25021 tcgctgcagc tatcagcaga gaatgtggct cctctctgtg ggttgtccca tcatctccag 25081 ctatcggcag agagggtact cctttctgca gctggttgtc ccatcatctc tctgccctct 25141 tcatcctcta gctatcctct gccctgctct ggctgagtcc agggctttta tggacctcag 25201 aagggaggaa gtgcatgcca gttgttctgt gggtggccat gggtgggtta ggaagaggca 25261 ccacaagtcc ccactctggt ttccgggact ggcagcccag gccccagcct tcaggccctt 25321 cttggcctga aggtgggcct tactagggac acacctcctt cttcccagga ctctgtctgc 25381 ctcctgctgc cattcatggc cccaggtctc aaccccaacc ctgctctgag atcggagcag 25441 gtgctgggat gggagagggg ccaggcagtg ggagcagact cttctgagcc tgggatgggg 25501 actagtgggg aggccttccc aggcccctga ggttgcaggc tgcagagatg cccaggtctt 25561 gtgcctggga gggcagccac agctgcatcc acggaactcc caccctgcca actcagaagg 25621 ggtggggttc ctgcttgtcc ctggctcctg cctgctctgt ggagtgagag gcccaggtct 25681 gcagccacag gtcaggtggt tgcagctgca tccaggcagg cagatctggc ctgctgctgg 25741 ccccctccaa gagcacaagg aggcttggat ccacagtccc aggagggtgg ggcttccatc 25801 agctccgtgg agtgttcagc cccagccacg cctccctgct gctgccatca tttccccctc 25861 tgaagaggta cacctaactg ctgttagggt agggacaatg accactctta actgcttcgt 25921 gctgaccagg gatattgttt tgggaaaaca acagtcaagt ctctctcaga gacctatcta 25981 agaatatcca gtacaaggca gccatcatct gaggctccat tttcatgacc atttggagtt 26041 taacggccca tcccttgttt cttctgagct gtagtcagat atcactagtt ggttcacctt 26101 cacaattgtc aaaagctaca aatagctcaa aagaaaagct tccttgattc tgaaaaacaa 26161 gataaaggat caacagtatt ccaagcaaaa ggtcaaaaca agattgcttc agtcttctat 26221 tagttcagct cacccagtca actcctattc acaatctcca aaattatcag aaatctgcat 26281 ttgaagacta taatccatcc cttgaagagg atcaaaacat gacaacaatt gtctgtgaat 26341 gtcaaaatgt cctagcgtat tcatagtcaa aaacacaatt gatgaagaaa tctggtcacc 26401 tctgtgattt acaataacct aacacaataa ccctagctat gattgataac acaaactcag
26461 gcatcagaaa tctagaaatc ccatacaatt ttgaaacaca cattaacatt tttcactaaa
26521 atataacctg aagatcaaac accttatttt ggtaatccta tgtaactaaa cttgtcaaat
26581 aaccctgttt acctctccct ttcgatgctc caggtgtctt ctgtagcatc caaaactcag
26641 gggttaggaa agaaaatttt gaagctgtaa aggctcaaaa cacttaacag aatttttggt
26701 tactataagt cattcattta aatgactcaa agcaaaaacc ttcagtcact aagagggaag
26761 acctaatttt ccaaacaatc taacttttaa ccatcgcact cctttttaag aagtcctttt
26821 aagtctctta ttatccaact ttagccatgc caaatggcca gttgaattca tggaggggca
26881 gagaatatag tgattttttt attgttcctg caaccagttt gcacagagag agaaaccaaa
26941 agtctgactg gtaagaactt ttaccctttc gccagcatgt caggctgctg agctcccttc
27001 ccctcagcta tggagcccta ttgaccctgg agtcctgttg atctcttgtc cttcccttct
27061 tgtgtcagta gcttattcac aaagaaaaca aaatctttca ttgcatgaaa atcttgttca
27121 agtgaaagcc aaattttacc cttacattag tctattaatg tcaaccccaa ttaaaaaaaa
27181 taaaacctta cagagaaatc catccaatct taatcagttt gatcatgaac tgagattgtt
27241 ataaatcttt tataaccttt tacaaatttt ttttgttaaa cagcatgtaa gtgcttcaag
27301 aaaaccttgt tgtgcttgta ttccattgtt caacttatgg aaaaccaaat aatatccttt
27361 ttagtgtagt caatatgttt acacacagga ttccttttac aagattaatt ttttacaaac
27421 cttccataac ttgtttgaac cttaagcttt atcttatata atttaaaaca atcatttaac
27481 cctctgaact aggcaaaaat ttacattccc atgccttctt atattatttt actaatgata
27541 cattttactc tccttactca cctcacgtgt agatctgttt tcagtagtca tcactacatg
27601 ttataatggt agctcttagt aattgttaat tttggtgaaa tacccagtaa gttattttag
27661 ttacgtactt aggtgcagat gaggtctaac tatttccagc atagctaggg caggccctac
27721 caaactgcaa agcaggaaag ttgaacaatt ttcaaaagcc aaagcagttt atgaccttaa
27781 agcatttagt caacctagtt tctgacttgc ataatttaga ccatgtctat attttgaaga
27841 catttctatt ttcattttac tgataattta aaagacagcc tatttagcaa agtcatactt
27901 aagtgaattt gaaaattgct tagacttatt tacttaattt atgaacactc ttttacttat
27961 aagccaattt ggtagacaca acatataaca gtaagtgtac atacaagtaa acacatctag
28021 acatgtatac acacacacaa atgaagaggt ggagcttaaa acacccaaat tgggtgcagt
28081 gtatactgct tgggagatgg atgcaccaaa atctcacaaa tcaccactaa agaacctgct
28141 catgtaacca aataatacct gttccctcaa aacctatggg aaaaaaaaac taaaaaaaat
28201 acttaaatgg tatcacagaa ctaattagcc gaatacagta tctagtacct ggctgtcacc
28261 caatacttgc ctcataccat cacatctaga aaacaagtag atattctttt tggaagagcc
28321 ctgagggagc tactaggagg tttgcacggc ctgctctcct gccctcttct tgctctgtgg
28381 ctgaacttca attctcttcg ggcttagacc ccactgactc gctcccgggc aaagcaaacg
28441 atttgatcag atggccacgt gcattcttcc ttttcctgaa accagcacca tagggtaaaa
28501 gattatttct acttggttgc cttccagatg tttcacactt ggacagcaaa ctgatttcaa
28561 aocactcctt ttcaaagatc tctgagggag acattgcacc tggccactgc agcccagagc
28621 aggtctggcc acggccatga gcatgctgag ccatcatgcc caccgtggat gacattctgg
28681 agcaggttgg ggagtctggc tggttccaga agcaagcctt cctcatctta tgcctgctgt
28741 cggctgcctt tgcgcccatc tgtgtgggca tcgtcttSct gggtttcaca cctgaccacc
28801 actgccagag Ycctggggtg gctgagctga gccagcgctg tggctggagc cctgcggagg
28861 agctgaacta tacagtgcca ggcctggggc ccgcgggcga ggccttcctt ggccagtgca
28921 ggcgctatga agtggactgg aaccagagcg ccctcagctg tgtagacccc ctggctagcc
28981 tggccaccaa caggagcoac ctgccgctgg gtccctgcca ggatggctgg gtgtatgaca
29041 cgcccggctc ttccatcgtc actgaggtaa aaaagcctct gtaacatggg agttcctggg
29101 acagggagaa atataaagca aatcctatga agttcagttc catatcagga aagaggtagg
29161 gggctatgtt tattgtgcag ttcgtgggtg tctggctgtg tcccaggcac ggggcattct
29221 cttctctagt ttaatccacc acaaactctR ggtctttgag gaggaagctg aggcaggaga
29281 gaacagctag cacaagtcgg gggccaggtt gccaggttgc ttgaatggga gttgactggg
29341 agaccaggag cctctctgcc tgccccactg gcttctccca gaactatcaa cttgctctgg
29401 gtcccgcttc cccaccaata gtactgtggg ccccctcctt ctgctcaaga gtgatgacga
29461 ttgacctcaa tgatggtgtt gaggtggcct ggaattgccc tggcttggaa gtgagaatat
29521 ttggtggaat cccagctgtg ccccttatta gctgagcatc actgggtaag tcactgcctc
29581 tctaagcctc atttcctcat cagtcaacca gggatgatga cggctgcctc ataggggtgt
29641 gagcttcagc tgggatctgt gaaagcactg agcaaatcca aactgagtca caccgtgcag
29701 tccgatgggg cccatgggat agggcgatgg cctcaaaagg gccttgottg agtagtttac
29761 ttggttattt gagcattttg ctttgagaat cctttagtaa aaagtgtcac cacatttttt
29821 tcccatcatg agcccttttg aaactattgc acggtaacaa gtgtggatgc ttggtgggtt
29881 ggagaagctg tgggccaaag cccgtcaggg gccttctgtt agtaacgagc agtggcgtgg
29941 ttttggtgca ggcactgctt ccctgaaata agaggtaact gggccctccc tgcctctggt
30001 cctgcccact tgtctgcccc cactaccctc gcccaagctc tccggacttc ttgggtctct
30061 gttcttcctc ttttcccttt cctgagtggt atcagcggtg acacatttag ggctattact
30121 ggaggaaagc ttagggtccc tcccctctgg gcctgagcat tgctctacct gcttgcctgg
30181 agcagagcag gtgccggtgc aactggcttt acgatgctga agagacatct tgtctccaag
30241 gcatcctcca acaatggatg acaagttagg cagcccacct tccagggatc tccaaccatc
30301 tgacattccc actgggaatc tagaagcaga ccaggatgag gatgtgtttg ctctctggga 30361 ctgagacata ctctgctttg aggagatcag atgtgatcat tcctccagtc agacatccac
30421 ccagtatgtt cggcatactc cctctattct ggcactgggt gtgatgccgc aggggagatg
30481 cagaagaaga ttgtgaggtc cctcaaggag tctgcagtct tgtggggact gagaggggaa
30541 gaggatggca agaatgagac cagtgttcgt ttaatgacag cccaaggccg cctgtggtga
30601 atgccatgag cagggcggaa agggactggt gggctctaag aaggtagaga tctcagctgc
30661 tgagctctga gctgatcata gacgtcttct gggaggaggt ggctttgagc tgagcctcag
30721 ctggtatttg gaccagcaag gagaggggac aggtggagta gaggggaggg gaagtagatc
30781 cccagaccaa ggggagagct tgagcctagg ctcagggccc agggagtgtt tgctgcattc
30841 agggtgcaag gagcactgat atgagggtca tgatatgagg agttgtgggg agaacactgg
30901 tgtctaacct tggggccaga acacggagtg cttagtgtgg ggttgccagg tacaatacag
30961 gatgcccagt taaactcgaa catttgaact tcagataatc caactggata ggatacttac
31021 ttcccagata ctgcatgata tttggtactg ataactgaga ctggaaatgc aaatattgca
31081 tgaacatact tatcctaaaa aaaaaaaagt tgtttatctg aaattcaaat ttacctgggc
31141 attctgtatt tttatttgtg aaccctggca acgctacctt agtgcccaac aaaggagttt
31201 gggtcttatt taggcaggca agcaaggtga ccctagctgg actcgtgaat gggtgggaag
31261 gggcggtggt gggattggag gcttcaggag aggtagagag atgcatgagg aggccaagag
31321 tgagccccgg taagaagtgt tggccaacat ggttgtcatg ggaatgaagg gaagacaaga
31381 tgtaagagat gccagcattg tcagggctgg ggagaaggag gcccagcttg gcggagggct
31441 ctggcttggg gggtataggc aaatggagct tctgaacgct gaactgggag ccttggagag
31501 gcagccatgt gggcaggtag aggggcctgg taagagggga ccttggggtg cagcagaagt
31561 ggggaggctg ggacagagag gggcccgaga gtgctttaga acagcttgcc tgtccgtggc
31621 agctgtggag ggggcttcca ataaccccaa gatgtagagc cctggggagg attgtgggaa
31681 tcctggaact ctgatgggca gagatggtga agaggatatg gtttgtattg tacctggcaa
31741 ccccacacta agcactccat gttcttttgg ggtcgtggta agttgggagg gtggtgggat
31801 tcatgtggag attttccaag gtgattggaa gcctgggggt aggtgggagg gtgatagaaa
31861 ctggctcgtg ggtaagaatt gtctcccact ttaaccccaa cttccttggc cccagctcct
31921 cctccaaaac tgaaaattat tgttggggaa tcaatcttag ttattcattt ctgcagaact
31981 aatttttaac ctagcattgc cttggacatt ggaacttgct ttaggaggga aggtgagcta
32041 atctgttcga acgccaagtt taactcccac tgcaaggcta ggtgctaaga tcatgaggtc
32101 cactYggggg ggatgtgggc attttcctgg cttttgactc tcccaacaag aggatctcct
32161 ctggtgctaa gatcatgagg tccactcggg ggggatgtgg gcattttcct gacttttgac
32221 tcttcccaac aagaggatct cctctggtgc taagatcatg aggtccactc agggagggat
32281 gtgggcattt tcctggcttt tgactctccc aacaagagga tctcctctgg tgctaagatc
32341 acgaggtcca ctcggggggg atgtgggcat tttcctggct tttgactctc ccaacaagag
32401 gatctcctct ggtgctaaga tcatgaggtc cactcgggga ggatgtgggc attttcctgg
32461 cttttgactc tcccaacaag aggatctcct cttctgctaa gatcatgagg tccactcggg
32521 ggggatgtgg gcattttcct ggcttttgac tctcccaaca agaggatctc ctctggtgct
32581 aagatcatga ggtccactcg ggggggatgt gggcattttc ctggcttttg actctcccaa
32641 caagaggatc tcctctggtg ctaagatcac gaggtccact cgggggggat gtgggcattt
32701 tcctggcttt tgactctccc aacaagagga tctcctctgg tgctaagatc acgaggtcca
32761 ctcgggggag atgtgggcat tttcctggct tttgactctc ccaacaagag gatctcctct
32821 ggtgctaaga tcatgaggtc cactcggggg ggatgtgggc attttcctgg cttttgactc
32881 tcccaacaag aggatctcct ctggtgctaa gatcatgagg tccactcggg ggggatgtgg
32941 gcattttcct ggcttttgac tctcccaaca agaggatctc ttctggtgct aagatcatga
33001 ggtccactcg gggggtatgt gggcattttc ctggcttttg actctcccaa caagaggatc
33061 tcctctggtg ctaagatcat gaggtccact tgggggggat gtgggcattt tcctggcttt
33121 tgactctccc aacaagagga tctcctctgc tgctaagatc atgaggtcca ctcggggagg
33181 gatgtgggca ttttcctggc ttttgactct cccaacaaga ggatctcctc tggtgctaag
33241 atcatgaggt ccactcgggg ggtatgtggg cattttcctg gcttttgact ctcccaacaa
33301 gaggatctcc tctggtgcta agatcatgag gtccactcgg gggggatgtg ggcattttcc
33361 tggcttttga ctctcccaac aagaggatct cctctggtgc taagatcaYg aggtccactg
33421 gggagggatg tgggcagttt cctggctttt gactctccca acaagaggat ctcctgtggt
33481 gctaagatca tgaggtccac tcggggggga tgtgggcatt ttcctggctt ttgactctcc
33541 caacaagagg atctcctctg gtgctaagat catgaggtcc actcgggagg gatgtgggca
33601 ttttcctggc ttttgactct cccaacaaga ggatctcctc tggtgctaag atcatgaggt
33661 ccactcaggg agggatgtgg gcattttcct ggcttttgac tctcccaaca agaggatctc
33721 ctctggtgct aagatcaYga ggtccactgg ggagggatgt gggcattttc ctggcttttg
33781 actctcccaa caagaggatc tcctctggtg ctaagatcat gaggtccact cgggggggat
33841 gtgggcattt tcctggcttt tgactctccc aacaagagga tctcctctat cagtcttggt
33901 ttcagccaca gcccccacac caggccagct gtcttccacc agccatgagt gctgagcaag
33961 ggaaagtggt ctcaggccaa gggagacaga ggtagttaca ttagtgtttc tcaaacttgg
34021 ctgatcctca ggatcacctg gattcctatt cgggacccac tgaggcagaa tacacaggga
34081 atctgtggtt ccaccaggac cctgtgtggt tctcgtgatg Ragcagtagg ttgctttata
34141 atcgtgccgg aaagagctgc cccggccctg gtgaaatccc agcaggagca gcgagtgggc
34201 ttaggagatt cccatcctcc atcccgtccc tacctttatg cagccccagc ttctcccagg
34261 gcccagccta tcttctcaag gtgcacaaat gtgtcagggt actcatggtt gggctggtgt 34321 ttgggagcag aggaagcatg gcaggtttac aagctttgca ggtggctcta gaaactgatc
34381 ctcagggtag cctggggcag agccagttct ctgtgtggat gagatgttgg aaggtgggat
34441 gcactccagc tccaggagct cactggttcc atatacagtc ctggggcatg atggcaacag
34501 ggtgtttcag gaaggcggca ggcacatctt acagagatac caacttgcaa agattataat
34561 aaaaaccaga caaacaatgg ctgagaaggg aataagccga ggccccaggt aataattcca
34621 catctttccc tgacccctgc aacttgagtg aaatcctgta cggcagacca cgggtgaggc
34681 tgcaaagaga gagttgggca gttggagaca caaaccctgg gaaagccaga taaggacgtg
34741 tccacgtgta ttctaatggc agagcacacg gcaggcagga aaagaaccac agccaaaaag
34801 agttatacat cgggtgggtg acctgggagg cgctccatgg agaagctgtg ttttaagctg
34861 aattttagag gaaatcaata ttgggattgg cagaaaacat gagagtgaca ctgagcagta
34921 gcttgtcgca cgccccagag ctgtaccacc cataggagca gacactgggg tctgggcaga
34981 aggcagttac taggagaggc ttgtgtttgt ttctgaaact caggtggtaa cacagagttt
35041 aacctatatt tcagggaaaa aaaaaagtgc tcatgtcaag gaactagtca gtccatcaga
35101 cagaacccag gctggctgag ttcagagtcc ttgaggcttt gaatgttgtt tggtttttga
35161 gacagggtct cacactgtca tccagagtag agtgcagtgg cttgaacaca gctcactgca
35221 gcctcaacct cctgggctca attgatcttc ccaactcagc ctcccaagta gctgggacta
35281 caggcacatg gtaccatgtc tgactaattt tttaatattt tgtagagatg aggcctcact
35341 ctgttgcccc agcctggtct caagctcctg ggctcagacg atcttcctgc ctcagactcc
35401 caaagtgctg ggattaaggc ataagccact gcacaaagcc agttctgaat gtttgacccc
35461 aggatcgggg catggtgtgg ctcaaactgg caactttaga gatttgaggg ttggaagtgg
35521 gggtaataaa acctgtttct aaaataaaag ggtttattat ttattaaagc tatctgcatg
35581 tgatttctcc cctccaatgc acccagcagc cctatgggtg tgatggaatg ggtacaacac
35641 acgtttcaga cagggaaact gaggcccagg gctgtgaagt gactcactgc catcgtactc
35701 cacagtattc tggttagggg agccatggac ttgatgcaga caaagcagtg tggagggacg
35761 ggggcaggaa ggatgtgtcg accacccagg aggggctgta ccaggaactg aaagggtcga
35821 gaggcctcca cccaacaccc tctgttctcc ccagagtcca ctctttctgc ctgtctcaac
35881 tctaaagagg aaggggccat tcttctgccc tccagcctct accatgtcta ggcatgtgcc
35941 agcatcacca tgctctgtgc tatgggaggt aggactcttg gatgtccctc cactcctggt
36001 tttcttcccc aaggcctcta aacgcaggga atatctgcct tctccaaaaa cccaaacaaa
36061 acaagccctt cccagcagcc catgcctgtc ttttcctctg tcccctccac cttgatatct
36121 tccctcccct ggcttttggt gcatgggagc ctctgatgtc ctcctaacct ttggtcccac
36181 ccacatgtga ccataaggca cagtctatct caagaaactt aacaactaat agcttactat
36241 tgactggaag gaagctttgc caatcacata aacagccaat gaacacatat cttgtatgtt
36301 atatgtatta tacactgtat tattacaata aagtaagcta gagaaaagaa aatgttaaga
36361 aaaacttaag gaagtggaaa aacatcacga agtggaagtg aatcatcatc aaggttttca
36421 ccctcatcat cttcacgtgg agttggctaa ggaggaggag gaaaagaggg cttggtcttg
36481 ctgtcctgga gggcagaggc agaaaaggtg gaagaggtag aagaagagac aggcacactc
36541 ggtgtaaatt ttattgaaaa aaaaaaatct gtaagtggac ccgcgcagtt caaacccatg
36601 ttgttcgagg gttagttgca tttgatattt gctgagtgat tcatcagaaa ctttgaattt
36661 gtcttatgaa aatgtagtag gagaaaactc tgtgaaacag cccagggata ccgagtttga
36721 tgaactgcat ttgctttgct gaagagagga tggaagggtg tagtcctgac tcacacatgg
36781 ttctgtgctt ttcgtcctcc tcttgccgtg gtatgactgg cagttcaacc tggtgtgtgc
36841 tgactcctgg aagctggacc tctttcagtc ctgtttgaat gcgggcttct tStttggctc
36901 tctcggtgtt ggctactttg cagacaggta tgtaaaggcc agtccaggta agcctcctct
36961 gaatgtcatg agaacagatt ctaagggcga atctgttctc agtggtggag aacatgacca
37021 gttggaatta actgcagaag ctgctggaga caagacacag tggcccctgc tttgggatac
37081 tgtggggcta ccaggggagt ggtggagaga tttgaggtgt gtttcagagt ccaagctgca
37141 tgcagattgt acagttagtg ggctgagaga aagcggggac acagccaaga ttgatgcttc
37201 cagcaggtgc ctggtcgtga ggccattttg agatgggatg cagagggtta ggaagggatt
37261 ttgttgcagc ggttgatagg ggagaggtta tggaggaaac tagggctctt agagatgtta
37321 ggtttggaga tgagttgcac ggcctgagac atctacgaag agatgccaag gagccacttg
37381 ggtatgtgag tgagctctgg aaaggggtct gggctggagt tcaatatcgg gggtttgtca
37441 ccagtagttg gtatttaagg ctgtacagca tatcaggatg tgctggaaga gggtgtcgag
37501 agagaactgc aatgcagaaa ggtaggcagg gaaagtgccc tgggaaagga gacaggaaaa
37561 atggcagcga gatcgaagga caactgttct ccagggcgct tgtggaaatg caccaggaga
37621 ccacgaggag gagtggtcag aatctgctgt ggggggcctg gcacccagga cagggtggtt
37681 ggagaaaacc cgaaggcagg agagtggagt tgtgtgaaca actctttaaa agagtgtggg
37741 gagggtccag gatgaggagg ctgatagggg agggagtcag ggatgcagga agaagacagg
37801 ggccagggaa cccaggcctg gctgggggtc tgaggcccag ctcagaggtg gagacagaga
37861 gaagggacag agagccaggt gctgccgcag gagacaggga gaggggacag agccaggtgc
37921 tgccccagga gacagagagg gtacagagcc aggcgccact gcaggagaca gggagagggg
37981 acagagccag gtgccgctgg aggagacagg gagagaggac agagccagtc actgccccag
38041 gagacaggga gaggggacag agccaggcac tgccccagga gacagggaga ggagacagac
38101 ccagatgccg ctgcaggaga cagggagagg ggacgaagcc aggtgccgcc actggggctg
38161 agcatccctg gttagaagac gtgggttctg gcagaagttc ctatgttttc acaaagggca
38221 agggtttact ggccggtttg gggctgtcct gaggcggggt ggagKtctga ggccagggga 38281 cacaaggtgg acaagtgaag tggcctagtg gaggcagatg ggggtggcag aggggacggg 38341 ggcggcaggc gggtggggag cgtgggtggg cgggcacact gcatggtctg cccttggctt 38401 ctttccttgg ctcttggaga acatggggtg tggtcaaaaa attgggtggg aacagtgcaa 38461 tcccagcgag taatttgccc atttcattcc Yggaaaaagg agaagagaaa cagccctgta 38521 tggctacact atcagagctg aatatggcac tgaaaagctt ccatgatggt tgataagact 38581 atgggaggaa ggaagcctgg tttgggtgca gcctgaccag tggcgataat aatgatgcca 38641 tctgctaaat atgaagataa ggcaagggga caggcgtgaa ctggagaggg tctgcgggca 38701 ctgcccggct gagctgtgat gctgctggga gcgctcagac tcctcttcag acccggaagg 38761 gctgcggagc ttgttgggac agatgagcag gctgggcggt gcactgggca ctgctgtcct 38821 gatagaccct gcagtctccc aggacagtgg tgYggtggcc tccgactgtg acccttggca 38881 tcccaccatg catgtctgac cccagatttc aacctctccc acctgccctc catgtctcct 38941 tctctctgaa ggtttggccg taagctgtgt ctcctgggaa ctgtgctggt caacgcggtg 39001 tcgggcgtgc tcatggcctt ctcgcccaac tacatgtcca tgctgctctt ccgcctgctg 39061 cagggcctgg tcagcaaggg caactggatg gctggctaca ccctaagtaa ttatcatggg 39121 gatgaggcca ggtctcaggg tagaatggga tgggcctaac gtgggtaggg ggttgtttgt 39181 ggggtcaagg agcttgcggg gcaccagttc cttgtattta tgtgactagg gcaggacatg 39241 ggctagaatg gcctcctctc tttttgtgtc tgagacactt aaattatcac ttagattact 39301 gacctattaa tcaactaatc tatttgggga acaaaaagac cccatgctga tgtgctcaca 39361 gcagtcagga tggggtgcag aagaggagat aagtgagaga atattctagc attttacata 39421 agaaagaacc agcaactcct taaaataaat acagcaaact tgacgggctt gaatggcact 39481 caagaattag gaaagaaaac aggagcaaat aaaataagga ttgtgaaaat caggattttg 39541 aaaaagttga agtataaacc aacatgagga gttttacata tcagcctttt tttccctttc 39601 aaatattcta aggttaagaa tacaaaacaa aaaacccttt ccgggaggaa tgcagcctaa 39661 gagtcatctg tacttcccca ggccagggca cggcagccag cagtgaggat gagccacact 39721 tttactcatt tgtgggggag aaaaaagaaa tagcctgtct tttcatcttt gaaaacaagg 39781 tgctatcact tggcattctg agaactcatt cactagggcc aagaggatcc acgttcccgc 39841 cttgagaagc catgagcagc ctggtgaagc tgagctggct cagccccatg cgtgtggagc 39901 ctgtgacagc ctctgctgct aggcaccgtc ccacacatgg ttWggggggt ctgacccggg 39961 gccacatcca cgctccaaca ccacccgcag cccctccacc cacgactccc ctgacttcct 40021 tttccccttt tcatttttgt tcccgttaca cgtatcacgc tctaacatag gattgcattc 40081 tcttatctgt gatgtggagc gtctgccaaa tgaggtctcc cagggcagga ctctacctcc 40141 tatgcttaac gatgtgcctc agacaccccg cacagtacca ggtccccagg aggcactcaa 40201 agatatttgc taaatgaatg aacacccctg gggtcctgac aaatattcca gctgagtctg 40261 gagcttctcg agRaaggcgt tggggaggag aggccagtgg actgagtcct gaagcaggca 40321 gaggggagtg cagatggtaa aggagcagaY gcggaaagcg acggtcaggg aaaagccaga 40381 gagccaagca gcctggtgga tctccggggc cYYggtttga tgggtccggg gccccggttt 40441 gatgggtccg gggccccaga gagaagggga ggcgtgagcg cggctgtggt ttgcgggaag 40501 gagaaatggg agacacacaa gagagaagcc tgggagcagg tgaggctggg caaggccggg 40561 tgggagcttc ccgcggggag acaagctgga ctggggcccc gagcttctga acgcacggcg 40621 tcgggctcct gggctcctgc aaggaacccg cataacgtcc acacctcctg tttcagtcac 40681 agaatttgtt ggctcgggct ccagaagaac ggtggcgatc atgtaccaga tggccttcac 40741 ggtggggctg gtggcgctta ccgggctggc ctacgccctg cctcactggc gctggctgca 40801 gctggcagtc tccctgccca ccttcctctt cctgctctac tactggtgag gcccttcctc 40861 ctgcctacgg ggaaggggcg ccggccaccc ctctggctgc gctttctgtc ctctcgaggg 40921 accagtctct tggagtgggg ggcgcctaca ggccgtcttc caaaggccat ggtgtccaca 40981 tgcataacgc atggggctgc gcggagcccg gcgcttccca cactcatgac gcatagggat 41041 gagctggtcc cggggtttcc cacacgcatg acgcataggg atgagctggt cccggggttt 41101 ccgacacgca tgacgcatag ggatgagctg gtcccggggt ttcccacacg catgacgcat 41161 agggatgagc tggtcccggg gtttcccaca cgcatgatgc atagggatga ggggagcctg 41221 gggcttccca catgcatgat gcatagggat gagcagagcc cggggtttcc cacatgcatg 41281 atgcataggg atgagcagag cccggggttt cccacacgca tgatgcatag ggctgcacag 41341 agcccggggt ttcccacagg catgatgcat agggctgcac agagcctggg gtttcccaca 41401 cgcatatcgc atggggctgc gtggagccag cgcttcccac cRcttgtact gttcaggttg 41461 ttctgttatt accaggcggc ccaggttaca tgtggagctt tcctcagaca aagcctcttt 41521 agataggttc ccccagataa aggtgggcca tgccaagagt cggacagaaa tccaggaaaa 41581 ctcagggcct cagcatgtgc actttccaga aacacaaggc tgaacctcac tcccgggtga 41641 aggaggcagc tttttgaggg ttgctcagtg gccttgggaa atctgtatca gcgtccagtg 41701 gtaggaaagK ctccacaggt ggcaatccca Stgcaatggc taccactccc gagagtcccc 41761 tagagtccgt cccaggggtg ctggcatgaa gtcagatgct ctgttatctt cttgatcctc 41821 tccctcttcc ccctYttctc ttcctctctg ctgtcatctc tgctcttctc ccRcttcttc 41881 atttKtatag tactattggt attattatta agacactgaa atacctctca tctagccata 41941 gagcaggcat ttgatcttga ttgaatgaat gagtgacaga atgaaggaac aacaggcctg 42001 ctcaatgcaa ccttctctgg ggactgtgta ccctgagcca ttatgacaca gaatcagtcc 42061 aaacaaggtg ttaatcatat tagctgtgag tgcttcgcag agtctgaagg gcaaagaact 42121 ccacaggtca gtttttattg tggtggaggg aagaggRggt agcagaaagg tccacagaga 42181 ggaatttctc atccctccac cttttgtctt agttttgctg agagcagacc tcatctgtgg 42241 gtggcactag agttaaccca gtctctctaa atgaaagagt tgtacacgta tggccaggga
42301 gttctgagga gcctgccctt cccctgcctY gtccttgtga aacagggatc tctccagcag
42361 aaaggcaaat tctgcatgct caggcagggt gaggcagtca atgccggcca gctttcagaa
42421 gacccttatg ccagagaggc ccagtctttg agttgtaccc tcctcgtccc atttgaaata
42481 gacagcatgg tgttgatttg attttaaagg acagtaaata cattttattt tgtttgagaa
42541 atattttgag aagtgttcaa cagcccttgt attgcctgga gatgtcctgg tgtcatcggg
42601 taatatacat ggagcatctg ctctgtgagt gttttccgtg ggggacaaag gctgccccag
42661 gagagatgag gcccctgctg cacagaagga aggctacata ggaagcgatg gctccctttt
42721 ggtctataaa tcttaggtga cactaggagt ctgagtgacc cacagagctg tgacctgggc
42781 cacatgggct agcgcacagt gaccccaggt cctcatcctc ttgagggatt acagccccaa
42841 cgtggggagg gcaggctgca ctgagcaaca gcatcacccc gctcagggct gaacgtcaga
42901 ccgaggaaaa tgccagatag tgatgagtgg tgttcgcagg tgtgtgccgg agtcccctcg
42961 gtggctgtta tcacaaaaaa gaaacactga agcaataaag ataatggacc acatcgctca
43021 aaagaatggg aagttgcctc ctgctgattt aaaggtgaaa tctaggaagg ggtatctcac
43081 atcactgaat ctggggctgg gttctggtgc agattcgtct ggggctgcca ggggaaagag
43141 cagaactgtg ccccttgtca tgggtgtgaa gcacggtggt ggcaaggctc acctccccag
43201 ggRctcccag gtggctctgc tcatgacagc gtgagtgtgg ctgatgctct ccctccctcc
43261 tagatgcttt ccctcgaaga ggatgtcacc gaaaagctga gcccttcatt tgcagacctg
43321 ttccgcacgc Ygcgcctgag gaagcgcacc ttcatcctga tgtacctgtg gtgaggggcg
43381 ttcctgtgcg tctctccagg cagaatcagc acgctcgccc aagcactggg ctatagatta
43441 gagacagtgg aatactcagg tggaaaaaga gaaagggaaa aagaattgta atatagttgt
43501 gagtggtcaa agagctcttt ttgtttctcc cttggggatt gaataataag gaaaaggctt
43561 cattttagtg aggagcattt gttacctttg ggagatcacc agtgggatga gagaaatagg
43621 ataaagggtt atcggggctg cctccgttcc acatggtctc atcttctggg aatagccaag
43681 tcttgactcg gcgtcagaag gtggatgaga gctcctggag cgttcgagga ggaagttcca
43741 ttcctcatat ctaaacaccc tagagaccct acctgagcat ctctgtgcat ttgttaccta
43801 ttgactagag aacgcacacc cacatggacc cacgcacaca cgctttgcac attccagggg
43861 tctgcatgct cccccagctg ctgtcacgag caggaatccc ctgccctgct ccctaggagg
43921 atgctggctc acctcctccg cacaagggct ttcccctcag catacagtga gggcccccca
43981 actgcactct gggcctggcc ccttcagcgg gagcagctcc gctacccagg cacacttgtt
44041 tgctcttcta tgtgaggttt tattggaaca aagagtttgt agtaagtcag tgcctggcat
44101 attgcagacc ctaaatcaat atttgctgaa agttgaatga gtaaacactg tgccccagaa
44161 tctgtccagg ttcagtgtgt cctgtcctgc ctcaaccttc accctccctg tctccgagtt
44221 tgccgctggg gtcctgcacc cagccacatg ctgggcccgt gaacctttgc aaatagcccc
44281 atgtacctgg tgggtttcca tggatactca gtgttgaact cagcatggag gctgggactg
44341 gccgctgcag tggaagtaac tgcccgctct gctacgctgt tcagctcccc tccccagctc
44401 cgagactccc cacaaggcag tgttcttgac tcttctgttc tgttaccccc acatccagtc
44461 ctctggcaag ttccatcaac tggcttggaa atgccttcag aacctgctgc ctcccatctt
44521 gggcaccctg ggttcccttg ctggggttgt agtagccctg tccttggcac taattctttg
44581 tataaatgtc agctgtctca tggacagttc tgcagcatct cttacctgca ttactgtagg
44641 caccttctgg gtggcctgct ggctttaccc atgcccctcg gtgctccaca aagcaggcaa
44701 aggcatccct tagagctcaa cagaccttgt ctctccctgt ttaccctctg ctggcttcct
44761 aatcactgag aataaagcca gatccttttc tgtggcccac aaggcctcac gcctttggcc
44821 cctgaacgcc cttttctact gtcctgctgg ttgtcatgac tgagactcga aaggtctgtc
44881 caccatgtcc tggctgtgtg accttccaaa gggccttaat cKctccgtga ctcaatttcc
44941 ccctcagtaa tgggagaagt gatagtacct atctcacaga tgtgttggga ggattaagga
45001 gattatatgt gtaaatgaca tagaagaggg tacagcatcc atgcaggaaa tgttcaatgt
45061 caatgtggtt ttttttgtta ttttaataca cacatgcaat tcatgagctc tgcccacagg
45121 tggcagcccg gcacatttgt taactggctt gttgacagga aaatcaaggt tacacgttcc
45181 tttctcttaa accagtttct ttatcagtaa tatgactgct gtctttaact ggctaaatgg
45241 tacagaggca ctcaggctaa cctcaactcc ttgagttgca ggcaagcacc ttgtttcttg
45301 ttctcttgac cctttgtcca atcaggaata atttaggact agaaacaaaa gcatcccaat
45361 gcttctttag gcgaaatttt atcaaagaag gatacattcc ttagcaacat gcattttcat
45421 ctcagttttg gagctgatag gcaatctgca tgctgggatg agacagtaat ttcttattga
45481 cccaaatctg ttctMacaat gtaaatatga ctgtaaaagc tttctagctt ccaaatgata
45541 ttttcccccc gaagtgacac atgaggcacc ctacattttg gctcaggtga gagtgattta
45601 gctaggcctc gggtctgtgt gccatatgct gtaatgttgc tacattttct caaatatgga
45661 cggagggggt gtgcagtgaa tggaagagcg taggtgaatc aatagcgccc actaaggaca
45721 gcacagtaaa gggccgggaa tgccatggtg cggttggaaa gacctggccc atgcctctgt
45781 ctgttgtgcc ctgagtcttc tgagtgccct gagtgcatac tgtgtgctgg gaactataat
45841 tggcactttt gtacgctaat tcagtgagta ctcaagacaa acagatgtgg gaggtactat
45901 tacaatccca tttacagacg agggaactga ggcccagaga ggttagttaa cttcctcaag
45961 gtgacacagc tcataaagca atccgacacc tggcccctta cttcctggtc tctcctgccc
46021 acctcctcgt aggcctgttg gggtgtcaga tgaggtacgc acgtgcaggg cttggtgcag
46081 aaccagacat ttaaatctgc agtgaacagt agccatcctc acgtgcagtc tccaggagac
46141 ttggaggcat atcctacaag tcagactctc acatctatca tgaaaaaaat gtatcattgg 46201 ggcccttcta ggacactctt tctcattttt actcccctct tctcatattg ctctagggca 46261 ttctaaaccc agtgattcat gctctttctc catctgcgag gggctttttt tttttttttt 46321 tttcttcagt ctctgactca tgcctttgac ttgaaacctc ctcttggctc aggttcacgg 46381 actctgtgct ctatcagggg ctcatcctgc acatgggcgc caccagcggg aacctctacc 46441 tggatttcct ttactccgct ctggtcgaaa tcccgggggc cttcatagcc ctcatcacca 46501 ttgaccgcgt gggccgcatc taccccatgg ccRtgtcaaa tttgttggcg ggggcagcct 46561 gcctcgtcat gatttttatc tcacctggta agttggtaag ttgtctgctt tcatcatttc 46621 ccaggcaatc gaagtgtggg gaaagtgttg tgactctttg ataagcctct ggaatgagaa 46681 caaagatgag gtgctacctt tgtctacaaa gtttttttag attttaaatt gaggggccgt 46741 gcacagtggc tcatgcctgt aatcacagta ctttgagaag ccaaggcagg tggatcacct 46801 gaggtcagga gttcaagacc agcctggcca acatggtgaa accccatctc tactaaaaat 46861 acaaaattag ccgggcttgg tggcgggcgc ctgtaatccc agctacttgg gaggctgctt 46921 gaacccagga ggcggaggtt gcagtgagcc gagatcgcac actccagctt gggagacaga 46981 gtacaactcc atctcccaaa aaacaaacaa acaaacaaac aaaaaaaccc ccaaaaacag 47041 caaaaaaaaa aaaaattaaa ttgagtttta acatacatac aatcagcaaa ctataagtgc 47101 atgactccat gagtttttaa aaatgtgtac acccgggcag catgatccac gtaaagatgg 47161 ggaacctttc caacttctcc aaagcctccc tcaggctcct tcacaacaac ccctgccacc 47221 cctccaaggt caccactact ctgccctttg tcagcacaga ttgtgcctgt tcttgagctt 47281 catatagatg gcaccatacc aaatatactg tttttgttat tgttgttgtg ttttgagaca 47341 gaatcttgct ctgttattca ggctggagtt cagtggtgca atcacagctc actgcaacct 47401 tgaactcttg ggctcaaggg atcctcccgc ctcagcctcc tcagtagctg ggactacagg 47461 cgtgagccac tatgcccggt taatgttttt attttttgta gagatggggt cttactatgt 47521 tgcccaagct ggtctcaaac tcctgggctc aagcaatcct cccacctcag cctttcaaag 47581 tgttgggatt ataggcatga gccattcatc attgcaccta tgagacccac ccatgttgtc 47641 acatgagagt gtagttggta cttcaaacaa aattattgtg taggattcca ctgtatgaat 47701 atgacactct gcatcccttc cactgttgat tgtcacttgt gttgcttcca atgtttgcca 47761 cttattggga ctaaaatcat ccttgcacgt atctttgggc aggcacatgc tttcatttcc 47821 cttgggtaca ggtctgtgag tacacttgct gggtcccagg gaggcatatg tttagctcta 47881 gcagacactg ccaaacagct ttccaaagtg gttagaccat tttatactcc tcccaggaac 47941 acatgggagc tccgattact ccagatcctc ccaacacttg atctggctca tctttttaag 48001 agtggccatt ttggtggatg tatactttag tctgtaattc ttacaatcaa agataaacca 48061 agctacattt aaacctaaag cagattaact tcagtcagtt ttaatttgag tacgtaaatt 48121 gcctttcaca ttcattgctc ttggatatgt tgtaaaataa atacaagggc agatctgctc 48181 ttttctcagt ttttactgcg tgtgctctgc acttgctgat ttagtgagtc attttggcag 48241 gctttcgtgc agggtattgt agagactgtt ctgtgaaatc tgtatgccca agaactaggc 48301 tgtgtagatc aggctggaaa gaaaaagggt tggggaaaga aagctcagtt ttcttttccc 48361 tttttttcat ggagaaagaa cagagaacac aggaaaaggg gcagcatgga tctgaaatag 48421 attttgctca ctttcttccc aatctctctg tttctggaat cctgggtaag atgtgatgga 48481 tgggcatact tttaaaaagt tcacagcact gagtgccaag aaggagggcc atcgagggct 48541 aagtccattc agttgtcacc tggacagagc ccaggcatcg tcccctcccc agccccctcc 48601 tcccagctcc tgtcctcccc tcgtccctct gtcttagtcc ttatcacatt ttaggacagt 48661 cattcattca acccgcagga aatatctcca ggccttctat atggctggca ggtatctagg 48721 tcctaggatt tcaaccatga gcgaagcaga gagagttcct gctccatgga gctgacattc 48781 cagagggaaa agagaaggat gtcagtgctt tctacaatgg gcacgggagg cctgaagagg 48841 cctaaatgaa gtgggaagag ccatgtaaaa atctggggag agttttctga caYgtgcaag 48901 gcccttgggg tgagaagaat gtggcattgg agggactgct cgggatctag ggacaagggt 48961 caggcgagct cgtggggagc agtggagtga ggcgtggagt gagttgggaa gtagccagct 49021 gtggacttta tcccaagagt ggtaggaaac cactggtggc tttgagcagg ggtggacttg 49081 acccaactcc agtctgaact gggtccctct ggctgctgtg atgagcggcc ctgggggtgc 49141 agagtggagg catgttcagt tctctgaggc cagggatgct gtttacctct ggtcaagcat 49201 caccaggcta aagacctgtg aatgaggacc tccctcgagg cattggagtg aactccttgc 49261 cttttatctt ttagaagcta gatgaggtgt ggtccttaac agaaggctca gcttggcttc 49321 tctatcaggg ctgagagctc caggaggcag agagactgtg ttgcacccat gagacgggtg 49381 aggggcactc tcatttggac cctcacactg gctgagtgag atgatacaag atttccataa 49441 atagccagta tctggggaca agggtcaagc tgacatcgtg tctgtgaccc cagaggcctt 49501 tatagtgagc tggtgtgtca cagggttact tgcatttcag tgctgggggt aacccatgca 49561 gagcagatga tggtggttat taaggatctg agcttctttc caatgaagcc aaattctttg 49621 atcattctca atagggagag ggctttctgg gccttcaggt ggtcgagaac cccacagcag 49681 cctgccagcg tgaaacaagg gctcgcttcc ctcattctca gcctctgaga ctattatgaa 49741 aggtgttttg gttaaaactg tattggaaac aaatattaaa ttgaggatta gaaatctgtt 49801 ttgttagttt gagaactact actagaggga ttatgtttat tgagtgcttt ctgtgtttca 49861 ggcactgaac tagaatggat actatcatat aataaataaa aagccaactt taaaattctg 49921 tggtcacagt ggacatttga catacatcag ctctaagtct catgatgatc tataagctgg 49981 gtgtcattat tcccatttta cagcccagga aaccaagctg agattcaaaa agcccattct 50041 ttcccctgtt caatggagtc ttcttgaata tgtcatcgtc aactccccaa atctcttgag 50101 actcagttta cttatctaaa aatgaaaaag ttagataaga caaacttcca ggccccacca 50161 gctStaatag tccaagcatg acccaccatg gcctctcaca gtaactcagg gaacgaagcc 50221 cccatccacc acccacaccc tctttgtact tcctttccag acctgcactg gttaaacatc 50281 ataatcatgt gtgttggccg aatgggaatc accattgcaa tacaaatgat ctgcctggtg 50341 aatgctgagc tgtaccccac attcgtcagg tgagtgcatg gaacaggggt agccagtgaa 50401 atggctgtga acccaccagc tcagtgggaa ccaacatctc caaggtgctt cctaaagatg 50461 cagccaagca ttgctcagtt tggacattga gtggcattgt taagaaatca cgttcagaga 50521 gatggggtta agcatcaaga gagggtcaac ccataattat agcagagaat gtgaaagggg 50581 accctgtgag ctgatggatg gggtcaaagt tgttctgtac atacaacaag tacaagaaca 50641 aaaggtgtag agaagtgggt actggacagg gacttgggaa acctgtgttg tagccctgtc 50701 cttggcacta attgattgta taaacatcag ccatttcatt gacacttctg ggcatcattt 50761 tcttaaacag ctgacatttg tatagtgata cgcgagtatt tccatacatg tgattccctt 50821 aaatttgttc ttgggtatga tcatccccat attacatatg aggaagctgt gatttagaaa 50881 gggaaagaag cgttgtaggg gtcagacagc tagaaagaag cagggctggg gcaaggacca 50941 catcttcctg acctggaacc ctgtggtttt tccattatgg cacattactg ttctgtaaac 51001 tgaggaactt ttcaaaattg tggtaaaata tacataatat gaaatttact atcttagcca 51061 cttttaagtg tacagttgag tagtgttaag taccttcaca caaccaagct ccagaactct 51121 tcatcttgca aaacggaaac tctgtactca ttaaacagta actccccata ctctccaccc 51181 tccagctcct gacaaccgcc tgtctacttt ctgtttctat gagtttggtt actttaggta 51241 cctcatataa gtggaatcat atagtatttg tctttgtatg actggcttag atcacttagt 51301 gtgatggcct caaggttcat gtatgttgta gcatgtgcca aaattgtctt ctttttaaag 51361 gctgagtaat attccattgt acatatatac cacattttgt ttatccattc atctgtcaat 51421 ggacacttgg gatgctccac ttcttggcta ttgtgaataa tactgctatg gacatgaatg 51481 tacaaatatc tcttcaagat atctttcaat tctttggggt ggaatatcca aaagtggaat 51541 tactggatca taatctaatt ctattattag ttttttgaag gaacggctgt attttttttc 51601 cataacggct acactatttt acattcccac caacagttca caaaggttcc aattcctcca 51661 catccttgcc aacacttgtt aatttctgtt Kgtctttttg atagtagcca tcctaatggg 51721 tttgaagtgt tatctcattg tggttttgat ttctctgatg attaattatg ttgatcatct 51781 tttcatgtgc ttgttggcta tttgtgtatt ttctttggag aaatgtctac tcaagtcctt 51841 tccccatttt tgaactggtt ttgttgttct tgagttgtag caatggttta tataatctgg 51901 agattaactc tttatcaggt acaaaatctg caaatatttc agcccattcc ataggttacc 51961 ttttcattct gttgattgtg acctttgatg cacagaagtt tttgattgtg atgtagtcta 52021 atttatctct ttttactttt gttgcctttt cttttggtgc tgaagaactt tttttttttt 52081 aatcaagttt attgaggtac aattgacaga gtaaaattca ctgcttctaa catgcagtta 52141 gattagtttg acgacacaca cacacacata cactcacaca cacgtatata tagtcattta 52201 gccactgcca tgatgaaaat agagaatatt tccatcaccc ccaaaaggtt cttcatgtct 52261 ctttgcagtt aatacccttt tcttctcacc tccagaccat gcagttttag cttttccaga 52321 atgagctgta aatgtaataa tagggcacgt caccttttgt gtttggcttc cttcacttag 52381 catgatgaaa ctcacccact tgttggatgt atcagtgttt tgcttgttgt tgctgtatag 52441 tactccagca tgtgaatgtc agtgtgccac aattcataca ctgtttagct attcaccact 52501 tgagggacat ttgaacggct tccttcagag ggattctgat gcagatgcta tagacatttg 52561 ggtacaggcc tttgagtgga tacatgtttt catttacgtt gggttaaaaa ctcaggagtg 52621 ggattgttag gtcatatggt aagtgtgtgt ttaattctat gagaaactgt cagactgtca 52681 gaccgttttc cgaagtggct gtgccatctt gcgttcccac cagccatgtt tgaggaatcc 52741 tgtcgttcca cattctcatc agtgtttggt gttgtcagct tttttttttt ttgagatgga 52801 gtttcactct tgttgcccag gctggagtgc agtggcgtga tctcggctca ctgcaacctc 52861 tgcctcccag gttcaaacga ttctcttgac tcagcctttt gagtagctgg gactacaggc 52921 gtgcaccact gcgcctggct aattttttgt acttttagta gagacagggt ttcaccatgt 52981 tggccaggct ggtcttgaac tcctgacctc agatgatctg ccctccttgg cctcccaaaa 53041 tgttgggatt acaggtgtga gccaccRcac ctggtctggt gtcagctttc taaaagtcat 53101 tttggtaggt aatggtatta ttgtgacttg acttttccta attactgata atgttgagta 53161 tctttgtctg tacttaattg ccttctgtat cttggaagac ttagttttaa atgaagatta 53221 ttttaattgt aaaagtaaac actttactta aagttctaag aaattctagt ttgttagaaa 53281 ttccgtatgt tacttaaact ctttttcctc tgtatacatt gtttcttctc cttatgtcat 53341 cataccagta gtttctagga aataccccag ctccctaatc tcatcttata catgcgcagt 53401 gctctgagcc cttcagtaaa gccagaaggc accaggatgg ccttgattca ctctttcctg 53461 actaatcctt tattaacagg ccatgtctga gactctcatt ggtaaaaagt tactgggact 53521 gagtacagga gatgaaggca tattaaaaag aaaaccttct atatttggca gttctgagcc 53581 cagagtctaa aatagccagg ccaaacaatt ccattKtcat ggccactggg ccaaggggac 53641 tgactcaaaa tgtaaactgt gaaaggtact gagtgtgtag ttctctaggg agaaaggcca 53701 agcaacccat ccccgaggag ccctcgacca ctgctcagag agctggtggg agaatcattc 53761 tcattcacta gggagggaag ctagatgttc agaaaaaact ctcaacttct tatgtacaca 53821 cgcgcacaca cacaaggtaa ggcagtcatt caagttccaa tatttctaat caattgaatc 53881 aaatttaatt tgcagagaag gaataatttc tgtgacttta ttactttata agcactccaa 53941 gtttagacag tctctatgta aaccattctg accctgcctc cgttccaggc agtttcagag 54001 ttggactgct ctgctcctct tcactttgtg cagcacgtgt tgtaagaaca ttcatttggc 54061 attaataccc actgccttgt tttgaaaatg atctttccat ttttatctcc tcaccttctc 54121 atgagctcct tgaaaacaga aaccacatgt catgtcatac ttctctgtac ctcacagaac 54181 tcttccacag ccagactcac tacatgctgt agaatgactc aatgaatgaa gtaccgcaaa 54241 catctgaatt ttaaataagg ggcctagttc cctacttctt ctagcttctg tgtgcttgcc 54301 atttttttcc ttttaacaat aattttttaa aaatagtaaa aaataactgt gtcttttctt 54361 gcacctcttg gaaatcacac aaaagctatt agaagaatga gggataatgc aaatgtgttt 54421 gaaactgtga taacatgaaa agacaatcta caagaggaag ggctgcctag tgtgtgaggt 54481 ctaagggagt ccgagaagat tccaggaaag gacccctgtg acagcaaatc gtaggcgagg 54541 ccagcagctt tccaaagtag acagcgtgac ttgaagggtg tgatccatgg tcccttgttt 54601 ggagaaacag aatgatgggt gcatgggctg gaggtggaag ctcccttccc tggatgttgt 54661 atggctgaag gtgaagcagg gaggRcaagg ctgtaaaaga agatatgcag gaactttcag 54721 acagcagtta ctgagaggcg ggtggaagag actagctcca caccatcagc ccctgtagat 54781 caggaataag tgagccaaaa aaatctaatc atatacaacc ctgatcaaag aaagaatttc 54841 tactagccca gaacatggcc tggctgcttc cccacaaacc cttctagaaa tggctgatag 54901 aggaagaaat aacagtatga aaagatgagt aataaacaaa aaagtaattc attcaggagg 54961 tacacaaaca tatcatgaga taaaggggga aaggagcaga aaaagcaaaa ggcaaatgaa 55021 gaacatgtac cagaaaaatg ctgtaacaac atagatcaaa gttctaacta catatactac 55081 caaaatgtaa agtattaata aatcagtcat ctctataaaa ataagacttt aaagcagagg 55141 tatagttcta gagagaatat ctggcaagac aatagaagac actgaagctg tcttaagaaa 55201 gaaacgaaaa agaaaaatta agaagtggag aaattcttgc atttgagaaa tgaaaaacca 55261 atttgaacac aatgcaaagg agaatagatt cagtgaggga aaggcaagac aggactaaga 55321 gttgcaaagt aaaatggaaa taagaaataa gcagagttta atccaggcat ggtggctaac 55381 gcctgtaatc cctcactttg ggaggctgag gtgggcagat cacttgaagc caggagttcg 55441 agaccagcct ggccaacatg gtgaaaccct gtctctacta aaattacaaa aattaactgg 55501 gcatggtagt gcatgcctat agtccaagct actcagcagg gtgaggcagg agaatcactt 55561 gaacccagga ggtggaggtt gcagtgagac aagatcgagc cactgcactc cagcccgggc 55621 agggagactt tgtctcaaaa aagaagaaga aaaaaaaaaa aaaagaaata agaaataagt 55681 agagtttcaa aggttctatg catgtatgta tgtatatagt ccgtaaactt tttggttaaa 55741 aaaaaaaaag atatatatat atacaccccc tgcccacaca catacgcaat aaatatactt 55801 cgggaataca tctgaaaata aaagaatgct tgaatctata aattaaaggg acacatgtaa 55861 cagaaaaaac tgacccagtg aacaattatt aggacatatt tttatttggt tactatactt 55921 taaagataga gaaagagtca ccaagtagcc aggctgaaag tttgtcacct atataagaag 55981 aacaaattgt tatcatttga tgaagacagt caatgcctgc agtgacctca agaaaataaa 56041 gtgcaatcca gtgattttta ttattgtttt tttgagacag gctggagtgc agtggcatga 56101 tctcggctca ctgcaacccc tgcctcccca cttcaagtga ttctcctgcc tcagcctcct 56161 gagtagctgg gattacaggc gtccaccacc acgcccagct aattttggta tttttaggag 56221 agatggggtt ggtcaagctg ttctcgaact cctgacctta gaagatctgc ctgcctctga 56281 ctcccaaagt gctaggatta cagatgttag ccatcacgcg cgaatccagt gaattttatc 56341 caaccaagct ctcatttcta tatagaagca atagccaatc atttaaaaat atctcaaata 56401 atatggttcc tatgatcctt tcccaaaaaa gttgttagaa acaaaattca gtaagtacag 56461 tgacgaatga agtaatgtgt cagaattatt ggtagtgatc attgaaattg atattaaaac 56521 gagtgtgttt ttggctacaa aacaatataa tatcacaaat ctgaaaataa agaaatgtta 56581 taactcacaa agactgaaaa gagaaaagag aaagaatgtt ggagatggtg taatacactg 56641 atttcttcaa tacacttgtt ttttggaaaa gtgtatcctt aaaaactgac aagtataatt 56701 agttcagcag caatatattt ttaaaaactg ataagtagta tcctaggcat atgttaagat 56761 ataaaagaaa acactaaaag taatgataga atcaactaaa tcaggtcatg gagggatggt 56821 ttgaggggga agtagaaata tactaatttt atgattgttc ataataggaa gttaatcaat 56881 actgtctaaa gaaataggat attaattata taaggttata cagtaatcat ttgactaaaa 56941 atacagccct tccaaatgat caggaaaaat acagataacc aacacatact gaaaataaaa 57001 gacaccagaa gcaataaaaa gccctggtgt gaataggtga tagaatatga atagatgaca 57061 gcattgatat gacagaactg acatggtaga attaagacga aacatttccg acatttaaat 57121 aaatgaaaag aggcttaact cctttattgg aaaaaatgag atgactccct agcttgatta 57181 caaagtaaaa tccaacaata tgctgctata tgaagtataa cttttcctga gaaaagctaa 57241 aaaagaaaga atgcacaaaa gcatactagg gaaatatata gcaagtgtca taatctaatt 57301 aaagttgaat ttatgggaaa aggtgctaaa taagataagg agggccctaa ataatgataa 57361 agaaggaatc cacaatggtt ttaaaggggg atttttaaaa acatttaaca aatagaaaat 57421 tccaataata tttaaattgc tctaaagcat agaaaaagaa gaaaattttc caactttaaa 57481 aaataaaatt agcgtaatat tggtaccaaa gaacaacaaa gataaaggaa acaaggaaac 57541 tacagacata tatttatgaa tatggatcta gaaatcctaa acaatatatt atcaaacagt 57601 ggagaacatt aaaataatta ctattctatg tcttagatga attattccag gagtgcaaag 57661 atggctgagt tttagaaaac ctcacagtat aaatcatgtt tattagtcaa aagagaaaaa 57721 ttatattatt atctccattg atactgaaag gcattcaata aaatttccac ctctacgttt 57781 gttttaaaaa tttctaagag atgatataag catttttatc atcaggatat aggtcatggg 57841 gggagggatt gggaaagaaa tataaatata tgaattttgt aattagtcat agtagggagt 57901 aagaaattat gcaaataatt gcaatagaag aagcttaaaa gtaatacccg aaaggataga 57961 acttttYctg atttattatt cctattttaa tcatgttttc aaaacaaagc tcaagattgt 58021 ttctaggaat acaaaatcga gcatggaaca aagtgaaatt tacaatttct gacatccaat 58081 aaaaaattac caggcataca aagaagtagg aaaagataac ctatcttgag gagaaaaata
58141 aatcaattca aactgaccta gaaatggcac agatggtaga attagtggac atgaacatta
58201 gaacagttat gatacctata ttctctatgt ttaaggtcct agagtaaaga ttgagcatgt
58261 taagtagatg caaggaaaaa aaatttaagt cccaaattaa acttctagag ataaaaacac
58321 aatgtctgag atgaaaaata caccacatga gttaacagca gattaaacat tatggaagaa
58381 aatattagtg aacttgaaga tagcaattga aactatcaaa aaggaaagac agagagaatc
58441 agtgagctgt gatacaattc aatgaatgta attggagtct gtagaagtga gtaggagggg
58501 ttaatagaga gagaaataat gctcaaaatt tcttcaaatt tgatgaaaac ttcMaataca
58561 tagatctaac aatctcaata aatactaacc acaaaaaaac atcaagaaag gcacatcata
58621 aaaatcttaa aaagaccagc ctgggcaaca tggtgaaacc ttctttctac aaaatataca
58681 aaaattagct gggtgtggtg gtgcatgcat gtagtctcag ctactataga ggctgaggta
58741 ggaggcttgc ttgagcccag gaggtggagg ttgcaatgag ccaaggtcac gccactgcac
58801 tccatcctgg gcaacagggc aagaccctgt ctcaaaacaa aaacaaaaac aaaacaaaaa
58861 ccttaaaaag gaatcagaga aaaaagatat cacaaatagg aacaaagata agaatgacag
58921 aaaatttctc attggaaatg atacaaacaa gaaggtggta gaacatcttt aaagtactgM
58981 aagaaagaaa aataaactgc caatctaaaa ttcttgaccc agcaaaaata ctttcaaaaa
59041 tgaatggaaa ataaagactt tttcaaactt acaaagtctg aaagaattaa tcacYagcag
59101 acttatacta caggaaatat tagtcttgaa gatctttcag aaaaaatgaa aacagtatca
59161 gatggaaatc tatatctaca caaaaaaatg aagagcactg aaaatgataa ctacgtgagt
59221 aaatggaaat tttttttttc tggttatttt ctttaaaaga caatgaactg tttaaagcag
59281 gcattggcaa acttttccag tacagagctg gatagctaat gttttaggct ttgtggatca
59341 catacagtct ctgttgcata ttattctttc tttctttctt tctttctttc tttctttctt
59401 tctttctttc tttctttctt tctttctttc tttttttgaa atggagtctc gctctgtcac
59461 ccaggcggga gtgcagtggt gcgatcttgg cttactgcaa gctctacctc ccaggttcac
59521 accattctcc tgcctcagcc tcccaagtag ctgggactac aggcacctgc caccacgcct
59581 ggctaatttt atttatttat ttattttgta tatttttagt agagaaaggg tttcaccatg
59641 ttagccagga tggtcttgat ctcctgacct cgtgatccac cctccttggc ctcccaaagt
59701 gctgggatta cagccgtgag tcaccgcgcc tggcctaaaa aaccttttaa tcatgtaaaa
59761 accatttcta actcaaagct aaaaatgctg tctttggcct gtgggctgta gtttgccaat
59821 caaaacaata atcacaatgt gttgtgcaaa ggctgggagg gaagaaacac aagtatacta
59881 ttgtaaggtt cacatataat ctatgaatgt tacaatatta cttgaagtta ctgtgatcaa
59941 agagttaggt tttctgtaaa cctaaatcaa aaattaaaaa acaaaataat tatagctatt
60001 aagtcaacac agaagataat gtgaaatcat ttaaaataat ttataagaag gcagaaaaag
60061 aaataaaggg ttacagagga caaacagaac aaaaagaaaa caaatagcaa gatgatagat
60121 ttaaactcaa tcatatcagt aatcacaaga aatataaatg gtctagacac tccagttaaa
60181 aggaagagat tatcagattg gacaaaaaag tgaaacacaa ccMcatgctg tctacaagaa
60241 actgacttta aatataaggg gagaaagata gtttaaaacg aaaggatgga gaaggatatt
60301 ttatgctaat actaatcaaa agaaagctgg agaggctata gtaatatcag atagtgtaga
60361 tttcagagca aagaacatta ccagagatga agagggtcat ttcaagtaga tagtttctct
60421 acatgtttcc ccactaactt tctttcttcg attcctcaat accttctatc tacccttccc
60481 tatgtttccR tgcatcaggg gttctctagt ggagactctt ttcctcccat ctcttaacat
60541 ctgctctgag catccccagc aactcatcag gtggaaaaag ctactagtca cagtttaaat
60601 ctcattctcc caagaaaatg ctcatcttag cacagaccaa agcttgcagt cacaggaaag
60661 gagctctaag ttagaatttc aatgattagt cttctctttc tcaacaacta caaattcagc
60721 accagattat cctcatcagc actgcagatc ccccatggtg ctagtgctag aagctggaat
60781 ccatgttttt cttgggaaaa tctacttaag gcctgttgcc ctgtgctgca aatctctggg
60841 tcacatgcat aaggaaccac attcattgac ccttaaccaa tgaacgccaR tggcagatcc
60901 ctcattctga ttaggtctcc tttcttcccc atgtgcctcc tctaagggtc acttgttggc
60961 tattgaggtg atgggttggt tagtcagtcc ctggtgcaca tcagtcagga tttccttcct
61021 ggcttgaccc tgctcccagg gtcttagggc tgggaagggc ccaaatccca ctaaactcag
61081 tcactcccag acattgtgct ttcccacagg tgatgacagg tcatggacct agaacaggag
61141 tgaggtaaaa ttgaagttta gagcagggag catgtgttct ttttatcttt gtaaggcttg
61201 tagcaattct tcaggtatta tacacctaag agctaaatat ttgaatgtgt agttaaaaag
61261 aggtaggctc tctgccattg catgggcaac ggatggctca tacccacttt cactctagcc
61321 tgttacctcc tctcaatacc ctctgggctg gtcctcatgg ttcctcctga cccctgcagt
61381 tttcttctga ggtgctgagc actggacagc cacagcaagc tgcaggtatt ggcattgtac
61441 ttgttttagc atcaggtgaa catttggaaa agtgaatcac agaattatcg tattttttgt
61501 cctttgtatt ttatcaggaa cctcggagtg atggtgtgtt cctccctgtg tgacataggt
61561 gggataatca cccccttcat agtcttcagg ctgagggagg tctggcaagc cttgcccctc
61621 attttgtttg gtaagatttt gtggagcata tatcattcct tcttttgcag ctYggcagtg
61681 ggctcagcat gggtggaact gagtgtgagt actcagtcgt atttgttgag tgaagggaat
61741 gatatctctc agtgagttta cagaaggcaa ggatgatgca tgctcttggg atgtcttctg
61801 gcttattctg tcttgcttca tgggaaagat aagaatccat tcctaggata tgggtcaggg
61861 atttccatcc tctggctctg gactccaatc attcatacta taggacagaa tctgagagac
61921 cttttgacag tctcctgata aatctgatca ccaattctta cacatttaac acacaattgc
61981 tagagtcttg aagatgctga attatcacct gtcttctgta tctagatctt aacaattgag 62041 aacactcaca aggagatgag tgggaatcaa agttccgtat cagctttctt cctgatacag
62101 ttggatgaga gaagggagag gggattccta cttatctctc caaacctagg ccagagaact
62161 tccaagaccc tgcttaccca cttgatagaa gacaacagtt gctggttgtt tgcagagcca
62221 gccatgcctg ggcacaggtg aatacaaaaa gaggggtgga ggaagagata gggaaaggga
62281 gaaaggaaga aggagggaga aagggaagta gaaggcaaag ggaaaaagga gggagaaaag
62341 agtgatggga ggggtccacc ctgcccacat atagactgtg ctctatagMt aattctatgt
62401 gtatatgtac cccaacaaca aatccccaaa tacttaaaca tcagttacta tggaacttta
62461 cacttctata gaagtaaaat gaaggcaatg tttcctttac gtactctgac atttccccag
62521 ttatcctatt gttttaattt tcttttataa atgttcctaa ctttttttct ttttaaaaaa
62581 tagtatctct Sccatctgtg ttgtctcttc ctctctttgg ctggctgtga ttatttctgt
62641 aaatgacatt ggctgtgctc taatggctcc atttgcaatc tgtttccttt gaagcggtgt
62701 tgggcctgct tgccgcggga gtgacgctac ttcttccaga gaccaagggg gtcgctttgc
62761 cagagaccat gaaggacgcc gagaaccttg ggaggtgaag cccatggtgc ccaggactcc
62821 gaggcccatg agtctgacac gccctccaaa aggggcctaa tatcactcca cattctccat
62881 ttcctctatc ttaaaagttt aggagagctt tacagatatt tgtatgtctt tttattactc
62941 ccagaactgc tatgaggaaa aaaacccatg atagctactg actttatgta agaaaataat
63001 gaagagcatg acttgacagc tatctgttct caagtgcatg gcagagaatg atttgtctgt
63061 aatgttggct gtggtgcacc aagatcccta gctcacccca agagttaatg actctgagtg
63121 gggaacattt taaaatcaat cttctgtcct ctgagcaatt gttgtctcag attctttagt
63181 aactttSttc acaaaattct tttgacagag agcaaggttt gctggagttt catattctag
63241 tcttagagca ataaaaaagg aaaggaagga acagtgcttg atgaggtgat ctctctcaag
63301 atcctttaca ggctcagttc tgtggattcc acagcaagga tgcatgtgtc ctcgtgaata
63361 gagagagaaa agtctatgca gtcctacaga cttacgattg aacctaggtc acctgactca
63421 gtaggccatg tgactttggg aatgtcattg aaccctatgg agctttattt ttcatttaat
63481 ttccatgcct gcaaaatagt cacaataata cttaatttac agagtttcta taaagatcta
63541 atcagattaa gtaggtgaca gtgactaatc cattgcctgg cacatagcag gtgttcaata
63601 gagctcagat tttctccatt cttcttgcaa atcgtgctat gtccccagtg atcctocaaa
63661 ctaagtgtgc agaagaagca caaaaggaac atgctaaaat gctaaattcc ttggccttct
63721 tggtttttgg tttagaatgt gaatgatgca actcaggaat ctgttttaat aatctctgcc
63781 aacaatgcac atgcagatag gcactctgta cacttcactt ggggaaattc tagatttctc
63841 agtactgata agggcaaaac actattttaa cagtaaatgc attttctagt tgctgatgat
63901 ttccttcttt ttgatcccaa gtgttgtgtt atctgattta aagatatttc attctagtgt
63961 gttaacagcc acttggttgt cttttttttc ttttttttga cttggagttt ccctcttgtt
64021 gccctggctg gagtgcaatg gcactacctt ggctcaccac aacctccgcc tcccaggttc
64081 aagcgattct cctgcctcag cctcctgagt agctgggatt acaggcatgt gccaccatgc
64141 ctggctaatt tattattttt agtacagaca ggttttctcc atgttggtca ggctggtctc
64201 gaactcctga cttcaggtga tccgcccatc tcagcctccc aaagtgttgg gattacaggc
64261 gtgagccacc gtgcctggcc tggtagtctt ttaagatgtt ctgtttaatc ccttcactct
64321 ctcatcctaa tcattcccgc cctgccaggg gaggcaggtg ctgctgccca gagccctgaa
64381 ccccctgtgc atccttggtt cttgacagta ctcttcacac gttataataa ttgtttattt
64441 cattgtctgt cttctctgct ataccaattc cataaatagt agccacagtc tatggccaga
64501 gtgatcatgt tgattactcc ttcttcccca cttgcaaggg gagaatgtca ggaatggaag
64561 tgactgtaac tctagctaag cttcactgtg tgtgtgtcag cYtccctgaa agctcatgtg
64621 catttcaccg aatcctccca aaacattggg aggcagcttc tatggttatc agacccattc
64681 cacagatgaa ggagataatg acatgtatcc ccaaagtcac cggattcaaa atgagttagc
64741 caggaacatg atcaaccttc tacttcaggg cttgtctctt tctggaaagg gccagatagt
64801 aaatatttca ggctttgggg ccctgtggtc tctcttgcta ctacccagtt ctgcccctgc
64861 attggaaaag cagctataga caatgcatga aggaatggtt gtgtctggat accagtaaaa
64921 tgctatttat ggatgttgaa atttgaattt catgtaattt tttaagtatc ttgaaatatt
64981 ttcatttttt ccaactatat aaaatgtaaa aaccatttgt agctcactag ttgtacaaaa
65041 acaggctata agctcgaatg gggccacagg ctgtagtttg ctatgccctt ttcttctttg
65101 ctgtttgcca tccttacttt ctctttgtct tgacacttat tcatttctgt gtacaacttt
65161 gcaacagttc catcatcaac aaactgattg cttatttatt tattttaact ccaactttta
65221 attttgttgt tacagaaaag caaagcccaa agaaaacacg atttacctta aggtccaaac
65281 ctcagaaccc tcgggcacct gagagagatg ttttgcggcK atgtcgtgtt ggagggatga
65341 agatggagtt atcctctgca gaaattccta gacgccttca cttctctgta ttcttcctca
65401 tacttgccta cccccaaatt aatatcagtc ctaaagaatg gtttgtgtgg gctttgtctt
65461 attttgtttt ctttctttta agttctccaa agccctggct atcttccacc tgtgcattcg
65521 atctagggaa agctgttggt gctattggta tcgggtactt aattatctaa aggcgtttga
65581 agatcagtag aatttaaata ttgctataaa agagggctgt gtaattggat tcctagaata
65641 agtattctag aaagaatttt ctagaaagaa aattggtttg attcactctc tgaaacaaaa
65701 tgcctttgca tttgtatgta tctatattgg tgaaaacctt tcaacatgta gattgcttgg
65761 agagtacaat cagccttcca tatctgtggg ttccacatct gtggattcaa gccactgcag
65821 actgaaaata tttgggtagc tggggttggt ggctcacgcc tgtgatccca acactttggg
65881 aggctgaggc aggaggattg cttgagccca ggagttttcg accagcgtgg gcaacagtga
65941 gagcctgtgt ctacaaaaaa gggaaaaagt tagctggatg tggtggccca cacctgcagt 66001 cccagctatt agggaggcag aggtgggagg cttacttgag cccagaaatt caaggctgca
66061 gtgagccatg attatgccat tgcacaccag cctgggtgac agaacaggac cctttctcaa
66121 aaaaaaaaaa aaaaaaaaaa aaaattgtgc agcaaagatg attgcatctg cattgaatgt
66181 atacatactt attttttgtc attattccgt gaacaataca gtataacaac tacttacata
66241 gcatttacat tatattaggt attatagtta atctagagat gatttaaagc ataagggagg
66301 atgtatgtag gttatatgca aaaatgacat cattttatat cagggacttg aacattctca
66361 gattttggtg tccatggggg tcctggaacc aatcctcaca gatatcaagg ggcaactgta
66421 ctggttttta gtatggaaac tctgcccaca aaccatgtcc ttgtgacatt tacgtacaca
66481 tgatagaata aaacatgctc ctcattcaaa caccagaagc accatggcca ttttagggaa
66541 gatgtctctg cccccttgtt ccaggaatat tttattctat ctcactcttg gttttggttc
66601 tccttggtga ttctagaagg tgaatgcctg tgccaaccat gagatatcac agtaaatata
66661 tcaaagagag ttcacttcag gatgagttat gtgcttcctg aaccctacac catgcatgat
66721 tttgttcctg gaggacacaa aaaactttat tttaaaattt ctttacctgt caggcaaatc
66781 acaaagataa caatagccag tcttcagagt atatcaggaa aaagcttcta atgggaattg
66841 gaggcatggt gaaatacacg tggttgtatt atagacactt ctggcctgat ctagctgagg
66901 tcagtgatgg ccacaaggac ttgagatcac ggaagtgggt gacgagcagc actttggttg
66961 tcctttgaga acagacattg ctacttgaaa actcaaatct ttgtccttgg gaaaaaaacc
67021 aaaaaaacaa aaaacaaaga actctcaatg gtggagtgaa cRtagccctt catttcagtg
67081 ttggaagcaa agcccttgcc ctgtatctta tactttctga tgataatgat cacactgttt
67141 actggtcaca aatagtaaat tgtaggcttt gaatgatgtt tttcaaatgt gtattaaaaa
67201 tgtcctctct aatctttctt agaatcctct tggcaaaact tctgaggaag gccttaaaca
67261 aacaaaaaaa agtcaacatt aaaaacagcg acactccctg gtcaaacttc cttatggctc
67321 tgtggacatg tatgaagaaa ttagcacgtg tgctgttctt gcaggataag gcacagctag
67381 tgtgtactta cagcacacag cggcattggt gcgggtcaca gtaaggtttg tctgaccagt
67441 caggaagtga atctgggaga gtggcaggac ccccgcaggc atgtctgctc atgctctccc
67501 aagggaggga gggagcgtaa ttccagggag ggtttaaaca tggagccatg gaaacaaagt
67561 gtatttcatg ttgcctgatg catcatgaag gggacattgg ggacccttcc cttcctgcct
67621 tcctcactgt tgctgtgaca gagccaaagt ctgtcttgat aaaaatgggg ggtggcgaag
67681 ggcgtctggc agtgtgaagg cagaaataag tgggttggga gccagatggc attcagatgc
67741 ttggaaaggc tagtagctgt gacccgggca tcaggaaaca tacaaagttc ctggtcaggg
67801 ccttgctgtg aaatgcccag gcccctcctt caggggcagt gctgtggagt tccctgccat
67861 tccccaagca tgtgacagga gccaggggat gccccagccc tcctcggctg gtaagagagc
67921 ccaaagatag tcacagcaac aaatcatgca agaattctca tagcagtggg gcgcactcag
67981 gtggtgctga gaacatcaga gcagcacaac tgaccctatg cgtagatgga ggggatctgc
68041 agatccctgc aatcacctcc tcaatcccgc cccaacatgt cttcatgtgg gtcttgcaat
68101 gaaagaagta aaattttacc ttggtcagca agtgatgggt gtggggggct gcagatcagg
68161 aaagagcagg atggggtggt agccgggggt ctggcggtcc agggatgggt gtctgacgag
68221 agcaggagtg acggcgatgg gaggaggctg tcactttctg cctgttgctg aggacgcagc
68281 tttctgtccc ggaaggaggg aggcagcttt ctgtcccgga tggatactgg accctggcat
68341 gagctggatg tgtttccagg gtctcctcca ccctggagca gggtccttga ccatctgaga
68401 ctctgtctga gactccgcct ccttacggca cctcgctccc agtggttgtg aggattccag
68461 gagctgcagg cttcccccag cagagcccca gagtcagtgc gggggctgct gctgcatcag
68521 gtggcctccc agccacgtgg tctcatgatc agcagcttat ggtggattca atgatctcca
68581 gctgaggaaa gaatgactgt cccttgatgc ttactcctta tacctggttg ctaagggcaa
68641 tgtgtcctaa tgtccatccc acctgcacct cttcccagca ggtggcaggt gaatgtctga
68701 tgttttgaga- catcaacaga gcaggcaaat tagagRccta tttgacttaa tttaagaggt
68761 ctcttgcaaa aggtcattaa tgagacatat tgataaagac tcagtcatcg ccacattggg
68821 aagttaagga agctaagttc agagatgtca aatgactgac aagcatgcaa cgggactttg
68881 aatttccatc ttctgggtca gattacagga ggagcaagta taagcttgca aattaaacag
68941 gaaggaggga ggttggaggg agctgtgtcc tctttgggat gagcttcctg gcattactga
69001 ttgtgcattt gtccctggcc ccatgctgct gagcaggaca gagggaaagc ctaagagtgc
69061 cctgggcaga gttcgaagat cccatcccag agcggtgaca ctctaggcat cctgtgctca
69121 cttgctgatt ctgctcttca gcctgggcag tggcatttat cctcttccgg gttcctgtct
69181 gagtagagca tttcatcttt ctatgccatt tgtaaaaagg atgtgtacat ggaaactaca
69241 tacacatcta cacattcaca tacacagcta cattcacaca tggtctggtt tatgagggta
69301 ttggattaat ttcaaacctc tcattgtaga gtacatgctg ctttctctca tcagtctcat
69361 cattcagtta aaataaacat gagaatctgg ttgtctggga gttttgagga atggcaaatt
69421 tgtgactata aaaataacaa tagctcagat gtattcagaa cttgctgtgt actagctact
69481 gtcctaagca ctttcttata ttatcttgtt aaattctcat aacacttcta ttatattggg
69541 taatgttacc taattgcaaa gaaaaggaaa ctgaggcata aagaggctaa ggggtttgcc
69601 caacattacc aggtagtaag cgaagaacct gtagtctcgc tccaaaatcc aagttttgaa
69661 gcaggatact gcatttccaa ctgacatctg ggcaatagca tctttatgca ttaccaagaa
69721 aaaccccaaa ataaaaaaaa aaaattcatt cttttgtaga gaaacattgt ttttttttcc
69781 tttgtgtgaa gtctttattt caggtttgta tttttttttt tttttgagat ggagtttcac
69841 tcttgtcacc caggctgggg tacaatggtg tgatctctgt tcactgcaac ctctacctcc
69901 caggttcagg caattctcct gcctcagact cctgagtagc tgggattaca ggcacgccac 69961 catgcccagc taatttttgt atttttttag tagagatggg gttttaccat gttggccagg 70021 ctggtctgta actcctgacc tcaggtgatc tgcccacctc agcctaacaa agtgctggga 70081 ttacaggcat gagccaccgt ccccagccta tttcaaatat ttgttaaatc accattgcta 70141 ttcctcagga caagagcaaa aaacaccccc cagagcatag ctaacatggt cccagattgc 70201 agtttctgtt tctggaaggt tcaggtacag tgcgggagtc catttgtcta cttggctaca 70261 ccaccggctg aggtgtcatg gggtcaatgt cactcaagtt cctggcaaac caaactcctg 70321 caaacagtcc cagattgagg atcaagttcc tctggaatca ctcctatcag tggcaaagcg 70381 gtggacaccc cacgactatc tccgccattg tcaggaattt caggagcttc attggctaag 70441 cctgcagcac tcagggtgac ccggctggaa ggcagagtag tcgagaatca ttcttttgga 70501 gacaagactg aaaaggcttc cctggctggt ctgagcagga Ygttctaagg gtcgctgctc 70561 cttggtggtg tgagaagcac attctctttg gaactgcagt aactaagcac ctagctgcaa 70621 ctagggctat ggtgagtttg cctcgattat tgttaaattg cagtttacct gacacctcac 70681 ttgtgattta gtttaaaaat tttaaattac caagaaacgg gggaaaaaaa gtaaaattca 70741 ctttaaatcc caccacttag aagaattcca tggaggaaaa ctgaaggtcg gtcaccgccc 70801 cgtctccccc accccaggga gtcagtgttg acaggtggtg agtttccacc cttcctctct 70861 acagacggag cttgctgact aaggaaaaaa aatcaagctt ttaaagaatt aaagtttgct 70921 ttattcagag gtcttactga ggactacagt ccaggagaag attttcagag agttcctgtc 70981 aggctgttcc agaacaccat tttggctcag aatttatatg cggatcatgg aggctcagtg 71041 caaaatcaca tcaaacttgc ttataagtta catgaaagca gaatcaatcg catcaaggtg 71101 tgggagcagg agcacatctg gttatagagt acagtggcac agtcagcagc cccatcagac 71161 gttatcttcc gtgtgggaaa aggcaaggct aggggcattc aacatttttt ttttttttaa 71221 ttttattatt attatacttt aagttttagg gtacatgtgc acaatgtgca ggttagttac 71281 atatgtatac atgtgccatg ttggtgtgct gcacccatta actcgtcatt tagcattagg 71341 tatatctcct aatgctatcc ctcccctctc cccccacccc acgacagtcc ccggagtgtg 71401 atgttcccct tcctgtgtcc atgtgttctc attgttcaat tcccacctat gagtgagaac 71461 atgcggtgtt tggttttttt gtccttgcga tagtttgctg agaatgatgg tttccagctt 71521 catccatgtc cctacaaagg acaggaactc ttcatttttt atggctgcat agtattccat 71581 ggtgtatatg tgccacattt tcttaatcca gtctatcatt gttggacatt tgggttggtt 71641 ccaagtcttt gctattgtga atagtgctgc aataaacata cgtgtgcatg tgtctttata 71701 gcagcatgat ttataatcct ttgggtatat acccagtaat gggatggctg ggtcaaatgg 71761 tatttctagt tctagatccc tgaggaatca ccacactgac ttccacaatg gttgaagtag 71821 tttacagtcc caccaacagt gtaaaagtgt tcctatttct ccacatcctc tccagcacct 71881 gttgtttcct gactttttaa tgattgccat tctaactggt gtgggatggt atctcatttt 71941 ggttttgatt tgcatttgtc tgatggccag tgatgatgag cattttttcc tgtgtttttt 72001 ggctgcataa atgtcttctt taccaatgac tttcttcaca gaattggaaa aaactacttt 72061 aaagttcata tggaaccaaa aaagagcccg catctccaag tcaatcctaa gccaaaagaa 72121 caaagccgga ggcatcacgc tacctgactt caaactatac tacaaagcta cagtaaccaa 72181 aacagcatgg tactggtacc aacacagaga tatagatcaa tggaacagaa cagagccctc 72241 agaaataatg ccacatatct acaaccatct gatgtttgac aaacctgaga aaaacaagca 72301 atggggaaag gattccctat ttaataaatg gtgctgggaa aactgtctag ccatatgtag 72361 aaagctgaaa ctggatccct tccttacacc ttacaaaaaa ttaattcaag atggattaaa 72421 gacttgtgtg ttagacctaa aaccataaaa accctagaag aaaacttagg caataccatt 72481 caggacatag gcataggcaa ggacttcatg tttaaaacac caaaagcaat ggcaacaaaa 72541 gccaaaattg accaatggga tctaattaaa ctaaagagct tctgcacagc aaaagaaact 72601 accatcagag tgaacaggca acctataaaa tgggagaaaa ttttcgcaac ctactcatct 72661 gacaaagggc taatatccag aatctacaat gaactcaaac aaatttacaa gaaaaaaaca 72721 aacaacccca tcaaaaagtg ggtgaaggat atgtttgttt tgtttttttt gtttgtttgt 72781 ttgttttgag acagagtctc gctctgccct gcaacctgga atgcagtggc atggtcaggg 72841 cccactgcaY catccacctc ccgggctcaa gagatcctcc cacatcagcc cctgctagta 72901 gctgggacta cagggactac aggcatgtgc caccatgctg ggccaatttt ttgtattttt 72961 agtagagatg ggggtctctt gccatattgc ccggctggtc tcaactcctg gactcaagtg 73021 atctgcccac cttggcctcc caaagctctg ggattacagg catgagccgt tgcgcctggc 73081 caagtcattc aacttttaag gaaagacgcg actcaggcaa gaggcatggg ggcctctcgg 73141 gctccatgtg ctctctcctg ctttatcttc aaagcacttt tccagggagc tgcacggcct 73201 cccagtcaRg ggctttgtga aacgatgttg gcaagtagaa atgagcacac ctggcttctt 73261 acctttctta caagccgtat acaaaaatat caggcaggat gaccttctga agcaacaaga 73321 atgcgtgttt cccccacact cttcctttca acccctattc tggtgaccat tgccagctga 73381 gtggcctaag gggaaccaga agacaaaggc tgcagactgc tggcattggg cggcaggcca 73441 gctatgacag agtctcagga caccagcacg agtctccctg tcaatggagg agataagagt 73501 tgttgggggc cgggaaagct ggctcctgaa gggtgcatta gtctgagtgg aatttgcctg 73561 tgagtggcca aaatctccaa aatatcaggg gcttaaacaa gataaaagaa attttatctg 73621 actaaggaaa aaaattcctg actaaagaaa gaaattcttt ctcatgttca agaagcttga 73681 aggacagagt gcagtgcttg tgtgggatgt ggcagagaca ggcccaggtg cctgccatct 73741 cattctgtgg acagagcttc ctccctgagg tccacactgg ctgctgggct ccccgtcaca 73801 tgcaggagat ggggtaccat gaggaacata gatcctccct ttaaggggac ttctggacac 73861 tgcctgcagc ccgtcagctg gacctcatgg ccagaatatc tggagaagtg ggtggcttca 73921 ggcccttcct agagttgggt tgctttactg tggaagtagg tgagaggaga tgggaggtgg 73981 ccaagtgcat ctgctctggt tggaggggtg gatgaccttg agggtctgaa gtagggacct 74041 gcttggttgc tgtaggtggc catgacgctg cccacctggg gaccctgagc aggtggacct 74101 gaggcagctc agagattcct tgtgccatgg gggtaggggg agcagcatga tgtgacttag 74161 ggcttgtcat ggctggtgat atggtttggc tgtgtcccca cccaaatctc gtcttgaatt 74221 gtagctccca taatttccat gtgtcatggg aggaacccag ttggaggtaa ctgaatcatg 74281 ggggtgggtc tttcctgtgc tgttctcttg atagtgaata agtctcatga gatctgatgg 74341 ttttataaag aggaattccc ctgcacaagt tttctcttcc ctgccaccat gtaagatgag 74401 ccttgctcct ccttgctctt ttgccctgat catgaggatt ctgcagccat gcggaactgt 74461 gagcccattc aacctctttc ctttgtaatt actcagtctc gggtgtgtct ttattagcag 74521 tgtgagaatg aatgaataca gctgggatga aggttccacc cacagctggg gcacggactg 74581 catagctggg accatgtggg gagaaagaca ccctggaaag gctggcgtgg gcacaggctg 74641 cccaaggagg gacactgcct gcagagccat ggcatgaagc aagcctcccg ggccctatgt 74701 gccctgctct ggggacacca ggggagggga acaacctgca attcatgaaa tttacatgaa 74761 tttatgaagg ggagaaaaac cctgaagtca ctgagcttac tttgaattgc gaagattacg 74821 ttttccactg cctgctggag tacaggcctg aggtaagaac aaagtcacac aattacaagg 74881 tcatattcct tgcacaatta acttttatag ccttagaatt tatggggttg tgccatctgc 74941 tggtctgcag cttgcttttt caaattaact tctttccatt tcagcacaca ggggtctgac 75001 tggtttgagc agctgcatag caatgtattg agtgccatca ttgattgaac catttctgct 75061 ttctgttcct agagtgtgct ccccttcatg tgaatgtcaa tgatggggtt gttagttggt 75121 tctcccacct catcctgttt gtcacacccc cccttagtgg tccaactctc cttgggggca 75181 gccagtggga gcagttaggg cagcgggcag tcccctggct agtcacccag ggcctctgca 75241 ccaggccctc tgcaccacct agcccagacc ctcctgcctg cagcccagta ggatgggtgg 75301 atcagcttgg atttctgtgt gtcttgggtg cttatggcgc ttggcccacc acagagagca 75361 ggtaaggcgc tgttctgtgc gttcctggac tcccctgtgc ttgatcatcc tcttgcacat 75421 cctcttgtgc cttccttttt tttgtttttg taaatgtcaa agctgtagac tctgtgtgca 75481 aaacatattt ttcttgctga accaaatcct gtttgcctca tcttacatat tcattttgtt 75541 ctatttctgt atatatagaa caaaatatgt attacatttt atttttataa atatatttta 75601 atgtatttat aagtgtattt tataaatgta tttattacat aaaatacata aatacattta 75661 ttacataaaa tacataaata tatttaatgt gtaaaataca taaatacata tgtatttatt 75721 acataaaaca catacattta tgtatttatg taaaacattt attgtgtata aatgtattta 75781 tacatacatt tattgtgtat aaatgtattt atacatacat ttattgtgta taaatgtatt 75841 tatacataca tttattgtgt ataaatgtat ttatacatac atttattgtg tataaatgta 75901 tttatacata catttattgt gtataaatgt atttatacat acatttattg tgtataaatg 75961 tatttataca tacatttatt gtgtataaat gtatttatac atacatttat tgtgtataaa 76021 tgtatttata catacattta ttgtgtataa atgtatttat acatacattt attgtgtata 76081 aatgtatatt atttatacat acatttattg tgtataaatg tatattattt atacatacat 76141 ttattgtgta taaatgtata ttatttatac atacatttat tgtgtataaa tgtatattat 76201 ttatacatac atttattgtg tataaatgta ttatttatac atacatacat ttattgtgta 76261 taaatgtatt atttatacat acatacattt attgtgtata aatgtattat ttatacatac 76321 atacatttat tgtgtataaa tgtattattt atacatacat acatttattg tgtataaatg 76381 tattatttat acatacatac atttattgtg tataaatgta ttatttatac atacatacat 76441 ttatttatat atattgcatt ttatattgct tacaacaggg cctacaaaat gatctcaaac 76501 ttgcagggct gtatttgaaa tctctcaata ggtgtgtttc ctaaacatat cacttaagca 76561 ttttgccctg actcaattaa ataactttca tctctaatag gcactatcct tagtcctcaa 76621 atgcaagaga caacgttttt accttccagc ccttgagaag tgtacagggg aggggacctg 76681 tactgtctcc agtgctgcag tccccttggg gcgggggtgg ctgacaaaat agatctcagg 76741 gggatagatg gccttggtgc ccaaagagat cacaacagcc aaatggactc ccaaaaatgt 76801 actgcctgtt tcctgtggct ctcacacaga ccctggctgg tgacactaga tggattcttt 76861 gctagtccca ggagggtaaa atggggatcc tacgagctaa agagcagctg agaggagacg 76921 tgagtaatcc atgaccccat gttccatcct cttcacagca gattctaaca ttgtttcagg 76981 ctagccctgg acaaaaaggc tgaactattc agagcatcat gcttgggcat tcagttaccc 77041 tagtttagga gagacacttt cagttttcat ctatccctca agggaacctc tgactgtgac 77101 tagaatttcc acccagtctc gggtggcctg taatatatca gcacaccctt catttcccaa 77161 ctgctctctt tcttttccaa gcacagcacg cattttcttt ctagccctaa acactggctg 77221 ctcataaaac caacccctgc cacttattgt ccagctcaga ggtccagacc tctctctaac 77281 tgttccagga atgttcaggt tttatcagct catagtccca gacatgagca ttattaataa 77341 cattcacaat tgctcttctt gacaaagagc tctttaaaca ttggatcgtt tacccgtggg 77401 aggcaaggct tttgtggcct cattttcatc actcttaatt tgtactttta gataaatgtc 77461 ttgaaaatat ttgcaatttc accccgattt agtaatcata gacactggtt tgtttaacca 77521 tctatttgtt ggacgtgcac tgcagcaatt gagatgactg tccggtttat gggtgcccag 77581 agagaccccc acttccagtt caggacatag agaggaggct gccttttgct tgtggctgtg 77641 gccccccagg gtccctgggg ttcatctaaa cctcactaat ggagctgtca aactttacct 77701 ctctcctcaa acccaagtac taaactcctc ccacccatgt attcatttag gcctttattt 77761 agaaaacact cattgagtac ctgttatgtg tcaggcactg tcttagttaa tgaggattca 77821 acagtaaatt aaaaacaaaa gaaaaaccaa aaaacctccg ccctcagaga agttactttc 77881 tagtggggga aaacagacca aaacaaaata agaaaatgat attaaatctt tagaaaggat
77941 caggccttgt ggataagaat aaaagagcag agttcagcag ttccagttgg caaaagctgg
78001 gaaggaagaa atgatgaatc ttgtccagga cttgttgagt ttgagcaaag atgtccaatg
78061 ggtggtggga gatggctttg gggcttgagc tcatgtctgg agagggagaa acatcagcat
78121 ttgatggata ctgaagacag gagaatggat gagcagtctg gaatgagaaa agatggcgtt
78181 cagaacaaaa cccaagatgg aggacagaat agagacttca taataaagac ttgggaaact
78241 gctgaaaaca aatggtagtg tgggatataa gtcagccaat gatcatcatt tcgctgctat
78301 ttgggcaaaa ccctgtgctc tctcgctcat tctgacttaa aatctagcag aaaatatgat
78361 gggcacttgg aaggaaatcc caacctattc agcaatttag tgactacaac atactaggca
78421 gctggtggct tcattctgcc atatgtaatt tgcaagatgg tcttgggtcc tgtctttgtt
78481 ctggagggga tgaggagcag ggtgcctatg ttctagttct catatttata aaatggggag
78541 ggattttcca ccttacatcc aaggaggaaa aactaccaga agaattatac tagcttctat
78601 ttatttacca tctcattttc ttatgtgtgt tctaaacata tacacagttg aagcaatatt
78661 ctgatagaaa cttctttttg tctttaaaat tgctgtccca ttcccataca tgtcagtagc
78721 tggccacagt ggcatccctg cacccttgaa tagttaagtt gctcacggcc acagtcttgc
78781 ttaaatattt tcaaattgct ggatgacatc ctttttttaa aatttttatt tttagagaca
78841 aagtctcact atgtctttca cccaggctgg agtacagtgg tgcagtcata gctcaccaca
78901 gcctcaaata cctgggctca aacgatcccc tcatctcagc atcctaggag ctaggactac
78961 aggcatgcac caccatgcct gagtagtttt ataaaacatt tttttgttgt tgttgtcgtt
79021 gttatagaga cagagtctcc ctatgttgtg caggctggtc tcaaactcct ggcctcaagc
79081 aatcctccca ccttggcttc ccaaagtgct gggattacag agatgagcca ctatgccttg
79141 cctactctcc tcttttttat taagaaggaa aaatgaagat ttataaagct ttggataata
79201 ttataatgta gagatgtggt ttggtgggag gaaataaagc atgatacaac aaagttaacc
79261 aaaaggatct tttatgtaag agaattaaat agtagagaag aaaagttgat agttcactag
79321 ggaaatttat ttttacatga gaacatgata cgaaaggact tagtaaaatc agtcaaatgg
79381 ggaactctac acgtgctatg actttgaagc cttccctggt cccctcagtg gctcgggctg
79441 cctctgatac ggggccgctg tggctggtcc taaagacaag aaaaatgcaa agttcaagaa
79501 ggtcagggaa atgtttttgc caaagagcaa ggactcttgg agatgtctag ggtagtggca
79561 aMagacaaag atgcctaaat atggatggtg cccaatgcca agcggagaca atttcaacat
79621 cctgatgaca gtggttaaag tcaagcagaa caagttgagc ctataaacaa gtccatgata
79681 atactcagat ggaacaaaca gcaagcacgc caaggaaaaa gcacatgagt ttccaataaa
79741 gtttacaagc tgttcaatta gttcaacaaa actgatgtga gccctccact caaggtgaaa
79801 cacccaagtt aataatcata aataagcaga tgtagcgagg aaataattaa agcatgctta
79861 aaatatatga tgtctatggt ttgatcattt taaattacaa tattttggcc atccagtatt
79921 gtagtgactt agaaaccaag gccaaatctt tcagaggctc aatccaaatt gaagatgata
79981 catttggaat tgagaatgta gacctgcttt gccccaatgg gaataaatga cctgcagggt
80041 cggaggctgg cagctggagg ctagaggccc cgaggcattc ccctcgctgt ggctctcaag
80101 gctcagattc agctactggc caacaagcca tggagttatg ccttgggcag aaacagccac
80161 agaactgttc atcactttca aagataaaaa aaattaaaat ggtatcttaa aacgttttag
80221 tttaggctgg gcacagtggc ttacacttgt aatcccagcc atttgggagg ctgaggcggg
80281 tggatcacct gaggtcagga gttcgagagc agcctgacca acatggtgaa accctgtctc
80341 tactaaaaat acaaaaatta gctgggtgtg gtggtgggtg cctgtaatcc caactacttg
80401 ggaggctgag gcaggagaat tgcttgaacc tgggaggcga aggttacagt gagccaagat
80461 caggccattg cactccagcc tgggtgagag agtaagactc catctaaaaa caaaacaaaa
80521 caaaacaaaa aacaaaactc tttagtttat acttaaaaat ctgaattaca agacagttcc
80581 tcctacctta gctgtcatac tcctaacggt aattacagtc aactcttcaa atggtacttt
80641 aaattccagg ctagaactgt aagattcaga aattcccaca gccccaggct ccctctcaac
80701 ctccctgctc agggagggtg aggtcgggtg atgacacaac ctgcacaaac ctgataaagc
80761 caccagaaag ggacctgggg gctgaccact gaggaggtca aaatggtcac catcgttgtc
80821 aaattggaaa acagtaatca gatgtatgca cttaaaacaa taagtatttg gggtttttta
80881 aaacaaaatt tgagggaatt ctttgatgct gatttactga ttagagttag ccaaaaagtc
80941 tactgaaatg ataaaaaccc tctggccacg ggaggctgaa cggggaagag gtgtggggtg
81001 ggtggctgag cggggaagag gtgtggagga ggcaacagtg ttcctggacc aaactgaggg
81061 tggggttgct tattctcgca gctcagtaat gagatgcaga tgaactggga aagaagagaa
81121 tttacttcag taaccgggta cagggagaag ggtgggaaaa tatcaccaga ccgactcaaa
81181 attataatgt tttccagagc ttatagacct tctgagctat atgtctatgt aaaagtgtac
81241 attcttctaa agacgtaagt gattaacatt ttctaatctc taaagtctga gtcctgaaga
81301 ccttcctccg gagcctcagt aaatttactt agtctagatg gRtccaagtg ctagggtgat
81361 tacccttatc ttgtctcctg ctaaatcatg gaggtttggg gaattccttt agaccccaRt
81421 aaaacttgtt tgtggatgtc tggggaattc catcagaccc cagttaaact tgtttaatcc
81481 taaacgggtc ctgttaagaa ttccttaaaa aaaaaaaatt cctttgttat cttgtcatgc
81541 ttcaaggccc aggaaaagcc taggcaaaac aaaaggtggg cttttgttac atatcagcct
81601 ttgtataggg acagaggctc tatcagcttt taatatttaa cttaaccact cagtcagtgc
81661 tcaaacaatt gtcatggagg tctgcccgtt cagctgttag tgagacctag cctgccacaa
81721 aaggaagagg tatctatgac ctaaaccaag ggcatgaaga aaattgtctg gagatttggg
81781 aaatataact tgtgatccac agtcatttaa aatggggagg ttctagagac ttccttttcc 81841 agggtggtcc cagggatcag ctgctcttga catgactggt ggtgttcaaa gctcaagaca 81901 cagcccactg catccctacc acatggtctc tgattttgca gggtccaaac caaaggctgc 81961 tctctttgta tgagaaaaaa catgtttaag acgaatgagc ctggggctga tacctctacc 82021 ccaaatgcca atatgcatga gtggcccttg aagccttgtt cctgtgctgg gtttgttcta 82081 aagaacagtt gtgcaacatc tgactactac tatccaagga aaaggatagg tcattccgac 82141 atttggtgca gaaactctgg ttaatttggt taaaggagca tgtcttctgc cagtgagagc 82201 cactgtgtaa aggatgcaga gacttgaatt aatgatcagt taaaagaaaa ccgtgcgctg 82261 ggtaattttt aaaattcaac aaggcaaatg tagttggtga attaatgacc tgtttaagac 82321 taatgaatga tgatcttctg atagcaatgg taaaaaatgc tcttacctgg gtattcttgg 82381 atcattacaa aattaaccaK cattctgggg ctacacagcc aaccattatc tccccccacc 82441 aacttttatt ttgtcttggg tagagtccac ataacggaaa actatggctc aagaaatttc 82501 ttacctgttt tcattaattg atgcatccat catccaacca tccaccatga actcagttag 82561 tagttgttaa ttgaccatct attatgtgcc aggcacagtt ctaggtgttg ggctttcagt 82621 tagagaacaa caaaggcacg atctcttgcc ttcattgaat ttatattcta gttgggggag 82681 acagactaaa gacattaaga gatgatcaaa atactttcag aaggagataa agtcagtcag 82741 gatgatagga tgcagaaaga cttgaataga tgcccggggc taatttagtt taagtatttg 82801 tggaactatt ttttcttttt tcttgaatag agagtattaa tacatgttta ccatggctcc 82861 tgaatgagtg tatgatcccc tttttctatt gaagaaggat taatattatg taatttcatt 82921 gatttgaagt tcttttctta gggacctttc tggatcctgg ttataatacc cctcccaaac 82981 ctacttaggg agaagtttat gtcttagttt aaggcctgtt cttgttcagt aaactctgct 83041 gacattaaac cctagcaagg ctgtgaatac atttctttat caagttacaa tttctgtttc 83101 cttaaccttc agttgctgga atggaatggc ccccacattc atacatttta accctaggcc 83161 atgggcacac tattttattt ttctgcttct aataggaaca gcagctgagg gccctagccc 83221 tgctcaaagg cttgaaattt cccaagcaRa agctgaaaga aatacaagtg gtacagagat 83281 attgtctggg cgttgcattt tatcaggaaa aaagagtggg tggaatctgc taaccagtat 83341 cagactatca atgccaggaa atttccattt cattgtgagc aagagaatca gagcagtctg 83401 gtccagagac aagtagcaaa tgcaatttgc agcaagtgcc aaaacatggg gcaaagggaa 83461 actagagcaa atctaaactt ttctattatc cagaggtctg taccccaaag atctgaagtg 83521 tgactcacac attgtgtgtg tgtgtgactg tggcagtgac cttgtccatg ggtcttctac 83581 aaaatcatat ttatgtgtca ttgagaaaac catctcatct actaattctg aaaaagtgta 83641 gattttgtct acaaaaaaaa tgaagaatca gagaaaagat aaccaaccat taaaaccatt 83701 ttaggagttc aatacaaaat gacaaacctg agatatcata gagttatatg aattccccct 83761 atgaaaaagg ggaacatttt tgaaaatctc tatgcatgct ctttccaaga ttctagaaga 83821 caatgaagtc acagaaaggg aagcatttgt tagtacatat attgctttta gaaaaaaaaa 83881 ccatctggaa aagcaacaga agcaacgacg agtagattga atattatgga aatcaaattt 83941 gggaattaga agataagctt gagaaattct gctagaatgc agagaaaaga gacagaaatg 84001 aatatgacga gtaaaaagat aaagcacaaa gaaagcagac acagtagatg ccatgtgcat 84061 ctgcagaggg gtgaagtttg aaattaactc ccatttgttt tgctcctgtg cttgaggctt 84121 agcctgaatt ccacaaaagc agaggggaat ttgatcaggg aaggaatccc aggRatgcca 84181 ggagtgtggg aagagagagg gcRtggagct gacaggagga tgcattattt tggtgatctc 84241 cagggacatc tgatcaagtc ccaagaccat gtatttattt atttattgat tcatttattc 84301 atttatttat ttatttttga gacagtctca ctctgttacc caggctggag tgcagtggct 84361 tactgcaacc tccacctcct gggttcaagt gattctcctg tctcggcctc ctgagtagct 84421 ggaattacag gtgcctgcca ccacaccagg cctgaccatg tagtttgatc acaccaaagt 84481 tattcctcga gcttgggaga gcctggagca ccacctaaac attggagtat tccatcaata 84541 ctctccaact tacatgacaa tattctggta gaattattta aatgtgaaat ataaaaccat 84601 ttgaaatact agaatatgat aggggccaac atttatgtaa tttttattca agcaagactt 84661 tctaaacata ataccaatta agaaaccaca aaggaaaaca agtatagatt tgtccactta 84721 aaaatttaaa aaattttctc tctatgaaaa ataccaaata aaaaactaaa gggcgaatga 84781 ccaactccaa gaaacaattt gaaaaaggct ctgacggaaa aYagaatatc ccagaaggaa 84841 aatgggcaaa gaacaaaaat agtcacttca taatataaag aaaattagca ataaccatga 84901 aaacatgttc aacctcactc ctcaaagaaa ttcagataaa atggccagga gatattattt 84961 tgtgtgtgtg tgagtgtgtt gtgtatgtgc atgtgtgaga cagagagaga gaaagagaga 85021 gagagagaga gagagagatg agaagggaga gggagaggga gggagggaag gagagagaga 85081 gagYgcgaga gagcgagaga gagcgctgtg tgtgtgtggc aaagaagaca tgaaaaagcc 85141 acactctggc attgtgttgg tgaaattaat ttgtaacaca ttttgaagga caagctgatg 85201 tttatagcaa aagtcttaaa atgttccagc acatagtaaa gagtaaaaaa aactccctga 85261 ttttcgcaat acccatactc ttaaccactt ctttctactg cctcagaaaa tgattgcaag 85321 taaaaatgta aagtaactgc cctaaagaca gttcagtgtt tccctccatc cccctctctc 85381 cctgtctttt cccttcacct tctactctcc tcctcaccat agcacaatga ccgactttgt 85441 tccgtttaag aatcctgttt aatgaatgta tggtactcta tttaactagg gacactgtat 85501 tttgtactgc tgttaccaac tcttgcctct tatggaatac tttagagaca atcactccac 85561 tagtcttagt tagcagagct ccttagagcg aaatttaaaa gcagggttgc ggggggtggt 85621 ctcctttttt tttttttttt ggagtttccc ttggtcctct catcaagtta attactattc 85681 taagtatact tcacgtggaa cagttattct cttgtaccta gaacagagga aatctttatt 85741 ttaaaatgtg agctaagata acgctgtgtc agtgtctgac cttgggcggc tcatttccca 85801 attaggaaaa ctggagacag ccagKcatgg ctttggagga gtcccctgaa agcaccgtag 85861 ctggtctccc ttgaatatcc aggcacagac atttccctgg cctcYtgact tggtctgttt 85921 gggctggtat aacaaaatac cttggactgg gcaatttata aattattata aacagaaatt 85981 tattgctcag agttctggag gttgggaagt ccaagatcaa ggtgccagca gatttgtttc 86041 ttggtgaggg ctctcttctt cacagatggc accttcttgc tgtgtcctca catggtggaa 86101 gagcaaagag gaaccgacag gcttccccag gcctctttta taagggcact aatccaatca 86161 tgaagggttt gccctcatga ccaaattatc ctcaaaggcc ctactttcta ataccatcac 86221 ttaggggtta ggttttaacc catgaatttt ggaaggatac aaacattcag accatagcac 86281 ctcctaacaa gccatggcta aaagaaaaaa gctacctacc ctcttggctg tgagaacaca 86341 tttttgtgca ttttgtaccg acccatgtta ttaagggccc tacacagaaa gcctgggaca 86401 gtggatggag tgttgagacc ctgacatgcc cagttaagtc ctgatgtgag aaccccagcc 86461 agggaacatg gggacttgga aaatacttct tgaatggcag cagccttcaa gacaagagtc 86521 agccaacatc atagttaggt caaaaggttg atgctcagag tctaaatggt ttttgtatat 86581 gggccattgt aaaacacata tttacacaat aaatactgtg aaacaaaatt agaacaggcc 86641 tgtttgtggc tcttaatagt acaaattgga aactggagaa aaaccgtgat ttatttaaaa 86701 acatttttgt aaaagctttt gccttttctg gagtgtgtct cttgatgata cataatgatt 86761 ctattcaagg tctatgacta ttacatgaaa aacactacca cagtcacaca cacaaaaatc 86821 tgtcaagtta attggaacct ttttgtattg ttggtggtgg gaatgtaaaa tgttgcagct 86881 actgtggaaa acagtatggt ggttcctcaa aaaattaaaa atagaatgac catatgatcc 86941 agtaattcca cttctaagca cRtaccctaa agaattgaaa gcaaggtctc aaagagatat 87001 tggtacacat atgttcatag gagcattatt cacaacagcc aagagatgca agcaacccaa 87061 ctgaccattg acagatgaat gcatgaacaa aatgtgatgt atacatacaa tggagtgtta 87121 ttcagcatta aaaaggaaga aaattctgac acataccaca acgtggatga accttactaa 87181 gtgaaatgtg tgaatcatgc caaatgaaat aagccagtca ccaaaagaca aatacggtgt 87241 gattccactt atatgaggta cccagagtag tcaaattcat agagacaggg tagaatggtg 87301 gttgccaggg gttggggaag gggagaatgg agagctattg cttcatgggt atagagtttc 87361 cattttgcaa gataaagagt cctggagaga atggtggtga tcattgcaca acaatatgaa 87421 tatatttaat actactgaac tgtacactta aatgtgatta agatgctaaa tatcttgggt 87481 gtcttttatc acaataaaaa atatatcaag ctaaatgccc cacagtgctg tggtgagtta 87541 tttctgtgca tggcaagata gtctcaagta acagataaag ttaggttatc tctcacatat 87601 ttgacttcca gctggttaat tcccatttac cctgcagatc tcactgaaaa cattgctttc 87661 ttcaggaagt cctccttggc tcccagccta ggccaggtct ccctgtaaca ttcacttctc 87721 ctctgggctg ccgtttctta agtgcattct ttgtgtcagt tgttgctcta ggtgagtatg 87781 ttcaattcac catgataacc agaactgtaa cttcttgctt aatgttttct cttctgctac 87841 aacatgaatt ctatgatcca ggaaaagaat ctgccttgtt ctatgctgtg tctccatgtt 87901 cagtatagta cttgctagac acttaatgag tattggtcaa ataaacacta ctgtgaatgt 87961 caataaaatg tctacttttg cttgtctccc tgtagatttt aatactgctt ttctcttatg 88021 cattgagatt ggcagaggtt tttctgtaag atctatagat aacatcaaga atacagtttt 88081 aaaaccagaa actcttaaaa atattaatgg caaatctcat ttagccattc ttggtagctg 88141 ccagaatccc aaacatgcat ttctagtagg ttctcatgtc tttggggact gactctgctg 88201 tgtttcttct aggcttctaa agacttaact aaatagaaga tggccattaa tgtccttcct 88261 gatgttttta tacaatatcc tttacttttc aaggacagaa aaggcttgaa ctttggatca 88321 tgtagtaacc aaaggtaggt catctttatt ttattttatt ttatttattt attttttgag 88381 acagagtctc actctgtggc ccaggctgga gtgcagtggc ttgatctcgg ctcactgtaa 88441 gctctgcctc ctgggttcag gccattctcc tgcctcagcc tcccgagtag ctgggactac 88501 aggcacctgc caccacaccc agctaatttt ttttgtattt ttagtagaga tggggtttca 88561 tcgtgttagc cagaatggtc tcgatctcct gacctcgtga tccacccacc ccagctccca 88621 aagtgctggg attacaggcg tgagccaccg cgcccagctg gtcatcttga tttagactga 88681 tccccaaagg gttcccagaa agtctggggt tcacccaaag tttataggcc aaggagtatt 88741 tgtgtttctc cctctttttt tttttttcac tctactctta aagctgtttc atatgatccc 88801 attttaatga cagcttcctc caatactcta gactgggcct gcaatacgtt tagcatttca 88861 gggagtttac attattgtag ttttaatccc agaatcaagt taattactat tctgtcagaa 88921 tcagctgcca gaatatttat tacttcagtc aatcgaagct ggccattcaa aactttgttg 88981 gtgaatactt gctttttttt tttttttctt gagatggagt cttgctctgt ctcccaggct 89041 ggagtgcagt ggcacgatct tggctcactg caacctctgc ctcccaggtt catgccattc 89101 tcctacctca gcctcctgag tagctgggac tacaggcgcc cgccaccacg cccggctaat 89161 tttttgtatt tttaccagag accgggtttc accgtgttag ccaggatggt ctcgatctcc 89221 tgaccttgtg gtccacctgc ctcaacctcc caaagtgctg tgattatagg ggtgagccac 89281 cgcgcctggc caaatgcttg cttttttgta gtttctgagg ttagcagaaa gcttggtatg 89341 ttgtttaatc ttactgatat agttaggttg caacatatgc aatgagagtt gctttccatt 89401 ctaaatactg gaacttttct attttaaaca tatatcaaag gaagtaaata ggttatttga 89461 aatcacattt tcaaaaaatt actaccagat ttttagtgtt atcatctgct ttgaatgaat 89521 aaaaacataa agtccatttc atctgcttgc tttccaattt ttaagattac ttattgagtc 89581 aactgaatta acattgtttt ctaacacatc aaaagaggtt gaatcttcaa taaccaaaga 89641 aacctcaagt acatactgat gtgtttcttt ttttagataa acatttatta agttactRta 89701 gctcctttgc tctcttagaa taggaatttg caggatattt ccttggttac taacttgcct 89761 gattagtaat taaattttac tttattctga ttagggaaat acattttccc tcaatgaaat 89821 ggaatggtat atacttgcta caattactag atcaaatatg gccaagaaag acccagaatt 89881 tctccttcac ttatgaaact taatttgSct ggatatgaaa ttctgggttg aaaagtctat 89941 tctttaagaa tgttgaatat tggcccccag tctcttctgg cctgtagagt ttctgccaag 90001 agatcagctg ttagtctgat gggcttccct ttacagacaa gcaaatgctg agagatttgg 90061 tcaccaccag gcctgcccta aaagagttcc tgaaggaagc actaaacatg gaaaggaaca 90121 actggtacca gttactgcaa aaacatgcca aattgtaaag accatcaatc ctaggaagaa 90181 actgcatcaa ctaacgagca aaataaccag ctaacatcat catgacggga tcaaatKcac 90241 acataacaat attaacctta aatgtaaatg ggctaaatgc tccaattaaa agacacagac 90301 tggaaaattg gataaagagt caagacccat cagtgtgctg tattcaggaa actgatctca 90361 cgtgtagaga cacacatagg ctcaaaataa agggatggag gaagctctat caagcaaatg 90421 gaaaacaaaa aaaggcaggg gttgcagtcc tagtctcaga taaaacagac tttaatctaa 90481 caaagatcaa aagagacaaa gaaggccatt acataatggt aaagggacca atttaacaag 90541 aagagctaac tatcctaaat atatatgcac ccaatacagg agcacccaga ttcataaagc 90601 aaatccttag agacctacaa agagacttag acttccacac aataataatg ggagacttta 90661 acaccccact gtcaacatta gacagattaa caagacagaa agttaacaag gatatccagg 90721 aattgaactc agctctgcat caagcagacc taacagacat ctacagaact ctccacccca 90781 aatcaacaga atatacattc ttttcagcac cacaccacac ctattctaaa attgaccaca 90841 tagttggaag caaagcactc ctccgcaaat gtaaaagaac agaaattata acaaactgtc 90901 tctcagacca cagtgcaatc aaactagaac tcaggattaa gaaactcact caaaaccgct 90961 caactacatg gaaactgaac aacctgctcc tgaatgacta ctgggtacat aacgaaataa 91021 agacagaaat aaaaatgttc tttgaaacca atgagaacaa agacacacat accagaatct 91081 ctgggacaca ttcgaagcag tgtgtagagg gaaatttata gcactaaatg cccacaagag 91141 aaagcaggaa agatctaaaa ttgacactct aacatcacaa ttaaaagaac tagagaacca 91201 agagcaaaca cattcaaaag ctagcagaag gcaagaaata actaagatca gagcagaact 91261 gaaggaaata cagacacaaa aaacccttca aaaaattcat gaatccagga gctggttttt 91321 tgaaaagata aacacaattg atagaccgct agcaagacta ataaagaaga aaagagagaa 91381 gaatcaaata gacacaataa aaaatgacaa aggggatacc accaccgatc ccacagaaat 91441 acaaactatc atcagagaat actataaaca cctctatgca aataaactag aaaatctaga 91501 agaaatggat aaattccttg acacatacac cctcccaaga ctaaaccagg aagaagtgga 91561 atctctgaat agaccaataa caggatctga aattgaggca ataattaata gcttaccaac 91621 caaaaaaagt ccaggaccag atggattcac agctgaattc taccagaggt agaaggagga 91681 gctggtacca ttccttctga aactattcca atcaatagaa aaacagggaa tcctccctaa 91741 ctcattttat gaggccagcg tcatcctgat accaaagcct ggcagagata caacaaaaaa 91801 agagaatttt agaccaatat ccctgatgaa cattgatgca aaaatcctca ataaaatact 91861 ggcaaaccga atccagcagc acatcaaaaa gcttatccac catgatcaag tgggcttcgt 91921 ccctgggatg caaggctggt tcaacataca aaaatcaata aacataatcc agcatataaa 91981 cagaaccaaa gacaaaaacc acatgattat ctcaatagat gcagaaaagg cctttgacaa 92041 aattcaacag cccttcatgc taaaaactct caataaatta ggtattgatt ggatgtatct 92101 caaaataata agagctatct atgagagacc cacagccaat atcatactga atgggcaaaa 92161 actggaagca ttccctttga aaactggcac aagacaggga tgccctctct caccactcct 92221 attcaacata gtgttggaag ttctggccag ggcaatcagg caggcgaagg aaataaaggg 92281 cattcaatta ggaaaagagg aagtcaaatt gtccctgttt gcagatgaca tgattttata 92341 tctagaaaac cccatcttct cagcccaaaa tctccttaag ctgataagca acttcagcaa 92401 agtctcagga tacaaaatca atgtacaaaa atcacaagca ttcttataaa ccactaacag 92461 acaaacagag agccaaatca tgagtgaact cccattcaca attgcttcaa agagaataaa 92521 atacctagga atccaactta caagggatgt gaaggacctc ttcaaggaga actacaaact 92581 gctgcccaat gaaataaaag aggatacaaa ccaatggaag aacattccgt gctcatgggt 92641 aggaagaatc aatatcatga aaatggccat actgcccaat ttatagattc aatgccatcc 92701 ccatcaagct accaatgact ttcttcacag aactggaaaa aactacttta aagttcatat 92761 ggaaccaaaa aagagcctgc gtcgccaagt caatcctaag ccaaaagaac aaagctggag 92821 gcatcacact acctgacttc aaactatact acaaggctac agtaaccaaa acagcatggc 92881 actggtacca aaacagaggt atagaccaat ggaacagaac agagtcctca gaaataatgc 92941 cacatatcta caactatctg atctttgaca aacctgagaa aaacaagaaa tggggaaagg 93001 attccctatt taataaatag tgctgggaaa actggctagc catatgtaga aagctgaaac 93061 tggatccctt ccttacacct tatacaaaaa ttaattcaag acggtttaaa gacttacatg 93121 ttagacctaa aaccataaaa atcctagaag aaaacctagg caataccatt caggacatag 93181 gcatgggcaa ggacttcatg tctaaaacac caaaagcaat ggtaacaaaa gtcaaaattg 93241 accaatggga tctaattaaa ctaaagagct tctgcacagc aaaagaaact accatcagag 93301 tgaacaggca acctacagaa tgggagaaaa tttttgcaac ctactcatct gacaaagggc 93361 taatatccag aatctacaat gaactcaaac taatttatga gaaaaaaaca aacaacccca 93421 tcaaaaaatg ggcgaaggat atgaacagac acttctcaaa agaagacatt atgcagccaa 93481 aaaacacatg aaaaaatgct catcatcact ggccatcaga gaaatgcaaa tcaaaaccaa 93541 aatgagatac catctcacac cagttagaat ggcaatcatt aaaaagtcag gaaacaacag 93601 gtgctggaga ggatgtggag aaataggaac acttttacac tgttggtggg actgtaaact 93661 agttcaacca ctgtggaagt cagtgtggtg attcctcagg gatctagaac tagaaatacc 93721 atttgaccca gccatcgcat tactaggtat atactcaaag gattatgaat catgctgtta 93781 taaagacaca tgcacacgta tgtttattgc ggcactacac acaacagcaa agacttggaa 93841 ccaacccaaa tgtccaacag tgatagactg gattaagaaa atgtggcaca tatactatgg 93901 aatactatgc agccgtaaaa aatgatgagt tcatgtcctt tgtagggaca tggatgaagc 93961 tggaaaccat cattctcagc aaactatcgc aaggacaaaa aaccaaacac cgcatgttct 94021 cactcatagg tgggaactga acaatgagaa cacatggaca caggaagggg aacatcacac 94081 atcggggact gttgtggggt gggggagggg ggagggatag cattaggaga tatacctaat 94141 gctaaatgat gagttaatgg gcgcagcaca ccaatatggc acatgtatac atatgtaaca 94201 aacctgcaca ttgtgcacat gtaccctaaa acttaaagta taatattaat aaaattaaaa 94261 aaaaagaaag acccagaatt atgtataaat acaatgacac ataagcttca aagtgtatag 94321 cctgaaaaat ggtgcttcag ggatttaaaa ttccaactgg tgaattgtaa tagaaatata 94381 aattttgtaa atgaaataga tagtgaatta aaaatcaaac ctctttcttt taaatgagtt 94441 atatttttct ggcatagata aatgatatac aggaagactt tcgcttctga cagcatggaa 94501 tagagaatta gaaaaaggac cgccaaattt actcaaagta agaaaaatgg agaaaaagag 94561 cagaaaataa tgaaatacaa aaaaagaaaa gataatgtca aggaagccaa acctagctct 94621 ttgaaaagat taatgaaata gacaaacgtc tggtgagact gatcaagaaa caatgagaga 94681 aggcatgaat agacaatatc aaaatagaaa aggggcataa ttacagatct gggaggtatt 94741 tattttttct ttttaaatag ccaagttcaa gaggaaatat ctgggagata ttacaaaggt 94801 aagagaaaag tgtccacaac tttctgccta tgcattagaa aaattaatga aatgaactaa 94861 ttcctagaaa tgcttaagta ccaaaattca ttgaagaaat aggaagccta gatcatacta 94921 cagcaataaa ataaaatgaa tgagtggtta aatgcttcac cacaagaaaa catcagctag 94981 atggctttga aggcaagccc cactaaactt tcaggcagca gatttttcaa tcctttgcta 95041 acacttgttt ttttttaaat ttattttatt attattatac tttaagtttt agggtacatg 95101 tgcacaatgt gcaggtttgt tacatatgta tacacccatt aactcatcat ttaacattag 95161 gtatatctca tcatttaaca ttaggtatat ctcctaatgc tatctctccc ctctcccccc 95221 aacccacaac agtccctgat gtgtgatgtt ccccttcctg tgtccatgtg ttctcattgt 95281 tcaattccca cctatgagtg agaacatgcg gtgtttggtt ttttgtcctt gcgatagttt 95341 gctgagaatg atgatttcca gtttcatcca tgtccctaca aaggacaaga actcatcatt 95401 ttttatggaa tcaaaacaag tgttagcatg gaatgctaac acttgttttg atttctgtgt 95461 taatccaagt cctctgagaa gcaaatgcca aagcagaatt aaaagtgcaa taattttatt 95521 gggcaaaatt cttgtgatat aaaatggtat ggagctgggg agaactgtca gaaaggaaag 95581 gagaggaaga gagggggcac agaggcatcc tagactcctg ggcagtcaag gagaggtttg 95641 gcagagttgg agggcagatg aatcactcag gaatggacta tctgagtctc cctgcaagct 95701 cagtcatcat ctgggagcag cccaagggaa gcaaggcctt gggcacacac agcaatggca 95761 ctcagagtcc agcctcggct ctgggttagt tttgcttcct cttggtgatc tRtgtatact 95821 catagcagcc tcaattctaa aaccaggagt ggatgagaaa ggaaacatag aagccattcc 95881 tatttattaa tatagattta aaaattctaa acacatacta gaaaaccaca tccaacaata 95941 aataaaaatg ataatgctat gaccaagtca ggtttgtcat acaaactcaa tagtagttta 96001 acgttagaca atctataaac ataattaacc acactagcag gtcaaatgaa gaaaaaatct 96061 tagcaaatga gaaacataag gtattttcgt aacctgataa agtggctggt taacagtcta 96121 cagcaattat catttttaat gatgaaatgt tataattatt ccatttaaaa ttagggctaa 96181 tggaacgatg gtacactcaa taaggcttca acactgtgct gaagtcctag ctagtgtagt 96241 tggagaaata ctggaaaaga agaagcaata ctatcattat taatatataa cataatagtt 96301 tgtgtagaaa ataaaaaaga atctacaaag tgtcaggaat aataagacaa gtacacaaaa 96361 gtaaattgcc ttgctatgtg ccagcagcaa cgacaagaaa aacatagttt cataaatata 96421 tcatttaaaa cagtaacaac aaataaaaga tagctaacaa taaatctaag atttgcaaca &6481 cttgtaagca gaaaattttt aaactttact gaaagatatt agagaatatt taattcaatg 96541 tataaacata tgatgctctt gaataggaag atttaacttt acaaagatgt taattatctc 96601 catattgatt tagaggttta aaacaattcc aataaaaatc ccaactaggt ttggagtttc 96661 taggggaatc tgaaaaatta atatgtaaga gcaaaggcct aagaatagct aatacacaga 96721 agaaggagga ggagaaggaa agaggaggag gaggaagaaa gaggaagaga aagaaaaagt 96781 atacttgctc tggcagatac aaagacttat gataaggata caggtattga cagaatcatg 96841 ttgatgYagg gattgaaagc caaaacagga aagagagctc aaaaacagat gacatctatt 96901 tctatggaaa cggaatttaa gacagagaaa gagtaacaaa ttaStgggta aagaagagac 96961 agttttcttg ccatacacaa cagttaaatc ctgacaaatt aacaacttga gtgtgaaaag 97021 caaatctttg agacttttag aggcttatgc atgttcaggc tctgtgtagt aaaggatttc 97081 ttaaacaaga tattacccat gcaaatctaa aagataaatt tagctgtgtt aaactcaaga 97141 atttcctttc atctaaggac actRgaaaga tagtaaaaga catgttgYca actgaaagaa 97201 tatatttgca acatttataa ctgataaaaa tattagagca atacatgatt tttttttctt 97261 ttttgatgga gtctcgctct gttgcccaga ctggagtgcg gtggcgtgat cttggcttgc 97321 tgcaacctcc gcctcctggg ttcaagaaat tctccctgtc tcacccgccc ccccgagtag 97381 cttggattac atgctcctgc caccataccc agctaatttt tttatttttt agtagagaca 97441 gggtttcgct atgttggcca ggctggtctt gaatgcctga cctcagatga tccacccgcc 97501 tcgacctccc aaaatgctgg gattacaggc atgagccact gcactcagcc agcaagcaat 97561 acatgatttg agatgccagg cactcatcct ccacacaaga aaggataaag gccatgaaag 97621 agagctaagg tttgactgga ggtcgaaggg agagtgctgg agtgtagtgt ggagtcgaca 97681 gacacttctg aagccttgtc tccccttccc agattaKgct ggcatggagt ctggaaggac 97741 ttccaaatgt ggggaaagga aagcagaaga tcccaaccag cccccattgc cattgcaaac 97801 actaacagct tttactacag aagaatccca tagtccttgc aagccctgag cccagtttgg 97861 agagctgcca ggaactcaca cagttgcatt gctccagatt agaagtgcaa agtgtgcact 97921 cccttcctcc cacccactcc ctgtgaagct actttagcat ggcaccacct cttgagttca 97981 ttctactctg acagccagca gccactggac ttttctagcc ctgaggcttt gtcttcattc 98041 caccaagccc atacaggtgg ctgaacacca caactccagc tgtgcagatc ctgggcctag 98101 gatcagctgt gattctagtc ctgcacatta gggaaactaa tctctgtgac ctccacttcc 98161 agcaagagaa acagtctggc agtcccccca aggcaaactt gcccttgagc cagtcaaact 98221 tctatgtgcc ttcccccaag caggaaaggt cctcaagcca ctaagcagct ggcatgtccc 98281 caggctggca gggtgtctac atgcccatac ccagagcctg agaaatagca caggtagttc 98341 ctggcctgcc caaaggccct gtgcccacaa gcagggcctg agtaacagtc ttgtaggttg 98401 cccctggcag acacatcccc aggcagagaa cccccaggcc aattgagcag actggtacct 98461 gtgttctggt cttgagaaac atcctcatgg gccatacctg gtagacacct ccaggctggc 98521 tgtgaaacca catgcctgtg cccccagccg gaataacatc actgtggccc caacctcagc 98581 aagctgaccc aagttggctg acacactgcg tatgtgcaca tgcccacaac ctgagaaaca 98641 gcccagtgag cccacacctg gcaaagttaa actgccaaca caaatggtca tagcctaggc 98701 cactgagaca ctcataaaca tcactggtgt ggattatagc tgaagaaaat acatataaac 98761 aatagtattg tgtccaccta gaaccaaagc caatgcactc caccaaattg acatcccaag 98821 acccacccat atgagtaagt ctttttctac aaagtgcaYt ccataaaatt ggaagaggtg 98881 aattttccac cagatgcata gacatcaatg taggtataca tcaaacatga aaaagcaaag 98941 aaacatgaca cctccaaaag Racaaaataa ttctccagta acagacccta atcataagaa 99001 gatatacaaa attccagaaa tgaaattcaa gataagaatc ttaaggaaac acagtgagat &9061 acaagtgaac acagacaatt ctaaaaaact aggaaaacca ttcattattt aaaagagaag 99121 ttcaacaaag acacagaaat aataacaaag aaccaaacag aaattotaga gccaaaagaa 99181 cacagtgaat gaattaagaa atacaatcaa gagcttcgac aacacattag gccaagcaga 99241 agaaagaatt tctgaacttc aaaacaggct ttaaaaaata tcacaggcag acaaaaaata 99301 ataaaaagta aaaaggaatg aagaaaatcc ataggattta tgggacacca gtaggtaaac 99361 agatgttcac cttagagtgt tccagaagaa gagaYtggaa aaggtatata aaacatattt 99421 aataaaataa taactgaaaa attaccaagt cttgagagag agatggacat ccagattcag 99481 gttgcccaaa gaaccctaaa cagattcaac ccaaacaggt cctctccaag gaacactata 99541 ttcaaattgg taaaatgtag agacatagaa agaattctaa aaagcgcaaa agtgtcaaat
[0376J FollowingisagenomicnucleotidesequenceofaTTNregion(SEQIDNO:2).
>2:179567701-179765800
1 tacctcataa aataataatt ttaaaatctt atttgtttcc tgatgaatca aggttgtcat
61 taagtacagt aaagttgggc tgttacaaaa atagtagctg agatgctgtt aggtggtggt
121 gatttgcttc tctgtttaaa tttttcagac agatttttaa aagagttttt ctaggcccct
181 ttttgccatc tttatcttct cttgctgttg tcagtgagtc tacctctagc tctatgaact
241 cctYgcccat ctctttcagt ctgctggttt tgatcagata tggattgact taaaatacat
301 gcattcatga aacctatgac ttctagtttt caaacatttg taaaccgact gatatgtctg
361 ctctctggag tctcccctac agaagtcact acttcttcat tgtacccctc ttccttttag
421 ttcagctcag aagccatatt ctccaggtta ccagctgtct tctaaatggg ttcacttgtt
481 tttgacttct gagtagaatg ttgctaacac gggacgttga gcataaaaat aattcttttt
541 tcacttaaag agaagtcatt taaaattcca gttgagtgtg tttttgttta tgtaaggcct
601 tacaatttaa tgctttttac ttaatttgca ggagtagcca ctctataaaa gaaccagtat
661 ctacacttca ccgactctcc cagcgacgcc gaagaaccta ctcagataca gattcttgta
721 gtgatattcc tcttgaagac ccagatagta agtgacatag attctgtctc tttctttggc
781 atattcagca aaattactgt ggggctctgt aatttctgga aatgaagtat actgactgaa
841 cattgtactt tgtttctttg attccttaat tcagaggtaa gcaaacagcg ctcaatcaat
901 gaaagctcat atatcccctt acaaacaggt ttgttaYtaa ctaaggagtg ctgaggattg
961 ctttgtaaat atcgtatctc agatatatat ttatagaata tcatgatctt ttaccaccac
1021 taggacttaa aaaacatttt agaagaaggc atgaattgtt agcaggattc taggggaaaa
1081 taaaggcaag ttccaagttc atataaaagt cagcattgtt actttactaa aatacctaga
1141 aatggctaag gaaaagaatt ttgtctggta gtcacagtga attatagtag cattcattcc
1201 tctacctttt tcttccttat taaagaggca gcatactgtg attgaatgtt tttaggaaag
1261 gttttgtgat ttatgttaaa atttttaaat ttttctcttc agagaagaag ctttgaaagt
1321 gtgtcagaat atctagtaag atagtagtga ccatgatttg cagtgaacac aaatatagtc
1381 ttattattac atctccatta atagacttat actctccaag tctctactat ttctcaggaa
1441 gcatgtggga tagaaactat gtacaatttc caattaagga acaggtcgtg ttccaatagt
1501 tagttcattt gtaagtcagt tgtttggaaa acagatttta tttttcttgt ggaaatgaca
1561 tgataagtgg tggttagctc tttggatagc taaccattct ttggaagagc ccagtgaatt
1621 catagtatgg ctgaaatggg acaaaacaat ggtgtatttc tagcatttga gtgtattgaa 1681 aacactgatt aagctggagt tgttttatgt aaccagaaag ttaaacaggt ttagttcata 1741 catatggcaa ttttaaaaag gattgctggt catttttgga tttttaggag ttgatagaat 1801 cttttttttt ttttaatgcc cacttttttt ttaaactgaa ttaatcatct atttattaat 1861 atatggcact attctatgct cattaacatt ttgaatttaa attgagatgt gggtggagaa 1921 atgaaggaaR gaaaccggga aggggaaggg agaaagaaac tttcttgggc caatcaagca 1981 agacctggaa gagaagagga ggagaggagg agggttaaag aggttgagta tgtgaaaaaa 2041 gttatctgag gcaggtaaga tagactctga ttctctttat tctttatgcc ttttacacat 2101 gactcttaat ccagagttca tgaaggagtt ctgtatgcta ccttgtagtt ttaagtgcga 2161 catctttttt tttttttaag gggatcattt gtgattacta aaataatttt tttaaagcca 2221 tccatttttc tttagaattc ttttcttcta gttactctag tcctgaatat ttgaaaacaa 2281 ttttcaaaag atctaggtgt acatgctcta ttatatttag catcccccag tttaactgtt 2341 atggactgta acaaaactag tctcacacac acacacacgc acgcacacac acacacgcgc 2401 atacatatag ttcacttctc tagaacatct ttaaattagc aagtatagac taggtcagaa 2461 attttatcct tagatttttt cctgtttata ctagtttagt gtttcaatta gctgacaaag 2521 caaagattga attagtgctc tttccagaaa gtatatattt tgcaattggc agtcttagtt 2581 gaaagttgtt actcaaaaat attcttcttg tgagatgcaa ctgcttctat tttctttaac 2641 aagctttttc aaaaagtatg aaagtaataa atgatgatgg taaactcaaa ctatatatat 2701 aaaggtacaa ctgccttaga acaggactat gccagtgtag tcagctgtgt taacatggag 2761 ttgcagtgat agttctgtga gctgcattac aaatggagaa atgggatctt tcagtatgag 2821 gtaccttgtc agctttgctc agaaagtctt agaatgaaaa gaggaatacc tgaatttagt 2881 cctcgttctg ttaccaattg tgtgatcttg ggtaaggcat gtatcctttt atgggtcttg 2941 gtttctttgc ctaggaacaa ggaggatatt aacagcatta aaataggctg aaaaagcaat 3001 agttaattaa taaagattaa attcaattca gcaaatattt attgactacc tattatgtgc 3061 caaccatgat tctagcagtg aacaaaacaa aaatctctac ttcaggcaat ttcatcattt 3121 atcttatgaa ttctgatgtt tctccttgtg gctgttgata ctctagtcta aaagttaagt 3181 tttcctgcat gaaacaaaca aggaaataca aaattagcca gaagctaaag gaatttcatg 3241 tccctgaaca ggaaatttaa aatgttcttt cacagaggac tggagttaat attgacagga 3301 tgtttaacgt ttttatcaat gtcttcatag gacctgttca ctgttcaaaa aatacactta 3361 atggagattt ggcatcagca accattcctg aagaaagcag acttatggcc aaaaaacaat 3421 ctgaatcaga agatactctt ccatccttct cttcctgaag aaactgaagt gtccaacttc 3481 ctctaagtat tgctatgcaa aagctgctgt aattaaacta ttgttatagg gagtagtttt 3541 ttcccttagg actctgcact ttatagaatg ttgtaaaaca gacaaacaag aaaacaaacc 3601 acatactttt gaagtgtatt ttatctttat atagtttgtt tgcaagagta ttttcctaat 3661 aacttcacag tatgaatgtg catctttttt ttttgaacaa atgatggtgt aacattttga 3721 catccataag gacaaatgta gatatttttc ttaaaaactc tgaggggact gacagcatgg 3781 tcagggtgta ttgtagctta taaacatgaa atcttataag gtttcagttt gacagaagtg 3841 tgatatatgt aacttgtgcc atggaccaaa tggtcacttt accacagcta aaaatgagtt 3901 acgatagcag cttgatggtg attgtattgt attcctttaa tcaaaaagga aacacaatat 3961 tctaagtatc tttagcccaa ataccatgac atattgagca tctttaaata accagactgt 4021 attgtccttc atatgtgaag ttgacactac tgatttgtca ataccaaatt ttgggttaaa 4081 gtgtttaatt tttatgtatt tattttcttg ttgcctcaaa agatgattgc attctaactt 4141 ttgtgaccta ccaaatttaa gatgtgtata cgttgttctt tacgttgttc tagaaaagag 4201 attttaatgc tgtagtgact ttgctcactt acactagaga aataaacaac tttcaatgga 4261 agaRaatttt agtgcttttt ttttcctaaa atagatatta agctgctgtt gtaaagtatt 4321 gtttgcagct ctttccaata tctagagaca tttttattta tgaatattta tacaaaaagg 4381 aattctgtca agatgactgc tctatatcac ttgagaatgg cattatttaa ttaaagaaca 4441 aatagcattt tttggtagtg cctgtccata cctattgtca ttgtttgcct tgtaatctgt 4501 ttttttgaat tcattttggg ctgatagttt tgtttaaggt tttggataag gagcacttta 4561 aaacaaactg gtgtgttgtt tttaagttaa tcatatgttt aataaatgcg tggtttttgc 4621 attcaaaMac atcatataat acattgtatt ttttacattc attgatattc tgtctaatct 4681 ttattaggca ctaatataat tctaatggat ttgagtttgt ttgtatattt aggcctgctg 4741 gtgaagacac aaatgaagca taatttttgt tgcctgtgag cttagaatgg tgtgacctat 4801 ttcattttat ttcaatttat cttgtaagtt tatgtgggat attttaaaat aattaatatt 4861 tggtatttgt ttaaaaaact gtaattatag gccttccatc ttaagtttaa gatggacata 4921 aacagtctat acattaatat gtgtttgggt aaactgtatg ctacttgaag tgtttgtaat 4981 tttaaataac acacaggtca tcagactact ctatattcag tggagtcttt gcctgctcat 5041 aagttgtact tcatatgtct aatttgaaaa aaaaagtttg ataatgtagt aagttatgac 5101 gttgagcagg taatcaggtt tttcttgttt tttttttttt tttaaatttt gtagtaagaa 5161 cttgcacagt attaagacca tgaaggtcag ggtgctattg attgggagtt ttttctggta 5221 gtgagttttt ttgcagtaat aggtttataa ggaaaacacc gtaacaagct tttgaactta 5281 tagaaaataa tcacatccat tttctattgc tttacccaaa tagatgggtt cgtgaagagt 5341 tttctgtgaa tattttaaaa atacaatgca attatttctg gtttgtatca ttttttcccc 5401 cttaaaacag gtccttgaac aggtttgcgg tatccctaag tatgtctttt gttatgactt 5461 ggagccactt tttgttgtta ttgttaggaa ccaaacacat ctgtgaaatc attttttttg 5521 tgggagtata aatatatctt gaattatatc caggttttga tcagtgaatt agtagcatga 5581 agaaacctat aaagcttaag ggaaccttgc attttgagga gtcctttatt ttcaagtgtg 5641 cctgactttt ttgaatttta agaaaaagat gaaccatata acaattctgt aagaatttta 5701 gcttgtcaag ttgttctata ctttatagaa aaattattgg ttattattag ttattagtag 5761 tccctttttt ttgctttgtc taggtcatgg ggttattgag aaacatgaaa agttattgag 5821 aaatttggag gttctcttgg cagcctatca gtgatcttct aagcaaaagc agtgcttgtt 5881 tttcatttca agtgcttgct cactattctt tgggaatcgc aggcctccat gactaaaggg 5941 attttgatac aattctttta taaatgagct gaatttgaat tttcttacat catatgggat 6001 tattgcatga tcttcagtca ggaaaaatcc agaaatggat gctattgctt aaacttaaat 6061 tccaaggcag aagatagagt atcttcattt cca'cagatgc cctagaacct ttgaccttga 6121 attgtagttt ttcagtatgt attatcacat ttagaaatgt aaaaatcaca agatctcagg 6181 aaatgcgccc ttgtgatatg ttaataccag attgctgttt acactaattc catatacatg 6241 ataataccac taatttggac tctgctagtt cttttattta ataaggcacc tctaggtctg 6301 tactttgagc cttgatctgg gtacttggta cagagggagt gtcctggcct gaaagaaagc 6361 cagattaggc tgcaggagtt cctttgagga ggtctgtctt ttaataaccc tggcgggtaa 6421 atgaatggta ggtagctggt aggtaggcct tcttgttcat gtctttctgt ctccccaatc 6481 cactctttga attcatgttg aattcttaac ttgaaattct gttgtttttt ttttaatttt 6541 agctttcttt ttattaaact agttaatgta catagtttga aaaaatcaaa tagaactaaa 6601 aggtttatag tgaaaaagtg tagttccctc ttccccagtt tagcattcca aagacaaaca 6661 cttggaactc ttagtggttt gtgttagtat ttaccttcct gtttgaaaat gttatactac 6721 tttttgttga cttatcaatt tcagaaacag tttctcgaat accttttatg gtagacagga 6781 tttactttac ttaaactctc cacctcccac atttttcttc ccccaYccct cccaaaatag 6841 atttatattc acacaacttt tagttagaac aaactttttg tgaaatagat agacataaat 6901 gtagttcact gatgtaccac atggttaact atcattattt cctttttact agaatgttat 6961 tttttctggg gttggtattg cctgattttc aaatttgctt agtttttaat gtacctgtca 7021 caatgactat ccaaaggctt aaatgctgaa aaacatcaga taatcgtatc aattctctta 7081 aaaaagtttt taaaaatat't ttgtcatcct ttagggacat ttcttccaca gtccttcatc 7141 ttctgccatt tgtactgttg ccctccaggc ctgttctatg gttcagcagt cattctgaaa 7201 ctccctttag tttttagtct cacattaggt tttttttttt tttttttttt tttttttttt 7261 tttttggcaa agctcctcca ccagtaactt cctaagaaaa ggtgcataga aagtaaagat 7321 tttaaggctt tacaggtctg aaaatgtctc tactcttgat tggtagtttg gagtctggaa 7381 aataacatgc cttcagaatt ttaagggcaa catttcattg tcttaaggca tttagttttt 7441 ctgtgagaat tctgataccg ttcctttacc tgggatctgt ttttacactc tgtaagcttt 7501 taggaacttc tcattacttc tgatgtttca taataatatg tgggtcttta ttcattcatc 7561 ttgatggcta cttgacttgg tggaatcttt caatttagag atttgtctgc ttcagttctg 7621 ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg atctttttga cagttttctc 7681 tcttccattt tctttgttct atctttctag aatttctgtt atttgaatgt tggacctccc 7741 agagttggac cgcctctaaa tttaaaaagc tttttctctc tagttgttcc ttaagttata 7801 ttatctttgt tctttttgtt atcctccagc tcttctgttg aattttcttt atatatatat 7861 tttaatttct aagaggtctt ttttttctgt ttcatttcat aaaaacctat ttttatttta 7921 tagattaact gtttccccat atctccctga ggataaaatt aagatttttt tcttacatct 7981 ttcctgtttc ttgaatggtt ctccccctcc cccaccatgt ttctgctttc tatttatttg 8041 ttttgggctc tttcattctg aagcctttct tagaaatcat ctattcatat tagtcatgta 8101 ttaaaaagct aatgtcatag gtctttatat gtgggcaggg tttataagtt gtctaaaggg 8161 ccatcaggta gagttggcct tttctgttga ggtcccctaa atttcagtgg aggtcttctc 8221 tttggggcca gtttctctag aaaactgggt cctgatagta gggtaagaaa agacagctga 8281 ggaccccaag atttgtatga tgacttttac ttaatccctc tatcttcatc ctctctcatg 8341 cccatccggc cccacccccc aaatcctcct ctggctgctg accctcaggt tcagtttttc 8401 ctgtcagtgg aggttgaggg tggggcgtag gctggctgtg gagagtaggg gagaggacct 8461 gggttccagt tgctctttat acagacttct agtcctgatt tcagccctat acctcactct 8521 catcttctac aatacctagt gcttccaagt cctgaactta gtagaggttt ggtggggtgt 8581 gtttttgggc ttctcccttt tgggtactta ggctttaact tcctccactc tgctaggtta 8641 ggtattgcac ttcctcctgg tttttatctg tataaacttt gttaaaatct catggctttg 8701 tcgtctgtgc ttttttgtgg attaatacta tttatattcc tttaatatct tttggtggtt 8761 tggaggtaga ggaaataaat tcatgtggtt ggtttgtatg tttcactgaa agtctagatt 8821 taaattctaa tagtgtataa aaaataaaaa ttctttagta ttaatttgta attcattcta 8881 gtgattttta attcatatgg ttttactgta ctacctacct catagggttg tgaggattag 8941 aagaatcaat tcataaaaag cagttagaaa agtgctgggc acgtagtgtg ttgaataagt 9001 gttagttatt aatattgttt actatcatta ttattattac tactatcagt tttttttttt 9061 ttttctgaga cagggtctca ctctttcacc cagtcttgag tatagtggag tgatctcagc 9121 tcactgcagt ctcgacctcc caggctcaaa tgatcctttt acctcagcct cccgaataac 9181 taggactaca ggcatgcacc actactcctg gctaattttt gtattttttt gtagcagcag 9241 ggttttgcca tgctgttcag actgatctca aactcctggg ctcaagggat ctgcctgcct 9301 tgactttcca aaattctcgg attacaggca tgagccacac tgcactctcc tatctctctc 9361 aattcagtaa cctgtttatt cttgagtgaa aatgtttcac cataggaaga attactaata 9421 tatgggaaaa tctagtgttt gataatgttg accctacatt ttttttggac agtctctgtc 9481 gcccaggccg gagtgcagtg gcgcgatctt ggctcactgc aacttccact tcctgggttc 9541 aagcaattct cttgtctcag cctcctaggc tacaggctag gactacaggt gctcgccacc 9601 atgcccagct aatttttgta tttttaatag agatggggtt tcatcatatt agtcaggctg
9661 gtcttgaact cctgacctca ggtcatctac ctgccttggc ctcccaaagt gtcgggatta
9721 cagatgtgag ccaccgtgcc cagccgaccc tgcatttaac tagacttcat atgaaatgtt
9781 caggtgttag gtgttatctt tctaagaaac attcaagcct taagaaatcc aatcagttat
9841 agttacttta aaagttttaa aaagagtata acaattttga tgtgctttta agctttattg
9901 gtattatatt ttgtttttat catataaata tgaagtatag gagataagat aatgcagatg
9961 atgtttgtta tgccttacct accgccagta gatgtaatac tcttaataca tggagtacag
10021 aaatagtaga ttc'ccttcat tcccagtgca acagtgttgg gaggtggggc ctaccaggag
10081 gtgtttaggt catgtgggta ccaccctcat gaatagatta atgccgccgt gtaaagggct
10141 tgtgggcgtg ggtgaacact ctcctgctct tctgccaaat gtctctccct tccaaaggac
10201 gtagtgctct acatagtgtt ggaagcgaga gactgagccc taacatgcca gtgccttatc
10261 ttggatttot cagcctccaa aactgtgaaa aaataaattt tcattcttta tggtaaattc
10321 ccatcactgt atccataacc tcttgtaaat agttgtacac ctttcagaaa atgttgtgga
10381 cttacataag caaaatgcct gcatttattt cataggtaaa attttaatct attttctaat
10441 caaagtgttt tggggaacat ttaatttaaa tactatgttc aattttttta atggtctgct
10501 tcagattttt tttataagtt aatgttttaa aacaaaataa ttgtgttcta ggtagaagct
10561 actctgagtt taggttttta aatctctttc agatatttta agtatctttt ttgtgtctgg
10621 attcagagag gtatgataat gagattctct tctagagggg aagtgggagt aggtaaccat
10681 actactcttt ctacggctac agagccgcat actcgaagga gggagatttt tatttgtaag
10741 gacatttgta ttatctttca ttaaagtaga gtcccatggg cgtggcaaat ggctgtaaat
10801 aaagtaaaag tggctaaaga aaacagtgcc tgctattagc ttgatgctgg cattttcttc
10861 tttccctgga tgtgactgtt attgtcttta ttgggtcccc ttattatctg ctttgatatc
10921 catctctgga actttgatct ctaatttttt tttttttttt ttgagacaga gtctcactct
10981 gtagcccaag ttagagtgca gtgacacgat cttggctcac tgcaacctcc acctccgggg
11041 ' ctcaagcaat tctcgtgact caacatccca aatagctggg actgtaggca cgcaccacca
11101 tgcccagctg atgttttgta tttttacaaa aatttttcac catgttggcc agggtggtct
11161 caaactccta agctcaggag atccgcctgc ctcagccttt gaaagtgctg ggattacagg
11221 cgggagccac cgtgcccgac ctgacctctc catctttggc gtccttatgc tgcctcttct
11281 ccaaatacat gttggccagg gtggtctcaa actcctaagc tcaggagatc cgcctgcctc
11341 agcctctgaa agtgctggga ttacaggcgg gagccaccgt gcctgacctg acctctccat
11401 ctttggcgtc cttatgctgc ctcttctcca aatacagtaa ctaatctagt atattttcat
11461 aggatagaat ttgccctttc tgtcccataa tatgttcctc ttccccaaaa agttttttgt
11521 tggtctcttt ggtaataaaa catttagagc atttactgtg ggccaggtgc tttacctcat
11581 gttagcctta taacagagat gcggaagtac aggactacca gtgcagccaa gatgcacagc
11641 accaggatta actcatgaga ctgattagaa tgatgaatat cttattgtat cctgaaacat
11701 taagccttta agttatccct ttatttagta tttaaattca catgcttctt gatttttttg
11761 gactagcatt agttgttgaa taaataaata attcagaatg tatccacaaa acctagaata
11821 tatgacaggg tggattcagt tttgcttagt tactgtagat aaaccaaata aattaatgta
11881 aagggatatt aatgcattaa gcaatttttt ttgacataaa tgaagggttg tttccatggg
11941 taatatattc agaatattaa agatgagtaa aatgtatgac tataatgaat tccttcttca
12001 cttttactct ggcaaggtgg gaggagggtg ctgttgataa tctctgtgct tttgcaagtt
12061 tggcacaaag acttttactc acttaaatct caatataaag tatattgcca gatctggcag
12121 catggggaca attaggaaca aaggagagag aatagaatca gccatcagga gctataacca
12181 cactcttctt tctcaacctt ttctggtatt agtaggcaga actccttttg gatgcggtga
12241 agcattttat gagtgaacaa tggtgagtca tgtaatgaaa ggagatgttc taggaaaact
12301 tttcagtccc gctcagtgct gccatctttt tctcccagca ctctggactt aaaattctga
12361 gagaacaggg tatttcccac ccttcctctt actttttgaa agcaatacct gagttattaa
12421 gcaatcattt agacagtatg aataaaccaa aattcacctt gacactccat cccctagagg
12481 taaccattct tttctagtat atattcttcc agaccttttt cctgtgtatt gacttttaaa
12541 tagatgtaca ttgcaatagt ttggattagc tcatccatag cagggtggtt aaataaacag
12601 tggcactttt ttttttttga gacagagtct caccctgtcg cccaggctgg agtgcaatgg
12661 cgcgatctcg gctcactgca acctttgctt tccaggttca agcgattctc ctgcctcagc
12721 ctcccaagta gctggaatta caggcgagca ccaccatgcc tggctaattt ttgtattttt
12781 ggtagagacg gggttttacc gtgttggtca ggctggtctc gaatgcctga ccttgtgatc
12841 tgcctgcctt ggcctcccag tgtcaggatc ctggccccag tggcacattt ttatgatgca
12901 tctggtacta aagaaggagc tggctttatg acctttactt gagtattttt ttgtgtgtga
12961 cagttgaaca attctgaccc ctattattgt tggctggaca ggcattttct ttttaattcc
13021 tctatgcaag gaactgtgga ttgctttttt ctggatagct agaacgaggc cattttcttt
13081 caaaagacag tcctagagtt tccagtggtt ttctttaatt gtgtatgcgt gagtgtgaca
13141 gtgtgtgtgc ctgtgtctct atctgtgcat gcatctgtat gcacagtttc ttagttgtgg
13201 acaatgcaac ttggaatcat tttagttttc caattgattt taaaagttta tctatagaag
13261 atgagacaaa tttgctgtag aagttaccac tacttggtca aaatagagag ttgtgggttt
13321 ttttttttgt ttgacaacaa tcaaaagtat ggcctacaga tagtaagggt taaccaccca
13381 gatttttgtc agataatata tacatgtcca tgtaattaaa agtcttcatt tgacaaattt
13441 atatggtaaa tgagcataaa atgaatatct tttcagtatt agaacatttc tgtttatgcc
13501 ctattccagt ggtatttgtg gtcgctaggg gttgctgggt tatccaagtg attcagcatg 13561 ccttcagaat tggcatcctc gggtgattgt ccatagcctc ttgcaatggt gcctcatctt 13621 ggggaggctt tagaacacag ctctcattta gcttattatt gatttttttt tcttgttttg 13681 cagttgttgg ctagagaaaa gtgtgtagca ataagtacgc acacttactg tggtttacca 13741 tcatgaccat ggatagcaaa ctgccttttt tttttttttt aactcctgtg tcatctctga 13801 tgttgtggct aattttaaga ctttgatttt tcagctagga ttagcggaga atgacctacc 13861 tacttgatta atgatgtctg catgggctta ctaagagggt gtggggaatg gtggcagggg 13921 taactatcat cgagaatata gggcaatggg tatattctaa ctttgcataa ctttgctaga 13981 ttatatatat ctttactcac aataagcaaa gttgaacaac ttaaaattga ttcYctgttt 14041 tctgttttca ctatccaact aggatgaact cttaagttca tacatatact ttaaaatggg 14101 aacaaacata gcctgtcatg tctttattta ggctaagtgc atatgtatgt ataagtaatg 14161 tgtatatcct tcacttatta atagtgcctt catgaattca aagacccttg tcacatggtg 14221 ctcaggaaaa aaaaagtgcc aatattcttt tttttttttt ctctaagaag tactagttta 14281 ggtaagtgaa attcaatagc atatcagtgc tttaccttag tcactttttg tgttttgatc 14341 acccttagtc atcactggag agaagagttt gaattcattt gtggttttgc tttagtggca 14401 aacctcctta cagggatgag ttgccctcca ttctccttgc catatttggt taatctaggc 14461 catgaccttt gaggggaaaa ggcacagatt gcaacttttc tttcctgctt ttgtgaatca 14521 ggccatgttc acgcagggtc cttgaaggtg acttgagtga ggaagctata taccgatgac 14581 ttaccaattt ttggtcttat aacattctct ctcttttttt tttttttttt ttgtagcata 14641 tggcttcctc tactgtatgt tggcatttgc cacttttggg gatttgactc ctgcaggtat 14701 tttttttttt ttcttttggg ggagtgtgac ggcagtggat atagtcttag atgatgtttt 14761 taatagttca agtccattag tgtcttactc tagagaaaac gaaagactca ctgtgtgagt 14821 tttcttgccc tctggttttc agaattctgt gtagtttctc aacatttcct ttctacctgt 14881 cctgaagagc tcttatggaa atcatggttg aatgttagta aatttctaag agctttccaa 14941 tgttgtctat aattagccaa aaaaagacat atgaactttt ctcattctgt ctaccttttt 15001 aaaat'tttat tcactttatc tttttctcca gttttgatag atgattatag agttccccaa 15061 aatggccttc ttactatttc tatattaggt atattatttc agaaaagaaa ataatcactt 15121 gggaatggcg gtgagttcag ggggtctgtt aaccatttaa tttaggattg tttatacatt 15181 tatttacctc ttttaaaaaa tttcttcagt taatattcat tgaccactca ctaagtgtta 15241 tgttgctagt atttatcttt gctttctaat tatttttttg taattttttt gctttctaat 15301 tcttgagtga atttaaaact cactgcttta ttatttaata tcttagatac aatgactatc 15361 ttaattataa ttggctttcc aaaattttca ctttttttca gttaactagt tgaatagtat 15421 ttatcattca catttgcacc ctaactattg ttttaaccta aacataaata agcaagcatt 15481 agaaagtagc ataattgctg ttaaaatagt cttttctttg cgtgtttaaa tattgttaga 15541 aacataagca catgaatttt tttggccttt taaatgtctt tattgtagct agttataaga 15601 tgtatatata gcttatatat gtgtatatac acatacatat atatatatac atacatatat 15661 atacataaaa tgattcttgc aaaagtagtt atgggtattt aagtgaaaaa tttgtaagtt 15721 tagaagttaa gaaattggaa ataatggaat tggtttaaaa agctagtctc taaattaact 15781 tagaaaccta atagcaaata cagattttga ttgctataac taaacacttt aattttgaaa 15841 aagtttcaga atagaatttc tattcccagg ggaaaataaa cttcatttaa aaatatatgt 15901 atacttattt tgtgagtatt tttttctttt ccaaattcca aaaggtgtat actctatatt 15961 tttgtcataa aaattctgct tgtcgaaaat attgtagtaa ttttataaat tcaataaaac 16021 ctgagtgatg tgaacagtgt gggggaaaat gtttaataaa tttgccatgc cactgagaaa 16081 aacttaaata cagcatgttt tgaaagtttg aggaagcgta acactttgct ttttaaaaac 16141 gtgactggtt tgctgcacat ttaaatttaa aagctggttg gctattttaa gattagtgct 16201 gtgatgtgag aaaagatagt agtgatgata gctaaccttt aaacgaaagc atacagtgca 16261 tcaccttatt tgtttagatc aggagttctc catcttggct gcacattaca aacccccagg 16321 gtgcagccag gcacagtgat tcacatctgt aatcccagca ctttgggagg cagagttggg 16381 cggattgctt gagctcagga gttcaaaacc agcctgggca acatagtgaa acctcatgtc 16441 tccaaaaaaa aaaaaaaaaa aaaaaaagag ctggacgtgg acgtggtgtt gcttgcctgt 16501 agttccagct actcgggagg ctgagatgtg gggattgctt gagcccgtga ggttgagggt 16561 gcagtgaact gtaattgcac cactgcactc cggcctgggc aacagagtga gaccctgtct 16621 caaaaaaaaa aaaagaactc ccgccccgca gggtgcattt aaatttccta ggttcatggg 16681 taggttgacg cagaccaatt atagcaacac ctctgggggc aaggtgagac ccaatcatca 16741 gtattttttg aaagtttttc agtgattcca atgggtagcc agctttgcaa accattgagc 16801 tggatggaaa ttctcaattc tgattgttgc attttagaag aggtggggga tgtgtgtttc 16861 tatgtgtata tagatagata tagatctatc tcttttatat atctgtctca ctaaagtttg 16921 tggccatcgt ctagcagtca gaaaggccaa ataggtcctt attagcatgg gccgaggttc 16981 ccttgggggt tgttagaaat gcagaatctc agcatcccca tcccagatct gctgaactag 17041 attctgcgtt ttcaaaagtt ccttcggtga ttgctacaca ctttaaagtt tgagaagcac 17101 tactggaggt gatattaggg taaatgtaga tctccataca ccctgccttt tgcttctctc 17161 tggttttctg actaaagttt tgacattttt atgtggggtt ggaaaatgaa gctagtacta 17221 tgtgctcaca tcagcagatt aatagctgtg gtgagtttct gatctggagc agatgtgaag 17281 gagacgactg ccatcaccac aaaactccac tcttccctca gatggggaaa gagaaaacag 17341 ctcaaagaat ggtcactggg ctgaaggcag tgctctttca gtctcttctc tcaactaccc 17401 tgtgtgttaa ggaaaaataa gaagtacagt acttgcaagg atactgcttt atacatgcct 17461 ttgagaattc tctggaagag aaagctactt ccctttaagt aagaaatgta ctcatcccag 17521 tgtcaaactg caattaataa agccaaatcc tccaagttct ctggaataat ttctatacaa
17581 tgccctgcac tcagaaacct ttcttatagt ggctacaatc ccgtatctta aatatttcaa
17641 gaactctcag tctgcttccc tctgtctcaa cccctaccaa gaaataggca gattattttg
17701 ctaggatgct ttcttcctgc aatgattgaa cgattctttt gtgtgaatac tatagggtcc
17761 agtagtgtag tttctgggaa gctctcccat tgctgtccct tcacgtgaca cgctgggtca
17821 ggtaagcacc ctatttcctg actatatttc agatgccagt taagggttca gcatctctgg
17881 cctgagcttt ctgagaagag gaattcccta cactagaaat gaaggacttt ttttacagga
17941 agcaacctga tggcattttg ctgttgaccc tgccctggaa ctacaatgat tctcaaagtg
18001 gaaaagagcc ctcttctccc taatcaagca ctctcttgcc tgctcaccct ccagcaggtt
18061 tggttcttgg tgttttgaag gggtggaaag acaacactga taaacagaga cctggcctct
18121 agttccagct ctgttgctaa ccatgtgact ctgggcatgt tattcaaaga cctggatctc
18181 aatattctcc attttgagct ttctttctgg ggtctcattc tcttcccctt agtggtcctt
18241 taggttttta ggacagagca tctcagcctc ccacctggtc ttggcctcat gggcctggtg
18301 gtgtcccttc tctttggctt ctttgggttg tggcagggat atggaagtga aggggaaaca
18361 gctggcctgc cttatgcatg tgggccgaat ttcttggcaa gttggagaac gaggaatacc
18421 tgtgtccctc atctgaccaa aagctaaaag ttgattatgg tggtctcaga agtggcagct
18481 gacaacaaaa agctgcattc attcatgtgc tacatccctt cataaggctg agaccggtgt
18541 ctgacagagg cccctcaaca tcgctggaga cttctataac tcagggaatt cgtatctatt
18601 gcatatagca agaagtattc atccttgagg ctctgctggt tcaggaacca caaggttcat
18661 tggcagcagc ctctcctgga tgcagaagga aaggaaattc tgagcatcga gcctttgtag
18721 ggtttcaaaa atggagttca tcactgcctc tttttagggg gaactatgtg taggtatttc
18781 tcaggtggct taRacatccc aaggaacaga gttggggaaa tactgaccca gagataccaa
18841 aaagcacaaa accaaactct cactttttat aatttcaaat aaaaacccac gggccaatct
18901 attttcacaa atcctatcta tctagtctgc tgcctcgtag acacatgtag gacagcattt
18961 cttaaacttt taaagccact ggcatctttg taaatgaaac ctaaaaaata tacatagaca
19021 cagtctcagg gaaccttgtg gatcactaac aatctcagga taagcatgag tttcatgttt
19081 ggaatcccga cctttaggcc acactatctt ttgcagagaa ttcttacaag attgagaagc
19141 attggcatag ctgcccattc ctcttacctg ctttccatct cttcttaaaa tagagcagga
19201 gtgaatgggt atcatctcca tcggctgtat tcttgacacg gcatcctcag cggaacttgg
19261 Yttgctgggc aggactcttc tacctcttca ctgccaatgc catagcagtg gcctttgtca
19321 cttaaaaggt tttgggcagt aaggtgtcct cctatatcca tcccttgctg ccacttttcc
19381 tatgcctggg aagtctaaca gattggcttc attgacatgc acccccaagt cccatgaaaa
19441 tcttaggaaa gacctttcca cgattgccta taacctccca gggctataat taaaagggac
19501 atcatgggtt agaaatttgg tggtaggtga agtcttcctg tgagtggaat tctataggcg
19561 gaaataagct ctttgatgta gaaagcaaac aaatgtcata gctttctcag aacagctaaa
19621 gacttggtaa aaataaaatt ttatcaagtc tctagttgac ttaaatctct ttagtgattc
19681 ccttacctct tagggaaaag cccaaactct attatatggt atatgaggcc ttctgcaagg
19741 tggccttgcc tcttgcaccc agcccttcaa aggttgttcc actataatct tttttctact
19801 aaatttaaaa aaaattttct ttaaactttt ttggatgtat tgtgccatcc tttgccccta
19861 ggtctctgca ggtgcaaagg tcctctgcct ggaaagtgag gcctgcccct tcttatccca
19921 tattttaggt tgcagctttt gcctgtttgc ccctttctct gactggataa gatgcctcag
19981 caatgtgctc ccaaacactc cagatttctt tgatcatagc acttaacttc atttcttaga
20041 ggatattcag ttgcctcttc aaccaagatc tattcctttc cacatcttca gtgtttccac
20101 ctcatatagc ctgtctcaac ctgcattatg catagcacca atgaaaagca aattctgtct
20161 tcaaataaat ttggggaaca ctgtgtcacc tcctggaaac ataattcata gctcataaaa
20221 gactgagaaa tcctgtatag agaagaaaaa ttacccaaat tctaagttta ttaggctgtg
20281 gaacagtttt tccacaacat cttgaagagc agagtttggg aaacattgac ccagaggtac
20341 caaaatgcat aaaggcaagt tttcatattt tataacctta gaataaaaac ccatgggcca
20401 gtctattttc aaaagtccta cttaagtagc agtcactatt ttctcctatt acaaggaaat
20461 gactttctct acctgaattt ctcaaaatta cgtttggggc agagtaaaca aaactgaaaa
20521 ttactttttc tttctggagc acgatcataa aacaatcttc ttgaatcagt caccatggag
20581 atatcaattt aacctgttat tttatgggct gtttctggtt taacaacctc tatggttcat
20641 cgtttctggt ttaacaacct ctatggttca tctacataat cagctactat tggatagaga
20701 aaaaatctgt cttttcagaa attccctcag aacacttact ataaagaggt gctgttccat
20761 gtccgccaca tctagggtga caggttggat tatgtccctc cctaagagat gttggactca
20821 taacctcaag tacctcagga tgcgaactta tttggtgata ggctttatag aggtaattaa
20881 gttgaagtgg agtaactagg atgagcctca gtagtatgac tggtatccta aaatggagaa
20941 atttggacac agacatgcag gaaaaatgtc gtgtgaacat gaaaatagcc atctacagaa
21001 aaggagagag gcttaggaga gattcttccc tcagagttct cagaaggaac caaccctgcc
21061 aacaccctga ttttggactt ccaacctcca gagctgcgag acaatacatt tctgttgtgt
21121 ctgccactcg atttatggta ctttgttcca gcagtgctag aaaatgaata cactagtccc
21181 ctctgatctg tggtttcact ttctgctgtt tctgttaccc atagtacagt acaataagat
21241 atatagagac agagagatca cattcataaa acttttatta tagtacattg ttatattcta
21301 ttattgttgt taatctctta ctgtgcctaa tttgcctaat ttataaatta aactttttca
21361 taggtatgcg tattagtcca ttttcacgct gctgataaag acatacctga gactgtgtca
21421 tttataaaga aaagaggttt aattgactca cagttccacg tggctgggaa ggcctcacaa 33
21481 tcatggcgaa ggcaaggagg agcaagtcac atcttacgtg gtgtcaggga agagagaaat
21541 gagaaccaaa caaaaggggt ttccccttat aaaaccgtca gatcttgtaa gacttattca
21601 ctaccatgat aacagtatgg gggaaacggc tcccatgatt caattatctc ctaccaggtc
21661 cctctcacaa ggtggaaatt atgcgagcta caattcaaga tgagatttga gtgaggacac
21721 agccaaaccg tatcagcatg tacgtataag aaaaatcatg gccaggcgtg gtggctcacg
21781 cctgtaatcc caacactttg ggaggccgag gtgggcagat cacgaggtca ggagatccag
21841 accatcctgg ctaacaaggt gaaactctgt ctctactaaa aacaaaaaat tagcctggcg
21301 tggtggcagg cgcctgtagt cccagctact tgggaggctg aggccagaga atggcgtgaa
21961 cccgggaggc ggagcttgca gtgagccgag attgcgccac tgcactccag cctgggtgac
22021 gagtgagact ccgtctcaaa aaaaaaaaaa aaaaaaaaaa aggaaaaatc atagtatttg
22081 taggattcag tactatccac agtttcagga ttccactggg ggatcttggg acaggttgcc
22141 caagaataaa ggtggcctgc cgtacatcta atgagtacca gtgagaccta ggctaggtcc
22201 cctctaaagt gctgcttctc tagtccctag cttcaatatt ctgtactgtg gtccctaaca
22261 gaataatgat tgctggtatt tattgggtgc tctgctaagc gctgttctga gttttacaag
22321 tagtaacttg tctaatcgtc acaataatac tgtgaggttg ctgctgttat tctcatttta
22381 tggacgagaa tgtcaaagaa tggagaagtt gggtaacctg ccccaggtga agtaagttag
22441 taaacagcag ttcctagatt tgagcggggc cctcagggtt ttgaatgcag acatgtaatt
22501 gcaacatgat tcggctgatc atgtagccag ggtgtggggt ttataaaata gacgtgaata
22561 aaaaaaaggg gcaggatgac agtgaagctt gctcatgggt tttgtggagg gcgtagattg
22621 gaaccaaaaa gcacgcctcg gcttggtgct ccctgtcgcc ctctgttgac atggggagag
22681 aagagcgggt ctgactcgga aatgacgcca taatgctgag gaaccactcc tggcgttgcg
22741 gaaggtgggg tgtggggccg gaagaccctc cccccgcgtc ttcagaggag cgctgtttct
22801 ggaggagtta ggggaaggag gctgcctggt aagccaggaa aggggcttct ggccgttctg
22861 ttggagcgcc tctgactNac ttcccccatg tgtccgtgtg gccacaccgt gctactttca
22921 gaggcagtgg gcttttcgct aagggataaa aagaagtaaa accttgcctt tagcgcagct
22981 ctccttcact gggcagattg ctctccctcc ccactctccc aggcccaaat tggatctcag
23041 cgcctgggag ctccaacttt agcaagtgcg aaatcctgtc ttcggttttg gaattttcat
23101 ccgcacagac tatatcagcc acggatagga tatgtgagaa taccagcatg tcaaggctgg
23161 gaatcatcct taggcatcac ctagccagtc ctgcctctca ttttaaggta agaataggca
23221 tttaacattg taagatgatg tagtgggttc tgttggcatc acttagatgc ccaggaaatg
23281 tgtattcttc catcgatttc tcgattgttc actgtttggc tgcgttagtc cacggagaat
23341 tgtcctggcc aacaggcacc cctcttcact tgggaaggtt tatttccctc ttccatccct
23401 cctcagcccc tcagcttata gcctgtagcc attgactggt tggtaagggg gtacaaaatg
23461 tcagccccct tgctcctaga tggaaccaac tctgtggtac gattgaagct ccctgtgaga
23521 tctggctgag gcttctgctg agaccacatt cgtgctcagc ttgcttcctc tgccctatct
23581 gctggctgcc ccattccctt tttccctgag agtaataatc cctcaaagaa tttctgagac
23641 aagaatcccc gtctcaggct ctgcttttag ggaatctgac ttaagacgga aaacttgctg
23701 aataaattaa tgaagaccca ggatttttct ctggagaatg aaaactgaca gtacatttgc
23761 ttcagctttg gtcacctgta cctacattaa ggaggtgaat ttaggcaaat aacatcatag
23821 gcttataatt atgattaaca aattgaagtg gacaaataca gatatttttg gcattatttt
23881 attaccaagt ctagatcagt tgtttaaaaa gcatagtaga atgacactat ttaaaatagt
23941 gtcagtcttc catatactgt cattctacca tgcttttaaa acaactgatc tagacttggg
24001 aataaaatag ttctttagtt ctgaggtcct ttatttgttc tgtctcaatt tggagctgca
24061 gtagccaata tggactcaga ttcacaactg atcattttcc ctgaagttgt ccttttccgt
24121 tcactatgct ctttgaggga cagtatctat accgtgttaa tcatctttgt attgttccct
24181 gtggtgttta gcacaaaaga ttgtgaattg tagatgttca aaatccatca aagatattga
24241 atcacagctg attccgagtt gaatcagctc tcctaaaaat atttttcttg atatatttag
24301 aagataaact tttggggata aatatatcca tgagaactat atctaggctt tttaaacaaa
24361 attgctgaat acatctaacg ataatttaaa catttgctta aaagaactta ttagcatggg
24421 ctgttttttt ttatttttta ttttattatt attatacttt aagttttagg gtacatgtgc
24481 acaatgtgca agttagttac atatgtatac atgtgccatg ctggtgtgct gcacccatta
24541 actcgtcatt tagcattagg tatatctcct aatgctatcc ctcccgcctc ccccaacccc
24601 acaacagtcc ccagagtgtg atgttcccct tcctgtgtcc atgtgttctc attgttcagt
24661 tcccacctat gagtgagaat atgcggtgtt tggttttttg ttcttgcgat agtttactgc
24721 gaatgatgat ttccaatttc atccatgtcc ttacaaagga catgaactca tcatttttta
24781 tggctgcata gtattccatg gtgtacatgt gccacacttt cttaatccag tctatcattg
24841 ttggacattt gggttggttc caagtctttg ctattgtgaa tagagctgca ataaacatac
24901 gtgtgcatgt gtgtttatag cagcatgatt tatagtcctt tgggtatata cccagtaatg
24961 ggatggctgg gtcaaatggt atttctagtt ctagatcctt gaggaatctc cacactgact
25021 tccacaatgg ttgaactagt ttacagtccc accaacagtg taaaagtgtt cctatttctc
25081 cacatcttct ccagcacctg ttgtttcctg actttttaat gattgccatt ctaactggtg
25141 tgagatggta tctcattgtg gttttgattt gcatttctct gatggccagt gatgatgagc
25201 attttttctt gtgtattttg gctgcataaa tgtctttttt tgagaagtgt ctgtttgtat
25261 cctttgccca cttgttgatg gggttgtttt tttcttacac gtttgtttga gttcattgta
25321 gattctggat attagccctt tgtcagatga gtagattgca aaaattttct cccattctgt
25381 gggttgcctg ttcattctga tggtagtttc ttttgctgtg cagaagctct ttagtttaat 25441 tagatcagca tgggctgttt tgaacttgag ttgccttatc tatctatgat cgtttttgtg
25501 atacacgaag atgcatactt ccccacccca gaatttcaca gaaaacaacc agccacacat
25561 tttgaggtgc agatagcttg ctttattttg ttgttactat ctcaaggagg tccaacaatt
25621 ataactaaca attgaattta tacttgcatg aaaagaacta catcaaattg acattttggg
25681 caattagtaa tattgtttaa aatttaacaa cagctttatt ttgttgttgt tctttacttt
25741 ttgctgtggc tcattgctta ggtgcccagg tttttcaggt gcaattaaaa tttagaacta
25801 ccacacaaag gcattggctg cactctggga cctccaagag ttggcactgc tctggcatag
25861 gaatacttga atagcttggt taaatgaagg gatggccagg agatgttact tttccaagct
25921 attcaggcac tccttatgtc agagacagcg taaaatgagc gaacaaagtg ttggccgtta
25981 cactttgctc tggtaatatt tgtcatgata attttgtgtg acatcatcag agaaaatgta
26041 agacgttaaa gtgttgctta caggagggta aatgcaaaca tcaccaatgc cttacatttc
26101 tgattcagta ggtattggtg cacatatcaa gtgatatgca cagcatcatt ctcttttagg
26161 tggacaggac atcttcaatg gggattttct caacccactg aggcatactt agacatcaac
26221 agtatgttga ggcacaaaga ttgattaaat gttgagcagg gtattcgttc cataagaaac
26281 tttctcctac caacagttag tttcaaaact tacattgtaa aatgtcataa aataaatggt
26341 acaaaacttt ataaccgtta atccggctat atacagtatg tacatgtcac tagcaaatgt
26401 attatattct taggtcaaca attatgaaaa gattgaaatg aatatttttg aactttgact
26461 catttaggtt gatacaaaac tacttttttt tctttaaata tttacagttc agaaagatta
26521 gtccgtgtga aacgtttgcg aaaagttaag aatgagtgta gagtataagg gcacaggccc
26581 tcttaaatgg atcgaatatg tatattcaca gtggcagagt cagatccaaa ttcattccct
26641 aaactcaggg tataaagtcc aocatcttgt ttctgtacgt ccatgatgat cagggttgtc
26701 aggtcatctg tgttttcaat gtggaacctc ccctgttctt gactgtggat ttttcttcca
26761 ccacaggacc atgttacttc tggggtaggc tcacccgtga aagcacaggc tactgttaga
26821 actttgcctt catcaatgct gatatcagat ggaagagctt caattttagg cggaattcct
26881 ttatggaaca atgacaaaaa aaaggtctta gaatcactga aaaaaataaa gatttgcttt
26941 ctactgaatt gtgtgtacta aagactacac catgttactt ggcttgtcta cctctaccag
27001 taattttatt gctcacctct tatgcctgct ttaagcattt tactagttga gctttccagt
27061 tgtgtcatat tagatattcc cataaagctg ctggaactca tttctacaaa ggactcttgc
27121 atggaggaca tgctttgggc agacatgctt gcaaatttca tctcagtcat gctgctagca
27181 ctgctgctgc tgaaactgct gaaggaggcc tcctgcttgg aggcagacat ttggactgac
27241 tgagacgaga agcttccttg caagcttgtg tcaccacttg ttctcaatac tacctctctg
27301 gaaggttctt caactagagc tgtggagcat agcagataca cagtgaacat caatgacatc
27361 tggttaattg atgacatatg acgctgactt acagaaatgg aaacttcaac acacacacag
27421 cagcataacc caaacaattt gctgttgaag cataagcaaa tacatatata catacatttg
27481 tcaagagcta gctcaatgtc tccaaaacat gaatccttat atttctatca ttacattaca
27541 tgcaggtgct aggaatcact aggagaaaaa tgattagtca tatagaaagt aattgacata
27601 ttgtgcatcc tgcaaataaa tgttacacgg cagttggtag ctttactttg aaatgtaatc
27661 tatccaaRtt tctgtgtagc tataaagaaa gtttgactac aagaatgaat tcacatcagt
27721 tggctgtccc tctttcacat ggaatttaat aaggcttctt agagttttct gtgcttgaaa
27781 gagaggcaaa aaaaYgatct aaaggcctta aacatgtaag gaagaagggt gaggagatac
27841 ttacgaagga ccattaagga agctgtagct gaacactggc cacggaaatt tttggcctta
27901 attgtgtact ttccactgtc gctgactgat gcatttcgga tttcaaggga gtatacattt
27961 ctggagcggc ttatgctgac atttgaagaa atagaaatct aagacaaagg aaaaagaaaa
28021 gaaatgttga agttcttcag atgtggaaga catggtattt tagtttttct tcaataatat
28081 attcataatt aaacttactg gcaggttgtt tttaaaccat tcgatttcag gggatggctc
28141 gccactgatt tcacaagtaa agagaacatt ttgtccttca ttaatatttt gagatctagg
28201 ctgtgagatg aaggctggag catctgagag ttctttgctc agtgtcaaat catactgaca
28261 cttgacgatt ccttccttgg ttttgcttat gcaggtgagg attccttcat ctctgtgact
28321 ggcttgcttg atggttaggg tctgatcgct gcctgagaca ccatatcggt actcctcaga
28381 gttggtaagc tctacgccat tcagtaccca tttcacatca gtggcaccag caatgttggc
28441 ttttaaaacc agtctttgac cttcgtttat gctcatctga gtagaaaatg ctttaatctc
28501 agcatgagtt ctgacttctt ctgatgcctg tgatgtttta gtgatttcct catggacaat
28561 ggatttttcc agggaggttg ctgctgattt cttgacttct tcagaaatca gaacctttga
28621 agcttcctct ttgagggcta acttttcttg agattttgcc tctgacatct taatttcttc
28681 agaccttagg gctttttggg aaatttcctc ttggacaaca gctttcttct gaggtgtaat
28741 ttcagaagtc ttttgtgtag agactttctg tgcctcagta tcttttatag ctaaaaaaga
28801 aacctctgta aggcaaactt aattagaaag agacccccac cttttactct tctagattct
28861 gcgtgtgaaa aacataaaaa gaaaaaaagt caacttcttc atgcaatgta ggtaatacct
28921 aatactttct cagttccact tttggaaaat tatcatgtgt gacttttgag atcactgtaa
28981 tgattcctta aactaaactg agccaaagat gattcatatg acttaaggaa aaaagtcttg
29041 aaaagaatag tcaacggatt taggagataa gtggatttgg gagattttga gtaaccaaaa
29101 cattaaatta tctttcagaa gattgaaatg aaagcaaact aattttaatt ggtaaaatag
29161 aaatagttct ttaattaact ttgcactgct ttggaaaatt agcaactgtg aaggtttagg
29221 caagtagtgc cattttgtat attcactgac tttattctta aatattcctg ggagcagggg
29281 ctgacactat ggaatcttat ataataccac atataactag gtacttaata actgcttttt
29341 atgctcatag aaaagcatat gctaacttca aatgtgtgtt tctgctttgg tgtacaaatt 29401 gtattctgga attaggaaaa taattttatt ttaatacttt cttgtatgtg tatataaggg
29461 gggatgtttg aaaatatttc cctaatatta tcttctgaag aaatgtgggt aaaaacaaaa
29521 gccaacctac cttttattgt taatttgcag ctagaggaca cagatccagc tgaatttttt
29581 actgtacaag tataaagtcc actgtcagaa gtatcagtct tatgaatttc taagaagaac
29641 cctcccttgt cttcagagag tttatattta cctccttgtg taatggcctg tagaatgcaa
29701 atgatttgtg ttttaaaaag tgatttcatt ttaagctttt tttgggaagc tgaatttaga
29761 agataaaata ttttagaaga taaaagaaag agacatttaa gaactggtta cctttccatc
29821 ttttgtccag atggcagttg gccggggttc tccagtagcc ttaactgcaa atttagcaac
29881 actgtctgaa gaaacagttg tatcctgcaa cccagtaaca attactggtt ttgaggaaat
29941 tggttcagga gcttttggtt cagtttttct ggttactgtt gactcagtgg ttttctgatc
30001 tgatttctta gtttctgata tttttgatac cttctcatgg atactcttaa aggcttgccc
30061 cataaattgt aagttggttt tagacgttcc accttcacca gaaatctcac aaacatattc
30121 tccttgatca gattcagtga ggttattgat tttgagctca taggtaccat ctgctgaata
30181 atgaaactgg aaatgcccat tttccttcag tttcttgcca tctttatacc aggtgacctc
30241 ttttgccctt aatacactgc tttcaaccac acaggtcagt tttgcaatct ctttagaagc
30301 ttctgctttc aggaactgag taatctttgg tggggcagag actgggaggt gctgaacttt
30361 ctctSttggt gttggttttg tctctgtggg tgatacggct ttcgggtgag aaggttctgg
30421 agatttcact cgttttggag acttaactgc ttctggggat ttcacccgag gctctgggga
30481 tttgactctt ggtggtgatg tcacagcctt ttcagttacc ctggcctttt gaatagtcag
30541 agtgaactct gcttcttgtt tcccttcact gttttctacc accacgctgt aattgccctc
30601 atcRgaagcc tggactgaag agatctcaaa ggttgatttg tactttgtgg tggtcacttg
30661 gtggcgggca gaagtactta gcacttgtcc tttacgcagc caggtcacag ttggtaccgg
30721 ctcaccatcg gtgtcacaag aaaaccttgc agactcgccc tcgtagacgg tcatggaccg
30781 tggctttgtt agaattcttg cYgccaaagt cgtcttgatc tttctggttg tggatttttc
30841 ttccagtgac ttttcttcta atgcagcact tttcatttct gcagatgcag agtgttcata
30901 tgaggagaga ctttcccttg tctccgtcat ctgtgatttc acttccctga cagaagacga
30961 agcttccatc tcaSatgttt tcttaaatga tgRaacagca tacgcctctg ttcttgtcag
31021 ctcagggaaa acagatctgg ggacctcttc atctctgcgt tgggaagcat aggtggtata
31081 atcccctcct gtcacgtcca acgttgcata gtcagaagct tcgcccttgt agttggtgca
31141 cacagcacgg taggttccac tgtcatcagt atgacagtcc agaatttcca gggtgagRac
31201 tccactcgtg ttggtgtaat gaatcttact gctttcttgg agttccacac cattgtggta
31261 ccatttaacc tcggcagttg gcttagactg aacatttaaa ataaaacgtg tattttggcc
31321 acatggtacc ctgtgcgagc gcattctcag tgtgattcga ggggcatggt ccagtgtgaa
31381 aggctgctga ctcaaaactt catacttcct ttctgatgtc ttctgagttt ttaaagcagc
31441 tttcatggac tcatacctgg aaaagatatc aaatcttgca gacctctcaa atctcgagag
31501 tgatctctca ctagacacag ggctggggga tcgtgggcga gttctctctg gagtaggtga
31561 ccttctttct gtgtcatctt cgtattcctc agccggttgt ggacgtgacc ggatcagctc
31621 agacactggc ctcattaact caatataagt tggagacagg gagcgccgtc gtctcagtag
31681 tctagacgca gatgaggatg attctctttg agcatgtttt gagatttcgt attcttcctc
31741 aatttctgtt atttctgtca cttctctttg tcgccttgat ttctttctag acttttcctc
31801 ctttgacatg aagtcaagtt cgcttttgta ttctgagaga tgctgggtgg tcgtaactgg
31861 gcgaagcaac tcttcatcct ccctctcagc catgattctt tgccgttgct ttggctgtct
31921 gtacgcagcc tgggcatgcc gttccctcag ttctgcataa cttgtactag cttcagcctt
31981 cgttgggatg tgataggttg aatacctgaa gtYttttctt gtttcctcca ccttgacatg
32041 agcttgtggt gaagagtaac gtaggctaga aagctcaaag tgtggagggc ttcgacttgg
32101 gggtgaagct gaaaaaccta actcaagctc ttcttcaaga cgcagcctct cttcctctgt
32161 tcttttcatt gctaagtagt catcaatggg gaggagtaat tcttcatcag agatgtcccc
32221 aagagaacgt cttctaggtc ggtagtaaaa gtcataatca ggagaaggtg tacgccggcg
32281 ggctggtctc actatctcaa gatcatcttg ggacagttta ggaatacgcc atttaggtct
32341 gtattgatct gtaatgcgtg gaagaggcat cacatagaac tgttcccatc ttgaaaggcg
32401 gatgYgcttg ggtcgtttct gtacaactct gtcaagtttc ccaggoattt catactgatc
32461 acgtatcttt ttataccact tcatgtcaga catgggcacg aactgcttga tgcgttggtc
32521 ttcttctatg gtagtctgct tatacttgcg tggctctggt acatcataag gcatccggag
32581 ttttctctcc tccttctttt cttctatctc aagtctgaat tcccctttta cagtcttggt
32641 gcttacagcc ggtttataaa ggacagcagc ttctctcaga gcctctttag ctacctgtgt
32701 cagtggtaca ctttcagttc cagaaagaat ttcagccatt ctgagggttt tgtcaatttg
32761 tttttgtacg tgtcgctcat gctcctcctt actcttgaat tcctgtttct tgtacctcag
32821 gcgttccact tgtaggtgag cctggcagct ggtggaccca gctgtgttag tggctgtgac
32881 tctataataa cccgtgtctt caggcaaagt gtccctgatg tgcagagcat aataatccaa
32941 gccttcatgg ataatttcaa tgttaggccc gagggaoagt ggctgaccat ctttctccca
33001 ttttaatgtt ggtgggggga tgccagacac tctgatctca aagcagacac tttggccttc
33061 ttggcattct gcatttgcca gtaacctttt gaacatgggt cttaaggtac tatctgttgg
33121 aggtgggtgt agRgttactg tcagctttgc tttacagctg tcttcaccat atttgttcct
33181 tgccacaaca gtatattcag cgtcatcatc tRtagtgaca ctgttgattg ttaattggta
33241 aagacccttg tctgactcaa atgtgtactt cttgtcattg tcacctggtt tgattttctg
33301 acctgattta taccatgtta catgaggctc tgggtggaca gttatagtta ctMcaaaccg 33361 gacattttca cctacataag ctgtcttatt atagagaggc agggtaaatt ctggtggcct 33421 ttccaggagt ctcattgtgt ctgttctgcg cttaattttc ttcatggttc tacggcagta 33481 atagtcatag acttctctca cacctttaac aaatagctct gcataagaac tgtcttcacc 33541 atagtcattg actactttgc atctgtaggt accatcatct aatttggtaa tgtctttgac 33601 atagaggatg gccactccat cttcgtaggt gatttcgtat ttctcactgt tctccagctg 33661 tcggacRcca aagtaccaag tcacttgggt agactgatca taattttcaa ttttgcatac 33721 atatttgaca tgtcctcctt cttcaccaac tgcatgcatt atctgcccag aaactgggcc 33781 aatttcaatg gatgccactt taactttagc aacactcact cccttctgag atcgaattgc 33841 accaccacag gagatccggg ctgctgacac aaccatgttg aggtctttct tgatcagggt 33901 gtggtaataa cgccggtgtt ttaatgttct gataacttta gtactgactc tttctatctt 33961 ctgcttcaac catgggtgct ggagagcctc cgatgctgtc atgcgagatt tcctctcttt 34021 cactaacaac cggtcaacaa aatccatggc ttcaatgcta atctctttga atgcttcctc 34081 atcgaaagta tattcagcat tcatgatatt ctcaatgatc tgttggttag tttcagccag 34141 gaatgggttg ataccactca atagcacata taccagtgtt ccaagtgacc acatgtctgt 34201 ggctgtgctg acaacatcat gctggtggac ttcaggtgca tagtattctg gggcagtgaa 34261 tagaagcctg aagttgtccc ctggtttcag ctgacgggct tgaccaaatt ctatgatttt 34321 aatggtagag cttcttctgg tttggtaaat gatattttct ggtctaatgt caaagtgtcc 34381 aatattatga ctgtgtaaaa actgaagtgc ttcacagacc tggtgaacat aacttacaat 34441 ttctctttca ttaagttcaa aagcacttgt gttaatgcgc tcaaatatgt caagtcctga 34501 tataaactca aagatcataa ctaattcttc catgctttca aatgattcat ggaggtgtaa 34561 gatgtttcta tgcctagcaa tattcagaat ggaaatttcc ttctttacca aaacctgatc 34621 agtccctttg actttaacaa atttggccat gtatgtcttc tttgaggatg tttcaacaca 34681 acgatggaca attccaaact caccacgccc aagatcttca gcaatcatat atttctcata 34741 gagttccttg gttgaagagt gagatgcttt agtcatggag acttccctgg tttcatctac 34801 ctcttcatca tagttcatag ctctggtctt atcttctttg gttatggttg gttctgaagg 34861 ctctgaaggc ttgctcagac caaatttatt ttcagctatt acccggaact ggtaacttgt 34921 ttttccaaat aagttgatca cggtataacg tgtttctcgg gcctgtccta cacggagcca 34981 tctttctgca gtagttgcac atttttcaac aatgtagttg gtgattttgc tgccaccatc 35041 agaggctggc tcagtccatg ttaagttgac agaatctcgt gagRcatcac taactttgac 35101 tcctctgggt gggtcaggaa catcagccac atccagttca actgtcttct gatcaattcc 35161 aaatctgttt ttagcacaga ccacatagaa accagcatct tttctctcta ccccattggg 35221 gaaaacaagt gatgtgaagg atcttgtgac aataacttgg tagtggccat tattgtcaat 35281 gagatcttgt cctttctgcc aggtgatcac aggatctggt ttgccactga aaggaatctt 35341 gatgctgacc acttcacctc ggagagcatg aactgctcςc atgccttcaa gagttttagg 35401 taagtgtatc ttagctggaa ctgtatcaga aaaaaaaaaa aaaagaatat aatttagcaa 35461 catctaacag tatgtgttta agtgcaatag cctccacccc caagttaata actcatttag 35521 cctcaacttg atgataattt atttattttt accttccact tccaaggagg cagtgccaga 35581 cacagatccc ccttggttgg tagctctgac ttggtaaact gtggcatcat catctgtgac 35641 acttgcaatg atgagctggt ggtagccacc cttaaattct tgaatcctat attttaatcc 35701 atctgcaatg atttctttgc cttgtctgta ccatttgaYg ataggttttg gatgaccagt 35761 cactttgcag accaaggtag cattgctctg atatctgaca tttagatttc tcagttcctc 35821 tttaaagtgt ggtgcctgaa ttgggacatc agatttggga gtgatgggtt ctgatatttc 35881 actccattca ctttccccac ctagattttc aYatttcaca cgaaactcgt attcaagacc 35941 ttcaataagg tttttcactg aaaagacagt ttctcgaatt tcttctgttg tcacagaaat 36001 ccatttattc tgcttcttct cacgcttctc aaggtagtaa tttctaatct ttgcacctcc 36061 atcactggca ggtggcttcc aggccacaac acaagaatct tttgtgacag cagtaatagt 36121 tggtttgcca ggagcacctg gcttttctat taaacaaaaa aaagatttga gtcatgagat 36181 gaaacaggca gctttattaa agcttatttt tttaaaaaag aatgttatat gagtccaaaa 36241 ttttgttaaa aaatagctat tccagaaaga tgaaattggt tactttatac taagacatat 36301 caaaaaccaa agttctattt ttacaatggg gttaggatat atatttctcc ttttagatat 36361 gcaaacattc aattaaagtg aaagaaaagg cacttgtact ttatctgaat ctttgctatt 36421 tcctttcaaa atttctttct gtttaaaaga attttatgca aagatggaac tttaccatag 36481 gaagatacag aaatcaagtt gtactcacca aatggactct taatgatcac aactgaggac 36541 acttctagag ggtcactgat gccgaaagtg ttctgagctg aaacccggaa gtaatagcca 36601 gcattttctg tgaggttcac aattctacag gttgtcactg agatggctga agacaccaat 36661 tgccattcag ccccctcctt ggcctcacat ttttccacca catagttggt gatccaggag 36721 cctccgtcat ctgcgggtgg tttccagctg atcactgcgg agttcttcaa tagagcttcg 36781 atcacaattg gtcctgtagg tttgtctggt ttatctgttg ggggaaaata caattgtggt 36841 ggttttcata gtgtgttttt gagatttttt tttctttaaa aataaaaagc actgaaaata 36901 aaaataaaat accttgtatt tccacatcaa ggatggcatc aactgttcca aaaacattgc 36961 tgagctggac tttgtatttc ccagcatgag tcttacgttg gacattcttc atgacaagat 37021 gagtatagtg ctcagtgttt tcaatagtaa tgttttctga gttttgcaaa agtttctgac 37081 catggaacca agtcatggca ggtactggac gaccaatgta cataacatga agccgaagtg 37141 tggaacccac agctccataa tatttctctt tcagtgggta accaggatgg aactgcggtg 37201 ttgcttgcag gagaagctta ctactggttt ctacttctcc aacctcattg gtggctatgc 37261 aggtataaac accttcatct tcctgttcct ctgtcattac tgtaagagtg tgtgtgcgtc 37321 catctgaaga cattttgtat ttccggcttt gtatgagctc tttaccaaat ctgtaccatt
37381 taatgtcagg aagaggcctt ccaacaatct ggcatgagag ttgagcagct tcacccaatt
37441 ttgtggtaac atccttcatt tctttgcgta ttcctggggc ctctccagct gaacaatatg
37501 aaagataata ttaagtgact gttRatactc aatctgtaat cctttgtcct tgtatatcag
37561 gaatgaattt accttattta ctgacacata ccatgattta catattagcc taattcttaa
37621 aaactctttg aagtgatttt cctaggatca tatggtaaat agggattcaa tgagcacatc
37681 gtcacatttt atgtagagtg tttttttctc tttatgattg gaaggggatt aataatgtat
37741 aggcttttaa gggactgtaa attctctcta taattctgta gtattcttgc ccttacttag
37801 gttcattgaa gtggcataca attgttgacc tttcaaatct tgttgggaaa tatttttaaa
37861 aagaaaccat tgcattagag ggaattactc aaggaaagtg ctcagttcac tccatctaac
37921 atctgctgag gttgtgtgct tggaatggtt tcctgtagaa cttgtcatat ttcctgtgtg
37981 tgtacttgct tttctttctt aacacttgct ctccaataaa gctttccagg gttctactta
38041 gtatagaggg ggaattagcc ttaacttgtt tagtttgtaa atcataagta gagaaccaaa
38101 ggctacttac atgtgagttt agtctttata gacatagcag ttctgcgagg tctgctcagc
38161 ccagtctcat tctcagcaaa aaccctgaat tcatactcag tagcttccag caaacctcct
38221 attgtgaatt gcttgtcctt aatacgttcc ttattgctct tcttccaggc actgtctcca
38281 gactgtctat attcaaccca gtatccaaga atttctttac caccatcaca ttcaggtttc
38341 tcccactgta gagtgacact atctttggat attgaaagaa tctcaagttc tcctggttgg
38401 cttggtttat ctgaaatatt ttaaaataat gaaaagRgag tcagctttac tggtgaaata
38461 aaaggaccaa acatggcttg cttctttaat ttaacccctt cttctgaatt ccttaccaaa
38521 tggatctttg caaacaactg gttcagaagc agggctggtc tcactcaggc caacatcatt
38581 ctgtgcgatg atgcggaact gatactcagc atcgggaaca agccctgtga cagtgtacat
38641 tgtggtggtg atctgagtct tgttgtgtct gacccacttg tcagtggatg tctctttgcg
38701 ttcgatgtag tagcctgtga ctctagaacc accatcatct ttgggccggg accaggacaa
38761 gctaacagaa ctcttggtta' catcgagtac ttctggagga ttgcttggag gttctggagg
38821 atctgtaaat ataagtggaa agcacacatg tattagaata cagtcccaag tattataagc
38881 caatgacttt catttaaaaa cagaaaagtg ctgaaataat gtttataatt ttgtggttga
38941 aagggcactt actcaatggt gtttttggtg tgactggttc ctcagatttc aagggtttgc
39001 ttatgccaaa ctggttttct gctgaaacac ggaaatggta ttctacattc tctttgaggc
39061 cttttaccac cagagatgta cctcggactc tggaatcaat ggtataccag gcggctttag
39121 gcacttctcg tctctcgagg atgtagccta agatgtcagc accaccatca tcagcaggag
39181 gtctccagct gaccctcaca gagcggactt ggatgtcatc atattccagt ggcccttctg
39241 gactgttggg acttcctatc accctgacct tgatgtagac agccttcttg ccacatttat
39301 tttccagaac caggtcataa gtgccagaat cacccctgtc tgcttctttg atcacaagct
39361 cagtgtgtgt ttcagatgtt gcaatcatgg cacgcttact aatatcctgg ccttccttgg
39421 tccatttaca tattgggaat ggttttcctt tgattggtat ggtaagtctg atgacgccac
39481 cttgccttac aaagatacct tcttggtatc tttcatcaag ttcataatca ggatattctg
39541 gaaaaaaagg tagggtttca atttagcatt tggggtaggg ggactaagaa ataagtctat
39601 ggcaattatt gcagaaagca aatgatcatW tgtctcacca agcatttcag tgactttgac
39661 tgttcctggt acctcagcag gctcaccagg tccaccagca ttacaagcta ggacgcggaa
39721 cctgtattct gcaccctgag gtaggtgtgt tagagtatac tctcgaatct tggttggagt
39781 ggtgttacac ttggtccatt catgttgatc tactttttgc atttcaatca agtagcctgt
39841 gactttagat cctccttcat cttctggaac actccaggcc agggagactg atgtccttgt
39901 tgtatcagta actcttgggt tttgtggctt tcctggagga gctgagaata agaataagaa
39961 tatactggtt aaagttgctg caaagttgaa aattagctgt gccacctaac aaattcaatg
40021 agttgttgaa acggactgtt aacattagcc tgtttttttg tcttgtgaaa attttattca
40081 tggctgggtg tggtggctca tgcctgtaat cacagcaagt tgggaggcca aggtgggcag
40141 atacttgagg tcaggagtta gttcaagacc agcctggcca acatggtgaa accgcgtctc
40201 tactaaaaac ataaaaatta gccatgcgtg gtggcaggca cctgtaatcc cagctactca
40261 ggaggctgag gcagaagaat tgcttgaacc tgggagacag aggttgcagt gagccaagat
40321 catgccactg cactccagcc tgggtgacag agtgagactc tgtctcaaaa aaacacaaaa
40381 aaacaaaaga aagaaaaaag aaaattttat tcattttatc agctaaaaat acttaacatt
40441 ttttcacttt cctttctctt aaatgtgctt catttgtaat cactattatt atcttacatt
40501 tatcagcagt ttttaaagtg cttccagaaa tattaacttg ttttaaaatt ttaagtgtga
40561 tagaaagcat atattatgaa tctcatctta aagaagtgga aattgactcc agagaagaat
40621 attaaaaatt atttgccaga aatcgctagc Rgctggggtg gggaagggac tgattacaaa
40681 cggacacaag ggaactttac ggggtaataa aaatattcca cattttgaYt gtggtggtga
40741 ttacaaaaca gtacattttt caaaagtcat agaactatat atttcaaaaa ggtgaatttt
40801 cccacatata aattgtaact caatcaattt gactgataca aaatgtMctt accaattgga
40861 tccatggcaa cgatgggttt ggaaggacga cttggtttcc cagaccctct tgcattaatg
40921 gctgtgacac ggtgttcata ttctaagcct tcagtaaggc cagtggagcg gtaccgtgtc
40981 attgtcacag gtactttatt tacacggacc catcgatctg ctctcacttc tttgYgctcc
41041 acaatatatc cagtcacttg ggagccaccg tcatcctctg gtgggtacca agtaagtgtc
41101 atgccatcac gggaaacatc aaatatctgt aatgtttctg ggggtccagg aatacctgca
41161 gcaagacaga ggttaacacg atatggcaat atgaatacgt taaaacaatg actcatatat
41221 ttgtgttttc cttaggtttt ggttggtatt ttaaaaggta cataaggcac atgactattt 41281 ttccaatagt ttcacttttg tactatttgt aaattttaat ttaaatcatt taattattta 41341 attattttta ttaaaaattt ttgactttta gcttcagggg tacatgtgca gtttgttaca 41401 tgggtatatt gcatgatgct gaggtttaat tttgtacttt ccttgctgtt cctcattttc 41461 ttcaattcta catttattaa gcatgcactc tgtatttttt tctcactata acattgccag 41521 tcctccttcc tttcctctct tcctccttcc cttctctctt ttcttccttc cattcctttc 41581 ttcctaactc cctcctcttc tctttcataa gagtgaagat attaaaaaaa aaatcaagcc 41641 taagtgtgtt gaaaacctct gagaaaagga ggtttagaaa cctgagaaaa ggaggtttag 41701 aaaccttaga aagaccacag ggttaatttt gtgacctatY tttatttaca ttagggcata 41761 ttaaaaacaa attctttttt gttaacattc agaatcagag gtggggagag tggtggaagg 41821 gcctgtggac ttacggatgc tgctgcgaca ctctatgacc tcagactgca agtaagagcc 41881 aatcccgaag cggtttgttg cagccacacg gaacacatac tcatttcctt cggtgagtct 41941 ggtaaactta aacgtggacc tagtcactga ttcagaaacg ggcagccatc ctggacgatg 42001 agcgtcacgt tgttctacca cataaccact cagtggagca ccaccgtcca attcaggtgg 42061 ttcccaggaa atggtaattg atgtagcatc aatttcatca atcttaatag gtcctgttgg 42121 tggaccaggc ttgtctatga aagagaagaa atacaggaaa ttaattttta cttcaaatca 42181 aaattgggtg actgaacatc aacttgcttg tatctttgtc acacatcctt accaagaatg 42241 ataactttaa tggtttctga agtagttccg gtaacattct tcaattcaag tgtgtattct 42301 ccagtatctc tgatagtggt ttcacggatg gttaatttag ctactttagt gtgagtttca 42361 actgtgacac gctctgattc tctcagttta gaaccagcaa agaaccagga agcagcagga 42421 ggtggacggc cagcaatagg tatcaccagc tctactggtc tgccagctgg gacatggatg 42481 gtcttctgag gcattgtaga aagatcgatt gttggcaaca ctatRggaaa gaaatcaggc 42541 atttattctt aagcattatt tttatggtaa attgttacga attctgaatg ttttcatttt 42601 actttacttt tttttttttt ttggctattt ggtacatacc tctgaggtct tgtacagtca 42661 cggctgtgac agtctctctt ggttctgaca cacctttctc attttgtgcc cttattctaa 42721 ataagtattg ttcaccttca tttaaggaag taacagtgtg ctctaggact gtgggtttta 42781 aggtgacaac cttcatccat ctctctgtgc cagctttgca ggcctcgaga acatatccag 42841 tgagtcggct accaccatcg tagagtggtt tttcccaggc aagggtaaca gtggatttgg 42901 tgacatcgac cacttctagc tttgcaggca ccaaaggcac ttctgagacc agtactgcat 42961 cagatgtttc acaaggttct ccaatgccat aaatattttc tggcagcact ctgaaRtagt 43021 Rcatggttcc ttctacaagt ccagaaattc tgtaaagtgt cttgctgcat ttggtagtta 43081 ctgttttgaa tgctctcata gcagcttcac gcttctcaac gatgtaattg ttgactggag 43141 ctccaccatc aatcgtggga atttcccaag ttatagtcac agaatctctt gatacttcct 43201 taactttcac tgaaggacag ggaccaggag tatctgaaaa acagaatgaa agattcatat 43261 ttcctatttg cctgatagct ttttttttct tggggaaata taatcaacat agttcctttc 43321 tagagaggtg atcaatcata ggtcaagcta tattgtttta gaggatctac tcattgtata 43381 gccaatttta agtaaaatgc ttaccataga ctttaacaag gactgttgct gatttcttgc 43441 cagattggtt ttcagcttca attgtgtatt ttccagcatc gtaccgatta actttgtcca 43501 ctattagcaa tgagtagctc tcagtggtgt caataattgc ccggcttgca aggtcaatgc 43561 cctgcttgct ccacgttatg acaggaggtg gtcttccaga tacagacacc atcaagcgca 43621 aggaggcccc agccctgatg gtcacagtct tctttagatc atctgcaagc tcaagatctg 43681 gtatttctgg aaagttaatg acaaaattta attaattcat ggacaggtgt gagaagaaaa 43741 taattcacct aaaagcttct gtgataaata cctattcttt ccacgggctc aatttcagca 43801 attgattcgc tgtattcgct catacccttc acgttcacgg cagcaactct gaaggaatat 43861 ttctggccca ttttaaggtc tggcacagtg aattcagtat ttcttattgt ggcattggta 43921 tgcactcggt accactgatc agtgtccttc tcttgcattt ccagaacata tcctacaatg 43981 tcagtaccac catcgtacat gggtttggtc catgccaaac taatgctgtg tttggtggta 44041 tccacaactc ttggagcaga aggtggtcca ggggtatctg tggaatttaa aaagtgagat 44101 gcagatatta ggcaaataag gaaatgttga gatttgtatt ttttgttttc aagactcaca 44161 aaggcattat tgctcttgtc cacactcacc aagataatct ttattaactg ggctcctgat 44221 attataggac agcacagagt gccacattat aatttaatat tattatctta agaattaaat 44281 tgctttagga aagtagatat ttcttcccta cttctttggc cagctttact gtgtgttctc 44341 tattagctaa gctcattact taaagtaccc attcttaggg acttttcttt agttgcttat 44401 ctttcactgg gttagtatca cttgccacca atacatttct aatgaataca agaattggtt 44461 tccttagctt ccccaattca gtcccagtga ctattccttt atagttcttg ggctcagact 44521 ctgtaaaagg atttgccact tatttttgaa gctcttgtca caattacttt gtcagtaact 44581 tatgaaacca ctagaaactg tatgttcctg ctaaacctct tcttaaaagg agttttgcca 44641 tttttctttt cagttattac agtacaatgc atttttctct aaggaaaatt tataaaatag ■ 44701 attcttttca tattgataga atattttttc attaattgga aaatgctgta caacttttag 44761 caattctaat tgctaactta acagatgtaa tagttaaaat gttctctgac ttaagctata 44821 tttatgatca tgcaaataag catgtgcttt tctagaccat tcataaattc taaaaattct 44881 tagagattgt gtgttggaaa attatctgtc atatagtttt gaggagcatt gtatttatgt 44941 atgattcttg gagctcacat gatggttctc tgcatgagat gaagttggaa gSttcgctgg 45001 gctcactgtt accagctgca ttcactgcgg tcacccggaa ctggtaatca caaccctcca 45061 tcaggctggt caccttcagc ctggtatcat acaccacata gtctttgttg acacgtgtcc 45121 agcgcaggct ctYcttctca cgtttgtcta ctaggtagtt gctgatttca ttgccaccat 45181 cagattcagg ttttgtccac tgaatgatga tatgctcttt gccagtccca acttcttcag 45241 gtatgccggg tggtgatgga atagctgttt atgaaaataa ggatgatgag aattgcacaa
45301 aattactatt gataagactg cccttctccc ttctccccct gattctatta catttcaact
45361 gtcaaattat ttaaaagtgt taatacttac tgaatgagtt tctggctaca attggctctg
45421 attcaacagg cacaccaggg ccatatttgt ttactgccct cactcggaat atgtactcat
45481 tgttcttgat gagcctggta acgacatagg atagggttgg gcattcgcct tcaacaatca
45541 cccagttgag cctgctagtc tcgcgtcttt ccacgatgta gtgagtgatt tctgctcctc
45601 cgtcttcctg cggaaggYtc caggctaaag tgcacttctc ctgtgttact ctgctgacgg
45661 tgagctttcc acatgggcca ggggaatctg aaacaggtgt aatgacaagc catgatgaag
45721 atcattcttt ctgcccacac tggaaaatgc tatataaatg tgagaacatt tgacatgctt
45781 aYcaagcact ttgaccatga cagacacggc cttggtcccg ctgKcatttt tcactgttaa
45841 agtgtatttt ccactgtcac ttctgtcacR gaacttgatc acagcagttg ctcgtttgcc
45901 agtatactgc aaagagactt tttcacagag atctagctcc ttgtctcctt tggtccagat
45961 aattttgggt tcaggtttgc caccaactgc agcatccaga acaagatcag aacctgctct
46021 gatggtaacc agatcaccgt gtaatcgggc atccagttcg gctttgggtg gagctgtcag
46081 taggcaaaaM agatatgaat gaatatctga gagtttattt tcacataaat tgagataatt
46141 ttaaaaagga gaatgttgac tatttcctac cgtattcatc tcggcaagtg acagggcctg
46201 tagattcaga ccctttgcta attacgcctg ctgcattctt ggctaacaca cggaactcat
46261 aagtgtctcc ttcactgaga gcagtcacgg tgaagaaatt gtcagataca atggtgtagt
46321 tgcacttcag ccagcgacca tctcctacct cactgactgg cttacgctct atgatgtagc
46381 ccacaacctt gctgcctcca tcatacgctg gggcagacca aatcagtgat actgttgatc
46441 ttgtgacatc tgtcacctct ggtctgcctg gtgcatctgg aagggatgca aaaataaggt
46501 taaaatatRc cactctgagt tctactagaa tcagtaataa taaacaaata tgcccttaaa
46561 ttgtgataca taccaactgg attttgagcc attataggtc ttgaagcttc gctggccttg
46621 ctaacacctg cagcattgag tgcataagtt ctgaactgat attccagtcc ttctactaaa
46681 ccagttactt tgtagttgca ctcϊaagcat ggcactttgt tttctttcac ccaaagaatt
46741 gtattacgtt ctttcttctc aacccagtag ccaatgattt tactgccacc atcgtggtag
46801 ggttcttccc aacgaataga catggcattg gcagacacat ggtagacctc aggtctggta
46861 ggagcgcttg gtgggactaa atataaacaa aggtattaag tatgaataca attttataaa
46921 attcagggga aacatttaat aatagagact ttgaaatagg atttttattt ttcttaccaa
46981 atggatgttc agcaattatt ggtgctgatt ctagaggttt gctgacacca aatctgttct
47041 ctgaactgac acggaaagaa tattccatgt attttgtgag atgagtgact ttaatttgag
47101 tgcgcttgac actggaattg acaagctgcc aagctgttgt acccgattca cgcttttcaa
47161 ctatgtaatt agtaatttca gtgcctcctc catctttagg ttctccccat gacaggacac
47221 acgattcagc tgagacagat gagacctcaa tggggccggt tactggacct ggccttccaa
47281 tgaccacaac tgtgacgcta aatgttttaa caccagctgt attttccagg gtcaagaagt
47341 atcttccaga gtcacctctc atgctgtcct ttacagtcag tgtggttctg tcttttgttg
47401 ttgtaatgct cactcgatct gtctctttaa gtctcatttc ttccagtttc catgttactt
47461 tgggggcagg acgaccactg attggtacgt caatggtaaa tgggcttcct gctttgcaag
47521 ttataagctg attggtaatt ccagtgaggt cagcagtggg ctcgatttga ggctccttaa
47581 tgatgacaga agagaaggct tctctgggtt cactatagcc agcatcattc ttggccttca
47641 cacggaattc atattcagtt ttctcaacaa gacgctctac tgtgaaagtt agctgtttgg
47701 tgtgaccagc ttcaacccag aagtcagatc ccttttgtct catttcaagc aggtagccag
47761 tgatccggct gcctccatcg tggtcaggtt taagccaagc taagactgcg gaggatttgc
47821 tagtatcaac aacatcaagt ctcctaggtg gagcaggctg ttctgtggct acaattggtt
47881 ctggcatttc atagggctca ccaacaccat actcatttac tgcagaaaca cggaaatagt
47941 acggaacacc ttcRgccagg tcattgacct tgaagatctg acgagtgcat ttttcactga
48001 taacctgcca actacggcga cttgcctctc gtttctctac cacataatga tggattcggg
48061 caccaccgtc aagaagaggg gcatcccaca tcaatgtagc agatccccgg gtcacatctt
48121 tgaaggtaat tgggccaggt gggcctggag tgtctagcac tttcacggtg aatgtgattg
48181 acttactacc actgttgttt tccacagtaa gggtatattt ccctgcatca tttctgttgc
48241 agttttccac agtgagggtg ctgaaggaat ctgttgtatg gatatcagcc cgaaggctaa
48301 ggttagagtc tggtttgctc cacacagctg taggagtagg tctaccttgg taggcaatga
48361 agaggcgaat actggcccca gctctaacaa catgagtctg tttgaagttt gcatctatgt
48421 ctaactcagg agctgttaac cggtcaactg ctttaattgt gccagtcact tcacagctgt
48481 cgccttttcc agcaccattg atagcactaa ctcggaattt gtattcttca cctgcttgta
48541 gatcagtgac tgtatatctt gttttcacac atgcctctgc attcaccttg tgccagtctc
48601 ctaagtcggc cttacacatt tcaataatat acccaattat ttccatgcca ccatcaaaca
48661 ctggtttgga ccattctaag ctcacagttg tctttgatgt gtctgttact ttgaccactg
48721 tgggaggacc tggtgggttg acgggctctc tacatttaat gagtcttgag atgccacttg
48781 caggtccaac tcctgcagca ttttcagcca tgacatggaa ttcatactca ttgccttctg
48841 tcagaccagt gactttgaat cttgtttcag agattggtcg tttgctgatc acttttaccc
48901 atcttgtgct tttcttttct cttctttcaa ggatatactg ttgaatttca ctgccaccat
48961 ctgattctgg ccttgcccat gtcagggtaa tgctgttgcc tgttatgttg ctaggttctg
49021 gaatgccagg ggcatcagga acagctgtaa aacaaaaaca aaaccccaaa tcaattagat
49081 gcatttgctt ggaaggttaa acttttgaca tagtaccgct tagtaaaaac gcaaacttac
49141 tatattgtat ttgagcaacc aYtggatcag aatcaagtgg cctgccaaca ccaaacttgt 49201 taactgcaga aacacggaat tggtattcat tgccgtttat tagtttaatg gcagtgtaac
49261 tttgggcttc acatttatcc tcaattagtg cccaggcaag tctgctggtt tcccgttttt
49321 caatgatgta gtgggttatg ggagcacaac cgtcattgag tggggcatcc caccacagag
49381 tcatcttctc cccagtaata ttggtgaatc ttattggccc aactactttt cctggtgtat
49441 ctataagaaa aagtttctag agttagtttc tttttcMttg tccattaaaa tacaactagg
49501 catgtctcta atccaagttc ataaattgta gcattaagaa gctattttaa aagtaccttg
49561 tactttcact ttaatttctg cttttgcaga accagatgca tttttggctt ccactgtgta
49621 tacaccacga tggtcccgag tagcatctct aacaaatagg ctggtggatc caagtacgtc
49681 ggttatttcc atattcatcc tcttttcgat ttccactcca tctttgaacc aagttactcg
49741 aggcactggt cttccttgta ccaaagcttt aattctaagg ctctctccgg acttaataat
49801 gagaccatca aagtattcag ggccaaactc tactattggt ggaactataa aagaaagaga
49861 aatactatgt attagtttgg cttaataaag acatacataa atatatattt ttcagtagtg
49921 ccatatcaca aggccaggaa gagtagtgac ttctactgtg ctaccaaagc ctattttgaa
49981 agtaggattg ataattgaga atttggacta tggggttaaa tatttgccta ttattattcc
50041 ctttgtaatg cctgtcatgt ttttaatttt gcattgctct ttgtaaaaag gaaagtgaaa
50101 catttacaga tcatgtactt cctaatactg ctgcacagtc acacaagttc ttttaagagg
50161 ccatcagctg cattcaagca aggggatgat tgtgttgttg gatgtatgtt gtattttaat
50221 aacttcagta aagatggaca cttattctcg agaacctttt Gtttatgtac tgttaagtgg
50281 cgaaaattac aaagctaaaa actcattccc acttgctgca tgctctttat atgggaatgg
50341 agtgaaataa tagaaaaaat aattgatgcc aattattttt ttcttctttt catctgaaag
50401 gtagaaagac ataattcatc cacttagcaa ccttgggtaa tttgtttctg ttaccatttt
50461 acaggccagg ggccaaatgt gttttttaaa aatgtgaatg ctcttagtct cattggaaat
50521 aagttgtaaa tgcttaccat tttcatctct tgtcataata atgccagaag actgtgaggg
50581 cgggcttata gttccaacag catttcttgc aattattctg aactcatagc gatccccagg
50641 actgagtcct gtaactgtgt actgacattc actgacgtca gtaaagttgc atctcaccca
50701 gcgttcattt ccttgccgtt tctcaatgct atagcccaca atcttactgc ctccatcacg
50761 caatggtggg ttccatttaa gtgtgatggt ttcccgggtg acatcaatgt agtcaggagt
50821 gccaggtggg tctaaaaaat tataaggaag gtaaatgcat cattRcattt gtaaccaggt
50881 cttaatcatc catggaagaa caatacaatt attctataat tttaataaat aagtaaaatt
50941 tttataataa gtaatttatt caatctccca aactcatatt ggtcattaat ttcaactttc
51001 catttcatag tctttaaaat gaacttcaaa ttagtttatt agactgactt aaaataattc
51061 ctcttgcttt acatgaccag ttctctagtg acaagaagat atgtaagaag gtgatgcaga
51121 gaagaaaagc attccttgat atatttgttt ctaatatgtt caattgctaa actaaatgta
51181 tttgaataat aatcacttac ctactggaga aacagctaag gtaaatttgc ttgggtcact
51241 aggtgagctt aggccagcag aattttcagc atatacacgg aattggtaat ccagaccttc
51301 taccagtcca gtggctctat attctcttcc agagattggt gatgtattaa ctttttgcca
51361 gaggatgcta tttctttctt tcttttcaac atggaatcca gtaacttctg aaccaccatc
51421 ataaactgga gcatcccaag ttagtgacat gccatcagaa gtcacattgt atataactgg
51481 ctttcctggg ggaccaggaa tcctgaactg gtgttttgcc acaataatgc ttgagacaag
51541 tggttggctg actccgtatc tgttttctgc tctcactcta aactggtact cagcatcttt
51601 gactagatta ggaactttga aagtcgttct ggcaacactt gaacacacca tcttccagtt
51661 agtttgtgaa gtttcccgct tctcgatgct gtaacaagta atttctcctc ctccattatc
51721 ttcaggtaca tcccatgaca ggatgacact atcagccttg atttcatcaa atcgaatggg
51781 tcctttgggc tttgatggtg ggccaagggt gatgactgta atggggactg caattctaca
51841 cactgcatta tcaagaataa cagtgtattt tcctccatgc tccttcttgg 'cattcttaat
51901 actcaaagta attttgtttt cagttttact gaagcgaaca aattcacttt ctttcagtgg
51961 caagccatct ttcagccaac tgatggatgg tttgggtttt cccttataag gtaattcaag
52021 gtgcacattg tgcccaattc tcacagtgac ttgtgcccca gggatatctg acaggtcaac
52081 ttcaggtgta ataataagtt ctttcactgt aattggccca atagtgacag gatcactcag
52141 tcctttctca tttttggcaa acactctgaa ctccaggact gactgctcct taagttggct
52201 aacctcaatt tcacatgtct tacttatgcc agcgtgggac cacgtctgtt cacctttacc
52261 tttcctttct acaacatatt ctgtgatgac gctaccacca tcaaagtcag gtttggtcca
52321 gctgaggatg acagatgtct ttgttgagtc tttcattgag agatcacgta taggagctgg
52381 tacttcagca gccttcactg gctctgtagt ttcacagggt tccccaattc caatttcatt
52441 ttctgcaaga actctgaaga agaatggagt cttctccgac aaatctatta gcttgaagga
52501 tgtgctagaa catttgtgtg acacgacaga ccatgttctc ttggtggcat ctctcttttc
52561 aatgacataa ttaattattg gagatcctcc atcaatgaga ggaggatccc agagcaaagt
52621 gactgtgcct cgagaaacat gtttaacctg tagtttctgg caggcagctg gtgtgtcaag
52681 cactttaaca ctcacagttg aagacttagg ttcaccaact ccattttcta ttgtgagaat
52741 atattttcct gtatcattgc gagtaacttg aggaatggtg agtaatgagg atgaatcagt
52801 gttttcaatg ctgtatctgg catcactgcc aagattcttc tcatcttttc tccatgtgac
52861 agtaggtgga ggtctgcctt taaagggaat caacacttgt acatcttcac cagctttggc
52921 aataacagaa gttctgagag ccacatccag gtcaatctct ggtgcctctg tagacataaa
52981 atggatacat acagtgaatt ttaaaagcaa aaaagagtga tttcctggaa gtatgcttat
53041 taaaaaacat aatcaaacca gtaggtacat accaagtata tctttagctt gtacaggttc
53101 attcatttct ataggttctc cttgtccagc acagtttaca gcagataccc ggaagtagta 53161 attgactcca ggtttcaggt tggataccac atattctgta gttctgacct ctcctttggt
53221 agagacagta gtccattcct cttcctctcc ttgtcttatc tcgacaacat acccagtaac
53281 agcactgccc ccatcataga caggcttact ccagccaagg gtgatggatg acttggttga
53341 atctgcgatt cttatcttag caggtggacc tggaggatct ggaatggcca ttcacaataa
53401 aataattaca caatcatgta caatttagtc acacaggtga atatagaaga cacaggtgaa
53461 gatgctgcag gtatgagcac ttaccaatag gatcagcagc tttgtagaat tcagatggtt
53521 cagaaaatgg accctgtcca gcagcgttta cagcacaaac tctgtattga tagtcactgt
53581 tttctgtgag tcctgttact ttttgtctgg tgtcacggat agtctctttt agtactttaa
53641 accatcctag gctcttcttg tctcgttttt caaggaaata gccacttata tcactaccgc
53701 catctgcaat tggcctgctc catacaacag tcatcgaatt cttggtaatc tttgtcactt
53761 ctggtatgcc tggtggccca ggtgtaacta tttagaaagg aagggaaaac aaggtacaag
53821 aatccacatt actgataaat tatacctttg atgttcgcat tctttccaag aacattggtt
53881 ttatttttta tatttttcac cttgtgaaaa ggatggtaat gagacaggtg ttatataaaa
53941 gaaaattaaa gcatatgcac aggttagcgt agtttatttt taaatttttc acgtttcagt
54001 tttctaatga aatcatgtct caccaaatgc gttcttggct acaacggaat cagattccag
54061 tggctcgcca attccatatt tgttcacggc tcggacccgg aagatgtatt catttccttt
54121 gataattttg gtggttgtaa tgatgcactc ttccaaatgt tcagacacca tagaccacac
54181 aacgcggctt gtctcacgct tttccacaat gtagtgagtg atttttgcac caccatcatc
54241 agctggaggc cgccagagaa gggtgatctt ctcagcagtt acagtcttaa attcaattgg
54301 tccaccaggt gggcctggtt tgtctatcag tgaaaggaca aaacacgatg ttagtacttc
54361 tttaaagcct acttgttcaa aattactaag ctttaggcaa atgtaatatg acagtggtct
54421 gtattcagca cctaccaagg atctgtactc tgatggtggc tgaggctgag cccatggcat
54481 tccttagttt taattcatag catccactat taaggcgatc ggcatctttg atgagtatag
54541 atgcgaggtc cgtggtattt tcaacacaca ccagtgcatt ggtttgtaat tctttatcat
54601 ctttatacca ctcaatagta ggcgcaggtt" tgccagaaat gccagctctg agcttcacag
54661 atgtacctgc tctgtatttg acaacttctg tatattctag aggcaaatca attacaggac
54721 cacctgcaag aaaaacagat gagaaatatt taaaatatta taaggatgga aaaaaaaata
54781 gttgcaccaa ccttaaagta aggtcattgg ccattattaa attatacaca cacaccatca
54841 ttataattct atgcaattat tatttaaaaa ttaaaaattt ataggccact cttctatctc
54901 agatggggta gaaagactgt gagttgtgca gggacttaag caccttttac atgtgtttta
54961 aagtccatta gttgctttat tccagtgaag aaatgggata tggtagtgag aacatcatgg
55021 aagaagctgt taaaacattt cctttcactt tagcttttct ttccttttct tctacgcacc
55081 tcctattctt tttcttttcc catcccagtt cactgatatc caagtgtgga tggttcatgt
55141 cagacactta aagtctgaat tctctaccca cagagctaca taattactgg acagattttc
55201 tgccatgctg tttggaatta tcaagaaaga taactaaatt cgacttgctt tatactctca
55261 taaacactga ttcagtatcc acaaagcctg attcataaat aatgaacagc cctgagattc
55321 tcagcatgaa gatggaagtg gatacttttg cccagacact acagtggctc aattttctct
55381 tagcaaatgg agatctttta ggtaaagtgt cacaggatac ctgactctca tgacgtttta
55441 attttcaaaa cattgttgag ttgaatgtgc cacttatttt ccagaatggt gaagaatacg
55501 cattttggac taaccatgat agagagggct ctgaagtctg tctgtctgaa ttcccactcc
55561 atccctaacg gggaagttac aaaagcactc tcagcctcag tttcccttct ataagatggc
55621 taatatgagg gctgtgagaa ctaagggaaa gaattcatga aaattcctca ataaatgtta
55681 gctgctgcta atactatcat catcaaagtc tgtgtttaga ccaggcatgg tggctcacac
55741 ctgtaatccc aacactttgg gaggccgagg tgggtggatc acctgaggtc aggaggttga
55801 gacctgcctg gccaacatgg tgaaactccg tccctactaa aaatacaaaa aattagctgg
55861 gtgtggtggg gcatgcctgt aatcccagct actcaggtga ctgaggcacg agaatcgttt
55921 gagcccagga ggcggaagtt gcagtgaacc aagaccgtgc cactgcacta cagcctgggc
55981 gacagagtga gactctgtct caaaacaaac aaacaaacaa acaaacaaaM aaaaaaaaac
56041 caaaaaatga agtctctgtt tagtctgtgg cttatttaag agcaatattc tctaggaact
56101 caaccatggt ttaattattg actctgataa tttaaaacag aatgaaagca ccaactcata
56161 caatcaatca taaaaggttg aagttggaaa ggcccataga tatcctgagg tctaaatctc
56221 tcactttaca gtcaagtcaa gagggctgga aaggtattta aggaattttc ctcagactcc
56281 cagctagtcc ataatttctt ccaaaaaccc cattaaggca cagagtaaaa gttattctat
56341 aggattagaa ccaggaaaag gtatgcggaa atactgagtt aaatagcaat tttgattcaa
56401 agtgctaagc atgcttacca taagaatcga tgcaagtaat gggccctaca acctcagatg
56461 gattgctaat ggaaccaaca gcattcctag caaagacacg gaattcatac tgggaattct
56521 gagtcaagcc agagatgatg aattgagttt cagtaacatt ggtgaagctg gccttggtcc
56581 atctgccatc tggaaggtca cgtctctcaa caatgtagcc tgtaatcttg ctacctccat
56641 cgaaagctgg ttgttgccat gaaaggctga cagagttctt tgaaatatca gtgatacgaa
56701 catttcttgg gggttctgtg gtaataagag aagcagatta gcggcactta taatattttg
56761 cttcgcagaa gtaagatttg catttttgtt atcagaactg cccttcccat gacaaatacg
56821 taccacaagc atcaattgcc aagactggtt cagaaggatg gctaggtttt ccaactccag
56881 cagcattttc tgcatacacc ctgaattcat aaataagtcc agcactgatt gttgtgactt
56941 tgaagtgagt tgtgcggatg accagtttgt tggctttttg ccataaaata ctgtttctgt
57001 ctttcatttc caggtgatag cctacgactg cactgcctcc atttgacact ggttcatgcc
57061 agcccacagt gatgctttct cgagtaacat tagtgaccca tggtgtagat ggtggtccag 57121 gtgttgctac aaaagagaga aatcctatag attagtacag acaataacac atttatggta
57181 aaagaaaacc tggatctttg aaaagttgga caacttactg tatggtagtt tgacagtcac
57241 acaagctgaa tctatatgat cactgatgcc aaagcggttt tctgccttga tgcggaattg
57301 gtattcttct cctgtggtca gtttcatgac tttcatcatg gttcttgcaa ctgttgcaga
57361 cacttcagtc cacactgcag tacttgtttc tcttttgagt agaatgtagt tggtaacttg
57421 acttccaccg tcatacttag gtggctccca tttgagagtc acactttctt cagttatatc
57481 actaataaca acagggccag ttggaggacc aggcctgtct agcacaacaa tgttaatgaa
57541 agctttggtt gtaccactgg agttggcagc agtgatttca tatctcccag cgtcagctgt
57601 aacactttct ttgagattga tggtcaggtt ctcagcagta atttcggtat taaatctcat
57661 ggttgcctta agggggacac catctcttga taaggtcact ttgggaagtg gttttccaga
57721 gattggaatg tcaactttaa ggtttgaacc agctctaaca tatacagtgt gacttgggaa
57781 attcttcata tcaatttcag gtggttctga aaaatgagta tagaaagtga aagtgaaaaa
57841 gtgtttctga aaattaacat aaatcaatgc aaataatttc aaattttgct tctacacaaa
57901 attagacata cctagttgct ccttaataag aacaggaagc agaagctctc tcgggtcact
57961 caggccagct tgattttcag caaacactcg gaaaaagtat tcagaattct ctctcagacc
58021 ggaaacaacg tgatgggttg actttgccac tgcacattta acccagtttt tctgtccttt
58081 ttctagtgct tcaacgacat agtgtacaat tctgcttccg ccatcatgtt caggcttcag
58141 ccaggtcagg gaaacactgt ctttggatat acttgttact ccaagttttt caggtgggct
58201 tggtttttct aagaaaacaa agcatcaaaa gaaagtgaat aattattaca gcacttttaa
58261 atgtgcactc ttcatgtatt gtcaaaagaa aacttttaaa tgaaggccat atttttatgt
58321 tgattttagt cttccttttt acataaccca ttttgcttct gaaccaacca aggctcccac
58381 aagctatgtc attacatgct atagcattca aatccaaatt aagggtaaaa tgaccagatt
58441 aaatgataaa atttactcca aattcaccat ggggataaaa aagaggaaca agatgaacaa
58501 tcttttaaaa aactttatat acaatttctg tacacacgca catacacaca tatgatatat
58561 gtcatacaca catactatat atatata'atc tgtgtatata tatatataaa catgtatacc
58621 tacatatata tacagttaga ttcaataata cttaaattca acatatatta tccaatttta
58681 gctacgtaaa tttctgtttt tttaatcaat gaaagactct tcactagaga ttccattttg
58741 tgccatagta tgaatagttt ggggtgtgaa gggtgtgaac ccaaaagcat ttaagatgaa
58801 ataacgacta attagaatga ctcacactat tctagcaaaa ttaacgtgga tatgtagaat
58861 ttccttattc ttaaaacata cctgttattt ttactccttc ctttgtttca gctggtatac
58921 caacaccaaa ctcattttct ccagagactc tgaagtaata gatagctcct tcttgtaaat
58981 tggtaacttt gtaggagagg cggttacagt tgttggtcac agagacccat gctttcttgc
59041 tggcctcacg tttttctatg tggtaattct tcactggtgc tccaccatcg ttttcaggaa
59101 catcccagga taacactgca gactctttgg ttacatcttg cacagtaatg ttggttggag
59161 gacctggggt atctaaaact ttgacaacta aggtcagtga agcagcactc aaaacattct
59221 gaattgttaa tgtgtacttt ccagagtcat ttctgttggc attttcaatg gtcagtgatg
59281 tacgagagtc tgtggtatca acataagctc tagtacggag gtcagtgtct ggcttactcc
59341 acaagacatt gggtactggt cttcctcgga aaggcacagt catggtaaat gaggcaccag
59401 ccttgacaat caaggtcttc ctcatttcac tgtcaatatc aaataaaggt tcttcttctc
59461 tttcctttgc tatctgaggg tcagagctat cactggggtc actagcacca accttattga
59521 cagattttac tctgaaaaca tattcagctc ctgttgttag tccgcttatt gtatattctg
59581 tccctcgtaa gctctgaatg gaagttttcc agtcagtgtc atcagatttt ttgtattcta
59641 cagtatatcc agttatcggg gccccaccat caaacagcgg cttaacccag gcaatagtta
59701 tagttgtctt gcctgagtcc acaattttgg gtttggatgg aggagatggt aggaacactg
59761 gatcttctgc cctaattaag ggagaggttt cacttggaag acttaggcca gcagcatttt
59821 ctgcataaac acgatattca tattcacatc cttcccgaag tcctgttgat ttcactctta
59881 gatcataaac tggttttttg tttacacgca cccatcttag gctatttttc tctcgccttt
59941 caattatata tccagatatt tcactacctc catcactctc gggtcttgac cagcaaagtg
60001 tcatagattc tttggtcaca gaagtaattt ccaaagacgt gggtggactt ggaactgtaa
60061 atggatctag tgcctttata gctacactct ctaggggctc accaacacca tatttattaa
60121 caccagttac tctaaatata tattcattgc ctttgagtaa cttggttact ttacaggatg
60181 tcatctgtaa ctctccttca catattgtcc atgcaaggtg gcttgtttca cgtttttcta
60241 cgatgtaata gtcgatatct gcaccaccat cttcttgggg acgtccccag gaaagagagc
60301 atttctcagc agtgaggcca tttatttcaa gtggtcctgc tggtggacca ggcttatcaa
60361 gtactttgca attaacggcc acagaccgag tgccggcaac attcttcagt gttagtacat
60421 attgcccagt gtctcgtctt atacagtctt taactgttaa caaagtatga ttgtctgttg
60481 agatgatttc tgttcttgct ctttcttcaa tttctatacc atccttggcc caggaaatta
60541 ctggcagagg tcgccctgca atgtctgcat ttatcttaag gacctctcca gctttgacaa
60601 caataacgtc tcggaacttg acatccatca taactcttgg aggctcaaca tcatctttaa
60661 ctataatagg cccagtggat tcagatggct cactaactga gtcagcagca ttccttgcaa
60721 aaacccggaa ttcataacgc tgatcttcag taagttcagt tacttcaaag tatgtttctt
60781 gtatattagt ataattgcac ttcagccacc ggccatctgg tagttctctg cgttcaacaa
60841 tgtatcctgt gatcttagct ccaccatcat aatgtggttt agaccattta agtgacactg
60901 attttcttgt gatatttgtg acttcaggtt gtccaggagg gtcacaagga tctcttgcag
60961 ggactggttc acaagattta ctgcatttac caattccagc aatattttca gcatatacac
61021 gatactcata catcagtcct tcatcaaggc cggagacttt catttgagta tcagcaatga 61081 ggattttatt tgcttttgac caaagaatgc tgcttctttc tttatactca agatgatagc
61141 caattactcg acttcctcca tcattaactg gcacttgcca ggttacaagc atggtagatt
61201 ttgtggcatg cacaacttta ggagtaccag gaggacctgg gggactgaat ggatactctg
61261 caacaacagc tgaagattca ctgtaggagc tctttccata gcggttttct gcacaaacac
61321 ggaactgata ctcacttcct gttgtcaggc gaactatttt aatggatgtt cttgcaactg
61381 cttgtgaaac tatgtgccat gttgtagagg tggtttcttt cttttcaaca atgtaattgc
61441 taatttggca gccaccatca tattctggag gattccaaga aatggttatg ctgtcacaac
61501 taacctcatc aatacgtata ggacctggag gtcctggtct gtcaaggaca attatagtaa
61561 taggaactgt tatggatcca gcactgtttg aaacacataa ttcataagtt ccaacatctt
61621 cctttgaagc ttccttaata gatagtgatg ttacagtctt tgaagaagaa acattgactc
61681 tagttgtctc tttaagagtc tgaccatctt ttctccagtt cacagtagct tgaggtcttc
61741 ctttgaatgg cacatcaatc ttaagttgtt ctctagcctt tacattgaaa gtatggaaag
61801 gaagctcaac tgaaggcttt atttcaatat cccttgcaat tactggcact ccaagttgtc
61861 ttggatcact tcttcccttt tcgttaactg cagctaccct gaagacatac tcttctcctg
61921 cagttaagcc agatatagtt gcttctagag tcttaacttg tgtgcaggtg ctccactttt
61981 cactcccttt agtctgcatt tcaaccacat aaccagtaat tttgctgccR ccatcacttt
62041 ctggtttctc ccacttaatt gtagctgtat tacgggtcac atcaacaaga gttactcttc
62101 oaggtgggag gggtggttca gacactttaa cgggttctgt tgtttcagct ggcaaaccaa
62161 tcccatattc attggaagcc aagactcgga agtagtaaga acatccttct tgtagatttt
62221 caattctgaa agtagtttta gtgcaattat ttgtaatggt agcataggct tttcttgtag
62281 tttctcgttt ttcgacaatg tagtttgtaa tcttagctcc accatcaata agtggtggtt
62341 cccaggacaa cgtcactgag tctttcttca cttctcttaY ggtcaaattc acaggggcac
62401 ttggtgagtc aagaactctg acgttaacaa aagctgtttt ggagccacta ttattttcta
62461 atgtcagatt ataccgacca ctgtcaaatc tggtaacatt atcaatcacc aacattgtaa
62521 atgagctggt cacctctatc tgagccctgt cagtgagaat gccttctgcc ttttcccatt
62581 taacttcggg ttctggtcga cctttgatag tgacaaataa gcgtaaagta gcacttgcac
62641 gcagaacgac cacctttctg agatcagcat cgagttctat ttctggtggc tctatcctct
62701 cctgagcaac cactgagcca ggtagagttg caggttcacc tacaccttca gaattgatgg
62761 cacaaatacg gaagttatat tcagtgtttt ctttaagctt ggtcactgtg aactgctttc
62821 cttgtaatcc tgttggtgga gtgcaggttg tccattcatc cgcagcagct tctttgacct
62881 ctacaacata gcctttaaca ggtgcgccac catcataaat tggcttactc catgccaggg
62941 agacagaaga tctggaagtg tccgtcactt ttggattgct tgggggacct ggtggataca
63001 aggcatcaca cgcacggtag aaaacagatg gctcactagg ttctcccaca ccagctgcat
63061 tttcagcagc aactctgaat tcataggaat ggccttcggt aagaccagtt accctgagcc
63121 gcagatccgt taatgttttc ttgttgcact tggtccatct aacgccttcc ttatctcgtt
63181 tttcaagaat gtagccctca atttcggtac ctccgtcgtc tactgggcgt gcccatgtta
63241 ctaccataga atctttggtg attgctgaga cttcaggtgt tgagggagga cctggtggct
63301 tataaggatt acaggccgta acaggcccag attccaaggg ctctccaatt ccatatttat
63361 tcacagccat gacacggaaa atgtactcat taccaggaag aagtttagta actttgtagt
63421 taagggcctg tacctcagtt gaaacctggg tccaagagag tcggcttgtc tccctctttt
63481 caatgatgta atgtgaaata ttagcaccac catcttgcaa aggtgggttc catgccaggt
63541 aacatttttc cgcagtaact ccagtaactt tcagaggccc ttcaggaggc cctggcctgt
63601 caagtacctt tacagtgatg ggtatagact ttgtaccacc aacattgctg agtttcagaa
63661 tatattgtcc tccatcagtc cgtatacagt ctttgacaac aagagttgtt ttctgaatag
63721 tagatttaat ttccattcta gcagctgttt cttcaagttc ttttccatct tttgaccaaa
63781 caacatcagg tataggtttg ccacggatgt cggcttcaag aacaaaagtc tctcctgcat
63841 gaacaacgat gacatcttta tattttggat ccagagaggc atttggtgca tcaatttcat
63901 ctcttgcagt aatggcacca ctactatcag atggttcact aaagtttcca gctgcatttc
63961 ttgcaattac tctaaattca tatctttggt cttctacaag tccactcact gtaaattcag
64021 tttctaatac gttggtaaag ctggctttca tccagcggcc atcaggtaga tctttctttt
64081 ctacaatata acctgttatt ttgctaccac catcataggc aggtttcttc catttcagtg
64141 tgacattgtt tcttgtaata acaatggctt cagggcgacc aggtgggtca catggatcac
64201 gagcaacaaa gcattctgac actttgctag gcttgccaat gccaacaata ttttcagcag
64261 aaactttgaa ctcatactca aggccctcat caagcccagt tgttttgaat ttggtgtcct
64321 gaatgggggt cttatttaac ttgacccata aaatactgtt cttttctttc tgttcaagat
64381 ggtagccaat aattttggtg cctccatcat tcactggctc atgccattgc acaagcatct
64441 gatcttttga gattgatgtc acaaaaggag ttccaggtgg tccaggttct ttaaatggat
64501 attgtacaat aactgcctta gaatccagtg gggcactttt tccatacctg ttttcagcaa
64561 aaattctaaa ctggtactcc gtgcctgttt tcagtttggt tattttaatt gttgttcttg
64621 caactgttgc tgataccatg tgccaagtgg tggtggttgt atctcgcttc tctacaatgt
64681 agttgcttat ttggcagcca ccagtatagg ctggaggttc ccaagatatg actacaaagt
64741 ctgcactaac ttcatcaaac cgaactgggc caactggagg tccaggcttt tctaaaacga
64801 taacactgag attttctgtt gctgtgcctg cactatttgt tgccgttacg gtgtattttc
64861 caaagtcatc tttgttacct tctttaatgt gcaaaacagt tgaKgtagct gtttcttcaa
64921 cgtttactct tgttgtctgt ttaagaggct caccatcttt gacccaagaa atgttaggtc
64981 ttggtcggcc tataactgga atttctattt taagatcttc tccagcttgg atactatatg 65041 tgttaaatgg taactttaaa ctaggctgta tagtcaagtc cttggctatg acaggaacac
65101 ccaacactct tggatcgctt tttcctttct cattataagc cttgacacgg aactgatatt
65161 cttgtccaga actcaaacca gtaacaactg cattacagac tttggattca gccacaatgc
65221 tccatttttc agttcctttg ggctgcattt caacaacgta ccccaggact ctgctaccgc
65281 catcatgttc aggtttctcc cacataagtg atgcactggt ctgggacaca tcagtgagtg
65341 taacctttcc tggtggggaa ggaggttcag cagctttcac ggcatcaaca gtttccactg
65401 gaacaccaac tccaaattca ttttcagcca tgactctgaa gtaataaatg gctccttctg
65461 taaggttttc cactttaaaa cttgttttgc tgcatttact actcacatt'a gcatacgctt
65521 ttctggttga ctcacgtttg tcaatcacat agttcttgac ctttgcccct ccatcaatga
65581 tgggtggctc ccataccagg aaggcagaat cttttctcac ttctttgact gccaaattct
65641 gtggtggtcc tggagtgtca agaactttca cagttacaaa agcagacttt gatccactgc
65701 tgttttccaa cttaagaatg tattttccag catcatttct atcacagtta tctattgata
65761 gttgggtata gtttactccc ttttcaattt ggaccttatc tgtgaattca ccttcctctc
65821 gagaccaagt gatctcaggc Rttggacgac ctttgaatgg aatgtgaatt ctggcagatc
65881 caccagctct tacaacaatt ccttttctta attcggagtc aaggtcaagt tcaggtgctt
65941 caagtttatc ttctggtttc acagtaccag gaactgatgt agcctcacct aaaccaactt
66001 tgttgagggc acagactcgt attttatact cttggtgttc agtgagtttt gaaatttcaa
66061 atcgagtgac tctcaggcca gtctgtggag taactatttg ccattcttct tcatctgctt
66121 tacagatttc tactacatat cccaagatct cactgccgcc atcatagatg ggtttacccc
66181 aggcaagtgt gattgaattt ttagtggtgt ctacaatgtg tgcattggta ggtgggccag
66241 gtttgaacac aggatcacag gctttataat aaactgtagc tggacttggt tccccaactc
66301 cagcagcatt ttctgcagag accctgaatt catactcatg atcttctgtt aatcctgtca
66361 ctcttagacg caaatctgta atgcggcgtt tattacattt tatccatcga atgccacttc
66421 tgtctctttt ctctacaRtg taaccaataa tctcacttcc accatcacta tctggacggt
66481 tccaacagac ggtcatggag tctttggcaa tatt'tgtgac ttccaagctt tttggtggtc
66541 caggaagcac aaatggattt ttcattagta ctggtgcaga ttccaaaggc tctccaacac
66601 catatttgtt gacagccatt atacggaaaa catattcatt Wccttctaag agtttggtaa
66661 ctttcagaga attggtcaca acttctgaag caacaacagt ccaggcaagt cgactggttt
66721 ctcgcttttc aacaacatag tgagaaatgt cactgccacc atcttgaagt ggtggagacc
66781 atgttaaaga gcatttttca gaagtgactc cagtaacctg gactggccct tctggaggtc
66841 ctggtctatc taatactttt acatttactg ggaatgactt agaacctgca acattggaag
66901 ctcttaaaat atactgccca ccatcaattc taattgcatc ttttacaata agtaaagcct
66961 tgaaatctgt gttctttatt tcacatctag cagattcttc aatttcctta tctcctctta
67021 accactcaat ggtaggtagg ggctttccat ggacatcagc ctcaagtctg aatgtttctc
67081 cagcatttac cacaattgtg tctctgaatt ttggatccat tgaaattctt gggagttcaa
67141 cctcatcctt ggcagttatt ggtccagtac tgtcagaggg tttacttatt gcaccagctg
67201 cattctttgc aatgactctg aattcatatc tttgatcttc agtaagacct gacacagtaa
67261 attgagtttc aatgacattt gtaaagctag ctttcatcca acgaccatca ggcaaatcac
67321 gtttctctac aatgtagcct gtaatcatac ttccaccatc atacacaggt ttggtccact
67381 gtaaagtgat ttcatttctt ttaaccatta ttggttctgg ggttcctggt gggtcacagg
67441 gatctctggc tacatagcat tcagaattct tgcttgcttt gcctacacca acaatatttt
67501 cagcatacac tctgaattca tattcaatgc cttcttcaag attctgtgct ttgaattggg
67561 tgtcatgaat aatagttttg ttgacctttg tccacaaaat actgtttctt tcttttctct
67621 ccaggtggta acctatgacg gggcttccac cattgttgac tggttcatgc cactgtatga
67681 ccatggagtc tttggaaatg gctgtggcaa atggtgtacc tggagggcct ggttctttgt
67741 agggatattg agctacaatt ggatcagact ctaaggcaaa gctttgtcca tatctgtttt
67801 cggcaaatat tctaaattgg tattctgtac cagttttcag tttggtcact ttgagtgtag
67861 ttctagcaac agtagcagaa acaacatccc atactgtggt ggttgtatct cttttctgaa
67921 caatgtagtt ggtgatttgg cagccccctg tatataatgg agggttccaa gataatgtaa
67981 tactttcagc actgacgtca tcaaatttaa caggtccttt tggaggatca ggtttatcta
68041 gagttacaat ttcgatggat gctgtcttct gaccaacaac attggcaact gtgattccat
68101 attgtccacc atcatcctta tgagtttctt taatactgag tgtggtgaga tccagtgaat
68161 cggtaacatt gattcttgtg gtctgcttca gtgggagacc atctttagtc caggtaatgg
68221 ttggcttagg acgtccagaa attggcactt ctattttcaa atcttggcca acctgtacac
68281 tgtaactact gaatgcaggt tttacatctg gctcaattac cagatctttg gcaactattg
68341 gaactgcaag ggaccgagga tcacttctcc ccttttcatt tacagcaaca actctaaaaa
68401 gatattcttc cccttgagtt aggYtggtaa ttactgcctg aagagacttt actcgagcac
68461 actctgacca tttctcactg tgtttagctt gcatttccac aatatactga atgattttac
68521 tgccaccatc atgttcaggt tttgtccaac tcagagagac actgtttctg gtgacatcat
68581 ccacagttat ttttccagga ggttgtggca cttctgcaac cttaattgga tcagcagtct
68641 gggcaggaag gccaatacca tactcattct cagctgtgac tctaaagtaa taactgcaac
68701 cttcttggag ctgatcgatt ttccaagaat tcttatggca gttagttaca acagcagcat
68761 atgattttct tgtggcttca cgtttctcaa caatgtaatt ttttattttg gatcccccat
68821 ccaacaaagg aggttcccat gtaattgata ctgagtcttt ggtgatttct gtgactttca
68881 ggttaacagg tggacttggc gtgtccagaa ctctcacagt aacaaaggca gactttgttc
68941 cactgctgtt ttctaatgta agcgtatatt ttccactatc atRtcggttg acattgtcaa
580 69001 gaaMaagaga ggtgaaactg ctagtgacat caattatagc tgcatctcgg atttcaccat
69061 ccacctttcc ccatttaact tctggtgtag gacgaccttt tataggaaca aataacctta
69121 aggagccacc tgcccttata tttatgattt tccttagttc taggtcaaga tcaatgtctg
69181 gtgcttctaa tttttcttca actataatag gtccagggac gtcagcatgt tctccaactc
69241 cagctttatt aatagcacag atacggaagt tgtattcatg cttttccaac agcttctcta
69301 cttctatgtt tgttttatta attcctgttg gtggagtgca cattgtccat tcaccaacaY
69361 tcacatcaca tttttcaaca atgtatcctt gaatttcaca gccaccatca tatattggtt
69421 tgctccaaga aaggaatact gaagatctgg ttatgtctat gactttgggg ttgtttgggg
69481 gtcctggttt ataaatagga tcacaagcct tttggtaagc agaaggaggg cttggttcac
69541 taagtccagc agcattctca gcagaaactc tgaaotcata atcgtgattt tctatgagtc
69601 cagttactct caggcgcaac tctccaatca gacgcttatg gcatcttgtc catctaatgc
69661 cttctttatc ccgtttctca agaacatatc caagaatttc actaccacca tcagatgctg
69721 gtctttccca tacaacaatc attgagtcct tggtcactgt tgtgacttct ggagcttttg
69781 gtgcatctgg tactacaaat ggattcttgg caactactgg ctcagattca agaggttcac
69841 caacaccata tttgtttact gccattatac ggaaagtata ttcattgcct tcaagaagct
69901 tagtaacctt gcagctgaga gtctgcacat tggcatcaac cacagtccaa actaagcggc
69961 tggtttctct cctttccaca atataattta tgatgtcact cccaccatcc tgaagtgggg
70021 gtttccaagc tagtgtgcat ttttctgctg taactcctga gataacaaca ggtccttcag
70081 gtggccctgg tctgtcaaga accttgacat tcacagtaac tgatctttct cctgcaacat
70141 ttttggcctt cagtatgtaa tttccactgt cgacacgtac tgcatctttt acactgagac
70201 tggtggcaaa gtcggtgctc tttatttcta atcgagctgt gtttgaaagc tcctgatcac
70261 cttttatcca ctgaatggtt ggtattggtt tgccataaat atctgcatca accttgaatg
70321 attcaccagc atgaaccacg attgtgtctt tgtattttgg atccatactt attcgtggtg
70381 gatctacctc atctctagct gttattgctc ctgtgctttc tgaaggctca ctaaacactc
70441 ctgcggcatt tcgggctata acccggaact catatctgtg atcttcaact aggccagtta
70501 cttcaaaatg agtgtcaata atatttgtaa aactggcttt catccaacgg ccctcaggta
70561 attctttctt ctcaacaata taaccagtga tcttgcttcc accgtcatag gtgggtttct
70621 tccactgaRg agtcacagaa ttccttgtga caatgattgc ctctgRccgt cctggtggat
70681 cacatgggtc acgagccaca taacattctg atactttact cggcttgcca atgcccacga
70741 tgttctctgc agagactcta aattcatatt caacaccttc ttcaaggcca gttgtcttaa
70801 acttggtttg aggaataggt gttttattca acttaaccca gaggatgcta tttctttcct
70861 tgcRttctag atgatagcca atgactctac ttcctccatc actgattggc tcattccatt
70921 gtacttccat gctgtccctg gaggacagtg tgacaactgg agtgccagga ggaccaggaa
70981 ctttgaatgg atattgggct acagtaggct ctgaattgag gtaggtactc ttcccatatc
71041 tgttttcagc tgcaattcta aactgatatt cacatccagt cttcagtctg caagccttta
71101 ttgttgtcct tgcaactgta gctgatacaa tttgccaggt ggttgtggaa gtgtcccgtt
71161 tctcaacaat gtaattattg atagaacttc caccatcata cttgggtggg ccccaggaaa
71221 gagtaatact atcagctgtc acttcatcca ttttaactgg tccagttgga ggccctggtt
71281 tgtcaagaac gataacatta agggtttcaa tagcttcacc agctgagtta gtcagtttaa
71341 ccacataatg gccaacatct tctcggcagg cgtcctttat tgtcagtagt gaattatttt
71401 ctgtgctctc tgcatttact ctagttgtct gcttcagtgg tacattatct ttatgccagg
71461 ttacagctgg ggtagggcgg ccaatgaatg gaacatcaac ttttaggtct tcacctgcca
71521 gtacagtgaa agtattgaac aggagtttga aggctggtgg aatgacaaga tctttggcga
71581 tcactggcac actcagttgt ctaggatcac tgatgccctt ttcattctga gctgaaacac
71641 ggaaagagta ttcttcaccc tgaattaatc cagtgatagt ggcttcagtg accttgactg
71701 tggcacacgt ggcccatttg tcactgcctt tggtctgcat ctccacaatg tagcctagaa
71761 ttcggctgcc tccatcatgc tctggtttct cccaagagag tgacacacta tttcttgtga
71821 catccatcaa agttattttt cctggaggaa gaggtcgttc tgatgctttc acagattctg
71881 cggtttcagc aggcagccca atgccatatt cattttctgc gagaaccctg aaatagtagc
71941 tacagccttc ttgaagctgg tctaccttcc aggaagtctt gtggcagttt gttgcaacag
72001 ttgaatatgc ttttcttgtt gattcccgct tttcaacaat atagttcttg atttttgaac
72061 ctccatcaag gagaggtggg tcccatgtga gtgtgacaga tgtcttagtg acctctttta
72121 ccttcagatc ctgtgggggg cctggtgtat cgagaactct aacattgaca aatgcagact
72181 tgctgcctga actattttct acagttagta tatatttgcc actgtcaaat ctgtttacat
72241 ttccaacaat aagcagggtg taagagcttg tggattcgat gctagcttta tctaaagatt
72301 ctccatggtc ccgggcccac ttcacctcag gtgcaggcct tcctttgatg ggaacaaaaa
72361 gtctcagggt gcagcaggcc cttatagtaa caactttgcg caggtcagca tccagttcaa
72421 tttctggagg cagcattctg tcttcagcct ttggagttcc aggaacaagg gcaggttccc
72481 caagtccttc ggaattcatg gcatagatgc ggatcttata ttcctgattc tctgtgaggc
72541 cagtgatagt atacgaagtt gccttgagtc ctgctggtgg agtgacaatc tgccattcat
72601 cttcctctgg cagggcaatc tcaaccatat acccagtgat ttctgaacca ccatcataga
72661 taggtttatt ccaagcgatt gaaatggatg atctgcttgt atccagaaca cgtgggttac
72721 ctggtggtcc aggtttaaac acagtgtcac aagccttgta aaatggactg gtaggacttg
72781 gtgcactaat tccagcagca ttttcagcca taatcctgaa ctcatattca tgtccttctg
72841 tcagtccaga cactttatat cttaaatcag taagagtttt tttgttgcat ttaatccagc
72901 gttggccagc tttatcacgc cgttccacaa tataattgat gatttcactt ccaccatcag 72961 aatcaggatg tccccagcag acaaccatcg aatctttagt aatagttgtc acttcagggt
73021 ttttgggcgg atcagggggt ccataaggat tcactgcaag cacaggctct gattccagtg
73081 gctctcccac tccatattta tttacagcca tgacacggaa tatgtattca ttgcctttca
73141 agagtttagt gacctttagc tttgttactt ggacttctga ggctacattt gtccatgcca
73201 atctgctggt ttcacgtttc tgtactatgt aatgatcaat tttggcacct ccatcatcca
73261 gtggagggaa ccatgatagt acacactttt ctgatgtcac ttcagttaca gccaaaggtc
73321 cttcaggtgg gcctggtctg tcaagaactt tgacattgaa aatgtgttta gcaaagccac
73381 caggattagt cgctgtaagg gtataggcac cactatccct tcttgttgaa' tctttgttta
73441 ccagattagt agagaaatct gcaattttta tttctaactt tgctgtgcct tccagctctt
73501 ttccatcttt gctccattcc attgttggag gtgggcggcc tgaaacatca gcttccagtc
73561 tgaatgcttc acctgctttt aatataaccg tgtccttaaa tttaacatcc acctttatct
73621 ttggtgcctc aacatcatcc ctgcaagtga tagcatcaga tggctcagat ggtggactga
73681 tggcacctgc agcatttttg gcgatcacac ggaattcata tgcagcatct tctgttaggc
73741 cactgactgt aaattcattc tccaaaatgt tgctgaagtt ggccttcagc caccgtccat
73801 taggaaggtc tctcttttca acgatataac tggtaatttt aaagccccca gtatattcag
73861 gcttagccca tttaagtgtt actgtgtgtc ttgtaatatt tagaggtact ggttttccag
73921 gtgggtcaat gggatccaga gccaacatag gttctgatgg cttgcttggc ttacttttgc
73981 ctgccatgtt ttctgcaatc acccggaact cataagcaat accatctgta agtccacttg
74041 atttgaaaat gttgcctggt actaaagctt tgctcacagt ctgccagaga ataccatttc
74101 gttcttttct ttcaacatga tatcctaaaa tggggcttcc accatcagaa agtggctcat
74161 gccagctaat tgtcattgaa tccttggtaa ctgcagttac ctgaggggta ccaggaggtc
74221 caggaacctt aaatggatag ttggcaacta tgcatgctga tgtgatgcct ggtccaactc
74281 catatctatt ctgagctttt acacggaact gatactctaa tccagtagta aggcgggtgg
74341 ctttataggt agtacgtata acggtggttg ctaactcaac ccaggtagta ctgtcagtct
74401 gccgcatttc cactacatag ttgcttattg gtacacoacc atcgttctca ggtgggtccc
74461 aagagaaggt tacaaaatca gatgaaactt catcaaattt gattggtcca gtaggtggcc
74521 ctgggatatc atggacttga atggtgatga catcaccaac ctctcctaca atgttccttg
74581 ctgttaatgg atagggccca ctatcacttc tgacacactc attgatattt aaaatggttg
74641 aagtcgctgt ggtttcaaaa ttaactctct gtgtctgttt aagaatttgg tctccttttt
74701 tccatgtcac tgtgggcttc ggtcgaccga gcactggaat ttcaactttg atgttgtcac
74761 cagctttggc aatgaccagt ttctgataga tgccacggag atccagctct ggaagcattg
74821 tctgctcctt gacaatgacg ggtctgcttt ctctaggggc acttctcccc gcgctgttca
74881 ctgccatcac ttggaaggta tattcctctc cttcagttag attcctcaca acacattcta
74941 accctttcac ggttgtgatg tgggtccact ggtcagagcc ttttctttgg gcttcaatca
75001 catagccagt gatcttgctg ccaccatcgt gtttgggctt aggccatgcc aggctgacgg
75061 tgctcttagt tatgtccatg atgttaaggc tgtctggtgg agatggtgct tcagaggctt
75121 ttacgggctc tgtagtttct gttggctcac caattccata ttcattctct gccatcactc
75181 tgaagaaata ttcacaccct tcagacaagc cggtaacttt atatgtgcat ttatggcact
75241 tagtggtggc tgtggaataa gatttccgtg ttgcttcacg tttctctaca atgtagtttg
75301 ttatacgtga gcctccatct atcagaggga ggtcccagtg cagggtgaca ctgtcctttg
75361 tgatgtctgt aggccgcagg ttgaggactg ggcctggcgt gtccaagact ctgacgttca
75421 caaagccact tttcttccca gccgggtttt caatggtcat gacaaattta ccggtatcat
75481 atctgttaca ttctgggata atcagaagag taaatgattc cgtattttca atgttggctc
75541 ggtttttcag gttgatgtta tctttggtcc atgtcacttc aggagcagga cgacctttaa
75601 ttggcacaaa tatcctaata ctgagtcctg ctctaacaac aagtgttctt cgaagctcgg
75661 catctagtto aaaatcagga gccatctccc gttctacgat ttcaacatct ggaatcaccg
75721 ctggctcccc aacacctgca tcgttgatgg cactgattct gaagttgtat ttttctttag
75781 tctgaagatc aggaacaacg aactgagtga ttctgagggc ggttcctgtg gtatctttta
75841 tccaggcctc gtctcctact ttttgatgct ccacgacata gccagtgatt tcaagtccac
75901 catcataatg aggcttgccc catgccaaag aagcagattt cttggtagta tcagtgacat
75961 gcggatttga aggtggtcct ggaggatctg agaaagaaac aaagacacaa aagtatatat
76021 tcagagtttg gcttttgggt aatttagata aaatattggc actctggaat gaacggtgtt
76081 gaaaaagaca aatactaaca tgctgcatct ttcatcagaa cagaatctga agcctcactg
76141 ggtggaccaa ttcctgctct gttttctgca ctgacacgga attcgtaggt gcttccttct
76201 tgcagtcctg ttactttgca cctgagatcg gaaactggtg tttttattgc tctcacccat
76261 cgcaggcttt tcttttctct cctttctaca tgatatcctg taatttcgct gccaccatca
76321 tccactggcc ttttccagct gacagtggca gtgttcttag tgacatttga tacctctggt
76381 ttttcaggaR ggccaggggg acctgaaaag gaagcaaatt tattagaaat ccatgatttc
76441 ctaaactctg ctataaatgt ttccatgtca attccctcac atgctctaca taccaaatct
76501 gtctaccatt ttgacaggtt cagactgtac aggttctcct ttgccatagt ggtttacagc
76561 tgagacccgg aagatgtact catttccttg gataagtttg gttgccacat gcctgcaaga
76621 ctgaatatct tcagaaacca ctgtccacaa aagtcggctg gtttctctct tttcaagtat
76681 ataggactta attggtgagc cgccatcttc caagggaggt gtccatgtaa gtgttgcttt
76741 ttcagcagaa acattactga tttcaacagg acctggggga ccaggtacat caaggactgt
76801 taccttcaca tgttccacct tcgtgccaaa aggattggta gcagtgatgg tatattcacc
76861 cgcatctttt ctagtggcat acttgatagt aagcatggaa gatgttgggg ttgaagttat 76921 ctgagtgata tctgatggtc taatgtcttt tcctgccttg gaccaacttg attttggaag 76981 gggtttgcca agaatgctaa tggcattcaa aacaatggta tcccctgctt taattgttag 77041 cccatctttt attgtgggat caaggacaat tgttggtgcc tctgcaaaga aaaaaataca 77101 ttttaatcag aaaaggaagg aggcttaatt tgctttaaaa aaaaaagtac ataaaaagta 77161 aaatggacct accgtattca tccttgcaaa taatggtttc tgtactttct gatggagcac 77221 tgatagcacc tgctgtattc tttgctctaa ttctgaattc atattttcca ccctcaacaa 77281 ggtcagttac agtaaaggca cattctggga cattaacatg gttggctttc atccaagact 77341 tcgaaggtag atctcgcttc tccactatat atccagttaa cttatgacca ccgtcatatt 77401 taggttcagt ccaaatgaga gtcactgaat tccttgttat gttaataacc tcaggttttc 77461 caggaggatc taaaacataa aagcaaaacc agtcaaacaa ataccagttt tcttcatgta 77521 aaacatgcac ctgggttttt cttcatataa tgacttacca attgggtcca gtgccacaac 77581 aggctctgat ggtaggcttg gcttgcccac acctgctaaa ttgattgcca taactcggaa 77641 ttcatattca agaccttcag ttaatcctgt cactttaaag tctctcatcc ttatcggagt 77701 cttgttagct ctcttccaca aaaggctgtt tctttccttg aactcgagat gataccctac 77761 aaaagaccca gggatgtatc aagtataaat gcagcttgat tttacaatat atatgtgttg 77821 cccaaattat ttatacatat ccatatatat tgaacaagta gtcattcttg ctagtatcta 77881 tacttttatt acccaagcat agtgccattt tttttttcct cttggaatat gcatagttgt 77941 tgtctttttt ggtgaacaat tttaacttac aaaaatagat attataatta agaaatactt 78001 tattaactat tacattagga tattttcaat aaaattaaac attaaccata tgtactgaaa 78061 Kaaaaacaca tttctgtata agcagataat gttatcgata agcacatgtt caactgttct 78121 cagggaaata tttgtgagga atcttgatgt aaaagctgta ggataagaac aaacaaaata 78181 cctgtaattg gagaaccacc agttttcttg gggtcagtcc aagttagaga tactgaatcg 78241 tgtctgacat ccacaatatt gggaggtggg ggagcatcag gcacatctag aaaaaagtag 78301 ataatgcaag atttataata agaagcctcc tcaaaaccgt gttttgcttt acgtcttctg 78361 aatttaagac acttaccaaa tggatgtctc gca'acaattg gctccgattt caggccttcc 78421 cctacaccat atttattttc ggcactgacc ctgaaggtat attccatgcc ctcatgaagt 78481 ctggttactc taaaggtggt tttctggaca gctgaggcgc acgtcaccca cttgttgttc 78541 acagaatccc tcttctctag gatatagttg gtgatttcag aacctccatc atcctttgga 78601 ggagcccact ttaaggtcat ggcttctgct gtgacttcat caaatttgat tggtccagtg 78661 gggatgccag gcttgccaac aacctttatg gagatagttc cttccttggt gccagcaaca 78721 ttcttaagtg tgattgtgta ttttccagca tcagattttt ggcaatcgta cactataaga 78781 gttgtgttaa ccgcagatga ctcaacactg actctagtgt cagttgctag aggatcttcc 78841 cctttcttcc aggagactga tggagctggc ttgcctttta tggggatctt aagccggaag 78901 ttgctgtttt ctttagccaa gtagcacaaa tcaggtagac cctttaagtc aagatcagga 78961 gccactgtaa aatagcagag Rtaaattact gttattttta aggccaggcg attaactttt 79021 tcaaatagtt ctagctttta acggattttt agataagcta gtgagtggga cacaagagtg 79081 cactttcgat gacaataaag gaaaaatgaa gatgtgtgca tagagcctta ccRacatcat 79141 cccttgccac aacagtgatt tcgcttgggg ttccttctcc attttcattc tcagcactca 79201 ctctgaaggt atattccttc ccttcagtca aatcttttgc agagtactgt aggcttaagg 79261 atttcataac tcgttgccac ttattttctt cagtcaggaa atcaactaca tatccaataa 79321 tccgacttcc accatcactg tgaggctttt tccagccaat gctacaggat gacttagata 79381 cagacctcac tttcaggtcc acaactggtc caggagtttc taaagcaaag gagaaatgat 79441 aagtgtaagc cccatataac aaaggaagga tataactcaa aatgatggga tgatggttca 79501 tttgcttacg ggaagctttg acagcatcac gagtttcacc gggatcacca atgccatact 79561 cattttcagc aaacactcgg aagaagtagg attttccctc ctccaaatta gctactctga 79621 agcttgtttt ggagcactca gttgtcactg tagaccagga tttcctctct gcatcacgtt 79681 tttgtaccac atagtttatg atggggcttc cgccatcaat aatgggaggc tcccaggtaa 79741 cataagcaga gtctttggat atttccttaa cagtcacatt gaYaggtggc ccaggagtat 79801 ctggataaat agtaggtaaa taagaaatga atgtgtaaaa aatacataag ctattttaaa 79861 atatatatga ttcttttttg aaatagactt accaagcact ttcacaacaa Yggtatattc 79921 ctttttacca cagctgttct ccagggttaa gatatatttt cctgcatcat atttgttcac 79981 attttcacag cgcaagaaag tgtcaaagtc agttgacttt atgtccagtc caatcctgtc 80041 tcttagattg acatttggtt tagaccaagt aatctttgga ggtgggcgtc cttttactgg 80101 tacatatagt ctcatagtaa ctccagcacg taatatgagt gtcttcctta agtccgcatc 80161 aagttctccc tcaggtggaa ctgtttaatt ttgggtgaag aagttaaata aaaatttaat 80221 agtcaaatgt atttccaggg actggccttg tcactcctaa aatacattta ttttctagag 80281 ctgatttctt gttctttaaa acttccttaa tacaatcctc aaaatcattt ttctgttctg 80341 tactaattgt tgatctttcc attttccact atgctttagg cttaaccagg ttttcccccc 80401 ggaggatctg tacctcctct ttcatgagcc ttatgtactc tgaaatgcta catatattaa 80461 aaggaaagta gacgattgat ttttggctcc ctttcattct tgttttttcc tttctatatt 80521 ccatgaaggt attctgccac atattctagc tctctttatt atttcatagt aggaagaaaa 80581 gtacctggag ggtgacatat aatatcaagt aattcaaata aaggggaaat tatcatggag 80641 gataagcaaa tagaagaaat aagaagaagg gaaattaaga tggagagccc accagtcaca 80701 tacttaggga gatggtggat tggaaaacag aaaccctaag aatagtaaat agctcactac 80761 ttagttaaaa ggcctcttta ttcctaaaat aaaattctta cattccaata aaattctctc 80821 atttcatgtc ttttgaagac aggaaattct tatatatatg aaaagctttt caaaaccaca 80881 aatcatgtgc atttgatttg cctgttccac aataccgtaa agtacccacc cagctctcct
80941 aaaaagtact cttggagtta ccagctctac accttgttaa taaattaatc tacctaaggg
81001 agttctagtc tccctcttag aatatgatta ataggaaaag ccttatgtac tccccctggt
81061 aatacttact taagatgtcc ttgggatagt gtttatctgg cacatcactg gggtcactgt
81121 atccaatctt attaacggca tacacacgga attgatattg tgtgtcttcc atcaggccag
81181 taacctcaaa gcgggttttc tgtagattct gtggtaagtt gcacctcacc cagttatcgg
81241 agtcagcccg ttttacttca acgagataac caatgatagg gctgccgccg tcataggctg
81301 gcttgcccca agatagactc acagagctgc gagttgtatc atatacttta gggaaagccg
81361 gtgggccagg aggatctgag aataaataat gataggaaat tttcatgaaa acttccttcc
81421 tgggtgttta atgctgcttt taacacagga tatggaactc gttcatgaac acttacactg
81481 agggtcccga gcataagcgg ccttggatgc gtcactgggt ttgcctggtc cagctttatt
81541 tatagctgta acacggaact catattcggt accttcttga agacctgttg cttttaatgt
81601 tctttctata ataggttttc tgttgacctt agtccagtta acagcctggg tttcccgctt
81661 ttcaagcaaa tagccagtga tgggtgagcc cccatcatct gctggtggct tccagctgac
81721 tgtcatgtga tctttggtta tgttgctaat aacaggagga tcacagcgtc ctggtgggtc
81781 tgcagaaatt gattgaaaag ttaatttccc aggctaaaag ataattttaa aaaataaaat
81841 gaaaaaccac cgggaaatgt tcctaccata tgggtttttg gcaattgctg gttcagtgaa
81901 gactggatcg cctactccat atttattttc agcacaaata cggaactgat actcatggcc
81961 ctctataagt ttctccacgc tgcagctggt gataggcaca gttgcagaga cttgagccca
82021 gttgggcctg cttgtttcac gtttgtcaac aatatagttg gtgatttctg agcctccatc
82081 ttcaagaggc ggttccoaag aaagcatagc acgatctgaa tacattttgt tgattttaac
82141 agatgctgga ggacccggtt tatctgaagg aataaggaag aagagtgaat atgtggaatg
82201 gggaatgagt atagagtaga ggtctggaga cctgggctgc agtctttcta ttgtcaattt
82261 cctatatgac ctaggaggtt agttaatttt ttagaatctt agtttcttca cttgtaaaat
82321 taggtaattg aaatatgtga tttctaaagc tctttttaac tctaaaacgt ggttctgtca
82381 taagaataat tctgtgcata ggaaatacac attagaatat gaatgtcttc tcccacatta
82441 ttctgtaaat cctatttaca tcccattatc caaagtaaaa tatgggattc caatgaagtt
82501 tattagttac ctaacacttt aagcttaatg gtggctgatt tagaaccact tgaattttca
82561 gcagtaatgg tatagtctcc tgagtccttc cggttcacgc tgaatagctc caaggtgcac
82621 agattccgct gcatggccat ctttatgcct tctgctggtt ttagcagtgt gccatctttt
82681 ttccaagaaa ctgttggcat cggtttacca aaaacagtag catccaagca gacattagtt
82741 ccagctttca cagtaagcaa agatttcata gccacactca ggtctatcct gggagggact
82801 ggaaagaaat aagataaagg acttaatgta tgcaaagcta tattatgtaa tccttattaa
82861 taatagtggg aaattcatat gctgcttctt tcaagaatgg aattttagtg ttatttcatt
82921 ttgtttattt ttaatagaca aataataact gcactattga ataactattg aaattatata
82981 gttgccacag ttgtgtatct tgctttttaa tttagcatgt tattatgatt ctccataatc
83041 ataatgtttg tgtctatata caaaacatca ttttaatttt ccttttttcc ttaggtgtat
83101 atttagtttt ttattaggaa aaatgaccat catgaatgct gttacatctt tgcctataat
83161 actcagcaga atcttaccat tttctgcgag gatagtcact ggatcagaag gttcagaagg
83221 tgggctaatg tttaccgcgg tcctggcaat agctctaaat tgatactgag ctttctcttc
83281 taggccagta gctgtgtact cttcctgggg aatctggtga ggtgcagtat tgcaccgtac
83341 ccatttatca ccaggaagtt tgcaagcttc tacgaaatag ccaataattt tactgcctcc
83401 atcatgcttt ggacgagccc agatcagaga tacagtactc ttggtgacat caataacctc
83461 aggtttccca ggaggatcta aaacaaaaag aggtacactc accatttatc ttaccagcag
83521 aagtttaaac ttccatattt cacattatca tccctgctgc taagtaacaa gctcaacagg
83581 aagacaatta tcccatttta ataaccgtcc aactagtatg cacttttgag tccttgctgt
83641 atgctttaaa ggaaattata tgtccttttc tttttttaat atgtcatctt cattgtaaga
83701 gatagaacat attcttaaat gggagttgta atcttcagat ctctactact ctctgtgtct
83761 tggagtccaa atcttagact ccgagattta aatgtgcctc cataatacta aaacaacaac
83821 aacaataaaa aaaccccaaa acttaccaac tggcattctt gcagttacat attctgtggg
83881 tttgctgggt gggctggatc cagctttgtt tagggcatag attctgaatg aatactcaag
83941 accttctaca aggccagcac aaggatattc agttgaccgg acaagagtat cattggcttt
84001 cacccacagc aaactgtttc tttccttgcg ttcaatcaga taaccaataa tggggctccc
84061 tccatcacta tctggtctgt cccaagcaac aaaaatacag tccttgttga ctttggtgac
84121 tcgtgcattc tttggttcac taggaacacc tggagatgaa gacaaggaag atgtcagttt
84181 ctacattaag taacaaacta gaaagaaaac aacttttttt ctccttcaca aaaacatacc
84241 aaatgggaac tgcgcaacca tctttggaga tgtgagaggc tctgaaatgc caaatcggtt
84301 ttcagcacgg acccggaaga tgtactcctg gcctgggatc agctttccaa ccctgcagga
84361 agtttttgtg actgaagcta gagccgtgac ccagtcacct cggcttacat cacacttctc
84421 cactatataa ttggtgatgt tactgcctcc gtcctccaga gggatgtgcc atgacaggga
84481 gcaagcatca gcgtctatat cagaaatgtc aaatggaggc tgaggggggc cgggggcatc
84541 tacatgaacc aagaggaaag aaatgtaaga acaaggattt gatacgtaaa aatatttctg
84601 agcttcatat ttagatggcc atttgtctaa tacttaactt acccatgact acaactttga
84661 ttttctgagt gtctgtccca gaactgttct ctgctgtgag gctgtagtaa ccactgtctt
84721 tcctagtcac atcttttatg atcagaattg aagagctgtc tgctttatgc actgccaggt
84781 ggctggatgt gacaacttca tcttctcctt ttttccattt acaggtggga tgaggctttc 84841 catacacatg ggcttcaatt cggagttttt tcccagcttt gatagttatt tgctctggca
84901 taaggatctt tggtggcact gaaagtaaaa tgaaaagata ttaattttgg gaaggtggat
84961 aagtttcttc tggtggagag ctatactggt gcaaattaat gcttgtatta ccaccaattt
85021 tttttaatat atactttaag ttctgggata catatgcaga atgtgcaggc ttgttacata
85081 ggtatacatg tgccatggtg atttgctgca cccatcaaca catcgtctac attaggtatt
85141 ctcctaatgc tatccttccc ctagcctccc aacccctgac aggccccagt gtgtgatgct
85201 cccctccctg tgtccatgtg ttttcattgt tcagctccca cttatgagtg agaacatgtg
85261 gtgtttggtt ttctgttctt gtgtttgctg agaatgatgg tttccagctt catccatgtc
85321 cctgcaaagg acatgaactc atcctttttt atggctgcgt agtatcctgt ggtgtaaatg
85381 tggtgccaca ttttctttat ccagtctgtc attgatgggc atttgggttg gttccaagtc
85441 tttgctattg tgaacagtgc cacaataaac atacatgtgc atgtgtcttt atagtagaat
85501 gatttataat cctttgggta tatacccagt aatgagattg ctgggtcaaa tggtatttct
85561 ggttctagat ccttgaggaa tcgccacact gtcttccata atggttgaac taatttacac
85621 tcccaccaac agtgtaaaag ctttcctatt tctccacatc ctctctagca cctgttgttt
85681 cctgactttt tagtgatcac cattctaact ggtgtgagat ggtatctcat tgtggttttg
85741 atttgcattt ctctagtgac cagtggtgat gagcttttct tcatatgttt gttgtacagc
85801 caatattttt aaaaacacag aatagggtag aaaacataag aatgcacatg ataataaatg
85861 ggtattgttt caggaaatgt tttttagtta tatatgtgtt tgtataagtt tatttactgt
85921 gtgtttttgg cccactcatg tgtttgaaag ccactgttct ctactgtttt tcaagaattt
85981 ctagagctag attcctcagg gagaaatgtc tacatataaa agaagaaatt ctaataaact
86041 taccacatga cataaatgaa gcaaagacta cttacacagt tgttctttgg cttcatacac
86101 tccttcagct tctcttggca aactgactcc aacagcattt ctggccgcta ctctaaattt
86161 gtatttcttt ccttccttta ggccagtgac aacaaggctc agatctttta ccactgagta
86221 ctctgtccag cgatctgcag gctgctcttc ttctctgtaa ctaRtgatgt agccatcgat
86281 tttagcacct ccatcacgta ggggaggtaa ccaggctaag gtggcactgt tcttggtcat
86341 ttcagtcact tctaatttcc ttgggggatc cggctcacct agaagaaaaa cataatttag
86401 aagattacct aggtaaccta atcaaatccc ctttgttaat gtacataaga gttttagtat
86461 ggatttgctc caatgtacat agaacctgta gttcagtaca ttaRaagcaa aacatataag
86521 atgagacaga tcaaaaaagt gtttcatgag tgagatagat atttattaca ctatttcggt
86581 ctctacaaat gaaatctctt tcagaagtgg attaaaatgg tattagtatc ttgaccttgc
86641 taaaatagga gactggggtt aaaaggagga agaaagcata atacttactt agaggatctg
86701 atgcaagcac tggttttggg acatctgttg ggacgccagg gccatattcg ttgacagcag
86761 ttactctgaa gtaatactca ttcccaggga caagattggt tacatggaag cttgttttct
86821 taacttctgg ggtaacggtc gaccatgtct ttctgtctgc ctcacgtttc tccacgatat
86881 aatgtgtcac ttggctccca ccgtcgtttt caggaggggc ccaggacaca tggcatgatg
86941 ttttagtgac atctgaaact tttaaatcag acacaggccc aggagtgtct gtaaagaatc
87001 ataaaatcag atatacatgt ctcctcagca tccctattaa caaattcatt tttgaagtgg
87061 gagggagaag cattttagta acccacctaa tactctgaca tttacgaata cagccttttc
87121 tcctgctggg ttcacaagtg ttaaggaata ttttcctgag tcatcacggg tagaattggg
87181 gatgacaagg aaggccatag tgtcaaccag atcaacttgt ccttttctga ccacattatc
87241 aatgccaact tttcgccaag tgactttagg ggctggtcgt cctctcacta tagcaaagag
87301 acgaataggg catcctgctc tcactatgac cagtttcctc atgctggcat ccaaatcaat
87361 ctccggaggt tctgcaaatg acattaaggt cattaattga tttttcagaa tgtggggaaa
87421 tcataagaaa ttaagtgtga atgaatcaca tgctaaggga aggacttacc tagtatttct
87481 ttaggtttga tagcttcctt tagctctgca gggcgcccaa taccaacttg gttttgggca
87541 cacaccctga actcatattc ctggttttca tccaagctgg taacagtgaa ttccttgcgt
87601 acaagctgtg ctgcagcatt acaccgtttc cagccttcat caggagacgc atctgctatt
87661 tttggtctca tttccacaac atatccaatg atcggtgcac caccatcata gactggtttt
87721 ccccacccaa gagttatgga atgtttggtt gtatcaacca ctctgaaatt ggttggtgga
87781 ccaggtggct ctgaaagtaa aatatacata tagttaacta ctactagtga tacttcaatg
87841 tgtaatttta tatagcctat tctcagttaa tcaaatatag gtcatccaat ttttcaatta
87901 gtgtcctctt tattttcctt cataaaaatg agaatctact attgtttgta ccttttaggt
87961 attttggtaa aaggtctatt tggaaaaata tttgctaaat acagtttgga tgttacagat
88021 ttagatgtta cattaactta tttatcgaat acttctgtgc ttgagattaa gagttgctgt
88081 aatatcagaa aaaacaagga catcctacct atggggtctt ttgccaccac cggtcttgaa
88141 ggcaagcttg gttctccaat tccaatttca ttttctgcct tgacacggaa ctgatattca
88201 tggtctggga ggagattctg tactttcata cgtctctcag ggattgcact cttgttgaca
88261 gggacccatc gtgttgaatg cttttccttt ttctccaaaa agtatccagt tatagacttt
88321 ccaccatcat attcaggtgg attccaaacc acagtcatct cttctttgct tactttagtg
88381 acttctgggg gatcagggcg accaggttta tcatacttgg ttttggctat gactggttta
88441 ctttctgttg gaggccctgt gcctatttta ttttctgcac ggactctgaa aatatattca
88501 tttccttcta tgagacgtgt tactgtagta ccaggtgtca agacagtagc agaataggta
88561 gaccaaacca ttcgctttgt ctcacatttt tctagaatat aattttggat ttcacagcca
88621 ccatcatctt ctggtggatc ccagcaaaca gtacacctat cactggacac atcaacaatt
88681 ttcagatttc tcacaggacc aggcttatct aaaacattga cagtggcata ggccacaaaa
88741 ctgccagccg tgttagttgc cgtaactaca tatttacccc catcacttcg ctttgcttta 88801 gtaagagaaa atttagatga atcagcacgg gtatcaatct tgacccttgg tgatcttgtt
88861 aagtctgtag cgtctttgtc cttggtccat gcaacttctg ggaatggttt gcctctaacc
88921 cctgcctcaa gcctaatggt gtcccctgct ttgacagtta ggaccccact taatttcaga
88981 tcaagtactg gtttttgtaa gtcttctttc acaacaactt cctctgtctt cacccagtca
89041 ctttccccac cttcattctt tgtttgcact ctgaactcat aaatctggtt ttcaacacat
89101 ctgtcaacca tgtagtgagt ttctttaatg cttcctttat gcactctttc ccagtcatcg
89161 gagcccttaa gccttctctc aacatgatat gacagatttg gactgccacc atcatagtca
89221 ggcctccgcc actttagRta gacaaatgtc tttcctttat ctgcaatgtg aaggttttca
89281 ggctcacctg gtctgtcaat agggttaata gccagaatgg gagtttttgt ttcgatggtt
89341 ggcccaacac ctactttatt ctctgcacaa actcggaaat agtattcatt gttggctaaa
89401 aggttggcct tgattaatcg tttagtgaca tctgatgcaa caattgacca gcctttctgg
89461 tttggtttgc gatattctac tatgaagttt gttatttctg agccaccatt atctagtggg
89521 ttttcccaag aaattgtaca gttctctttg gttacattgg taaccttcaa attctggcaa
89581 ggcccaggtc tgtcaaggac gttaacgatg gctgaacctt gggcatgtcc actgctgttc
89641 ttagctgaga tgatatactt gccatgatca gctctaacag cttctttaat ttgtaactca
89701 acacgaggta gatcctgaat aaggtccacc ctcttttctc ggaccaatac tttgccttcc
89761 ttagtccaag ttatgtctgg ttcaggtctg cctttgactc gagctagaat gcggatagtt
89821 tggcctactc tcacggtaat aacatcacga caagtaacat caagttctac ttctggagga
89881 tgaaggatat cttttgcaat cacagattct gctagttctc ttggctcacc ctctcctaca
89941 atattagctg cctttatacg gaatctgtac tcatttcctt caattagtcc aggtacccta
90001 aaggcacatt gcctaatgag ttcatcttta ttaatcctgt tccattgtgc Ygtgccaggt
90061 ttctgacatt ccactacata tcctagaatg gggctaccac catcactgag aggctttgtc
9-0121 cacaccaaat cagctgtttc tctgctcttg tctttcagtt taggattaat aggtggtcca
90181 ggtggtttga tgggccggca agcttttatt ggatcagaac ttggggatgg tttgcttagt
90241 cctgcaagat tttcagcaaa aactctaaac tcatatgtat tt'ccttcata gagtccagtc
90301 actctgaact tcaggtcagc gataggtgtt ttgttgaccc tcacccattt gccagttact
90361 tctcgacgtt ccatatagta gccagttatt ggactgccac catcagattt tggcagggtc
90421 caagacactg ttgctgcatt ttcagtaatg tcagtaacca ctggtttacc aggaggagac
90481 ggaggactaa atttatgctt agcaacagta ggtgtggagt caaggggagg accaacacct
90541 atcttattct ctgctctaac tcggaaaaca tattcacagc ccttctgcag atgtgggatt
90601 ttcagctttg tcttactgct tccggaagag acaacacccc acgtgtcttt ccttgtatct
90661 tgtttctcaa caatataatt tatcacaggg cttcctccat catccttagg tggctgccat
90721 gagatagtca tgtgttcagg agtaacatcc agaatattaa taggacctgt tggtggccca
90781 ggaacatcaa gaacagtaag atgtacggct actgttttgc taccggctgc attggaaact
90841 gtgatttgat attccccagt gtctctcctt aagcagttct taatggtaag tactgatgag
90901 aagttgtctg tttcaactgt gtagtgctca tcggttttaa tctcactccc atcggttgtc
90961 cactttgcag taggaacagg cacacctctt ataatagcag ggaatctgac tgtggttcca
91021 gcctttacca caagaccttc aattaatttc acatctagct ccacggatgg aggcactgaa
91081 aagtaaacat gaaaaaatta gattttgatt ttcacctata tgaataattt taaaaagaaa
91141 taaggtaata ctgctagtcc aaaaattacc tagtttttct tgacattcta tcgggatagt
91201 tgtgtcaggg agaccaagac ccacaatgtt ttcagctttt acacggaatc tataggtctt
91261 tccttgttgt agtccagtaa ccacacactc taagtttgtc acagtcttaa atttaatcca
91321 gtcctgggtg ccttcttctt gatattctac caaatatcca gtgattggag aaccaccatc
91381 acgatccggc ttattccaga ctagggagac ttcagtcttg tcaacatcta catggtgcag
91441 gtccttgggt ggccctgggg gatcttttca aagaagaagt tatgatgaaa aagtaatatt
91501 cttaaagaca gtcaaacaat agttttgtat tcagagaaag caacttacgg agaggatcca
91561 aagccttgat tggttttggt gtttcaacaa aaggaccacg tccatactgg ttctccgcag
91621 ctactcggaa gaggtactgg ttgccttcat tcagatgctt agcgaagtga ctctttttct
91681 ttgatgtagc tgagagaggt gaccactggg cgctggcaac atctctcctc tcaaccacat
91741 aattggtaat aacagaacca ccatcatcca gtggttcttt ccaagtaagg taacatgaat
91801 ctttcctaat ttcactgact tccagatctc taggtggccc aggtttatct aaaaagtgtt
91861 aaataacagt ttgataagta attttatcca tataagagct caaaattatc aaaatttatt
91921 tttaatggcc taagtagtaa aattcttacc taatacaagt acttttactg agacagtttt
91981 tgaaccagct ggattctcca ctgttaaaga ataaattcca ccatcttcat gggcagcatt
92041 acgaatttca agcttgctac caactggagt cacatcaatt cttgctttag ttggagcatc
92101 tctgtcttct tttttccaag ttacttttgg gaatggcact cctttgatga tggcactaag
92161 tcttagagta tcaccaactt taatgtgttg ttctcttgcc atgttggcat caagaatcaa
92221 ctcagggggt tctagaataa agagaacaga acactcagat ttgatccata atgcagatta
92281 caattaaaca catacaatca gacatctatt ttccttgcaa acacaagtga gagcatttta
92341 ctcaccaagc ctgtctttta ctaggactgg ctccggaaca tgagctggat ctgattcacc
92401 agctgcattg actgctaaca ctctaaactt ataggtttga ccgtcccgaa gaccggtgac
92461 tttatattta gtttcaggac atgactcagg ggtgtgattg gctcttctcc actcttcatc
92521 tccaactttc tggtactcaa caatataacc cagaatcttg ctcccaccat catgacgtgg
92581 tggctgccaa gttagatcga ctgaattgca tgttgtatct attgcttcag gattaacagg
92641 tggacttggt ttagctaaaa aataaaagta aaaaacgaat tagcatatag ctgaaaacaa
92701 agttagataa catgaaaact gaataaatcc attaatacta taccaattgg atctttagca 23933
92761 aagactggtt tggatggtgg gcttggatct ccaataccaa tttcattttc tgcagaaacc
92821 cggaattcat actgacatcc ttctaRaaga tcaggaaccc taaatttagt gtatggatga
92881 ataggatctt tggtaactct agcccatcgt ttagacatag tttctctctt ttccaggatg
92941 tagtttgtga tgggttttcc tccatcatgt ggcttattcc aggttactaa tgcagagtct
93001 ttggtaactt ctgtaacaat tggctgatca ggtgcatcag gaacccctgt aacaaatgtt
93061 ggaaaatgcg ttagaaattt atttctttat attagtttta tcaagcattg aatcactaaa
93121 aagaaacata atgcatactg aaacgatctt tggctttcat tgaatcagac accagaggat
93181 cacttattcc atacagattt tcagcatgta tccggaaaat ataatctttt cct'tcaagta
93241 gtttagaaac tttgcatgtt gttttagcac ttgcagatgt cactggcatc cagacgtctt
93301 tacccacttc cttcttctca ataatataat tggtgatttt actgcctcca tcatctaaag
93361 gaggcttcca agagataacc atgtaatctt tggtcacctc atcaaaacta actggtccta
93421 ctggtggtcc aggaYggtct gcagaaaaaa aaaatcatgg cacaaaatgt tattgccatt
93481 tctgcttttg aaagaacaga agatctattc tttccactaa ataagactta ccaacaacat
93541 taacttgaca gaaacctttc ctagagcctg tactgttctc cacaaccaca cagtatttgc
93601 cggaatctga acgtttggcc ttgatcttct ctaaagcaag tgttgctggt gtagtcttta
93661 tatgagtgcg atcatcttcc agcacatcag cttcatcttt gaaccaggaa accttaggct
93721 ttggtttgcc tgagtaacgg ccagtgaggg caaaagcttc accaactcga atcgtgagct
93781 tatctctgaa gtcgaggtga agcgttgggg gtgctaaaat ttgtaattat aaaggcaagt
93841 catttttatt gatgtcttat tacaaatcta caaatgtgca agtttcagaa gtataaattg
93901 tagtagacac atacccagct catccttgca agtaattggt ttggtgcaaa atgatggttt
93961 gccttgtcca acaatgttga cagcactgac acggtactca taggtatcac cttcttttaa
94021 gcctttaaca gtgtattttc tgctaagcaa gttgtcattt gtaactttgt ggaacttctc
94081 agtcccaatg agccgacttt ctaggacata gttaataatt tctgacccac catcatactt
94141 aggaggattc caagtcaaag ttacagtatt tttagtaact tctttgactt ccaggtcttc
94201 aggacgctct ggtacagctg cgaatataag tataggaat't gtggtagaaa aaaatgtcac
94261 attaagaatg tagtaggatg tagtgaatat tccttttctg aagattgcat ttaaacatta
94321 aactctctgc tcccataaat agcaaaagag atctcatcat aaaactatta gagttaaaaa
94381 ggacccaata caattgaatt tcttcacttg acagatgagg aaatagaggt ttgttgaggc
94441 aaaataattt aggaaaggtc ttcagaattg gtagttttag aagtaggaaa ataattcagt
94501 ttctattaat gccattaagt gggaaattaa aaaaaaatct gtgaggtata gatggaagaa
94561 atttgataaa tgaactagga gtggtaatgc cagcactttc ggaggccgag gcaggtggat
94621 cacttgaggc caggagtttg agaccagcct ggccaacatg gcaaaaccct gcctctacta
94681 gaaatagaaa aattagctgg gcatggtggt gcatgtctgt aatcccagct actcgggaga
94741 ctgagacaca agaatcactt gaacctcaga ggtggaggtt gcagtgagcc aagatcatga
94801 cactatactc cagcctcggt aacagtaaga ctatgtgtca aaacaaaacc aaaaagaaaa
94861 aaaaaaacaa aaagaaattc aatgaattat acatatttgt aaaatctctt tcacaatcac
94921 tgtccaaata ctaccaaatc agatactgag tagttgtgtg ataactgctt gtcaagtgaa
94981 tgaaatgtac ggcatttata cacatatttt ttcaccatag tcttgtacta aatgagtaaa
95041 gaagtgatta agtatacatt tttttttttt tacttactta ttggctctct gataacaagt
95101 gcctctgatg tctcaacaaa tggacccatg ccaatactat tttcagccgc aattcggaaa
95161 aagtaggctt ttccttgaat gagaccctgg acagtagcat tttgtcgggt aactgtatat
95221 gtcactgggg tccatgctct gcggtcagat tcacgctttt caatgacata attggtgatt
95281 ggagagcctc catcatcttc tggagaaaac catgtcagtt tgcaggactc attggttagg
95341 ttgtgagcta ggaatggtgt tccaacggga cctggaacat ctggaaataa gaagaaaata
95401 attcaagctg tttgccttca atgaaagata atgctcaaca cttgtttttt taaaaggaga
95461 caaaaatgtt gttttgaaga aagtaaggtt ttgtgtctac taattgagtt agtttttcct
95521 tctgctatct agtcaataca aaaacagtca accacttttt tttttttttt ttttttttga
95581 gatgggagtc tcgctctgtc acccaggctg gagttcagtg gcacaatctc agctcactgc
95641 aacctccgct tcccaggttc aagcaattct cctgcctcag cctcccaagt agctgggact
95701 acaggcatgt gccaccatac caggctaatt tttgcatttt tagtagagac agggtttcac
95761 catgttggtc aggctggtct cgaactcctg acctcaagtg atccacctgc cttggcctcc
95821 caaagtcagg ggattacagg tgtgagccat agcatcaagc cccatatttg ttaatgaagt
95881 aggtttgcaa cagttattgg ttaccatggt tttagtagag gctgaaggga aaataaaaat
95941 gctttttaaa agcaatttgt gagttggaca ggagggagct gggaggcaag catgcagagg
96001 gatacaagag atgggtaaga gtggcatagt cagtttctat gtccagtttc ctctgcaggg
96061 gaaacggaga gagagatggt ttatttctgg atgaccagtg gatcaacaag cagcatagga
96121 ggccccaaat ttcagaatga ctttgaggag gttgcaaata attaacgtgg aaatgtacca
96181 gttctcaaat- taaagtaata accattaggc aaaagagctt taggtgcttg aaactaacct
96241 gaaagtttgt ggggctactg cttaatgaaa tatttttcct tatgccattc ttatattaat
96301 tatattcaac atagaactgg caattttcac ataaaaactg tggctggagt attgttgtgc
96361 ctagagacat gcttagaaag agccctgagt ggaaaggctt cgtgttctga gcaaatcact
96421 ggactaaagc ctccagtgag agccatgaaa aagcctcata gagttcaact ctatattcag
96481 tgactagaaa tgcaatattg ataaccaact atgatgcttc tcttcaatta gaatactgaa
96541 acccttcaaa caggtttttg cattattatg ataatttagc gataggggta gagtctcatg
96601 atgtgaaatg gtcagcactg gcaaattgtt aaacattcat gtacagttta aggaaagttg
96661 cagattgtta aacattcatg tacagtttaa ggaaagttgc ttctatgaaa actatataac 96721 aacataatag acactatggg ggcaagttct ataaaaaagc catatttatt ctgggccatg
96781 attcagaata catattcatg gccttctagc aatttgagaa ttgtgtacat gcactcttta
96841 ggttcactgg aaacatggat gcatgtcttc ttgttagcat ctgttttctc aattatataa
96901 tttgtaaact tacacccacc atacagaata cacatagaat aaaaaagtaa ggaggaaatt
96961 tttaaaaatt ctaataatgt tcaacatgat tatgggtgat ttttcttatt ttccaaaatg
97021 tttgttacac agcatacagc atagctttgt gtaaatataa taagaatgtt ggtttaaaaa
97081 gttgcacaga caaattgaaa gtatttacct aatacatcaa caataattgt cttctttctt
97141 tctccgcttc atttttggca agaagagaat agacgccttg atggctcctc tggcagttct
97201 tgatgaccat ggatgagcta atggctgtgg tctcaatggt ggcttcttga ggtaaggttc
97261 tttcattcat gttccaggtg acggttggag gaggctttcc agacacatag gcaatgattc
97321 ggatcacccc tccagcatgg acaacaattc tatctctgac actggcatct agctgaaggt
97381 caggtgaaac tggaagcaat tgaaaattac aaatgtgatt tttccccttc aatctgaaac
97441 ataaatactg atggcataag gaaggattga taattaccaa gtctgtcctt catttcaatg
97501 acatctgtga cctctccagg ttctccaatg ccagcaatgt tgactgctct aactctaaat
97561 ttgtagaatg ctccttcctt taatcctgtc acaacaagct ttgttcctct cacttcttta
97621 tctttaccct ggggagaaat catagacatt ttataattag aatagttctt tgtagcttct
97681 tgctttaatg gcttcctaaa gcatcaccag cagcotattt aactgtttac tctgggaaaa
97741 ttgttgctgg tttaggtttt agggatcaga tattacaggc aacactgaat ggaataactt
97801 ggcaggagtt atgctttaaa attattgtca aatgattaaa ccttgattta tttattccac
97861 attccagggt ttataaccaa aagattagta ataacatgac ttaacttttg attcgatttc
97921 tgacattaac agatgtttgg aatgtgagtt aaaattatgc tattttcctg atttcttaat
97981 ccaaaaagta tagacagaag ttaatgggat tgagaataac tatgacattt tttagtttat
98041 tcatagtgca tttccttagt gccaagtttt cctacctttt cccattcttc ttttccttct
98101 tctttatatt caacgatgta tccagttact ttggatccac catcttttag tgggggagac
98161 cactccagat ctgcagatga tttagtccaa tctgtcactt tgggaaatgg aggaccagga
98221 ggggctgcaa agagccagta tacgttagKa ttcttgactt ttccaaggca cttttggaaa
98281 ataagattNa aaaaaaagga atggtttcca ggcttaccaa ttggatctct agcagtcgct
98341 gggtctgatg gcagacttgc tggacccacg ccagcagcat tgattgcata tacccggaat
98401 tgatagtcgg aaccttcaat aagaccagtc actttataag aaacacccaa agtcatggct
98461 ttgataggat ctcggttaac tctcttccat ctctttgaag tggtgtcttt catttccagc
98521 cagtaccctg tcacaggaga gcctccatca tattctggct cttcccagtt gacagtcatg
98581 gagttacgag tcacgctgct aactgttggt ttatctggtg ctccagggac agctgtgaaa
98641 aagatcatat tgattataag aaatttaaaa aaaaagtaaa aatggctttg tatgtgaaaa
98701 tgttctccta cttacagaag aggtttcttg ctgtttcagg ttcactgtca agaggttctc
98761 caatgccata tttattctgg gccatgattc ggaatacata ttcatggcct tctagcaatt
98821 tgggaatcgt gtacgtgcac tccttaggtt cactggagac atggacccat gtcttcctgt
98881 tagcttctct tttctcaatt acatagtttg taatcttaga cccaccatca tctttaggtg
98941 gaaaccaaga tagtgtcatt tgatctgctg aaacagattc aaattttatg ggtcccactg
99001 gagggccagg acgacctaaa atggtttaaa gaaggaaccc tttatcactc agatgatttt
99061 aaagccaaat gttatttatg aacagaaRaa caagtaattt taaccaaacc atgcagtctt
99121 tacccaggac attcagtctc atctcctttg atgctgttcc cagattattt acagctgtga
99181 tggtatataa gccagtgtca ctcctgcgag actgcggaat aactaaagtg caagtatcat
99241 ctaccaccag tttgttgaca tgggtgtcat agagaacagg ttctttgtta tcaggcttct
99301 ttggaggagc tttaaaccag gttagtgtcg ggaatggcac acctttaatt ttggccacaa
99361 tgttaacatY ggttccttct tcaacctcca tgaattcttt tagatcaatt gatggtgggc
99421 ctagattatt taaaaaaagt tgtcattagg agcaaaaagc attgagggat agaaagtaga
99481 attcacagtt actataaagt tgtttggatc cactgtaggc agactttgta gctcagccca
99541 atataaagaa tggttctgtc tttaacacta ttacgctagt ttggggagca ggtacacggg
99601 gtcagcatct tttattggat ctacatgtgg ctatgtatct gtgcttgcct cactttattt
99661 ggcatcatta tgcatgtaat gatgtgtatt attatattct catatcctga caaaagggaa
99721 gagaggagat tgtagtagga gagtatcatg ggatactctc tttaatattt acacagaatt
99781 atgaagggat acacaagaag gtgaattccc cttttaagaa atataactgt taataaaagg
99841 aaagcaattt tccattttgc acaaatttag gacagaaata ttcaaagagg gtagtgacta
99901 cagtttcttc acacttagat gagagaataa acaaatacac aacagtagtg atggtaatta
99961 tcagaggaag aattattacc atatatatat atatatatat atatatatat acttcattaa
100021 aatggatata tcttttctca tatagagaaa taaaaacttg attgtgaaga taaatgggtc
100081 tcagcagttg ctaagaaaat gactggttac tttctgtccc ttttgaggag catgcagaga
100141 aattgcttta gcctctgata aataaaacag actgcgagga aaattacagc gaggaaatta
100201 cagacatcat ctcctttgaa cagggaaaag gtaacaggta agggaatgtg aaaattctgt
100261 ggaaattgaa ggaatgagta attaagtaat gtgcccatgt ctacattcaa gccatagtag
100321 cttcctaagg tacagatata gctcttttaa ttgtgaacta ttattgaaca cctaggaagg
100381 cagctgtaag gaggacattc tttgtcagta cattggtact tacatgtctg gtctttgaca
100441 gtcacagggc caacagtggc tgaaggcttg ctgactcctg cagcattgac agctttgact
100501 cggaattcat attccccacc ctctactagg tcttcaacag taaattgcag ttcttccaca
100561 tcacgcttat tgcactgcct ccaggcttct ttctttgtcc cagtagggtc ccatgcaagg
100621 cactcaacaa tatagtgggt aacaggggag cccccatcat tcttcggggg tttccatgac 100681 agatgcactg tgttctttgt gatgaggcca atcttgagtt taataggagg atcaggagga
100741 tctgtaaaaa taattaaagg aagtattaag cgttgtttat aataatatac ttcaattttg
100801 agaagatatt ttaaatttag attttaaagc ttacatatcg gatctctggc agtggtcctc
100861 tgaatggttt ccacaggtgg gccaatacca aaacggtttt ctgctcgcac acggaagaaa
100921 tattgctgtt cagagaggag atcaggcact acaaatgtgg tgcttccaca gtctggattg
100981 actttggtcc aggcttttcc atcaatagtc ctcttctcaa taacatagcc tttgatcctg
101041 tctcctccat cgtcgtctgg catcttccat gaaagtctgc aactacccct tgtgatatca
101101 ctgactttca gatctttggg tgggcctggt acatctgttg gatgtaaatc acaatataag
101161 caacgttcct taaatgctta aaaataattt gtttttattc tgtagcaact ttcaaagtct
101221 ttcttaccca tgactttaac tctgcaattt gcagtctttt gtcctgcttt attcttggct
101281 gtgatgctgt atttgcctgt atgagatcgt ttacactccg gaataataat tactgaggag
101341 ttttcagcag tctccagctg tacaaagaaa atagtagtca tacattgaat gaaatcatag
101401 caatattgaa gtcaaccata ttctgaatta ttttaattat tattttttta cctgtgcatc
101461 ttcgggtatg tcatgaactc catcctatta gaaaaggaga cagtcagttg tagtataaat
101521 actccttata gtgattcagt ggaaaaaaga ggagaatggt tattttccta ccttcattgc
101581 tttctttgcc tttccatcaa attcccaaga tgattttggt gttgggcgtc ccttgatgac
101641 agcaggaatc ctaatctgtg agccagcttt acaaaccaga cagtcctgtg ctccaatgtc
101701 aatgaaaact tctggttcct ctgtaatacc acatacaatt taacaggatt tagctcatat
101761 ttgtcaaaag taattgaaat atcttcagta aattaatcaa tttcataata agatcaaaag
101821 aggaatacat aaactgagta agtactagta ccttgaatat cagtggcagg gatctcccca
101881 gttgtatcac ttggttcaga ttcaccagct tcattgacag ctttcactct gaatctgtac
101941 ttcctgagct ctttcagatt aggcaccaca cattcacagg tagttataag tttgtctggt
102001 tcatttactc ttttccagtc agtagtacct tcttcttgca tttctacaat gtatcctttg
102061 attgggctgc caccatcttt ggctggaggt ttccatccta gtgagatgct tgacttcgtt
102121 ttatctttaa cttctgggca agaaggtggc cccggtggaa ctaataacaa aagaaaaaaa
102181 aaagcttcat cagattttga agctgatgaa gtgctggagt cttttactac acatgatcat
102241 atattatttt aatctattaa aatattaaaa tagaaatgct aaaaggaaat acagttgcct
102301 tctagagaag agaaataaag gaaatggtct attttccttt agcattaatg caaaaacaga
102361 gaaagcaaac aactaactgg gaatagaaag actacttcct atcttgttag atttacattc
102421 tatttataca tgattggtaa aaaactactt tacttttgag atccctttga agaaattcta
102481 caatagcctt tactgaaaac atgatttaaa tttaatttgc cacaatatga aaatttaaaa
102541 aggattccat caatagctag taaacctttg aacacaaact aatcttaatg ccctttaggg
102601 acaggatcag tgtctacaat aatcccataa caatccttta cctttctgaa agtgctttac
102661 aattacagaa attcttttac tgtacttgtc tttttaatct taacttttcc ccctccagtg
102721 gttaatatag atccttgcac aaagcacatg ttcaatacta gtttgcaaat gaatgaataa
102781 ctacctcatt atataggtat atatgctcca catagcactt gagtatttaa aattttcttt
102841 ttaacaaata attgaatttt tcttctatag agaatctaat cagatatgta gcaaatagga
102901 tgaattatta tggccacagt attttttaat ttttgaacga atctgctcac agatctttta
102961 gaagaatttt tctctcttat ttgacctcac agactatgcc tgagaatcct aaatctttgt
103021 tcaataatat tatagaaaag ctaacaacat tctgtttttt gttgaattcc tactggaagg
103081 tctgactgtt tagtgggaga gatgatttcc ccagtatcta atgagataga aatttattta
103141 aagtatcaga agacatagga gtttatagtc caggtcttta ttattgtgat tcctcataaa
103201 atatttggtt taatccaaga gtttacaaac tgtatgattt tatgtatgtg tgtatgtata
103261 tatctgtgca tgtatgtata cacacataaa tatatacttc cgatagtcta tgtgaacatg
103321 aacctttttt attttaataa ataaaataat ttggcataga aaaatataag aaatttaagg
103381 cagaattatc catttagtga ctttgtgctt tagaaattag tccccagaaa cggaagcata
103441 cttacatatg ggatctcctg caacctctgg atctgatggt tcactgggag gaccaattcc
103501 tgcagcattt attgccatgg ctctgaactg gtatttaacg ccttcaagca aacgaggaac
103561 attaaattcc acgcctttca atcccacttt ggttattggt gctcggctaa cacgtgacca
103621 ataaggactt ccctcttctc ttttctccag ccagtagcca gtaattggag agccaccatt
103681 gtccaaagga ggagtccagc tcactagcat tgatcctttg gtgcgtgcca aaacttttgg
103741 ttttcctgga ggtccaggaa ggcgataagg atcttgaatg actactttat ctgatttgca
103801 ctcatcactg attccatatt tattcactgc cctgactcgg aactcatact gaccattggg
103861 gataagtttc cagatctaga aattagaaaa acagaaattt attgaagaga atatacttat
103921 gttggttttc actcatttaa aaattcaact tattttggga gggatgactg taagaagaaa
103981 taaatatata gactcaaaac atgggaagac agagaaaagg agaaagcttt ttttaaaata
104041 aaaatatgta gatgtattgt acctgatagt ggatctcaat taaaagagta tctgtgtatc
104101 aaacgctatg- tactaaggag ccttatgtgt gtggggctaa acactacaat aacaaaataa
104161 cagcttatcg tgtgtggttt tgagtttaat tatcaaattc caaggttata ttaaaatggc
104221 atcaaaccag agtcatgtac ttttaatgtg tataagaaaa tattcagaag agtttacccc
104281 atatcttttc tctacagcag tgttggtgac ttcctcccat tctgctttct tcctacttgc
104341 atcacgtttg tcaataacat aatgggtgat ttcactgcca ccattatcaa gaggggcatc
104401 ccatgtcaag tagcaagatt cagctttaat gtcagtaaca gcaagatttc ttggtgggga
104461 tgggcggtct ggaaaggaat caacagagaa tattgagaag gcaattctta aacagacact
104521 ggatgccttt ttgatgtaag aaagcaagtc acttaccRta tacttcaacg tgaacatttc
104581 ggaacactga gccaaggcga ttggaagcag taactgtgta agtgcctttg tcctcccgga 104641 ccgctttggg aatgctaagc tcagtttttg cctcacttcg ggatacctct tccttggtta
104701 tctgaagtgc atcagtgggt ttttcaatta cagtttcatt tttggaccat tcaatcttag
104761 gcattggaag gcctgtcaca tctgcaggga tgtggacagg ctctcctgcc ttaactttga
104821 tagtgtctcc tcgaacactc agacgcaact taatagttgg aggcactgca aagagaagag
104881 aaagaaaaaM agtaacaaag tcataaggat gtttttttca acttatggga taaaggcaca
104941 ctgtaaaatg cattaaaatt attattatat tcagattccg caccttcatc atcttgtatg
105001 actacattaa gaggtaggga tggttcactt tcaccaattt cattgacagc tttgacacgg
105061 aactcataca tttggtgttc atcaagattt tcaaccagaa aagatgtggt tggg'cagagt
105121 cgcttgttaa ctctttcaaa gtcaggtttg tcatgacgcc gtttttcaat gatatatcct
105181 tggatgggac tgccaccatt actgcggggc tctttccagt caagtgtgat agtggacttt
105241 gtcctttcag tgtatgtgag cctctctgga gatgttggag gacctttagc cagaggcaag
105301 tgaaaatgat tagcatgaga taaatattca tgtaagaaat aatatttcac ttcaacttaa
105361 ttatgtaaaa aatagccttc tgtttggcat aaccttacat ccttatttaa aatcattcct
105421 aaaacttgtc agttgccaaa attatagaca ttcatgtaga cagaattttt cttagcaaac
105481 ttttcatcaa gattatcagt gaaaaataaa tcaagggcac gtatgctttt ttaatttaac
105541 attttagatt gtttaccttt gtaactgggg atgcttctaa ttctactgaa ctaggaaagt
105601 aagtctgtga agctttgctt tgaatgacca gctgagagca aagtgttttt tcttttatta
105661 acagatagtg gatatgtcta tgaacatact ttgagtcatg ttgcctcaca tgtgggttca
105721 aaatatggtg gaaagaggat gtatgtaatg ctgctgaagt tttctttcct gaaggaccaa
105781 actaagagtt aagctttcag tggtaaaact agacccaatc catacttgtt gactgttatt
105841 gcaaccagaa ggagtggaag ctcagctctg ttctggtgcc tctggcacag cctgtggtat
105901 attggacgtt catagatgag ctgcagcagt gatataggat gtattttttt ttttcagggg
105961 gtgatggtga aggaataagt tcttctgtct cttgaggaat gggtcttcct caaagccttt
106021 gctttcttag atgggatgtc agtgtgcatt actgtgtgtg caattttagt gtttcgtatt
106081 gatttctggg agattccctc tggttggttt tctattcttt ggaggctgtt tctggttttg
106141 ggcctctcta tccttccagt atggggactt tttgaatata gaggaagact aaggcattta
106201 ttctcaggaa ttgtctaatc ttttagaaga acattgaatt ctctttaact actacgctag
106261 taagttttaa aaacccgact tccaatgtgt tctactggag agatataata tgtctaaact
106321 acttctaggt tattattaag cccctgtaga tccaagtttt tcagtacaag aaaccatgca
106381 tgattaatat acttacagcc acaaattaca ttgaaattat aggaaatatg atatctttaa
106441 tgttgctaat agttttttga tttacttttc ttattactct ttagctatag attttgagac
106501 tgttgggagt ttgaagccat aaagctcagt aaataatata actagaagca aaacattact
106561 ctataaacac aatgaaacct tctcttttac ctagtggatc aactgcaaga attggtccta
106621 tttcaacagg agggccacag ccaaacttgt tcttggcaat aacacggaac ttatattctt
106681 gtccctcaag cagacctgtg atgtcacatt tagatctctc agtctctatt ggttgtctcc
106741 aagtatgcat cttggcatct tttctttcaa tgataaagcc tgttatttca cttcctccat
106801 catcttctgg atcagaccag ttaagtagac acatttttct gtttgttaca acaggtttta
106861 agtcaagaac tggaccaggg acatctgaaa acaaaacaaa gccaaaaatc aatgtaataa
106921 gatagtatca cttgggaaaa tcctgtgaaa atcagtgcaa cattccttac caaaaacttc
106981 taccctggct gctgcaaatt tggaaccaca gctatttgta gctgtaatca catatctgcc
107041 atggtctttt cgcagtgcat ccttgataat tagttcagat ttggttccaa cgttgtctat
107101 tacaacacgg tctttatcca gctccccttc ttctwtggtc catacttttg taggtacagg
107161 gcgaccagtc accacagctg gaattctaag agtcttccca gccattattt gtattccacc
107221 cttgacagaa acatccagtt ccacagctgg aggctctgtt gaaagagaac agtcatgcat
107281 tagcccatga aagattaaaa aatagtccca tatagagaaa atatccataa ttttattcca
107341 ataacgttaa gtaccttgtg gttcagccac agtaacaggt tgtgtttctc caggcggtcc
107401 ttcccctgcg gcattgacag cactcacccg aagtttgtaa tcagcaccct ctcggatttc
107461 tttgacggtg tactgacgga ccttgatcat cttctctgtg cagcgtgacc attcatttgt
107521 gccaaccaac tgcttRtcaa caaaatagcc aacaatttcc ccaccaccat tgaaagctgg
107581 gggttcccat tctagttcaa tagttgtaga gcttgtgtca gtgactcttg ggataggtgg
107641 cccaggagga gctagaatga atgaagatag acaaatcaaa ctccctgatg gttttaaaat
107701 gtacaatagc ttgttctact ccaccttctt cttaaggaac tacttacaga ttggatcatg
107761 tgctgttttg ggatctgaag gtggactgaa ctttccaggt ccagctgcat tttcagcaca
107821 tactctgaag acataggtga gtccttctaa taagccatct acattggctt tcaaggcatt
107881 cagaaggctt ttgttgacac gagaccaatg tgtactgtta acttcacgtt tttcaagcca
107941 gtaacccaaa atggggcttc cattatcttt tggttcattc catttcacta gcatactgtt
108001 gctggtaaca tcttccacaa tgggcttatc tggtgcatca ggtggtcctg ataaaaaaat
108061 aacatttgaa gtaaatttcc cagtatgaca taaaaatgca attttaaaag ctgtagtaat
108121 tatataccaa agtacatggt aaaaaggcaa actttagatg ccaaaggaaa cttaccaaat
108181 ggatctttag ccacaagtgg ctttgaaaca catggtggac ctggcccaaa tctgttttca
108241 gctcttacac ggaagaggta ctcttttcct tcaatcagtt ttgttactga atatttgcaa
108301 tgtctaagtg ttgaagtgac aacaatccat tctgagtcgg gttttgtctt gtctttcttt
108361 tccaaagtgt agtttatgat ttcactgcca ccatcatcaa ggggtggttc ccatttacaa
108421 aggactgagg tctttctgat atcttcaaat acaaagttga ttggtggtcc cggtgtatct
108481 aatatttcag aagagaacag taatcaaaaa tttgtgtggg aagggttgct atggaaaata
108541 taaatgtgag catgctcctc tgacatgatt tgtggttttc ccacatctat ctttgtattc 108601 cattagctaa taaataacaa gatcaggtga tttcttttac atggccagca gataaaatat
108661 gattgaggat aacttgctga agctatgtcc catttctttt aactctctcc caaacttttt
108721 atttttatgt tttactaaaa cctttttcca ttggaaagtg tagttttaat gactcaccta
108781 acacactgac agtacaagga gcttttgcaa taccgtggtc attttcaact ttgatcatat
108841 acagaccatg gtcaggtcgg agagaatctc ggacgcgtag ctgagattct ccctttttgc
108901 tgttgtcaat actcaaacgc tgtgtcagag gcaggacaaa tggttcttct tcttgaactt
108961 cacccttcct tctcctaacc aagggtgctg cacgcttctt aatttcctct ggtacaataa
109021 cattttcatc ctttatccat gtaatagttg ggtaaggtga tccagaaata cttgcatcaa
109081 gtgctatttc atcacctcgt ttcacttcta ggcttgttct cagaatgact ttgggggcat
109141 ctatagtgat cataaccaat aaRtgttttc aattctgatg aaaataactg acaaaacatt
109201 attttcattt atttcaaaat gggaaagtgg caactccatg aaacaaaact taccaatggg
109261 atctacagct ttggttggag gagtagcccg agaaggttca ctaataccYg cggcgttctc
109321 tgctcgcaca cggaattggt actctttccc ctcttcaagt ccttttgctg tataggtcag
109381 gattggtacc aggtgttcat tgcatctctt ccacctttct tcacccttag caatcttctc
109441 tatgatgtag cccattatct tgctccctcc atcatacaaa ggtggcttcc atgtaatagt
109501 cattgcctcc gctgtaggat tatgaacctc tacatctaca ggtggatcag ggggttctga
109561 agaacaagaa aaaaatgtta gtatcaggaa aaccaccttc ttaaaacaaa actatggttt
109621 attagttctt agccatagtg catccatgtc caaacttacg ctttggatct tgagcaatga
109681 ctggattttt cagttcaata tattctcctc caccaacctt gttgactgct ttaacacgga
109741 agaagtattc accatttggt atgagatcct tcacagtcca tgacagtttg ttctcaccag
109801 acatgactgg tatatatgtt ctcctcccag cttctctgcg ctccaggaca taatgaagaa
109861 ttggggttcc accatcaaat tctggaggtt cccaagttaa cttacaagaa ctcttggtaa
109921 tgtcacttgc tttaatatct ttgcatggtc caggtaggcc tattacaaaa atggataatt
1099-81 attctagtaa tcctgaaaaa tcagcatttt aataatgcac ttgtctctaa gtggggctat
110041 ctgtattttg ggtaggataa tctttgatgt gctggagtg't cattcacact gcagagcatt
110101 tagcatcctt ggctcccaac tactaaatgt cagtggtatc tacctcaagt ctgtgtgcca
110161 agcaaaaatg gccccacaaa tttccagaca ttatttgcag ggctgggggg aatgcagaaa
110221 atgttactat ccctggttaa gaacactatg ggaaaatgtg aagctgtgca attaaaaaat
110281 tgtggtcatt tcaatgctct ttgggtatgc taatatctgt tatttaaatt agtatttcac
110341 atgcaattaa ctaaaactag tataaattag tctaataagc gtttagcgat ttcatctctt
110401 tagaattgtt tYacttctcc tatggaaaga cataatcaga agtgacattt agtttacaag
110461 tggccagttc atgaagccaa ttatattatt aatagcactg caaagttaac taatttcctc
110521 tacatgtgaa gaatgtctgg tttttcttca aagaatgatt acctatgact ttgacattga
110581 ttgaggctgt tgctgagccg agcttattct ccagtgtaat ggtataaatt ccggcatctg
110641 cacggacact cttgggaact tcaaggtgtg cagagatgtg atcattcttc attgttaatc
110701 tatcacttgc tttaacttct ttgccatctt tatgccaact aacagttgga actgggacag
110761 ctctgaaggg aacaggaatg cttaactttt caccttcaat aacaataatg tcatgagtct
110821 ccaggtcaat tgttggcttg cctgtaagat atcattcaaa agagcaaaaa acagagtatg
110881 tcaaaatgca atgcatgaat aaagggtatt ttattaacta taaaagacct tgtgctctta
110941 cagtctgggt ctttggcaac cacattttca gagatttcag atggctcgct gacaccaata
111001 gcattgactg ctctcactct caggacatat tctttgtcag gaacaacacc ttcttcaacc
111061 ttgaatttca agtcctttat tgggcgagaa ttaactctca tccatttctc agtgcctact
111121 ggacacattt caacatggta tcctatgata ggacttccac catttttctc tggaggcttc
111181 caagcaatgg caatgtgttt tctcccagca tcagtcacat gtaggtcaag gggtggtgag
111241 ggaggacctg agaaaagagt gaaatattca tatccacagt ctcatcaagt tctagacaat
111301 atcttgtgta taatcagcac tacttacttg ttggatcttc aatggatagg atttctgtgg
111361 gttcacttgg gtggccaact ccagcttcat tttcagcccg aacctgaaac tggacctctg
111421 ttccttcgat gacatcagtt actctgaagt tacagtcagg tcctgcagtc tttccagctt
111481 tcacccaacg ggtacctaac ctttctttct tctcaatgac atatctattg aaacaacaaa
111541 gcatttcatt agcattaatg aaaaaacaca gttaagaatt tgcagccaag tgtaagttat
111601 tctttatgaa ttaattcaaa ttctacctct gtattggtct tccaccatca tttttaggtg
111661 gctcccactt taggtcaaca tgtcgttttg tcacatcaac cactgccagg gcgtagggtg
111721 gtccaggagt ggctgaaaat aaaataaaca atgattagga aaaccagagg aaaggtgata
111781 atctcctgtc acaaaaatta agtgcgagag cacttattgt cacaaagatt aagtgcaaga
111841 gcatactaaa ggtgtctttg gccagcacag agtcctcaat ttcggtgtag tcgctttgtc
111901 ctatagcatt ttctgcagca actcggaaca catataaaga gccctcagtc agtggggtga
111961 ctgtgcactt ggtgtccttg acagtggtat ccactgtttg ccagcctttt cgcctgacgt
112021 ctctctttto cacaatgtag ttggtgatgg gggaccctcc atctttctca ggaactgtcc
112081 attttaggtc tgctgtattt tttgtgatat taataacttc aagccagcgt ggtggtgatg
112141 gaggatctga aaaagaagga aggaaaacaa attcattttt ttttttatta cccaatagtc
112201 agtctgaaag tgcaggcagt cattaagaag taatgtagcc aggaggaaat aatagtaatg
112261 aatttttcat attttctcat atcgtagctc acaggcagat attagaagaa tttatatatc
112321 ccagacatca agagtgactt acatagcttc tcccggcaaa gcacagggtc actgggttca
112381 ctgggttcac tttccccagc cttattcaca gctctaactc ggaatgagta ttcctgtcct
112441 tccatgagcc ctgggagcaa gtgctgagtg gtgggaaccc cttccgccac tctgacccac
112501 tcatctgttc cagtcttcag catttcaatg acataagact caatctttgc acctccatcR 112561 tgttctggtt ttgtccaatt caaccttact gatgttttgc ctacatcttt tacagttggt
112621 tttccagggg gccatggagg atctgcaagc caatgaaatc attgtttagg tttgtcaaaa
112681 aggagttcat atgaacttcg aaataaccac aaaaattata taaataatac ctatgggatc
112741 ctttattaag attggttcag ttgatttgct tggttttcca ggtccagcaa gattttcagc
112801 caacacacgg aatctgtatt ttttcttatt agtgagacct ttcactctga attaggaaca
112861 aagtgcatgt gtatcatcat ggtaagaagc agaagaagcc tatgtttatt ttacacagaa
112921 gagactaatt tatttaaatt gtgaaaaggt gatttggaaa tttaactgaa aagcacttca
112981 atataaatta aaaattgctt tttaactgcc acacacttta gactgggcca aacataccaa
113041 tctaattata atatctatga cagtggtcat tatcataata tactggggga tcaaacactt
113101 tgaaacttcc tgaaggttaa tattttagag taaactaaga aatgtgccct tgaatggaat
113161 ttatataatt tttcaaatta tgaggtaata gaaaatatga gtaaaagtag tttaaaattt
113221 ttttagtaat atataatcat ctgtaatccc aaggaatact aaagagtaaa catttgaata
113281 tactgctgtc ttaacatctt gatggggatt ctgagcatac ctgtatgttg tgtcctttac
113341 tggcatctta ttgcatctaa cccatttatc tgtatcagga tccagtcttt caacccagta
113401 tcctgtgatt gggctgccac catcatcatc tggctcacac catgtgagag tcactgcgtc
113461 tttagtgata tcagaaggtt ctaggcgagt tggaggacca ggtggatcta tagacaggat
113521 aatggtgtaa aatgttatat gtcctgtata taaatatgac accaaaatgc aagtttgaca
113581 taagcatcct ttttttttta tcagctgatt gagaaactta ccaaactgat atttggctgt
113641 tattggagag gcctgaactg gttcaccaac accatacatg ttttctgcag caactctgaa
113701 gatgtactct ttattgggga ttaatttggt ggccttgaag tttgtatcct tgacggtgga
113761 tgagagcttg tgccacactt cactatcagt tgctcgtctc tccacaacat agtttgtgat
113821 cttagatcca ccatcatcgc gtggtgggtt ccatgttaga agacatgact cattggttac
113881 atctgtgatg tcaaaggcag ctggtggtcc gggtttatct gtgtatggca ttacagatgc
113941 agaaaaaaaa attgttcacN atttttttta tttttttctt tcaagaaagt aactgcaaag
114001 agtttatccc cttttaaaat gagaaccttc cttacctaag acgttcactt ccaccacagc
114061 agtggcccgg ccacacacat tcacagcctc aatgatatat gtgccagtgt cacttctctt
114121 actatcaaca atagtcactg tggatttctt agggacattt tcaatggtaa ttcttttgtc
114181 ctgcttcaga atcatatcag cctttgtcca agttatttta ggttcaggtt ttccggttac
114241 gtggcagga agttcaatct tggtcccagc ttttacagtg agaccagcaa ggagcttcac
114301 atcgaggaag atttctgggg cctctgaatt ggaaaagatt atttatgatg ttatcaagtt
114361 caagcagatt taaggtcaga ggcctatttg ataagacaaa aatcaaaaat agataccttg
114421 tgtgtccaca gcctggattt cctctgtggg tctgcttggt ttgctagcac cctgcctgtt
114481 taaggccttc acgcggtagg cataccattt gccttcctca agacctgtca cttccattct
114541 gtcagaaaac agaataggat gttttactgt gatgtcgata atcgtttcat tgtgtagtac
114601 tcataatctt tcaaaaattc aaacccctgg tataatacta gtctttaaat taaacctgga
114661 ttacttcaac actataactg aatacatttt catttcaata ctgtcatgaa aaccctgagc
114721 aggcagtaca ttcagcagca cctttaaaaa tcagacagat acaccggcag cataggattc
114781 tcaatgaaaa aagacaaaca tttaaatttt ccccaacaaa gcccatttta gtgactagga
114841 gtacacattt actctcatgc caaattaaaa acctactttg tttctgcaac aggttctcca
114901 caggcaaccc atttatcaga accacgtgga catctttcaa ctatatatcc tttgatgcgt
114961 gaaccaccat catttttagg tggatcccat gttaagaaga tgctattggc tgttcgatct
115021 ctccatttaa cattctctgg tgggtcaggt accgctacaa catttggaag agagagtcag
115081 tcttacacag gccacctatg cagttctctt cacattgcca acccctgcct tgccccataa
115141 cttcaactct aggtctaaaa gcaaaaacag gcaacaagat taggtaagaa atcatcaaga
115201 atRtactcac ttgcaggagt tgacatattt acaggttcat caacatatgc aggttctcca
115261 gggccacatt tgttacKagc acaaacttta aataagtact cttttccttg aacaagatca
115321 ggaactgtga attctagatc agtcacaaag tccataacct gggacaaaga aatacagtta
115381 atcagttatt tccaaaaaac cctttcgcca acccaaaaga tttttaccat gaccagggat
115441 acaaactgca gttgttttga atgctaggga ttattcttac attaatttca tattagggaa
115501 aattgcaaag taggatacat gataatattt gactcaatat aagaaaaact gcttcatagt
115561 ttgtacacat gaaaaaagat ggcaaatgag gtgttgattc taccagtttg cttatcagaa
115621 atatgatttt aattcagggt aaagaatagt aactaatata aacacaagga aataatggct
115681 gattaggtca actaaggtat tctagtggaa taagaagtgt gaccaccagg gaaataaata
115741 tttactttcc acttctagct taatgggtac atattgggta cttagcaggt accaagtcag 115801 tagggaattt ttttttttat tacaaatttg gtaaggctca caagacaaga caattacaat
115861 tatacaatcc ttggtYgagc tacctcagga agcattagca ccgatgcatt cttaaaaagt 115921 ttttgtacat tttcagtgag gtaaaggtaa gaagttgtag cacataagac ttttgtatgg 115981 catcccaacc ttctgggatt tcccctactc tcatttttgg cattgatgca gtgctgctca 116041 aaactcactt tagtccatgt tttccggctg acttctcgtt tttcaacaac gtatccagtt 116101 aacggacttc ctccatcatc atcaggtggt tcccaagtca aatgtactga gtccttggtt 116161 atatcaccaa atttcaattc tttgggtgca cttgggcgag ctgaaaaaaa atgcactcac 116221 gagtcactgt taatagtctg aactaatatt tgttaatact gccaaatcac cttagatgat 116281 aaatgttttg ttccttacca attacattga catcaatttc cccagaaatt gttttcacac 116341 ggttttctaa tttcagtgta taaatgccct tgtctgaacg ttcacttgga gaaatgacaa 116401 gttcggcata ggcagacaag gtcttcattt tcacccggtc ccctgtttct agtactttat 116461 ctccaaaaca ccaggttgca gttggccttg gatagcctgt acttggaacc aggatcgtga 116521 taggatttgg gacaataact tccagaccat ctttaaatgc acttaaatcc attgttggtt
116581 caactacaaa gaagaaaagt taatgagttt gcagtgccat atttaagtcc ctgttgcccc
116641 aaagtagcta -tgtgctattc cccgatctaa aaataaaata tctatttacc aaatgcatcg
116701 tcagcggtga gaggaccaag aatttcagat ggatctgaaa caccagcttt attttctgca
116761 gatactctat ataaatattt atttcctttt tccaatccgg taacctacga ttatgaatta
116821 atatttgtaa aaaacacata cagttatgtc catgatccac attgaatatt cttttttata
116881 tgcaaatgac ctaccttgta agtcagttca gggacaagtt tcatattgca acgaatccaa
116941 ttatcttttc cttcttcgca tcgctcaatt atatagccta atattgggct tcctccatct
117001 ttcagaggag gttcccattt gagctgaact gatgactgag tctgatctga ggaagttagg
117061 tttacaggag gacttggtct ctctggaata aaagaaggag ttggagcata ccatttacgt
117121 tagtgacaat ttatgaaaac agtgttgtaa tcNNNNatat catcctcatt aacaggttta
117181 tcaaattcac taagtaaagt aggcacagtc tgggtttcta aaactttatc caaattttta
117241 tgatattcta cctttttatt aactttcatt taacttgatt tattttaatt gataggccta
117301 atatctggat ttcatgcagt aattatttcc cttttttgat gggcttacca attggatcag
117361 caactttgac gaagggtgtg gctgcacttg gttttccaac tccaattcga ttttgggctc
117421 tcactcggaa actgtactcc tgtccttcta ccacatcagt aacagttgca gacaggtctt
117481 cagctctcac agtcatggca gtatcccacc tgatagaagt cttgtctctt aattcaatga
117541 cgtagtttgt gatctcagca cctccatcat actctggtgg ttcccatgtc agtgagacac
117601 caaatcgatt cacatcagtg atggttacat tcaaaggagg gcctggaaca tctggatttc
117661 accacagaag aagaaaatat gagtttgggg taacgatgat gaaggcaaag actgcaattt
117721 acttaaaagc taacttgaat ttacttaaaa cttcttacca tatttactcc ttgcttctac
117781 aggattgtca gtttctactg gctcaccagt gccaactctg tttcttgcac tcacacggaa
117841 taggtactca actcctcctt tctgtagacc agtgacagta aactcacaac tctctgcacg
117901 gtctgtggcc agaacccagg tctttctctt aatgtcacgt ctttcaacaa cgtaacctat
117961 gattttgctt ccaccatcag ttaaaggttc ttcccaagca aggctcactt caccatcaaa
118021 tgtttctgtc acttctaagt tacgtactgg cccaggaaca tctgaaattc acatatagag
118081 gaattttttt agtgtgctgt cttgctttta aattgtgctt tgccaattaa gtctgagaga
118141 cccaagggct taccaataac ttttaaattg atgaatcctt ctgcttttcc atgtttgttc
118201 tgaagcacaa ttttatacct ccctttgtct cctttcttgg cttctaaaat tctgaaagaa
118261 gtttgttcag ccgtagtatc aatggttttt gtagataaag gttcattttc tttaaaccat
118321 tcagcttctg ctttggggta ggcatcatat ggcaccacca ttgtcagagg cttgccaaca
118381 tcaaccacaa ggtcttggtc agctgtcttg atttttggtg cagctagtga gaaagataac
118441 atgtgaacgc tttcgactat taaacgtagc caagctacat catgttatta tgcatttatt
118501 agaaaatatt ggttacataa ccttggaagt aagctacagt aactttatag cagagaaaac
118561 taagtggctt agagttctca tagctttttg ttgttgttgt gcgtgcaaat tagattggag
118621 agtagaggat tgagaatggc ataaaatcat aagacatgca ttccttgcca aaaatagtaa
118681 cataggactc agtgtagtgc aaaacatgtt ttgttttgtt ttttgataga gtaggcactg
118741 catacttagt tcaataaatc agagaaactt ttttcttatg aatgagaggt acactaatgg
118801 ctttttcata aggacagtgt ataaggatgt cctgggtcag gatgagaaca tacctaaaag
118861 gcacacaggg gtttgtaaag taggagagta taagacYgtc tttagatttg ccaagactct
118921 cagggtgata gcttagttgt agcctaagtt cataaatagt catcatgtac tagcaactgt
118981 ccaaggctct ggcatgtaaa gattattgct attaatttcc tcttactcaa gacacttact
119041 ttgtagtgag caaaattaga gttgttaaca tgtgggtagg gcttgctaga aatgaaagac
119101 cctcacaagg attacctcat taaataactg tgaaatctga aatctaaact ctgtgatatt
119161 ggaaggatat tttaaaataa agggactgac ctgccagttc aagcttagct ctggcttctt
119221 tgtctttggc aataaatctg tattcaccct ggtcacgggg cttaatatca caaatctgta
119281 gtcgatgtat ctttccttca ctcatcatct ggtgtttatc accctggaca acaaccatgt
119341 tatttcttag ccattggact tccaccttat ctttattgag ttctgctaaa aagacaacat
119401 cagcaccagg ggcttcaaga atatcttgtg gaggcctgat gatctcaaca ggaatttctg
119461 gaaagaaaat gtgaaataaa tacaaatatg tttacatttt gattaacaat tttaaaaaat
119521 tggtaacatt agaattgttt tttcacttaa tatgtacctt ccacaaaaag tctagcacga
119581 gacttcctgt cttctacccc gcaagcatat tcacactcat catccagcct gcaatctttt
119641 ataatgagtc tgtgtatact tccatctttt tcaaatttgt atctaaagga gacattggat
119701 tatcagttag cttgcaatga tggccactaa attgtcaaaa gtgtatatat agctacagaa
119761 atagagaata aaaagatctt gcttactttt tgccttcttt gatttctctc ccatttctgt
119821 accattttac attggctttc tctctggaga gctggcagga gaagacagcg tcatcgaact
119881 cagtgactgt ctgatcttcc aagtgttcca caaattctgt cggggcttct atgatgagaa
119941 gctcagcaac •agatttatct tgtccagcag tgacaatgta ttcaccttca tctgggaagc
120001 cacagtcttt aatggttaac atgtgcttgt acttatcaac tctgtatgag acacggttgt
120061 caaaaggcac ttcttcacca tttttggtcc acttcagtgt tacattgaga cgattcacct
120121 tgcaccagaa tgtgacagat ttcttctcca ttgtttcaat atctttaaga ggctcaacaa
120181 tcctaaggtc ttcctctgtt gtaaaggaga aaaatatgtt gattttaatt atttttataa
120241 cagaaactga tgaaaaaatc tctagtggta tataaattac ttaccttcta ctattagatt
120301 ggctgcactt ttaacatttt cacctctgtg attggtcaaa gacacattat agttggcttg
120361 gtcatctaag tgtgcatctc taattctgag ggtgtagact aggtcttttt ggagaatgat
120421 gtattttgaa ctatcaaata tRgcttcttc atttctgaac catttggctt tggcaccttc 120481 agtattgact tcacagttga agacaacttc ctgttgttcc ttcacccggg tgtccttaag 120541 aggaactacg atcctgagtt tttctgaaag caaccgacaa gactttatag tatgaataat 120601 gatcaaagtg gtaaatacaa atacaaaaca aacaaacaaa caaaaaaccc taaaagcaag 120661 aacaaactta ccaatgactg tcaagtgagc tgctgctctg gcggccccta ccatgacaac 120721 gtaagtgccc atatcttgta atgtggcatc tttcacaact aggaccctct ttttaccatc 120781 agcaataacg tcatatttat ctccagattc aagtgtctta tcatccctct tccactggac 120841 tggaaagctt tcttttgata tagagcagac aaattcagcc ttttctcctt caagtatttc 120901 aaggttttga ggctttgaga taaattcagc agccagttct gggaagaaaa agttacaaga 120961 tattagaaac atatgtcttt gtactttaaa tctagcaagt gtgaattgtt agaaataaaa 121021 gattattcac tgtagttttc ataccatgta ctttgacttt gccatcggtt cgagagcttg 121081 ggagtcgaca gttgtagttg ccagcatcct caaggtgagc gttctgaatg accaggattc 121141 tcttccgtcc ttcagtcagt atttcatatc ttcctgtttc aatgatctcc tcatcccctt 121201 tataccaaat cacttctgcg ccaggtttgg agacttcaca aaccagtttt atagtgtctg 121261 tttcactaac ttcaatgttg gcaagatttt tggtaaagac agcttcttct tctgcaagca 121321 ttgaaaaaac aaaaaccaca ttgagttaag ctggaaatta ttattttttc ccaacacata 121381 tacttcacaa agaatgactt tactcttacc cagaactgtc agcatgccag aagttctcgc 121441 tgtccttact tcacaggaat attcagcttc atcatccagt agacatttgt tgatgacaag 121501 aatgcgcact gctcccttgg atatgatgtc atattttttg ccctttttaa tttcagcacc 121561 atctttaaac cacttaacct atatttaaga taaatagatt atcagttttc ttgttgttcc 121621 ttcacacagg tgtccttaag aaaaactatg atcctgagtt tttctgaaag caaccaacaa 121681 gacttcatag tgtgaagaag aaccaaggtg gcagacacaa atataaacaa accccaaaag 121741 caagaacaat cttaccaatt actgttaagt tagctgctgt gatactatta tcagttatta 121801 aaaacaatgg gatcatataa gtttagagct atagtgagcc ttagacatta tgcagactaa 121861 cttcttggat gtgtaaacta aggctcagac ttgaagaaac ttgccaatgg tagttaatag 121921 caacaggagg tacttagttg aaagaaatat aattgctg'at atttgacttt ctgatctaag 121981 aaaacagcac tttcatatgg ttcaaactaa tatctaaatc tcctgattgt gagtccagtg 122041 tttttcaagt aatagtaaat taataatact aaaaagagag caattaaagt caagaataca 122101 ttttatacta caattatctt taaaagtaaa gtggtgacca gagaagttgt gactaacttt 122161 tttttttcca ttttggctag accaaaagta aatataaagt tatttgacag tacaagatga 122221 caggtataca gtcacagacc ttagcatttt ctcgggaaag ttcacactca aatcgagcca 122281 tttctttttc tctaacttga aggtcggtga gtggtcttaa gaattccaca tgaggagctg 122341 taagagaatg tcatcagaat tcaaaatgag gtttctggaa atttactctg acattaccat 122401 agtatacatt aagaaaaagt gactcttttt tagggatttt ccatcttaat agactacaac 122461 tgactttgaa atcacttgaa gctgcccatc aggtatgatg ctttgctttt tagatatctc 122521 tttagKttac ataaaattat gtggcattct atatgttcaa ctagtgtgca tgtgtgtgtg 122581 tacagtatat atactaggga gccatctcag ttcttttgaa taatagctgg aggaaaaatc 122641 ttcatttgca ctgcaccaga cttgaaagat aatgtcaaag gaaacttcag acaccagtgg 122701 caaagaaaca ttgtcttatt taaggcactt agcacaatct ttgagaaatt agcagaattc 122761 aaagatagaa cacgtgaagc actatggcca gccttgtgta tggctaagag gcatgaggct 122821 gtaattgaaa catacattta gctttttaaa aaaatcagct ttgaagacgt cacatataag 122881 tcaggcataa catctattag tgcaataaag caatgtgttt tgtatgggag ggtgattagg 122941 attttacatc tgttatttca ttcatctgta gaattaattc agctggtgta taggattagg 123001 gagctgcccc cttgcccttc cttctttcag agctccctgt gagtccttcc caagatgacc 123061 attcttgagc aataagattc tgagacaaat gctgtctaat aggtttgaaa atatgctcct 123121 tgcagctgag ccttcttgga gtgaaggtag atgtcaaggt gaagaaggag acccaagaag 123181 ctactttgac ataagcttga gcagagcaac cacaaagtgg taagaaaaac atggtacaag 123241 atgcatgaag cacagagtca ctaggttgta gctgttgaca gatgggaagc aaccaaacca 123301 aacaaacaaa tattgcacaa aaaatttgca gaaaaatgtc atcactggac aaaggaatga 123361 atgaaacaaa taagctagta tgtggcattt tgaaaaaaga cattacattt ttcaccagag 123421 gaagaagtaa tgttaaaaac cattagagtt ctcttcattc aaagagtcca ggactcaaaa 123481 gatccagggc ctcactccat cttcttatcc attcttattc tcagagattg aatcatgtga 123541 atgtgctttg tcatattaca gcagatctct agtatgctYc tattcagact gaaatttcat 123601 gctcattttc tagttgttcc ccacacctga aaattcattt tccttccaca tggaaatcac 123661 ttttactttg tttttaaaat taggactccc tacttatctt taaaattcag tttctcctca 123721 tccatatcag gatatccata cagtaggctg accccatgta gacaaagagc acatctactc 123781 actactcagt gagagaaaag aagcataggg aaaagtgatt gctccatcat taatcagacc 123841 agacatagaa taagactttt cttttctttt tttctttaaa cacataaagg atcaggttag 123901 gtcagtgtoa atactagaag ttgccaagaa atgcagttag gccaagaagg gcatgcaaaa 123961 agtgttgtgt tgttttgttg ttgtagttat taaagaattg caaatgaaaa gtcctttgta 124021 agaagaatac ttacgcacga cattcaggtt acaggaagtc ttaaaatcct tagcatcaca 124081 agtgtatgtt ttaatatcct ctggggtaca gtcatgtata acaagttttc tgaccctgcc 124141 atcagcaaca atttcatact tcttgctttt gaggatttct gtcccatttt tgaaccattt 124201 caccttagca ttttctctgg agacttcaca ctccagcact gcagtggctc cctcttcgac 124261 cgtctggtcc tcaagaggct tagtgaattc aactgggggt tctgaaagaa tcatactcat 124321 tagccaaggt actcctttcc ttctccaaga gttttggtac cttctcaact ttcccctgcc 124381 atctccaggg ctttgttctg tcctaagttt tattttcaag ttttgattac actcagacat 2004/023933
124441 tttcctctaa aaatgatatt ttatcattat ttgtacagac aagaggaaaa tcatttttaa
124501 aaactaggaa acattaagga aataacagcc aactcttggt taataaagtg atggatacct
124561 agcttgataa taattcttcc tccagctggc ttttgaaaat ctaggatgtg caataaaaac
124621 aatagagatg gaaggaggaa gtaaaaccta tgctagtcaa ttttggctct ttaaaaagat
124681 agtctgtgaa ttattttgtt atgctaaaaa gtaaaatcag acttttgatt atttggttga
124741 ttttaaacat ctatagtact catcatataa agataattga gagttggcat tgttcatgag
124801 cctctgcctt atacatgttt ttaaaataag ccttgtaatt acctttcaca aagaggttgg
124861 cgtgagtttt gaaatctttt gctgttagtt ggacttcccc agcatcttct aactttacat
124921 ccctcagagt aagtgtatga acttttcctt ctgaacgtgg gaccacctag ttgtttttaa
124981 aaaaagaata catgaaacag caatctagta tatgggtgca tttaattcat ttagataaaa
125041 ataaatggaa atagagcttt ccaagttgtt ttataatcat ctatttagca cgtatgcatt
125101 caaaaatcat ttcaaccaat agtttgggat ctttttttta aatttattta ttattattat
125161 actttaagtt ttagggtaca tgtgcacaat gtgcaggtta gttacatatg tatacatgtg
125221 ccatgctggt gccctgcacc cactaactca tcatctagca ttgggtataa ggactataaa
125281 tcatgctgct gtaaagacac atgcacacgt atgtttattg tggcattatt cacaatagca
125341 aagacttgga accaacccaa atgtccaaca atgatagacc ggattaagaa aatgtggcac
125401 atatacacca tggaatacta tgcagtttgg gatctttcaa gaaaatttta gggtttcata
125461 gttaccttta tataattact aggtggcctt tcacattgaa attgcccagg ccagtttctg
125521 tttacatttg tggtcttttt tgcatatgcg tagatataca gcacatatag tatatacgta
125581 tatgtgtata tatatgtgtg cccccattct gcccctgggc ttatatacat ataaaggaag
125641 aacttagaaa agcgaattca tgaaaggatg aaagaaaata tcagctatgg caagctaaaa
125701 gtaaacctta tgttctgtat gtaaggtgtt tgccatgcaa caacaagcta taactcttct
125761 tcccatttat tttagcaatt aaacttattt tgcaaaccaa ttaaacctgt gattaaataa
125821 tttgtacaac ctattaattt tgttcaaaac tgcaaatgaa agttggactc ttcttccttt
125881 ttcatgtgtc taagcatcag gaatgggaaa atcagatgtc aatggtgcga cagatagggg
125941 tcattctgac agcaaagata agagacttgg gggttcttcc agttctatga tataaaattc
126001 ctaagaagct gtgccctgtc atccagactg attttgcact gcttaagagt ttacttagcg
126061 ttaaaaataa tgtaaagttt ttcaccagtg gaactatttg ttatgctagt gtaaagaaaa
126121 gaatcagaaa taaggtttca tctaaaagtg ttttctttaa ttaagaactg ggcatggtta
126181 gtcacataat tatcttaata attgagagtg gccaaaaaac ataataagga aaaataacag
126241 ctaggagggg gtagttaaat atgcocttgg aacagagcta cttatccgag ggcatggtgc
126301 tgaaatggga aaaatttgtt tttttaataa catttcagta taaattttgg catgcaactt
126361 ttggctattt gaaaaggtgt ctcagtttct aacccaccca cagatatatt ttatctatta
126421 atattatgtt gctttatcat ctgagttcct ctgtagctac tctaggacac tgctcagaat
126481 gcagtatgtc tcaataaaca gcttgtatac aatctacctc cactatgtta aaagaaaaaa
126541 aaggaaaact ggtaggtgcc ttataaaaat aaaccaatga aatgaatgtt gaagattttg
126601 cagtagattg taagaataat gttagagatt ttaaaaatat ttatttatag tggaagcata
126661 ttaatataat acggtaggct tatgaccatg acaaaactat taaagttttg tatttaaact
126721 ttaattttat caaatttaaa atttaacttt aaaattatca aatgccattt ggaacttggc
126781 agtcctttct taaactgaca gccaaaaaca actacaacaa caaagaaaaa atgcaaacaa
126841 ctcatgtcta caagtttctc ttcatatttc ctattcctac agcttgtttt tgcttgtttg
126901 gttattgtag ttttgaaggc gaaaattttt acataaaatg agtattaaag ttcggaatta
126961 cattgacaag agcaagtttg ttctgacaga cttaatttaa acaaaatctg ccacatgtaa
127021 caaaatgatt ttattacttc aaagtttata tttaagttct ttttgaaaag agacatttaa
127081 gtaatgcaag agcaagttca caaaaggcta aggtatttcg ggtgcctaga gtaaaggatt
127141 aaaattccta tgccaaaaga gatgtaaagg ggccctatag aattatagta aactgatcct
127201 ttctaaccct agaaaaaatt ttagttacaa tgagaatttt ttaaaaataa aatgtgaata
127261 agatttttag gagtctacat ttgaattaaa atagtgcagt ttttgtatta gaactgagac
127321 tgatctttta ataagaagct cttactatga caaaaagata tttggtaatg aaaaaataat
127381 atcatttgtc tttaagcagt gttcctatat ataaatcatg aatttgcaga tctagaaaga
127441 atcttgaaaa agcaaccaat tctctgcctt tagacatgaa gttaattaaa tcatgcatga
127501 taggcaaaat atattttttc aaacaataag cttgaaggta gaggtgcctc cctcagttca
127561 atatttagaa gacctcattg taaggaaggt tttgtgatat gtaacttaat tcccatgcta
127621 caaatggaat gtactgctgc tttttctggt cactggaaaa ggagaccaag aagatcttat
127681 tatctgtata acaggcaggc gttacctatc agatgactgc tggtgaatta ccctgtaatc
127741 actttagtgg aatactagtt ttgttgttgt tgttgttgtt tgtttgtttg tttcaaagca
127801 gttacttgaa ggcattgctt tcaaatactt cagtcaatgc tgtggctttt tgttaaactg
127861 aatcactttt taattatatt cctttaagtc ttcaaagttt gatttttgtt gagatcttta
127921 tagcacattt aatagtattt ctgaaatatt gggggaatat gtcacagaac aatgtactgt
127981 cctgtgcttt atttccaacc caggctggac atattccata tattttattt taagaattca
128041 aaattaatga tggattgtca agataattta aagacatatg ttgtttctgg ggacaaaaca
128101 tatagatcaa tttaggcaaa ttgtattgtg ggcctatctg aataatccct tagtttttac
128161 ttatgggtaa aatcttgggc ataaagaatg ttagtaaaag ctaaaagcag gagctagtta
128221 tttaccaaag cttaaaaaaa atatttcatc cacatatgtg gataaccaca gagcccttct
128281 ttcttgtcag taccatcagt tgcactttaa atagtccact tgcattccag atatcgcaca
128341 gcccagagca cttgaaggtt aaaaatagct ttggagaaat gcttgaagag caaatctgcc 128401 cagaagttat ctcaactact tgatcaactg cccgaaatgg aatttcagtc ttctcctctt
128461 aatcccctta tatgcagaac actaaatagg atggagagcg ttagtcttaa aacttaatgt
128521 gcaccatggc tgcagagcgt gcaatcagaa agcctttttg atccttcctg ccttctggtt
128581 aatcaaacta tcattcatag tcaatgagtc ttgtcttttc ttcaaaaaac taaaacatga
128641 tcatacttta aatttatttc aatttcaata aaaaaatcat caggttaaac ctaagaaaaa
128701 atgcaaaaaa ttggctttac agtttcagca gaaatcaggc taaaggcgga aagagaaagg
128761 caaagacaga ggaagagaca ttagaaagag caaagaataa ggaacataca agtgaaggtg
128821 gaagaactcc agaaaaagaa cgggaaagac aaggcatgcc tgctttttac cttatcgctg
128881 ggctctagtt tcttcccttt gagataccat tccactggga tatcttcgta ggagagctcg
128941 cagtcgaagg tggctgtttc ccctgcagtc acggtgacat cctttaaagg cctcagaaga
129001 ccaattactc gtgcttttcg agagggaaga aacagctttg cgttactcat tttgtaatcc
129061 caaaagaata aatagagact tagatatgaa tcttcatggt tgctttcttc aagaagccac
129121 aggactggcc actattttaa ctcccacgtg aacgtggccc cacggcgctg agaaggggag
129181 agctggtagc cagagagcga gtatatgtag gggccctttc gttctgacgt acaaggtcag
129241 cgagaaatct gtgctgcatc tgtctctctg ccaggcgtca agttcttctt gctacataag
129301 gtatgtgact tccaaaataa ctaagactct gattgggaga ggacaagggg ccctcaatca
129361 gaggctgtcc ctatccatca aacactttgc accttccaga aacgttagcc taatgcttcc
129421 caccttcttt cttgaatcta aaatgcccac acagcaaagc ttgtctttct ggtgctctag
129481 ttgcacagcc acttgaggga agtggtcttt aagggttctg agttagattc tcattgtatg
129541 tgtatctact ttcaatgaga ggactacaga gcaggaaaat ctgatttgct aaattttctt
129601 ttgttcaaat ggcaaggtca acaaaatttc caaaacttta cgatttgaac cacttctgta
129661 ttggaatgtc aaagtggcaa atacaagaga gccagttttt gtgttttaat aatattttct
129721 aactaataaa ataggtccaa ttaatttcac tcacattcat tttctgaaaa agtgtttatt
129781 taatttccct gaaaaatata caatacttac gcttaactcg gaggtgggca ctagatttaa
129841 cattggcagc ttggaaatcc accccacccg tctggtccag gcgacagttg tgcaaaacaa
129901 gggagtgtat tttgccttct tccttaattt cacaatcctg taccaacaaa acagaaaaac
129961 tttcatagaa aataattttc tttgaaattt tgttctaagg atagattatc ttaattaaag
130021 gaatgcaaca aataaatggt gatgaaactt tatataactt tgagctcttt ttttaggaag
130081 taaagcttac aagtactaag aggaaagcca catttatttt agctgtataa ttctgagcca
130141 actagcagca tcacagagta tctacaacct tatactcact ctgctggatc aaaagtaatg
130201 caagtgtgcc agtctgagcc cattcataga ccctgacatc tctctttggc ttagggtctg
130261 atagagaaac tgctatcatg ttttaaatgt cctgcatcca ctctgacttt cacctgttct
130321 tttctaattc acacagctcc tttaggtgtt gacaagttca gaaatagcct tacaggtgtt
130381 tggagtaggg cctctccctt gagtttccag ttggcgtgga tatcatcttc agagatttcg
130441 gtttcaaagc gtgctgtctc agtctccatc acctccacac tgtgcagggg tcgcaccagt
130501 ttaatttccc gatctagaaa agtgaagggc cagcatgggt cattagcctc tggcacaaat
130561 aaccccaatg cttcaagagt gaaactcatg gaaatttcct taccctcaat gtcaagtttt
130621 cctgaggtct tatctgtccc acagtcacag gtgtactgtc caatatctga tttcaaggct
130681 ttctttagga ttagcatgcg tttcttgcca tctgccttaa taacagcatt tttggatggc
130741 tttatttcct tcccatcctt aaaccatttc actggtgcat ctgctttgct aatttcacac
130801 tgtagaataa cttcatcttt ctctacacca gtataatctt gaagttttcc tgtaaaatat
130861 gggtctccct ctgcaagtaa agtataagtg aaaagctttt attaatcacc ttagggaaat
130921 gacaatctct tggaaagttt aagacacaac tcacagagtt ctgagtatct tttaaaattg
130981 tgtcaagtgt tcaatcattt aacctgttta tgttcaaaag ttctctaaat attaggaagg
131041 gaatcagtgt gtgggattta tcaagatcta atccattcat attgaatttt gaaacatttt
131101 attaacagta gctaaaaaga ctgaaattta gcttcaaata caattttgtg ttcctccatt
131161 atttgggtaa atttctaatt cttagcaact ctattatgtc tagtttactt ttaagctttc
131221 aggttcacat tgttagtttt tcttaatcaa ttaatagttt gcctttaatc atgtctatat
131281 atgttagcct tcatgtttaa tcttgttgat attaggtggc caagaaaaac aatgaggctt
131341 attaatgtaa attatgtaat atcttctctt atgggaaact tgaaaaacat tcctctgaaa
131401 taattttgat aaaatgaaga gatagaaagt atttatatac tcctatttta aacatgacag
131461 ggctcaataa ttaatgaaga attttaagtt ggcttttaat tggctatttt gtgggtgtaa
131521 gctagaatct tcattaactt atagtaatga aggtaaagtg ttttagaaat aagcctgatt
131581 acatatgtga tagcttctta aggagttggg ctgctttcat gcaatataac acttagaaga
131641 ctattttata gaatagatac tccacaaatt tctgttgaat ttaatgcagt aaaattaaaa
131701 tttaaaaagc acttaccaag cacagtgagt ttagcttctg aactcatccc catagcttct
131761 actctaattt gggaggtgtc atcaatagac aggtctttga atgtgatcga atgagttttc
131821 ccttcgtεtt gcattgagac cgacctggtg gtgtgtaggc gctggtcatt cttgaaccat
131881 ttaactcgga tgttatcatg agataactcc acagtaaata cagcactttc cttctctttg
131941 gcagttacat ctttgagagg ggtgaggaat ttgagccgga ttcctatcaa gaaaaaaaag
132001 aaagaactta ttaattgaag cactttaaag aagaaatata aaactaaagg caaaaaaaat
132061 gattttgggg tggactattt gataaaacta tttaccttca atgatcagtt tgccacttgt
132121 gtgcttatct tcagcttcaa acatgtattt tgcttcatct tcaaaagcag ctgacttgat
132181 caccattgaa tgcttagtgc catcctttat aagctcaaat ctgtcatcac ctgtgatttc
132241 ctgggttcct tttagccaac ggaatgtttt gggctccctg gatacttcac actcaaactt
132301 agcctcatct ttctcgaaga ctttaacatc actgagaggt gtgatgaaga taagaggcaa 132361 ttctgaaaga agtggacagt ggatgaagtc agaatacgtt tccattgatg acccttgatc
132421 aatgcagggg tgtatcagga agtcttgcag agaaaataca aaaacgtggc tgacaagtag
132481 agcatacctt tcactttcag attggctgca gatttggcat tagcagcctg gaaggaaacc
132541 tctcctgtca tacccagctg acagttatga aggatcagaa tatgcttctt tccatcctca
132601 atgatttcac agtcctgttt ggagtgaggg ttagaaggaa agaaagaaca tcatttatat
132661 agttattcct acaaatcatc aagatatttt atgccttttt tcaccctaca caaccaagca
132721 acccctctcc tatataatta gctaatcttg acttacaggg gaagctgtca aaggctgtcc
132781 tttcagcttc cactggccgt gaacatcagg ttcagaaagt tcaatttcaa agtgggctgt
132841 ttcaccaaca aacacctcta ctccgtacag aggcttttcc acttttatta gtcgagctga
132901 aatgatacag ttttgttagc atgactgaac taataaactg cagattcaga tagggtaaat
132961 tttacattgt tgagcaactc accctcaaca gtaacatttg ccttggtctt gtctgtgcca
133021 cagtcacaca catattcgcc tttatcttta aggtccgcct ttttgatttt taagatgcgg
133081 cgcaggccat ctgccttgat agaatatttg ggtgaaggga caatctcttc accatctttg
133141 aaccatttca ctggtacatc tttgctcact tcacacttca aagtaatctc atccttctcc
133201 actgcagttt tgtcatgtaa tttcacagtg aagtagggat cggcctctgt aaaagacatt
133261 tagcataaaa ttagaagaat gtgaaaatta aagtttatta aagagtaaaa ggttgcaaaa
133321 tattttatta ctccccactc ccatgattca gaaactggct atctggttat acttggttac
133381 atttacctaa gaccttcagc tgtgctgtgg agctcagctc cttgacttga gcttttatct
133441 gagatatatc atccaatgtc acttctttca tttccaattt gtgagtcttg ccctcagaag
133501 agatgagtac tgttctgctt gtatggagtt tggcatcatt tttgaaccag actacatgca
133561 ttttttcatg agaaagttca caaacaaaag ttgctgtttc accttctttt actgtttgat
133621 cttcaagagg tgacatgaat ttcagtctta tacctgaaat gcaagcatag ataatgcctc
133681 agaaacacaa ttcaccttca gaaagattcc attctaatct gcctgagtaa aagggaccca
133741 tttcacatgg cacttattta ttcatctttc caaatagagc tccactaaaa acaaattaag
133801 gggggttgtt ttggtaacac tgtgaaagtt aattagtga't gcattatcac agcttttaga
133861 acttggcgtc ctatctttaa agtcatatat ttgcatgcct ttatgggatg tcacagatct
133921 cattagctcg cttacctgtg acaaacaacc gagctgaggt cttcttgccc tccacctcag
133981 cagtatagac cccttcatca tcaaattgag aatcattaat aacaagaata tgtttctttc
134041 catcagcgat gatatcaaat ttgtcagatg acttaattat atcaggtcct ttggaccata
134101 taacatttgc ctctcgggtg aggacacatt cgaatcgagc ctgtcgcctt tctggaacag
134161 tgacatcctt caggggcaca gcaaagtcaa gttcgatttc tgaaaatcag acattaagaa
134221 tgaggctttt cagaatgcac agggaagtga aataaagttg agacccctcc ccaaattcta
134281 aaagccccat accttttact gtcaaaatgg cagttgtaat tgcattaagg gcctggtaga
134341 ggacttcacc agcttggtcc agtttgactt tgYgcaaagt taagaagcgt tttccacctt
134401 ctgctttgat ttcacaagtc tgaaaaacaa tagttttagt aaccatttga aagagataaa
134461 ttccctatag gagaagtgtt tcagatacaa atttctatag agttttaaaa attactacta
134521 ataaaagtaa gcagagaatt ccctgtcata acattttaca caaattgttc aagttgtccc
134581 caaatgatac gtaaaatttc tttccttctt ggagacttta Raagactcca ttaactccat
134641 tattattaaa gcaacccgaa tctaggatat agatcctgaa tattggatgt ggttatttta
134701 tatgactaac aatttaaaat gtgaaattac tcacaggtga gggccttaga agttctcctt
134761 tcagtttcca ttgtccagga atgtctgcct ctgagatttc tgcatcaaag cttgcacttt
134821 ctttctcata aatggtaacg tcctctattg gtttaagcag ttcaacttca cgctctgtat
134881 tggtcaggga tgaagtaaca taatgcttta gacaacccaa cgatgctttg cttttatgtg
134941 ttttggtgtg ttatttggct ttacacatac gcaaaactta taatttgaat aaaaggtaag
135001 attttatacc tccaagtgtc agctttgcag ggtatctttt tccttcaatt tccactgcat
135061 actcagcaat atcttctggc atagcattct taattttgag gtacagatga tttccatctc
135121 tcagtatttc agtcttatca tttggttccg cagggatttc atcatatcct ttgaaccact
135181 taatgtttgg agtatctttt gctatatcac aggcaaaaat agctgtcccc ttgggtttca
135241 catgctggtc tcgtataggt ttcaccagcc aatctctaat gacttctata tgaaaataag
135301 atcagaaaaa atgattaagg tctgaacaag taatatacat agcttcctta gtaaatttca
135361 ggaataggaa aaatttcaca atactgggca taattaatcc actgtaggat atattgttat
135421 tcccctctta ggtacaagat ttctgatatt gtaatactgg gaaacgaggt cctttcttcc
135481 attttcttag tgtaacaata agcagaacga aatctcccag gaaagtacta acctactact
135541 tttacgcaag atgaacactc caggttgttg gcgttttcca cagtaactgt gtatgttcca
135601 gcatctgtgt catctgcatc gttgatggtc agagcccgca tcaagccaat gacgcctggc
135661 acaattcggc caggtttctc caccacaatc ttgccatcct tcctccagac cacgtcacgc
135721 tctttgttta actcgcagct caagtacaat ggctgtcctt tgaccactgt gacttcctct
135781 tccagtgttt- ttacaaaacg cacaggaagt tctatggaga atttcagtat atatttcaat
135841 aaatacaata ttttcaatga aataaaactt ggaaataaga ggttttgtaa aactacaatg
135901 caagttgcta ctaaggtttg ttacattaaa gtttaatata gattatgttc agaagactag
135961 aaattacctt ctacaacaag tttagccgtg gaggtctttt ctttgtttcc caaacgtaaa
136021 acacaggtgt attcaccagc atcggaaagt tgaacatcaa tgatgtgcag ctttctgtct
136081 ttaccatctg caataaacct gtgcttggga ctctcacgga tattgctacc gtctttcatc
136141 caggttgtaa ttgcagtgga aggggagacc aaacattcaa agattgctga agagccaacg
136201 aactctgatt ctgtcaagat gatgtctttg atttctttca caaacttcag tggcacagcc
136261 tttagctggt aggtgaacgg ggcttcatca ggaggtttct ctccaccact tcctggcctt 136321 aactttctcc tttcggcttc tattggtgaa ggagtctttt tgggtacacc taattcaaag 136381 taaaataaaa agttgatttg gcatctcctt aggagtcaga aagttcatac ttggctgcct 136441 gctggataaa accagccgta aagcaattag aagacgagaa aactaaagac caggcaattg 136501 aaaggccaat tgagtttata ctgccttgtt tcaggcagga aaatgagatg gtataaggaa 136561 tccatgaata ataaatgtcc cttttctcaa gttttaatct gaagtataaa atagccagat 136621 taatgtctaa ttattttatc tgccatgtat aaataccatg catatgaaaa atgaagctaa 136681 gaacttatgg gattcactat gctaaaatat agttggatat atatatatat atatatatat 136741 atatatatat atatatatat ctccttgcat aatactaaaa gtattatttg aaataYcaga 136801 aaacatgcta gttttagtag aatcaattag aaaaaataaa aattgttatg aattttgaaa 136861 tttttttccc cttgaatatg aactttggaa attcagagtg gaaggttaca atgcaagtac 136921 tagaaaaatg aatttcacct ttgataggac cttttggctt ggcagcctct tccttaggtg 136981 ctttggcttc tgaaataaaa ataaaactca gcatttaaac acttttcgga cttatttcat 137041 gtttaatagt aaaccatgga gggtgagtca tgctccatta taaatattaa cgcataaaga 137101 gaaattggtt tcatttatat gacttctatg gataatcaca tttccaggaa ggcatattct 137161 gagactgaag agctcaagct aaactgccaa catatttagg attaacaact tctttcagca 137221 agtgccttat tRtcttccca tttttggaag gcttactgtt gccttgtgag gaaaagtttt 137281 gctcctgcct agctgtgcaa aatattttat atgtttatgt aagtcctgat tatgtgaacc 137341 aaggaaataa ggttttgtca ataaatcagt gtagggaaaa gggaaatggc tgagaggtta 137401 ccaaaagcct ttctttttat gtctaaattg ggcagacaaa caaatccagg gaagactaag 137461 gaaaaagagt ccatgtacca cacatgcatg agtttctttg aagatttcct ttagacaatg 137521 aagtaaggca gaagcaagag agttctgaca tttagagttt gattccatta ttaatacagg 137581 gacagtataa actgtggaat gagttctcta gcttcagccc ccaggataac tgagaatagc 137641 tttattttta tacatactta attagacaag agcagttcat cacctttttc tgaataataa 137701 aagtactacg ttgacattat aaaagtttaa aagaatatgc ccaaatcata aacatcagca 137761 acaaaatata acacaaatat taaaaattaa actacagtca aaagaatata gtgtattttt 137821 ttatgtcatt aaacatggta atgtccaaat attttgagat aattttaaat aaaaaaattt 137881 ctaaataatt tatataaaaa tatataaaca aatataaaat gactaattat tgaattaata 137941 atctaaaata atataatttt tccaaatagt tttatttatt gatttattaa agatataagc 138001 caatttttat tattaggttc agaaaattaa attaaaaaat ttctctgtca aacWattatg 138061 gacatttcta aatgttattc ccggggccta tgcacatctc actgtggaat gtaacaaata 138121 gttctctttt ttttttttta aattcaccct gaatttttta ttttgtgctt tatcagtgga 138181 gatgatcatt tttccatagg aacaaattgt caaggcatta tattgaatat cttagattct 138241 tcacatctta gaaaattatc aatacatgcc ttctccccac cccaccacca ccgcccccgc 138301 tttaaattta ctttgtttta tttttggttt tgttgtttct tttttaaaaa aataatttca 138361 acttttattt taaactcatg ggatgcatgt gcagttttgt tatgtggata tatttcatga 138421 tgctgaggtc tgggatatga gtgatctcat cacccagata gtgagccaaa gggcaaatag 138481 tcaggatgtg tatgtttatc aatgactatc aattacacat caaatcagtt ttgatcaatt 138541 atatctcaaa ttatcactta aatgatgaac tatactaaat agttcatata caattaatag 138601 gtgctttaag tttggacttg agatacctat atcattaagt tagaacattt aagaaaatta 138661 ttattgatct aattccttat acagattaaa agactgaaat atggagtctc ttttttcttt 138721 ttctttctta ttgtggtaaa atatacctaa cataaaattt accattttag ccatttttaa 138781 gtattcaatt cagttgcatg aaatacattc acaatattgt acaaccatca acactatcca 138841 tttccagaac ttttacatta tcccaatata cacacttttt taaaaaagag aaattgttcc 138901 aatagggtat aagggaaatt tgaagcttca tgggattctt atatgtatac ccttacaggg 138961 tctctgcctg tctccttagt ttttatcagc cccaaacata ttcattactt aatctccaaa 139021 gcatatgaaa gaaatggtgc aacccaggaa cgtacattcc agttaaggca caatattcac 139081 ccactgggca agagacaagg tggataaagg atatcacatg aagaaaaggt agaaacttac 139141 cataaacctc cgaagttgaa atgataccta tgttgcagca taaacagggc ttgaatttta 139201 atcaagtgtg aatactttta ttaagtcacc aaaacaaact agcaaaaaga aagctacagg 139261 ataaatacct gctttctttc caaccactgg cactgttact ggggcagcga tgggggttgg 139321 ttcaggttcc acaggaggtg gtttgattgt tttcacttct gtagagagaa gtccattgca 139381 ttagtgtatc aatttgtcac ttcctaaaaa cttatttggt agcttatttg cctcttctga 139441 tataattttg aaatcttcaa ataactagtt tttaagagaa tagtatatta agcattttga 139501 gacgttagaa atcatttgat acatactgac tttcacctac tatcttttat taagtacatg 139561 ttatgtgtgt gaatacagaa agaatatgct gttgacattg aggataagct tgctcacctg 139621 cttcgggctt tggtttcggt tcaggtgcag ggagaggtat tgctggcttg atttcaggca 139681 ctgaaataat ttagagtaga gggcagatta ttacaggatt tttgaagttt ttcacaaagt 139741 caaaactaaa attaagccca cgtatcttgg aagatattag aatttatata catataatta 139801 tcaaatcaag tatttgatta gttttcattt taaacaaaat atttggaaaa tatagtaagg 139861 caatgttctt ggttttaatg ttgttattta tctcattgcc cataaataca attttttttc 139921 aggtaactta tttagcaact gagtaatcat tagccaattg cataggagag tgagatggta 139981 aagaaaatta agccatatgt gattaacaga ttaaggtcat gtgttcaaat cttgatgtca 140041 gtgtaatgat attttaaatg atacatattt gcatttttag agacaactaa gaatgacagt 140101 agatttgtac cttttgttgg ttcaggaatc ttcctttccc tttttgtaac agtaggtact 140161 tcaacctctt caacaggttt tggaggtggt ggttctggta ctttaagata agattatttt 140221 ttcagtgtta atatttgaat atttaggaag cacctcatct gaaatatcaa tttatttttc 140281 aaaaactcta tggatggttt tttaaaaaac cttatagttt atcaagtaat ttctatttta
140341 ttttcttgac tctgcttcca tttatttttc ttccttttat tccatccact tatcttccac
140401 tccccttagc tctctccttc taaatcactt ctataatagt tcccaataat tttgcccttt
140461 gagaataggc taatgataaa aaccaaccca tttaaaagcc cccagattat gaaacctttg
140521 ctgacaccct cccatgaata gccacttgat tatttcagtc atttctactg gaagaactta
140581 agttgcaaag taatgataag aatgaagtgc agggaaatac atgaatggat ttgggaatta
140641 ctataaaaat atttaaacag aaagcagaca atggaaaaca gagactacat cacagatgaa
140701 atatgaagcc atgtttacat ctaacttcat ttatgtttRc aagataaacc ttttgttaaa
140761 tgtccatttt gttaaaagat aatcttacaa attgtcactg agattcctac ctgcaggttt
140821 tttaactttt tctagttttt taggttctac tttaggttct tcttcttcag gtctttttct
140881 tagaacttta aagacaaaaa ggtttatatg taaaccaaga caaactaatg aagatgttga
140941 ataagcttga caaatgcaaa taatgtacaa agcaaggaaa atgaaaaaaa gcatgctacc
141001 tagcactctg gaaagtaagc atttacaccg aaagtgtttt ttgttttgtt ttttttaaaa
141061 tcaaaggttg tcaattataa tagcactcaa agttactttg tttctatcta gatagagggt
141121 ctattctgag acaagtctgt gggagaaaca gctccaacac aaaatgaata atttgaatag
141181 caacgaatta taataataat taaatgttaa tgatttaatg agtatgtaaa taagtagaaa
141241 gtttacttaa gcagagatat ctgaagaaat gtagcctaaa aatcagtaat tctatgtgaa
141301 gacaatgtta gaacttaatt cttcatttga ctatcttgtt tctcattctt taatatttta
141361 ttagtcagaa agaaatgttc agttttttaa aaatgttatt tattagttgt ggtgatcctg
141421 tgtcacactt taaaactgaa tattgaattt aagctaaagt agtttcatgt aaggtattta
141481 ataggtttag tgtactttac tacataattt ggttatgaaa atggcagtca tagttgttgt
141541 tagtttagaa tattatcaac ttagttatgt ctattaacat tttaaatttc aaaatactgt
141601 gaatgctcac aaaatttctg tctttttttt ttaaaaaaaa agatctggaa atgacatttt
141661 tcttaatttt caatgaaact acaaacaaat tttgataaat agcgaaccaa ttcaaagaaa
141721 accaaaggac agaaacattt tgtaagcttt caagttcatt tttaaaatat acttaacgct
141781 gacagaatgg ttgaaaaata ctataccgct tttcagaaca acttcttcct ttggttcagg
141841 tttacgttcc ggaagtaatt tgcgaacttt cttttcacct ccaggcactt aaaagaatat
141901 gatttcaaat tttgaagtga atttatataa aaacggaatt tcaacaagaa aaatRtctct
141961 cctaaaacta gttaactgta gtgcattaag taacaatttt cgctgcatat aaataccaca
142021 gaatttaagt ttaaatgata catttcaagt tatctcctct gcaaatatct atacagcctt
142081 gtgatgaaat tcaactccat taactgcagg caaatgtatt cattttaaat gcatgtagag
142141 gatgttgctc catactgact ttatgaatct ttcatcaaat tacagggttc ttgacagcat
142201 ctttgtccca aactgcaatt gtagattgtc caaatctacg atgttatcta cccaaccact
142261 aaatgtatga aaatgatact ggcaagtgtt agtgccatca ttcagtcttc tccactgagg
142321 gaagaaggtg gccaagagtt ccggtttgtg actttgatgc tggggaaggg ccatatctca
142381 ctttctgaac agaagaagag tagcctttct taagatttcc tttttgaaat aatgctggat
142441 aaaatattta aaattataga attataaaat ttcaaagctg gtgttggaag ctcgtttttc
142501 atgaataaga aaatgttctt aaattcattc aaattaagga acgcaaggaa ttcaaaattt
142561 cctgttgcgt ctacagtgaa tactgagcat ggtggatgat gctattttat agagcagcct
142621 cttttgctat tatggccctc atagatttga atggtgaaag aaaggaacac gtggtgatta
142681 ttaaaccatt ttcctctcta ctaatgtcaa ttaaaatcta atttaaaaca gtatttattt
142741 gtattgtgta actatttgct attaggaatc tgaattttta gatatttcta taaaacttcc
142801 tatttagggc tttaaacaag atatttgcaa ttcattaaat ggttttttat gtgataaaga
142861 taactgctta actcttattt caaaaattta aacctacaat aggcaaatat gtatttcRtt
142921 ttgaataatc atttttttaa tgtcaaatat tttaagtaaa aattttaaag ttaaacatta
142981 cgtattttat ccaggataaa ttgtgttggc cttcaatact attttagagg taccaatctt
143041 cttaataact atttttattg ctcaaaccca gaaggaagca catgcatcta attaatgtaa
143101 agtgaggagg cataatatag attttcttta ctgatataat gaacatagtg aaaatatatc
143161 tgtgcatttt ttatattatt atttgatttt aaaaatctta ttgatgtttg agaacccaac
143221 attgtagcta ttatttttaa agtcattata tgtgattata tgctatttta atgtttatga
143281 tcaatatcca tgataacatt attttgtaat tgcctcttta tgaaatcagg aaatgtatat
143341 ttatatgggt gacttttttc cccaaaattt cagagtgacc agaaacagtc ttaatttcta
143401 ggtctgaatg tgttgattac ttgaacagaa cactactacc cattagaaaa attatgttta
143461 tttttgatag atgttttcta gactcttata attcatttag ttttctttag ggtctaattt
143521 tagatgagac cttattaggg aaaatattgt ttacagaaaa atacattttg aaaaattacc
143581 tacagtaata aggaaatttt tctgaaatgt gagttccgta cccctcagag aatgacttag
143641 agccagtgag aagagagtga catgaaagtc ctaaggaaat ccctacaaat aaccttaata
143701 atataaaagg aagataggac ttatttttag tgcttattac attggctaaa atttttatgt
143761 caggtgaaat gtcaaaacag tttactacta gctctcagca ttttaatctc atatgtctct
143821 tgatatcaat tttggctaac cagtaaaatt tgttattttt aagtttagga aaatgcaaga
143881 agacatatgt agatgagaca aagatagacc atgtctatac atgtatgaaa tggatgggag
143941 acaaaggaag caacactcat gagccaggac cataaaagcc acagaaatat aagaaatgaa
144001 tgggactact ctctgatcat tgaaaaagag agacagaaca ttcgatggag gcagaaagag
144061 caaggagtca ggtaaagggg tactacataa gtatgtattt ttcagcctgt gaaatacctt
144121 tcagaggtgt aagctccact ttttctggaa cctgaggttt ttcaggaact ttcttctttg
144181 gaatagcttt aaagaatatg attttacttt tgttatttgt atatttaatc tgaagcaagt 144241 gattaacttt ctactccaag aatcaaaacc aagctttgct tataaccaga aagcattaat
144301 aacagccaag aacagccttc aaagaacata caagcagcat tcgaaccatg aaaacagact
144361 gcagttttac tccaaagttc agtagttaat taaagaccag cagaaaaaga agacaatttt
144421 tgttattggc aggaggaaga aattttcctt taagcatata ttttacatta agggaaatgt
144481 atttatgtgt gtgtcttcat ggagtgtcaa ttaacagatg ggtggccttt caagaggtgc
144541 cagaaggtcc ccctaatggc tatgagggtt gtagagggca agaatcacca gcagcaatgt
144601 cttatgctgg aagactgtgg tttgaagata cataggtctg tatagggaaa gtgcatatct
144661 aagattatat ctagataatt ttagattggg gaatcatatg acgtcacaag acaacttttt
144721 attaaaataa gaataatatg tttctgccag tttatttcag cttttaaaga aactgatatt
144781 tttaaaatta agtaacattg tgtcaattca tagaaaaatc aaaatataag aagggggaag
144841 ggaagtttaa taataaatgc tggataccaa atgtcttcat aaccactctt tccagctccc
144901 ctgagaacca cacagaacac ttctttaagt tgcaagaaaa aaaaagagat gggggaactg
144961 taacaagtca ttcatatgct ttcctttcat gatatatagg cacatatcag ctactggaaa
145021 tcataactat attacttgta atatatttaa aagcacatgc aattctttaa gaacaaagtt
145081 ttggctttta tgtaaatgtg ttactgcaat attaaaaata tcttgcaagg atttcttata
145141 cattccttaa gcataagatc gcaatgatat gtatagcaat caggctttga aatcagaaaa
145201 ctctttctcc ccactccaca aaagaaagtt aattggacag tgcctgcatt cagatatccc
145261 ggatgacgct atcaatgtac tttctgactt ttgcagcagc aggcatggca tgacaagcaa
145321 cccaggaacc atggggcagc agaagaSaca gctctatgca atctcacagt acaatcatgt
145381 ctgaaagtac acacttctaa aagtcataca tttcttgcaa gcatcatttc agatggctgg
145441 atagaagttt gaagcaggct aataaaactt tggaagtggc attttttacc tttaagttgg
145501 aatattttct ccttcacatc ttccttaggt ggagcaggtg gaggaggtgg gggtcttggt
145561 ttgagttttg gcttctcaat aaccttttca ggttcaggtt cttgaaagag tatttcagag
145621 gtgttagtat atgtatatgt tagcatgtgg atatgtagta tatttaatag aaattatata
145681 tatatatata tatatatata tatatatata tatatatgaa gtacaaagtt tactttttaa
145741 ggtacgtaat accagtatcc aacataaaac acagaacata aaacataaaa atatcaaccc
145801 tttacaaaga tgatgagaaa tacagcaaag gcaccaataa caacaacaaa caaagcatcc
145861 agacaagcca cagttgacat gagagaaaca gtacaagtta catggaaacc taagaatgag
145921 gctcacattt acaacaaaga atactttaca tacaaatggg aacagacata ctacatatgt
145981 acaacaacat aaaccatata catttcaaga gaaagaatat gataaagaag atttaagtcc
146041 actggattga atacctttta ctggtatttc ttcaggctgt ggttcaggtt cgggctcttc
146101 aggtttaata tacttttcaa tttcRcgttc tttaaagaat gttgacaaag gagatgaggt
146161 tgcaatgtaa agttcttcaa aaagactcat acaaatttca cattgatgca aagattacag
146221 tcacaaaata aaaatataca attcatacaa attcatgtat agaaaaattc acgtataaga
146281 aaaagttctt ttacttgaac tgcctattaa ttagattatt caatgtatat tgatggcaaa
146341 tgagatgact tttaaaattt gataccgtgt tatcatttga ggatccaagg caattattcc
146401 attttgcttc agtaaactgt accttttgga aattgtaatt accaacaaca gtaactaaat
146461 taaagattct aacttaaaag aagtgtaact atttcaaaaa taaagcagtc aaacatgcta
146521 gactgctctt gtatataatt tcaagaagga atattgtcct gtaaatgctt ttgtaattct
146581 aacaggaatc ttcaggagat taaaaaaatg tatatatata tatatatata taccttcaac
146641 agggggagtc tcttttctac caatggttat agatgctttt tcttcatata ttatttcctc
146701 aggagtctct tcaactttaa aaaacatagt attttatttt agactatatt tagaacagaa
146761 tactgcaact actattctaa catatcaacc tagttacatt aagtaacact ctataacaga
146821 ttattcagat gaccccccaa atatcaataa cttcaataca gacagacaaa tacgtaagat
146881 tcatctacaa tcaaagcaag aggatgaaga caattgtcat ctttccaaga tttatggaga
146941 agccgtgtac cttcagcagg tggaacttct ggctctgcag ggataggcac agacacttcc
147001 ttttctggga tgattttctc aggcactttg ggcactttaa agatatgatt ttgtttacta
147061 ttaagaattt agaagacatg caagattgtc taaagagcag gcaaagaatg caggggcaag
147121 agcttcatct tagatttcat ggggaaaatg gcttctgaga catggcacca ttttggacat
147181 cctaatttta tatatgaatc ttctcagctt tcttcatttg cttctagtcc tctgtgtaca
147241 cttaaatgaa ggtggtctaa ctttgacact taaaaaaatt tgctctacat tttctgagta
147301 attgttcctg cacactcaat tcttatttac atgtgaatga ctttaagatc tacatctcta
147361 ggcctgatct ctttcttaag ctcctgatac acactcccaa cttttatgct ttaaaggtga
147421 aaagcacatc taccattttc ctctaacgtt agttcagaga cccaatgaac cattcttttg
147481 gccatctagc tttaaacctt acaatgatct tgggctcctc aatttcccta atggttttga
147541 taatggtcag aggccactaa gtctccatct ctttcaaacc cagtccccct ttccatttct
147601 gccgccacgg atcttgtcca actcttaaag accttaaata attgcaatgg gtctttacta
147661 tttagtagct gaaattgctg ccagttatct ttctaaagcc caaatcctat taatatgtaa
147721 gcctgcttca aaatctttga agcttcctat tgcctacaga aaaaagtaca agccttttac
147781 tggcatttga agtactccaa aatatgaccc catctttata tctggtatta tctttcaaga
147841 cattccacag ccaatccata ctcaaattga gtttacttga cataagttta cttgacaatc
147901 cttgaacatt ctagaccctt ctatctctgt gcctttcaat atgctcttcc ctttccatgg
147961 gatatcatgt cctcaatttc ttttttttaa tttttttgag acagaggctc actctgtcac
148021 ccaagctgga gtgcagtggc attatctcag ctcactgcaa cctctgcctc ctgggttcaa
148081 gcaattctcc tgcctcagcc tcccaagtag ctgggattac aggcacatat caccaccccc
148141 agctaatttt tgtattttta gtagagacgg ggtttcacca tgttggccag gctggtcttg 148201 aactcctgac ctcaagtgat ccacccacct tggcctcccc aagtgttggg atgacaggcg
148261 tgagccacca catccggcct tatttcctca atttctacat gtaaaagtcc atcaaaattt
148321 aaattcagtt taacacagct tcccatgatg cctctgcctg aacttaatct tttcttgccg
148381 tgtgtttctg cagccctttt gtattcactt gctatgtgta atcacttcct gtctgtatta
148441 gagtcctgtt atctttctat cctctcacca gatttaactt atttgaagac agaatcaggc
148501 cttaattttt ctaatatgat gtcttgttaa tggtcaacat tcaacaaaat atgttgaatt
148561 gagctgtttt acacattgaa tgagagaagg ttcttttcta ctagttgtag ccatgtgata
148621 tatcacagca caactgaatc ccaYtcattt tttcactcct gcttgtctgt ttcatttttt
148681 tcccttcatt atttcccttt tttctgtgca atatggtttt aacataaatt cacattcagt
148741 atgtttttct ctaagaccta ttattaacat gcttaaatag cagttagaga aatctatttt
148801 ttcttcatgg taggtacctt tttctggaag aacttctggt tttttggtaa caggcacagg
148861 ttctttcttt actggaacaa gtttcttggg cacctcaggc actttgaaga tattagtttt
148921 gttttaaaaa tagtatttaa aaacatttta aaatattaag aataaaaaac ctgtatttat
148981 tggagcagca ttaaaattaa tgaaagcaaa ataaggaaat ttttgtcctt agttatgcaa
149041 caacaatgag gacaacttat tggattccac tttaagatat cagaatactt tcttttttat
149101 gatgccaacg atgaagtgaa tacctttagc tgctggtgtt tctggcttct taacagttgg
149161 gaccttcttc actggaacaa ctttctttgg catctcaggt tctttaaaga tatcagtagc
149221 atttaataat acaaagttgt gagatgtaag atatacatac aaRtttattc aacacYgtaa
149281 catataggta agagttaaca aacatataat acaacacaac acacaataag aagagtgtaa
149341 aattgtagac Mccacaaaaa tgaagtattc attttaacat gagtaccttt aggaggcggt
149401 gcttctggtt ttttgatgac aggaactttc ttctctggga tgatcttctt gggctcttca
149461 ggcacttgaa taataggaat ttcttttaga attaggtgat tacaatgaaa aatttaatgc
149521 taatgaatga attaaaaacc acacatattt cttggtatat gtgggaccaa attctgtggg
149581 tccaaagttt tatgtgtaga aataactttt gttcttaatg tagtcagatt tcaggcaaca
149641 agatacaaga ctgatatttt gccttaagga agatatatag acatgaaaaa tgaaatgact
149701 gtattttccc atacagtgga ttctgctttg tacctgctgg aggtggaacc tctggttcct
149761 cctcttctgc aacaggaact ggcttttcct cttcaggagc aatttcctct tcaggagcaa
149821 tttcctcagg ttcttcatat actttaaaga tattagttaa ttttatttca atgtatggaa
149881 caatattcta agatggacaa acactaaaca cgataaatag taatcttgat ttcttccttt
149941 gtttcaattg ataccttctc ttatttttgt ttagataaaa tctatctcat ttgcactgac
150001 ttctttgtct attgattcaa atgcttgcaa agccctttta gtgtttaatg tcatttataa
150061 tcattagttc tcctgcctta ctttctatgt acattggaga agctttgttt aatatagctt
150121 actatgactt atgtagatac tgtggaccaa taacccaaaa tagatttgag attggagcta
150181 atttagaatg atgaattaag acaaataatt aaaggttaga aaatgaccaa gaaataatga
150241 gttaggaagg aagaagaaca aagcttaaat tagaaatagK cgcaagtggc aaggtcatta
150301 atcaccggtc tcacgtgtac cttctggggg aggagactcc gctctttctg gaacaggaac
150361 agctggtttc tcttccaaga caggtttctt tggcacttct ggcactttaa agatattaat
150421 tcatttttct tatgagtagt tgagaagtat attaaattta taccacatcg acttcacatt
150481 ttgctcaaat aacccaggga cagatccaag aacaaatttc acaataaggY cagtgatctg
150541 tctttggacc ctcatatagt ggggaggaaa atatggccta ttaagaataa aagaaccaag
150601 ttagtaaact tttccaRtgc ttgtaataag taaatatatc actttttaaa gtcaatgaaa
150661 aaattattaa aaagtattca gaccctaaaa atccagaatg acagttttgt agttgcaaat
150721 ttaagaggac tcgcaaacaa aaggtttgat aataggtgac ttattagaca agggtgagtg
150781 cttttctgca gaatctcatt agtgacatgt accttttgct ggtgggactt ctggcttttt
150841 gggaacagct actttctttt ctggaacaac ttcttttgga acttcaggca cttcaaatat
150901 attagtattt taacattaga aacaatcacc agtaaacatt catcacattt acacagaaca
150961 aaaccctttt agaagctggg ggctccatcc gcccccatca aacagtggac agccacatat
151021 acctttagca ggtggggctt ctggcttttt gggaaccacc agaggcacct tcttttcagg
151081 aacaacctcc ttgggcacct cgggcactat aaaagatatt agtagttgtt taagctcatg
151141 gtttcaatga aatgtgaaaa tcatgaagca gaacagtaga atatgacact tcaaagaaag
151201 ttttttgtta gggagttagt ggcagtgagg aatacctttc actggtggta gttcaggttt
151261 tttggcaacg acagcaggtg ctttcttttc tgggacaggt ttcttaggtg gtacggtcac
151321 taaagaatta gaaggtatgt tttagaaaga acgaagattg agaaacaaga caaaagctca
151381 gagcacccag aattatcagg gcaggaaggg gaaagagtgg ccgaggtgtc ctagcagctt
151441 tcttgccatg taccttgtgg aggcgccgct ggctctggct cttccacaac ttcagcagga
151501 ggctcttcta gggcaacttc ctcaggctcc tcgaacactt taaagacatg agctcatttt
151561 aatgccagaa ttgactaaaa ctgagatagt ttgcaaaaaa atgtttttac aacactaagg
151621 aaagattttt ttaaaaaaca ctaatttgaa tagtttcatt attaccttca gggggaggac
151681 tttccggttt gggaggaata gcttcaggca ccttcttttc tgggacagct acctttggca
151741 cctctgggac tttaaaagat attattattt tcattgttag acaaagtaaa gacaaacaaa
151801 caatatcaaa cacagcacca tgagggtgtc taccttttgt gggtggcact tcaggctttt
151861 taggaggagg cactggcact ttcttttcag gaacaacttc tttgggagcc tcaggcactt
151921 gaaagataat agtgaaatta catttaggca ttatgaagac cactagaaaa atactttcca
151981 gagcagaaga gtttgatcat ctgaagccta aaatcagtga caaatacctt taacaggtgt
152041 gacttcaggc tttttaggag gagccgctgg cactttcttt tcaggaacaa cttctttcgg
152101 agcctctggc acttaaaaga tattagtgaa attacattta gaagttatga agaccattag 152161 gaaaaatatt ttcaagagta gaagagatag atcttctgac gcttaaactc aatgacaaat
152221 acctttaaca ggtgggactt caggcttttt aggaggagcc gagggcactt tcttttcagg
152281 aacaacctct ttgggagcct ctggtactta aaagatatta gtgaaattac atttaggggt
152341 tatgaagacc actagaaaaa atattttcaa gaatagagga gtttgatctt ctgaagccta
152401 aagccagtga caaatacctt taacaggagg gacttcaggc tttttaggag gagccaaggg
152461 cattttcttt tcaggaacaa cctctatggg .agcctcYggc acttaaaaga tattagtgaa
152521 attacattta gaagtttgaa gaccactaga aaagtatttt caagaaatga agagttcagt
152581 cttctgaagc ctaaagccag tgacaaatac ctttaacagg tgggacttca ggttttttag
152641 gaggagtcac tggcactttc ttttcaggaa caacttcttt gggagcctct ggcacttaaa
152701 agatattagg taaaattaca tttaggggtt atgaagacca ctggaacaaa atgtcttcaa
152761 ctgcaaaaga attagatcat ctgaagccta aggtcagtga caaatacctt taacaggtgg
152821 gacttcaggc tttttaggag gagccaaggg cactttcttt tcaaggacaa cttctttggg
152881 agcctctggc acttaaaaga tattagtaaa gttacatgta gagctatgga gactactagc
152941 aaaatataca gcagaggaat tggatcttct gaagcttaag gtcaaatgac aagtacctgt
153001 aacaggtgga acttctggct ttttaggaag caccagtgtt ttcttttctg gcacaatttc
153061 ttgtgggact tcaggcactt gaaagatatt agtagttttt cacttaggtt aatgagacaa
153121 atggagtaaa atatttctaa gatcagaaga gatatttctt ctgcagaaaa aggacagggg
153181 taaaaaatac ctgtggcagg tggggcttct ggttttgtgg gaggagcctt aggaactttc
153241 ttttctggga caacttcttg agcttcaggc acttgaaaga tattagtagt tttagactta
153301 agttaatgaa gagaaatggg ctaaaattgt ttacagtagg agaggagata tctcttctgc
153361 agaatgaagt cagggctaaa gtgtacctgg gacaattgga gcttctggtt ttttgggtgg
153421 agccacggga atttcttttt ctgcggcttc ttgaggaact tctggcactt gaaagatatt
153481 agtagtttta tacttaggtt aatgaagagg aatggactaa aattgttttc aggaatggaa
153541 gagagatttc ttctgcagga taaggttgag ctgacatgta cctgtaactg cgggggcttc
153601 tggttttttg attggtgcct tgggaatttt cttttctggg acaacttctt gagcagcttc
153661 aggcacttga aagatattag tatttttata atttatgaat ggcgaaggta tatattacag
153721 tgattgtgag gggtacagac agtaagttat tcttagcaga ggagagggaa taaatacctt
153781 ttgcacgtgg ggcttccggt tttttgggca cagccacaga tactttcttt tcaagtacaa
153841 cttctttagg agcttcagga actttgaaga tattagtatc ttttagttag aagctataaa
153901 gggggaatat cgactccaca tttacccaag caaatacaac ttgtgagatc ggcggacact
153961 tctatacagt ctttccccag ggccccccga ctgacaatgt gtaatgatac ctacctttag
154021 gaggtggagc ttctggcttt ttggcaggag gcaccggtac tttcttttct gggaccactt
154081 ccttcggtgg cagcacttca ggcacttcaa agatatttgt aatttgtgtt tagaaaaggt
154141 gaaaatgatg gatgcctttt gcatataaac acccaccaag atattttgga tagtgattga
154201 catttgtttt ctttagaatt atatcatctt tatgtagtag gatttttaac atgtaatttc
154261 ctagttaaaa taacagttat ttttctccta tagtttgtat agctttggca ttaccttcag
154321 ggggaggact ttccggtttg ggaggaatag cttcaggcac cttcttttct gggacagctg
154381 cctttggcac ctctgggact ttaaagatat tagtattttc attattagac aaagtaaaga
154441 caaacaaaca atatcaaaca cagcaacaag agggtgtcta ccttttgtgg gtggcacttc
154501 aggcttttta ggaggaggca ctggcacttt cttttcagga acaacttctt tgggagcctc
154561 aggcacttga aagatattag tgaaattaca tttaggcatt atgaagacca ctagaaaaat
154621 attttccagc agcacatcaa aaagcttatc caccatgatc aagtgggctt catccctggg
154681 atggaaggct gattcaacat acaaaaatca ataaacgtaa tccatcatat aaacagaacc
154741 aacgacaaaa accacgtgat tatctcaata gatgcagaaa gggcctttga caaaattcaa
154801 cagcccttca tgctaaagac tctcaataaa ttaggtattg atgggacata tctcaaaata
154861 ataagagcta tttatgacaa acccacagcc agtatcatac tgaatgggca aaaactggaa
154921 gcattccctt tgaaaactgg cacaagacag ggatgccctc tctcaccact cctattcaac
154981 atagtgttgg aagttctggc cagggcaatc aggcaggaga aagaaataaa gggtattcaa
155041 taaggaaaag aggaagtcaa attgtccctg tttgcagatg gcatgattgt atatσtagaa
155101 aaccccatcg tctcagccca aaatctcctt aagctgataa gcaacttcag caaagtctca
155161 ggatacaaaa tcaatgtgca aagatcacaa gcattcttat gcaccaataa cagacaaaca
155221 gagagccaaa. tcgtgagtga cctcccattc acagttgctt caaagagaat aaaataccta
155281 ggaatccaac ttacaaggga tgtgaaggac ctcttcaagg agaactacaa accactgctc
155341 aacgaaataa aagaggacac aaatggaaga acattccatg ctcatggata ggaagaacta
155401 ataccgtgaa aatggccata ctgcccaagg taatttatag attcaatgcc atctccgtca
155461 agctatcaat gactctcttc acagaattgg aaaaaactac tttaaagttc atatggaacc
155521 aaaaaagagc ccacattgcc aagtcaatcc taagccaaaa gaacaaagct ggaggcgtca
155581 cgctactgta ttacaaggct acagtaacca aaacagcatg gtactggtac caaaacagag
155641 atatagacca atggaacaga acagagccct cagaataata ccacacatct gcaaccatct
155701 gatctttgac aaacctgaca aaaacaagaa atggggaaag ggttccctat ttagcaaatg
155761 gtgctgggaa aactggctag ccatatgtag aaagctgaaa ttggatccct tccttacacc
155821 ttacacaaaa attaattcaa gatggagtaa agacttaaat gttagaccta aaaccataaa
155881 aaccctagaa gaaaacctag gcaataccat tcaggacata ggcatgggca aggacttcat
155941 gtctaaaata ccaaaagcaa tggtaacaaa agccaaaatt gacagatagg atcgaattaa
156001 actaaagagc ttctgcacag caaaggtact accatcagaa tgaacaggca acccacagaa
156061 tgggagaaaa tttttgcaat ctactcatct gacgaagggc taatatccag aatctacaat 156121 gaactcaaac aaacgtgtaa gaaaaaaaca accccatcaa caagtgggcg aaggatacaa
156181 acagacactt ctcaaaaaaa gacatttatg cagccaaaat acacaagaaa aaatgctcat
156241 catcactggc catcagagaa atgcaaatca aaaccacaat gagataccat ctcacaccag
156301 ttagaatggt gatcattaaa aagtcaggaa acgacaggcg ctggagagga tgtggagcaa
156361 taggaacact tttacaatgt tgctgggact gtaaactagt tcaaccattg tggaagtcag
156421 tgtggcgatt cctcagggat ctagaactag aaataccatt tgacccagcc atcccattac
156481 tgggtatata cccaaaggat tataaatcat gctgctataa agacacgtgc acacgtatgt
156541 ttattgcggc actattcata ttagcaaaga cttggaacca acccaaatgt ccatcaatga
156601 tagactggat taagaaaatg ttccacatat actccatgga atactatgca gccataaaaa
156661 atgatgagtt catgtccttt gtagggacat ggatgaaact ggaaaccatc attcttggca
156721 aactatcaca aggataaaaa accaaacatt gcatgttctc attcataggt gggaattgaa
156781 caatgagaac acatggatac aggaaggggg acaccacaca ccggagtgtg ttgtgaggtg
156841 gggggatggg ggagggatag cattaggaga catacctaat gtaaatgacg agttagtggg
156901 tgcagcacac caacatggca catgtataca tacgtaacaa cctgcacgtt gtgcacatgt
156961 accctagaac ttaaagtata ataaaaatat atatatatat aaaaagaaaa atattttcca
157021 gagcacaaga gatagatcat ctgaagccta aaatcagtga caaatacctt taacaggtgg
157081 gacttcaggc tttttaggag gagtcgaggg cactttcttt tcaaggacaa cttctttggg
157141 agcctctggc acttaaaaga tattagtaaa gttacatgtg gagctatgga gactactagc
157201 aaaatataca gcagaggaat tggatcttct gaagcttaag gtcaaatgac aagtacctgt
157261 aacaggtgga acttctggct ttttaggaag caccagtgtt ttcttttctg gcacaatttc
157321 ttgtgggact tcaggcactt gaaagatatt agtagttttt cacttaggtt aatgagacaa
157381 atggagtaaa atatttctaa gatcagaaga gatatttctt ctgcagaaaa aggacagggg
157441 taaaaaatac ctgtggcagg tggggcttct ggttttgtgg gaggagcctt aggaactttc
157501 ttttctggga caacttcttg agcttcaggc acttgaaaga tattagtagt tttagactta
157561 agttaatgaa gagaaatggg ctaaaattgt ttacagtagg'agaggagata tctcttctgc
157621 agaatgaagt cagggctaaa gtgtacctgg gacaatcgga gcttctggtt ttttgggtgg
157681 agccacggga atttcttttt ctgcggcttc ttgaggaact tctggcactt gaaagatatt
157741 agtagtttta tacttaggtt aatgaagagg aatggactaa aattgttttc aggaatggaa
157801 gagagatttc ttctgcagga taaggttgag ctgacatgta cctgtaactg cgggggcttc
157861 tggttttttg attggtgcct tgggaatttt cttttctggg acaacttctt gagcagcttc
157921 aggcacttga aagatattag tatttttata atttatgaat ggtgaaggta tatattacag
157981 tgattgtgag gggtacagac gataagtttt tcttagcaga ggagagggaa taaatacctt
158041 ttgcacgtgg ggcttccggt tttttgggca cagccacaga tgctttcttt tcaagtacaa
158101 cttctttagg agcttcagga actttgaaga tattagtatc ttttagttag aagctataaa
158161 gggggaatat cgactccaca tttacccaag caaatacaac ttgtgagatc ggcggacact
158221 tctatacagt ctttccccag ggccccccga ctgacaatgt gtaatgatac ctacctttag
158281 gaggtggagc ttctggcttt ttggcaggag gcaccggtac tttcttttct gggaccactt
158341 ccttcggtgg cagcacttca ggcacttcaa agatatttgt aatttgtgtt tagaaaaggt
158401 gaaaacgatg gatgcctttt gcatataaac acccaccaag atattttgga tagtgattga
158461 catttgtttt ctttagaatt atatcatctt tatgtagtag gatttttaac atgtaatttc
158521 ctagttaaaa taacagttat ttttctccta tagtttgtat agctttggca ttaccttcag
158581 ggggaggact ttccggtttg ggaggaatag cttcaggcac cttcttttct gggacagctg
158641 cctttggcac ctctgggact ttaaagatat tagtattttc attattagac aaagtaaaga
158701 caaacaaaca atatcaaaca cagcaacaag agggtgtcta ccttttgtgg gtggcacttc
158761 aggcttttta ggaggaggca ctggcacttt cttttcagga acaacttctt tgggagcctc
158821 aggcacttga aagatattag tgaaattaca tttaggcatt atgaagacca ctagaaaaat
158881 attttccagc agcacatcaa aaagcttatc caccatgatc aagtgggctt catccctggg
158941 atggaaggct gattcaacat acaaaaatca ataaacgtaa tccatcatat aaacagaacc
159001 aacgacaaaa accacatgat tatctcaata gatgcagaaa gggcctttga caaaattcaa
159061 cagcccttca tgctaaagac tctcaataaa ttaggtattg atgggacgta tctcaaaata
159121 ataagagcta tttatgacaa acccacagcc agtatcatac tgaatgggca aaaactggaa
159181 gcattccctt tgaaaactgg cacaagacag ggatgccctc tctcaccact cctattcaac
159241 atagtgttgg aagttctggc cagggcaatc aggcaggaga aagaaataaa gggtattcaa
159301 taaggaaaag aggaagtcaa attgtccctg tttgcagatg gcatgattgt atatctagaa
159361 aaccccatcg tctcagccca aaatctcctt aagctgataa gcaacttcag caaagtctca
159421 ggatacaaaa tcaatgtgca aagatcacaa gcattcttat gcaccaataa cagacaaaca
159481 gagagccaaa tcgtgagtga cctcccattc acagttgctt caaagagaat aaaataccta
159541 ggaatccaac ttacaaggga tgtgaaggac ctcttcaagg agaactacaa accactgctc
159601 aacgaaataa aagaggacac aaatggaaga acattccatg ctcatggata ggaagaacta
159661 ataccgtgaa aatggccata ctgcccaagg taatttatag attcaatgcc atctccgtca
159721 agctatcaat gactctcttc acagaattgg aaaaaactac tttaaagttc atatggaacc
159781 aaaaaagagc ccacattgcc aagtcaatcc taagccaaaa gaacaaagct ggaggcgtca
159841 cgctactgta ttacaaggct acagtaacca aaacagcatg gtactggtac caaaacagag
159901 atatagacca atggaacaga acagagccct cagaataata ccacacatct gcaaccatct
159961 gatctttgac aaacctgaca aaaacaagaa atggggaaag ggttccctat ttagcaaatg
160021 gtgctgggaa aactggctag ccatatgtag aaagctgaaa ttggatccct tccttacacc 160081 ttacacaaaa attaattcaa gatggagtaa agacttaaat gttagaccta aaaccataaa
160141 aaccctagaa gaaaacctag gcaataccat tcaggacata ggcatgggca aggacttcat
160201 gtctaaaata ccaaaagcaa tggtaacaaa agccaaaatt gacagatagg atcgaattaa
160261 actaaagagc ttctgcacag caaaggaaac taccatcaga atgaacaggc aacccacaga
160321 atgggagaaa atttttgcaa tctactcatc tgacgaaggg ctaatatcca gaatctacaa
160381 tgaactcaaa caaacgtgta agaaaaaaac aaccccatca acaagtgggc gaaggataca
160441 aacagacact tctcaaaaaa agacatttat gcagccaaaa tacacaagaa aaaatgctca
160501 tcatcactgg ccatcagaga aatgcaaatc aaaaccacaa tgagatacca tctcacacca
160561 gttagaatgg tgatcattaa aaagtcagga aacgacaggc gctggagagg atgtggagca
160621 ataggaacac ttttacaatg ttgctgggac tgtaaactag ttcaaccatt gtggaagtca
160681 gtgtggcgat tcctcaggga tctagaacta gaaataccat ttgacccagc catcccatta
160741 ctgggtatat acccaaagga ttataaatca tgctgctata aagacacgtg cacacgtatg
160801 tttattgcgg cactattcat attagcaaag acttggaacc aacccaaatg tccatcaatg
160861 atagactgga ttaagaaaat gttccacata tactccatgg aatactatgc agccataaaa
160921 aatgatgagt tcatgtcctt tgtagggaca tggatgaaac tggaaaccat cattcttggc
160981 aaactatcac aaggataaaa aaccaaacat tgcatgttct cattcatagg tgggaattga
161041 acaatgagaa cacatggata caggaagggg gacaccacac accggagtgt gttgtgaggt
161101 ggggggatgg gggagggata gcattaggag acatacctaa tgtaaatgac gagttagtgg
161161 gtgcagcaca ccaacatggc acatgtatac atacgtaaca acctgcacgt tgtgcacatg
161221 taccctagaa cttaaagtat aataaaaata tatatatata taaaaagaaa aatattttcc
161281 agagcacaag agatagatca tctgaagcct aaaatcagtg acaaatacct ttaacaggtg
161341 ggacttcagg ctttttagga ggagccgagg gcactttctt ttcaaggaca acttctttgg
161401 gagcctctgg cacttaaaag atattagtaa agttacatgt ggagctatgg agactactag
161461 caaaatatac agcagaggaa ttggatcttc tgaagcttaa ggtcaaatga caagtacctg
161521 taacaggtgg aacttctggc tttttaggaa gcaccagtgt tttcttttct ggcacaattt
161581 cttgtgggac ttcaggcact tgaaagatat tagtagtttt tcacttaggt taatgagaca
161641 aatggagtaa aatatttcta agatcagaag agatatttct tctgcagaaa aaggacaggg
161701 gtaaaaaata cctgtggcag gtggggcttc tggttttgtg ggaggagcct taggaacttt
161761 cttttctggg acaacttctt gagcttcagg cacttgaaag atattagtag ttttagactt
161821 aagttaatga agagaaatgg gctaaaattg tttacagtag gagaggagat atctcttctg
161881 cagaatgaag tcagggctaa agtgtacctg ggacaattgg agcttctggt tttttgggtg
161941 gagccacggg aatttctttt tctgtggctt cttgaggaac ttctggcact tgaaagatat
162001 tagtagtttt atacttaggt taatgaagag gaatggacta aaattgtttt caggaatgga
162061 agagagattt cttctgcagg ataaggttga gctgacatgt acctgtaact gcgggggctt
162121 ctggtttttt gattggtgcc ttgggaattt tcttttctgg gacaacttct tgagcagctt
162181 caggcacttg aaagatatta gtatttttat aatttatgaa tggtgaaggt atatattaca
162241 gtgattgtga ggggtacaga cgataagttt ttcttagcag aggagaggga ataaatacct
162301 tttgcacgtg gggcttccgg ttttttgggc acagccacag atgctttctt ttcaagtaca
162361 acttctttag gagcttcagg aactttgaag atattagtat cttttagtta gaagctataa
162421 agggggaata tcgactccac atttacccaa gcaaatacaa cttgtgagat cggcggacac
162481 ttctatgcag tctttcccca gggccccccg actgacaatg tgtaatgata cctaccttta
162541 ggaggtggag cttctggctt tttggcagga ggcaccggta ctttcttttc tgggaccact
162601 tccttcggtg gcagcacttc aggcacttca aagatatttg taatttgtgt ttagaaaagg
162661 tgaaaatgat ggatgccttt tgcatataaa cacccaccaa gatattttgg atagcgattg
162721 acatttgttt tctttagaat tatatcatct ttatgtagta ggatttttaa catgtaattt
162781 cctagttaaa ataacagtta tttttctcct atagtttgta tagctttggc attaccttca
162841 gggggaggac tttccggttt gggaggaata gcttcaggca acttcttttc tgggacagct
162901 gcctttggca cctctgggac tttaaagata ttagtatttt cattattaga caaagtaaag
162961 acaaacaaac aatatcaaac acagcaacaa gagggtgtct accttttgtg ggtggcactt
163021 caggcttttt aggaggaggc actggcactt tcttttcagg aacaacttct ttgggagcct
163081 caggcacttg aaagatatta gtgaaattac atttaggcat tatgaagacc actagaaaaa
163141 tattttccag agcagaagag atacatcatc tgaagcctaa aatcagtgac aaataccttt
163201 aacaggtggg acttcaggct Ytttaggagg agccactggc gctttctttt caggaactac
163261 ttctttggga ggctctggta cttaaaagat attagcaaaa ttacattcag aagttatgaa
163321 gaccactgga aaaaatatct tcaagagcaa aagaatgaga tctgaagcct aaggtcagtg
163381 gcaactacct ttaacaggtg ggacttcagg ctctttagga ggagccaagg gcactttctc
163441 ttcgcggata acctctttgg aagcttctgg cacttgaaag atattagtga aattacattt
163501 aggtgttatg•aagaccgcta gaaaaaaata ttgtcaagag cagaagaatt aggtcttctg
163561 aagcctaaag tcagtgacaa atacctttaa caggtgggac ttcaggcttt ttaggaggca
163621 ccaccgacac Yttcttttca gggataatct ctttgggagc ttcgtgcact tgaaagatat
163681 tagtattttt acactcagaa aatacagaga ttactagata gatacaaaga cattttcaag
163741 aacaaaagag ctattttttt ctcctgagct gagatcagtg gtaacaagtt cccataatag
163801 gtggtgtttc aggttttaga ggaggaactt cYggtatttt ctttttggta gccatttctt
163861 tgggagcttc aggcagtttg aagatattaa tacatttaca cttgagttgc aagagtcaac
163921 acagacatga ttcacatgta cacatcaaaa taattttcaa aactagaaag gtggtccttt
163981 ctatcgcccc acccactatc ccaccataaa aagacagtta agaatgtacc tttgacaggt 164041 acaacttcag ccctttgggg aggatgcact ttcttttccg ggacaacttc tctgagagcc
164101 tccggcactt tgaagRtatt aataatttta catttagaag ttacaagaat caacacaatc
164161 aggaaaaaca tttatacaag aaaagacatg ttcccacccc tctaagcttc cagcaagata
164221 taccttcatc aggaaggact tcaggctttc tgagaggaac cacaagcgtt ttcttttcag
164281 ggacaatttc ttggagagct tcaggcactt taagaaatta gtagttttat gtttagtgtK
164341 atgcagataa ctaggaatag caaaaatatt ctcaaaacta caagagctag ttcttgaccc
164401 tgagagcatg gtgacttgta cctttaactg atgggggttc tctttttttg gaaggaactg
164461 tcttggcagg aataatttct tgaggagctt cgggcgcttg aaagatatta gcaattcaca
164521 tttaagagtt aaaatgatta atggaaaaca gtaaccacaa taagttagga aatataacca
164581 cattttcttc tctttcctcc atcctccctt tgttccacca ataggctaag tcttttaagg
164641 ggttagtgga tccttgagag gaggtgctcc ccctcctcca gaacctcaca gagctctcgc
164701 gacactctac actctcatta taaaggattt gttgaatgaa tacttccagc aggagtgttt
164761 tctgttttgg cagaaacagc cataaataat ttctttttag aggaaacttc ctgtggaacc
164821 tcagacactt aaaagatatt agtatgttta tatttagaat ttatgaagag tattaggaag
164881 aaaccatagt tttaagagca gaggacagat aatttcttct tccacatttg ttcagaggta
164941 acgtactttt catacgtgga gtttctggct cttcaggtac atcctcaaat attttcattt
165001 cagggacaac ttctttcata gcttctggtg ctttgaagat attagtatta tggttagagg
165061 ttaaaaggat cagtggagac attaattaaa tgagctgaac caaagtgata tttatggcta
165121 aaaagatgat tcctttaaag aatctgagga ggtatacaat cttgaggaga ggaaccacat
165181 attaattgtc tttgtttatt attctccagt tccctagcac cctgtacatg ctaagcactc
165241 taataaatga aggaaggaat ggcaggatga acgatacctt tagtgggagg tatttcaatt
165301 tcaaggggag cagccatggg tgtttccttt acagggacaa tttctcgagg tgattcaggc
165361 actttaaaga tatgaatgca ttgtactttg gagttatgaa gaggagtaaa gagttcccat
165421 cttaactgca catacagaac attccagatg ggaaccttct cccactcccc gcccccagaa
165481 tcggatcttt tgtagcagtg tgagtatttg gttataagcg gctgttttct tggtcacctc
165541 aggcactatc agtattagta attttatttc ttggaatggt agatgcatag aaaactagat
165601 catggaaagt ggaaacattg attataatta atgctatacc tctagctatt atgttgagaa
165661 aaacaaatta cagccacttc gaaaaatcta ggtcggcatt tttttgtatt cacacctcag
165721 gcaccttaaa aaggtgagtg tttttcaagt ttaggagaga ggagaacaac caaaagattc
165781 cagactgaga aaaaatatca aaatattcag atattcttca aattacagaa taccatgggt
165841 gttatgatga aattattgtt ctggaggttt taaagctttt tcatgttttg acttatctaa
165901 agatattcaa aatgaaaaaa aatgatgctt gaaaaataaa agataggcta tgagaggagc
165961 aaatgaacat ggaggggaga tttccagctt taactggaat tatttttccc taggttggag
166021 gatattctgg gagggtctca ggtgctctaa tcttgtagag atattacaga tcagcttcac
166081 actgaattaa tattttcttt aagacatgtt ttgatcataa tatgtgtata tatattttct
166141 ttggagtcct cataaaaata cactgactca actatctttc atgtgtataa gctaaaggta
166201 ttttttccct ctgttgtctc ttgagtgact cactgggaca tgaatacatt tggttgagct
166261 tctacttggg ggatccatct ctgaaatgat ttccactgta gccactttgg cttaaaagat
166321 attagtattt ttacatgtgt taagtacaac tgcaaacatg agattaaaaa ggtgactttc
166381 ttccaacttg tacctgttgg tgatggtgtt tttcttcttt taacaatagg agtttctccc
166441 tctggaatga cttccttgaa gacttcaaac tctttaaaga tattagtatt ttttttaatt
166501 gagaaaaggc aaaggcataa agacatgaca tactaRgaaa gttagatatg ttaatatcat
166561 ttcagatatc tttatgttag aaatgttatt tttgttctaa tattttatct ttgtatatta
166621 tatgtgtggg acttgacttt cttgggggca agtoataggt cattctttgt tgtgggtata
166681 tcagatcctt taaacacagt attttaacat tagaggttgt gagaatgtat attaaacatt
166741 aaatattttg cagactgaag acagtatatt ttcttcttta gattttccta actagagaat
166801 tataccttca gttggaggat gttctggaat ttcaggaata gcctcaggtg gctccacctc
166861 tggaaaaatg cctctggttg tatcaggttc tttaaagata ttagtaggtt tacatttaaa
166921 agttgtaaaa acagtaaatt ataaaaagca tgcaatgttt gagtcataaa gctaaagata
166981 ctcatctggg tttgatgtat ttgaaatata cctttagttg ctggtgtttc tctcttttta
167041 ggaatagtca catatatttt gtcttctgga acaactttct tgggtggctc aggcacttaa
167101 aagatatgag tatagttata tttataaatg gtgaagaaaa atattgagct ttttaaaagg
167161 ggccaaaaaa taatttttct tcagagtgga tcattggtgt tatatacctt ttgctagttt
167221 gggttttgtc ttttgaggtt gagtcacaag tactttttct tctaggactg cttcttcaga
167281 tgcttcataa actttaaaga tattagtatt taaataatta ggatgtttca aggtggataa
167341 agaagtgtat taagaaaaat ataatatata ataccacatt catcaccaaa attaatagta
167401 gcacatagag gcaaattagt ggctagaatg cattggctgg ggatagcctc actcccagtt
167461 tcatgatgta agactctaag catttctcta tgatgatgaY aataaaatgg aggaattgtt
167521 caggcaagga ctatataatt cttcattttg ccttaagaaa gttattggta aaattgtcct
167581 attggtctta tttttaatgg atttgtctat aaagaacagg gaagatgctg aattgcacat
167641 tgagaattct ccgatatttg acctactaga aattcatgta agtcttttcc ctttctagaa
167701 gcctcattat ctatattttc aaatcacatc taatatatgg tttcacagtt taatatgaat
167761 gtaaaagcca tttagggttt ggaggcagaa aattaaaaaa attaaatact tagaaaataa
167821 ggcaaataaa gacaacaaaa atttctccag cctttagaaa gaaaaactgt ctgaaaacaa
167881 taactaaagt caagaataga aaggMtattc aaaggaggaa gtgattatat tattttatcc
167941 caacttagta taacaggatg aacaacaaga aagttgagtt tcaacaggaa attttttaga 168001 ttaaaaaaaa tggaWgcggg tctggtgtag tggctcatgc ctgtaatccc agcactttgg
168061 gaggctgagg tgggcgggtc acctgaggtc aggagtttga gaccagcctg accaacatgg
168121 taaaaccctg tctctactaa aaatacacaa aattagccgg gcctggtggt aggcacctgt
168181 aatcccagcc actcgggagg ctggggcagg agaatagctt gaacccagga gacggaggtt
168241 gcagtgagcc aagatcgtgc cactgcactg cagcctgggt gcagcagagt gagattccat
168301 ctcaaaaaaa aaaaaaaaag gaagaatatt ttgactactg tcacttttac tattcaggtt
168361 gttgaacagt attctattca caatatttta ctatttggat tatttcataa ttcaaattat
168421 ttaaacctaa ttttgtatga aacccccccc caaaaaaaaa ggtcgtgaga 'attcttttga
168481 ggtttgagtg gccttgtaaa cctagggtcc tttgagcagt tttgccaaaa tgtttccaat
168541 tatggttatc aagagtaaaa aaaaaaataa tggtgaaaca ttttgatttt ataaaatcca
168601 attaaaatat tatggagtta agttacttca tgatctgaga aaataaattg cttcaaagca
168661 aaagacactt gtatacagaa taggttagga aaatttaaca agacaactga cttttctgaa
168721 atcatacact ctgataagta gagtatttct tttttaaatt ggtgaggaaa tataacttgt
168781 ctgaggataa tagacactaa gattatttga gtcatatatt tgtggcatgt taggctttta
168841 taagagttta gtatatttac ttttcaaagc taaaaagaca aacatagtga atttaaggac
168901 ataaatgaaa cgaaaaagaa ccactaattt ttctacactc actgtacatc tctgtgtctt
168961 cagaaataac aataggtgat ttttcttctt gaacactttt cttaggcatc ccaggaactt
169021 taaagatatt agtatattaa ttgttacaga taacaaatat aagatacgaa atacaaggat
169081 ttaatgtcac acacattcac tttaaaccat gaaaaactaa accaagaatt atttcatgtt
169141 ctgattagca tcactgtata cctttagctg gtggaacttc aggctttttc agaacagctt
169201 cacgaacttt ttcttctggg acaattttct tgggtacttc gggtgcttta aagatattta
169261 tttatcttat tttttcagaa cattatagtt gggtgtaaac atagcaagcc tagaaggggc
169321 atctaacaag atgaacgcca aaaacccctc aattataagt caactataag tcaaatgtag
169381 tcattgcatt tctctgaatt gctttgtcga tgagttcttg attcattata tcagaaacac
169441 tttgctcttt ggagatagta tcattttcta ttgccattta gagcacactt ttttttttcc
169501 agagctactt cagaagagct tccaaaatga attatttttg aaagagaagt tgagggatca
169561 atattattta gttattaaat aagaggatca atacaaatga taactttggg cttatgtctt
169621 tacgcctaaa acattgcctc aaaaggcagg attatgttat agaacataaa ttgaagttta
169681 tgtcattcat agccatcttg tggcattgag aagagaaagg tctctcttac atagtaagtg
169741 aataaaaaag gattttatat taatgatgaa tgagaaaagc cagttacctt tagttggtgg
169801 aactctgggc tcttcaggaa cacgtacttt ttcttctacc acaattttct taggcacctc
169861 cggtacttta aagataatag taataatttc ttttattttt aaatatacaa ttttttaaca
169921 agcagagcat gagagatata aacacagaac agaacacagc aacaatataa aatgcagaca
169981 acactttatc aaatacatta cagtatttca aatgcattga atttaaaaaa tatataataa
170041 caaaaatcca gggaaatttc ccatgttaga atggtaagaa cttgagaaga aaaattaaca
170101 gtacaccttc agctggtgct attactgttt gtttttgtgg aagagttgct accttctctt
170161 cagtgataac ttacgcacat gcttcagaga ctttaaagaa ataagtttat attatttatt
170221 agatactgtt tttatttcta ttgtgaaatt taagagattt gcaaaaatga acaaagcaag
170281 aacattaaac acatattcac tatgtattta tatttgccag gttaatataa ttaataataa
170341 atagaaaaac atgtgtttat cagtaagcat gttagtgaat atataaccaa atagcaccaa
170401 aggaggaaat ttgggctcac tatttggtta ctagagatat ttgctttggt tgttttaggt
170461 ataacaacag tgactgtctt tttttggtag aacttccctt ggaccctcag ctgctttaaa
170521 gatattagtt tgttttagac atgtcagaaa caagaaatac agaagaaaga catcaagtgg
170581 aatgcaaact tgtgcttata aagcaaccaa ggtgctattg tatgtaaagt taagtagtaa
170641 tttttcacaa agaagatacc tttagctggt ggctcttttc gaggaacaac tttagtgggc
170701 ggtttttttg gagggatgat tttctcagat atctcaggcc cttcaaagat attagtattt
170761 tggtttagaa tgaactcttg aagtattttg gagcactact actaacgtta gtacaatgag
170821 gggtcacaaa attctttatc tatatttttt taatttataa cacacttatt tccaaaagag
170881 atttgatttg ctaaatatca tgtatattgt aatttgctat atatgctaaa cacacacaca
170941 cacacattcc ttcatattac acatatattg ttaatttgtg caagatctag agtggtattg
171001 cagataactt atttgtgcta tggcttgtct tcacagtatg ctagaataca ggtgccatta
171061 acaatacttc acttccttaa tgttgtttct ctaatgaaag tatcataaac tggcaaaagc
171121 ttttggtaag tatttctcag tttgtgaaca gttgactaaa atgttaaagc agtgattatt
171181 ttgcctgaac atgtaaa'ttc ctcagtgaac tgaggagttt ctttttcact taaccaatga
171241 cactggtcag ttattttggt acctgtaaca attgtgtaac attcttagtc agatttaaaa
171301 aataatcccc aaattcatac agtgacctcc ttagataagt agatatcaaa tattactatc
171361 atctactaaa attatactcc gtgtggttaa gagatattaa tctttgtgtc aactagttta
171421 aactcatgtt taaagtattt catggggttt ctaatcttcc aaactgaaca caaaatttac
171481 tttcaaagga aagttagagc aagatataag aatttgtagt atttgaagaa ttccctatac
171541 ctttaggtgg agcttttggt ttttcaaata cttccacttc ttcagcctca aaaacttcta
171601 ttaccctatg aactttttca actctgtgtt cttcttcaac tctatgttgt tctaatttga
171661 tgaattcttc tacttcatga aactcgcctt cttcaaaata ttcttcaact tcatggaact
171721 cttcttcttc cggaatttct tccacttctg cttctactac ttctaattct agtctttctt
171781 ttactactac ttcttggcgg aaggcaactg atactttttc ttcaaggaca gttctccctg
171841 aaagagcatc tattttaaga cttatttttt taaacactga agaaataaag atgtacattc
171901 aaaatatata tttttaaaac tgaataaagg attgcatgca acaatacacg aaaatccagg 171961 atctttccaa aaataccttt agctggggga acagcttcct ttttaggcac aaggactttc
172021 ttttctggga ctttctttgg tacttcaggc actttaaaga tacagtttta atatttagga
172081 ctgtcgagag caagctttca tagacaaaaa tcatcaaaaa caatcaagac acagagacat
172141 gaaacataaa agtcttaacg aaaaaagaca gaagaggaag tcaggtttaa aagagaagat
172201 gtacctttgg ctgggggtgc ctcttttttc tgaacaggaa caggtacttt ttcctcagga
172261 attttctttg acactttaaa gatattaggt gttttagtta gctgagaatg ttcaataaca
172321 cacatgaaat aaaaacacca gagacaacgc caacattaca ataattaaag tttttcagaa
172381 gtcactctgt actgtgggac atccatttta gaggcaataa agatactttc tcaaatcact
172441 catttgttct aacattccta tagaagaaga aatgtccaca aagtacctgt gccattcttt
172501 tttttttctt ttaataacag ggattaccaa tatggactca cttttactat ttttaacttg
172561 atgaagactt ttttgaagtg attaccattg tagagtattt aagccctgac actggcctca
172621 ctttctgctg tgcactcttc tattagctga ggagtctact atgacaggga cagcataatc
172681 taactgctta agagcactgt ctgaagcatt aagtgaatta ggaagcaggg aaaagtagac
172741 attttgtaat aaccttgaat gcttgggcta aacattacaa aagggacgtg aggctcttta
172801 aaatagttat tatccaagca ttaggtttgt tccataaaat aataaatata gtaaaataat
172861 tattataaca cagaagaaaa ctccgttcca cttatggaat aaaatctaaa atgtggggaa
172921 gagggatcca ttgctatgtg tataagtata tagagacata ctaatatatt aaaattctca
172981 gttaagaagc ttctagaata attcatacgt aaacacatgc acacaattat tcgttaacat
173041 cctagaaaag attaaagaaa tggaatgagt tatattttta ctggtcctta atcagttcac
173101 tatctaaatg attataagaa ggcagtcaaa aaagaaaatg ttgcttttaa gaaagaaaat
173161 taatgggaag ttaaagatat taataatgag gatttgatat acctttagct ggtggtgcct
173221 ccactttttt aggaacagga gtaggtgctt caggtactgc tttcttaatc acttcaggca
173281 cttaaaagaa attttatgaa cattttgaaa agtggcatgt gatgagcaga acaccaccca
173341 tataccaata aataaatatc acaaaacatt gacttatttt taaaagatat tagcaaccta
173401 aatggtaata ataaatgtgg agtaggaacc ctagaggtgt aaaggatatt acttagaaat
173461 cctataagca aatgtaatac caattatgca aaatattatc acactcttca ggaatagtaa
173521 attaaagatt tttcttaaaa aatgaacact ttcaccagag aaatagattc cccccatatt
173581 aatattaaaa acaaaattaa ggattgaaat ctaggagaaa ctattatagt agggcctggt
173641 cagaaaaaaa ttcaatcgcc aataacaaaa tagtaatctc tcattcttag ttgtattgca
173701 gatgttgtag aaaaatttag ggtaaaaaaa ggacacatag attcaggtgc caggaataat
173761 taatcttcac tttatcatgg atacgatata agaaaagcat actggtgaat cttagattta
173821 gctactgaga aagatttgga acaccaggaa ttttaaatgt tcaatcctta aaagcggtta
173881 tacctctagg tggtgccacc tcttcaactt cctctatgct aggtggttct tctgggattt
173941 cttcttctga aataggctct tcttcaggct cctcagtcac tttaaaaaga ttattatttt
174001 gtaaattatt tttataatta ttttgaatat tagactacag tgataatatc tgaaaactgc
174061 tcagccttcg aaacgcttgt gtaaactcac tagtccttaa aaaatctcta ctgggtcttc
174121 aaaatatgtt aggcatatcc cagcatttgt gaactggaaa ggacttcaga taatttataa
174181 taataaaaaa attattattg tttttttttt aatgaaagac ttgtttgaag tcaaacagcc
174241 acttggggta gaaatcagat tttctgcacc agttactcag gcttaactgg aatttctctt
174301 ggataagctt attttacagt tgcctactaa gtcatttgat gaaaaatttc ccacagcaaa
174361 tacagtttcc cttcatgaat tctaattaaa ataaaacaaa tagcaaacag acaaacaggc
174421 tgaacatgca aagagattca aataatatta atttgcctgc ccaatttcta ttaggttaat
174481 aataatttat tttagaatct gaatatgttg atttcctggg gtaaatttta catttaccta
174541 cttcaaatat tagacaataa taagacaagg atgactttga aatgttattt tcttagaaag
174601 ttatctacct tcaactggta gaacttcctc ttctttaggg agaatgattc gtttttcttc
174661 caccttctta ggcacctcag gaacttgaga gacattgatt attttagaca cttaaaatgc
174721 aagaaccaaa gaagaataaa gaccaMgttt ccagtttcca acataagaga gtaaatatca
174781 caaggcacat acacaagatg aaacattgac atttcacaga atcggttaat gagaaaagaa
174841 taaaaaggtt tgtggtttaa gatttagggt ttattaaaga gaaattctac tttttactgc
174901 tggagcctct atttcttttt tcttttgtct tttttttttt tgtaggaaga aatgggtatg
174961 aagaaatggg tatttcaggg actagttcct tagttatttt tggctcttta aagatatatt
175021 tttatttttt agaaacaaca tacatagaga aacgatgtaa agcacaccaa caccaagtga
175081 aaaaatggac acaacacaaa cttgggtgca aaagagtcag aaatgacgaa tgtgaatgac
175141 agaccataca atgcacacat ttatgttgag tgtcctgtgt ggatagaacc acagttcaac
175201 atcggtgcag caaacatatc cctgttatgg gatgatgtac ctttggcagg aggggccact
175261 gctttcttaa gaccaggagg agggaccttc ttttctggct caggtttctt aggtaccaca
175321 gacactttaa aaatattatt ttattttata agttcagttt cacacacaca aagacaagta
175381 gacacagcag taatataagt tgtattaaca aatacggaaa caggacattt tgacttttat
175441 aggagaagga aggaaatggc atagtctaat ttacttcgga atagcaatac ctttggcagg
175501 gggagcctcc tctttcttgg gaatgaccac tttcttctct gtcactttct tcttaatttc
175561 aggcacttta aagacatcat ttcatgataa ggatttattt tgaaggacaa aaaaagagaa
175621 accaagaaga ccccacaccc agaaagacca atgtgttaac agtcgctcaa gacaggtctg
175681 tcatgttagt tgggagccca gatattataa tgtaattggg atttgtaaag caagggacca
175741 gcagcatatg tcattatcag aggacagatg ctattagggt ggtgcaaaaa taattgcggt
175801 tttgacatta cttttaatgc taaaaaccgc aattactttt gcaccaacct aatatcattt
175861 catacattta aatttcacta gtaatttatt gtctagaaag tatttctgaa tcgttgatat 175921 ccaataaaag atcagcttta ctatttcatt tgattatata actatatggc ctgatgcgag 175981 ggttatgctg attttaagca gccatgccag catcttttgt agaaaatatt attgagcttt 176041 aaagcacaaa tatattaatt ctgtcctgta ctatgagctg attgaatatg ttaaatctgg 176101 atactattca ttcaatgata gactacttta ataccttact ttatttaatt ctcaccataa 176161 tagctgagcc cattaagact gatattagca tccccaattt acaggaaaat aacttcaggc 176221 tcaggttggt taaaggactc gctgagagta atacaacaag gtgtaaagtc aacgctaaaa 176281 tcaaaacctg ttgggctgcc ctttatatat atgtattaga agacataact attattctgt 176341 gttttgataa atacattcat ctttttttct ttttagcctg gtgttaaaaa ggcatttaat 176401 ataaatggtt gaaggaagaa atttaagaat atttattcat aatagttttc aatacattta 176461 aatatatagt atacttattc atatatattt aaatatatct ccatgaaaac tatgatgttt 176521 aatcaaatga tatgaaatca gaacttttgt atcagttctg ggacaatgct tcctttcatc 176581 tttgttcttg aatactgctt tgtatcagtg aaaaaagtag atatcagtgt tcctgaacat 176641 cgttagaagt aaatatgtaa ctgtgtgatt atccattttg taaatataat tttcctaaaa 176701 cccagtttca tcataattta tgtgaccaag ctatgggtga aSaatgtgta ttcactttgg 176761 ggaggtgtac ctttgggtgg tggtggaggt tccacttttt taggggcggg aacagggact 176821 ttcttctctg gtacaggttt ctttggcact tcaggcactt aaaaacattt catttgaagg 176881 cattaaaaac cacatataca tggtcatata tatatatata tatatatata tgaaagagac 176941 acgtgaggat aagaccacaa acatgtaaac ataatatgtg atttgaaaag acatttagat 177001 Ratttacaat agacagatac acaaggcaga tacacaataa gaacacacat gtctggaatg 177061 ttttctaaga tttagRtggt caatcacaga gggtaaagga ttatagataa aactggagat 177121 gaacaaaagg atgggaaagc agaattcaac acaaaagagg ccttgatgat tccaagaaga 177181 gctgctatac ctggtgcagg tactggcacc ttaggtttaa cttctggaag gacttcttct 177241 tcaggtacaa attcttcttc ctcaggtaca tattcttctt cgggaggaac ttcctcttcc 177301 tcaggtggaa tttcctcttc ttcaggtaga acttcctctt cttcaggtag aacttcctct 177361 tcctcaggta gaacttcctc ttcctcaggt agaacttc'ct cttcaggaac aatttcttct 177421 tcaaatagaa cttcctcttc ctgaggtaga gctacaggaa ctggaactgg ttcacgtttc 177481 tttggcactt taaagatatt tattcaaggg tacaaacatc acttttatgt aaaatattca 177541 caacaatgaa gaagcttatg aaaggcaaat attctgtgat cacaaagcat caattattat 177601 caacataaat actaagcatt aattataatt agtaaggctg tataacacca gttagttttg 177661 tcttttacca tggctctgtt acagattaat gtacctttgg gtggtggtgc ttccactttt 177721 ttcggaacag gtgttggttt cttttcttct gggatgagct tcttgggcac ctctggcact 177781 ttaaagatat tatttatatt taggaatatg ttcttttaaa atgtcttaga gcaatagata 177841 tgaaaaagaa tcacaaaata tctgacatat ttctaaccag atagacactt taaaaatgta 177901 caatgtaatg gggaaatttg tatgtgagta tacatagatg tgagtttttt cccccaagta 177961 ctctaagtga tgaaattatg taccttttgc aggtggagcc tccactttct taggagcagg 178021 aactggcacc ttcttctcag gcacaggctt cttgggtacc tctggcactt taacgaaatg 178081 atttagagaa aaattttata cgacataaaa atactgattc caagcatagt ttcaaaagat 178141 taagataagg taataaaagt atgacaaagg tattccaaga agcattttat ttttaaaata 178201 aaatatttaa ttttctctaa agagttgcat cccaaagagt acagaattaa accaattaat 178261 ttttacccat atgcaaatgt gaaataaaaa tattgcaaca ttgcaagttc atgtaccttt 178321 ggcaggtgga gcttccacct ttttaggaac tggtactggt actttctcct ctggcacagg 178381 tttcttgggc acttcaggaa cttcaaagat atcaaataga gttagtgtca cattttttac 178441 ccatagctaa gattttaagg ctggcaatgt aaggccttaa ctgaagcagc atagatattc 178501 tatcaatttc actttaagac tgaaaattat aatagccaca aaacaaaggc cagaaaatac 178561 acaaaaatta aaataaaaaa caccgaaaaa aacgtggact ggtgataaag acaagacaca 178621 atgcctggta aatgatggtt ttgtccattt tgatatgcaa aaggaatgta ttttacttga 178681 agaactttcc actaaagagg aggagccaac cattgagcat aagcataatt tgatggtaaa 178741 attcagagat gaataccggg taaactgctc ctgtggccag ttgagaggcg cttgtcatgg 178801 cattaatgtt gttaatattg tcatggggaa agatctgcta tggctcacca agttatgctg 178861 catgggtgaa ttatcatgct attccactga tggattataa ggatgtacct tttgctggcg 178921 gaggcttctc ctttttagga ataagcacag gaactttctc ctctggcttc ttaggaacct 178981 caggcacttt aaagatattg tttattgtta agttctaact actagtaaca acacatccat 179041 ataaaacaca gctaatgttt tttaacaacg gacagtgaca cacacacaag gttttgaact 179101 cagaagaaag ttaactattt ctaggcagat gaagtctctt agatacccgt caatgaatgg 179161 tggtgtacct tttgccggtg gagcttcctt cttcttggga acaggaacag gtttcttctc 179221 ttctggaaca ggtttcctgg gtacctcagg cactttaaag atattattaa gaatgttgga 179281 aattttgcag gaaagaaata aaatgtgaaa agcacctgta gaaatcatat ttcagcatct 179341 aaaaatatct gctttaaagt aagcaatcat tggtgctgcc aataaccccc agaatctgac 179401 caaatcagac ccccaaactc caaagatgct gttgcacagc cctttgcagg aggcatcatc 179461 tacctttgac tggtatcact ggcaccactt cttcctcagt tatgaactcc tcttcttcat 179521 gaatgtactc ttcttcttct tctacaagat attcttctac atgggttaca acttcctctt 179581 caaaggcaac ctcttctggt tcaagttctt taggcacttc tggcacttta aagatattat 179641 ctttaagttg gacatttgcc aatgactcaa caaaattaag acaccttaga aacacaggcc 179701 catttataac agaagaaagg acaagattta cttttcccac aaaagaagtt ttcacacaca 179761 gaactgaaga ggaattcaac agcaaaagac gaacaaaaca ttaaaatgag tgccattagg 179821 tacctctaac aggtggtgct acttcttttc tagggacagg tactttttct tctgcgacaa 179881 ccctcttggg cttcatgggc acttgaaata tgaagtataa ggaaatttta ttgtcagtaa 179941 aaggccaccc agccaatgct tgttttgctg tactttaagc agatagaata atgtatcttc 180001 ctttaatgag atacatatgc gattgttcat ctttaagaaa ctatatactc tctgtggaaa 180061 ggattctgag cttcatataa atacaagtac acagaagtac ccaagtaaac atgaagtagc 180121 agagcccaaa tgtgaaatga ggtttagtgt tcaaatatct ctgcttgaaa gaaatatcct 180181 tcctgaaaag tgaaatttac ttctcaatta gtatacttca tacttagtat gaatcagggg 180241 gaaaatgact ggaaactggt tagctttaaa aaaaatcttc aaaatgaatt tagaatgaat 180301 aaacagttac caaagcttgg tgaatattat ggtatcatta catcatttag gtaaatcagg 180361 aatatgccaa acggtcactg attttaaacc caaacattct attttatatg tattgtagcc 180421 atgtggcagg acacagccac tggctaacag gctatggatg tatttagaaa taatttttcc 180481 ttttaaaggc attatcttct tatcctgtat ttacacattt ttacatccaa cattctgctg 180541 acaactaatt taaaagacat gaaacaacaa aatgaaatga atcatagtta cattaatttc 180601 aaaagaggca tcagaaaaga tcacaatcga ggaaggaaat cattatacct ttagcagcgg 180661 gttcagtcac ctgctctttt tcacgtttgg taattgaaat acgtattttt tcctcaaaaa 180721 ctttctttgg ttcttcaggc actttaaaga tattaattat taaagagcat taaaaccaac 180781 tccttgaata ctatgtaata aatacacata aaaaactgaa atgtaagaat caggacaaga 180841 acatcctact cagcaaaaac acagaccatc agacgggcta ggaagacatg attttagaac 180901 atacataaca aagttgtttt tggtgattat tactcagtaa tgtacctttg ggtggtggag 180961 gtttgagttt cttaggaatg ggcactggta ctttttcttc agggacagct ttcttcagca 181021 cttcaaaata tcaatattaa gagattttaa aaattgaaac aggtttctca aggctagcaa 181081 gaacaaaaag ttaagaaaga caaatacaac ataatattcc caaacacaca cataatttgt 181141 acactgccac ttttacatat atttttatag taggacagac atggaggaaa caaagatctc 181201 ttacaggtaa taatgttttt ttcactctac ctttagccgg tggggccttt ggttttgtgg 181261 gaactggttc ttctgggaca ggctttacag ggataggctt ctctggttct ttaaaagtac 181321 atacaaggta tttcatgtta gacttagaat ataagtttaa acatttctta aaaggaatca 181381 aataattgaa ataatatctt ttatctacag tatagccata gcctattgaa gtaggcaaga 181441 atcagagatc caagcagaac aagctcatat tgccctttcc ttcttgctat tttctttaat 181501 taggtaattc ttacatatta aaattctcat ttcatttcag gtagataaat tcctggctga 181561 aactccccta taatatggtt tttttaaagc tatcagtaag actatgtaga atgttctcaa 181621 agttgggcta tgaaacatca gagactgaat aagtgaaata agattgattc atttcaaaat 181681 ttcaatagag atttattctt tgagtaaaat aaacaatttg aagcccaaac tatatgccaa 181741 caaatttgac cttcattagg taaaacacaa cagcttgtgt tagcttacat ctctatctta 181801 acttttgaag cagtcattgt caagttttga taatgggaac cctgtgggta aatacatttt 181861 ctcaatagcc tatcatactc cgtaatagca agttttagaa taaaggatgt tcagaaattt 181921 ttaaccatgt actagaatga tcacgggcac ttgaagataa acttgtaacc tttttgaaaa 181981 agaaacttag ggacatttgc tattttgcat acgagcaaaa atgagggggg tattagatta 182041 ttcccggaac aaaagtctaa caataacctt aagatttata gagggttttt aaaatttttt 182101 ctagaagtta tcaaagaaga aaattccgca tttgcgaaat gaaacaaagt tctttccaaa 182161 tttctttggg tatccaaatt tttatcttgt tatgatttat cttgttttat cttgtttgag 182221 tgtgcacata tttagtgtgg ttttgagttt gcagttttgc tcatacctgt gatgtattct 182281 tcatgctctt catatcgttc atactcccgc tcctcgtatt cttcatattg gtcatattct 182341 tctgttggtt catactcctc aaattcttta taatcatatt cttcatattc ctcatattct 182401 tcttcccgtt gtactgaaac agcttcttct tctagggtat aagccctttc tttctcttcc 182461 attatagtta cttctgaaac aatattaaca acaggcagca ctttacttta aagcactttt 182521 aaggatgact gatagtaaca atgtgctttg attttattct tttgcgtttg tttcatgcac 182581 tgattatggg agaccctgac tgttcttttt tggccagtca aggtatcata gagtatattt 182641 agcaattgtg atttcaaatg ttatataccc tgcccttaat caacaataag ctaataaagt 182701 atRggagtca gattgggaac tagaaggcaa agagccagat agtttcatgc ctcacattac 182761 ttaatcaaag ttcaatatac ctttgatggg ttgaggttct ctttttggag cttcaattga 182821 tactttttct tctttaacca ctctttttct gaattcagtc actttaaagg agtaattatt 182881 aaaagtgaat tgcaagattc tgaaaatgtg tgtgtgaaga gaaattttgt tcaaacaatt 182941 caaggacaaa gacaaccata acagtgcctg attaaacttt catttaaaaa gcaagaaaat 183001 caatagactt ggaaataaac ttactctaat atacatatga agtacgtcaa agacattgaa 183061 taattttagg aaaatcaagt agtatttata aaagtttaaa aagacaacta aacagcataa 183121 ataatgacta tcaaactaag tagaagcatt agacagaaac ctatgttaca tgaatagttt 183181 cttcacctca cttctaagac attttcctta gctctattgg tacaatgtag aaaattcaga 183241 attgaaaaaa ttaaaaattt catcagagat tgccacataa agcaagcatc taggcagatg 183301 agtttagtat atggaaaaga cattagaaga caaatacaga ttaaggaaaa ttattcacat 183361 acgtttagaa gaagcctcac ttttttttaa gaaaaaagta ctttctgtta tcaaaaatgt 183421 ttagttttga agtgatatgc attaacaaac ttatattgct tactttatac tatgtctttt 183481 tttttctaat ggaacctatt ccactagatt agttttagtg tctggatgct tattttgatt 183541 tttttttttt ttttaagagt cagtatacct ttagctggtg gttcctcctc tcttttaggt 183601 ttgagtttca gaactttttc ttctgggaca gctctcttcg gttcctctgg cactttaaag 183661 agagatttca ctttaaagta ttgtttccct ctttcaaacc acaaaaaggc agccataaag 183721 tagtagccca aacataaaca cttgacattt tcattatttc aaacataaaa caacaactgt 183781 aacaacaaca acaacatcaa caacaacaac aacaacaaaa accagccccc ataaccagtg 183841 tatttggtaa agagagtagg gcaagtacaa tattgtgcat aatggaaggg cggatgtacc 183901 tcttgctttt ggaggcgcct cttttttagt tacagcaaca agaacttttt cttcctgggt 183961 aatttgcatg tgcctctcag tcacttaaaa gataatttta ggattaggga gttatatcaa 184021 agtggacgta aaaaatatac tagccagaaa gtactgtgat tttagaatag aagaatttac 184081 aaggcacaca cacaaaaaca tcacaagtaa tactctaccc aaagacacca ttgtcaccca 184141 ccaacatata aacagtatga cccaaagaac agcagcagag agaaagaaag acaagccata 184201 tgttcctagt ttttctgctg gggaggtttg tttacctttg gctgggagag gttcttccat 184261 cttaatgact tttggaggaa cctttttttc tggaactggt ttctttggct cttctggcac 184321 ttaaaagata ccaggcaata ccatcaaaca tacgatatgg aaaacactaa acacaggcac 184381 acttattttc tggttaatgt ttaaaaaatc tgaagaatgt atcaatttaa gattaaaaaa 184441 agacagtctt gagaataaaa taaaatccaa tataccttta ggtgggggca ccttttcctt 184501 tttaggaact ggagcaggaa ctttcttttc tggcacaatt ttcttaggtg cttcaggaac 184561 tttagaaaga ttaggtttaa gaatatgagt ttgccttaca tatgaagtga cacagttgtg 184621 gggcttagca ttcacttttt gaaaagattg cacatatata caaacgttaa tgactatact 184681 cagtaaatac gttcatacat gtgttctgac aaaacgtaga cagttatgca aattgttatg 184741 cataagacag ttatgcaaat gtgaaggtat tattatatac attaaaataa atagtgttgc 184801 atttctttga aagaaatcat acctttagcc ggtggaggct cctctatttt agcaggaatt 184861 tttggtttca gtacaatttt cttctcctgc ctctctgtca cttgaaaaga ttataaaata 184921 tgtttgtaga aatgtctaga tttcttttcg ataaaagtgt aagggatctt tttattagac 184981 atgatattta tcaatatttg caatatggat gacaaattag ctataaggaa acataaagaa 185041 ggagacaagc taacataggg ataaaactaa ttaaagagtc agcatacctt cagctggctc 185101 agcttccact ctcttagaaa taatgtgcag cttttcttcc acaacatatt cctcaggctc 185161 ttccatcact ttaaagacag cagttttaaa gaagataaat tacagtgttt ttcatgttta 185221 tttttagctt taatttatca cttcaaagag taatcaaaat agcaaaagtt taacccttgc 185281 caaacccctg cttgttccta tttaaaatac tcaaagagca ttttaatcaa agcacatgcg 185341 ttaatgaaga gtatgggaaa tgggaaatag gtttcattaa cttaattttc aaatgtgtca 185401 tacagataat aatgcttata tccacagcaa cgacaggaca agtacgggac aagaaacgat 185461 tataatagct gagttaataa gaacagaaga atttgaagat gagtttcaca aatagcagag 185521 cactgtagat ttgaaggcca cagggcagaa acatacttga ggatttaagt gaattgtaag 185581 ataatattcc atattgtttt ccccgccagt tttgtggggc aggtatggta gaagaggcag 185641 gaggcttcac atgatttttg agcctaagtg tatacagttt taattttttt tttttttttt 185701 ttttttttga gacggagtct cgctctgtcg cccaggctgg agtgcagtgg cgggatctcg 185761 gctcactgca agctccgcct cccgggttca cgccattctc ctgcctcagc ctcccaagta 185821 gctgggacta caggcgcccg ccactactfcc cggctaattt tttgtatttt tagtagagac 185881 ggggtttcac cgttttagcc gggatggtct cgatctcctg acctcgtgat ccacccgcct 185941 cggcctccca aagtgctggg attacaggcg •tgagccaccg cgcccggcca gttttaattt 186001 tttttaagac aatgtcttgc tttgttgccc aggctaaaat gcagtggtgt aatcacagct 186061 cactgtaacc tcgaactcct gggttcaaat gatcctcctg catcagcctc ctgagtagct 186121 gggactagag gtgcatgcca ccatgcctag ctgttttttt aatttttgta gaatggggct 186181 tgctatgttg cataggctgg tcttgaactc ctgtgctcaa gcaatcctcc cttctcagcc 186241 tcccaaagca ctgggattat aggcatgagc caccatgcct ggtcaaagtt aaataaggaa 186301 tagaagtgtc tggttaaaaa catatctgct ccttacgctg aaagtaaacc taacaagcca 186361 cccatctttg gggcataatg agaacataaa aataatcaca gtgtttctcc ttttaactag 186421 agtctggagt actacaggaa gtttatagtg tcaaaagttg aaatcagact atgtttggtt 186481 ttacaaacac ttttagaaaa gatggagtcg tcttcaaatc taccagcttc ctgacttatg 186541 tccaccatca tggcctcttc caggaagcac aacagagtta atggatttcc tcctgggagt 186601 catgtcccat cttggcctat tccaaggcat tccactaaga ctatggcacc ttggattttt 186661 acatgggctt ttgttccaag tgaaaaaagg ggcatggtgt gccatctttt attagacaga 186721 taaaaacaga tacttgggac atctaaatgt acttcaattc tactcatctt ctcactgggg 186781 agagagttag tttggttaag gagcttattg atttctcttt aaaagcagac cttaggaact 186841 ttcttaggaa agttcttttg gccacatggc tctagccaga atgctggatt ttagttggaa 186901 acctggtgct gccttaattc attgaaaaaa tatcccaatg gaatagtttg ttggttctgt 186961 ccttaagaaa ctatagataa ttagcattat aggtatgaaa agatctgagt aacaagtcag 187021 tctgatccca tatataacac aatggttttc tccatatgta aaatacagga aagccttatg 187081 tgttcagggc ttcagactca caaatatggc attgactcct ggaatgtaat gaggtaatat 187141 attttcttgc tcattttaaa acattttaag acattatagt gacttctgca attgacttaa 187201 tggattataa cttgtatttt ttaaatgcct agaataaaaa aattaaagga ttataatata 187261 aaatatattt ctgtctgtgg attgtggtac aagcaatgtt tgagaaacac caattaggca 187321 ttaggctact aaggtaaagc ttatgccagc ccaccatgct gaagccaagt cctgtttaat 187381 gaaaggatat atgtatatgg agatacctgc cctgattaat tatatatMaa tatgaagcct 187441 cttttattct atgtaggcaa gtaagaaggg atgcagaaga aatacactgg atttctctaa 187501 tctagtataa tcctcctgaa tcttgtgttt tgatcaaaat attcatttaa tgagactggt 187561 agatgttcac tgaatttgtg tcactttttt ctgtctcttt tcattggtct gtagacaatt 187621 tgtgaaagaa aacacctcat caaaacccca gatcatctct agcttatttg cattgtttac 187681 cttcatattc tgtaacctct gcttcttcct cctcctctct ttcttcttct ctataaactg 187741 aaatggacac accttcctcc tcttctgagt aactccattc ctcctctgca gatactttaa 187801 aagataaggt ttcatttaaa ttcagcctgc tgagatacag atgtatatac agccatgtgg 187861 tcactacaga tggataactg ttcttctatc tgcttaggac atagcttcat ggtacttcct 187921 cactataaga aggaggaagc taatttaaga tgagggcaag tacagcccaa gactgagaat 187981 gatacttgag ttatgtccaa gtgtggccac tttcaaaatt ataacatttt cgggactcat 188041 ccacttcctt cgaatgattc aaggccaaca tagacacaaa agactcagtc ccagaaggct 188101 acaaaaggta gacacattct aataccaaca taaggagaaa taactgatga ggacatgttc 188161 taaagtaaac aatcacatgt ggaagaagaa gagactttga ggaaacacac acaaactcat 188221 ttatcccctg acttccgatt atataccttc gtgccgcgtg acttccactc tttgaggaac 188281 tgcgaaggat agtttttctt cagcaacaaa tctcttttct tctacaacag ttttcttaga 188341 gacttcagct ttaagaaagt gttaaagttg aagtttaaaa tcaagaattt taacaattct 188401 cactttctag aatttgggga agagttgcac agtacaccac tttgactagc aaaatgggaa 188461 aacaagttac atatcacaag gacataaaca caaaagtttc actgcaagtg agacaatgga 188521 tcagtataag cacagaccag atggaaaaag caagaattta acacactcga agattttttt 188581 tttttttttt tttttttgtc agaggattga ggcaaatcct acctcgggga ggaggagctt 188641 tcttagcgac aggaactggc actgcaactt tctcctctgg gacgggtttc ttaggcagag 188701 ctggcacttt agagacatta tgcactttta gaaatttaat gtgatctcat tgaagcccaR 188761 tgcaaaacaa agaaatacac ccacagtgca ctcatatcac aaaactaaca aaatcactgg 188821 aacataaaga cagttcttgg gaagatggag aaacaatacc ttttggtggt ggtggaactt 188881 cttcctcctt ccgaggaaca ggtttccttt cttcaggaac tttcttcttt ggtatttctg 188941 gcacttaaag gatagtattg aattttaaaa tttgtcaaaa aggccaaata aatgagcaac 189001 atagaagtgg caattaaaga agacatgcaa ttaaagaggc ttgaaggaaa gacaaggtta 189061 tttcatggag atgggattaa gtacctgctg gtggtggaga ttcctctctt tgagttacaa 189121 tggttatttt ttcttctgtc acaactcYct tctgtacttc aggaacttga agagacattt 189181 ttagaattga ctttatattt tctaaagtag ctatgcacag ttattttttt taagaaagca 189241 gacgggtgga cagacacttt tgttcagttc tcaccacaaa acattcattt agaattaaac 189301 taactcaatg aacagataat taaacacaac atattttgta tcaaagataa aagatagggc 189361 tttacgtcga aagccactgt acctttagct gggggagctt cctttttctt tgcaacagga 189421 acgggaatct tttcttcagg gaoaggtttc tttggcacct ctgggactta aagtttttga 189481 aacacaatgt tagttcagac atatatcact tttatgaaag aacataggca catgcacaaa 189541 tctttatgta gtacattaat gttgttatgg attatctatg catcagcaaa attatccaga 189601 aaattaaact aactatacta ttccaaggat cacaatgtga taaatatcac aaataatgtt 189661 ttagaggcat ttaggcaaaa atgagttatg taacaaagtt gtgcacagaa ttcatccttt 189721 atatattgta tgaccttgct aaaaacaaat tagtgatgac acaaacaatc aagaatgtgg 189781 atatggtata tagaaaactt ctttacgctt tgtattgaga aatggagtac ttaataacaa 189841 tggctttagt ttatctaaga ctattttaga ctaagactaa gaagtcttag aatagttgaa 189901 tatgtgtaac ttacttctct tccccacatt tctcctctca tttttggggg gtgtgcctgt 189961 agtgaattca aaactcagga aacactctag agtaggtctc tcaatctgat acccatgtgt 190021 taatgacttt acagagcttt gtgtgcagaa ttttgtgggt atgtgtattt gtgctttttt 190081 tttctaaggg gctctagagt tttgaacagg gttttcaacc taaaaaatgt ttacagtgta 190141 tttgatttat ctattctaag aatggataat gttgactttt taattggaag atgaatttta 190201 acagcatgca ttattggcaa taattgttct cattttaaat acaatattgg cagcttttgt 190261 gaatccctgc acctctgcac gtgcatttgt aatattttgg tgcaattata tagctgttaa 190321 tttatatatg tatatgttat gctatatata catgcagtat ttatatatat atacgtgcta 190381 ttatactatg tgtatttgtt tttatgagta aatgagagat gcctctgtgt ctcctttaga 190441 agacatatat gactttgctg aaaacgaatt agtgattaca caaacagtca agaatgttca 190501 gatagtatat agaaaaggtt ctttatgctt tgtatgggga aatgtagtac ataataacaa 190561 tggatttaat ttatctaaga cgatctgtct caagtagctt tagtattagt attggtttta 190621 ctaacatgac ctttgtattt aatggttaga taatcaaaag aattttgaaa gagccaagag 190681 tttgagaaag agagaagcat atttgattca ggaagaaaat agaaacattg caaagtcagg 190741 ccaagggaac actattcaaa atgtactatt ttagttaggt ctggtttctc ctaaattgga 190801 taatgttgaa ttcttgctga gtctctctat aactgaatgg atgcgtgcaa ttgtagcttc 190861 agtgcttcaa itaagatagtg ccttttatcg ccaaaaaaat cccaaacaaa ccaaagcaag 190921 ggatttgtga tataccacag cagcacattt tcattcaaac atacctctgt gaaaggtact 190981 ttgactagct aattagataa cggaaacatt tatggcaaat actgaagctc agttaaggtc 191041 tcaagaaaat ggtgaaagta aacttcaaaa aatctgtatg ttccccacta ttgaagactc 191101 gccactattg acttttaaga agcgtggtac tatgtacgtc tttatgtaat taacattcag 191161 ttctgccaga cactctccag ttgtcattgc ctgtctccac ctcttcataa tgcatgaaac 191221 tgcttttatt aagaaaaagt tacatttatg aaggatgaaa ggttttttcc tagaaacatg 191281 tcagtagaaa acaagtttca caaacatttc ctctttctct aaaagaggga agatattaaa 191341 aatgtaagat agcagtttga catagttact tcctttaaca atactttttg gtgatgtctt 191401 aggaatattc agtataataa ataaagtatg tcaagagcat ggcacggaag ctgacaaaca 191461 gtaaaaacac aagaagaatg taaaagagac aaaagacaaa ggcagcatag tacatatgaa 191521 gatcgtagaa agcaaaaggc tggcacttgg agcJaaagagt ctccccatca ttggctctgg 191581 cgtacctttt gggggagcag caggttcctt cttaggcaca ggaactggct ttttctcctc 191641 tggcacgggt ttcttgggaa cctcaggaac tttaaagata caattgttga aataatgtgg 191701 aatattctag tcaccttctg agacatctaa gattacatta aagcataaat cttcactaac 191761 agtgtgagaa ttaggaagta atttaaatat gcctacttgt ggtagaaatg aaagctaaat 191821 acctcaacac catatagaca ttacatacat tacacaagca gacaaagacc cagttagcgt 191881 gatctggagt ttagaaatgt taccaatact tgtataacac atcaccacaa ataaatgaaa 191941 tcaatataca cagaattaaa tatattatag atggtttaca gatgctattt tgttaataca 192001 cagatcttat agaaaaagat acaagatgga tgctaagaat tatttttttc acaaatatta 192061 ttctaccttt tggtgctggg ggctccactt ttttagggat aggaacaggg gccacttctt 192121 ctggagctgg tttctcaggt agctcaggga ctttaaaaga aaagtgccat ttttgagaaa 192181 gtgtgcattt gacaaggcat atgttgttgt aagacttaga attaaataga gattcccttt 192241 ttataccaac tgaatgcaag aaaattatgc tttagaaatg agagtaaatg aagatggctg 192301 aatggctaat tagaaaatgt gactgctgtc acctttaact gcagaagcct ctactttgtc 192361 aggaacaggt aggagcaatc ctccctcgga ggtgcctgtg tcagttcctc agaaaccagc 192421 tttttatgaa catcgataac tatgaagtta gttttttttt ttgtaacctt tacttttata 192481 aagcagtttc ataaatacaa ttgcattaga tttttttgtg tcttaaatgt ttaacaattt 192541 gggtgggatg gagggggtgg agccttgatc tatagtgctt gcagatttcc gatttccaag 192601 gtggtataaa cactccctcc caccctggct gtcaaggtga tgtcattgtc ctggagttgg 192661 aaagagatgt gcagtagtac attatatggc atttccacca cacagacaca aagatggaaa 192721 caatctcaga aacacagata agaacaaaat gcagtaaaat aggagttttg agtatttatt 192781 aatatttaaa aatatacttt attggactgt aaatttacat aatctaattt ttaataatgg 192841 tggtgttaaa ctactgactg gcaaaattcc tgaaaatgta accataggct ctggtgagct 192901 agcatgaact ggctccagga cactgtattt ccaagtaaca ggttacgaga ttgcaaactc 192961 tctgaagcag ggaatggagc ctcccataat tttcactttt ttccccctgt gccttcctca 193021 tggttcttcc cgtcccccat cacatccttt gtacatagtg gctataggta tgcacactgt 193081 gactaaatct attatttaat actaacataa atgttttact atggtatttg tactaatttt 193141 tattaaaaga gtttaaactt agaatgaatt actaatacct ttaggtggtg gttcaaccct 193201 tttggaaaYg gcaacgtgaa ttttctcttc agtaacaatt tccttttgta cctcggggac 193261 ttaaaaaaat gtacatttta attactgata tgccatgttg tgggtggtaa ttgtaattta 193321 caaagaacat taaaataaaa atgaaaacag agacaaacat gaaatgaaaa agatgacaga 193381 atttacaagg agacagaaat gtctacacga tcacaaatta gacaacaaga gggataaaaa 193441 tctgcctaaa acccttccat ttgagccccc acattccagt ttgccttata cctgtgactg 193501 acacctcctc ctctgtgtga gaagcaaaga acatcttttc ttctgaaata accatcttct 193561 ttgtatgcac agctggtact ttaaagagag tatttcacat tagtattcct tttttttagt 193621 acaagacatc agatcaaaca catggatttt atactcaagt atttagacac agaatgagtg 193681 ggttaatatg gtagcttgag aacaaattca gtattgacaa gaaaacaaat catgttttga 193741 tctataggga caaacaatgc atgcagcctt catcaaacat tcttaaatga gaatcaaata 193801 gacacaactt cttcacacac aatgttgtgc ttttccaaat ctgttagggt ctatttctat 193861 aaataagagg acagggaata ttatatgcaa tggaatgcat tgcatagtga agttagcaaa 193921 ctactaatta aacacaaacc ttctttataa taaaaaaatg taatttttaa gcagaactag 193981 aaacaaagta gataatgtta ctatttaaat taaacatatg tgcaactcaa cataaaattt 194041 gtattatgta aaaatgtgct gtttttggtc gtttcagatt tgtgagttac ttagcaatat 194101 atgtacacat gtccatacac ataaataaaa aaattttgaa cttgtagctg aaacacaaag 194161 atgtatacct ttcacttcaa taacttcttc ctgtactgga gtccgggaag gtttttgtgg 194221 aacaatcttc tttgaaactt caggtacttt aaaaatatgt acaaatatat ttgtattttg 194281 aagaatattg ctttgcacaa aatttaaaag tatttgatta tataaataac taatgtgagt 194341 atagatcagt aaacatatta cttgtgtaca tgggtgctag gaatgtttta aataatacct 194401 ttggtgactt gaactttttc ttcctccatt cttogagaag tcttttcaat tttttcaatt 194461 actggcttct ttataactgc aagcatttta gagaaattgc agtactttta gaattcctat 194521 taaaattctc tctctctcta tctctctctc tctgtgtata tgtgtttata cacacctata 194581 agtgtatatg cagatacatg tatcattttt taaagagaga gatgagttcg gttttttttt 194641 ctttgacctt tacgcacaac tttgaactct gggtacaagt ttctgtactt aacatatttt 194701 ggttatattt aagtttacca ttacaatttt tatcatcttt tgatttttta atgaaatcaa 194761 ttagatagaa attttaagaa aaaaaaatca ttcacaataa agcttagaga agaagttgtg 194821 cctattggat tgaactgctg atttatacat tgctgccaaa caagccatga taatgatgtt 194881 gatgctctag tctatttaaa tatttctaat tacttacctt taggaggtgg tggtgctttc 194941 ttttcaggta ctttggctgg aacttttctc tcatgtgatt ctgaaataaa aacacaggaa 195001 taagaaggga tgcttaaata ggtaagttct cttaggttgt taattgcaaa tgagataagg 195061 taaggataat atataatcgt gtgaagtatt atatttggga tcgttattac caacacaatt 195121 cttagtgcta ggatttgaag caggatcaag cagagatgca acaaggcagt aaaagtaaaa 195181 cttgaacttc tgcattagaa attagcactt ggaatttagt gttagtaata- agactaaatg 195241 atttagcttg taatgtcaaa gcatgaaagt aactctgagg aaaataactt ttaaaagatt 195301 tctaacctta aaaaaatcta agatcatttt aactatttca cataaactgc aaatcaggtt 195361 catagcattg aaaatttaat gaactgagca aaaatgaaga aaagagggaa acaagtcatt 195421 cagtttatac ataccttcat agacctcctt ttgaacttga attacttccc tttcttggta 195481 agcctcttcc cactcttcct ccccttcgtc atagccttct tccctttcat agtattcttg 195541 cccttcttca aaatcttctt cccattcctc gtgaacttct tttttagctt ctaccttaat 195601 ctcttcatag tcttcatctg gttcttcata ataaggttgt tcaaatggot ctgtgtatgg 195661 ttctacctcc agttcgtcat aaggttcttc gaaagattca atgaagactc tcttctcctt 195721 gtggactgct tgctttttct catacacagc ttctttttct ttctgaacct ctttctttct 195781 ggttatagtc attattttta cttcttcagc tttagaggat acatcaatga tttcaggagc 195841 taaaatagat aaagatacta ttagcatatt aattctatcc atccaactgc ttcactaaac 195901 aaaaatctac ctagttaaac aacatcagta tttcagatct attttattga taatttgttt 195961 tttttttttg tattggttcc atttaaattt ttgactatgg aacatagtag agtatcaaga 196021 tgcattctac tcttgatttg aggatttttc cagtatgaaa. aaatggattc aataaatatt 196081 atcaaatgga cacaccttct attattccat gagagctcca gtttgcagaa cccctagttt 196141 cttccagtgg aatagtttta atattcgata agttcatgga atttatattt catatttata 196201 cagttaacct ttttaaaaag ttctgattat tatttcaaaa acatttctaa ttttaaacct 196261 aggtttttga ctattgcata tgatccctta gtcagtttca taaaaacaac atttaaaatt 196321 atgtaaccaa gttaattagg tacttttaga taacagattt ttaaagttct taagggctct 196381 cattatttat atgactctta acatttgtgt taactacaat aaaaaaataa ggaatggccc 196441 ttcctttcta gccctcactt cttctttctc tgagaccatt gcacagaggc agcataaaca 196501 tctgcctggg tttgttttcc tgggaaaggg gcaatatgaa atagccatca tttctaatgc 196561 taaatttaac acagcagagg agactccaca actttcaata agttggaagc ctaatattta 196621 aaataagaat agttagcaga agtgcaaatc tcaggaataa acagaataaa aataatttat 196681 ctttatttac cttttgctgg aattaaggta gtaggaggtg gaggcttctt gacaatctct 196741 gggagtttaa aaacataaaa atgtgtgttt atttttagat tacattatta ttaatgttta 196801 ctaatcctcc acatcattgt tagttgtttg taggagctat gactactact catacaccag 196861 cccttccagg aatcataata atgcttctat aatttccaaa tctagctcat tagaaatgct 196921 atgcaaggcc atattaacaa aaatcaaaca tgcaaaaggc caaagtttga ttctagttta 196981 gattgattta agatcattta aattggttct agtttagatt gatttctaat aagttaacat 197041 taagcatgaa gagaatgttt aatatgtcca atgctaaaaa taacacatta gactttttca 197101 attattaact atgaagttca tgaaatatcc atgctttttg agtgtcctct tattccatga 197161 tgtttcacaa atgaccactc agaaaagtct gaatgatgaa tgatgccttg tgtttccata 197221 ttttccttct ttatggaggt tgctgtactc taatcttagt caaatgagtt aaaactattt 197281 gtttaaattt agttatccaa ctgttttcct aaaaatcttc gaggatatat tgaaagttaa 197341 agtgatttga attgatcctc cttcataaag tattttttct tcaaatattg atatttagat 197401 cacctaaatc aggaaagata ctgaggtggg ttgcagcaac ctcttaaaat tagcagcaac 197461 ccttgatctt acattgatgt gactttatca agaccagctg ttgggtactt ccatgttcat 197521 tgcagcatta ttcacaaaga ccaagttatg gaaacaccct aagtgtccat caatggatga 197581 atacataagg aaaaggtggt atataaatac acaatggaat actattcagc cttataaaaa 197641 aagggaattt tgtcatttgt gacaacatgg atagaactgg agattatgct aagtgaaata 197701 agccaggcac agaaagacaa agactgtaag ttctcactta gatgtggaac taaaaaagtt 197761 gaattcataa agagtagaat gatggttaca gaggctgggg atttgaggga atggggaaat 197821 gttggtcaaa gggtacaaNN NNttagacag aagaaacatg gcttttcttg agatctgttg 197881 cacagagtgg tgaatataRt taataatagt gtactgtaca tttcaaaatc gctaagagta 197941 aatttcaaat attctcacca caaaaatgtt aagtatttga ggtgatggat atgttaattg 198001 gcttgattta attattccat attgtattca cagatcataa catcactttg taccccataa 198061 atatatacac aatagtaaat agtcaattta caataaaata
[0377] FollowingisagenomicnucleotidesequenceofaLOCI12609region(SEQIDNO: 3).
>6:84746151-84845550
1 ttttggaaaa tttgcaacct gacaatgtgg tagaaaagaa aaactcattt tctgggagaa
61 attcaagcca gctgcagaaa tttgcataag taacaaggag ctaaatgttg attgccaaga
121 caaaggggaa aatgtctcca gggcatgtcg gagacctttg tagcagctcc tcccacccat
181 ggcctggagg cctaggaaga aaaaatggtt ttgtgggctg ggttcaggcc ccccctgctg
241 cgtgcWgtct agagacttgg tgccctgcat cctagtcatt gcagccatgg- ctaaaagggg
301 ccaaggYata caaaggggcc aaggtatagc tcaggctRta gtttcaaagg gtttaagccc
361 caagccttgg cagcttccat ttggtgttga gcctatgggt aaacagaggt caagaattga
421 ggtttgggaa cctccaccta gattacagag gatatataga aatgcctgga tgtccagcca
481 gaagtgtgct gcagggacag agccctcatg gagaacctct gttagggcaa cgcagaaagg
541 gaatgtggag tcagagccct cacacaSagt cttcagtggg gcactgccta gtggagttct
601 gagaagaggg ccactgtcct ccagtcgcca gaatggtgga tccactgata gcttgccccR
661 tgcacctgga aaagccacag acactcaatg ccagctgtga aggcgccagg agggggctgt
721 aScctacaaa gccacagggg tgaagctgcc caaggctatg ggagcccacc tcttgcataa
781 gcatgacctg aatgtgagat atagagccaa aggagattat ttcagagctt taagatttaa
841 ttactgcttt attggatttc agacttgcat ggggcctgta gcccctttgt tttggccaat
901 ttctcccatt tggaacagtt gtatttaccc aatgcctgta cccccattgt atctaggaag
961 taactaactt gttttttatt ttacagactg ataggcagga gttgccttgt ctcaggtgag
1021 actttggact tggacttttg agttaatgct ggaatgagtt aagactttgg ggaactgttg
1081 agaaggcatg attgtgtttt gaaatgtgag gatatgagat ttgggagggg ccaagagtgt
1141 aatgatatgg ttaggttttg tgtccccacc cgtctcatct tgaactgtag tgctcataat 1201 ccccatgtgt tgtgggaggg acctggtggg aggtaattga atcatggggg cagttatccc 1261 catgctgttc tcctgatagt gagtgagttc tcatgagagc tgatggtttt ataaggggct 1321 ttcccccttt tgctcacact tcttgctgcc gccatgtgaa gaaggatgtg tttgcttccc 1381 tttccaccat gactgtaagt ttcctgaggc cttcttagcc atattgaact gtgaatcaat 1441 taaacctctt ttctttataa attacccagt ctcaggtatg tctttattag cagtatgaga 1501 atggactaaa aacttccact gaattatccc aatgggagag gagggttaat ccttatttct 1561 aggattctga agcagaggcc aaaggtggca ggagcaaaca tttatttggt tcttacagtt 1621 tactctgtaa catatctgca cttatttaat ataaaacata atcctccagg tcttgtatta 1681 ttgatgctcc aggaagatga accaggtatg ccctgagact ctggggacta tttgggaccc 1741 ttagaccttg gcaagctgag ggacactata ttcttgccct cactttataa agcccctctt 1801 ttctggccag gcccctccct gaaagatgag gaggctatgg ggtgccttga gcctgcactg 1861 cccttgcacc ccaaactctg gtttgcttgt aattgattgt cctgaggttg cagtgggtgc 1921 agtaggtgga gaaaccacaa gagccacaca tcctagttta agagcccagg cctcagaaga 1981 taagatggtc attagaacgt tcagcttttc tactgctgtg tctcaagctc ttaaataaac 2041 cactgcttta acttacattt ccatttctgt ggaacagagc tggaaatacc tggtacctct 2101 ccttttcact tgtttgctaa aagaatggtt cagctcagga ccataataaa tagtgtgtga 2161 tatggtgtgt gctgtttgga gacagatacc acaagccaaa aatacaaagt agtcttgttt 2221 ggcaagtcat cctcacagtg atttaagaca cggccagtct ctcttcttag tcttattctg 2281 ccaactcttt caataactcc actaattaga acttaaagag tggttgattc cctggtattc 2341 ttaaaatggt ctaatttgaa agtttaaaat ctttaaagtt ttaattcacc tagtcaggcc 2401 gttttagaat agcagctata taaaacagta tgaggactca gtgagaggcc aagtctagtc 2461 tttggggagt ctgatgtata ctttcttgct actaaaatga gagattcaga taatccacat 2521 aatgggattt aagagaagag agagatcctc aatgtagtga aatagttgga gagatctact 2581 tggaggaggt agtatctgat ccctaccctc aagggtagac aggaatctgg aaaggaggga 2641 gaggaagtcc agcttgcagg tggaactgaa tgggcaaaag ctcagaggac cattttgagg 2701 gttcccccgc tgcaggttcc tgtaccatca tgagccctcc cttttttaat gaccctgttg 2761 tttctactgt ctatgtcttc aggcttttgt gttgtccctc aagctgtgtg tgtatggggg 2821 ggtgaggggt gtggggtgag tgggtgggtg ggtgtatttg aaagaatgaa gttcattgtg 2881 tggSagagga tagtttgaaa tacaagcctg aattctttaa gagcacagtc cacagtcctt 2941 gaatttttga cctctactaa atctttcatg gttaagtaac agtgtttaaa atatccttgt 3001 tgatctgaat ggactcataa taattttact gtgttcaaaa ctagtgctct ttctctgtga 3061 agggcatatt tatgagaact tagtagatgt gtttgctgaa gacggcatgt agtgggatag 3121 attaatgaca ttttgtaaat gttttctttt caaaaatgta ttttttttta gttatagtta 3181 gagtgattgg tacaaagtaa gtcattttta atggacttcc taggacagtt taattagttt 3241 accaaagaga acattgtttt ctccaaatta gtttcagcac ctatatgaat ttcctcgggc 3301 tgccataaca aaataccaca gactgggtag cttaacctgc aggagtttat tttctgtttt 3361 ctttttttga gacagagttt cgctcttgtt gcccaggctg gagtgcaatg gcaYgatctc 3421 agctcaccgc cacctccgcc tcccaggttc aagtgattct cctgcctaag cctcccaagt 3481 agctgggatt aaaggcatgc gccaccacac ctggctaatt tttgtatttt tagtagagac 3541 gaggtttctc catgttggtc aggctggtct tgaactcccg acctcaggtg atccacctgc 3601 tttggcctcc caaaggaatt tattttctca cagttctggg gactggaagt ccaagatcaa 3661 ggtgctggtc acatgtaggt ttcctctgat ggccataagg gaagctctgc tccaggcctc 3721 cctccttggc ttgtagccgc tgccctcttg ttgcctcttc acacagttgt ccctctgtgc 3781 atgggctccc ctgatgcctt tctctctgtg tcctaatctc ctcttcttat gaagatattc 3841 ttYagaatga atgagggcct cattttaact taattacccc tttaaaggcc ctatgtccaa 3901 atacagtcac attctgagat actgggtgct agggcttcaa catatggatg ttgggtgtgg 3961 ggacacaatt cagtacatac ggcaccttat tcacattgta gtacagtcgg ggctccacac 4021 ccgtgggttc cacatccatg gatttagcca attgtggatg gaaaatattt aaacaagaaa 4081 aggatagttg actagatttg aacaaaaaaa ggattctgaa catgcacaga ctttttttcc 4141 ttgtaattta tttccttaat aatacaggat agcaactatt tacatagcat ttatattgta 4201 ttaggtatta taagtaatct agagattatt taaagtatac cagaggctgt gcataggttc 4261 tatgcaaata ctacaccatt ttatactagg gacttgagaa tctctggatt ttggtatctg 4321 agaggtcctg gaaccaagcc cccacagatg ctaaggaatg actttatata tatacacaat 4381 gaatgatgat agtgacgctg gacatgctgg acatcgggca gccaagatga acttgaataa 4441 ggtgttggtg gagctgtcag gtacagctga tgaggctgcg agggcagaga tccaaatctg 4501 cactgaaacc cttatgaagg ccctggagta ggcaagcctg cccccaagac ctctgtgctc 4561 tggagccacc tccacgaatg acctgccccc agccgtcacc cttcattgaa aatgaagaag 4621 cctgaaaaaa aaaaatatac acaSaggctg ggcacagtgg cttacacctg tgattctagc 4681 actttgggag gccagggtgg gtgaatcgct tgaggccagg agtttgagac cagcctggcc 4741 aacatggtga aaccccgtct ctcctaaaaa tacaaaaatt agctgggcgt ggtagtgcac 4801 gtctgtaatc ccagctactc aggaggctga agcatgagaa tggcttgaac ccaggaggca 4861 gaggttgcat tgagctgaga tcacgccact gcactccagc ctgtgtgagg agcaagactg 4921 tctcataaaa aaaaaagtat atacagacac acacacacaa tgtgtatgtt cacatgtgtg 4981 ctttcacatg catgtgaaca cacatacaca cccataatac aaatagatag agaccactga 5041 gtttgtaata caagtgaaaa gcaacttggt atttcgttaa gttacagaat catagatctg 5101 gaaggacttt cactacatcc cttctggttc ttttcctttg tttgtaaagg gggtgagtat 5161 ttcagccata ataggtagct ctcatgtgaa tgcagtagac agaggcagtg tgatgtgaac
5221 cgccatggaa gtgtctgctt gctgtgaaaa tattgatgtc agactgctag ccctcctgtc
5281 ttgcagagga ctgcctttat tgtgaaatta tatcttctct acttagaggg tgtttaagat
5341 aactgtctac aaaatgataa ttgttaataa tagttgtttt tttaaaattt tctttacttt
5401 gcaaaataaa aatgctggaa actctgtatt ccaggtccat gaaaactggc tggtctctag
5461 aaaacattcc ccagagactg gatggaacca gtttaatgaa attagggtgc ttcctttttg
5521 tcgttgggaa gctgctaatt ttcaatgtaa tccatgagaa ggacctgaaa gctttggaat
5581 gataatttgt agtgcacttc tcctgaaatg aattgggcta atgacaggta caacagaagg
5641 taaagcttat aaaaaggaga atttggctgg gtgcggtggc tcatgtctgt aatcccagca
5701 ctttgggagg ccaaggcagg cagatcacca ggtcaggaga tggagatcat cctggctaac
5761 atggtgaaac cccatctctg ctaaaagtag aaaaagttag ttgggcgtgg tggcacgtgc
5821 ctgttgtctc agctactcag gaggctgagg cagaagaatc acttgaaccc aggaggtgga
5881 ggttgcagtg agctgagatc acgccactgc actccagcct gggcaacaga gcgaaactcc
5941 atctcaaaaa aaaaaaaaaa aaaaaaaaaa aggagaattt gggaagtggc tagtgtgttt
6001 ccaggactgg tcagggtgag aggaccatga gctggcaaag cacacatatt ctcccacctg
6061 tctatgactc tgtcaggggt tgaaagggat gattcctcac agcttagagg gagtgttcct
6121 tttacttgaa agggaatccc cacagggctg ctgaaaagag caaagctgga tccttgaagg
6181 tggaccaggg aggaaggatc cacacaggga ggggtgctca gatgtctcag caccctgtca
6241 tcctctgggg ttctcttttg ctttagagag ttctcattac cctcccctca agtgctgtga
6301 tttggaagca ctgcttatta ttattatttt ttaaattatt tgtattattt aattaattaa
6361 ttaattactt tttgagacag gattttgctc tgtcacccaa gctggagtgc agtggcatga
6421 tctcagctca ctgcagtctc tgcctcccag gttcaagcaa ttcttgtgcc tcagcctttc
6481 aagtagctag gattacaggc atgcaccacc atgcccagct aatttttgta tttttagtag
6541 agacggggtt ttgccaggtt gaccaggctg gtctcgaact cctggactca aagcaatcca
6601- cctgccttgg cttcccaaag tactgggatt acaggcatga tccaccaagc ctagcctaaa
6661 aaaaaaaaaa ctataaacaa tattagaact tttttgtatt ttggttaaaa acgcataata
6721 taaaatgtac catttttgaa tgtacagttc agtggcatta aatatattta cattgttgta
6781 caaccattac aactatccat ttccagaact ttttcattat ctcaaactga aactctttta
6841 ctggtaaata acaatttccc atttccctac ccttagctcc tgataaccac cattctatta
6901 ggttggtgca aaagtaatca tggtttttgc cgttaaaagt atttgcaaaa accacaatta
6961 tgtttgcacc aacccaatac ttgctgtctc taggaatctg gctattctag gtaccctcat
7021 atatgtagaa tcatgcaata tttgtccctt tgtaactggc ttatttcatt tagcataatg
7081 ttttcaacgt tcatcaatgt tgtggcatgt ttcagaactt cattgctttt taaggcttaa
7141 tattgcattg tatatacaac attttattta tcattcatct gtgattagac acttgggttg
7201 ttttgacctt ttggctattg taaataatac tgttatgaac ataggtgtgt atgaatatct
7261 gttcaagtct ctgcttgtgg ttcttttgta tatatatcca gagttagaat tgctatatca
7321 tatggttatt ccatgtttaa atttttgaga aactgctata ctgtcttcca cagcggctgt
7381 acctttttac cttcctacca gcagtgcaca agggttccag tttctctata ccctcaccaa
7441 cacttatttt ctgatttttg ataattgcca tcttaatagg gtgtgtagtg gtatctcatt
7501 atgaagcatt tctttttgat agttgattct ttgacttgac actagtaatt taaagcatgc
7561 ttttcctaac agtttagaaa tagagcaaaa ataaaatagt catgggaacc cagaaaacct
7621 aactataata aaagtggctg gcttggtatt ctactgatgc cctcaacttg cctagaattt
7681 tttgaagtac agaccaagaa aagcaaggtc tagaaaaggc gagagttaag cctRtgcctt
7741 tcctgtgatg gggaacccaa ctcaaccatg atagcatgtt gtggtggcaa ccattgacac
7801 tggaataaag gatggcagWc taacacgttg acacacatgt agtgggatgg agcattaggg
7861 tcctgttttg tcgtattatt ataactccct tgtcctgatt ctacattcac cagctaatct
7921 tctgatctta aattttgttt cccagaccac gtaaaacatg tgctgtggtc agcttggcca
7981 ttgttttcct aattcaacaa atggatatgc tcattataaa tcattctgct atgcttgatt
8041 agcatgactg agtccataag ggctttctca tctctggaaa actgaaaatt cagctccctg
8101 aatctgtaga gccaggggcg gaggtgcagt cgcatctcag ggccatgcct cttctcttct
8161 ctggaaattt tctccagatg gagactttac agcttctttc ctggtgtgta acatgaaatt
8221 cttgatgtcc agctgaagtc tattagaatg ggactagtct gtggagtact acagagccag
8281 gaaacactca ctttaaaaat tacttctggc tgggtgcggt ggctcatgtc tgtaatccca
8341 gcactttggg aggcagaggc agtcggatcg cctgaggtca ggagtttgag accaaccttg
8401 ccagtgtggt gaaacccctt ctctactaaa agcacaaaac ttagccaggc atggtggtgc
8461 acacctgtaa tctcagctac tagggaagct gaggcaggag aatcgcttga acccaggagg
8521 cggaggttgc agtgagccaa gattacacca ctgcacttca gcctgggtga cagagggaga
8581 ctccatctca gaaaaaaaac acacaaaaaa caaacaaaac aacaacaaca acaacaaaaa
8641 acttactagc ctgtttacta ctatataggt gcccagggca taacaggaag atgtagattt
8701 atgaatccaa actgcttgtc ccctttgaga caccagctgg tgaaataagg gtgcccactt
8761 cttaattaag caattcttta tttcaggtgg tgactgaata tctatcagtg taaaatatta
8821 agtcccattt ctgtttttgg aagttgatct tctattggca ttgattttta tgttaagatg
8881 gaagaagtct tcaggcttgg gaacactttt KgttMgctgt cttgctgctg gaaacaagtt
8941 gcttatgtgc acaggagaag gggggatagg caggaaatag atacatcctg aattctcttg
9001 ttagctgtca gtcaccattt tttgaggact gctgggtaat tgagtacttt ctaggataca
9061 ctagagaatg gcataaaact tggcagtgta gaaaaatgtg ggaagtatgt ccatttgaaa 9121 acgaagagcc cagcgtgcgt gcattcatgt gtgtgtgtgt gtacgtgcgt gtaatgtctc
9181 tagactgaat tgagtggcca gagcatcaga cttcaaaagt aagagtcata ctcgagtcaa
9241 aatagtcgtg aggaggagaa gactacaaag tcctggaagc atgacctttt ttctttaatt
9301 ggaggagaga aaaatccacg ccccaggaaa gacatcactg acttaaaatg gattcacaga
9361 aaaagaatca gcagacctgg gaattaagtg ggaagtgcca gtagaaggtg atggttcacg
9421 cagactctat aaaaagcaga agtaaacttg agaataacac catgttactg tttttttaaa
9481 gtactgccct gggaggacac tgtggcagct ttgagagggt caagcatgat cccttgctgg
9541 gaggcattgc cttatagaga tgctttaaga agatccagct ggctcagagt gtgattcata
9601 accttgaagt ctctcatttt ctgtgtggcc ctaccctagg cacgagtaag ctgggtgcac
9661 cattgttctg tgcaaggagg aaattaagct tctaaagttg cgattctgaa gttcccacct
9721 tgggctcatc agccaccaaa gtgcagcgtg tctgagcttt atctttggtt tctcaagtct
9781 ccttgctgga ggaaagacag cgttatgagt cacaagggtg acttctctat gttgtgcggg
9841 ggacagtcat tcctggaagt aagaggaaga aggataagaa caggtaactg aaaagacctt
9901 tctggcctgg gccagatcag aggttggtgg tcctgggtgg catgcctact tttggagttt
9961 gaggattgaa gaggaaggtg agagagtcat tgcaattggg aactttgatc atctgactgg
10021 ccattaggtc ttcccctgta caaactggtc agcataggtc tttggatttg tggcactggc
10081 tggcagtttc ttggaatgcc ttagtagctg tgctgtcagt tgtgtgcatg tttgtgtggg
10141 ggcatgctca tttttcaggg tttctggcac cttttggatt tcttcttaat gtgctagtcc
10201 tgtgcctggg ttggtggtca cttgtaaaac tcatctgctg atgtttaccc cttaacatgg
10261 aaaaaaagtg tctaactcca aagaagcagc ccccttaaca tgcataccat cgtaatccac
10321 cagggatatt gacacaaaga catttattca ctctttgaga agttctatgt ctgagtacat
10381 ctcctcaaat tcagggaggt ggagagaatt gcttgWtcca tttttaagaa ggtagagagg
10441 actgggcacg gtgactacac ctataMtccc agcactttag aaggcccagg caggcagatc
10501 acctgaggtc aggagtttgt agaccagcct ggccagcatg gcaaaaccct gtctacacca
10561 aaaatacaaa aattaSctgg atctggtggc atgtgcctgg agtcccagtt tcttgggagg
10621 ctgaggcggg agaatcactt gaactcagga ggcagaggtg gcagtgagca gagattgcac
10681 cactgcactc cagcctgggt gacagagcaa gactcgcctc aaaataaaag aaggaaaaga
10741 ggccaggtgt ggtggctcat gcatgtaatc tcagcacttt gggaggctgg ggcaggagga
10801 tcgtttcagc cagggaggtc aatgctgcag tgaactgtga tcataccatc gcattcctac
10861 ctgggcaata gagtaagacc ttgtctcaaa aaaaggggag cggggaggaa aagagagtgc
10921 ccaggagtgg tgtgggaacc tgggttggag caggaaagat tggatagtcc aaagaaggga
10981 tgaaagtgat gggaatgtgg gttggagaag gggtgatggt ttaatgagag gtgtctgggc
11041 ctgctcacca cgctttctct gtttgggttt gtgatctccc gtcttcccca acaatgggct
11101 ggcctgggcc acactaccct gtgcacacct ggaaaaggta agcttccttt cagggctagc
11161 tttSggatgc agtcctaatc ttcatttttg cttttgttgg cagtgatgct ttcagctgtt
11221 caaaagagca atttagtctc tccctcRtgc tcctggaagc ttgtttagga tatgtttcct
11281 gtcaaagaag ttatttttca tgactctaat tttatgaaga aatctgacat taagaaaatc
11341 tcggtgcgga gagggtggtt ctcaggagtg gcagccatac agagagcagc tgcccgtgga
11401 gctgccgctt cctgctgttg tgttcaagag agagcagctg ccggcctgtc agcaccccag
11461 ggagtgaggc tcagggccag gctgcctccc tcactgcgtg ggtggccgag atcatgcaca
11521 ctgtggcggg aatagcagac tctgggggct gctacttgga catgctggca gttcccagct
11581 tatgtttcag caaagcttgg agagtcattg ctgggcttg'g taatggggac aacacagaac
11641 acttccctag cactgccgta tgtttgttgt tggtctttgt gcatctgatg gcctaagctg
11701 attaatttga ggcttgcatg aaaaaataat gccttgtgga ctttttcaag ggcactaggt
11761 gactgcttta aagaagaggt cagaggttct gtcctccaaa gctatctttg ggattcaaga
11821 agtgagagga aagaggcagt ggagacaaat gcccctcctt ttgtttgaag gctacccagg
11881 caactggtgg ctgcctgggg agagctggtg tcacatttca ttctgcagaa tgagaacctc
11941 ctgtaggctg gctctggtat tcactcattc ttgtctctca gtgtttgtca gagctttgct
12001 cctctcttcc caccttcagg acttttgcac atgtagcttt cattaccctt tggtaatgcc
12061 ctttagtaat ccatccctaa atagttcgtt tagatgaaag ggaatagtgt aagggggagt
12121 aaaagaaggc atgtctctat tgtagggtgg gtggttgata ttccttacag ttaactctta
12181 cacactgacc ggccccatga gggtggtgtt actgctgctt tctgactggt cttgtggaac
12241 ctggtagcca caaatggggg tccccacYgt gtgctgacat tgagaactgc gtggccgtgg
12301 gagaacagct ctcggttctg tggtgtgctg aatagacgac accctccatc tttattttct
12361 ttattccttt aagtactttt ccctattttt ctattccaga tgaagtttag tccctccctc
12421 tgctctgcta ttatagtagc tgtaatgttg atttctacta aaaatgatat gatcaaacca
12481 gacttcatct tattttcctt aataaaaaaa ggagagaaag attatcattt tatttttgag
12541 ttggtagaat agcatgaatc ccaggatgta aatgttgatc attttaactt ttaaatgatc
12601 taaatgcctt taagtaattt tttgtcatag ttttatttat ttgtctaggc accgtggctc
12661 acagttgtaa tcccaacact ctggaggctg aggcaggtag attgcttgaa tcaatcaatt
12721 aacacttggt gcatttgata agaattggct gaccagggag ttgggtgtgt ctgaagaaca
12781 aagcgaggct tttctattca agatcagcat ttctaattaa taatagttgt gctgcctgct
12841 aatatttttc ttaatttttc atgcaacatc acatttaact tttatggcaa ctccatgaag
12901 tgggtactat aatcatcttc atgttactag tgggaaaact gaagctcaga tttttcaggg
12961 ctctagagca gggattggca aactttttct ataaaatacc agaaagtaaa tattttaggc
13021 tttttgggcc catcctttga acagtatgat gatgtggggt tggagtcttt ttttgtggag 13081 tccatgtctg ggatgggaaa gaaggggtaa gggaaagggg agagtgatgc agaagtgggg 13141 tggaagggtg gtggtgattt tgcctcagct tccctgagga ttgtgccctg gaggcagtgg 13201 ggacggatgc gggcgaaaga aacctcagaa agctcttgaa gtggggctgc ttcaagaatc 13261 ttgaagctca agatgactgg tgactgctca gtgaatcttt gttaccgctg acatttcatt 13321 cagattttcc cttatgttac tgctatttgt cactttgtct tcctttctcc attataaggg 13381 aaggtctgtg gagtactgat ctgtctcagg catctctgcc caccccacag tgtgcggtgt 13441 cttgcacaca gtaggtgctc aaacacagtt attgaacaca ggctgcattg acttatcacc 13501 ttcggtatcc gtgctgcgca gtgcaaccat cttctcccaa agaagtcagg attttttggt 13561 ctaaaatagt cacagttaac aaagatactt gggaaatcct gaaaatatca taaatttcag 13621 gagtaagcag ttggacaagt tgtggacttc tgttgagtat cttctgcaag atccactatg 13681 caacttttgc tgcctgtttt tctttattca ctccatttta ttaaacttag tttcctccaa 13741 acccactgga agcaagaaaa gcaaacaaac aaaaccttag gttgctcatc tccattgttg 13801 catagtaggt gttgaatgga tacctactga gctagttcat ttctgatact aatttgatta 13861 cttctcctga acattctttc ttcttacaga actctctgtc ctctacctcc caaatcccac 13921 aaaacatctc gcctccacac ataggctgat tgcttgggaa tggtacagga ggcccttggt 13981 gattctaatt gatgaaagac taaaaaattc ccttataggg gaacataaat caatgtcaat 14041 ctgagattga gtaatacatc aaattagtat cagcaatgaa ctagtttcag caggctcttt 14101 acatactctt gcaggtatag gtgctgaggc tgaagctgta agctcagaga ttttgacata 14161 ctctttccct cctttccttc tttccttcat cagttactga acatttgtta tgtgccaggc 14221 attgggttaa gttcaaaaaa atatacagtg tctgatttca aatacattat tccactctag 14281 tggcaagaga aaaggaagta gtggccacat gatatgaggt gtgagcagct ggtaagaacc 14341 tctcctggcc taggaggtaa gacctcctca aggggtttgc tgccaaactc ctgaatgcga 14401 tcaagagtaa ttaaggtaac tgtgcagctc tggatgaatt aacaggttct gcgcttgact 14461 ttctccattt gtgcaggtcg gagatgtccg cccagaggtt aatttctaac agaacctccc 14521 agcaatcggc atctaattct gattacacct gggaatatga atattatgag attggaccag 14581 tttcctttga aggactgaag gctcataaat gtaagtttta tacaattcct cattgaaagc 14641 ataattgtat ttctcttaac ctgtgaaatc ccoaaggatt acttctcctg agcattcttt 14701 cttctttaca gaactctctg tcctctacct cccaaattct acaaaacatc tcgcctccac 14761 acataggctg attgctcagg aatggtacag gagggccttg gtgattctaa ttgatgaaag 14821 actaaaaaat tcccatagta ggagaacatg aatcaatgtc aagctgagat tgagaatttt 14881 taaaaagcat ttgctttttt agctcctcca aactgtgaat gtttgaacta tcaaaaatat 14941 gtataaaaat aggatctgga actgttattt gaagaacctt cttttgtaac acagcagcat 15001 cttttcatcc cagttggttt cctggagctg tggagagcag tgtatccatg tgtgctcata 15061 atgatggacc agaatagcct gcgatcattt ctaacactgt gttctaggca tgaattgtgt 15121 ttcatgctat tggagtaatt aagggtccag cacagagcaa ggaaatgtaa ttctgtgaga 15181 cgttgctact caactccagt cataactgaa tgctgactgg ccagcatgga acatccttgc 15241 ttcattaggt agagttaaca gttaacatgg taggcaggaa ctgctgccca gtctcagatc 15301 ctgtcttggt aaccccgtaa atatccctga aatgttcact tttgtcagag tgaattatcc 15361 acagcactgt aaaattttta tgctcttaac tggtccaggt tagtgggaca cattaggaaa 15421 tataagaggg cttagaatca gagaaacaaa tgtagcaaag aaagtgtttt tgttccttta 15481 atYatttcct ggctgcttct atcctgacct gtcttggcat tcatgatctc ttgcaccaac 15541 ttggtgctct tttgcaagaa gggctttcta tttttgttcc tttagcaaaa atcattaatt 15601 aaaccatatg tcctttaccc cttaagtgag tagtaataat taaatacttt cattcattcc 15661 tttattgaca tctgctcagt atttctttgt ggaaaaaaag cacatggcat ttatcaaggt 15721 atgctgtcat tgattatctg agagaaggct gttgggtgtg aaatcacctt ctttaattta 15781 cttaaattct ttaatatgtt gtttttctag gataaccttt atgtaaggac aattgcttgt 15841 tctgaattat agtggatatc aaaatgactg ggaaaattgc cacatatttt cttgaaatgt 15901 gggcttttac tgcttggtga catcaacttt acttttgtgt cagcttgctc ctatctcttt 15961 tccttagttt aagaagcaca gaagacaggc ctgctgctgt aattttggtt ggtacatttc 16021 cagagacaat tgaataaaat attttttgag gatattacat tctagaaata attatagctg 16081 gaaaacttca tgagaaacat aggcagaggt gtgactcatt ccttacttac ttactttacc 16141 tactatacca ttaataacaa ttgcttttgt taattttaat attcacgtca agataaaaca 16201 aaaatgcctg agcacttact gttctgatat aagagagctg ataagtagca gcccttagct 16261 tcaggaacct gaaaatatat gcgacacatt gagaacaaaa aataaacaaa cacaaaaccc 16321 cagatataca ggaaagtgaa aagatcaagt caactgttga aatgtgtgct ctggtacagt 16381 ctctttcatt taaaaatgct gtgactctga ccttcaagta tgactccttg ttttgctggg 16441 caaaattcaa agctaattag atgcagattc aaaagaacag aagcaggaca tttcaagggg 16501 aagcactcat tgcggacagg aatgaccata acagttatct ttatttgcot tacctgtaat 16561 ggatcacact tggcaacaat agctccatct ttagactRag ttcctctgct gaaattaaga 16621 ttcctacctt caaagggtgt ttcatctgag aagcaacctc agatggtgct tgagaattct 16681 tttgccatca gttttgaatc caatattaaa ccttatcatg tatcagaagt ggacatgtat 16741 ctgatacaga tttggcacca acaaagtgga aacctattta tttattcatt cattcaatat 16801 atatttattg agcacctgtt atgtgcagta ctattttaag cactggagag acaacagtga 16861 actgaaaaga cagaaatatt tccttgtata gagcttgcat tctagtggga gagacagaca 16921 ataaatagtt aaatagataa tgtcagatgg caatataaaa ggataaatag agtgggggta 16981 aagggatgtg gagtgtggag gcgacagtat agaattgcca tcgaaagcct tactgagaag 17041 gagagatttg ataaaagacc tgaaggaagt gagagattga gctataatat atctggggga 17101 agaacatccc agaaaaggat ccttggcggg agtgaactgg atcatgcaag gaggccagcg 17161 tggtaccagg ggagtgataa gaggcagaac agcaggaaac gaagtcaaag aggctgtggg 17221 gacaaggaca tgggggggcc ttagaggtca tgctaaggac tttgtttggc cttttactct 17281 gagaagtcac tggagggtct ggagcagagg agtgccatct ggcttaggtt tcaacagatc 17341 actctgcaac tgcggagtga aaggcatgag attagaagca gagaccattc aggaggcttt 17401 tgccatagtc caggcagctg atgatggtgg cttggagcca ggtagggaga gtgaagtggt 17461 gagaaatgca tacattctgg atatttttaa aagatggatc tggcaagatt tgtcaatgga 17521 ttggcggtat gtgtaagaga aagaagagtc aagaatgatt tgaagattta tgacctgagt 17581 aactggcagg atggaattgc ctttaactga gagggggaag agtatgggag aaggaggttt 17641 cagagaggaa ccccaggagc tctgttttgg acctgttaaa ttgagacatc tatcacatat 17701 ccaggaggac aagggaggag aggtgtcggg ggaagccgta gacttgagag tcatcagcat 17761 gtaaacggta tgcgttcaaa gccaagaggc tggataagtg agtgtagaca gaaaactgaa 17821 gagtccaatg ctgagccctg ggcacgcttg tgtttagaag ttggagggtt gcatagcagc 17881 ataggtgcct gagaggaatg tacagaaagt aggaagaaaa ctggacaatt gtggtttttt 17941 ggacgccaac tgaaaaaagg gttgcaaaga cagtctgtgt caaatgctgc tattaggtca 18001 agttaattga agactgagat ttgaccattg gatttataat gtagaagtta ctggcaatga 18061 tttgaaaagc agttttagtg gaatgctggg ggtaaaaggt tgatggaatt ggttcaagaa 18121 aaaatgagag gagaggaatt ggggacagca tgtacagaca aatctctcaa gtgtctcact 18181 ataaagggaa ggagaggaat gggtcattta tgaagagtgt ggctgggtga gacagggtgt 18241 tcttaaagca tcagaagcaa aagctgatga gtcaatgagt ggacaatcac attttagcag 18301 gaccactctg gccgctgtgt tgagaaaaga ctaaaggaat agcctcttaa tttgagctat 18361 cagtggtggc caaattcatg ctttttcgtg tgccaccttg cagataccag acccatgcat 18421 ggatgggtca gcaagaagcc aggtcattag gatgagaaac agctagagtt tgcaaaggct 18481 tggagtaaga tagtagagac aagtgtggga acatcactgt tggctctttg ccaggaattc 18541 atatcacaga attggcccta ctgccttagg aacaaaaagt ggtgtgagga aagtgtcatg 18601 aacctctctt tcaagtgaag tattaaccct tgtttttcat ataaccac'ga agtggaaagt 18661 tcaccattta ttctcaggag gtggagagga gggtgagtcg gatggagtag ttaacctagt 18721 cctaccctag attgggaagg aaaaaaatca tgtcaacttt cagctctctt gttccattcc 18781 attcattaaa ggttaggtta aggggggtta ctttattctg acgcattcca tttgcaattc 18841 ccctgggcaa ttgttctacc cctttacaat agtagtctct aagtagctca gtgtctagac 18901 aggacagagt gtggtagctt ggcKgtggtg gaaactcgtt tggaaggctt cttttgactt 18961 tcttaaccat ctagcctcat tggagctttg ctctgtataa agctttattc tgacttcttc 19021 ccagaaaagg atgcttttgg gaagacaacg ggctccactt ttcccatctt actgtagtgc 19081 ctatgcccat gaaacttaca tgtgaataaa acttcaggag agtttggata tttctgggat 19141 gagatgggga tctctgatgt ttcttgtaag aggctcaggg tgagaaaatc ccctggggat 19201 gggtcatagg gcagaagatg aggttggggg aaattagtgg aaataacttg aggaagctaa 19261 attttgttca aagggaaatg tgaataagca gagctactta aagcatattg ccatgaaaaa 19321 catagatgcc gcattattaa ggaaatgttt ggaaatgctg ttggatcatg aggattctca 19381 gattYtaccc acagcctgcc tctccccaac tctgccgcca cctgccttct ctgaaggctg 19441 taaccctccc atgcttcaca ttattgctgc tttttgatta tctccctggg ggtacttaga 19501 agggcagagg gaagggggac tgggctgggt tgagggcacg ctttcctcca cctctgggag 19561 tcagtccacc atttgcagtg tgaaagctgt ccttaggaga acaccttcct aRtacacaga 19621 tttgRaaaat gtcaatcaac atgattcaat tctaaaccga agacttaaat atagcattgg 19681 tgtataattt tagaatttgc ttttgctatg caggcagttg ataaagtaca gtgtagggaa 19741 ctgttattta tctgtacaat gtttcttcac agtccatgcc tagatagttg agatttaatt 19801 gtttcatgtt agttgtacct cagtacatgt ggtgtttggt atttgtcctt acatcttttt 19861 ttttttttga gacggagtct tgctctgtca cccaggctgc agtgctgtgg cgcgatcttg 19921 gctcactgca agctccacct gccgggttca cgccattctc ctgcctcagc ctcccgagta 19981 gctgggacta caggcgcctg ccaccacacc cggctaattt tttttttttt tttttttgta 20041 tttttagtag agacggggtt tcactgtctt agccaggatg gttttgatct cctgacctcg 20101 tgatctgccc gcctcggcct cccaaagtgc tgggattaca ggcgtgagcc actgcgcctg 20161 gcccttatgt ctttttttat gcctcatttc tactatatta aaaggcaaag tatacaattt 20221 ctatttcttt ggcacttaag gttagacaca ttagtttttc agagtgctga atacacattg 20281 ttaacctcta ggcaatagta tatgtaggat gaaagtatga attatgcaga gtaaattgtt 20341 aatctgattt cttcactttg gaaaaagtgc tacatataca taatttaatt agaataaagc 20401 tttgcattta aacacattta atatttaagc tctaatcact tatttttaga aaattgctaa 20461 gcatcctcat tgtatttcca catagacaag cttcttagtg taggccaaaa aatgtagaat 20521 gattcaactc tgttgtgtcc tagatttgag gccttattgt gcaagtacca agttctgtag 20581 acaatccaat ctaaccctgg tcctagagat aaagaattgt gattgttatc caagatagga 20641 ccaaggagag ccctgtttag ggaattacag acaaaaatgt tattgggttc aaaggaggga 20701 gacaacacat caattatgga aattagttga ggcttagtag atagtgtagc ggatggactt 20761 ggaaggatgg atacactttc attagctcca cgttgggaaa agttgttcca gccaggtagt 20821 ggtaagagcc ctggtaaggt gtggctgtga ggaagctgtg gctaaggtaa atacagggac 20881 acagtgtccc ctagtttgat tggaaacaca tggcttttaa agtggtaaat tgggagaaaa 20941 tgacaattct aatgacagta gccagcacct gtggtgtact gttttctcag atgtcagaca 21001 ttgtataaag tgctttatgt gtggaatctc ttttactctg gaaaagtggg tcagttctgt
21061 ttttgttgga gagcttttca tgaaagagca atatcatcag ggctgtgatt aaagcaggtt
21121 tgtacagaaa taaagcacag ggaaaggcag aaggcagaga gagcactcaa gaggccatct
21181 gtatgttctc tgcaagaagt aatgagagcc tgaattatcc atggtgaacg taagtgagaa
21241 tgcaaagttg ctacctaatt gagatgctaa ccaattgagt tgttaaacta aaatttggcc
21301 caagaaggcc tccattattt gcatacttgc attcttaggt atgagctgtg acctagctta
21361 ctaggtaagc aaactgaaaa cctagccaat gcttctgtaa caatagctcR gtctcagcta
21421 atcacagcag ctacacttca ggcactccca ggcagacagc tgttcaaatg gtgttcaaat
21481 ccagctgttt ccgtaccttg cttctttttt ctgtacatca ttttcctttt tctgtcccta
21541 aatcttaacc aaccatgagg cagcccagag tctctctgaa tcKat±ctga ttctgggaRc
21601 ttcccggttc aagaattttg ttttccttgc acagttaata tctgttaaat ttaatttgtc
21661 taaagttttt cttttaacag taggaatcca aatgcactta aaacttgagt tgatagtctg
21721 ctaaaaattt tattttctaa ctcaaaacac attagtggaa tcttgaggtc ctagataaYc
21781 tatgctctRg tctaggttcc ttccttgaga tatggaaagc atgagtcaat gaggcctaaa
21841 tcccttcatg ctatctgaga cagaaacctg gaggcggagc agactcttcc ctcctcctca
21901 tcctttacat cttaYcagtc tccagccaac tgcggatcag actgttgact tcctttcatg
21961 tttcatgtac caaagcctct ccaaaaagct ctgattcttg aatgttcaag ttttaaacta
22021 ctgtgaaata ctaatcccag aattaactgt ctttcagatt ccattgtgat tggattttgg
22081 gttggtcttg cagtcttcgt gatttttatg ttttttgtgc tgaccttgct gaccaagaca
22141 ggagccccac accaagagta agtttgggct gttcctaaat gtctcttaag cctcacagga
22201 gactgaggaa atagaaacta ctacccaggg ccggaggtgc ttcccgtgga tgaagagaag
22261 ggggtggtta tagatttgct gtctggaccc agatgcccgt ggatgggatc cagctattct
22321 cctggctttt tgtttcttgc gtcttgggct tattttcaag tggactttta tgaaattggg
22381 tttcttggtc ctttcctaca gtcttaMtga tgatgtgggc ctgattttta cattgaaatg
22441 tttattaatt acaatgtatt ttattcatat tcccaaaagg atttagatg'a ttcaccatat
22501 taaaaacagt ggaaaagaca atggcaaaat aggatgaaaa ccagttttaa aaattatttc
22561 aagaagcaga gtaatagttg ttcctggata ctcaactcaa tttaactatt gacaatgatt
22621 aataaattta acagagtttt cttaaaacta ggatacaaag gcaaacactg tggtttacat
22681 aattctcata acatggtagg acttgtgatg atgctggact ttggattgat ggaggaaagt
22741 attacatgag tgcttaaaga acattgagaa acatcaaaaa gtatttgttc agtaatggct
22801 tgtaaaaaat tcagaaatat attaaaggat ttatatattt tataccaaat ttttaataaa
22861 agggaaaata gagaatattg aatacctcat tagtcagcac cagcaggcaa cagtaattaa
22921 aagagttttt ggccggtatg gtggctcacg cctgtaatcc cagcactttg ggaggccaag
22981 gcaggcagat cacttgagtc caggagttca agacctgcct cgctatggtg aaaccccgtc
23041 tctattaaaa atacaaaaaa ttagcctgtt gtggtggagg gcacctatag tcctagctac
23101 ctgggatgcW gaagcacaag aatcacttga acctgggagg tggaggttgc agtgagccaa
23161 ggtcacacca ccgcactcca gcctgggcaa cagagccaga ctctgtctaa aaaaaaRaaa
23221 aagaaaaaaa gtttttgata aaggggctgt gcggaagtta tggacaggga agagggcaac
23281 tacgcaggct catgcaggac agcagggctg gcaacactga ggagcagcca tcctagatcc
23341 caggaagcaa ggggtggggc cacagctgga accctggagt ctgctgagag gagttgtgac
23401 tttgggctga gggacacagc taatccagtg tgacctctca gggaaggagc ctcaaagtta
23461 agaccaagac ctcagtcttc tccctccttc ccatttctca ccattgctcc ccactggctg
23521 aacacaacca gcagccagag gctgcatcaa aggctagttt cccaagcaca gagcaggaca
23581 ggggagggtg ggagagacct gggagagtga atataagttt cacaggaatc ctggcatttt
23641 ggttttgttt tgtttttttg tttgtttttt gagacggagt cttgctctgt cacccaggct
23701 ggagtgcagt ggcgccatct tggctcactg caagctccgc ctcccgggtt cacgccattc
23761 tcctgcctca gcctccccag tagctgggac tacaggcacc caccaccacg cccagctact
23821 tttttgtatt tttagtagag acagggtttc accatgttag ccaggatggt ctcgatctgc
23881 tgacttcgtg atccgcccgc cttggcctcc caaagtgctg ggattacagg cgtgagccac
23941 cgcgccgggc cgaatcctgg catttttata ttcagtatct catttttgcc tcacagcctg
24001 gcagcagctc tctttcatgg ttgtgtctcg tcacagcctc cggcatctgt tgttcactct
24061 tcagcctgca cttgaactat aacaaatgcc tgtgcaaagg gaacagttct tgccatcact
24121 cagctatttc tagatatcag gggctaggaa gaaaaaaatg taaacacagc caggcacggt
24181 ggctcatgcc cgtaatccca acagttctgg aggtcgaagt gagaggattg cttgagctga
24241 ggagttcgag aacagcctgg gcaacatggc aaaaccctgt gtctacaaaa aatacaaaaa
24301 tcagccgggc atggtggtgc acacctgtag tcccagctac tcaggaggct aaggtgggag
24361 gatcacttga acccaggaag tcaaggctgc agtgagctgt gattgtgctg ctgcactgta
24421 gcctaaatga cagagtaaga- ccctgtctca aaaaaaaaaa aaatttaaac acaaacattt
24481 taaatgaaag gaaaatatca tttttattga agcatttgga gacatatggc tgagaatcta
24541 tttaacaacc actaaaaaga cacaagcttt cctttttctc tcttcatgtc attgtaaata
24601 ttcgcacatg cttcacttct attatattgg cccagcgaat ttgaaaatat ggtgtgggtt
24661 acaagagggt taagaaaagc ctagaataaa ctcagattaa ggcacaactc Rtagagtggt
24721 cagggaat.aa taataccctg ttaatcttcc ttttcctcag aagaaccaat ggagttttgt
24781 gggttattga gatagatgtg tgtgatgtag aagacaggag attttaggac taggaacact
24841 agcatgaaat agaatgtgac ttcctgtgat ggttaatttt atgtgttaac aattctagat
24901 gtttctgtga aggtatttct tagttgagMg taccatttat cagtactctt tggattaagc 24961 agattaccct ccatgctgtg agtgggcctc atccagtcag ttgaaggcct taagagaaca 25021 aagactgacc tcctcaagcc aaaagggatt ctaccaggac actgcctttg gactgtctcc 25081 accctgccag cctactctac atatttggga cttgctggtc cccaaagtta catgaggcaa 25141 ttccttaaaa taaaactctc tctttataaa cacacacaca cacacacaca cacacacaca 25201 cacattgtgt tSgttctgtt ttctctggag atccctgact aatcttcact tccgtgtggt 25261 gtcatggcaa ccctgggtag ttgagacttg ccttttagcc ttacagttgg caattccctt 25321 ttgccactag acagtctgtc tttgatgatt attctgaaca atgctcaatc acagaacagg 25381 ggtcatttta accctgaatg cttctaacaa ggttaaggaa aaataacttt ttataacatt 25441 taagaaaata atggatgttc cctcagggcc atattttaaa tgcttctaag cacaatMtga 25501 cacagtataa gatatggcat ttggttgcag tatatcataa tagaaaactg aaagaatcag 25561 aacatgtgga aaaacattga aataaatctg ttataaaggc ctttaggtct taacaaactt 25621 gacatttgtt tggcaggagt ttccttctta ggtggacatt gttttttcca tatagccaat 25681 gttaatagaa aacaactaat tttaagtttg cctatgataa ttagggggtg aggggaagga 25741 cgatacaaca ttgaaaataa taagagccat tttttttgat tccagggtca actaattact 25801 ttatatctca ccaagtcctc acaggaagat gaagtaaatt ccatgtgttc ccacaataca 25861 gataagaaac atgaggctga gacacttgcc taaggcttca caggttaaag aaaaaatgta 25921 gagtcaaacc tcaaactaaa gtctcttcgg tgctgaagtt cctgtcatta accactacac 25981 tcaactatct cagattggca atttttaatc tcttccttct attgaaccgg agatgttagc 26041 aagcaaccag atttgttatt ttttccataa attattgggg tacaggtggt atttggttac 26101 ataaggaagt tccttagtgg tgatttgtga aatattggtg cacccatcac ccgagcagta 26161 tatgctacac catRtttgta gtcctttctc cctcaccccc tccttctcgt cacccccaag 26221 cccccacagt ccattgtttc attcttctgc ctttgtatcc tcatagctta gctcccacgt 26281 atcagtgaga acataagatg tttgcttttc aatttctgaa ttacttcact tagaataaca 26341 gtctccagtc ccatccagat tgctgcaaat gctgttaatt cattcctttt tatggctgag 26401 tagtgtccca ccatatatat 'cacagtttct ttatccactt gttgattgat gggcatttgg 26461 gttggttcca caattttgca attgtgaatt gtgctgctat aagcatgtgt ctgcaagtat 26521 cttttttgta taatgacttc ttttcttctg ggtagatacc tagtagtggg attgctggat 26581 caaatggtag acctactttt agttctttaa gaaatctcca cactgtttcc catagtggct 26641 gtactagttt acattctaca ccagcagtgt agaagtgttc cctgatcacc acatccatgc 26701 taacatctac tgttttttga ttttttgatt atggccattc ttgcaggagt aaggtgatat 26761 cgcactgtgg ctttgatttg catttccctg atcactagtg atgttaagca ttttttcata 26821 tatctgttgg ccatttgtat atcttctttt gagaattgtc tattcatgtt tttagcccac 26881 ttttggatgg aattgttttt tttttttttt ttcttactga tttgtttgag ttcgttgtag 26941 attctggata ttagtccttt gtcagatgta tggattgtga agattttctc ccactgtgtt 27001 ggttgtctgt ttattctgct gactgttcct attgctatgc aaaagctttt tagtttaatt 27061 aagttccaac tatttatctt tgtctttatt gcatttgctt ttgggttctt gctcatgaaa 27121 tccttgccta agccagcatc tagaagcgtt tttccaatgt tatcttctag aatttttaca 27181 gtttcaggtc ttaaatttaa gtccttaatc catcttgagt tgatttttgt ataaggtgag 27241 agataaggat ccagtttcat tcttctacct gtggctagcc aattatccca gcgccatttg 27301 ttcaaaagag tgtcctttcc ccactttatg tttttgtttg cttttttgaa gatcagttga 27361 ctgtaagtgg gtttatttct gggttctctg ttctgtttca ttggcctatg tgcccattta 27421 tatactagta ccacactgtt ttggtgacta tggccttata gtatagtttg aaaccagata 27481 gtatgatccc tccagatttg ttctttttgc ttagtgttgc tttggttatg caagctcttt 27541 tttgttccat atgaatttta gaattatttt tgctaattct gtgaagaatg atggtggtat 27601 tttgatggag attgcattga atttgtagat tgcatttggc agtatagtca tttttacaat 27661 attgatttta tccatccatg agcatgggat gtgttttcat gtgtttgttt tatctatgat 27721 ttctttcagc agtgttttgt agttttcctt gtagaggtct tttgactcct ttgttaggtt 27781 attcctaagt ttgttttttt gttttttgtt tttttgtgtg tgtttttttg cagatattgt 27841 aaaaggggat taagttcttg atttgattct ccgcttggtc gctgttggtg tatagaagag 27901 ctactgattt gtgtacatta atctagtgtc tggaaagttt gctgaattct tttttttttt 27961 tttttttttt tttgagacgc acactaccat gcctggctaa ttttttgtat tttagtagag 28021 atggggtttc actgtgttgc ccaggctggt ctcgaactcc tgagctcagg caatccaccc 28081 acctcagcct cccaaagtgc taggattata ggcatgagcc actgtgcccg gcctgctgaa 28141 ttcttttatc agttctagga gctttctgga ggagScttta gggtttgctg atgatcatat 28201 cgtcagcaaa cagtgacagt gtgacttcct ctttaccgat ttggatgccc tttatttctt 28261 tctcttgtct gattgctctg gctaggactt ccagtactat gttgaagagg Mgtggtggga 28321 gtaggcatcc ttgtcttgtt ccagttctcg gagggaactt ttcagctttt ccccactcag 28381 tattatgttg gctgtgggtt tgtcatagat ggcttttgtt acactgtatg tcccttgtat 28441 tctgattttg ctgagtttta atcataaaga gatgctggat tttgtcaaat gcactttctg 28501 catctattga gatgatcatg tatttttgtt ttttattctg ttaatgtgKt gtatcacatt 28561 tgttgacttg catatgttaa accatccctg catccctggt atgaaaccca cttgatcatg 28621 gtggattacc tttttgatat gttgttggac tcagttagct agtattttga taaggatttt 28681 agcatctatg ttcatcaacg atatcggtct Ktagttttct tttttggtta tgtcctttcc 28741 tggttttagt attggggtga tgctggcttc atagaataaa ttaaggaggg ttccttcttt 28801 ctctatcttg tggaatagtg tcaaaaggat tggtaccaat tcttctttga atgtctggta 28861 gaattctgct gtgaatctgt ctggttctgg acttttttta ttggtaattt ttaaattacc 28921 attttaatct cgctgcttgt tattggtctg ttcaggatat ctagttcttc ctgatttaag
28981 ctaggagggt tgtatttttc caggaattta tccatctctt ctaagttttc tagtttatgt
29041 gcgtaaaggt tcatagtagc tttggatgat cttttgtatt tcagtggtgt catttgtaat
29101 aactcctgtt tcattttttt aatgaggtta tttggatttt ctctcttctt ttcttggtta
29161 ttcttgctaa tggtttatca attttattta tcttttcaaa gagccagctt tatgtttcat
29221 ttatcttttg tatttttttg gtttcaattt catttagttc tgctctgatg atcttggtta
29281 ttacctttct tctgctgggt ttgggtttgg tttgttcttt tttctctagt tccttgagat
29341 gtgaccttag actgtcagtt tgtgctcttt cagtcttttc gatgtaggtg tttagggtta
29401 tgaactttcc tcttagcact gcctttgctg tatcccagaa gttttgatag gttgtgtcat
29461 tattgtgttt cagtttgaag aatttttaaa tttctatgtt gatttcattt ttgacccaat
29521 gctcattcaa gagcaggtga tttaatttcc atgtatttgc atggttttga aggttccttt
29581 tggagttgat ttctagttct attctactgt gatctgaaag agtacctgat ataatttcag
29641 ttttcttaaa tttattgagg cttgtcttat ggcctatcat aagatctatg ttggagaaag
29701 ttccatccac tgttgaatag aatgtgtatt ttgtggttgt tggatgaaat gttctgaata
29761 tatttgtaaa gtccatttgt ttaaatccat tgtttaaatc cattgtttct ttgttggctt
29821 tctgtctgat gagctgccta gtgctctccc tggaggattg aagtccccca cttagttcta
29881 taagtaattg ttttataaat ttgggagctc cagtgttagg tgcatatatg tttaggattg
29941 tgatattttc ctgttggaca aggcctttac cattacataa tatccctctt tgtctctttt
30001 aactgctgtt gctttaaagt ttgttttgtc tgatacaaga atagctaccc ctgcttgttt
30061 tggtgtccat ttgcatgaaa tgcctttttc caccccttta ctttaagttt ttgtgagtcc
30121 ttacatgtta ggtgagtctc ctgaagacag caggtagttg gttggtgggt tcttatccat
30181 tctgtggttc tgtatttttt taagtggagc atttaggcca tttacattca atgttagtat
30241 tggataagag gtaccattgc attcatggtg ctctttgctg cctgtgtact ttggttttat
30301 tgtttttgct ttttaactgg tatttttgtt tcataggtcc tgtgtgattt atgctttaaa
30361 gaSgttctgt tttgatgtgt ttccaggatt tgtttcaaga cttaaagctc cttttagcag
30421 ttcttgtagt ggtggcttgg ttgtggcgaa ttctctcagc atttgtttgt ctgaaaaaga
30481 ctgtatcttt ccttcatata tgatgcttag tttcactgga taaataattc ttggctggta
30541 attgtttggt ttgaggaggc tgaagatagg gccccaatcc cttctagcta gtagttttct
30601 gctgagaaat ctgttgttaa tctgataggt ttttccttta taggttacct aatgcttctg
30661 tctcacagct cttaagattc tttcctttgt cttaactttg gataacctga tgacagtgtg
30721 tctaggcaat gatctttttg agatgaattt cctaggtgtt ctttgtgctt cttgtatttg
30781 gatgtctagg tctctaggaa ggctggggaa gttttccttg attatttccc caaatgtgtt
30841 ttccaagctt ttagaattct cttcttcctc aggaacacca attatcctta ggtttcattg
30901 tttaacataa tcccagactt cttggaggct tctttcRtat tttcttattc tttttcaatg
30961 tgttggattg ggttaattcg aagaccttgt cttcgagctc tgaatttctt tcttctactt
31021 gttcaattct attgctcaga ctttccagag cattttccat ttctataagt gtgtccaatg
31081 tttcctgaag ttttgttttt cctttaaact atctatttcc ttgaatattt ctctctttac
31141 ttcttgtata gttttttaga cttctagtgt tgggctttgc ctttctttgt tgcctccctg
31201 attagcttaa taactaacct cccaaattct ttttcaggta aatcaggaat ttcttcttgg
31261 tttgcatcca ttgctggtga actagtatga tttttagggg gtgttaaaga gccttgtttt
31321 gtcatattac cagaattagt tttctggttt cttctcattt gggtaggctc tgtcagaggg
31381 aaactctagg gctgaaggct gttattcata tacttttgtc ccatggagtg ttcccttggt
31441 gtagtactct cccccttttc ctatggatgt ggcttcctgt aagctgaact gcagtgattg
31501 ttgtccctct tctgggtcta gccacccagc aagtctacct ggctctgggc tggtactggg
31561 ggttgtctgc acagagtcct gtgacgagaa tcgtttatgg gtctctcagc catggatacc
31621 agtgcctgtt ccagtggagg tggtagggag gggcaatgga ctccgtgagg gtttttagct
31681 ttggtggttt aatgctctgt ttttgtgctg gttggcctcc tgccgggtca tgttgctttc
31741 cagagagcat cagctgtggt agtttgggga ggaacaggtg gtgggcaggg ccctagaact
31801 cccaggagta tatgtccttt gtctttagct accacggtgg atagggaagg accatcgggt
31861 tggggcaagg ctaggYgtgt ctgagctcag tctctccttg ggtgggtctt gctgcagctg
31921 ctgtgggaga tgggggtgag gctcccaggt caatggagtt gtgtacctag gaggattatg
31981 gctgcctctg ctgagtcatg caggttgtca gggaagtgga gaaaagccag cagtcacagg
32041 actcacccag ctcccataca aatcgaaggg ccggtctcat tcccaccatg tccccactaa
32101 cagcccctaa tctgtttcca ggcaatgggc agccagactt gagaacttgc cccaggctac
32161 cctccccgca gctgcaaaag aaaagggctt ggttcattcc ccacctatgg agtctgcaca
32221 ctggattcgt gtccttcccc gaattctggc caggaggctt cttgccccat tcaaattgtt
32281 acaaagttca gctggagatt tccttctccc tgtggtgttt ctccccctga tcccctggct
32341 cctctggccg ccctgcctat atattcctat ggtgctaggc aggaatggcc tgcttgtgga
32401 cccagcgagc gcccagggcc tttcttcttc ttcctctacc cctgtatttc actcggctct
32461 ctaaattgac tcagctccag gtatggtcag aaacttctcc cacaaagaga ctttcagttt
32521 ctctagtggg gatgtgtgtt caggagagga ggctctccct ttcccacttc tgcagttggg
32581 gcacccacag tatttggggt atctcctggg tcctgcagga gcaatctgct tccttcagag
32641 ggtctgtggg tcctcttggg attgctggtt tgttcttgca gtggatctgg agctaaaatt
32701 cagtgtgagc ctctgcacac tgctctgtcc atccaagttg gagttgcaat ctagtcctgc
32761 ctcccatcca ccatgatgat tacttctgtt tatttgtttt tatcacatac tgtgccaagc
32821 acggggctct ggactcaaac atttccaggt ttacttattg agactttggc ctggattctt 32881 aacttctctg tgcttgagac tccttatata taaataaaga agtgtctgcc tcctgggatt
32941 attgtaaacc aagcttgtcc aacctgcggc ccagaatagc tttgaatgca gcccaacaca
33001 aattcgtaaa tttgcttaaa acattatgag atatttgtgt gtgtgtgtgt gtgttttttt
33061 ttttttttct catcagctat cattagtgtt agtgtatttt atgtgtggcc caagacaatt
33121 attcttcttc caatgtggtc cagggaagcc aaaagattgg acatccctgt tgtaaacaca
33181 gaagtgagca gactgtggag cttcttgcaa aagctgggct cagagcatcc ctcagtaaag
33241 atgagtcact gtgtgagctg aagagtctag gtaaagagta ggagggagaa gaaaaaggaa
33301 cccattcccc atgggatacc cagaccctaa gcgttatatc tacactttca aaatgttgcc
33361 ttattctatc ccagcttcac cttggctgtg tctcaaagtg aaacagtaag atgaatctag
33421 aatggcagct aggtagagaa tcctggtgat gggtcagaag gaccaacacc tttgaattct
33481 gattgcttta catatctggc tctctgggtg gtttgtcact aagacagtta cttcctaaag
33541 tagaacatca acagcatcct tttggaacat gattccaaca gcatggttaa gatacctgag
33601 taagtctgca agctcagaat ggactggtgc tggatctacc actgaattta atatttttct
33661 ccctggagac atctcaggcc agggccaaat ggctccctag gatctcataa ttgctgacac
33721 atggaagtgt gtgagcttcc ctttgatagt agtaatcaca ggatgacaga taacaccacc
33781 tgtgccctga ctccctgtgc ccgtgcccac aacagacatc agcaatggac tgcatcatct
33841 tccctgcata gccaagattt tgcctgagaa tcattttcaa aacagatctc caggcagcca
33901 ccaggaacca atcacagttt acctgagaga gaatgtttat ctgccatccc tggctggact
33961 ttcctttaat gtactggatg acaggagttg agatccattt aactgcacag ggccaatcac
34021 actgcaataa aatgggtatt tttttcccct gaattttttg acctgggatc atgctagaat
34081 gagaatttct ggaatcacta actacttagt ggcttctgtt aacgggggaa atatgtgcta
34141 ggctaggctg tcagacagta agaagttaat cctcaaatct tggtatgaca caacaaaggt
34201 ttatttcttg cttaaactcc atgtctgcta aggttggcaa ggggcagaca gaaacatctc
34261 cacttgcagc tgccctttcc ccaccacgcg gcctcctagg ttgctatgac acggtaagaa
34321 aacacttgga gatctcaagg ctgcccctcc attcttcagc cggaagcagc acacatccct
34381 tcctcccaca gctcattagt cagaactagc cacacccacc tgactgccca gggctgggaa
34441 gggtagtctt ctgtgtatcc agaaggaaag aggcaggtat tggtgagcac tgagaaggta
34501 gactgcagag gaagaaagac tgttataaag ctgacatcac ccttcttaaa agcttttagg
34561 cctaagaggc agctttgatg gggattattt tttccatgga atatttgctt ttgtatggtg
34621 gggcaggcag gggatctctt ttctggattt tttaaggaca gtgaagataa aagaagcatt
34681 tcctcctgct ttctgagcta aggaaccatt tactctgcat gctgtcttgt taaagcatta
34741 tggccccatt atcataaatc tgccagatag agagtaatgg gaaaagcttg ggtcacattt
34801 ttatgtagaa tacttcRgac ttcttgaaaa attatcaaac cccagacatt acgtgcctca
34861 cataccctcc ttttgtagcc tttgttaagt acatctcatc caggtggggc tgttcttcac
34921 ttaggggcca gtgcaggatt gctcaggacc ttgtaaaaat gggaattata ttctatcttg
34981 aatagcaatt tacctgaata tggcactcat taaagaatgg gtacatggaa gtaaagacta
35041 acaacaggga aatgtcattg ttcaaaagtt ttgatattga ccatggccac aggaactaga
35101 tggcaaaatg cagatgtagt gagtgattcc tgaggaagcc ctgtcgttct gaggtatcta
35161 ctgctatgaa agggtagaaa atctatttta tgtgtctggt tcagtctata acttttgttc
35221 aggagttatt tttttttttc ttttgagatg gggtctcact ctgtcaccca ggtggcagtg
35281 cagtggtgcg atctcggctc actgctagcc tcaactgccc aagctcaggt gatcctccca
35341 cttcagcctc ctgagtaact gggactgggt gtccactaca cccagctaat tttttttttt
35401 ttttttgaga cagagtctca ctctgtcacc ccggctgaag tgcagtggca cgatcttagc
35461 tgactgcaac ctccaccacc tgggttcaag tgattctcct gcctcagcct cctgagtagc
35521 taggattaca ggtgcccaca accatgccca gctgagtttt gtatttttag tagagatggg
35581 gtttctccat gttggccaag ctggtctgga actcctaccc tcaggtgatc cacctacctc
35641 tgcctcccaa agtgctggga ttacaggtgt gagccactgc acccagccta atttttgtat
35701 ttttagtaga gacagggttt caccacgttg cccaggctgg tcccaaactc ctggcctcaa
35761 gtgatccacG tgcctcggcc tcccaaagtg ctgggattac aaacgtgagc cactgcgcct
35821 ggccaggcat atgtatcttt aaaagcccac tggaagtgac tgctggaaat atcaggtgcc
35881 cacaggctga tctgcccacc acttttcatg agtggggcaa actgaagaca gttgtcagca
35941 tgctaagacc attggcacag cagcaggtat ttcatttgta aaatctcatc aaaaatctgg
36001 aaaattgcta gtggaataat ccagagcatt acttgtgttg tccctctgca aatggaagtg
36061 gaaggagtct atccttctgt cttgatttgt cttcagggtc acagaggtag gagcacttca
36121 ctaacaccag agaagctgtg cctctcatag gaatcagggc tttggacaag cccacagata
36181 tagctgttca gtacatggta tttcataaag tgctcactga ggtctaatta gtgcaaagct
36241 cttttagggt tgcaaagatg aatccaatta cagcacatca gtatatagaa gagaaagacc
36301 atacacaaaa tagccctgac acaaggcaga aaaggatgca tcctgtaaga gcaggttagt
36361 aaaatatggc aagagcacag aggagggaca gatttcttcc cattgggaga tcagagtgtc
36421 tccatggcat cagtgacatt ggagttgaca gtgaagaagg tattttattt tgacagacag
36481 actggtggaa gggcctatca ggtagaaaga acagcacaag cgaaggcaca gagagtggca
36541 gtcttgagta cattgggggc tgtcacatcc ttcgtggcta gactgtagga ttagcaaggg
36601 gactgaaact gaagggcttt tggcccttga aagaaggaaa ctgcctagct tatgtctatg
36661 gttatatgat tttctacctg aaatcttcca attcacaagc atgatctaca tctccatata
36721 cttaggtctt cttcaatttc cctttgtaat gttttgaaaa tttcagagta caggtctgga
36781 acattcttgg ttaaatgtat tgttaactat tctatttttt tgatgttatt gtgaatggag 36841 ttaccttttc attagtagca tgtagtaata tagtgatttt tgtataaggt caattgaaag
36901 aaaaatcttt ttaaatggca gtggtggtct aactggataa acatatgaaa aaaaaatgaa
36961 tgagattacc tcatctcaat atggagcgtg tcttcaaagt aaaagctcct agaactgcac
37021 tggctggtag aactttctgt gatgatggaa atgtgtctgt gctgtccagt tagatagcca
37081 ctggccatgt gtggttactg agcatttgca atatgggtaa tatgactaag aaaccaaatt
37141 ttgaacgtta cttaatttaa atttaaatag ccacatattg gtaatgtatc atattggcca
37201 acatggttag aagaaagcat aggagaatat cttcatgatg ttagggtagg caattgtgtc
37261 ttagagaaga tgtaaaaagc actaaacata aaggaaaaaa attgataaac aactgattaa
37321 aacccctgct catcaaaaga cacctttgag aaaatgaaaa tgccaagtca cagatcaaga
37381 gaaaatatat gcaatacaca taactgacaa agaactcata ttccgcatat attataaaac
37441 ttccacaagt caagaacaaa aaccaaataa ctcagttttt aaatagactc ctacagatgc
37501 ttcacaaaag aagataaacc aatagccaat aagcacagta agagatgctt agatgttatt
37561 acacatcaga gaaatgcaca ttaaagtcac agtgaaatgt catttcactc ccattggaat
37621 gtctaacatt aacatgattg acaacaccaa atgttgggaa gcaggttgct atggcttgga
37681 tagcttgttt gcccccacca aagctcatgt tgaaatttga tcccgtgtgg cagttttggg
37741 aggtggggcc taatgggagg tgtttgggtt ataggggtgg atcccttgtg aatggcttgg
37801 tgctattctc acagtagtga gttcttgctt tctcaagact ggattagctc tcttgggaat
37861 ggattagttc cctcaagagt aggctgctat aatgccagga cactcctcag attttccttc
37921 ctttttatgg gtctgcttct cctttgacct tctttgtagt gttgtgatgc agcatgaaag
37981 ccctaaccag aagccagggg catacccttg aacttctcag ctacataaac ttcttttatt
38041 tgtaaactac tcagtctcag gtatgctttt atagcaacat gaaacggact acaacagagg
38101 tggaaaaact aattctcaca cattgtggtg gagtgtaaaa tggtacaact actttggaaa
38161 accctttgca gtttctcata aagttaagca tagatccact acatgactca attctattct
38221 taggtattta ttcaagagca gtagaaacat atccacaaag agacttttca acaacgttca
38281 aagtagcttt actcacatag tcccaagcta gaaacaaccc aaatgtcctt cagcaggaga
38341 atgaataaat agattgtagt atgttcatac aatgggcatt agtaagaaaa aggacaaact
38401 attgtcatat gcaacaatat agctaaatct caaaaacatt gagcaaaaga acccaagcac
38461 aaaaggccat atatcatatg attccattta tataaatttc aagaacaggc taacctagtc
38521 tgtggaaata gaaatcagag cagtgttacc taaagggggt agaaattgaa tggaataaaa
38581 ctttctggaa tgaaggaaat aaatgtcctt tatgttgatt ggggtggtag ttacatgggt
38641 gtgttcatta attaaaattc atcatatttt gtacttaagg tttatgcatt tcacattgac
38701 atatatggta tgtaaacttt acccattgat gataagaatg gtaactgaat tgaagcaagt
38761 ggtgtccacg atgagttttt cacattgtca acccaaatta acaaacagag gctctctgaa
38821 agaaaatgat atttatttgg taatagggca ctgcagtggg aatactcatg tcatagtaaa
38881 ctatgtgtgt attctaggag gtaaaggaag ataaagatat tgaaaggaaa agtgaggagg
38941 attacacaat tatttcaaga taattatcct tggctacaag ggttaataac aaaggtggca
39001 tcagtcaaga ttggacagat agttgctggg tagatgtcct tgtagaagta tttttttgtt
39061 gtaagactga gatggccttt gtgcaagatt atggcttttg cagagtcttt tgtgatgatt
39121 cttattaaca ggcactcatg catgaggacc ctcgtttcat aaccttcccc agctctattg
39181 ccagggtttt ttttttgttt gtttgatttt tcttttgaga tggaatcttg cttggtcacc
39241 caggctggag tgcagtggca tggtctttgc tcaccacaag ctccgcctcc cgggttcacg
39301 ccattcttct gcctcagcct cccaagtagc tgggattaca ggtgcctgcc accacacccg
39361 gctaattatt ttttgtattt ttaatagaga cggggtttca ccattttagc caggatggtc
39421 tggatctcct gacttcgtga tccgcccgcc tcagcctccc aaagtgctgg gattacaggt 39481 gtgagccacc acgccctatt gccagggttt ttaacataag tgacttcatt ttgattctga 39541 caactttcac atttccctct tttgctgaag ctctttctca aaagtcatca ctgatgaatc 39601 atcccgtagt taaattttga ttgtcccttg gttctgggat ttacctgtac tggtttggtt 39661 taatctagtc ctacattaga gggaagttat tggtgactag gaatcagtgt caaaagcctt 39721 ttagccacat ttgagcaaca aaggtagttt ggagggagYg gctctcaggc taagtctacc 39781 tgaagtccat atttaagttc aattttgtct gttttgtagg tgttggctat cacttcaaag 39841 tgctggtcca gcattattct gttaggagtt gtacttctga agaattttaa caagtaacag 39901 gtacaaagtt tacaaacaaa agtagaaagt aaaattaata gtaatatgat aatctcagtt 39961 tgcctaacag ttttgaggca tgaacctggg cttaaaggca accaattaat aaatcaaatg
40021 aacacagggg attaggtgag acttgttgta actatgtgac ctgttttctt attttgtata 40081 tatgggtctt aattttccca gaggaatata tctaggtaca gtatgtagta ttagcaatag 40141 cacatatatt tatttactta aacaatagat aatatagagc aattttatta catggcatcc 40201 catgggtggg ttgaattaaa gtagagcaga cagagaacaa cagttgtatt agggatgttg 40261 acaaagtcac ccattgggtg cactaaagga tctcttaggt caggtttttt aaagttacca 40321 gcaaaacgta ttgattgtga aatttcaagt gcaccattat cctgtcaaat gaaaaaggta 40381 ggcattaaga ggggtcaaag tctcattatg atatggagtc tttttctgat gtcttgggaa 40441 aaactgtcta ctgcatgaag ctgtcaactt ctcatcctgg tttgcagttt gaatgtgact 40501 gtttatggca taaggtgtcg gtatgttttg attctgtgtc cctacccata tctcatctca 40561 agttgtaatc cccatgtgtc attgtcatgg gaggaacctg gtaggaggtg attggatcat 40621 gggggcagtt tcccctgtgc tattcttgtg atagtgagtt ctcacaagat gtgatggcct 40681 aaaagtctgt ggtgattcct tggctgggca cagtggctca tgcctataat cccagcagtt 40741 tgggaggccg aagcagatgg ataatttgag gtcaggagtt caaggccagc atggccaaca 40801 tggtgaaatc ctgtctctac taaaaataca aaaattagcc gagggtggtg acacatgctt 40861 gtaatcctag ttacttggga ggctgcggca ggaggatcac ttaaactggg aggcagaggt 40921 tgcagtgagc caagatcaag ccactgcact ccagcttggg tgacataaca agactccatc 40981 ttaaaaatga atgaatgaat gaatgaataa ataaataaaa gtatgtggtg ggttccccct 41041 gtcttgctgc catgtaagat gtgccttgct ttccctctgc cttccactaa aattgtaagt 41101 ttcctgagac ctccctagcc atgcagaact atgtgtcaat taaacctctt ttctttataa 41161 attacccaga ctcaggtagt tctttatagc agtgtgaaaa cagattaata caggtgcttt 41221 ggtgaactct ctgtgtgacc catacattag acattagact tgtcccttaa aattcaccaa 41281 gtttaaggtt atagggcttc agaaatagaa cagtttgcat ttttagtaat tctatggtag 41341 agagttggat tgaaagaatc tagaataatt caggatccag tctattctat aggcagataa 41401 caagaacttg aaaataatgc acagggctaa aaacctaata acaagagtgt tgtagttttt 41461 ctttagaaat ataacttttt ctctctcgta ttagtatact tttaatatta aagctcaatc 41521 tttagcaaag gcttatagat aattcccatc taattatagc cttcatgaac ctttcatgac 41581 ctgctcaaat cttcagtaac atgcttaagc ctttagtttt gtcccattct tcctctttct 41641 taaatgacca gtcattttac tttagaacaa aaatttacta cacagattct ttcttatacg 41701 aaattattct cttttctttt taaccttcct taccgaaagt gcatcttctt acttataact 41761 tcctccatgt ctctctctcc tgcttaccag tagagaaggg cttttgccat gttgcccagg 41821 ctgatctcga acttcagagc tcaaataatc ctcctgcctc ggccttccac agtgctgaga 41881 tcacagacgt gagccactgt gagatatgat gaggtttctc ttcaaatagc ttgatcaatc 41941 ctttattctt taattcatag tacccactcc cccacccctt tctccttttt gtcttttttt 42001 cctttctgcc tttgttacat gctaggacat gccacagtac caggcttatc agtacgagct 42061 cacattcctt tccttattta aaaaaaagac tagccctcta gctcatcaca gacacccctt 42121 ccccttttcc cctctctccc ttacgtgccc accttatcta aaaaaagttc aaatgtctaa 42181 ccaaccgaga ttaattcaga ttgtgcgacc cgaccccggc caatagaaaa agagtacaga 42241 gacaggactt gcgtcagaaa taaaggcttt catgcccctt tgttcaagtg tgctctcatg 42301 gcgactggca aaaaaacaaa caaacaaaaa tccctctaca caaaagtaaa attgcttttg 42361 ctaaaaatcc cttgtttgag tgttcaatct ccttaggatt ttgagcgtta ttcccaacag 42421 ccaccatgcc tggcctgttt acttttaaca attatacctg gattactcat agaaactgag 42481 atattagaca aagctagtca tcattttaag ttatttccct tttgatcatt tttatagcct 42541 gtgaatatca ggtgttcacc taagtaagaa tcttaaagtt aaatatatgg gtattttgcc 42601 aataactcag aagatacagc tgcttttatt aagccaacaa tatgaaatta gtcttacata 42661 tcaaagaatt acagaaacaa atttattctg tttttagggt gagtttataa Ytttatcacc 42721 ttaaaacatc tagtacaatc ttgatgaaca gtaaacccgg gcaaaattgt atgctgacaa 42781 ttttgaagac ctctctattt ttattttgtc aacaaactta aaaccagctt atttatcaaa 42841 aaattattta agtaatgtga actaaaaaca cgtgggttag ttactatatt tttgttacag 42901 tactcaattt aagtgctttt cttccctttt aaatttatta atcagatctc tttaatatat 42961 tttagtagaa aaatattata cacacacaac attaaaacat acagacaaca caaatacata 43021 gaagcagatt ctctcgcttt cgtctggata ttttagtcat gagacagtgt aacatagtaa 43081 tacaaactca gtttgtaaaa ggacagttga actcaaatta tatttctgac aaatgggacc 43141 tgttcacatg gctaatgttt atttgcccca atagataatc ttattaaggc tgtgaactaa 43201 aattttggat aaggaaattt tcatagcagt ttgataataa taataaKaaa cctctcatcc 43261 tttatttcag tttcaaatga gtttagggta tatttttaat atttatattt tagccaggac 43321 tggctaaatt gtataagaaa aataaaatct tcaaatggcc ttgaattttt agtaacaaat 43381 ctatcttttg tttgctggtc tggtttgctt gactagtcaa tgtgggtgag gaagcatttt 43441 aacaggtttt ttttccagct ttttctagtc cctccgtagt agataaagca atttttatgt 43501 cagacaaaga tatagtattc ctctgagctc aagattttga cttgtttgat ctgagaggct 43561 aacttttatg aacatttatg tagttatttg cttttaaaat accaatcctt taattaagtg 43621 ttccattacc caaagcactt tttagtcaga caaacctaga tttacatttc caaaggtgtc 43681 taaattgttg gttttcatga acctgttgta atctgtaaag ctgttaagtt ttttgcattt 43741 tgactggaat gccataagca gtgagtttgt cttaacacca gcagaaaagt ctgcagattc 43801 aaaataggca gaaaaataaa atagaggaga gagagcctag aaggctctat gtattaactc 43861 tatatttgca gtttctattt catctctaag ataaacaggc ttggggagtt tgaatgatct 43921 ccatgatgcc cattgagttt gtgtgcacca ggaaaagcca tgtggccagc tggagtccca 43981 gaagaccagg catgccttgg tatttgagaa tctcatttca tttctttatt ttattttatt 44041 ttattttatt ttattttatt ttattttatt ttattttatt tatttatttt attttacttt 44101 attttatttt attttatttt attttattat actttaagtt ttagggtaca tgtgcacaac 44161 ctgcaggttt gttatatatg tatacatgtg ccatgttggt gtgttgcacc cattaactca 44221 tcattttttt atttttttga gacagagtct cactctgtcc ccaggctgga gtgcagtggc 44281 gcgatctcgg ctcactgcaa gctccacctc ccaggttcac gccattctcc tgcctcagcc 44341 tcccaagtag ctgggactac aggcacccgc cactgtgcct ggctaagttt ttgtattttt 44401 agtagagaag gggtttcacc gtgttggcca ggatggtctc gatctcctga cctcgtgatc 44461 cgcccttctc ggcctcccaa agtgctggga ttacaggcgt gagacaccgc gcccggcccc 44521 ttcatttctt attaatttct taaaagcaaa gaaaatccca taaatcatgt catgggaccg 44581 tcaggagttt gtactggtgt gtaaagaaaa ttctttaaat cttgtcagga aaatgtcagg 44641 agtttagact ggtgtcttac acagtggcaa ccaacatagt ggcttttaat tagccatcct 44701 gcatccacca cttaggatgt ttatttttgc tcttgaaaga ttttcagaaa caagcaaagg 447Sl ggaagagcca aatcatttac acacatgcat aaccaaaccc aaatcggatt gttcacagaa 44821 attttaaccc aggcatgcag atcaaacaaa atattaaact aggcatgcag aaagaaccaa 44881 aaatgaactc accagaaaag acatgtctcc aagacagatt gtaaatgttg tagaaaccgg 44941 agtactctcc acaaggacac ttctcaggaa gagcttgcca aaaacagaat tttatagttc 45001 caagagagat gcagcattct tcatttaagg taggctgata accaaaccaa atctcagata 45061 aagtMaaaaa gcctctatca ggaagaaggg gcttagcctg agtgaagact caccagggaa 45121 gaacaggtga gccatggagg cagagagctc aaagggctca aggtgagcac agcctgcagg 45181 ttccaagaat cactgattcc ttccaaaagt gatcttgctt caggttccgc ttctggagac 45241 catttatgtc aacctaaata acaaatactg agaggttctc taaaagaaaa tgatgtttac 45301 atggtaatgg gtcattgcaa tgaaaatact catgccatat aacttatgtg catattctgg 45361 gaagtaaaag aagacaaagt tttttaaagg aaaagtaagg aggattacat gattgtttgg 45421 agagagttat ccttagctac aaggaacaat atgggtggtg ccagttccag gttggacagg 45481 cagttgctgg gcagatgtcc ttatagaagt atttttttgt gcaaggttat ggtttttgcc 45541 atctttcatg attgttcttg ttatcagaca tttgtgagtg agaattccca cttcatggcc 45601 ttccccagct ctattcatca ggatattttt taataacatt aagtgactcc atatttattc 45661 tgacaacttt cacaacatac aggtcctttt gtttatcagt caaggaaaga agaaacaaat 45721 aaaagtcaag tatattgagt gtttaatatt tactggactc taagagtaca aagatgaact 45781 ttttgctctt ctcaggaagg gagaatcaRa cacatcatac tgttgccata ccaagggatc 45841 atcgggagga agcYagaagg gagggggtgg ccatgggagc acagggtagc ctcagggggc 45901 aggaaaggca cctcagaggg Kctttaatgt ccactgcctt ggcactgtcc tggggaggga 45961 caaaattctc ttggtctctt gtgccttagt tctccagcaa gggtgactaa tggaStttaa 46021 ttccaggaaa catagctgtg ttatggaaga gcagccacac acagctgggc ttctgggtct 46081 gctgtcacac tcagctggtg ccacaggtag gcagaaaagc tatttctcag agcttcttag 46141 ccttggaatt gaggaatggt gtggacccca gcaatcttaa ttatgctttc tccaaatgct 46201 ttgtgaaccc ccaaaatcYg agagaggtct cagttaattt agaaagttta ttttgccagg 46261 gttgaggaca cacacccatg actcagcctc aggaggtcct gaagacatgt gcccaaggtg 46321 gttacagcac agtttggttt tatccatttt agggagacat gagacatcga tcaacatatg 46381 taagatgaac attggttcag tctggaaagg cRggactact cgaagcaggg aggggacttc 46441 caggtcgtaa gtagataaga gacaatggtt gcattctttt gagtttctga ttgacctctc 46501 caaaggaggc aatcagatat gcatttatct cagtgagcag aggggtgact ttgaatagaa 46561 taggaggcag gtttgcccta agcagctccc agcttgactt ttccctttag cttagtgatt 46621 tgggggttca agatattttc ctttcacagc tttaagccac tctttttcct aggtgacatt 46681 ttagtttatc aggtcacagg ttgctggcac actcatccct gttctcaaat atcttttaaa 46741 cctgagagaa atgacagttg agggaaactc atttacagaa agacaaagaa gacYatgtaa 46801 atttgcacta aagcataaat cttcagtctc tctaaaatga ttttctctga attctgaata 46861 cctgacatag tagaatattg gattcttgca taaacccttg acgttcacaa catgtttgtt 46921 tagtgggcag tgaaaattgt taacctggct tattattaaa aggaaaaata caggtttctg 46981 aaaggatttc ttRcttgatg tcacccaggt aaccatgcta ggagacagat ctgatattaa 47041 aattccttca gctagctgga ttacaaacct tctagggttg ctgacctctg aaatccaggt 47101 gattttgata atttggagtt tagtgatata caatcaggtt ttccagacct gtgttttttt 47161 tgtttttgtt tttttgtttt tcacctctgc ccaaattctt acagaatttg gtcataagtg 47221 ctttctcctg agtgatagga tgggaaggaa gagatttaag aactggaaac actgagagga 47281 ttacacatct gttgagcaca aactccaggg cttgattttt gtcaggttct gttctggact 47341 tgaaaacaca ttcagtcatg atttgtgcct ttaaggagtc actacctgtg catgggcaat 47401 Ycttctttaa gtctggaaag gatggtgatt aaagtagatg gagtatcagg ttctcagctt 47461 acctgggctc tttctttcac gcacttgtgg gactcattga gagttatttt attcactcat 47521 tgactcattt attacccttg aactgcttat ttgttccacg ttaaaggaaa caaggaatta 47581 ggaatctggg ttttattccc actcatacca caaactgatt gtgtgactaa gtgggcaatt 47641 tgactgctcc ttacctgagt tcatacgttt gtaaaatgca catcataatt cttgccctaa 47701 Stgactgtat tggttacttt tgagaatcaa gtgagaaaaa tggatattat agcattttgg 47761 caaatattta atgtacccct atgagacagt attattgttt ttaggccggt ggttctaaac 47821 ttcagtatga gtaatgatcc ttagagaata tatgaaaatg cttattactg agcctacatt 47881 cacagattct gagggtctgt gacccaggaa tctgcatttt aagaggcact tcaagtaatt 47941 atgatgcaaa tggtccaaga attccactga aaaatactgg tgtgtctgca gaatattggg 48001 gaattcctca gcaaggtgtc tgttttcRgg atgatctggg ggccctcatc ttgaagcctt 48061 tgccttgggt ttatctgtac atactcagaa ggRatttctc ttatttctca tgtttgtctg 48121 catgccatgc aaggggctta cactcaatag gtgcttagtg gaaatgtgct ggcctgccct 48181 acgtgcagaa aaccatgatt ctgcaggtga atgggctgga gtgtaagcag tcttttattt 48241 tcttttttta aatagcaatg cagagtcctc agagaagaga ttcagaatga acagctttgt 48301 gtcagacttt ggaagacctc tggagccaga taaagtattt tctcgccaag gcaacgagga 48361 gtccaggtct ctctttcact gctacatcaa tgaggtggaa cgcttggaca gagccaaagc 48421 ttgtcaccag accacagccc ttgacagtga cgtccaactc caggaagcca tcagaagcag 48481 tgggcagcca gaggaggagc tgaacaggct catgaagttt gacatcccca actttgtgaa 48541 cacagaccag aactactttg gggaggatga tcttctgatt tctgaaccac ctattgttct 48601 ggaaactaag ccactttccc agacctcaca Yaaagacctg gattgagaaa catgctctgt 48661 aaagggtctt cctgaagatg tggattctat ctttatgtag caagaaatct acatccacca 48721 aaattgtgtg tgtttggggg agagaRagac atagagatag agacagagag gcagagaaga
48781 gaccccttta gaagagagct gagctgatta agctgagtgg ttttttgttt tgttttgttt
48841 ttgcttttta atacatttgg agctttggga gtattaaagt atttacacca agcttgtcca
48901 acccgtggca tgtgtcccag gacagctttg aatgtggccc aatacaaatt ttaaacttta
48961 ttaaaacatg agttttgttt ttttttttgc tatttttttt aaagctcRtc agctatcgtt
49021 agtgttagtg tactttatgt gtggcccaag acaactcttc ttccagtgtg gcacagggaa
49081 gctaaaagat tggacacctc tgatttatac tagctcgtYt tgcttgttga aaaatttggc
49141 caaataccta ttgtcagcat tcttgggtga ggattagcct accatgttct aatctggccc
49201 tgccactact atgctctacc tttggtgagt tgctttacct ctctgggctg ccccattttt
49261 aactgtaggt tgacaggtct agagtgatcc atcccacctc taatattttg tgaatttatg
49321 actttgcctt cagatgaggc tgagctatac ataaaacagt ataaactagg gtactgcctc
49381 gtatctcttg taggctctct caaatctctg taccttccac ttaaccctaa ttgagccaag
49441 ctttagtcag gggatctggt tgtctaccag aatgtcagga gactcatctt acacagtcat
49501 ggtggccaat gtttctggtg ggttgtgctg aaacagctct tctgagaact tccaaccacc
49561 catgctctaa cctggagaca gccatcccct gcctcagaat aagtaccaat tYgtagtaca
49621 tgtatggtac tcttgtcccc aagaaatgtt aggaagcttg tcagctgaat gagaggaggt
49681 gccttctggg tatctctgtg ttggtgtatc tgtgccattg gctacagaac aagaaaaata
49741 ctatttgcca tgctattacc ttggcagatg tgtaggtgat agtcatctgg ctttgagctg
49801 agatggtcag tgggttgtaa attYcccact agcagatatt cagggtggcc tgagttatgt
49861 aaacaagtga gcaacacagc tttaatttca tggaggaatc aaagctgcac actggtatta
49921 aaacaacttg attttgcRYa cacagttgca tgcatggcaa gctgttaacc tctgggtggc
49981 attttcatta tgaatttgtt caccacctgt cttgcttaag ctacaaaata aatgcatttg
50041 actgcacaga aatttcctct ggttctttat tttcacacat ccaatttttt gtttcccaaa
50101 aagttgtacc atgtttataa tgtctcctRa cttcttggag taggcagtgc tgactgtacc
50161 atctctggga ttctggtgat ctgagccatg catgagattt agggattatt tcgctaaaag
50221 aaatcccttt tctattgtgt attttaaaga aaatatcatt ctttaagact ctaaatagta
50281 aaacaaagca tggaattttt ttgtaatYaa agctacttta acatattttt tataaataga
50341 tgctattttc atgaattttt tatgcaagag agcaagagta ttaaagaatg ctgagtttgt
50401 taactttttt tttttttttt tttttttgag agacggagtc ttgctctgtc gcccaggctg
50461 gatggctcac tgcagcctcc cctcctgggt tcaagtgatt ctcctgcctc agcttcccaa
50521 gttgctggga ctacaggcac gtgccaccac gcctagctaa tttttgtatt ttttagtaga
50581 gacgagattt cactatatgt tggccaggct ggtctcgaac tcctgacctc aagttatctg
50641 cctgccttgg cctcccaaag tgctgggatt gcaggtatga gccaccatgc ctggccagag
50701 tttaacttct aatatgagta tgattattca taatttattt aatgaaatat aaatcatggg
50761 ctattattaa agttcacagc tacttaagtg gtatcttatt tagaagtaga tttattgatc
50821 agaagattga aagatactta aaYgtcagac aaatacccat gactatatca tcatttttat
50881 gaactaaatg ttaagaactg tgaatggtcc aagattttgc cccttctgca aatcagcttg
50941 ccatagtctc atggatgctg ccagaagaca caagagtctt gggtcagaga caaaaatctt
51001 cattacagca cagcaggcca catatgcttc atgctcatgt tggttcctga gtttgtttcc
51061 tactgctgtt gtaacgaatt accacaaatt tagtgactta gagcaacaca aatttattct
51121 cttccagttc tagagtagac aagtctaaaa tcagtttcag tgggctaaaa tcaaggtgtt
51181 agcacagcag cattccatct ggaggctctc aggattaatc agttttcttg ccttttctat
51241 cttccagggg ttgctcccac tctgtggctc ctcttcttcc actttacatc tctgtcttta
51301 accatcctgc ttctctctta taaggacctt tgtgattaca ttgggttacc taagatgatc
51361 tccccatctt gagatccttc atttaatcac atctgtaaag tgtgtgcctt ttgccacata
51421 aggctacata ttgacaggtt tcagggatta ggatgtggac atgcttgggg aaccattagc
51481 ctgctagtct tccctttggc cccaaaggtt tatatccatt ctatatgcac aatacataca
51541 ccccatccca atatactcca cagtctcaat tcattataga atcaattcaa agtcacatgt
51601 attacctaaa tcttatcagc ttaaaagtcc caaatcttat tatcttaacc atctaatttc
51661 agtattagtg agatcctggg tatgattcat cctggggaaa aactctccat ctgtggacct
51721 gtaaaactag aaaataagtt attttctccc aaaatacaat gatgtgacYg acataagata
51781 ctagttacta atagttgtct ttcagtatat gcaggggatt ggttccaggc cccctgtata
51841 caccaaaatc ctagcatact taagttccac agtaggtcct gcagaacccg tgtatatgaa
51901 aattagctct ccgtataggt gggtttcgca tcccaagaat actgtatttt caatccccat
51961 ttggttgaaa aaaaatctgc atacaagtgg acacgtgcag ttcaacccta tgttgttcaa
52021 ggatcaaccg tattcccatt ccaaaaagga gaaaatggaa ggaaaaaatg aatcatcagt
52081 ccccaaaata acaaatcctt caaactggga aaactgttaa gctgcaaggc ctggaaatag
52141 tcttttgtga cttctggcta cattctctgg gcctgaagct ccactcttgg aatcatcttt
52201 tctttttctt gaaaggtaca atgtgtttKt agctgagtag ttttatcagc ctttttttct
52261 gcatgtagaa ttttgggagt ctgatagcct tcatttatct tgccctcact ctgtccattt
52321 cagttRaagt tgttatcagt gccctgggtt cttgtaatct tccagtatag acatcaagag
52381 agatcaccaa acataggagc aaagagaaag agagagaata gggcaagaaa aatagagaga
52441 gagaaagaaa aagggaatgg ggacttatcc tatgtaggaa cttgactcac cgaggcacca
52501 gggtgaaaca atggtgcagc tgccttggtt tgtgcctggt cagccatctc tgtcgtctgg
52561 gctgtgccat ttgctcccac aggaccctaa aatgagggtt ctatctggag tgccatgaga
52621 tgtaatcagc accctgggtt cttgtaatct tccaggatgg aaatacagat caccagacat 52681 aacagcaaag agaaagagaa agctttattt tagcttgtac acaaggaagt cagcactgca
52741 caaggaaaag ggcaggctgc tccctgaggg tagtatgtga gtcagtttta cagggccttt
52801 ccatagataa gtgttttatc agggcatgta taggaggagt ttctctagcg cttgggcagt
52861 ggcttcacat gcttctttgt acatcacatg tcacaataca ttttaaatct ccacccctgg
52921 tagtgactta gtgactttta gcattaaaat gaggaagggg tagctatgag ttgaagttta
52981 agtctaagta tgcatatggg gcctcaggga agtccctaat cccttaaagc aggaacttgc
53041 ggttaacagc ttcttggatc ttcttttgct gattggctgg aagttaggta agctacaact
53101 tgagtaagga gcttttgttc tttttctcta aaccactcca aaacagaaaa cgaaccagcc
53161 tggctgtctc aaagttggca gtgtttctgc tggtataaca ttttcaagaa ctagggtgtc
53221 tcctggatat atcacagaaa cttactacat taggcaagag gctcttctgt ggatcactcc
53281 tgaataaccc tatctctatt cctagttagt acttaaatgg ataaaatgat ccatgagtca
53341 cactcctaat tttttcagag agccccccgt gtgatttaat actctttttg tttcttttga
53401 gacaatagca aaggttgcct agtcacagtc cttggctttc tctccagagc atgctttcct
53461 gatgatgaat ctcctaattt tagtgttttt gcaatataat tggctgagaa tttcccaaat
53521 catcaatcct gattcctctt cacttaaagt tcttccctcc atttttcttt cctcttgcat
53581 tttactatca gtagcaagaa gaaaccaggc ctcatcttta acattttgct tggaaatcct
53641 ctcagctaaa tattcaactt tattatttac agattttgct ttctacataa ttgcagggca
53701 caattcacct aagctttctg ctgctatgta acaaggatct ccttttctcc agtttccaat
53761 aacatgttcc tcatttccat tttaattttc atctgcagta cctttagtat ccatatttct
53821 accaatagtc tgttcatgat gatttagata gttcataaga taattttttt tttttgacat
53881 ggagtctcac tctgttgcca ggctggagtg cagtggcgtg atctcggctc actgcaaccc
53941 ccRcctcctg ggttcaaacg attctcctgc ctcagcctcc caagtagctg ggactacagg
54001 tgcgcaccac cgcacctggc cagataattt tttttctatc atgctcagca cttccctctg
54061 agtcctcact tagagttgtt cacatccata ttcctactaa caggctactc aaggcaatct
54121 agtcagttta tatcatgttc ttcaatattc taccagccac tgcccattgc caactccaca
54181 tttttagttg tgttttgttt ttttacaaca gcaatagaaa ttaatatagt tcctcttgcc
54241 tccaggtctc acagtgtgga gaagcaaggg ctcaaggaga tgctgtgcac acagtgggtt
54301 ggcatcatgg ctgaggaacc ctgagcttag gggaaccctg tgttttgaca atgggttgca
54361 agcaaacctc cctggccttt acctcagaag gagatattat ctttgttatg ctggatagta
54421 aataaacctg ctctRtgctc cagagggaga cactatctcc atcttccaag gctgttcact
54481 ttgcaaacat tgttgaatag acagctgaga caaaggctgt cagttttccc ctgctcactW
54541 tacatgcaga aacctgagag gcctttggaa actgcctccc aaacctaaaa tgatcaaaga
54601 gtatattgca taagtcactg gggatttttg aaaaagaggc aactttgaag aagttgtgaa
54661 aagaatgcag gtgacttttt aaatgcctga atttctcttt tagagatata caaccacctc
54721 aggttaaatt caatggattt tttcccttct gaaagctgcc aagactttgc caaaatatat
54781 actgtgtatg tgagctattt tattacctac tgaaaaattg tctattattg gtagaacaat
54841 aatccatcct gtcacccaga aaagatgtgt agtagaaata gaaagtcttt atgtctaggc
54901 caggctttta gatatattga gagaggcgac ttcaggcagc ctgtgatcag aggtgttgag
54961 ttggcagctt gtgctgattc tcgaggcaga tgtgtgtgag tggcacagag aaaatattct
55021 tgtctttcta tggagttgga gcttttgaat aaattttgca gaaaacttgg gatcctcagc
55081 taagaacaat ccttgggaag ttcatctgca actgtatacc tagtgagagg tctccagaga
55141 aataYgcctg ccttgggaag aatgtgtgta cattttaagg aaacatagtt cataaaatcc
55201 atactggaga gagaggtatt tacatttcta ggatcttttc cacctgttct ctatggagta
55261 aagttttttc ttcctcatgt tttgcctctt tatcctgtat ataaacagag aaactctgtt
55321 tttctccatc cctcgtctag gatacagatg ttgtatgtat aggagatttt aacctagttt
55381 gcaatgttac ttcaggggta gaattgggac gtgagggacc atgtggttat ggatggcttt
55441 taaaatgaat aatgagaatc tgaacctacc cagataacct tgggtataca tttatgataa
55501 aattaataaa gacaaatgtt aaaaatttaa cacaaatgtt gttcgacttc attccaaaaa
55561 cacctcatta gatagcagaa tagtgtcaag tgtaagtgta gtacaagttg gcccacacag
55621 tacccacaga ccctatgacc taccagtgtg agtttgaaag caccagcctt tgacttcaag
55681 tgaaatgtat gcgaatgact tgcattaaag aaataataaa ttcagagata attatgataa
55741 caacagtgtc ataggttgtg ttccataaaa gcctgaaaag atttattgta ggtgagctat
55801 aagaagggga atgatggaag cagttagagc aggggaaaag ccaccaagaa tgtcatctca
55861 gctgcagact ggccacattc tgatctcaca ggaagttctg gggcacaaat tgcactacag
55921 aatcagtccc atcctgaggc aggggcatga tcttttgttg ccccaagcca gtcagggtaa
55981 gatggcttct gctttgatca agggtaattc ttgagagaag aaggtgctgg ttaacatccc
56041 tagcagctgg gaacaggcat tggctagtaa aggaccctgt gtgggcacta gcagcatgca
56101 ttacaaacaa taacaggtga catttattag gcactaagct acccctgtcc taaattaatt
56161 tttgtaagcc tactatgaga actttcaaca gagaggtcta agtgatcctt taaatatcat
56221 aagtcgggtt acttctctgt tcaaaatcct tcaatggctt cccatttcat gcagtgaaaa
56281 agccacagtc cttatgatga cctgaaagct ctacatgatc tatgtcctac tctcctgccc
56341 atcacagaag cagcccttcc aataactttt atatgtgtgt gtgtggtggg gtagggcagg
56401 gtggtcttag taagccaaga gtcattacat ctgggatgag cccttttcct gaatagatac
56461 gagcccactc ttttcagggc actgtgttgt cagctcacaa gcttacgatt gtggctttaa
56521 gtttcctctt tatttttgtc acctggaaac ttcactttcc ttcatgctta gctctatatt
56581 acttttgttg ttgtatttta tccaacatta ctaRaagttt tatttaagag ggattcaaat 56641 tatctcagtt cactgtgttt ccagaactgg aaacatgtta tttattcagt attagaatac 56701 ctgtttgatc cttcttaaaa atgtatttag tatatttctc tggtgaaaat ctttgtcttt 56761 tcatctattt tgtccatcat ttcctctaag ctcttaaaat attgcaatta attagtttat 56821 agtttttata aaaagactct actaactcta atgcctaaat aatctgtgga tttgcatcta 56881 ttgtttaatg tttcttattc ttatattatt aatcatatgg tcttgacatt tcacatgtct 56941 agacattttt ■tattatttgg cagacattgt atatgaaaaa tccattgata caacatggga 57001 aattatcttc catgagagat gtattcctct ttcctctgtt aagtagatag ggctagaggc 57061 tgattatctc agtcg-aatca ggcattgaag tgggtcagga ctgaattgca tttttaaaat 57121 aactttgaga tataattgac aaaataattt ttacatattt gaagtgcaca atttaataca 57181 ttttgacaca gtatacagtc aagaaaccat caccacaatc aagataataa acatgtccat 57241 catccataaa aattatgatg cccttttata atccctccct cccatccctt tctctgaaac 57301 tacagatctg ctttgcatta caacagatca gtccatattt tcatggtatt tatataagtg 57361 aaatcatgca gtacatactc tgttttgcct ggcttctttc attcagcgta attattttga 57421 gattcgtcca tgttgttgtg tgtatcaata gttcattctt ttcattgctg agtagtgttt 57481 cattgtgtgg ctatacatac cataatttgc ttatctattt atttgttgat ggatatttgg 57541 gttacttata attttggcta ttgtaaataa atattttcta tgaatatttg ctgtaaatat 57601 tcatttataa gtcttcatgt gaacatgtgc ttctacttgt ttaagttcct agaagaggaa 57661 tgtctgggtc atatggtagg taggcatttg tctttttaag aaactgctaa acagttttcc 57721 aaaatggttg tatgatttta actttctcat agtgtatgtg aattctggtt gctccacttc 57781 ctttccaaca tattgtaaca tcagtctttt aaattgtatt cattctaata tgcatgtgta 57841 gtagtatgtc atttttcaat tgcatttctc taatggtatt cagcattttt tcatatgctt 57901 atttttcatc agtatatctt tagcaaagtg cctttcaagt ctttgtccca ttgttttatt 57961 agggaatttg ttttattgtt gttttgagag ttctttatat tctgtaaata taagttattt 58021 atcagatata ggatctgcaa atagtttctc tcagtttgtg ggttgtcttt tcatatcctt 58081 aacagtgtct tcaaaggaca gaaattcttc attttggtga actctaatcc ataatttttt 58141 taatagatat catgctttta gtgtcatatg aaagaaatct ttgccaaacc caaggtcata 58201 aaggtataat cctatgcttt ttttttctag aagttctata gtttcagatt ttcattaagg 58261 ttcgtgattc attttgagtt tttttcttaa acatggtgtg agatcaccat gatcttttta 58321 atgagagatc aaaaaagatc cttttctttt tttttttttt ttgcttatgg atatctaatc 58381 gttccagcat ggtttggtga aaagatcttt tctccagtgt attgcctctg caactttact 58441 gaaaatcagt tgtctatata tgtgttggct ttatttattt tttattttta aatttttttt 58501 acaattctgg acattctatt cacctgtttg acactcattc cacacttctt gtttattata 58561 gctttagaag tcttgaaatc agtagtaata tagtctggat atttgccccc tccaaatctc 58621 atgtttaaat gtaacctccg atgttggagt tgggcctagt ggaaggtgtt tgggtcatca 58681 gggcagattc ctcatgaatg gctgtcctca cagtaatgag tgagttctct aagagttcac 58741 atgagagctg gatatttaaa ggagcctggc acctcctcct ctccttcttg ctccctctct 58801 cagcatgtga tgcacctgct ccccctgtgc cttctccatg agtgaaagct ccctgaggcc 58861 tcaccagaag ctgagcagat gctggtgcca cacttgtgca gcctgaataa cttaactgtg 58921 agccaaacaa acctcctttc tttataaatt acctactctc agKtattcct ttgtactaat 58981 gcaaatggat taatacagat agtgtatatc ttcttcttct ctctctcttt tttttttttt 59041 tatttgagac agattcttgc tctgttcccc aggggagtat agtggtggga tcttggctca 59101 ttgcaaactc cacctcttgg gttcaagcga ttctcccgcc tcagccttct gagagctggg 59161 attacaggcg catgccccca tgcccagcaa atttttacat ttttagtagg gatggggttt 59221 tgccatgttg gccaggctga tctcgaactc ctggccccaa gtgatccact tgccttggcc 59281 tcccaaagtg ctgggaatac agacatgagc caccataccc agccaagtct tctaatgttg 59341 ttcttcctgt ttatttttgt tttgactatt tggcatcctt tgcatttcta tatgaatttt 59401 agaatcagtt ttttattttt atttttattt gtttatttgg tttttgagac agggtctcac 59461 tctgtcaccc aggctggaat gcagtggaac aatcgtggct cactgcagcc tcaacttccc 59521 tgggctcaag ccatcttccc acctcagcct cctgggttgc tgagactgca ggcacctgcc 59581 accacaccta cctaattttt ttcttgtatt ttgtagagac agcatttcgc catgttgtcc 59641 aggctggtct tgaactcctg ggctcaagca atccacctgc ttcagcctcc tgaagtgctg 59701 aaattacagg tgtgagccaa catgcctggc cagaatcagt ttttaaattc cctgtcttac 59761 accctgttct ccctaaattc cactcatttc ttaagataca acttgaaagc tatatttttc 59821 aaaaaatgtg ctctagatcc ctggagaaag aaatttctac tttctctgtg ctctcttcac 59881 acttcataac atttttattg catatattca catttggcta tatgtacatg tctgatcttt 59941 tctgccacac agtaaacctt tcaagatcag gaatcttggt gtgttccatc tgtctgcttg 60001 aaaaactagt tttatacttt gcaggagtga gccctgtatt aagagagacc tgcagaagac 60061 atgtagctca tttcctgcat attgtcaagg catccagtag atttacaatt gtttattcta 60121 ttgtttgatg tctccaagat ctctcagtct tttattattt ctattgacac taatgtactt 60181 tcaaagaata atgggattat tattttccaa atatccaaac atactctaat tgacttatga 60241 tggtaacatg cagctgcatc ttaatgtatt cattgttcac atcctccttc atccttgaac 60301 tcttggagga atgtcacatc attaaagata ttgatatctt tggctagcag tgcaatagta 60361 catcaactca gacctactga atatacactg gccctttatc ttattattct caggaagatt 60421 attttcatgg agtttaatta tgtccttctt agggaagaaa ttcagcttag tgatctggca 60481 gcatgggaat acgtgtagcc tgctttttgt agggcctaaa ttgtttcata cttattaggg 60541 acaaatacaa tacagaaatt gttttagcat ctgacaagtt agcagcataa ctatatgagt 60601 agggaaatca gctttcccat cccactcagg cattatgcaa gtgtgacact caccttgggt 60661 aatggtttcc cctgtgagca gtttaaggct tcaggacagt tatgaatttg gctttgtgtt 60721 tttcttcctt agtctttcca cattgattac acctggtgag attatatgaa tcctgtttat 60781 ttgcagcata gcactaatct ttactaaagc atcatatgtt taaaagctag tagataattt 60841 gttagaaaat agaaatgctc tcagatttca tcctaaagta gaaacttgat tagtggttaa 60901 agaaaattta tattaaaaaa ttaaatacag agatatcaaa acaaaaaatg attattagtt 60961 gattccatta aatactatag cagtcatttg cctttaagcc tatttctatg gaagctttgc 61021 aatgcttctc tcttaaattc catcaaagtc ctgtgtcatt atcatcatta tcttcatcat 61081 catggaatta attgctctgg aatatcccag ggaattaaag agttgaacac acaggggatg 61141 tgtgtaagtc agtgtgtgtg catgtataca atgagaataa taataagaca ttagatcttg 61201 gatatatatt ccattgagag aaggatgtga tcaggaaaac ttggtgtggt gaaaaaataa 61261 agcagtagga gtgagatggt caaatggaaa gaagtcatag caaaggtggt agcagagtgg 61321 catggttaaa aaattcatag agcggtttga aggaagggga gtgttgtaga caaaataatg 61381 ctacccacca ctcacccacc tatgtccgcc tgtgaatatg ttatttattg gcaaaagggc 61441 ctttgcagat taaggacctt gagatgagga gactggaaaa tgagcttgca ttatccaggt 61501 gggcccaatc taatcacatg agtggggcat aaaatcagag aagctgagtt agagcttgag 61561 ggagatgtga ccatggaaga aggctcagag agatgcaaag ttgctggcta tgaagatgga 61621 ggaagggagg cacaagccaa ggaatgtgag tggtttccag aagctggaaa aggcaagaaa 61681 acagattctt ttctagagca tccagaaaag agcacagtct tgccgatacc ttgattttag 61741 cccaacaaga actgtgctgg acatttgacc tacagaactg taagacgata agcttgtgtt 61801 gttttaagtc attatgtttg ctataacttg ttacaacagc aatagaaaac taatataggg 61861 aggatttatg taagacatta gaggtctgtt atggagtgtg gataggaagg ctgggatcag 61921 agtgaggagg acctcggtga ttaaggcaag gattctgatg tttacaactt aaaaaaaaaa 61981 ataatgtgag gtgggcaggt ggaatgtgtc aagcaatttt ttaggaagaa ctacctgtca 62041 atgctgttgt agactatttg gagtgatggg agcattggag tagaggaatc agttaagctc 62101 ctactagaaa atcaagaagt aaaatgatga cagagcttca gtaatggtgg tagctatgaa 62161 aatgtagaca gacaaatata tacattatct tgaagaaagt ggctacaagt ttgatgactc 62221 catgttggag aagataagta aaggatgctt caaagtttaa aactggctga atagagatga 62281 tgaaacatag tgagtaggca gctgagttta atgaggacag attctggcag aaattggaaa 62341 cagatgttgg cattcatgag caaggaaaga ggaagggtag aaacgtagat ccaatgttat 62401 cttgttatcc cactagtagt ggtatttgtg ccatgatggt agataaaatc tgtggggatt 62461 tgaggggagg gagtgcttta tttgtggaag gagatcaagg gatgacaaag gtgattaatt 62521 tcctgagccc ctactttgtg tgtgaggctg gactgctgtg aaggctctga ttccaaatca 62581 gggggtgagc acctgccNtt tttttgctac agcttcattc actcattcct gataatgagc 62641 aaaataggta agaagacatg agattgcaaa ggtatgattc cttaccctga cagcccagag 62701 ctggtgaaag accaagatgc tttattgcac ccagtttgct ccagtaagca ggatcaattt 62761 tgccctttat aaataattca aattctttcc ttcatgaatt cacttgtgtg tacctcacaa 62821 atccttttca gaaaaccatt ccagactttt ctccaagaag tttcatagac cttgtgtagc 62881 tctatttcta ttatctgtcc tctgtatctg cataagggtt ttgttattgt aataccaata 62941 ccattagata ggaaccattg gtaccatgac cataagtgca gggtacgctt tactgagact 63001 aaatatcctt ggtcaggctc ctgctgtgtt cttgacttca tttttgtgct ccaagaatgc 63061 ataacttgta taattacctt ctccatttgt ttggtctgct cctctatagt ctactccatt 63121 tttgttttac ttgaatttac aactcatttt attctactaa agaaacotga tgcctcttct 63181 cctatagatt tcccctcaaa ttaatactgt acttcaacaa acttaattct tccaacaatc 63241 ttataaagca ggtatacatg ggaaagaaaa gggtttccct caatttgtat tatttgggta 63301 gctgtgccaa ggcttggggc ttgaatcctc tgaagccaca ggccgagctc tacattggcc 63361 actttcagcc atggctggag tggctggaat gcagggcacc aagtccctag gctgcacaca 63421 gcacaagaac tctggttcct gcccacgaaa ccatcttttc ctcctaggcc tctgtgcctg 63481 ggatggaagg ggctgtcatg aagacctcca acgttccctg aagatatttt ccccattatc 63541 ttggggataa aaattcggct cctcattaat tatgcaaatc tctgcagctg gcttgaattt 63601 ctcctcagaa aatgggattt tcttttctat cgcatagtta ggctacaaat ttttacaact 63661 tttatgctgt gtttcccttt taaaattgaa tgcctgtaac agtacccaag tcacctcttg 63721 aatgctttgt tgcttagaaa tttcttccat cggctgggcg cagtggctca cgcctgtaat 63781 cccagcactt tgggaggcca aggtgggtgg atcacgaggt caggagatcg agaccatcct 63841 ggacaacatg ctgaaaccct gtctctacta aaaatacaaa aaattagcca ggcatggtgg 63901 tgggcgtctg tagtcccagc tacttgggag gctgaggcag gagaatggca tgaacccagg 63961 aggcggagct tgcagtgagc caagatcaca ccactgccct ccagcctggg ctacagagca 64021 agactccgtc tcaaaaaaaa aagaaatttc tttcatcaga taccctaaat catctgtctc 64081 aagttcaaag ttccaccaat ctctagggca gaggcaaaat gccacgagtc tctttgctaa 64141 aacataagag tcacctttgc tccaattccc aacaagttca tctccatctg agaccacctc 64201 agcctggatt tcattgtcca tatcattatc agaattttga tcaaagccat tcaacaagtc 64261 tctagggagt cccaagcttt cccacatttt cctgtcttct tctgagtcct ccaaactgtt 64321 ccaacctctg cttgttaccc agttctaaag tagcttccac atttttgggt atattttcag 64381 caatgcccca ctctataggt actaatttat tgtattctgt tttcatgctg ctgataaaga 64441 catgcctgag actgggcaat ttacaaaaga aagaggttta attggactta cagttccacg 64501 tggctgggga agcctcacaa tcatggcaga aggcaaggag gagcaagtca tgtcttacat 64561 ggatgacagc aggcaaagag agagagagct tgtgcagcgg aattcctctt ttcaaaacca
64621 tcagatctca tgagacttat tcactatcat gagaacagca tgggaaagac ttgcccccat
64681 gattcactta cctcccactg ggtccctccc gaaacatgtg ggaattcaag atgaggtttg
64741 ggtggggtca cagccaaacc atatcagcat ggtagtacta agagacaccc taagggatct
64801 tctgtattcc agagttactg ttctctacct ccattgtgga gtagcagtcc aatttcccct
64861 tggcagYctg gattagtcac tctaggcaat atcataactt ccttcttggt ctgttgactc
64921 agaggcatga ggagcccaaa gtagctggat tgcaatcttc actttcaggt caatgaaatc
64981 attgttttat ctcttggtgg aagtatgctt ccctctggaa ctaaggcctc taagtcaaca
65041 gagcataaag tcacaggaac agaaagtaaa aattttgcta gtgggtcact aggggtaatg
65101 tggagtggtg ccattcccat ttccacccct tgattcctag acctatatat tttgccagta
65161 agaaaatcta tcacatatta gacactgatt cagagcatct ataaccttct tgagaacctt
65221 gccccagccc tgcaaggtac tgtcacttag ctgtcactgt aactgtatct tcaaatgaca
65281 atcccaccat tctatcaWgc cagctgcttc aggatgttga agaacatggt aagaccagtg
65341 aattccatga acattaggcc actgacatac ttctttggct tgtgaaatga ttttcttggt
65401 cagtagcagt gctttgtgga ataccatgac tgtggataag gcattctata cgtcagtggt
65461 tggtggtttt ggcagaagca ttacatgcag gaaaggcaaa tccatatcca cactgtctat
65521 tccactaagg acaaaatgct gccccttcca tgatggaagt gatccaatgt aattaacctg
65581 ccaccaggta gctgactgat aacccctgag gatggtgcca tattgaggac tcagggttgg
65641 tctctgctgc tggcatattg ggcacttagc agtggtcata cccaggaagg cttttgtgag
65701 tggaggtcca tgttgttgag cctatgcata tcctccatcc ctgactgcat attacagaat
65761 tatctgtaaa ccatgcctga gtttctcttc ctttgtcaac tgattgtagg gtactcccct
65821 gtgaggccat ggatgcaggc tgggagaaag aagacaatat aacaggagta ggaacgatgg
65881 gcatttgggc cacctcttca tgtaacatac ttatgccttc Rggacctgct caaggctgat
65941 cacttatata ccatttccat ttgatgatgg agtgctgctg tgcatgccca atttttacag
66001 ttcagtgggt cagacaacat gcagttcatg atagacagct tagtttgcat gggaactttg
66061 tggcttatgg tcaagggttc agtttctact aaggcccagt agcaggtaaa gagctgcttc
66121 tcaaaaggag agccatctat ggatgatggt agggccttgc tgtaaaatcg taaaggcttg
66181 agcttcaatt cacatatagg gtccttccaa aggctccaaa ccgcatccct gtcttccact
66241 gagacctcaa gcaccattgg atctgctgga tcatatagcc caaatggcag agcagtctgt
66301 acaacagcct agacctgttg gagaggcttc tcctattctg caccctactc aaaactagta
66361 gctttttggg tcatgtggta catgagacga gtaacacacc caaatgagta atatgttgcc
66421 tccaaaatcc aactaggtcc actaggcatt gtgccYcctt cttggttgta ggagcggcca
66481 gaagcaagaa tatatatttt accttagaag taatatctta ctattcccca aaacactgga
66541 cccctagaaa tttcagtgag gtagaaggac ccagaatttt agttgatttt atttcccacc
66601 ctctcacatg caaatgtctt aataagtcca gagtagttgc tacttcttct tcattggatc
66661 caatcagcat aacattatta atgaaatgga acaatgtgat accttgtgaa agggaaaggc
66721 aatcaagatc tttgtgaacc aaattgtgat acagtgctgg agggttgata taatcctgat
66781 ataggacagt gaaggtgtac tgctggcctt Rccagctgaa agaaaactgt gatacagtgc
66841 tggagggttg atataatcct gatgtaggac agtgaaggtg tattgctggc cttaccagct
66901 gaaagcaaac tgtttatggt tggttttatg gacaggtgtg aagaaaaagg catttgccag
66961 attaatagct gcatacagct cccaagagat gtgttaattt gctcaagcaa taaaaccaca
67021 tgtggtacag tagctgaatt agagtcacta cttggttaaa gtatagcaat ctgctgtcat
67081 tcctcaagaa tacaccaaac caggatatta accactgatc tagcaacaat gtgggatcag
67141 acatagcttt gatttgttgg tacatgtctt gcagggctat caagatattt cccaaattat
67201 ctgggatgta tgcacagcat ttagtcttaa ttattatgcc ctttccccac tgtcagttgt
67261 acttggaggt cctgtgggga tatgatgaca ggttggctaa atatatattt aatatatgtg
67321 tgcatgtgtc tttataacag agccatttat attcctttgg gtatataacc agtaatggga
67381 ctgctgggtc aaatggtatt tctgcctcta ggtctttgag aaattgccac attggttgaa
67441 ctcatttaaa ctcccatcaa cagtataaaa gtattccttt ttctccacaa ccttgccagc
67501 atctgttgtt gtttcacttt ataatagtag ccattctaac tggtgtgaga tggtatctca
67561 ttgtggtttt gatatgcatt tctctaatga tcagtgatgt tgagtttttt tttttatatg
67621 tgtgctggcc acatttatgt cttctttcaa gaagtgtctg ttcatgtcct ttgcctactt
67681 tttagtgggg ttgtttattt ttttctaaat ttgtttaagt tccttataga tgctggatat
67741 tagacctttg tcagatgcac caattgccaa aaatcttctc ccattccata ggttgtctgt
67801 ttactctgtg gataatttct ttttctagga agaagctctt tagttagatc ccatttgtca
67861 aattttgttt ctgtttcaag tgctattggc atctttgtca tcaaatcttt gaccatgttt
67921 atgtcctaaa tggtatcgcc taggttttct tacagggttt ttatagtttg aggatttaca
67981 tttaagtctt taattcatct tgagttcatt ttatatatgg- tgtaaggaag gggttcagtt
68041 tcaattttgt gcatatggct agccagttct cccagcacaa tttattcaat aaggaatcct
68101 ttccccctgg cttgtttttg tcgtttgttg aagatcagat ggttgtagat gtgtggtcct
68161 atttctgggc tctttattct gtaccattgg tctatgtgtc tgttcttgta ccaataccat
68221 gctcttttgg ttgctgtatc cttatagtgt agtttgaagg cgggtagtgt gatgcctcca
68281 gctttgttct ttttgcttag gattgtcttg gctttttggg ctcttttttg attccatagg
68341 caatttaaaa tagtgtttcc taattctgtg aagaatgtca aaggtagttt aatgggaata
68401 gcactgaatc tatacattgc tttgggcagt atgactatat gcatgctatt gattcctcct
68461 atccgtgagc attgaatgtg tttctatttg tttgtgtctt tctctgattt ctttgagcag 68521 tggtttatag ttttccttga agaggtcctt cattcccttg ttagctattc ctaggtattt
68581 tattcctttt gtggcagttg tgaatgaaag ttcattcaag gtttagctct ctcggtttgc
68641 ctgttgttgg tatataggaa ggatagcaat ttttgcacat tgattttgta taccgaagtt
68701 gcttattagt ttaagaagct tttgagctga gatgagggag gtttcttttc ttaaaacact
68761 caacttcttt attattatta ttgtacttta agttctgggg tacatgtgca gaacatgcag
68821 ttttgttaca taggtataca cgtgccataa ttgtttgctg tacctatcaa cccgtcatct
68881 acattagcta tttctcttaa tgctatccct cccctagccc cccacccccc gacaggtcct
68941 ggtgtgtgat gttcccctcc ctgtgtccat gtgttctcgt tcttcaactg ccacftatga
69001 gtgagaacgt gcggtgtttg gttttctctt cttgccttag tttgctgaga atgatggttt
69061 ccagcttcat ccatgtccct gcaaaggaca tgaactcatc gttttttatg gctgcatagt
69121 attccatggt gtatatgtgc catatttgct ttatccagtc taacattgat gggcatttgg
69181 gttggttcca agtctttgct attgtgaaca gtgccgcaat aaacatacat gtgcatgtgt
69241 cttcatagta gcatgattta taatcctttg ggtatatacc cagtaatggg attgctgggt
69301 ctggttctag atccttgagg aattgccata ctgtcttcca aaatggttga actaatttac
69361 actcccacca acaatgtaaa agtgttccta tttctccaca tcttctccag catctgttgc
69421 ttcctgactt ttcaatgatc accattgtaa ctgccatgag atggtatctc attgtggttt
69481 tgatttgcat ttctctaatg accagtgatg attaactttc tttcatatat ttgttggcta
69541 cataaatgtt ttcttttgag aactgtctgt tcatatcctt cacccacttt ttgatggggt
69601 ttttttttct tgtaaattta agttctttgt agattctgga tattagccct ttttccagat
69661 ggatagattg caaagatttt ctacgataga ttgcaaagat tttctaccat tctgtaggtt
69721 gcctgttcac tctgatgata gtttcttttg ctgtgcagaa gttctttaat tagatcccat
69781 ttgtcagttt tggcttttgt tgccattgct tttggtgttt tagtcatgaa ggctttgccc
69841 atgcctatgt cctgaatggt attgcctagg ttttctccta gtgcttttat ggttttaggt
69901 cttacattta agtctgtgat ccatcttgag ttaatttttg tataaggtgt aaggaagggg
69961 ttcagtttca gttttctgca tatggctagc ctgttttccc aacaccattt attaaacagg
70021 gaatcctttc cccattgctt gtttttgtca ggtttatcaa agatcagatg gttgtagatg
70081 tgtggtgtta tttctgaggc ctctgttttg ttccattggt ctatatatct gttttggtac
70141 cagtagtatg ctgttttggt tactgtagcc ttgtagtata ggttgaagcc agatagcatg
70201 atgccttcag ttttgttctt tttgctgagg attgtcttgg ctaaatgagc tgtttttttg
70261 gttccctatg aaatttaaag tatttttttt tccaattctg tgaagaaagt cagtggtagc
70321 ttgatgggaa tagcattcaa tctataaatt actttgggca gtatgatcat tttaatgaca
70381 ttgatttctt cccatccatg agcatggaat gtttttccat ttgtttgtgt cctctcttat
70441 tttcttgagt ggtggtttgt agttctcctt gaagcagtcc ttcagatccc ttgtaagttt
70501 tattcctagt tattttattc tctttgtagc aattgtgaat gggagttcac tcatgatttg
70561 gctctctctt tgtctattat ttgtgtatag gaatgcttgt gattctgcac attgattttg
70621 tatactgaga ctttgctgaa gttgcttatc agcttaagga gatttgagct gagacaatgg
70681 ggttttctaa atatacaatc atgtcatctg caaaaagaga caatttgact tcctcttttt
70741 ctatttgaat accgtttatt tctttctctt gcctaattgc cctggcccaa acttccaata
70801 ctgtgttgaa taggagtggt gagagagggc atccctgtct tgtgccagtt ttcaaaggga
70861 atgcttccag tttttgctca ttcagtatga tattggctgt gggtttgtca tagatagctc
70921 ttattatttt gagatacgtt ccatcaatac ctactttatt gagagttttt agcatgaagg
70981 gctgttgaat tttgtcgaag gccttttctg catctattga gataatcatg tggtttttgt
71041 catgggttct gtttatgtga tggattacat ttattgattt gcatatgttg aatcagcctt
71101 gcatcccagg gatgaagcca acttgatcat tgtggataag ctttctgatg tgctgctgga
71161 tttggtttgt gaatattttt ttgaggattt tcgcatcaat gttatcatgg atatcggcct
71221 gaaattttct ttttttgctg tgtttctgcc aggttttggt atcaggatga tgctggcctc
71281 ctcaaatgag ttagggagga gtcccccttt ttctattgtt tggaatagtg tcataaggat
71341 tggtaccagc tcctgtttgt aactctggta gaattaggct gtgaatccgt ctggtcctgg
71401 gcttcttttg gttggtaggc tattaattac tccctcaatt tcagaacttg ttattggtct
71461 attcagggat ttgacttctt cctggtgtag acttgggaga ctgtatgtgt acaggaaatt
71521 gtccatttct tcttgatttt ctagttaatt tgcacagggg tgtttatagt attctttgat
71581 ggtagtttgt atttctgtgg gatcagtggt gatatcccct ttatcatttt ttattgcatc
71641 tatttgattc ttctctctct tcttctttat tagtctggct agcagtctat ctattttgtt
71701 aatctttaca aaaaactggc tcctggattc attgattttt atgagggatt ttttatgtct
71761 ctgtcttctt cagttctgat ctgatcttag ttatttcttg tcttctgcta gcttttgaat
71821 acgtttgctc ttgcctctct agttcttttg tgatgttagg gtgttgattt tagatctttc
71881 ctgctttctc ttgtgggcat ttagtgctat aaatttccct ctacacactg ctttaaatgt
71941 gtcccagaga ttctggtaca tcatgtcttt gttctcactg gtttcaaata acatctttat
72001 ttctgcctta attttgttat ttacccagta gtcattcagg agcagttgtt cagtttccat
72061 gtagttgtgc agttttgagt gagtttttaa tcctgagttc taatttgatt gcactgtggt
72121 ctgagagact gtttatgatt tctgtttttt gcatttgctg aggagtgttt tacttccaat
72181 tatgtggtca attttagaat aagtgtgatg tggtgctgag aagaatgtat attctgttga
72241 tttggggtgg agagttctgt agatgtctat taggtcctct tggtccagag ctgagttcga
72301 gtcctgaata tccttgttaa ttttctgtct cattgatctg tctaatattg acagtagggt
72361 aaagtctccc attattgtgt gggagtctaa gtctctttgt aggtctctca gaacttgctt
72421 tatgaatctt tgtgctcttt tattgggtgc atatatattt aggatagtta gctcttcttg 72481 ttgcattgat ccctttacca ttatgtaata cccttctttg tctcatttga tctttgttta
72541 aagtctgttt tatcagagac taggattgca acccctgctt attttgcttt ccatttgcct
72601 tgtaaatctt cctccatccc cttatttgga gcctatgtgt gtctctgcac gtgacatggg
72661 tctcctgaat acagcacact gatgggtctt gactctttat ccaatttgcc agtctgtgtc
72721 ttttaattgg ggcatttagg acatttacat ttaaggttaa tatttttatg tgtgaatttg
72781 atcctgtcat tatgatccta gctggttatt ttacccatta gttgatgcag tttcttcata
72841 gtgtcaatgg tctttacaat ttgttatgtt tttgcagtgg ctggtactgg ttgttccttt
72901 ccaacttcag tgcttccttc aggagctcta gtaaggcagg cctggaggtg acaaaatctc
72961 tcagcatttg cttgtctgta aaggatttca tttctctttc acttttgaag cttaatttgg
73021 ctagaaatga aattctggct tgaaaattat ttaagaacat tgaatattgg cccccagcct
73081 cttctggctt gtagggtttc tgccgagaga tctgttagtc tgatgggttt ccctttgtgg
73141 gtaagctgac ctttctctct tgctgccttt aacatttttt ttgttaattt caaccttgtt
73201 gaatctgaca attatgtgtc ttggggttgc tcttcttgag tagtatcttt gtggtgttct
73261 ctgtatttcc tgaatttgaa tgttgccctg tcttgctagg ttggggaaat tctcctggat
73321 aatatcctga agagtgtttt ccaacttggt tccattctcc ccctcacttc aggtacacca
73381 atcaaacata cgtttggtct tttcacatag tcccatattt cttggagact ttgttcattc
73441 cttttcattc ttttttctct aatcttgttt tcaaacttta tttcattgag ttaatcttca
73501 atctctgatg tcctttcttc cacttgatcg atttgactat tgatatttgt gtatgcttca
73561 caaagttctc atgttgtgtt tttcagctcc atcaggtcat tgatgttctc taaactggtc
73621 attctagtta ggaactcctc taaccttttt tcaaggttct tagcttcctt gcattgggtt
73681 agaacatgct cctttagctc agaggagttt gttattaccc accttctgaa gcctacttct
73741 gtcagttcgt caaactcatt ctctgtccag ttttgttccc ttgctggtga ggagttgtga
73801 tcctttggag aagaggcatt ctggttttta gaattttcag cctttttgtg ctgatttctc
73861 cccatctttg tggatttatc tacctttgct ctttgatgtt ggtgaccttc ggatggggtc
73921 tctgcatgga cgtcctttct gttgatgttg atcccactcc tttctgttta tttgttttcc
73981 ttctaacagt caggcccact gctgcaggtc gctggagttt gttggacatc cactgcagac
74041 cctgttttcc tgggtatcac cagcagaagc tgcagaacag caaagattgc tgcctgttcc
74101 tttctctgga agcttcgtcc cagaggggca cccgtcagat gccagccaga gctctcctgt
74161 atgaggtgtc tgttgacccc tgctgggagg tgtctcccag tcaggataaa cgggggtcag
74221 gggcccctta aggaggcagt ctgtctctta gcagagcttg aacactgtgc tgggagatcc
74281 acagctctct tcagagctgt cagggaggga catttaagtc tgctgaagct gcactcataa
74341 ctgccccttc ccccaggcac tctgttccag gcagatgggt gttttatcta taagcccctg
74401 actggggcta ctgccttttt ttcagagatg ccctgcccag acaggaggaa tctagagagg
74461 cagtctggcc acagtggcct tgctgagctg cagtcggctt cacctagttc gaacttctca
74521 gcagctttgt ttacactgtg agagtgaaac ctcctactca ggtctcagca atggtggacg
74581 ccccttcacc caccaagttt gagcgtccca ggttgacctc agactgctgt gctggcagtg
74641 agaatttcaa gccagtggaa cttagcctgc tgggctccat gggggtggga cctgccaagc
74701 cagaccactt ggctctctgg cttcagcccc ctttctaggg gagtgaacgg atctgtctcg
74761 ctggtgttcc gggcaccact ggggtatgga aaaaaaaaca ctcctgcagc tagctcagtg
74821 tctgcccaaa tggctgccca gttttgtgct ggaaacccag ggccctgatg atgtaggcac
74881 cgcagggaat ctcctggtct gcaggtcgtg aagaccatgg gaaaagtaca acatctgggc
74941 tggagttcac tgttcctcat gtcacagtcc ttcatggctt cccttgggta ggggagagaa
75001 ttccctgacc ctttgtgctt cttgggtgag gtgaggcccc accctgcttc agcttgccct
75061 ccatgagctg cacccactgt ccaaccagtc ccagtgagat gaaccaagta cctcagttgg
75121 aaatgcagag atcacccgcc ttctgcatcg atcttgctgg gagctgcaga ccagagctgt
75181 ttctatttgg ccatcttgat ggggttttct agacttaaga tcatgtcatc tgcaaacaaa
75241 aaagagtttg acttcctctc ttcctattta aatacacttt atttctttct cttgctgact
75301 gccctggcca gaacttccag tactatgttg aataggagtg gtgagagagg gcatccttgt
75361 tgatatggtt tgggtgtgtc cccacccaaa tctcatcttg aattgtagct cctacaattc
75421 ccatttgttg tgtgagggac ctggtgggag gtaattggat cataagggtg atactttcca
75481 gtgctgttat cgtgatagta agtctcatga gatctgatgg ttttataagg ggaaatccct
75541 ttcactggac tctcattgtg tcttgcctgc tgccatgtaa gacatgcctt tcaccttctg
75601 ccatgattgt aaggcctccc cagccacata gaattgtgaa tccattaaac ttctttttct
75661 tcttaaatta cccagtctca ggtatgtctt catcagcagt gtgagaacag actaatacac
75721 ttgtcttatg ccgattttca aggggaatgt ttccagcttt tactcattca gtatgatatt
75781 gactttgggt ttgtcattta tggctcttat tattttgagg tatattcctt caatacctag
75841 ttttttgggt ttttgacatg aagcaatgtt aaattttatg gaaggaattt tctgcatcta
75901 ttgaaataat catgtgattt ttgtgttcag ttttcttatg tgatgaatca catttattga
75961 cttgtgtatg ttgaaccagc cttgcatcct ggggatgtag cctacttgat cgtggtggat
76021 aagctttttg atgtactgct gaatttggtt tgccagtatt tttttgagaa tttttgcatc
76081 gatgttcatc aaggataYtg gcttgccgtt tccttccctt cccttccctt cttccctttt
76141 cttcccttct tcccttccct tttttctttc tcttttttct tcttctttct ttctattttt
76201 ttcatgagat agagtcttgc tctgttgccc agtctggagt gtagtggtgt gatctcagct
76261 cactacaacc tctgataccg aggttcaagt gattccccta tctcagcttc ccaagtagct
76321 ggtattacag gagagcacca ccacacctag ctaatttttg tatttttagt agagacaaag
76381 tttcaccata ttggccaggc tggtcttgaa ctcctgagca catgtgatcc gtccaccttg 76441 gcctcccaaa gtactgggat tacaggtgtg aRccaccatg cccagcctga agtttccttt 76501 ttttatttta tctctgctga gttttggtat caagatgatg ccagcttcat agaatgagtt 76561 agggaggagt atctcctttt tgattttttt ctgaatagtt tcagtcagaa tagtaccatc 76621 tcttctttgt acctctggta gaattcatat ggtcctgggc tttttttttt ggttggtaag 76681 ctatttatta ctacctcaat ttcaaaatgt gttattgttc tattcaagga ttcaatttct 76741 ttctggttct catgtttggg ggcttatctg tcttcagtct ttgaggttgc tgacctttgg 76801 atgtggggtg tgtgtctgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg gttgttgttg 76861 ttgttcattt ttaacagttt ggccactctc cagtagggct gctgcagttt gctgggggtc 76921 tgctccagat cctagtcatc ttggtttttc cagtacctgg aggtatcagc agtgaaggct 76981 gtgaaacagc aaagatggcg gcctgcccct tcctctggaa gcttcctctc aggggaatac 77041 tgacttgttg ttgggccgaa tgcacctgta ggaggtggct ggagaaccct attggaaggt 77101 ttcacccagt taggaggaac gggatcaggg accttcttaa atatgcagtc tagctgcatt 77161 ttggtggaac agttgtgctg tgttggggat cccttcatcc cccaatcgat ttgggctctc 77221 caatgcctgc aggctggact ggctgagaca cccaaacagc aaaggtggca gccagcctcc 77281 cacacccagg catttcattc atcccaggga gaaattagaa ctctgcaggc tgtagaacat 773-41 gggtgggggt ggctggaggc cccagctggg aggatcagtc cagtgagaaa aaatggatct 77401 ggatcctgtt taaagcagca ttctggccgt gcccacaaaa cagccatgct gtgctgggaa 77461 accacctctg ccctggtctg ctgggactct ccaaagcctg caggctgaac agcgcagtcg 77521 tccaaacacc aaaggtggtg tcccacccct cccccagggc actccatccc agggagagat 77581 cagagctctt tctagaatac tggcaggtga gggtggctgg aggccctggc tgggaggtcc 77641 cgccccatga agaggaacag atgagtcctt aaagaagcag tctgaccaca ttctggcaat 77701 gcagttgtgc tgcactgggg gaacgcttcc tcagactgtt tggactttct aaagcctgca 77761 ggctggaagg gctgagttga ccaaacagct aaggtggcag cccacccctt cccctgggga 77821 ctccatccct tctcaggcag gctctaccct gttgccagtg gctggctgga gttccaagcc 77881 agtgtgtctt atcttgtgag aagctgtgga agtgggcctg cagactgacg ctgctcagaa 77941 ccctggattc agccctcttc ctagggctgg atttttagtt tcaggtctta aatatattta 78001 agtctttaac ctaagttaat ttttatatag gtgtgagata aggatccaat ttcattcttc 78061 tgcatgtgga tatctagtgt tcttgatgcc acttattgaa gagattattt ttctccattg 78121 tgtgttcttg gtacctttat tgaaagtcaa ttatttgtaa aagtgtgggt ttacttttgg 78181 acactatatc tggtctcact ggtcaatgtg tctgcttttg tgataatata ctgttttgat 78241 tactgtactt ttgtagtata ttttgatgtc aggcagtatg atgccttgag ctttgctctt 78301 tttgcttatg attgttttag ctactgaggt cttttgtggt tccatataac ttttaggact 78361 tttttttcta tttctgtgaa aaatattatt gtaattttaa taggaatttc attgaatcta 78421 cagattacat tgggtagtct ggatacttca atatttttcc aatttatgaa tatgggattt 78481 cttccaattt gtgtttttgt tcaatttctt tcacctaagt ttttgttgtt gttgtgcaat 78541 tattYaacac tcccactttt aacagtagac accctgtcag cctggaagtt tgctttaact 78601 cagctgactg catgatgagg tctagtgttt attttcagca attacagcct tgtgSctcta 78661 ggagatcaga ttctctctca gggcacacta gaagctctta aatcatttat gcattgcctg 78721 agccagaaat tcaaatccct gagctcatcc ttttaaaaat tatgttgtcc accaacatta 78781 gggataacca actgacataa ttatattcct taagttttcc acaagttttg aaaatgtcat 78841 acacagaatc actaaattac atgcctctta caagtggttg atcaggagta cccaatccag 78901 atattttgtg tatcacaata agcagttcat cccatagact atcagtgttc cctgtactgt 78961 tggaaataag actccttagc acttgaagtc caatcagatt agaaagcaaa ttctagaaac 79021 cccaaaacca attaaagaaa ctcatcctaa aagttctgtt cctctagMat aactcctggt 79081 gccacaaatc tctattagtc agggttcttt agagatgtag ataaagattt atcatgggaa 79141 ttggttgaca catgatggaa gggcataagt cccatgatat gtaatgtgca agctggagaa 79201 atgaaagcca gaggtgtagt gctgtccaag tccaacagcc tgagaacctg ggggacactc 79261 gtttaagtcc tggagtctgt aggctagata acttacaatt ctgatgtcca aaatcagaag 79321 aagggtgtct tagctccaga gagggcaaat ttgtctttcc tctgcctttt tgttctatat 79381 aggccctcaa ccatttggat ggtgcctgtc cacattgtgt gaggatggat cttccttact 79441 tggtctactg attcaaatgc caatctcttg atcaacaatt cacagacata cccagaaaga 79501 atgctttact agctctctgg gtatctcata atctagtgga ggtgacatct aaaattaacc 79561 atcacactag gagaagatag atcacttctg gagcttgaat ctttataaaa acagtgagat 79621 gtatccatct tctgtgttct catcctggcc cctaagtgca atgattaagg aataggatgt 79681 ggctcctggg tttttagttg taacaaatag cttttagagt acttatggat ctttatatat 79741 agaactgaag acctgccatg tatatagctt atttcaaaat agaatataga atcgtcatca 79801 aaataggata tacctttcct gaaagtaatt atatgatgtt tgcatgtccc tgtgttccag 79861 aaccagacat tgcttgcatt tggaatgaat ggcccataga tagtcaaaag aacaaaagaa 79921 aggatgggtg cagctataca ttaataggct tacagataaa aatacacaaa acacactcaa 79981 taaaataaat tatttataac ttaaaatttc ttcttttacg taaaaaatag caaactgtaa 80041 tacttttact accctctagg catgtggtta ctgagcactt gaaatatggc tagtccaaac 80101 tgcgatgtgc tgtaactgta aaatgtattt tgaagattta gtatgacaaa atgcaaaaac 80161 tcaatttttc tatcaatact ggtaatattt agatagatta tataaattcg tttcacttgc 80221 tttttacctt tttatttttt aatttttttt ttttttttac aggcagggtc ttactctgtt 80281 ccaggctgaa gtgcagtggt gcaatcatag ctcactgcag ccttgaactc ctggactcaa 80341 gtggtccttc tacctcagcc tcctgagtag ctgggactac aggcacatgt caccacacct 80401 ggctaattaa aaaatatttt taagagatgg gggctttcct ataccatggt gtggtaactt 80461 cctataccat ggtggaatga aattagaaat caataccaaa aggaactctc aaaactgcac 80521 aagcgtggcc aggcatggtg gctcacacct gtaatccaag cactttggga ggccgaggtg 80581 ggcagatcac ctgaggtcag gagttcaaga ccagcctggc caacatggtg aaaccccatc 80641 tctactaaaa atataaaata ttagctgggt gtgatggcgt gcgcctgtaa tctcagctac 80701 ttggaggctg agacaggaga atcgcctgaa cccaggaggc ggaggttgta gtgagccgag 80761 attacaccat agcattgtgg cctgggtgac ggagtaagac tccgtctcaa aaacaaacaa 80821 aaactgcaca agtacataaa aacaaaacag cttgctcctg aatgactcct ggggaaacaa 80881 tgaaattaag gcagaaataa gaaaattctt tgaaataaat gaaaatagag acataacata 80941 ccaaaacctt tgggatacag caaaagtaat gttaagagga aagtttatag aataaatgcc 81001 tacacaaaaa gacagatctc aaattaacaa cctaacatcg cacctcaagg acctagaaaa 81061 acaacaaact gagttcaaat ctagaagaag ataagaaatt acaaagatca aagcagaact 81121 aaatgagacc aaaaaatgag agaggatcaa tacacaaaaa gttggttcat tgaaagagta 81181 aaccaaattg acagctagat taaccaataa tgagtaaata aatcaaattg gtaataaaac 81241 atcttccaaa acaacaaaaa gcccaggatc agatggattc acagcgaaat tctaccagac 81301 atacaaagag ctagtatcaa tcttattgaa actattccaa aaaaactgaa gaSgaaggat 81361 gcctcccaaa ctcattctat aaatacagtg tcaccctgat accaacaatc agtgaaggat 81421 acaagaaaaa aagagaactg caggccagtc ttcctgatga acatagatgt gaaaatcctc 81481 agcaaaatac tagcaaacca aatccaacag cacatccaaa aaaaaaaaaa aaaaaaaaaa 81541 agcatcatga tcaagtgggc tttattctag gtatgtaagg aggatgattc aacatacaga 81601 aatcaataaa tgtgattcac cacataaaca atagaacaaa aactacatga tcatctcaat 81661 agatgcagag acaaacacca gataaaatct aatatccctt catgatgaac accctcaaca 81721 aactaggctt tgaaggaaca tacctccaat aataaaagcc atatattaca aacctacagc 81781 caacatcata ctgcatgggt taaagttgaa agcatttccc taagaactgg aaaaaagacg 81841 agaatgtgca ctctcaccac gtctattcaa tacagtattg gcagtcctag ccagagaaat 81901 cagacaagag aaagaggtaa aagacatcca aattggaaaa gacaaagtca aataatctgt 81961 tttgctgatg acacgattgt atacctagaa aaccctaaag actcctccag aagactcgta 82021 gacttgacaa gtgagttcag taaagtttca ggatacaaaa tcaatataca aaacccagtg 82081 gaatctctat acaccaataa cattcaagct gagaacaaaa ttaagaactc aatcccattt 82141 ataatagcta cacacacaca cacgcacaca cacagcacct aggaatacat ttaaccaagg 82201 aggtaaaaaa gaactacaaa gcacagaaga aactgcagat gacacaagca aacggaaata 82261 catcccatgc ttatgaactg aataaccaat atgattgttt taaatgacca tattgcccaa 82321 agcaatctac agatttgatg taattcctat taattaccat cattttttca cagaactggg 82381 aaaaacaatc ctaaaattca tattgaacgc ccaactccaa aaaaaaaaaa aaagcctaaa 82441 tagccaaagc actcctaagc aaaaagaaca aatacagagg catcacattg cttgacttca 82501 aggctatagt aactgaaaca gcatggtagt ggtataaaaa tacacacagg tcaatggaac 82561 ggaatagaga actgagaaat aaagccacat acttacaact aactgatctc tgacaaagtc 82621 aacaaaaata tacactgggg aaaggacacc ctactcaata aatggtgctg ggaaaactga 82681 tttgccttat acagaagaaa taaactagac ccttacctct catcatatac aaaaattaat 82741 gcaagatgga ttaaagacct aaatgtaaga cttaaaagta ttaaaatgct agaagaaaac 82801 ctaggaaaaa ctcttcagaa tgttggcctc agcaaaaaat tcacaatgaa gaccctaaaa 82861 gcaaatgcaa caaaaccaaa aatagataaa tggaactgaa ttaaaattaa aggcttctgc 82921 acagcaaaat aatcaactaa ataaacagat aacctacata atggaaaata tatgcaagtt 82981 atttctctga gataggacta atattcatca ggtacaagga actaaaacaa taagaaaaac 83041 cccaaacaat cccgttacaa actgggagaa aaacatgaac agacatttgt caacagaaat 83101 acaactgacc aacaaacacg tgaaaaagtg gtcaacgtca ctaatcatta cagaagtaca 83161 aaaaccacac tgagatacca tcttatacca gtcacaatgg ttattattaa aaagttgtca 83221 aacaacagat gatggtgtca atgtgtagaa aagggaacat gaatactctc ttggtgggaa 83281 tgtaaattaa ttcagcctct atggaaagca gtatagagat ttctcaaaga actaaaaata 83341 gaactaccat tcaacccagc aatctcatta ctgggaatct acccaaagga acagaaatca 83401 ttatataaaa aagatatctg tacatgtatg tttattgcag caatatttac aatagcaaat 83461 tcatggaacc aacctaagta tctaccaaca attgactgga taaagaaaac gtgagatata 83521 tatagagaga gatatataaa tggagtatta tgcaactata aaaaagaatg aaatcatgtc 83581 atttgcagca acatggatag agctggaggc cattatccta tgtgaactaa atcagacata 83641 aaaaataaaa tattgcatat attctcactt aaaagtagga gctaaacaat gggtacacaS 83701 agacataaag atggagataa cagacactgg ggactccaat aggggaaaga gtgtgagcgg 83761 cagtaagcgt tgaaaaatta cctgttgggt acagtgttta atatttggat gatgggtaca 83821 ctagaagccc aattcccacc attattcaat acacccatgt tacaacaagc acatgtaccc 83881 cctggatcta aaataaaaat tttaaaaacc tcacattatc aataaaaatg tggcggagta 83941 tgttcaattt tcttttcttt ctatttctag catacaagtg agcccttctc tgctataaag 84001 gaaactgtcc ataaatcact taaaatgaga ctggttaatg atttttagta aaatacacca 84061 ttatatgttt tttttcttat tggttaaagg caatttattt tgaaatgttg ctttggttgt 84121 ttgctttctg gaaacatatt ggaacacttg tttttcataa gcYgtcctga cagtggcaca 84181 atcccatcca tcttcaggcc ttttaataag gtcattatga aatctgaatt tctattaata 84241 ctctggtgca ttcatttcat ctgcaaaagc aactggcaca accactcctt gccggtgcag 84301 ctctcggaga acatctaata ttgagtctag ttctgtgcgg aacttctcca gctcacgatt 84361 ctttaactgt gccagtcttt tccatttttc aacttctttg ttttgctcag tttctactac 84421 ttggtgtgtt tgctgtatta tctgcaaata caaaaattcc acattgctgt tatagttcat 84481 agtggtgggt gaggaacatt taacaatctt aaagtaggca aacctggggt ttctttgggg 84541 aactcaggta aatcataatg ctctgcgtgg atgcaacatt ttctgtcttc aaggagtata 84601 tacatcccaa ctgtgttagg gactaaatgt ttgtgtcccc ctcaaattca tatgttgaat 84661 ttcctaaccc ttaatgtgac tgtatgtgga gtaaggaaat aatgaatgtt aaatgagatc 84721 ataagggtgg ggacttgatt tgataagaac cagtgtttcc accactagag acaccgaaga 84781 gatcatcacc agaagagaca ccaaagtgat ctctcgcccc tcttcctctc ctctgcacac 84841 atggtactga ggaaagatca tgttagaatg agaaaaaagg tggctatctg caagctggaa 84901 agagagccct caccagaaac ctaactgacc aaaacctcaa tctgggactt ctggcctaca 84961 gaactgtgag aagataaatt tttcctgttt agccacccaa tttatgatat tttgttatgt 85021 cagcccaagc agactaatac actgttcaaa ccaagttaaa tttcagcacc aaggcaagtg 85081 cctaaaaaca catcatgccc ttacatctgc tatttttaac ttctattaat ttatctgttt 85141 ataattaagt atagtaatat tactagtgct agctatatat tttaaataat catattaaaa 85201 caatttattt cttataaaca tttgctgaaa tgacttgaat atggaacacc atgccttgtt 85261 cccagggtaa gatgtgtaag tttttaggaa tccatcaata attaagaaca tgatacaagt 85321 ttttgctttt tttccagata taaggatacc aatcttcaag cacttgagat ataaagtctc 85381 attttctggt aaaagttata aatttattaa atttttaaca agatcaaaat acactggaaa 85441 cactttaagt ggtaattttt gctagggatg acaaaactat agcaaattaa aggcacttaa 85501 aagtaaagac ctatataaat atgtaaatgt gatttacttt acctgttgaa gttcctgttc 85561 tctttgtgca tgtctcattt ccatctgctt aattttcttt tctaagccca cgaaatgttt 85621 catctctggt gtatggtttt ctttggcttc tctcaattct tctaagagtt ttgtaatcta 85681 aaattgacat atgaagcaaa tgaaaaacta taatcacaag aatttaacag gtaatcttga 85741 aaatcagaat atttgtataa aatttctcta tataagtaag aggcaccttg gattgttaga 85801 gtaattaata gctttcattc tctatatcca gaaagtaaag gttcMtccta actctgagtt 85861 gacctcacat tctaagaggt tccggatgaa tggaccatct tctacctccc aagtatggca 85921 gtctggtaaa ggagctgaaa atgaagttgg cactttcttt aatgcattaa gtttcactac 85981 aaggtattgt tagtaagatg gttactttca gaatttaaaa aagtacattt aaagcactca 86041 acatagtaca gtgccccatg cacagaaaga gttcaatgaa tatgtatttt tcttctgtta 86101 actccctata attctatttg taattctcta tggcccttat cactttataa tttgtagtag 86161 ttcattccac aaataaatgt atacatataa agtagaaata atgaagtact gagccctttc 86221 tgtgttgact gctgtcttag gacctgggga tccagagatt aaagacacag accccatcct 86281 cacagagtgt agtgcagtgc cttcatattg agcttgggaa aacaattaat tgtatcttct 86341 ttatcccata taatcatgaa aagaaattcc aacaagaatc agattttgct aattgtttac 86401 atcaaaaggc aacaaaaacc aaatgaaaat ggatagaact aacaataact cttgttttct 86461 atgaaaacac ctattaataa caataatact aatactaaaa aatcccagta tcttacctac 86521 attagttgaa aaacatgcaa agatacaagc aaattaaatc agaggcaatc tattttaaat 86581 gtggagcatc cctgatgata tcatcattga acaaagaact atacagacta taattgggat 86641 ggtttgcaga gagctcaggg agaggcctca cagaacatgc tgtggtgatg agtgttggtt 86701 gggcataaaa actgtatgtg ttaaaataat gattattaaa attatttttt aaaaaactaa 86761 tgactaaact aagaaagctg ataaaggcat agtcatacac agaatggtca attattattt 86821 caattcctgt aaccttttta atggttgaaa aatgattttg agtaatattt ccagctgtta 86881 ctatcttgtg atacacctgt tcctggtaca ccagttagga agctctggtt ttctgaatga 86941 aacagtcaag tgaatgagaa ttctaatatt gtgtgctatg ataaagggat ggccaatgac 87001 tagtgagata agtgagaaca cctgactgga agagaagaag ggccacagag aattcctaag 87061 gtggctgtgt ttgaattgag tcttaaaggg taagtagtag tcagtcatta tattatgatg 87121 tgctaggcat taggctgagc actttacaga tgtctcattt ttttattaag ccttatatat 87181 gtttactgtc tgactcctca cataaaatgt aagcttcata agagcaaaga tttttgtctt 87241 tgttcacctc actattccta gcacccagcg ctgcccatgg cagagaggta ggtgtttaac 87301 aattattcat gaattaatta gtccattcac ctttcaatga ttaaacattc attggtaaat 87361 cacataaact tagaaatctg gttttagaca aatagagaaa atttcctttt aattgcacct 87421 acctaaaaga acctttaaga atataatcaa aactgaatat gtacatatat tttaaaaatc 87481 cttgcatttt aatgaattat cctctagaga taactgaata cttaataagc aaacatgtat 87541 ttaaaaaata cttcctgcta tgtacacatt atttaaaaaa actggaaaca acctgcatgc 87601 caacaatagg ggatttgtga aataaattag taatgattaa taagagcctg agacctagag 87661 tcagaggcaa tgggttctaa cggtggcact tctatatggt ggtcggtaag ccaccatata 87721 gaagttattt cactttcctt agcatcaata tcttcatcta aaatgggaat aatagtagta 87781 actactcttt tggttgtgat gagaatcaaa taaaataagg tatataaaac actttgcaca 87841 gttcctagta aacattaaac tttttttttt aagttatact ttgttctggg gtacatgtgc 87901 agaacgtgca ggtttgttac ataggtatac acgtgccatg atggtttgct gcacccatca 87961 acccgtcatc tacattaggt atttctccta atgctatccc tcccctaacc cccaaccccc 88021 cgacaggccc cggtgtgtga tgttcccctc ctatgtcact gtgttctcat tgttcaactt 88081 ccacttatga gtgagaacat gcggtgtttg gttttctgtt cttgccttag tttgctgaga 88141 atgatggttt ccagcttcat ccatgttcct gcaaaggaca tgacctcatt cttttttatg 88201 actacatagt attccatggt gtatatgtgc cacattgtct ttatccaatc tatcattgat 88261 gggcatttgg gttggttcca agtctttgct attgtgaata gtgccacaat aaacatatgt 88321 gtgcatgtgt cttaatagta gaatgattta taatcctttg ggtatatacc cagtaatggg 88381 atggctgggt caaatggtat ttctagttct agatccttga ggagtcgcca cactgtctcc 88441 cacagtggtt gaactaattt acactcccac caacagtgta aaagcattcc tatttctccg 88501 catcctctcc agcatctgtt tcctgacttt ttaatgatcg ccattctaac tggcatgaga 88561 tggtatctca ttgtggtttt gatttgcatt tctctaatga ccagcgatga gctttttttc 88621 atatgtttgt tgactgcata aatgtcttct tttgaaaagt gtctgtccat atcctttgcc 88681 cactttttga tggggatgtt ttttttcttg taaatttgtt taagttcttt atagattctg 88741 gatattagcc ctttgtcaga tggatagatt gcaaaaattt tctgccattc tgtagggtgc 88801 ctgttcactc cggtttttgc tgtgcagaag ctgtttaatt agatcccatt tgtcaatttt 88861 ggcttttgtt gccactgctt ttggtgtttt agtcatgaag actttgccca tattgatttt 88921 ggactgagat gatggggttt tctaaatata caattgtgtc atctgcaaac agacacactt 88981 tgacttcctc tcttcctatg tgactaccct ttatttcttt ttcttgcctg attgttctgg 89041 ccagaacttc cattactaag ttgaattgaa taggagtggt gagagagggc atccttgtcc 89101 tgtgctggtc ttcaaaggga atgcttccag tttttgccca ttcagcatga tattggctgt 89161 gggtttgtca taaatagcta ttattttgtg atacgttcca tcaataccta gtttgttgag 89221 agtttttagc atgaacggct gttgaatttt gtcgaaggcc tcttctgcat ctatagagat 89281 aatcatatgg tttttgtcct tggttttatg tgatggatta tgtttattga tttgcgtatg 89341 gtgaaccaga cttgcatctc agggatgaag ctgacttgat catggtggat aagctttttg 89401 atgtgctgct ggatttggat tgccagtatt ttattgagga tttttgcatt gatgttcatc 89461 agggatactg gcctgaaatt ttcttttttt gttgtgtttc tgccaggttt tggtatcagg 89521 atgatgctgg cctcataaaa tgagttaggg aggagtccct ctttttctat tgtgtggaat 89581 agtttcagaa ggattggtac cagctcctct ctgtacctct ggtagaattc ggctgtgcac 89641 ccatccagtc ctggactttt tttggttggt aggctattaa ttactccctc aatttcagaa 89701 cttgttattg gtctattcag ggattcgact tcttcctcgt ttagacttgg gagggtgtat 89761 gtgtccagga atttttccat ttcttctaga ttttctagtt tatttgcata gaggtgttta 89821 tagtattctc tcatggtagt gtgtatttct gtgggatcgg tggtgatatc ccctttatca 89881 ttttttattg cgtctatttg attcttctct cttctcttct ttattaattt ggctagtggt 89941 ctattttgtt gatcttttca aaaaaccagc tcctgaattc actgattttt tttgaaaggt 90001 ttcttgtgtc tctatctcct ttagctctgc tctgatctta gttatttctt gtcttctgct 90061 agcttttgaa tttgtttgct cttgcttctc tagttctatt aattgtgatg ttagggtgtt 90121 gattttagat ctttcctgct ttctcttgtg ggcatttagt gctataaatt tccctctaca 90181 cactgcttta aatgtgtccc agagattctg gtacgtcatg tctttgttct cactggtttc 90241 aaagagctta tttatttctg ccttcatttc attatttatc cagtagtcct tcaggagcag 90301 tggttcagtt tccatgtagt tgtgcagttt tgagtgagtt ttttaatcct gagttccaat 90361 ttgactgcac tgtggtctga gagactgttt gttatgattt ccattctttt gcatttgctg 90421 aggagtgttt tacttccaat tatgtggtca attttaaaat aagtgtgatg tgctgagaag 90481 aattctgttg atttggggtg gagagttctg tagatgttta ttaggtctgc ttggtgcaga 90541 gctgagttca agtcctgaat atccttgtta actttctgtc tcgttgatct gtctaatgtt 90601 gacagtgggg tgttaaagtc ttccattatt attgtgtggg agtctaggtc tctttgtagg 90661 tctctaagga cttgctttat gaatctgggt gctcctgcat tgggtgcata tatatttagg 90721 atagttagct cttcttgttg aattgatccc tttaccatta tggccttgtc tcttttgatc 90781 tttgttggtt taaagtcttt tttatcagag actaggattg caaccgctgc tttttttttg 90841 ttttgttttc catttgcttg gtagatcttc ctccatccct ttatcttgag cctatgtgtg 90901 tctccacatg agatgggtct cctgaataca gcacactgat gggttttgac tctttatcca 90961 gtttgccagt ctgtgtcttt taattggagc atttagccta tttacattta aggttaatat 91021 tgttatgtgt gaatttgatc ctgtcattgt gatgttagct ggttattttg ctcattagtt 91081 ggtgcagttt cttcctagca ctgatggtct ttacaatttg gcatgtttta gcagtggctg <$ 91141 gtaccagtcg ttcctttcca cgtttagtgc ttccttcagg agctcttgta aggcatgcct 91201 ggtggtgaca aaatctctca gcacttgctt gtctgtaaag gattttattt ctccttcact 91261 tacgaagctt agtttggctg gatatgaaat tctgggttga aaattctttt cttgaagaat 91321 gttgaatgtt ggcccccact ctcttctggc ttgtagagtt tctgctgaga gatctgctgt 91381 tagtctgatg ggcttccctt tgtgggtaag ctgacctttc attctggctg cctttaacat 91441 ttttttcctt catttcaact ttggagaato tgataattat gtgtcttgga gttgctcttc 91501 ttgaggagta tctttgtggt gttctctgta tttcctgaat ttgaatgttg ttctgccttg 91561 ctaggctggg gaagttctcc tgcataatat cctgaagagt gttttccaac ttggttccat 91621 tctccccgtc actttcaggt acaccaatca gacatagatt tggtcttttc acatagtccc 91681 atatttcttg gaggctttgt tcatttcttt ttactctttt ttctctaaac ttctcttctc 91741 acttcatttc attcatttga tcttcaatca ctgataccct ttcttccact tgatcgaatt 91801 ggctactgaa gcttgtgcat acatcacata gttctcgtgc catggttttc agctccatca 91861 ggtgatttaa ggtcttctct atgctgttta ttttagctag ccattcgtct actctttttt 91921 caaggttttt agctcctttg cgatggtttc aaacatcctc ctttacctcg gagaagtttg 91981 ttattactga tcttctgagg cctacttctg tcaactcgtc aaagtcattc ttggtccagc 92041 tttattccaa aggaacacag ctggcaagga gctgtgttcc tttggaggag aagaggcact 92101 ctgatttttg gaattttcag cttttctgct ctgttttctc cccatctttg tggttttatc 92161 tacctttggt ctttgatgat ggtgacctag atatgtggtt ttggtgtgta tgtcctttat 92221 gtttgttagt tttccttcta accgtcagga ccctcagctg caggtctgtt gggagtttgc 92281 ttgaggtcca ctccagacac tgtttgcctg ggtatcacca gcagaggctg cagaacagca
92341 aatattgcag aacagcaaat gttgctgcct gatccttcct ctgggagctt tgtctcagag
92401 gggcacccgg ctgtatgagg tgtcagttgg cccctattgg gaggtgtctc ccagttaggc
92461 tactcaagtg tcagggaccc acttgaggag gcagtctgtc tgttctcaga tctcaaactc
92521 catgctggga gaaccactac tctcttcaaa gctgtcagat agggacgttt aagtctgcag
92581 aagtttctgc tgccttttgt ttggctatgc cctgccccca gaggtggagt ctatagaggc
92641 aggcaggctg gcctccttga gttgcggtgg gctccaccca gtttgagctt cctggccact
92701 ttgtttacct actcaagcct cagcaacggc ggatgcccct cccccagcct tgctgctgcc
92761 ttgcagttca atctcggact gctgtgctag cagtgagcaa ggctctgtgg gcattggacc
92821 ctctgagcca tgcgcgggat ataatgtcct ggtgtgctgt ttgctaagac cattggaaaa
92881 gcacagtatt agggtgggag tgtcccgatt ttccaggtac cgtctatcac agcttccctt
92941 ggctaggaaa gggaattccc agaccccttg cgcttcctgg gtgaggtgat gccctgccct
93001 gcttcggctc acactccttg ggctgYaccc actgtccaac aagccccagt gagatgaacc
93061 tggtacctca gttggaagtt cagaaatcac ccatcttatg agtcgctcat gctaggagct
93121 gtagactgga gctgttcctc tttggccatc ttgcatttca gatgggggtt ttttctataa
93181 gaccctgact ggggctgctg cctttaagag atgccctgcc cagagaggag gaatctagag
93241 aggcattccg gctatggcgg ctttgccaag ctgcagtggg ctctgcccag ttcgaacttc
93301 ctgtaggctt tgtttatgct atgaggggaa aactgcttac tcaagcctca gtaatggtgg
93361 atgccactcc ccccaccaag cttgagcatc ccaggtcact tcagacagct atgctggcag
93421 tgagaatttc aagccagtgg atcttagctt gctgggctct gtgggggtgg gaatctgctg
93481 agctagtcca cttggctccc tggcttcaac cctctttcca ggggtgtctt gctggcattc
93541 caggcgccac tgtggtatga aaaaaaactc ctgcagctag ctcagtgtct gcccaaacgg
93601 ccgcccagtt ttgtgcttga aacccagggc cctggtggcg taggcaccca agtgaatctt
93661 ctggtgtgtg cattgcgaag accttgggaa aagcatagta actgggctgg agtgcaccgt
93721 tcctcatggc acagtccctc acggcttccc ttggctaggg gagggagttc cccgacccct
93781 tgcacttccc ggatgaggca aggccccacc ctgccttggc tcaccctctg tgggttgcac
93841 ccactgtcta accagttcca atgagatgag ccaggtacct cagttggaaa tgtagaaatc
93901 aStttccttc tgtgttgatc tcactgagac ctgtagaata cagctgttcc tatttgacca
93961 tcttgccagc ctccccccca cagattaaac ttttgataaa taattatatt ataatgcctc
94021 tattttgaaa tggtataata gaagtgccaa taaagatata tactgtcatg aaaagatcat
94081 atatacacac acacacacac acacacacac acacacacac atatatagtg ttaggttaaa
94141 aatgctttga aaatcatatg catgcataca tacatacatg aacaaacatg tatttgcata
94201 tgtacacaca tgagaaccat atccacaata tttgtattta tttatgaata gaaaaaaatc
94261 tggaaggata tagagcaaaa tataactagg tagtgggatt atagtgattt tataccttct
94321 aattgctaat actaattatt atgtattaat agtataaaaa cacatgcata gtgaatccat
94381 gctcaactaa ggacagaaat agcatcaata aaaagcataa aagcatatat tctttaatga
94441 ataaaactgt tatcttttta ttactttact aaagtaaaaa agagtgattc acccagatga
94501 tgtcacttaa aggtgagtga agatgctttc ttactggaga agacagatat cactgactgc
94561 tggcaggagg tggctcaggt gctggagttc aaacaatctg tgcgtagccc agagacagac
94621 tgcttcccag agaagacatt tgtctttgtt ctcttacttg ttggtgagta atcttagaaa
94681 ctacacatct caatttaaac tgaaccacaa aattattcct atttttatta ctttaaacaa
94741 gcctgggctg gtcgtggtgg ctcacgcctg taatcccagc tctttgggag gctgagacgg
94801 gtgaatcact tgaggtcagg ggttccagac cagcctggcc gacatggtga aactccatct
94861 ctactaaaaa cacaaaaatt agccgggtgt gatggcacaa tcctgtaatc ccagctactc
94921 aggaagctga gacataagaa tcacttgaat gagggaggca gaggttgctg tgagccgaga
94981 ttgtgccact gcactccagc ctgggcgaca acagtaagac tgtctcaaaa aaacaacaaa
95041 aaaatgagcc tggtacactg tatctgtgta cttttcaatc atgtcatgtg ccttcgtgct
95101 ttgtaactat tggcataagt cttcctggtc actttgctag aggaattgtg agtacttact
95161 taatYtagag ggtttagatt ctaattaggt tagcactgaa ggagatcctg ggcactgtgt
95221 cttaagcagc cattcctgct ttMtttgctg acaaggcctt gatattgttc aggtgttcat
95281 gtgatgctgg ggtaaattct gatcagtttt actgtcagtt tactggataa ctactggaca
95341 aaaacaactg atcagttata gctgtccagt ttttcttggY agtgattggt ttagaggtgg
95401 gcacatgYct tagtctgttt tctgctggta taaccaaata ccacagacat ttatttggct
95461 tacagttcgg catctggaca gtccaaatgc atggcactgg catctgggaa gggccttttg
95521 ctgcattaac ccatgacaga agggcaggca ggtgatgaga cagagaggga atgagaacca
95581 ctgaacttat tcttctatca tgagcccact cctgtgataa ctaacccatt cctgcttgaa
95641 catcaactaa atttaaacat aagttttggc agggatattc aaaccgcagc agcatgtgac
95701 acagttctga agcaaaggca gttagtggga gacattatag gctatatgta tctgtttgat
95761 tatttttctg tcccactggt tagaatgttc cttgacagca aggactccag atgtttcact
95821 gctatatctt aagaagcaaa taacagggac tgacatctgc tcactaagta tttattaaat
95881 caatgaaaaa aaatgctatg aaggtcttat ttaacttgat agctgtaaaa actgaggctg
95941 agagagttta aagacactag aagtggctat gtacagataa cttttttctt ctgcatgaga
96001 aactgacctt gggttcaatc acttttctgt gtctgttctc cccttgataa agaatggcca
96061 tcggccacac accaggcacc taggcttggg ggactttgta tgaactgaaa tacttcatag
96121 gaagacagtt ttgggctctc ctgaccatgc tgactcttgt aactgagcat gttgttgtgg
96181 gagccaccgt gcaaggatta gtataagaga tatcggttct tgtggagcat gcggcatcta 96241 aactagcaag ggaataagag tcatacctgg caggtggtat gagtcttatt tccttgcacc
96301 tttgttgtca cttggacagc tcctggacaa ctatatgaac tgctgtgaga atgacttgca
96361 ttgaagagaa atgcgtcatg ctgccttgct agcctgctgt ttcctggcct gtatccagca
96421 tgccaagtgt ggccttatgg ttgttgtgtg actctaaaYg tttacttgaa catgctagcg
96481 agccttacaa cggcaagtca agaatttaaa cccagatctt ataaacccta tgatcattcc
96541 actatctgtt cataaaacaa cagcagctag ctgtagaata tttgcgacaa attacagtaa
96601 gaattttgat aattcttatg agtgggccac aatagtccct ggggtcttat tagaggtaag
96661 tcttaaatat tttctatatc ttttataaaa tatgcagatt aaaaaaaata aaaccccaca
96721 aatgaaaaaa tctgtaatca gtaaaatgta tatcctaata atagaaaaaa tatgaaaata
96781 agcaataata gcatcaatcc ttagttatcc atagggatca tgagtaaaga ttcatgtctt
96841 ttctctacaa ctgcagggtg tgaagaaata aatgaactga agatttttgc agtcaattag
96901 aagtggaaca atagattgaa aaatctatga tgaatactaa aacacaaaag aatattaaaa
96961 ctctagaata ttaaaactct agagtcttaa aaaaacccaa ggatttgaaa aatacatgcc
97021 tgagttagca tcaacggaag aggtgctgga tccttaataa gattttcacc ccattgcagc
97081 agacataatt ttaaaagatg tgcttgaaaa tattttaggt aacaaaggca tgaaacataa
97141 tgaagagttt agaaacatcc tttcagattt ctataattaa tatactaagt gggaatcatc
97201 tttgctttat gctgaaaact tataaatcat tgctcagtta tctcatggcc tataaaatgt
97261 taaaaacaca taagcaatca aaagttcacY aatggtttaa aatgtatagt gtgctttcat
97321 ctgtggcgat gtatagtgta ctttcacttg tggtcatgtg gagtcaaggg gagactcctc
97381 ctttaaataa ctagaaaaca gcacaaaata tttgaaacaa ctgttttcag acactggata
97441 acagcactac agactgtgat ttctgggaga agggaaataa ggtgagctct agagttgtcc
97501 agtttattgc ctggaggcag ttttgaagcc agtggaaata gcctgagcat ttcctggagc
97561 tcacacagag gtgggaatag ttcatatttc caccaccctg agaggaaatg cctcataata
97621 cacaggacgt caggagagtc ctcaaactag tcttgtcaaa acagctagaa tattacacag
97681 aatacagcca ttataaataa actccatatt aaaaggtagg tattccaatt aaaaggcagg
97741 gtgtcattac ctttactcag gacactgagt gcttagcttt ctagtagtga ggcttaaaaa
97801 tcatgtgctg cgtcttctat tttcaagtat tttagatatt cttaaagtaa gaactgtctt
97861 accattgtat tgctaaatta acaacaatta agttaactaa aactgacttc attatcttgc
97921 aaacaggtac atattctttc ttttattgct ttcactaaaa actttacttc agttctgagg
97981 ttctgtaaac ttagtgctta tgttaacaaa gttttatgta catcttaaac actgaaaaga
98041 atgcacaatg ctgaactagc ctttcaacta ctagaacaag attttaaaaa ctgacacttg
98101 cgtaattgta gagtgttttt acattgatct tttcataata caattggctc tatattttgc
98161 ctgtgagact taaatccttc aataacaata aatggtatat atgcatcaaa gaacgggatg
98221 tgcagctttc ctggcctttc acacttttta gaatgaaatg gatcttagaa tcaaatggaa
98281 gaaaagctct ctatcctcct ctttttctgc ctaaaagtag gaaatacatt ttcctttagt
98341 gaagagggct ctagatgctt attagcccag agatggcacc agaggaatct gcagtactcc
98401 atgagtttcc taccatatat ttacattccc acagtttccc acctttgtag cctaaaaacg
98461 actttcctct gtcctttctt tatctacaaa ttgattgttc tttgttgaag atggtttgta
98521 aaccagagtt ctaagccact gttttgagtt acttttcatg aggtttctcc tgtgatgtgt
98581 gctgcacaca ttaataaatt tgtttttctc ttgttaatct gtcttttgtt acagtggcct
98641 gtcccaacag gaactcacat gggttgagaa aaaaaatttt tccttcctga cagtttttgg
98701 caatgcagat gggactttct gggatacccc atccactctg gaacctgcag atgaaatcct
98761 agcaaaactg gcagaagctg gtaaaaggta agaattctta ccaaactcag ctctcccaga
98821 tatctgtcta tagcagtcag tcaagaaagg aaggtaaaat ttttoctggt ccttcttttc
98881 catattcaga ttggtaggag aaaaaaattt gtaaaattct ttgaatttgt gactcttgtg
98941 ggtttggttt tggctaccta ttggttatta tttttagcct ccttgaaaag gtcattgttt
99001 tccattgtct tttgcatcat ttgttgtgag aaggagaatc acaggacaga acacagacat
99061 aggtcatata agcctgttgt tccagccacc ctctcaggct ggtgtgttca cRgttctcac
99121 cagactggtg tctgtttaga caaactttgc tgtgggtccc caRtaagaaa aagaaaggat
99181 gaggttcacc ttttatcttg ttttatgtcc tgagagcttg gctttcatga ccactgagaa
99241 cactggtctc tggtctctgc caggtgcagg ggcacaagca ttggcttgtg tcaggcagcc
99301 aggtgaacag ggtgggaacc caagacacaa gtgacaagtg gtgttctctt tgtttgatgc
99361 agctcagggg agtttgtctt aataagagac ggcagtccat
[0378] FollowingisagenomicnucleotidesequenceofaTRPSl region(SEQIDNO:4).
>8:116488301-116587100
1 ccatttcgat agatgatcat tggattaaaa aaattaagag cggaatttta aattatatat
61 attttgtttt tttccagtgt atttagtaca caatagatgt ggtgccaaca aaaaaactac
121 taaattattt ggtcagaaaa taacaggata cataaaaata ttttctgtat tataacttta
181 aattcaaaag gttttgtccc atattgtgta Racaacatta ataacaatat ggaaagaaat
241 aaaaactaag aaaaataaat taaaaagtct taagtctgta tttactaaaa acataagatt
301 acagagtgaa gttctatgac agttcaaaag aaaagaagaa ttaattatat tgaatataat
361 gtttttccaa aactctaaaa tRtaaattgc taaacaaaat aatctagaat atgcttttca 421 agaaatgctt tttgtaaaat ttgtaactat attttggcag taactttgca aaatttatag
481 ctccctttta attccatcta aaatattcaa ttcagcaaag acggaaataa gcatctagat
541 tctaaacact gattcatata catacctctt cactgtcaat gtaatcataa ttgttttgca
601 aattcatcaa cagatgacat ggaaccactt taaaaaaaac agcattaata tatgtcctga
661 ttgtgattgg cagttttaat tacatgaggt ttttatcctg tcagcagttt caacacagtt
721 atccttaggt ttaagttcca ggaatgctct gtatgcatac atacccatac attttgtgcc
781 ctaggagatc aggagaaaca ggctaaagag caattaaagt acagaaaaaa gaaataacat
841 aaaactgggt attttccagg ctcatctcac ctcatttatc tttcagctct tctccctata
901 acttgctgta gattcttggt aaagccattt aacctgttgg gagatgaact agatgacaag
961 tataatctac tcagtagtca aacttattgt ttatgtaggc tttggaaaac taattttaac
1021 atgaatattt taaagatgac atctattatt tttatctgtt agcactgcta tgaaaaatta
1081 tgcaaaactt ggaaattagg caaaaaataa aaaWgtaggt ggcatgttct tttaatatac
1141 actagggact gtttgacagg ctcacaaaag gattatttaa ttacctaagg gaatgtattt
1201 gtgtttgact ttgctattta ctattaatta aagttcttag acttctgtga agtgacatgt
1261 taattttctc cccaaaagaa atgataacct caaaggttaa tgtgccatta ccttattgga
1321 cagtattagc ttcagaaggt cacgttcctc tgattttcct ttaaagcagt ttttccatct
1381 cactattttc cttattaatg agttaaattt ttaacatatg tttactctat atttgtacag
1441 gtgtgatgtt tcaaaccttt tcataactat tcttcgacag ttttggaggt tccacgctta
1501 tgcctcccag aggtgattta cctactagaa atatttaagc aaaggcacac ttttttYtgg
1561 tcccaggagt caatttcaaa caatagaagt tatcaatatt agaactttac cttctgtaag
1621 agttctctat ttctctttct tatcccaact ctatgtttgt gttactattt tctggttcct
1681 agcctacttg tttggttctt cttctggtgt gcacacagca aataagttat tcctagttgg
1741 tgacggcaat gatagtgaat cctactgctg accatttggt aatctctaac tacgattctc
1801 tattatccac tggatgagag gcaacagaga ttgtattttg tagtttcttg tattgattcg
1861 tttgtgcaag Macatatttc atacacagga aaaaaaaaga gaagaaaaat ttcacatttg
1921 aagcagtgag tttttgtgtt tgtttactct tgacccaagg ctgaggttga agaatggaag
1981 ctgaaagctc tctgtgtttt ctgtgtttat ttaatttaga aaggacttca tctctcactR
2041 taactttcag atgttttcct atatccacga tgtgttaata aattatacaa taatatctct
2101 aataatgagt tctgttatga ttgctttgag aagttaataa tcacattcat ctaacgttcc
2161 cagaattcca aaaaaataaa aaacacctgt atttaaaaaa tttaaatgtg ctaatacttt
2221 atttaaaaaa tagcaatatt tgtagaagat acaacctcag agcattcttg aataaggata
2281 taaaccaagt ttatgcaatt aaggtaaata ttcaaactac tatgacattt tttataattt
2341 tggtttgaaa acactatata tctttccctg attttaccaa tattaagtat attccaagat
2401 tacaattatt ttttaaataa aatttggttt aatgtcctca tgaagagacg aattaatcta
2461 aacttaaacg tattataaac gtttactgct aacaacaatg tcgagcactg agaaagacat
2521 aaaactatga attcaaccaa aatcttttta tgtcaacaat aagtatgtgc tgtagtttgt
2581 caggttaaac ataattaagt cgatttaatg gatcgttttt agataactgg tgaatttcta
2641 cgtaagatag ggtattgaca aactaatcac caacctgtta gaattatagc atggctatat
2701 ctttgagtaa ttataatgta agtacttttt cccacaataa taaagtgtaa tatcaatgag
2761 tatgacagta ggagattgtg tgatggcatt tatgagcttt gcatatctgt gaaaatgact
2821 ataaatgata gatatatctc aaatatctac ctcttagctc tctgtgttat aattcatatg
2881 ggtgctccac actttataca agcaatgggg gagaaaagtt caactgtgta actaagttat
2941 ggattatgtt cttcatttgt caagtggaaa ttttttaaaa aagaagtttt caaagtaaag
3001 tgtctggtcg ctttataata tgaaaaggta tggtgaaaga caaaacttaa gtgagtacaa
3061 gttgtgtaag aggtgagagc tgactcagtt tgataacacc agaaaataat gagcctgaaa
3121 ggaaataatg aaatgtgtga aattttggta agttgataga cttattcata aaataatatc
3181 aaagaaaaga aagcatgtaa attatttgcc actacatatt atgttaatgt aaattcgaca
3241 tatttttaat aactgttaaa ataatacatt ggtcttacag catttactct gttctcagca
3301 aagattagta aaaggttatt ttttaccttc tgtaataaat tcagataaca gggatgtggt
3361 ttaccaccat aagtttctat actttgagtt ttttgttgtt gttgttgtct ttgttaagtt
3421 cttgattctg aaagatctac agttcatagc aatcatgtta cttccatgtt gatttaaaaa
3481 tttgcattgt ccttctgatt aacaattagc caagctattg tttctaggca cttttatcct
3541 atttgaatca cacaaatccc ctgtgataaa ccttttgatt ttgcttttcc aagattagat
3601 cctgagttta ggggagaaaa aaaaattgtt ctcagagaat cccaacatat cccaaaatat
3661 actgttaagt gcaagtttta cccttaacag aaatatataa gaattgtaaa ggaccgggaa
3721 aggcaagagg cttaaaaggt ttcagtaatt ttgataaatc agaagggctt aaaaggtttc
3781 aggtagtttc ataaaccaga aggagggatc cagaaaaaat agtgtttgat ctcccttccg
3841 tcaggaatat gcaccatggt aagacttggg gtatgtaatg gctatgcttt tcttttctaa
3901 ggtggttatg aacatgtggg tacatgtgaa agtttagtat ctggaaagca attccattaa
3961 aactatctat ataagagctg cagtgagccg agatcactct actgcactcc agcctgggca
4021 atagagcgag gctctgtctc aaaaataata ataactatcc atataaacca tttggaaagc
4081 tgtctcaaac tctaggcaaa cttgcattta aaagactgct gattaatccc taaattctat
4141 tctgaatatt taaaggtttc tctgtagtcc gaggctttaa tatatcgcaa agccaacctg
4201 aaacaaaaca aaaaggctat ctaaaagagc atgatgataa caatatccgt tgatacatgg
4261 atgagtctcc ggctaaggct tggctgtcaa aaggaagctt tctttccagc ccatcaattg
4321 agtgtcagga ttgttactca aatgaagtga attgtaaaaa attcctggcc aactggaagg 4381 ctgacaaggt tactgccaaa atcaagatat agaaaagaat cttccattct ctttccaaaa
4441 tagttttaag actagtttag ccttcacgaa aaataaaaga atacaatcta tcaggacact
4501 atttcacaac aattttacaa acaaaaactc cagagtccag aaaattgttt tttggtcaag
4561 cttcttaaaa attatccata actgaattta ttcaatcatt tctatgatag tgttattaaa
4621 ataataagat aagtgggtga gaatgtgttt tacttcccca tctagatgct aaatatgtac
4681 attaagggac agatttggct ttaactggtc gctttattgt ctgctcttca gcaataattg
4741 gcatgtgctg atagaccact gtattgtttc ataacataaa acactcacaa ttctgatttt
4801 tgttttcttt gcattttctt cttcttcctt tttttttttt tttttttttt ttggcatctt
4861 gctctgttgc tcaggctgga gtgcaatggt gtgatctcag ctcaccgcaa cctccacatc
4921 ccaggttcaa gcaattctct tgcctcagcc tcctgagtag ctgggattac aggcatgcgc
4981 caccacgccc agctaatttt tgtattttta gtagagacag ggtttcacca tgttggtcag
5041 gctggtctcg aactcctgac ctcatgatct gcccacctca gcctcccaaa gtgctgggat
5101 tacaggtgtg agccaccaca cccagcattt tcttcttatt tctaagagta atatcagaaa
5161 aggcacatgc catttgtatt aatatcaact cttactctca aatataaatc tgtggtaaac
5221 aaacaataga aagttctaaa ataatataaa atggcattaa aatggagctt ctttgaaaaa
5281 aaatctaatg atgtatgaat gcaattaaat taactgtctt caataaacat cttagtatcR
5341 atcaacataa ttttacatag ccaataccag tgatcaaagt tgcttataga tatatgtaac
5401 actataatag tggttattcc aaaatgtata atttcttgaa cttttacaaa gattttagta
5461 tggctgctaa tgaaggagtc attagttcaa acaactgtgt cctttaaaga tttgtttctt
5521 gtcagtacat agaagtacat cttttaaaga taaaaaatgt atttattgtt atYttcacca
5581 agtcttccga aagcacctga tgcattttat ttgaacaaaa aaaactttca attttRccag
5641 aaattaagta tcttatggtc agatcttctg gcatactctg gatttagtga aagcagatca
5701 gttctcccta tgaacatacg aggattgtat agtaaaattt ctcataagat acagaaaacc
5761 tatcagagaa aaatgccact aattcaatgc cctacaaatt gaatttgaat gaaattttaa
5821 ggacttctaa attcagataa agtaagaatt tgtctatagt acagagaagc caggaaagaa
5881 gaagctgtat tctatctcta tcaggctcac tcagcgttcc acatggtgtt gtggaacgtt
5941 cataagtacc atgtatgcca gtgtataaga tatttcatcc taaacaatgg tggtggtggg
6001 acaggcagca gaaaaaagca acaggaatat atttaatatt ttatgggtta atatgtagaa
6061 tggtaataac cattctcaat ctgattattt agcagattcc tgaaaactat agtgYtgatt
6121 ttcattaaca tcttagtaga gtgagagagt ggttagaaac aaagcttctg aatacaaaaa
6181 gataatgtaa cagcaacact tattcttttt atattgtctt cacagctatt tctaaaactc
6241 agatgcaaag cacatattct ataaattgtg ctaccatatt ggacagcaca tactatagaa
6301 aatttccaac atcacaaaaa tttctaagag cactgaaatg tggaggagtt caaagtagtg
6361 gaaatctata caggatttca cttgtttggt ataaccaatg gaaataattt aaaagacaac
6421 aatgatggtg tgttaagcaa atctttcagc taaaatggta gtgataccac tggcagagta
6481 catgctgaaa aatactgttg ggcaataaat tatttgcaaa gattttacac tgtgggaaga
6541 agggcagaac acaatatcaa gataaattag gctaacatga aaacatgtaa aatgattaag
6601 aacgttctgg gtctcgagag cagcctggcc aacacggtga aaccccattt ctactaaaaa
6661 tacaaaaatt agtcaggtgt ggtagtgcgt gcctgtaatc ccagctactc gagagactga
6721 ggcaggagaa tctcttgaac ccgggagctg gaggttgcag tgagctgaca tcacgccact
6781 tacactccag cgtgggtgac agagcaagac tgtctcaaaa aaaagaaagg agataaagaa
6841 tgttctgggt catctactca aactggtaat gctggtaata ctagaatgga ctcaacatgg
6901 gtctagtttt ctaaaacctg aagatgtttg aattagtagg taatatattt taaaaaacag
6961 tattattatt atctttcaac attctaaatc Kgttgtttct ttcttctatt ttccaacaat
7021 ataatacatY tacaatttgg acaaaaacaa aaatgttaga aagggactca attctgaaag
7081 caagagagaa gagagaatgc ctaagtactg taaattttaa aataacaaaa tcacagttaa
7141 aaatcacaaa ttcatgcctt atatagaaat ggcaataagc tatgtgctaa agacaaaaca
7201 ttagttctag tacattagaa aagggatccc cagactgtcc tgccttaaat tatgtgtcaa
7261 agaaccagac tatttgtttc tactagcaat aaattagata gtcacacaag gattaattac
7321 agaattgacc cttgaaacac tgccagtagc ccaattttga acaataactc aaacttggtc
7381 tcctaccaag gaatttttag gaaggagaat ccaaatggtt taatttctag gagagataca
7441 ctgatgtgag gcttcctttc atagattttt ccagctactt acctaccctc taatatatgt
7501 ataaccctgt gtttctttca agggcccagc atcctttggg aacataagac tgtgcaattt
7561 atatacaagt tttccagggg atatcacgtt tcctcataaa ataaaattgt tattactcat
7621 ggcttcaaga atttgataaa gagaaggtgt tgattatata agctgatata aacaaagact
7681 tcagccctca gccttttttt ttcattctga ctccgccttc ttattaattt ttagtagtca
7741 cttaatcttt attctctaag ctctaatctc ccaagaaaat atgtgcctca caaaagaatg
7801 atatcgttta ggtgagtaca ttacctgcta tttttcagat gtgcaaaatt atatttaata
7861 tacaaggtga aatagcttaa ctccaaatgc gtgtgaggct ttgttcagtt ttgaagtcag
7921 aaaaatgaat atttgaagat catactattc ctgacaactt aaccaataat gtattcaatt
7981 tccccaacat ctttcatact gagttcatat attaaaaaaa agttgaaaaa aattcagtgg
8041 caggattaga acaaatagaa taaaagaata gttacaattc tcactttttc actttgtttc
8101 taatgcacta atgggcaatt tataataaaa ctgaaactgt gaggtaaagg tctctttcac
8161 tagccaatta taccctcact tgaaaatggt cctgagaaga aaagtcaact ttaaaaaagt
8221 acagttaata Ratgaagaga catttacctc cccaaatgct caggataatt gagcattaat
8281 aaaaagaagc cattctggta ttacacttca gctaataggg ttgagataaa ctgttccttc 8341 taaataaaaa tcgaacggtc atggttatct attctcaaag tgtcagaatc tgagtaaggt
8401 accccaaatt agaacctttc taacacatta ccaggaaatg aggacgattt tgtttccagc
8461 catcttagat ttcaatggac aggtaaaacc ggaaagatca aagcataatc aattgcttgt
8521 tcatatatcc caaatggaga attataaatt ggcctgctct ccttcttgct gacattcttt
8581 ctgatttcag cattaagaag caatcactgc agaaaaaagt aagtaactta aatagtcacc
8641 ataaaagact gatacaacct ttctgggaaa gtaaaactga aggctgatta tttactaaac
8701 tctataataa tttgcaagct gagcaattta cgtgaaaaaa gttgttacac tcccctttgt
8761 acataactat aagattattg tgtacctatt tatcatagga aatgagttat aataacaact
8821 aatatatact gagcacttaa gagtcacaaa gcatggcatt aatttgagtt atacgctacc
8881 tctcttaatc ctcacagcag cactctgaga gaggttaaat agcactcgca tagacagaaa
8941 atgagagggt caggattaca accaataaag tatgagtcca caccaataag gaagccacat
9001 ttttatttaa atacgatttt aaaagatttc aaacacacct gatctctaat tttcttgtgt
9061 tacattcata tgacacctta aacttcactg accattatta atccagctac tatcctcaga
9121 atggtactag aaatcctagg gtaataaatg ataattgaaa aacaaaattc aaaactaacc
9181 cagtagacag tgttcagatt aatcagtagt ctttaactac taatcacttt attataaata
9241 gtctctagat gacgtataaa atctcatcta gaggcatata aagatatata tatgtatata
9301 tagtcattca actgtagaga tggtataaca tgcagttata aaggacaaat actaagttcc
9361 ctaatttttt atgattcttt taaaaactat ttcaaaatag aatgtgaggt caactagaat
9421 agaatcacaa atcagagtag ctaaacatta tgaggacaaa caaggtacac aatggttacc
9481 gttaagaacc tcaccaagag aatgaaatta agaacagtat cactggcatc atccagttaa
9541 acaattaagg aaccaatgct atgagctagg aaggagacga ttaaaaacta agctgaggca
9601 gtgacacagg gaatataaaa aggaaatgta catgaaaaac tggcaaaaaa ttggcatttt
9661 ggctgattgg ctatcaggta agaatgagaa ggaataaaat acagctggag attttttttg
9721 caggagaggt acagaaatgg tgtacatgcc agtgagcaga actgtggagg tcaggtgagc
9781 aaatatgtaa aatctaagtg tagaccttca aaaacttatg caaggttttg ctgtgtcttt
9841 gaagaagccg atctgagtca aattttaaaa taacaattgt atatgtaaga tgtagattga
9901 aaattacacg atatatattt tcattattaa tattgcttcc atgtttttat gctaattaaa
9961 aggcatgatc cggaaatcac tgaaaaagat tactcttaag aattgatttt acattaacaa
10021 aaaaatgtgc cttcactgga caggttcttt caacatctta gtgcagtaaa catctatgac
10081 tacctctcct gaagaagaga aaggtttacc ctacagaaaa ttccacaccc attttgcacc
10141 ccattcatat gcatgagact ggagtcatct gcagcatgtt tccacagcat aaaatctgta
10201 ttatattaca gaaagtgtcc agtgtactta tttattttgt gccaacaatg cccactatat
10261 tgagcagaac tatagaaaat gagagggcaa caccaggtag ttacttgata actctagttt
10321 tcagcacctg gtaaaccagc atgtagaaag catagttcct caacattcag aggatgctaa
10381 tcacacaaaa tatctaacag taactggcag ggaaatataa aggtgctttg tgtataggag
10441 atctttacaa aattaaaatg gcagagatgc attaaatgag aaaatccatc agaaagaaag
10501 gggtaaagac agggtaacca aaagctgagc ctgaatatcg ggttcctata ttttcctccc
10561 aatctgaaga acttaataat ttttttaaaa aagttcatgt tattagtact gtaggcattc
10621 taataaatct cataaactga ccgtgttgtg tcattattct ttgaaggcct gacttttaat
10681 gtcatgataa tattccataa tattccagca taatgtacaa acctttattt tacctgactc
10741 tgttttcaga tattctataa atatcatgac ataaattagt aaatattgac ccccacattc
10801 agcactgttc tgagaaattt agagacaata agagtattgg tgaaagacaa gacaaagtcc
10861 agaaatggta aaatgtgaag tcgtcttggg agaaaatagg ccagtaacct agggatctgg
10921 tatagaaaga gcacttaatc agtatgagga aacaagttag gtgtatagtc ctgggtaagg
10981 caagacaaaa ataatccacc ctgattgaac ttagatacgt gtgcaaacca gtgtgaaaac
11041 atggagtaac actaaggtaa atgcagtaat tacaggagaa acataaccca tttgtcaggg
11101 ttgtcaattg cagtaaaaaa tatgttaagt gatttttccg tatgtttatt gtggcactat
11161 tcacaacagc aaagacttgg aaccaaccca aatgcccatc aatgacagac tggattaaga
11221 aaatgtggca catatacacc atggaatatt atgcagccat aaaaaatgat gagttcatgt
11281 cctttgtagg gacatggatg aagctggaaa ccatcattct cagcaaacta tctcaaggac
11341 agaaaaccaa acactgcatg ttctcactca taggtaggaa ttgaacaatg agaacacctg
11401 gacacaggaa ggggaacatc acacaccagg gcctgtcatg gggtggggga aggggggagg
11461 gatagcatta ggagatatac ctaatgtaaa tgacgagtta atgggtgcag cacaccaaca
11521 tggcacatgt atacatatgt aacaaacctg cacattgtat acatgtaccc tagaacttaa
11581 agtataataa taaaatttaa aaaaagagta tgttaagtga ttttcatgtc agaacaaaga
11641 ggtctctaaa gaacttttga tttgttaatt ttatgaatta acaagataac tgcaaaaacg
11701 aatgatatag gtgcctgaga aattttgaga aggaaacatc aggcagactg aagtaataga
11761 gaaaggcttc ctgacaatat taaaccggct agttctgaac tttctattaa attaaatagt
11821 caaagtattc ttgtaatcaa aaatgtttgc aaatcgggtc actagacaga ctagcaaatt
11881 caagatactt gttgattact gttgaatcca ttaactacgg ccctaacctg gcaggaatct
11941 ttcagctcta ggccatgagc tgaaggaagg gaaaaagctt cagaggaaaa tgtaaagaaa
12001 ataggggata atttaacata cttttgtgca gaaataaata gattggtaga aaggaagact
12061 gaaatcaaag gctagtgtgg aagtcagaag gacggatgaa agggattaaa gaagacaggt
12121 taggaaagaa ggtggagtac cctttccctg ttaggaatgg gaggaagctg gggatgcctt
12181 tgtggtaaaa taaaatatta cttacaaaga gtaagagttt agaatacata gctgagaagg
12241 gttaaaaaaa acaaagtgaa gagccttgtt tgggaggaat aacatgggca gtcaaataaa 12301 gaccagtaaa aggatcgctg agcacaagtg gaggcataac agggaatcca gagcatgaag 12361 acgtctgatg gaggcgggtg cttatttcat aaaggctgaa gaaatggaga aggtacaaga 12421 aagaatggga ggatgtggtc acagatcggg gtttctgagt tgctggttgt atggggctgt 12481 tgccatgagg aatgacgaaa tctgagggac tgcatgcttg tgaatatctg aagaaaaagg 12541 agtcgaaggg ctactgacac tcacgatgtt gagatattca taaagagaga tattgggaga 12601 gtcaccaaac atacaaaacg atatacagat tagataatca gaccttggaa aatacatatt 12661 tgcaccagga tttcaaaata tctataattg gtccaatact tctgaaataa gtctacctaa 12721 tctaagcaag aaatggaatg aaatcagagc gcattttttt ttttttacca tacatggtaa 12781 atggcatggt aaatagtaca gcaaatattt gactgatgaa aactgtaagt cagagaatag 12841 atgaagaatc tataacccaa agacttttca ggcatattgc agtaaattaa aatctacaaa 12901 tactggctga gggattattt caatcaggca ctaagcttgg tgtctggaat atgtggatag 12961 gacatagtcc ccatcttgga agctcagtgt ctagaagaga aataaatctg taaacaacta 13021 attataatat ggtgccattt tagaaagatc agtaaaatat acaggacaga gctactccct 13081 aatagtaggc ccaatgagag aagacttccc tttggacttg aagggttgag ctgagttctg 13141 aagcatgaat agaatttata ttgagaccaa tatatttata ttgagacagg cactgtacgt 13201 taattatctt ggtagtcctg gacacaaaaa gtcctcaaaa tctctgagta gtaagtgaat 13261 aaatctgact ggtctgcaaa ccatgagaga ggaatatcag gagatgaggc tgcatcagaa 13321 gagtagtgaa taaatgaagg gctttgggtg ccacgaggat gataagaagg cagagaaggg 13381 ttttattcaa gagccaaaga ggaacattat cagatttcat aaaggttact ctgttggcag 13441 agtagaggat agattagaga caacaaccca caggaaagaa gagaagttca gcagctattg 13501 tgagagttta gaaaagagaa cacggtctaa accaaaggag tgaaacagaa gataaattag 13561 tgattgacag gatatgagtc ccaggtgttc tgcactttaa cttctatagg atctaaaatt 13621 tcatactgga gcaacactga atgctggaat atgagagagt aaattctagc ttgagcaaac 13681 ttttaatggc tcaaaaacag aagattcagt cttaactgtg aggttatccg ctaggttgtt 13741 caccacactc tttgagaaaa ccYcccaaat actctcccac caccctctgg tgaatcaagςf 13801 tcagtaggaa aactagttcc aatatttcta atgttgtcaa ttacggtaaa tcctgtctac 13861 aatgccacac agggagtacc tatatgaact ttctgattct gaacaagacc aaacattaag 13921 gggcccgctt cccacatgta atctgttttt tttttttttt ttttttttYc tgaaaaaaat 13981 catgagtgag ttaaatttaa atatttcaaa catgcaaact ctcaagaggg taccatgatt 14041 acagggatta aataacataa aggtttccta caaactctac acctgctttt gccccttaaa 14101 tatcaaatgt agttacattc tggtgcaaac ttaaaagtaa ttttcaaatt ctgtctgaac 14161 atggtgtctt agtctgcttg ggctgccaga acaaaatacc atagactggg tggcttaaac 14221 aacagaaata aattttctca cagttctgaa ggctgggagt ccaggattaa ggttttgcag 14281 ggtttggttt ctagtgaggg ctttcttcct ggcttgcaga tggccatctt ctctccatag 14341 gctaacatgg cctctacttt gtgtgtggga gagtcagaga tagagcaagc cctcttgtgt 14401 ctcttcatat aagcctacta atccccatca gaccaggtcc cttatgatct taaaccctaa 14461 ttacatctta aaagccccat cccgaaacac catcagactg gagattgaag ctctaacccc 14521 aataccatca cactggagat tgaagctcta accaccacca ctggggattg aagatctaac 14581 cccgatacca tcacactgga ggctgaagct ctaaccctga taccatcaca ctggaggctg 14641 aagctctaac cctgatacca tcacactgga gattgaagat ctaaccctaa taccatcaca 14701 ctggagattg aagctctaac tgcagtacca tcacactgga gattgaagct ctaaccccga 14761 taccatcaca ctggagattg aagctctaac cccgatacca tcacactgga gattgaagct 14821 ctaaccgcag taccatcaca ctggagattg aagctctaac cccgatacca tcacactgga 14881 gattgaagct ctaaccccga taccatcaca ctggagattg aagctctaac cgcagtacca 14941 tcacactgga gattgaagct ctaaccccga taccatcaca ctggaggttg aagctctaac 15001 ctcaatacca tcatactgga ggttgaagct ctaacccccg gtgtgatggt atttggggag 15061 caacattcag tcataacaca tggcaaagga ctgctgaaaa agaaaacatg tttcatgctt 15121 tgtgtcatgt aaagacaaaa gagacaactc cccaaaactc attctctttc tcgctttttt 15181 tcctttttga agatcaaggt atgttaagca tatcaacaac agtgatattc agagagtggt 15241 aataatatat gatgctaaac tgttttggaa tgagattcaa gcagcagcag aatgtggaga 15301 aagaaactac attacttaat cctttccttt tcaactacca gtttactaat tatttgggta 15361 aaatataaat actagtttta aagttagaaa tacactagga acttactcta ctgccttcta 15421 taaaatgaat gctttaggtt ggagaaggtg aagaaaaagt ccttttgaaa agatatctaa 15481 agtaaaacac ttggcatata aatatatata gcatatatat agcatatata taagcatata 15541 tatatagcat atatatagca tatgtatagc atatatataa gcatatatat agcatatata 15601 tcgcatatgt atagcatata tatcgcatat gtatagcata tatatagcat atatatagca 15661 tatgtatagc atatgtattg catatatagc atatgtatag catatatata gcatatgtat 15721 agcatatata gcatatatat atagcatata •tagcatatat agcatatata tagcatatat 15781 atagcatata tagcatatat atagcatata tatagcatat atagcatata tagagcatat 15841 atagcgcata tatatagcat atacatagca catatatata gcatatatat agcacatata 15901 tagcgcatat atatagcata tatagtgcat atatatagtg catatatata gcgtatatag 15961 tgcatatata gcatatacat atcacacata tatagcatat atatagcata tatatagcgc 16021 atatatatag cgcatatata gcgcatatat atagcgcata tatatatata tataattagt 16081 atttcacagg ctgggtgcgg tggctcaccc ctgtaatccc agcactttgg gaggccgagg 16141 tgggcagatc acgaggtcag gagatcaaga ccatcctggc taacacggtg aaaccccatc 16201 tctactaaaa atacaaaaaa aattacatat atatgctaag ttttttcata tatatataca 16261 tgtatatgtg tatatatata tgagactgtc atatatatat atatgagagt caagcctttt
16321 gattcaccta ctttggatac cagttaaagt tagtgcttca tctctcattg ttattaataa
16381 aactgcagag cattataata tgaataatat ttcaggctca aaagtctcac agttcatttt
16441 atgtgtgata attacattgc ccactactac aaaaataaat acactcaaga aagctaccat
16501 tttcacatca taacataatt taagactgaa ttgtacaata ataaaattag atgattatta
16561 ctcaaaatat aaaattaatg agccaatcaa tgtaaagcac taaggtacgt gttaccaaaa
16621 ctattcaact gtatacaaat acgtaataca gacaaagatg taattttaca taatgtaatt
16681 catttaatcc taaacttgca agaaatagtt ttaaaattct agcttaccat aaaaatagta
16741 tttcacaggc tgggtgcggt ggctcacccc tgtaatccca gcactttggg aggccgaggc
16801 gggcggatca ggaggtcagg agattgagat catcctggct aacatggtga aaccccatcg
16861 ctactaaaaa tacaaaaaaa aattagccag gcgtggtggc gggcgcctgt agtcccagct
16921 actcaggagc ctgaggcagg agaatggcgt gaacccggga ggcggagctt gcagtgagcg
16981 gagattgcgc cactgcactg cagcctgggc gacagaggga gactccgtct caaaaaaaaa
17041 aaaaaaaaaa tgtatttcac agcattgtaa aatgccattt acatactttt aaaacaactc
17101 ctcatttttg ccattactat tttgtgagtt cacattttac tcttatgtaa ttaatataaa
17161 aggtaccata aatccagttg gcaaatttgc caacctgttc actatcatca tcccagttga
17221 tactttactc aattaggccc tgcattttat cctaatgttt aggacttcag tgagtactta
17281 ctaaatatag ctgaagtttt ttttttgttt tttttttccc tcttagtggt gagaaataga
17341 tgcattttta atgttttaat aatttaaaaa tattaacata aatttgaagg tcagaatttt
17401 acatataggg ggttcttcct ttatattcca tttaaataaa gttataaatt gccttaaRgg
17461 aaaattgaat ttgttttgac acattagcaa aaatttcctt atcacatcac aaatcatgaa
17521 taaaatgctg ttacagagca acagagggag ctcacacata tttgattctc tccctgtctc
17581 ttcactctgg ttagaaaaaa tgaagtttgc tttaaatttt ttattagatg attcccccac
17641 cctgccaaaa taaatccaaa tatatttttt aaatcaacaa ggaagttaac tacaagtata
17701 ttcttccact ctaaatctta gatgttgtgt tgtttttctt tttataaaat aatttatcta
17761 agaaatgctt ctggaaaacg ttcatagtta tgattcattc tgtttactat attgtgttaa
17821 ataaaatgtg ttctcaaaat tatacatgta aatgtttgag aatgagaatc aaatcttaaa
17881 cacaagcagc caaaatttca aaatcaaccc ttacctacag aggactataa agaaatctca
17941 ttaacaacca cagaaactta ataaagctaa gaaaatcaca tataacaagc catcagagaa
18001 tccagacaac ttctgccaca aaacccataa gtcgatgcct otgcaaaatt tatttaaata
18061 ccatgtttac gtattataat aacttgtctt ctgataatgt tttgggggac ccaaattagt
18121 ctcctataaa aaaaagacat gtcctgagac tccaaaataa atttttaact taggagaaaa
18181 tacatatgtt aagatactaa aatataatcc aggaaattca acggtttaaa aattgtattt
18241 attatttttc aaaaacaaaa aatccaggct ttgcaggaac ctcaatgtaa tacgtaagtg
18301 agttggactt cgagcaactt aaaaactgga caattatcct tgctctaatt tgctcccggt
18361 ttaatcttag tcacattgta cagagctaac acttatcctc cagtgttcta ctaattgctt
18421 ggtgctatgt tgactactgg gaatgaacca caagatacat cactgtaaac tgcagggagt
18481 gcttcagtga tcatgaggtt aaaatctccc catcagcaga aagagaattc tttacttaac
18541 ttagtggtac aaattatttc caggtgctga taattatcac ttgtggtgtt tgatattccg
18601 gactttgcat ctaaatttat acttgacaag ttaaactttt catcgtgtgc attttcccgt
18661 aatctctcaa cagccaaaga caaggataga cggattagtg atgggtgaaa ccaattcaac
18721 ccaaaatggc aacacttaat tacaagtctt gttgtttata attaactgtg taaatcacta
18781 acttgaaagt ttcttttcaa agtatttaaa accaactctt ttcccagggg gatttaatac
18841 tgtatattat tgcatagtta agtattattt tgtttgctct atgaacaaga tgtgaaaatg
18901 gcacatttta ccgactatct tgctaScaaa tttggaaaat gttcatgaat tgcattacta
18961 gcaccatttg gtgcttctca gtatttttgg acattttttg ccttatatat gaaagccaat
19021 ggaaagacaa tccaactacc caaatgacta tgagtaattt ctgagagtgg agagtgaggg
19081 tcaaattctc aatttcagaa aataaaaaaa aaaaatgagc ttccaaatgt atcttctgag
19141 atgcattcat ttttctgtga ccaatattta gaataaaaaa ttactgagaa catactctgt
19201 gctagatacc atatgttttc taatattcta atttagtatt ttctattgta atattaaaaa
19261 atactgagag acaagaagac aactcctatt tcactgacaa ttaatctgag attcagaaaa
19321 tgtaaataac ttgttaatga ctactgagcc agtaagaggt tatagatcta tagtttgaac
19381 tcagttcttc taaatctaaa gtcgtacttt ttaaaacaac ctctccaaca taatatcaaa
19441 aaagtaaagt attctcttac cttgcatatS tacatcaaag aaaaccataa ggaaaagaaa
19501 ctacagatgt gcacgcagta agtccaagat gcttaaaaat gtcacatcat gttaaacctg
19561 agtcaaccta acagtaaggc tgtaaaaaca actctacttc ctgatttgtt actgcattca
19621 gctaatgtat tgagggccta taatggctca gatcctagaa atataaggaa taagaagcaa
19681 agaacccttc cctaggctag tgcaggagac ctatcagtaa agacatgact gtaacacagc
19741 atgataggtt cactggtata ggtagcaaca agggtttcaa agaaaggtta gcggttccag
19801 ataaatttca ttttaatttc caatgttatt ttaaaaaatt tctgggctct aactcaatct
19861 atttagcttc agctatctaa tttttgcacc atttctaaat attttatgtc tagtcccatc
19921 ctctacccag ttcctgattt tattatttat tattcaaaaa gaacgaaatg aaaattatgt
19981 agttgaaaag taaagcaatc gaatttgagt tggtagatgc aagaatcagc aaacctgaag
20041 atgggtcaat tgagataaag aaatttggcc aggcatggtg gctcatgcct gtaattccaa
20101 cactgggagg ctgagaaggg aagactgctt gagcccatgg ctttgagacc agcctggaca
20161 acatggcaaa atcccatctc tacaaaaatt aaaataaaaa taaataatcc cccaaaatta 20221 gccaggcatg gtggcacaca cctgtagtct cagctactcg ggaggccgag atagtaggat
20281 tgcttgagcc cgggaggcag aggttgcaat gagctgagat cctgccactg ctccagcctg
20341 ggacatagag gctggaatgc agcctccgac tcaggaatta tcctgatttt gctggataat
20401 atctgccgtc tacatgatcc atcatcgaga atctgaaagc ctgctcctaa gaatcatgtt
20461 tctctcattc ttcaaagtct ttgcagattg tttttctctt gaatgagagt tgcatatgtt
20521 tggcaacaga actttcaaac ttcacacacc acctcttgac cgcaatgtct cttctatccc
20581 acattctttt ctcattcctt agaatttaga attcataatc tgattttatt tctcttgagt
20641 tataaattcc atcatggaag aaagcttaac atgctttagt acaaaataga aaaacagcac
20701 aatggacaca tttaacagcg atatttgaga attcacaaat gttatagtga aatcagaaag
20761 ctagtgggaa agtataatta ataagaaaat gtagtaagac attggtaaat attaaggaat
20821 atattaaaga tcagcaatat tgtaacagaa aaactcaaaa caagcgatgg atgactggtg
20881 gttggttctg tgtgcctcct ataaataaac cacaataaaa cgaaatgagt ccagcaacaa
20941 agaatttagt tagaactttc cattctcaga aaaataaaaa ggattcttga agaggtgaat
21001 aaaaagatct tgagaatagc atttcttgat cttccattac tgcagaattc atcttatcag
21061 ttgactgatc agtagtagtc agttaactga aaaagtcagt taaagtgggg aatcataaaa
21121 aaaaatgtaa tcaccattta aattactaat tatattattt aaattaatta ttttatttaa
21181 tcaggaatta tatgtcaaaa cacaaaaaat acatactttg gctaaacttt gaattacata
21241 ccagttgtct tttttacata aacttgtgca cataatagat cctctaaaat ttcttcattg
21301 catgcctgta tcaaaacaac ttacgcaccc cataaatata tatctaatat atacccatga
21361 aaattaaaaa gaaaataaaa ttttcaaatg cttatgatct tttccccaat ctgctacaat
21421 tatctcaccc acacttaatc tgacacctca agtgatacat ttttattaaa tctttctttc
21481 tgaagcattc atttttgtgt cactgaagaa cttccttttc attctcagat tttatacact
21541 tatataatat ttaatttatt tacataagta tattaataag ttcacatacc ataacaggtt
21601 tggaacaatc cccaacaact cttggtgaac gtacaggtca actaatagta gcagaagcga
21661 atgtatgtta cattagagaa caaagcctac tatctgaagg cactcagcat gctgtggaca
21721 tcagatggtg cattttacca agaaggtcca gagagaactg gttacatagg gatggcttag
21781 gagggtttct tactatactt ttttgatatc catctttctt tttcaccaca gtgcaaaatt
21841 ttcaactcta ccctccactc aatacacatt aatgctttca ccacgaacga taaagaacag
21901 gaaactatag ctattcttta ttctagtgta accaattttc actggttatg ccaaggcaat
21961 agaacacttt cacctttaaa aagtaacctc tctttgtaga catacttcca aacttatatc
22021 cataatttac ttgtgtttta aaatgaaaac tcaataatta aaaattaggt tcagaggtca
22081 agcattccac ttgaaatcaa ttgtttttca gctctgacag ttacatagcc tgccttatat
22141 ccaaaaacta atctttatta acagcattca atctcaggtt cctcatttgt aaaacgaaag
22201 gccaaactaa gaagctactc atccttaata tagtttatgt ttctgatatg gatgtatttt
22261 aaaggaccac attccacttt aagatatact tgacacattt cagccaaaat tcttgaccgt
22321 aatacaatat ttaagtgaaa tgtcagacat aaactgtgcc agaaccctta tatccacaca
22381 cagcaggacc caaaatgagt atcactatac tggattacac caataagaga agtcagaggt
22441 cagagaagcc acacagagcc ctgcaatagt caacaatgca acttaatgtc ctttccatgt
22501 ccagaacaat ttgccataga acttctgcaa aagagattaa tggtgatagt agcagcaagt
22561 ggaagagaaa tgcaagatat ggaagcctag ccaatattcc atttgtaatt tgtcacatca
22621 cagcacaata agactactgc aatttactat ttgcagtgtt ggcaatttac aattcaaatg
22681 agcgccttga tttttcaaag aatggactgt gtacacatca gtcagcatga cacaaaaatg
22741 cccggtcagg ttcattttaa atgcacgttt tatttggtac tgttaactaa tcactatcat
22801 tacttttact gtttacggat gcgatattta ttaggcaaaa tgtaatttgg aatcctttct
22861 tgcacccaaa cctaattcat ttcctgactt tccgaaatat ttatatcctg attagacaca
22921 ttagatcttt aaagctatta tactaagatg atttatttat ttctgggggc agtgttaaaa
22981 aaactactct ctaaaaggaa gactgaaagg aaaaacgcca gagtcctttc cagaacttag
23041 aaaaaaaaag aaataaagac ggggagatgg ggagaaaaag ggagaaggtc taatttaggg
23101 ctgtggaaaa ggatttaggc gccggccact cccaagtttg caccctgctt ttagactctg
23161 actiicctcct atggcccatg gcaagactct taacttactt gaatcaattt ccacatctgt
23221 aaaatgggga tgatgatact cacctcacag acatgttgtg aggattaatt agtgcctgtg
23281 aaatgctttg aagatgaaac gggttatata aatgctaact attattatta tgagagaact
23341 gcccaatgct ataattacag ttttaagtcc cacaatatga cctactctga aataatcata
23401 gttccatgag ggaaaagcac aagcttaagc tttctccttt caatttattg aacaaataca
23461 gggagaagac tcacattttc tcttttatac ttgcaaaaag cagagaatct atactctcct
23521 taaccttgac ataaacgatt tcagcaagtc attatggcag ttttgaagca ggttggtgtc
23581 ctaagggatg tggaggccca aaggacagac gggtaaaaaa aaaagactga gaatattttc
23641 accaccactt gaaacacaaa gggaaaataa gtaagtgggc tcgctgttgt tatcctcact
23701 atcactgtta tagggaagca ggcatggtct tatggaaaag acacagcttc caggacctca
23761 gtgtaccaca tagaggtgac aaggattaaa ctaaattgtg gctatgtcag ctctgttgct
23821 attgctattg ggttatgtat tacacatcca cctctaatat ggagttttat ctattgtctc
23881 tttcaagatt agcaatgaca taaaaattgc caattagctt tcaaaagatc caataatttt
23941 ttttaaattt tttttgccat ctgtgatttt tcttctgaaa gaaccaagtg tttatttttt
24001 gttattaggc ctttggttct aagatatgca agtgagcatt taaaaaatat atatctatat
24061 accaagataa aaagttttaa aaattgaggc taactcttta gcaaattgaa aaatgatgtc
24121 ctYatttttt cttcttcata caagattaac tttagcgatt tgtttaagcc aagaatcttt 24181 cttcttttta tgtaccaKga gaaggtgaaa aaaatgaacc aaagccaaca caatactgta
24241 agtcaagtaa gaatctaact taaattagcc tggattttaa aatgacccct tcaccagttt
24301 atagatcaat tacctatGtt atacaattct ttgatttgtc ctgacaaaaa taatttaaga
24361 agtgatttct ctgaaatttg ctctacaaca cgttcgatgc tcatgtctgt ttaacataat
24421 attgacagtt cccttaataa taaaacataa aaatgcaaat atcctaaaca ttaagacatg
24481 gaatttaaaa actgttactt gtaattcatc aagacaaaag tgaacaagaa aatgtattta
24541 gacataaaat aaaatcctcc tatcaagaaa tgtaaagaac Rtttcaggct aaaaagagaa
24601 taatttcaaa cctgtgaagt ggcatttttg tgaacacaac aatggaaagg aagcagaaga
24661 aacaagcggc taagaggaag gatctagtat gaaatctcaa attgcagccc atcactaagt
24721 agcctaattt aactcccagc cagccacttg ccttcttcag gcctcatttg ccttatcagg
24781 gaaatgggag ttatagtatc tatctcatag cattactatg aggatttagt aagttaatgt
24841 ataaaaatat tttacttcct gaccctgagg aatgtcatga atgtgaccta taaaatgctg
24901 gattggaggc aggtggaagg gccaggaagc cactgaaatc taagtcctgt acctccctgg
24961 catctgctga ggatgttgct agagctatac ctaacaccac agtgtgctaa gcaattcttc
25021 caactacacc taaaaatact aattaaaaag ctgtatttta ttgattcact cactaaaatt
25081 attagacatc cagcagccta agaaaaccca tttcatgtac aatatttata ataacttaat
25141 gttatttcta tcaacaagga ttcaaacaca accatttttg cctgatatgg aggcacttac
25201 gtccaccaat gctgtaacat ttgcattcgt taacaccctt tcattaattt attaaatcat
25261 tctccagtgt aacttctgta gaattcccag tttttgcttt tgtgaaattc .tgtagttgat
25321 gaacctcaga ttttacaagt aattgaactt aactacagga gaaggaggag aagaaggtgg
25381 agggaaagga caagaaaaaa agcaagatat aacttttttt ggttcccctc ttttaatatt
25441 ttttctaaaa ttcatactaa taaatacaat catttaaaaa tgcaggtatc taaaattaca
25501 tataaactgg tccttcgagt aagtcagaga atgctatttg ctcattgtta actgtatttt
25561 tagtatcttc caaacaaaat tctctttatc aaaattatca tttgcagctt ttctaggtag
25621 tttccaaagt ggatgcacgc ttatggttgg aaaggatcct tcttgacaaa gctttcacac
25681 tcagaaacta ctatcaaatg cagtcaagca caSgaagaaa gaatacactg atgaccgagt
25741 atgctgaaat aaagaaacat aaggtgctgc tgtctgaatt cacactggag tttcttccac
25801 tggtgtcagt gtggtaacct atctaattaa actgcacgaa ttatactttt ccttcctcta
25861 ttcagtagca tttttcactg gatggtggaa gacgtcaagt ttaatagtta actctgtgtt
25921 ttcttaaata ttctctctaa ttcctatcta gataattcat atcccttctt cactatgcct
25981 aagagccttg gacattattg gttgctacaa cttaacttaa agagcagaaa gtcattaaaa
26041 tagcacaagg cattcaatgg tgtcaattat tctacccttg aaaccatctg aaattaaggc
26101 caaactaaaa acaaataaat agacctatac tatttactgt acttacttta aaaaaatatt
26161 aaaattaggc agaccatttc ttcattcata ttttagataa agatccaatt cagatttatg
26221 aaaaattcca atacaactaa tgatctcatc agtcaaattc atactatctt ttagttatac
26281 ttgatgaatc aatttttcaa cattattttt aggacaaatg gcttcattgt tttatttcaa
26341 gaacagattc aaagaagtga aaaggaatag aaagttatct gagtgaaatg cacactttgc
26401 aacttatttc accaatttta attcttttat ctaagatatc acagagtaca ataaaacttg
26461 cagataacct aaaacagttt atgatctcaa aaaagtatat aacaatggaa ttacagtgtc
26521 tttaattatt atcatccctc acctaaagaa aaagaatatc ttttcaaatt gtccacattg
26581 tgaacttaag ctagcattat ttatatgtaa gaacatattt agaaaatgtc aatggtgggc
26641 agcaagcaac tttcaaaata aataacatgc aacaaattga aatgaatata tatatttata
26701 taatatgtaa aaattaaatt cttgcattta attaaataca cacatatgta tacactatat
26761 acatatatat ttaaaaagca tttgcaatag ttttaacaag attcccaatg ctagctaaaa
26821 aatgctaaat aaacaaaaaa tataaaaaca tcgtactaat aggtgacttt ctctaacaat
26881 caaatctata ttctaataca cttaagtgat acctgggata ctatttgttt ttaatttcaa
26941 agatataaca gtgatagcat aataatattt gcttttgtaa gtaactctga atttggcatg
27001 ttcttatcct tagtgtttca agaaataata tacatccaat ggataaagta ggaaaaaata
27061 taaatgtaac tctctggcat gtaaaatgtg acacacacac acacacacac acacacacac
27121 agagccacgg aggttttagt gtttaactaa tgtaacattt ccataaatta tttccataaa
27181 ttacttttta aaaatctttt caaaatatct actattataa gtacctaata taagcagaca
27241 tctgtaaact tgggcaaaac agtttacata actcttcatt actgtgagct gaataagcaa
27301 ctttagttaa aatttgctta ctttttaaca cagagcagag gttccttcga gatccaggtt
27361 ggtaaaacag aagcacagat gtcacaagta cggacaagag gcaattatta aataattcaa
27421 agaaaaacgt cccaatccta cgttaactgt tcccacaaag tccagttcaa atgtttaaat
27481 aataaaactg ccagtgtagt tgtcaactac atttgaagtt catcacagac tttgtaattt
27541 cctagaaatt aagtttttaa tctagcatcc aaattacggg atgtttactt aattttactt
27601 tttaattata ttaagaaata tatcaaatgg tataattcat gcctgataaa atcataaaaa
27661 tgccatatca aatagcaaat gattaaaaga tgcttttatt acccatactt tgttaaRctg
27721 actttctaag catcacttgc ttttttaaat caggaaaggg aacaaagaat gactcaattc
27781 tctctctctg tctctacctc tcgccctccc cttccctttc tccctctctc tcttcccttc
27841 tatagccctc tctccctctg cctctccgtc tttttaactc tgtgctaagc tgaaataatc
27901 tgatgagtct ttagctttag ctgggccagt gaagcatggg gtttaagctt gtggctgtat
27961 ggtagtgacc tgagtgtgaa tacctgcccc tagagctagg attttaagca agttatttga
28021 ttctctggtc attaaacgca tcacacacag gttagtgttt caaatagaag gcagtcaccg
28081 taattagctt cagggcaatc agaacttctt tcttaggctt ctctgttttc tcccaacctg 28141 cctccctcta gagagactct tcaccatctt gccacRgtcc ctagtccctt gagctaccct
28201 catcccggga aaggcttcat tcctttcact cccgaagagt agctgccttt atcttagcca
28261 tctctccacc attccttcta cgctgcactt atttcggttt tccttttgtg tcctgttgtc
28321 tactttgaac agtgaattaa ttacctgaga actaaactgt gctgggttgt aaacactaga
28381 aataaattaa cttgtggcta ctctaaggat cacttctatt ttaagagaaa acttctacgc
28441 ataataatcc taaacctggc caggcatagt ggctcacatc agtaatccca gcactttgag
28501 agctgaagca ggagaactgc ttggggtcag gagttcaaga ccagcctgag caacgtagtg
28561 aaacaccatc tctacataaa aaaaaaaaaa aataagtctg gtgtggttgg cgcatgcctc
28621 taagcccagc tactcaggag gctaaggcag gaggatcact gaaactcagg aggttgaggc
28681 tgcagtgagc catgattgca ccagtgtatt ccagcctggg tgacacagca agattcaaaa
28741 aatacatata tataaataat cctaaaccta aagaaatcaa gttttaattg tgggcatttt
28801 agaaaccaaa tcaacaacat tcattttttt gtttttgttt tttgggtttt gtttgtttgt
28861 ttttgagaca gagtctcact ctgtcgccca ggctggagtg tagtggtgca atcccagctc
28921 accgcaacct ctgcctccca ggttcaagcg attctcctgc ctcagcctcc tgagtagctg
28981 ggattacagg tgccccgccc gccaccacgc cctgctgatc gcgaactcct gattctgccc
29041 acctcagcct cccaaagtgc tggaattaca ggcatgagcc actgtgcctg gccaacaaca
29101 ttcatttgga ggaaacctga ggatttggga acactgcaag ccaagatata aagagagaaa
29161 gaagccagcc agaaccacat taaaaccttt aacattaaat tcttatattt ctaataggca
29221 tattactctc ctttgagtct ataattaaRg tttcaggtaa ataagactac attcaaattc
29281 ataaaagaat tagtaaagga tgaaaaatat tctcagtata atctgtgctg tgtcttattc
29341 agaatgcctg tcttaatcac ataggcttct gattgctaac aattaaaatc ctgatctcct
29401 ttttctctcc ctaatagatg aaagcctttg tgaagaagat gtaaaattca ccctgtcata
29461 ttactgacaa gtggcactga gctggtaaag tagtgtatta atactgatta cactgattaa
29521 tagtaccgta aaaattggtt caatggtgct ttaacgtttt ttaaagaaac agatgagaga
29581 cattctgggc agaatatata atagctttac atcattctat cacaaagaaa aatctttgaa
29641 atgtatgaat taatgtgaaa gccgaactgt tcctactgta tatcaataaa ttcattaaaa
29701 aattgaaatg tctaaacatc tctccctaac cccctgctat caggaacctt taagaaaaga
29761 atgctcaagg cttatgacag atcctgctaa attcaatcac tgtcaatctc tttcatgcaa
29821 atgaatatgt attcagcatt atataattgt aacaagagtc aatatcaatt atgtttccaa
29881 tatgacaggt cctaggaaaa catcatattg tataagatga taacatcaat accataaatt
29941 cttctaagca aaagttataa gttttaatac atcaattggt aagaatgttc tcatatatag
30001 tccattgtac tccaatatca aagaYggacg attagtagaa aagggattcc cctgtcccct
30061 tttgacagct gaaaRggcta tctatattcc ttgagttgtg tagttcgccc aaatgtttgc
30121 agctactcag acagacttag gaaccttggt caagtagctt ctctactata atactctatt
30181 aaaaaaaaaa aaaaaaacgc taaacaaaac agccctttgg agttaagatt taagacacag
30241 gaagtgttaa attagagtaa attagagttt tactggataa actcaagaat ctatttgttt
30301 tggggagtgc ttttcatacc actactatgc agcaattgta ggatgggcaa tgctgtccta
30361 gtaaccctgg ataggtaaca aatgttgcaa gagtctctcc ttgctctatt aaagaaaaca
30421 aacaaaacaa aaatgatttc taggctgggc ctggtggctc atgcctgtaa tcccagcact
30481 ttgggagacc cacgtgggtg gatcacctga ggtcaggagt ttgagaccag cctgaccaac
30541 atggcgaaac cccatctcta ctaaaaatac aaaaaattag ccaggcgtgg tggtgggcac
30601 ctgtaatccc agctacttgg gaggctgcga cagtagaatt gcttaaccgg gggtggaggt
30661 tgcagtgagc caagatctca ctattgtact ccagcctggg caactgagca agactctgtc
30721 tcaaaaaaga atgatttcta atggcaacca atgatttgtg gggcttttaa aggaaagtaa
30781 ttaaaaatag ttctgtaaca ataacaaaag aggggcagct gtgggctatg atgccaaagc
30841 attccagctc ttaagtcaga tactggtgga ctctcatgct ggctcagcca ctacttagct
30901 gtggggcttt gggtaagtca ctcaacctct ctgaagttca ggagccttat ctgtacaatg
30961 aagataatca tatttagtgg ctaaatctta aggatactgt gaagactaaa tgacaattaa
31021 tgcaaattca tataaagtac aactctttta actaccattt gtcattacta taaattggag
31081 ttgatcaatc tagaaacagc tactttgcaa agtagtgacc tctctggtac tggaaatctt
31141 ctaccaagcc aggagaggac ctatctgtta aagaacatcc tgtaagaaat tcttgacatt
31201 gagttggagg ttgttctatt tgacttctaa agcccttggt attcttggaa atctataact
31261 accctgcaag tctcttctgc accttgacta tgttaagttt aaccaacttt tattgagctc
31321 caaccactgt tcatgtaaac attagcgaga atcagtccct tccattgagg gacttccttc
31381 agttggagga aaccgaaaca cggacaattt catgctaaca tggaatatca gtaatacaag
31441 ttctacaaag tttagtttca ggaagggcct cttcaaagag ataatctcac agtgactcct
31501 gaagatacaa acacggagga cttctgtgta aagactccag ccttcactct gaataaaagg
31561 cacatgcatc cctccagcca aagtgcacag gataaattta acaggcacaa cactggaagt
31621 aaaaaggcca cttcaccact ataaagcact tttttcaaaa ccagattaca gaaaaaagat
31681 ccaataaatt atttttctta atattccaaa atattaggat atttgtatat cccatgattt
31741 tatgatagat ttgtaaaaag tgagcctacc taaggcaaat ttcaaaagaa gaaaaatgct
31801 tctcagagct ttaggattga gtttctgtat cattccagtg ctaaaaagtt tgaaYtgttt
31861 ccaaaagtca acagctttgc tcaagttgtg ataaatataa attctatact tatgatgtag
31921 tgttttcctc tggccttctc gggagccaat gcttgtRtac tgtatgtggt ttagtacRtg
31981 ttaaatacac atttaaaaat tccatttttg tgaagtaaat gtgacaaatg tatgccacta
32041 aagtctttat catcaaagct ggataactca catgttttac gtgcagagta ttgagagttc 32101 aaagaagcaa agcagatttt gattattatg atgcttccag gaaaatgaat gaaatgattc 32161 Mtagtataaa gtttttcaaa gtgcttttga atagacttat atctagtttc taaaacatct 32221 tatatagcat taaaagcata attgagcaaa atatgattac atggaataaa attggaataa 32281 aattatgttc aaaggcggaa gtaaaatgca ttttataatc taaattgggg agaatacatt 32341 tgcaacactt aatttagtgt atccccttta tcgctggctt gctaacatag cagaaaagca 32401 ggccagtccg tctgctctca cctccatgca atgcctgcct tttgcaggtg tgcctcaagg 32461 acttcttctc attcttcgaa tcatcttgca gacactaatg agcatcgctc ttcagcttta 32521 attattggtc cctgacaagt caacataata ggaatcagct taaccaagca tactccccag 32581 cgacatgttc aattactcag gttgtactgc tccaagagag gtctgagggg ttgctaattt 32641 aacactgtca aaaaaataaa cataggtatc acttacagtt tatcttttga cctttatgtc 32701 accaaatcta acagtatatg cttttttttc ctcctgccag aaaagatctg cagattaagt 32761 gtgctacaag accttttcaa aaaaaaatta aagtatctgt ctgaggtcta catgatgaat 32821 gaagttacat aatgtacttt acaatctaaa aatgctggaa tttgttaagg gagtcatttc 32881 acctctttaa aaaaaatgaa aagatattct gatctgttgt ttactcctac cactttatcc 32941 cttcacatca tgaaaaagca ataatcctgt tactttaatg caaagattag taatcatgag 33001 tggaaaatca aatgcatatt aaactaagaa ttttatgcat aacttttcaa cagaaatata 33061 ttatacagca aatgaccRtc atttatgcaa tatatccctt tgttacagta cagaaataaa 33121 taaattagta tttttaatta aaaactgtag catagtaact tccttggtaa cttaactaaa 33181 cacaaccagc aaactgcaaa ctgtaccata tttgagctat atcaataaca ctcttcttat 33241 aatttttgta accatttggt tgttataata agtgttccca ttagctctta taaaaactga 33301 atctccaatt tatacaactc attgaaggta ctttatttgt cagcaattcc ccccaccccc 33361 taaagccctc ttaccttcaa actttaatcc tggcaatatc cacagtgaaa ggttcatcca 33421 agaaataact aattaaattt gacctagtac aatggtcaca gactacacac ttctagaatg 33481 ctgaaatgta aaaatgcatc actacctaat agggagataa ggtgtccaag agaataagac 33541 gaatattcac aaaagtatta catctgctac taaagaaact caaagcgtgt gtcctaatga 33601 tagtgggcca attttaagtt tacataaaac cttgattttt aaacagYacc aaaattacat 33661 atttttatac ataacattat ataaatcata atatattaaa attttttatt atgttgagta 33721 agtagtattt tttgttgctt tttaccaaga ttattgagat gtaaccttta cttgggcagg 33781 aatcttgcat atttcaactt tgtaattgca atgcccagat cagaatcttg atcatttaga 33841 gtgtctgtaa aaataaataa tgactgaatc aacaaatgaa ttatccacat tctgaatgtt 33901 taactcactc tatgtatagg tatatgtaaa tccttcaaaa agtagtttct taactaacac 33961 actaatatac taacatgaag tttaggctga ccacgctgtt aaaaaccttt ctagctagtt 34021 aaaaaccatt attgctgctt atcgtttatg ataatatatg ttattgtaca atattctatt 34081 ttcctctttg atatgaaggt agctttgata agtaagtagt Mataagtaaa tagacaatga 34141 attatttagg aRaatgtaaa tgtcaccaca acatatataa gcagcttaat tttcaataac 34201 atttagtttt ctaggctaag atactctgat gatagatatt agaaactttS attgctcttc 34261 ttctttttca cagtagcata gaggtaaata aatttatatt agactgttga atcctttaga 34321 aagtgatagc taaaatttgg aaaatcatct aaatttgtga tctgattata aatagagaat 34381 atgtggagtt catgatattc ctcatacaat taacacttca atgaatttat tatcaggctc 34441 tgatagctga gtatttgcta aacttgaatt ccaaatttat agtctttatt aaacacttac 34501 ttttttccaa aaattcttaa aatgtatttg tatactacaa gttataaaaa catttttaat 34561 tttaaagaaa ctaagagtgt ggaatgttta aaagtatgaa atattaataa acatttctac 34621 aaaagtgttt attgctttcc cattcactaa actagttcct caaaagcatt cttaatatta 34681 atagaaaagc aaacaagaga agaaaatctg tgaaactggc tcagcatttc tagtcattaa 34741 tgaataatag gggaggattt ttttctccct ttctttcaag tcctcagaga taacctaaga 34801 gtagagtccc acttgtctta aaagggagct tttcaaaatg acaggacagt cagacatcca 34861 ggccctacct agggccccac ctcataccaa agacacttaa aaagtagaag ggcatttccg 34921 ttatcaccaa atgaaatgac ccaccccttt cctagaacca atctttattc caaaaacatc 34981 acgataaaac aatgcacaaa ataaaatgat tgattcaggg tgacaaaact gaacaatgct 35041 tcattctcaa actttaaaac tgagatgcct ccatcagtta ttatgcRcaa aacagcccac 35101 acattatgac caatgaggat tgcaccgcag ttttcgattc aagatttaca tcagttcaac 35161 agcatatccc tcaaagtctg gttaaatgtg agatttatca atgtaataat agaagcagaa 35221 ataaattaca gtgtagaggc caaaacgaag aSataaggct tccaaacaga ttagaaaagg 35281 gggcaagtca atggagtcct gagataaggg cagctgctct agatggcaca ggttcagagg 35341 agaaggccct ttgaaaggcg tgccagtgtg aagagggaga tgagaggccc gggggtaggt 35401 gaggagaagg acaaagggaa gaacagggtg actgagacca gtttgacaat gagcatgaat 35461 ggaacagggc aagagggcga aaataagtgg ttctacctca aacactgagg gactctcttt 35521 ctacttccta gttactgata ccttcctatc tcttcccaaa gtatctgtgg gagctacatt 35581 ccccaaacac ccaaacccta gaagctacta actgtcaaag gcaaacacag tttaacagac 35641 acttaacaat cacagttttc acagtcttct gaatggatag ttcctaaatg ataatccaat 35701 taaaaaatat acttggtttt ttttctgtac tgctaacttc gctcttagaa cattcagtaa 35761 gatatcaggt agaatttctt tcatatcagg tagaatttca atcggactct taagattaca 35821 tgtcctaaat gtaggagtat gtaaggtaaa tgaaactgat tatttcaaca tctgtttctc 35881 acttccaaca tcaagaattc ctttgtaatt attaaaaaca agaaagaaaa cctaacagtt 35941 tttccgaaca cttccaatat gttatgaaac attaatgacc ttttctatgg attttagaaa 36001 acagatttct ttggcaagta cttctttttt tttttcttcc agctgaccca ttcccttcag 36061 acaagactat gtgaagcaga gggggttgat gaagggagga taatgacatc agtatgtggt 36121 caggtgggca gagctcaggc cagtatattt ccactatgca agccagtcgc cagcactcaa 36181 atagcacttt ctgctaagac actgctaaac ctattgcaga attttaaaga ctagcagggc 36241 acagtggctc acgcctgtaa tctcagctct tcggggctaa gtgggcagat tgcttgagtc 36301 taggagttcg agaccagcct gagcaacatg gtaaaatctc gtctctacaa aaaatacaaa 36361 caaacaaaca aacaaacaaa aaaaaaagcc aggcatggtg gcttgctatg tagtcccagc 36421 tactcaggag gctgaggtgg gaggatcacc tgagcctggg aggagggagg ctgcagtgaa 36481 ccatgattgc accactgtat tccagcctgg acgacagaga ctttgtctga aaaaaaataa 36541 aataaaataa agtaaaaaga actttcaaga ctaagacact aagtgtgatt tcaatatatc 36601 ccaactactc tacatgtgga tctcaaacag ggatggtaaa agattaccat tcaaaaggct 36661 gatcacaYtg gcaacctcta ggtgtcaagg ttcctgttgg caattattgc ttccccctga 36721 aagttttgaa attgtctttt aaaaactaca gaatgacaaa actatttttt ttaacagaga 36781 aactgatggc aaagataata tgcctgaatg gctgctttga aatactgtga tattctaaat 36841 tctgaacttt tttttactga ctgataagca gcatatttaa attgcctatt aacacacctg 36901 caatgcctgc atatcctcca tccaaagact gaccctagct ttaaatctct cggagtcaac 36961 tgtacaggtc ttgaaaatca acgtcctttt attcaaaacc atggtccttt atttattctg 37021 tgctcttttg atattcatat ggtatactgc taccatatct gtctaaaagg atggcaaatg 37081 ccattcctgt gccaattcat gtgcctgtgt tctgctttac aaaacagact gcatgtttct 37141 taggccaagt agtgtatcct actgctatat attcctagta tttttgagtc ctttacacac 37201 aaatatctat tcagcaaatc tggcaactta atgaagttgg gaacacttct gtctaagatc 37261 tccagaaatt catcatgtct agtggcattt cctatttttt atgatgctaa gtatgcatgt 37321 atgccacatt tccaagcttc ctattacctc atccctagca tttttagccc tagcaattta 37381 aacgtcagcc catgcaaaga acagattcag tgctggagtt tactggttgt attagtatgc 37441 tttcatgctg ctgataaaga catatccaag actgagcaat ttacaaaaga aagaggttta 37501 atggacttac aattccacat ggctgaagag gcctcacaat caaggtggaa ggcaaggaga 37561 agcaagtcac atcttacatg gatggcatta ggcaaagaga gagagcttgt gcagggaaac 37621 ccctcttttt aaaaccacct gatctcgtga gacttactca ctatcatgag aacaccatgg 37681 gaaagacctg cccccatgat tcaattaact cccaccaggt ccctcccaca atacatggga 37741 attcaagact agatttgggt ggggacacag ccaaactata tcactggtta tttaagaccc 37801 tgtttctcat cctcatatgt ctcaaactca aaagatggta agtcatgact acaatgaaac 37861 tttctccttt tccccccaaa atctaaaagc ctcttaatac ggtttggcta tgtcccactc 37921 taatctcatc ttgaattgca ggtcccataa tccccacatg tcatggaagt gacccagtgg 37981 gaagtaattg aatcatggga gcagttaccc cttgttgtcc ttgtgatagt gactgagttc 38041 ttgagagagc tgatggtttt ataaggggct tcccccttca ttcagctttc attcttctct 38101 ctcctgctgc cttgtgaagg acatgtctgc ttccctttcc gccatgatta taagttgcct 38161 aaggccttcc cagccataaa gaactgtgag tcaatgacac ctctttcctt tgcaaattac 38221 ccagtcttgg atatttcttc attgcaaagt gagaatgaac taatacattt tcaotggatc 38281 caagaagagg tgcaaacatg acccttgcat gtcaaatgct gttacaattt cccatcccaa 38341 tagcaccaca tgtttcaagc accatggcta ttatctgtct tccatctcag agttagggaa 38401 gagtaatata ttctagcgaa ttttttgctt gtcactgaca gtaagagaca caaaaacact 38461 actacttgta acgttcataa ttatggtaca attatgaggt gatgtgttct taaaactaag 38521 aaactctaag cttagaatcc aagtaaccag atttctaatt acgaggacag ttctaagctc 38581 aattcacaaa acagacagga aaaaaaaaaa aaaggctttg catttcttgc taacacagat 38641 aaaacattgt atattattct tcaaatttgt gagatattct gtaaactcta gtctaagacc 38701 agggataaag gacacatgat gataaaccat cttactataa tttaacttaa tctagcagta 38761 ataaagatag ttgtaattta ctgttaatat tgggaaaggt tgatgaaata aagctaaaat 38821 ctcccactgg cgttcaatca aagctcttca atttccagat gattataaat gaaaatcaga 38881 aaacaagcca gtggggaagg aatggccagt cgaggcttaa gcctagctgt tctacctccc 38941 ctagataaag aggctgagcc actaaggcat ccaggcactg gttttccata gaattcaagg 39001 ttatagtttg gcacaactaa tcttgcttct caatatttct tctcagtgct gcaaagcatt 39061 ttagcaatta agtatgaaaa acacttactc ctggaagcat aaaattcact ttgtggattt 39121 atatcttccc attattggcc tgtacttaat cactgctttt tttcatcatc tactcagtgg 39181 ggttctgtac aattaagatt tttcaaatcc ataattacaa ttatatttct gtaacatagt 39241 tttatgacaa attgcataYg cttttggagt tatcaaatta tctagaatca aacctgctag 39301 atataagaaa gcaccgtgtt aatgataaaa caactgctat aactagaaat gataaagaat 39361 ctttggaatg ctaatgtggt atttttttaa aggataagga gatgaaattt aaaaccactt 39421 ttacaaagat gctataccta aaaaaattca tatgttaagg ggtaacattg ctatcagaat 39481 acccgtggtt atcagctgcc ttaggggctc ttgaacttta ttttaaatgt atccacattt 39541 caaaatctac taaacaccat acaatttcaa gataaaacct ctgtctttta ttctttaatg 39601 ttactgcatc agcattacaa agtacattaa tatttctgaa taacaaaata cattatgaaa 39661 tcctatgaag atgtcttctc catttatgct acccagttta ttaacattaa taaaaatgcc 39721 atatttatta tcatccatgg ctggtgtgag catttcaata tgttacccag cagcacttga 39781 aatattgata caaaagtgag agtacattat tcactggctt tctaaaacaa gcttttagca 39841 ttttaaatct aggtattcat ctttgatact gcatcattat ttggataaca ctaaattcat 39901 aaagtgtcaa tcttagggtt ataataagaa gtaagtaggt gtgagctcat tctgatttat 39961 tcctaggtct cacgcaccac aaatggttta tgtttattac ccttgaatgt tcagatatac 40021 acagaagaag taactgccat gagagatatt tctgtatggt tcactactga atacccagta 40081 cccagcataa agccctgctc tcagcagaca ctgatatatg tttattgaat gtgtgaatga 40141 atctagcttc atcacattcg atgccatatg agaaaatcat tttagggcaa tacagagttt 40201 ataaatgaat taaaatttgg aatcaaattt ccaaagattt tattttttaa aaaatgtcaa 40261 attcctgtaa aatcttaccc ttgaaagtgc ccttgaaatg cactttcccc caggtgtaca 40321 tcttttatat gaaaccaaat catcaagttg ttgacttcaa taacagacaa tggatggatg 40381 aactggcctt ttaagtcttt ttatacaatt cctttactaa cattatatac ttatagaaat 40441 actgagtcat aaatgtatac atttcaaatc ttcatcttga ctttcttggg aggcactagg 40501 ttcccaaagc acaagaatga gttgcttata ctttaagaga atttaaagat gactctcttc 40561 tcagctccat ccaccttcat gttgctgttt attaatctga ttaatctgtt tgtttgtttg 40621 ttttcaaagt agagtaaaaa taaaatcaac attccttcat ttctcaaatc atttcccaaa 40681 gctagaaaga ggactactgt gggcattcaa aatctaaaaa ggctattccc acaatttata 40741 aaacaggtca ggaaactaga agcattaaat cagtgatcaa tatcaggcct gcccaaaaag 40801 gaatccacga agaaaaattt tatttagagt ccttacaaca ttaatgaggc agagaaatct 40861 ttgctgacta aagccagaag ttagcagaga aaagaaacaa agactgtttt cataaaatca 40921 taacaaccac gttgctaagg tgtaaagggg ccatttaaat atctaggtta ctttgtgcct 40981 tgaactattt tgaacattcc agggaaatgc tgctgaatcc attttaggga gtcccaaatg 41041 caaaattagc cacactgtga gaaaagttaa aagaaacttt taacttaaga aattgtgagt 41101 aaaatttgta cattggtgta aactgcccct gctcctgaac ttacaaagtt tacttttatc 41161 aactctgggt aaaatgcagg gtcctttcag aaactggact ggatgaaYgg cctggagtga 41221 tacccaaggc acatttagat ttttacaatg ctacctggca gacaccatgg taggaaaaca 41281 agtaacgatt tttcactcct ctttYgactg ctgaagtatt aaacaccatc agtttagcat 41341 aaaaatccct actgagttgg taacaccaag taaaacttca gcttaaaggc agaaagcttg 41401 ttttaggagg taaacaataa aagtctaact ctgcttatta tgattttaac gttaacattc 41461 tcctacctca ttacctctaa cacatttact ggaactcaaa gtacgaaaac cacagcttta 41521 tttttataaa gacaaaagga acattaacat taatcatgga ctggtgcaac gattactttg 41581 tcctaacaga acgctttcta tgtgaatgtt tcaaaatatt ttataaacaa aacctctgtg 41641 gtacaaagta agagaagtgt gaatattaca ccaatttttg aaatgcttat tccttgcatt 41701 tgacatttac aaatcttcac ctatattctg ggcatctgtg tgcatggctt atcttccctg 41761 acaggctgaa aacaccgtga aggcaaggcg tgcatcttgg catttgtatg tgcagtctgg 41821 ggtctctggc acacacaagg cagccatgag ctacttctac aaggctgtga aattcaattt 41881 taggaagctg atctgaacac aggacaagcc acattcttca acactagctg aatcaaatga 41941 aaggtgatat tttgttctct caacttcctt aatcttgtgt tttttttttt ctcttaatta 42001 gtttggaact ccagtaccaa caaggggtgc aatttggggg catgtagaaa caacaacaac 42061 acaataccta acactattaa gtaatggaaa tagaatttta ccctYggtaa cctttacagt 42121 aggataaatt gaacgaaacc tgctacagga ggaacgataa cctgagtaaa gtctaaagat 42181 ccatctatac tcagtgtgca tcgtcaagct actggctcca gtcatgcaca gctacgaatg 42241 catgcaagaa aaatgcaaga aaaagtaccc aacctgttct gaccaccatt aatactacca 42301 tcaagcatgt actggaatac tccaaaaact tacaacccca tctaaaaaaa aaagaacaca 42361 attgctagta aaaacaaaaa cctaggaatt accaatatga actgagcttg cagagggaag 42421 ggaagccaag caccttaatc atgtgcagat catgatcgac aaatccaaaa atatttttca 42481 aaacttaatg atgaacgaag aattacgttt tcccagtacg tgtataaatg ccccttgagt 42541 aataagcagt tataatacat caagacgata gcagtaagaa caaaacaaat ataacaataa 42601 aaagctccat gaaaggaggt atgagaaggt aaatttttgt aggcaagtat aatttcaccc 42661 tcttaacaaa cattgtaatt ttaaaaaata gaccaatgat attaaaaata aaaatcctaa 42721 ttctgtagag ccatttaaac gtacttatat caaagaggaa gaccattccc tttattttga 42781 agctttcaat agtctcttta ctctgattgt tccaatggat aaagaaaatg gttattcact 42841 ttcatcaatt ttcctaactt ttttcaatat tttaaagaag ttgtttattt aaaaaaacca 42901 catttttaag ttttagtact taattcaaat ctgtaattgg aattatcaag aattgttccc 42961 tcttctcttt tccaaaataa tccaaaactc aaaacagttg gtcagtgtga ttgccaggaa 43021 aaaaaaaaaa aaagcttaaa caatactgaa ctgcagttta tataagggga aaaaatgttg 43081 ttgaatttta gtaacatgat ttgttttaaa acaattttaa gactaactga atatagacta 43141 gtcaggctga acaaaatttt gaattttttt tcaagtttcc aagttcaaga cactgcgctg 43201 ataagatgtt ctttttctat taccaaacaa tatagtgtac ttacttaaac aaaaggagaa 43261 aaaaaggagc atatatatca aaggagagat caaaggagag atggcacata acttttctac 43321 cttctaagat aatgtggata taaaccatga aagaacgcat gcctactccc cctacccaca 43381 gtgtctgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtggtgtt 43441 cctctctctc cattagttta ttagattggc taggatttac attaaaggtt gctgatcacc 43501 taaggcaaat agcatgatcg agtagattaa atgcaaagca accagcctca acaagcacca 43561 cacaacatgt ggattatgtg aatgaaacac ttgtcttctg tactgacaca ctctacccac 43621 tagcaatgac tgtataattt ctaaaaaaga aaaaacgtcc cacccaacaa acatgcatca 43681 ctaaaaagaa ctgcaagtcc ttttaaggta gacagtaagg ctttcggaag tggcgggtga 43741 Rggtgttctt aggaagattt ttttcttcta cttttctgtt gtctgcattt tcttattcac 43801 cacttagggc acaggcaact tgacactagt ttctgtaacc aacctggtcc agcaggattt 43861 ctcacacacc ctgcacaaaa ggctaacccc ttacaaaata cttatttcag atttacgtca 43921 aggttcaatg aattgcagcc aggaaactga gctccaaacc taatataaaa gtctcctctg 43981 ctaatcacta aaccgatggt tagccagaag gtggggaaat taaaacttta tactgtttca 44041 tttcctctca tgcaaatgta aaaagagaaa cactatctta gagaaattta tttttcttca 44101 ttaatttata tggaaaatct atgacttcaa agtgaccaca caagtttact ttaacaatgt 44161 aaatcttcca tgggagccaa ccacgtgatc tttaacttgt attcacaggc cctcctaaag 44221 tctttactgt actcacttta agttcccaag ctgtcataat gcttaaactc cagctgaggt 44281 attaggtgtt atgataactc atgcaatgat aatgggtact gctagagtat acaccttcta 44341 agggaaatca atgcagtctt gggtttattt aacattgcca gaggtaaaac agactcagga 44401 accagtgaat ■cagttaccaa gaaatctcct gattctgcca ccgttggaaa aaaagaaaag 44461 tgcactttcc tattctttca tgttcccctt tatgccccac agaattctca cgagaatcag 44521 aggtatccta aacattgttc agttcttgca gtgaacagtg ctcaaatctc actccaatta 44581 cctccagtta tgaactgagc ctgccaacac cgcagaaaga gtcagtggcc aggaatttat 44641 gtcaaattac acgttacaca atgatgaata ggagagaaaa atagcagcga ttaaatacaa 44701 tactatgggg tacattatta gatttgcaat aaagatacaa ctatgctttc ttgactcaat 44761 tgtacacatt ggaaaagatg tgccgtagct ttcacagggt attttttaac cctatattaa 44821 gtgcctttaa tacttaattt tattttttat gctgtggccc tctagctata cctgtttttg 44881 tttttcaagt aaattgtacc atagatcact ggtaggggaa acaaaagcaa aagcaaaaca 44941 aaacaaaaac aatagatcct gatgacacag gtctatttat acaaacgatt gaagcaaaaa 45001 tcaattgtaa ctgtatcagt ttatgcaggg agaaatgaca attctatgtc atgtgactag 45061 acaatatggt gacagatggg tttggaaagc ttcaaaataa ttggtgtaYg tttaaacagc 45121 tcatagtgcc cattacacat accgtatggg ccgccaatta tttctttcca gtttctgttg 45181 ccaaaatgct gaattcaacc aaccccctca caatattggt accccaagat ttctgtccac 45241 tcctgtagca gacttggatR aggggagcat gtttatctac agcaagtagt caaaatacag 45301 gagttgctta catagttgtg ttcttttcag gaataatact tttctggcca ccatccacac 45361 tcatggtata cttgtgaaca cattaactcc actgaatgtt acattcatag gtttataaat 45421 aatggtacat acacttgaaa agactgtcat taaaaagcca aacacgtttt tcttcatacc 45481 ctacactata tatatagggg cttcgaataa acatgtaggt ttccaggaga gagttgagta 45541 gatggagaat acttagagga aaataataca taacatgtta ctgccccgtg ggaatagaga 45601 ccttaggcct ttattctgat ttctgatgtg acaactctta ggtgtgaaac aaagactcct 45661 gctgacaagc agaaagcagt taaatgacac ataacgatgt ggactgtttg ctgaaagtag 45721 tgctgacagc tacaggtgtt tttctttttt aagtttagaa ggccctcgga agtggttcta 45781 caaccctgtc ttttagcagg ctaccctgca aggagattca aaattatttc caaaacccca 45841 actgtgtatc ccattggtaa agtctgacag catacatccc tctcaaaaga ggtggaggaa 45901 catctgaatg gttcatattg ccgagaagac atgaattaga taaatgtaat attagtggtc 45961 tctttgtatt taattgcatt ggaaaaaatg acaggttttt ttttttgttt tctttattac 46021 taaacatact ggagttcatt gttttgattt tattttccat ttgataagcg tgtgctttca 46081 tacatacatt agtgagttcg ccaaagtact acaaagtaac cacattttaa acagaaaaaa 46141 aaagaaaaaa attccaccca attttctcag cttctttaat ttggtggaaa acgtaaagtt 46201 gtctctccaa ggtaaactag ctgagaaagt gattctaaga gtaaaagcaa taaaatttaa 46261 ccatagcttt actagctgtg ctcacaaatg aggtaactca acaatgccaa ctaaaataaa 46321 tatttactgc agtaaaaata acagcaccaa gaagaagcat catgctacat aattctgatt 46381 acaccaaagg caatatttct tatcacacca aattaaaagg acgaaagaca tttacttgaa 46441 acttcaaggt aagggaattt ttaagttctc atatgaacaa cactgtttac ctaatcttgt 46501 accatatttt catgtaagtt atcctcatac catggcatag attttagcaa attcgtggcc 46561 atatcctatt ttaaagaaaa ctgtacatat tgaattacca taacatcttt gatactaatg 46621 tgcatgggca aaagttaagt atgagagaaa gaaagaatat ctacatgtag tctgttcatt 46681 taagtcttag caggtggtca gaatgttgca tgtatcataa aattctggac aaccccccag 46741 tctacaagat ggccttgata gaagagccta aagtcactaa acagattgcc aaaaggcaag 46801 tggtcagaat tgtggacaga agctacccta cagttccttc aagcctgcag caacaagaag 46861 aatacacacc tgttgctcaa ggccagttgg caaaatagaa aatggaagtg atgcagatct 46921 agaaccttgc tagtgagctc caggttatgt ggcactaagg gaggggcagt gccaatggca 46981 tggcttaggg aagcaatgat ttctgagttt tgctgttgtt tttgcatttt tttctttctt 47041 cttttttttt gtagccagct ggtattccta aggtcagaaa aacatatgga aaatggcaat 47101 attccagaaa tcagaaaaca gaaaaaaaaa cagaatttga ttaattttgt cactcattct 47161 tcaaaccacg gacaatgact cttcactaag taatggcaac ttcaacctct atctgtaaaa 47221 atttaagaca gctacaaaag ttctaataaa ttgggataat cacacatttt cattttgcct 47281 cgaagtttag tgagatgata tgttttcaag agatctgtaa ttaaaaatgR tttagtgcct 47341 ataatttcat caaaccattc tgtgaaaggR aagagataag atggaaaaat gaagagaaaa 47401 attgatgcaa gaaactaagg caataatact gcttcaaaac ctgataggat aaatgtaata 47461 atcattctaa taccatatat gtttgtagca tttatgtctc tgctttttat cctaaataaa 47521 cccacgcatc tttctcattt gagaaaattt acattgcttt ccctggaata tttcttgcca 47581 actatggcag tttacaccat Wgagaggaaa gtctggttaa caccaagttt taggcctcag 47641 gtatatccaa gatatacttg ttccagatac agagagacag atgaataaaa agattcacca 47701 catgttgaaa taattggtct caaaaatgcc atcaagggaa taataaatca acatggacag 47761 aaaaactcag tacatcaatt cctagttaca gacatatttt ttccacatgt tgattcttca 47821 tttctacctt catttccatc gtaatctaat gaaattctaa atggacacaa ttacttctca 47881 ggtcattcac tgttattaga aaggggggtg gggaaatctg gcattacctg tccacatttt 47941 caggtgcact gtccttgaca cagcaattcc acttccagga atttggcaaa gaaggatctt 48001 tgccaaattg atacctctct ataccaatac caaatcttcc taaatattca gatatggatt 48061 caagacaaag gcaagaaaca gaatttggaa tgagcacttt tgcagtgact taaaatttct 48121 aaatagcttg ataacataaa atattccaaa tatctaaagc aaggatcctt gtcaagaagg 48181 tgccacccat caggtcacac caacaccatc tgggcagaga aggggagccc atgccaggca 48241 cctctatcaa tgtggacttc tcatgtgctc cttggtccat ttctgtccct aacgtgcaat 48301 ccactatgtt aatataattc cctgtgcctc ctgcagctct ttcagggcgt ctgtgtagta 48361 gagtctaaat cctctttcag cattcatgat atatcaggga ttagagtatt gaatacagtg 48421 atcttctagt tgtaagaggc tatggtttca acacctctca tactgagagt tttaaaccag 48481 ttgtgttatt ttggggcaac ttacttaact tttctatacc ttagtgccat caataaaatt 48541 gagttgacaa tgcccagcat cataatgtta tttggaggat taactgagat gatgactgtg 48601 actgacagtg actgtcctaa ttcctgaccc attactgtac ttcttcccag aaggcagata 48661 ccatgtatta ttgtactccc ccagcaaaaa ccaaatgcca gacacatttt atataagaat 48721 gaacaattaa tctgaataat aaaaataagg cttatttata aaggttaagt tgaatagtat 48781 atacatgcac atatatgcaa atatatataa ttcagatatt ttatctttct taatgcttac 48841 atgactctaa actctcacaa ttttaaatta aaacataaat atgacataat tgtaaatttt 48901 tcagctttat acatcagcat tttaccatca tgagaaaaga tattcttttt cttctttcta 48961 atttccccaa agttaaattt ttaaaacata gtttactata aaaaaKtatc aacaaattag 49021 cactgaataa attgttcttt atttctagtt aatttatttg aaaagttgat attttatgct 49081 ctagtttctg gcatgagata taaaacgtgc cactgctcta aaaaatatat cagaaaattt 49141 ttttccacaa atggcttttt taggaaaaaa aattataatt aacaggatca aaatttcaca 49201 tagttccaca tctctaaact tatatagaaa tttaatgatt acataacttc aaccatatca 49261 gcaaatacac ttcttttact agcatgcatc aatatcctcc caattaaaat actctgtaag 49321 actgcctaca gtatttcaga tcaaaatagc ttttactgaa atagactttc ctgcctctct 49381 gatttagcat ctcactttct cttattggca gatggttagc caagttctat ttaaatccat 49441 atgtcttcca taggcatcat acaaatactg aattaaaaga tgaacaaatg ttttaaagct 49501 aatcagatta gcctttaagt gaaaaaagta acaaagtgga gtttcatttc ctgcaaatga 49561 cttttactac atcacggtta gatgactgaa ggagttacat ccccgtaaac tacccagaca 49621 aaaataactc actgtatgag acaagattac agaaaaagac tttcctgact ttgattattt 49681 ttttcatgaa tccttcctct ctgcatgaat tccaatcctg gcaggcaata aatctggtac 49741 cagagtgata gtatagtgta ggcatggttc accatcaaga ctaagaagaa cagaagagca 49801 gaatacttca ccagctttat ttccatacag ctataccctc tctccctcct ttacttccac 49861 taatttgcta aagggtcaaa taaacacaca agaattccca cttgaggaaa agagctccat 49921 gcctttatag atttcagttt cataaatata acctgtactt ttattcatct cttctaggca 49981 aagagattaa gatgatgagg ttgagtttgt agtacaaaca tcatatgcat tcctgagttc 50041 acaaaagctt tcaagttctc gtatccaggt ttgtctctag ttaagtcatg ctgcagactt 50101 aaggaataat aatctactag tatctttctc ttcttcttat cacactttct gggctatatg 50161 cctttgcttc tctgacttgg ccataaaatg atatagataa atggtcccat tacacatttc 50221 tggttatctt gagcccagta tctaatattc taaaatatct cccttcttta gaaggaagca 50281 gtatattggt atggtaaatc attcttcaca tttttgtttc aacacttgcc agcactgtgt 50341 tctatataaa ctaataatat agctgtgtag aaagaagact gattaagttc aaccaaaatt 50401 gaacctagag tcaattttat taaaaaaaga aggtatataa aaaataaatt ttggagtcat 50461 gatttaacaa atagactttt taggtttgaa ctgaaacttt taaaacctag aaatgcactg 50521 gcagattgtg tctctagatt cctgtgtcac aaaattctat aaagaaatct ctgtttcacc 50581 agataactca gaaaaaaggg atatatatag atatatatgt agatatagat acatgccaag 50641 tgttaaaatg ttccattaaa gctcaaatcc tttttattgt tatacgaacc ctaaattgct 50701 gtaattgatt ttttccagag taagtaactc actagataat cttataccct aaaggttatt 50761 ggaaataatg cacaaagtaa taatttacta attcatcagt cctaaaagag tatacaaata 50821 ataatcaatt tataattata atcttaatgg tagaaaattt acacaggcgg tacggcagtc 50881 tcatacagca aataaaatat ggttttccaa attttaaaaa atgcatattt cctcatataa 50941 ttctattcca ttaaagatat attgatttta gacctcctaa aagattagca gtataacagg 51001 gccaacaaga aacaaaaata taaaaatgca cagccctcag gagctctcag tctgctaaag 51061 gaggaaaatt tgcaaatgga taaattaaaa gaactagtat ttgcagtatc tctcatatct 51121 ttgttctact gtgtcccata cttgtctaaa agctccttgc ttttagtgga aaaacctcaa 51181 aaaaaacaca cacacactca cacacacaca cacacacaca cacacacact cacacttcaa 51241 tgaacttcaa tttcaagccc agggagtatc acagaaaccc caacattgcc atgaaattga 51301 taccttaaaa tcataagaac tggaaactac atgcatgcca actcaatccc atgtaaacca 51361 aaaccaccat gtttgaaact cagcatgtct gttcattaaa aatagaataa ccaagacttc 51421 tgaccgaacc agaatactgt gcagtcagtg ttgccagcca taagaaaggt cagactagct 51481 tgggctggtc cactggatca atcgtctgga ctgggcctgt aagcccaagt gtaaactcca 51541 actgtattgt tacctatttc caagactttc acccactata tagttaSgtt agctattatg 51601 acacaagtgc agattgatta tgattagagt tcatagtcct tcaagtttgg aaataactac 51661 ttaaaagcaa tctagatata aaacttaaaa ggtttgttta aaataatgga aaattggtat 51721 actcagggta gttcctttac ttttaaaatg attataatac atcttcagac aattttcaaa 51781 agaactacca taaattctgt gatcatcaca caacgaagtg tggctgattt ttKaaaagac 51841 attttaattt gtacttatta taacaaaact tttaataagc atattttttt cttaacatta 51901 aatgggtaaa taggctagta aaaagtcata aataggccta tctctcagat ccaaattgtt 51961 tacaagaact gaaagcctta aagcttggaa gattttaatc aagacttcca ttaaccccct 52021 accaatatcc cagggatcgt aggaatttta aacaaaaggg aaagcagaat gcttttaaaa 52081 tgacttaatg tgatacagta ttaagatatg taacagtgtt agcgattaaa caacaggtga 52141 tatatcacag Rcaagcattt ctcttttttc ctcaattata aaataagaca ttaaacaaga 52201 cttgaaaata agccactggg ttaaatctaa gagaaatgat tagcgaataa atcagaacac 52261 caatacataa aactaaaatt tgatagttct tatatataat gaatctatcc aacaggcata 52321 gtaaatacgg cattcatttt taaatgggta ttcaagaatc ttttcatgta tatttttttc 52381 tttgataata tgtatataYg accctaagta gtagggctat tttttattta tatctgaata 52441 aacgctattt acatatatac acaccctaca tatatattta tatatttaaa agaagataaa 52501 cgttttgaag ttatttacat aattaaacaa ttaaattttt attttagact ttgatttaaa 52561 tacccacctt ctgttctaaa acgtactctt cttttagttt agctggttac aataagatac 52621 ttaaagcatg catgtctttg tggtgtttct gaatcctatc aattacgaaa agactttgac 52681 atacccagag gatcttaaaa tttgatctca caaatgtcaa aatagtaact ttcactgcag 52741 agggggcctc actcattttt tttaacatag atttttagtt ggagtgaaaa gttcaacttc 52801 tgcttagatt ctctgtaggc tagtgtacta tgtttcttaa gctttccaaa taccactttt 52861 caagaaccaa ctgataagca acctcttctg gaacactgtc ttgatagatt aattgagaag 52921 gggtcatttc cttttttaaa tttctacaac attttgctat tttttttttg ttattgttgt 52981 tgttttttct cactctgtgg tacctgacac tctttttttc ttgtgctgta atcggtttat 53041 tgaacattat ttttgctact ggtttgcaaa agtcttaaag aatcagtgtc tatagcttag 53101 tcatggtaat gctgctccta taatattctg aacaaactag gctttcagtt tatattcaat 53161 tattaaatga atgttagtta atttggaagt cattccaact cctctattcc ctatgacagc 53221 ctacgtttcc agctatccat tagatatttt catctgaatg ccttacaaac ttggtaagta 53281 caaaaccagc ttcattattt taccctcaca gaacagctga ctctagaatt ctgctaatga 53341 tacactgaaa ataaatatac gccagactcc gtggctcacg cctgtaatcc cagcactttg 53401 ggaggccgag gtgggtggat cacctgaggt caggagttcg agaccagcct aaccaacaat 53461 ggagaaaccc cgtctctact aaaaatacaa aattagccag gcgtggtggc gcatcccagc 53521 tactcgggag gctgaggcag gagagtcgct tgaacccagg aggtggaggt tgtggtaagc 53581 tgagatcatg ccattgtgct ccagcctggg caacaagagc gaaactccat ctcaaaagaa 53641 aaaaaaaaaa gaataaatat agtaaagcgt gtgaactttg aagccaggct acttggacat 53701 gatccagttt gatccagcca ttcactagct gggtgacttt tgacaagtga cataacatct 53761 ctgaggctct acttcctctc tgtaaaatgg agaaaataca gcatctatat aaaggggtta 53821 taatgaggat taagtaagtt actacaaaca aagagtaaag ccttgcccat agttagtctt 53881 tatcaaagat tagctattat ttgtctcctt tcagtcatca aaactcaaag cctctttttt 53941 gtcacccgca catataaact ttgctaactc ttgaagactg tctgctcagc atagctacag 54001 aaatcacttc ccaacatcat tatcctagtg taggctccct gttattactc atagagtaag 54061 agatcagcct actaatgagt cttcgtcacc tcaaccccgt ccacctcctt tccatgtcaa 54121 cctatgtaag atggtcatat taaccctatt aaaggtttca tcaaattatc ctttgcccaa 54181 atcctggaat ggttccctat ttccttctga gataaagttt ttcaaataaa cattttagaa 54241 aattatccta acctttatgt caagtcttag ctccccatac tatactatac ctaacttttc 54301 atcaagcttt tctctttcct atatcctgaa aatgcccctt gtaatcttac attgtttctt 54361 ctatattggc ttacccttca tgctgtcata ttcacctgct aaaagtccag tcttcctttc 54421 cgaaacaagc tcagaaaacc ttttgggaca actaagtcgg ttttctcaac acaatgtgat 54481 ctgttcctct ttaaacaatc ataatcattc tcgtctccct tcacctcttt tatggaagtc 54541 tgattgtatt ttagctgtca ccatacctgt cttatcacct acagctaact gtatataact 54601 tgagtacaga aatcttgata tactcatttt catacccttt ataagttctt ccacagttcc 54661 tgcctgaaac ccagcaggtg ctaaattaat atttatcgga caacaagaaa ctttagagac 54721 cagtatatca ggaattaact cYacaaagtt atcctctgct ttttctattt ctatttcttt 54781 caagtttgag tacaggttag tttggtctag tgaatatctg ttctttgaaa ataaatcctg 54841 aatccatatg aataccaata tatttaaatt cggacactca ccaacacgca ttccaatccc 54901 acaaacagaa gtgtgtcttg ttaaaatttc atgaactacc aaatataatg aatctctagt 54961 gttatgaaag gactttgcct agagaagtct aatgcttgga gaagcaactg aaaagagaca 55021 ggacatagaa tagttttgta ctctttgctt taacacctga ttttcattct tatcttacaa 55081 taaactgtac aaatagctgt aagcaaatga aagaaaattt aaatatttca aaattcagtt 55141 tgtgcaccaa acacttcact gagtagttac tggcatcaac ttcatgtgct caccaaaaca 55201 cattaagaaa tttgacatat taagaaatgt tttgaaggca gttaaatccc atatatatat 55261 ataactttat aagtgcattg tcctatgaaa tattctctga atgtccaagt gactgagaaa 55321 tctttcaatt atgaaagtaa aatcaatacc tggctgattc tttgctgctg ctcaagaaat 55381 gcaccttatt caaataatca tattcacttt ttccactata ataatgttta aaaaacttca 55441 gggtttctat ttcttagcta tcactgcaag gcaattcaaa ataatggaag ccaagaaata 55501 atactgtttt cctgtttctc aggagtctat atacatgatc tggtaaagtg acctatgctt 55561 gccaataaac tctactacWg tgtggtccca tagaggagcc tgactgttgg gacgccaatt 55621 ctcaaaattt aaaatcgcaa gcaagttgca attattcagc tataaacata ctaaaagtgg 55681 cactattgat aatagcaaaa aatagaaata aatgtccaac aataagaaat cgatagacaR 55741 tttttctagg acaactagta caacatatag tgaaatacta tgaagtcaat aaatatgatg 55801 tagcagaata tacgctgcta taaaacagcg tgtaatttat tagaaagtga aattaaaagt 55861 tatttaataa taaacataca aatctatgtt tgcaggtgca tgtctatgtg tgtgagtgag 55921 catgtctgag tgtgcatgtg cctgtgtgtc tgtgtccaga agtaaataca ttaaaaacgt 55981 tgaaaactta ttttcttctt tttgcttgtt tatattttct aaaatgtcta taatgaacat 56041 atatcttgat aggaaagata atgccaagaa atcctaattt aacacccttt tctcttttcc 56101 tggacttttc ccaatttgtt tctccactta atccctagcc tcaccctaga ggccacctcc 56161 tctcctgaaa cccacaggag ctaagtagaa atttggtcac tttcatcatg atcacccata 56221 cttcaaataa ttcatatatg agtaatgaca tatgatctct gaaggaacat aaagaacaaa 56281 gataggttta tagttttaag atttcagtgg gatgaaactg aattattcac atccattaaa 56341 cagagtttga atgattcaca gccaatttta tttttataat tgtaatgaaa cagctgggat 56401 tagagtctat tcatggactc ataagaaatt tgcaccccac ctactaaaat tagtttctgc 56461 atccaaaatt ctgatttgaa catctgactg tgaacaaaac aggcatcatc gctcaatgcc 56521 aagaaactga ggaaggagac tttaagcatg tcactttctc atagaattat tttctcattg 56581 tgcttacttt cctttaactt cattcatMag cagccccttg acatgattct tttgcagcaa 56641 agttgtttcc aaagaatggg ctttcttgta caagataagt tcaaataata caatttttga 56701 agctttcaga ctgtatgtgt gacagttgct tataacttat ggattctcag ccactggttg 56761 gaaatcatat ttaagaattt gtagttcata tatatatata gatatagata tagatataga 56821 tatagatcta Katctttgga catcagcatc attacgtcca aaatataaga ttttgtctta 56881 aagttgcctc gggtatcaat tcatagatct gaatgtttaa ggttttaaag gaaatttact 56941 tgactctaga tttgcttgac tctgcaattg taaagagatt tgagaaactg catacttcaa 57001 actataatct tccaaagcag gaaacagtat atgaggcttg agtgactcac ccagggtcac 57061 acggctagtt agtcacaaag tgaatctaga acaoaaagtc tcctttcctc caatccagag 57121 cactttccac tacatcacaa gggaagagca aaaagaaaaa ataacatcta attttaccag 57181 aaaataaaat acaggcaggc ctgaaccaca gccagagtta aatgtggagc atacctacct 57241 ccaaacccag ctggaacctc aaaggttagt atatatttta gactcttcaa aaatatttta 57301 atcacatgtg aatttgagga aagctctgat tttaaaaact caaaaacact cagtagtcac 57361 acagcataaa gttatgacag tattagctgg ataataaatt tgtagaccac aaactgtcca 57421 tccagacaat ttacacttta aaaacatcca accttcttag aattagtttg taagtcaggc 57481 aaagaggaca gatttttctR tattaccagg gaactttcag gtaggcaatt tctgacacta 57541 agatttagat aagccaaata tgtgcatctc ctaaaatata gccagtattg ccttcaatta 57601 taccatgatt ttgcaaacaa atatacttct aaaatgttaa caagtaaagt ttttttccac 57661 tgttcaatgt acaaaagagg atgtYtcaaa agtYccactc tgagtctttt tttaaaaaaa 57721 tagcaaataa ttatttttaa gtaaagtgaa tgtctctttc aaatgcagtg tttgtacaWt 57781 tttagattcc atatagaaga gtggcttaac ttgtttgtct taaacattca taaataacat 57841 catacatctt tttataaatg tatggttaag agtggcctga gaatctgtWt ttttagcata 57901 taggtagcac aaatgcaatg ttaattcaga aagtatYctt gcccctgctt cttcaaattt 57961 tcataaaaaa taataaaaat attctatagt tagcaataat atattgcata tttcaaaatg 58021 gctagaacag aggacttaaa atgttaccag cacattgaaa ttctgataaa tactcaaggt 58081 aatggatacc ccaaaaaccc tgacttgacc attatttatt ctatgtttgc agaaaattcc 58141 cacgtatacc tcaccaatgt atgaaatatt atgcatcaat aacagaaaaa aagcatattc 58201 tgacattctg acaactttat caaagaaaaa tacttaacgg aatcaatgtg catttaatat 58261 atttcagcaa aaaaataaaa acaaagataa ttaaatgcca tcttttaaag aaaagagaaa 58321 tcaattcttt tctcttgtac tcYctcaaag cccatgccat tacccacaca tgcgacatac 58381 atcgggggaa ggcgaaaact tcaaaactgg tttgacaaag cgtgaagggt agcaggagca 58441 tcatccatgg aggcaaagca aaagcagaga aaagagagaa attattcgcc cgagaaaggc 58501 aaagcctgag tagactgctt ttaattaaaa cccttgactc aWctctgctt aagaaaatga 58561 gttcttctta caaatcaatt tctctaaaga ataaactata ctgcatatgc aatataaagt 58621 aagcttatta tactatttac cttgtatcgg acatgtactg tattccatat atgtgataaa 58681 attgccactt gcttttctcc taattttttg aatctatcat taaatatgtc aatttacaat 58741 ttagaagtta atccttaaat atttctagga tatttttccc acgcattttt tataagccaa 58801 attattttat accttaaaaa ttaccccttt agatagcagt gacttggacg ttaaatacca 58861 tttgttataa accatgtact ttctcattta aataactgga aatatactac atgaaattat 58921 cccaaaaaga tgataaagca atagaataat atgtacaagg aagtccttca aaatgtcctt 58981 tataattaaa aattggaagt agcctaattg ttcattaata aggaatgtct taaacatact 59041 aggatgtcac tattccatgg tataatgagg cacaaaaatg aacattgtgg gatgtatttg 59101 ttgatgagaa aagatgtcca caatacactg aaaaattaaa aaagaaaatt ataaaacagg 59161 gaggtacatg atcctatact tgaataaaat atatatttaa atatacataa aggatacatt 59221 agatgccaaa atatgaacac gaaagtattt acaggttgga atttatgagt ggttttcatc 59281 ttctttacag tatattgtat tatccaactc tttcgcaata agcatgtatc acttatgtaa 59341 tttcaaaaag gtattttgtt ttgtacatac acacataaaa acgtgtatgc ttagatgaaa 59401 caaattaatt ctgaaactgt agaaagtcac ccaagtattt attagcaaac catattgaac 59461 caaaagaggc tatgcaatgg ttatgagcag ggactctaga attgccagag ctagaattta 59521 atgtctgcca ttataagcag tgtgatctag gcaaaatact taatatccaa gcctcagttt 59581 actcatttaa atgatgggga taataatagt ttcaacctca aagagttctg gtgaggatga 59641 aaggaatcca tccagtaata aattaatgga aaggtagtaa gcttaatcct agaaactgaa 59701 tgctaaattg acaaccagtt ataaatatcc gtctatttta accaaatatt ttttgtaatg 59761 ctttcctata caattgtttt aaagtagttt caccagattt tcttttcttc attaagaatt 59821 caaatttaac acctacaaac aggtattctt gtgttaccca atgtatatag ctacaatcag 59881 aaagttaatt ccctgagatt ctatgtgctt tgtctaccaa gacttttttt atgatcaagc 59941 atcagtcttg tataaaaatt taaactaaat gtaacactac aggcaaatgc aattcaataa 60001 atcatgttgt cagggatctt atcacttcct tcacacaaaa tatcatttta accagaaatc 60061 tattttaaaa atcttttcca ttttgtacct aaattctcat aaaagtttga ttcataatca 60121 gtaccttttt taatggtagc aaaaattata aagtgtattt tgcctttaaa aatagatatc 60181 tatagtgtat atacttagca ttttctggtt attcatgatg ttacacatat ataaatttcc 60241 atggtagaaa atggtttatc cttttcctaa ttaatatgtc caaagtaatt cttcaaaatt 60301 aagaaactgc cttgtttaaa agaggcaagt taacatataa aattacaagt gagagggagg 60361 catttaatca ttctgagatg aacaatgatg ccaaacgctc aatttatcac aagtgtgctg 60421 tcatcagaaa aaaacaaaac taagattcct ttgcttcact cctaaccact gatataaagc 60481 tgtgttccag acttccttgt atttgtaaat tatagttcat aataaaagtc tataagtagt 60541 attgcttctc caaaaggaaa aagggtcttc aaacatcaat gaaacaattt gcctttcctt 60601 aaactgataa cacaactaga tacagcagta cctagcttgc caagcttcct tgagttaaca 60661 agcagagcag tctactaatc aatgcctttg cttacaaaca cttaaggaaa gttttgaaca 60721 gtatctaatg gaagggagag aagaaaaaaa aaaaatatat atatatatat atatgagaga 60781 caatggagat aatatttacc tttttggtga aaataacaaa atccccaaaa atccaaaata 60841 aaacctcgtt gtggcatttt aggtattaat gtgcctgtaa aatggaccca tcagactggg 60901 ctctgccgga aaattaccag attcacatca acactacata cctcattttt gaatcattct 60961 ggccctgtgc tcctgaaact tgggccacct tctgtatact cttcacttcc aacatctaag 61021 aatgctcaac aatgggttct tttgggggcc ctgttatgtc ctgactcatg cagtccatgg 61081 aaaagagaag ccaagcaaaa gatagttata taggaaataa tgaacagata ctgcactaaa 61141 ggataacatt tcacagtagc tgcaaaccta cagtccaatt cactaagata catctgcttc 61201 acctgcttta gaaacctggg ccagcaaaac tatgccagac ctcacaaaca gcctaggtta 61261 caaaaacatt gttgttgtcg tttttcaagt gctattaatg tgggtgggag tgtaaactag 61321 ggcagccact atggagaaca gtatggaggc tcctcaaaaa aaccgcaaac agagctacat 61381 acatgatcta gcaatccctc tcctgagtat ttttccaaag gaaaggaaat tagtatatta 61441 aagagacacc tgtaccccca tgttcactgc agcactattg acaataatca agatacggaa 61501 tcagcctagg tgtccaacga cagataaatg aataaacaaa atgaggtaaa tacacacaat 61561 ggaatatatt cagtcataaa aaaagaataa aattctgtca cttgcagcaa cgtgaataga 61621 acaggcgaac gttattttaa gtgaaataag cctggaatag aaagttaaac actgcatgtt 61681 ctcacttata tgtggaagct aaaaaaaaaa aaaatttatc ttgtagaaac caaaaacgga 61741 agagaagata ttagaggata ggaagggtag ggggaaaggg agaataggca gagatttgtt 61801 gaaggatatg aagttatagc tagacgggag gagtaagttt cagtgttctg tagcactgta 61861 ggacgcctgt acttaacagt agtatgtagt ttcaaatagc tggaatagtg aatgtcccta 61921 atacaaagaa attacaaatg ttgaggtgat ggatatacaa attaccctaa tccttccacc 61981 acacattata tgtatcacaa cagcactatg ttccccacaa tatgtacaat tattgtgtgt 62041 gcattaaaaa aaaagtgctt taaaaaatgt gtgggttaat gaagcagact tatagaaaag 62101 aaattagatt gcttaaaaat ctcattaatc aaactaataa aaccaaactt ctatacctat 62161 ggaatatgga acgaatttga caactttcta ctgtcaaaaa tggtatataa atgtaaaaga 62221 tgaataatca ataaataaga aaaagctact tcacttttac acggtaaaac agaactgtca 62281 tgttcactat tacacataat gaatttgata catggattgt atttttaaaa tctaaactaa 62341 aacatatttg agtttaaatc atatattttt caaatagatg tcaacagaaa tacatctata 62401 tggcacagaa gcagagacaK gtacaaggat ttttcatctc tgcatatagt caatagtttt 62461 taatcattta ttttactctc aggcaaagaa aatatcaact gctacctaaa gagaaactac 62521 caacagggcc taaagtoatg gcttcataac cagtgtttag agaggtatat acttcccatt 62581 tttcctgata tagtctgatc tctttggtat tttacttatg cacatgtaaa aagtccaaat 62641 cgggtgagaa aacagttatg tacataaaca ttaagtcagt gctacctctt tgataactgg 62701 aattttgctt tcattttaat taaatatgaa gcaaaacaat gaatatcact tttcacttca 62761 gaccatcttt agttaaaaaa aagctcctgc agtcttctgt cagtggttgt aaaggcattc 62821 aacattgtca cttgtttaaa cgtctgtttg gctgccaatg gactgtcccc aaaagaacat 62881 ttttgcatct caatagtctg tccggtatca acaatcttta aactataaat ttaacgctgc 62941 tgaagaaaag gggaaataac attctcattg tactgtcctg gtatcacact tacaaaatga 63001 acaaataatc agactatttc cacttatcta gcactctgca tctgaggatc tcaaatggcc 63061 tgacaaacgt taattaacta agctgcacaa cactcctgtg agggtggcag gcagcatgat 63121 cctctccaag cgacagatga acggagatcc tgggaggccc ttcctttgaa agtcagtggc 63181 tcaacttgga actcaacttc cagaacttgg aactcaactc cccaggccca tggttgagca 63241 tctccgctga agccaagtag cacaaaatgt agatgttaca actgacagac ttttctaaag 63301 aaatttaaac tggaaaccaa gatcaatctc aaggagattt atctttcctc tagctgcaag 63361 aaaaaagtgc tctaataact acagcatttg aacctatgga agaacctgag ctacaagtta 63421 tctataacat tccgttttga caaataacat ggaggagttt acctgaatgt tgattttcca 63481 tgagctcccc ctaaagactc acccaataag cttaactgta cttccgtatt ttattaacaa 63541 atgaaaaact acacaatgtt ggttaatttt tttgtgaaat ttaatgatcg atttttggat 63601 ccttattcat gtagttatca gataaaaagc caagttttct gcaatggaat atccaatcta 63661 aaaattccat tacaaaaata aaatccagaa tttcttattc aggaaacaat aaaattaata 63721 cagttgcatc tgcataaatt tatctaaaaa ataaatacct ttgtgtagat gtacatcata 63781 aaaaaaaaac ttgtcagaaa agtatacaac tttacatgaa tgctgactat actatactac
63841 ttagtttaaa atgattaaga aacatctatt ggaatatata aaataattta cttatactgt
63901 tggtacaggt aattttaaac atgttaacct cttcacagac ctaaacttta aataaaaaca
63961 ctgggttttc aaaatattat tagtaaatgt tggtaaccat tttcaggagg tgattcatgt
64021 tctataagat ctattctaga aatgttaaga taataaagtt gtcatataag cttagactat
64081 ttctagcaaa gattctaaag cccttaatac ttcttcctac taacacaaga aaatactcaa
64141 aaattttgta gaaatattgt gtgattaata tgcaaaaaaa agcctagttt tcctagagca
64201 atttataaga tcaagtttat ggcaatgtgg cttttgtcat actcaaagca cagaagtcaa
64261 cacagaggca tatgcaactg aaaattttta tatcaagatc aattccatac taataacaag
64321 ggaaaaatat agacatttac taaataaaaa tatacataaa tgatatcata ttgaacacaa
64381 aactaaaagc caaacactga ccctgacata agccaaaaaa aggagaaaac cactaatgtt
64441 aatgaagaga acatttcgaa attttagctc ttcttgtgat tcttatattt ccttcctcac
64501 tttgagaagt acagcaaact ttcattgcta aaaccagttt aaaaaaaaaa gttatcagaa
64561 agactaatat gctgaaagca acattttaat tatgtgcttt gtaacacaat cctaactaca
64621 ttcagccttt tatttcaaaa tgtttccatt atttaccagc atagtattga actatattaa
64681 atcaacattt tctgttgggc aagttttcca tacttaattc ttgatatcac tctgtatagt
64741 atttttaaat gattaagttt tactttagta ttagaaactg tatatataaa tgtattaata
64801 tacttgctct attaatgcta tattgaaaaa actatttaat taaaaaggta cttttaaaat
64861 ctgatagttt acatttctta aattgcttac aatttctgaa taattgtagt tactttcaag
64921 ttctatttta ttgtttcaaa tataaacatg cattctgtag gcatatattt atacactact
64981 gtaatttggg ttcaaaacat gtaaattata tctgtatatt tctatggtgt attttaaatt
65041 attacaggac atcttcacta ataactatgc ctctgaaact ataaacttat agtacttttt
65101 ttttggcctt tagtaaacat gacctcaaaa ttctgctttt tttttttttt ctaccactgg
65161 tagggtaatg aattagaggt aggtaattcg gtatgataat ttcagaatac caactaatac
65221 caccaaagta gaattggtgt caaattcaca atgagaatgg gctgtttctt aattctgtta
65281 ggtgtcttaa aaagctcatc atctaagata tgtacacatt Raaaatttca tatacagcta
65341 ataacctaaa tgttgcccaa taaattaaat tggctaccct tcctattcca aatatccaRt
65401 aaaattggtg atttccaaaa gcactattca aatataaaat gtgaatctta aaagatttaa
65461 attttattgc aaccacagaa tgattcaata ttcttccttt tatatatgca tatgcatgta
65521 tttgtatata ttaatgtaat tttatcagat ctctgaaatt tagtagatta cagcctttga
65581 agataataaa tcatagggtt ggaatttggc tattattgta gaacacagct aaggaatctc
65641 ttgtttaagg tttcctactt cagcagctaa tgtggaggaa ctgaaaaggc tgttaaaatg
65701 atgcaataga aacttgaaat cagggattta aggaagtgga ggaattgaga aaaacaactt
65761 tttagaaaag tattaacatc tccatcttgc tcaaaaaatg caaccactac gctcaggttt
65821 tttataccat tgcttaataa attgtcattt caaagtctta gagtatataa acaatggcag
65881 aaaaggtaat attccagaaa cagtcaaaca atatggYgtc aaatgaagat gtcatggatg
65941 aaaccaattt taagtagaaa aaggaaggaa aggggttccg gtgatataga tataaggYcc
66001 ccaaatatac catactctca aaatgcagta tgaatttaat aaaacatagg acatttcatt
66061 cccgccttat aaccagaagc catgagtcac ttgaggccag aagagtaatg acatgctgag
66121 aaaacaacaa atattgacaa tgataaaaat gcagctccat gtatattcac tggaggggag
66181 ttccctaaat tgaaccccct cttgaagggc acagaaactg tttctctttt tttcccgaag
66241 ttactcctaa taaacttgct tcttccctgc aggaaggatt ctttgtttct ctctgtacag
66301 ggggcatctc ctcttctgta ttcaagtcct tctcttagct ccagggcatg actacagatg
66361 ggaaagctaa cagcttctat aattaactgt gcacctctaa ggggcgtttt tcaactccgc
66421 tagagaatac tgagaagcac agataggctt cagagaatct gcaggaaatg agagcagagc
66481 agccaagcag acattgggaa gagcaatttt tctccccaga atacatccag agagaactca
66541 ctggtctatg acccgcaggt caaagcacct ggctaatgga actctatttc ccaactggat
66601 cttttgtaaa ggttagctgg tttctgatcc taccttctat gcacaaacaa agcttcaaaa
66661 tcatccagag aagctcaaaa ctttcacaga cttaaggtaa aatgtccaag aggtggtgtc
66721 ttaggcatcg agcaactatc acttattggg agctcatgaa ttttcaaaat ctaaaatact
66781 ttttaacaat gctgagtcta catagtatga agagagcaat cgaggatctt accttactca
66841 gaggttttgt tttctttttc cacatggaac taggtctttc cccctaaaag atcctgttca
66901 tatcaaatgc atggcactct tctttggccg cttttcacac tattcacact tagccttttc
66961 tcatttttcc ccctcttgtt ggcaactcga caacaacaag cattgtcatt tatacataat
67021 tatatcgtgg gttttaaaaa actccgtatt ttggtatgaa catctcatca aggacattac
67081 attcttagtg atttaaatct cttctgactt ccttattaac catatattgt catagcagcc
67141 taaaatttaa aagtcattta gttatttcag tgtagcagat gtacatcctt ctgggattct
67201 aaacctccct tctccaaata tggccttgtt gttttgtata gtcaccagga atgattaacc
67261 aacattcaat ctgagctaag agcaaagaca aacaagtgac tgcctaacac tgtatcaagt
67321 aagtgcacag cacacacaaa cacacatgag ttacttgctg ccactttggc cttaggagta
67381 taaatcccca gattgagaca gatcattaag tttcacttca cacacaacac aatcctgtaR
67441 agaaagaatg tgtgttcctc cttttgccct tcaaaacaaa Kgaattattt aaaaatgaaa
67501 ccatcctacc cgtaacaggg actgggattc atccttggac ttgttttctc ccgatgcagg
67561 atactgctgg gggagggccc cagacttctc tccgccagct ggcgccccct gcaggaatcc
67621 cttggtttcc acagccaagc cataaatagg tcgcgccaga tgggcggcct ccacattggg
67681 actatccctt agagtctttg tctgctcttg ggtgccagac acaggcgtca gcagccccag 67741 gcttgcttgg gtgtatgacg gactcccccg caggatgtct gcccctctcc aagtcacatt 67801 gcgaaggtca tcactggaac tctcggtcca aactttctct ttgagcccgt ccttctcttc 67861 cagcttctct ctcttcacca cactctcaga aactggctct cccattttag agtctggagt 67921 tagcagattg tagaccctga agtcaatttt gggctcctct ttgatggtgg atatggcatg 67981 accgtcctct tcgccgttgg ctgtagtgat gtcctgttcc tggcagtgaa cagtgttgaa 68041 gtgctccagt agtgactgag tatcggcagc tgtaaaactg cactgacggc atttgtagca 68101 gctgtgtgct ctcctggaga agaagaaaac agttactgca aagacagcgt tctgaagggt 68161 tgttttattt ttaatagcct ttaagcacct gaattttcta cctactcatg caatatagta 68221 ggtagaaaga aatgcaatat tcttaacacc ccaaaactgg aatgtaatta acaggcattt 68281 tcagttctat ataaagagtt caaagtgccc ttgttatatt agcagtaata ggtataacac 68341 agacacacac acacacacac acacgcatcg tgcacgcacg cacgcacgct gctcgtgctt 68401 gtgcgcatac attccaggtg attagcatta aaactggtta gtttgaatta ctgttaaatg 68461 ccccactctg ttcattttgc cttgatagat gcagtttacc ctgatggata atcatgaatt 68521 atatcattta agtcactatt cttaagtttt cacacctgat ttaaaatgcc cttttcactt 68581 tctgttattt aaactaaatt gaggattttt ccccctgtag atacacttca atttctaatg 68641 ctgaaatgct ttgtacagct tttccatata ttaaaaggaa ctaaatttaa atataaaata 68701 ataaatcatt atcaaattat taaatgattt taaaaaagaa ataaagtgtt cttttctatc 68761 atcaWattta aatccattta gtgaattaaa tattgctcac tgatctgtaa agtctagaaa 68821 ataagatgta tttataaccc atgatttttc tctaaacaaa ttctatccat ctatgagaac 68881 gtaaacacat tcaagaaatt gagtgtctgt tgagtgtcaa acaccataat agaggcttta 68941 caaatatatt cattaaaagg gtgtgtgtgt gttttctttt taaatggcat aaaacaagac 69001 aattttatta tcaaattccc taccatcttc tgctgttaaa gagagaacag tgactgctac 69061 attagggcaa atatttgatt aaaaaagaag aacagaagta ttgcactgta aaaagcatac 69121 ccgaaagata cacaagtgat gtggaaaaag tcatattttg tttaactata aaaactgtgt • 69181 atggctgtct aaataaatat cagggaaaca ctttctactt ccctgatata acttgctatg 69241 aattaccaaa ttaaagtaac aaaataaatt ttgtgagcta ttacttaaag ctgtaagata 69301 ctgtaagtta tgtactcata catgtaattt tagataatca tcaaaatatt tccttgccag 69361 gcactgggta cacattggtg aataaaagat atatagtctt tgcccacctg ctgcttagag 69421 tctcgtcagg aatgactaga caggaagttt cacatgacta taagtctatt aagtacaaag 69481 agtgacaggt gctaagaatg aaatggatag taattggtat aaagggctgc gtaggtaatc 69541 aggaaggact ttctgaggga gatatatgaa atcaaagggt gaaaagaagc tatccttgga 69601 ggatgaggaa ggggaaagga agccacattc tagctagtct gagacactga aaagaggaca 69661 ttgtggtttt agaggaataa gtgtgaggaa gaatgtcaac tgacaagctt gaagggttat 69721 ctagagcttt gtaggtcatg gtagggacag gggatctcgt caaaggtaaa ggaaggcaat 69781 cagctgggga gtaggtgctc aaatgcatgt tttaattact ctggggctgt aatgtttatc 69841 aataacttca ttcctttata agacatgtct gatggctgaa attccaaaag aatgttccta 69901 ttttatggaa ggaaaattca aaggtataaa acatgaaaca aaatctcaag tatttgttga 69961 gagtgcttaa tgtagcatac aagagcttac attttgttga gtaaatatcc atatttactt 70021 tttacagaag attgtttcca atcttttccc tatttttttc attgtttaag tagtaaagaa 70081 atatacaaca aatgacaaat aatgggagga tgaattgacc ataaaagcac aatagtaaaa 70141 ttaattccct caagtggaaa cagcctaacc tttaacgtga agacgattat caacaagagg 70201 aataatcttg ccaccaatgt caaggccaga aaattcaaaa acaggactaa gaatacaaaa 70261 gacaatagac aatacctaaa atctaggacc tagaacctct gtggagctca tctacaaaag 70321 cttcttatca aaaacacatt ctaggcaggg cctggtggct cacgcctgta atctcagcac 70381 tttgggaagc agaggcgggc agatcacctg aggtcagaag ttcgagacca gcctgaccaa 70441 catggtgaaa ccccatctct aataaaaata caaaaattag ctgggtgtgg tggcgggagc 70501 ctgtaatccc agttactcgg gaggctgagg caggagaatt acttgaacct gggaggcgga 70561 ggttgcagtg agccgagatc acgacactgt gctccaacct gggcgacaga gcgagacgcc 70621 atctcaaaca aaacaaaaca acaacaacaa aaaaaaccca cacacattct agtttaatct 70681 aacgggagat aaacacataa atccaaaatt accctactga atatattcta cacaatatat 70741 tgagtcatgg aaattaaaac agcaacaaca acttctgaaa gcacttaaca aaccatgatt 70801 gattttcacc ccaagatggt tataatggac tcactgtcca ctttagcata tttgtggttc 70861 cttgacatgg tgagatctct gcttgaattc tctaaagaaa tccatgccta taaattaaaa 70921 ctacaaatta ccactgacac ttctaccaaa actggtagct ccagtgataa aatgccaaag 70981 aatcatagtg attctgtttc aagatatcaa gactgtaaat accagggtga caagaaggag 71041 aagggatcaa catatatgaa aagaaataca aaattcttgt ggaccacaaa gcaaaattat 71101 tttcaaacgg tagactctca gttcttactg gtgaaatgac caaccacatg taagcgtgta 71161 gactcttaag gagaatgctt aagcttcaaa ctttggctct gtattctaca atgtaagtaa 71221 cttcgaataa tttactgaac ctgtctcagt ttcctcatct gcaaaatgaa aataaagttg 71281 tctcYtgata tggttcttgt gcataataag taaataaatg agagttaatc agtataaatc 71341 ctaaaattaa ataaagcccc tctgttctct gtgaatccac attcYgtact gtttctgcaa 71401 gagtttaaac ttacaaactg gtgttttggt atcattcatt gccattcccc catgtacctt 71461 aaccatgacc aaaaaaaaaa aacattttta aaaccaagct tatgtcctag aagatccttt 71521 gctttaggac atggcccagt agaatatatt ctatgctttt ctgcttcatc tagcggcgtt 71581 taatgacatg aaagaatgag cagtttagcc ttttactgct taattgtcaa gctttactct 71641 tttttcatta atagcagtca gagccagtat cttcaaattc taaataacaa agacagtaag 71701 ggcattacct gtgttttatt aaaaccttgg gaggtaagga tgttttatga atgtattcat 71761 taaagttgtt tagaacatgg catatctagg aatatagcta agtaattaag attatgttac 71821 ttcaatgtct actgattctg aataaagatg aaggaatagg ggtcgcttaa gaatatttct 71881 agattgcaga atcagtagat attgaagtta cataatcttc acctattatt taactagtac 71941 ttcttcctaa atatacaagg tcctaggtcc ctgcccaaaa catgagttca tcacagggta 72001 tgacaccctg agacaaggtg tctgagtctt ctaggttttc tagtttgaag ttcctgtccc 72061 aaaggaaaac tccctgactc ctggcatgtc tcagcaggag ctgctgtcat catcctggag 72121 aggacctcgt gaaccaaccg acctaggcaa aaccgcccat ggtacaggtg ctggagagct 72181 gtaggaacac agagaagagt ggggatagga ataaagtaca aaggaaacag aaagtgcagg 72241 ggtaagtttt ccgtgagacc caggttttat gtcgaaaagg aaaacacatt ttaacggaag 72301 gatattacaa caaggttgta aaaacaagct caattcacac ttcttttgaa agttcaaagc 72361 actcatgtct acaggttctc atgttccact gaagtatacg gcccatttca cagatagtaa 72421 aatggagaaa gagtgccttg cccattttat ctatatcaag tcaagaacac agggcttcaa 72481 actcccagaa caatgttata tttttctact ttccacttcc tacctttcta attttatata 72541 aatgttaact actgacgata tgccaaatga aaacacaggg gatggaaaag gtcttctaac 72601 agagaaaaaa gaaactgact acagcaatgg tttgcaaaac aggctgggca gcattgcaac 72661 ccaaagagtt tctttctcag cgatagagat tccaagtctt tcttatccca ctgaaccaga 72721 atatcctggg cataatctga gaatctaaat gtttaacttt aacaaagtcc tcaaatgaca 72781 ccaacgcagg gaccagtacc cataaactgt attactagct acatcatggc tctaagcaac 72841 ttaatgaata aactcacaag acatctccta actcccaagg ctttaataaa atacaggtaa 72901 tgatcttact attattgtta ttcagaatct taagactctg ggtcttacta ctattaatga 72961 aaaaagagta aagcttgaca tttaagcagt aaactgttaa actgctcaat ctttcaggct 73021 attgaacacc actaggtgaa gcagaaaagg caatttgcta taaagtgctc ttgagacttg 73081 aacaactcct ttctccttat attatcagtt ctaactgaaK gacagaaaac atattaaaaa 73141 gacaacatta ctgtcaaaag cctggagcaa tatgaacttt tcaataacct atactaaaat 73201 ttagaccagt gcttctcaaa ctaatgtgta aaaatcacct ggggatttca ttcaaattca 73261 tattctgatg ctggaagaMt agggtagaat ctaaaattct gtgataattt tttatttcaa 73321 attttcccag atcctgataa tattctgtat tttttacaag gtccccagac cacaccttga 73381 gtagcaagaa tcatgcagct atggtcataa atagctcatg acaagtgtgt tggcttggag 73441 tgtactctta atatacctgt atatattcag caattttaga acattcccaa ataaattttt 73501 ttttttttaa gaaacgaggt ctcactatgt cgcccaggct ggccttgaac tcctgggctc 73561 aagggatcct cccacgtcag cctcctgagt agctgggatt agaggcatgc accaccatac 73621 tcaggtccaa gatgaatttt ttatgaatgg caacattgtt atagttttta agtcacatac 73681 accttctgag tcaacattca cagctttata aaataaacta caagcacaaa attgtcagga 73741 ataatcccaa ttcatttttt ctttatcatt gtttggagat tgtctttaaa tattctttag 73801 tgccaggtca tttacatata ttgcccgcag caataacaca aagtatatat ttccacccca 73861 ttttgcagat aaagaagtgg aagcacagaa gagttaatta acataaaaat attgaaggca 73921 aaatagaata cttttgctct tgtctatggt tactgtttca cgtacgttat ttgatagtta 73981 aagcaatggt tgttcaggaa ttagtaccta aaagatttga gcatattttc tgacatgtat 74041 cagtctctca ataataactg acaagatctt caatgtaatg tcacacttcc aaataataag 74101 actagattat aataaacccc agtaaaagaa aacttttctc aagaataaaa gtttagtcat 74161 tgtgaaagta cattaatttg gactctcctc caacctacta aatgcacttt cttttcatca 74221 gcattaacat gaaaacttca aagacattca ggaaattttt cctctatttc tcaagaccca 74281 acttaaccac ccccgcatcc ataaagactt cccaaatgat tcaatccaag aataaatcta 74341 gtgtcttctt cgttattcta aatccttgta ttcctcttca atttttttaa tcacagatgg 74401 tatgtgatta aaatcacata tgcctcactt tacaagtata tgtaattacc ctaaggtcRa 74461 gaccaaagct cacacatcta ttgattcccc tcatcacaaa acatccagat tgacagtaag 74521 tatctgctta ataacaaaga aatggacagt cacagctctg gatgttcagg atgctggatt 74581 taatatgtag ggtcacaaag cagcagagaa atattaagat gtacacagat attttgcaga 74641 tggcatattt tgagtgatcc tatgaataaa cccttcatta gttaaaaact cctaaaacct 74701 ctgggtacat taagccaaac taatttcaaa ttttttacaa atatatgctt tgtattaatc 74761 taaatgctat atcctcctgc cacactgaca gcaaacactg tcttcatatt atctgagaaa 74821 aaaagatgtc attttataga tctcattagc ataagcacaa ggtcatcaca taaaacacag 74881 aatgctcagt taaatttaaa tttcagataa taaatgaata ttttagtgaa tgctgcataa 74941 aatatttggg acatactttt ataaaatctc actgtgtccc atacaatatt cgacaatgtg 75001 gaggcaagtc aaagaaattt tgat±aaaaa actaaatcat gctttctctc taggcaaacg 75061 acatcaaatt gcctaaaaag atcaaatttg tatttaattc aagtgactta gaacctctgt 75121 gaaaaatgtt attcttatct actttataga attttcatgt gccataaaaa agcatttgtt 75181 ttttgatcct tccatgtttc aatgcacaat agtctttatt cacaatagta gtatttcatt 75241 cattagtaga gaatattatg aattattttc ataattcata aagcattgag gaatgcttct 75301 agaaaaaaat cagctaaaag atgctactcc tttactatgc tctgataata tttttatttt 75361 tataataaac caaccctgca gctgagggat gccttgtttt ttttctttta aatgttaact 75421 tgagatttat gtcatcactt gaagagctct tttttactag ataatagcat gttactttca 75481 gtaatttttt taaatcttaa agacaatcaa tgaataattt taaccacagt ttttattctt 75541 caatgactaa atgaagcatc aaagtaaatg aataaatcat aaatactaat tgattagact 75601 ttcagaggtt ttttttggtt attacagaat caaacaacta cagataaatt atcacagaag 75661 tggggaaaag ggaggtgatt caaatactta aatttaaatg tagttcaagt aggtctgaaa 75721 tctaataaag ttagtcattt ttgtaatata tgcattgttg ctataaatgt ctacaatgag 75781 tggcatgtgg cattactgcc actgtaaaat gataaaatga aaaacgagaa gagagtagca 75841 ggaacaaaga caaaaacaga attacagatg gattattagg tcagtccttg agaactgtaa 75901 actagttaaa agaggagaaa tagtctacag aacataatcc aagaaaaatt gaaattctgg 75961 atgaaaataa ttatgttgtt tactcatctt gcatttttaa gttgaaataa tctgattagg 76021 atttgctcag taatagtaac tggcttttgg ggacatggga tgaggtgctc acatcttcaa 76081 gtggtagaaa aaacactact aacaccatat cttaaaaatc aaacttagaa tcccctttca 76141 aaaggtaaac attccctaca aaatgttcat gtagaaagtc tttcaataaa atagaaggta 76201 aataaggaat aggaaattat ttatacaaat gtcaatggaa ttgaaactgt ggctaaaatg 76261 tatcttattc caagacttga taactgttct tgcatagtat tcagaagcat tagttccgaa 76321 atttctaata aatcatgtat acaaataaag gtaatattgc tttgtgaggt tttatttgtt 76381 tgtttgtttg tttgtttggt gaactataac atcctgggta actttttgtc tcatagtggg 76441 tatttWctaa ttgaactatc atgtttattt caaggtatta taagcaactg atttattagg 76501 tattattttt aaaaatatga cacataacat acacaaattt aaatgtaaga tagagattga 76561 gcaggagcta aattaatttg actacctact gcaaccaatg tcaactcttt cagtctgatt 76621 ttacccattt tacgtctgga agttgcaagc actgtttaaa aattttaaaa tgcattcgta 76681 aatctcctaa tacacagtct tgtcatcgca tacactacca aaatatgtgt gtagttttcc 76741 acattttagc caacaatgaa cattttaata tttgtactag accataatta tattactgag 76801 ttKgtagtag ttctttcaca gtaagttatt taatagtgag attgatcatt cattcatata 76861 tttacttgac atgggcaatg atgagaattg atgcattttt taattttaaa gctgtacact 76921 gtatgttagt gacatacaaa taaataaaca cacacatatt catttacatc taagttcgca 76981 aacaaattaa aaactgaagc gattacagaa tcatagaatg tgattttaca taactgaatc 77041 gagatctatg cgtgtgtaga gaggaagaga ttctatatgg atagatatag atatgattac 77101 atatgataaa gaaagtacta tttatggtac agtagagtat atgtatatga tatacatgag 77161 tatcatatac atatatactt atatgtatgc atacatatat acatatatct atgtgcatat 77221 atcatatgta tgtatacata catctatgta tgtgtatatt tcagatgtat ttatccatgc 77281 atgtacacat atgtatgtat atgtatgcat gcatatgata ttcatatgta tatcataaac 77341 atataacatg aaaatttgta tagttttatc ttttgaattt atataaaaca ctatcccact 77401 gtgacttgtc tttatcatct gtttttattc cgtcaaaatt attcttataa gattcattta 77461 tgttgtagtc acttgtcatt tattaaattc actactgtaa aacattccac tgcttgaaaa 77521 ttctacaaat aaattaatct tttttggaaa gacaatcaga gttttttcct gtttttattt 77581 tggactgact ttgttttggt tgttttgttc tttctaaaaa atgcaattat gaacattttt 77641 gcacgtttcc tagcaaacct atgaaaaggt tttcttctga aaagtaaaat tatgtagtca 77701 ctgggaatat gaacatctga tattgccaaa ttttgccaaa tttgccttcc aaagtagtat 77761 gttccgacca tttctatccc tcataaggtg ttttatcgta aaatctatgt tttctgagat 77821 taataaaggg atataaaggg atctctttct ttttgtttgg ttatgattgg ccctgtataa 77881 ctttatcaat ccttagcttt ttgtcattga ggtctcattt cattgcactg agtcaaagga 77941 gatgttctat gagatactgt ttttttgaca tttaatttct ttttggctta atatgtctca 78001 atttttagaa ctgctctata tgtgcttcaa aagcatgtac attctctaat tgtttggtac 78061 agaatttgta tatttttatc aaatcaggtg tgataatttg ttgctcaaat cttttcaatt 78121 ttgaaaaatt ttttgtgctt aacttttaat aaggaatttt cctaaaactt tatcattgtg 78181 agtgtggtgt gtcatgtttt ccctgtagtt cctctatcac tttttgcttt acttatttgt 78241 atttatgtgt tatgtattta gaaccattgt actttctgaa gaagttaaac ctcttattat 78301 gcaatgatcc cttctttcct ccataggcta tttgtaataa attcttaaaa ttgtatgaga 78361 ttaataaaga tacctcaatt ttcctttagt ttatatttgc tggtataact aagtctattc 78421 ttttcttttg aacatccaga gacttatgtt tcaggtgtat aYacagtatg caggatacag 78481 ttacatcatc tgatatcttt tcagtggaaa agtttaacaR acacaggtta agacttttga 78541 tatctacaat ttttatagat tttactttaa aatgtctaca tgtctaacac ttcccacata 78601 ttttctcacc attttttaaa aagttgattg aattttatgg gtgttcttta tttcatcatt 78661 ccattttttc tcctctatag ttttggaagt tatgcattca tttcctattc tttcattagt 78721 tatccttaaa tttttccaag tatgtataac aaagtcctag ctcgctaact cccaacaatt 78781 caggtgtctt agaatacctt aattctagtc attttccctt caaacttaca tagtagcact 78841 aagcaatacc ttagtctttg tcgcccaggc cagtggcatg atcatggctt actgcagcct 78901 cgacctcctg ggcttaagtg atcctcccat ctcagcgcct gagtacctgg gaccacaggt 78961 aatcaccact atactcagat catgttttta ttttttgttt tttttagaga cgagatcttg 79021 gtatgctgcc caggctgatc tcaaacttct gggttcaagc cttggcctcc cacatgctgg 79081 gatttcaggt gtgagccact gtgccctgcc tagtctgttc ctttttgaac tacataaaca 79141 agacaggcaa tgtttgtttg gatttgctca gatgtttccc tggtcttcac ttatcagaaa 79201 atgtctttgc tttgctctta ttcttaggag atagctttga tgggcagaaa atacaaggtt 79261 gactatttga tgttttttcc aaactctgaa taatattcct ccttcctcca gtttccattg 79321 ttgctaataa aaagtctgcc aacctaaatg tcttttctga gtagtcgatt tcttgttagt 79381 ctacagctgt ttttaaggat gtctctgcct tggcattctc atttttgcta caatgtgtgt 79441 gtaactgaat ttattcctat acatcatcct gcttagtgta tgctacgctt ctttgtatct 79501 gagtatttat gtctttcatt ggttctagaa aatctttttc cattttctcc tcaaatactg 79561 attacctact ctctattctg tacattttct ttcttctggg actctgatta gatgtatgtt 79621 acaatagctc attatattcc atatcttact aaatgtcttt gatattttgc ccctccttgc 79681 tctctcttaa atattctgtc taatgcctca ttttgattaa ttcatttgct ctctttgtct 79741 aatatgtagt ttaatcaatc catatgtttt aagtttcgag agttatattt ctcaattaaa 79801 acaatgttat ttgttaattt tttggtgatc ttgatttttt tttaatttta ctttaagttt 79861 tggggtacat gtgcagaccg tgcaggtttg ttacacaggt atacatgtgc catggtggtt 79921 tgctgcacct atcaacctgt catctaggtt ttaagcccca catgcattag gtatttgtcc 79981 taatgctctc cctccccttg cccccgactc tccaacaagc ccagtgtgtg atgtccccct 80041 ccacaccgtg tccatatgtt ctcattgttc aactcccact tataagtgag aacatgcggt 80101 gtttggtttt ctgttcctgt gttagtttgc tgagaatgat ggtttccggc tccatccatg 80161 tccctgcaaa ggacaccaac tcgttttttt atggctgcat agtattccgt ggtgtatatg 80221 tgccacattt tctttatcca atctatcatt ggtggacatt tgggttggtt ccaagtcttt 80281 gctattgtga acaatggtac aataaacata catgtgcatg tgtctttata gtagaatgat 80341 ttataatcct ttgcgtatat acccagtaat gggattgctg ggtcaaatgg tatttctggt 80401 tctagatcct tgaggaatca tcacatagtc ttccacaatg gttgaactaa tttacactcc 80461 caccaacagt gtaaaagtgt tcctatttct ccacatcctc accagcatct gtttcctgac 80521 tttttaataa tctccattct aactggcatg agatggtatc tcattgtgtt tttgacttgc 80581 atttctctaa tgatcagtgg tgatgagctt ttctcatatg tttattggcc gcataaatat 80641 cttcttttgc gaagtgcctg ttcatatagt ctcatgttgc ttgatgattt tttaattgta 80701 acattcatat ctttgaatgc ttattagtat tttataattg aaaactcctg tatctgaagt 80761 gactgggtat atacatctat tatttgtgtt ttctactgat ttcacacatg atggcttatt 80821 ttcctgtcca tatggtgaat tttcgtaata gtgagctcat gtaagattta tctgcatcta 80881 tcaatatctt ggaggtaaaa tttggagtac ttttctacag acaaatttgt gtttaattat 80941 gccagaagtc agaggaagaa ctaatgtaaa aatcctagcc acctttaaga tctaagctta 81001 aagcaaaact cagattcgtc cccttctctc tatgagtaac tccaggctta gtcttcagat 81061 caaatgcaac atcgacattg gcatttgccc tcagtgtaac ctcacattcc aagcttaccc 81121 ttcacttctc cctactctgt gtttagtgca ctcttgatga aagggagaac aaaggctcca 81181 aaaatttttc ttactttgta gaagtctggc aatgcattac aaaatatgtt ttatctagta 81241 tagttgtttt gtagggaagt aatcctccta ctcgtgccaa aaacctaagt tataacactg 81301 aaaattttga taagaaaagt agcagttaat agagagcagg tacacagttg aattaaatga 81361 taaataataa aagaaatgca gtgagaaaac cattcatggg tggaatataa taacccagtt 81421 atagtctgag ttaggggtat tataggtaaa tactaaacat taagtgaatg tactaagtcc 81481 ctgttgctga ctagatcatg tttgcagttc caccaagttt aacttagctc ttaaagaaaR 81541 cacccatgca acagaatcga cactgaggtt tttcactcag agctctagat aaactcttct 81601 gcttctcagc tatagtcatt ttatagctaa atgatttcac atattataca gttcatggct 81661 tatgctaatt tttactacac cattaagtct tctatataaa gcactcaaaa gcctagaata 81721 tttcacagaa ataacacaga attatggcag aagtttccca acatgaataa tctgagtaaa 81781 caaattaaat tataaagata ctacgtgata aggtaaactt gtcctcttat tttatcttaa 81841 aaggggagca tttttagaaa atgctttgat agcagatcaa gttctaaaca tccacgtgac 81901 tatgttcttt cacatctctg gggccactat tactgttcag ctttcagtcg agtttcttta 81961 tcttttctac taagctgtga cttctgaaat tgtaaggctc caaatttcaa atatctctat 82021 aagaactcag aaacagatta gagataacta ctcagcacct taaaggatcc ctggtcagcc 82081 aaactatatc aggatttaaa cataagtgca agaatatttt aatgccctat ggacaagtgc 82141 tatttcaatt tttagctaat aaaatgctga gaagttgact taaggtcaaa atgaacagac 82201 aactgtcatt tggcatggaa gcctaggtcc tcagaagggt tccaagacca tataatagtg 82261 aagcattaaa atgtaactca cctggaaatc tcagcttatt cataactact gtgaagtcaa 82321 aaactcacag ggatctcatt tacataaaga tactcattga actcccatta ggcatcctgc 82381 cactgtgtta aatcgtgcag atacagtggt gaacaagaaa agaatagttc tcttttctct 82441 agagcttact ttcatcactg aatattaact aaattctgac attattcagt aatagtttta 82501 ttcatatttt atttcttggg ggattatttt gtttacctaa agcatgtgaa aaccctgttt 82561 tcccagacat tctcaagcaa tgcctcagct gcatcaatag aaaaaccact tgtccaactg 82621 agctggtatc tagatgtatc agaatgagtg cgtgtgcgtg tgtgtgtgtg tgcgcacata 82681 cgtgggtgtg caggtccaag atgtgtRtgt gttctggctg gtttcctcat gattaaagaa 82741 aatcatgaag atttctcaaa aaaaaagttg Yccttgtcct tttcaaaaaa agaatggact 82801 atctgatttt tacatcactc aacaMatgta catgcacaat gtgcacatac acacatatcc 82861 acgctagtaa agctcagaaa gcagctgatg tYtgcgagcc tcagaaataa attaacatgc 82921 agctaacatc agaggagcca gaaacactca ttgcaagtgt gaacacatat ttgatgcagg 82981 actgtgtctt tcccagggct gaccttataa attaaacagt gtaaatctca agtaagtgtt 83041 ttttgaacat tttgattcac tgtgaacatt gggatgccct tcaaattaac atctMacatt 83101 atgagcctat tgttggacat gaactgcttc attactggtg actgaaaaaa catacacaca 83161 cttgcactaa cacaaaactt ttaaaaggag tggaagaaac tgtttaatat catgggatgt 83221 agaaattgaa tagatYttta gactagcata ttggtatagt aagtaaagct ttctactttg 83281 atgctgaaag gcataataat gagaaagtac ccaatgagca ctaaataata cctaaacgca 83341 gggtaccacc aatgctttgc tgaactaatt cctatttata accaagtctc agtttaaatg 83401 ctccttcccg aggaagtaat ttcctaatct accatattag gttatgttat cattatattc 83461 aaacacactg ccttgtactt catcttttat gacacttatt actcttgtaa tcatctaagt 83521 gtctcatctt ccctactaaa ctgtaagttc catgagggca ctgactatgt cttctcttgt 83581 atattcaatg cctggtacaa taggattttt gaaatcaatt aattaaagaa tgaatgcata
83641 catgaaataa agatacatat cctatgctta tctctagata ttataaaaga ttaactggga
83701 aagttaatgt ttataaagag aatagagtga aagcagaagg aaaacacaaa cWtcccacat
83761 ccatttaaga atctggaata actcaagcag ttttacctgc ttggaggagc aagtctacat
83821 ccaaaatcac acatagtaca aaaattctca cctttagatt actgtttgta tgctaaacaa
83881 ggatgttgca gaattgttca aagccagaag aatatataat ctcattttaa aataaaaatg
83941 agcaacaggt tctcaaaatt tttttgcaga atagagaata ggatatattt acaatagcat
84001 ctttcattta gctctgtcaa aaaaatatag gatgtggagt catatttagt aaggataaat
84061 aaatggacta cggtaaatta gtaaactcat cagcattatt agaatcactg tgaatttaaa
84121 aagacaatca gtaaatggta taacacaagt atgaaacatg agcatacagt ccaaaagaag
84181 gaaccaaatt aaaatctaca gtgatcacag ctgtaactat atcacctgca aatctgtgat
84241 gaacttttat gtggtcttct cctataactt ccccaagtca ttggaatcac tctcaacaat
84301 tcccggttca gccctctttc tatggatttt ttctattatt ttatttgtat attcctcccg
84361 accccacccc ccatgcctca cctgtagtgt cgggaaatct cttcttccac ttgggtaata
84421 aaatcacatt tggtacatga gtgttctttg ctttccttga cagactgctg cccatccgat
84481 ccttgcaggt gatttgcttc ttgtttgaca tccgatgctt gggactcatg cacactttca
84541 tagtgaaaga ggagtacatc tacgtcaggg gtggtgaatg aacactgatg gcactgatgt
84601 ttgactcgcg agcttccagc cgccccagga gacaagtgca gaagcaagag tgcacagtgg
84661 gaacaattac tttttctaca agcaaacgga taagtaattt ctccaaggtg cttttctggg
84721 ctgcaaagtc ctctgggaca gaatggacag tgtttaatgg tacacttgtg aatgttatgg
84781 agctgttgat aatgacggag aagtggcccc actacaatta catcagggcc atggcttttg
84841 gaatatcgga agtcacagaa ctgacaatta tagctcgtta ccatattatc ctcggctccc
84901 ttgctggaga agtccttctt tttagcccca ctcgagctct tgtctgtctt ggtcattgtc
84961 tctccttctg aacttttggc tagatcattc tgattaatga cagagcccct ggaaagctta
85021 tcatttaact ctggattaag gccgcctgac tgcactgctc cgtgctgctt gccataatgt
85081 tctagcagtt taagtgagct agatgactca cagctgaaac tacaaaattt acaccagtag
85141 taactggtgg cctctgtacc attttgtcta gaggaatcga ggggctttat gggcactgag
85201 tacccaacag gagtgtcatc tcctgctttg actgttatct tgtcctgcca ttttcccaag
85261 tctccagaat cactggattg aagtgcaggg atggacttgt tagagttttt ctctgaaggt
85321 tttgcaacct cagaggaggg gagagaagct tttattttgt ttgggtgagt ctgaagaaaa
85381 tgttgttcta attcggtgga tgagttgccc atataagtga aattgcagaa tttacagcgg
85441 aaatacttgg tgttcccttg gcaatctggt gttttccgtc caatgccaat gaatgttcca
85501 cctgaagtca cctggagaac agaagaaatg gactttactt ccaaggtgtc attaattcaa
85561 tgggccctct gcaggggagg gggagggtac tgccaagtat tccacatcaa aaaatacaat
85621 agtactcctg cttattatga aatcacttga ataatcagag ccaaataaat tcactcttca
85681 tttctcaaca gctactcttt gagaggctca ttatgttata cactgtaggc caaagtaaca
85741 ggtgaaggta agtaataaat gcactataat ttgaaagact taataataca ttttttaaac
85801 taagaaaaca attttcaaca cagtacattt tactattgcc tccttcaaat cacattccta
85861 cagaagcatc caaatcacat gggatttctt ttcaaaaggt gaagcgttac tgtcaaaact
85921 agaggcactt gctcaaaaat tactcaatgc agatgttctc caaggaaata ttctgttcag
85981 ttcctacagc aatgaggctg caggcaaaga tgaggtctct gttttaggta aaataaaggt
86041 gctccaatga ccacccaaaa tggcttataa ttaaagcaca gtgaaacaaa caaaaagatg
86101 aattgagtat gcaggaaata ctagcaaaga agaaccgtgt aaataaagaa ttttgaatgt
86161 ttccccatct agaacaaaca aaaaaaaaga aaaatcttcc atgccttatt tgattaaggt
86221 tctgtttcat tgcaaagggc tcccaacttc tcacatgagt aggtcatgat gtctgaatgg
86281 taaaataaaa taattccacc ataaaaaatc actacaactc tgctttcttt cccaaattat
86341 gaaattacat ggaatcagga ctatgaaagc cttcaaaggg cactttgcat aaaatccttc
86401 ctccattctc agtgtgcacc tcaataggca gtacaattaa gccaccactg agcagccaaa
86461 attcagagtt aaaatagagt tttttgaatg aaatactcca aaaagggaaa acaatttctc
86521 ttccaaccac cagggaattt ttaaattttc ccagcaacca gatgtaatat attaagcaag
86581 tggcataatc atcttctgcc ttcttcaaca gctgttcaaa aatgacacgt gtgatcgctg
86641 ttcatatcca aatcactgca tgagaaaagt ctcaggcatc tcaaaatcac gtaaaggctc
86701 tatttgaaat gcaaataata ttgctgtagt acactgagag atatgtttgt acctctattg
86761 tacaggacta agattgaagt attctttttg ctattaaaaa gagatgtgac cttgatcaaa
86821 ttacttaatc tctctggcca atagcttctt caactggggc aacctccaaa tttatgtcca
86881 gtattatagt tctaagaaat gtgcattaat atataaacaa ctcaacattt actgtacatt
86941 aagataggat cttacagaag ttttccacag cctttaatca aaggatgtaa tatttcacat
87001 taggcaatgt ctataaccag aaccccaatc acatagattt cagtctgtca atgacagagc
87061 atcaaccagc cagaaagcta agaatggggc acccttccag gcagcaacct gacaatcatt
87121 caatggtgtg agaggtttca aagccataca ctcaaggata cagcacagaa cactctaatc
87181 tagcatttaa gtgactaaag caactaaaac ttgcactgaa atataattca taatactgaa
87241 atataattca taatattgta aaaatctcta catatatttc ccaattcact ggaggtaaag
87301 agaaggttca tttgccaaaa aaaaaaaaaa aaaaaaatac atatatatat ttaaatggat
87361 agccatcaat caggttcttt gttccggaac ttaaacttga ttcaaaaggg ataaattcag
87421 atgtatcccc ttctttaaca caggcaaaga aagaaagtat gcaaaatcta gagataaaaa
87481 ttttcttgtt taagctttca cattgcatct gccttgccaa gcttccctat tataaaatgc 87541 tttgaacttY tgcttgttga cccaagattc ccaagaaacc accaggcatc atcactattt 87601 ctttgatttt gcaatcttta agcctcatgt gccatgacca cactgaaagg gttgaacaaa 87661 gagcatacat atacttaaga aatccctaaa catatgattc tgaagtggat ttaaattatt 87721 actaagcatc ccaacatact aaaaaggttg ttgttattta aaaacaacct aagaagtcag 87781 aaaacccagg ctacgaatca atactatcag gagaagggac tacgaaagaa ggcaactttg 87841 tgaaaaatta tcagtaaagc attttggttt aggattaact aagctataaa aaacgagtta 87901 ggtctttgaa gttggtgtac gtgtctagat gtgttttttt ttttttgtta aacccaggga 87961 aaatgtgcca acttcattat ttaagaagct tttaagggct atatatttta attatgctga 88021 agaatgaata ccttcatact attgactgct ctttcaaaag ccaagctaaa aaatgaaaac 88081 tcacggatct catttaatgg caagaacaat aatgacactt ctataggtca catatttgcg 88141 tttttggtta atatcggtaa ataatattag tattatatat tattaaaagc taatataata 88201 ttagaatcac atattttaaa acgcagtttt ggaaaacata atgacagtgt tctattcctt 88261 ttttatttca aaaagaacac ctaaaagctc tatttgtggc aatttttata ctttctatac 88321 tggtagatgt acactcaagt catagcgaaa tatagatgac atacaacatt taaatgccat 88381 tctacaataa aacataattt attatt'ttag gagacactac tggtgtctat tgaccatact 88441 cattttattt gcatgcccca tttatgctat tttaaatcta ttcttttcta gggtaaaaac 88501 aaatacacag tgagaacaat tatgcaaaat aagtgttttc agcaatggtt tattgaaact 88561 tattgtctgg aaacagtgcc tacacRttca tagaatattg caaacttttc aaatggctga 88621 atccatcacg gagtaggaaa atttactgct tcttagctgt gactatacta aaaagagagt 88681 gcaagcagaa tagagagcag agctttcaaa cttccttctt ttactaacca gaactttaat 88741 tataaccctt taaacccagc agttcctttg gtaaagacat ccaaattttt acaattatat 88801 taaactgtaa tttctgtttg ggccaaaaac tacacgtatt tatccctcta attagtagta 88861 actaagtgtt cggcttgtgt cagactctgc aatagatatt aggcatataa tattgaagag 88921 gtagacaaca tctctgcctt caagatactt acattgtaag atttaaaaat tgtagataaa 88981 accctttatt tatttgtatt tacatctgcc tgtatttatt tgcttctaga ggaagttctc 89041 tagctcaagt tctatgaaaa gatacattat ttaacctctt ggaaccttag tctcctggcc 89101 tctatggata caatactgta caacttgtca ttttatagga ttatcgtgag gatcaagtga 89161 gaactatgga tttgtgctgt ccaatagtac ttcctgagat gataaaaatg ttccatagcc 89221 ttccttggcc aatatggtag ccactagcca caagtaggct tagtaaaaat gaagaactgt 89281 tttttaaatt tactttcata tatttttctc agcatttcac taatttattt gtaattaact 89341 gaaatgtatt tattttaaaa atttcttttt tagaggcagg gtctcagtct gtcacccagg 89401 ctggagtgca atgtcacgat ctccgctcac tgtcacctct gcctcctggg ctcaagtgaa 89461 tcttcccacc ttagcctcca gagtagctga gacaacagac acatgccacc acacccagct 89521 aatttttgta ttttttgtgg aaacagggtt ttgccatgtt gcccagactg gtcttgaact 89581 cctgggctca agctagccac ttgccttggc ctcacaaaat gctgggatta cggcatgcac 89641 caccatgccc agcctaaaat ttaaatagcc acattttgtt agggaatacc atattagata 89701 gcacactcaa cagtaacatg tgcataggta ggattattat ttaataaaat gaatcattca 89761 agtaaatttt tcctgcaaat gggataaaga gattacatag aagggaagtg gtaaattcat 89821 tcagtgacaa actcacacac ttgtcactat caatattctt agctgtaaat gcaaaggttc 89881 aatctgctgt tactaagata atacttctgt ttatattcct tcttgagcta caagaatagt 89341 cttattcaca tctagaagaa agcaggagtt caatgcattt tgactgaatg aatgaacgaa 90001 gggagatgta agcagatgaa aatgtctcac caaggcccct gagttacctg agattcgctg 90061 gccacagtac ttcatggcac cctcaccact gacttgacct ggcatacatg gccttcttca 90121 agaaactaca aaatgcatct tttgtccaca agcaaatgac tgactatgga gtcctttgat 90181 tgtgaattaa agttatataa aaacacctaa aacaattaag gcacaaaaaa gagtatttta 90241 aagtcttttt cactgactaa ggactagcat ggctttttat acttgatttt agccctagtt 90301 ttaaaaacta ttccttccat gaagtgtttt ccatggcttt catttataat ttatttacat 90361 atgtcttttt acacacacac aaaaatgttt taccgtagaa gtgaagaata taaaagggtt 90421 ggtttctttc aaggtcatta atctcagttt ctttctttcc tcccaataat atggagcaaa 90481 agctaacaag aataatatct aaaataatat gtcaaaggaa aatatttctt aaagcctcag 90541 agctcttaaa atgacacttc aacacatgta cttattttca acgcagcaaa gaaagagttg 90601 tttgaactaa acacaaacct cttctcagtt gtggttcatt ttcaagtact ataattgata 90661 gcaacagaga caattataag ggtactaatt taagagaact tgaggttatg ggctgatagt 90721 caaagaggta aaagcttaaa aataaaaagc taagttgtta aaaaaaaatc actgttcaaa 90781 tactgatatt tcgaataact tgaaaaaagt ctgtaagggc cgatcttagc ggaaaataac 90841 tacagccaga attaatctgc ttatatacat cgggaatttt tctgcatgta tagtgcttca 90901 aaaaaacatt ctagaatata gagtgtgctt cagtgatata cttttgatgg agaataaatg 90961 tttcagaatc tcaataacaa ctcaaattat atggcatcat ctgactctca tgccatccat 91021 agcttatttc tgaggtttat tcctatcagt gacactgtaa caactccctt atttggattt 91081 ataatacaag agccaaccaa cggcagggga gggtacaggg gaaaccaaaa ctcaaactcc 91141 tacactgggc acattgaaga agaaaatact gcatattcct cattatgMag atttttatta 91201 ttggcttttt gcataatagc aataaacagt ttaccctaaa ctggttttct cattttactc 91261 agtataattc tgaattaatt tttctatatt atctggtatt tcagatgaca gagtaccaat 91321 aaatcttaaa gtccatctta ataggccggg gacagtggct cacacctgta atcccagcac 91381 tttgggaggc caaggcgggc ggatcacctg agatcaggag ttcgagacca gcctggccaa 91441 catggtgaaa ccctgtgtct aataaaaata caaaaattag ccagatgtgg tggcaggtgc 91501 ctgtaatccc agctactcgg gaggctgagg tgggagaatc gcttgagcct ggggggcgga
91561 ggttgcagtg agccgagacc atgccactgc actccagcct gggcaacaga gcaagactcc
91621 atatcaaaaa aaaaaaaaaa aagtccatct taataaattg gaagacaata ttttatagga
91681 Raaataaaca agacaaaatg cagttcaagt gggttacaaa gctaagtgaa ttccccatag
91741 cactcctcta cagcatccaa cctgcaggtg ctgctggttc ataatggaga tatgtcagaa
91801 ttgcacatgt attctaaaga gcatcttaat gggaaaagag aaaatctaat ctccgaaaat
91861 atagaacatc cttcctccag ggaaggttgc tgttttaaca catctactat gtgccaagca
91921 ctggaaatac aaaaagaatg acatagtcaa aaatcgtaac atgtctatgg tttagcacaa
91981 aaagctcaca actaaaaaga caacaacaat ggaaaacatg cttctgctga gacatgtaac
92041 aggttccatg acaacacaga ggaagagccc ctaacccaga ctgaggagtc agcaaaggct
92101 cccagaagta acaactgagc tgtggacaga tgagctgata tgtgaaggtt agcctcaaag
92161 gcagtgatca tgaaagggca tttcaggtga aggaaacaaa accagtaaaa tcaacagcga
92221 gagtaagaca atgcaagctg ctctcaggaa atactgagga gggccgaggc attatcaaat
92281 cgttaaatgg aatatggtac ataacggtgc ttgctatgta ataagtaata taaatgtaat
92341 aagtaatata aatgtgctag ccttgttctt actattatcc tttctggaag aatagtgaga
92401 tttaatctgg aaagttaatg tcttattgga gagctgatat ttacataagc aagtataatt
92461 agatgatatg cttttcaaca gaagtagtcc catacttgct ataaaatgta ggacaactga /
92521 tcaataaaca gaatttttaa aaattaaagg aaagaagaaa gaaaggaagg tgagagggtg
92581 ggagaaaaaa agagaaagga aggaggatga aaaaaagaga aggagaaagg aagataaaag
92641 aaggaggaca gaagaaaaga acagaaaaag aggcMaaaga gaggaggaag aagaaggaat
92701 gaggaaggaa ataaaaacag aagaagacaa gaaagagcac aaaaaagaaa tcaaaggaaa
92761 ggagaggaag atgaggagga ggaggaaaga ataggaggtg agaagagaag ggaaaggaag
92821 agacaagaag ggaagataaa gctagaacaa cagaaaagaa atcaataagg atttacttga
92881 aagaagtgac agtaaaagaa taaagaaggg aacaatggga cacactgctc aagtgtttgg
92941 ggcgtgatat caaacagaaa tccaaggtaa ctccaagatt tccagcctga cttagctgaa
93001 aacaggagta aagaagaaag gaggcaaaaa gaaagttgtt gtggggaact tcacttttac
93061 atatgttgac tttgaagtgc caatgaattc caaggtggag cttcccatca gctggaaaaa
93121 caggtcttgg aacaaaccat catttattat aagattccca Ratatgttcc tggacacatg
93181 aaaggatgca ggcattcttt aatcttatat caacagttca actaaacatg aaaggagaaa
93241 tgctaggcca ggagactctg tgaattccSa aactcctcac tatgtcccac ccgtctattg
93301 ctttctgaat tcaatgaaat cacacagctc caagcacagc tggagaatcc agcacactca
93361 aattggacac tctaaaggca ttctgatttg ttctctgctt ccacacccat tcctgcactg
93421 aatgtaggcg gaggataaaa ccttaattac acagccttgg aggacataaa tgggaatgga
93481 gtgtatctcc ttaacatgta atctgcatgg aaaaaaaaca acctaaattg tgcctggtaa
93541 ctagaagagc ataagacaac tttcagacca tcatttaaat aataaattcc taaaacaaag
93601 tcctctaaat gaggggagta caaaaaaagt caaacacctg gatgaaatgt cccttaattt
93661 ggggatcaaa cattacatcc ttaattgatt acatcaaaat ttgcacctta ttcgctctac
93721 cagaactaat tcctgcagca aaagtaccag ggaaaaggaa tcactgtaat tttaaaaaga
93781 tgagtcagca aatttaattt tctttttact gatttcgaat agaagcttag taagttcaaa
93841 taaggtagca ttacactaca cggagcagat agcagataat ctcaaagaaa tcacccaagt
93901 ccccaaaatc aacctcattc agagagactg catcaaagag ctctgattta tgtcttctat
93961 ctcctccctt acatgcaatt ggcctaccct ctattgcttt gctattggca gtaaactcag
94021 actcatgttt ggccaggcca tatatcagtg tggttgtgaa gacaggcttc cgtggcctgt
94081 ctatcatggt ttacatccca ttctctctac ttatgcaact ctgtgatctt gggcaggata
94141 ctttaatata tgctgtttcc ttattttttt aaatggacat aaggttagtg tttacttcta
94201 gggctattat gaagatgagt tcattaatac atgtaaaaaa ctgagctcca catacacaca
94261 taagaaccct tagtaaattt tagctattat tagatgtgaa tccaaaattg gaaaaaaata
94321 agggatctgt acaaattttg attatgtaaa ctaaaagatg aaagaagtat gaatcaactt
94381 aaatgtaaga cagaaaaata aattttacat ttgactttca attaaaatta aacaagggtt
94441 tgtatgatat tctgaaaacg tataacattt gcctcaaatg tggatcgcct gatgaagaat
94501 tcaagacatc tatggtctaa tgactgtcag aatcagactc tacaaaggaa atcagaaaat
94561 gaaacagaac aactttctct ctccaaactt tccccgtatt ggcacactac ttgtatatct
94621 gtgtttctaa cagctgcacg tatgctgtaa ttacaatgat ccaagaaata aaaaaacact
94681 gagtaacctc ctgacttgag atggttagca gagcaggcca taggagaggt aaaagatttt
94741 cttaggagtt gaataatgct aaatttgatt gaatccactg ataaccacaa tttattctcc
94801 taaacattct gcatagagaa aaatactgat attcaacaaa attatacatg ttcctttaaa
94861 tgataacaaa taaaccaaaa atcaaagatg ataccaactg tcatgaacaa ggccaaaaaa
94921 gatttaactg tcatgcaggt aaagggatta gtaatgatag ttgaactatt ctactagggt
94981 ggctacatta tttagctctg ccatctccac tgatgaaaat aacctggtaa ataccagaac
95041 tttgccttgc tttatggcct tgaggtgagc atatgtctta ttttgcacac tttattactt
95101 gttaaggaga cacagtcctg aaaaagaagt gtgggttcat atgccaaaca ttctgtattc
95161 tatagacagt ctgaattgat aaaagtctca ttcaccagtt tttctaaact gcaattgtcc
95221 tcaactcaga aaaacaaaga aacagaacct gctaagatct acaacttccc tcctactcca
95281 gatcaaagaa aatgccaagg caaaaaataa atgtttagaa gtaaaattca agacaagtca
95341 aaataaacca cagtaagatt aatggtctct gatttttttt tcaaaccaaa ctgtaatccc
95401 ctgctcaaac gattgtactg atacatgtta aagatctata aactcataca attaaaYctg 95461 ttgaaaatac aaatccaaag gatgacttcc tctctctaaa atctgttctt cctgcttcta 95521 acatgacata ttgtgcaact ctgataaaaa tatttacaac acagaaatgt aacactccta 95581 gctcaaacat aagacagtac ttggtaagtt aatcatgtga ttcttctatt agccttagaa 95641 tttcattaag ggccaaaata aatttaatag ctggaaacca gtagctacct tcttaaagtc 95701 ctgaataatg cagtaataaa aagaattcac taatatgaaa aggcaacata tatatcataa 95761 gcatctgaac ttgggatagc ataaacactt tcccgtggga agcacaactt gacaagactc 95821 ctacttttat aacctgatct gagggctaaa agctccccaa taaagatgat gcaaacaccc 95881 aatgcacagc gcattacacc ctaatgaaac agcaaaggcc gcgttgttaa tgcaggcctc 95941 cattcagtaa tggtttaaat ataagcataa taaagcagat taaaaagaaa gaagctagca 96001 gggcgcggtg gctcacgcct gtaatcccag cactttggga ggccgaggcg ggtggtcagg 96061 agttcgagac cagcctggcc aatatggtga aaccccgtct ctactaaaaa tacaaaaaaa 96121 ttagctgggc gtggtggtgc acacctgtta tctcagctac tcgggaggct gaggcaggag 96181 aataacttga acctgggagg cggaggttgc agtgagctga gaccatgcca ctgcactcca 96241 gcctgggtga cagagtgaga tctccatctc caaaaagaaa aagaaaaaaa taaaaagaaa 96301 gaagctgtaa ttactttttt aagaactcga tctcttgtat tattggtctc catgtgagcc 96361 tctatggccg aatacagata gtaaatatta catgaaaagc taaccaatca tgctgcatct 96421 atgattttta caaatttaag catgacaact gatagaacat gctggataaa tgaaaaattg 96481 gaaaactaac atcctggtca aatcaattag ctctaagcta gccattatag cttctcttgt 96541 tttcttggta aagaatttac atgtcttctg cttttaggac tgggcatgct acctcaaggt 96601 gttgggggga gaatctgata aatgccacac tcagaaagga cagtcttggc tttctgatgg 96661 ctatttacag tgacctccat tatccggctg gggctctatc ttctatgcag cacaaagaaa 96721 cattgttatt tggcagtatt tttacaaagt tgtaattctt tgacttagtc atttgtgatc 96781 taatttatac aaaagtggat attatcagca caagcatgtc taaatttcaa ttattcctcc 96841 aagtatattt gaaataactt gcacttaaac cgggaaatct tcgaatttgt ttaaattatg 96901 tacttctttt cccaactgta tatctcatat ttgctctcaa gtccattcaa attatttaaa 96961 accatccatg tcaaaccaaa atatagatca aagccataga gttctttcag tgttttgctt 97021 ttggcttctg ttactttttt tttttttttg gcctaaaatg tcataaatct atcattaacc 97081 accaactgtt ctgaatgttt tttaaaaagc aagtttaatt gaaaaggatt aatgaattct 97141 tcatctatta cttcaattag cttaaattat ggacttatat ataattccta aaattgaacg 97201 attgtctgta atactcatat ttaccaacat ctttttatca gaatacacac aggccattaa 97261 cttactatat atttctgaca aaactacatt aaagaaattc tatagtcagc attttatctc 97321 ccacagcttt tagcctccac gaagtttaac caccaaggaa agaatctctc aaatagcatt 97381 tcagtaacat aaacttgttg gtataaaagg aaatttcaga tgcgttttta ttcattttat 97441 gcaKatagaa aaactgttca atatttattt cagcatgaag atactaataa gttacttatc 97501 ttcagacatt tttctgcagc agtatctggc atatgtgcaa agcattcaag attagcattc 97561 gatctacaag tatattcagt gttatcctca caaattgaag ttaacccttt agatgcgttt 97621 caaaatccaa ttgtttgttt gaaataatca cgcgctgttg ccacagatta cttttttcta 97681 tttttaacag tattatttat cttggaaaaa aattatctta acaagattat gttcctgcat 97741 gctctttcct accacttttt gtcttcctcg gtgtgtgctg caaggattta gcagacagca 97801 tagtctagga tcagctgttt tatttctagt tctattagtt caaactcaga tagctcacac 97861 atctgtttta cttctaaaat cactgcccag gaaaagtgtc cagctccttc tacccaaaca 97921 taaactttac tataaataac taagaaccta acaaataact gtttggggga aatggaagtg 97981 tgtttaaaaa tctgaattct tccaaactat aggccttagt aattttcgtt tttagagttt 98041 tccctgacag cctagatcag cttccctcag aagttgtccc tagagtgaac agactgaaac 98101 gcctgccgga aatccaggta ttaacttcat attacttgct aattataaaa cactgtttca 98161 catactgaca ttattcactt ccaacccttt tatctaaaac aagtatttgc ttcccaatca 98221 tagtttgaca gcagagtgta tctgacataa gacagtgaaa tgatctttaa tagacagcag 98281 ttcaaagcat gtagctttca tgcataataa ctatgttggg ttaatttgct gctccacaga 98341 aataattcta tctgtggaaa Wtcacaaact aagatggaat ttttagagaa ctttaccaag 98401 atgaaactgg tgttttaaga gttctttata tctcccttaa agtgataaga ttgccattct 98461 gacatttaaa ggaccaggaa atttctcttc tgattcttct tacaagtatt gcaaaatatg 98521 ttcactacct gttatttaca acctctttgc ctgcttttct taattggtat taggtcaata 98581 gagaggtctg agRcaaatga gctgtagcta tcaagtggat aaaaatgact gatattttaa 98641 gggatttatt tcattagttc tctgggtact tctaatcatt cctaaaaaaa gttactagta 98701 aagctaatat gttttgaaat ccccctggcc ttataataca ataaaaatct acaaactgta 98761 acaaaaacta atacactttc tcaagctgac catgaatttt
[0379] Following is a genomic nucleotide sequence of a PRAME region (SEQ ID NO: 5).
>22:21166551-21263900
1 tggtgctact gaataatgta tggtttgtat tttttgtttt atgaggtttt ttaatttggt
61 ttgaaatact tgctttagat ttactgaaac tagaattaat gggacttttt tgaaattttg
121 cttttagacc tggggagtga tggttcagag tcagatgtgc tcacagttat ggatgctaca
181 tccactgatc ctggctgaag aggttccagY gacacttgaa tagtaacttt tgtttcagga 241 ggtaatcctt ctagttgctt aggcttctta aacatttgat gacactgggt cttgtgctcc
301 attttctcct tgaaagttaa aaactgtagc cggcacttgg aacactggtg tgcactcttt
361 ccccagtggc ccctataatg acacatgtat ggtgttgctg ttttgaaaat tttgagacaa
421 aagggacaaa gcaaattctt tgtgttttca tggcacgttc taaaatgtgt ttctacatca
481 gcaaagaccg acgatctata atggcaaacc tggcacacRt aRggcatttc gccaggctta
541 tgatggtcct tcatgtgttg taaga.ggacc tgatctgttt caaatgacaa ttcacagatt
601 ttacagacag tagagggctc ctgggcagtg tggacatttt cgatgtgaca ctgtagctgg
661 aagggagtgg gaaactgccg gtggcagtgc tggcaggtgg tgtggttttc ccagctgtcg
721 ttcctctgct tctcaaattc caaatgatgc ttcacgtgat tcataaactt aacatttttt
781 agaactttca cgcagctgag gcatttaaag gtggtgtgag tcttctgttc cggctgccct
841 tctcctttat gctgtccata gtaaaagtca ctaagtaaca caatgggatt ttctttcttg
901 ggatcaaagg tcttgttttg acttgttaga ctcaaaatgt ctgtttttgc caattcattt
961 Kccctatcaa gatttgtatt tataggcttg aaatggatat tgtcctttgg aaaagctgct
1021 ggaaaaggtg ctccattctg aacatggctt aatgaggtat gaacattatt tgagggtgta
1081 ctttgctgag tattcattgt atgaaaggta tctgaaggga aYgaagctga agaatttcct
1141 tctataattc catccctgag tttagccctt ttgggattta tgctgtttac ttcgaaagtg
1201 gaaagttgct ttgatacacg aggactttca tttatacctc ctactgaaag tgctgtactt
1261 actggatgat gcaatgaatc tgtgaatgta atcaaaggag aaggtaattc tgaagagtta
1321 ttaggcacaa cttgtggtga actatttcta taatcaggtt tagacaaagg ctcaataata
1381 ataggactat ctgttgatct cgattcaagt tgggaagctg gcaggacggt cactgcttct
1441 gatgtaacgg tctcatgact tttaggctgc aatttgcgag cagtatcttt tctaaggtga
1501 tcatactttt ttctccttga ccatgaacoc ggggtgactc tgttcaaaat gtttgaaacg
1561 actggttttg aatttgaagt caccccaaca aagattagct cagcatcttc atttacatgt
1621 tccacaccaa caaagatgag ctcagcatct tcgtcatcta cttgtttggt ttcttgtatg
1681 ttcttctgtg gttcaggctc tttctcttcc tcacatgatt gttccatttt ctaatttttt
1741 tattcctgaa tggtggggcc acaagtccca atgcttcctt tatataaact gccacctaag
1801 tacaaacata catcagtaag tacaggaaaa tgcattacct tatttaattt tcctccaaaa
1861 cattgttttt ataaaccact attttaccca caaggacact gagactgaaa gaggataaat
1921 aacagcttaa aacctgtgat aaaagatcag gttcctatga atctagaatc ataactttgc
1981 tgtactgatt gtgctctatt ccattttaaa aaaaaaacct accagcagct aatcactagg
2041 caaaattata tggaaaagac acacattttt atgagggcat cattgtgcag tgtttagaaa
2101 catgggcttt ggagttgaag agtaagctgg aatccttagt ctgccacaca ccagatgctt
2161 cctctttagc aagtttttta cttttaaatg tattttgcta tcttaaaggc aaaaattctt
2221 atttcttaga cttacagtaa tagctggttt tttaaaagca ccacctattc aatggcagct
2281 tgatttgtag tggaattgga agaaaattac aataaatgaa aatatctcaa ttgattcata
2341 tatagtaatc tcataaacat taatatagaa aagaggtatt aaatttatag agttagagta
2401 cctacacata accaacaaac agcatagcac aatgtcaatt gccaggcaga taatctcatc
2461 ccaactcatg gccttaatta taatccattc agtaatgcct cctaaatttt cccctccagt
2521 tctgacttac ccactgaact taaacacaac agtgcttcct cctcaatctc ttctccacat
2581 agcaagcaga ttgattgtgt taaacaaagt cagactgtat cattgttcag ctcaaaaccc
2641 tccaacggct ttctgattaa ctgagagtaa aagaccaagt attaccaatg cctagaagac
2701 tacactacca accccacata ctgctcctgc agttatctca tactactctc cactatccac
2761 ttcactgtag tcacactaac ctccttgcta tttattttaa ttttttaaat taaattttat
2821 ttttttgaga tggagtcccg ctctatcacc caggtatata tttcagctca ctgcaacctc
2881 caccttccag gttcaagtga ttcttgtgcc tcagcctcct gagaagctgg gactacaggc
2941 atgcaccacc acacccagct aatttttgta ttttcagtag agatcgggtt tcaccatgtt
3001 gaccaggctg gtcttgaatt ccttatctca ggtgatccac gtgccttggc ctcccaaagt
3061 gctaggatta caggggtgag gaactgcacc tggccctccc tgctatttct tgaacatact
3121 aagctctttt ccttgctcaa agcccttgtt ccttccaata tgaatgatca tttgggtatc
3181 cgcatggcta gctcctcact gcccttaggg ctctattcca agtatccctc tcggtgaggt
3241 tcttaaatat tccaaccaac ctctaaccca cattcctaat ctcttttccc ttttctttca
3301 tccccaaagc cctatcacca tttaacatat tacgatccta tttatcttga ttattttcta
3361 ttgtccatcc agtggaacat aaaactcaaa agggcaggta ttctatcttt tgttgactgt
3421 tgtatgccca gcacaaagaa cagtgtctgg cacaggacag gtgctcaaca aatacagaat
3481 ttgttaaatg aatcaataaa tatcgactct ccttcataag atcctttatt tttaaagaag
3541 attatgctcc attaattcca cattttctat gaatttactc tgagaacttt atccattgga
3601 aattccgtag gccaataaag gacttaaagc tagcttttta caaatatttg accacaacca
3661 atagtttaaa aaaaaaaaag tgatatggag tccaattaca caagcacata cacataaata
3721 ataactacct ttactacaca ggctgcattc taaaattttc tatttcattt ttcttgtctt
3781 tttctttttt tttttttttt gagacggagt ctagctctgt cgcccaggct ggaatacagt
3841 ggcgcaatct cagctcacgg caacctctgc ctcccgggtt caagcaagtc tcctgactca
3901 gcctccaggg tagctgggat tacaggcaca tgccaccaca cccagctaat ttttgtattt
3961 tttagtagag acggagtttc actgtgttgg ccaggctggt cttgaactcc tgaccttgtg
4021 attcaccagc ctcggcctcc caaagtgctg ggattacaag catgagccat ggattacaag
4081 cctgagctgt gcccagcctc atttttctta aactaaccac agacccacaa aatcagttaa
4141 gcctcatcac taggtcacta ctagttgttt caaaaactgg tctaatgcta gcaaaatgta 4201 cgcactagaa accaaggttc ataactcacc aatttctaaa aacatatcaa gattcactgt 4261 atccccatgt ggacccttca aatcagcata atccaaagca taaaaattaa attgacatct 4321 aataaatgcc atgcacattt gttttaattt taattaggat atcagtatct aattttggaa 4381 catatagaca atatttattc ctataaatat gtgtatcacc ttggaagtga atttaaaaag 4441 gaatacaata ttaagtaagc attgtcaact atatacaaac tggtgtatca tgaatagtga 4501 tttacaactt aattagtaga tatataatgc cagaaaaaat ataaggcaga tatgaataca 4561 ctgctctctg gagtcaacta aaattggttt agtctgtcta tatacaactt caatcgccta 4621 actccaggat gatgtcacct agcccagtgt ttcctaagca ttactgacat ttgggactag 4681 ataattcttt gctgggggct gtcgagagca ctgtaggatg tttagcagca tctgtgatct 4741 ctacccacta gaaaccagca gtagtccccc agggtggcaa ctaaaaatgg ctccaggaat 4801 taccaaatgt cctgtgggaa aactcctgcc caaatccaat cttctcagga tcacttccac 4861 atttccagta actaccttct acataattct aaatgtattt tctaacatcc tctctaaaga 4921 aaatatctgg ccgggcacgg tggctcatgc ctgtaatcYc agcactttgg gaggccaagg 4981 cagttggatc acaaggttag gaattggaga ccagcctggc caacatagtg aaaccctgtc 5041 tctactaaaa atacaaaaaa ttagctgggc ctggtggtgg acacctgtaa tcccagctac 5101 ttgggaggct gaggcaggag aatcgcttga atccaggagg cggaggttgc agtaagccaa 5161 gaccacgtca Ytgcactcca gYccgggtga cagtgcgaga ctgcatctca aaaaaaaaaa 5221 agaaaatatt ctaattcaca atcattaata ttctccaata tcctaaaaaa tcagactata 5281 atctgacaac atggccatta gatttatctg aagattaatt agtttattat tgttattatt 5341 ttttcttttt caagacggag tcttgctttg tcacccaggc tgaagtgcag tggcatgatc 5401 tcggctcact gcaacctctg cttcccaggt tcaagttatt ctcctgcctc agcctcccgt 5461 gtagctggga ttacaggtgc gccaccacgc ccagctaatt tttgtatttt tagtagagac 5521 ggggtttcac catgatggcc aggatggtct tcatctcctg acctcatggt ctgcctgcct 5581 tggcctccca aagtgctggg attacaggcg taagccacca tgcccggcca atctgaagat 5641 taatttttaa agcccccaga agtttatgat ttactaaagt tatatggagg gtttgtagac 5701 agagttagca ctaagatgcc taattcctga attctggttt tgtgctcttt ctgctaaaac 5761 tgacttagtg cacacttatt tctgatgata ttgctgttaa ctgcttagtc tttaagaatt 5821 aaagtgtctc attggattca ttttttcact ctctattttg taaacctgtt caaacctcct 5881 ctacctccca gaaaatgtca aagtacctta taagccattt taaataactt ttagagcatc 5941 actcattcta cctctcatgt ccattcctat taatggcccc cagcccaggc cgcaacttct 6001 aggtttaggt ccactggtta cttcattttt gtacacttgt actagaccct aaattgttct 6061 gtctcttttc tctgtccttc aaattatata acacaacacc acaagatgga tcttaaaaca 6121 caactgtaat tatatcactc ttctatccct aagcgttaaa tgattcctcc aattctagag 6181 aatattttag gtatacataa tttgtgttat gctatgggct gaactgtgtt ccccccaccc 6241 ccaaatttgt atactgaagc tataaccccc aatgtgatgg tatatagaga tggtggcttt 6301 gggtaataat caggttaaga tgtattcatg aggatagggc cctcatgatg tgattggcgc 6361 ccttataaga ggagatatca cagaccttgc ttcctctttc tgtcatgtga gaacacagca 6421 agaaggctgt catccaccag ccaggaagag agccttcact aaggaaccaa atcgtccagc 6481 atcttgatct tgaacttccc agcctaaaaa tgtgagaaat gaattcctgt tRt.taataaa 6541 ccatcaaaca agtaaagaag aaaaacttaa actgggttct aatgacactg ctggacttcc 6601 taaccagttg ctcttggtta gatttttggc tgttgtccat actgattaac cctccatcca 6661 accatcaatc aaggtgaaaa agaggaatag agcaaggatc aggaacaaag ctaataagtt 6721 aatgaacaag ttcattaaga acaagagtag cattagacca tgagagtttt cttctcctct 6781 agatgtccag agacccttct ctacaaatgt ccaccaatgg atgaatgaat gcttattttc 6841 caatttgcca gaatcttgag atactggggt agggaggtta gacatgcaaa gatatatcag 6901 atctaacagg tttgtcctca aaaatcaatt ctcacctccc atgatgggag aagccaatag 6961 gagggcttca attccaaaga aagctgagag atcagtggat gataacccac cttccagatt 7021 atttctatgc attcaaacaa tggcccttct actagatcat tgttttttgt tttttttgag 7081 atggagtctt gctctgtcac ccaggctgga atgcagtggc gcaatctcgg cccactgcaa 7141 tctctgcctc ccgggttcaa gcagttttcc tgcctgagga gctgggatta caggtgcgtg 7201 ccaccacgcc tggctaattt ttgtattttt agtagagatg gggtttcacc atgttggcca 7261 ggctggtctc aaactcctga ccttgtgatc tgcctgcgtc ggcctcccaa agtgctggga 7321 ttacaggcgt gagccaccgt gcccagcctc tagtaaacca ttgttatWac cacacacgag 7381 tttttaacca cacacatact cttctgagga aaggcttccc caaactattg gtccttatga 7441 tgagatctgg ccatatatat ttttcttctg aaccccagga ttgtggggct gtcacatttc 7501 ctctaacagt acaccttagg agctcccaga cttgaagaaa attggctgtt tcccacattt 7561 gaacattttc cactttttcc ctcagtctac ctttttgaat tgatttctga ctacttctag 7621 ataacctttt tccattaatg aatattgagt gcttattgtg tgccaggcac tgctttaggc 7681 acttgagaga aatcggtaaa cattaacaga cagaaatgaa caacaaaaac aaactatttc 7741 aacagaagga actaacaaac agtccagtgg aggaagaaag atagtaagta taataaaaat 7801 gcaaattctc tggtgaactg gaagatgacg ttatagaatt aggaaaaaaa taatagagca 7861 ggcttagaag ttgaggaggt ataagagaat tgtaatttta cgtagtgtag tcagagtaag 7921 tctcatggag aaggtaaatt tggacataca cttgaagggg ataaaaggtc agagaggtag 7981 caagagtgga aatagtagta aggatctgta aattcttcat ttttctctca gtttagtctg 8041 tggcaactgt catgtctttt gataaggaac gaccttggtt taatagttac tcccctccta 8101 gactctcagg ctagattttt ttttttttta attgagacgg agtcttgctc ttgttgccca 8161 ggctggagtg cagtggcaca gtcttgtgca cagtcttggc tcactgcaaa ctctgcctcc
8221 caggttcaat tgattctctt gcctcagcct accaagtagc tgggattaca ggcgcccacc
8281 accatgcctg gcgaattttt gtatttttag tagagatgga gtttcaccat gttggccagg
8341 ctggtctcga acccctgacc tcaggttatc cacccacctc ggcctcccaa aatgctgaga
8401 ttacaggcgt gagccactgc gcctggcctt tttttttttt ttttttttaa gatggagtct
8461 cgctgtgtca cccaggatgg agtgcagtgg cacaatctca actcactgca acttctcctg
8521 agatgcttgt gcctcagcct cctgagcagc tgggactata ggtgtgcgcc accatgcctg
8581 gttaattttt tgtattttta ttagagatgg ggtttcccca tgttgtccac actggtctcg
8641 aactcctgac ctcaagtgat ccacccacct tggcctccaa aagtgccggg attacaggcg
8701 tgagccactg cacctggcct ctcaggctag tttcaaccct attttacact cctctaccat
8761 cctcagctta cttttgaaaa agtatttatt aaaaaatatt tatgaaaaag ttatttttgt
8821 tttatttagt tgccctggga tggaatacaa atgtttgctg tagttttaag gatcctataa
8881 aactcccatt tactgcaagg atctctgttt aatgggcatt caattctaag atagcaaacg
8941 tgtagagtta tttgtggggg tgggggacaa gtttgagaaa gaagcagtgg ggtgaagaaa
9001 gtgataggca agggaagatg gtggggatag gtaccatagg agtcctggtg gcctaaaatc
9061 taatcacaga agttactcaa gtgggactga tgcctgaaaa aaatttgtgt gtggctataa
9121 cagtgagtgg gatctactct tttttttttt tttttgagat gggagtctcg ctctgtcacc
9181 caggctggag tgcagtgccg tgatctcggc tcactgcaac ctccacctcc tgggttcaag
9241 caattctcct gtcttggcct cccaagtagc tgggactaca ggcacatgct accacgccca
9301 tctaattttt gtatttttag tagcgacggg gtttgatcat attggtcatg ctgctctcaa
9361 actcctaacc tcaggtgata gacccgcctt aggctcccaa agtactggaa ttacaggcgt
9421 gaggcaccgt gcccagcctg ggatctactc ttaagcaaca gagaaaatca gtcatttttc
9481 cccttttcct tcccttcctc ctatgagcag aatcttggtc tccatcttta aaaaaaaaaa
9541 aaaaaaaaaa aaaaggccag gcacagtggc tcacacctgt aatcccagca ctttgggagg
9601 ccgaggtagg cagataacct gaggtcagga gttcgagacc agcctgacca acatggtgaa
9661 actccatctc tactaaaaac acaaaaattt gccgggagtg gtggtgggcg cctgtaatcc
9721 cagctacttg gtaggctgag gcaagagaat caattgaacc cgggaggcgg aggttacaaa
9781 ggagatttga cagggtggca gttcaaacta aaagtggtct gatagttcag agtcaagttt
9841 cttcactaaa gctgagtttt taaagggctt gaagaatgat gtaaagaggg gttgtggagg
9901 accacaaaaa aagacaacta aaaaaaggca taacaaagtc aacttattaa atgcccagca
9961 acagggtgaa gttaagggga atggtggaat aaagacctac aaatagctgc tgcttcataa
10021 aagcaaagag aacactagca aaaactacaa aaatcacgtt tttcagatcc tggaaattaa
10081 cgaaagcttg taaaactcaa aagattttat tacaaaatgg cttggcggtt cctcaaaatg
10141 ttaaacatag acttatgatc ccacaaatct aagtatacac tcaaagaaat aaaggtactc
10201 aagtacatgt atacatgttt atagcaacaa ctcataataa tcaaaaggtg gaaacaacct
10261 aaatgtccat caatggatga atggataaac aaaccatggt atatacatac aacagaatgt
10321 ttattcagcc ataaaagaaa cgacatactg atacatgcta caacagggta aaccctgaaa
10381 atataccatg taaaagaagt cagtgacaaa agctcatata ttgtatgatt tcatttacag
10441 gaaatatcag aaatagataa atccatagat attaaacaaa gatttgtggt tgccagaaga
10501 taggaaaggg aaaatggtga gaaactgctt aatgggtaaa ggggttttac tttggaatga
10561 tggaaatgta tcagaactag atagaggcag cacacgtaca atattacaaa tgcactaaat
10621 atcactaaac tgtttatttt aaaatgactg atgttatgtg actttcacct caatgaatta
10681 ttttttaaaa ataaagaggg tatattacta ccaaccacac agaaaaaaag agaattataa
10741 aggaatacta tgaaaaatta tctgtcaaca aattagataa cctagatgaa acaaattcct
10801 agaaagacag gaactaatga actgactcaa gaacaaacag aaaatatgaa tggatccaaa
10861 acaagcaata gaattagtaa tcaaaaaact tccccaaata aaagcccaaa atcagatagc
10921 tatgctagta aattcttaac gtttttaaaa gaattaacac caatccttca ttaactcttc
10981 caaaaattag aagaggagag aaaactttgc acatctttct atgaggctag ttattactct
11041 tacaccaaaa tcaaaccaag aaatcatata taaaaaaaga aaactacagg ccaatatcct
11101 tttttttttt tttttttttt ttttttttgg agacggggtc tcgctctgtc gcccaggctg
11161 gagtgcagtg gcgtgatctc ggctcactac aagctccgcc tcccaggttc aggccattct
11221 cctgcctcag cctcccgagt agctgggact acaggagccc gccatcacgc ccagctaatt
11281 tttttgtatt ttttagtgga gacggggttt cactgtttta gccaggatgg tctcaatctc
11341 ctgacctcgt gatctgctcg ccttggcctc ccaaagtgct gggattacag gcgtgagcca
11401 ctgtgcccag ccaatatccc ttacagaggc aaaaatcctc aacaaaatgt tagcaaacaa
11461 aatctaacaa catatataac gattacatac catgaccaag tgggatttat ttaaaaaata
11521 caaggatggt tcattctatg aaaatcaacc aacaataatt ttatttttat tatttttaat
11581 tttaattttt tttttgagtc ggaatttcat tcttgttgcc caggctggag tgaaatggca
11641 cgatcttggc tgaccacaac ctccgcctcc cgggttcaag caattctcct gcctctgcct
11701 cccaagtagg tgggattaca ggcatgcgtc accacgccca gctaatttgt ttgtattttt
11761 agtagagaca tggtttctcc atgctggtca ggctggtctc gaactcccga cctcaggtga
11821 tccgcctgcc ttggcctccc aaagtgctgg gattacaggc gtgagccacc atgcccagcc
11881 tataccattt taataaagga caaaaactac acctgcctgc agaaaaaaca cttgacaaaa
11941 gctaacatcc ttttatgatt tgaaacaaca aaacgattag gaatagaagg aaactttctc
12001 caccagacaa aggcatctgt atgagactca cagctattat agtatcatac ttaatggtga
12061 cactgaaagt acccctgccc ctaagatcag gaacaagaca gatatccact ttaaccactt 12121 caactcaaca ccgtactaga ggttctatag ccaaggtaat taggcaagaa aaggaagcaa
12181 aaagcattca gattggaaag caagaagtaa aaccataatg gagatgacaa atacaaataa
12241 ctcaattttt taaaaggcca aagaacttga atagacattt ctccaaagat gatacacaaa
12301 agaccaagta aatatattca gtgtcattaa tcataaaagc aatgcacatt aaaaccacct
12361 tgagatacca cctcacacct agtaggatga ctactattaa aaaacaaaca ttgttggaaa
12421 gaaattagaa tcctcatgca cagtcactgg attgtaaaat ggtataacta ctatggaaaa
12481 cagtacagaa atgcctcata aaattaaaaa cagaactact atatgttcta gcaatcttac
12541 ttctgggtat atatctaaaa taattgaaag cagggtctcg aagagatact ggcacaccca
12601 tgttcacaga aggactatta aaatagccaa ggggtagaag caacccaaat gtctacccaa
12661 aaatgaataa acaaaatatt atatatatat atgcacacac acacaataga atattattca
12721 acctcaaaaa ggaagaaaat ctgtcacatg ctacatgttt gaaccttgag gatattatgc
12781 caaataaaat aaaccagtca caaaaagaca aatacttttg tatttgtata attccaccta
12841 tatgaggtaa ctaaaatagt caaattcata gaaacagaaa gtagaggccg gaagcagtgg
12901 ctcacacctg taatcccagg actttgggag gctgaggtgg gcagatcatc tgaggtcagg
12961 agttcgagat cagcttggcc aacatggcaa aaccctgtct ctactaaaaa aaatacaaaa
13021 attagccaga tgtggtggca ggcacctata aacccagcta cttgggaggc tgaggcagga
13081 gaattgcttg aacccaggag gcggaggttg cagtgagcta agatcacgcc actgcactcc
13141 agcctaggcg acaagagaga aactctgttt caaaaaaaat tagctgggcg tagtggtggc
13201 acacacctgt agtcccagct actcgggaag ctgaggcaga agaatcccct gaatctggga
13261 ggcagaagtt gcagtgagtg gagatcgcac cactgcactc cagcctgggc aacacaccaa
13321 gactccgtct caaaaaaaaa aaaaacaaaa aacaaaaaac aaaaaaccaa aaaaacaaaa
13381 aacagaaggt agagtaatgg ttaccaaggg ctaggggcag aggaaaaggg tgagtctgtt
13441 gtttaatggg cattgtttca gttctgcaag atgaaaaagt tctagagatc tgtttcacat
13501 caatgcttga aactactgaa ctattaattt agaaatggtt aaggtggtaa attatatgcg
13561 tttttaccac aataaaacaa ttcattttaa aaacaggccc caatcggcat ggtggctcac
13621 gtctataatc ccagcactct gggaggccaa ggtgggagaa tcacttacag tcaggagttt
13681 gagaccagcc tggccaacat agcaaaaccc tgtctctact aaaaatacaa aaattagccg
13741 gcatggtggc acgcgcctgt aatcgcagct actcgagagg ctgaggcagg aagaatcact
13801 tgaatccagg aggcagaggt tacagtgagc cgagatcatg ccaccacact ccagcctggg
13861 cgacagagtg agactctgtt aaaaaaataa aataaaataa aaaataaaaa ataagctaga
13921 cacagtggct cacgcctgta atcccaacac tttggaaggc tgagatggga ggatcgcttg
13981 agcccaagag ttgaagatca gtccaagcaa tgtagtgaga ccccttctct acaaaaaatt
14041 taaaaacaca ggcacagtgg ctcatacctg taatcccagt attttgggag gctgaggcag
14101 gaggacttag tcagctcagg aattccagac cagcttgtac aacatagtga gaccctacct
14161 ctacaaaaaa ttaaaaaatt aaccgggcgt ggtagagtgt gcccgtgatc acatcactgc
14221 actacaacct tggtgccaga gcaagactgt ctcaagaaaa aaaaaaaatt taaatttagc
14281 tgggcatcat ggcacacgcc tgtagtccca gctactcagg ggtggaatga ggcagggtgg
14341 cagctgagat gggaggatca ctggagcctg ggaggtcgat gttgcagtgg gccataattg
14401 tgtcactgta ctgcagcctg ggcaacagag cgagaccctg tctcaaaaac aaacaataaa
14461 aaatgggcga aggatttgaa cacttctcca aagatacaca aatggccaag cagcacatga
14521 aatgctgctc aatatagtca ttaaggaaat gcaaatcaaa atcacaatga gctattactt
14581 cacatccact aggttagtta taagcaaaaa caatctcaag cgttgcagag gatgtgcagc
14641 tatctgaacc ctcaaatact gctggtgata atataaactt gtactttgga aaagtttggc
14701 aattgctcaa aatttaaata tggcgttacc atatgaccca acaatatcac tcctaggtat
14761 atacccaaga gaattgaaaa tataacacat atataagcta gctggaatat agccataatg
14821 ctgctacaaa gtgcaaacaa ccgaatgtaa attaattgat aaatgaataa acgaactgtg
14881 gtatatccgt acaatgaaat actattcagc cataaaaaaa ctaagtacaa acaagcatac
14941 aatacggtgt accttgaaaa tacactaagt gaataacacc agacacaaaa ggccgcatat
15001 tttattattt tacttataca aaatgtccag aataaggcaa attcatacag acagaaataa
15061 agtagcagct gccatgggct cgatgaagaa gaatgaaagg aatatagcag gtttgaaaaa
15121 tggcccccaa aatatcaggt cctaatccct taagcctgta aatgatacct tatttggaaa
15181 aaggatcttt gcagatggga aaattatcct ggactataca ggtgggccct aaatgccacc
15241 acaaatgtcc ttgtaagaga agcagaggga gatttcacac atactctagg aaaaggtgat
15301 gtgaagacag aagcaaagat tagagtgaca tggtcacaag ccaaagaacg tcacatccac
15361 aaactagaag atgcaaagaa tggattctcc gctagagttt cccaggggag gacagccctg
15421 ccaacatctt gatttaggtc ttgtgaaact tattttagac ttctgacctc ccgaactaag
15481 ggagaataaa tttttgttgt tttatgccac caagtttgtg ataatttgtt atagcagcta
15541 tggaaaagta atatagggag tgactgctaa tggatacaag gtttctttag agttgatgaa
15601 atgtcttgga attagatatt gctgatggtt gtacaatttt gtaagtatat aatactaaaa
15661 accattcaat ttcacaccgc aaagggtgaa ttttatggca tatgaactat atttcaaaga
15721 aaaacaaatg cctagtaaca ggctggtata acccSccatc tcccaacaac tgctgctaaa
15781 ttttcaaagc accattaagc agactcaatt cgtagcaagg ataatggaac aatgcaagct
15841 catctagcga acaaaaagaa ttaagctagt atcaggtggt caatttacaa actgtagtgg
15901 gagtcataca ttcccaagga tggaacagtt atatctcctt gcagagtgaa ctcttctccg
15961 ataccaagga cttggaagac caaataaatt atacgtagga ttaccaaata tgctggtatg
16021 accaagacag ttatagttta aacttctatc tctgtggaat tatggatagt gccactcttc 16081 aatctttaat aagtccttca gtttagatga taaattacat agtcaaccta gttataagga 16141 atctagaggt tgtgaaaagc tgtgagagca tattctatgc agaccacata ggtcctatca 16201 tgactcccta tgtaacttgg gtctctgcga ctgggaagct cccagggaaa aaaccccagt 16261 taagacttct gcagagcatt cacattttgt ttcaaactta tacaacacta atgctaaagc 16321 tcaactctca gaattgtatt attcagtatt atccaaataa ctgattaatt tacacactga 16381 agggaaagaa caaggaatgg acctatacac acttgtagtt tacaagtata agaagctaac 16441 aaagaataag attaaaataa cttgtgccag gccgggcatg gtggctcacg cctgtaatcc 16501. cagcactttg ggaggctgag gcaggtggct cacgaggcca ggagtttcag accagcctga 16561 acaacatggt gaaaccccat ctctactaaa aatacaaaaa cttagctgag cgtggtggca 16621 cacgcctgta atcccagcta ctcaggaggc aggagaattg cttgaacccg ggaggcagag 16681 gttgcagtga gctgagattg tgccactgca ctccggcctg gacaacacag caagactccg 16741 tctgaaaaaa aaaaaaaaaa aaaaacaaac aacttgtgcc cacattaact aggtgttaaa 16801 agccaaaagg tgaaagtcac tattctcttt tcacatatca aattgaccct aaggtaatat 16861 tgctgcatta gaactgagct ggtgggaggg atgttaagaa attagggaga aattgttaat 16921 ctacagaaag accatcatat aaggttctgt gtgtagccaa acatacacat ttgatgttct 16981 gatgtttgct attttagtgg aactatagct ataaaatgta ttcaatcagt agaattaaaa 17041 catatcccag tccacaaaaa actaagagag aaattataca ggaatgatca agaatctgca 17101 cagttctcat aatctcatta ctagagagaa aaggagaaag ggaagcatca aggtagtatt 17161 tgagacgaat ttaagaagac cactcattca ataaaagttt ggtgggccaa caaggtgcca 17221 tgtattggtc tggtgatata gcagtgatcc aaagtctttc acttcaggga gctgacattc 17281 cagaaggagt agtcagaaaa taattttttt aacaaaagta acatggttat ataaacacta 17341 gggagaaaaa taaagtagga aatggaggca ggttgatact cacataacat gaatatggct 17401 gtctggggaa gaatactcca acaaagggaa cagcaaacac aaagcctctg agactggagc 17461 acttaacaca tgcaaggact gatcaatagg ctaacacagg tggaccaatg aaaagagtga 17521 caagttagaa atgagataaa aaatgagctg tttgtggcaa aggaaagcca agatcatgta 17581 ggccattcta aattcttcag ctttggccag gtgtggtggc tcacatctgt aatcccaaca 17641 ctttgggagg ccgaggcggg tggatcacct gaggtcagaa gttcgagacc ggcctggcca 17701 acatggtgaa accccatctc tactaaaaat acaaaacgta gccaagtgtg ctggcatgtg 17761 cctgtagtcc cagctactcg ggaggctgag gcaggagaat tgcttgaacc cagaagacag 17821 aggctgcggt gtgctgagat catgccactg cactccagcc tgggtggcag agcaagactc 17881 catctcaaaa aaataaataa aaataaaaat aaaaataaaa taataataat acaaaaatta 17941 gctgggtgtg gtggcgggca cctgtaatcc cagctacttg ggaggctgag gaggagaatc 18001 acttgaaccc aggaagcaga ggttgcagtg agccgagact gcgccattgc actccagcct 18061 gggtgacaga gcgaaactcc atctcaaaaa ataagtacat aaatagacaa aaaataaaaa 18121 caaattcttc agcttttacc ctgagtgtga tgagagatta ctggaaggct ctgagtgaga 18181 agtaatatga tctcactcat ttcattagga ttgctgtggt tactgtgtgg agaatagact 18241 ttagagagta atagtggaag cagaagacaa gaagctactg aaatattata aacaaaagat 18301 gatggtagta tcagacagga tggagaaata gagatactaa gaagtcggac tctagataaa 18361 aatctagaag gcccaattca ttgatggatt gggtttggga tgtgaaggag aggaggagga 18421 cacacaagag gtaatgctga gcaggtaaat ctatgagatc agagcccaag gaaagttccg 18481 aggtaggtac ttaaattttg aagtcttcag tatgtagcat tatttaaaac acaagactgg 18541 atgagatcac tcagggaaaa gaggtctgag atctaagctc tggggcattc cattgattag 18601 aggtcatgga gatgagaagg agctggtaaa ggaaattaaa aaaaaaacaa ttattcacag 18661 ccattcaggt gggaggagaa ttgagaaaga gaagcatccc agaatccaat ggggaggaga 18721 aaaggaactg taatatatgc tgccaataga tcaaataaaa tcacgaaaat caactaaaac 18781 tgaccaatgg attaagcaaa gtggaattca ttggtgacct tgacagcagt gggtttaagg 18841 aagaacgaga acaggagata gcaaatacgg acaactctgg agaaattttc ctgtaaggac 18901 aagcaaatac aaattaaaac aagcagatgg gagatgtggc atcaaaaacg agatctttaa 18961 ggtgaggtga tggtacaatc agtatgtttg ctatattaat gatctggaga gatagaaaac 19021 ttaaccctag acaaagagaa gtcttgctag aataatatcc ttaaataaac aagtgcacat 19081 gtagaggttc tggccttagc aagaagtcaa attattcacc cagagtaaca ggacaatgct 19141 ttgagatcct atggaaaagg gttaaatggt ggataaatgg ttgcggtggc catcctccaa 19201 gatggcccta aatgaatccc acttcctgga attcacatag ttgtagccct ctcccactaa 19261 cagggttggc ctgtgggatc aagcacatac agcaggaagt gatggtatgc cacttcagaa 19321 attaggtcat aaagactctg cccttcatct tgggtacaag gtctcttgaa tcatttgctc 19381 tgaagtaagc cagctgctgt gacatgaaca gccccatgga gaggcccaca gagcaaggaa 19441 ctgaggcctc ttgccaacag cccagtgagt gagcttagag gcagatgatc caaaccaagt 19501 caattccgca gagactgaca atccagccaa Rggtttcact gaaacctcat gagtgacctt 19561 gagccagaaa cagccagcta agccactctt ggattccaga ccttcaaaaa ctatgaggta 19621 ataaattttt ttttaaacta ctaagttttg agacaatttg ttacacggca atagataact 19681 aatacaatgg gtctcactga gctgtaatca caaattgctg ggcacacaat tcactataat 19741 ctcacctact ggcatgcaat gacttcagat gacctcccct gtcctgctgc atgcagttac 19801 ctcatgaaat acagtcgctg agaaagaggg taggataaaa ggagtcagct ttatataact 19861 taacatccct gatgctcttt tttttttttN aagagatgtg gtaaaagaat gccactacca 19921 ccctctcctt tctcaacaaa gcaatttaga gggttcccga atgacctaca tcaacaattg 19981 gcctgaacta atcaaacRca tcattcagat tccctcagta aaatattgaa taccaaacag 20041 ctatgYttca gcaaaggaaa gggcaRggga gcgaaactga aggacgaaaa tacaactttg 20101 gtcctcatca gggagacttc tcttttctta cctcaggttt attacaacgc aacttttaag 20161 aacttgagct attccaggca gaaggtcaca tccttgaggt aaccagagag aagcattagt 20221 tacttacatg tcacaagaat attttcgatc ccactgcctg acattgccat ttaggtgttt 20281 atcaatcatc tcacacttaa ccagataagt gaactgaact cctttccccc agatctgttc 20341 ctccaatcta ccctacctta gcaaaggaaa acgtcccacc cgccaactgc ccaaatcaaa 20401 agcctgcaaa tcacccgagg tcctggctct cacgggaggg actccattag ggtcccaggg 20461 gctgcattaa gagtcccgac agcgacgcag taaatgagac aagacgctgc ggtaggggag 20521 agaNNcccat acattcaaat gccttaaggg gtctgggcct acaggtctcc agaactgggg 20581 agggcctggg gttacaaagc agttttcaga tttttaaata cattgagcgc caaaagagac 20641 cccgcggggg cgatggggga ggggagcgca ctgggagcca ggaaaggccc cttctccagg 20701 aggcccgggc ccagcccggg ggtgggggcg tctgcgtgag ggagtggagg ctgatgtaaa 20761 agtggcgccc gctgagatta gggaaggggg tgtgcgtgag gggatcgtgg gacctgSttc 20821 tgccctgcac tcccccacag aaagcggctg tattgcaaag gccgctccgg cagcgcacgg 20881 ccaagcgcgg tgtcgcgacc cgcagggctg cggctcgcca tgaaatccct caagtctccc 20941 ctcagggaac gctgaagccg cgccacgccc ccgtccttac cagtccggat cagctgctgt 21001 tcgcgagctg ccggccacgc accagccccg gaggcgctcc cggggcacag ccggcggcga 21061 ctacgcctcc tcaggccccc ggcgccgccg acgcgcacgc ctccacacgc gcgcgtccag 21121 tggagacctg cgattggctg ccaggtgccg gcgcgagatc ggcgcggctc cgagctagga 21181 gcatgcgcgc gctctgacgc ccctggtggc gacggotgga cgcggggtta aattgagaag 21241 gaggagggca gcagcaatac ccctgaggcc ttgaaaggat cttgggttcc ggatcacggt 21301 tccaggcagt cagggttcca gagctgaggg ggtatccaag cagtcagggt tccagagctg 21361 aggagcatcc aggcagtcag ggttccagag ctgaggggca cccaggcagt cagggttcca 21421 gagctgtcgc agccagggct tggggtgggg ggtaggcttc cagtccatca ggggatggtt 21481 tgcccaccag aaggccagct gttataattc tcaggtgtgg tccctttgtt gactatccat 21541 gcttaaggga ttgtgaggaa acaactgaac ctaacatctg tcctcttcta gttacatctg 21601 gggccagttg aatccagcct gccacctgtt tttgtacagc tggcagcaag ctaagaaccg 21661 atttcacgtt tttttaatgg ctaaaaagag aatattttgt ggcatgtgaa aattatatga 21721 aattcaaaat tcagtgtcca taaataaagt tttattggaa caacaaacat gcccattcct 21781 ttcggtatta tttatgcctg ttttcctgct tcaacagcaa agttgattaa ttgccagaga 21841 ccatgtatga aaagcttaaa atatttactg tctggccctt tacagaaagt ttgcccactc 21901 agtttacttg cagctttgat tttttttttg aattgaccaa ttctgccaaa agtctatttt 21961 agcaatcttt ttggggggcg ggtaaatcgc ttttggttta attaatctat aatgttattt 22021 tcttatctat tttatctatt tcttccattc tgttattttc ttccttcgtg acttcatagg 22081 cttgttctgt tacttctcat tacaactttt ggagaaaaat gcctacctcg tttgcttgta 22141 aacttttttg tttctcttgt atttaatgct ataaatttac ctttaagtac cagtttaact 22201 gtgtctcacc agtccgcttg gtaatatgtt cattatcctt caattagata tgtcctaatt 22261 ttgctagttg ttagaaatgc ttgttccctg gtgccgcaaa gaaatagcac ttgaacataa 22321 atttaatttt ctcagcaagg ccattttcac tttctgcaga aagggtacac tcgccagcag 22381 ttttgccacg agagtacccc aaacaaagga tacagggtta tttataacct gacgcatcca 22441 ccctactgct gtgtccggtt tccattggct ggaacaggac ctcacattct gtgtttgtcc 22501 cgattggcta acaacttaga actttctaaa agaggcaaag gcagaggaga acaaaggaag 22561 gaggatgtaa cttgtagaat gctgagaaag gtaaaaacac ctccaaatag gaagaggaac 22621 aggctatgac ctaatgcttg cttggaccag tataagcatg caagggcaaa tatttaggct 22681 aaattgtggg agctaagaac acaaagtata ttaatttctt tattacggct agcagatatc 22741 taagaatgtt agcacaggtc tttgaataaa ttttacttct aagagaagtt actatttatt 22801 cgtaattaga cagggaggaa agtctttgaa gaggaacctc tactttattt tttacatagt 22861 gattctttct aacacaaggg ttatatagta tatgttattt atttttgaaa tctatgagat 22921 ttttaaaaga ttttttaatt gtattataat caaatcacat ggcctaaata ttactcaccc 22981 ttgcatttga agatttctgt tcagtgaagt gtaccatagt caaaatgaag agatcataga 23041 tgtaaagaag atatttgcag tatctaaaat gtatatatga ggctgatatg taaagatatt 23101 tgcattatct aaaatgtata tatgaggctg atatgtaaag aagatatttg caatatctaa 23161 aatgtacgta agaggcctgg cacggtggct cacacctgta atcccagcat ttttggagac 23221 caaggcaggc agatcacttg aggtcaggag ttcaagacca gcctggccaa catggtgaaa 23281 ccccattttt gtacaaaaaa tacaaaaatt agctgggtgt ggtggcacgc gcctgtagtc 23341 ctagctactc gggaggctga ggcatgagaa ctgtttgaac ctgggaggca gaggttgcag 23401 tgagctgaga ttgcaccact gtactccagc cttgggcaac agagcgagac tccgtctcaa 23461 aaaataataa catgaaaaat aaatctatga gaaatgatct tcttttggct tcataataat 23521 tccaatgtcc atcagtaaat tagtatcaac taagctccag gctttattca aatgtcacta 23581 gtttttccca tcatcctttt tgttccagat tcccacccag gatactgaat tgtacttggt 23641 cattttgtgt tcttagcctt ctgtggtctg tgacagtttc ttaaactttt cttgcttttc 23701 atgaccttga caattttaag aagtaaaggt caggtatttt tcagagtgtc tctcaattta 23761 ggtttgatgt ttttctcatg gttacactgg ggttatggtt tttagaagac tatcacagac 23821 atgatgtgtc tttctcataa tttctcatca gcgatccatg ctatcaataa gacttcctac 23881 tggcttctcc gctgtaaatt acttcccccc cctttcatac cttattctaa atcttgccta 23941 catgcaggaa ggccgtgagg tctcattgaa ggaaaattat ctacacaagt tactttgcaa 24001 ttcttcttta aggaagattt gtctcttctt cctttagttt taaattaatc aaaaatagct 24061 ttttagtttt aaattaatca aaaataaaat aaaaaattta gtacctcagt catacttgcc 24121 acatttcaac agatcaatag tcacctgtag catattggac agagcagaag tagagcattt 24181 tcatcattgc agaaagatct acggacagca ctgacttaga tcattagtca acctttcttc 24241 ttcccaaata tatgcattta gaaatataaa ttcccatgaa atgctttagt tccatcccat 24301 aaattttgtg gctgttttca atatctttca gttcaacatt ttcctttttt cactgcaatt 24361 tcttagagga gctatgggtt attcagaatg aggaccagga ctggggtgag ataagcaagg 24421 aggctgaggc acattaagga gagacccacg cccagcttca ggtatggcat attgtcacct 24481 taggttccct gcttgcctca ccctagtccc agccctgttt agaaatgtat tgcctaattt 24541 aggaactttg ggtttttttt cagtgatctt tctgttactg atttgtaagt tgattcccgt 24601 gtactcagtg ggtaactaca aagagcccca cagctaacat cacaattaaa gtaaactatt 24661 ctacctgagt ttgagaccaa gaaaagcaca cccatgaaag ccacttcaat tcgcaaagca 24721 tttggaatag ccaaggcaat tttgaacacc aaagttggag aaatgaaact accacaYgga 24781 ggattatgca aagctacaat aattaagata ctgcagcatt agcacaagga tagagaacag 24841 gacaaatgaa atgaaataga ttccagaaac acatatatac aatcatacct gattcacaNN 24901 gtttccacgg caatacagta gggaaggagt ggtcttttta gtaagtggtg ctgattgatt 24961 ttgggtatgt atatgaaaaa attagtcttc atgccttatc acaccattaa caaaaaatca 25021 gtttgagatg tctcatcaaa acagtaaagc ttctagaaca tagaaggaat atctttatgt 25081 cgtttgtaga caatttctta aataagactc atgaaagata aaccataaaa ccacgattta 25141 gatccaaata tgggacattt ccagtgcccc agcacccttc ccagtcgaca gcccagtttt 25201 taaaaaggta ggagggggca aaacttgaat agacatttaa taaaaagaaa tactcacgtg 25261 gccgataact atataatctc aaggtccatt aatcctcagg gaaatgcaaa ttaaagccac 25321 aataaggtac cactacaatt tcaagagaag ggcacttact aaaaggactg acagtaccaa 25381 gtgctggatc agtagcaRtg gaattcgcag tatcggtggt gggaatgtaa atcgtttcaa 25441 tcactctgga aatgtatttg gcagtatcta cactagacag ctaaatatat gcagttccag 25501 cacttctcat agctatatac acccacgaga aatgattaca tgcttaccaa aagacacgca 25561 caagctcata gcagctttat tcaaaattgc caaagaatca gcctcaaagt ccatcaacag 25621 tgaaataaat ttatacaaaa ctgccacagt cactcaacaa aattctaccc agcaatgaaa 25681 aagaatgtgc tactgctaca cacagcgaaa atgcatctca cttattttat ttcattttac 25741 tttattttga gacagtcatg ctctgtcacc caggctggag ggcagtggcg cgatctcagc 25801 tcagtgcaaa ctccccctcc caggttcaag cgattctcct gcctcagcct cccgagtagc 25861 tgggactaca ggcgcccacc accacgcttg gctaattttt ctattttatg cagagaaggg 25321 gtttcactat gttggccagc ctggtctcaa acttctgacg ccaagtgatc cacccacctc 25981 agtctcccaa agtgctggga ttacaggcat gagccatcac Rcccagccat ctcacttatt 26041 ttaaaaagcc aaagacaaga atctttgtgt gattccattt atttaaagtt catRaacaag 26101 aaaaacctct gaggtcagaa taagggatgc cYtgttggga gcatttgact gggaaagggc 26161 tcaaagactt tggagctact tcttgacaat agggagctga tgagatgtca ctgtRaaaaa 26221 caacctgaca gttgatgaca ttgcttgcta gcatctacag ccacaatgca catatggcct 26281 agcctcactt ctgcctttcg gaactcctgg aagtcagtcg aacatatttt cagctagaat 26341 ccttgctgca agggagtctg gaatctctag tgttcatagc cgtcttcttt ttagttttta 26401 caggagaaga ctgagggtca gtgttgctaa caataacgga agccatacca cttgatcctg 26461 gctgaactga agtttgaata ataacttttg tttcacgagg Saacccttgc agttgctccg 26521 gctttttaaa tgtttgatgg tccttggttt tgtgctctat ttcctccttc aacgtcaaaa 26581 actgtagccg gcacttggaa cactgaagga ccctccttct gctgtgcctc caacaatgat 26641 tcatgtatgg tattgcagtt ttgaaaagtt tgagacaaaa cagacaaagc aaattctttg 26701 tgttttcatg gcacgttcta aaatgtgttt ccacatcagc aaagaccgac gatctgtaat 26761 ggcaaacctg gcacacRtag ggcatttcgc caggcttatg atggtccttc atgtgttgta 26821 agaggacctg atctgtttca aatgacaatt cacagatttt acagacagca gagggcccca 26881 tggcgatgtg tacactatca atgtgacact gtagctggaa gggagtggga aactgccggt 26941 ggcagtgctg gcaggtggtg tggtcttccc agctgtcgtt cctctgcttc tcaaattcca 27001 aatgatgctt catgtgattc ataaacttaa tattttttag aactttcacg cagctgaggc 27061 atttaaaggt ggtgtgagtc ttctgttccg gctgcccatc tcctttatgc tgtccatagt 27121 aaaagtYgct aagtaacacg atgggatttt ctttcttggg atcaaaggtc ttgttttgac 27181 ttgctagact tgaaatgtct gtcattgcca ggccagactc atttgctctc tctggatctg 27241 taagattgaa atgtgccttt ccattagcat caggccaagg aaatgttact ccattctgaa 27301 catggtttga tgagttgcag atcccctgtg agggtgtatt tgtgcttatt gtagaagaca 27361 tatctgaagg gaccacagct aaagaaggta cccctgggat tccatccctg agtttaaccc 27421 ttttggaatc tctgctgttt atatctgaag tggaaagtcg ctttgaatca ggagaactct 27481 catttctgcc tcctccagag accatagctc caactagaca ctgagtccct ggggggagcg 27541 aatctgagta aYtgggagaa acaacttgtg gtgagctcat tttataacca ggttcagatg 27601 aagacttcat agtgacagga ctatctgtcg atcgcccctc agacagagaa acSggcatga 27661 tggcttttgc catagaggtc acatgatttg caggctgcga cacgtgagca ggatattgac 27721 ggaagtggcc tttctttctt cttgaMtttg agcctggggt gactctgttc aaaatgtttg 27781 aaacgactgg ttttgaattt gaaatcatcc cgacaaagag aacttcagca tctctatgta 27841 catgctccac cccaacatag atcagatctg gatcttcatc atcctcctcc ctttgtttgg 27901 attctctcaa attcttcttt ggtgattcca ctttcttaca caaaaagata tctcccattt 27961 tcaatttact ttttgcttga agccactggt ctcttcaact tccagagctg tctttttaca 28021 aattgccacc taagtgcaac catgtgacaa tagtcaatat ttatttcgaa agacaaaatc 28081 attactttat tgtatcctcc cttaaaacac tgagataacc atcaacatct ctattttaca 28141 cgtcagaaat Raggcttgaa acattttaaa tggcataata atttcaaatt tacctgtctc 28201 tcctcccctg gtcttctgca gttgtgaggc aaaggaagca agatttcagg aggtgcagat 28261 ggaacatctc agagtgcttg cctctaaatt ttgcactcta gatgcctcac tcacctcctc 28321 ctcatcccac aggtactctc tcagatgtct aaggtttcta accaacttga acaaaacttc 28381 tctcaaaaac attctgctcc tagcctgggc aacatagcaa gaccccatct ctacaaaaaa 28441 atttaaaaat taaaaaaaat tagcttggca tggtggtaca cacctgtggt cccagctact 28501 tgggaggctg agccaagagg aacacttgag cccaggactt caaagttatg ttcatgccac 28561 tgcactccag cctgggcaac caaatgagac cccatcacac aaacacacac acacacaaat 28621 cacaacacac acacacacac acacacacac acacaccctt tgctgttcct gtaatgttcc 28681 ttttctcagt aaactcagtt gctcagtcta caaacttcag agtcatcctt tgccagactt 28741 tctctcatac actacagcca atcaattagc aaagctagtc tgctctatct tttaaagata 28801 tccagaatct gactactctc ttccacatct actgccacct ccctgatgga aggtaccatg 28861 ttctctccct ggatcactgc agaagttgcc agtcttaaaa gttttgtcag atctccccat 28921 ggtggcccca atgcccctgt taaaatatac aggggagcaa gtatatttYg ctcttaagtc 28981 ttcaatgact ttccatttca ttcataggaa ataccagcaa actttaaatt tctccatgga 29041 accttaggtc atcatcgccc ttgctaactc ctctgttgct ctgatgcagc catcctgagc 29101 tccgtgaatc tggaacacac caggcctttt tctttttcag aacttttgtt gttcccggac 29161 Rttgcgtgcc tctttcccca gattaacccc atctgggctc ctggtctgcc tcagtcccat 29221 ggttcctctt cagcaaggct tacctgacca caatatcaaa aatcacaacc tccttaactc 29281 atatgttcaa accctcaatg ttatttttca acttagcatt tatcactgcc taccacacaa 29341 tatgtaaaat atatcttgca agcccaggag tttgagatta cagtgagctg tatcatgtca 29401 ctgcatccag cctgggtgac agaggaaagg aagactctgt gtcttaaaac aacaacaaaa 29461 aatgggacag gtgcagtggc tcgtacctgt aatcccaaca ctttgtaagg ccaaggcggR 29521 aggattgctt gaggtcagga gctcgagacc agcctgggca acatagcaag acccatctct 29581 acaaaaaatt taaaaatata acttgtttct tttctgtgtt tacccactaa actgtatgcc 29641 acaYgagggc agggagttgt gtttcattta ctcctcagct tatagagcaa tgtctgtcat 29701 actgtgcatg ctaaatcttt gttaaataaa tagtagctgt tattaatatg atcatttagg 29761 tgtaccagag gctctaatcc attattccag attctctaag catttaatga aatccaaatc 29821 caaatgattt aatactgttg tacaagggag aaggtaaacc tgtgtgtatg tgtgtgtaaa 29881 ttgaaacaaa agttttgtca aatactctaa taccttcacc ttaccRcaag ggataggctc 29941 tgataccttc tatgccatat tttattgatt ctaaggtata ggcttagatt tccattttaa 30001 catctctaaa tcagattgca tctcatcata gcatctcatc aatataaaat acatttcaca 30061 atttgttttt gcagtatata tttttatttc atagtggaat caaaaatgac atatctcaga 30121 tccttttgtg aggataagcg tgatgcttgg gttttcactc atgtgagatg tgcttccctc 30181 aaaccttgtt atatcagcat gtttaacatc tgatgtaaaa aaaaaaaaaa aaaaaggaga 30241 gtaatgtatc ttttataatt gatggcatct tttttttttt ttttttgaga tggagtctcg 30301 ctctgtcgcc caggctggag tgcagtggcg cgatctcggc tcactgcagg ctccgccccg 30361 cggggttcac gccattctcc tgcctcagtc tcccgcgtag ctgggactac aggcgcccgc 30421 cacctcgccc ggctaatttt ttgtattttt agtagagacg gggtttcacc gtgttagcca 30481 ggatggtctc gatctcctga cctcgtgatc cgcccgcctc ggcctcccaa agtgctggga 30541 ttacaggcat gagccactgt gcccggccaa ttgatggcat cttatagtca gtgaaaaatc 30601 ctatttcttt ttgtaagctg acagcMcact aaacttgttt tgtagttctc taatggatat 30661 caSctacatt ttggcaaaca atggtttaag acagaaattg gaaaattggc ccttcccctg 30721 tttttgtaca gtcaggaagc caagaatgtt tcttacattt ttaaggtatt gttttcttac 30781 ttttttttta ttaagatttt tttaaacttg tacattttta Ratttggggg aataaacaYg 30841 caaaaggaag cggggcgcgg tggctcacgc ctgtaatccc agcacttttg ggaggccgag 30901 gtgggcggat cacttgagct caggagttcg agaccagcct ggccaacatg gtgaaacccc 30961 ttctctacta aaaatacaaa aattagccag gcatggtagc agatgcctgt agtcccagct 31021 actagggagg ctgaggcagg agaatctctt gaacctggga ggtggaggtt gcagtgagcg 31081 aagatcgcac cactgtgctc cagcctgggt gacagagtga gactgtctca aaaaaaaaaa 31141 aaaaaagaaa aaaggaatat tttgtgatgt gaaaattata taacattcaa aatgtcagtg 31201 ttcactataa agtgttgtgg aacacagcca taatcatttt tttagtgctg ttgtccatgg 31261 ctgctttcac acttctatga cacagttgag tacagtagta cccccttatc tttggggcat 31321 atgttccaag acccccgaag tggatgcttg aaactgcaga tggaaccaaa ttcaatatat 31381 actatatttt ttcatatatg tacataccta tgatcaagct taatttataa ataggcacag 31441 taagaggtta agcataacaa aataattata acaatatact gtaataaaag atatgtgaga 31501 tagtgcgtgg tggctcatgc ctgtaacctc agcactttgg gaggctgagg tgggtggatc 31561 acctgaggtc aggagtttga gaccagcctg gccaacatgg tgaaacccat ctctactaaa 31621 aatacaaaaa ttatctgggc aKagtggcga gcacctgtaa tctcagccac ttgggaggct 31681 gaggcaggag aatcacttga acccgggagg cgtgggttgc agtgagccga gatcgcgccg 31741 tggcactcca gcctgggcgg ccagagtgaa actccgtttc aaaaaaataa attaataaat 31801 taaattaaag gtatgtgaat gcaatctctc gctgacaaaa tatctttatg tactgtagat 31861 cttggcaacc ttagcatacg attttgtttt ctttccttat tatgttaaaa actttcccat 31921 tttcacttaa aggaagcaag cactttacag cttctttttg gcatatttca atcgccagca
31981 t'tgctaccct tgtactttgg ggccattatt aagtaccata agggttaatt gaacacaagc
32041 actgagatac caccaggaca gtcactctga taactgcgac cgctcctaag tgactaaggt
32101 acagagagcg cagccagcca acagcatggg gactcaggac aaagggagga ttaccatccc
32161 agggcagggc tgactcaaga gcttatcaca ctactcagaa gggctggcga cttaaaattt
32221 atgtattgcc tatttctgga attttccaat taatattttg gaacctcagg taactgaaag
32281 cacagaaagg aaaaccctgg ataagggaag actactgtag ttgRagcaga gaattgcaat
32341 Rcctaaaata tttgccatct agtctttcac agaacaagtt tgccagctct agttttagag
32401 gagcaaaatg aaagatagca aatttctcag ctcaccagtt cctaaagttg cgagtgcatc
32461 cagatgattc agtgcagctc agtatgttcc actctgggcc acaagggaaa gcgaagctgg
32521 agcttccttg tctccacctc cSggagaaat ttgtccagag accggcagca ctgcttccgc
32581 aggcgaacaa cgccgcttgc tcttcaaagt cctttctgag gccagatcag aaggtcccac
32641 tccttttaag ccccttattg cccaatcatt tttccagtca cgtgtctgaa gcagtttatc
32701 ctgattggat tttcagttgt tgtccagctt gattgccaaa agttttcctc caattgctac
32761 tcaaaggaaa gaatgagggt ggggcttcag ggctgcYttc caggttgatt gagctgStga
32821 tgggcatcct ccctcctctc tctcttgttc agagtctacc actttctctg cagtgtgcct
32881 gggccctcag ctattgggga tgagaaggga tgcggagtgg gaggcagtcc acatgtctgt
32941 tccaacaaag ctcagaggtg agcgagttRa aaagcagatg tctttccagt aggagcccca
33001 ggggacaggg tgggtctcac agcccttgaa gaccgagctc ccgtaggagt ttccaSgtgt
33061 ccaaacaggg tcctcactgt gagtcttcaa cagctttctc caagcagcag cccactccct
33121 tcttaatcca tttccagggg tcaaaggtct atcctggttc cctcccatgc cctctcactt
33181 ccttccctct gtctctccct cctccctcct ccttcactac cccctccctg gaaccttctc
33241 agctgaattt caggcccacc ctttcactct ctttacaggc tttgcaacac agattagaaa
33301 gcgggggagg gggagcctca agagtccctt gacaggtaag aaatatttat atttccaccc
33361 ccattccaac gcaatgcaat caagtctcaa' aattaaaacg aaaatcattt gcaaattaaa
33421 atgttctaaa gtaccatctc ctgataaaac tttaaaaata cctttctggg ccggatgtgg
33481 tgactcacat ctgtaatccc agcacttttg aaggccgagg cagcagatca catgagccca
33541 ttagtttgag accagcctgg ccaacatgat gaaaacctgt ctcttattct ttataaagta
33601 aaatacaaaa atttcctggg cgtggccgtg cgtgcctgta atcccaggta ctcgggaggc
33661 tgaggcagaa taatcacttg aacccagtag gcagagccca aggtggtata aaagtgtgtg
33721 acccatggtc tttattcttg tctagggact ggggaaaggc tgcttgaggg cattgcaagt
33781 agccaagagc atgMccctgg aaaggaaggt gaaatgaggc ccgctactga ggtacacctg
33841 tagtcccagc tatcaaaggc tgaggtgaaa gatctttgag cccaggaaat ccagacaaac
33901 ctcagcaaaa tatcaagacc tctgtttcta caaaagaaaa aaaaaWtttt tttaattagc
33961 caggcattgt agcacgtgcc tgtagtccca gctacccggg aggctgaggt gggaggattg
34021 cttgaaccag ggagatcgag gttgcagtga actatgattg catcacatac tttagcctgg
34081 atggaagaga gagaccttgt ctcattcatt cattcataaa taaataagca agcaaagtga
34141 aatgaagtgc aagacttaga cagtaaagtt ctaacagggg ccgggcgtgg tggctcacgc
34201 ctgtaatccc agcgcttagg cagaggcagg caggtggctY gagcccagga gtttgagaYt
34261 agcctgggca acctggcaaa accgtgtctc cacaagaaaa tacaaaaaat tagcctgggt
34321 gacagagtga aaccctgtct caaaaaaata aaaaaagaaa aaagaaagga agacagagaa
34381 agaaagaagK aaagaaagag aaaaaaagga aggaaagaaa ggaaaggaaa tgtgtattct
34441 gcagaactga tattcactcc ttcctctttt ccagaagcag aatcctgcta ggaagattcc
34501 aggaagccta ggcagtggtc aggggctggc tcaggtataa agcaggccct tcccctgaga
34561 cagaaggttg tggcaacaat gcttgtggga ggtgagaaaa ttgagaggag tgtctagaaa
34621 tagctcggag ttgcatctta ttttcttttg ctcagtttta aaagtcccaa ataagaccac
34681 atctagctgt tctaaccaag ctattctaac ccactaatct agtaaaaata aaatcacatg
34741 gtctgaatat aaatttgcat caggatacat gcctttccta tgaaacattt atgtttcctt
34801 ataaaatgta tcctcctttt ctgcagaaaa ctaactgtaa tgcatccaaa ggtcacagaa
34861 tgtggtgaaa catcctcatc attgattttt Waaaaaattt ttatttattt atttatttgt
34921 tttttgagac atggtctcac aYtctgttgc ccaggctgga gtgcagtgga acgatcatgc
34981 agtcttgatg tcctggaccc gagcgatcct cccacctcag Yctcccaagt agccaggaca
35041 ataggcagat gccaccatgt ctaactgatt tatttttatt tttgagtaga gatgaggtct
35101 cactatgttg cctgaactgg tctcaagctc ctgagctcaa gcaaacttcc cacctcggcc
35161 tcccaaagtt ctgggattac aggcgtgaac tacccgcacc ctgccctcca taactgattc
35221 ttttttttca gacttcactc tgttggccag gctggagtac agtggcgtga tctctgctca
35281 ctgcaacctc cgtctcccag gctcaagcag ttctcgtgcc tcagcctctc aagtagctgg
35341 gattacaggc atgagccaac acgcccggct aatgtttgta tttttagtag agacagggtt
35401 tcaccatgtt ggccaggctg gtcttgaact cctgatctca ggtaatctgc ccacctcggc
35461 ctcccaaagt gctaggatta aaggcgtgag ccaccacgcc cagccatagc tgattcttta
35521 tcagtctgcc tttgggaagt ttccactggg aaacactctc caggactgtt tcggaccagc
35581 cccactttcc aaacaaagaa ttccagtgat gagattaaag cttgtcacaa tatgtttttc
35641 aaatgactgg tagaagcaga ccttaagatt aaaaaagaag aagaaaaaat ggctgaaatc
35701 ttgaatcctt tattgcttta aggcacagaa taggaaatgg atggcgcgtc gggaatggat
35761 gcaggtcagg gacagaccat cactcatgcc accttgctgt gtttatgaaa cagagtgcta
35821 gaagttaaat ttgggggatg cacaaagaga tgcctaatca gatgaactca gagttggaat 35881 tgctgtttgt gagacagtta catcaaagga agaaaagaag gagagaagaa aggtgactat
35941 caggagaatt ttccagccag aagaaagaca cagaggcaca ggcatttcat ggcctgaaaa
36001 aggaagagca cagagacaag tgtggctcca gttaaaggac cagcggagat gaaagatgag
36061 gtcagtgatt tgggtagggg acatagcagt taagatattg ttggtcaagg ctgggcgtgg
36121 tggctcacgc ctgttaatcc cagcactttg gcaggccaag gcgggtgaat cacgaggtta
36181 ggagatcaag agcatcctgg ctaacacagt gaaactccgt ctctactaaa aatacaaaaa
36241 aattagccgg gcgtggtggc gggcgcctgt agtcccagct acttgggagg ctgaggcagg
36301 agaatggtgc aaacccagga ggcggagctt gcagtgagcc aaggttgcac catagcactc
36361 caacctgggt gacagagtga gacccagcct caaaatacta ctactactaa taataataat
36421 aataaaaaca aaacaaacta aaacactcag gagagattag tggtctaaaa gcatattttt
36481 acaatgtatt cagggttatt ccattgtttc ttaaaaacta cctttgggag catctcccca
36541 aacttagttt ggaagtgttt tttctgggtt gcaataaaca agcaaacaaa aaactacctt
36601 ttaattaaaa gtaatcctta aatagagcta acaataaaat caaacacaca aaaaaattat
36661 ccattttaaa atttttttta tatgaagtct tgctctgttg cccacccagg ctggagtgca
36721 atggtgccat ctcggctcac tgcaaccttc accccccggg ttcaagcaat tctctcacct
36781 ctgcctccca agtagctggg actacagtca cgtgcgagca cacccagctc atttttgtat
36841 ttttagtaga gacagggttt cacaatgttg gccaggttgg tcttgaactc ctgacttcaa
36901 gtgatcccct gcctcagcct cccgaggtgc tgtgattaca ggcgtgagac accgcacccg
36961 gccaaaattc atcYaWtaat tgtaaagtaa atttcccttc tgccccagat cacagttacc
37021 tgtctctatt cccaaaataa atggctgtgt agaatttctg atgtgtcctt caatatatat
37081 tctctgcRtc tacatacact taccttcccc taccttttta tgtaaatggg tgtttattat
37141 tcacatactc cgtattaaaa ttgtgattta ttaacataat tttactgaca ttttaatttg
37201 aatggagata aatttataaa ggggattgat gattaagatt ttatgtttaa atattcggaa
37261 gcaaacagta atgcatctta tcttctgagt aggatgtggg ttgaaactgc aaaggcaagc
37321 a-tgagatgtt tcattaacac aaagccttga gtgctccaaa cccaaaagtc aaagcaactt
37381 atcaataaat gccaaaggat gctattctga catatctgtg tgctgtggtt tgaatgcaga
37441 ctccaaatct catgtgtggg aaacttaatt tccaatgtgg gactattgas aggtggggcc
37501 tttaagaggt gattgaatca tgagggctct gccttcatga atgaattaat ccattcgtgg
37561 attaatgggt tgatgggtta atggcttatc atggaagggg agctggtggc tttataagaa
37621 gagaagagag acctgagcca acatgctcag cctccttcat gagatgccct ctgcggcatc
37681 aggatgctgc agagaccccc aaccaggtaa gaaggctttt accaggcatg ccccctccac
37741 tttgggcttc ccagcctcca taactgtgag aaattcattt tctttataaa ctacccagtt
37801 tcagatattt tgttgtaagc aacagacaac ggactaagac actgtgttat ctaattatgt
37861 gattcttgtt ttactattta tttatttagc cacttgtcag aaaaattttt ttttttttga
37921 gataaagtct cactctgtca cccagactgg agtgcagtgc tgctctcatg gctcactgct
37981 gcctcgaact ccctggctca aacgatcctc ccaccttagt ttttcaagta gctgggacta
38041 ctacaggtgt gcgccaccat gactggctac tacttttttt tttttaatat tgcccaggct
38101 ggtctcaaac tcctggcctc aaaagatcct cttgcctcag cctcccaaag tgctggcatt
38161 gtaggtgtga gccaccacac ccagcccacc aaaagttttt tcagacatgt acctgagtga
38221 ttaagagttt aggattggct gggcacggtg gctcacgcct ataatcccag cagtttggga
38281 ggccaagacg ggcagatcct gaggtcagga gttcaagacc agcctgaaca acatggagaa
38341 accccatctc tactaaaaat acaaaatttg ccaggtgtgg tggcacatgc ctgtaatccc
38401 agctacttgg gaagtctgag gcaggagaat cgcttgaacc cgcaagggag aggttgcggt
38461 gagccgagat cgtgccattg cactccagcc tgggcaacag gcaacaagag taaaactctg
38521 tctcaaaaaa aaaaaaaaaa aaagagttta ggatctatat tcgctctgcc tgggtccaaa
38581 tcctggatgt tattaactgt atgactttgg gcaagtttat taacacctcc attttaaaaa
38641 aatctgtact aggaatataa taataaatgc ataccctctc aggatttgat taaatggaat
38701 aaaatatata gaattcctag aacagtgcca ggagtttgag gccagcctgg gcaacatagt
38761 gagaccccat ctgtacaaaa aatacataaa aaatgagggg ggaatagcgg tgcacacctg
38821 tggactcagc tacttgatag gctgagatgg gaggattgct tgagcccggg ggccaaagct
38881 gcagcagtgc agtgactgcg tcccagtaat cccagcactt tgggagggca aggcaggagg
38941 atcccttgag gccaggagtt caagaccagc ctgtgcaaca taaagagaMY ccatctgtac
39001 aacaacaaca aaaattctta cgcacttagt atttgccaga tataatgcca atttctaggg
39061 cacaaaggca aatggtaact acctccctgg attgttgtaa aaataaagca tgcaaaacat
39121 tttgtgcccc caatgatccc ttcctcatgg tgtccccaca cttttatttt gtgctgtcac
39181 aatgagcagg gcaccaccag gatactggga aataatgacg tgtgatttcc aagaccaggt
39241 cacaaaaaac attttgcctt gccctcttga atcacctgct ctggtggaaa ccagacaact
39301 gttatttatg tcatagcatt caagcggccc tatggagagg tccatgtggt aaggaactga
39361 ggcttcctgc caacagccaa gtgaaagagc catcttggaa gaggatccgc ctcccaagtt
39421 ggccaagcca acattttgag tataacctca taagagtctc tgagccagaa ccacacagct
39481 aagctgcagt aactttgaag taataatgat ttattgttgt taagccacta agttttggga
39541 taatttgtta tgtagtaata gatagctaat acagttgtgc ttattacgtt tggtactcgt
39601 tactatcacc accacatcag tgcattgtag aaaacaaatt ctatgtatta aactgttttg
39661 tttcccctct ggattaagct gggctcacaa ggggaaaggt gcccaccaga gtttttttct
39721 tttttatgta atcaagtttt taaaatttta ttctattcta ctttattttt ttaagttcca
39781 gggtacatgt tcaggatatg caggtttgtt atgtaagtaa acatgtgcca tgatggtttg 39841 ctgcaactat caactcatca cctaagtatt aagcccaata tgcattagct ctttttcata
39901 acgctctacc tccctcctac cctcccgtaa aaggccccag tgtgtgttgt tcccctccct
39961 gtatccatgt gttctcattg ttaagctctc cacttataag tgagaacatc tggtgtctgg
40021 ttttctgttc ctgcattagt ttgctgagga taatggcttc cagcttatgg atgtatagta
40081 ttccatggtg tgtatgtacc agattttctt ttttcttttc tttttttttt tttttttttg
40141 agacggagtc tcactctgtc gcccagctgg agtacagtgg catgatctct gctcactgca
40201 acctccgcct cccgggttca agcgattctc ctgcctcagc ctcccgagta gctgggatta
40261 caggcgcata ccaccacgcc tggctagttt tttgtgtttt tagtagagac ggggtttcac
40321 cgtattagcc aggatggtct caatctcctt acctcaggtg atctgcccgc ctcggcctcc
40381 caaagtgttg ggattacagg cgtgagtcac tgcgcctggt cagtaccaca ttttctttaa
40441 ccagcctgtc gatggaaatt tgggttgatt ccatgtcttt gctactgtga ctagtgctgc
40501 aatgaacata cacaaaatta tctgaatgat tgcagtaaac atcatatatt tgtttgccag
40561 ctaccataat aaaataccat agattgggtg gcttaaccaa cagaaattta ttttcccaca
40621 attctggagg ctggaagtcc aagatcaagg tgttggcagg gttggtttct tctgaggcct
40681 tggcttgcaa gatggccacc ttcttgctat atctttacat gatcattcct ctgtgcgtgt
40741 tcgtgtccta attttttctt cttataagga caccagtcat tttggattag ggctctttca
40801 tatgacttca ttttaatctt aactgcttct ttaaaagccc tatctggaaa taaagtcaca
40861 ttctgaggtt actgggggat agaattacaa caaatgaatt ttgggaggac acaaatcagc
40921 ccataacaca tcattgccag catcacatgt ggagcattca actggagaaa ctgccctaat
40981 ttctcatttg aaggtctaaa catctctttt atcaaaataa gccaggttct attaagaagg
41041 ctgaatgtgc atctggctcg atcaacaggc gtctggctgc ttcaaatgct tgattttttt
41101 cattctcaga gaatgaaatc aatgttgaaa tattgagtat attctataca aaatcttcat
41161 gtcacaatga ataagaaaag tagttttttt tgagacagga tcttgctctg tagctcaggc
41221 tgcagttttt ggagtgcact ggtatgacat aactcgctgt agcttccatt tcttgggttc
41281 aagtaatcct cccacctcag cctcctgagt agctgggact ataggcatgt gctaccactc
41341 caggttaatt attattatta ttatttttga gacggagtct cgctctgttg cccagcctgg
41401 agtgcagtgg cgccatctct gctcactgca agctccgtct cccgtgttca cgccattctc
41461 ctgcctcagc ctcccaagta gctgggacta tagacgccca ccaccacacc tggctaattt
41521 ttcgtatttt tttagtattt ttttgtattt ttttaataga gatggggttt caccatgtta
41581 gccaggatgg tctcagtctc ctgacctcgt gatccacccg cctcagcctc ccaaagtgct
41641 gggattacag gcgtgagcca ccgaatccag ccactccagg ctaattattt tattgtattt
41701 ttagtagaga tggggtcttg ctatgttg.cc caggctggaa tcaaactcct gggcttaagc
41761 aatcctcctg ccccagcctc ccaaagtgtt gggattatag gcaccagcca ctgcacccag
41821 tcaagaaaat attttctaat actgaataca gctaaagaat ggatggtaaa ccattattgt
41881 ccagtcagga tcactgtcat ccaagtggga cactcagtat ttgagattct gggtggtgct
419'41 ataatacttg ttgccttggt atctgagtga gcacttagtc taagtataat aattttctgg
42001 , aggagtatat tagggttctc cagaaagaca gaaccaatag aatggatgaa tagatagatg
42061 gatagataga tagaaagata gattgatagg gccgggcgcg gtggctcaca cgtgtaatcc
42121 caccactttg ggaggccaag gcaggcagat cacgaggtca ggagatccag accatcctgg
42181 ctaatgtggt gaaaccccat ctctactaaa aatacaaaaa actaaccaga cgtggtggca
42241 ggcacctgta gtcccagcta ctcgggagct gaagcaggag aatggcgtga acccgggaag
42301 cagaacttgc agtgagccga gatcacgcca ctgcactcca gcctgcgaca gaacgagact
42361 ccgtctcaaa aaaaaaaaaa aaagaaagat agattgatag atagatatgt gagtatgtga
42421 ggggatttgt taggggaact gtctcatgca attatgaagg ctaagaagtt ccacagcagg
42481 ccatctgcaa gctggagatc ctgggatgct gacagcatgg ctcactctga gtccaaaagc
42541 ctcagaaota gcggtgtaat tctcagtcca gtgctgaagg cctgagaagc tagggagtga
42601 gggtgcactg ctagtgcaag ttgtggagtc caaagaccag agagccttga gttgtgacat
42661 ctaagtacag gagaggaaga gtgtatctca gctccaaaag gtagatcaac acattcacct
42721 ttcctctgtt tttgttattt tcagtactgg cctttggatg gcacccacct acatggaggg
42781 cagatcttcc ccacctagtc cactcacact catgcgttaa tctcttctgg aaacacagac
42841 acacccaaag atagtacttt accaggtttt taggtattct ttaatccagt caagatgaca
42901 cctaaaatta accatcgcaa gggtatattc aatctgaatg gccaaaatta tctgatagag
42961 ccttcagata cttgtactta acagatgtac tcttgggatt aaatgagttt atgctcaaga
43021 aatgatatta gccaggcata ggggtgcatg ccgttgtcct agctaccgtg gaggctagga
43081 caggaggagc attgcttgag actgggagct tgaggctgca gtgagctgcg atggttccac
43141 tgcactccag cctgggtgac tgggcaagac cccactgcaa agagagagag gcagagagag
43201 agagtagaaa tggtatctgt tattttacat taaaagttct ggaatagggg agtgctatgg
43261 tctgaacgta tccctccaaa gttcatgttg gaacttatga cccaatgtga tagtattaag
43321 aggtggggct tttacgagat gactaggcta ttagaggcct acccttatga tgaatgaatt
43381 aatgtcctta tcaaaaagat tccatttcca ctatgctgag agcggaaaac aaacaaacaa
43441 acaaacacaa atgaggttcc acacagcact catccttttt gcccttccac ccttctgcca
43501 tgtgaggaca aagcagcaag tctccatttt ggaagcagag agcagccctc accaaatctg
43561 aatctgttgg tgccttgata ttgaacttcc tagcctctag aactgtaaga aatgtttctg
43621 ttttgttgtt gttttttccg agacgaagtc tcgctcttgt tgcccaggct ggagtgcaat
43681 ggtgtgatct tggctcactg caacctccgc ctcccaggtt caagggactc tcctgcctca
43741 gcctcctgag tagctgggat tacaggcacg tgccaccacg cctggccaat ttttgtactt 43801 ttagcaaaag tggggcctca ccatgttggc caggctggtc tcaaactcct gacctcaggc
43861 gatctgccca cctcggcctc ccaaagcact ggaattgcag gcacgaacca ccgctcccag
43921 ctgagatttt ctgttctttg taaattaccc agtctcaagt tattttgtta tagcagcaaa
43981 agcaaattaa agaaggtata atatcaggct ctcttctgat tccctaggct ctgctgcacg
44041 aattgttatt tccagaagtt cccataggaa gttctcagga aatgaaatgg atatgaggta
44101 acctcatgtc ggggagtcag tctatggagg atcataatca tacaacaaca tggtccaccc
44161 ttgctataat ctattttttt tttttttttt tgagacggag ttgctctgtc acccacgctg
44221 gaattcaatg gcgtgatcta cgctcactgc aacctctgcc tcccaggttc aagagagtct
44281 cctgtctcag cctcctgagt agctgggacc acaggcacac aacaccacac ctggctaatt
44341 ttttggattt ttggtagaga ctgggtttcg ccatgttgcc caggctggtc ttgaactcct
44401 gagctcaggc aatttaccta ccttgccctc ccaaagtgtt gggattacag tcgtgagcca
44461 ctgcacccag ctgttttctg ttttctgact tttatttttg agacagggtc tcgctctgtt
44521 gcccaggctg gagtgcaatg gtacaatctc ggctcactgc aacatctgcc tcccgggttc
44581 aagtgattct cctgtctcag cctccacagt agctaggatt acaggcgcct gccaccacac
44641 ccagctaatt tttgtatttt tagtgaagac atggttttgc catgttggcc aagctgttct
44701 caaactcttg acttcaggtg atccgcccac cttagcctac caaagtgctg ggattacagg
44761 cacgagccac tgcgcccggc cttgttttct gttttgtttg tttgtttttt aagagatagg
44821 gtcttgctct gtcagctagg ctggagtgca gtggcacaat cagctcactg cagccttgaa
44881 ctcctgggcc caagaaatcc tcccaccaca gccttctgag tagccaggac tataggtgag
44941 cactggttaa atttttaatt tttttttgta gagacagagt cttgctatgt agtataggct
45001 ggtctcaaat tcctagcctc aagtggtcct ccagcttcag cctcccaaaa tttgggaatt
45061 tcaggcatga gccactgcat ctgaccgtac aaaatgttaa taaactggcc aggtgtggtg
45121 gctcatgcct gtaatcccag tactttggga ggctgaggca gggagatcac ctgaggtcag
45181 gagtttgaga ccagcctggc caatgtggtg aaacccccat ctctacagaa aatacaaaaa
45241 ttagccaggc gtggtggccc atgcgtgtaa tccgagctac ttgggaggct gaggcaggag
45301 aatcgcttga acctgggagg cggaggttgc agtgagccga gatcagagca ctgcactcca
45361 gcctgggtga cagagtgaga ctccgtctca aaaaaaaaaa aagttaataa accttttact
45421 cctggcctca agtgttccgg ctgcctcagc ctcccaaaat gctgggatta caagcatgag
45481 ccatttattt ttcttttttt gagacgggag ctcactctgt tacccaggct ggagtgtagt
45541 agcatgatca caactcactg caaccttgaa ctcttggatt caagtgatcc tcatgcctca
45601 gcctcctgag tagctgggac tataggtgca cactgacatg ctcagctcat tcaaaaattt
45661 tttcataaga gatggagggg gggtcttgct atgctgccca gcctggtctt gaactcctgg
45721 actcaagcga tcctcttgcc ttggcttccc agaatgctag gattacaggt gtgagctact
45781 gtgcctggct tttgattttt agacagggtc ttgctctgtt gccgaggctg gagtgcattg
45841 gtgtgatcat agctcactgc agccttgaat tcctgagttt aagtgatcct cctgcttcag
45901 tctcccaagt tgctaggact acaggtgtgt gccaccacgc ccagctactt tatgaacgtt
45961 ttgattccaa agtctgaaaa aagttttcca gattcatctc caggtcaaat aatgtccctg
46021 ctgtcaggca tcctgcttta gaagcaagat gtagcatttt tcttccttta cttgcagcag
46081 gcagctaata actgttatcc tgtgctggac catccatgtt ctacgcattc taacatgtgc
46141 tttacataaa aaattagccc aagtgaccct cactgtgaca ttacacggta cgtattatta
46201 atcttttttt tttttttttt ttttttgagg tggggtctca ctttgttgcc aaggctggag
46261 tacaatggct catgcctgta atcccagcac tttgggaggt tgagttggga agatcccttg
46321 aacccaggac ttcgagacta gcctgggcaa cataggaaga cccccatctc tagaaaaaat
46381 aaaaacatag ccatgcatgg tggcatgtgc ctgtggtccc agctacttgg gaggctgaga
46441 aaggaggatc acttgagcct gggaggttga ggctgcagtg agccatgatc atgctactac
46501 actccagcct gggtgacaga gtgggaccct atctctaaaa agagagagag agagaaagag
46561 ggagagcttt taaaaaatgg caaaatgtgc ccacagctga tgggcctatt tttctttttt
46621 ttttttttat tgagacggag tcttgctctg tcacccaggc tggagtagtg gtgtgatctc
46681 ggctcactgc aacttctgct tcccgggctt aagcgattcc cctgcctcag tctcctgagt
46741 agctggaaat acaggcaccc gccaccatgc ctggctaatt tttgtatttt tagtagagac
46801 agggtttcac cacattggcc aggctggtct cgaactcctg acctcaggtg atccgcctgc
46861 ctoggcgtcc caaagtgctg ggattacagg cgtgagccac tgcacccagc caatttctat
46921 ttcttataga gcttacggta ggaggcttga agatgggaac agaagaaaaa aggttgaagg
46981 gcccagctgc tgtgtgttgc aagacaggaa attgctgaca gtctgcaatg ccagcagcct
47041 catgggtggt tatcaccagg acagaggcct cagaacaggc aggactgggt gtgctggcac
47101 tcacggctac gtgcagatag agcccgcccc tgggccttgg tgtgtgacct tcttcccagg
47161 tgcttctttg ggaagctgta cagtgaggga gatgaggcag actaagggca ggacacacta
47221 gcataggagc ggtggcagct aagtaggctg actgcccagg gattgcagag ttcacctgca
47281 cagtatgaca tctctcatcc ttttggatag attgttctat aaggtggcct ccacctaccc
47341 ccaactccca aatctgacat tcattcatct acttcccctg aagtgctccc tagcacttgc
47401 catgagtttg ttcactcttg ggaccccaaa tccaaggcat ctcactggct gagattcagt
47461 ttaccacctc attgatgggg ggactcttat gtcacagaag taattttctc actgatagga
47521 cccaagatgt cgtcctattt ttaatgtggg tacaaatact aagtaaatat gatcttagtg
47581 ttttgccaaa aggaattaag atgtttttaa tgggtcacaa attcagaaag gggttggacc
47641 tggtgggaca ggttggtgag gcccagaagc tccacttcaa gtgcagtact ggacatcccc
47701 ttccagtacg ggaggtctga cttgtgcagt caagcccaat actggcacag ccacaggcca 47761 gggatggagg cttatgagtc cactggaaaa cagggaggac tgagtccagg gtctcaaggg
47821 tttagtagtg agggctcctg tgaagaaccc ttcactcagt ggtgttcatt accggcccct
47881 tccttagtat gttccaggcc ctgaggatgt accttgcatg ctctgaccct ttgaacacca
47941 aagaaataag caaggagRta acagatgccc caaagagatg aatggtgtca tgagatctgg
48001 gggctgggac cttgtccagc ctaaaggtgt aagtaccatg gatgagcagt gtcttattta
48061 atcagcaacY agagatttcc tgaggtttaa ctgggaaagc aggttcatat actacaagac
48121 accaggtgct tcacattgct tctctttatt ttcaacagtt tctttacaac agtctacaca
48181 gggattaact cctacatgta cttcccaagc cagaatctcc ctttagaaat tcagattcta
48241 tttctaactc tataagatgt atctccccaa agaKcacatt aactcctcaa gtcaacatct
48301 gcctaYcccc aacttcccct ttttttcctc actgaacatt tgtctgaatg ttttttcctc
48361 actgaacatg ttttcctcac tcacactgaa catttgtctg aaactgtggc tgctttgttg
48421 cttcaagatg catgcacatc ctggctttag tgtccaagta tgcagaatga agcatttgat
48481 atgtgcaccc agctaRttag gcatgaaaca ggggcacagg atgggctccg ggtcatagaa
48541 ggttctgtcc ccacagtgag gacaggggtt ggcactaagc cagaccatgc tgggccgccc
48601 caactcacac agcaactccc tgagcctggc atgcagatag gcaagcctct ccaggtggag
48661 ggtaccatgg atgtcctcat aactctccag ggggacagga tacagcacgt gggtcagatt
48721 gctcagcccg atgaggtgct gcaggagact ctgcaaggcR gatatggaga tggaattccc
48781 gtagaagctt aaggttgtaa gctgggagca gtggctcagg gaaggcagga gggcaaggag
48841 ctgatcatcc gtgatcccac actcatcaaa gaccaggtcc tggagggtgg cagaggctct
48901 ctccagcaga gcttggaggg gctcgggact tacatcggtc agcatgaccc cacttagact
48961 caggacactt agctgactga cgctgggact ctgggacaga tgcatcacat ccccttccga
49021 aagccggcag ttagttattg agagggtttc caaggggttc atcacgtgcc tgcaaataga
49081 caaaKcagtt agtgctgggg aatggtggta gtggggtggg gagactggag agaggcccat
49141 ttcaccaaaa cccaaagtct tcctgatgat ggttaaccgc caggatgcca tgctgtaaMg
49201 gagcattcaa ggagtacaag ttcaggttta gtcctttctc catcatttgc tgtgtaagtg
49261 ggtcaagtaa acaaagatct caaacactcc cttggctSat ctgtgaggca gRgtccaaca
49321 cagacctctt aggtggtgtg agcatgaatt gaattgagag cctgatttga tgtctcaagc
49381 aaggaaaggc atcagtgaat tttagtattt gaagatacaa ctgaaaatac tttctttcaa
49441 ctcgggcttc cttctgccac tgcctgggcc ccagtcacct ctatctctgt gcctgatgaa
49501 gctactaggg agtattcaca gtggacaaac tggggcaagg tcagcaacat ccaaaggggg
49561 ttcccaggct gcagggctac cttaggtcac tgtatcatca gcaaaccacc tatcaatttt
49621 tgcaatttct ctgttgttcc ttctcccaca tctccagaat cctgcatttc ccacctatta
49681 ccctaactgg aattctaaat caccctttac agacaggaaa tttgaggcag gtttagaggg
49741 agcagctcat gttgagaagc tggtgagcgc acagcttaca atttcaaatc tcacctttga
49801 tgggctttcc cttcttcaat gccctccttg cttacttttg atcatcttag gcatcacttc
49861 ctaaggaaga atgcctaaag ccacccttgc caggccccaa cctcccatta cagagttttc
49921 taacaaggag tcactgttaa tagcatctat cctaatgtat acctctaacc cttatgagta
49981 gtagttatgt gRtaccatgc ttggggacag tggtgaacaa gacgtgttaa ctggtagtga
50041 cttgctcact cttgcattat tggtggctgg cacatacaag atatccttta ttgtttactg
50101 caataaggaa gggcttgctc tggtctgcag agaaatctca ccatccctca cctgagcaac
50161 tgatccaggc ggcctctaag gaaaaataaa gagtccacat agagagcctg caggcactgc
50221 agactgagga actgagaggt gaactgggcg atatactgct cttccttctc cggggaaatg
50281 taggaagatg catggatgtg ggagaggagg agtctacgca gattaatcat ctggcccaRg
50341 taaggagaaa atttcgccaa ggtgggtagc ttccaggtac aagtcacttc caaatcttca
50401 atagagtcca gctgcaccat tttcaggatc atcttgatat cctgcatggg cattgcaaaa
50461 atcttcagct tcttacagca caggcgtagt acatttttct ttcgcttcac tttctcaatg
50521 aggtaggaga acaattcatc acaggcacct tccttgagga acaggtctac gagcacctct
50581 actggaatga agggctgctc tgcctctgtg ctcaaaccat ctacttttcg cttctttgtc
50641 atgggctgag ctgcttctgg ctctggaaat gagtacagac tggccctgtt tccagaccat
50701 acagtccaga agtcctgatg agagttcttc cgtaaatcca gcacttgaag tttccacctc
50761 ctgtggggaa aaacaggtaa ttagctgaga tgatgcttta agatcaactc aaaataagac
50821 catgagtctc ataatgtagg taagaaccaa acagacatcc accttagatc tgcactttta
50881 cttcgtactt caggcatcag ctgctccctt tcccactagg atcctgggat ccttcctggt
50941 ccccaattcc caccccacca ggaggaagca ggtgcatgtt ccttcagaca cctctgcatt
51001 ttaatcagtg ccccatgtcc agaagcccat tccaagagtg acccctgcag tagccccaag
51061 gcctccctga acttccttgc tggcaaccat cagagcctct ggcccagcct taggcgctcc
51121 atgctccctg accccagctg catcctgctc aggttcccag ggccccacac caagctgcta
51181 ggtcaccctt acctggggcg aacctcctgg gcaaggagca catcaagtcc atcaagcaca
51241 gctttgaagg tctccaggtg aagatgttgt cccttcatca gcactcccag agggaggcag
51301 gtgaagggcc aggcctgcac cattgccttc agggtctggc tgtgtctccc gtcaaaggct
51361 gccatgaaga gtggcgggaa gagctccctg ggcagcaact ccagggcggc aatggccagg
51421 gcctcatcct tcagcaggct ctgccctgcc agctccacaa gtctccgtgg gcttgtccac
51481 acactcatgc tgatgtatcg gctctgaatg gaaccctgag gaaacataca gggaacaagg
51541 catccctttc agcccaagca taagcaactc tatcttttcc tcacccttct gagggaccaa
51601 cttagggcca aagtcactgt gctagcaaca gcaggggagt tctcagttta ccccgattcc
51661 actctgcact cggtggccac aaagccacag ctctgctggc accaggatga acatctcatc 51721 aagtgtagag gaggggacag gacggccact ggcaccatct tgtatctacc cactactcta
51781 tttaattcta gtccccctgg gaagtgagaa ctaaggagcc tgagggctga ccctgctcat
51841 tttgggaaaa agtttctgat gatcactcaa aggcaattca aatgggagtg tcacatagcc
51901 caggacagca tccttctctg ctccaacaaa taagccagat gggaaagatg aggaacacac
51961 acacaatgca caatggatgc cattatgttt cactaaaaaa ttttaaatga gaaactacag
52021 aagcagcaca acagtattac atagcaattg gaagtacaat aaaaaaaaaa cattctgaga
52081 catgtatttt aagtgcgtct cttttacgta tattaagagt tactactttg acatggaaat
52141 cagcggagca aacacttcac agtaaaacaa agcctggttc ttttctccca aaacattagt
52201 tttaaaatgg agaaataaaa attgtgtata tttatggtgt acaatatgat gctctgaaat
52261 atgtatacat tgtagaatag gtaccttaag ctaattaaca tacatatgat ctcacatatg
52321 tgtggtgaga acacttgaaa tatactctta gcaattttca tgtatacaat acattattaa
52381 ctacagttac catcttgtat actagatatc ttgaaaaagc ctccaccctc cagcacagtg
52441 gctcatgtct gtaatcccag cattttggga ggccagggtg ggagaatggt ttgagcccag
52501 gagttcaaga ccagcctggg caacacaagg agacctcatc tctattaaaa aaaaaaaaaa
52561 gaaaaaaaag aaagaaaatg tctttaattc caaagcatct gaaaagaaaa agaaaaaaat
52621 ctatggaata ttttttaacc ttttagttaa aatggaatat tttttaacct tgtagttaaa
52681 atggaatatt ttttaacctt gtagttaaaa aaaagcctaa ctagtaaatc actgatttaa
52741 aaaatcataa atgatgcaaa ttataattgc ttgccacata ctatttaaat tgtaataaag
52801 taaaattcaa agtagatgtc ttttattttc caaaaaaaaa aaaaaagccg tttaagaaat
52861 tatttttaac atatacaaaa tggcagaaat caaaatgttt gggattcaat ataaataata
52921 aatgttccag gtaatggtta tactaattac cataatttga tcattacata ctatatatgc
52981 ataaactgaa atttcacatg taccctataa atatgtacaa ttattgtgta tcaattaaga
53041 aatcaatcca ataaccaaat aaaaatgttt gggattaagg caaaactaca tttagagaca
53101 agaacaaagc ctgttccttt gtaaggaaag aaaaaggaaa accatctaag cagcattggc
53161 cctcatggcc cgtggagacc ctgaggtgac agtagaagga ggcagtggcc "atagcctgag
53221 gctcccgact caccagaccc agctgtggtg cagaagccat ctgcccagac ctctgggaag
53281 accacaacat tgactccatg gcctctgggt ccctcctctg gcccctctga agctattaca
53341 ggcctttctg ggcatacctc ccctttcaac tccacgctgc caacggaaga gccaaatcta
53401 attacattcc tggatctcca gccagtgaat cctgatgggg ttttttactt acttcaggtt
53461 caactgattg aatgggatgt ctatttacaa aaaggaaaga aaaagtgaac aaaaccaccc
53521 agagagtata actgttaggg gaatttttgg ccacatcaaa attacccaaa tagtccagtt
53581 aagatagctt tatgaagagc atcatgacat caccccttcc cactcccaaa caatccccaa
53641 gtatcaaatt gtcacttata ccccatgttc cctaacacag caaatcacat gcctatccaa
53701 ggaaaaaggg aggaacaaca gaggggtggc tggtcttcca gactttgaaa gtcaaggctg
53761 tcctgaagga aggtgggtca aaattacact acaattagga gttagtcaga agtaaggctg
53821 ggcatttgta atgggactga gtggattacc acaaagacag ataggttttt ttgggggcgg
53881 aattgaaaga ctttggcctg gatggagtag agaaattaaa aaggggaatg gctaaggaag
53941 atgaaagata gttccagtaa agccaaggta atcactgagg cagggaggga gttcaatctt
54001 cctccctccc tagagggaaa gggtgttttc tcccagaaag tcaggatcct cttggcaaac
54061 agccttgaca agattaaatc taggaatgtg ggctggagag gtgggataga gagtaatatt
54121 ggtgaccaaa cctaggacag caacattttc aagtctgaaa gaccttgtgg tttcttagga
54181 atcttggagc caggaaagaa ataaacaagg gctgaaagca cacatccctg caattaaaga
54241 agttgtcaga agaccttctc tttatggtta ggtgtcctct tccacactgg ggctgttcac
54301 aaRcagcagg tcaggccttt accacaaaca taatctccaa tggccagcCG cttcctaagt
54361 attactaatg tttccacact tcaagaaaac agctgttctt tcttgcGcct aagctgctca
54421 ggtacagaga ttctggtcag cgagatgcaa tcaggcctat caattagact cagtttttct
54481 ctatttccat gctaggcaca gtgcacaaat acattttccg tcacaaaaaN ttgaagttat
54541 atttggcatt taaggatctg cttagggatg attcctatcc tgggcctgat cacccactgg
54601 tgcttttgaa gttttggaac ctctccaccc aaagaattct ccaggccatt ctctgcctca
54661 tgcactgtga agtccctaca attcttatat tctactggca agtgtccagc tggaatgtct
54721 tgcggcctga ctttgtttac tgtggcctgc gtaaggaggc tgtaattcgt tgaaggatgt
54781 tttccatttt ggttctctct ctggagactt tctcctactt ttcaccctca acacctaatg
54841 gcctttgtga aaacYgggtt aaactacaat ttgttctaca tttcctttaa ttcccaggca
54901 atcatcttca ctcatΥagct gcgggagact gagattagta ataaaggtca ttgtcaatgt
54961 ccctaattat caagcagcta ctgggcacag caaaggccgc agcagctctc attcattacc
55021 tcaaatagcc ttcacagaga atctaaagtt cactgtcatt ttcattttta gtcttgtaag
55081 tccctgactc tgtctgggga agaacctgot cagctccaRg caagcaggaa ttggtagccc
55141 ttatgtgagg agtttagaaa gagtttcatt tccctcccta tcaaacaatc taagtgttag
55201 aaagggctga taagcttcac ataaaccatg gaaaaacagg aaaactgagt aagtccaaca
55261 gttaRggaag cttccatggg atccccgctt ccaggagatc caagggagga ggaggaaccg
55321 cgatcRagtg tgagttgtgt aatctctgag ctgtgcaatc tccctgtgag gcagtgacaσ
55381 tcctctgtcc agcttcacac tgccagtttg tccagtggca ctggaggaag cagcaccagg
55441 ggcacatttg cacaaaggtg ctgtccttcc tcccagtata agggagaagt gaaaagactg
55501 gggcccagca tgactcctag ggtccagaca tgaattgggg agatatttct gcatgtcctg
55561 tctactggtt cttatggtcc ttgtgggctg cacaggccag agtggcctct gagctcatca
55621 ggtgggtggt ctgcaccctc ctctctagtc atagaatccc agtggggacc. cccgaagtat 55681 ctgcaccagg tccttcaggt gttttttttg gagtgcagtg gcgcaatctt ggctcactgc
55741 aacctctgcc tcctgggttc aaacgattct cctgcctcag cctcccaagt agctaggatt
55801 acaagcacac gcccccacac acggctatat ttttgtattt ttagtagaga tggggtttca
55861 ccatgttggc caggctggtc tcaaactcct gacctcaagt gatctgcctg tctaggcctc
55921 ccaaagtgtt gagattacaa gagtgagcca ccatgcccag cctctttttc ttttttagag
55981 acagggtctt actatgtttc ccagactggt cttgaactcc tgggctcaag tgatccaccc
56041 atttcagcct cccaaagtgc tgggattaca ggcttgtgga taccaacagc acccgagcac
56101 ggtcccacag tccaacgcac tgtgccgtcc ttgagccttt agttccagca caagtctccc
56161 ggcttcaacc caggttgcat gttctccctg atctccccag gtggcaccca aggatgaggc
56221 agtgcaatct tgttgctcac ttcccaggac cgacacttac ccctaatggc ccaagcccat
56281 ggggcagtga gatcaaagcc tcctggagct catactctct gacaccactg gaagtgtggg
56341 cttttgcgtc tgattacccc ggggaaaggt tctgaatgta atcgtggctt tggcaataag
56401 gtccacaatg cattaagatt tttttttttt tttggaggag gagtttcact cttgttgccc
56461 aggctagagt gcactggcac catcttggct cactgcaacc tccacctcct gggttcaagc
56521 gattctcctg ctcctgtctc agcctcccaa gtagctggga ctacagatgt gggccaccac
56581 gcccagctaa ttttttttct atttttagta aagacaggat ttcaccattt tggccaggct
56641 agtctcgaac tcctgacctc aggtgatctg cccacctcgg cctcccatgg tactgggatt
56701 gggattacat gcatgagcca ccatgcctgg cctagggtta atttttattt ttggagacgg
56761 agtctcgctc tgtcgccagg ctggagtgca gtggcatgat cttggcttac tgcaacottc
56821 gcctcctggg ttcaagcgat tctcctgcct cagccacttg agtagctggg actacaggtg
56881 catgccacca cgcccagcta actttctgta cttttagtag acgcggggtt tcacagtgtt
56941 agccaggaat ggtctcaatc tcctgacctg gtgatccgcc cgcctcagcc tcccaaagtg
57001 ctgggattac aggcatgagc caccgtgccc gaccctaggg ttgactctca tacaaagaca
57061 gcacgggaag tgaaatgctc ttttgtggaa atgaccagaa acaataaaag cggctcctgc
57121 ctcttcgtct actgtgaggg acctcaggat gcagctccca tctggctcga gtgacaagga
57181 cagaggggaa cagggcttct ctgagcacct cagacagctc aggggacctt cttacccRca
57241 aacgccttcg ttccattttg aagcgactta ggctggcctc aggacctcca acgcttggat
57301 ttctaggtct cagtcacttg ttgccacKca cgtctgagag taataatcaa aatgctccaa
57361 aaagaagRat acatgtataa tatttacgaa gcaacggcct cctgtgaaaa cctccgcRaa
57421 tactgctgag gaaactgaca actggcgact caatcccgcc ctttcagtgc aaatctctgg
57481 ctccaaacct gagcaccagt gtttcctggg ctgaaccagg gggcaggagg aacaacagac
57541 catgtggttg gtcacaagtg acccctgtgg tcaaggaccc tgactacggt cttgcagcga
57601 catttctgcc tctgctcccg ccttcctagg acgcctacgc cactgcctgg gccgtcccca
57661 acccccagca ccttcagagg ggccgagtcc cagagcatcc accgcattac aaatgcgcac
57721 ccaccctgga gagctccaac cacgagcacc gccgcctcgg ttcaaggcat ccttacagcc
57781 acaactcctc aactggggcc aattttccgg ggcaggcggc ccggggtgac ccaagtggcg
57841 cacattattt tctttggtct tcaacccatt tccagagaga aaacagggac ccgggacagg
57901 ggtggtgccg ggggcacaag cccaaggtca ccctgcgggg aagcggagga atcagggctc 57961 gaacttacgt ttttcctcag agagttcacc acaccgcgaa gttgctggaa ggaaagaacg 58021 agacaatggc tcagctaagc gccctagccg ctgcgtgggg cggggggggc ggcggtgggg 58081 gccggggggc tgggcagcct ggtctgacca atccccgttt ctcagtcctt ccagggcaaa 58141 atctcacgag atccagtgag gtcgcccaga gagtcgagag gggcttgttt tcaaagactg 58201 ggaggaggag gggcagtggg ggaggggcag tggggggagg agcagaggag aagggagcag 58261 gaggagggga aaaggggaga ggaagttgga gagcagagga ggctgggggt ggggagcaga 58321 tgggggtggg gaaggagggg agatgggggt ggggaagggg ggcgggagga gggttaggaa 58381 tgggggaagg aagacagggt gggggagggg tgggcatggg ggaaggaaga cagggtgggg 58441 gaggggtggg catggggaaa gggagacagg gtgggggagg ggtgggcatg ggggaaggga 58501 gacagggtgg gggaggggtg ggcatgcggg aagggagaca gggtggggaa ccgagaagaa 58561 agacagtggg gggggatgga ggaaggaaca cagggtgggg tggggggtcg ggatggggga 58621 agggaaacaa agagtggtgg aggcgtgggg attaggaacg gagacagagg gtgggggagg 58681 ggtggggtgg gggaagggag acagggtggg ggaggggtga ggatggcgga aggtagacaa 58741 ggcgggggag gggtggggat gggggaaggg agacagaagg gggggagggg tggggatggg 58801 ggaagggaga cagaaggcgg gccagggcgg gaatggggga agggaaacag ggtgggggtg 58861 gggatgagag aagacagagg ttgggggagg ggtggggatg tgtaagaaaa acaaagggtt
58921 ggggaggggg agggaaaaga ggcagagagt gggggagggg gtggggatgt ggaaagaaag 58981 tcagaaggtg gggggtgagg ggtggggatg ggtgaatacg tgatggctgc ggcctttccc 59041 accagacagt agggtggggg cagggtggaa aaagggggtg cggtgggcga atggggaggc 59101 ggggggctga ctgggagtgt gggacggggt gttctgggat cacggagacg tccaagtctg
59161 gactctgtcc tgggtctgtg cttctggcga cctccctttt cttcagtggg atgcggggtg
59221 caggggccgg ttccaggtcc cctgagctca agtctcgccc caccccgccc cgcaagtcta
59281 gaaaagatgc ccctgccctt ggctgggtaa tctctggatt tacctactgt ctgtaacccc
59341 ccgggctgaa gagaccaccc cccgggagtc aattaactta ctctctggag cgcgcgttcc
59401 tccctgctcc caggagtcgg tttcccaaaa ctttctgagg cccgcgcatg ctcccctcga
59461 ctccccgtgt ttccactctc cacagaaatc cacgcattca cgcccctccc ctcccccgag
59521 cctgcagagg actccgccct gctttcccta cattcagggc tgctcctttt gtcgccaata
59581 caaacctgtt gacaggtcac gcctgggaag cgggtggggt gtcccggagc ggtgctgagg 59641 cgctgcaggc ccggcttctg gctgcggggg agctgtaccc tgaagcctcg ccggaactcg 59701 cgtctggggc cagcaggggg cactagagtc aacagggact gtatgcaaaa cccacttcct 59761 cccaggccct ctagggggat ggtcaggctt ctgcagagat ggggaggatc ctttcctggt 59821 aaggggagag ggacgggcta ggagccaagc ggaaggaccc cgtgttcaag gcccttcaag 59881 ggacgggaca ggcgaggaat ctctttgagt cttttgaatt gttttatcag tcaggcctgg 59941 gaagtactcc gcctccacaa actctgtttt cctgaaaggg gtcggggggg ggaccccaac 60001 atctcctgcc acaggctatg atgggcatgg tggctaagag caacaggccg cgtggtgagg 60061 ctggcttttt gtggctctgc cctgccattc cccgaggcat ccccatgcca gtgaacagag 60121 ctgcgggttc ccagctgcac agatgaatgc acgaacaaaa cataggtcca tRcaaaggaa 60181 gtcattgcat tgatccagat tcgacctggg attgtcgcat aaaacagggc tgataaaaac 60241 cgtYgtgttt tcaccYtSga aacagagact cagcctcccc ctctYtgctt gaggcattca 60301 gcaaaggttY gtccttgagt ggctgtggat gctcaaggac ttacgatttc tgactttata 60361 aagtttaggg tgaaaacaaa aaacaaaata aggagaccat tgttttaaag tggccgttgt 60421 agctaattat ctcaagaaag caaacaRgct ggtactgaaa gttacacagg cgggcggctc 60481 cctgggaggg ttgcccagct ctccttttct gagacctgag cagggagcag ccagaggagc 60541 ttgaagggat ggtgttcagg gcatgggaac aggctgcaca catcttcacg gagtacggtg 60601 cttgtccagt ttgagaaggg aaagaaacgg gagcagaggg ctgatgagaa ggcagcagat 60661 ggatagtgcg ggattgaaaa ggggatccat ttgaagactg gccaccatta tttattttca 60721 tcatctgatt gctgttgctc cattgagatc gatgcgtgga gaacaagttg gggagtggaa 60781 caaatggcag ggaggggaga gaagtgagtg aacatgaccc tggtccagcc tcataggaca 60841 gagcgtggtc atgaagtcca cagaggagga caggtaggga ggaatcatgg catggccagg 60901 ttgtagggca agaggcagag aggagagagg attgtggagg atgcctgcgg agtgcYgact 60961 gtcagtcgag gctggtcctt tatttattta tttwtttttt ttgagacgga gtctcgctct 61021 tgcccaggct ggagtgcagt ggcgcgatct cggctcactg caagctccgc ctcccgggtt -61081 catgccattc tctcgcctca gcctcccaag tactgggact acaggtgccc gctaccatgc 61141 ctggctaatt tttttgtttt taatagagac agggtttcac gtgttagcca ggatggtctt 61201 gatctcctga actcatgatc cgcccacctc ggcctcccaa agtgctggga ttgcaggcat 61261 cagccactgt gcctggccaa ggctggtcct ttcttttatg gcgatgggtg tttgaaagtt 61321 gcacatacca aaataaactc actatttgtt gacccaaagt aaaaccctga gggtatgaaa 61381 gggaagggct catgcttaca tggctgagat aaaagctgtc tcaggaagct gggtgtggtg 61441 gctcacccct gtaatcccag cactttggga ggccaaggtt ggaggatccc ctgaggccag 61501 gaattgaaga ctagcctgag tgatatagca agaccccatc tatattttta aaaagggttg 61561 gctgtctcgg gacttttcga aatagccctg caagacattc ctgttttgga atcacaacaa 61621 tttagataag atgcttttga aagaacacct gcctaggcac agcgtctcca ccaatgaact 61681 gatgccaact gtggttttga gtcttcataa ccggtgaact gtttgcaagc agcttattta 61741 aatctctttt tttgccaata caaccttccc tttgccctcc cctcttgaga tRcatatRtg 61801 gcttgccata gctgagcatc ttaggttata atgctttttc tcttttttaa tttttttttg 61861 taaatttata tttattttta tatttattta ttttttgaga cagggtcttg ctttgtcacc 61921 caggctggag tgtagtgcat aatcgtagtt caccactgcc tccaactctc gggctcaagc 61981 agtcctccca cctcagcctc ctgagtagct aagacaacag gcatgcacca ccatgcccag 62041 ctaatttatg tatttacttt attaaaaatt tttttttttt ggtagagaga gtctcactgt 62101 gtttcccagg ctgggcttga actccaggcc tcaagtgttg ctcccaactc gacttcccaa 62161 agtgctggga ttacaggcat gagccaccat gcccagcgta taattttgta ttctaattcc 62221 tgaataaatt caacaaattt ggaggtgttt tttgtctgat ggttcctttt ggtcgacaac 62281 tggaatggga gaggaacagt gtgagggcaa acatcaagtg ctttgctgag ctccgggtgc 62341 tttttttgga taagacacag aaaatacaat aagatgtctg taatgtttga tttttttcca 62401 ggtttcttga ggtataattg acaaataaaa attgtatgga ttcaaggtgt acaattcata 62461 tacattgatc cacatatacc ttgtttgatg attaatcaaa ttagttaaca catctttcac 62521 cccagcagtc aatgtgtgtg tgctctgtta gcaaatttca attaataata tagcattatt 62581 aactatagtt actatgctgt atattagagt ctcaggattt accatcttat aactaaaatt '62641 ttgtagcctt tgaccaacat ctctcattcc tcccacccac agcccctggg aactaccatt 62701 ctactctgcc tctatgagtt ccactttttg tgtttctttt ttccctttcc tttaattttt 62761 tttgtatttg ttatattacc aaaccatgtc aaaagagttc cactttttag attccacata 62821 ttattagctc gtacagtatt tgtctttctg catctggctt atttcactta acataatttc 62881 ctccaggttc attcgtattg ttgcaaatgg caagattccc ttctttttta tgactgatga 62941 tgtctgtctg caatatttga aaaaaaaatg cagatcacgt gaatgattgt ctaatgtaat 63001 tctattcatg taaaattgtg tatgaccata acagtcactc attcattgct tcgagttctt 63061 tacaatcgga tgccatattc ctggggctct gctaggcctg aaatggggag taggagttac 63121 aaggttcctg ggatatcagt aattcatatt tgtgaatgtc ttagataagt gggctaacat 63181 gtacagaaaa tggtcactaa aatgataaca ctgtttgtta taaaatactt ttctgtattc 63241 tcttgaactt ttttttttta tcatttatta gactgcttca gctgccataa caaaatatca 63301 cagactggat ggattaaaca acaaaaattt atttctcaca gttctggagg ctgggaagtc 63361 caagatcaag gtgtcagcaa ggtgggtgtc attctgaggc atcttctctt agcttatagg 63421 ctgaggccat tttgcgttgt gctcacgtga cccctttgtg cgcatgtgtg gagagagagc 63481 atgggtgctt cttcttataa ggatgctgaa agttatgaga acccgaccct tgtgacttca 63541 cttaatctta attacttcct tagaggcccc atctccatat acagccacaa tggggattag 63601 ggcttcaaca tatgaatttg tggtgggggg acgcaaacac tcagttcata aacatatgat 63661 ttttttttcc ttgatcggca atgaaaatag aggtatcttc attttα'^aa gtgagagtca 63721 acctgtgctg aattagagtg atgacaagag atactttaag gggaagcaag 63781 ggatggggac gcatttgaca gtctgttctg ctgtgctcct ggaagtgaga gaggctgagt 63841 cgggactaac ctttgcacgg atgagataga gaactgagag agaaaagtat gtatttttct 63901 atgaccacct aaaataaacc tggagttgaa gggaccagca ctctcaacct aaccattctg 63961 ggaataaaaa tcatagagac ctcgctgatt aatggaaaca atttgccttt cgattttcta 64021 agcaggtgaa tgcaaggagt ttgtgaactt tactgctgtt ttcgtgtttt tttaggtgaa 64081 gacactgaca gtaagaaaag gcaaaccaca ggtccaagac aaagtggtca agtcaagaag 64141 gaactcaggc tgtgtagtcc tggtgcaagg ctccttgccc ccagcgtggg gtcacactgg 64201 ctggctgtgc tcccacgaca cttagtgcat actgggctcc tgacttaata acacgtgttt 64261 gaaatggccc ctgattccca atctgctgca ggattctgca gatgcgcaac aggccacagt 64321 ggaaggtctt ggagagaatt cagagtggct tgcctacgtt cccatgaccg tttgtcctaa 64381 tgggagaatc accattttgg aaaattcttt tggaggccag gcgcggtggc tcatgcctgt 64441 aatttcagca ctttgggagg ctgaggcagg cagatcacct gaggtcagaa gttcgagacc 64501 agcctgggca acatagtgaa accccatctc tactaaaaat acaaaactta gcctggcatg 64561 gtggtgcaca cctgtagtcc cagctacccg ggaggctgag gcaagagaat cgcttgaacc 64621 tgggaggcag agattgcagt gagccaagat tgcaccactg cactccagtc tgggcaacag 64681 agtgagactc tgtcttaaaa aaaaattctt ttggggctag ctgtgaatga gctcagaggg 64741 aaaggcagga gccttcaggt agggagcagc atgattccac cccagattca ggcctcctgg 64801 gtgtacagag ggcaggagga tcctccccag gtatgtgaga ggaagggaag atgaggtgtt 64861 tgggtcagtg aacacactag aaatccaggt gctcactgat ggattgtgct tggcatctct 64921 gttctgccaa tccagcctca aagcaacttt ggaagaggat ttatgcgctt cacacgcttc 64981 cagcttgggc cagagcatct gcacactccc ctgggtatag gcctccaggg cttggagcaa 65041 cggaagagct aagaattgtc agtgctcagg aggcagattt tcagggagga tcctcttgca 65101 tctcaggaca tggaaagggc taacagagcc ttaagtgtcc tgtcccagtg ctggggtctc 65161 agaaggcagc tctggggcat ctccacoatg gtctgacttc tgctgcttga aaccattctc 65221 cctcacggca caggcaagag tcctcaagga agagcacctg ggcacctggg ttgactcttc 65281 ctctctccag gtggagagaa ccagtagagt ttacctgggg tcatgctctg agttaatccc 65341 cctctgcatt tctttctctt ctcaggggcc tgggctcagc cttgactcag ccttcctcag 65401 tgtctgggac ttggggtaaa cagtcaccat cttctgcact ggaagcagca gtgacattga 65461 gggttctaac catatctctt cgtacctaca gtgcccagga actggcccca cactcctcat 65521 ttattatgtc cattctcaac ccttaggggg tcccagcttg attttcagtc tctaggtctg 65581 gcaacacggc cttcctggcc gtctttggcc tctagcctga agatgggcct gacagtcact 65641 gtttgttctg ggacaaccat agcattttta cccacatgtg ctccaagtcc acggggaaca 65701 gagaccaaat ctgccatgag tgaccagtgt cacagggctt cacaccagtc aggcagtcag 65761 atggtggtgt ttgctttcat ttctgctcat catcccaagg gggtggacgc ttcaggaatg 65821 ggaccctatg caactctcat tgctctttat aatgatgtgg gaaagagaaa ccatctccca 65881 ggggacttgg ctttgcaaaa atgaaaataa aagctccctc ctccctctgt gcagtgaatg 65941 gcctctgaat gcagacttgg tctccctctt cagtggcaag gttggaggaa gaacaacagc 66001 ttcttattgc actgagacaa ctcaggggcc acctcatttg aagatgtgca tcagtgtgag 66061 cccaggtatt cctgtactca atgatcgagg tctctctcct taccttgtct ccacctggat 66121 ctgaccaaag tgactgagat gatccaggtt ctagttttct cttgctgtgt aacaaatttc 66181 tgcaaaccta gcaccttaaa ataacaccca tttatgatct cccagttttc ctggatcagg 66241 agtctgggat cagcttagct gagtcatctg ttcagggtct tcccaacctg aaatcagggc 66301 attagcttgg ctgtgttttc atctggaggc tcaactgagg aagattctgt ttccaaactt 66361 actggcagaa ctcacttcct tgctgctata tgactgaagc caaggttttg gggggctatt 66421 tgccgtcagt gcccgaagct cctagagttc ctagaggtca cccatagctc ttttgccatt 66481 taatgtaacc taatgaaaag agagatattc catcaccttt gccatattta ttggttagaa 66541 gcaaatcaca ggttcttctt gaattcaagt gaagagattg tgcaagggta tgactcattt 66601 ggggtcacca tagggtacat cctcagtaat tgtgtgcagt caggactagg ggaggagatt 66661 ctaattatac agagaagact tgtgccttct attcagaagt taagaagaca tcctttactt 66721 tttaagacac tctgtcattt tattcattga tttttagtgg tgttctagaa agcagatttt 66781 ccaagcgtaa agcacaataa tgaaatggca gagctgatga cagttcagat gctattagat 66841 gggtgtgcag atctaggttt agagccaagg gatggagtag tgtctcttgg tctccactgt 66901 ggagaggccc cttgggaaaa cccaccaccc ccatttctcc tcactccctg cctggaccca 66961 tggtctctgc ttggtctggc ctatgctatt aaaaatatac ttcaatgatg atacttatgt 67021 cttgtgtttc tctacactct ttgtctattt gcagtggaca gcacactgca tgctgatatc 67081 aaaaattatt ccagcaaatt caaaaataag acagtaaaaa gaaataaaag acagttttac 67141 aatggaagag acagaaatga atagttgcac ttatgtttga tttagctgtt aaatctaaga 67201 gaaccaactg cgaatgagta gagaaagcgg tcgttgctgc atactgacca ttactagtgt 67261 gagatactgg gggagctagg atggcctcct cttcctcttc cttctcttgc ctctcctctt 67321 ctttcttttt tggtaaatat ctttcctaga tcagcggttc tcaaaacttt cggtctcatg 67381 acctaatcac ctcccagagg ccctacctcc aaataccatc acattagggg ttaggtttca 67441 acatatgaat tttgggggga cacgaatatt tagtctagaa tgctttttca agaaaaggag 67501 gacagaaaga tcatactaca gtggctttta aaaattagat atggacagct tttgtttctg 67561 gaatatgtca gtgtgctgtt gtgcaaagca tcccgggaac aacacctcag aatgtttcct
67621 ttctgtgcct cagtaagact tgaaggctgg tagggtagcc tgagtcgtgg ctttcaatga
67681 tattcaggtc ctaattcctg ggacttgtga atactgcctg tctttgtccg ttttgcattg
67741 ctgtaaagga ataa'bcgagt ctgggttatt tataaagaaa agagttttat ttggctcagg
67801 gttctgcaga ctgtacaaga agcatggtgc ccacatctgt ttctggtaag gacctcagaa
67861 agctttcaat cttggtggaa gggggaggag gcatcacatg gcaaaaggca gaacaagagg
67921 gagaggagag gtgccacatt cttttaaaca accagctctc tcatgaatga actcactgat
67981 ctccacaggg agggcaccaa gctattcacg agggatttgc ccccatgacc ccaaaacttc
68041 ccaccttctt tttctttttc ttttttttct tttctttttt tttttggagt ctcgctctgt
68101 cacccaggct ggagtgcagt ggcgtgatct cagctcactg caagctccac cttcccggtt
68161 cacgccattc tcccacctcc ctagtagctg ggactacagg cacccgccac cacacccggc
68221 taattttgtt tttgtatttt tagtagagac ggggtttcac cgtgttagcc aggatcattc
68281 tttttctttt tcttaacctc tgggagcaca gaacataaca taggatttgg aggggacaaa
68341 tatccaagct atatcattac tttatatgag aaaaggggtt ttgcagatgt aattaaggat
68401 cttgagggag acattatact ggattatgca atgggcccta aatgtagtca cagatgtcct
68461 ttcgagaggg aggcaggggg agatttgagg acagaaaagg agacatgaac agcggtggga
68521 tgatgcagct acaagccaag caatgccagc agtatctagc agctggaaga gtcaaggaaa
68581 cagattctcc cctgggacct ccagaaggaa ccagtcctgc tgataatttt atttcagttc
68641 tgtaagcctt cttttggatt ttagattctc agaactgcaa gaaaataaat tgctgttatt
68701 ctaagcccct aagtttttga caacttgttg tagcaacaat gggaatctga tatagctaag
68761 gtaccataca ttttgacatc ttctctatat atggtaccat tctgatttgt actgtgcaag
68821 gcttccttgc cctctcactg gtaaaccttg gtatgcagga aattattttt gtacattctc
68881 cagtatatta actttggtta taggtgatac attatatcta ttgcaaatta tcattttgtc
68941 tcctaatttg catcaaccat gctgcttgtg tctatttatg actgattgtg taggaaaatt
69001 cttcaagaaa aaatcaaata ggatggtaat tcctgcatct tggaatgcct aaaaatcttc
69061 tggcaataac tgattacata gagaaaccat ctgtttactt ccttcaggaa ctacctggca
69121 ccttctagtg ctttcccttg tggaccacag gtgatggagg ctgggcccta atccagcaca
69181 cacctccagt ctagcgggct tctggtccag ttatttacct acaggtattt tattgatgtt
69241 attcttcctc tgatgacacg ctgatttcaa aacatgccta aaaccaaaga cacaggatct
69301 ttgaagtccc ttccctcaaa tatctgaggc ttttccccaa ttgagcagca gttcacatgc
69361 ttgtaagttt taaatgtgtt aRgcatttta ctggaaatgc tttatatgct tcacactcca
69421 caggacttct tattcccagc tcattgattg aggaaagtga aatctgccat ttgctgatgg
69481 gccaatgtca aggccaatgt cccagatccc ctgtcaggac aaaagtgtga tgcagccaga
69541 agtcagccct cacctgcaaa tgctctcctg aacttgccta ccctggaggg atctgctcac
69601 caggcagatt ccaggcgatg cccaaagcac tccctaataa aactctgtgt gctcatctcc
69661 gccttaactt ctgtttccag gagacccagc ttgtgaccat tgtttccagg aagattacca
69721 attgtctctg tttgccctgg accgaaggtt ttcccaggac atagatcttt cagtgctaaa
69781 acaggacagt cctgagttat cttaggacca ggaatgtcta tgaaaggaga cacttgagag
69841 atgggattct ggagccatgg agaccccaat atgggggagt tggaggtagg ggcgcgaggg
69901 tagatgcgca tatagcccct accatggtgg agcaatgcag tgtgtaaact ttttaccagt
69961 ggtgaatagg gatgatttac ctataggagg aaatgaaaaa gcaggaccat gagtcagaca
70021 tttgagagac gtgaagaaaa cagaacatta aaggaaccaa ttccacagct ttctctgttg
70081 gaacagtgtg gtagYactga tactaagtgt gattgatgat ttgcaacaga taaaatgctg
70141 gaatgattaa ttagcaatga aaaactagaa gatgtggggc aacaaggctc cagctccatc
70201 ctcttagggt accagctggg ctcaagaatt gacataaaac agattcgcag gagacaaaac
70261 atacaaattt acttaataca agtttcacat ggcacaggag ccctcacaag gaaatgaaga
70321 cccaaagaag cagttagagt cagttactta tataaggaat tggtcaaaga atagtcagtt
70381 gtgaagaaac gactaaaata tgtggggaaa aaagccccag aatctggtgt atccaaatag
70441 gactccactc tgatgacagg tttcttgggc tgagattcct ttgagcccca tccaacttct
70501 tcccagctgc tgagaagaga aaatctttta gaggcttcct ggaattttcc cagtccacac
70561 tgattttgct taaaagataa aagttattat aataaggtct gttcagcagt atctcagttt
70621 tgacttctca tccctgagga taagaatgtt gcttttcctt ctagcatagg gagggacagg
70681 gtttttcaca tgggactttt gtcttctgct tttaagaaac agaaaacagc agaatgatct
70741 ttttgcaact ggtgttttca agtgcctttt acttaaatag tcaatttgct agaccggtac
70801 ttttttattt cttttctttt ttttttatta tactttaagt tttagggtac atgtgcacaa
70861 catgcaggtt agttacatat gtatacatgt gccatgttgg tgtgctgcac ccatcaactc
70921 gtcatttaac accaggtata tctcctaatg ctatccctcc cccctccccc cacaacaggc
70981 cccagtgtgt gatgttcccc cactgtgtcc atgtgttctc attgttcaat tcccacctat
71041 gagtgagaac atgcggtgtt tggtttttcg tccttgtgac agtttgctga gaatgatggt
71101 ttccagcttc atccatgtcc ctacaaagga catgaactca tcaattttta tggctgcata
71161 gtgttccatg gtgtatatgt gccacatttt gttaatccat agactggtac atttttaact
71221 ccatcagagc gcaagccaaa aagcttcctc ctgggctgaa aaaaaaagct tccccctggg
71281 ctgaaaaaaa gcttcatctt gggctggaaa agctttttgg cttgcactct tcctctgggc
71341 ctgagcottc cctgccctct catctgcttg gacagagggc agggaagact gaagcccaga
71401 acttaatcct aagtgtagca gaccatcggg accctgacag ggtaacagtg gggtactgag
71461 tcacgggacg aggccatctg gattcacgca cctgaaaatc ttgatgtcct tgaattttcc 71521 aaacctgcag aagtggccct cccttactca ttgaaaccca tgtctccctt ccttttgcct 71581 gaagatgatg cagagacctc tgacctgtga gattgtatcc accaccacaa gtgtccccag 71641 aaatctgcca ccacctccta ttaagaccaa Wggaccaata actagaattg agtcccaaag 71701 aaacctggca aggaatgtga tagacctgct gcagaactaa ggagctgcag aactaaggag 71761 ctScagaacc agccaacacg tgcagtgggc cccaggcgag tgtgtatggg gctggattct 71821 gagggtgtaa gatcaaagtg caaaacctaa gattggattg gggagggttt atctgctgga 71881 agcactctcc tgggacgcag gctttaaccc cctagcaaag acattgaggc attctgcaag 71941 cccgccgcaa ggattgccct taggaggaca gagaaagcaa tggtccgtac caagcaaggt 72001 cataatgcca gatgtgctgt ggcagatggt gggagaggaa tcaaaaggtc ctgggaggcc 72061 aggtgtggtg gcttatgcct gtaatcccgg caccttggga ggccactgtg ggagaatggc 72121 ttgaggctag gagatcaaga ccagcctggg caatatagtg agaccctttc tgtacaaaag 72181 attaaaaaaa attagtcagg catggtggca cactcctgta gtcccagcta ctagggagtc 72241 tgaggtagga ggattgcttc agcctgggga gtttaagact gtagccagct gttccagcct 72301 gggcgacaga gtgagaccct gaaaaaaaga aagaaagaga gagaaagaga gagagagaga 72361 aagaaagaga gagatggaga gaaagaaagg aaggaaggaa ggggaaggga agggaggaag 72421 ggaaggaagg agagaaagaa aaaagaaaag aaagaaagag agagggagaa agagagaaag 72481 gaaggaaggg agagagagag ggaaagaagg aaagaaaaga aaagaaagaa agagagagag 72541 agagagaagg gttctgagag tgcttagctg gaaaggcaat accaggtact catggacatc 72601 tccccaagtg agagaattct caggagggcc tggggactgc agtctgtaat tgattgataa 72661 gcggtgcact ggtgagagaa atccagcatc cttgagaaac tcagtgggta gttctctaga 72721 ccaggccagc aggggaagct ggtaaggaag ttgatagcaa tacggatgga aggaccatgc 72781 agtgatagtg tcacattgtc agggcttaag cataggagcc aggtgtgtgg caaaagatga 72841 agtggcagca aggcacctga cattgaatga tggagtcagt cagtatagca tggcacctct 72901 cggggcaaga tgcataagcc accaagaaag ggacagcttc taatcttttt agcttatttt 72961 ttattttttt gttgagatgg gggggtctca ctaggttatc cagagtagtc tcgaactcct 73021 ggcctcaagt gatcctcctg cctcagcctc ccaaattgct gggattacag gtatgagcca 73081 tagcttccgg cctagctttt aatctatatt ttcagaggga agtaaggaag aagggtcaaa 73141 ccccattaac aagtttctga aagagcctaa cttgcaatgt ctagtcattc cagagtagga 73201 tatttacccc aaggattaat tcactgtagt tgctgtgata aatgaccaaa ccataaactg 73261 agtgactaaa aacaaagacc tctaaatgat tacacctggt atctttggag gagccattca 73321 atctactata cgctgcttcc aagtccttgt agaaaaacat acggaaatca tgcatcatcc 73381 catgggtgga agacctcgct ccaggttagt gaactgctct gatttgtgac tccaaagatg 73441 gacattggtg acagcaaaag gaggacccag aatctcgtgt attcaaacag aactccactc 73501 tcatgagagg tccttggatc tagacccttg ggtcaagctt cctctgagca ttgtccaaca 73561 tcttcccact tgctgagaag gaagattctt tatgtgggcc ctggaatagc ctcagtccag 73621 cctgatctct ctctcttccc tcctgaagtt ttatttcaaa cattttgaga ctctaaaagg 73681 gtttctttgt ctcatttctc tcctccagtc ctactaccag ggttgtcttc aatttatatc 73741 caatgtttct ctggcctgga actgagacct acctcccaag cagcgacccg acccgtggag 73801 tggggtccct ggggcaagcg aatggYcctt tgcctggaga acctgggcca gcagtcatgg 73861 caggtgacac attccattct ggggcgagga accagggagg gagcactgca tagatcagaa 73921 gggtaaacaa ggggagggag aaggagcaaa cttagatttg ggagggaaag agggaagggt 73981 gcagaggaag cagaacacgg atgactgggc ctccttcagt tgttcttgtg actagaccct 74041 cccatctaag ccaccagggg acaggaaggg tatgagtttg aggactctaa tggccccaag 74101 gtactactgY ggtttatcct acttgtaggc ggtcccaaga gctttgttct ctccagggac 74161 tcaaagggga tgaacttgca agtcttttgt ctccactcag tgccttctct gattaggcca 74221 ataaacatcc ccaRgcctca gtctcaRgcc actgtagggg ctgaggacag tgagaaagat 74281 gtgtgagcat ccaccctttg gagaggttga gtaggaatga gaggtcccct gccttgcctt 74341 tggtccttgt gaaggaagat gttggatatt tcccagcttg gctggaatcc gttcctcacc 74401 ctccccattc agagcaggga tgagtaagtc ctccgctttc tccgcaacca caattgtgtt 74461 tttcaggaaa ggataaagtg ggaaagatca gatcccatgc ctcttcctcc tctaagtcat 74521 gggatgactg cagagagtga gcaagagggt aaaaacagtg cactgagtgt gatgtttgct 74581 aaaggaacag ataattgcat caaaacctat ttctagcaga gttgtggtgc ttgcctataa 74641 tcccagctac taaggaggct gaggctggag gattgtgagg ccgggagctc aagaccagca 74701 tgggtagcat agtgagagct catctctaaa aaacaaaaca aaaaaccaca cttatttcta 74761 attgtctaat tggtatccac tatcagacct ttctgggaag ttttttttct aacatgccta 74821 aatgtggctt ggagactgtt tgggtgaggc caatagctta gccttcctac ttaagttgtc 74881 tccttcattc attcaacaag caggtattat tgagtggatt gttgggtgca tttgtgtgtc 74941 ctcagtagga tgcaggcatg caaYacatga agatatgata a-ttccagtca gaggaatggc 75001 ctgtgcaaag gccctgaggt agagagaaMc ttgataattg aaagaacaga aagaaatcca 75061 gtgggactgg ggaaagagag gaaggagatg agaggggaSt tacaggcagc caacagatca 75121 tgtgcagaca gatcacttga aagcagaggc aaatggctgg atgtttttgt taatgaaaag 75181 agaatttatt tgaagatctt ccatcatttt tacatgaagt gattgatgtg ttaaatgaat 75241 gaaatttaac attcatggtg tatgaaattt atggaagaaa aaYtgaagca ggcagagcat 75301 tttatggcct ttgggcacaa gagagtgaag cttgtcccat gtagagtcat ggagagtgac 75361 tgggagattc accattagag ttggcagatt ggctcttgga ttgaatatgg ggcatgaagc 75421 aaagagttgt tgactcctgg aagtttgact ttgtcaatgg cgtggctact actttctttt 75481 attaagagag agacagatgg gagagcaaca ggtttgaggg aaacacataa agtgctttgt
75541 ttggactcat ggggattttt tggagaagac agacaagata caagtaacaa gatSatgatg
75601 tctgtaatat ttgggtacaa aatgcaattc ataaacctga ttgtataata tgatcctatt
75661 tatgtaaaat catatatatt tagttacctt gattcaattt atacatcact aattgtttcc
75721 agtaatatta accaaaggtc tactatatgc caaataacct tttatggcta gagatagaaa
75781 aatgaacaaa acttactatg ttcatcctta tggaagatac ataattcata tttgtaaaca
75841 tcacatttaa gtatggcaag aaatctggtg ggatggttcc taaaatgaaa caatagtttc
75901 ataggtttgt gagaatttag gtgatatttc tacttaactt tcatgtacac taaaaatttt
75961 tcctataact caaaaaaatt ttttggaggc agattagcaa gatatttatt ttggaaaagc
76021 aagagtcaat ctgaattgaa ctggagggat gctgagagtt ctagaacttc atagagagaa
76081 agaaaactga gttattgggt gggggttaca cctatctttt tgtcttttct tttctttctt
76141 tctttttttt gagacagggt ctccctctct cactcaggct gaagtgcagt ggtatgatct
76201 tggctcactg caacctctgc ctccctagta gctgggacca ccacaggcat gcaccaccat
76261 gtccggctaa tttttaaaat ttttttggta gagacagagt tttgccatgt tgcccaggct
76321 ggtctcagac tcctggactc aagagatccg cccaccttgg cctcccaaag tgctgggatt
76381 acaggcgtga accactgcgt tgggccgctt atcactttaa tgtctaatga gaggcccctg
76441 gagctggaac ccttagcccc agcctgaccc ctgacccttc tcccatgtcc tcaaattcaa
76501 aaactcacct ctggctgtcc attcagttgt tctcaagtct gactgtgctc tttgtccttc
76561 atgaactaga actagtcttt ctttcaaacc attttgatRa gctgatcaat gtctcctcat
76621 accatttttc tcattcagcc ttcctcctgg ggttggtttt atttaccatc taacaatatt
76681 tctgtaaccc tgagcctcta gagctgcctc aggtgcacta acgctgtgac taggctgccc
76741 tgggacaagc taagaggttc tgcgggggag ggtctgRgcc cctggtgtgg cagaaggtgc
76801 atccatcctg gaggaagcca gaggaccaag ggccaggagc ctggggggga ttagaaggga
76861 ccaggaggcc tggtcctgag ctgggatggg aaggaacggg aaggggcagg agcaggacag
76921 ggatttccag atccactcag ccgcctccct ggggcttggg ctcctccatc tgaggccacc
76981 agggggcggc aagggcttgg atttggggaa gggaattgct gtggtttaac cagggagtgt
77041 atgaagctcc atgggctgct tcctcctgag acccttctaa gggagaagtg gggagtggaa
77101 tgtccaaaat atgttgtgtc ccaatatgtt cgtttctaga aaggctaata aatagcctca
77161 gcccccagcc tcagagcact gcatagaaag aggacagagg tatttgaggg tcattctgag
77221 agaacctaga aggtattgag tgggaataaa cagtccccag ctctgccttt ggtccctggg
77281 aaacagagtg cctggatcac tccacagtgg tcatctggga tctggtccga ggtctcttcc
77341 ttgattctgt tccctgagcc ttttttattc tattttggat cagatccatg cacacgtagc
77401 tcagagttgt gcccaagatt tcataaggaa tttacagggt tttttccccc tcaaacttat
77461 ttctctccac agtctgccca ttatttatgg ttccccagac tttccctttt ggtcttctgg
77521 tgaaaaaact gtgactttaa taagcctgcg gtgctgtgca cttcctgcaa ctgagtccgt
77581 gtccacacca aacaccaaga ggacaaagag taacaaaggt aaggggatta agtgccactg
77641 tcttggaaat cagctccaaa gatgagaaaa gaaagtccgc acagtctcta ctgtccctgc
77701 tgtcaccacg aagaatttcc tggaggctgg atcatgtaga atggagagaa gaacaaacat
77761 aagaaaagga aaacgtggac atttcaaatt tgccctgagc actaggggtt tcctttctca
77821 tcttcaagcc agaacagtgg agcttcttct ggaacttttg tggctgcaca ctggtacccc
77881 ttccaggtat tggtctgccc tgagtgcagg ccacaggaca ctgggggaaa atgggaagct
77941 ctctgctggt ttgattttac taaaagtgct agtatctccc ctagtctgcc tccagtgatt
78001 tgcttttcca agtaatcaaa ttgatgctcc atggattctg tcccaggttt tacagctggg
78061 cttggagaaa atgtggtata tgccactgca tccgaaatgg aacctgaaaa cagtaataat
78121 attctaaacg tgaaaattaa atcaatctga atcacaggca gtggaaaaca tcaaaagatg
78181 aaagagtttt aaaagtgatt tgaggaaaga aacagggaga aatattttca cattggttct
78241 atgtttactc tatttatctt ttttaattta attttaaaaa tagagatgag gaggtctcac
78301 tatgttgccc agccaggctg gtctcgaact cctgggctca agcaattcac ccgcctcagc
78361 ctcccaaggt gatggattac aggcatgagc caccacaccc ggcccctgtg tttcttatgt
78421 gctccttgat taagaagagt tgttttttgt ttttgttttt gttttttgtt ttcttgagat
78481 ggagtttcac ttttgttgcc caggctggag tgcaatggtg caatctcggc tcactacaac
78541 ctctgcctcc caggttcaag cgattctcct gcctcagcct cccaagtagc tggattacag
78601 gcgtgcacca ctacaccagc taattttttg tattcagtag agacggggtt tcaccatctt
78661 ggtcaggctg gtctgaaact cttgacttca ggtgatccac ctacctcggc ctcccaaagt
78721 gctgtgatta caggggtgag ccaccatgcc cggccaaaaa gttggtttat tgtagcttgt
78781 ggatacagtg agatggaaga aaacccagaa agcagaaata acacttcttt gtaatatgag
78841 gaaaaaaata aaagataaag ttgaatgaaa tatttcatat atatatgttt catatacatg
78901 tttcttatat atgtttcata tacatgtttc atatatatgt ttcatataca tttcatatat
78961 atgtttcata tacatttcat atatatgttt catatacatt tcatatatat gtttcatata
79021 catttcatat atatgtttca tatatatatt gcaacatata tatgcaatat tcatatatat
79081 agccatatat atgtttcata tatatattgc atatatatgt ttcatatatg tttcatacat
79141 atgtttcata tatatatagt tttttacaga gtcttgctct cttgctctgt cacccaggct
79201 ggagtgcagt ggcctaatct tggctcactg caacctctgc ctcccgggtt ccagtgattc
79261 tcctgcctca gtctcccgag tagctgggat tacaggaacg caccaccatg cctggctaat
79321 ttttgtattt ttagtaaaag tggagttttg ccatgttggc cagtctggtc ttgaactcct
79381 gccctcaagc gatcctcctg cctcagtgtc ccaaactgct gggataacag gcgtgagcta 79441 tcacgctcgg tctgaaatct ttagataagt atgaagtcta tataaaaaaa aaaggctctt
79501 actaggctag aaatggccca ttttagaagc tttcattttc cacctcacac tcatctctct
79561 atagatcctc tgcWtgggca gtgggtggRc cYtgtggtcc tcagttctca ggctgagaac
79621 cagaggctga aaaggacaag tcacctgctc atgagtggga agggcaggat tgggagcagc
79681 ggactgtggc tccRttgctc cttgtttcct ccacaggccc ctcccacgtg tgcccctgaa
79741 tgacctagaa tgactaatgt gctcaaggga tttgatgctg cagatgcttc ggcatgaagg
79801 gtgggggtgg ggatgggaca aggctgcagc tgaagagatg ggagaccaac aacacacagc
79861 aggggtttcc atggggtggg cacccccatt tcctcacagt ttagagagcc tggaggtggg
79921 tgccaagcag agggcaccat ggcgtggccc aggcctggag gttcacagag acacaggcac
79981 aacacagcag agacactgag ggccaggagc tcactcagat gagggacttc agcagatctt
80041 tctctcttga ggaaattagg tacaaaggaa tcaagttcct accatcagaa tagacaacaa
80101 gttttaatct cctcagctga gccccgctgt cSagggaagc agaagtctct gagcccggcc
80161 caggtgagag tggggtgagg agaggagctc agggtgcaga tttgcatgaa ggccccaccc
80221 tcctctgagg cagaggggat aagacaagtc tgggggtagg cccagcgctg gggtctcagg
80281 aggcagcgct ctctcgggac gtctccacca tggcctgggc tctgctcctt ctcaccctcc
80341 tcactcaggg cacaggtgac gcctccaggg aaggggcctt ggggacctct gggctgatct
80401 ttggtctcct gctcctcaga ctcacctggg cccagcactg actcaccaga gtgtatttct
80461 ccctctttcc agggtcctgg gcccagtctg ttctgactca gcctcgctca gtgtccaggt
80521 ctcctggaca gtaggttact atcttctgca ctggaaccag cagtgacatt gggggttatg
80581 accttgtctc ctggtgccag tagcacccag gcaaagcccc caaactcatg atttatgatg
80641 tcgStaattg gccctcaggg gcccctggtt gcttctctgg ctccaagtct ggcaacacgg
80701 cctccctgac catctctggg ctccaggctg aggacgaggc tgattattac tgcagctcat
80761 atgcaggcag ctacaatttc cacagtggtc caagttcatg cgtaatgaga ccaaaacctg
80821 ccctggtctc tcaggctctc tctcgctatg aaggtgcttc cttaccctgt gcagaagagg
80881 gYttcatgca acatggcctt gagaatttca tccactctca gcccctctcc cctccaacaa
80941 tgaaatgcaa aagaaacatg ctctctggtt aattgtctag ggacaatggc agcttcttct
81001 tttcctgtgt gatatggtcc tgcatggtga ctctttccaa cttttcaatg gcagggacag
81061 agcaatagga tccatctgct cagtaccatt tctgcagttt tctatgaaac cctcacatta
81121 aatgcctcca tctcccacac tgtgctgact ggcctgggtc tgtgctccag aaataacgtc
81181 ttactcaact ccatatccac cacgctgtag taaggatggc tgtctttgct gggttatttg
81241 ttcctctgca ggtctcttcc caaagcaaca ccaactgaga gaagatctga agatgttgag
81301 agagtgagca taaaagtaaa gggttttatt tatttgcttt cttttttttt taactttgtt
81361 tttatttatt tttttgatac agagttttgc tcttgttgcc caggctggag tgcaatggtg
81421 caatctcagc tcactgcacc ctctgcctcc cgggttcaag cgattctcct gcctcagcct
81481 cctgagtagc tgggactaca ggcacgcgcc accatgccca gctaatgtag agatggggtt
81541 tcaccatgtt ggcctggctg gtcttgaact cctggcctca ggtgatctgc ccacctcagc
81601 ctcccgaagt gctggcatta cagacatgag ctaccatgcc tggccttgtt tttatttttt
81661 agagttcctt tgtctttgac ccgatgtcta tctatggggg cttagccagg gcacatttga
81721 gtgattgttc tttgcgagaa cattgttctt tgcagatcat taaacccctg tcctggaaac
81781 agtcctgtac ttagactgtc tccctgttag catgggctct ttcctaaatg atccacagga
81841 aagcttattt ggtggatggg tcttctgttc ctcaaggact ctatgggtag tttattttca
81901 aggtgagcca atactgtttc tagctggaat ctcatttaat ctcataacca tgagaattaa
81961 ttttttaaac ctgcagggtg ttctctagcc attttttWat tttctttgat tttgtgagcc
82021 acttttaaat tataatcatc attaaacctt aaaacatata aacttcaatt aagcagatta
82081 tatattttat tttatttttt gagacagagt ctcggtctgt tgcccaggct ggagtgcagt
82141 ggcacaatct tggctcgctg caaactctga ctcccgggtt caagtgattt tcgtgcctca
82201 gcctcccgag tagctggaat tacaggcatg tgccatcacg cctagctaat ttttgcattt
82261 ttagtagaga cggggtttca ccgtgttggc caggctggtc tcgaacttct gacctcgtga
82321 tccacccgcc tcggtctccc gaagtgctgg gattacaggc atgagccatc aagaccagcc
82381 acatattttt aaaaagtaca ttggttatta tccacatacc tttggactgc tataacacaa
82441 taccttaaac tggtggctta taaaaacaga aatgtatttc ttacactcgt ggaggctggg
82501 aagtcttaag atcaatgtgc tggcagattt ggtgtctaat gactgtctgc ttcctcatag
82561 aggccatttt ctcactgtgt ccttggaact gaggacaaag cagctcctgg taaagttcag
82621 atgtctgttt cttgcaggaa aatttctctt tacagtgatc tgggctactg gctccaacac
82681 acgcacccag atggatttct gagccggtca ttgaccatta ccattttaat attatctcaa
82741 attataaaca ttatctcaac tattgtttaa tataatatag cacataaatg tttaaagcat
82801 acatagcgaa acaagggtct tacattcaaa actgtcattt ccccaatcac ccatctcctt
82861 gtccaccaag ccccagtgcc accacttagc tcattgctcc tttggcttca ctttctggtg
82921 ctcttgcaag tgtctgtcat gtgttacaaa caacaacaaa tagtcctcca agaacgttta
82981 cctccataga atccctatcc tgtttcctga gtggacattt ccatttttaa tctggcaaag
83041 atcagtcagc cctgattagt gttttaaact catatccatc tctttctaga aaaacacact
83101 aggttaagca tctctctgtg gatgggggac cctcatgaag gcacacagct ctgagctgag
83161 gtggcctcaa tgtgtacaat ggagcaagac aaagaaaagt cccatgttct gtgtgataga
83221 cagacaggca ctgatgagag gtctccagag ctggcgccct tagtctaatc ttgactctag
83281 tcctcgtctt tccgaccctt aaacactaga attcctggtc tgcattccat taggtcattc
83341 ttaaatctag tgagctgtat ctccttcatg caatgttttt ctttcactat tattttcagc 83401 tttattgaca tataattgac aaataaaaat tctatatatt tatggtatac aacttgatat 83461 attaatacat ttgttgtaca ttgatatatt tgttgtgaaa ttactgcaat aattgtgtga 83521 aatcacaatc aatctaattc acataaaata accttacata attatcatct tctttctttt 83581 tttttttttt ttggtgagaa cacttacaat ctaccaaatt agcaaattta aagtaaataa 83641 tgcaatattg ttaaccatag tcacattgcg gtacattagc tatctagaac ttattcatct 83701 tgtatcactg tactctttta taccctttga taaacatctc cccatttccc ttttccctcc 83761 ccagtcctga cacccaccat tttactctct ggttctatga gtttgaatat tttagattct 83821 acatgcaaat aagatcatgc agtatttgcc tttctgtttc tggcttatat cagttaacat 83881 aatgtcctcc agctttatcc atgttgttgc aaatgatggg atttccttta ttttttaaaa 83941 cttggcaata tttgtgtgtg tgtatgtgtg tgtatgtatg tatgtatgtg tgtgcatgca 84001 ccacattttc tttatccatt catttgtcat cagaaattta ggttgtttcc atgtgttggc 84061 tattgtgaat aatgttgcaa aagacatggg agaacagata tctattcaag attctgattt 84121 cattttgttt tgagatatgc ccagaattgg tattgctgga ttatatggta gttctatttt 84181 taatttttta aggaatcccc atacattttc tacaatggct gtttagttta cattcctttc 84241 aacagtgtac aagagtttct tttgtccaca tccttgccaa catttgttat cgtttatctt 84301 tttgataaat tacaggcacg tgccaacaca cctggctaat tttttatatt tttggtagag 84361 acggggtttc accatgttgg ccaggctggt ttagaactcc tgacctcaag tgatccactg 84421 cctcggcctc tcaaagtgcc gggattacag gtgtgagcca ccatgcctgg cctatttttt 84481 ctatttctat aaagaatgac atttgtgtat tgataggtct agcattgaat ctggagatca 84541 ctttaggtag tatgaacatt taagaatatt agttcttcca atttataaac acgagatgtc 84601 ttgatattta tctgtatctt ctttaacatt cttcatcact gttttataat ttccagtgta 84661 aaactctttc acctccttcg ttaagtttat tcctaagtac taattccttt tgtaaatgtg 84721 attgctttct taattttatt ttcatatatt tactgtctgt gtgtagaaac gtcatcaatt 84781 tttgtatgtt gattttttta tccttcaaat ttacttaatt tagttttaac agactttttg 84841 ttattgtgga gtctttaggt ttttctacac ttatgtttat gtcatctgca cacaaagatt 84901 aggattttaa tccttctttt ccaatttgga tgcgtttcat ttctttttct tgtcgaattt 84961 ctctggttag ggattccagt gcaatgttga attaaaataa gtggagaggg ggcatcttgc 85021 cttgtgtggg atcttagggg aaaagcttct gtttttttcc cattgattat gatgttagtt 85081 gtgggctttt cacatatggt ttttattgtg ttgtggtaag tttcttctgt acctatttca 85141 ttttattttt gtttttttaa atatttattt tataatagag attaattttg ctatgttgcc 85201 caggctggtc ttgaactctt gggctcaagc gatcctcctg ccttagcctc tcaaaatact 85261 gggactacag gcatgagcca ctgtgcccgg cctatcacta ttttgttgag agttcttttc 85321 ttttcttttc cttttttttc cattgctttc ttttcttttc ctttcctttt ttcctttcct 85381 tccttttctt ttcctttctt ttctcctcct cctcctcttc ctcttctttt ttctttcctc 85441 ctcctcctcc ttcttcatct tcatttaaaa tcatgaatgg atgttgaatt tggccacatg 85501 ccttttgtac atctgtggac ataatcatat gtgtgtgtgt gtgtgtgtgt gaatctttca 85561 gcctgtggat gcgctatacc atccttgcac atgcggaatc atccttttat ccagggataa 85621 accccacctg ttcctgatgt atgatcttct taacatactg ttgaatttgg tttgctagta 85681 ttttactgag aatttttgca tttaatatct gttaatgttt atcagaggta gactggcttg 85741 cagttttttt tcttttctgg tgttgtcttt gctttgtttt ggtatcaggg tgatcccgac 85801 ctcataaaac aattttggaa gtgttccctt ttctattttt tggaagactt taagaagaat 85861 taatattaac tcatctttga atgtttggta gaacattcag ctatgaagcc atctggtcct 85921 gggcttttct ttgttgagat atttttgatt attgagtcaa tctccttctt tgttattggt 85981 atgttcaggc tttgtatttt ttattgattt gctttttttt ttaagttgac tgtttctagg 86041 aatttattta tgtcttttaa tttatctagc ttggtgtata atttttcaaa atagtccctt 86101 atgatcctat ttatttattt gagacagggt ctttctctgt cacccaggct agagtggaga 86161 ggcttgacca cagctcactg cagccacaac ctcccaggct caagtaatcc tcccacctca 86221 gctttctgag tagctgggac tacaggcatg caccaccatg cctggataat ttctttttct 86281 ttctttcttt cctttctttc ctttctttcc tttctttcct ttctttcctt tctttccttt 86341 ctttcctttc tttcctttct ttcctttcct ttctttcttt ctttctttct ttctttcttt 86401 ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt 86461 ctttctttct ttctttcttt tcttcttttt tttgtataga tgtgacctca ctgtgttgcc 86521 caggctggtc ttgaattcct gggctccaga gatccacctg cctcggtctc ccaagtgttg 86581 ggattacagg tgtgagcctt tttatttctg agaaatctgt tgtaatagct ccttttttca 86641 ttttggattt tatttgagtc ttctctcttt tttcacagtg tagctaagga tttgtcaact 86701 tYgtttaatt tttcagaaaa ccatctctta gttttgtttt tttctattat ctgtttgatt 86761 tatttctgct gtaatcttta ttatttcatt tttttctgct aagtttgggc ttagtttgtt 86821 cctctttttc tagttcgttg agttataatt ttaggttKct tatttgagat ctttcttctt 86881 ttttaatgtt tgcttatctc cataaacttc ccactYagca ctgcttttac tgcatcctat 86941 aggtttggtg tgctgtgttt taattttcat ttgaagatat ttttaaaatt ccctttggtt 87001 ttcctctttg acccaatgct tgttcaacat gtgatgtttg atttctgcct atttgtgaWt 87061 ttttccattt ttttttactg ttattaattt ccagattcat ttcttgttgt aaaaaaagat 87121 ttttggaatg atttaattct ccttacattt cttaagactt gttttgtgac caaacatgag 87181 ctctctcctg gagaatatac cctgtgcatg taagaagaca ttttgagaac ccaggtggtc 87241 ttttctcata ccatttttct cacccagtgc tcatactgag gttagttcat tttacatcta 87301 acattttctc agcctggagc taagacacct catgtacctt tgacaaagcc attctagcac 87361 aagcaaaacg gtcctgagaa cgagtatctt agccacctgg tatggcagag gacacattca 87421 tcatggaaaa agtctgagga aaagggtcaa gattagcatg gatcgagggY ctggtcctga 87481 gctgggagga gaaaaaggca ggttggagtg ggaccaaacc aggtatttct atctctcact 87541 tagaattgtg tcttggtact tgagatgaac catctgaggc caccaggggg cagcagggga 87601 ctaggttgga gaagatgatt gataaagttt atcctgacct ttaagaagcc cctgagtcgg 87661 ccgggcgcgg tggctcacac ctgtaatccc agcactttgg gagactgagg agggtggatc 87721 ataaggtcag aagatcgaga ccatcctggc caacgtggtg aaaccccgtc tctactaaaa 87781 atacaaaaat tagctgggcg tggtggctta tgcctgtaat ctcagctagt cgcgaacctg 87841 aggcaggaga actgcttgaa ccagggagtt ggaggttgca gtgagccgag atggcgccac 87901 cgtgttccag cctggtgaca gcgcgagact ccatctcaaa aaaaaaaaag tccctgaatc 87961 tcaaaaaaaa gaatgtgact ttatgattaa ctatgaacag ctgctcatta ataaaaagcc 88021 attaaaaaaa aaaaagaaat cccaagagct acttaccctt gaatatctct aaaggtactg 88081 ggaagcagtg ctccaaatct gttgtggccc agtgaattcc tttttagaag caccattaat 88141 ggtcctagtt caacatgtca ggccccagct ttaaaccact gcaggggcag gggatgtagc 88201 agtgcttaga acccacctta gctgagagga ccaagatggt tgggcagggg atgagggaaa 88261 tgagaagttc cagcactgtc tgaagtctct gggaggagga gtgattgaac tttcccaccc 88321 ggcctgggag cagcctgtcc tcacattcat ccctcacagc acaaaggaga agtcctggtt 88381 tcctcagcct ggagctcctg caatagtgag ggttaggata tctctggact ggcctgatgt 88441 cctgggcact ctcatgggtt cagtctcagg aaatccatgc ctaggctgga ttaataccct 88501 cagctgagcc tcacagggaa gcagaagtct ctgagcccag gcccaggtga gggtggggtg 88561 aggagaggag ctcaggacac ,agatttgcat ggaggccctg ccctcctctg aggYgggggg 88621 ataagacagg gctagaggca ggcccggtgc tggggtctca aggcagcgct ctcgggacat 88681 ctccaccatg gcctgggctc tgctcctcct caccctcctc actcagggca caggtgacac 88741 ctccagggaa atggccttgg ggacctctga gctaatgctt ggtcttctgc tcctgctcct 88801 cagggtcact ggacccagta ctgacccagt agagtgtgtt tctccctctt tccagggtcc 88861 tgggcccaat ctgccctgac tcagcctcct tttgtgtccg gggctcctgg acagtcggtc 88921 accatctcct gcactggaac cagcagtgac gttggggatt atgatcatgt cttctggtac 88981 caaaagcgtc tcagcactac ctccagactc ctgatttaca atgtcaatac tcggccttca 89041 gggatctctg acctcttctc aggctccaag tctggcaaca tggcttccct gaccatctct 89101 gggctcaagt ccgaggttga ggctaattat cactgcagct tatattcaag tagttacact 89161 ttccatagtg gtccaagttc ctagggaact gaRaccaaaa cctgccctgg gctctcaggc 89221 tctgtctttg ctctgaagat gcttcctcac cctgtgccag gggcttcctg caacagggcc 89281 ttgagaattc acttctctgt cagctcctct ccttttccat caattcccaa aggaaacctc 89341 ctctcttgtt tactctccgg gatatgacag cttcttcttc actcgtatga cggatgtcct 89401 ctctgaattg gaaactactt ctagctcttt aacaggaagt acataccggc agggaaatgc 89461 ctgcctagtg cagtccccat acttctYcRg atgatcctca ctttattatt tatttttatt 89521 tttgtatgca ctaaagtcgt caatgtaaat ccttacgtcg aatgcctcca cttcccacat 89581 tgtgtagcac agcctggatt cagttcttca gaaatgacgt cctcttcaat gataaaggac 89641 cactgctctg tgaacacccc actctagtga agatagccca ccttgctggg ttattcatgt 89701 acatcttttg tggaagcagt aaccactgtg taaagctcca aagatgtttt aaagcaatga 89761 aaacaaagag gaagaagtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 89821 agggtccatc ctgtttgccc taatatctca ggttgccgag ctagggagta tttgagtgac 89881 agacttggtg ccctctcagg atcctcctcc tctcacatca ctgagtccct gtcctggaaa 89941 caccctacag gtggacaatc tccccatagt atggaagttt ccaaaatggc tccacaggga 90001 agagttgagc tgaacacacc cactctttct catgggcatc aaatatatct aatttttctg 90061 gggaaatcat caatttctag ctggaatcct gattaatcga cagactgtga gaattaaaaa 90121 ttttaaaaat attcagacat atccagagtc cagtaYttaa attctctgga attttgtgag 90181 ccaatcttta aacatagtta ttattgtagt ttagagttca tgaacctgaa ttaaatagat 90241 tctaKtaaac ataaagtgcc ttagttgtct attgttgcat aaaaattact cctaaaatta 90301 acatattctt aatgagaaca catatgcatt aatctgtttt cgcactgcta taaagaaata 90361 ccttatttat aaaagaaaga ggtttaactg gctcacagtt ccacatggct ggggaggcct 90421 caggaaactt acaatcatgg tagaaggcaa aggagaagca agaactttgt tcacaaggtg 90481 gcaggtgaga agagctgggg agggtgaact tccaaatact tttttttttg agatggagtc 90541 tcactctgtt gcccaggcta gagtgcagtg gcgtgctctc ggctcactgc agcttctgcc 90601 tcccaggttc aagtgatcct cctacctcag tctccgagta gctgttatta caggcatgcg 90661 tcaccaagac cgactaattt ttatattttt agtagagatg gggttttacc atgttgacca 90721 ggctggtctc gaactcctga cctcaagtga tttgcacacc taggcctccc aaggtgctgg 90781 gattacaggc gcaagccatt gtgcctggcc ccagacactt ttaaaaccat cagctctcat 90841 ggtaactccc tacccatcac gagaacagca aggggaaaac tgcccccatg atccaatccc 90901 ttccgtccct caacatatgg ggattacaag tccctccttc aacacatggg gattacaatt 90961 tgagaagaga tttgggtgga gacacagagc caaaccatat taatgtattt gtgatcttgt 91021 agttttcttg catcaagaat ctgagagctg cttagtttgg tggttctgac tctgggctcc 91081 tcatgaaatt gcagccaagc tgtcaggcgg ggctgcattc agaggccaga gcaggtggcc 91141 aggcccagcc tgagggggct tctactgtcc ctaacctctt tggcagatgt agatgaagtc 91201 agggaaaagg ggagctcccc acagtgggta gcatcccagt gtcagaggga aggcaccatg 91261 gagcagtggg accagcacag gaccctgggg gtgcactcag acctacctgg agggatggca 91321 agaaggggca ttcacagcag ggacaggata aagggcctct gccccatgtc cagagctgtc 91381 ttggcagctc agtcacattg gttatcaggt aaatcggagg taaaccctat cctgagtttt 91441 accactgaaa atgggtacaa tttgaactag gagtgttttc agactttttc tttccatatt 91501 gacttcttaa taaccgcgat ttaatttttt ttaaccccag cctagtttag ctacatagct 91561 tccaaatgtc atgctactgt gaaactgagc aattagttac ttttatcaga gcattttaaa 91621 tttgaacagg ttccttggtc tttctatgtg taattagtca aagcttaggt atggagtaga 91681 ggatctttac caaatgcttt tctagactct gttttcattg gacttatagc agctgtttca 91741 cctgggccat gcaggaatag gagagttgtt gctccttgac atagcatctg taacatgcct 91801 atcctttctg ccactataaa aatagctgtg ctttctctcc actcgtctct cctgctgttt 91861 ccctgttttt cctattaaat atgcagtgtt tgcaatccat cttgcattct gagaagatga 91921 ggattttttt tcataaagaa tgacctaaaa tagctctaaa gatcatcaga agtcacaact 91981 acatacttac tggagtgcaa taccaaatgt ggcctctgat tacagtaacc tggagcttgc 92041 agtttaagca attcagtccc tgtcacttgt ggacagattc gtatgtctgt ggatgagaag 92101 atgagcaacg gtcactcctg tgctcatgca cagatccctc cctgcccctg tcctcaaatt 92161 cagactgaag tgcatggccc tggagcatta catgagtgat ggtttaagag cacgttggtg 92221 atacacacaa tattaatggt atcttaattt gcttgttaag cataatttga aaacatctgg 92281 acaaatttgg acacaatttg aagggcatat ttgataatag cttgtgacaa atgattccgt 92341 tctgggagag atgctatttt tggttctcta aatcgccatg aaatgaaaat ctcagattat 92401 aaagaaatag actcaaatcc caagtgtctt ttgcttttca ttccccatca actgtgttgc 92461 ccaagcacca tgtggacact gcttacaggg taggcagcca ccagctgaag gcaggttctt 92521 tagttgtctg agctgtcctg gagctgggaa cagcagcgtg tgttctgtgc ctgttggtcc 92581 ctatgtagca cacacccact gcatattctc gctgactcac caggagggct tgcagcagcc 92641 ctggcctatt ttcttctgaa gcacctgcca atggtgcatt ctcccctggt cataatgcac 92701 caggagataa acatggcatt tgacacacag gtgctaactg gatctaggac ttctaagctt 92761 aagtagactc agagtgggca atctaaaaaa aaaaaaaaaa ataggttctc aatcggtagc 92821 ccaggctgga gtgcagtggc tccatctcct acctcagcct cctgagttgc tgggacaaca 92881 ggtgtgtgcc accatgccca gccaacagag agggcaatat ggacttgaaa tggacaattt 92941 ccattacttg aaagtttttg agaaagaact gaggtttgtt taagaggaaa tcagccagag 93001 actcaaagtt aaatctatca ggcccaaagt ctctttgggg aaaggagact cttggacgtg 93061 cacgcagcag caagtggtat gggatccctt ctctgtgggg accttggccc aactgccaat 93121 cttttcaact gacccatttg cgttttccat aagtgctcgc cagccagcag ctgtgactgc 93181 ccctcagtct caggaaggga gaaagaagga aacaggagca aagcctcttt caatcttagc 93241 aaataaagtg ggagctttga cttttttgaa cacccagatg acaggaaaga ggaggaaaat 93301 tttcctaaag ctgttatttt ttaatgcctc aggaagaatc acacccggag tctcatccac 93361 actttatttg gaggtatttg ggtctcagag tttgtgctga gttagttaat gatttgggct 93421 actgggatgg ggtgaatgtg ttttacaagt gagaaagaaa taagctttgg agtccacagg 93481 gtggaggctt attggctaaa ttgtgtccct caagattcat gcaatgaagc cttaatcaca 93541 gtgtgtgact aaatttggag acagggtctt tatggaggtg attaagtgaa aatgaggtca 93601 tgagggtggg ttcttatcca gtctgactgg tgttctcctt agaagagaaa atttgcacac 93661 acagaaatca gacaccagag atgagcaggc ggaggaaaga ccatgtgagg tcacagcaag 93721 gaggtaacga cctgcaagcG aaggagagag gcctcaggga aaatcagtcc taccaacact 93781 ttcaccctgg actttccagc ttcagcactg agaaaataag ttttgctgtt gaggccacta 93841 gttctttggt atcttgtgat ggcagcactg gcaaatgaaa acaagaggga ccccaaagac 93901 Cttgggtagg gggaacagat ggagcagggt gcaggaggca gggcagggag gggctggaag 93961 gtcatgctct gaggttcgtt tcctggatgg aactgggact ccactcacta ctgtctgggg 94021 aacttgggga aaaaacttca cctcccaata ttgattccct aataaaatct gggccctgag 94081 catagggctc ctcatcttcc tactccatat gatgcaagtt ttcttgaagt tatgcttgta 94141 aaacactcac caatgcatct ggcatatatt aagggctcaa aagtaggcca ggcatggtgg 94201 cttatgcctg taatcccaat actttgggag gccgaggtgg gggatcactg gagtccagga 94261 gttccagacc agccaggcca acatggtgaa actccatctc tactaaaaat acaaaaaaaa 94321 aaaaaaatag ctaggcatgg tggtgccctt ctgtaatctc agctattcag gaggctgagg 94381 caggggaata gcttgagcct gagaggcgga ggttgcggtg agcccagatg gtgccattgc 94441 actccagcct agatgataga gtgagactcc atcacaaaaa ggaaaaaaaa aaggttgtca 94501 ttctgcagcc cagatcgtgc cattgcactc cagcctggac gatagagtga gactccacca 94561 cacacacaca caaacgcaca cacacacaaa aactaacccc cgccccgcaa aaatcgaagg 94621 tagccattct gctgtatgat ctattgatac atacaggatg acatttgtcc tgtgggcacc 94681 agggggcact gtgacccctg gtttgggaca ctaatggctc caagtccctg gtagtacttg 94741 agtcatttcc tttctggccc cttctattga gtggaatcct gaaacccacc tgactcttct 94801 actcttgaaa aattacgcRg agaatgtcct cagacatagg gtcagacaag aacttcgaca 94861 tacattttaa agaatggaga gcagatttac atccgctgct taacagggaa ccaactaaca 94921 aagaattaag aataaaggaa gtagagttgc atgaagagac tcccctttct atgataagaa 94981 aggcctgaag gtcccttccc agctgtggac tcagaggcag agctctgggg catttccatt 95041 atggcctgga cccctcccct gctcgtcctc actctctgca caggtgctgc ctcccagggc 95101 tcagccccca gtgggatcaa gatcagcctg gccctgacct tcaactcaac atagggagtg 95161 atgcagggtg tggggttctg ggaatgaggc cctcatcctc agactcacct ctcctgtcct 95221 ctcttgtggg ctccgttatt tcctctgggc caactcaggt gcctgcagtg tctgtggcct 95281 tgggacaaat ggccaggatc acctgccagg gagacagcat ggaaggctct tatgaacact
95341 ggtaccagca gaagccaggc caggcccccg tgctggtcat ctatgatagc agtgaccggc
95401 cctcaaggat ccctgagcga ttctctggct ccaaatcagg caacacaacc accctgacca
95461 tcactggggc ccaggctgag gatgaggctg attattacta tcagttgata gacaaccatg
95521 ctactcaact cacagtgaca caggcagatg gaaaagtgag acacaaaccc tttttctgtc
95581 tgtgtcactc acttcctccg gccccagcag gactgtggac acagccatgg gcaggtctgg
95641 ctcagttttc ctggatctga gaccccaggg tgccctgacc tccaagccct ccagggagac
95701 tctgaagaga gtgggttagg tcaggagagg acttggggct ggcaggacca gactgtcctg
95761 gtattgtcca gagtggctgt gtcttggctt aagatgacct gagtgtaagg atagtcagag
95821 ggagacagcc attgagtggt aatggtccct ggatttcctt ctgtatggtg actggaccta
95881 aggcagtgct gcttttctgt ggcccaaatg ccccagatca ttctgaactt ctcaggttta
95941 actgagaccc tcaagcccca gctccatgac atcctcatat tctgaacagg gagggccacc
96001 acaatgggca gtgtggtgtc catcaagatt cccctttcag ggctcaccca tccRccatac
96061 acccaaagct gccgggaggc cctggcttct cacagctgct ccccaccgga aaatgccatt
96121 gattgcaaag aatggcctcc cccaaaagtt tgcccatgtc agagcgtggc tcaggtgcaa
96181 tgactgcttg atgcctcaat cccaaagtca ggccttaatt taggatggcc ttgaagtgtg
96241 ttgcagctga gcactcaatt gtaacacatt cccactcatg cctcccatac ttctttcctg
96301 agaatatatc cctaaacctc actgcatgga attccccctc tcagagtagg agtctgggga
96361 attaaaagat ttaaaaacaa accaaaacag tccaaaggga agagtaacaa agaggagaga
96421 gttgcacaga gagaggactc tggagtctgc aaatgttcgt gagcaccgat gagcacatat
96481 gtgggaggaa acaacctctg gaagagaaaa gaaccatcca aagacacttg agagaacact
96541 ccagagcgtg tgtgcacgca cacacacaca cactcacacc ccacataaag aaaacaacaa
96601 cactcatcat catcaaattg ctaaaaaaca ttttagcaat tttatgctaa aatgatgatg
96661 aagaaatgtg gtgaagaaat gtagccttaa aagtagccag agagacgagg tggctcacgc
96721 ctgtaatcca agcactttgg gaggtcgagg caggtggatc acctgaggtc aggagtttga
96781 gaccaggctg gccaacatga tgaaaccccg tctgtactaa aaatacaaaa aagttagccg
96841 gatgtggtgg caagcgtcta taatcccagc tactcgggag gctgaggcag ggagaattgc
96901 ttgaacctga gaggttgcag tgagctgaga tcgcaccact gcattccagc ctgggcaaca
96961 gagcaagact caatctcaaa aaaaaaaaaa aaaaaaaagt cagaggcaaa agacatgtta
97021 gatacaggtg aacacaatga caagagcaga atttttatta gaagcaatgc aagcaggaag
97081 tagtggagct atatttataa aatatgaaaa gaaaaaWctt ataacccaga gcaatctaca
97141 gcctcactga aatccctacc aaaataccaa tgacatacct cacagaaata gaagaaaaac
97201 cccacaattc tacaatttgt atggagccac aaaagaccca gtatagccaa aatgacactg
97261 agcaaaaaac aaaactggag gcatcacatt acctaattca aaatatgcta caaacggccg
97321 ggcgcggtgg ctcacgcctg taatcccagc
InthePRAMEgenomicnucleotidesequence,twonovel SNPswereidentified(SNPs AAandAB). GenomicsequencecontainingthenovelSNPsisprovidedbelow:
AA
GCACGTTCTAAAATGTGTTTCTACATCAGCAAAGACCGACGATCTATAATGGCAAACCTGGCACACATA[GZA]GGCATTT
TAGAGGGCTCCTGGGCAGTGTGGACATTTTCGATGTGACACTGTAGCTGGAAGGGAGTGGGA
AB
AGAAAGAGGGTAGGATAAAAGGAGTCAGCTTTATATAACTTAACATCCCTGATGCTCTTTTTTTTTTTT[- /T]CAAGAGATGTGGTAAAAGAATGCCACTACCACCCTCTCCTTTCTCAACAAAGCAATTTAGAGGGTTCCCGAATGACCT
ACATCAACAΆTTGGCCTGAACTAATCAAΆCGCATCATTCAGATTCCCTCAGTAAAATATTGAATACCAAACAG
[0380] Following is a genomic nucleotide sequence of a ZNF221 region (SEQ ID NO: 6).
>19:49113301-49207600
1 aaacctccca gtctaaatga ttaagaaagg tattaatttc ttaatcaaac aaattgttca
61 aatagaaagt tacattgtaa taatggaaac ttgggaaaca gataaatgtg tttcctttaa
121y gttgaagcct acatagtttg tcatgtctgt atatactttg caagttttca cttttatttt
181 tatttatttt ttttgagatg gagtttcaSt cttgttgccc aggctggagt gcaatggcgc 241 gctgtaggct cactgcaacc tccacctccc aggttcaagt gattctccta cctcagcccc
301 ccaagtagtt ggggttacag gtatgcgcca ccatgctcag ctaatttttg tatttttagt
361 agagacgggg tttcaccatg ttggccaggc tggtctggaa ctcctggtct caggtgatcc
421 acccacctct gcctcccaaa gtgctgggat tacaggcgtg agccaccatg actggctgca
481 agttttcact tttaaatgtg actttttttg gtcttcctga gaatgtgtca atttgaggag
541 ggcaatttcc ctattttgcc caggttataa tgtttctaat tgaaaatgat atgttaggcc
601 cttattacaa tgcagagaaa actaaaatct accttcttta gtatatgttt atttaatggc
661 tatcttctat aacatttatt tgcttagtgg ctaattttgt agaaaataaa ggcattgcca
721 tacataccat atttgaatag ggactactaa aatgataagt aatattcttt tctgtaactc
781 tctttggaag aggtggatgg ttgccacaaa gaaaattttt aaagggaaag acttaaaagc
841 tattctctaa acattgtttt ttccttgtgt aaagtgtttt tggggacctg cctaaaaMgt
901 ggctatgaga acctttattc taaaaagtag ttcttcacca agtgagcaag attcactatt
961 aacagctaca ggaaagccac aagggggaaa tgatactcaa tttgattaca actaaagcta
1021 agctttcatt agatgtgagt gttatggtct caatgtgtgt gcccttgcca tttgtatgct
1081 gaaatcctaa ctcctaaggc attaggaagg tgattatgtc atgagggcag atctttcatg
1141 aataggatta gtgcccttat aaaagaggcc tgagagagat ttcttgcccc ttccctgtaa
1201 ggacgcagca aagaaggtgc tgtcattgga tcaggaagtg ggccctcagc agacactgaa
1261 tctgctggtg ctttgaacat ggacttccca gcccccagaa atgtcagaaa taaaattccg
1321 ttgtttataa ttcccccagt cSgtagtatt ttgttttagc atcctgagca aactaagtgg
1381 gttaatgaat acagatcttc atatatccaa aagcacccaa taaattagaa ttagattaga
1441 tattcatcag ggggaaaaaa actgttctag actatgcctt cgtcacacat ttttgtttgc
1501 caaccttgga atatctccaa ggctaacaga aaaatgtcca gcagccccag cccctcctca
1561 cctgaggagt tatctctctg ggttgccatc ttcatcatcc acagcttttc ttctctctct
1621 aactgtggta ggccatctgg tgtggactga tgccctgtga aaaggcaagg acataggttt
1681 ataattaata catcagaagc caaaattact caaa'attatt gtcctcattt catcRtcttc
1741 aggacatcaa acttagtgtt ttccacactt ctaatcatga cccattggag gaagtatatt
1801 tcatgcaaag acccaagata tgcagatata agtataaatt actgggaaaa aaaatgtttt
1861 catcataccc cctccattgt ctctcacatt cttcttggca acaggacata gtttttgctg
1921 caatgtgcag ttattgccaa gtgctctgac attttctacc ccattttatt tcattaaaaa
1981 aaccaatggt acaattcatc aaatgtatat caggacccac taactggaga tgaccacaat
2041 ttgagaaagg ataaaagtgt atagttgtca tgctgtcaat ttatggttca ttaacaaaat
2101 acacactttY gccaggggag aaagacttta aaaattccat atctgtctta tgatggatga
2161 ttagaaaact gaaaaccagt tcaagagctc caaagccctg tatacccaag gacagagaga
2221 caacagaggg agccaatgtt cagtcagaga atgcctgtcc tcacccactg agaccacatt
2281 cctgaagttc tccagcatca catctcggta cagcttcctc tgggcaaggt ccagcagttg
2341 cagctcctcc tcagagaaga ccacagccac gtccttgaat gtcactgcct cctacaacat
2401 caaacacact ccacctaaat cttgcaataa aggtccactg gaaaagggat agcactgaga
2461 aatgtgaaaa agatgtgtag ggaaaggtgt cagattctta agagaccata ttcaacttta
2521 Ytacagttaa acacatataa agtgtattat attttaggtt tttccttttc ataataaatg
2581 ctgtaaagaa caactttttg catagatatt acccacctat tttcttaggt aagttccaag
2641 aagtgaaaaa tcacaatgtc aaagaatatg caacttccaa attatgatgt acgacagatt
2701 ttcttttcaa tttttccctg tcttatcctt cccccaagat aaagtatcat tgattttcca
2761 taccatagat aacactgaat ataaccaatt tttaaaaaat cctaggcaat ctgttcatta
2821 agaaacaact gggttttttg gtctgtaatt aactagtaag atcatatttt cgtatattta
2881 ctggacatct ttatttttct ttggtatctc acctgttact gtccttttat tgatattcca
2941 tttagaattt tatcttataa ctctgtaaaa tttctagtga tgattttaag tcgtttttta
3001 gcatatacgt tgttgttcac ctacattttg ctggtgtttt atagctttgt gttcaggagt
3061 gtttcttaaa ctcaacacta ttgctgtctt gggcgggata gttgtctgct gtgtgggggt
3121 gtctagggca ctgtagggca ttttgcagca cccttagctt ctacctacta gatgctggta
3181 gcacccccga cctgtggtac caggaatgtt tccagatgtt gccgggtggg gggggggggc
3241 agtgggcggt aaagtcaccc atgataaaga agcactagca tatcgtattg gttttttttt
3301 tttttttttt gagacagagc cttgctctgt tccScaggct ggagtgcagt ggtacgatct
3361 tagctcactg caatctccgc ctcctgggtt caagtgattc ttgtgcctca gcctcccgag
3421 aagctgggaa tacaggcgtg cgccactatg ccaggctaat ttttgtattt ttagtacaga
3481 tggggtttag ccatgtttgt ccaggctggt ctcgaactcc tgacctcaag ttatccacct
3541 gtctcggcct cagaaactgc tgggattaca ggtgtgagcc actgtgctcg gccagcatta
3601 tcatactgtt ttaacttttc atatgaagac tgttcatccc tggagccctg cacattcagg
3661 taccotaaat gaatgacagt ggtcatagag aagaagaggc cactggagag tgaatcgagt
3721 aggggcagta ttagctgagg catcttccac taaccaggac aagctaggca cccacacaaa
3781 aagggccagg tccactaagt ggagaattct tttYctttgc ctctctctct tcagtcaagt
3841 aaaaatcctg gagtcttttg aaatcagttg gatctttggt atcctatgtg aaactaagca
3901 actttccaca attctacagt cttgtttctt tgtaattaaa atgcacatga gaccaaggac
3961 tgcctcataa agttggtttg aggaataaat acagtaagag aagtatctgg taagtcctct
4021 tgtactgcta gacttttaaa attaagtaca ttattacttc aataaaataa gttaacataa
4081 gagtaaaaga atacggatca gtaagtaaac atacataggg tataactcag gcctgggtaa
4141 gaaaaatcta gctttctttt gtggaaatat ctaataatat tttctctaaa atcagtggca 4201 tcatgtcaca cgatgtcata tYtgaatcat tgacatagga actacccaaa gtaccacata 4261 tcaaaagtac tttttcaata tagtgtttct ataacattaa aagtaaataa tagcaaaact 4321 ccttcaaatt gctttttgga aaaaatggtt atttgtggga aaactgtagt aacaattttc 4381 agtaaaaatt ctaaactacc taaacattga tgaatgagga gaaaagttat ctcacMtaca 4441 caccacagag taacatcaat tcaagggact ataaaaatat ggagcaagta atattaatta 4501 gaaaagatgt aacttgaaca ttttgtctat acaatgctta taaatacgca gttatgaata 4561 catctgagga aaagaacatg gaaaatcaaa acagttgttt tgtaagggaa acagtaggaa 4621 aatgagagat ttttattgtt ■actgactttt ctattctatg cttatttatc aaattataaa 4681 gtcataaaat aaagcaacaa aaattaaaca acacaaaagt aaaaagcagc aagcatttgg 4741 cacacaagag gttctcaaat gtctaccgtt ctccttcatc ttcctaacaa agagaataag 4801 gtgtRtgtaa tctttccgaa atgagatgac aattattacg gagaaaggga ggtccaactc 4861 accttagact tcgtcatttt gtcctcctcc ttctggagaa gtgccaagtc ttaagaggga 4921 aggagaaaac atgagggaag gagaaaaaag ccatgagagt ttggccaaaa aaaaaaccat 4981 agctgttttt ttgttttgtt ttgttttttt taacgtgaaa tagcaacagt gttccttcct 5041 cgggctggct tgatgaacag aaactaggct ggattccaga tactacttgt ccctttggct 5101 gtaagaccct ggcagcaagt gacctgcccg accacacaga gtatgccccc aacataattt 5161 ctccttgtgg ctctcagggt atgactgatt ctagggagag tagaaattga gagtaaaata 5221 cccagattaa gagtatttgg cccctctgga gccaaatgcc tagatttatg gcattgctat 5281 ttattcgcta tgaaatccag gacaaattat taatatctct gtgcctcagt tctttcatcc 5341 ataaaatgaa gagattaaca atgagagaat taacaatctc ataggattgt ttacaaatta 5401 aatgagggaa aaagctcaaa gaatattttc agagtacctt atttcttagc tgcagaaaag 5461 cacataacat aaaacctgtg ctcattaatg gtttctacca ataaattctt tgtttttttt 5521 tttttttttt tttttgagag agggtcttgc tctgctgccc aggctggagt gcagtggcac 5581 aatcatgact cactaaagcc ttgacctcct gggctcaagc gatcctccca cctcagcctc 5641 ccaagtagct gggaccacag gtgtgcacca caatgtccag ctaatttttt tactttttgt 5701 agagacgagg tcatgctatg ttgcccgagc cagtctcaaa ctcctgagct caagtgatcc 5761 tcctgcctca gcctcccaaa gtgctgggat tacaggtgtg agccaccaca ctcagctgat 5821 gaattcttta gtagaggttt tttaacctct actttaacct cgcctttaac ctctgaaata 5881 cctgttattc tctcttggtt tcccatactt aatttgaggc ttttgcaata gtatttaaga 5941 ttacagtggt ccttcctatc catagttttg ctttctgtgg tttcagttac ctgaagtcaa 6001 ctatggccca aaaataggtg agtacagtgc agtaagatat tctgagagag agagagagac 6061 catgtttata gaacttttgt tacagtattt tatcattatt ctattttatt ataagttatt 6121 gttgttagtc tcttactgtg cctaatttat aaattaagct ttatcataag tatgtaggta 6181 aaggaaaaac atagtatatt tagggttcag taatatctga ggtttaagtc attcactggg 6241 ggacttggaa ggtatctgct gtggataagg ggggactact gtacatagat gactgctttt 6301 tttgatagta aaatatgtaa cataaaattt accattttaa ccatttttta agtggttaca 6361 ttcagtaagt acatttacat tgttgtgcag ccatcaccac tatccatcat cagaattttt 6421 tatgtaccca ttaagtaata attccccatt ccccctccca gcccctgata accacgttat 6481 ctctatgaat ttgactatat ggactggttt ctaaaaggta taagaccttg caaaatttgg 6541 ctgtgtagag tggctcatgc ctgtaatcct agcactctgg gaggccaaag tgggaggact 6601 gcttgaggcc aagagttcaa gaccaacctg gccaacagag Ygatctctaa agagaaaaaa 6661 aaaaaatcta aaaaaaaaaa aaaagacctt gcaaaattta ctttaacagt agggccttaa 6721 aaagactatc atagagacag agaaaattta ataaaaaatc atgactctgc aagatttatg 6781 caactataaa ttagataatg tcactgaaat taacaagcta taggaagcac aataataaca 6841 acaaaaatgg tgatatttaa ctaaatacat attagggacc ttatgaagaa cttggcacat 6901 gatgtttcat taaggtactt cacacatacc tgtgaagaag ctactgttgt atcagttgtg 6961 tgaccttggt ccaaacacct aaacttctgt gcctatgctc agctataaga agggaaatga 7021 aaacaggatg ttaatcatag gattactgtg aggaatcaat gagtgaattt atgcaatgcc 7081 cttggcatag tacctggcat tttagggagt tctcaacaga tgataatgac gacaattgta 7141 tccatttaac aggcgaggaa gctgaggctt ggtgaggtca agaggcagga acagtacctg 7201 aatccagctt tcataccaga gccagtattc taaatcttgt ctctctgttg cttctaaaga 7261 caaaaataag cagatgggag tctaagatct catggaagga gaaatgaggg ctccagagtt 7321 aggggtaaaa gacagacaca gaaatggaga ttggactctg aagccaaggg agctgcttga 7381 gggagtagag aacagagttg tgaacagtct agactccaga accagcccac cagtactgga 7441 atacaggcat Stccaacttg ctagtacttt gtgctctaat gtctaatcta taaactggat 7501 gtaataataa tacctacttc ataggaaggt cagtgaaagt gactaagtat acacaaaaca 7561 tgtggaacag tttctggcag cttaaacaat caatagaagt tacacataaa cccatgcaga 7621 tgctttacat gaaacagtca gggagcccct cccttgctgY tgtggaccaa tgtctgtgtc 7681 ccctcaaaat tcttatgttg aagccctaat gtgatgttaY tgggaggtgg ggcctttagg 7741 aggtaattag gtcatgaggg tgaagcctcc ataatgggat tgctttcctt gtaagaaaag 7801 caagtaacct agctctttct ctcttcctcc caggaagata caagacaaca gcttctgcga 7861 accagtaaga gtggcctcat tagacattgg atttgccagt gacttgatca tggacttccc 7921 agtctccagg actgtgagtt ataaaggttt gttgtttaag ccacccagcc tatggtattg 7981 tttgttatag cagcacaaat ggactaagRc actcacggag tctgcagctt cacatggctt 8041 gatgatgggg aaacatgggc tggattccag attccacttg acccttcagc agagagcctc 8101 tgcacagtga aaaatggagt tcttgtagct ctcagggtat gattctgatt ctatggaggg 8161 tggaaacgag taaaataccc agattaaaag cttagtatta gttaattact gcttgattta
8221 ttagtaaatt attcttagtt ctcttacttc agatagaaga acccttacta ttgaaaaggg
8281 aaacataaaa ccttccaatc ttagaagaaa tacttattta tttatttttc tgaaatggag
8341 tcttgcgctg tggcccaggc tggagtgcag tggaatgatc tcagcttact gcaacctcag
8401 ccttccgggt tcaaaagatt atcctgcctc agcctcccca gtagctggat tatgggcacc
8461 cgccaccatt cctggctaat tttttttgtt tcttttgttt ttttagtaca gatgaggttt
8521 cactattttg gccaggctgg tctcgacctc ctgacctcaa gtgatccacc cgccttggcc
8581 tcccaaagtg ctgggattat aggcgtaagc caccacaccc agcctgaaat actcttttag
8641 aatttgtaat tcactttgtt, ctaactctat atttgcccag caacctctgc tcagtaagaa
8701 tgttttgcag gggatatatt' tccatcgcct cgggaaaaaa aagagataat agcaaatcaa
8761 ccatctgtct tgtgatgaat gtttattcag ctttatatcc caagtaccta aaacagttcc
8821 tgtacaaagt aattgcccag cagaaatgac tgaacccacc atcagacatg gagaggcata
8881 taggaagact agactagttg gtctaagtct ccctgagact gactccagtt gggaagattt
8941 ggagagcaag ggttttattt ccaaattttt cctctaggtc tttcaccata tttgctgtcc
9001 ttgggcaaga tcagagtcat ctttggaagg aaaagcatct ctggattttg ttaaatacca
9061 tcttaggaaa gtactctctg ctcttcaaag tcagcgacga catgagtcac tgttacagaa
9121 cccacagaat gtgtccctta aaatactgag aatgaacact attttactgc ctgcataatt
91&1 agcactagac aggtgaattt attactactt cattaaatag atgagtttat taactcagtg
9241 aagacttgtg ggaaccttac taaaagagat gcaattcccc agcctcaaga tgctcacagt
9301 ctagtgatga ggaagctggc aattccacta tacatggcta aatgccagag tagtgcacag
9361 catcctttag cggcatgagg aatgggctca taagcccaca gtggaatgYa ggggttctac
9421 acaaacgtag cttaatctga gccctggagg gtgactgagc tctattagcc agagaatcca
9481 aatcactata aaactcccag agaacaaatg tcatgatgtg aagacaaata acaggcagaa
9541 agagatgggc aggaaggcag aattcaaacc atgatgacgt tcaagtcaca aaattatctg
9601 caagtcagca ggagaacact gaaggacttc tggaaaggag agtaaaaaga ttcctttcat
9661 gttagaaaca gattaccctc tcaaacggga ggaggcttga gggggaggag .gctagggcca
9721 gagagactat taggagagcc acactaagaa actctttctt ctttattcaa atgtcaccac
9781 ctcagagtct ctctctagcc atcctgtaga taatttcggt ctcctaactg caccccaaca
9841 cttcctatcc ctcatctctg cttacttctt ttctctaagc ccttattcct ctctaacatc
9901 cttctaggta tttacttact tatattcatt acacttgtct aatgtggagt ttcgttacct
9961 gctccatccc caacacctac taagttgcta caagcatttg gaaggaaccc aaagatttgc
10021 agaatgacta ctttaatgac tcagtgaata gttgatagat acaccatgtg gatcagggcc
10081 tcattattta tcacctgaac cattcattca acatatgatt gttgaagggt ctactctgtg
10141 ccagccaagg gtcctagatg tgaaatatac agcagtgaac aaagaccttg atctcacaaa
10201 gatgatatta taatgggcta cgagggcata gcaaaccccc aatatatagc gagagacact
10261 tttaagaaca atagggcagg gtaaagaaat aaagagatga aaggaggggc tgtcacagaa
10321 taagtttttg tcatgaaaga tttctcttat atttgaacag agacctcaaa tataggaaga
10381 gaaagccatg tagagatctg atatctgaag gcatgtaatt cagccctgaa gaaMatagaa
10441 aatttttttt tgttaaaaag ccataggtct ggtatttagc aaagacatgg aatcaaccta
10501 aatgcccgtc gatagcagtc tggataaaga aaatgtggtg catatacacc atggaatact
10561 aggcggccat aaaaaagatg tcctggctgg gcgcggtggc tcatgcctgt aatcccagca
10621 ctttgggaga gtgaggcagg cggatcacct gaggttagga gttcgagaac agcctaacca
10681 acatggagaa accctgtctc tacttaaaaa aaaaaataca aaattagcca ggcatgatgg
10741 cgaatgcctg taatcccagc tactcaggag gatgaggcag gagaattgct tgaacctggg
10801 aggtcaagct tgtggtgagc caagatcgca ccattgcact ccagtctggg caacaagagc
10861 gaaactctgt ctcaaaaaaa aaaaaaaaaa aaagatgtcc tttgcaggaa gatggatgga
10921 gctggaggac attattctta gtaaactaat acaggaacag aaaaccaaat accgcatgtt
10981 ctaactcatc agtgggagct aaatgatgag aatacatgga cacacagagg ggaacaacag
11041 acactggggt ctccatcaga gggtggaggg tgggaggagg gagaggacca gaaaaaataa
11101 ctcatgggta ctaggcttaa tacctgggtg acaaaataat ctgtacaaca aacccccatg
11161 acacaagttt acctgtataa caaacctgca cgtgtacttc tgcagttaca gaagatcaag
11221 gatgggacta agaacctatt tctaccaata tcccaggtga tgctaatgct gctggtccct
11281 aaattacaat ttgatagcat cagttaaaac agctctctag atattttcag acagactaac
11341 acttgaggtt ttcacctcca atttttctac taaccccaaY atactatcct attgcctaag
11401 ggcaaaaaga actaaagaag gtaaaaacaa actgcaagct tctctttctc tctgatacta
11461 acaaacagga atgaaggttt gttaggatca ggagtgccaa gatcaactct atgctcctaa
11521 cattaaccac gaaaaaaccc aggccactca taaaatactc cctataatga cagcaactga
11581 catttagagc aactgactgg ctgcctacca aggcactgtt tacataaatt cttcacatgt
11641 agtatctcat ttgattttca taacgatgat agagattgag aaaataatta tgcccatttt
11701 acagacaagg aaatagaggc acacagaagt aacttgttca tgtttaacaa gtggtcaaca
11761 gaaaagtcag gattttcacc cagtgcctta tctactagaa cactggctgc gccaggcaat
11821 gaacccaggc aatctgacac ctgtgcccat gttcttaacc gctcgacagt ggttctcaaa
11881 ccctggtatg catccatatc aaaaggcctg aggttccatg tgctgccttg atacctttga
11941 gactcacagg gccctgaagg cctagccatg agctcccggc tctctccaga tatgccctct
12001 cagtgggaaa ggatccctgc ccgcctagtt cctcgtgtgt taaccatgtt ttatgtactg
12061 gctatttgat atctgctatt cctaatctct ccctaagtct gctgaatcac agggccagac 12121 taacaaccaa aattatgtgc atcttccctg caagcagctt tcaccggtgg gagaaagaca 12181 tattacaaaa gcataggccc cagttccttt ctctgtctgc ttcctgaccc ttgcctgtag 12241 tgggcttcat gtggtctggt gtggtgtgat gtaccagtgg aactctaagt attaaaatac 12301 tattcattgg cattaatctc tccatgttgt cactcagccg cttttataaa attaagactt 12361 aggcacaatt catcctattt agccagacca cctgtacccc accaggtcct ccacataaca 12421 ggttttgcct ccctgccagc ctgYagaatt atccaaatgg tcaattaaat cttcttgcag 12481 gaaccaaggg tcaccctgct accctcctgt cactacaaaa tcaacctacc atggcccctg 12541 ttggttcaat ctattctcaa gtgcaagtcc cctgggctgt gaatatgtga ctaataaact 12601 gctaccaacc tcttctgcat aggctgactg tagggtttgt gtgttcagcc acctcctgcc 12661 agttcaagtg atgtcttagt ccatttttgc tgctataaca aaataccaca gttataaaga 12721 acagaaattt ctcacagttc tgaaggatgg gaagtctaag atcaaggctc cagcaggttg 12781 tgggtctggt aagggccttt ttgctgtgtt ctcacgtggc agaagggtag gggaaaaggg 12841 ggatggagga atgctgtgtc ttcatagtct tcatatggca gaagaacaag ggaggtgaac 12901 tctctctctg aagcctcttt tataagagca ttaactcctt catgccctca tgacttaatc 12961 acttcccaaa ggcctcacct cttaataaca ccacaatggg gattaagttt caatgtttgg 13021 gggacactca gacagcagca ggtgggggaa tccctccttc accaatgggg tgaataggtg 13081 accagaaccc ccgaattact aataaagaac acagaagttc aagctcatag aagtagtgta 13141 gggcctgggt cttatgattc tgatgtgacc acagttcaag aaccactgca ttattctaca 13201 ctgcctctta aagagttgac ggaactgtcc aagggcaccc aggaaaccag gggcatggct 13261 ctgcctggca ctcaggccta ctgattatca gctccctgct cctctccagt ataagatgcc 13321 cctagagctc tctataagag gaaagggctg cagaatgact ggtaatgcct ctggaggcaa 13381 attctcagaa tgaaagggga ggcttccctt agatcagtgc tgtccaacag aaatataatR 13441 caggcaagct attcgtgtaa tttaaRtttt tctcatagct acactgMaaa aggtaagatg 13501 aagcaagtga acttaatttc aacaatatat tttctttaac ccaacatatc caaagtatta 13561 tcccctcaac ttgtaatcag tataagaatt attgagggat tttactttct ttttttcata 13621 ctagatcttt gaaatctgtt gtgccttacc attataacat atctcaattc agactagtta 13681 cgtttcaggt gctcaataat cacatgYggc taatggctac actattggat agtgcaggtt 13741 tagacctttt tattaaggca atatagacta tataatcatc tcacagggac ttgctaagtt 13801 caaaagacag caaattagtt gtaggtttca acaatcacta tcatagaaac tatgcacatt 13861 tgtttaaata tgtaacagaa ttaaagtatc catagaaaaa gtggaaagtg gggaagaaaa 13921 gaatacttaa aaatatacct ttgaggctgg gcacggtggc tcacgcatgt aatcccagca 13981 ctttgggagg cccaggcggg tggatcacga ggtcaggaat tcaagaccag cctagccaag 14041 atggtgaaac cccgtctcta ttaaaaatac aaaaaaatta gctgggtgtg gtggcgggtg 14101 cctgtaatcc cagctactcg ggaggctgag gcagagaatt gcttgaccct gggaggcaga 14161 ggttgcagtg agctgagatc gtaccattgc attccagcct gggcaacaga gtaagactct 14221 gtctcaaaaa aaaaaaatgt atacatatat atatatacct ttgctccttc aataacttgt 14281 tgcatttttc aacttatctg tagaagatga aggccagctt tagtctataa tacacagagg 14341 acttactaca tgcgaggcac tatttcaaac atgttacMta tctcttttag tcttcacaga 14401 aactttacga ggtagttatt aatatcatcc ctacttaacg ggtaagaaaa ctgaggcaca 14461 gaaaggtcaa gtaacttgcc caaggttatt cagtcactga gtgttccaac ctagctgctg 14521 aggtttgatt ctggctctgc cccgctgatt gtgccaccag ttcatctgta cagttaactt 14581 gtgtgtgttt gttttttcat ctttaaaatc agaataaaat agaactgtca taaagaacaa 14641 ataagctagt gcatggaaag cactagaaat tctccaagtc ataagaaaca cccaacaaat 14701 gtcagcagtc ctcatcagaa tctccatgtg ataaatatta aacctggcca ggcatggtgg 14761 ctcatgccta taatcccagc actttgagag gcggaggcgg gcagatcact tgaggtcagg 14821 agttcaagac taacctgacc aacatgccaa aaccccgtct ctactaaaaa tacaaaaatt 14881 agctgggcat ggtggtgcac gcctgtaatc ccagctactg gggaggctga ggcaggagaa 14941 ttgctgaaac ccaggaggcg gaggttgcag tgagccaaga tcgcgccact gcactccagc 15001 ctgggtgaca gagcaagact ttgtctcaaa aaacaaacaa accaaaaaac ccaaaaaact 15061 gaaccttaat agtggtctga tttgtcttta agggagtttg ttttagatct tgacaatatt 15121 atccacaatt gttcatcaac ttttgaatac cctgaaaaag ttgatggatt ctacagccca 15181 ccaaacgtga gataacagac aaggactgtg gataatcctt ctattttgtt ctctaattgc 15241 aagctgtagc acccaaaatc cagatgcctg ttggagagct acatgaatca cttactcact 15301 gaataactcc tctttgcaaa cctcttgctg ggtactcttg agaagaatat gataatgtcc 15361 atgaaacaga ggtccctgaa gtctaatggg agagagatag gaaaccaaga gttatatgcc 15421 agtggcaagt gcaactccaa aaaagcaaag aaaatcaatc atctttcttt tcctaattgc 15481 aactctttct agctttctca cataagtagc attctcctag ctgccaggag tgaaaaacct 15541 cagagttact gttaatatct attatgggct gaattgtgtg tgcctctctc aaaacgttaa 15601 agtcctaacg ctcggtatct cagaaataac tgtctttgga aatagggcct ttaaagaggt 15661 gatgaagttg acttacagca agggagctca cgcacacaga ggggagaggc cttacaagMc 15721 accaaaactg gtgacacctt gattttggac ttttagcctc cagaactgta acaaatacat 15781 ttctgtataa tccagccaat ttgtagtatt ttcttatggc agccctggca aatgaatata 15841 attgcccctc ccttggcctt catatataaa cgattagtga ggcctagcat ggtggctcac 15901 acctggaatc ccaatacttt gggaggctga ggtgggcgga tcacttgagg tcgagtttga 15961 gaccagcctg gccaacatgg agaaacccag tctttactat aaacacaaaa attagcaggg 16021 cgtggtgacg ggcacctgta aagccagagg caggagaatt gcttgaaccc aggaggcaga 16081 ggttgcagtg agctgagatc gtaccactgc actccagcct gggcaacaga gcaagattcc 16141 atctcaaaaa aaacaaaaaa caaaaaacac aattaatgat aattattagt ttttaagcct 16201 tactgactca tcatctgggt ttgttttaaa atactcaagg gaaaaaaagt tggtgggaga 16261 ggcaataaga ttagcaagaa tggaagcagg gtgactgtac ataagagttc attatatttg 16321 ggtatgtttg gatatttcca tagtaaaaaa taagggaata acaacccact atgaatagtg 16381 tgataacaat ttcataaaac acagacaaaa acacattaaa aactgctaaa cagaaatgca 16441 ttaaaatgtt aataattatc tatatcdtag tattaaggaa atttgtaata ttttatattt 16501 ctgtattttc tacatcctgt acagagaacg tgcatctcta tgatcaaaat atgaactact 16561 aatattgctt aaaattaaat gagaattctt tcaaaatttt ctaaagtcta aactcaacct 16621 tgctttcaaa atcttctaca gtaaaattcc aacctccctc cccaacttga tctcaaatgg 16681 ctcctctgca gccagtttcc tatgattatt tatatggatc atttatctat tttatttcct 16741 aattctaaat tgggatttca aactgtgaga ccaagggtat tcagatgtaa ggaacccaag 16801 tctaggacac aaatcccact gttcttacta gcactgtgat ataggaaagt aaattaggct 16861 ctctccattt cctcatctgt ataattggtg taataacagt acccaYatca caatacccac 16921 atcacctttg taaggtgaac agaacccaca acacaaggcc tttgtgaagg ttcagtgtgt 16981 tagtttttat aaagccctca gagtgatact cagcacccaa tgtttaacta gtgtgactaa 17041 tattcatgtc acattttctc caattataca ataacggaac acttctgtag cagaaacatt 17101 tgcaaggaat gaaatacctt ctcgcactcg gaacagaaga ggaaggtgaa tgctaggtcc 17161 tgtcacagtg gggctctgct gccagaagcc aggatgctct ccttcctcct ctcccgcagc 17221 attctttcag aagtcagggc aggggaagga aaggaggctg ggagattgca cgatcttgtc 17281 tgagatcctg gaaaatccac agaggataac tccagacgcc acctgttgag tgatgcactc 17341 tttcttaaaa aacgggtcag gcatagtcca agaacagcct cctttcctgc aggaatcaga 17401 aagaagagcc aggcgcttgc tgaaaaatga atatttttta ttatgatagt tttaatatta 17461 acRgccgaca tgttgccaca ttcctgtact attccataca cattttactt gtactatatt 17521 actaattgtc aaaacaaata tataaggtta atatttcctt ttgcagaaga ggaaactaag 17581 accagaaact aagcaccgaa agattaagtt ctcacccaac gttacaccgc taggaagcKa 17641 cagagctgtg ggaggtgggg gaaggcgggt cgggccgctg agagcccagg agatcctcag 17701 acgcctcccc attcaggtcc acttccaacc aggggactcc cgaaccgccc cgaacgcagc 17761 agctccggat acagtaaggc aatcagctgc ccagccattc aactccgccc tccgctgctc 17821 gccgcagcgt cttttcctgc tgaccgaagt gcgagccctg ctgatcctac gaccaaacca 17881 taaaagttaa aacaggtcgc ggggtcccag gactcactca cttccacgag aggaatgaag 17941 gccgcgctct caacctcttg cMgcggaagt gcccgggaga gcagggagct ctggaactac 18001 atttcccagc atccccggga ggaacggaag ccgctgcccc gccccctggg cggaagtgcg 18061 ggcttcgact cccacggccg aatcttattt ctcagaatcc ccaaggggag cgggcttcgt 18121 gttctcgtcc tcaaatagtc gaagtaccac cgaacccact gagcactggg gtataggttc 18181 ctatttcccc aggggagcaa ataacaaagc cgcgctttcc cagagctgaa gtgcccccaa 18241 aagtctggac cacacgtccG agaatcctct gtagggctga gcttcccgcc agcctgcaat 18301 tttccttttg aattagcggg gggagtctaa gtcattgctt gtagagctca gcctgctttt 18361 tccttttgcc attgttccct tttatacgtg acagaagttg cgaatatgaa aaaaaaaaag 18421 gaaaatacaa aggaatgcta tgtgtgagct aaaaacagag gtaataaaag aggtggccag 18481 agccgcccag agcaacaagc cttttataat atgggtatgg attcatctgt gattaaaaat 18541 gctcaaaaaa ggagatgcaa tcatccagca ggacatgaaa caaaagcagc aaaattctcc 18601 acttttcttg cgcttagttt tctttcattc gcaggatact gaagaaagta ttatagtatt 18661 gaataggctc tatcctatat aaaatcttga aaacattcag ttcagattaa ggaatcacat 18721 cacttcttgc tctcagaatc aggaatctgt aaaggtgtct tatgatttgc caaagtgtct 18781 atgaatattc agtaaggaga gggaggtgaa gtgagggatt aagacatgaa gtgcaaaaaa 18841 tacagaaagc tatactattc acttttctcc taggtagaaa ctaaatattt ttccaagttt 18901 attatgaaag ggtaaagaag aaaaaatgga agttgactac ataggagtag ctgtaaacat 18961 ctgaacaatg ttcaatttag gctgaggcag gagaccagca gggcgtgatt ccaagatcag 19021 ataagaaacc tgctgaaacc agcaagtgat ggaaagcaac ctgtaagttc cctttgctac 19081 ctatcagtat aagacactcc caccagcgcc gtgacggttt ataaatgcca tggcaacacc 19141 tggaagttac caaccccttt tctagagatt tccgagtaac tcacccctta atttgcatgt 19201 aatgaaaagt tggtataaat atacccagcc aacagcccat atgctgctac tctgggcaca 19261 ctgcctgtgg gctagtcttg gtctgcaagg aacagtcact gagcttttaa cactgttgat 19321 tcgataaacc tgctttcttc cgctgctggc ttgctgttga attctttcct gagcaaagcc 19381 aagaaactgc cctgcatcaa tgccatttca aaatctggat aagtactctc tctactttag 19441 taagaggaag agagaaatgt aatcacccaa tggactcatc ttgcccactg cccagagaga 19501 gccaatttat caagacagga attgcaatag agaaagagtt taatacacgt agagctggct 19561 aaatgggaga ccagggtgaa gactgaactc tgccgttttt tcttttctta ctcaaattcc 19621 tatctaaggg tctggggagt caagccctcc aaaccataaa gtctcatgag aggggtttta 19681 atttaaccct atataatgtg acttactttc caacctgtca taacacctca tgacggataa 19741 gaaaggaaat caaaatactt caaccccaaa tatgtttcat tgccctatca tgaaatagcc 19801 ctgcaaagtt gtcctttgtg Ragaaaaatc tacattctgt agagaatctc ctttcctttt 19861 tccaggccat tttttctgat ccaggagaga atcaactaag agtctgacac tttttttttt 19921 ttttaagagt tttgctcttg tcatccaggc tggagtgcaa ttgtgcaatc ttagctcacc 19981 acaacccccg cgtcctgggt tcaagcgatt ctcctgcttc agcctcccaa gtagctggga 20041 ttacaggcat gcaccacaat gcccggctaa ttttgtattt ttaacagagc cagggtttct
20101 ccatgttggt caggctggtc tcgaactccc aacctcaggt gacctgcctg cctcggcctc
20161 ccaaaatact gggattacag ccgtgagcca ccgtgcctgg gctacctttt taagtctgat
20221 gagaaaaatc tacgatctct tatttctgaa gactgatacc tggaagcttc atctgcgtaa
20281 taagaacctt ggtctccaca atctcttatc ctaacccaga catttccttt ctattgattc
20341 caggacttta gataaactct ttcaaccaat tgccaatcag aaaatctttg aatctgccta
20401 taacctggaa gccccacaca cttcacatac ccctaccccc caccctgccc tccactccca
20461 accccctctg cttccagttg tcacaccttt ctggactaaa ccaacgtatg tcttgcatat
20521 attgcttgat gtcttatgtc ccactgaaat gtctaaagcc tagctgtggc ctgaccacct
20581 tgggcatatg ttctcagggt cttctgaggg ctgtgtcctg ggccatggac gcttgcattt
20641 ggctcagaat aatctcttca aatattttac agagtttgag tcttttcatt gacagaagtt
20701 ttactgttac tgaaatcagc ctccctgaaa atttRgaggc tggggttttt aaaagataag
20761 tttggtgggc agggggctag ggaatgggtg tgtttattgg ttggggatgc aattggttgg
20821 ggatgcaatc atggggtgtg gacctcttct gggtggatgt cacatgactg gtagagtcaa
20881 gagttgtggg tctgggtggg gtcgtctggt catcaaaaat gcaaaagcct gaaaagacat
20941 agcaaaaggc caatcttagg ttctgcaata gcgatgttat ttatagggat aattggggaa
21001 gttgcaaatt tgtgacctcc agaataatga ttggtaatta tttatcccta catcttagca
21061 gaattcgggt ctctctcata atcctaaact tgtggccttt tattagtgct cttgtgagca
21121 aaggctgctc ctttcccaaa caatgcaaat ctaaagacga ctaccctgaa catgtttctc
21181 ctataagtgt gtctatatga gatttaaata tgttactcaa attcagattt gagaaaatag
21241 ccttacttaa tccttggatg tcattggaca ttttacaaac tcaatttctt ttaacattat
21301 tactactaga ggtagtggta ctgctaatgt tttaaaatca actttattaa gtataatttc
21361 tattaaaaca tacccctttt aaagtataaa gttcagtaca ttcgatgaac gtgtacactt
21421 gtgtaaagcc accacactSa agatgcatat atttacctca caccccaaaa agctctcgtt
21481 agccctgcta tgattgttct atcatRtccc cagccccaag cactaatctg ctttctgtta
21541 tgaaaaatta gttgttttcc tgccgaagaa tctcatacaa atgaaatcat ataacatgta
21601 ctctgatgtg tgaagctttt ttgacacagc attttttttt ttttagttgg agtctcgctc
21661 tgtcacccag gctggagtgc agtggcgcca tctcggctca ctgcaagctc cgcctcccgg
21721 gttcacgcaa ttctcctgcc tcagctgcct gagtagctgg gactacaggc gcccactacc
21781 acgtccggct aattttttgt atttttaata gcgacggggt ttcaccgtgt taggtaSgat
21841 ggtctcagtc tcctgacctc gtgatctgcc tgcctcagcc tcccaaagtg ctgggattac
21901 aggcatgagc cactgcaccc agccgacaca gcatgttttt aaaattcatt cgggttgtac
21961 tatttgttct tactgttgag tactcttcaa tgatacggat atatcacaac ttattaatcc
22021 atttatttct tgataaacat ttggaatgct ttcaattttt tgctactatg agcaaagctg
22081 atgtggacat ttcacaacaa cgatttgtgt aaacatatgt ttttacttct gtagtttaga
22141 ttcctaatag tgaaagtgct ggcttacatt atattttata gcatcatgaa agcataaatg
22201 atgtgcagta cactgcccat aaagtatata atttgatgag acttaacata tgtacacact
22261 taagaaacca tgccacaatc aaggtaatgg acatatccat cacactcaaa acttcccttg
22321 tgccactctg taattttttt ataggcttaa ctttatcaga aattgtcaaa ttgttttcca
22381 aaagcgttgt aatttttata tttccatcaa aatgtatgaa gattccagca tctccaatct
22441 tcaccaacac ttggtgttgt cagacttttt aagtcattct gtgggtgtgt agtgcttacg
22501 gtggtttttg tttgcatttt acaggtgacc aaatattcat cacattttca tgtgcttttt
22561 ggccatttat gtattttgtt aagtgtctgc ttaaatcttg agcccatttt tttaactgcg
22621 ttagttgtct tattattcat gaattaagag ttcttttata ttttggataa aaaacctttt
22681 gtcagatatg tgtagtgaga atagtttcct ccactctggg catgtatttt tatctctaac
22741 aaaatctttt gaagagcagg tttttatttt tatgaagtcc aatttatcaa tttttttata
22801 gttagtttaa aaaaattata tctaataaat ctttgttgac tccaaagata agatttttct
22861 cctatgtttt cttctagaag ttttataagc tttagctttt atatttaggc ctgtcatcca
22921 agtaattact atttgtatag gtgtttccaa ttcatttttt ttccaattta ttttttaaaa
22981 agactcttcc cttctttaaa ttacttgaga cctttggtac aaaatcattt gaccatatat
23041 gtgtgtgggt ttatttctga cttctggact ccctatagtg ttcttttgat ttatatgtct
23101 aatttgatgc caatattatg otgttttgat tgctgcagct ttataaaaag tctagaagcc
23161 aggcaacatc cattctccaa cttttttttt tcaaggttat tttggctatt ctagatgctt
23221 tggattttca tatgaattta acaatcagct tgtcaatttc ctcaaataag catgtgattt
23281 tttttttcct gagacagagt ctcactctgt cacccaggct ggagtgcagt ggcatgatct
23341 cagctcactg taacccccac ctcctgaatt caagcgattc tctgcctcag tctcccaagt
23401 agctggggct acaggtgtgc accagcacac ccggctaatt tttgtagaga tggggttttg
23461 ccatgttggc caggctagtc ttgaactcct ggcctccagt gatccatact tcttgaactc
23521 ctatagtgct gggattacag gcatgaggca ccacacctgg ccagcctgct gaaattttaa
23581 tcgggactgc attcaattta tagatcaatt tggtaagaat tgatgactta acaatatcgt
23641 atcttccaat ccctagtcat actatattgc tccattcatt taggcctctt ttaatttctc
23701 ttggcaatat tttatagctt tcaatgtaga tgtcatgaat gtctttgatc agatttacct
23761 ttaaatagtc gtttttaaaa taaacctcat acttaaaagc tttagattta cagaaaaatt
23821 gagcaggtag tagaggaagt tcccatatat cctataccca atttcccgtt attaacttct
23881 tacgttagta tgttacattt gctagaatta atgaaccaat attgatacat tattattaac
23941 taaagtccac ggtttgttga gatttattta gttttaaact aatgtccttc ttatgtgcca 24001 gtatgccatc cagaatgtca tgttatattt agtttatcat gtctccctag gctcctcttg 24061 gttgtgatcg tttctcagac tttccttgtt tttgatgacc ttgacagttt tgaggagtgc 24121 tggtcgggtg tcctgtagaa tatccctcaa atggattttt tcctcataat caaattaagg 24181 ttatggattt tggggaagta aagtgccatt tttatcatgt cattcaaggg tacatgatca 24241 atataaccta tcactcttga cgttgacctt gatcacgtag ctgaggtagt gtttgttagg 24301 tttcctcaYc ctttttcccc ttttctcata ttgtactctt tggaaggaag. tcactatgag 24361 tagcccacat ggaggaaata gggaattata tcccatctcc ttgagggcag aatagctata 24421 aaaattatgt agaaatattt catattttca atgttactaa tattataaat aatggttttt 24481 atttttcatt tctgattgtg tatttatcta ttttgttttt taggtttgat cagtttttgc 24541 tttatgtatc ttggagctct gtttttaggt gcatacacaa ttaggattgt taggtcttgt 24601 tagtgaatgg atcctctata attgtgtaat gtttcacttt atccctggaa gttttcattg 24661 acctaaagtc taaattgtct gataataatg taaccacatc agccatacag tttttctttt 24721 tattgtggta aaatatgcat agcataaaat ttactgtttt aactgtttaa gtgaacagta 24781 cagtagtgct aagtatgttc acaatgttgt gcaactatca ctactgtcca tctccaactt 24841 tttcttcatc acatactgaa gctcMgtacc cattaaacaa taacttcaca tcctctctct 24901 ccctagcccc gagtaattgc tgttctacct tctgtttctg tgtatttgac tatgctaagt 24961 acctcgcata agtgaaatca tacaatattt gtccttttat gtctgacttc tttcacttaa 25021 cataatgtct ttaagcttca ttcatgttat cacgtgtcag aatttaattc cttaaggctg 25081 aataatattc tgttgtatgt atatactaca ttttgtttat tcatttatcc acaaacattt 25141 gagttgttgc caccttctag atattgtgaa taatgctgct atgaacattg ctatacaaat 25201 atctgttcaa gtgtctgctt tcaatcattc tgtgtataca cccagaagtg gaactgttat 25261 atcaaatggt aattttatgt tttttttttt tgaggaacta ctatatggct ttccacagtg 25321 acaacaccat tttacatttc caccagaaat gcacaaagtt tccaatttct ccacatctgt 25381 gccaacactt gttgtatcag caatcttttg attagtgct't tcatggcaca tatttttcat 25441 cactccattt tcagtctctc tatataatta tatttaaagt gagtttctta tatatagctt 25501 atagttgggt cacttttttt cgttgttgtt gtttgtttgt ttatttgttt ttgagacgga 25561 gtctggctct gtcgcccagg ctggagtgca atggcgcaat ctcggctcac tgcaagctcc 25621 gcctcccggg ttcatgccat tctcctgcct cagtctcccg agtagctggg actacaggcg 25681 cccgccacca cgccgggcta attttttgta tttttagtag agacggggtt tcaccgcgtt 25741 agccaggatg gtctcgatct ccaggcctcg tgatccgccc gcctcggcct cccaaagtgc 25801 tgggattaca ggtgtgagcc accatgcctg atcgggtcac gtttttaaaa taaataatgt 25861 gacaaacatt gtctttatgt agttagacac ttcacattta atgtaattat ttatatggtt 25921 tgatttatac ctaccaattt attatttttg tgtttgtctc tttttgttcc tatgttaccY 25981 cctttctttc ttcttttgga ttatttgaat ttttttaatg ctctattttt atttgctttt 26041 tttttttttt tgagacaaga gtctcactct gttgcccagg ctgtacatgt tatttttaaa 26101 ctacatgact ttggttttat cttttgtttt gtttgattta gtgttgcttt agaaacccat 26161 gtatacatac ctaacatatt gtggactatt ttgagccaat agtttatggc ttcacataaa 26221 acaaggaaaa cttacaacta ttgagatctg tttatcctcc cctctttgtg ctatagtgtg 26281 tgtattatat ctacatgctc tgaaaaatcc caccaatact ttaaattttt atattcaacc 26341 atcatatata ttttaaagaa tttaaaaggg ggggaaatat tgtattatgt gtaccaagtt 26401 tactagttct gtttctctgc cttcattcct gaaactttcc tctgatatta tttctcttca 26461 gcatttcttt taaagtatat ctgctagtga tgaattctct tagttttctt ttgtctgaga 26521 aggtccttat tttatttcca tttctggagg atattttcct tgaatatagt gtctggattg 26581 acaRttcttt actttcagca cattaaaatg ctgttttact gtcttctgac atccagggtt 26641 tctgataaga aatttgcatt catttgaaat gttgttgccc tatttgcaat gtataatttt 26701 tctttaaacg ctttcaaaca tttttttctt tatctttgat attttaatag tttcattatg 26761 atgtgctggg gtatggtttt cttcattttt aacatattta ggttttgctg agtttttttt 26821 caatctgcag atgtatttct tttattaaat tggtgaagta ttcgactata atttatttga 26881 aaagtttcct atttcaatac ctttctcctc cYcttctgga attccaatca tgggaatgtt 26941 agatctttta atgttgtccc aaatgtcctt agggctcaat tcagtttttc tatttttgct 27001 ttttctcctc cagattcgat attttatact gttcagtttt taagctcaat gagttgcctt 27061 tgtcatctcc attctgctac tgactgattt taaatttcag ttattgtatt ttgaagttct 27121 gaaatttcca tttgcttgct tttatagttt ctagttttct gacaactttt atatttccat 27181 ttatttcaaa tgcttaccta tacctcatgg agaatgatta caataacttt taaaagtctc 27241 tgataatgca aaaatctaga acattcaggg gttggaatct cttgaatggc ttttttcttg 27301 aagttggtca gatatacctg tttttctttt tacatgtcaa gtaattttgg attgtatcct 27361 gagcattttc aatatgttac cagattctgg tcctattaaa atctatagag aacattgagg 27421 gttttttKtt tgtttgtttt agcaggcagc caaccaggtt aatttccttt tttttttttt 27481 tttttttttt tttttttttc agatggagcc ttgctttgtc acccaggctg gagtgtagtg 27541 gcgtgatctc agctcactgc agcctctgcc tcctgggttc aagcaattct tctgcctcag 27601 cctctcaaat aggtgggatc acaggcacat gccaccacac ctggctaatt tttgtatttt 27661 tagtagagat ggggtttcac catgttggcc aggctggtct caaaatcctg atctcaaagt 27721 gatctgccca Ygtcagtcac ccaaagtgct gggattacag gcgtgagcca ctgctcctgt 27781 acttaatttc atatcataag ttttgtctca tattctctat gtgttagttc taaaaacttt 27841 gctgtgctRt ttgggtatgc cactcagtgc gccactcagg gattagctga tttctgagca 27901 atggtttata acatagttca gttctcaatg tattttctat gttcctttgg gtgtgttatt 27961 tgaatgtgca gcttagtgat gagcctttaa attgtgtcag ttcatccaca aaactgaggt
28021 atccccttct ccagctctgt cttctgaatc ttctctcaca ctctctggat tctaagggct
28081 ttcttcccaa gatcctttga cccaaaagat gagattctct cagagtttta gcctcccacc
28141 cYgtcaccct tggcttactc tggtgagaga aaagagaagt atatgataac tatccctccc
28201 cacattttga acaacagaaa cctctttttt ttcagtttct ctatgtagac acatacattt
28261 tcactcagga ttttacatgc ccatatgcac taatattagt gcagttccac catcagtgct
28321 ggcattggga agggccagga aaaaaaaaag agatgaaagg gagtttcccc cRtacattct
28381 gactcacaga ggccctcttt ctagttttct ggccagaaag atgagtttct tttagaattg
28441 tttcaagagg aatgcttcct ggatttgccc atttggtatg atgttggctg tgggtttgtc
28501 ataggtggct cttattattt tgtttcttca atagctagtt tgacagtttt taacttgaag
28561 cagtgttatt ttattaaaag ccttttctgc atctattgag ataattttgt ggtttttgtc
28621 tttagttctg ttatgttaat gaatcacatt tattgatttg cgtatgttga accaaacttc
28681 catcccagga ataaatcctg cttgattctg atggataagc tttttgatgt gctgctagat
28741 ttggtttgcc agtattttgt tgaggatttt tgcattgata gtcatcaagg gtattggcct
28801 gaagttttcc ttttttgttg tgtctctact aggttgtggt atcaggagga tgctggtctt
28861 atagaatgag ttagggaaga gtccctcctc ctgtttcttg aaatagtttc agtaggaatg
28921 ctaccagctc ttctttgtgc atctggtaga atctttggct atgaatctgt tggtccaggg
28981 cttttatttt ggtaggctat ttattactga ttcaattttg aagcttgtta ttggtctgtt
29041 cagggattca gttttttcct ggttcggtct tgggaggatg tatatgtcca ggaatttatg
29101 catttcttct agattttcta ttttatatgc atagaatatt cataatattc tctgatgatt
29161 atttgtattt ctgtgaggtc agtggtaata ttctccttgt catttctgat tctgtttatt
29221 tgaatcttct cccttttctt ctttatgagc ccagctaaca atcttttttt tttttttttt
29281 tttttttttt tttaaaaaac cacctcctgg attcgttgat attttgaatg atttcttatg
29341 tctcagtctc cttcagtcat ctctgattct ggttattttt gtcttctgct agctttgggt
29401 ttggtttgtg tttggttctt tagttctttt agttgtgacc ttaggctgtc aaattgagat
29461 ctttgtaact tcttgatgtg ggtgtttagt gctataaatt tccctcttaa cactgcctta
29521 gctgtgtccc agggattctg gtatgttgtg tgtttgttct cattagtttc aaagaacttc
29581 tttatttttg ctttaacttt attatttacc caaaagtcat tcaggagcag gttaattttc
29641 atgtaattgc atggttttca gcaatttttt aaagtcttga tgtctatttt tactgtgtga
29701 tggtccaaga gtgtgtttgg tatgagttcc attaatttgc atttgctaag gattgtcgta
29761 tgtctgattg tgtggttaat tttagagtat gtgccacgtg gctatgagaa gaatgtatat
29821 tctcttgttt tggggtggag agttctgtac aggtctatta tatccatttg gtccaatttt
29881 gagtttaggt cctgaacata tctgttaatt ttctacctca ataaagtttg taatactgtc
29941 agtagggtgt tgtagtctcc cactattatt gtgtgggagc ctaagtctct ttgaaggtct
30001 ctaagaactt gctttatgaa tctgggtact cctgtgttgg gtgcatatat gtttagcata
30061 gttaggtctt cttgttgaat tgaactcttt accattatgt aatgcatttc tttgtcattt
30121 ttgatttttt ttttggttta aagtctgttt tgtctgaaat taggattgca acctctactt
30181 tttcctgttt tccatttgct tggtagattt ctcttcatcc ttttattttg atccgtgggt
30241 gtcactgcat gttagatggg tctcttgagg acagcatacc aatgggtctt ggttctttat
30301 ccagattgcc actctgtgcc ttttaattgg agaatttagc ccattacatt caaggttagt
30361 attgatatgt ttggatttga tcctgtcatc atgatgttag ctggttatta tgcagacttg
30421 tttgtgtggt tgctttatag tgtcactggt ctgtgtactt ctgtgtgttt ttgcagtggc
30481 tggtaatatt atttcctttc catattttgt gcttccttca ggaactcttg taaggcagat
30541 ctggtggtag caaattccct cagcatttgc ttttctgaaa aggatcttat ttctccttca
30601 cttatgaagc ttagtttggc cagatttgaa atcctggctt ggaatttctt ttctttaaga
30661 atgctgaata ttgactcccc atctcttttg gcttgtaagc tttctgctga ggttcactgt
30721 tagtttgatg ggcttccctt tgtaggtaac ctgtcctttt tctctagctg cctttaacat
30781 tttttttctt tcatttcgac cgtggagaat cttttggtta tgtgtcttgt gggtgatctt
30841 cttgtggaag tatcttccag tggttctctg cattccctga atttaaatgt tgacctctct
30901 agctaggtgg agaatttgtc gtgggtgata tcatgaagta tgtggtctaa gattctccac
30961 atctctttca ggaacaccaa tgggttgtag atttggtctc tttacataac cccatatttc
31021 ttggaggttt tgttcattcc ttttcattaa tttttctcta tttttgtctg actgttttac
31081 ttcagaaagc cagtcttcaa gctccaagat ttttttcttc agcttggtct attctgctat
31141 taatacttga gattgcacta tgaaattctt gtagtatatt tttcagctct atcaggttgg
31201 ccacattctt ttctgtagtg gctattttgt ctgtcagctc ctgtatcatt ttattgtgat
31261 tcttagcttc tttggattgg gtttcaatgt actcctgcat ctcaatgatc ttcattccta
31321 tccatatccc aaattctatt tccatcattt cagccatctc agctctgttc agacccttcc
31381 tgaagagcta gcatggttat ttggaagaaa gaagttactc tggctttttg aattgacaag
31441 agttcttgag ctggttattt ctcatcttca tgggatgatg ttcttcaatc tttgaacttg
31501 ctttcctttg gattattttt ttccttttat tctatttgat atccttgagg gtttgatgtg
31561 gtataaggtg agttcagctg actggcttca tttaaggaag attttagggg gccaatgctc
31621 agctctcaac tcctggactg catgttgtaa ctctggtggg cttatatcgg gccctgactt
31681 tgttctctgg attctcgagg ttgggaacga actacactgg ggtggccaag gtgttcccag
31741 gctgctggtc actgcactac aatggatggt gccaaagcat tttttagtgc agtggcagca
31801 ggatttgtca tcattctcac atgccagctg cagtggcagt ggcaacatgg tggggtgcat
31861 gctcatcagc tgtggcagct tgctagtggg taccgggatg cctgcttctg tgtgagcact 31921 tacagcagtg gtggaggcag catggctcaa gggggcaggg gggcccccca ctggtgactg
31981 tatgtgtagt agtgctggtg gtggtgttag catggggatg aggcactggc ttctgcaggt
32041 ctgtgtgtgt cccctgtgca tgttcatatg ttatttaacc tacactccat atttaaattt
32101 attctgtttt taatttaaat tccttgtaat tgatttgcct ccagtttagc ccacttttat
32161 gaattgtttt taactgtcac gtttctcctg tctccattaa tctggcatga ttcttcagct
32221 ttctcttttt gctcatggtg tttttgatac tattttaatg tatggatata ctgcattttc
32281 tatatcaata gtaatcaaaa gtaatgccat ttttaatagc cacacataaa gtcaaatacc
32341 taggaattaa ccaaaaaagt taaagttgtc tataataaaa actataaaac actgacaaaa
32401 aaattgaaga ggacaccaaa aaatggaaaa atgttccatg tttatggatt ggaagaatca
32461 gtaacgttaa aatatccata ctacccaagc aatctacaga ttcagtgcaa tctctgttaa
32521 aaataccaat gacattcttc agagaaatta aaaagcctca aatttatatg gaaccacaaa
32581 aaaacccaga ataattgagg ctatcctgac caaaaagaac aaaaccgact tcaaattata
32641 ctacagagct ataataacca aaacagcata gtactggcat aaaaacagac acagagacca
32701 atggaacaga acagagaaca gaaacaaatc cacacaccta cggataacta atttttgaca
32761 aatgtgctga gaacacatga ggaaaagaca gtctcttcaa taaatgatgc tgggaaaact
32821 ggatatccat atgcagaaga atgaaactag acctctattt cttaccacat aaaaaagcaa
32881 atcagaatgg attaaaaact taaatctaag acctcaatct ataaaactaa taaaagaaaa
32941 ctttgggcca ggcgcggtgg ctcacacctg taatcctagc actttgggag gctgaagtgg
33001 gtggatcacg gggtcaggag atcaagacca tcctagctaa cacggtgaaa acctgtctct
33061 actaaaaata caaaaaatta gcctggcgtg gtggcaggtg cctgtagtcc cagctacttg
33121 gaaggctgag gcaggagaat ggtatgaacc caggaggcag agtttgcagt gagccgagat
33181 catgccactg cactccagcc tgggtgaaag agcgagactc cacctcaaaa aaaaaaaaaa
33241 aaagaaattt tcaagaaaaa agaattattt tacaaatgta taaacaatat ttgaaaaatg
33301 agggaaaagg ccaaaaagcc attagtacag taggcaaaag gtttgaacaa tttaccaata
33361 ataataataa taataataat catggccttt gaaaatatga aaagatgttc tatttcattt
33421 acaatacagg aaatgtaaat taaaattaca tcggcatact atcagtttta gcctcacatc
33481 atcaggttat actaaacatt ttcctctttt caKttctcta ctgatcctca agcagtaatt
33541 tcttgtcagt tccttgaaga ttttatctcc acctttgttc actctccagt aatacttctg
33601 taataattgg ggatatttca atcaggaaat cattgtttat gtaatatttt tgcttgaaga
33661 ctcaagagaa aggtaaggac gacactcagt gcaggagtcc atattaccac ataggcgttt
33721 cggatggggg attcccttct ccggggactt ggaagaagtg tggtcaagaa gctatgtgta
33781 gttgcaacac ttccccctgg agggtaaaac tgtgaacgtt tactccttgg gcattctggg
33841 aagtgtccca acggtcggtg gggttgcggg cacttccggt caggcaggcg gatccgggga
33901 attctgggaa atgtagtcca gacgctctgt ggagtcgcgg gagctacggc tgcgggagtt
33961 accctttatt ctgtgatatt ttgagctgtt ctgcgtctcg caggcccttc tgaatttcct
34021 ggacctactc tcggaaccct caacgagcta gcgatagatt tagaggaaag gaagcaccgt
34081 cggaaagcaa aggtttggaa gggtgagggc ggcggtttgc gatccccgcg gcagagcctt
34141 cttgcgttta gggctgtcct agcatcccga gggactcggt cctgtcacgt ttcgccctat
34201 ttgggggttt ctggggctct cagctcgccc aatccgcatc cctccactac tggcgcgggg
34261 ccgattcccc cggtacaacg ttggggtgtt acttaatctc cctgggactc tttttgcttt
34321 tctttaaatg ggggatatta agaccttcct aatagaattg tcttgggaat tgaaagaggt
34381 aatataaaag agtcttaaaa cagtttttgt tatagtgaat ggcgatctct tgttaatttc
34441 ctgagaaatg gtcaggtccc tgggctggtg tcagagctcc agctggctct tctctctgat
34501 ccctgcagaa cgctggaaaa ttctggacag tctttgacct cttttttttt tttttttttt
34561 tttttttttt tttttttttt tttgagacgg agtctcgctc tgtcgcccag gctggagtgc
34621 agtggcgcga tctcggctca ctgcaggctc cgcctcccgg gttcacgcca ttctcctgcc
34681 tcagcctccc gagtagctgg gactacaggc gcccgctacc acgcccggct aattttttgt
34741 atttttagta gagacggggt ttcaccgtgt tagccaggat ggtctcgatc tcctgacctc
34801 gtgatccgcc cgcctcggcc tcccaaagtg ctgggattac aggcgtgagc caccgcgccc
34861 ggccgtcttt gacctcttat ttaggaaaag gaggggtatc tccaatttcc acagaaggag
34921 gggagggtct tgaccgtttt gtcaagagtg gtgacacaac catggtattt tgtagcattg
34981 tttccgcatc ctgacttctc taaaaggatt gcttggaggt agacggagtg actccttgac
35041 tactgacaac aaagctccac gcgagtgtga cttgtcattt ttcttttaca gtaacgttca
35101 catctattgt aggatgctgt aatcgggcat gggcattgat aagaaagtct taattgctat
35161 cttcaattct aagggcttta tacaaagaaa ttcacgtaca ccacatggta gStctgtgga
35221 gaggtactgc catttcgtct attaaagatg agagtgaaac aaagggagca ggagttattt
35281 gcccatagtt gcccaagtaa taagaagtgg agccggggtt ggaacctttg cggtccaggt
35341 cagagcccaa gctgataatt tctgggatac attctcactg aggcagagga actagctgga
35401 atcaggtggg gaaagcaagt taggacttga ttggagggac cttgagagca aggctcatgg
35461 gcttggacct ctctaggact cttttaaaac tttRtcaact attttttttc acttattaca
35521 catattttgt attcttatag aaccatatca ttatggagca ttcagaaatt gcagaaatac
35581 gacaagaaaa aaagcttaat tacctctgaa tgcaggatat aaagatagta accattaagc
35641 ttatttttat ttatttaatt cattttaaaa ttttctgaga cagagtctca ctctcatgcc
35701 caggatggag tgcagtggca tgacctcagc tcactgcaac ctccacttcc cgggttcaag
35761 cgattttcct cccttagcct cccaagtagc tgggattata ggcacacacc accccatctt
35821 ttagtaaaga cgagttttca ccgtgttggc caggctagtc tcgaactcct gaccttaggt 35881 gatctgccca cctcagcctc ccaaagtgca gggattacag gtgtgagcca ctgtgcccag
35941 tcattaagct tttttttttt ttttaatgct tattgcctta ttacaaagga tattacaaaa
36001 gctacaggtg aagagatgca tagggcaagg catgtgggaa gggatgcaga gcttccatac
36061 cttcctgggt atgccactct ccaggaacct ccatgcattc agcaatccag aagctctcag
36121 aatccagtcc tcttggtttt ttatggaaac ttcattgcat agacacgatt gattaaacca
36181 ttggccactg gtgatcagct taacctttag cccttctccc ttcccagagg ttggggagtg
36241 gggctgaaag tcccatgcct ttactctcta accatgcatt ggtctttcag gtgaccagcc
36301 acatcctgaa actacctagg ggttgccagc catcactcaa ttcatgagta tacaaaagat
36361 caatttggag attctaagga ttttagttgt atgccaggag acatgttagt gaccaaatat
36421 gcatttaggt tatcaccgta catacccatc tttgaaccca gatcctctta cataaaaagg
36481 atttacaact cagccaggcg tggtggctca cacctgtaat cccagcactt ttgggaggct
36541 gaggtgggtg gatcacctga ggtcaggagt tcgagaccag cctggctaac atggggaaac
36601 cccatctcta caaaaaatag aaaattagcc aggtatggtg gcacatgcct gtagtcccag
36661 ctactcggga ggctgagaca ggagaaacac ctgaacctgg gaggcaaagg ctgcagtgag
36721 caaagattgc cccactgtac tccagcctgg gcgagacaga gtgagactcc gtctcaaaaa
36781 aaaaaaaaaa aaaaaaagga tatgcaactc aaaagatacc accacattat cagaatccca
36841 tttagtcaca ttaataagtc cagtccagtg ggtgaagcca ctcaggtttg ctggcttcca
36901 tttgatatac ggttctaaaa gtaggagtgg tcttagcaaa acatatagct tcaccatttc
36961 aggcatctgg tgtaattgag ctaagagaca gtgtcatctt aaggtattaa tatactactg
37021 gagttctctc aattcataac tcatttattc attcctttac cctcagctac cgtttctcct
37081 tctttccatt aatacacaga ctttttcacc tttggaaggg acattagaat caccactgtg
37141 ccggtctaga ctgcagacag caatactagt ctagcaaatg cttccccctc agttcacctc
37201 cattcagata gggtaaggtt acttaggtgc agaatagtgg actgttttta ccatcaggaa
37261 atacagttat attcactgtt agcccaattt tgccagatgg ggtgaagg-ca caacccatcc
37321 ccatcgggcc ctcaggaatt ctgacacaag ttttagaaat ttagttatgg tttcttgctt
37381 aggaatcatc cctgcttcca acacttgtag ttgtagccct gatcctgtga ccactacatc
37441 aggtatgggg aaaaaaattg aggtaatatg gggaagaagg aaaaaaaaaa gtgtaatcat
37501 tatactggtg ttctcctccc ttggcaagaa ttgcatagtc atcccagcct cctttcccac
37561 caggtcttcc ccagatcaac cagaaaaata gagaaattgc tatcccactg acaYtgggta
37621 tgagcacaca cttgcgaagg tgtgtaagcc agccctgatg cttttatctc .tttgttttag
37681 acaatcaatg ttttaattgc ctgttccact tccctatcaa actattactc tgaggaggat
37741 cgctctctat tcattgctgg acattgtggg ctctatatct agtgtgtttc ttggtcttct
37801 ttcttctttt tttttaaWaa cactctgaac atttggatct tccaccatat aagcaaaacc
37861 cggtcaagaa tcagtgtcta ctgctgtcaa gacccatctg tagcctccca gggctaccag
37921 catcacttgc cagctacact caggtccttc ccaccaggga atctgtttca cagccatctt
37981 cactgtgtca tttttgctga caaacagaac agttttcatc tgcattttgt gcctcagagg
38041 gtgcaagaac atgtctcgat ttagcccatc tttgcactgc tgcactactc ccatatccac
38101 taatttcacg gacccaggca gccactgcaa gggagcacat ggggatatct gcttgttgat
38161 tcaaaacctg aagggggctc tgccaatgag catcaacagg tcctacttta atgcacaccc
38221 cctacttcaa atttctgtaa gttcatgcct cacatgggca tcccctttaa tagaccagat
38281 ctccactgct cttctgcctg agtgtatggc caggccattt gccactgccc atgagtcagt
38341 aaagatccca aagtgggttt ctaccaccat tcagtttttc atcactgcta ggaaaacagc
38401 gtgtaattca gtgcattgag ctgatatgtt tttaccttct ttgatcaggg tggcatcctt
38461 ccaaatcata tgttcattca ctttggaact gccattcaca aaatgagcag ctctttgctg
38521 gtcagttgag agttgtttat agcttctcac acagttccag agtcagtctc caggtagcat
38581 gactctgtat aaactatttc cattttatta tggaactctt ctgggcactg ccatccccat
38641 tagaatgttt tcctaacatt acctaagaga gcataggtat ttcaggtgtc aagattttta
38701 atgtctttca gtcatagggc tagcctcagt taatgcccca tagtggggta gtaaatactc
38761 ttcaagtcaa aattttctag tccaaagtct cagtaattgt cactagaagg cactcacagg
38821 cttctgtcat aagccccagc ctacattcca catggagcta tcaaaacaga atttgtccat
38881 accagcactt cagattctat tctacactgt gcggactcag gcaatcttac tggttcccat
38941 ttagcatgtc caactaatat tgcttgaagg gtagatttat atgccttctg ttttRtaatg
39001 ctagtcagga gaaacgttcc ctagtcagat acaatgtcca ttcccataaa acatttaggt
39061 aaagaagata caacttctta atataaagtc tgtttaaata gtccaacttt cataatccta
39121 tcaaccctta catttttatg ttctagtccc aggaagtttt ccaccacttg cccaccccaa
39181 cactcccccc agccatttta tcctctctta tgcgaaacac tgagagtcag caggagggga
39241 ttgagccaaa ggaccctggc tcttgtcaat ctttatcttg actaatctac ttgactactg
39301 cccccaagca attccaagtg gggtttctca tcataatctt taccttcggc cttttaaaat
39361 ttctccaaac tggagtaaat acaacaaact tatttgaaac cctttaatat tgggggatca
39421 gcagaggatc caccaaactt tcaatattat tgtgttcaga cctttgtttt agcctcatca
39481 atttccattt tattttttcc atttcttaat aaaactgtta aaagtaaaaa atcttaaata
39541 agtcaaacaa tttaattgag caaaaaacaa tttgcaaatc agacagcccc tgagctagaa
39601 tagattcaga gaagcttcac tgctaccatg tggttgaaga agatttgtaa acagaaaaag
39661 gaacgtgaca ttcaaaaagt ggaagtgagg aacagaaaca gccagtttgg ttacagctca
39721 gcttttgcct tatttgatcc ttgtttgaac agttggctcc ctttgattgg tcaaaactca
39781 gtgactggca caagagtagg ttacagtctg tttatacttc cagttaggtt acagttcgtt 39841 atatatggat aaaccttttc ttatttttat ttttattttt tatttttttt gagatggagt 39901 cctgctctgt cgcccaggct ggagcgtagt ggagcaatct tggctcactg caacctctgc 39961 ctcctgggtt caagtgattc tcctgcctca gccttccaag tagctgggat tacaggcaca 40021 caccaccaca cctggctaat ttttagtaga gacggggttt taccatgttg gccaggctgg 40081 tcttgaactc ctgacctcag gtgatctacc caccttggcc tctcaaagtg ctggtattac 40141 aggcatgagc caccataacc cgcctggaga aacctttata cctaacttaa agtatgtaag 40201 aaagcagctt taggctaaac ttaatttaac ataatgatct aaagattttc accctgttgg 40261 aatgagtccc atgactctct ctctttcttc ttagtttcac ctttatcatt gttttttaaa 40321 tgacctttaa aaaatttcta gctttctggg atgagtcgtt tgactctgcc ctctgccttt 40381 ttcctcttgc taattaacct aatgttttta ttaacatctt taatatcttt aaagggaagc 40441 tgagacagca aatctgataa ggcttctagg actgttgttc agttttgcaa gagttaagat 40501 gtcaactaat aaataaaatc aatactgagc cacaaacaaa agaaaagatg ttttaagtgg 40561 ggtcttgtga attgcaatca agaagtacag atctagcagt cagctaaatc atttcctgtt 40621 ctatccaggc aaggttgggc aggggcttat aaatgtttac tgtaagttta catatctgga 40681 aggtattagt acagtccatg attgatgtca gttggttagc aacttaggat gtctttggtt 40741 gatgatcaat ctagttcagc atagctgttt ccccaagagt ttcgcaatca ggccatgtat 40801 aaacaattcc aatcaaatgc agctggtttt acaatttggc ccagttaaac aggttaaaat 40861 ccatctggat gtgtacataa ctgaggtcca actccacatg cctcttttta gaaacttctg 40921 acataaccat ctcσattttg agttttcttt ttacaaaagt cacgtggggt atccatgcga 40981 aaggggcccc cttagccaca gcatttacca tgacctgggt aagaagcata ttcagcagat 41041 gaatatccca gtcatcataa attcagtccc acgtggcttg catgcggtat atcagctgca 41101 tcatctgggg tatcccaccg agcatttata gggtgagttg agcagtccgc tttttcaggg 41161 caagcagaca ttaccatgtc ttttatccag ttgatcaggc tggctgttcc cttgggaaca 41221 atctgttaac attgatagtg agattcaggc aatatggtta aatattgagt ttattcaaac 41281 acaaaatttt aagatgtcca cctgggagga cacggacttc aaaagattgg ataagtgctc 41341 tgaagtggaa aggtttgaga gtcattatgt aggcagggtc taggaagctt aacaggatgt 41401 cagcattttc tatacaagat taatgcatag ttatagcaat tcgatagcag tgtttccttt 41461 tattgggaag aacgtattta acattctgca ttagagatgt aatagtcaaa ggggtctttt 41521 atctcagaca aagaatcttt tatcttagat gctatctggt ctgagccagg aacaagaaaa 41581 caaagaagga agttaagagg tcaagctcag tgactcagtt tccttcgggc ctaacaactt 41641 ttagaagccc aaaatagata agactattgg gtttttggtt gttttttttt tttcacatct 41701 cctgtgtgtc tgcatcaggt gtttaccgtc tgtaattgtt gcatcatgta tcaccatctg 41761 taattgtttc atagtgaact gtgggtccta cataaaccca aatatgctct tcaatgcaac 41821 atttaaaacc aaagatgctg ctacttagtt tctctcacaa tccagttgag taaagWttcc 41881 ttaagaagct acaaaataaa caattccttc acactgtaca ctgtagtttc agtttcttgg 41941 ttttaccctt cccatacact gattgccttt tggtaaccac aggtcttaga ggtacttact 42001 gttgtcctgg tRtatttttt cccttaggga cagctttgag gcaagtggct aaagcttaga 42061 ttcactaaaa ttcgaacttg gtcaagcatc agagtccaac ccagtccttt taattacatt 42121 tgatatagtt tggatgtttg gtccctccat atctccagtg ttggaggtgg ggcctagtgg 42181 gaggtgttgg atcctggggg tgggtccKtc ataaatggct tagtgctatc cccttggtga 42241 ttagtgagtt cctgctcagt tagttcatgt gagatctgRt tgtttaaaag aatttgggtc 42301 ctccaatttc tctctcttgt tcctgctctc actttgtgac atgcctgctc ctacttcacc 42361 ttctcccatg agtgaaagct ccctgaggcc tcaccagaag cKgagcacat gccagcacca 42421 catttccttt acagtctgaa taaatgtaag ccagtgaaac ctcttttctt tataaattac 42481 ccagcctcag gtattttgtt acagtgacac agaaatgaac taatacagaa aattggtact 42541 gaggtgcaat gcattgctat aaagatgcct gaaaatgtga aagtggctct ggaactggct 42601 aatgggcaga ggttggaaaa agtttggagg gctcagaaga agataagaag atgagggaaa 42661 acttggaact tttttttttt taactttttc actgagccat gggaacttct tacagactga 42721 ttaaatagct gtgaccaaaa tgctggtggg aatatggaca atgaagtcca ggctgatgag 42781 atctcagatg gaaatgagga agttattggg aactggagta aaggtcaccc atgttatgtc 42841 ctagcaaaga actttgYtgc tttgtgttca tgccctaggg atttctggaa gcttgaactt 42901 aagagtgata acttagggta cctggcaaaa gaaatttctg agcagcaaag cattcaagaa 42961 gtggcctggc tgcctcactg cttcaaacag cctacaatta tatatgggag caaaaaaatg 43021 acttaaagtt ggaatttata tttaaaaggt aagcagagta taaaagtttg gaaaatttgc 43081 agcctggcca tttagtagag aaagaaaaag catttttagg agaggaatac atgtcggctg 43141 tggagcaacc actYgcaaaa gagatttgca tgactaacag aaagccaagt ggtaatatct 43201 gagacaatgg gaaaaagacc tggaagacat ttcagaagtc ttcaggaaag tccctcccat 43261 cacaggccta ggggcctagg agaaaagaat ggttccaagg gccaggccca gggaccccct 43321 gccctgagca gcctcaagac attgtgccct gaatccggcc actctggctc cagcctcagc 43381 ttagagtgcc ccaggcgcag gctgggttgc agctccagag ggttcaagcc ttgatagttg 43441 tggtagtttc cacattgtgt taaggctgca gatgctcaga atgcaagcat gaaggaggct 43501 tggcagcttc tacctagatt tcaaaggatg tatgagaaag cctgggatcc cagtcagaag 43561 cctgccacag tgggggagcc ccaacagaga acctctacca gggcagtgcc aaggagaaat 43621 gtgagggctc cccacagagt ccccactagg gcactaccta atggatctgt ggtaagggga 43681 ccactgccct ctgagaagtg gtcctgagac cactgctcag tcatatcact tggctgccca 43741 aggccttggg agtctatctc ttataccagg atgtgggaca tggagtcaag gattatgttg 43801 gagctttaag agccctggct ggagtacagt agcacaatcc taacttgctg cagcctttaa 43861 ctcctagact caagctatcc tcctgcctca gcctcccaag tagctgggat tacaggctca 43921 agccacagca cctgcctctt catatgctct ttacccaaat tttccaatta tttacatttt 43981 gtattttgct ttgttaacac ccatcagcca aataccactg ggaacaaact tataatgaga 44041 aacaacttat tgtagctttc tgcatcaagg aaScccacat agcaaggtga tcatggagtg 44101 tcttggtaag aggcatttag gaagggctgg ctatagagtt tcatataaga tgctaggtag 44161 tattagatat aatattaagt aatatcacta ttaaatacta gatatcaatt aaaagactat 44221 taatattagt taattctgtt acctattaga tatggctaga caccatcatc ttttctatcc 44281 aaactcccat atttaaattt gttgtgcttt ttaaagttct tgtaactgtt ttcccccatc 44341 ccccaRtcca aagtccaatt ttatgtactg cttttaggtg tcacatttct gtagtcttct 44401 ttaatctaga aagattcaac aggctttgtc tttttattct tgacatcctt gaagtatata 44461 ggcaaagtat tttatagaaa gatagtcatt tgggatttat gtatttcWtg tttaggttca 44521 gattatgcat tgttggtgtg gataccacag aagtgttgaa gtgtcataac aggggccaaa 44581 tgatgtctgt ttttcccttc attggagatc tcaactttgt tcacttggcc aagatgatat 44641 ctttcagatt tttccactga aaggttactt tttaattaaa ttttgaaaat aaaaaYgttt 44701 cttacgggag atacattatt agtgggcatt atcctgtaaa gattagcttc cacttctccc 44761 Ygatttatta atcagtttct tcaactataa atattctatc aatatggacc cacaatccag 44821 ttaaataaca tgtagattaa cYgttattat ttccaacttt attgaggtgt attttacatg 44881 tcatagaatt tacccatttc agKcgtacga tttaataatc ttcaggaact ttgaacagtg 44941 gtgtgaccgt catcataatt cagtttagat cattttcgcc ccccaattgg actccttgtg 45001 cttatgtaca gttaaacctc attctcacct caaatctgga gcaaccacta acctactttc 45061 tctatatata catttgctta ttctggatat tttataaaat tgaattttac aatccatggg 45121 gtttttagac tggcttcttt aacttagcat aatgtttcca aggttcatac atgttgtagc 45181 atgagccagt tcttcactcc tttgaatttc caaataaaat tctgttgtat ggatatgcca 45241 tgatttgtct attcatttac ccgtaggagg gcattttgct tgtttgcagt ttgSggttat 45301 gagtaatgct gctatgaaca ttcatgtgct aattgaaaat acttgtctat gttggtggtc 45361 ggcattccta gttaccattt cctgtctgtt tttctgcctt tcctggcact ttccaggcat 45421 gatttcacct tcacttgaac tgcttcattc aggactctgc aaattccctg aagtagaagg 45481 aaaaatgacc acattcaaag tgagtagggc ttgcctctct tgctgttaaa attccatctt 45541 actactcata ttgtcacatt tttatctgtc ttgggaagac tcaaggagaa caacaagcat 45601 ttgagggcat tttgtttgcc agatgcttcc tttgcKgctt ttgaattgac tttccgtttg 45661 gttttagttc ttgttttctt acataatttc ttttctgtag tagataaaat agtaggaatg 45721 gataattctt tcacttcact acttctctgc tcacaaatta gctgttttta tcttgtattc 45781 ttcatcatgt acattcataa ctgtattttt attgatgaca tacctctaat tttaaattgt 45841 gctcgtttta tataatatta tataatgttc catttttata ttggtatcat agtcccaatg 45901 aattaatgta ccttcttatt tttaactact tcacactcat agacRtttag gtggtttagg 45961 aattttactg ttttaagcaa cactgctgtg acagctttcc tatatataac cccttccccc 46021 caaaattaat tctgaaaaca agataggata cttttgacat gtattacatt ggctgtttta 46081 tagatggtag attatttcaa cagggcacag cacatgaggc tctgactgct acttcccaca 46141 aatattgttg ttggagtttt cacacaggaa gagctagatt ctgtgtttgc catgccttac 46201 tcataccttg tatgcatgtt atttattggt ctcttcttat tcctttatgg agtaagcagt 46261 tttaccaaag tagtaggtac cttcatttga aatgtccagt gatactggaa gattggccaa 46321 tcacttgtgt tgtgttacta tatttactca gagaagcaga atgcaggttt tagtctcatc 46381 ttcctcttaa actcataaaa tatgagaaat ctgctcagat tcacataggc aaccaaggtc 46441 aggaatgtta gccattctgc tcatttataa tcaaataagt gagaatgata ttaatacaat 46501 gaattcttct tcctaatcat attaccgaaa ccatgggaat atttacaacc tgctcttaac 46561 catcataagg agaataaggg tacttgtgtt cactcggaag agtgggaagt aggtaaaaaa 46621 tttggaagaa agtatgaaat tatgtaatca caattttaaa tgtgtttatt gacattctca 46681 ttttcatttg acattatttt aaataatcag gagaataaag tctatataaa attattttct 46741 ttacaggatt gataataaaa aatatttaat atgaagtatt cattatttaa ataaaaagag 46801 atcatttttg ccctggaata ctttgcagat atgaaatgag atgaaataaa taacaataat 46861 gacagttata ataaaaatag ataatattta ttaaatactt agtctttgcc acacagtttt 46921 cccaatgcta tatatatttc cttctttaat ccttgaaaaa acgataccag gtgaaaatca 46981 ttatacagac aggaaactaa tgcatgtagg tgagcttgtc caaagccaca catctaggaa 47041 gggacaaagc tttgggatgt gctctgaggc atgatggttc catatcatga cattgaatga 47101 tgcaactaat gYatgtagtt tgtgccttga gcatatagag gatgcaatgc atagctgtcc 47161 cacacaggtc accatatact ggttagtttg acagatacta aagaaaagtt aacaacatcc 47221 acagtttgag tatgaaagga agacaatgga agagaggaag acaatggaaa ttatcaaagt 47281 tgggactaaa cttctaaatg ttactggggc aattggcaac aacacactaa aacctaaata 47341 cctgtagccc aggaggcMgc atgtagagtc ctagagggct acttacaagt agactcaatg 47401 actgcacaaa gtaaaagtac aggacttctt ttatatcatt gcacttctga tgaagctcat 47461 gaaaatctgc gtgtgtcatt gtcaggatat aggcagaatg agtgggaaat ctgtatgttg 47521 acctacatcc ctcaagatct aggacaactt accacacctg tcccctttgc ctacttagtg 47581 ccacccttct ttgaataatc attggtcata agatttaggt tttgtatgtt ggatatttta 47641 ggaggcagtg acattcaagg atgtggctgt ggtcttcact gaggaggagc tggggctgct 47701 ggaccctgcc cagaggaagc tgtaccgaga tgtgatgcta gagaacttca ggaacctgct 47761 ctcagtaggt gaggacaggc accctctgta acagaatgtt aggccccagg aatggctttg
47821 tattccaagt ttgagtatgc attgggaacc taaatttcca gtaaattttc cctagatatt
47881 acctacaatg ggttggaatt aagcatgtca ctgtgtgttc acagggaatc aaccattcca
47941 ccaagatact ttccacttct tagggaagga aaagttttgg aagatgaaga caacaagcca
48001 aagagaaggg aattcaggta agaaccaagt aactgtgcat ccttgtacat gactcttcca
48061 tctgttttac ttctgtccat gcctatttcc atcaccctgg tctaaattgc caaactKctt
48121 tcataactta ttgcaactgc cttccttctt taaagttcct ttggSttcat tattcatgtt
48181 catattttat tagtttaata atttatcata caagatttta gactaggaaa tttaccagaa
48241 ttagtaaYgg gttaagtgta tgtatgttat taattattta attcagtctt ttatttctac
48301 caggatttta catatgagat gtgatgtggt tctaaattgt tctcttgaaa accaaagacc
48361 atagtgcact cggaaaacat gaacttaatg tttcctgttt tcattaaagt ttaatgccat
48421 gtcctaagtg tgaaatcttg aaaacattga atagggcttc acttgcccac atatattaat
48481 tctgtgtctt tttaggaggc aagatccaaa ttgagatgga gactgttcca gaagcaggac
48541 cacatgaaga gtggtcctgt cagcaaatat gggaacaaat tgcaagtgac ctaaccaggt
48601 ctcaaaactc cataaggaac agctctcagt tcttcaaaga aggtgatgtc ccctgccaga
48661 ttgaggcaag actatctata agtcacgtgc aacagaaacc ttaccgttgt aatgaatgta
48721 aacagtcctt cagtgatgtt tctgtctttg atcttcatca acaatcacac tcaggagaga
48781 aatctcatac atgtggtgag tgtggaaaaa gcttctgtta cagcccagcc cttcatattc
48841 atcagagagt ccatatggga gaaaaatgct ataagtgtga tgtgtgtggt aaggaattta
48901 atcagagctc acatctgcaa actcatcaga gagtccatac tggagagaaa ccattcaaaY
48961 gtgggcaatg tgggaaaggc ttccatagta gatcagcact taatgttcat tgcaaattgc
49021 acacaggaga gaaaccttat aattgtgagg aatgtgggaa agccttcatt catgattcac
49081 agcttcaRga acatcagaga atccatactg gggagaagcc attcaaatgt gatatatgtg
49141 gtaagagctt ccgtgttaga tcaagactta ataggcattc catggttcac acaggagaaa
49201 aagcattcag atgtgataca tgtggcaaga actttcgtca gagatcagca cttaatagtc
49261 attccatggt ccacatagaa gagaagccat acaaatgtga gcaatgtgga aaaggcttca
49321 tttgtaggcg agatttttgt aagcatcaga tggtccacac aggagagaaa ccatataatt
49381 gtaaagaatg tgggaagacc ttcagatggt cctcatgtct tttgaaccat cagcaagtcc
49441 acagtggaca aaaatccttc aaatgtgaag aatgtgggaa gggattttat acaaattcac
49501 gacgatcttc ccatcagaga tcccacaatg gagaaaagcc atataactgt gaggagtgtg
49561 gtaaggacta taaaaggagg ttggatcttg agtttcacca gagggtccac acgggtgaga
49621 gaccctataa ttgtaaggaa tgtggcaaga gctttggctg ggcctcctgt cttttgaaac
49681 atcagagact ccacagtggg gaaaaacctt tcaaatgtga agagtgtgga aagagattta
49741 ctcagagtWc acaacttcat tcccatcaga catgccatac tggagaaaag ctatacaaat
49801 gtgagcagtg tgagaagggg tacaacagta aatttaatct tgacatgcac cagagggtcc
49861 acRggggaga gcgaccctat aattgtaagg aatgtggaaa gagctttggc tgggcttcat
49921 gtcttttgaa acatcagaga ctccacagtg gagaaaagcc attgaaatct ggagtgtggg
49981 aagagatcta ctcagaattc acagcttcat ttacatcagt aagtctatgt gggagaaaag
50041 ccatataaat gtgagaagtg tgggaagggc tttggctggg cctcaactca tctgacccat
50101 caattctcca cagcagagaa aaaccattca aatatgagaa ctgtgggaag agctttgtac
50161 atagatcata tctttttttt ttttttttga gacagagtct cactctttca cccaggcctg
50221 actgcagtgg cgctatctca gctcactgca agctccgcct cccaggttca cgccattctc
50281 ctgcctcagc ctcccgagta gctgggacta caggtgcccg ccaccacacc cagctaattt
50341 tttgtatttt tagtagagac ggggtttcac cgtgttagcc aggatggtct cgatctcctg
50401 acctcgtgat ccgcccgcct tggcctccca aagtgctggg attacaggcg tgagccactg
50461 cgcccggcca tagatcacat cttaaagacc aagaaaaRag tccacagtgg agataatcca
50521 tacaaatgtg aggagcatgg gatgtactac aacaggcact tgacttgata tgcatcagag
50581 ggtccatatg gcagagaaac cgtggaagtt tggggagatt gccatgtgct tcagttatgc
50641 ctcaaagtct tcaacttcat tggactgttc actttcaagg gaaaccttag taaggtgata
50701 tctgtgatag tcttctcaac tacaagctca tctgagagtt cacaatggga gaagcattaa
50761 aaatttgata catgtggtaa gcacttcagt ttgagttcat atctcatagc ttattacaga
50821 atccatgctg atgatacatt tcatacagtc tcaggaggga aaatatttaa gttaaagtca
50881 gagtttcagc catagttcag catacccacg tggttttaga accactgtag cacagaacct
50941 ttgtaatttg gggcttcatt cagaagtttg gcaattatag ttattcaaga gacaggcctt
51001 agaaagaata ggagtttgtt caggcattta caaatctcta atgatggaca tgtcaaaatt
51061 gatgtcggct agaatataag ctgcatttac ctttgctgca gaatcttcac aaccttaaca
51121 ctgattggca ctttgtatgt gtgtttggta catgtaagag atgtagaaag agctttggct
51181 gggcttcata tcttttgaaa tatcagagat gtgatattaa aggatcaaaa ggagaaaaca
51241 tatttcaaaa ttgtatctaa aagaggaaac ctgggataga ccaagatggc caattagaag
51301 cagctatggt tcatggtact catacagagg aatgaaaatg acagataaat tcagoacctt
51361 caaYtgaaat atctaggttc tctcattggg actgactagg caatcaattc aacccacaga
51421 aaatgaagaa aagtagggtg gagttatggc ccacttggtg tggcatggag ccaaaggaaa
51481 cctcaccccc acccaaggga agcagtgagt gattgtgcga ccctgcgcag gaaaccatgc
51541 tactcaagga tctttgcaac ccacgggtca ggagatctcc ttgtgagccc atgccaccag
51601 ggccttgggt ttgatacaca gagctgtgtg gagttttggc agagcggcct ctcaggcaca
51661 cacagagacc caggaggttt acatgctcta gccccagaat tcctggcaag gtggcagatt 51721 catccttata ttcccctagg aagggggctg aatccaggga gccaggcagc aacattctgt 51781 gggccccact tccatggcac cttacaaaac ccactggctt ggaatttcaa ccaaccaaca 51841 gcaacaggct tcagcctgaa ataggaggga gctcctgtgg gggaaggctg gccaccattt 51901 ctgtggttca gtctactcag cattccagcc tgccagctct ggagagacca ggttgtctgg 51961 acaaggagag gatccccctg cccaacacag aacacctgct ttgccagatc atgcccagac 52021 tgctgctttt tttttttttt tttttttttt ttttttttga gatggagtct cactctgttg 52081 ctcagactgg agtgcagtgg catgctcttg gttcatggca acctccacct cttgggttca 52141 agcaattctc ctgcctcagc ctcccaagta gctgggatta caggcaggta ccaccacacc 52201 tggctaattt ttgtattttt catagagaca gggtttcacc gtgttggcca ggctggtctt 52261 gaactcctga ccgcaggtga tccgcctgcc tcagccttcc aaagtgctgg gattacaggc 52321 atgagccact gcacctggcc cagactgctt ctttaagcag gaccctgatc cattcctccc 52381 cactgtgtgg ggtctccctg tggggggtct tcagtcactc cagccaggct tatactgaca 52441 gaaatctaat ctctacctca gatggagctc ccaggggagt ggcgaccact gtctctatgg 52501 ttcggttgac aaagctgttc cagcctgctg gctttggaga gtctgggcaa tctgggtgag 52561 gaaggcftcct cccccaccgc agcacacctg ctgtaccaaa agcagttgga ctgcttctgt ,52621 aagtaggtcc ctgatcctgt tcctcctgac tcagtgagac ctcccaacag gagtctctag 52681 acacctccta caggagtctt caggccagaa actggtcagt atccccctgg gacagagttt 52741 ccaaaagaag gagaaggctg ccatctttgc tgttttgcag ctttcactgg tgacacctcc 52801 aggtacggga aaaacggagg caactagggt ctcgagcaga ccccagcaaa ctgtggcagc 52861 cctacagttg ctaacagtca gtggcttgac tgttaaaaac aggcagaaaa caacaagaac 52921 atcaacaaaa agaccccaca aaaaccccat tcaagggtca gcaacctcaa gattgaaggt 52981 aggtaagccc acagatatga taaaaagtca acacaaaaaa atgctgaaaa ctcaaaaagc 53041 cagagcgcct cttctccaaa tgactacagc acctctctag caagggtaca gaactgggct 53101 gaggctgaga tggctgaact gacagaagta ggcctcagaa agtgggtaat gaactttgct 53161 gagctaaagg agcatggcat aacccaatgc aaagaagcta agaatcatga taaaacaata 53221 caggagctga tgaccagaat agccagttta gagaggaaca taactgactt gatggagctg 53281 aaaaacacaa cacaagaact taacaatgca atcacaagta tcaattgtag aatagaccaa 53341 gaggaggaaa gagactcaga gcttgaagac tatctttctg aaataagaca ggcagagaag 53401 aatagagaaa gaatgaagag taatgaacaa aacctctgaa aaaaaatatg gagttatgta 53461 aaaagactga acctatgact gattgcggaa cctgaaacag atggggagaa tggaaacaag 53521 ttggaaacat actttaggat gttatccagg ataactttcc aaacctagca tgacaggcta 53581 atattcaaat tcaggaaatg cagagaaccc cggtaagata ctccatgaga agatcaaccc 53641 caagacacat aatcatcaga ttctccaagg tcaaaattaa aaaatgtaaa gggcagtcag 53701 agagaatgaa aggtcaggtc ccctacaaag ggaagcccat cagactaata gcagacatct 53761 cagtggaaac cctacaagco agaagatatt gggggtcaat attcagcatt cttaaagaat 53821 tctttgccag aatttagtat ctggccaaag taagctttat gtgcaaagaa ataagatcct 53881 tttcagacaa gcaaatgctg agagaattca tcaccaccag gccttccttg caagagttcc 53941 tgaaggaagc actaaatatg gaaagaaaaa accattacca gccactacaa aaacacactg 54001 aagtacatag accaatgaca ctataaagca accacataaa caagtctgca aaataaccag 54061 ctagcatcat gatggcagga tcaaattcac acataacaat actaatatca aatgtaaata 54121 ggctaaatgc cccaattgaa agacagagaa gtgcaagctg gataaagagc caagaccgat 54181 tgatacgccg tgtttaagag acccatcaca cgtgcaaaga cacacatagg ctcaaaataa 54241 agggatggag gaaaatctac caagaaaatg gaaatcagaa aaaagcagga gtcacaatcc 54301 tagtttttga caaaacagac tttaaaccaa caaagataaa aaaaaaaaaa aagacaaagc 54361 aaggcattac ataacagcaa agggttcaac aagaagagct aactattcta aacatatata 54421 cgcacccaat agcagaggac ccagattcat aaagtaagtt cttagagacc tacaaagaga 54481 cttagactcc cacacagtaa tagtgggaga ctttaacact ccattgacaa tattagatca 54541 ttgagataaa aaattaacaa agatgttgag gaccttaact cagctctgga ttaagcagac 54601 ctgatagata tctgcagaac tctccactcc aaaacaacag aatatacatt tttctcatca 54661 tcacatggct cttaaaaatt gatcacataa tcggaagtaa acactcctca gcaaatgcaa 54721 aagaactgaa atcataacag tctctcagac catagcacaa tcaaattgaa ctcaagatta 54781 agaaattcac ccaaaaaatt aaaacaaaaa tgaaattaac tcagaaccac acaactatgt 54841 ggaaattgaa taacctgctt gtgaatgact octgggtaaa tactgaaatt aaggcataga 54901 tcaagaaatt atttgaaact aataagaata aagacacaat gtaccagaat ctctgggatg 54961 cagctaaaga agtgttaaga cagaaattaa tagcactaaa tgcccacatc aaaaagctag 55021 aaagatctca agttaacaac atcacaacta aaatagagaa ccaaaaacaa accccaaagc 55081 tagcagaata caggaaataa gatcagagca gaactcaagg agacagagac acaaaaaatc 55141 tttcaaaaat taacaaatcc aggagctggt tttttgaaaa aaattaatag actgctaact 55201 agactaataa agaagaaaag agagaagaat ccaacacaat cagaaatgat aaaggggata 55261 tcaccactga ccccacagaa atacaagcaa ccatcagaga atattataaa cacctctctg 55321 cacataaact agaaaatcta gaaaaagaga ataaatttct ggaaacatac accctcctaa 55381 ggctgaacct ggaagaaatt gaatccttga atagaccaat aacgacttct gaaattgagg 55441 cagtaatagt ctaccaaaaa aaaaaaaaaa aagcccagga ccagacaaat ttacagctga 55501 attctaccag aggtacaaaa aagagctggt accatttcta ctgaaactat tccaaacaat 55561 tgaaaagaag ggattcctcc ttaactcttt ttatgaggcc agcatcatcc tgataccaaa 55621 atctggcaga gataaaacga aaaaagaaaa cttcaggcca gtatccctga tgactatcaa 55681 tgcaaaaatc ctcaacaaaa tactggcaaa ctgaatccag cagcacatca aaaagcttat 55741 ctaccacaat caagttggct tcatccccag gatgcaacgt tggttcaaca tatgcaaatc 55801 aataaatgtg atcacataat gagaaccaaa gacaaaaatc acatgattct ctcaatagat 55861 gcagaaaagg cctttgataa aattcaacat cccttcatgc taaaagctct ctgtaaacta 55921 ggtactgaag gaacatacct caaaataata agagccatag atttcaaacc cacagccaat 55981 atcatactga gtgggcaaaa gttgtaatca ttccccttga aaactggcac aagacaagga 56041 ccagcccccc gccctttccc cactcctgtt caacacagta ttggaagttc tggccagtca 56101 agtcagacaa gtgaaagaaa taaagcatat tcaaatagag aggaagtcac agtatctttg 56161 tttgcagatg acatgatcct atatctagaa aaccccatca tctcagccca aaagcttttt 56221 aagctgataa gcaacttcag caatgtctca ggatacaaaa tcaatgtgca aaaccgctag 56281 cattcctata aaccaacgac aggcaagcag agagccgaat catgaatgaa ctcccattca 56341 caattgctac agaaagaata agttatctag gaatacagct aacaagggaa gcgaaggacc 56401 ccttcaagga gaactacaaa ccactgctca aagaaatcag agaggacaca aatggaaaaa 56461 catttcatgc tcatggatag gaagaatcaa tattgtgaaa tggccatact gcccaaagta 56521 atttatagat tcaatgctat tcccattaaa ctaccgttga cattcttcac agaattagaa 56581 aaaaaaacct attttaaaat tcatttggaa ccaaagaata gcccaaatag ccaagacaat 56641 tctaagcaaa aaagaacaaa gctggaggca ttatgcaacc caacttcaaa ctatactcca 56701 aggctacagt aacgaaaaca gcatagtact ggaacaaaaa cagacacata gacaaatgga 56761 acagaataga gaactcagaa ataagaccac acacctacaa ccatctgata ttcaacaaac 56821 ctgacaaaaa caagcagtgg ggaaatgatt ccctatataa taaacggtgc tgggagaact 56881 ggctagccat gcacagaaaa ttgaaactgg accccttcct tacaaacatt atacaaaaat 56941 taagatggat •taaaaactta tgaaacccaa aactataaaa accccagaag aaaatctagg 57001 tgataccatc caggacatag ggacaggcaa agatttcacg atgaaaacac caaaagcaat 57061 tgcaacaaaa gcaaaatttg acaaatggga tctaattaaa ctaaagagct tctgcacacc 57121 aaaagaaact attatcaggg tgaacagaca acctacagaa tgggagaaaa tctttgcaat 57181 ctgtccatct gacaaaggtc taatatccag agcctacaag gaacttaaac aaatttacga 57241 gaaaaaaaac cattaaaaag tgggcaaaag acatcaacag gcacttctca aaagaagacg 57301 tacatgcagc tgacaaacat gaaaaaaaag ctcaacatca ctatcattag ataaatgcaa 57361 atcaaaacca caatgagata ctgtctcaca tcagtcacaa tggcaattat aaaaaagtaa 57421 aaaagaagcc atttgcagca gctcaaagtg ctgtaatccc aaacctttgg gaggctgagg 57481 tgggtggatc acttgaggtc aggagttgga gaccagcccg gccaacatgg cgaaacgaac 57541 ccctgtctct actaaaaata caaaaaatta gctggatgtg gtggtgcatg cctataatcc 57601 ccagctactt gggaggctga ggcatgagaa ttggttgaac cggaggcaga ggttgcagtg 57661 agtcaaggtc atgccactgc acttcagcct gggcaacagt gaaactgtgt ctcaaaaaaa 57721 aaaaaagtca aaaagcaaca gttgttggtg aagttgggga gaaaaaggaa tgcttttaca 57781 ttgttagtgg gagtataaat tagttgaacc attgtggaag acagtgtggc aattcctcaa 57841 agatttagag gcagaaatac catttcacct agcaatccca ttactgtaat atataccccc 57901 aaattataaa tcattctatt agaaagatac atgcacttgt atgctcactg cagcactatt 57961 caaaataaca aagacatgga ctcagcccaa atgcccatca atgatagact ggataaagaa 58021 aatgtcatac atatagacca tggaatacta tgcaaacata aaaagaaatt agatcatcca 58081 ggacatggat ggagctagaa gccgttatcc tcagcaaact aacacaggaa cagaaaacca 58141 aacactgcat gttctcactt ataagtggga gctgaatgat gagaacatat ggacacatgg 58201 tgggaaacaa cacacactgg ggcctcttgg aggggtggag ggagggagag catcaggcag 58261 aatagctaat ggatgctgtg cttaatacct aggtgataga ttgatctgtg cagcaaacca 58321 ccatggctca catttaccta tgtaacaaac ctgcacatcc tgcacatgta cccctgaact 58381 taaaRtaaaa gttgaagaaa aaactaaatc atagtattaa aaaggagaaa tcttgcaaga 58441 aataatttat ggaaacatga gttgaaatgc agggtacact cagcactgca atgtagacct 58501 ttattttgat tattatctta ttccacttgg ctcctggctt ctcctcatcc tctgttctca 58561 agtcggattc ttattatttg ggactctgtc tagtgttccc ttgattgtat atagtataaa 58621 tttaacaatc atttgtcact tttctcacaa tatgaggtga aaagattttg taataacaga 58681 ccactgcata ttgtacaatt aagttttttc aacaatttta aggcaggatt cacagtaaac 58741 acttttttct tttaaaaaat tatttttaag gaatagagac agtctcacca tgttgcttgg 58801 ctggccttga acttctaggc tcaggcaatc ctcctacctc agcctcccaa agtgctKgaa 58861 ttataggcat gagccaccac acccagccca cagtaacatt tctaatgtgg cagatgtagt 58921 tacattgaca aaaatttttc tggtaaaggc caggtgtggt ggctcactcc tgtaatccca 58981 gcactttggg aggctgaggc aggtggatca cttgaggcca tgggttcgag atcagcctgg 59041 tcaacatggc aaaacGCcgt ctctactaaa aatacaaaaa ttagctggtg tggtggtgtg 59101 tgcctgtaat cccagctacc tgagaggctg aggcafgaga atcgcttgag cctgggaggc 59161 ggaggttgca gtgagctgag attgtgccac tgtactccag cctgggcaac agagcgagac 59221 ttcatctaaa aaaaaattcc taataagttt tgaattgatc atgatttgtg attctgcgtt 59281 tctgtgcagg ctttaatatg attgccttct aattgctgta gttatttctt ttatcagact 59341 tctcaggtag aagctttgcc aaatttgaag gaaaattttt ggcaacctca gtatttcttc 59401 ccatctctat gaactaccaa gaacatttgt tggagtttgg tgatttcatc atacatattc 59461 attattggag caagagtaac ctttacatac tcttctttat tgttggccat aagaatacgt 59521 tttttggaaa gtggtatatg gcagtatgta taaggtacaa ctgttcaacc tggaaatcat 59581 aggatagaaa attacaacat taaaactctg aaaattagta gattacatgg atgatgtcac 59641 ttaattttaa aatggaaaga acaagtgttc aaccacagga atttgtatga agtgcattta 59701 ttaatataaa aacacaaagc cagtaaaatg atgacataga tgtacaataa aaagagaaga 59761 gcataatgta tgccttaggg aaatcctatt gtagccttca taacaaacta agtgtctttg 59821 tgtttctggg tttgtttatt tggttatttt tagtgtgtat gtacatcatt tgtgRatgaa 59881 tattctgaca ttttccctgg caatgagtat ttgctggaat ttttcatttt ataattttat 59941 gcattttaaa tttctcattg aacaccaatt ttaccaaagc agttatgtga tgaaaaattt 60001 gaaaaattat taatattaat cacttaccta taaggactct gaataagatt gctctacatt 60061 gtcatatgac agttcaaaat taaggaatag ggatgtggaa gtcatatttc aaaataaaaa 60121 aatgtatctg gtcacctgga tatacagatt gatgagtgtg gtcaactgcc ttcataacta 60181 atgtgtgtga ttctgcttta ggcataagtc aggaagcaag caaatcctag tgacagaaat 60241 aatgtgggta tatatagttg tggaggttaa aaaaacagat tctttgaaga acaaatgcgc 60301 atgatcaggg aacttaacat gacagcagca gtctcttagt gtgtggaacc agctcggaca 60361 aagagctgat ttgggtgcta attcatagtt atacaccatg gtgtaactta actgtattag 60421 gttaaaaggc attcttcaaa gtgtctagtt gagatagtaa gcttttcata attaattttc 60481 taaataatgt attttcatga tgctatattc atgctgtgcc agtttggata gtagattgga 60541 ggtccctaag accacctctg ggttctgtga tttactaaaa taactgagac tatagttgta 60601 cttataacaa tattacagtg acaagataca aaacagaatc tgtaaaggga aatggctcat 60661 gggcagttgt ctagaggaaa ccagaagcaa gcttaccaga gtcctctctg catggtgtta 60721 cataagactc aactcctcta gcattgaatt gtgatactac ttgtgaaatg tctacaatgg 60781 aagctcatta gcaaccagca gcacaggttc ttattgagat tgattatgta ggtatgtgtc 60841 tagtacatat caagattccc aactctaaga agaagagcaa gtgttccaaa taagctgcat 60901 tgtagagttt aggcgcagtg accactccta cccattatgg aaaggtggta actctctcaa 60961 accccatgtt ccttgactcc agccaaaggc cagtgttgca aacagctctt tttaaagtta 61021 gcagtctgac ttatgcctgc tatgttaact ttttgtgcat gtggtatcag aaaagtaggg 61081 cctgcagtct tgtggggttt tcttaaacta tcaccagata atagttcatc tctaagataa 61141 ttatttgtaa aagaaaattc atcagattac atttttaaat atttttataa taaaaaatac 61201 cataggtagg ccttccatct accctcgtgt gtgYctgggt cctcaaattg gtcacaggat 61261 gatattaaca gctgatttgt ttttattgtt catatttatg tctcacagac tgcatgagta 61321 tttgactccc aataaaactg aatattgaat acctagcttc atatgtagta cctgagcttt 61381 tgtgtggcca gtatttcttg ccacttctca cgatggactg ggtctctttt atgtatatga 61441 ccctggcaag ttcactatgt taactttgaa attgggtaca ttattataat tgggaattat 61501 tgcagagtct caatctctgt gtttgaaaaa tgagggaagc ttcccaagtt attattctct 61561 gaccatatca gtaggatatc catttaaatg tttgagctaa atgccacaac atttgtgtgt 61621 gtgtgttttt tttttttttt tttttttttg atacactatg acaataactt cctggataga 61681 tttgttgttg tttttaattt ttatagtaaa aatacactca ttttacttgc aagaataatg 61741 tctagacatt ggaaacaatt actttggtac agaaaagtct aaaaaagaaa gtggaagata 61801 ggaagtatga atattttggt aaggatggtt cagcatatct atgcatatag gtaacaattg 61861 tatagaattt tacaaaacta ggaacacact aagcatactt gctcctaaca ggattgtcct 61921 aatatgacac taatgttaaa ggctgtataa ataagttgac ctccaacctc atttctaatg 61981 cctgctgtat cctggtgtac tgaatattaa tttttcctta aacagttttc tgatgatgag 62041 gatctgtgtg gtatcaattt taattacaac atgcctagac tacagtccct agttatttaa 62101 tcaaacagta atacatatat atatatatat atatatggaa ggcattttgt agatatttag 62161 cccataatca gttggctttg agtagggata ttatgtgaga taatgtcagt gggcctgatg 62221 aatcacttga aattatttaa aagtagagct gaagcttctt tgaaaaaaga aattgcacct 62281 tttagtagca tcttcctctc gtgggttagt gttccatctg agccatcctg gggtatagcc 62341 atatgttttt tgcagttgct tagccagtcc cacagtggca taagaaagtt ccttgcaata 62401 aatcatttgt atatgtcata ttgattttgt tactccgttt caacgctgac caaaagatat 62461 tttgttccag aagtggttct agaggaagag aatcttaaga atgagttttc taaatcaatt 62521 ctgtgttttc tcaaattgga tttctcatct tttttagttt aaaggcacta ttgactttat 62581 ttccagtgga aaatagggca ttggttgtta aagccacgac atggtgataa atatatgcca 62641 aatatattgg ataatcccaa gcatatacct atagaaggca tgattctaga tgacYatgaa 62701 tttgttagtt tcggacataa tagtcaaacg aaggactata gtggtattta ctagttgctc 62761 tgatttgtgc ttcagaaagt gggaaagaaa aagatgagga caaggcatca aattccagct 62821 caatcagaat aacctgaaag cgtcagtcat tatgcatggc ttcttaacct tgtgttgttg 62881 atcttggcat tcatagtaaa taacttgaca ggaaatctat tacactgtta ctttatttgt 62941 ataggcagga ggattctctt gcaagtaaac agaaatccaa ctggaattac ctaacagaat 63001 cacatctccc aaatcaattc taagacaagt ccagtttaca aatccacaat tgcttcaata 63061 aacgggaggt cagttctgta ttaggatgga cSctggtacg ttgcagagtg cttcacaagg 63121 ccctacagcc attaacccgt gtgactatgt actcaaaagg agaaatattc aaacatcttg 63181 gcaacgacta actcctatta tcaaatggca cgtttatatt ttgatgattg ggcctggtga 63241 gaaggaagca ggaaggaatc taggcatatt tgtaagatac atacatacca gagggaagaa 63301 ataaatctta taataattca tgggcctttc accttggtga aatttccaca agttcactga 63361 tgtaggccta ttgagctatc ccttctgggt gtagggcaag tagaatatag cccttcctac 63421 gatcaaaaaa tagcgtcaca acgcctagtg gaccactggg gctattgtat gctacttatt 63481 cctcattgag gcgtgctact ttgcctattt tgtgtgacct gaaaacattt tgRctcatcc 63541 aagataagtc tgtgcaatgt gtcccagctg ctatgtaagt ttctctgcca tcaggaccct 63601 atgacccaga aaagccaaaa gtgattaaaa tgttaatgaa agatagtgat ggttgagcct 63661 ttggctggcc ctagttagta aatcagcact ttacaaggct gaggtgggag aatcgcttga 63721 gcccaggact tcaagacaag ccttggcaac atagcaagac cttgttctaa aaaaaaaaat 63781 tgaggcgaac agaccaaaaa ctcaacataa aaatgggtaa tagatttgaa caatttacaa 63841 aaagaaaaat aatattgaca caggaaatat gcaaaaatgt tctatgttat ttaaaaaaca 63901 agaaatataa attaaaacta aatttaaata cacttcttac catttgattg gcaaaacttt 63961 aaaaatgttg taataacaca ttttgttagc tagtttcaag gcaagagtat atttatacat 64021 tacttctgca aatggaaatg gtaccaaaaa attaaggcaa cttgacaata tgcaacaaaa 64081 tttctattaa tatcttcact tttcaatcct gctttctggg atgtactctt ctgatgtatc 64141 ttcaacaggg ataatataca aatgtacaag tgtatttata acagcatcat ttgtaataat 64201 aaaatattgg aagtcactga aatgtccata aattgcagca ctgttatata aaccactcaa 64261 tggaatttat gcagttttaa aaataaatta ctccatatga catgatttcc aagatgttat 64321 tatgtgaata tagaagaatt agaagtgtac tatcctaaca tttaagtaaa aaggaaaaat 64381 aagaaacaca tctattctgt taaaaaaaag tggtaatgaa aataataaaa ttagttacat 64441 aaaggtgtta ggtggcaaca ctgtggaatt ggtttagggg taggaaggag gggagtggca 64501 cttctctgag aataatatgt ggtagagcat tgactcatgg gaacatgttg atgactgaca 64561 tacccaataa tataaaatca agaatgagat ttctttgtag ataaactagg aaagtgaaaa 64621 tcatagaata acaaattaac ctaaatgtat ttcaaatcaa taacataaac agtctaaagg 64681 tgacagcaag aagtaattta agcaacttat taaacagtac agtgtttgac tatatatcct 64741 cagtctaaag acaaaaagaa ctataaaaaa tgttgaactc tacttttgca ttgtttttta 64801 caatgatatg aattagtaac tctgacactg catgatgcgt atgcaatggt cttaatgttt 64861 gtggcccacc aaaatccata tattgtaatt tttaccctca atgtgatggt aataggaggt 64921 ggggcctttg gagggtgatt aggtcacggg atgaataccc ttataaaaaa tgccagaagg 64981 agcttgtttg tcacttcaac caaatgaaga cacagcaata tggtgctaac tatgaacaag 65041 aaagtggcca actacctgac acaaaatctg ccagtgcttt gatcttggat cccagtttcc 65101 agatctgtga agaatagatt tctgttgctt aaaagccggc cgggcgtggt cgctcacgcc 65161 tgtaatccca gcactttggg aggccgaggt gggcggatca caaggtcaga tcgagaccat 65221 cctggctaac acggtgaaac ctcttctcta ccaaaaatac aaaaaaatta gccgggcatg 65281 gtggcgagca cctgtagtcc cagctactcg ggaggctgag acagaagaat ggcatgaacc 65341 tgggaggcag agcttgcagt gagccgagat cgcgccactg cactctagcc tgggcgacag 65401 agcaagactc cgtctcaaaa ataaaataaa ataaaataaa ataaaataaa ataaaataaa 65461 ataaaataaa ataaaagcca ggcaggctat gatattttgt gactaaaaaa tattggtact 65521 gagaggtggg cttgctgctg caacaaatac ctaaaaatgt agcagtcttt ggaactatgt 65581 aatggataga gKctagaaga gtttttacat gtatgctagg aaaagcctac attgccatga 65641 atggactgat aaggacaatt ctggttagcg ctcagaaata gagaataaga actgtagaga 65701 aaacatcaat cttgttagag catacctagg tagtcatgat cagaatgttg gtagaaatat 65761 ggacagtaaa ggccattctg aagacctcag tcagaaatgt tgaacatgtt attggaagag 65821 aagagagctt cttgttataa agggacaaag aatttggctg aattatattc aggtgctagt 65881 gttttatgga aggtagaatt tgtgaatgat ataattgttt atctgaatta atttctaatc 65941 aaagtgttga agttgcttga atcatcttga ctacccgtag tgaaaggcac gaagtgagga 66001 atgactgaaa taattattaa ttaaaaggga aacagaacta aaatttttaa aagtttacag 66061 cctatctact ttggaaaaaa aaaaaaacag gaaacctgaa gaatacaagg gtatggttaa 66121 gcaactaact aattcatgaa tttagtatgg attggccatg tcaactgaag ccaggaccta 66181 ttgtccaaga caatggaaga atgaccccaa aggcatttca cagattatgg cagcttctcc 66241 tcccatcaca ggcccagagt gcaaggacct gggggacaga aagggccttt gatgccagat 66301 ataccctaat atattaggca cagtttcctg cctactacga ttgagcgtgt caataaattt 66361 ttatctcttg ggagggcaga acagagcaag gatacagctg taactggttt agaaatagca 66421 gtcactcaRt tttcagagaa gtgttcctca tactgtggtt ggcacagtca ctttatctaa 66481 agaaaaccat gaaaagtatt cttttttagt ttagaaaaat tgatatagaa catgagactc 66541 cattttttta aaataaaaat ataggttgat aggccagaat tcacaggtcc acctgcctga 66661 gccaaaattc tcacgcctcc acagRgcaaa tggactccat ttcccaagat accagcgaaa 66661 cgcctgcggg agtctaattg cccggtcgat gcgcaggaaa tttggctccc gcaggcgttt 66721 cgcgagtaKc ttgggaggcg tgggaatttt ggcccaggca ggaggacctg tgaattctgg 66781 gaagtggagt ccatttgctc tgtggagtcg tgggaacttt ggctcaggca ggcggattcg 66841 ggggattctg ggaagtgtag tccagacgcc tgtggcatca tcgacacttc cggtctccga 66901 gcaggacact gctacttaac aaggtggttt gagccaagta tgtgtcttgc gggtctttgc 66961 gaaagtgaac cagttattag gggcgcctaa cgacctgggg ggtcaactaa gggggtaaga 67021 agccctggag aagacctgag gtttggagat gcgaggggtg ggagggRagt ttcgttgccg 67081 tcgatggagc cttctggcgt tgggggctgt actcaggtcc accggaggag tcgtcggggg 67141 ttcggggggg gcggggaaga actctgtcta gtcttgtgtg cctgtttaga gggttttcca 67201 ggtgtttgac ctogttcagt ccgcctccct ccactcttaa gtgtgcgacc tctttctcgc 67261 gatataacgt ggggtgttac ttaatctccc tgtacctccc attgccgttt tttaaatgag 67321 gatttYagga ccatcctgat agaattgttt taggagttga aagttgtggt ataaaacaaa 67381 gacttaacag ctcctgtcac ttggtgtcct gttaatttcc cgcgaagtgg tcaggtgccc 67441 gggtggggtc agagctccag ctgctccggc ccttctcttg gagcccggca gggctctgga 67501 tgatcccgga cgcctctgac ctctttttag gaaggcgtgg gttgcatcca cgtttcacga 67561 agggagagga gcatttaact tgtatccggg acggcgacgc agagtgtttg acagctttct
67621 ccttccccag cctgactttt ctaaaagagc tgcagggagg gacttggcta ctgaggacaa
67681 agctgtctac acgagtggtc tttggctttt ctctttttaa aaaaaagtta tttattttgt
67741 tagtctgttt ttattattgt tattatttga gaatgaggtt tgctctgtct cccaggctgg
67801 agtgcagcag tggcgcgatc ttggctcact gcaacctccg cctcctgggt tcaagcgatt
67861 ctctgtcctc agctccggag tagctgggat tacaggcatg cgtcaccatg cccggctaat
67921 ttttgtattt ttagtagaga cggggtttca ccatgttggc caggctggtc tcgaacctgt
67981 ggtcctaagt gatccgcctg cctcggcctc ccaaagtgct ggaattacag gtgtgagcca
68041 ccgcgccggg ccggtttttt cttttacaga gatgttcacc tcaaccaaga gtgtatagga
68101 tgtgtccttg ggcatgggtg ttgataataa ttaaacctta attggtaact taattctaag
68161 ggctttatgg aaataaatac atttacgcca caccatggct ctgtggaaag gtattgctct
68221 ttttcttttt tttttttttt tttttttoct gagacggagt ctcgctctgt cgcccaggct
68281 agagtgcagt ggcgcgatct gggctcagtg caataggccg cctcccaggt tcaggcgatt
68341 ctcctgcttc agcctcctaa gtagctggga ttacaggtgc gcaccaccac gcccagctaa
68401 tttttgtatt tttagtaaag atggggtttc accgttttgg tcaggctggt ctgaaactcc
68461 tgacctcgtg atccgaccgc cttggcctcc gaaaatgctg ggattacagg cgtgagccac
68521 cgcgcccggt cgctcttttc cattttaaat atgggagtga gacRcctggt gcttagttcc
68581 cRgtagaatg aggtacagct ggagctggaa cctaggcagt ttgggtcaga gcctgagttg
68641 atcatccctt ggatacattt tcattgaggt agaggaacta gctggaatca ggtggggaaa
68701 gagagttagg acttgattga agggaccttg agagcaaggc tcatagactt ggacttctgt
68761 aggagtcttt taaaaatttg tcaacatcct gtttcacttt ttataaggat tttgtattgt
68821 ggaaccatat cattgtggag cattttaaaa ttagattaat aggaggagaa aacatgtttt
68881 aaattaccca tgaatccaag atgtgaaggt gctaactgtc tgatacaaga gatggaactt
68941 ccagttctcc tccgtttctt tttagtcttt ctctgtgtct attgtatatt tatttataag
69001 catttaaaaa aattgttata atatcttaat gtgttttccc atatttcttt aactatggaa
69061 atttttaaat actgctaata gagaataaac tagtaaactg ccatattatg tcatgctcat
69121 ccaatattat tcccatttgt gttttttttt tttttttttt agacggagtc tcgctctgtc
69181 acctaggctg gagtgcagtg gtgcgatctc ggctcactgt gagctccgcc tcccgggttc
69241 acaccattct cctgccccag cctcctgagt agctgggact acaggtgccg gccaccaYgc
69301 ccggctattt ttttgtatat ttagtagaga cgaggtttca ctgtgttagc caggatggtc
69361 tcgatctcct gaccttgtga tccgcccacc tcggcctccc aaagtgctga gattacaggt
69421 gtgagccacg gcgcccagcc cccattgtta tcatccttat gactatttcc tgatagttga
69481 aaacctttca tttttatatc atttcaaagc tgtagaagag ttgcaaaaat agtataagga
69541 attctcatat actctttacc caaatttgcc gattgtttac atttttcctg tttgctttgt
69601 tggtggccat cagccaaata ctaccaggaa catatctgta accagagacg attgtagatt
69661 tctgcagcaa ggaagcccac attccatggt gatcatgggg tgacttggta agaggtgttt
69721 agaaagggca ggttataggg tctcacRtaa catgctagac aatattaaga tatcatatta
69781 aatgatattt attaaatatt agatatctgt ttaaaaaact actaattgtg cttatagtgt
69841 tataattaga tagtgctaga tagcatattc cataacatca tgttatctca tatgtggttc
69901 cataattaaa tttgttcaat tttttcattt atagtcctca caactatctt ctaccctacc
69961 ctttaccctg accccagttt aaagtctaat tatatgcatt gtgtttactc gtcacatttc
70021 tgtcttcttt aatctggaag gatttcacag cctttttctt taatatatgt ggtattttta
70081 aagagtgtag gcaagttatt ttggagaaag acgttcattg ggatttatct gatatatctc
70141 atgtttaggt tcagatcatg cattgttatt gcttctgggc cttatgcatt gttggtgcgg
70201 atactgccaa agtgataagg tttccttcct attgtaacat aacagggcca aacgatgttg
70261 gtttggccct tcattggtga tctcaacttc attcatttgg ccaagatgat gtctttcaga
70321 tttgtccact atcaaattac ctttttaaaa gaaatagtca agtttcttct ggggagatac
70381 attattaatg ggcattcact ctaaaaatga actgccagtt ctcccccatt tatttattag
70441 tttcttcaac tataaatatt ctatcaRtat ggactcagtc ttgttaagtt aaatgacatg
70501 taaactaaca attatttttY ccaattctat tgaggtattt tttacgtatc atagcattca
70561 ccaatttcag gtgtacaatt cagtgatctt caggaatttc acatagtggt tttgccatca
70621 tcatgattca gtttagacca ttttcatccc ctaactggac ttcttgtgcc cacttacagt
70681 taaaactcat tttcaggccg ggcacagtgg ctcatgcctg taatctcagc actttgggag
70741 gccaaggtgg gaggtatagc ttgagctcag gagtttgaga ccagcttgga caacatagca
70801 agaccccatc tcttaaaaaa taaaaactca ttctcacctc aaatctggag caaccactaa
70861 cttatattct ctctatatac cattatctat tctggatggt tcatataaat gggatcgtat
70921 aatccatggg ttttgtgatt ggcttctttc atttagcatg atgtttccaa gctttggaaa
70981 catcctttaa atttccaaat aagattccat ggtatggata 'caccacYatt tgcctatcca
71041 tttacccaga ggaaggcatc ttggttgtgt gcagtttggg tttattatga ataatgctgc
71101 tgtgatcRtt catgggctaa ttcacaacac acatctctct ttttctgcag actgtggcac
71161 gtttcaggca ggattcctcc ttcattcaaa ctgcatcacc caggagtctg caaattcccc
71221 aaagtaggag gaaaaatgac cacattcaag gtgagggggg cgtgcctctc tcgctgttaa
71281 aattccatgt cactactcat attttcatgt gtttttctct gtcatgagaa gactcaaggg
71341 gaaaacaggc atttggggac ctgtttgtgc caggtgcttc ctttatctct tttgtgttga
71401 ctgcatttgg tttttggttt tgctttgttt tgttttgtta ccattattta ttttatggag
71461 tatgaaaatt agtagaaatg aacagtgctc ccacttcctt acctcccctc tctcaaatta 71521 tctgttttta tcttctctgc tgttYgtcat gtacatttgt aactgtattt gtattgatga
71581 tatacatcta attgtaaact gtgctctttt tatatgttat ataatcttcc atttttatgg
71641 ttacatcata ttcctattac attgatgtat ctttttattt aKaactacct cacgctcata
71701 gacatatttt aagcaacact gcagtgacag ctttcctata cataaccttt tcccacaaaa
71761 ttaattttga aaaccatatg ccattttagc tcaattttga aagggtactt ttgatatgta
71821 qcacattggc tgttttgcag atagaatatt ttaactaggc acagaacaaa aggatgtaac
71881 tgctgcacca cacaaatatt gttggagttt tcacactgga agagctagat tctgtgttct
71941 ccttgtctca gtcatacttt gcatgtgctt tatttactgg tctcttctta ttcatttatg
72001 tagtaagcag tttaaccaaa gcagtagaaa gccttcattt aaaatgtcca gtgatactaa
72061 gttttgccaa tcacttgtgt tctgttRcta catttactcc tcagcgcagc ggaatgaggt
72121 tcctggtctc ttcttcttct tgaacaggtg aaatatgaga aaccttctca ggtgtacaca
72181 ggcaaccaaa ggtcaggagt gttagccatt cggctcaggt gaaaaagaga aacaaggaga
72241 gagacagaat tctccattga gcaacctgtg ccctgtgatg tggatgtctg gagtgccctg
72301 tggagtgatg gtgtccctag tggtgctgct cctccctgtc cctacttggt gtgacttctt
72361 gtcacatgtY actatgacat ggcatctact ccaggcactg aagtatactg attaccatag
72421 ttgttataga aaatctttaa atcccttttg gcaaaagatg cctcacacaa tgttaccaga
72481 agaaataatg acctggggca gaaatgttWg ccacgtggat acctaaagaa aataaaattc
72541 aaaatcaaaa ccttgagcac agaataaagg aaaaactcca aacagatagg aacRaaggac
72601 actaaaaggc caatgtaaaa ggagaagtag agatggccaa taaccttgaa aagtgatggt
72661 tttcatttgt aatcaaataa gtgagaataa tagaaYgatt tgttccccaa atcagattac
72721 caagactgtg ggaatactta taacctgctc ttggYcatga taaagagaag caaagtactt
72781 gtattctgtt caaagattat ggaggtaggt aaaaaactgg gaagaaagca tgcagttata
72841 tgatcacagt tctaaatgtg cttatttttt gatgttttaa ttttcatttg aaattattat
72901 tttaaacaat gagagagtaa gatgttctta catcttgcta catcattact taatgatgct
72961 ctttacaggW ttgataatgg aaaatatgta aaattaaata ttctttactg tagctaaaag
73021 taggtcatca gatctctgga atactatgca gccatgaaat agataatgct agtaacaaca
73081 gtaaaaaWga ttccagctaa tgttcattaa acagtggcac agRtgtttcc aaatgctatg
73141 tatttccttg tttaatcctt taaaatactg tgccaaataa ggactattat ttagagagga
73201 aagtaaatcc tgtaggcaaa catgtctaag gccRcacagt taggaagggg cagaccctta
73261 ggatgtacaR tgatgcattc tggcttctga tcaggacact gaaggctgca attaacattt
73321 gtggtttgta ccttgagagt agaggacaca ctacataact gtcccactca gctgtccaca
73381 ctggtactgt ggagtaggtt cactgtgcac tgattaccaa cttgtgtgag tcctgtgaga
73441 cagaacacga tctcactcaa cagattacgt gaagcagatt tgttatctac aaattggcag
73501 caagggagca caggagtgta ggatacactg tgaggtggcc atccaaggct gaagaaaacg
73561 tcctggggtg ggtagaatct tgaccccabt tccaccagag cagaggtacc ccagaaggca
73621 gcccacccga ggttatatac ctccggggca atacgactta ctgggcactt tctccctaac
73681 tcaaRacatt atcttcccta ggaaggacag gaacattgca ttgctagcat gttctatgtt
73741 tattcttcaa aactatacac aagaaagaat gattcaaggc caccctgaaa attaccYaca
73801 gttacctaaa acttgttagt ttagcaaata ctaaagaaaa gcaatagcat ccagRgtttg
73861 agtaYgagag aggaggaccc aacaaagttg ggactgaatt gtttaatgtt gctggggcag
73921 ttggcaagat gtgtcgaacc tatatatggg tagctcagga tgcagcatgt agagtcctag
73981 agggttacat acaagtagac tcaatgagtg cacagaataa atgtatgaga cttcatcatt
74041 gcacttctgg tggagttcag gaaaatcagt atgtgtcatt gtcaggatat agRcagaatg
74101 agtgggaaat ctctaagttg acctacatcc ctcaataYtg agaacaactt tccactcatc
74161 cccttcccct gctcaatgcc gcttctcccc cagtagtcct tggtcataag attgaagtta
74221 tgtcttcttg atgttgtagg aggcagtgac cttcaaggat gtggctgtgg tcttcactga
74281 ggaggagctg gggctgctgg accctgccca gaggaagctg taccgagatg tgatgctgga
74341 gaacttcagg aacctgctct cagtgggtga gcacgggcat cttttgtgtc tgaacatcag
74401 gcctcaggag tggttttgta ccttagagtg ttcaagtttg agtgtgcatt gggaacctaa
74461 atagccagta aattttgcct aaatattacc cagaatgtgt tgggattcag cacgtgacct
74521 tacctattca cagggcatca accgttccac caagatactt gccacttcct aagggaagaa
74581 aagttttgga tgatggggac agcaacccaR agagaaggga attcaggtaa gaactaagca
74641 tctgtgtgtc cttgtacctg actctcccac ctgttgtgct tctgYccatg cccatttgca
74701 ttgctctgat gtaaatgacc acatctcctt cctggattat tacaactgcc ttccagttgg
74761 tggccctgtt cccaccctaa tatctgttct catggcagcc aaagcgatcc ttaggaaatg
74821 gaagtcagat tgtcattcct ctcctcaaaa gcttttataa cttcttattt ttattgatgt
74881 tacaacctct ttaagtgcat cttagagacc tatgtggtct gctcttcatc ccattctgcc
74941 tctctgacta cgtgtgtcct ttccatcttt ctccatcatt t'ccagccaca tttcttccct
75001 gctgtttctg tcctgccaag gacactgtat tgcctgttgt ggtaaccaat gaccacaaac
75061 ttagtggact aaacaaaaat atttattatc tctctgttct ttaggcctga agtttgatac
75121 atttcactgg gcaaaaatca aagcgtccct agggctttgt ttctgcctga atgttcttgg
75181 agggaaaact tcactttggt ttaccagtgt ctaggcactg tccacactcc ctggctcttg
75241 tttcacgtct ccatcttcga aggcagctac tttgcatctc tgaccattct tccataatcc
75301 cattccctct gccactcttc ctaagcctct gccttctacc tttcaggtcc cttgtgattg
75361 cattggtcac tcctgcataa accaggatga tcttcctacc ttctgcttag gtgactagtg
75421 accgttatgg cgtctgccgt tttaatcccc cttgctgtgt aacataatag gaaggctgac 75481 tgtatattgc agaacagttt ttttggcctg ctgatggctg cttatccaat tctgactttc
75541 ttaggttcct tgacatgatt ggtttcaggt gtgtacaatt gacccacgtc ccgtggaaac
75601 ttctaagcat tgtttttatg accacactga gtaagatgcc ttccctgaga cttatcctgg
75661 agctttccat agggagcata tttatatctg ggaagaaatt ctttttgaat gaggatttcc
75721 agctacattt cctttgaaag atttacccat ctgcagccca tttgcctttt ccaaagtcta
75781 tatacacttc actggcccca gcttcctcct tcgtactaga agggggaaga atgaggatga
75841 ggggacacaa gtcatgtagc tgaacattta Stctatttct ggtcctcttt cttcataaat
75901 gtgtctattt ctgcatcttt atgtcataca agttttgttt tcaaaaacat taaaggatat
75961 agattttaca caattttttc ttgattttta tagatttagt gctgtatttt gaactggtga
76021 actttatgac ctgttaggaa ttgggaaact gccctctaaa actagaaaca tcctgaagat
76081 tctcctttct ccttaaagat ttcttattaa tttaatttat ttttgagatg cagtttcgct
76141 cgtcacccag cctggagtgc aatggtgcaa tctcggctca cagcgacctc cgcctcccag
76201 gttcaagtga ttctcctgcc tcaccctcat aagtagctgg aattacaggt gcccaccacc
76261 acacccagct aatttttgta tttttagtag agatggggtt tcaccacatt ggccaggctg
76321 gtctcgaact cctgacctca ggtgatccac ccgcctcggc ctcccaaagt gctgggatta
76381 caaatgtgag ccaccatgcc cagccaagac ctcttattta taagtgacag tgtattaaat
76441 atatctacgt accactgtat accaataggt acacacttac ctataaatcc tttccaagat
76501 tcaacctctg caacttaact ttccattgga agaatttact taattcagga cttggtatta
76561 tcctgacaaa gttttgaatg ttttgcaagc atgagccata gtaagaagca catatttcaa
76621 tataacccgg acaaacattt ttcatgcctg tagatgtgta tgagtgactc taggaggcat
76681 ttcatggaat gaccactttc ctgcttgtca tgatgctgtg ataatttaca ttccagttct
76741< tttttgtaaa tgtttattgc agagtttaac tgtatttcat agctcactaa tgtgtagtct
76801 tcaatataaa aagttataaa gtcctattta tctgcatcat aggctttctt ttcatttcat
76861 gtgattcttc acatttttga ttgtctgctc aggagcatcc ccctttgtgg acccctggaa
76921 ggacagcctt tcagattccc ctcaaaactg tagttgtgtg tctctgtact ccttcagata
76981 accaagttac tStccacatc aatacagaaa agtaagatga agtattttac atatgctgtg
77041 caaaattctt gtgcagggat actttgaatg tgtacataga aacactatga agtgtttgat
77101 gtttaatgtt gtgcagagtt caaccaactR caaaatttac tgagacattt tacagagaac
77161 tgctctcact tcacaccaag tgcaaattca ggaggttttc aaaactgcct ttgcattcaa
77221 taattttcta aactctcaga actcactgaa tacttttatt ctcactgact gatttttaca
77281 gggaaaacat gtatattata cttagagaaa aaccaaataa aacagggtcc agatgagaat
77341 aaccaaatgc ggaccttcta ttgtattctc ctggcagagt aggatgtgtt acattcccag
77401 gattaaggta tgacttagac tcaaagtact gtcagtcaca gaaactcacc taagtgctgg
77461 gatccagagt aattattggg tctccattat ggagccagta ttgattgatg attcctaggt
77521 ggttaatcag tctcctagtc cactaactct ctgtgaccta aagctccacc ttaaatcaca
77581 ttgcttattt ctgacatgga gaggagcatc tcccactccc aatcccccaa aggatttgcc
77641 agttgcaacc ataaattata ttgtttcact atgcagagtg atccagtgat tgccttcaag
77701 ccaaccaaga cactctcctg aggtatgaca cttcaggatc ctagagatta cctctcagat
77761 gctgaggaca aagtcccaac ctctttttgg ttaggttaac tttttttttt tttttgaggc
77821 agagtctcgc tctatctccc aggttggagt gcagtggcac gatctcaatc tcagctcact
77881 gcaacctctg ccttccaggt ttaagcaatt ctcctgcctc agcctcctga gtagctggga
77941 ctacaggcat gtgccaccac acgtggctaa tttttgtatt tttagcagag acggggtttc
78001 aaggccaggc tggtcttgaa ctcctgacct caagtgatct gcccacttca gcctcccaaa
78061 atgctgggat tacaggtgtg agccactgca tccagcactt tttttttttt ttttttttga
78121 gacagggtct cgctctgtca cccagggctc actgcaactt ccacctccca ggctcaagca
78181 attcttccac ctcagcttcc caagtagctg ggactacaga gtgcacgacc acacctggct
78241 aatttatgta tttttagtag ggacagggtt ttgctatatt gcccaggctg gtctcgaact
78301 gctggactcc agtgatcaat cccaaagtgc tgggattaaa ggcttgagcc accgcacctg
78361 gcctagttta acttctttat aaggatatac agcataaaca caaatataca aagtgttgat
78421 taagttattg cttttcctta aagttccttt tgattcatat ttcattatgt acataaattt
78481 ttattttcat aatttatcat gcaagatttt ggactgacta ggaaattcat tacaaatagt
78541 aacaagttaa atgtatatca tatttttaaa ttaagtctat tatttctatc aaggatttca
78601 catgtgagac atgatgtggt tctgaattgg cctcatgcaa accagaaatc agggtgtact
78661 tggaaaacaa actgaacatt ctctgtattc attaaagttt aatgtcatgt cctaagtgtg
78721 aaaccttgag atcactgaat aaaggcttca cctgtacaca tctcttaaat ctgtgtcttt
78781 ataggaggca agatccaaac tgagttggag tctgttccag aagcaggagc acatgaagag
78841 tggtcctgcc agcaaatctg ggaacaaatt gcaaaagact taaccaggtc tcaggactct
78901 atcataaata actctcagtt ctttgaaaat ggtgatgtcc cctcccaggt tgaagcagga
78961 ctacccacaa ttcatacagg acagaaacct tcccagggtg ggaagtgtaa acagtccatc
79021 agtgatgttc ccatctttga tcttcctcag cagttatact cagaagagaa gtcttataca
79081 tgtgatgagt gtggaaaaag catctgttac atctcagctc ttcatgttca tcagagagtc
79141 cacgtgggag agaaactctt tatgtgtgat gtgtgtggca aggaatttag tcaaagctca
79201 catctgcaaa ctcatcagag agtccacact ggagagaaac cattcaaatg tgagcaatgt
79261 gggaaaggtt tcagtcgtag atcagcactt aatgttcatc gtaaattaca cacaggagag
79321 aaaccttaca tttgtgaggc atgtgggaag gccttcattc atgattccca gcttaaggaa
79381 cataagagaa tccatactgg ggagaaacca ttcaaatgtg atatatgtgg taagaccttc 79441 tattttaggt caagacttaa gagccattcc atggttcaca caggagaaaa accatttagg 79501 tgtgatacat gtgataagag ctttcatcag agatcagcac ttaataggca ttgcatggtc 79561 cacacaggag agaaaccgta cagatgtgag cagtgtggaa aaggctttat tggtaggcta 79621 gatttttata agcatcaggt ggtccacaca ggagaaaaac catataattg taaagaatgt 79681 gggaRgagct tcagatggtc ctcatgcctt ttgaaccatc agcgagtcca cagtggagaa 79741 aaaagcttca aatgtgaaga atgtggaaag ggattttata caaattcaca actgtcttcc 79801 catcagagat cccacagtgg tgaaaagcca tataaatgtg aggagtgtgg gaagggctat 79861 gttactaagt ttaatcttga cttgcaccag agggtccaca cgggagagag accttataat 79921 tgtaaggaat gtgggaagaa ctttagccgg gcctcaagta ttttgaatca taagagactc 79981 cactgccaga aaaaaccatt caaatgtgag gactgtggaa agaggcttgt acacaggaca 80041 taccgtaaag accagccRag agactatagt ggggaaaacc catccaaatg tgaggattgt 80101 gggagacgct acaagaggcg cttgaatctg gatatacttt tatcattatt tctaaatgac 80161 acataattgt tcatatttat ggggtacaac gtgctatttt aatgtgtgca tacaatttat 80221 agtgatccaa tcagtgtaat tggtgtatct gttacctcaa acatttacca tttctttgtg 80281 ttggaaaatt caaaatccat ttgagaggag ttggtagaca gcaagggaag ggtccctgga 80341 gacccccagc caaacgggtc agtgtctcat ccccacataa cacaaaaagc agcctgggga 80401 agacaatcaa gctacaggca ctgataaggg aactagcaca ggggattgtg cctggagaca 80461 tgcccatggc tgcacagata gaagaacctc cagcccattc aggtaaaaac tcgcacaaac 80521 ctccggctca ctcagttaag ggaacaagac cagacacaga aatgcctttg tcctttgtat 80581 aatcagcagg ctccgaggaa aaagtttctt cttttgtggg catgaacaca gtgggcttca 80641 gtaggttcag gttggcactt tcttttgtgg aaaaggaaaa gcccagcctc tgtaaatcat 80701 cacttcagct Gctgattggt crcaggccaa gctgaatagc ccttaggaat aatcacttca 80761 actcctgatt ggtcccgggc caagctgaat agcccttatg aatcatcact tcggttcctg 80821 attggtcccg ggccaaggtc ccagaccaag ctgaatcacg ctttctccaa gacagcccgc 80881 aggctaagtg cattccttcc ccttcccagt ccataaaaac cccggacccc agcctcatac 80941 tgggcaactt atttggatcc ccatctccac tgtggagagc ttttttcttt cacttattaa 81001 acttttactc caatttcacc cttgtgtctg tgcttcttaa tcatattgga cgtgagatta 81061 agaactccgg gggataagag agactgttac agagagactg ttacagtgtg gtgcatttgt 81121 gagactacaa cacattttta gcaatttgta aataattgtt gatgatagtc acctttagtg 81181 ctgcagaaca gtagaactta ttcttcccct ctacctcttt tgtatctgtt ggccaacctt 81241 tggccatctc acctacctcc tacacttcct tgcctgtcta catccactgt tctactctct 81301 actacaaaat caactttttt taacttccat atgagtgagg acagtcagta tttctctttc 81361 tttgcttggc ttatattaac aatgtccttg aaaactcatc catgttgcca caaatgataa 81421 aattttgttt ccttgttatg tctaaagagg attccattgt gtatatatgc caccttttct 81481 tcattcatgt attgatagac acttaggtgg attccttatc ttgactattg tgagtagtgc 81541 tacaatgaac ctgggagtgc agatatctcc gYgacatact aatttccttt cctttggata 81601 tacccagtaa tagaattgct ggatcacttg aattttatta tgcttcagaa gctccacacg 81661 ggagagaaac tacggaagta tgaggagggt ggtatgtgct tcattcaggc ctcaagtctt 81721 caacttcaga atgtccacat tggagagaag cctcagtaat gtgtgtaata aagtctttac 81781 tcagttttca tgactacagt ctcacctaag cattcacaaa ggggagaaac attaaaatat 81841 gaggcacata gaaagcagtt ccttttgagt ttgtatcttg taatttatca cagaatccat 81901 gctgatgata catttcatYc agtcctgtag gagaaaaaac atttaagtta aagcctgttt 81961 cagccatagc tcagcatacc ccagtggtcc tgggactgtc acagtggaga acccttgtaa 82021 gttgtgcagt agggcttcaa acaaaagttt gacagttaca gttattcagg agacagacct 82081 taagagttca ttcatgcatt tacaaaacga atcatgaaca tatcaaagtt gattaccact 82141 agaatgtcag tgacatgtgt tttctgctgc atcttcacaa ctttaacact gattatcact 82201 ttgtgttcac tatgtgttaa atgaattaaa aggagcaaac gcgatgtttt taaattgtat 82261 ctaagagagg aaatctacaa ataattcagg gacacctgag ttgaaatgca gagtacacta 82321 aatactgcag ttcacagcat tattttgata atatctgttc acacttggtt cctgtcttcc 82381 cctcctcctc tattccgaag ttgcattctt agtatttgtg gctttgtgta gtgttccatt 82441 ggttgaatac aatataaatt caacaataat ttgtaatttt tctcacacta caggctgaaa 82501 acgttgtaat aaattcaata aaacatctta ataaattcaa aagtttaata ccataaaagt 82561 cttttccaat tataatctag atcacaaaag ctgaaattca atcacatcat gaaaagtgga 82621 aaattcacaa gtatgtggaa actaaacaca ctctttatat atatatgtgt atatatatgt 82681 atgtatatat atgtatatgt atgtgtatat atatgtatgt atatatatgt atatatgtat 82741 gtgtgtgtgt atatatatat atatatatat atatatatat atatatatat acccaccctg 82801 tgtccaagtg ttctcattgt tcaattccca cctatgagtg agaacatgcg gtgtttggtt 82861 ttctgtcctt gtgatagttt gctcagaatg atggtttcca gcttcatcca tgtccataca 82921 aaggacatga acccatcttt tttaatggct gcatagtatt ccatggtgta tatgtgccac 82981 attttcttaa tccagtctat cattggtgga catttgggtt ggttccaagt ctttgctatt 83041 gtgaatagtg ccacaataaa catacgtgtg catgcagcac actcttaaat aatatgtcag 83101 agtacatcag gagtgaaatt aggaaatacc ttagacaact gaaaatgaaa acaatggcca 83161 aaacttttgg aatgaggcaa aagcagtagg aggagggaaa tttatatctg taaatgctta 83221 tattaaagaa gaaagatctg acatccacag cctaatttta ttttcatgaa ctagaaaagg 83281 agaatcttac agaaagtgat taggagggaa ataataaaga ttaaagtatt aataaataaa 83341 ataSagaaga agaatacaaa gaaaatcaac caaaccaaga gttgattatt ctgaaagact 83401 aacaaaattg tgaaaacttt agcaggactg actaaaaaag actcaagtaa ctaaaagtgg
83461 ggacattatt acccattttt acagaggtga gccacagcgc ccagcttcat ttttgtataa
83521 Rgtgtaagga aggggaccag tttcaatctt ctgcatatgg ctagccagtt atcccagcag
83581 cacttattga atagggagtc ctttccctgt tgtttttgtc agtgtcatca atggcatggt
83641 ttggatgtgt ccccacccaa atctcagctt gaattgtatc tcccagaatt cccacatgtg
83701 ggagggacac cacagaaagt cagaattatt aattaaaagg gaaacagaac taagaatttt
83761 aaaaattcac agcctatcta ctttggaaaa aaaaWttaga aaacctgttc tggataaaac
83821 aacggtagag ataagcaact gatttaggag attagtttgg attggtcatg tcaacagaag
83881 ccaggacctg ctgtccatta acactggaag aatgacccca aaggcatcta ggagatcata
83941 gaggctgctc ctcccaacac agacccagag tgcaMggacc tgggaagcag aagggacctt
84001 tgatgccaag tgcccctaac cttttaggct cagttccctg catgctacga ttgagtgtgt
84061 caatacatct tatctttcgg gaaggcagag tagatcaagg atacagctat aactggcata
84121 tgaatagtga tcactcaatt ttcagagagc ggatgtttgg catcaaaagt ttggcacagt
84181 caccttattt ttgtgtctaa acaaaatttt gaaaagtttt tagtttagaa aaataggtaa
84241 acagtagact ccattttttt taaaaagaag aatattggtt tataggccaa tgtatgttta
84301 tagggagaaa aaaagttacc ttcaaaagag caggttttta gatgaattgt ttcctaattt
84361 agctttatac tgttattatt ttttaaccaa tgccagtttt taaattgcta actataataa
84421 agaaattatg tttcaccaag aaataagaag aaacttaaaa aaaaaaatYt ttaattattg
84481 aggtatctgt tgcctaggct ggagggcaRt ggtggcatca tagctcactg cagcctcaat
84541 caaactcctg ggcttcagca atcctcccac ctcagcctcc tgagtacttg gaacttgtag
84601 tcccaagatc aggccaccat gcctggccag ttaaaaaaaa atttttatag agacgaagtc
84661 tccctgtatt tccctgtctg gtctccaatt cctgagctca agggaacgtc ctgccctggc
84721 ctcccaaagg gttggcatta caggtttgag ccactgcgct tggccaggaa gaaaactctt
84781 tataagacRt gctccttcac ctatagaatc ctcccgtttt actgcattta caatctcaat
84841 gtaagattga taccaatgcc tagctcttca tcagtcatta ttgctctgag gtactgagca
84901 attcactaca ttcgctgaaa tttgatgttt ctacatctaa ggagaaatat gaataccatg
84961 aaaagtttat gggaagatag ctattccatg acgacaccgc ttctccacta gtagctgtga
85021 tcttaaatgg gggtggcttt tcctgccaaa ccagtggtcc cacagcttct ggagtagggg
85081 ctggtgtctt cctgctcctc attgcccctg ccaaaccatt ccacctggct catcctttaa
85141 accgttagtt ttaacctccc atYgtcagat tatcctaatc cttgaagatt ttatctcctc
85201 gtttgttctc ttcccaataa cactcctgtc atcgtgggag gcgtttagac ttcaggaatt
85261 cctgtctata cactattttc gctcaaagac tcaggaagag agacggacga ctcacagggt
85321 cacagtccac attccccacc aggcgctcag agatatggaa tacgttcccg gtgggcactg
85381 cggtgtgtgt agtcaaggac caccgcggag tccaacgctt cccgctgaag agtgagatta
85441 tagacatttt cctttggcat tggaggaagY ggagtccaga cacactctga gtcgcgggca
85501 agttggctca ggcgggtgga cgaRtgaatt ctgggaactg tagtccatca acttcatgga
85561 atggcggacc ctttggctca agcagttgaa ccggggaatt ctgggaagtg gagtccagac
85621 gctctgtgga gacgcgagca cttccggtgt gtgagcctgc Sgttgctaca taaccgcgta
85681 gtttgagcca tttctgcgtc tggcgggtcc ttctgaactt gtcaccttcg cttggggtcg
85741 caacgacccg atgatcgatg atccaagcaa gggaaaagaa gccttggcgg agagcggagg
85801 tttggtgggg gcggggaatg gggttttttt cccgtccacg gaagctttct gggatggggg
85861 tgctgtgctc gcatcccggg ggttggaatt ggcggggtcc tctgtgccct gctgtgagcg
85921 tttccaggac tttgacctcg ctcagcctgc ctctctccac cctacctgtg cgaccgcttt
85981 ctcgcagtgt gacgtggagt gttactcaat gtccttatac tttccattgc tgtttttggt
86041 aatggggaca ttagaaccat cctagtagaa tcgtttttgg gaactgaaag agataacgca
86101 aatgagaacc ttaaagcagt ccctgtcact tggtgactgg tggtctcttg ttaacttccc
86161 gaagaggtca ggtccccggg tggagtcata gcttcagctg caggggccct ggtccttctc
86221 cctaatccct gcaggacgct ggatgatccc gggaggcctt ctgacctctt tttaggaaag
86281 cgagttgcgt ccgcttccac gaagggcggg gagcatttaa cttttatggg ggaccgcgat
86341 gcagtcaggg tatttcacaa ctttcgcctt ccccagcctg acctttctaa aagagctgca
86401 gggaggggac ttcttggcta ctgagggcaa atctctatgt gattggtcct tggctttttt
86461 cctttataga gatgttcacc caaccgagag tgtgtcctcg ggaatgggtg ttgataatca
86521 ttaagccgta attggtaact tcattctaag tgctttatac aaataaattt aggccacact
86581 gtggctctgt gaagtattgc ttttctttgt tttaaaaatg agagtgagac acagggtgca
86641 tagcagccaa ctagtgagag gtagagctgg ggttggcacc taggcagtcc aggtcagagc
86701 ctgagttgat cacctcttgg atacattctc attgaggcag aggaactagc tggactcgta
86761 gggaaagcaa gttaggacct gattgaaagg acgttgagag caaggctcgt gggcttggac
86821 gtctaggaat ttttaaaaat ttctcaacta ttttgtttca tgttttccaa'■ gtatttcgta
86881 ctgttgtgga actgtatcat tgtggaaaag tctgaaatag gagaaatgag agtagaaaaa
86941 gttttttaat tacccatgta tccaagatgt aaaggtagta actgttaagc ttctaattga
87001 tttcttttgt ctttctttgt gtctacttct atatttgttg ataaacattt ttttaaaaat
87061 tgttatattt taatgtgttt tcccatatgt cttttaacta tagaaaattt taaatattgc
87121 taatagagac taaactagtg aggctggttg tggtggctca tgcctataat cccagcactt
87181 tgggaggcca aggcaggtgg atcacttgag gtcagtagtt tgagaccagc ctggccaaca
87241 tggtgaaacc ccatctctac caaaaataca aatattagcc gggcgtgatg gcacgtgcct
87301 gtagtcccag ctactctaga ggctgaggca ggagaattgc ttaaacccgg gaggtggagg 87361 ttgcagtgag ccaagatcac gccactgcac tccagcctgg gcaacagagt gagactcaaa 87421 aaaaaaaaaa aaaaaaagaa taaactagtg aactgccata ttatataatg ctcaatttta 87481 ttattcccat tatccttatg actatttcct gatatctaaa atatttttgc tgggcgcggt 87541 ggctcacgcc tgtaatccca gcactttggg aggccgaggc gggtggatca cgaggtcagg 87601 agatcgagac catcctggcc aacatggtga aaccccatct ctactaaaaa aaaaaataca 87661 aaaaattagc tgggcatggt ggcaggcagt tgtggtccca gctactccag aggctgaggc 87721 aggagaaagg cgtgagcccg ggaggcggag cttgcagtga gcgagatcgc gccactgcac 87781 tccagcctgg gctagggagc gagactctgt ctcaaaaaaa aaaaaattaa aaactaaaaa 87841 caaaataaaa tattttcatc tttatatcat ttaaaagcta tagaaaagtt gcaaaaatag 87901 tataaggagt tctcatactc tatccaaatt tgccaattgt ttacattttt cctggttgct 87961 ttgttgatga ccatcagcca aataccacca ggaacataac taaccagaga cagctgatta 88021 tggctttctg cagcaaggaa gcccacatac catggtaaat gtggggtgtg ttggtaagag 88081 gtatttaaga agggatggta tagggtttca cataacatgc tagataatat tagatattaa 88141 ataatattgt tatctattac atatcagatc tgttaaaaga ctactagtag ttgtagtgtt 88201 actcattaga tagtgttgaa tagcatatta cctaacatcc tgttatctaa tctgtagtta 88261 catatttaaa tttgttcagt ttttcattta aagtcctatg ccaggcacag tggctcatgc 88321 ctgtaatccc agcattttgg gaggcaacgg cgggtggatc tcctgaggtc aggagttcga 88381 gaccagcctg gccaacatgg tgacaccttg tctctactaa aaatattttt aaaagccggg 88441 tgtggtggca tgctcctgta atccctgcta cttgggaggc taaggcaaga gaatcacctg 88501 aacccagcag gcggaggttg cagtgagctg agatcgcgcc actgcactcc agcatgggtg 88561 acagagcgag actctgtctc aaaaaataaa aaaataaagt ccttatgact gtgtccccta 88621 ccccaccctt cacctgcaat gccattcaaa gtccagttta tgcattgtct ttagttgtca 88681 cattccacag tttctttagt ctagaaagat tccacagtct ttgtctttta taaacatggt 88741 ggtttttttt ttaataggaa agttattttg gagaaagatg ttcatttgga gtttgatata 88801 tttcatgttt agtttcagat tatgtattgt tggtaaggat attgcacaag tgatgaggtt 88861 tcctctttat tgcaacataa cagggttcac atgatgtggt ttgtcccttc actggtgatc 88921 tcaactgtgt tcacttggcc aagatgatgc ctttcagatt tgtccgctgt aaagttacct 88981 tttttaaaaa aactcaagtt tcttattgga aggtacatta ttagtgggca ttctactaaa 89041 gatgaacttc tagttctctt catttattca tttgtttgtt caagtataaa tctcccaaca 89101 atatggactc agaattcagt cttatgaagg taaatgacat gtggattagc aatttttttt 89161 caactctatt gaggtatatt ttacataaag cattcactca tttcaggagt acagtgatct 89221 ttaggaactt tgtaaagYgg agtaaccatc atcataattc agtttagtta tttttcaccc 89281 tcagttggac tctgtgccca tttacaatta aacctcattc tcaccccaaa tctggagcaa 89341 ccactaacct acttttcctc tatgtataca tttgcctatt cggacatttc atataaatgg 89401 aatcatctaa tccatggatt ttggtgactg gcttctttct cttatgatgt ttccaaggtt 89461 tgtgcatgtt gtagcatggg ttggtacttc attcctttta atttccaaat tagattccat 89521 tatatggata tgccatgatt tttctatcca tttacccaga ggaaggcatt ttggttgtgc 89581 acagttttgg ggttagtatg aataatgctg ctgtgatcat tcataataca tgtgcttgtt 89641 gctacacagc ctccccagcc accatttcat gtctctcttt tgctgtctct ggcattttcc 89701 aggcacaatt ccaccttcct ttgtgctcca ttactcaaga cWctgaagac tccaaaaagt 89761 agtaggaaaa atgaccacat tcaaggtgag tagagctcac ctctcttgct attaaaattc 89821 catcttattg ctcatattgt catatatttt tctctgtctt gggaagactc aaggaggaaa 89881 tgggcatttg ggacctgttg tgtgccagtt gcttcctttg tctcttttKt gttgactttg 89941 catttggttt ttggtttggg ttttgttttg ttttttactg ttacattatg gagtagaaaa 90001 attagtagga atgaacagtg ctcccacttc ttcacctcct tgctctcaaa ttagcctgat 90061 ttttcttctt gtgctgttcc ttgtacattc ataactgtat ttgtattgat gatggagttc 90121 cttatgtcRa gagtgctcta tacaccaaga taacatgtcc ttctggtttt cttcatactt 90181 gtccatcaga tagttagtcc ccctttctgt ggaggcccac attgcgcatg ttggctccat 90241 ttctcttaga tgctccaggg accctctgac ccacttcacc cacctcctga gtcctctggc 90301 cccaggaatg agctattatt taaggtgtcg caatcagcgg atcacacacc aagctcctgt 90361 gtttccgtat tctgtccttc tgtctcaatc catgcagcca gctcacatct gtggaggggt 90421 ggcctgtgtc ccgcttatca ttctgtcttc tttataaatc atcaagctgc tgtccagcct 90481 gcgcccccag gctgccatca cagcactggg ccaattgccc aggatataag tgctccccct 90541 ttgccagtcc agagttctgt caaattggac cctgctggct gcaccaattc catggaacag 90601 gttcactagt tacagacttg gcgagtctac catgagagag cacactcatg tatgacaaat 90661 tacataaagc agatttatta catgtaggta gcaagggaca acagaagcct atgatccact 90721 gtgagccagt cctccaaggc ttaagaaagc tgcctagagt gggtggagtc acgactgcac 90781 ttgccccatt tgcaccatag ctgaggcacc ccaagaggca gcctgtccca ggctatatgc 90841 ctcaggggta acatgactta ctgagcagtt cctctctaac tcaagatatt atcttttatg 90901 gattggacta gaaccaaaga aggcataaaa aaaaataaaa agatgttatc ttccctggga 90961 aggacactgc ataactagca catttatggt tattcttgag acctataaac aagaaagtgg 91021 ggagaatgga tccaaggcta ctttgaaaat agttacctaa aacctgttag tttggcaaat 91081 actaaagaaa agtgatagca tccagggttt gggtctgaga gaggaggaca ataagcctca 91141 tcaaacaagg ttgagactga attSctcatt gttgctgggg caactggggc aattggcaaa 91201 attcattgaa acctatatag gtgtagccca ggatgcacca tttgaaatcc taaggggtta 91261 cttacaagga gactcagtaa gtgcacaaag taaaaataca ggaggtcttt ttcatgattg 91321 cacttctggt ggagctcagg aaaatcactg tcaggataca gacagaatga gtgggaaatc 91381 tataagttga cctccacttc tcctctccgc ctgctcaatg ctgctcctcc cccagtagtc 91441 catggtcata ggattgaggt tatgtatcct tgatgttata ggaggcagtg accttcaagg 91501 atgtggctgt gttcttcact gaggaggaac tggggctact ggaccctgcc cagaggaagc 91561 tgtaccaaga cgtgatgctt gagaacttca cgaacctgct gtcagtgggt gagcacaggc 91621 accctctgta atggtacatc aggcctcagg agtggctttg tatcctagag tattcaagtt 91681 tgtgtatgca gtgggaactt aaatttccag taaattttac cttgatatta cctagaatga 91741 attggtatta agcacatgac tttgcatgtt cacagggcat caaccattcc accctttcca 91801 cttcctaagg gaagaaaagt tttggatgat ggagacagca acccaaagag aaggaaattc 91861 aggtaagaag caagcaactg tgtgtccttg tacatgactt tctttcccat ttgttttact 91921 tctgaccatg cccacttata tcaccctgat ctaaattgcc caatctcttt cctggtttat 91981 tacaacccct ttctggctgg tggtcctgtt ccctcccttc caccctgaca tctattttcc 92041 cccgagcagc caaaatgatc cttgggaaat gcaagtcagt tttccactcc ttggtccaaa 92101 agcctttatg gtttattaat gttattaaag ttacaacctc tttattggct tttagagacc 92161 ctgaggtttg ttcttcatcc cctgctgcct cttgactaca tctgtcctct cgcccttgca 92221 tcatcttttc cagccacgtt gtcctccctg ctgttactct gtcctgtcat ggacactgta 92281 ttccctgttg ctggtaacaa ataacagact tcacagatta aacaacactt ttgttctttc 92341 tgacatgggg aggacggtct cccacccctc accctcgaaa ggagtggcca gccacaaccc 92401 taaatcatat tgtttcacta tccagagtga tccagagttg gcaccctaac aaacaaagac 92461 actgtcctcc cgcatgatat gtcaggatcc tagagattac ctctcagatg ctgaggacaa 92521 agttctgact tctttttact tagggtaact tctatataag gatatacaac ataaacacag 92581 atatataaac tattgatttt gttattgttt ttccttaaat ttccttttga tttatatttc 92641 actatgaaca taatgtttta atttagtaat ttatcatgca agatttttga ctgaccagaa 92701 aattccttag aaatagtaac aaattaaatg tatatattat atcagttttt taatggagtc 92761 tgttatttcc atcaaggatt ttacatatga gacatggtgt ggttataaat tggtcacata 92821 caaaccagaa aacaagattc atggggaaaa cataaactga acattcatta aagttgaatg 92881 ccatatccta agtgtgaaat cttaaaaaca ccaaacaaag gcttcatttg tccacatctc 92941 ttcattctgt gtccttatag gcggcaagac tattgcggaa gcaggaccac atgaagactg 93001 cccttgccag caaatctggg aacaaactgc aagtgactta acccagtctc aagactccat 93061 cataaataat tctcacttct ttgaacaagg tgatgtcccc tcccaggttg aggcaggact 93121 atctataatt catacaggac agaaaccttc acagaatggg aagtgtaaac agtccttcag 93181 tgatgttgcc atctttgatc ctcctcagca gttccactca ggagagaagt ctcatacatg 93241 caatgagtgt ggaaaaagct tctgttacat ctcagctctt cgtattcacc agagagttca 93301 cttgagagag aaactctcta agtgtgacat gcgtggtaag gaattcagtc agagctcatg 93361 tctgcaaact cgtgagagag tccacactgg agagaaacca ttcaaatgtg agcaatgtgg 93421 gaaaggcttc agatgtagag cgatacttca agttcactgc aaattacaca caggagagaa 93481 accttatatt tgtgagaaat gtgggagggc cttcattcac gatttccagc ttcagaaaca 93541 tcagataatt catactgggg agaagccgtt caaatgtgaa atatgtggta agagcttctg 93601 ccttaggtca agtcttaata ggcattgcat ggtccacaca gcagagaaac tgtacaaatS 93661 tgaggagtgt ggaaaaggct tcactgatag cctagatttg cataagcatc agataattca 93721 cacaggacag aaaccgtaca attgtaaaga atgtgggaag agcttcagat ggtcctcata 93781 tcttttgatc catcagcgaa tccacagtgg agaaaaacca tacagatgtg aggagtgtgg 93841 gaagggctac attagtaagt caggtcttaa cttgcaccag agggtccata cKggagagag 93901 accttataat tgtaaggaat gtgggaagag ctttagccgg gcttcaagta ttttgaatca 93961 taagaaactc cactgccgga aaaaaccctt caaatgtgag gattgtggaa agaggcttgt 94021 acaccggtct ttctgtaaag accaacaagg agaMcacaat ggagaaaact catccaaatg 94081 tgaggactgt gggaagcgct acaagaggcg cttgaatctg gatataattt tatcattatt 94141 tttaaatgat atgtaagttg tacatatata tgggatatgg tatgaaattt taatatgtgt 94201 atataatacg taatgatcaa attgatgtaa ttagtgtatt accttaaaca attaacattt 94261 ctttgttttg ggaaaattca aaatccattg ttctagcgat
[0381] Following is a genomic nucleotide sequence of a FOXA2 region (SEQ ID NO: 7).
FOXA2 GENOMIC REGION >20:22487401-22586400
1 aaattctctt aaaaaaagac aaatgagggg gaaatcacat aagcaagtaa ggtttcacac 61 acatggtgct ctagaaacta atcagtggac agattcagaa tccatatctc cctttccaaa 121 atgttctctg gcagaagtca ttctgtagga caaactggaa tcaaaagagt tatgaaaatt 181 gtacaatggg atgaaggggc ttcccYtgtt ggggaataga tagctgacat gctggatggg 241 gccagggttc aaacaaaggc ccctgttgag tgggagcagg tacaagacag atggtttgtt 301 aaacattgcc agcatgcttg cgcagtgccg tgcggcggtc agagcctgga taatggtcac 361 cagggcattg tgtagcagcg cattggctaa tttcagccca gctaaagcta ttgcttatct 421 ttgttggttt tgcaaagatt aaggcagttt ctctgctttg aaKgcaccac agctgcccag 481 caaagcattc ctgaagtttt gtctgaattc ccactgctaa gaggggccaa gcaaaaggtg 541 gctcgtgcaa ggacttgttt taagctcata tgtgaagttg cttctcctca actaatctgg 601 accactcttg actcttactt ctgggcaaaa ccctctagag ttacaggggc cttctttctc
661 agtgagtcct ttcctgtgtg gttcgaaatg catctgctgg ggatttatat ttcctagatt
721 tatatttcct attgggattg attcatttgc tacttttgtg gaaaaaggta aacaccattg
781 taaattccat ccggtgaatc atggggaggc ggtggttcca attaaacgtg tttgctttcg
841 gaagggggcc cttggcttct tatctttatg tacagaagaa aatagagctc aaccgggcct
901 ctgcagccaa tggaaacgca cctggcccag tcagctgcta ggctgagtct ctgctgctct
961 gggctccaac tcgctcctct cggttgtttg cagacaaagg gagagtagct gcgggtcatg
1021 cagaggggac agtccccttg gctctgccta tagtcccaaa gcaaagcagg gggtgcagct
1081 actatctggg tatgttagga aagggtgatg gctgatcggt ttagtatttc caacagttca
1141 aatgtcttta tcttctttta aaggtagtgc atatctatcc ccacctaatt gaaccagtgc
1201 tgtatttact tcaggcagcc ccctctgcct aaattagatc atttctagct ataaaatcaa
1261 gaaagtgtgc ccagcagatt aagccctaaa agtggacttg ttaactacat ctacagaagc
1321 aaagagagca agagaatttt tatatactct ccctctctcc ctctctctct cttttctctc
1381 tctctctccc tctctccctc tctccctctc tctctgcctc tctccctctc tccctctctc
1441 tctgcctctc tccctctctc cctctctctt tccctctctc tctccttctc tccctcttgt
1501 ttctctctct ctctccctct ctccσtcttg tttctctctc tctctctctc tcatgcatgc
1561 accacacacc cctaccttta gaactaggaa actaattcgc cttcctggct ccaggcctca
1621 gcatctctgc ttagcatcag gcatttgtca tatagatctc aaatcaggag gctttagtga
1681 gcagctaagg gctgacaatg tgtcccaggg aaaagaaacc cataaaggag aaaagcaaag
1741 gtctgagagg ttcacctgca aattctggac attgaaagca actgtacgtc tcctcaggtg
1801 caaataattg aaataatact actactcagg ccatggtgca gtgtgctcac ccaaggccct
1861 tcttgtttta ttttatttta ctactttccc ctggattccc tgtgtgttct ggctcccaaa
1921 tcactcactc acttaggaac ctcctggaac gggtttccta gtttgggttt ggaacctgtc
1981 tagaagcttc tattgggaga gagagtgctg tttgtgactc catgtcggca gctttccttt
2041 gattcctcta gtccaaggct gctatttttc ctaagccgtt gtccatctgg gtctaaatgt
2101 attggcgtgt agagtttcac agacactttt gccatctcat ttttcaaaga atcacatttt
2161 aatggggatt tttggcaatt tcattgattt ttttaaactc actttatatc tttttctttg
2221 ctttggtttt attctgtcat ttttcttttt ctttttctaa tatgtttagt tatgtcctta
2281 ggtatgtggt ctttcggtgt cttgtttgct atttgtgtat tcaaaattat gtaaaacttt
2341 aagtgcaact tgagagttat gggtcataaa gtttgacatt tgacactttt gttcttatcc
2401 agttccaagt attttgtaat atcttttaaa tttcctctaa accccaatgg ttatatacca
2461 tacattataa tgttttatgt gtccaagaga tggtcgaagt ggttttattg ccattactga
2521 ttttttaact ttatttcatt ataatcaaca cattgtgtat gtattgatta cttggcattt
2581 acaaaagttt tctttgttcc atgggaatta aaatgaatat tctctgtggg tgtaagttat
2641 gtacatctat tagatcagtg ttgtccaaaa gaaatataat gccagccaca tatataattt
2701 tatattttct agRagccata ttaaaaagtg aaaatgcaca ggtgaaatta attttaataa
2761 tattatttaa gacaatatat acaaaacaac aatttgaaca tataatcaat ataaaaatat
2821 taacaagatg ctttacatta tttttctgta ttgagtgtta gaaacctggg gtggatttag
2881 ctctttcagt gcatttcaat acaaatacta aaattggata cacttgatct gtatttagat
2941 ttcaatttac aattgaaaga gcagattgtt ctaaacatac ttaaaagttt ttcaatacct
3001 gaattaagta ttaggtttta aaatttaaat taattaaaat taaataaaat cttaaaattt
3061 tcttgcatta gctacacttc aagtgctcaa gagttatgta gctatcggct actgtattgg
3121 acaatataac attaagtaac tgttataatg cttctcaaat attccaaatt attggtaatt
3181 tttgtctcct ttgtcagtgt ttttttaaaa gatttattga catctctcct actattatta
3241 tatctataag accttacatt ctgaaattgt gtgtgtgttt caccccctgt ttattttttt
3301 cctctttctg ggactcctgt tatagggagc catgttctta actattactt tccgtctcaa
3361 tctcttcatc ttgctgagtc cttcctgatt tgttcttcca ctgaatccac cctactattc
3421 agtccctcaa ttatgctctt tattccaaca attaaagtct gtttgtggct gtgtgttctt
3481 gttttgtttt tcctgatctg tttgagaata tttattaagc ttcctttaat tcatgccctg
3541 cctgaactcc cctaccatct catctagtgt gtgtggctca gttattgggc tggccctgac
3601 cctttcatat cctcctgagc ccacatgcat gtggaaacac cggctgcctt ggctggtctg
3661 acatccctgg gagaaggagc aaaggcccag gtgcaggcct tgcctgcctc gtggtgagga
3721 tgagcctggg ggatgacatc ggggtgcgtg gcttccatgt ttattgtggg ctggacctcc
3781 cctgagtgac ccaccctctg tgccaggtga cagaggagct ctggagagct cccagagccc
3841 tactcttttt ctacagctgc ctgccttaaa atcatcccca ccttgcttaa gtgggagctc
3901 gggaggagag tgctttaggg ctgccagagt accagctggt tcaacaccgg gggactgatg
3961 ctttgctgag gtgatgggga agctcttatt ttcttgaggg aagttcaggg aaggaaaagc
4021 acataactcc acaaccccag ccagcctccc ttttggtacc tggccctcct gaccctaggc
4081 tatcatttcc cagattcttt ttcgagaatc agcaaccttc taccttccat ctgtggctca
4141 ccacatttgg gtttaataaa agaatccaac atctccattt tgttagttca tcttgccagg
4201 aaaagcgaac ctcataaagt gccattttaa gaatgaaatc atgtcttttg cagcagcatg
4261 aatggagctg gagaccatta ttctgagtta aataactcag aaacaggaaa tcaaatactg
4321 catgtcctca cttgtaagtg ggagctaaac aatgggccca catggacata gagatggaaa
4381 aaatagacac tgggaaccca aaagccagga gagcagaagg aaggtgaaaa ctgaaaaatt
4441 atctgtcaga tacaacggtc actgtttgag tgaagggttc actagaagcc caaaacttac
4501 cattatgcaa tatatcaacg taacaaacct gcacacgtgc ctcttaaatc taaaataata 4561 ataaaagttt ttttgtaaaa aaaaaaaaaa agaatgaaag aaaaataaaa taccatttta 4621 tttttattta cttttttttt tttttttttt gagacagagt ctcactctgt ctccgaggct 4681 ggagtgcagt ggcacgatct tggctcactg caaactctgc ctcccagttt caagtgattc 4741 tcatgcctcg gccttccaag tacctggtat taaagtgcgc acggccacac caagccattt 4801 ttttatattt ttagtagaaa catggtttca ctgtgttggc caggctggtc acaaactcct 4861 ggcctcaagt gatccaccag cctcagcttc ccaaagtgct ggggttacag gcgtgagcca 4921 ccacccctgg ccagaaaatg acattttaaa acatgtaaaa atgttggtga aaatgctcaa 4981 ttccatatta ctgtatcatt ccagtctatt tcctttcatc atgttttaac cttcccaaac 5041 tgtgtcagtg aaattgggac aatcaaggtc tctccctctt agagcttggg acgagtcaat 5101 aaaataaaac acaatgggcc ccagcacata gtaaatcttc acttaaaaag tcacaaggtc 5161 tctgatcctc agactccttg gcccacactg acttactgaa gggattaaag agatgcctca 5221 ctccacgagc tccataaatg cttctctgaa aaaaaaaaag tcttaagtaa tcaatgcatc 5281 aagttcttaa agctactgat aacacataac ctctcacagt tcacagttca gctatgttgc 5341 tcacaatgtg tcaatgagat gtaaatgtgg aaaaacagta taattgcttg acccaaggct 5401 atcgggcaat tttctctagg ttcacaccag cagcccttaa tcgatgcaca tccacaaatg 5461 ggtgggaggg aagagtcatc agggaatggc ttctcctcat atcacttctc aactttagaa 5521 atcatgatgc ctggggaacc gggtgtctga cctaggtaga tgcttttttg tgcaaaaaat 5581 aatgaagtag cctcagctct gctaactcag ccctacagca ggctggactc tggtgatata 5641 tccaatagat atcaagctta caacaatgct tagatgtgac tgagatttaa cagagttagg 5701 agtcattaat ggaatttata ttggctaacc taaaaagtaa ctcatttaaa gcattagaat 5761 ttaggatata tttccaataa ctcatttact acaaagacaa gcaaacaaag ccaatcttcc 5821 acccaagaac ttttcctaaa atgggacttg ctttgatctg cgctgtctaa tatggtagca 5881 caaatggcta atgagcaggt ggaaggtgtt tggtccaaat tgagatgtgc cttttagtgt 5941 aaaatacaca ctacattgtg atgacttaat atataacata atgtaaaata ggtctttgat 6001 agttttatat tgattacaca ctgacataat ttttggaatt attaggttaa ataaaatatt 6061 attaaaataa attttgcctt ttgtcatttt ttaatattaa ttatatttct agactttggc 6121 tatataaatc actctatact tttcaaatat tatttgttca atggaaaggt gatcttaacc 6181 aggagtttaa caaaaggcca catttctgag gcatgtttct gaggcatatc tttcgtaaac 6241 cattgttgtt gtttttttaa gactgaatct cgctgtgttg cccaggctgg agtgcagtgg 6301 cacgatcttg gctcactgca acctccacct cctgggttca agtaattatc ctgcctcagc 6361 ctccccagta gctgggacta caggcacctg ccaccacacc tggctcattt ttgtattttt 6421 agtagagaca gggttttgcc atgttggcca ggctgatctt gaactcctga tctcaagtga 6481 tctaccggcc tcggcctccc aaagtactgg gattacagct gtgagccacc gtgcccagcc 6541 gcaaaccatt ctcagacaca taaggtgact ccacagtcaa agaagttgta cccatttttc 6601 catttatccc ttagtaaagc tactgagcac cttttgtgtg tcagactgtt Yccagcactt 6661 gccagtttcc agcaaatagg aggcttgtct ttgcatgcac agaggcagcc aataagaaat 6721 aaaagtagga aataagtgaa ttttagagta tgctcaaagc caatgagtgc tatggaaaaa 6781 taaaaagtgg ggaatgaggg aggtgtgggc aggtgattcc aactgtagct ctggttttca 6841 ttaagaaagt ggcattgaag cacagacatg gagcaggtga ggtgttaagc atgctgaggc 6901 tgaggggaga gtattctagg tgggctggag gctggggatg aagttgagga gcagggagag 6961 ggtggtagat gatctcagcg ggggagtagg atgaagacac aggaagtatt actggatctc 7021 agtgaaatgg ggatgacatt gggtgctgag gtggggcgtg gctcaaacct ccttacattt 7081 ttaaaagatg actacctcct ttgttgaaaa tagagtatag gggccggtgt ggtggctcat 7141 gcctgtaatc ccagcacttt gggaggccga ggcaggcaga tcacgaggtc aggagatcga 7201 gaccatcctg gccaaaatgg tgaaaccccg tctctactaa aatacaaaaa attagttggg 7261 cctggtggtg tgttcctcta gtcccagata ctcgggaggc tgaggcagag gaattgcttg 7321 aaccggggag gcagaggttg cagtgagcca agatcgtacc actgcactgc agcctg.gcaa 7381 cagagcaaga ctccgtctca aagaaaaaaa gaaagaaaga aaatagagta taggttggtg 7441 agggtgaaaa cgaagtaagc agcaggaggc tcctgtaata atctaggcat gagatgggcg 7501 tggatcccat tggtaacatg gggttgtgga aagtggcagg attctagatg gactttaaag 7561 acagaactag cagatgtctt gatggactga atgtgtgagg tgtaaaggaa agcaagtgtc 7621 tggatgactc cagttttggc ctaaggacct ggaaggatgt aactattggg tacccacttt 7681 ttgtgccagt caacatttat gcaggaacta caggaatcca cacagtaaga aatgctccct 7741 gcaccgaagg agttcacagc aaagtggcaa agacacatga gtgagaatat gctgcaccag 7801 atatgatagt acctgtgcac agagacaggg gaaagatccg tctaacttgt ctgtgggggg 7861 caggcaaggg acgtttcaat taggttgttt ggggatgtta caaattcatc agaccaaaaa 7921 aaagaagtga aaaacattct aaggtcaagg aatagcatgt tcatgtgcaa agcccagtgt 7981 tgacaaaggt agactattgt ttggggactt gcaagtacag ggtgggtggt gggttgccag 8041 tgggtgaagg tccggcctgg agagacttaa agtaggacct gctcatgagg atctgtgcag 8101 agctagggag gttgggcctt ctcctggagg caggggagag ctatcagaag gtttcgggca 8161 aggcaatatg tgatcaaagt tgtgttttcc aaagataagt catgtggctc tatgaggaat 8221 agactggaga gaggatacat taaagatata taaaggagtt agggaacgat tacaattgtc 8281 caaggttttg ttgagcttta gctagagaga aatgagagtg agagaagtac taatcaaaga 8341 gactttttta ggtgtaattc ttacaaattg atgtccactt gaaagatggg ctttcaaata 8401 cggaagaaag agtacgaaac ataatctctc tcatttctgg tctagaagct gtcaggggtg 8461 ctatcattaa ctgagatggg aaaacaggag ggagaggcac atgctccaag aatgctgagt 8521 ttgaggtatc ttagtttgaa gccccccaga tgacgaccct gaaagaaaga ttcccttata
8581 aggagtttgt ttgaaacact gtgaggaaga agggaagcag ggcaaagaag ggcaggtgcc
8641 ccacgagatt gtggagctta ccgcagtggc ccctggagct cagttctgct gtggcttctg
8701 agagatggat gcagggaaca ggcatcagca ccacttgccc aagggagacg gagctctcac
8761 cggctctcag gaaggcactg acttctgact ttcttggagg caggatgcct gggaaatcct
8821 caggcggaaa ggaagatgct accaggagga aactggtgca gcagcatgtg tgtggaaggc
8881 aagtgccaag gggctgtgtc aagggcactg aggacatcgg ctgaaggggc ctttgagatg
8941 tatctgcagg ttgagatgct gaaacaagga gaagagaatt cacatacgac gttccagtga
9001 gaagtcaagc aggaggccat aattctggga cactagcatg tttggctaag gttagcataa
9061 gcttttcaaa ataatttcct ttctaaatat cccttgctgt gaagatatcc cctctgcaac
9121 agtaactggt gtgcgctcct ctgcctctcc ccagcagcag tgacctcgtt gaggctgaat
9181 tgaatttctg gaccaacact tctccctcaa taaaatttca ctgtgatggg caagaaacca
9241 tggagtgtta cttctacctt ttctgagagg attatttcta agattttctt tctttctttc .
9301 tttctttttt tttgagacga agtctcactc ttgtcgccca ggctggagtg caatggcatg
9361 atctcagctc accacaacct ctgcctcctg ggttcaagcg attctcctgc ctcagcctcc
9421 caagtagctg ggattacagg cacctgccac cacacccggc taatttttgt atttttagta
9481 gagacggggt ttcaccatgt tggccaggct ggtctcgaat tcctaaccaa gggtgatctg
9541 cccaccttgg cctcccaaag tgctgggatt aaaggcatga gccttgtgcc cagctggggt
9601 tgcttttaag aaataaaatt ggccggactt ggtggctcat gcctgtaatc ccagcacttt
9661 gggaggccga ggcaggcaga tcgcctgagg tcagaagttt gagaccagcc tgtccaatat
9721 gatgaaaccc catctctact aaaaatacaa aaattagccg attgtggtgg caggcgcctg
9781 taatcctagc tactcaggat gctgaggcag gagaatcgct cgaacacggg aggcagatgg
9841 aggttgcagt aagccaagat tgcgccattg cactccagcc tgagcaagaa aagcaaaatt
9901 atgtctcgga aaaaaaaaaa aaaaaaaaaa aaaaaagcaa ccccgctcta ttaaacaggt
9961 agagtccagc tttctcaaaa gtgaagaata aattttgtca taggctgggt ggggtgactc
10021 acatctgtaa tcccagtact ttgagaggac aaggcaggag gattacttga gcccatgagt
10081 tcgaggccag cctggcaaca tacggagacc ccatctctac aaaaaaataa attttagaaa
10141 ttagccagat gtggtggcat gtgcctgtgg tcccagctac ttgtgaggct gaggtgggag
10201 gatcgcttgc accctggagc actctagcct tggcaacagt gagactctgt ctcaaaaaaa
10261 aaaaaaaaaa aatctgtcat ggactagcct tagaaacatt cttacattca tccccccact
10321 ccccgccccc gggctctgtt gccaaggcta cagtgccgtg gcaccatctc cactcactgc
10381 aacctctgtc tcctgggttc aagtgattct cctgcctcag cctcccgagt agctggggtt
10441 acaggtgtgt gccaccacac gcagctaatt tttgtatttt tagtagagac gaacttttgc
10501 catgctggcc aggctggtct cgaactcctg acctcaggcg atccgtgcgc ctcggcctcc
10561 caaagtgctg ggattacagg catgagccac tgtgcctggc ctagaaacat tcttcaactg
10621 gagttggaat ttttgcttta gaaggaaagc tccagagact ttattaaaac aaaaaccaaa
10681 agataaagaa agttgagaag atcctatgtc cttcatggca ggggccagtg ctatcaggga
10741 tttggaaatg gagtatacag tggaagtaag cactcctgtg ttttcctttg gccaaaggca
10801 taaattctca gtctgtcttc tatatttatg agcccatcag cctaagaaca atatcaagac
10861 tatgtaggca tagagctttg gaagtatctg aggctctggg taagttctaa tatcctctcc
10921 atgcagttga cagtgtgccc tgggatgggt catttgatct ttcaggatct caacttccat
10981 atctgtgcag tggtgaacaa gacccatctc acaaatatgt caaggtagga tgggatagga
11041 aatgggaaac tgtcacagat gcgataaggc tcttgacagt ggagaagatg tggtttagaa
11101 tgcctttcac tgttttctca accctgggtc attcttaacc ttcctaaggc tctggttgca
11161 aagtgcaggc gatgacccta accctatgaa atgcaggccg ttcactcctg gggtcccatg
11221 gcatgtggct cagagtgact cagaaatgct agagggccca gtggagtctt tctttctaga
11281 aaagccttgg atcttggaga ggccctggtc tctggttcca cttggacctt ccacaagtgt
11341 gtagtcctag gtgggttata tcacccctct gtgcttcact tactgcatct gcttcactta
11401 cttctctgat tctatgctta agaatccgga cttggctact tcatcctctt cgttactctg
11461 gttgactcag gaacaggtgc tagaagcttc atgagccatg tgctcctaat gagcgctcac
11521 tgcccgccct cagagtttgc cagcaatccc agccccaagc agctgaccct acaccctaat
11581 ctcaccttca gctttcagtt ctcctgttgg cgagccctgg agcaggcgga agaaacagcc
11641 atcttattgg gtgccacttc ccaatgtcaa tcggatctcc tttaaactca gctatggtct
11701 gcttcatttt gaacttaggg taaaattcaa gacagttcag caaagaaaat ggatgaagga
11761 cgcaaccctt taattcagtt cacaacccct gagggtggac agtggccagc cacatgctgg
11821 cccctggaag gagtgaagga agtcgaacac tgtctagcaa aacaaagcac atttggaaaa
11881 taaatgtcat ttaggataca actaccaggt gcacacttct ctttatgttt cagttcactc
11941 tgccttgtac tttaagacac gtttcctccg ctacattgac ccacctccca cctcggccga
12001 ggaaagcaga tgccacgtag aatgacggtg attcctgcaa gctctatgag tcagggacat
12061 atggtgtccc cacgttccat gtttactctt gtacctggag agattagtca gccagaagca
12121 cagaatcagc ctgaatgcca tctggccctt ccctggagca ggctgaaggt gaaaagtcag
12181 ccctgcatac cttgatagct aggtgagggc tgtcaccaat gggggtggaa gtatcatctc
12241 atgttgtacc cactggcaga ctcatttctt ctgagcaagg gagaactgca ttctggagaa
12301 aggacactgg ccattcaggc tgcaattcgg gttccccagc cctcctgtgc cgtcctcttc
12361 ccagccatga gacacccgct cctgaagatc ccctttcccg tggtcagccg gaggcccggg
12421 aatgagtcta agagtcattg gtattctgtg ttttagctgg tgctttgtca atgaaaacat 12481 tctggacttt ggccccacat ctgcctgctt ttcagctctg gtcctgaggt cttgggtaac
12541 cttcccaacc tccctaaatc tccctttctt tgtttgttaa atgacaatcc tcatagggtt
12601 gttgcctagg attaacttat ctgagataat ctatgtaaag cacctggatt taggagaaat
12661 gcaataaaag ttagttccct tcccccttgc cttggattac atggttttat tctcttctgc
12721 cagagattcc ttggggaaag gaaagcgatt gtgtaatttg ctgtaatcct aagtgacgaa
12781 gtaaagttct tatcaaaata attttgatgt cccattggca tgtctgggcc acggaaataa
12841 tgacatgatg aaaattaagt tgacgctatt ggctgacaga aagagcttag ctagtcagta
12901 caaagatagc tagactggca cccaaggagc cccggatctt cccaacaagg agaaaaatta
12961 cacccagctc tctggggagg cactggactt ttgtaggcct acaagttctc ttgagtcacc
13021 agcattccag gttcactccc tgggggctct ctaatctgtg agacctggct atggttctta
13081 gcccaccagc cccaggagaa agacagtttg gcagtggatt ttataaccaa tcccatacat
13141 ggagcttttc cataatttat cttggggcaa tatattaagc cacaagttac gaagttccaa
13201 attatgaaac tgtctctact gaatttccac cagcccaatg ggtgtcattg taggatttat
13261 atttttttct gggaagaatc atgttgtctt taaaaaataa aaaataaaaa aaaagaaagc
13321 ctctgcttcg aatagctatc tctcttgcag ccgtgcactg ctggctgcac aaggtcctgg
13381 gtgctgttct ctgggtcaac ttccctgagt ctgtttggag aaattgtgac atctaaccaa
13441 cttcagagaa atcgatgagg gctggaaggg tggactttgt tcccctgtgg tgttctaatg
13501 aatattttca ggcttaaata actgcccaaa aatcaaaatc attgattttt ccaaagggca
13561 gatacaagca gatggcccca tcataaaggt acttccaatg gttttccagc actcactctt
13621 tattcttcat gttgaacaac tgcaattctg gagactgaag aatggaaagg aggaggctgg
13681 gtacagtggc tccctgctct aatcccagct atttgggagg ctgaggcagg aggatcactt
13741 gaacccagaa gtttgagacc agcctaggca acatagagag atctcatctc tacaataaat
13801 ttaaaaacta accaggtgtg gtggcacacg cctgtggtcc catctactca ggaggctagg
13861 atgggaggat cacttgagcc tgagaggcag aggttgcagt gggctgtgat tgcatcactg
13921 tactccagcc tgggtgacag agcaagaccc tgtctcacta caaaaaaaaa aaaaaaaaaa
13981 agtaaaagaa agaaagagga aggaaggaaa agtcctgagg ttgcctactg tggcacatgc
14041 aaagggcaga gggcatcttt ttgcagcaaa agctgtccag ttatagaagg catcttggaa
14101 gccttatgcc cttgagtgca gcctccttgg tgagtccctg agtcaggtgg atccccatga
14161 tcggactcta gggtttggct tgtgtttccg caccagcaaa gagttgctgt gaccaggacc
14221 gttatgtgca tgcagaacaa gagtggcgcc agagtccaaa ataaaaccgt gctggagcag
14281 tgccagagtg cataagcaca gtctctcctt gtagccaagg tgggaaccac agctgggtaa
14341 agaaagggtt tcagagtgga gaagagtggg ctccagccct gaccggatct ctcctggatg
14401 agttcccatt gtcttactta cttcaaggtt cgattttttt catccataaa atggaatgac
14461 aatgtctctg ggggctgtgg aatttcagca tatgttcaac gtgttatgag aacaaggata
14521 gaggcagttt acttgttcct atggagaaac aaggaaaagg aatttggggc cgggcatagt
14581 ggctcacacc tgtaatccca gcactttgag aagccaaggc aggagaattg cttgagccca
14641 ggagtttgag accagcctgg gtaacatagt gagaccccat ttgctacaaa atataaacat
14701 gggagaggtg ggacactcct atagtcacag ctactcagga gactgagggg aggatcattt
14761 gtgtgcagga gattgaggct gcagtcaact atggctgtgc cactgcactc cagccagggc
14821 aaaggagcca ggctgtgttt ctaaaaaaag aggaagaaag agagcagtgg gtgaggggga
14881 gagagataga gagagagaaa gagagagaga gagagaatga aaaagaaagg aagggagtga
14941 gggagggagg gaggaaagga aggaaggaaa gaaggaagga aggaactaaa gaaggaagga
15001 aagaaggaag gaggaagttg gggaagcaca gcctgtgaaa tttgggaaag attttttgcc
15061 tcaaatcccg gggcactttt ccatgtcgga tgtgaacacc cagccctact tctgttgtga
15121 tccttagcat tgaaacctgc ctccccaaga agagaaattt ttattttgag cattgagcta
15181 ttctagagaa accactatta atgacgaaaa atcacatttg agccatgagc aacatcttgg
15241 gcctttggta gtcccagaca tgagacttgg ggaaggtggt ttgggggtta cttttccagg
15301 gtaaatccct tgtcctccag aattgccatt cagacataat taatattctc cctgcttctt
15361 ctttccagaa aaaaaaaaaa cccaacacat gcttacactt acttgttggg cctaattgtt
15421 gtccaaatta gtcctacttt ttcagataaa ttgtgtcttt agaaaagtgt tgaaattggg
15481 tggagctctt gagcaccctt tgtggccggt ctttcaccct gacattggca tgtgtggtac
15541 cggccaccca tgacacatct cctgccctcc tgccctgaaa aggttcaaag tcacagacac
15601 actgaatcag agcgctaaaa gggctataga aaacctgcaa taggctgggc acagtggctt
15661 atgcctgtaa tcccaacact ttgggaggcc gaggcgggtg gatcacttga ggtcaggagt
15721 ttgacaccag cctggccaac gtggtaaaac cctgtctcta ctaaaaatac aaaaattagc
15781 tgggtgtggt ggcatgtata atcccagcta ctcgggaggc tggggcagga gaattgcttg
15841 aaccctggag gtgaagtttg ccgtgagctg agatcacgcc attgcattcc agcctggaca
15901 acagagcaag actctgtcaa aaaaaaaaaa agaaaagaaa agaaaagaaa agaaaagaaa
15961 agaaaagaaa acccacaata agtggggatg tgatgatgta ggttcaggtt ttctcacagg
16021 acagtcgact ttgttaaggg gagaggaaaa ggggagatat ttctgtaaaa gtgtggttca
16081 gctgagagct tcccacagtt aatgcagcgc cacctctttt gctgtataaa cttacacagg
16141 ccccctaacc tctctggtca ttgtttcatc acctataaat atacaaataa tatctgataa
16201 cacctgcctt gtctataact tgaagatgag gatcaagcaa gaaaacagat ctgaagacat
16261 tttgaaaaat atgaaagcta taacatatta taataataaa ttaccatgtt tattatgtct
16321 actacgccca agctgaaaaa ttatacactt aaaaaaaaaa ttttcttttg aactgtccac
16381 agagccaggg atgacacaca tttctctact agttttctgg tcctcatttc aaacatacat 16441 ttgagctcca aagtcatcct gaattattat atgagtgtgc attataaaat cctgtcaact
16501 gctctgtgtg tttgaaattt ttcttttctt tttttttttt tttttttgaa atagagtctt
16561 gctctgtcac ccaggctgga gtgcagtggc gtgatctcag ctcactgcaa gctctgcctc
16621 ccaggttcaa gcgcgtctcc tgcctcagcc tcccgagtag ctgggactac aggcgcccgc
16681 caccaacccg ggctaatttt tttgtatttt tttttttagt agagacaggg tttcactgtg
16741 ttagccaaga tggtctcgat ctcctgacct catgatccac ccgccttggc ctcccaatgt
16801 gctgggatta caggcatgag ccgctgcgcc tggcccaaaa attttcataa taaaatattg
16861 gaaggaaaaa tcaccttaac tgaaaagtgc agtcttgcac atgtctggtg gcaatacatc
16921 tagttggggt ctccattttg gaaaataaat aagtaaataa aggtagcatc actaaaatgc
16981 aaaaccaaac aaacaaaaaa aaaagataaa aaaatctgac ccagtaaaac atRttatagt
17041 ccagtgatgt ttgttacagt gtaattttaa tagggcacca ctctccaaaa acagaagaat
17101 gttaatttgt taaggcacat tgccttgagg agatactctc cagtcattaa aaaataggtg
17161 gaaagatgat atagcaatct tatgcctatt aaaatgctcc gtaaaaaaag caacgaagag
17221 acaagttctt atgtactggt tacagaagtg ttaaatccta aggaaaacag aaagagtaga
17281 agtacccaaa agagctgatt ggatcaggga ctgggattat aggaagattt tttattttct
17341 cttatgtaaa tttttccttt gatgtggata tacactatat ttacttttta aatttaacaa
17401 aagoaaaaac aaaggatttt actcttttta cttagtgtca tagaaggctt tatacccagg
17461 gccctcagct ctgcaatgct ggcaagaaca gctcagctga gaacactgac ctgactgggt
17521 tgccaaatgc attgttcgat tgcgcctgca gcaatgactt tgaacacaat agttgccagg
17581 ttaaagagtg cccgctgggc agtccctacc cagccctgcc taaagcctat tgttgcctcc
17641 agcacttctg caaggtgctc acatggccaa gctttgaagt tgggttcaca gagcctgttt
17701 cctcagagca tgaagtttat gttggagacc acagacttgt gggggtggga gaggggaaga
17761 gaatcgaatg tttcaaaatg aacccgaatc aaaatgatcc cccagaaatg tgtcaggaag
17821 agctaaagac cacttgctga gggctggatg ttagagataa ggaaaccgag gcagagtcgg
17881 ggcatttatt ttagaaatcg agccagtggc caggtccccc agcctttgat gaactaaccc
17941 ctttcttcca cctaaactgc ttcacacaac agagccgtaa attgccctgt gttcccagag
18001 tcagggtcac atccagatga tcacagcgaa aactcctggc ttctcaactg gagctgggca
18061 agacggcaga gccataattc aagggcagtc gtgtgctgtg aactttgtca aggttgccac
18121 cctgaaattt tgcctctgcc tgctccaggg ttgccaatga cctcaaattc ccatttctgg
18181 ctcaggccac agacttcttg gggagaagtc tcggtactca tttgaatttt ggaaaacctt
18241 agattcttcc agggtactta actcaaagat ttagcaacaa tatttcttga agtcctttta
18301 tggaccagga gctgaggtgg taaacataaa taagacacat tctgacccaa caggaattca
18361 tggagacaga agccctgtag atatgcaata ctactgctaa tcagcatttg ttaggcactt
18421 cctacttgcc aagctctatt ctatgagctt tttctaaagt aactcattta atcccccaca
18481 aaagctctat gacacaggca ctattattat gatgtgtatt tctcagataa ggaaatggag
18541 tcacacagaa gccagcacag gcagctggct ctagaccctg ggatcctaac cactactcct
18601 cactacagac tttttcttaa tggaagagaa aacctcttcc tgtaatccca gtgccttaag
18661 ccaatcacag tgatgttttg ggtgcttttt cctgtatctt ttttgcacat ctttaacagt
18721 tgtaataata aaacataata atttgccttc tttttgtttg tgtcactgag atttatagca
18781 agaacaactt ttctatatca cctttataat cctcactttc tagtgagcat taaataagga
18841 acttaatcat tatcctaatt ttgagaaaat gtttctttat aaaaaacatt gtaatgaaca
18901 tctctggctc taaagaatgt ttcttatatt cgatttttct caagcctgaa ttctcagagg
18961 taaatttaca gagacaaRat gtaggaactt tttttttctt gttaattgtt ggtacatact
19021 gcaaattttc ccccaaactg tactacaagg ctgaatgttt tgtcaccatY taatcactta
19081 tcaagcagag ttttgaagaa tgaataaagt atgtgagtga acaaaaatat aagaagagtc
19141 catgctgatg gaagggagat tataaacaga atggcaagtt catggaaccc ctggaaaata
19201 agcagggaat tctgggatgt gaagctggag gaagcagaga tgagatcgtg caggggcttc
19261 ctcacactcc atgttgaaga acagggacta gattctggag ataaggggga accctcccaa
19321 aggtgccaaa gagagcctgt aaggtcaggt ttgccagtga ggacagatct ccatggaagg
19381 atgagttggg aggctgggct ggtgggtggt ggtgagaagg cagtgcccca caggaaacag
19441 accagcagcc actgaggagt ctataaggaa tgagggcagc ggagatggga atggagaagc
19501 tgcgtccggc cagcatggtg ggggatggtt taaacRagga tggaactgac taggagggaa
19561 gaattcagca tgtctcccac attcctgcac tggatgtcac ccagctcaga aacggcaccc
19621 acatgaaagg gaaggcttac agatgggtgt gggagggtgg ttagctgagc ttgaactαgg
19681 ggcactgggR gagtccctca gtaggaatac ctgatgagaa gtagggctaa ctgaggacct
19741 ggagccaaga gagggcctag gaaaagggct tgactagaaa gctaccactg ttccggggtg
19801 agctgaagct ctgaRaggaa catggtgacc cagggagaat gcgccagagt gggcagaagg
19861 tggacagaaa tgcacccatt tgccaacagg ataggcgagg atatgtgagg ggaatttggc
19921 aaaaaagaat gagcaggaga agtggcctcc agatgacacc gtggaaccag gtgcagaaaa
19981 aaatattttt ttaattactg aaaaatgtat tctgtgacca caggagcgag aaagagagga
20041 agagaggtga atgaagaagg gagggaggga ggagagagaa aaggaggaag ggagggagaa
20101 gggagggagg aaagaagaca aaaagaagtg gccaatcaca tcaaataagg cggagattgg
20161 ccaccatccc aagatgaaca atatttgaca atgatttcac aactccctga ctcttagttt
20221 tcttatctat atagtagaaa ctgttactct gtcttgacca aatttttgtg gggtttgatt
20281 gaaaagaaaa ctacaagagg gaaaatttcc tgtaagccag tattttcaaa agtgtatttt
20341 atgaaatatt ccttagggtg ttgttgttac atgaatgaga gtttctgtgg cctcataaaa 20401 ttaggatgtg tgttaggtag atttctttgc tgcatttcct ctgggccttt aagggactca
20461 tatgcaaagt cagtttccaa gaggggcatc cagtgtttcc caaacacatt aagctgtgga
20521 accttttttt catggagcac cttgaggaat cagtgtctgg aaaacccact ctgggaaaga
20581 cagtacaatg ggtttaatga tgcccacctt atcatgggag gattggagta cataagcaaa
20641 gtccctggca aagggttggc agtcagtgga tattcatttc ttctttatat caaacaatga
20701 aagcagggga atcattctag aaaaccaaag ctaaaatgca caaaaatctt aggagataat
20761 ctttgaccct acattctgta gcctcttctg agaagcagct gtgatatggg ttcagatatt
20821 tagtctgcaa cttactagct gtgtgaactg gcaggagtta gttgatctct ctgaaaaatg
20881 atgcaggaat aaaatccgat aatctagtgt gtgccatcag ttacttcttg ctgtgtaaca
20941 catcactcca cctgtcagtg gcttgattaa catacattta cgtcacagtt tctgttgcaa
21001 agcaggcctg cttagccaga tcctctgctc catggtgtct catatcaaga tgatggccaa
21061 gggtctcctc tgaaagctca actgggaaac aatctgtacc caagctcact gacgtggttg
21121 ttggtaagag tcagtttctS aaggcctcag tcccttacag gctatgaact ggaaactgcc
21181 ccagtctctt cctgccctct cttctctaac atggcagctt gcttcattaa acagtgagag
21241 agagagatag agagacagag agagaatgca ctagcaagac tgaagtcttg tgtaacctaa
21301 tcatagacac cccctcaatg ttcccatatt ctctttttca gaagtaagtt actcaaaagg
21361 aggatactac ccacggctgt gaataccagg aggtagagat cattggtggc cattttgcag
21421 actgcatgtc acatagtgag aaaaattttt gcatggtaag tccctataga gtggctggct
21481 attcttattc ttttgaaaat cctatcaaaa ttggttagtc agtagtactt gctaatttat
21541 caccctagag agacaaaaca tccaggaaaa tgccagtggg ggaagaaaaa acagaaaaca
21601 gagtagggga cttgtcattg gtggcctgtg gtgtacaact tcttgagaca atcttagact
21661 gtgactgcag gatcccagaa ggcccttgct ccacagcttt ttaccctcaa ggagccattc
21721 ttagaattac accactagat atcctacttt tgaaaggcaa agataataac agctcattgt
21781 tttaagcatt tgacacatat taacccattt aatcctcaga gctgccttgg gagttaagct
21841 ctattattcc tatttacaga tgaggaaact gaggcataaa aagatcacat gactagtgag
21901 cagtaaaata cacattccaa tccaggctat gtgatccccc aacttcatct taacctttag
21961 agtttatggt ctttcaagat ttggaatgca tcgtctctgg tccctcaatg tgaattgatt
22021 aaggaaacca gactccagac acgccctaga gatttggggt gggagaagaa tgagaagtaa
22081 gagcagcaga gcaaaaccct gatggctttg cctagaggca cttgcatcca ttctgcatgt
22141 ctgtggatgg cagctggcca aggctagctg ggttcctgat aaaaattata gggcgtcagg
22201 gtcccggtta cccctttgtg cgctcagaag aatttcttgg cctctctgaa cctcacagta
22261 gaaaaggaag tacttaaagc tacaaaatcc ctaggagcct ttcttatgat ccagagtcca
22321 caaataactg gggctgggtg accttcagag gttgaRctgg ggagtgagct gtggcccagt
22381 ctgtagagat gacaatggac cttttcacag actgagggcg tgtgtacKtg cagctcacag
22441 ccaggacacc tggcagcatg catgcctgct cctgagctta gacaagcctg ggccctgcca
22501 cgtgagcttt taaggacacg ataagataaa atgtcttcac atggcatttg agggaccttg
22561 gttgtctcct tactttctcc acccttacag agagaggcaa tttacagtgc agaaaacaga
22621 aaaaacttaa gctttttttc caaatgagtc ctggtggtca caactcatag actatgaaag
22681 ctgtcccagc tctctccagc ccagttctga agacagtgat gWtggtcttc gttaacgggt
22741 gagtttagca agagacagaa ttctgtacac gtggctgaag aaataacttg gatgaagagc
22801 tgagaatcca agaccccagc ccagattacc cacttcatgg cagaggagag gccggcagag
22861 gaagccacca gggcttctgc cattgttgcc ttcactttgg ggccaattaa ccactaagcc
22921 taattagctc tgccaagaac aaaggccagg ggcagggaaa gaacaaagcc ttctccaggg
22981 acttgaagtt attctccagg cctaatgttt ggaaagccag ccagaaaggc cttgtgtcct
23041 gcagaactgg aatccagctc ccatggggag ccatcgcatg agttgtccat ggcattcttt
23101 gagatgaaga acatcgtagc cacactcgca ctggtggcac acacatcacc ataatcatcc
23161 tgctgccttg agacacagtc cctcggttac ccaacgtttc tgtgacttct gagagtgatt
23221 ccagtgacat ccagggagca gcagggtgtc agtctggcat ctggaatcaa ggaaatcaat
23281 agactaagtc ttcctgctcc aaatgacttc cccaacccca aataggcagc ctgaatttta
23341 agtcagtgtc ttctctggtg tttatatctc tgaagggaaa agaacaattt taagaagttt
23401 tctgacatca gaagctcctc tctcttttcc taaacttggg agtctcagtt taattccaat
23461 ttccccattt cctatctctg tgatcctaga ctagatgctc aacctctctg ggcatcagta
23521 ttattctata taaagtcagg gtcatcacac aaattttact ggtttgtttt gagcaccaaa
23581 tcgtataaag tatacagtgt gccttggttt tgactgtttc tgaatcattt ctgcaatgat
23641 tctcaaaggc attgtggcct tgccttgtcc agtaagttga gtaagcgtcc aggtttccca
23701 agtctcccct tttcactatc tgtctagtat cccccaatga ccctcacttt gactcactca
23761 tgatcactgg ggataattgc cagtatggcc cagcccctta cagttttcgc cactagctgg
23821 cttaccccac ggagcaaagc gtgataactt ggctgattca gaataacact gctctacttg
23881 ctctgttcaa ttttgagacc tgttttggaa tttagacact tctggtaaac catgtgtggg
23941 gtctctgctc cctgcatcca tgcctcccac acttgggcct gaatggacct gtggcctcga
24001 ttcatgaccc tgcatcttcc ctcctgctca tcttgctagt gtctgcttgg cttccccctc
24061 cagcctccca cgctcagagc tgattcctat tggagatttt ataactgtga cccagagaat
24121 tcagaggctt atagaggctt ggaccagaga agggtcttgg atttagaatt taagcctaag
24181 tcagtctaag ataattccgt ttttaccagc tatgtgaatt tgagcaagtc ttgcaccctc
24241 tctgagcttc agtttactca actgcaaaat attttgtatt atatgagata acatgctttg
24301 gatgtgttgt attaaatgag ataacatgct ttgctctaca cagcagcctt aaatgttgct 24361 caatacacat atatatgttt gtatttcttt tctaagctaa gcatttattt gactctattc
24421 cctctgttct cattctcccc tccagcctct gccaccagcc acctaacccc accaaaggtg
24481 ccaatggctc acaatagtct gattcagtgg ccctttctca gtttctctct ggatcttctg
24541 aaactgtcca gcaccctccg tttctcctaa aggtctcctc tcttggcttc tgcctaaaac
24601 gttcttgagg tttctccatt ccctcctttc tggaagaccc ttctctgtat ctgtgtcagc
24661 cacttgccct cagccttttc tccatcacct gtggttcctt ccaggcccct tgtgtccact
24721 gtctaccctt gttcttatca cagtgacaac tgtcaaggtc attgaggact tactctgtgt
24781 caggcactgc tctgagcact ttgcatatag atatccattt cactcacaaa taaggtcaca
24841 gatcctttca ctttcctttt gcagataagg aaactgaggc tgagaagggt taagtggtgt
24901 gcctgaagtc gatcactcag ggaagcatat ataaacaggc tgtctggctg gctcttaacc
24961 aattgtctct gagtgatctc atccccactg aaggcttttt ctaccatgct tccaacatct
25021 gccatgttgg cctcgccagc tcgacctttc aattagattc tagacctgaa tagtagttgc
25081 cttcagggoa tgtctacttg gacggcttac aggtacctgg agatgaatct gaactcattt
25141 tctttctcta caaaccaact ccaccctgtc ttctcccacc tcacccaagt cagacacctg
25201 gaagtggcct gtgcctccac ctcttatcac ctctgacagc caaccaagcc actagtgctg
25261 cctcttgaat gttcctcgct tctgccctct atgcttggtc cctctgtcat tgtctcactt
25321 cccacaggct ccatttcttg ctttggttcc tgcaactccc ctaactgctc tccctgcctc
25381 cagtaggacc tcctgcaatt tattgtcctc ttcagtgccc aggtgctttt tctaaaattc
25441 tgtgctcttt gcttaagact cttctctggt taatcattac cttagagata acatccacat
25501 tccatatggt gcgatccaag cccatttatc atctggccct gctgtctctc aaatctgatt
25561 ctccaccacc cctgacccca ccctctgctc cagactaatt ggattgcttg agttctgtgg
25621 ataaatataa aaacaaccag cactgggtgc tcactggaag ctacacaaat ggttcttttc
25681 atcattacaa ctatctttga tggagaaatt attaatgcct tgcaagtgag gcaactggag
25741 tccgaagagg tcaacacatt tctcagaatc cttcaaaacc attctgtcta gattcaaaaa
25801 ctcatgttct tcaccaccac actctaggac actggctaat tgctcaatgc Rgaccacctt
25861 cttcatcttc tttcatccca ctcggttcac tgatttcttt aaagcttagt tcaaggctca
25921 gctctctgga gagaagtgct tggcttttgg cctctgagtt gactcatgtc cttattagtc
25981 ttgcatttat cctacttctt tcagttgaaa attccatcgt ttataggatg catctttgac
26041 ttaacatcat ctttgctgta aaacaagcaa aaggcccact ctggtaaatg tatacagtct
26101 caatccatat ctcactctat atMtattaat accatataca tatgtacaca ggatgactcc
26161 tatgcaaata actaaaccct acattcagtc tctactcact tgactatatt ggtccaattt
26221 ccagttctat taggctaagc ctctagagga attattatct catgttgata tcaacagtgt
26281 ttgatacata gtagaagccc agtgatcatt cccttgaaac ttgaatgggt aaataataag
26341 tagcaagaaa agcagctagc agtttttgaa gcacatgcaa agcaccaagc attgagctag
26401 gtgtttgcca tagatcacct tattttaatt gacgacaata acaacttatg agacagggag
26461 ttttcttatc tatgtcttac agatatggaa acaaaaatca gagaggttaa gcaagtcact
26521 taacatccta caaccagagt gatacagaac taaaatttta caccagtttg gccgacatca
26581 aagtttgagg gttttttttt tctttctttt tttttttttt tttttttttt gagatgggct
26641 gtcactctgt tacccaaRct agagtgcagt ggcaggattt tggcttggaa tctgggctca
26701 agtgattctc ccacctcagc ctgtggagca gctgggactg aaggtgccag ctaatatttt
26761 tattgctatt tgtagacatg gggtctcgat atattgccca ggctggtctt ggactcctgg
26821 gctcaagcga tcctgaagcc tcagcctccc aaaatgctgg gattacaggc atgagccgct
26881 gtgcctaacc aacactaagc tgcctttcca aataataaag cctctttctt gctcatcaga
26941 aatgatggtc aggttcttac ttccctaacc ttcctgccct ccacccttag gccaaaccta
27001 cacagatcat tctagttctt cttctttttc ttcttcttct tcttcttctt caacttcttc
27061 ttcttcttct tcttcttctt cttcttcttc ttcttcttct tcttcctctt cctcttcttc
27121 ttcttcttct tcttctgctt cttctccttc ttcttctccc tcttcttctt cttcttcttc
27181 ttctccttct tcttcttctt ctccttcttc ttcttcttct tcttcttctt cttcttcttc
27241 ttcttcttct tcttcgtctt cttcttcttc ttcctttctt cttcttcttt cttcttcttc
27301 ttcttttttt tgtcagtatc cctcttttta aaaaatcaca caaaaacttg acagaaattc
27361 aagcatagct tgactagtat gaaaaattgt aggattagta tctcctttga cccatatgcc
27421 acaatctcaa tctattctaa catcttagat atgctgatgg tgtttagtga atgtgggggc
27481 caccttatcc tctaggtctt tctgattaaa actcagccag ggttcattag tcttctattt
27541 ttgtactcct ttcatttgaa gtctctcgga aaaaaataac agcaaagaaa ttgccatata
27601 taaacatatg caacttcaat ctatacttca aaccatacac aaaaagtaaa ctcaaaagag
27661 ataacaaact gaaatgtaaa attataaaac ttccagaaga aaacataaga gaaaatcctt
27721 gtgacttaga caRatattta ttaaatacgg catcaaaaac acatgtaaaa aaattgataa
27781 attgaacttc accaaaagta aacacttctg ctcttaaaaa aacactgcca agaaaattta
27841 aaaaaagaaa aaaaaaatgc catagactgg ggataagggc aatatttgcc aaacacagat
27901 aatgagctta cctagaatat gtgtttaaaa acatacaaac aaaccaatta aaattttgaa
27961 cactttaccg aaaaaaatat atggacaaat aagtatatag aaaaagatgc ttaacatcat
28021 ttgtcactag ggaaatgtaa attttaacca ccatgaaata ccaatacacc cataatggac
28081 tggttaaaaa aatgttgaca ataccaagat atgaaactat catgcattgc tggtgggagt
28141 gcaaactggt acagccactt tggataatag tttcgcagtt tcctaaaaca ttaatcatac
28201 acctacccta ttgcccagaa atgttactcc tttttgccca ggagaaatga aaacatatca
28261 cgtgcaaact tatatattca tgtttgtaaa ggttttattc agaatcaaac tgaaaacaac 28321 acaaatttcc tttacatgat gaatggatga acgttctata gtacattcct acagcagaat
28381 actactcagg aataaaaagg agcaagctaa taatacatgc aataacatag attaacgtca
28441 aattcactaa gcaaacgaag ccaggcacag aaaggctaca taaatataat tttattcata
28501 tgatactctg gaaaaggcaa aactagaaag atagaaaatg gctcagtgac tacaggggct
28561 ggcaggggtg gtgggtgtgc aggcaggagt ttgaccacta agggaagcca gggaagtttt
28621 gtggccagtg aaattgttct gcaacttgat ttggttgttg attacaagac aatatgcatt
28681 gtcaaaactc atgaaactgc aataaaaaag gaaaacttta gtgtgacgta aattccttct
28741 caacaaaaat tgcattcttc aatagttgaa ccagtagtgg tgggaaaata caaggtaaca
28801 attagttgaa cctttatcta gaaacagata gatttaccag tatctacaac tattctatgg
28861 attaactagc tggttacatt tgacatagat ttgaagagac atctaaagtt cagaaacaat
28921 aaaacatgga ggaatgcctc ttagaatcaa gcctcttaaa tacacaagtt aattttgtga
28981 tgctcaatgc atactttaat gtggccttgt ataggtctgg tttatgaaga ttttttttga
29041 ttctgagacc atgtttgcca gctctttcca atcagcatcc ctgaaagtct ggcgagcatc
29101 ccttctattt cttcccctaa gcagaacggg aaattattag ggcagctctt gtggcggaca
29161 gcagggtgtc catccgagtg gatgctttcc agcctttgtt ctcctttggg aattttgcaa
29221 cggatggaga acaaatgcag cgtcagggct ttcgtttccc aagctgaggt gagatgtggg
29281 cctcccttaa gtgtctcagg tcctggcttg gcaatgcact tgggcacggt gtgtctctgc
29341 tggtcttctc actctgccag aagtggaggg tcagccctgt ccagacaatc tgggttaaaa
29401 gcacaatcgc tgagctcagg acatgcatgt cttgcttcat tccatcgtga aaaatatcca
29461 ctcccaatat ttcattctta gtgtaggaaa ctccacagac atgaacttgg aaaatagttt
29521 tcagagaatt acaccttcgg gggacttctt cctcttaagg gttttattga aggcagtgcc
29581 ttttgtgatg aaaaccaaca gcaccaagga ggggagatga atattgtgtg tccctgaagt
29641 gacaccttga gaagaacatg gtattgtctg tgggtacctg gctagggagg cacaacctga
29701 atctaaccat gaagaaacag^ag'aacatgaa atacagaaca tgctgttttt aaaaggacta
29761 tattcttcaa aactgtcaat attataaaat acaaagaaag ggtgtggaaa tgttttagac
29821 taaaagagac cacagagaca tgacaatgtg tgctgagggg gcaaaatgct gaattaacac
29881 ctaattgagc caagttattg ataaaatcag aatgctcatg gtaggttaga aaaaaatatt
29941 gtcattgttc agtttactgg gattgacaat tgtgctatgg tcatgtcaaa atattgtgat
30001 ttttaggaaa tgtacactga aatatttagg gataaagagc catgatgtgt gcaacttact
30061 ctcaaatggc ccaggaaaga aatattattt gttgaggtta ttatcattat tatgattaag
30121 ctgttattat tgattaaatt gttaaattat ttccaaataa aaagcttttt aaaatgtaat
30181 ccttaaagag tgtccctgta aagatacttt taacactgtg atcattgttt aattacagat
30241 gatgggaaga aggaagaatt aaaaatacgg ttttccaagc cctgagaaaa acctctatag
30301 actggatcct tatgaaaatg tcagttctaa atatcacttg acagatgttt ttcccaggct
30361 tttattggga cggacatcag gccaactttc tcccacagac aaggtcttct gtggtggggt
30421 aagtccgctg cagacacatg cctgccaatc atttttaaca caacaatctc cattttcccc
30481 aagttccttc aaataaggac tgaacattta ctgcatttgt gttaacgttt tccctttttc
30541 actgatcggg ttggccaagc cctttttgtc gcttttattt tagttttggg gtacatgtgc
30601 aggtttgata tataggtaaa ttacctgtca tgggggtttg gtgtacagat tatttcatca
30661 cccaggtaat cagcatagta cctgataggg agtttttttt ttttcaccct cctcccaccc
30721 tccaccctca agtaggaccc aggttatgtt gttcccttct ttgtgtccat atgtactcag
30781 tgtttagttc cttcttatac ttgagaacat gcagtatttg attttctgtt cctctgttag
30841 tttgtttagg ataatagctt ccagctccat ccatgttgct gcaaaggtta tgatcttgtt
30901 ctttttatgg ctatgtagta tttcctggtg tacatttacc acgtttcttt atccagtcta
30961 ccatttatgg gcatttaggt tgattccatg tctttgctat tgtgaatagt gctacaatga
31021 acatatgcat acatgtgtct ttatggtaga atgatttata ttcctttagg tgtataccca
31081 ataaggggat ttctgagttg aatggtaatt ctgttttaag ttatttgaga aatcaccaaa
31141 ctagtttcca caatgactga actaatttac attccaacca acagtgtata agagttccct
312Ql ttctccagaa ctttaccggc atctgttatt ttttcacttt ttaatggcag acattttgac
31261 tggtgtgaga tggtatctca ttgttgtttt gatttgcatt tccctaatga ttagtgatat
31321 tgagcatttt ttcatatgct tgttgtccat gtgtatgtct tcttttgaaa agtttctgtt
31381 catgttcttt gcccacttta taatgaggtt gcttgttttt tgcttgtaca tttgttaagg
31441 ttcctaatag attctggcta ttagaccttt gtcagataca tagcttgcaa atattttctc
31501 tcattctata aatagtctcc tttccctgtt gatagtttct tctgggcaat ccctttttga
31561 atatagtcag agagctcatc aattcatgac cttgggagtc ctaagatttt cttctctttc
31621 ccatagtgac ttgactactc actaatatgt ggagtggaag gaagaagaaa ttaccctagt
31681 cccttgtttt tgtagtccta atagatgtgt tcacacagga agagagatat gctggttcag
31741 atgaagtttg ggaatatgga tacaattcca ttcttggagc aattaccaaa cctcaggaga
31801 ttaacacact aaaagtttaa ttatcacact cacaaaggtc agtaatgatt gggtggctct
31861 tctctaagtg atcactcaga aatttaggtt ccttgacttt tgtttgggta attttcactt
31921 tcagtacgaa acctgcagtt gccactgcat gagaagacag agtgggtaat atagggagat
31981 ccacacatag gttcagaagt ggcatacatc agtttagccc acatcataca atgcaacatc
32041 aacactcctc catcaagatt ggagccagcc aggcacagtg gctcatgcct gtaatctcag
32101 ccctttggga ggccaaggca ggcagagtga ttggatccag gatttcaaga ccagtctggg
32161 ggacatggtg aaactccgtc tctacaaaaa atacaaaaat tagacaggca tgttggtgca
32221 tgcctatagt ccccagctac ttgggaagca gagatgggag gattgcttga gcccaggagg 32281 cagaggttac aatgagccga gatcacacca cagcactcca gcctgggtga cagagtaaga 32341 ccctgtctca aaaaacaaac aaacaaacaa aaaaacagtc ttccttcccc tagaacctcg 32401 gtgggccttt acaactgcct caaccagtag aacacggtgg aagggacact gcattacttc 32461 tgaggctagg ttataacagg caatatggct tcaccttgcc tcactctctg tctctgaatg 32521 ctcacccttg gacccaagcc agcagcctgg atcaactggt agacatgtaa gtgaatgagc 32581 ctctggcctt taaaccttcc agaggaagtc ctagatgtga tggagcagaa acaagccatc 32641 ctgctgtgcc ctgttaaatt tctgacctac acaaggataa gaaataatga atcattaaca 32701 ttgatttagg ctaacaagtt ttggaacaac ttattacaca gtaatagaca agtaagacaa 32761 tgggcacctt gctaatctct gcctcaattc tgtagcctct ctgcagagag ttgctcatta 32821 ggattcccag actcccgtct gagagaaaaa tcatcaccac tgctatcacc accacagtta 32881 tcatcagtca ctgcatctgt cagcacctca gcaagaaatg cagatgatac tctgaaaagg 32941 ggaaattgca tagtgctttt gaaaggattt agggaactta ttagtgacac acactaggac 33001 tggcaacaaa ggggggctgt taccaccctg ggccaaatgg gcaagaagtg gtcaccagtt 33061 ttttgcagga gctataacta taggagaggg actagctgaa aagagccaag atttttcaag 33121 tgcaaactga gtcacagcaa agagagacct agggaaataa aaacccagac tctcttttct 33181 ttcaatctct gacttttcgt tgatagtttc tattggctat acccaatagt aaaccaaagg 33241 gcacaggagg ctggttgata cagtccataa ggttcagcct cccagcagac aaaccaggat 33301 gacaaaggtg gagagtagac ctggaggagt aattatgaac attttccaca ttcatcatca 33361 tcaccatcac catcagcatt gtcattatta tcattatcat catcatcacc atcaccatca 33421 ttgtcaccat tatcaatatt atcaccatca ccaacatcat cattgtcacc attatcacta 33481 tcataatcac catcaccatc atcatcatca caatcatcac catcaccatc accagcatta 33541 tcatcaccac tatcatcatc atcttatact acctcattat actacctcat gtgcttacta 33601 cttctgaatt tgcaaaataa tttctcatga tttttctgat atcatcctca gaaggtcttc 33661 cttgctgtat tccctgagtc tagatgagag tttgtcacat aaatggtgct tagttcaatc 33721 ccacaaatat ctttgattgc ctactctatg caaggcttca ggctagaaac atgggacaga 33781 gaataaaatt ccaagatgaa tatgttatgt ctcataagat accaagtgtt aggaaggctt 33841 aatcagaaaa cccttcagag gttgtggtgt tcaaggtccc tttagatgag aggtagatct 33901 caacaggtgt acttaggaga aaatgctctt ccatgcccta gaaaccatat aaaataggtt 33961 taaaagatga ggaaaggctg ggtgcgttga ctcacacctg taatcccagc actttgagag 34021 gccgaggcag gtggaccacc tgaggtcagg agttcaagac cagcctggcc aaaatggcaa 34081 aaccccatct ctactaaaaa tacaaaagtt agctggatgt gttggtgggt gcctgtaatc 34141 ccaggtactt gggaggctga ggcaggagaa tcactggaac tcgggaggtg gaggttacag 34201 tgaactgaga tcgcgccatt gcactccagc ctggatgaca agagtgaaac tccatctcaa 34261 ttaaaaaaaa aaaagatgag gaaataggaa ttatgttctg ggagcagaaa atatttcagt 34321 tttactggaa cacagaactt acagagataa aatgtaagtt atgaaattga agaagcagtg 34381 ggtcatataa gtcttggaag ccaagatgag gcatttatac tcatcacatt aggtacaagg 34441 aagtcattga tagtttttga gcacaggaag gaagtattct aactgttctt tcacaatatc 34501 aggtttacag taatagatag gataaatgtg gtgagcacag acatggtgtg ggggaaatta 34561 tttataggac tactgttcta gtttcagtaa aaagaacctc taaagggttg cgaggggcag 34621 aaatggaata aaaggatgtg gcaacaggct gacagtgtga gatcgaagaa agaaccaaga 34681 aagatggctc tttttacagc cagtcacttc tgggcatgga ggatgccact tgttcaacat 34741 cacaaggatt tatcatgcaa ttgttttgtg ccaggcactg ctcttgacta tgtaaacaca 34801 gcaggaagca agacagaaaa aacacaatct ctctttccac ccaggttcca taagagatgc 34861 acaagcagga aattcaggac taagagattg cacacgggtt ttcctctgcc tgaaatatcc 34921 ttcctcttat ctcctttacc tagaaaacac ctcctcatcc cttaaaacca cccaactccc 34981 caatttaaac atttgtccct gattctttca ttgaactttg ctcattcttc cggcacttcc 35041 cttgagagac aactaggttt ttttgggtaa gaaaaacttt tataatcgta gtagcttgtg 35101 aaaagtctct tggaggttgt gttgaaacta tgagttcaaa ccattagtaa gtcaaaaatc 35161 tgtaaaattt catcagttta tcagggttga agctcattat ttgtcctaag agtttggaaa 35221 catagaggac cctgccaacc atccacctcc tcatgctcac cccagatgga atcccacggg 35281 tatgttatgc agcatgatgc ctgtgcatgc aatctctggg cctgctgggt gatatttgtc 35341 cctgtcattc tgttctcact cactggacta aggcagcagg tgcatgtggg tctggggcac 35401 atggacaatc agcacagccc ttggggtcaa gctgacttga gttgaaattg aggcttttct 35461 acctgagcct caaggccttg gacaaggatc tcaatgctgt ggagcctcag cttttttttt 35521 ttttttaacc tgttaaggag gaagaagaaa gcctgactct cgagttccta ggagaattaa 35581 atagtgtgat gaatacaaag ctcctggccc attgcaggca tttaacaagt ggtgtgcttt 35641 tatgtgggtc agataggatt ttcacaaata gcaagggcat tctggaagtt gaaaaacact 35701 ctgatgacaa caataacctg caggctatcg ggcatatgtg aaaagggaag aaagtggttc 35761 cagtggggaa gagggcagtg gaaggtggag agcctatgac agtgctggca ccagccacca 35821 tggcactata tcctgaaaat ttagttatgg cctaggtaga cttttcatta cttaatgggc 35881 aaaaaatgac aaaatgcgtg ttgaagtttc attcatgcat tgattcaaca aatatttatt 35941 cagtgcctac tatgtgccag gcactattta caacagtgaa taagacagag aaaaccattg 36001 ctctctggaa gatttcatcc tagtgatggg agacaaataa gaagcaaata tagaagcaaa 36061 atatcagagt cgtgagtgct ttgaggagca atgaagcatg ggtgcggtaa gttgagtgga 36121 gccaggactg ggcctcatct ttcagcaggc tcggggaccc ccacgaccct tctgaacctg 36181 aatgttctgg tggacagcag ccaccgcaaa gtctgggccc atcctgcttc tttcccagtc 36241 gtggtttgca gctagccttc gggaatcagt tcccagtcat cacacatact ttccccaaaa
36301 cttgcagctt tattaaagaa aatcttcatg acaggaaaat acgtgtggct aYggaggaKc
36361 acaaataaaa atttaaaata aattaaaaac tgaacaagtg tatttgtccc tggattccag
36421 ggaaaattcc agacRcgccc acagccggtg gcagcattga tcctcagccc aggtggtcaa
36481 caaaagaaga actcaggcac acttccctgg ggcaggagta aaggaacaca ctgtgcacac
36541 ctaacaagaa atgacaggca ggtgtgagac agaaggttcc agaagtagag cagaggcagg
36601 acacaagatg gtccaaggct cagccagagg gaaggttacc tgctgcattt gggtctacag
36661 tccttggtcc tatagctgtc agcagaggtg ctatattagt ccgttttcat gctgctgata
36721 aagacatacc tgagactggg aagaaataaa ggtttaatgg acttacagtt ccatgtgtct
36781 ggggacgcct cacaagcatg gcagaaggtg aacagcacat ctcacatggc agcagacaag
36841 aaaagaaaga gagttagtgc aggggaattc ctcttattaa aaccatcagt tgtcatgaga
36901 cttactcact atcatgagaa cagcacagga aaggcttgcc ccatgattca attgcctccc
36961 atcgagtccc ttccacaaca cgtgggaatt caagattaaa tttgggtggg gacacagcca
37021 aaccatatca ttccgccctg gcccctccca aatcttacat ccttacattt caaaactaat
37081 cataccttcc cagcagtccc caaaagtctt aactcatttc agcattaact tagaaatcca
37141 cagtccaaag tctcatctga gacaaggcaa gtcccttcca cctatgaacc tgtaaaatca
37201 aaagcagatt agttactttc tagatacagt gggggtacag gcattgggta aatacactga
37261 ggatgccatg aggcgtcccc agccacatgg aactataagt ccattaaacc tttatttctt
37321 cttgcagact ctcattacaa atgagataaa ttggccaaaa caaaggggct acaggcccca
37381 tgcaagtctg aaattcagca gggagtcaaa tcttaaagct ccaaaatgat ctcctttgat
37441 ttcgtgtctc acattcaggt cacactgatg caagaggtgg gttctcatgg tcttgggcag
37501 ctccaccctt gtggtttttc aggttacagc cttgctcctg gctgctttca taggctggca
37561 ttgagtgtct gcagcttttc tgggtgcaca gtgcaagctg tgggtggatc taccattttg
37621 gggtctgaag gacagtcgtc ctcttctcac agctccacta ggcagtgccc cagtagatac
37681 tctgtgtgag ggctccaatc ccacatttcc cttctgccct agcagaggtt ctccatgagg
37741 gccccacccc tgcagcaaac ttatccctgg acatccaggc atttccatac atcttctgaa
37801 acctgggtga atgttcccaa acctcaattc ttgatttctg tgcacccaca ggctcaacac
37861 caggtggaag ctgccaaggc ttgaggcttg caccctctga aggcacagcc tgagctctat
37921 gttggcccct ttcagccatg gctggggcag ctgggatgca gggcaccatg ttcctaggct
37981 gcacacagca cggggaccct ggacctggcc cgcaaaacta ctttttcctc ctaggcctct
38041 gggcttgtga tgggaggggc tgctgtgaag acctctgaca tgtcctggag acattttccc
38101 cattgtcttg gggattaaca ttcggtttct cattacttat gcaaatttct gcaggtgact
38161 tgaatttctt ctcagaaaac aggattttct tctctatcac attgtcaggt tgcaaatttt
38221 ctgaacttta atgctctgct tcttttataa aactgaatgc ttttaacagc accaaagtca
38281 catcttgaat gctttgctgc ttagaaattt cttccaccaa tatcttaaat taactttttc
38341 aagttcaaag ttccacaaat ctctagggca gaggcaaaat gccaccagtc tctttgctaa
38401 aacataacaa gagtcacctt tgctccagtt cctaacaagc tccttatctc catatgagac
38461 cacctcagcc tggaccttat tgttcatatc actctcaaca gttttgtcaa agccattcaa
38521 caagtctctt ggaagttcca aactttccca catttttcta tcttcttctg agccctccaa
38581 actgtttcaa tctctgcctg ttacccagtt ctaaagttgc ttccatattt ttgggtaact
38641 tttoagcaac accccactct attggtacca atttactgta ttagtcaggt ttcatgctgc
38701 tgataagaca taactgaaat tgggaagaaa aaaggtttag Yggacttaca tttccatggg
38761 gctggggaca cctcacaatc atggcagaag gtgaaaggca tgtcttacgt ggcagcagac
38821 aggagaagag agctcgtgtg ggagatttcc tctttttaaa accatcagat ctcatgagac
38881 attcattgtc atgagaacag catgggaaag acttgccccc atgattcaat tacctcccac
38941 tgggtctttc ccacaacatg tgggaattca agataagatt tgggtgggga cacagccaaa
39001 ccatatcagg cacctctgat ggcttcttgc agaccctctc tggaggagca ctcaagacca
39061 agaagggcag gagactatca ggaccacttg agaaggctgc tgcttgattt ccccgctcca
39121 tgctagcgcc cttcatccat gctccacaca gaagcatttg cactcaatac tcctcacacc
39181 cttcaccttc cctccccact gtgcctaccc ttacccctca cccactctga agtcaaattc
39241 ctgaggacag cctgtctccc tggcttttcc ttcagcctga cactcagtcc aggagatgct
39301 taaaagctgg gctctgtcat gttgccttag gcaaaccatg caggcaactc acacttcagc
39361 tccctcttct gtaaagtgga aatgctaatt cctctgaggc atttgtgaag aataagtaga
39421 aaactaacat gtaaaggatt cagcatagtg actggcacac agcaagtggt gagcacatgt
39481 ttgctattag ggctgatact ggagctattg aattgcttgg gctccctgca tggtgctgtc
39541 actcacatac ctgggccatg ctcagtgcca cccatcacct ggcaggggct tcatcttcag
39601 aactcaaagg cWagcttcct ggaccctcca gcttcaccta aatgcttccc tttagttatc
39661 tcaggtacca gatcaccctt ctagcatagc accgtccttt tcaaaattga cagtatctgt
39721 ttatatatct aaaccttccc tccataatat aaactccaga aggtgaggaa atgtgtctga
39781 tccatcttgg catctccatt atggagcaaa ttgacttgta ttgagggaca cagagagatg
39841 gacagtcaga gggagaggca aagctaataa aattcccaat ttattttcca ccaatacccc
39901 cctatgcatt gttaataaaa acacacacac acacacgcag aaacacacac acacagaata
39961 aataaaatgt atatatttcc atcttgttcc aagtaaatca ttccatatcc tcagccataa
40021 cattttaata aagataaaat taatttaaaa tagataattt agttgactca tgtgcccaaa
40081 tacatagatt ttgcaagtag ctattgtgac aatagcagct aatatttatt gaaaacgtac
40141 tatgtgccag gaaagatgct aagggcacca tctttttaac taaattggtc atcccaacaa 40201 gctgcaagag gatgttgatg tcctgatttt atactttgtg aaatagatcc agagatgtta
40261 aggaggtgct caaggtcaca cagcatagta cagcacttga aattagtttt ttttttctct
40321 cttttgtgtt atgagttgaa gtggatccct aaaaatgaca taagccccag tggtccaggc
40381 acagtgccag ctttatccag catagtgggc actgagttgg gtttattttc atttaaaaaa
40441 tggacaagaa gcctgggcaa catggtgaaa ccctgtctcc acaaaaaata caaaaattag
40501 cccagcaagg tggcctaggg ctcttgtcct ggctacgcag gaggctaagg tgggagaatc
40561 actttagccc cggagacaga ggttgcagtg agctgaggtt gcgccactgc actccagcct
40621 gggcaataaa gagagaccct atctcaaaaa atgaataaat aaatagttaa aataaaataa
40681 gataagagag gttgaatgct ggtttgaggc cacatacctt agaggactgg ctggttcaca
40741 gacatctttt cactgcatgc caagctaagt caaataagct agagtgacag tagaatccca
40801 atctatgact tctggtgttc taKgtcttgt aacctaagca ggaggttgga agtgggaaga
40861 tgctgaacct tcagggaagg tgttgtcagc gaggaggtcg gcatgggatt tttgtggtga
40921 ggaatgagac taaaacagta gtttgagatc aaatggagga tggtggagaa gtaagtttag
40981 aatttctttt tctttctttc cttttcttct tttttgacag gatcctgatg tgccacctag
41041 gctggagtgc agggacacga tcacagctta ctgtagcctc agcctcctga ggagctggga
41101 ctacagacac ttgccaccat gcttggctat tttttttttt tagacagtgt ctgtccatgt
41161 ttcccaggct gaagtttaga acttcttgct ggggaaacct cttatgcagg tcaatcaagg
41221 tgctgaacat gaacgtttta ggtaaagatg gttaaggaga ccgacgtgac agagcaaata
41281 ttacaaaagg agagctgaca gtacatacgg aaccttagga gatacaaagg ggtgggtgag
41341 ggaaggattc attctttaga gatactgtgt ctgaggggat gatggggcct ccaggtaggg
41401 aggtctggcg agcagctgaa gaggggagga aggcaggaaa tgtggagtgc ctcctacaca
41461 gagctgagga ctgcagagag agcagaggaa gtttgtctgc tcaagatgga gaagtcaaga
41521 gaaagaagag catggggctt aatctgggcc cagagctgct ccccatgcat ggatggaagg
41581 aggctgggca gttcattaag gatgtgactg cagcttcaga agaaaaccag caaccgcagc
41641 atcatggaag tgtgggggca agagagcttc aaggagaggc tgatcaacca tatccactac
41701 tgccacagtc acagataatg tcactagaga gatggccact Rggtttgtca ttcagaggtc
41761 aacctttttc agtgcctttt ggcaaacaaa atggtagaga tttgagaaag gactctggat
41821 agtatcaagg gagtagaagc atcctgtaca gaggacttct tcaaccaggt cagcagtcac
41881 caagaggagg gagattgagg agagtctaga ggaagagcat gtggccccaa tggatcggaa
41941 gatggccact ccagactcca gtctatctct aacattaaac tcctgagaga tttggggcaa
42001 atatcttcat gaaggtgagc ctctattttc tcatttgttt attttggaaa aattaaagag
42061 aatagtatat cacatcacac agttcccaca ataccagccc atgccaatcc tgattcatca
42121 ctactcaaca cgctttgtcc tcccactggt ttatttgcaa gttcattcga gatatgaaat
42181 catttcttcc ttaaataatc ttttgaattt ttctccaaca gatacagtca tttaaaagta
42241 taatttggga ggctgaggtg ggtggatcac gaggtcagga gattgagacc accctggcca
42301 acatggtgaa accccatctc tattaaaaat acaaaaatta gctgggtgtg ctggtgtgtg
42361 cctgtaatcc cagccacttg ggaggctgag gcaggagaat agcttgaacc cgggaggcag
42421 agattgcagt gagcagagat catgccactg cactccagcc tggtgacaga atgagactcc
42481 atctcaaaaa aaaaaaataa aagtatagcc acatagcRtt actacatcta aaaagttaac
42541 aggaatttct gaatagcacc taagtgttca aagttccccc tttttctcac actatttgag
42601 ggtaaggcag agttgcttta ttaaaatcaa gatccaaagg tctacacatg atccacacat
42661 gacctttggc taacatgtgt ttctctcttt tacatctgta aattttctct ctctgtgtct
42721 ttcttcttga tgcttatttg ttgaagaaac aaggtaatat gtcctgtaga gtttcccacc
42781 atgtggatct cactggtgcc atctacatgg tgccatttaa taggttcctc tgttccctgt
42841 atgttctgtc agctggaagt tagacctgag gattgctgca gtccatattt gatttttgtc
42901 gaaaaatgtt gtgtattttc taatgcatca cgtatgtaca agtttctcat ggtctctttt
42961 ttttgtgatg ttcagatgga tgtgtgggtt cagagaccat ctactcaatc aatccacagt
43021 aaattcccaa tacttttact atgttgtcaa gaaactttaa gttcttacta tcttacaaac
43081 catctaatcc attttgacta gcagaaaatt caagactcta cattaagtag atcaagacat
43141 acaaaatacc aatgtcttaa atataaaaat tatatcattt ccatgatgaa ctatggtcaa
43201 tttttcaaaa atggaagcaa tgttcagaat gtggtctgtc aatgaaatgg aaataataag
43261 tatggaatat ctcaaacaaR caaacaaaca aaaaaacgga tattcagcca ggcatggcgg
43321 caagtgcctg tagtcccagc tattcaggag gcccaagctg gaggatggct tgagtctcag
43381 aattggaggc tgcagtgagc tatgatcaca tgactgtact tcagcctggg tgacagagag
43441 aaatgctatc tctggaaaaa aaaaaagtat gaatgattgt tgtgggggga gcagcttgca
43501 caaactatcc gtctaggagg atattagcag atccctgtag acgcaaacta tttctaacaa
43561 cacaggaggt gctagaagct gatgctggag gttactgaca gtaactaatg tggagatttt
43621 ccttatcata aagggcctgc actagatgct ggcagagaga atggaaaact aggtgtattt
43681 tttttagata ttccatacct attatttcca tttcattgac agaccacatt ctgaacattg
43741 ctttcatttt tgaaaaattg aaggaagaac tgaaaggatt tggtaaataa ttttaaaaag
43801 agagagtgga gatatcacca ataccttgac caactaactc ttaagtaaca aaagaatgtg
43861 aattgggagt ttcataaaaa tatgaaaaat atcctgcaga tttaggccaa cggacacccc
43921 accttgtatt cctctacctt gtggataact atcatggtct gttatataac cccagaaatt
43981 tccctaagcc atcagaatcc acccctcatc tctaaatatt ttttataaca ctgctcataa
44041 ataagaagta gagccattgc ttggaggtag tctataaagc agaggcatgg ggcaaatggc
44101 attttgaagc aaaacaaaaa gttttcaatt tatcaggaaa gaaaaaaaaa acaaatcaga 44161 ccctgcataa caaattggtt atataaatac tctgcggttg ggagagctgc caatacccct
44221 gcacatacag ataattttgt aggagaaagt aaaaattttg atcaacacca gcagagccta
44281 ataggctgga agcctgaggc ctgacaaaga ggcaggaggc acaccagaaa acccctctaa
44341 ccttggaggt gagaaagaag gcaaagggag ggaggaggcg aaacctagaa ataaagatct
44401 gggagaaaca acccaatctt ggagcgagcc ccaaaaatgc ttgttagaag acccacattg
44461 aacaaaagat ctaaatctca tcattttggt ctaagatttc aatggagaat ttccccaccc
44521 tcacccagtc cctgccaacc tgttgttacc catccagcag gacaaggggc ttaccgctgc
44581 cagctgcctc tctaagagga atttcaacca caaatgctgg catttgacca gagacggccc
44641 acatgggcat gcagaattag gtcttcattg gaataactga gcagaagagc tggggcctgg
44701 aaagagactt cctggacatc ccagggccct tgtagtggcc aggccactgt cttgagacct
44761 caggttctgt ccaggagggt actgtggtct ggtgtggaag gggtcaatat ttcctataaa
44821 gaaggagtgt tttaagggtg ataagaactc agccatctct tccctattta agaggaagtc
44881 ggtgtgagtc cttgggccca gagttacatg gaaaaagata tcggttcttg taagaaagta
44341 ggctttgcac cacagaagga ggcatttgca atgcagagac cggaaaggag aaaagagggt
45001 gacgtgtgct ccatagagag cagcagggga gcagatcagg atccccaccc cttctcctga
45061 gagtactcga gcctcacttg agacaaattc ttaagataat cataaaaatc catagcaact
45121 ttgttgatgt tggggtttgc actgagattc caaatcaaga ggctgggcaa gcgaaacagg
45181 ttcggatttc tttcacacag caggtgaaag aatgtaagaa ctcctggtca aagtcaagag
45241 cccggcactg cctgctctgg tcacatctat tctgccccac atttctataa caggaaatta
45301 ggccaaaatg catctccagc atcattcact tccaaaatgc tctccataac aaactttttc
45361 acaatggtct gtgaaattgt ttgttctgtt tatcctccga tggaggagag aaactgagtc
45421 caataRatga gttggcattt ccaagccttt cacccatgtc tggggccatg aggatgaaaa
45481 tgcacccaca cctcaagtaa atgctgtatc agtgtttcag cacaaaaaag aaYaagatgc
45541 ttttgagagt gtgtggcaag tgatctgagt catgaggtct ccagaaccct gatttgaaca
45601 gtaaaatgaa ggaaatagcc aaaggcctct cctatgctgc tcctccggta ttgactgcct
45661 ggacagcact acagccttgg ggagacagat gctcacaaga caattctctg ctggaaaccg
45721 gaaggctgtg tgtgtgtgtg tgtgtgtgtg tgcgtgtgca gcctcagaca ggtgacctcg
45781 gaaagcagga gtgaggcctt ggggaagttg aggctggcaa gaaggaaaag tcaactctgg
45841 gaagcatcat taggctggtc atggccacaa gcagcagggg ctccagccag ccagcaccct
45901 gtgaggagcc aagtggaatg ccggccagaa gcatccccag gggagcacag gtgagggaag
45961 cacgtacccc acccccaggg cttggggctt gccctgagcg ttgactttgt gtcactctgg
46021 cctgcacaca cacaggccag gcacatggtg cctcccaaga gcagaaatca aaattctatg
46081 tcaatgccaa gtgcttcttg gccgagggta ccgttcccca tggccacagc agggatgcca
46141 ggtgagacta gaggaaccta agcctggggg gtccccaaag tcgggtcccc aaagtcgggt
46201 ccccaaagtc atcacccaac aaatcttttc tttcttctta aaaacacatg tactcatcat
46261 tctggaagat gccctgacat tccaagtttg gcctgcacac tctgttctca caccggccct
46321 gccatcccta ctacgctcca tcctctttgc tccttcttgt ctagcagaag ggagcaggag
46381 cctgcctgcc agctgtgtct cctaaggcag ccctggctgg gcttctctcc cctctgcctg
46441 taaggcccaa tgttcagatg tcaagggtct gtctttttat agaaacaaag agccatgcag
46501 aatcactgtt tttgggattg ctgtccttgc agctgtgatg aaagtcgatg actttccaat
46561 catttcctct aacttctgaa gggctggagt agcaaatgcc ccttcccctc ttttgcatgt
46621 ttgcaggatg ggagtttcag atgttgttta cctgccagac gtggagtgga ggcttcccaa
46681 agcagcatga tgtacccact tcggggccac atggtaacgg ggactgagag aacagggcat
46741 cttttctaag acggaccgag ccaatctgag cagtctggcc ctgcctgcaa agtctgtgtg
46801 gccttaggaa cgtaatttaa cctccatggg ctccatttgt ttctgtttga gaccctcttc
46861 ctaattgtgc ccgtggtgcc tagttcataa tcagttctca gttcatggtg gccccccttc
46921 atcccccaaa tgtcaaagtc tccagcttcc catttccctt ccattcctga ctctaagtcc
46981 ttaaattggg tgaccattaa tattactgct aggagcattt tcagagcact ttacatttac
47041 agcgtttttt ccttcccaaa ttgttctcgg tcctttcttc ggaataggta ttaaaaacac
47101 ctcattggac aaattccttt ccttagaaga tacatcatga atatggttgg agaaggcagg
47161 atcataattc ggagaattca gggttgattt cccttacctt cctgctcttt tgctgtaaag
47221 tccggagccc caggaagtgc cgccaaacta ccccttattt tccctccaag agcagggcag
47281 aataaattgc tattttgttt ggttttgttt ctcatataca aggcctccca ggccctggaa
47341 cctaggctac tggcatttta attaatgtta atgactggga tgacctcctc cccattgaat
47401 ctgagtaagg gaaataaaca ggcaaacccg aacaaagcca tgtgcggatg tggtaccctc
47461 tagaaaagct gacctctccg atggctggcc agggtctcta accaggccca tactccttcc
47521 tcatataccc ccaattttga atttttttag ttcaaataga aattggttgc agggggaagc
47581 aggtagattt cYtcctttaa tacataaatg attctgggtc ctcggaatca gaatccccac
47641 tttcttatta ctaggagcat ttttactctg agatcaatac aaccagaatg tgtccaacca
47701 taactttaca acaacagaaa agatacatag caaatgcctt caaatacctg gaatctggcc
47761 ttgaaatcga cctggcattt aaatcgacat gtatatccca gttgggtcaa aacatagtca
47821 aggtttcccc cagagatact ctgtttcatt ttccttttca agtaaatttc tatttttccc
47881 cccaaccttt acagcttaag cgttttatcc agtttaaacg ttagggattt aaattgctat
47941 tttagtggaa gaggcttgat taaaacctgt gtcctcatca aattattgga cagtggttct
48001 tgggctctgt ttacattagt gtcaacctta atttaaattc cgcataacta tatttgcttc
48061 gctaaatgca ttcaccagga acacaccaca aattactttg cattatgagc cgagtctctt 48121 tgtctattgc aaagtgtttt gaaattagca ccataagtaa ctagctgtaa gtgtcactcc
48181 atctccttaa tgcctcatta atcaaactcc atggcagctg atgtgatgtc caagccccta
48241 acatataaga tcagtatcat ttactccttg catttgacca cggtctttaa acttgatttg
48301 ttatttgtgc atttgccctc gctggagctc tgctcctcGc tcagtggcct cccaacacgt
48361 ggggctttgt atggatacca gttgcatgtg tactctttgc agaaattgga aataaaaacc
48421 catttcccat gttgtggaaa tgcatagcct caaagtcagg aaattctttt aatggaagaa
48481 gagttccact tattcccctg agttatccac agtgMttccc catgattttc agattaatgc
48541 taaaatgcca taatggaaaa atttccctgg gcaagttttt ctctatgtca agaattagag
48601 aaaggaatta cgttcaccca ggcagactac aatttccatc ttggcctcag ttccccaaaa
48661 aaccagatac atttagtcta agcacttaat caggaaaaaa aaatgtattt ttcccttatt
48721 atgtgatacg aattattctg tactaaatac aaaattttag aatttatgct tgcaaagaaa
48781 tacagaacaa tttatactta ataattttat tacatgtaca cacaaaatca tagcaaaata
48841 tgatatattt tataggattt ttttgtcttt tcatgatgcc atcgaatatt tgcaaatgcc
48901 taaatggaac cctttcttca ttctccacac aagtccaaaa acacaaacac acacgcacac
48961 acatacacct atacacatgc atccttttaa ccaaaggtaa tactcactgg tgttaaaact
49021 aacagctcta ctggacaatg gcttcatgga cattttagag gcagtcttaa gtctgStgtg
49081 ggcacaggca tgtcggatat gatttccctg cagggatgcc ctctcggtgg ctgtggggga
49141 tgcttgcggc ccctcgcctg ccagcgctga gctcagcctc acagtgagcc gagatgaaca
49201 ggtgctctct tctctggcgg cggcggcacg tgaggtcggc tccttgcaga acagcgtctc
49261 agtgggagac ctttagcttc acggcctttc cttctgacca cccagcagga aaaatatacg
49321 aaagcgtttt tttctgccag ttttgtcgat gagacaaaat acaaaaacaa aaaaacacac
49381 agcattaaaa ctttgtattt cccacaggtg ggcttYggaa atgtttacca cagtcatagt
49441 aaaacactag ttttcttcgg aattaaattt tttgcctttc caccttataa aaccattcag
49501 gctaaataac cacattacaa tagaattaaa cattgaagcc tggctcccga aatcaacatc
49561 agtgggaaaa cccactgatt cggaacacac acccaacagt gcaaatctat gttagatttt
49621 gaMtttaata tcaacttaat gtgactgtag tttatattat tatcacatct actttctttt
49681 atatttaaaa taggctctga ttttagaaga gcaaatgtaa aaaatatttt gattatttgt
49741 tgatgacatt tactctgatt ataggtgtaa tctatggcaa ttccaaaagt accttgtatg
49801 tcccatctcc agttcatcct gcttttaccc tgagtccagc aacctctatt cattaggatt
49861 ctgaacaaaa taagcatgaa aggggaaatc caaaataata ttcagaagtg gcaaatcata
49921 actgaacata gttttaaact tgacaattta aaacttttgc ctcttttcag cagccagaag
49981 aagtctggtt attttaagtc aaaatctctg ccccaaaacc tcctttccct gcactccatg
50041 aacacctctc tcccttttcc ccaaaggcaa cattttggct tggcaagcaa tacacatcaa
50101 tgaacccttc catttaaatg Ycccacaaag attgcatccg atcacagctt gcaagaaaRa
50161 aagaaaggaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agagaaatca
50221 gacataggca gtgtcaaatg tcttatacgt ccgccccctc tgttcttcca ttctctccct
50281 caccatcctc ttgtaaaata aaagagagcc gagcataact tgttacagta aaggcacccc
50341 gagatttagg gggataagta cggcctactt gttatctgca atgctgcagc tgtgctttta
50401 tctggccaaa ggagacaggg gacaacaaaa gatcccaaag aagggacagt ggacgcatgg
50461 gaaaaccact accccagcat tgtggtgccc ttctgctgag caaatagttc aaactgttca
50521 ttcccatctt ctattctctc cctggtacat tgtttgtgcc cttgcatttc attctgcaag
50581 gaaggttgct ctctgggcct ataagcaaac agtccagaaa agggctagcc atcctcttct
50641 cttccccgga gaactagcat ttttatttcg gctccaagct gtcaaagatc tggagagttt
50701 atgtcagtgt cctgaattaa ttgctttaag ctggagcaag ctccacgggc taccaaatgt
50761 ccaaaagatg tttattaata acatttcctc caggatcatg gctccaagct gtctctgggt
50821 ggtggctttt ctatgctgcc accctctcac ccctggcctc ttaatgctca ggaggggatt
50881 gcctctttca gtcatcatga caaaatttag aattgcccgg gatgaaataa atatccacct
50941 atgtcacagg gtgtgcacgg cggatatctt tgtcctgact cagaaagagt tatagaagcc
51001 ctgtgtgcaa cctacaagtg gtctggggga gagcaaaaag cagccaggac ccccctctgt
51061 gaaagctcat ttagccaata caattagcaa aaggagcagg agaaatcggg gctttggctt
51121 cccctcccta cctcctcctc caaaagaaaa ttcagtgagg ctgacaacac aatgcatttt
51181 gaagttttcc tggtaccaaa aaaaagcctc tttaatagaa tgaaataata aaccaattta
51241 tatacYgata taagactagg atcgtgaatg ccattgtgta attttccact tgacttttcR
51301 ttatctacct gaacaaaatc aatctatatg tatgggatca acatacaatt ccagagataa
51361 aagctgaaaa taaatgcgct ttgcatatcc tggagataaa agacacaatc cttggtactg
51421 ccttttcttc tccagcagat gcatttataa aataaattgt tgtgtcaagg aaaaagtgag
51481 acgtagcagt gtatcttgag tgtggactcc tttgctggaa atattgatcc tgcaaatgaa
51541 cagtggttaa taagtaccct gcagacgata tcaggtcttt tgccctagtt tacccagagt
51601 gcagtctggg tcaatgtttc tcccaaccaa agctcctcac ctccaaaaaa aaaaaaaaaa
51661 gacttcccct attagcatgg atttaaagct gaggacttca agggcttttt aaagccactc
51721 tacagagaca aagcacattg aaaattcaca ttacattaga aaaccccatg cagcaaacgc
51781 ccagcttgtg aagcacacag tcctcaaaag ggaaggagag atggttccat gcactttagg
51841 gttgacattt attactttaa acaggttggg ttccattcct tgtatgcctt tctttgctcc
51901 aataattagc aatggtgcta aaagtagtaa agctctctcc ccattagcca tgccatgggt
51961 cctccttaat tccctttttt tggaatgtgt cactgaatct gcgtgaatct gtgtgccctg
52021 gtgggcagat ggtggggcat tgttgagggt gctgtggctg tttgtgtgcc ctgttgtgtt 52081 cagggctctt cgaggaaagt acacccactc tgccYtccca gggtgggctc accttggttg 52141 ctgttcactt tcctgtaagg gaggggttca tttccaacgt tgtatagata gcaaagagag 52201 gcccacaagg catgggacca tctttcaccc caaacaatca ctctgagttt gtgaatattt 52261 tccggtcata gaaagagatt tccaaaaatt agctactctt cattgtttat tcattgttag 52321 attcagtaac ctcaggggag cttaaataYc taccatggca gcgtgattgt cacagcttca 52381 gctcttaggg caggacaact ccacacttgt atattttagg atctttgcat tgacaaatgt 52441 taatggctgt tggtctattg gttcagcaag attttgcaat tcctgatgtt cacagacctc 52501 cttcccttct cctgggctct gtattcacac acagcaccca tttggactgg gaccattgcc 52561 tcttgattag caaatctggt gctgtgctcc actcctcagt ttatatttgt tgaaaactct 52621 gaggaaagtg tccaggagca atctgatgca gtgtcatggc ctttggggaa atgcttggga 52681 gggtcaaagc tcagcctggg gagaagctca tgtgctttta gtcacagaag actcttagga 52741 caatcagtgt gctttattca tttcatcttt ttgttccagt agctgtaaat aagcaagtct 52801 agagaaagtg taagatgtgg tcaagaagag ttatgcagtc attagacttt tgtttgaaaa 52861 gagaacacct tcctgaaggc atgttttgaa aaggtcctat attttcatga cagaaatgat 52921 gccttgaatg gctacagctt tcagccttgg tgagccccag ccttatttga tccatatttt 52981 aattactctg tgattcaaat ctgcatcgta gggaatcagt acagcgacat aagagcttca 53041 accagcataa agaacatttt caggagcttt gtacccctta agttccctga ggaaagacaa 53101 cacaccagaa caggtgtcac tatttccaca gagaaacctg ctcatgctgg aaaatgtctt 53161 gctaagtggc ctcagaacca tcttctcagg ctggaacagt gctccaccca catgcagaat 53221 cctgacacag tggaacgcag cccatgttga tgaaataaat atttagtaaa caggagaaac 53281 tgagtttttc aaaaaggtat tattataatg tttttagtga tcatgtctta gttgaaccat 53341 cttgatgcca tttcctagag gaagaaaaat gtacagtaag aagtccagta aatatatgta 53401 gatgcacatg tttcaataaa tacaatcaga tggaaagttg tcaacttttg ctagctctag 53461 cattttaaaa actgtacatc aacagttcaa atctttaaaa aaaatagatg aagggtataa 53521 aacaacttaa agtccaatgc gtcccttcac accaggcttg atpgagaaat gagaaaccca 53581 ttttacaggt gaactaaaat gagataggta tttcatttac ccttttacaa aggccaaatg 53641 ggaatgcttt ccaaagttgt ttttatattg gaggtaaaga gggggaacag gaattgggtg 53701 aaacatagta ctcaatcaga atctcctgca agtcttctag aagcattcac tggaactcag 53761 tgacaataaa atccctcccc atggtgttcc agattaccaa cacagatatc cattcagaag 53821 aggatgagat ctcagaatgg ggccagaaga ggactttgct ctaccctaaa gacaMcgaga 53881 tctccacagt acttaacgtg tccttcaagt gctccctgat caagatagtt gttttttaaa 53941 atgaaaagaa acatgacttg atgacaactt gaacagtgaa ttaaaatttc attttgaaag 54001 tatactgtca ctgtgaaaaa attcctggat tataacacta cagaatgcta tgagagacgt 54061 ttatcattta ttctgtctta cagtctatct caaattgtta taaaatgttt taaatgcaga 54121 aaactaagtg aaaaatctct aaacctaaat tatcatttga ctcataaagc attgctttta 54181 tcacagggga catgcaagaa ggtggctggc cactcctctc atacatatcc ctgtttttct 54241 tgacttgaaa atgtctcaag tatatttatt aaatatgtgt tcattattta acaatgagaa 54301 ctatgtatct tttctgcgtt tttcctacca tgtcacagaa ctcaaaaagc acatgatacc 54361 agggagggaa acaggttcgt tttagagaaa tatgaaaaca gttgttctgt tacataactg 54421 gggtaataac agtattatgg caattccctt taaaatagca tttgatacac ttcaacagag 54481 ggagtgtcag gcattctgtt ttgtacaact tccggctgca Ycagcaccag ttgtgtcaaa 54541 catgttgata taacatggtg tttataaaag tcgagttagc tgttcaaatg tactttattt 54601 ttcttttcca gaaattctac aagacagaga tgtggattca cagtcaaaca cagaaaggat 54661 acttagttct caatgaagcc ggggggaggg ggtagtttat gagagcttcc ttctaaaagg 54721 agctgtctgt tccctgaaga aatgacaatt ccttgtccca agcatcactc aaagttcaaa 54781 aRaagcatct gcagcccagc acaagaaagt gttttgctac caacatcctg atgccacttg 54841 caccttaaaa gtggttaaWa cctgctgtgg attgactcag ataaaccagg aacagcatcc 54901 cagaatctct gcacactgac ctgtgaattg atgaagccaa agaccaYtgg tgaagtccat 54961 ttctctccct ttgctttcct ccaagacaaa gaagagtagg aaacacactg ggagtttttt 55021 gttgttgttt tattttgttt tgtcttttct ttgaaaaaaa aaaaaaagat aagcatccat 55081 ttccaagtaa tttgaagcga gataattcta gttagtgaag aatttcaata acggctttcc 55141 tagaaactca agactgattc aaacagcaaa tactgagatg cggttccatc ccaatttatg 55201 gggttttctt ttcctttttg aggaaataaa taattttaaa gagccacaaa agaagggagg 55261 caaattatta gatattttgc ttgacatcaa agtgatttta catttataaa ggcatactgg 55321 ctttcaaaat ttttaatgtt ccagtcctag ctaaaaagtc atgtcatgtS ccccaaaacg 55381 agcctgaatt gatgcgaggt tttatgcaga ctttaggtct ggtgaaggga attttgtgga 55441 gctatgtgtc acggtttggg gtgaaattaa accctacgct tgattccagt gtccattttg 55501 agctcgttag gattgcatgt gtttttcctg tttgcacccg ctttcttcag ccttctatgc 55561 acatacagat ggttacattc tcatactggg tacctgcaga aggtcacatg ctttgcatca 55621 atggcaatta gttccctttt taaaatggaa aatgtcatta agttcttgaa taattcaaga 55681 gtagaggtca caatacagtg tcccttttgg gggtctttat ggtgcaaatg gcaaaacaag 55741 gcgatcctat cagtaagagt agatctaagg tttacaatgt agctctattt agtgtgacag 55801 gaaaattaaa ttacaggagg cactcactca ttgtcacccg ttaacaggct tgggaaaatt 55861 gtgaatccct gggggcaaat caYtgacgac gtgYgagggg aataaaaata tttcatttta 55921 attggctgtt gtttcctttg cttgattttg aaaacaacaa acataaacat gtcccccaca 55981 gttctccttg gaaatagggt cccgagtctc ccaaacacga ccccaactta cagtctctgc 56041 ccagaggccg gcgggttaac cgccctggga agcctaggtc tgttttctca gcgatggttg 56101 agctggtctt tatccagggR ttcctgggaa ggaaaatata gggacaggtt tcaaataaaa 56161 atgggcttcc actactccgc cccccgcccc catcggagtc agctggaatg cagttattat 56221 gtttctggaa caagggcagc ccttcgattc tatcaggact gaggaggagg aagggaaaca 56281 gagtgacgcg gaggccaScc attctttctc ctccccagag gaaaaggagt tcgagctcct 56341 tttgagatac cgggtcggag aaggtgcagc tcgggctgga gcagggtgga cgagtgttcg 56401 aagaggagaa agtgcaggct gggggagcga aacgaaaatc ctgaggtcac taaatgagat 56461 aaaagtgtct ccagggccgt gccagcacat cctagccacc actggtgtca ccagcatccc 56521 gccccccatt ctagccccct ctgttgtccc ccattaagac tggtgggcgt ggctgcctgg 56581 gtcccgctcc caccgcagcg gggtcgcgct cccagtcttg acccaaggcc gtctgcctgt 56641 tcgcccggcc ctcctctgcg cggtgcccag cgggctcatc cggacggggc cgaccttggc 56701 acctgcagga gaaaccctgg actcgccagt gttaccggag acgggagagc gatggggagg 56761 gcgggagagg aatacagctg acagagccca tggcgactgg ggtgggatag gtcgggtccg 56821 Rtgtgaactg gctgcctagg gtcggttccc tgcgctttct agaatttgtg ggggaaaagc 56881 aagaatgacc gtccctctgc ctcgtgctct tttcgatctc tcccccgacc cccagtgaca 56941 tttagcaggc attttccttt cgtggcctYc tccactaaga cccttggaag tcctatgtca 57001 gcgaagtgag cgttgggagg ggacgagaag aaccccccgg agggccaacc cgggttccgc 57061 gtttggcgca tctcggcagc cggagctcgc caaaccggga cagccccacg gacccgcggg 57121 gactgggggc tcggccggct gctctgacga cctcggcgcg cccgatcggc cccggctgcc 57181 tggccccggg tacccgggca cacgccgcct tcccggagcc cgagcacccg ctccggctct 57241 cgcagcctga gcccaaggaa ggctgctctg caggtccccg ggccactcgc ggggagcccg 57301 agccagcccg ccagccagcg gctagggcct cacccaggaa tgaggaaagg gagtgctgct 57361 ttatttcgga agcaggtccg actctgtgaa cacctagata gagggaggca tttaattttt 57421 atatgcacat atatatatat tgcaacgtga tttgtcctag gtagagttat ttgtgcctca 57481 actttgatac atttcatggt agaattaaag gacaaggttc gtgcctgggg tgggggcgga 57541 agagaatagt gaaggggcag tttagatgtt ttttcttttc taaatgattc aatttaagca 57601 aacacttggg ggctccaggc cgctgtaatt ccaaagacaa ttttctcttg ttttaatttg 57661 atttgcattt tttcaacgtt gataatactc aatttcatgg ctgaatacac atgtaaacac 57721 ggttaggtcg tgtttttcgc cacttgcacc aaacagaatg tcgcttgtct cggtgccatg 57781 ggaggttaca cgccgcgtcc tcaaaatatc tgcaagtttc tcctaaatta tttttcaaaa 57841 cttggcctgg aaatttgtct gagcttcaga ggtgacgaac taaaatatat ctaaactgaa 57901 tatatctact gaactagcca aggtgtggga agacctgcac agacattgca aaaaggaagg 57961 cagtaaagga aacaaattcc tatttgatat agaatcaatc ctatttttac ccggcattat 58021 ttttcYatat tgtattaata gttttaaaca atccaaaaat aacaaagaaa ttatcttctc 58081 ccagaaatgt ttttaatgtg gatttcattg ctagaaagac aagcattgag aaatatatag 58141 ctataccttt ataaggagag gtttaattac cactattgag ctattttatc aaacataaaa 58201 attagaagaa aatgcaaggt gaaattgaga aataagcgtt agtcatatca aaactgatat 58261 tcaacaaaat agcactgcca atgacaatga aagtaggatg gtgtgacctg cctgtgagca 58321 gctaccacaa aactgtaatg caagcatatc acacattgtt ttgccctcag gacacaaact 58381 agacagacat tgtgcaaaga aatgtttggc acctaataaa tttattaaaa ctccaaatac 58441 attttatact ccatttacga aactgtgctg gaaaaagtga ggacacactt ttttggtttg 58501 tccaagaata ccttatgtaa taaaatggct gtctaatttc cagagctacc cttcatttag 58561 aaatatatat ttatctgctt gctattgact aatgtggaat attcaatact gtgagcaata 58621 tttctatctc aaatcttaag aatttacaaa ctacaatgtt tgttttattt taaaaatcct 58681 taaaaatgaa aagRatccgt gaaaattcta aatcctaaaa aataaaatag atctgtgaga 58741 cttcttaaaa tgctttatgc tccaggctgg ctgataatag gtaactgtat tttaaattga 58801 ggtgatctaa tttgaaaaat atcttttatc acgtgaaagg catttaggca aacaagaaaa 58861 aattccattt gaaagacaac agactttgaa agagaaagtc gcttaatttt tttaaaaaac 58921 acactcgtgg caggccgact gtccaatgtc tgcaagaaaa ctgttcttca ccatttggct 58981 tttaaaaaaa gaattattaa attttaaatt ataattgtta ggatttgtac taaggttttc 59041 tattgttttg gaagactggc cattatgcct actttatttt acacaaatag atggcagagt 59101 acgcgcgctc ctcacaattg cttcacagca aacccattct tagagatgga gctaatgaag 59161 ggggctaatt gtaacacaca gtctattcag agaatgcaaa tgttggacaa tagagtacat 59221 ctttcccttt ttctattagt aattgaggag taaatggaga tcttgttaag gatcttagaa 59281 gtttgatcgt cactgagtga gtttgccatt ttaatttgct ttcatcccca aaacagatct 59341 gtattgagac atggctacct cagccagaag cagagctgct gcacctcaga cagaatctgt 59401 cctccaccct caacttcatt gaactgggtc tgagctgcag caggaaacag ggagaagggc 59461 tggggtgggg gaagggaggc aagggaggaa tggtcgggaa agtctctgga aagcattcta 59521 gacaggtggg gacttccaga tgtggtatgg ttgggggctt gggaacttgg gagggtgtgc 59581 accttgggag agtcatgagt cctaggaaga agtcacagaa cccaccccaa cccctgcaaa 59641 acgtccagca gctgggagtg gggaggcgct tagctaaggg tgtcatacag ggatagtgat 59701 ctgcctgttc tttcatgatg accaagagcc cagaggcctg agcctgaggt agggaagggg 59761 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtcat gtggtgtgtg agagagagga 59821 ggcagggaag gaggagagag attgtggaat gcaagggaga aatcatgcag ttacttttag 59881 ttgtttactg agcaagggca aaatctaaac cgcgcttgcc acaatcaatg aaaggatcgc 59941 ctcagcttcc agagttgagc tccctaaatg agtctggcat tctgataact gcaggtgagc 60001 acctgagatc aactccttcc cactgctcga tgctgggaag agatagggat gcatttgttc 60061 ctgagctctg ggaagggaga ggcaagaggg ggatttaatt gtctgcctgg gtgaatcgaa 60121 atgtccttta aagcacagaa atcaagagag attggccttt aattggtaaa ctttgctgat 60181 tgggggaggt ggtaattaaa atggaaagag gacaaaatac agattggcta ctgcttcccc 60241 cactgtctcg cccccataaa cagcccccaa aattggtatt ctcctcccct ctggccatct 60301 ttacaaggct cagcacacaa tctgattgtg ggtaattgtg tcattcttgt gtcccaacct 60361 tagtctaaca ctcagttgtc cagtttggag tgagaataca attttatcta gaactctgtt 60421 cacttccaag agttggccat acacatgcca acacgtgagg agctggcctt agaatccaat 60481 tgtctgcctg tctctgaaat gccgaaatgg aagatgctaa gtctgcaggg aatctcacac 60541 cactaaaaat ggacaggcca gggaacttag cttccYggat attgaaaagt aaaggagacc 60601 tccaagccaa gcctgagccc tgatttgagg aggtctagtg ctcatttcca ctctgtgtcc 60661 ccggagcctg gcacaagctt ggcaactgtt cacatgcaca ctcagatgca ctctgctgga 60721 gtggtgctct tgtatgcgaY gggcctactg ggtcctgggc agcatcctcc ctccctgagg 60781 gctgcaggtt gatgtgccta ggccagcatc ctattcaggg gcaaacccca gaggcaggtg 60841 tttatggaga tctgcattgg catatatgtg aggggtggga gggggaggct tgccgYgggg 60901 gctggggcca gggcgtgctg gggaccagct gtgggcggtg tatgcattcc agctatgctg 60961 tcacctgggt tgtcaggcca ccaggtgcag gtcaggggga ggtcagaggc aagagcctct 61021 taggacattc ctagcccttg ctcccagaat ccctcggtag ggaataaggc agacaggctg 61081 tagctcagaa atgatttggc tatgtgggtt gttttaaact tatatgtcag cccttagtct 61141 ctgcccctga cattttctct cttgtctggc attttctcct tctaatcaca atgtaagagc 61201 ctgcttgctg cctcctactt tccagcactg gctagctggc cctggaagca tcagtgggga 61261 gaaaagcgtg gtccaggact gaatgaactt ctgcccaact tcgagggacc atggccacat 61321 aagaattctg actgttaccc cagaagacag catgagaatc cagcctgatc ccagacatca 61381 cccatcctcg tctcttctag gacagtccac ggggtttgtg ccatccttcc aggggg'tacc 61441 aaagcctggc tcttctgaga tcttccagtt tctttccagc ctccccctaa cacttcagat 61501 gtccctactc attttccaga ttctggagtt ctggtgaagg ccggcctcct aattcattga 61561 aataggaatt gtctgtgccc cagagtcctg ggaagccaga ataagttgag ggaatatgag 61621 ttgaaagact ccagctcaaa gctgcgctgt gaaattggag caagttacct aacttctctc 61681 agccccaaga ccattacctg taaattatga tttccaccag caggaagtgg ggaagcttaa 61741 aagagatcct gttcaggaag ataccttgca tggttcctgg cacctaggag aaccccactc 61801 aattgtgatt ggattgacta gagtttccat tcaaaataag agagggcagg acgtggatgg 61861 gagcatgaag gccactgaaa acactaaaat ggtaaaacaa agtttctgcc tataaattga 61921 ctaacttttc tgaaggaaaa gtctctcttt tctagagagg attttctcaa ctagcagggg 61981 atagatttgt tgaagttaca acttctttct gtgtgtgaac tgccccaatt aatgtagctc 62041 agaatcacag aacaaatgaa aaatatatag tgaaattgat ctcctttatt tgaaacttaa 62101 aatgggtttg tggaggtaaa gcaggcaaaa agcttgatca gagctttata ttttatactg 62161 aagggtgcac atgttgatat cttgactttt ttgctaaaga tgcttctgat ttccatatga 62221 agctctctat aacccagctc ctgccctgga acctcaccct attccttcac tgaaaacgtc 62281 atctaatcat catctgtcct tttcgattat ccagctatct acctcaggcc ttagtgcctt 62341 ccaagccctc tcctccaaat acttccaaag attgttttat gttttcaaat aaagcacttt 62401 taacattcat gaattcaagt taggaattat atatatgctt tcctttcagt aagcaacaat 62461 ttcccaacaa aattcaatat ataattactt ctgctttata aaaatagaag gaataataaa 62521 ttgagattat atttttcatt aaataccaag tcaaaattca catgcatgca atcctatttt 62581 tatttgctca gatcattacc aaccctagtt caaaaacatc cctgcttatt tttggacctg 62641 tgtcttatta ttttcaaatc attgaaacaa tgggaaagct gatttactga ttaaaaaata 62701 aactaggacc caaccattta aagaagcaga gattgaacca cataagcaag tagaactaaa 62761 aaacagtaaa caattctcgt cacaaagtta taatgctgca ttacacgcca gtatttttcc 62821 cttacacata tgtttgtgag gctgaaatat tctctctcaa taaatttaat gatgtttaag 62881 tcaagaaagt atagtattgc cagcatcatc tcctgacact gctgggaata ttgctgcctc 62941 tgattaacaa gagagggtta aaccccaggc acttgccctg gtgggaagtg agacctctag 63001 ctttgcatga atgaatgtaa cttaatttgt tgcttctttt agcactccca gctatcagtt 63061 gtgttatcta acagtgaaat atcacccctt ctttagagag agattgagga caagtaagtc 63121 aatgcttttt aatgtgaacc tgcggggggc ggggggagtg gtgacaatga catctgacca 63181 gtccatgtga atgttggcag tgagctcaga gctctgacaa acagcaacta acgtaaaaaa 63241 taagcacact ggtgtgaagg tatgcttgta aaaactccct gtcctctcta ccttttcatg 63301 agtggtactt tagcacaagg tggcagcttc ttctgcagag gatacaaatg atctggacta 63361 ggccccaagg gtgcagggac tgagggggag gggagaatag gcatcacctc cagtctagca 63421 agagggactg ccacggaatt acagcttgta aaataagcct ccatcagtct cccaggagac 63481 agccggcctc aacttgggtg ttccctgctt gttagcctca gtcataattg gtctctcctc 63541 ccatcccacg ttgttggttt tctgtgtgtg gcttttggtg tgagcgtgtt aggtcatcaa 63601 caaatacaat agcgggaggg ggcaactggg gacacacatg attgttttag agaagaaagg 63661 agaaaaggga taagagagtg ggcaggaagg ctgtgaacag aatggggaag atggcccagg 63721 atttaggctt ccgaggcacc ccctggagtg ctccagggta gggaactccc tgggagtttt 63781 gttaaaggga gaagaggcct gctgggagct ggggaccctg ctgagctccg ctgaggacat 63841 tcatccattc aacgctattt ttgtttgctt gtctctgggg gtgcctgtaa catcctgccc 63901 acatccggtg cctctcatca aaaaagaatt tttcaaaaaa tcccaatcag ggtgatttgc 63961 tctagaaagg ccacgtgtgt ggagctgaag acctaaagaa cagcccagga ccctggcggg
64021 ggtagggtgt gggtcacagg ccttcatcct ttttccatcc attgttaaaa tagattctca
64081 ctatcttgtc tctccatccc ccatctcctt ttacctcttc tcagaggaaa gctggggata
64141 gcggctacag taaatttctg atctaaagtt aactataaag cctagagtgg agatgtcttc
64201 gcatttaagg aaggttgaag atacagtgca ttgtttttgc tcagaagggg aaagataaag
64261 ataagatccc tagacccttg agtctttatt tactgtaaaa agaacatttg actgggaagg
64321 aaagagcaag aagtgtaatt aagtcaagtc gcaaattttg gggattgatt atttttttct
64381 gtttgtatgt ttcaaagttt tacattttac tcgacaaccc ctggctggcc tggttgccat
64441 ggtgctgcct ccagggctgg tgtccatgct gtcccccacc cagtaggatt tcaggtctga
64501 aatcttgttc ttttgctaca gcagggacag caaactggag ctaatggcac tagggtgtgc
64561 gtgggggtgg aggtggtcta ttctgtatgg caggacttga tgcctacgac tttgcggaca
64621 taagccagaa cgggctgctt gcaactccag gttcatggtg tttgggagga aaatgtttcc
64681 tttcctgcgc tctcttgcgg ggtgaaggtg catagtcaga aaaggccttg ctgctctttt
64741 gaaacataac tggtgtctgc acattccaga tctcatctcg gccacccctc cctccaagcc
64801 agggccacct ccagaccccc ggatgtgtag aagcatgcac tgccccgaag aaaacgcgtt
64861 gcccccaggt aacttcaggt acctcctagg gctaacgcct ccttctatgc catattgagc
64921 cttatctctt gcttcttccc gcccccctcc cacattaaaa ggcccccaaa gcattgagga
64981 gcggcttctc tttatgaagc tggggagaag gcgggcgata aagattgggc gtttaaattg
65041 gccatttgga agattacatc tcttcagcga gggtctttct ttcaaaactc gcactgaatt
65101 aagggtcatg gctactccgt acccggctct caaaggcacg gcacctttga attaggttgc
65161 ggagtggcct gggcgagggg acccggggcc atcaaaggcc cgagggaagg ccgtggggcg
65221 acacccgtct aatgacaagt aattgaagga ttcgacgccg cgcattacca gcggaacggg
65281 aggcattaat tatttaaaaa cgattagcct tgccgtgtgt cctgcaggcg tcccatcatg
65341 ttcagcgaag ataaccagcc acccgaggcg agccggggct cggagggcgg cgggcgcgct
65401 ccccagcagg cttcgggtgc ggtggacacg ggcgcacagc ccaaggcacc agggaaggag
65461 aggaggggcc accaagggtc cccacacagg aaggtgacta cataacactg accctgctga
65521 acaacgaaac agatttctgc ccacttgggt tcgaagtctg gtgactgcgg cgcagatggg
65581 aaaagctttc cggtcggggg tggtgggcaa tttccatgtg ccagttccct gtcgcatcag
65641 accaaagacc caaagtaacc cagccaggca cgggcctctc acagtgcctg ctacccaggc
65701 gtttaaagaa aagaccccag agagaatgtg gatctggYgt gggggcaggg attggggggg
65761 ggcgctggta gacacctggg gaaatacaac tttatgaggt ttcaaggggc agcagcagaa
65821 ctactatttt tccgaggtcc acgcctttcc gcaggcggcg gaaagacgcg gctggttctg
65881 ttgggtccta caaactcgcg tcctccggaa ttcccgaggg caagcccggc acgcgcgagc
65941 gtgctcctgc ccgtggaagg ggggaaatcg gcgactgggt tgcagggaga atgcgatgat
66001 aaatggccct gcgtctgctt tctgactttc tctcctggac ccagctcgta accccagagg
66061 ctccccaacc tgcctgccgc caagcacggc tgtgcgcaaa aggccaccag ccgcttcctg
66121 cagccagagc taaggttcac tcgcctctta gaaaggcgcg gcccctcccc tgctgctgct
66181 tgatgacgat ccccatcgtc tcctcggcct gcctgtgtcc gagaaagagt tgagactgga
66241 ctcgaacccc tgcgcgccaa ggggagggcg tcaccatgct ccggcctcag cctcttcaaa
66301 ctcccatgca ccacgcacat cattttctgc tgacccccac actagattct ggggccaagg
66361 gggttcccca caccccaccc tccactgttc tctggaaaca tgctgctctc agatcgaatc
66421 cccagacccg agccgctcga ggggcctgtg tggcttcctc ggggccctac caaactcctc
66481 taactgagct cagagcctaa gcggtctgag tttgcaactc tacgacccct catccttcca
66541 agtcatgcac tttccgaacc ccctgcaaaa aaacccaacg ccttgagtct cagttggctg
66601 agcttaccac cagccaagcc cgcccgggcg tccgagccct ggaagggaga aggactggct
66661 gggggagggg cggtgagcgg acaggagtgg ggtgcggacg ctgaggaggg gagccgggcg
66721 cggtccccgg gtgggcgcgc tccctacctg cggcggccgg cgggccggct ggcgggcggg
66781 ctgcggagcg tccagctcgc ggagggagga ggccgactgc tccgagccga ggcatccccg
66841 catttatttg agctcaaacc aagcgactgt tgactttagc acacaaagca aagatttcac
66901 tgcccgctag tttaaaaatg aatattttac caagatatcg atcagcgtta taaaattcag
66961 ttaagtacaa tgggatcctg gcaaaaaaaa aaaaaaaaga ataataaagg aggggggagg
67021 caatataata tctgtgcaaa tttgctgaag tgtgacgtgg catggaaagt ttaccctcct
67081 ggaattcgga tctagaatgt ttttttcggt tgtttacaga ctttgtttat tcacagatca
67141 tggttgctta aaccggcgcg cggaaagcgt ggggaaaaaa ggaaaaaaag gaggggggag
67201 agtctctgca ccccacaatg agtagagttg gggaaagcgc gcagtggcga tttgtgagct
67261 cgttatctcg gaacccgggc taggggaggg atttaagcgg gaggggggcg tgaaagacac
67321 aagaagcaca gaaaagcctc cgaaagcgcc tgctccccga catccttttt atgtattcgg
67381 acagaactat atcttatcgg ccgggatcag tgaagggggg cgcgctaagt agtttaaaac
67441 gaccctcgca caatgggcgt gccatacgaa acatagcagg ttagggcagc gctttcagcc
67501 tccattgtcc cccactcggc ccccggccct gcctctcggc tccttcgcca cgggagcctt
67561 ttatgtgata atgaaacaca tttcaggccg ggctcacgac gtgtgtgtcc ttgctaggag
67621 gagagacggc ctgattgctg gctgtggcct ccggatcccc ccctccccaa tttctcttcc
67681 ttgtgttctt cgggacaatg tggcatgtta agaaagggat ttggtggcgg gaggggggca
67741 gtggcgggtg ggaggcggag gcgatgccaa acccccctca agcgcgtggg aaggccaggc
67801 ttgttgggga aggcggcggg gtgggggatg gggtggcaga gaccccagca aggtagcact
67861 caaagacagt ctccaggtct ggggcgtagc tggaacactc caggttagga tggtcaagaa 67921 gcccaaagat ctaatcttga aaaaaggagc ctgagcttag aaacctgagg caaaaagtcc
67981 tagcctccag gctttcggag tgtttttacc caccgcaagc cactgcgcKc gggagtggct
68041 cccaagtctg cctgacccgc gacgacactg atcctgccct cgcccagcca gcagatgtcc
68101 tgggcaaaac caacaccaga ctcccctttg cgctstcggc cttggttttc actgccgttg
68161 gccgtccttt taggccagcg aggtcgtctg gggggagagg cagcatggtg tataagaatt
68221 cactctggga tttttcgtta tacttttaga gtccttgaag gctcacaaaa tacctgcata
68281 acccctggRc cctgcaaacc tggattgcca acctcaccta acttacaaga agatttgcaa
68341 gagccttgag ttttggaggg agatggggag caaatttgcc gacagtggca tcttcactac
68401 tgtcatggtc cattggccag acacccctta cctcctcctc actcccaaat ataccctatt
68461 tttaaaaaac agacttggat tgttaccctg acttaccctt tcaaaccaat tccaggagaa
68521 Katcttctaa atgtttccct acaccaagtg tctcgccatt tctgggttag Yaaggtcttt
68581 ctgggttaga aaggtctgcc atggttgtgc acaatacctg ctccagggag aagggtggga
68641 aagggtggca ccctgtgtac agaggaggct ggaagatcca ggcagagtcc caattccacc
68701 actgttcaca gtggtgaaca ggcaaagtgc tgcaccacaa atttgcaagt ttgatccagg
68761 ctcattgacc tgaagggagg cccaagatca gaagtacagc aaaaacatgt gaaattgagg
68821 cttcccccaa gagaagcaag ctgtggggcc caagagacca caacaacagg gcacattcaa
68881 acagccaagg tggaaaattg tgatatgtgg agagtgatct tctactggct ttaaaaataa
68941 acctgctgga atggtcttcc tgggatctgt gtatttcatt gtgtgtaaag ttaaccMcca
69001 aattcaaaaa gtaaccataa accatacaag ttccatttaa taatgtgctt gctttagtgt
69061 ttagggggat gtgtactgKt atctgcaaat ttgaagtgga tttagagaaa taagatggat
69121 tgaccagtgg aggtgaagat agatggacaa gtatgtgata aagcaagtat aatgaaatgt
69181 taattgcaga atctcgggag caggtccctg tgtgcctact gtaaaattca ttcaattttg
69241 gtatgtctga aaattttatt aataaaatgt tgggaaaaca gcaacactcc ctgggccaaa
69301 ataaaataca acctgcaacc agacagggta tccctccctc cttcttgaaa taatttccaa
69361 gcctgtatat tttgtaagcc aagtaaagaa aagaaaaatc tggaagaaaa gattcaaggg
69421 ataaaaccgg gtataacact ttaatataga tttgtggaac tctggccctt gcagccagaa
69481 tacacattta taagccataa ataaagcacg cagaaaccat aaattaatcg gacccgagac
69541 ctggatttca ccgtgtcaag attgggaatg cttttttttt ctttttcttg gtcatttaca
69601 acagaccctt acattatttt ttttcctgtt tttaaacaat agtacaaccc tctggttctg
69661 ttaaaactac atggttttac accgagtcac tcacaaaatt tttttttttt tttaagtaag
69721 acttccctgc aacaacagca atggaggaga acaacaacaa caaaaaaatc agaatctgca
69781 ggtgcttgaa gaagcaggag tctacacagt agtggaaacc ggaggctttt ttttaacttt
69841 atattctttc ccgttttcct ccttatatag aacgtggggt atctgtgtgg ccctctgttt
69901 gggacggaac ggctgcagcg ggtgaNgaag actgctgtct tgggggtgtt ggggtggggg
69961 tgttatggat ttcttctccc ttgcgtctct gcaacaccgt ctccccaaag tctcgacccc
70021 cacttgctct ctcacttRtc ctcgatccgg ggtgccagag ttagccgggc ctgaagccgt
70081 cgtcttctta agaggagttc ataatgggcc gggagtacac cccctggtag taggaggtat
70141 ctgcggccag gggcgaggcg tccaggcccg ttttgttcgt gaccgggccc atggccaagc
70201 tgccaggcat gggggaaccg tagccggggt agtgcatcac ctgttcgtag gccttgaggt
70261 ccattttgtg gggYtggtgg tggtggtggc tgtggtggtg ctgctgctcc gaggacatga
70321 ggttgttgat ggagaacggg tggttgaagg cgtagtggtg ttccggcttc aggtgggcct
70381 caggcggcag gcccgggtgg tggggcgggc ccagcaggtg ggccgcggcc tgctgctgct
70441 gcccgggaga gggcgccggc tctggggggc tcagcgccgc agccggcgtc cccttcagct
70501 ctcccaggcc ccctcgcttg tgctcctggc acggggaggc gctcgagtga ggcgactcgg
70561 tgcccgccgg agtctcggag gccggcccgg cggcctcccc gagttgagcc tgtgaggcct
70621 gggctccggc ggccgccttc ttgccgctgc cggcggcgcc tgcggcctcc ttcagcgcca
70681 gctgcttctc gcacttgaag cgcttctggc ggcgcaggta gcagccgttc tcgaacatgt
70741 tgcccgagtc agggtgcagg gtccagaagg agcccttgcc gggcttgtcg ggcgagcggg
70801 gcaccttcag gaaacagtcg ttgaaggaga gcgagtggcg gatggagttc tgccagcgct
70861 gctggttctg ccggtagaag gggaagaggt ccatgatcca ctggtagatc tcgctcagcg
70921 tcagcatctt gttggggctc tgctggatgg ccatggtgat gagcgagatg tacgagtagg
70981 gcggctttgc gtgcgtgtag ctgcgcctgt aggtcttggg gtcgcgggcg cggctcaggc
71041 ccgcctgccc gtacatgggg ctcatggagt tcatgttggc gtagggggcc aggccgccca
71101 tggccccggc cgcctgcccc ccgagcgggc tcaggctggg actcaagtgc ggccccatgc
71161 ccgccacgcc ggccgccccg gccgagccgc ccatgcccgc catggcgccc gcgccggggg
71221 acatccccgc cagggacggg ctcatgccag cgcccacgta cgacgacatg ttcatggagc
71281 ccgcgctcat gttgcccgag ccgctgccca tggcggccgc cgacatgctc atgtacgtgt
71341 tcatgccgtt catccccagg ccggcgttca tgttgctcac ggaggagtag ccctgcggac
71401 agagccccgg gagggaggcg acagcgttag caccgcggct ggagggtgcc cagacctccc
71461 acccaccgcc caggcctccg cgtccgggga ggcctccggg gagccctttc gtccccgatg
71521 gcccagtctc cggactccga gtctgtttca ttcagggaga gtcatgatcg atcgcccttg
71581 ggaaggtggc gagcggattg ctgcttgggc atgtggcccg ttcctagccc ttctccacct
71641 cgcccagatt ctagaacagg ggtcccatgg agtactccca agccagcacc ccaaatacat
71701 tcccctaccc cagagtctcc ccaaactctc ccaccagcat actgagctgg cgccgaggcc
71761 cgaagtcccc ggacgccacc tccggtcgca gcaagcaacc tgcgggacaa cgcgcggggc
71821 gcctcgggct gcctccagcg aagactgtcc cgggccccct ctgtccggtc tccctccagg 71881 gaccccctcc cttgtcccag ggaaactgca aggcagtgcc tgtgcggagt gcRggggcgg
71941 ctggaggcac tgaacggggc aatagggaaa gaaccgagac ctgaaccaca cgcggctgag
72001 ctttgcaggc aaaaactttc tttctctttg tccactagtc tttgactctg cctccacttc
72061 agcccccaac tcctacccat cttctcctcc tcagctcccc accccctcgc cggccgacct
72121 cccacccctc cccagccgcg cgctgccaaa cataactctg ttaggatagt gcgtggctcg
■72181 gccacgaaga ggatttggag gcgccgcaag tcaatatttg atcacaaagt taatattatc
72241 tcaaggctaa cagtgtgtcg tataaaaaag agacccattt gattccaagg agggcggaaa
72301 aggcggctgc ccagaaaggc tggggttgtg gggcggggtg gggggstgcc agcgagggaa
72361 gcggtcctga ggttggggaa ggagcgagcg ccgccgctcc acttccccct ggaaaagacg
72421 agcgcttacc tcgggctctg catagtagct gctccagtcg gacggctcgt gcccttccat
72481 cttcaccgct cccagcatac tggaagccga gtgcatggca gtttaaaatt taacagccac
72541 aacaaacgac cagcaatcac cccccacccc caccctcttt taaaaaaaag tcagccaaag
72601 caccgtcccc tcctccctcc ctctctcgcg ctccctctcc ctgggctcca ctccctctct
72661 ctccctgggc aggccggagg cggtagttgg aagtgggcgg gaggtggggg ggggcgagga
72721 gcggaggagg cccaggccag cgccccgcgg tagggagcac ccgccgccgc cgcgctcacg
72781 ggctgccggg tggcggctga ggttggcagt gccgagctgc cccgaggcgg cgggaagcgc
72841 gcggcgcggg ggctagtggg ggggtgggga ggaggaggag gaaggaggag gaggaggcgg
72901 tggtggtggt ggaggaggag gaggaggagg aggaggagga ggaggaggag gaggtgtgga
72961 ccgcggagcg gacaagtgcc gcagtgacgt gggaggctgg tgatatagcg cggcgcgctg
73021 gcgcgggcct ccaatcccca gacccggggc agcccatttg aataatcagc tcacacctag
73081 gtgagaggta gccgcagccg gcgctccgca cctgcccctc agcgcctgcc gtccgcccca
73141 ccgccgcggc gccccgcact cctgggcggg ccaggggagc gggctgggcg ggcgatcggg
73201 cacgcgggat ccctggtcga gccccctttc ctcccgggtc cacagcgagt cccctgagga
73261 aggagggacc tgggaggaaa ccaccctctg gggcggctcc ggcctccagc ccccgccccg
73321 tctcatcgcg ccgggcgccc ggtgcgcctg tcggagggcg cgtccagcct cagtgcccgg
73381 cagatcccta tgaaagccgg atttatttat gccgggtttc ttcgctctca gtgctcattc
73441 tctgtcaaaa cagcaagtcc atgacagcca atttgcaaag cgctgtccta tttagaaaaa
73501 ctacgtacac acctttaact cgcccgctgc tgctcctgcg aggcccctcc ctgttacagt
73561 tcagacccgg aacggcctcg ggagaagcgc ggggcgcacg gtctggccgc ctcggctctc
73621 cgactcctca gacaccggcc gccagggacc cgcagtgggg cggccggcgc ctggcgcaag
73681 cagcccctct agcagcggcc gacggttggg aggctgagat ttgYctctga tattcaggcc
73741 ggcgtgtttc aaggttactt ttcagtcaca accgaggtgc ccacagcatt tcgtaactaa
73801 aacaaacagg gcaggaggtg gggagggagg ggtgaggaga gaagaatcca ggaatcaatg
73861 atggggcata tggagttgac ttcaagagcg tctctgtcct ctctgtcttc cagagggact
73921 gcttaataga aattagaaac tccagcaatc gtatactttt atttccccag cagctcttgg
73981 gttcaaaaat tgtcttctgt tctgggttag cctgtgagcc cagatttgga gtggggggat
74041 attgcaggtg tttggatgtg gaggctcttt cttggttctg aaaacctggc tggaccttgg
74101 ggatccagcc ctgtacccac cccctcagac acagagaatg cataggaact agtgggcatc
74161 cagcccacct gtccaaaatt gggggcgatg gtggcagaag acaggcgaga tggcagacac
74221 ggagcttcgc attgaagctt gagtaagggt gaggctggaa ggcaagcgag gaggtagcag
74281 taggcacagg ggaaccactt ccctaacccc aggactctca ggtctgcacg gtttgggaca
74341 cagaaggaag ccgccggcag catcttttgc tgctgaaatg cagatggttt ggacacttca
74401 taaagagggc attaataagY ccgccgagtg agcgccaggg cgggattctc ccacaaattc
74461 aaggtctgta tctccgcgga agccgtgcct atcggtcccc cgagtgctga aggctcccac
74521 gaggggcagg ccagcccggt gcgccaggga aggagcttga tggtgtgtac ggctacgtag
74581 tgaacgtgga ggtgtgcgca gcggagtcag aggggctaga tccccagcat ttcccacggg
74641 gtcccaccta gcgcgtggat gggagcaact tcatcactcc accgagggca gggaaagagg
74701 agtaagcatt ggagctcaaa gaatgagcaa tttttcaaag ctttctccgc aggcctgggc
74761 agacacacac gcacacacac acacaccgca cccccaaaac tcaaaagaat gttatggttg
74821 acctagtctg tgcgtgtgct gtatgagagt atttgtgagc ttatgtgggt gcccacttcc
74881 ctttctattt tgctcctggg tggaaatttg gtcccagaaa ttattccagg aacagaagga
74941 gcgagctgga gggccgcagg agaagggggc gctacacttc caaggagcgg actttggaag
75001 cccccacctc caccacagca ctattggtgg ccggggtcag ggacaccaga gagcgctcgg
75061 cgggggcttc cacagtgaca gggcgccccc tgtcggccgc caggcccgcg ggaccgaccc
75121 gagtcctcat ttacccccgc gatcccaacc ccgcagctcc ggcaccggag caccttctcc
75181 cccgctctcc cagtctctcc gaaaacgMct tcggggggac tcggcctttt tggagcccca
75241 acccctaggt tctgcgcgaa cccaaaacgg cctcctgcac cctggcgagg ttctgggagc
75301 tccggtaggg tagggctgga tgacgcctcc tagagtctcg gagcagccgc tcccgcaccc
75361 tcccggcacg cctgcccatg ggaatgcgag cggggtctac ggttagcttt aggtagctgg
75421 gagcgggaca ccaacgggct gcgaaggaag cagggacgtt cagacagcct ggggttcgcg
75481 ttcccttcca gctcagcggt ctttggagct cctccttccc ggagttctga tttctaaagg
75541 gYtatccaga gttctggctc aggcttgaat gctgaagatt gctcccggag tttctctcca
75601 ggaaaaagtt cgcggggacc tcgggctgca gatgtttctc ccaatccctc tttagtctac
75661 cctcatgtag ggccgagacc tacagagccc ttccctttcc attttgttag gtcaccgccg
75721 gcttctataa taaagctgct ttataactga ggactgtgct tggggttagc atgctatttt
75781 tattttggat gttgcctcaa tggcaaagtc ataagactcg agttcaaatc caggcctcct 75841 ctgctctccc tgggaagaaa acacccaggc catccaggtc tcctaggcct tcatcttgct
75901 ggaacaatct gcgttcccct agcaaaagtc ctccctagag ctctctttgt cacatgaaac
75961 caaccagtgc agaaggggca tcattgaaca gaactcaatt ttcctaagtg agaacccatg
76021 aaatacctaa ggagaaaaac aataaaagtc ccattaatac tccaattcct aaaacctttc
76081 agagaataaa tacccaggca aggccttgct caaagttccc cagcgtgttt agggcaacat
76141 aaggcagata tgtaggcaag atgaaggcag aggggatgga aaagtgacag cacaKtcttg
76201 ggaaagtatg catttttact ttcttataaa ctttttaatt ggtaaaaaag cagatgtcca
76261 aacacccctt attcattctc tggatgaatc tcaagtctca atgaagaaaa ggcatatcgt
76321 gtgtcaaaga ttataatcaa catttcactt tctttgggtg ggttattatt tctgaaatgt
76381 actgaaaaat cagccaggcg caatggctca tgcctgtaat cccagcactt tgggaggccg
76441 gggcgggcgg atcaccttag gtcaggagtt caagaccagc ctgacaaaca tggtgaaaac
76501 ccatctttat taaaaatacg aaattaacag gatgtggtgg cccatgccta taatcccagc
76561 tacttcgggg gctgaggcag gagaatcgct tgaacccggg aggcagaggt tgcagtaagc
76621 tgagatcgca ccattgcact ccagcctggg caacaagagc aaaactcaat ctcaaaaaaa
76681 aaaaaaaaaa aaaaattaga ccatcaaaat ttgtctactt ggcactgagc acaccttcaa
76741 acacttatgt tggctttcaa agttcttgga tggtcaaaag accagttgtt tacggagagg
76801 gcaattgaaa aggtcattag ctaagaacac cttatcagag ggtatttatg taaaagaata
76861 aaaaaattca gcatgttgca gccttcagca aaggctcagg aaaatgctga taaaggggga
76921 ggcaaggtgc aacattttaa gtttaatact gtgtgcacct gcatgcaatt tatctctcca
76981 ccgcaagatc cacaggaaaa gatgaaacat acatttttaa aatgagaaaa taaaatgaat
77041 taaataaata cgatgttagt aaccttgatt atgtttgggt ggtaSaatta tatcttcaat
77101 ctatgttttt aSaagtatgg gtggtatcat tttatatcac tactttatta ttacattctc
77161 ttttactatt ttatggtgaa atatgtaata aggatattgg atctgatgtt aaggaaactt
77221 tggcaaaata acttgtgcaa atattcattc agttctcatc cttgaaagga gatcagagaa
77281 gccaccatac aaactgacga caggtttgag ttctgaaaaa caaggcatta ttattttgtc
77341 ttactggtat caggggatga actgtgattt tccctgagtt gagagaggaa gagaagaaat
77401 attcatgtca accacaggta ttgacttacc agggtggttt ctgcatattg tttaggggaa
77461 ggagacagac tctgtcctct gggtggggtg cctttaagat ggatctggca cacagatctc
77521 ttgctttatg catgtagaat ttgatcgttg tgccgaaggt tgggtttcag ttatggattg
77581 tccatctttt atgggacttc tcagtggatg gattgagcag ttgcggattc cactcccatc
77641 tgtggaggtc tgtgaagaaa gccagtgctc tgcccattct gggccagggt ttgacaaata
77701 tttctggtat atatttgaca acctgttcac cattcttgtg tttttagcac atttctggta
77761 tgtgggacta ttatagcagg taattaaaat aattggaaga gataaaaata aaaaatgaca
77821 agttttagta gcaataacaa catgccaacc tgagtgagaa taaacttctc tttgagggat
77881 gcttaaatta gatgcatctg catttgtggc cctgggagct atttcataca tttggatgtt
77941 attcttggga gaaatctcag ttattcctgc caaacaggca agcagtaagc ttcatgaagg
78001 acaagagtca tggggcgtca gtcatagact acctggagaa tccaaaccca ggcattcttt
78061 cttaggatac cattaggcct atgagctgct gattacttag ttggataaac aaatgcactt
78121 tcaacaagta tgaaacaatg cagcatacga gagacatgga gatcagaaag aagccactaa
78181 gtcctgggtt tcctggctga tgcttaggaa gcagttgttt gcttctcgtt tccatctcaa
78241 atggtacaca gtctctgaat caacaaaaag agctatatat aacttgctct aatattaagc
78301 aatgacaagg atggggagtt tatatgtttt ggattcattc agcaaatatt taatgagcac
78361 ttactatatt ccagatggtg gacaaatagg caaagccatg ggcttctaga aactaagtct
78421 gttttcctct tttacttcca ccttttctcc ctttttctgc ctcaggtccc agcctcttta
78481 cttttaagcc ttcttccaat catgaccttg gaatttccaa gtgctaaaat cctattccaa
78541 agggaaaatt atacatttca ccattctaaa atagtctagc tgcgggcaga gatggactaa
78601 aacccaaggc gaagagcctg gtttgaccca gaatcaatag aaaaaacaag ggctcagctg
78661 tcatatagac aagcagccct gcagtagccc aattctctcc tgtcRccatt ggcaattcct
78721 gtacaatatc agtttcctat caaagggata actaggggtt tctagcttcc aggagaaaga
78781 aggatgaagg tggccagagg tacWgctKca cgcggtggat gctgcttggg agcctcgtcc
78841 tgtgtcaaac atagctccta atgctttgta atgcacgcat ttgcattatt tagctctttg
78901 ttaattttac tcccaagagt aattccagag gaaggattgt ggagttctct gcaaaaagga
78961 gagcttcagg gaggggtacc atatgacagc aacagtgacc cactaggact gggcaaatga
79021 actaggactg ggatggcccc tcacaccctt atgtgcatag gaatcccttg gtgatcttgt
79081 taaaatgcag attctgattc cgcagggagg ggcttgagtt ttgcattcct aacaagcttc
79141 caagggatgc tgatgctgtc agtctggtcc actgaccaca ggtggaatag cacagcttta
79201 aagatctgtt tctctcatgg atcctgtagt ctgtccctag ctgggtggtg atatatgact
79261 tgataaaatg agaagtacag cttccttttc agcttctgca ggttccctag Kgcatctacg
79321 tccttcccct ctcagctgct ctgagccaca ccaactcttc tcacagccac agttcatggt
79381 ccccagtcca cagaagtcag aacttgtctg tgcagcccaa gcaatgtagt ggtcctcccg
79441 caaagcagag ctgcccaccc cccatgcccc ctgtggtgca ttgttagctc accccacact
79501 ccccctccta tgctgtgttt tccggagaca gggacctccc tggttgacag ggtaggaaag
79561 gggacctgtg gcaattatca gctcacaatg tggaaacatc attttaaaag tatatatata
79621 tatataaaag ccagactgag tagtgtcagc tgcaacctca ctgcatcagc aaacactcac
79681 caagactctt ttaaattttc tccttaagga agaggcccca gagaacacag actgatcgtg
79741 tccagttgcc cacctctggg gctgggctgg ccctgaaggc tatgcagggc ttcccactca 79801 aaagaaaaaa gggataggac cgacctctcc caagcatccc ctgaacctct tccttgatcc 79861 caatatgcat ttaagaaaca aaatagaaag atcaaaaaga agggaaagtg aggaagaaat 79921 tcctgcttat tgagtttcaa atgagatttt catggtcctc ctggaaacga cctgagtctc 79981 ttgtgatggc aactggaaat gggaaatttc ctccacaaaa tttggactga cattgagttg 80041 gcattgccta cccatgcatg agaaaataac attattagga aaaattaaaa ctgaacccat 80101 agatttcaca attaccccct ttgttaagaa atacaactgt tctccttagt gcaccactca 80161 attttttccc aacatatata acagaaaatg ctcagaaatt aaaatttgtg tctcaaagat 80221 gagaagaatt tttcaaatta gg-ccattccc ttaatgctta tttaatttgc aatatacatt 80281 cataattagt gataagataa tttttccctt tgggggaatt taaatgaagc attcattctg 80341 gaaaggttcc tttccttcac atatgaactg ctaaaagaga cagaaaatat ttctttctgt 80401 gggcaaattt aaaaaattcc taccatattt ataaaggcac ataaataatt aatgtagaaa 80461 cactaaaaaa tttagaccaa gaattaatca atgtgtcctt ttaaggcaat gtggattggg 80521 gataataaaa ctggtgaaaa attatactaa gctgctttga gattcctgga tggaaccaga 80581 tataacacac aaacttattt ttcagttgtc cctttcacac ctggctacta aatcacccca 80641 taggttgcct ctgttagtct agaaggttgg aatatgtgat gctgcctggg aatgaattat 80701 actatgttat atgaaaaatt atgatgtaag tgacacataa tattctccgg aggagtctac 80761 atgaataagt ctataccttt caaaagttta ggagtttaga tgtgccgttt aagtgtgaaa 80821 tctggcaaat tcattctcct aattcttcaa aataaactgc taagtagatg ctaatgttga 80881 gaaacatggg tcagagttcc ttggggtggg agctctgatc tgacactcac tatccccatt 80941 ctcatagtta agtagaaagt gaaaagagac tgttttatct ctactcacct gttttctttc 81001 attcttttct ccctcttaac atattaaatt tagaaataca gaattctttc ttcatgtaca 81061 ttacccaaga gtttccttta cctaccctcc tccaaatgac tgagctctca aagactagag 81121 agaagtcagg gtagaggcta atattttctt cagacttgat gattcatgca agccttttac 81181 ataagagagc tcactttgtt tttgggaaaa ctggggtgag ggacattgat ggctcaccca 81241 acccttctga tcacaatcta ggccctctga cccaaatctt gacctgtttt cattttataa 81301 ttttcttttt ttcccaaaag gcattccttt agttagatgg agaagttatc agaaacctgg 81361 gagaaaagaa aatactcaga ggtaaagttt ccaggttatt ggaatagtgt gcatttgtac 81421 gtgtgtgtat gcatatatgt gtatgtattt aatgtgtgtg tgtgtgcgca gtttatctac 81481 aatataccca agatcttatt gctagtgaaa tatagttaaa taaacaataa cccaacttta 81541 ttacctagta tcgtctagct attataagca gtgatatctc tatgaaataa tataaaaagt 81601 atgatgattt tataatgaag agctaaatag ttgcaggata tgagtaattt gatttaagtt 81661 ttataaaaat aaaccctgat tctgtcactg catggaaaag gattgaagtt tacacatcaa 81721 aatattaata atgatgagct aaatagttgc aggatatgag taatttgatt taagttttat 81781 aaaaataaac cctgattctg tcactgcatg gaaaaggatt gaagtttaca catcaaacta 81841 ttaataatga tacacctaga gcgtgggact taggaggatt gggagaggat ttccacattt 81901 tactttatat cttccttaca ttggacaata atctcttttg cttttgtcat aaaataattc 81961 taaacaaaca ctgataagtg cggtcttggc caccagctag ctgacatgct gcacataata 82021 tttctggttc ttattaatca aaacagacgc ttttctgcat cgtcttttag agaaaatgac 82081 caggccccct cccctgcagg aaaatagttc ctatttataa gaggaaaaat taaattcgga 82141 acacccacag gccaggagaa gttaggtagg aaaaagaaac cactaagact taataaatct 82201 gacttaattt taaaaaaaag tggttgtcac ggggcagacc taaagaacca gctagtactt 82261 aaattgctgt ggctcttaat tgcttatggt cctcaggaag tgaaaggatt gaaatgcagg 82321 ctctatgtgg cttcacttaa ttaatattta tgacttcaga agaaaattaa ataaggcaaa 82381 tggtgtactt ttaaacagcc tgtggtcatg gttagcagga ctcacaacaa attcagaagc 82441 aagaaatgca agcacacttt gggcatgcgt ggtttcagtt ttcatttttt atgcctggtg 82501 atgcctctaa taccaacatc ttggaacatt atggtgattg ttttcactta tcatgctttg 82561 catgtttctt tgtctttgtg attggttgtc tgaaggaaga tttctttttc tgggggtgga 82621 gagataggga gatgcacaaa gatgttagct ctggaagcat ctataatttc tcagtggatg 82681 tttttaagat gtatatctgc catttactga cttttttgcc tggtttgatg atttgagttt 82741 ttccattttg agagcagaga gttgatgaga tagcatcagt tcctgtaatg aggaatctgg 82801 gctataagtc tgtgtgataa tcaatgttta ttgaatggat ggatgggtag atgtgtgagt 82861 ggatggatgg gtggatggga gaatagagca cagattctat gttacttgca tttaaaaacc 82921 cagtttacct gtgagcactg gttttgaaga gatagaatta gataataaca aatcctagat 82981 ggaagctttt caaacttcca tttgtgaccc attaatgtgt gtgacagcaa tttacttcat 83041 tacaagtact ggaaaataaa ataatggaat agaagttctc agtgcatctc acatagtaaa 83101 cagagtcttg gaaaacctct gtttctgtta taaatgtgtg tgtctgcatc aggatggaaa 83161 atgcattcct tactgtgggt tctcatctag aaagcctaag cgaccgtata cactgggagc 83221 tgagggtcca ttagctctcc ttgcaaggaa gcttcagttt tctcatctgg aaaaggggga 83281 taatagcaga agccacctct tcagactagg taaggatgac atgagtggag gctcatggtg 83341 atggcttagc ccagggtctg gcatattgag aacatttaat caatgttttt tttttttttt 83401 ttctctcctg tatcctctgc agcttctcag cattacagct gagaaagttc tcaacatttt 83461 acacagagag atgcattata ctatgtttta taatttgtcc tctcaactct tacttaaaac 83521 aaccttctta aaaaaataaa cacatttgct caaaatgaca ccacacattt gaaaagatta 83581 tttctcatct ctatacatga tatagtagct gtgctgagct ctgggccaaa gactgctcca 83641 gaattggaaa acatgttcta cccacttgtg actctcccta gccaagagcc agaccttgtc 83701 ttcaattcca tggctttccc ttcgtttgaa ggttaaagtc agcatttcac ttttactgct 83761 gttaataact cccaactttt ccatatttcc atttttttct aaactcttta ctacatgcta 83821 acactgcctt cattttttct ctgccctctt cctgaggtca cttggtccta gaagcctcag 83881 Sttccaaatg tttttgcaac ctccccttta ccctccccgg taacatgctg ttcaccattc 83941 cactttctga tttccttttc tgttccttta gtagtctctg cacttcccct cctctcccat 84001 ctgtaaagcc atctttcttc caagtgatgt tctccaggtg acctgaatct ggcagaagcc 84061 tcctccatcc atctttttag aaggaccctc agttgccaga gccagtctga caccYtcttc 84121 cataccagct gcttcagacc agcctggggg cctgctctgg gggtggaacc attgtcaagt 84181 ttcacccatc agctaaaaac atgccacttg gattcattgc aggtaaccaa gaaagtcata 84241 ctctttaagc tcaccaacat agttacatag ttcataaaaa atattgctcc attttccaag 84301 ggactttctt agttgacgag tgggagcaga ggtccccata atcagcagct ggtttgctca 84361 ttgctgggaa acacagaaga caggtcggtc actggacagg gggtgcagaa cccagcgctg 84421 gagtgcacag aggtaggttt tgagaaggag tatgcacttc ttaactttcc aatagcatta 84481 ctcagtattt tcttttcctt aaatagtact ttagctcact tatcagattt caacttactt 84541 ttacacacta aagtatgact tgaggtcttc ccaggttcac acgtatagaa tgaccttttc 84601 ttttcagctg cagtgcagtg tgtatattcc atagttcttt agtcagtcta ttactattgg 84661 tgtctcaggt gagttcccca gaagcagagc ctgaggagag gactcctgtg catgagaatt 84721 accaagtcac tgtgcttccc agagagttct gtaagggagt ggggaaggca gggaagccca 84781 gtgtgggcca tttcaggcta tgcccagcag agaggagctt cagactggtc ctgaagggga 84841 ctcaggtgtg tgagtgaggc tcagcgctgc acccatcatt ggtttaaggg ccctcctggg 84901 ggcaggggtc tcacacatcc caggcacgtg aagctgtcta tgagagtata ccaggtggct 84961 ctgggcaccc aagagcagtc tatgatgaag agcttgcagg tgcagaagct tgctgaaagc 85021 cccacagcag atggccctct cctttttaat tgcagccaca gttgtgacag gggtgtgacc 85081 taatgttggc cttagctctt atttaaaggc acgtttgttt gtttcccaga gaagctcagc 85141 caatattcat ttgacaaaca ccaaatccct agtatgcccc ataagctcac atcgcagctc 85201 atggggcagc atgaatgtcc tccaggagtt cacacagctg ggagtaaacc agaaataggt 85261 atgaacacaa caaaattcat tattgcagca gcaagactgt gcaccactga gataatagca 85321 attcactctt ccttggctgg aagggagggt tgcctcttgc ctgaggggct gatatttaag 85381 ccaaacactg gaggaaaaga aagtttgcca ggccgaaagt gacacagggc tcctggagtt 85441 ggggttgggg ggcaatgcca gcacaaagac aggggagcaa ctgggcagcg ggtgttaggg 85501 caggagcttc ctgggaaggg cagcgagaac agctcagaga caagaaagaa ttgttctgag 85561 gagctttcag gctagggggt ttggactttt ctcttgggaa tggRgagtca Yagagtgttc 85621 tgagcaggag agaaaagccc aggatgggtt tgcaacagct cctctctcag taaggtggag 85681 ggaagtgaga ttgaaaccag aatcactatt cagtagacac tgaggtgtca ctgggcttgt 85741 tggtctgctg ctgggagtac agaccacaag ctgccttcag ctgtcagccc tttcaggggt 85801 caactcaact gcagagagca cttcccttga ggtcatggtc ttttctaggg tggcccaaat 85861 ccagtcctgt tggatgagag tctacaaggg tctggccatc tcggccaaac tcagataact 85921 ctgagaacta taccagctct ggggctcacc atacggttgg ccaaggctgc tttggggacc 85981 agattctccc tctgctcact cctgccttct gtccgtctcc ccaacccctg ccaattgacc 86041 ccactgactg taaacacacc acatgctaaa ctccaattga gaggctgcct ttttggagcc 86101 cagctgagac aacaactcag gaggctgtag cactacccca atagagagga tgggtgtggg 86161 aacatcccct ccagtttcca cactcagtgg agacagctca gttctggcag gagagatcta 86221 cctctctggt agatcaggcc tctgatggtt tctccagaaa actccaaacc cacacagaca 86281 ggaaagtgtc aggtaaaatg aaaacaaaac ctgggctcaa aagttctgtg tcttagtttt 86341 ggctctccca gaaatccact ctgatgcaaa gatttgagtg caagcaggtt atttggccag 86401 gaaatgctgg taggggagtg atgaggtgag agaggaaagg gaaggcagcc aggcaggggc 86461 ctgccatcag gcaggttact actgtgggca ctggtgctta actctgctgg gacctctggg 86521 agccagagta gaacatgtgc ctcagagttg tccctcgcaa gggtagggga gctgaggtat 86581 ttatgcacca actcctgtca gtcactgact gacaatggct gctcaggtgt cttaattctc 86641 caggtacttc cagtcctttg tgggtaggat agagttctgc tgctagaaat taccctgaag 86701 cacaaaggtg cacatggtgg caggtggaag gtaggcatgt gggatatact tggggcttgg 86761 atattgtcta agttttagtg tctccatttc ctaatggcaa gagcttagag gcctcatcca 86821 tgcagagctt cagctcttta gtcatcaaac aaagataatg atacatgtgt tttaattgtt 86881 gtgaggttta aatgtgaata atatctttaa gaatggctga agggagagac cagtagacat 86941 ataatattaa tcacatttcc tgaactccca aactgcattc tcaagtatgg cgaggccatg 87001 agtccccttc aactcattta agatagaatc aacttggcca ggtgtggtgg ctcacgcctg 87061 taaccccagc actttgggag gccgaggtgg gtggatcacg aggtcaatag atcgagacca 87121 tcctggctaa cactgagaaa ccccRtctct actaaaaata caaaaaatta gccggtcgcg 87181 ggggcaggcg cctgtagtcc cagctacttg ggaggctgag gcaggagaat tgcatgaacc 87241 cgggaggtgg agcttgcagt gagccgagat cacaccactg cactccagtc atgggtggtg 87301 agtcacctgc attcaccaaa agtgatattc taccaacgat ctctgggcct attcaatgag 87361 gtattggaaa gatatctcag cattcagaaa tgtttctgcc ccatttttta gggataacat 87421 ttaggaacag tgaggtcagt gtcacctgga gggtcagcag ctgaatgcag aatggagaac 87481 gcttcttcct ccctcaggat gctcactgag cccaagactt cttttttttt tgaggaggag 87541 tttttctctg tcacctaggc ttgagtgcag tggcacaatc ttggctcact gcaatctcca 87601 tctctcaggt tcaaatgatt ctcctgcctc agcctcttgt gtagctggga ttataggtgt 87661 gccgcaatgc ctggttattt atttatttat ttatttattt atttatttat ttattatttt 87721 ttgagacaga gtttccctct tgttgcccag gctggagtac aatagcatga gcttggctca
87781 ccacaacctc caactcctgg gttcaaggga ttcttctgcc tcagcctcct gagtagctgg
87841 gattacagac atgtgccacc atgcccggtt aatttttttt tttgtatttt tagtagagac
87901 agggtttctc caagttggtc aggctgatct cgaactccca acctcaggtg atctgcccac
87961 cttggcctcc caaagtgatg ggattacagg catgagccat cgcacctggc ccctaatttt
88021 tgtattttta gtagagatgg agtttcactc accacattgg ccaggatagt ctcgaacttt
88081 tgacctcagg tgatctgccc gctttgtcat cccaaagtgc tgggattata ggcatgagcc
88141 atcgtgcccg gccaaggctt tttaaaattt aatttaattt tattattatt atcattacta
88201 gaaggaggca tatggaaatg cccaatttga acagaaattt tctagaacta tgcatttcca
88261 atgctcagtg gtgaaagagt cagctagagc attgagtaat ggtgaactgt gttccctatt
88321 aaatagaagg taagtgattt ccctgctttg agtttcctgg tgtggtgatt cacttgcagt
88381 gaatagggac tcctgataag atactaccat cacggaaaat cttcaaggaa aggctagata
88441 tctagctctt ccctgaacac cagaggcaag aaaagggact atacctgggg ggctcaaata
88501 gactacagag gaaagcactt ataatttgac cagaagcact tttgtgcaaa ataccaggac
88561 tgtgataatt cccaccatgt gtgagaagag tcctccagtt ttcagggcca ttgctgcaaa
88621 gtactggtgg aatttctcct gtccttgtaa gaactctcat gcctgagtct cagatttact
88681 tttaaccgaa agcctttgat ttgatcccca tgaggctaat gggaccttaa atccatcaca
88741 agactctttg gaatttatct ggagattcca ccttctctgt atttctaatc agtctcaatg
88801 gctttttcta taatagagtt ttatgttcta gtcattaatc attttaattt ttaaaataga
88861 taacatttga aatactactt cccctttcct aagcttcttt cggacagcac tgagtaagta
88921 aagccttaac tatgccctct ggttgaaaaa aaaaaaaaac ccacacataa acctgttatg
88981 tttcagactt tatgatctga gcataaaaag agctgagcct ttgattcata ttctattcca
89041 tcacgagtac agcggtactg aagggatttg catgtttaaa ggagtgttca gaacaaccca
89101 tataatgaat tctcaagggt cttcgcagag gaattgctat tcatctccac cattgatgtc
89161 tgtggcgggg tctgtgttct ctggggacgt tgagtggatg ccagtgctca gcagcagcca
89221 gtcataaggc atctgggtag aggcctccat cttgcttgga cttgggaggg tagagagggt
89281 aggttgaatg gcttgcaggc tgcaccagct ggtggacagg tggtgtgtgc aggtgttgag
89341 agataggtaa tgaggttggt tccttccctt aaacaattta ttacggagca ccccctttcc
89401 taggagtcca aaacccagag ggatccaatt tcctgctttt aagctggaaa ttgcatggat
89461 caccacattt tcttgtgaga aaaaatgtga aggcaactgg cctgggaagg aaatctctgg
89521 ccaccagatt ggtaggaaat tctacattac aatgttctaa ggtctgtctt tattaaaact
89581 gcagcatatc ccaagtacct ggaagggtta tggttgagtt tacttaaaat ttgacaagaa
89641 aaggtgaagg cacaccagtc ctgcacgttg aaagctctgt ttctcaaagg gttgtgtgag
89701 aaggagctgg gactgggctg ggaaaaaact cagctttgct ttagcttggt cttactcatc
89761 aacaccacct tttccttgca actgtgtcaa cacagtgggc aggaccttct ctgttagtca
89821 ctacctttga tgtctgggga gctctgcttc cctatcccca agggtctctt atcagccccc
89881 taccaggcat tctttttcct caggattctg ttagtaattg ctcctccctg agacttctca
89941 tccagcctaa caggtggtct aaaccaatgg tattcactgg ttgaagagag aaattgatgg
90001 atagtagtgg gaaaaaacat gacctttaga ctcaagacct tgatttgagt ctgattctgt
90061 cacttatgag ctgagtagca ttgggcaggt acttaacctc cctaggcctc agtttcctca
90121 tcttcctcaa acaagatcat gctttctcct tcctccaggc atcatggaga ttagatctgc
90181 aatgcacgtg atggtgactc agagtgggcc tgctgtagat attaattagt acccagaaca
90241 ctcaatatgg tttctctgta tgttggcaat tgcagggtgt agagtagcta gatggatctt
90301 cacttgattt ggaagtggtc tgacttacat cacatgctgt gtggtgcctg agaagctccc
90361 ttagggagga tcatgaaaca gatgtoatcc atggcagctg caggccttgc ctttaaactc
90421 ccaagcactc acctgtgcag ccaggtgcct actccaggag gagaaaaggg tgccaagggt
90481 gtatttcttt tgagggtggt tattgtttcc tccagtaaaa ctggatcctt aactagttgt
90541 tatgtcatca acaKcatcat tatatgaagg aaaatcctaa cattttaagt tgatatctta
90601 gagggcaatc atattctccc acagaaaatt ccgagttaca gagaagacca attgttctaa
90661 ctcaaggcaa gcactttttt tttttttttt ttttttttag tggttatttc taaagaaaga
90721 gaaacaagct tactqtaggc attctccttc tcaacagctt gggaaagaca ttggacatag
90781 actcaaatga cccagctttg ccccagctct gtctcttggg agctgggtga ctctgagtca
90841 attggttaat tttactgagc ctcagaagct taacctttta aaatagagtg aacacatcca
90901 gttgtccaga gactgtgaag gtggcatgag atcagtggag ggtaggagct ttgccaggct
90961 ttggttgtcc cacagcttgg gggacatgga catagggaag tgcatgctga acaggtctca
91021 gaattcatct caaaaaccat aaaccttact gggcatggaa agggtttggt tcttcccagg
91081 ggtttggttc ttgcagagat acacatttgt tgatgatgaa taggagagca aatttttatt
91141 attgaaaata ttgaaccggt gcctttgtca cctcatcagt catattctct ttcaacccat
91201 ccactgtgaa agtgaaaggg aatccgtgag ggacaggttg aggagttttt cctagatccg
91261 tgcattagtg cccaggagcc acccgaaggt cttattacac attctgaatc agaaggtctg
91321 gagtgaggag gggagagctg agagtcagca ttttttacaa gcccccaggt gatgctaatc
91381 tcctattcca agggccactc tttgagagtc taaggctaag aacaaaatgc ctttgaccta
91441 ctttgaggtt tagttttctc cttcttgagc ccacagcaga gaaataatcc taaaaccagg
91501 ttttggggaa agagaaaaaa aaatttaaac tcccaaatcc tcccttacat ctccagaatg
91561 aggattgaca gactcagacc ctagaggttt tccatgagcc atgacaaggg gaggttacag
91621 gaggccatgt gaaagcactg caggcaacaa gaagcagata ggaatatctt cctgattttt 91681 gagagaaggg ccagaggaag aaggagagga gtagttgggg cccaggctag ctttccttag
91741 ggccgatctc aggaagaggg agggtgggga aacacctagg aggtattttg tgagactcaa
91801 gagacacttg aacacatgct cctgtttccc tccccagcac gaccccccaa cacggcagct
91861 ctacaacggc aaggacgggc atgggtttgg gatgcggggt tttgacactg tgtgttgtct
91921 tcttcacctc ctccatggtg aggaagagag ccgggcattg ctatgggcgt tgtgggggtg
91981 agtgcagtcc ctgagggatg aggccatcgt gcctgctgtg ggttggatcc tcagtccccc
92041 taaggtgaaa gggagccatg tgtgtggact ctccccccaa cagtcctgat ccaggcccat
92101 gctcaacaga ctcttcagca aaaggaactc acatagttgt ccccaaagaa ccaggcagaa
92161 ccggcactct gcagcggcac cagaggatat tgtctcgacc aggggagggg agatgctctt
92221 tacctgggag ccacaaatac aggagctctg catagggacc aggcctcccc taacacctga
92281 agggacatta agcatttcct ggaggcacct tgaagaggca caggaaggat aaacagctat
92341 agtggaaggt ggctttagaa tagatagcct aagaggctga ctgatgtggc ttacacggag
92401 gaggctgttt ctctccacaa gccagcttgc gcagcttccc ccagtcatta cagggaaaaa
92461 agtgggagga acttcaaagc tgagtccaat tataggaaat aaagaagtca tttttttctt
92521 ggcacacggg aacagtggtg tggaatgtca gtcctgctat agtaccattt aatcaataca
92581 caaaactaaa tcttaccaca ctaaaataaa actgtttaat tcatatgctt tatactatgc
92641 attttaccta ataaccatga cagtaaatga gatattttat catcttttaa gattgaacat
92701 tgtgttacac atatttccat atattgtcta gatttcttat tctttatttt gttatattca
92761 accttgttaa catttagtgt gaaaaatggc aaacgtgttt atgtgtgaat gtgtatatac
92821 attttgtatg taatatatat gctgttatgt gcttagttgt gtccacctaa aatttatatg
92881 ttaaagtcct aacccccagt acccaagaag gtgactgtat ttggagatag ataggatctt
92941 caaagaggta actaagttta aatgaggtca tttaatggat ggggcctatt taatattagg
93001 atgcagacac acacagagag aagaacRtgt aagatgtcca tctgcaagac aaggagagag
93061 gcttcaggag aaagcaaccc tgcKcacact ttgatcacag acttgtggcc tccagaatgg
93121 taagaaaata catttctgtt gtttaagcct cccaggctat ggtactttgt tatggcagcc
93181 ctagcaattg aatacagaga gtaatatacc tattagacat acatgtcaat gttcccttta
93241 caggaaatga tgaactcagc agtaatttaa taagagatga cttagaattg taaattcttK
93301 cccactcata tgatgaatat ttattgggga ccctctgtgg atgagctgtg gcacacatga
93361 tctgctccct cacatgtaca cctataaatt cacccaacac atggtgattt gggttgctag
93421 atgagagKca agcaaattgc ttttgagaga ggaggctcag tgaagtggca tttaagctgg
93481 gtttggatgg gagagacaga agaacatccc atttgggagg aatgcatgag aaataatatg
93541 gagcaaagat gtagacatgt tctggaaata gtgactggat agtagggtgg gaatatggac
93601 agatcataag gggagggtga gagatggcca gggaggtcag ggactggcca tattgtgctg
93661 gaccttgaaa gccaggccca aaatcctgtt tcttttttaa cagggaatga cgagtaacag
93721 aatttttcaa agctgggaag tcacatgatt aaagtggtag attaacctgc tagaaacatc
93781 tagaatgtag agcgagggtg cactagaaat aggaaaacag atggactttg cagtaatcca
93841 gatgcaaggc aactgagaag agggcaggaa cgggaggcat ccaggagccg gagaaaagag
93901 gatgctcRgg acctggcagc ttccttttca tctctaggta aattaaatac tttcccacag
93961 tttggtgccc ttcatggtgc tgctacctgc caggcttgag agagtgagaa gctggaggtc
94021 tctgcctttt ctgtggctgc ccaggacaaa cctcattggt tctgctgaat gagcatcatt
94081 agcgtattta tactagaact cagttccaga tagcagcatt tgagaatcaa gataagagag
94141 tgttattttc agtgggaaat cagtgggcca gtggaagagc tagaatagtg agtgacaatt
94201 gtcttgctgg ttactctcag cattggcaca ggggtgtggt gttgggctaa cttaatttgc
94261 actcaaatac tttgtcctcc cactggctga agttccactg cacagagatt atgctttggg
94321 tcatgtggct ctcgtgaggt gaatgtcctt tatgaagaac gagaactaag actctctagg
94381 accggctggg ataaggcctt ggacgtggtg tctatctggt tctagcccct tgagatggag
94441 ccatctattc cacattagcc tgttcaaaca gggcctccag catgctgtct tggatttact
94501 ctaaaattct aatctcttga tgaagatttt ggtggctaca aattgctgct cttcctctgg
94561 gcactggaat ggatgggtgt cctggtttgg attttctagc tcagccctaa agaggaagaa
94621 gatttctgct aaagaagaat gctttgcctg gagtttctgg tgctggcaat tggcttgggc
94681 ccactcttga actaataggt tcaactttat cccctggcca caggtataaa ctcaagagtg
94741 aactctttat gccatgtaag ccacacaggc tgcaggtatt agaaaaagag ctgcagtact
94801 cttctgaaaa gtcatctctg ctctcccttc ctgaaggtgg tggaagaaac ttgtaatcat
94861 gagagctgct gggatccatt tgcaaccctg catgatgctg accacacaga aagaagacga
94921 gaaaaacaga atccagaggc cttgacattc ttactgggcc ccagtatcaa aaactcaaaa
94981 tcttccctac ctctggactt ccagttatgt gatccctgaa aggcatccac gtgtggcctt
95041 tcccagtaag gctttagtta ggttagctaa aatgttagca aagtctaatc ttattggcag
95101 aaaaaaaatt gccttttact atgtttgtac ctttcaaaga cctcatagcc cagcagactt
95161 cataaatgct ggtgaatttc tttcaaacca ttgtcaaata aaacctccca tctccaatta
95221 gcataatgtc tccattctga aagtccagga ggcttcttta tttttttgtt ctaaaRatat
95281 ctataccagg attgcaacaa ttatgaggac tactaacaag aaaaactcct gaaacttttg
95341 agaatgggat ttgcatttgc aaaatgattt gcaaccagac ctgccaggta gcacttcacg
95401 gaaatcaaaa aatgggtttt gaatatcgag attatatttt ttggcatcca aattcgtagt
95461 aaaatcacct ttctagctgt ctgcagtaga tgtgactaaa ctctcagaat agcttccaca
95521 agtgctgaat acttttgata aaagtcgatg tagctggaac acagtcacat ttgattgact
95581 taccttaatt ctctaaatca gcttattKcc caaacagctc ctcaattcgg gtgtccccat 95641 gctgcggcca ccttctctag ttgccagagc ccgcttctcc accctaaata actgcgactg 95701 cggcagaggc atttgggttg gtttaagacg gcagtatatc cctctgtttc ttcattcagg 95761 atttctttcc ttcaaaggag actttgtgtt tcccaagttt ccttcttctg cttgtatgaa 95821 tctaaaatga atctgaacta aaagtacttg ttgagtggtt atgctcccaa ctgtaatttg 95881 gagtcaaggt atgaatattt tatactcaaa gagtcactga agatttgtag gaggctttta 95941 aaaataaaat gaatacactc attttgttgg ctcaggtaga ctatagctgg gggaaaacaa 96001 aaatacagga tgagccctca atcatgagtc tttaaaaaat gaatcaggat ttgtgttttg 96061 ggataatcag gagtccctgt ggtgagtaca cagtgtcctt tggaggaaag aaataccagc 96121 ctgaaggaga accgggaaac aaaaacaaaa gccacccaaa tgctccaatc tcgttttaaa 96181 ccaacccaaa ttccagaggt gcagtttcag aagggagctc ttcataccct ggcagaaatt 96241 aggtgtcaag ctagagagcc agtattcaga tgcctgtggc ttcctttcac tgcaaaaata 96301 gatttaaaaa ataatccagt ctttggcata ttttctccga gacggagaga cctgagtagc 96361 ccctttgctt tcRctgtatg tttgtgttgc actgagctga gggctttgtg agctcatagg 96421 ctacttcaaa agctgttgaa aacagtcaaa acagaaactg aatacatgtg aattaagcat 96481 ttcctttttt atataaatct gtgagtcttt ttttttaaaa taacatctat tggtttatat 96541 ggagggaccc cgttaagcaa ttttcactgg atgcagtttc agcttgaaag aacaaaaagt 96601 agaagaaaag tgtctaggct cttttaacct tcaagcatgg ccacacttag gaggatgatc 96661 acctgagaat cataagttct ttcctgaaga ctctgtagaa ggacatttgg attacaaatc 96721 atttttgacg ttcatcaagt actcaaatat atgttttaat tgtgcctgat gcacattgct 96781 tcttttataa tgcactaatc ttactctaag caaagtatgt agcaattttg agataaggaa 96841 gaaagtttct gagctttcat gaggtggctg tcaagatcat gaccacctgt ggggtgctgg 96901 gtacattacc cttgagaaga tgctctgaaa tgacattgtt tcttcttttc atttatcgca 96961 aagggtttca ttctttattt atttattttt tttagttgga gtatcactct gttgcccagg 97021 ctgaagtgca gtggtatgat catagctcac tgcagcctca aactcctggc ctcaattgat 97081 cctcccacct cagcctcctg agttgctggg aatacaagcK catgccacca tgccggggta 97141 attttttttt ttctggtggc ggagggacag ggtcttgcta tgttgcccag gctggtctca 97201 aactgctggt ctttagttac ccttcaccct taccatcaca aagtattgag attgcagatg 97261 tgggccacag cacctgacca agggcttcat tcttaatgtc taccttctgct cctggattct 97321 ggatcctttg aatagattcc acctgtgaga agcttgaaaa catcagatgt atgcaaatta 97381 aaaccctagg tactaatttt acctattaaa ttggcaaaaa ttctaaaatt tgttaacatg 97441 cagtattggc caaagtttca ctaaaaaata tgcccttgta gcRgtatggg aataaaatca 97501 gtaSagcttt tatggacaga ttggtagccc atgctttgtc cccacaattc tgcttctagt 97561 tcttcatcct gcaactgtag aagtgaacaa agatttacat gtcacattta ttgtcatagt 97621 gaaaacatgg aaacatcatg ctaatcagca ggagactagt tacataaagc atactcatac 97681 aaaggaattt tagcagccat gaaaaaggat gactaattac aatgcgcagg cactgagggg 97741 tctccaagac acagtgtcac gagaaaagca aagggcagat atagtttatt cctctttgta 97801 gacaaaggca cacatatgct tagatacatg cagaaaattt cctggaggat agagaataaa 97861 tgataacaac tggtaatctt tcaggtgtgg aactgacaac ctgaggggag aggaggaaga 97921 atttgctttt tattttataa acttttgtat gactttgttt aaaaaaaatc atatttgtat 97981 attatattat tataatcagg gttgtttatt tatttattca tttaaaatgg agtcttgttc 98041 tatcacccag gctagagcgt ggtggcacaa tctcagctca ctgcaaactc cccctcccgg 98101 gtttaagtgt ttctcttgcc tcagcctccc aagtagctgg gattgtgggc gggctccacc 98161 atgcctgtct aatttttgta tttttagtag acatggggtt tcaccatgtt ggccgggctg 98221 gtctggaact cctgacctca agtaggccac ccccctcagc ctcccaaagt gctgggatta 98281 caggtgtgag ccactgcacc cagcctaatt tttctttgta ataagcaaaa aaatccatct 98341 gtggccgtga ctctacattt cctgaattaa tccacactac tggattagtg tggtaaacaa 98401 gagtttacca cctggccacc tcagtctctc tgacRatctc ttaagcacga gtgagaagtc 98461 ttaattctta gcattcctgt gctcaggtct ttcctgtcat atcaaaaaca ttacgttatg 98521 ttctatgaaa ccccctgggc tccagacctc ttccctcccc gtRatgcttt cctggttctc 98581 tgaacgcttt agcagttgta tcaaccttcg tgaatgccat cgtattcgtt ttctattgct 98641 gtataacaaa ttgccataag ctcagaggct tgaaacacac acattttttt ttttttttat 98701 atcatggttt ctgtggtcag gaatcctggc acagcttagt aggccctcta cctcaggacc 98761 cacaacaKgc cgagttccag gtgttgtcYg gggctgggat ctcattgtaa ggctcagcca 98821 ggggaggatc cacttccaag ctcatgaggc tgttagcaga attcagttcc ttgccttctg 98881 ttgaattgag ggcctcagtc tctagctggg ttctagctgg gcaaggccat ctcagttccc 98941 ttccacatgg gcctaccaac atggccccca acatatccct tgctaattta ccaacaccag
[0382] Following is a genomic nucleotide sequence of a JAM3 region (SEQ ID NO: 8).
>11:133497801-133561750
1 ttgcctgttc actgtgatgg tagtttcttt tgctgtgcag aagctcttta gtttaattag
61 atcccatttg tcaattttag cttttgttgc cattgctttt ggtgttttag acatgaagtc
121 cttgcccatg cctatgtgct gaatggtatt gcctaggttt tcttctaggg tttttttcaa
181 tatatggaaa ttcacatttc gcttgggaac atgaaggRga agatatacaM aaggaaaact 241 agatgagaac aaacaagctg agtaaaagta gttgtcagaa aataaactga atgataggta
301 ggaaagcttc aaactattaa gtaaatgctg tgctttaaag ttagggttag tagcctgaac
361 atctggatcc tggagtgctt tcaagctctt tgctgagaac atttctagat ttcagtgttg
421 tctggttagc agaatctttt ctcggacagc catgtaacaa agttgtctat cttttgaaaa
481 ctccaaacta ctaaagtgtt ggaaacctga aatgctttgc tttgaaggta agatgattaa
541 aaaaaaatct attgaaatat ttgaactcag gtataattga cttgttatgt aataagattc
601 tgaacatgta tacatacaca atatgaaaga atttcaagtc ccaaacttga gtttgcaaat
661 gaagacagac atttagagta actatgcacc atggttaggt ttacttgctg tttttctatt
721 cctcctgtta agtacattgt aaactacctt aagctaatgt attggctggc taagattcct
781 acagggttca ttcttttcat tgggcatcaa tagtgtcagt ggtattttcc atttgaaatg
841 tgatggaact gagagacact gtggattggc ttagaactga accacagtta aaagtggtca
901 tctgaggacc agcctgggca acacagtgag acccccatct ctataaaaaa taaaaataag
961 ggctgggcgt ggtggctcat gcctgtaatc tcagcacttt gggaggccga ggtgggtgga
1021 tcatgaggtc aggagattga gaccatactg gctaacactg tgaaaccctg tctctactaa
1081 aaattaaaaa aattagccag gtgtggtggc acgcgcctgt agtcccagct actcaggagg
1141 ctgaggcagg agaatcgctt cccagaacct gggaggtgga ggttgcagtg agccgagatc
1201 gtcccactgc actccaacct gggtgacaga gcaagactcc atctctaaat aaatagatag
1261 atagatggat agatagatag atagataggc atggtaagcc cagRagttag aggctacagt
1321 gagctacgat tgtgtcactg cactccagct tgggtgacag agtaaaaccc cgtgtcaaNa
1381 aaaaaaaaaa ttgttgtcat ctgagttaca gctttggtag tgggtgaagt ggaggggcag
1441 agacaagtta tcacttagaa tttatagccg actaagtctg gatcaaggat gtatcatgca
1501 aataaatgtg tgcaatggat ggaaaagttg cctgaggcaa gggaagaagg gagctactcc
1561 agtgctgtgt ttgggggtgt gggttttttt ttttttttct ttaactgtag gaatttatgg
1621 atagtataaa tttgggaagc ttgaaaagtR tgcataaaaa tatttataat cttactatca
1681 gggcagtatt gcaacaattc agtatttgta atatatctac ttctagtcat ttctgtacat
1741 tttaaaaaat aattgagttc gtactctaaa tataattaag tatactactc ttttcatYcc
1801 ctttcgcaga attctttagg ggctttctgt tgctcttggt gcaaagccga aagccttgag
1861 cttcttatga ctgctctgat tttccctccc tgcagtggcc tcttgctcag tgtagttctg
1921 ctcccttggc tcactttcat tttcccacac atgcccagag ctgttgcgtg tgctgcccgt
1981 tgtgtgtgat gcgctctgca gccaccttgt tgcctggcgg agccctcctt attcatcatg
2041 ttccacctta Macgtcactt cctgagggaa gcttctcctg aaccctcctt attcatcatg
2101 ttccacctta aacgtcactt cctgagggaa gcttctcctg aaccctcctt attcatcatg
2161 ttccacctta aacgtcactt cctgagggaa gcttctcctg aaccctcctt attcatcatg
2221 ttccacctta aacgtcactt cctgagggaa gcttctcctg agccctcttt attcatcatg
2281 ttccacctta catcttattc atcattttcc atcttacatg tcacttcctg agggaagctt
2341 ctcctgaacc ctccttattc atcatgttcc accttacatc ttattcatca tgttccatct
2401 tacatgtcac ttcctgaggg aagcttctcc tgagccctcc ttattcatca tgttccacct
2461 tacatgtcac ttcctgaggg aagcgtctcc tgaaccctcc ttattcatca tgttccacct
2521 tacatcttat tcatcatgtt ccatcttaca tgtcacttcc tgagggaagc ttctcctgag
2581 ccctccttat tcatcatgtt ccaccttaca tgtcacttcc tgagggaagc ttctcctgaa
2641 ccctccttat tcatcatgtt ccaccttaca tcttattcat catgttccat cttacatgtc
2701 actccctgag agaagcttct cctcagccct ccttattcat catgttccac cttacatgtc
2761 acttcctgag ggaagcttct cctgaaccct ccttattcat catgttccac cttacatctt
2821 attcgtcatg ttccatctta catgtcactt cctgagggaa gcttctcctg agccctcttt
2881 attcatcatg ttctatctta catgtcactt cctgagggaa gcttctcctg aaccctcctt
2941 attcatcatg ttccacctta catcttattc atcatgttcc gtcttacatg tcacttcctg
3001 agggaagctt ctcctgaaac ctccttattc gtcatgttcc accttacatc ttattcatct
3061 tgttccatct tacatgtcac tccctgaggg aagcttctcc tgagccctcc ttattcatgt
3121 tccaccttac atcttattca tcatgttcca tcttacatgt cactccctga gggaagcttc
3181 tcctgaaacc tccttattcg tcatgttcca ccttacatct tattcatcat gttccatctt
3241 acatgtcact ccctgaggga agcttctcct gagccctcct tattcatcat gttccacctt
3301 acatgtcact tcctgaggga agcgtgtcct gaaccctcct tattcatcat gttccacctt
3361 acatcttatt cgtcatgttc catcttacat gtcacttcct gagggaagct tctcctgagc
3421 cctccttatt catcatgttc caccttacat gtcacttcct gagggaagct tctcctgaac
3481 cctccttatt catcatgttc caccttacac gtcacttcct gagggaagct tctcctgaac
3541 cctccttatt catcatgttc caccttacat cttattcatc atgttccatc ttacatgtca
3601 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttacatgtca
3661 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttaaatgtca
3721 cttcctgagg aaagcttctc ctgaaccctc cttattcatc atgttccacc ttacatgtca
3781 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttaaacgtca
3841 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttacatgtca
3901 cttcctgagg gaaacttctc ctgaaccctc cttattcatc atgttccacc ttacatgtca
3961 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttaaacgtca
4021 cttcctgagg gaagcttctc ctgaaccctc cttattcatc atgttccacc ttacatctta
4081 ttcatcatgt tccatcttac atgtcacttc ctgagggaag cttctcctga accctcctta
4141 ttcatcatgt tccaccttaa atgtgacttc ctgaggaaag cttctcctga accctcctta 4201 ttcatcatgt tccaccttac atgtgacttc ctgagggaaa cttctcctga accctcctta
4261 ttcatcatgt tccaccttac atgtcacttc ctgagggaag cttctcctga gccctcctta
4321 ttcatcatgt tccaccttac atgtcacttc ctgagggaag cttctcctga accctcctta
4381 ttcatcatgt tccaccttaM atgtcacttc ctgagggaag cWtctcctga tctctcagat
4441 tcacaccccc attatacact ttcgtggccc ctatactttt atagtattta ttgcaattgc
4501 aattaattat atatgtagaa ttagaatgtt gtttgtgtgt gttgtttatt tgtgtattat
4561 tagaaggcag ggaccatgac agtaggaatt gttcactgca tttcttggcc caaatatagt
4621 acgtgctaga tgctcaataa tgttcgacat ttgttgaata aatgactgaa tatattaata
4681 aatgaggaaa taggcagcag tctgcttcct tcatatgtaa cacgtatgtt aaaagatatg
4741 cttggtagtg aattctgaaa gtctaatgta gtgaatgaaa gtgtaatatt gggttctttt
4801 cttcatgtgc tgtgttaaat ttagttaaat ttcagtaatg aatcttagaa aactgtcagc
4861 attaggtctg tcaaacaaat gtgagaggca gaaaagttcg aagagagaaa gtaaaccagc
4921 attgtttccc cacttactga aaataggttt tgtaacagaa tggtgcagtc taaatgaaga
4981 aatacacctg ttaggaatac cttctaaaga tctgtttgca tgtactaatg caaatatgga
5041 aaactctttt ttttttatKg gcacatgaag tcttttgact aagatttgtt ctaataggcc
5101 cagcgcagtg gctcacgcct ataatcccag cactttggga ggctgaggca ggcagatcac
5161 gaggtcagga gatcgagacc atcctggcca acatggtgaa accccatttc tactaaaaat
5221 acaaaaaatt aactggttgt ggtggcacat gcctataatc ccaactattc aggaggctca
5281 ggcaggagaa tcacttgaac ctgggagtcg gaggttgtag tgagccgaga tcgcgccact
5341 gcactccagc ctggcgacag aatgagactc tgtcaaaaaa aaaaaaaaga tttgttctaa
5401 tattaaatac aaaatcaagc aaatcaaata gaaacaaaag agtctaggtt aaagcactWc
5461 ggaagtactc agttacgagt gtgctgttaa cttgctacat gaccacttac taaatagggg
5521 ctactttttc gccatcttag gaagaaactg agtataccag acatcttaaa cataRcaaac
5581 atcttaaatg ttgtcatcta agattcagac aggtttctaa atgtttttgt ccttgacttg
5641 caaacactca gtggacacat acagccctct aacccactgt gtctcaaatg ttgatgtaca
5701 tgatgtcagt tgccatgcat tttatttatt tatttgtttt tttgagatgg agccttactc
5761 tcacccaggc tagagtgcag tagtgcgatc ttggcccatt gcaacctcca cctcctgggt
5821 tcaggtgatt ctcatgcctc atcctcccaa gtagctggga tgacaggtgc gcgccactac
5881 acctgcctga gttttgtatt tttagtagag acggggtttc tccatgttgc ccagtctctg
5941 gtctcgaact cctggcttca agtgatcctc ctgcctcggc ctgggattac aggcgtgagc
6001 caccacaccc agcccgtgta gaaggcattt atgtaatgaa ctgacatcct tggagtgagc
6061 tttcagttta gtataaatag gtaagggttt ggattctctg atcatcttct tggtctgttc
6121 acttccatcc agaaggcctg ggtctggatt ggcaccatgg atctggttga gacaaacaat
6181 aaggcaaggg cttgtatgtt ctttctttca ctttatcgta cttcatagcc agtacatagt
6241 agtgttttat tatgttatgt tatttcctct attttaattt cttctcaaat ccaggggcca
6301 gttgtttggg cagatgttta ctagggactc taatcacctg ctcttcaccc aaggaaaggc
6361 agtctctgaa gtctcatttc tattatctaa aagctagttc tgcattttcc cttcctgcaa
6421 gcaaggaatg gttttctttt gacgtttcca gctatggttt gctttgaact gacttctttg
6481 ttgtattggg gtgtgtgctc agttaatgag taatgaacac gattggattc ttctttcctt
6541 gggtagatac tggccagaat tattctttMc ctatgctgta ccagttaata aaggactcct
6601 cagtttgtgc tttctcataa ttcaccaaat ctagcaaatc tagtatcttt aatttggttt
6661 attattacca ttaaatatta gctgtatgcc cacattttgc taacatgtgg cactttgtag
6721 aactgttagg ggactaccca gagagagtgc agtggtggtg tgttttgggg ttgtggaaga
6781 gggctggaat gtagtcactg ccctccagaa gtccctttgt catatataat acagaaaagc
6841 ctttttcttg tcccctccac accccatccc ccgcatccca tcaagctgct tagggtgagg
6901 ggaaacacca tgagagagga atggcaatag tgaatgtgtt ttgattactc tttaccatcc
6961 tcttccctca atagtttgaa ctttccttag aaacaatttc taagagggaa tttcttaagt
7021 gagtactagg aaagttggca aattttggaa acagtgttta gtgtctgtgg ttgctgttct
7081 gcatttcagg gcgccttgcc tacagattaa tcccagctgg ccactcttct acagcacttg
7141 ctcaggaaca ggttgtattg actccttcat ttgagattcg gtgaaattcg caagtacctc
7201 tggaatcatt tgatgaacct ctttggagcR ttagtgatgc aacttaatgt ctttggcagg
7261 gacatttctt ctcacatctt attgatactg ggtttgagga gcaggctgac ttctggttgt
7321 gtagtcagct ctcggttttt ttctactaat ggagaaggga ggcattatag ggaatggaaa
7381 attcaagttg caaagcatta aaaacaattt aaaattatgt ttcatttaaa ctaacaattg
7441 ctgtatttct gaccaaataa tgtttacttc aggataatgt tttcttgatt tatttttata
7501 ttacaagttg ccactttata aattctacag atcatggaaa gtagtttaaa agagtgatct
7561 tcagattttt aaatttaaag tctattctta attttctttt atatatatgt atatatatat
7621 ttatatacac acactttgca ctgtaagata ttgagtttat gacattaagg actctggccc
7681 ttcagttctt gaaaatttag tgcttataaa attgtgagga ctatattcta atcattttca
7741 gtcagtgaaa aacctatata ctttatgaag cacgcaggaa acatctcctt ttctgcttag
7801 ttcccctaaa aatgtggatc agatccatct cctgggccta gtcagtattg attgagtgta
7861 tacccagggc atcgtgctaa agtgtcaccc aagctctgcY ctccagggga agaagagcta
7921 gccattcctt ttactgtgca tgcatatgta tatagctcac ctatatccag aaaacttagt
7981 ttacaaatgg actgcttttt agggaaggag tggacacaaa agttcaactt cagagaggaa
8041 aactaagagc aatgccaaat taactgaaat cttctgtttt gaaagaggtc ttttaattta
8101 aaaaaagcta gttcttggaa aaagcacaat aatcaaaacc aaatgtacct cattagaaag 8161 ttcacgtgag ataatttggg atttgcaaaa ttagttttaa tcaattgtga aggacagcaa
8221 gactatttYt acctgtgcca ggtagaaata gtatgtatct aaagtcagaa ccattagtgt
8281 cggggcaagt atagatactc attctctgta gttgggtcag ttgtcttcct tatatgttcg
8341 ccttagctaa aatggcagag agaaatcctt gttgcatcat aacaaggaca ttgtcaaagt
8401 acactgaact catgaattag acaaatagca gcattgtatc ttctttttct gggcctgatt
8461 ttgaggtata ctgcacttcc taagggtgat ttcagagcta gcttgcaggt ggagctccac
8521 ctgctgatac ttgtgaggga tagctcagta agcactcctt cagctagcat gtttcccact
8581 aacattcaca ctgatactcc agcctagaga aagacctttg ggttacacca gtctccagaa
8641 agccttcatt taaatgcaac tagggagagg cgtggtggct caccacctgt agtcccagca
8701 ccttgggagg ccaaggtggg cagattgctt gagcccagga gttcaagacc agcctgggaa
8761 atatgatgaa atcccatctc tgcaaaaaat acaaaaatta gccatgtgtg gtggtgcatg
8821 cctgtagtcc ttgtatgctt gggaggctga agtgggagga ttacctgagc ctggggtttc
8881 gaggctgcag tgagctgtga tcatgccact gtactccagc ctcagtgaca gggtgagacc
8941 ctatttcaaa aaaKaaaaat aaaaataata ctaccaggtt gcatggggga ggaatgaggt
9001 aattgtaaaa tgaaggtttc agggaaaagc ctgggcaagc agctcttggc ctgggaaagg
9061 caggcccagg aacagatttc agagttgtcc caacaagttg ggtgaaatct gtttctattt
9121 gcttcatttt ctttttctct cctactcctc actacttaga aggggatttg agggacccat
9181 ttagatcctt ttctcactgt ctcgccctct ctctgatggt ggtattgagg ataagacagt
9241 atttgtcatc tgtaattaac tgtactgcat ttgtaaggca agttgtgctg tacttgattt
9301 ttatgtttct ttcaagattt ctgccctttg cctcctgtat ttttctattt atttgtttat
9361 ttatttaggg acagaatctc gctctgtcRc ccgcccaggc tggagtgcaa tggtgcgatc
9421 tYgggtcact gcaacctcca cctcttggtc tcaagcaatt cttctgcctc agcctcctga
9481 gtagctggga ttacaggtgt gcgccaccac gcctggctaa tttttgaatt tttagtagag
9541 acggggtttc accatgttgg tcaggctggt ctcgaactcc agacctcgtc atccgcctgc
9601 cttggcctcc caaagtgctg ggattacagg cgtgagccac cgcgcccggc ttgcttcctt
9661 tatttttcta acctcatcat tttctgcttt gctcagtgga gattttccaa taatgtcacc
9721 caaaatatac ttttaagtca atgtgtgcta ttttttaagt cacttctgac tttaatgtta
9781 gacctctcct ttgatgaaag ttaaatctca tgacatggga gagtgacatt atatctttaa
9841 ttaagatgtt aaattcttct caaattctgt ttctgattag aaaagtactt tgaggtgaat
9901 ccccctttcc tccttaaaaa aaaaatagtg gtcagttcct atctctaggt cttcagtgaa
9961 atgatcccat ttttctgatc caaacaagtt tctgtggctc atctgctctg cagagccctg
10021 tgtcttctga ggaacgatKg ctttctgcat aacatacagt gttcttYgct gctttccacc
10081 cacagagcct ttcttgctga ggaggaggtg tgatatagga aaattatgag ggcttttcaa
10141 actcgcttgc attgatggcc cagatggtga ggaaaatccc tKggggagct aaaaattgaa
10201 tgggttatag cagattttta aaatccctaa ttagactctc cttctcttta agaccacttt
10261 tgagttacca ctgtatttat atatctgagt cctgtttccc aactgctagt gagctccttg
10321 agggcatcct tgggtctgta caccactgaa atctcagcac ctagaaagca gttctctaat
10381 agtggctgat accatgtgaa tgcattctct gcRtcaggtc ctgtactcag cactttacgt
10441 atgctctctt actcttacaa ctggaggaga aaggtattat aattatcccc atcgtatgga
10501 tgagaaaaca gaaagattgg tgaacatcca agccagaaac acttgataag cagtagagat
10561 agacttcaaa ctcagatgtt ctagagtttc agctcttaac cactatatta tactgcttcc
10621 ttactagaca aacacttagt tttaaagtaa atattttttg agtaaaaaag tgaatgaagt
10681 aaagttaact aaagttaatt tcctgaaatc acagtgatgt ttatgtcttc tttcaataaa
10741 ccattctcgg tataagtggt cagccaggat acatgttgct gtgcctggtg ctccttgtaa
10801 ctcaggaaga tattgcccca ttctaaacag ccagcatggg gtaacagtgt aagcactgtg
10861 aacatttctt ctctgttctt acccatcttc aaacagaaac aaccaggagc agtttaacag
10921 tgatacttga gttatgaact tcatacatat tgcttaattg taatggcaaa ttaatgtgtt
10981 aacatttata gaatggtact gtcattgaag tttgaattta aatgtgggtt ttaacccagt
11041 tctataataa ttgctgtgct ctgggaaaca ctgaatcttt tcaaggagga cagctattta
11101 actataagta gagtcatttg aaatgcagaa gaccagaata tttattcaaa gagtggtagc
11161 aaagaagtga gatgagaact gaggtggagt gttctttcca tcctagtgga gagaacacag
11221 agctRtgagt cagggtatct ggaatctaag catgtctcca ggcttaagtt tctattgtga
11281 gtttagctaa ggcattcctg ttgWtaatac tattgctcta ataccaaaaa gtgtccacta
11341 gaaaatagag taacctttgt gcctttgtaa tgatagtgtt cttttggtat aatactctac
11401 taccacaagt attgcctttt atcatcttaa aaacaaatct gatcatattt ctccccttat
11461 ttattctttt tcctccagtt tttatcgagt gctagctatg tgccaggaat ttataagtcc
11521 tttatgtgtg ataactcatt tataagctca tgcctgtaat cctagtattt tggggggctg
11581 agKtgagagg attgcctgag gtcaggagtt tgagactagc ttgggcaaga tagcaagacc
11641 gcatttttac aaaaaattta aaaattaggt gtggggtaca catctgtatt cccaagagtc
11701 tgaggtggga gaattgcttg agcacaggag tttgaggcta cagggagttg tgatoatgcc
11761 actgcaatcc agcctgggtg acaaagtgag accctgtctg taaaaattat agtaacctat
11821 ttaattatct cagccacctt acgaagtagg cactaattta gacatttcat agatgagaaa
11881 actgaggcac agtaacatac ccaacgtagc atggtaaggg atggagttca tataaaaccc
11941 agMcagttca gcagtattcg ctgtcctgca gcctccgctg ctgataaccc aggcaaacag
12001 ggtctggagt ggacctccag caaactccaa cagacttgca gctgagggtc ctgactgtta
12061 gaaggaaaac taacaaacag aaaggacatc cacaccaaaa ccccatctgt atgtcaccat 12121 catcgaggac caaaggtaga taaaaccaca aagatgggga aaaaacagag cagaaaagct 12181 gaaaattcta aaaatcagag cgcttctccc cctccaaagg accgcagctc ctcaccagca 12241 acggaacaaa gctggacaca gaatgacttt gatgagttga gagaagaagg cttcagagaa 12301 tcagacttct ccgagctaaa ggaggaagtt cgaacccatc gcaaagaagc taaaaacctt 12361 gaaaaaagat tagacaaatg gctaactaga gtaaccagtg tagagaagtc cttaaatgac 12421 ctgatggagc tgaaaaccat ggcatgagaa ctacgtgacg aatgcacaag ctttagtagc 12481 tgattcaatc aactggaaga aagggtatca gtgattgaag atcaaatgaa tgaaatgaag 12541 cgagaagaga agtttagaga aaaaagtaaa aagaaacaaa cacaacctcc aagaaatatg 12601 ggactatgtg aaaagaccaa atctatgtct gattggtgta cctgaaagtg atggggagaa 12661 tggaaccaag ttggaaaaca ctctgcagga tattatccag gagaacttcc ccaacctagc 12721 aaggcaggcc aacattcaaa ttcaggaaat acagacaatg ccacaaagat actcctcgag 12781 aagagcaact ccaagacaca taattgccag attcaccaaa gttgaaatga aggaaaaaat 12841 gttaagggca gccagagaga aaggtcaggt tatccacaaa gtgaagccca tcagactaac 12901 agtggatttc tcggcagaaa ctccgcaaac cagaagagag tgggggccaa tattcaacat 12961 tcttaaagaa aagaattttc aacccagaat ttcatatcca gccaaactaa gcttcataag 13021 . tgaaggagaa ataaaatcct ttagagacaa gcaaatgctg agagattttg tcaccaccag 13081 gcctgcccta caagagctcc tgaaggaagc actaaacatg gaaaggaaca accagtacca 13141 gccactgcaa aaacatgcca aattgtaaag accatcgatg ctaggaagaa actgcatcaa 13201 ctaatgagta aaataaccag ctaacatcat aatgacagga tcaaattcac acataacaat 13261 attaacctta aatg.taaatg ggctaaatgc tccaattaaa agacacagtc tggcaaattg 13321 gataaagagt caagacccat cagtgtgctg tattcaggag acccatatca tgtgcggaga 13381 cacacatagg cttaaaataa agggatggag aaagatctac caagcaagtg gaaaacaaaa 13441 aaaaggcagg ggttgcaatc ctagtctctg ataaaacaga ctttaaacca acaaagatga 13501 aaagagacaa ggccattaca taatggtaaa gggatcaatt caacaagaat agctaactat 13561 cctaaatata tatgcactca atacaggagc acccagattc ataaagcaag tccttaggga 13621 cctacaaaga gacttagact cccacacaat aataatggga gactttaaca ccccactgtc 13681 aacattagac agatcaacga gacaaagtta acaaggatat ccaggaattg aactcagctc 13741 tgcaccaagc ggacataata gacatctaca gaactctcca ccccaaatca acagaatata 13801 cattcttctc agcatcacat cRcacttatt ccaaaattga ccacatactt ggaagtaaag 13861 cactcctcag caaatgtaaa ataacagaaa ttataacaaa cttgtctcag accacagtgc 13921 aatcaaacta gaactcagga ttaagaaact cactcaaaac cacttgacta catggaaact 13981 gaacaacctg ctcctgaatg actactgggt acataacaaa atgaaggcag aaataaagat 14041 gttatttgaa accaatgaga acaaagacac aacataccag aatctctggg acacatttaa 14101 agtagtgtgt agagggaaat ttatagcact aaatgcccac aagagaaagc aggaaagatc 14161 taaaattgac gccctaacat cacaattaaa agaactagag aagcaagaga aaacaaattc 14221 aaaagctagc agaaggcaag aaataactaa gatcagagca gaatggaagg aaatagagat 14281 acaaaaaacc cttcaaaaaa atcaatgaat ccaggagctg ggtttttgaa aagatcaaca 14341 aaattgatag accgct'agca agactaataa gaaaagagag aagaatcaaa tagatgcaat 14401 aaaaaatgat aaaggagata tcgccaccRa tcctacagaa atacaaacta ccatcagaga 14461 atactataaa cacctctatg caaataaact agaaaatcta gaagaaatgg ataaattcct 14521 ggacacatac accctcccaa gactaaacta ggaagaagtt gaatccctga atagaccaat 14581 aacaggctct gaaattgagg caataattaa tagcctacca accaaaaaaa agcccagggc 14641 cagatggatt cacagccgaa ttctaccaga ggtacaagca ggagctggta ccattccttc 14701 tgaaactatt ccaatcaata gaaaaagagg gaatcctccc taactcattt tatgaggcca 14761 ggatcatcgt gataccaaag cctggcagag acacaaaaaa agagaatttt agaccaatat 14821 ccctgatcaa catcgatgca aaaatcctca ataaaatact ggcaaaccaa atccagcagc 14881 acatcaaaaa gcttatccac cataatcaag tgggcttcat ccctgggatg taaggctggt 14941 tcaacatacg caaatcaata aacatcatcc ggcatataaa cagaaccaaa gacaaaaacc 15001 acatgattat ctcaatagat gcagaaaagg cctttgacaa aattcaacag cccttcatgc 15061 taaaaactct caataaatta ggtattgatg ggacgtatct caaaataata agagttattt 15121 atgacaaacc cacagccaat atcataccga atgggcaaaa tctggaagca ttccctttga 15181 aaactggcac aagatggatg ccctctctca ccactcctat tcaacatagt gttggaagtt 15241 ctggccaggg caatcaggca ggagaaagaa ataaagggta ttcaattagg aaaagaggaa 15301 gtcaaattgt ccctgtttgt agatgacatg attgtatatt tagaaaaccc catcatctca 15361 gcccaaaatc tccttaagct aataagcaac ttcagcaaaa tctcaggata caaaatcaat 15421 gtccagacat cacaagcatt cttatatacc aataatagac aaacagccaa atcatgagtg 15481 aactcccatt cgcaattgct tcaaagagaa taaaatacct aggaatccaa cttacaaggg 15541 atgtgaagga cctcttcaag gagaactaga aaccactgct caacgaaata aaagaggaca 15601 caaacaaatg gaagaacatt ccatgctcat gggtaggaag aatcaatatc gtgaaaatgg 15661 ccataccgcc gaaggtaatt tatagattca atgccatccc catcaagcta ccaatgactt 15721 tcttcacaga attggaaaaa actactttaa agttcctatg gaaccaaaaa agagcccaca 15781 ttgc.caagtc aataataagc caaaagaaca aagctggagg catcatgcta cctgacttca 15841 aactatacta caaggctaca gtaaccaaaa cagcatggta ctggtaccaa aacagatata 15901 gatcaatgga acagaacaga gccctcagaa ataataccac acatctacaa ccatctgatc 15961 tttgacaaac ctgacaaaaa caagaaatgg ggaaaggatt ccctatttaa taaatggtgc 16021 tgggaaaact ggctagccat atgtagaaag ctgaaactgg atcccttcct tacaccttat 16081 acaaaaatta attcaagatg gattaaagac ttaaatgtta gacctaaaac cataaaaacc 16141 ctagaagaaa acctaggcaa taccattcag gacataggca tgggcaagga cttcatgtct 16201 aaaacaccaa aagcaatggc aacaaaagcc aaatttgaca gatgggatct aattaaagag 16261 cttctgcaca gcaaaagaaa ctaccatcag agtgaacagg caacctacag aatgggagaa 16321 aatttttgca atcaactcat ctgacaaagg gctaatatct agaatctaca aagaactcaa 16381 atttacaaga aaaaaacctc atcaacaaat gggcaaagaa tatgaacaga cacttctcaa 16441 aagaaaacat ttatgcagcc aaaagacaca tgaagaaatg ctcatcatca ctggccatca 16501 gataaatgca catcaaaacc acaatgagat accatctcac accagttaga attgcgatca 16561 ttaaaaagtc aggaaacaac aggtgctgga gaggatgtgg agaaacagga acacttttac 16621 actgttggtg ggactgtaaa ctagttcaac cgttgtggaa gacagtgtgg cgattcctca 16681 gggatctaga actagaaata ccttttgacc cagccattcc attactgggt atatacccaa 16741 aggattataa atcatactgc tataaagaca catgcacacg tatgtgtatt gtggcactat 16801 tcacaatagc aaagacctgg aaccaaccca aatgtccatc aatgatagac tggattaaga 16861 aaatgtggca catatacacc atggaatact atgcagccat aaaaaatgat gagttcatgt 169-21 cctttgtcgg gatatggatg aagctggaaa ccatcattct cagcaaacta tcgcaaggac 16981 aaaaaaccaa acaccgcatg ttgtcactca taggtgggaa ttgaacaatg agaacacttg 17041 gacacaggaa ggggaacatc acacaccagg acctgttgtg gggttgggag aggggggagg 17101 gatagcatta ggagatatac ctaatgtaaa tgacgagtta atgggtgcag cacaccaaca 17161 tggcacatgt atacatgtgt aacaaacctg cacgttgtac aSatgtaccc tagaacataa 17221 agtataaaaa atatatataa atagtcaaaa aaaagtgttt tgaagtgaat gaaaataaaa 17281 atgtgacatc agaattaaga ctaggccact aaaScagtaa ctagggtgaa atctacagca 17341 tgaaagacct atattcgaaa agaagaaagg tctgaaatca gtgagttcat ctactttatg 17401 aaattagaaa aagagcctgg gcaacatagc aagtccccat ctctacaaaa aatttaaaaa 17461 tgagccaggc atggtggtag atgcctgtag tcccagctac ttgggacatc ggggcaggag 17521 aattgcttgg tcccaggaaa ttgaggctac agtgagctaa tattacgcca cggcactctg 17581 gctaagcaac agagaaagac cttgtctcta aaataaataa ataaacagaa aaataggaaa 17641 aaaaatcaga aaaagaacac atattcaaag taagcagaat aaagRagata ataaaggtaa 17701 aagcagaaat catgagactt cagaaaacaa aaacaacaga aaaaaaccag taagtccMaa 17761 agctgtttct ttgaaaatat cagtaaaatt aagaaacctc tactagacta attgggaacM 17821 atagagaaaa gaaacaagtt aacaatgcca ggaataagat aggtgactat aaacacatta 17881 aaagaatgat aagaaaatat tatgaacaac tttatgctta aacatttcac aacttggatc 17941 aaataaattt cttgaaagac acaactaccM aagctcactc aagaagaaat aagtggcctg 18001 aatagcctaa catctacttt ttaaaaataa tttatagtta aaaaaaaaac tgcccacaaa 18061 gaaaactcca ggcccagatc actggtgaat tctaccaaac atttaagaaa aaaaagacac 18121 caattctaac gaaactcttc caacaaagtg aaaagaaaga attatttccc aaagtattct 18181 atgaggccag aattaccctg atatcaaaac cagaggcatt acaagaaatc tacaggccaa 18241 tttccctYgt gaacatagat gcaaaaattt caaacaaaat cttagcaagt caatcataaa 18301 aaagaccaag ttctggctgt ttgtgtgtat aggaagaaaa caaaaacaaa aaccaaccga 18361 ggtttatccc aggaatgcaa ggttggttta acatatgaaa atcagttaat gtaattcatt 18421 ataataacgc aatatcacca tattacattc tcaacagatg cagaaaaagc acttgacaaa 18481 attcaacatc catttatgat aaaaaaaact tttggaatag gaaggaactt cctgagacag 18541 acaacagcaa aacaacagag gWaaatcaat aaaccaaaag ctggttcaat taagccaaaa 18601 attaggaata gagggagttt cccaatctga taaacaatat ccatgaaaaa gttataggta 18661 atattatatt attggtagaa accagacaaa aatacctgct ctcaccagct cttttaactt 18721 taatacagag tctcattcta tctcccgggc tggagtacag tggcacaatc tcagctcact 18781 gcaacctcca cctcccaggt tcaagcaatt ctcgtgcctt agcctcccgt gtagctggga 18841 ctacaggcac gagccaccac accacccccg gctaattttt gtatttttag aagagaagga 18901 gttttaccac gttggccagg ctggtctcaa actcctggcc tcatgtgatc cacccacctc 18961 aacctcccaa agtgctggga ttacaggcgt gagccaccgt gcccggcctg ctctcaccac 19021 ttctattcaa cactgtattg gaggttctag cagtgcaacc agggatgaaa aaaagaactt 19081 aaaaaaaatt aggaagaagt aaagctgtct tcatatcttt atttgcagag gacattaaca 19141 tttatgtcag ggatcagcaa actacaatcc aaaggccaaa tataactgtt gcctggtttt 19201 ttgttatttg cttttgcaaa taaaatttta ttggaacaca gccacatcca tccatttact 19261 tatcaactat ggctgctgta aacccaaaag atctgagaca ggtctcaatc aattgagaac 19321 tttattttgc caaggttaag gacatgcccg gaagaataaa acatggaatc acataaatgg 19381 tcagtggtct gtgccttttt tcaaaaatga ttttgagggc tgcaatattt aaagggaaaa 19441 agtgggctgg aggggaaaga gggagaatgt ggtaatccac atgttgcaag agaaaaggag 19501 caggtaggga aatagtcaat tatgtattca tattgtgccc agtaaataaa tactttacat 19561 aagataaggt gaacatggag tagctacctg gggagatact gaaccttttc tctatagctg 19621 tctgcttaga aacaaatgga aaggcagctt cttgtaggac acagctttca gcttagtttt 19681 ttccttttgg cagagtgaag tggggtctga gtttttattt tcccttcaca ttgctttcac 19741 tctaaaatgg cagagttgag taactgtagc agagatctta tgaatggaaa ataaatacaa 19801 gatgcttaac ctaatttgca tcatttaatt tgcaccttaa tgcaaattaa aaccacagta 19861 agataccact gtgcatctat cagaatgaca aaaactaaaa agattgacta tacctagtgt 19921 tggtgagggt atcaaaaaca agatttccca tccattgcag gtgggaatgt aaaatggtaa 19981 gaccaatttg gaaaagtgtg attaaaaatg aaaagataga cctattattt gattcagtta 20041 ttatactccc acatacttat gtaagagaat gaaatcatat gtttatacaa agatttgtac 20101 acaaatattt atttgtagca tctaaaaact ggaaataacc caaatgccca tcaatagtaa 20161 ataaacatct ggtggtattt ccataaatgg aatatgacta ttcagcaata aaaactaata 20221 aattattgat gcatgctgcc acataaatga atctcaaaat aattatgctg agtgaaaaag 20281 gccagacaag aagagtacat atggtatgat tccatttatt taaaattcta ggaaatccaa 20341 actagtgaca aaatcgctaa gtggttgcct ggggacaggg cagatggggt gaataaggaa 20401 ggaagggcag aagggaagga ttacaggagc attaggaaac ttttgagggt ggtgggtagg 20461 tcggctagaa acctgggagt tgctggatat gttcgctatt gtggttgtgg tggtggtggt 20521 ttcaaagctt tacgtatatg ctaaatttta tcgaattata tggtataatt taaatatgtg 20581 cactttatat gtcaattata ccttgattgt ttttttaaaa gtccaaaacc aaaaaattgg 20641 gctctgatgt gtggccatgt gaatgagtga tctggtgaaa aaggatacac tagctctgta 20701 ccaaatccct gactcccaaa actggacata gatatggagg tattctctct ctccctctgt 20761 gagaagctta gggtataaga atatatggct catatgaatc cttgaattaa ttctttgttc 20821 tcactctacc tttcctatgt ctacactaca tgcctgtacc atacccaaca cacatccatc 20881 tttttttttt tttttttttt ttttttttga gatggagtct cactctgtcg cccaggctcg 20941 agtgcagtgg cgcgatctcg gctcactgca agcttcgcct cctgggttca cgccattctc 21001 ctgcctcagc ctcccaagta gctgggacta caggcccgcc accacgccct gctaattttt 21061 tttgtttttt agtagagaca gggtttcacc atgttagcca gcatggtctc aatctcctga 21121 cctcgtgttc cgcccacctc ggcctcccac cattcatctt ttaactatgg aaaaatacca 21181 gtgaaaatca aggcattcat tttgtgccat tatgcacaat cataagggaa aagaaaagag 21241 tcagtaaaat cctagtRtta aagaggaaga aactctactt ctcctcatac cattaattcg 21301 atgttaggga aggatcattg catggtatga caagaatcag agtttctcag cattcagttt 21361 ttggccctgt ttttgaaatt ctgtagaatg agaccctaca atgtaccaag gaataaggaa 21421 gaaaatggga aagcaggaaa atggttaaaa tcacaaggta aaagaaagct gaagcttaga 21481 aaaaaaRttt gctttcattc catataaaca ataaaaaggg aatctccatt ttcttttaga 21541 taatgcctct caattaacaa aaatttcatt ttcagcttct gtagatccat aaattaataa 21601 gatacctaca catcaaatgt ccctcagtta taatggaagc ccagtgtgct cacaggatcc 21661 aaacacctca gcgatctgaa gtccagcctc tccacctacc acaacctcca taatcacact 21721 aattacattt ttaaaatcaa ggaagacagt ggatctctaa acgtgaaaaa agaaatacct 21781 tctactatgt gaaaactgcc catctcatat ggcaacactt ttattacggt aaagcaattt 21841 atttatttat tgagacagag cctcgctctg ttgcccagta tggagtgtgg ttgcatggcc 21901 ttggctcact gcaacctctg cctcccgggt tcaaggaatt ctcctgcctc agcctcccaa 21961 gtagctggaa ttacagggat gcaccaccac gcccagctca ttttttgtgt ttttagtaga 22021 gacggggttt aaccatgttg accaggctgg tcttgaactc ctgacctcag gtgatccacc 22081 tgtctcagcc tcccaaagtg ctgggattac aggcgtgaga caccgcaccc agccagtcaa 22141 tcaatttaaa atgggctgta tacttggcat cagagatgga agtgcaggtt tcaagtcact 22201 ctatctaaat gaaactacta tgtgtgtgtg ttcattatat aaattatata tgctgacaca 22261 gatatactta attatgtacg atgacacaca cacatatttt gctaacattt cctgcagtat 22321 atactatgtg tccaccacca agttccttac caacactgaa cttttattaa catattcctc 22381 ccaacaatcc tatgaggtag gtactgcaat taccccaatt atagacaagg aaactcactc 22441 acacagaggt cacatggcta ctaattggta ccggtggagg agatcaactc aagtagcaac 22501 tccagagtac atgctcttac tcgttttgcc ttgcccaaac ttaagatagc tgccttcatg 22561 tcgtgtaaga ggatatgacc tccaatatga atggagaggg gtagagcatg aaatagagca 22621 tgaaaatctt gtctcatttg aggtctgaag ccacagacca aagagaaagt tMtatatgct 22681 gtcttctgtg tgatgggcta gggcctggag tgggctccaa gtaataccca gcaaggtcat 22741 caacagagca ctccagtcca gtgcactctg ggagagggat cacttgaacc caagggctag 22801 catctcggtc acttgaatct tcagtaccta gcatggtaaY tggcatatat taagggatca 22861 taaatgtttt actcaatgaa tttcaacMaa tgaatctttt acagagtagt aattgacaat 22921 gtgggttctg ggactggact gtctggtttt tttttttttt aatactttaa gggtacatgt 22981 gcacaacgtg caggtttgtt acatatgtat acatgtgctg tgttggtgtg ctgcacccat 23041 taactcgtca tttacattag gtatatctcc taatgctaac cctccccgct tcccctaccc 23101 cacgacaggc cctggtgtgt gatgttcccc ttcctgtgtc caagtgttct cattgttcaa 23161 ttcccacctc tgagtgagaa cacgcggtgt ttggtttttt gtccttgtga tagtttgctg 23221 agaatgatgg tttccagctt catccatatc ccaacaaagg acatgaactc atcatctttt 23281 atggctgcat agtattccat ggtgtatatg tgccacattt tcttaatcca gtctatcatt 23341 aatggacatt tgggttggtt ccaagtcttt gctattgtga atagtgccgc aataaacatc 23401 cgtgtgcatg tgtctttata gcagcatgat ttataatcct ttgggtatat acccagtaat 23461 gggatggctg ggtcaaaagg tatttctagt tctagatccc tgaggaatcg ccacactgtc 23521 ttccacaacg gttgaactag tttacagtcc caccaacagt gtaaaagtgt tcctgtttct 23581 ccacatcctc tccagcacct gttgtttcct gactttttaa tgatcgcaat tctaactggt 23641 gtgagatggt atctcattgt ggttttgatg tgcatttgtc tgatggccag tgatgatgag 23701 catttcttca tgtgtctttt ggctgcataa atgtcttctt ttcagaagtg tctgttcata 23761 tcctttgccc acttgttgat gggtttgttt gtttttttct tgtaaatttg tttgagttca 23821 ttgtagattc tggatattag ccctttgtca gatgagtagg ttgcaaaaat tttctcccat 23881 tctgtaggtt gcctgttcac tgtgatggta gtttcttttg ctgtgcagaa gctctttagt 23941 tttaattaga tcccatttgt caattttggc ttttgttgct attgcttttg atgttttaga 24001 catgaagtcc ttgcccatgc ctatgtcctg aatggttttc ttctagggtt tttatggttt 24061 taggtctaac atttaagtct ttaatccatc ttgaattaat ttttgtataa ggtgtgagga 24121 acggatccag tttcagcttt ctacatatgg ctagccagtt ttcccagcac catttattaa 24181 atagggaatc ctttccccat ttcttgtttt tgtcaggttt gtcaaagatc agatggttgt 24241 agatgtgtgg tattatttct gagggctctg ttgtgttcca ttggtctcta tctctctttt 24301 ggtaccagga ccaagctaaa acacttttta atttcccttt tgattcttct ttgacttaca 24361 gattattgag aaatgtgtta gtttccaaac attaggattt ttcagatgcc tttctgtact 24421 gatctctatt ttaatttcac tgtggtcaga aaacaaactt tgtatgactt gaatctttta 24481 aaacgtattg tgatttgttt tatggctcgg aatatggtct accttggtaa atgtcccatt 24541 tgcacttgtc aaaaatatgt attctgctgt ctttgggtta ggtgttctat aaatgtgaag 24601 taggtcaagt tagtcgatag aatttttaaa aatcagcatt cttactgatt ttctgtccac 24661 ttgttctgct attgagagWa gtgttgaaat ctcgaacaaa gaatcctatg gatttgttta 24721 tttctccttg cagttctttt cagtttttgc ataatatatt ttgaagctct gttactaggt 24781 acatacatgt ttagaatttt tatgttcttt tgatgaattg acctctttat cgatttttaa 24841 atttattgtt atgaaatgac ttcctttatc attatgaact aacctttttt atccctggta 24901 atattctttg ctctgaaata tactttgata ctaatatagc cactccactt ttcttttggc 24961 tagtgttaac ttagtatatc ttttttcatc cttttatttt gaagctttgt atctttatat 25021 ttaaagttgc cttgcttttt taggagaaaa tctaatctaa caatatatgc catttaattg 25081 ggatgtttag actatttaca ttgcgtttac tgatatagtt aggtttaagt cttttctctt 25141 gcatttgttt tcctttgttt catctgttct ttgttctttt gttcttttta tgccttcttt 25201 tctattgggt aactttgttt ggtctttttt gttggtttat tagctataac ttatgacctt 25261 gatatattta aagagtagtg gttaggtgtt ttgcagaaag tcttcaattt gagtttatct 25321 gatgttttct catgattaga cttggtttaY agattggggg agggggtcag gtgctgtgcK 25381 gctaatgcct gtaatcccag cacttttgga gaatgaggtg ggaagattac ttgagcccag 25441 gagtttgaga ccagcctggg taacatgtga gatcctgtct cttaaaaaaa aaaattagca 25501 aggcatagtg gcacacacct gtagtcccag ctactcagaa agccaaggtg ggaagatggt 25561 ttgagtccag gagtttgagg ctgcagtaag ccatgattgc gccactacac tcaagcctgg 25621 gtgacagaat gagaccRtgt ctcaaaataa ataaataaaa aattggagga ggggatagat 25681 accataaaag tgcagtgccc ttctcatcat atcaaagggt atataatatc atcatgacat 25741 gttgctggta ttgctaccct gatcactagg tttaggtggt gtctacaagg tttctccact 25801 gtaaagttag tatgtttctt tttccctttc tattttttag gagttcagcc cacactcaag 25861 gagatggaaa ttaagcttca cttcctggag ggaggagaat cagataattt gtggatgtat 25921 gctaaaatta ccataataat taataaatat ttcggtggat aagatgttat gaggctattc 25981 aagtattctg ataaagtttt ttcatgaata ttggcagtca ttagtggatg ttccctatag 26041 cagttattac tatggtgttc taatgattat ttcctatttc ccttattctt tctacatttg 26101 ttcattgtaa ttctcctgta aagaaaattt ggcctctgtc tctcatttat ttgcttattc 26161 atcatttatg catattagtg tggatttatg ggtattgtat tctttgtatt ataatccaat 26221 agtatcataa ttttttttct taaaatcttc cagctttggc cactggaagc tctttcaggt 26281 ggctgggatt ttttgtcgtt gtttgttttt tagcactttc ttgctctctg acactacagg 26341 gtgctccagg cttatcttgt attttccctg tcctagaatc agccattttt cagggagccc 26401 tgattctttt tactagagaa tggtatatgg gaaactagac ctgggtacta gaaatgcttt 26461 tggttatcag gtgtcactgc ttctaggctc tcggtgaaca gggctaggag atacatgtat 26521 ttatatccac gtatacacac atatctatac tatttctcta tttgtatcta tgttaaaata 26581 aacattagtt tatagtggta tgtctgactc aaattcagca ctacagggtt aattctagca 26641 ttttcccctt gcatattcat tctttgatgt gagaaacttg gctgtcatta cctacaacgt 26701 atttatagtt atttgtttaa ccaagacaag gatatctgct cacaccattt ctactcaaca 26761 ccatattgga ggttctagcc aatacaacca ggcatgaaaa agaaacttaa aaaaaaaaMa 26821 atccacggcc aggcacagtg gctcatgcct gtaatcccag cactttggga ggctgaggtg 26881 ggtggatcat ctgaggtcag gagtttgaga ccagcctggc tgagatggtg aaaccctgtc 26941 tctactaaaa gtacaaaaaa taaaataaaa taaaataacc ggggatggtg gtgggcacct 27001 gtaatcccag ctactcagga ggctgaggca gaagaattgc ttgaacccgg gaggcggagg 27061 ttgcactgag ctgagatcac accattgcac tccagcctgg gcgacaagag caaaaaactc 27121 catctcaaaa aaagaaaaac aatccaaatt ggcggggaag aagtaaagct gtcttgatgt 27181 cttcccttat tagagagaaa tatttggtag tttgcagtct gttctacctc aggagagact 27241 gcagtaaaac tgatgtgaac atctcagaat tcaactgttt tgtaaatacc accacccctt 27301 ccactttcct tcagcatccc agttaccact ggaatatttt atagcatatt tatttttcct 27361 ttaacatgtt aaacctaaag cccaggtttt gcagcttgaa tatgtttcct cttgttgata 27421 tgacagtggg atatgacagt ggagtRaaac aaagcaaacg aataactggt ccctgtcttc 27481 catgcaatgg tctttgatag gtgagattcc cttatatttt caagttcggt tcctgaagta 27541 gctcatccat tcagattctg aagaagggtg atcactcctg cactgtcttc aagataacag 27601 tgaaggctag ttccatgtac ctccgaaggg tactgtgagc cctaaagagg ctatactgga 27661 tgggggttaa tgcagaatgt ctgcagttgg aatgcaggtg taactttgaa ttattttttt 27721 gtagcatatg agagcacaca ttcctctctt ggaattctga taccagaatc tgtaatcaca 27781 tgcaaatagt gtttatggga ctgggctcac acagaaagta gcttcatgcc aaggtcttat 27841 gctcagggca aatattaggg ataatgtatt tccaaatgag ttattttact gaacaagaat 27901 atgccttgac acatgttctg tgtttagtca tacacatttg tcatgcctca gtacctctaa 27961 gatgggtctc atggcaacag gcaggaggat tttatgtgtt catctgtgct ttagagccaa 28021 gaatatttta taattggtct gactggtctg cagttagaaa aagtaacagt gccaggctca 28081 tgcaaagatg gtgaccctcc ctccacccat catttttata tttccattat ttcagtgtag 28141 aactgagccc atggtgagtc aagccaagtg ctgtacagtt ccatctcctt tgcacaggtt 28201 ctccctgagt tactgaaaac actgggaagt cattgcacca ctacaatgga agtggtgctg 28261 tccttcaggc tattctcact tcctcctctc tgtcctctct ttgtctctgg cctccccctg 28321 cctgtctccc accaacccca accttgtctg ctctgctgcc ttcaccctgc ctttactctt 28381 cctcctctct ccctgccacc actccttctt tgatatttta tccaYgtttt tacttggtgg 28441 tttgatggtg aaagtgtata taagacccag ccattccctt tctcttttgt ctttcctYat 28501 agcttcgatt aacatggcaa taatttcctc cccatgataa tcctggactt tccacttatg 28561 ctttaaaagc ctttcaattg gagatgagag cggggttggt taacggtctt tccaggttag 28621 ccgtttcttc Raacacttct atttaaggtg gctgctaggt ggagttttac tctggtgtta 28681 tctgactgga ttaatcagat ttgtgctcaa gaaatctttt tttttttttt ttgatacaga 28741 gtcttgcttt tgttgctcag gctggaatgc agtggcatga tctcaactca ctgcagcctt 28801 tacctcctgg gttcaagcaa gcacatcggc taatttttgt attttttttt ttagtagaga 28861 cagRgttccg ccatgttggc caggctggtc tcaaactcct gatctcaagt gatctgccct 28921 cctcagtctc ccaaagtgct gggatcacag gcatgagtca gcacgactgg caagaagtct 28981 ttggtggttt gggtttggaa atgatagaat caaatagaaa gaaaagattg tacactcaaa 29041 gaaatagcac cKgctttgag gtcttcctgc tggcttaacc ctggggtgtt acatggtact 29101 gctctgactc agcttttagg atcgccactg gaagagcttt tcatataaYg tctatacagt 29161 gaaatgactt aagatttttc ttttaagaag tgccattgcc agagtattag tgtttaggat 29221 tctccagaga aacagaacta ttaggagata gagagataga gatgtattat aaggaattga 29281 ctcacacgat taagaaagct ggtgagttca gaatctgcag tatgggctag caggctggag 29341 acccaggagR gtcgacgatg ccagtgaagt ctgcagggca gtctgctgga ggagtttcct 29401 cttggagagg aggccagtct ttgtgtttta tttaaatgat caggtgaggc ccgccctcat 29461 tatggagggc agtctgcttt actgaaagtt cacaaattta aatgttaatc tcatctWaaa 29521 aaccctccaa gttgacacat aaaattaacc ctcacagcta ggtttatttg gaattccatg 29581 atacttggtt gggtcttttt gtactttcat tttcaatggt ctgttttgta tataaccatt 29641 tagttaagcc aggtcacaaa atataaccag gacttcagct ggctgtgctc ttgtcagaac 29701 ccatatttga tcgatttact atatactttg ctgtgtgtat gggtgagtgg gtctgtaaaa 29761 tgctgatatg tgttctttat ccacccccac ctcccacccc ccattttctc ttaaattcct 29821 taaaaaattg gtgaaggaac taagacattg ctgcaaagat gctattttat gggatatatt 29881 cttctctagg gtagactctg cgaagcagct gtacagcagc ggtctgctgt gaagggtaaa 29941 catgtagcct ttctccacta gaaagcctgc gctgccgttg tatgtttagg cttgagcctc 30001 agcggctagt gcagcctcgc agtaatgcat gctgacgtga gtcctggtca aggaaaaatg 30061 tcttttgagc aagagacaaa ctggacagaa tgccagcaag ctaaatataa ctccttagag 30121 agccagacat gcataaacta gttcttagtg agcaacacat cagattcaga gtggaaacat 30181 tttctaaaag aacttctttt ccttcttggc ttactgtaat gagtataaac ccaaattaat 30241 ttaatttttc gtggaaagct aagacaacca ttgatttttc tctcagcatc tctgcagtta 30301 aatgttagta gccattgtta atgttggaag cctcccgtct cccagtgccc cagcattctg 30361 ttcatgagtc aacaagtctg ctctgctatt ccgatgagtt tcactaccac aaggaaagag 30421 gacggggagt ttgcacattc aaaccttgac gaatctggga gttcagtttt tcatggaccc 30481 tcactctgct tcaggatgct cacgccatta tgtgaaaact cttagcaaaa tccatcaaaa 30541 tgagccaggc tctatggaat caggaaattt gtgggctgag actaacatta cctcaatatc 30601 tgttatggct taaatgtatt gttttaataa tattttatgt tagttctttt tgcctgaagc 30661 tgtagaagca ttttatagcc agtaactctt cagaatagta caggaaagca ggcgtcatcc 30721 tcatgctcaa caatagagct gtgtttctgt gcaccaaccg cagatgctgg gtctggcccc 30781 cacagaagga gtgcctcagt agggcatccc aaatggggct gtgcatagcg tcactccaga 30841 ggctggggac agtgcagatc cagacctttc ccagcctgtt gagttagaac tgggggactg 30901 tgctccaggg cgtgaggagg tgggttggga aatggtattt taaacaagcg aagtgattgt 30961 catataactt gtctggcatc agatccgcag actctggcag agtttccRga aggtctctca 31021 aaactgaaat aagaaactgc agttgttgtc cacagctgct aaaattgttc caaattagga 31081 aagggaaggg gaaacagaac acagtagttc cagagaagaa tctgaggagc cgtgtgagct 31141 aggaaggccM ttgtgaRcag aagcgagtca gtatttcctc atttcctctc ctgccttatc 31201 attcccatct gccagctccc tccttttttt ttttgagcat gtaaaacatg ttttgttgtt 31261 ctttaaaagg gttcagggtt tggttttaaa tcaggctgca cacctttcaa atcattctga 31321 catctctcta tgtcaaactg gcttcagcta gcaatacttc attaaatccM aaagaaaaag 31381 attcctttaa ttttaaggaa aaaaatccag ttttgagaac aattaacatt agtctttaat 31441 ttaaaagaaa atgagggcta atgtttcatg ttgctttata catccttctc ctcaatacag 31501 aaccaggaat gtaattttcc taacttgggc aggcactgat actgatggac actgcgtgca 31561 tgtgtgcgca cacacacaca cacacacaca cacacacacc ctcctcccaa acaaaattca 31621 gagtgtgtca aagggaaaag gtttgtcatg gtattgatca aaacacttgg agtcaacact 31681 gtctacctta gcttagcacg tgtgctgttt tgatcttgag tctatattaa tggaatctgt 31741 tgttgtgttt tgaaatggag aattttggat ataccattat ttttttactt ttaagtccca 31801 gaaacaattt cagaaagcat tattttgttt tgttttctgg gatgggagaa gagagaatct 31861 acagatttgt atttagttaa aaatcaaaac tccagcaata ggaaataggc agttcacata 31921 gccggccaac atttgaggta tcatcagtgt gagacaaggt cccggtctgt tcctactggc 31981 ctagctgacg ctaacaaaat gggaggctta tggctctgca cagcaaattc cagcaagata 32041 agatgacttt aagcctgtat tcagagcctt agaagcaggg cactactgag aatgtaatat 32101 ttagaggtaa aaatagtgca agagcccctg atggtacccc tctacctgct ttgtccatgc 32161 agcctgatcg taactgcctt ccacaaagga tcagaaaaac acctcttctg gatcagtcca 32221 ctaaacccag ccaagaatct gcagaaatgc tgctacaccg cttcaccagc aacacagtcc 32281 ctgcgctact cactgcgttg cccaacgttg cttttctacc ctgttatggc ctgtggacac 32341 atactcacta ggcatcatcg ctggttttaa accagggata ttttttaatc tcaaaagaac 32401 ctagtaataa caaggcaatc aatggttaaa ctgaacttta catatagggg gcagtttgga 32461 aaacaccaca agcatttctc aagtcgaaaa tatgtgaatt cgctgctatt tctttgaccg 32521 tttctgaaca ttcacagtac aagtcctaaa ataaaaattt taagctacca gcattctctg 32581 atactagaca gaaaatcaga gtaacgctac agcccacaat tgtacagggt gtagaaagta 32641 ggcccactgc ctggacatct ttgttgatgg cctggagtcc tcccttttag aagacaagtt 32701 tctgggatat ccacttagtt tcactggtta gggaagagaa gaattcaact ctcagctaac 32761 ttctttgaga agagactcct cttaaaatgc catttcccca ctaatttatc aaaataaaac 32821 aaaaggctct tggctacttt gtctagtatt agatagggtt ttgaggaaaa gaagctaaca 32881 tgaacaccct ttcatcagta aagactaaaa accacctgga tcatataata tatctatatg 32941 tcactgcttt tatttattta gttatttgtt tgtttattta tttaaaagtg cccaggtggg 33001 cttaaggctg ccagactgca cgtacatcta cggcaacaag ggcttctatt ccatctacaa 33061 cttgcatcag gggaaaaagg gacatgtagg agaggaagga aaaaaggRga aaaatacacc 33121 accacccccc cccccccaaa aaagggaaaa agatcccacc acagggagat ctatgtgcca 33181 agcataatga agagtgtgct ccccagacag atggttctgc acaggctaat gttctgctgg 33241 ttttccttag agacctattt tgaaaaagtt taaaaagaca gatttcaaaa taattcaatc 33301 ctggcagaaa ttcaaactcc aaaactagga gcaaaatcat ccttcactga attaattcct 33361 tttytctttc tcttttctta aacattttat tcattttata gagagatttc ttttttgttt 33421 gcttttgttc caatcatgag gagagtggtt gaggagtagt gaatccatca ctactttgat 33481 gacacaggta agattccaga ttcacatttc caggtaccaa gggttccctc taaatgccac 33541 aatcaagtga gccttcattt acatttcttt ctactgaaca cagcagtgcc cgttggtatc 33601 tctgaagcgt aatcacatct taggtcagta tttctccaag taaagcagct gtttggaatt 33661 gaaggccccc cttctttttt gtgttataaa ctgaactgca tcttcgtact tcattccata 33721 ttcaatcaaa gcaagtgcaa ccagcacagg tgcccttccc aatcccgcaa cacaaggcac 33781 tgcaacacag ccacctggct cttcacagaa tttggtttat aacaggttta accaatcatc 33841 tactatctga ttagggggtg gagctccatc atcaaatggc caatctagaa ggtggattcc 33901 ttctttttca actggagctt tatcatacgt agcatcacaa actcgaacca aagtcatcac 33961 tccatacttc ttaagttcct ctgggaactc gttgagagta gcactggtag ggttgtgagt 34021 tacaagaaaa cgcaKgttct cacaggaaat ctccacaggg gctggacggt tcattatggc 34081 aaatgaaaaa tgtgagtgtg cgtgtgagtg tgatgggaaa agtgaaaRaa atcaataaat 34141 caaaatgttt cacagcagaa aacattaaaa agaccactaa aatgcctatt atcaatcagt 34201 gttttctcta ttcaacttgt ttattcctta tgaagcttct gtcttcaaga taagcaaagt 34261 atttagaatc cacttgaatc caaattcaac ttgggcctca atttctgcag atggatacct 34321 tcggactcca aaaaaatcca actacataag cagcctttac tacatttagg cactagtgag 34381 gcaagttaaa agtgtaggtc gctttccact tgtttccttg atgcctaatt ttactggagt 34441 cattaaaaga aatatgcttg caattaaaaa aaaaagccag ctatttctga ggccggtcgg 34501 tgtgtccagg agtgttcaag gcagtgttaa ttatggcaca tagaacctct aagcttgtca 34561 gcgaaaacgc tgtgctgagc ccggcagaag tctgccctca cactcagaat cgccgtaaat 34621 gtgcaacgta tattccaacg aaaaacgctg gcgagcgggg accgggcatt gaagtccgKc 34681 ggtggcagga gcgaggaggc gcccctctcc tcagttacct ctgcggcagc ggcgacgaca 34741 ccccccagcc tcagcgcgcg acacccgccc ggtggcggcg gcgcgtctcc acgagtctgt 34801 cttgctgctg ctcctgcagc cgccgctgcc ctgtggtgtc gcctcccgag gtgcacggcc 34861 gccgccactg ctgctccagc cgctcccgga cggacaacgg ctacagcccg gccgatcgtg 34921 ccgccgccac cgccgctgca cgggcagcca aaagcagctc ccgccccctc ttctctctct 34981 caacttagtt ccacatcttc cctggaagga agagatggct gtgttgaaga tgacggtgcc 35041 agctgcacat catgtcagag gcttttttta ggtcgcttcc cttgcagact catccaagat 35101 agggttttac tcaaacaatg ggatctcctt ttaggcttca aggtctgatt catgttttcc 35161 ttttcgtaca gatcctcttc ccactttatt tgttgctttc ttttttagtt ctggatagat 35221 tttaatattg tatttcttct tataagatta tacaataaag caagaagtag ggagtaagag 35281 gcaaaactcc cagcttctgg tttttacttt gtacttgtag ccattgacct cgctagcatc 35341 cgatcccctg tctgggacat gagtgtagtc atctttccat cgatggatgc tgggaagctg 35401 aatcggtgtt gagaacgtgc tgtgagacgc agcacagaat gtgagccccg acaatactca 35461 ggagaatgta cagctcgttc aagtcccagc agcactgtgt gctcatcctg tagctccttc 35521 ctgctttgct aattatcaaa atgttggtag gagaaagcac agtgtctgct ttgttttttc 35581 tctacattag acatcagtcc tacaagctgg ttgacaggcc tcttcagaaa aagcaccccg 35641 atgggttcat tttcctcttc agcagaatag tcaactgatc tacaggaagc agcgtacctt 35701 cacacttgct ctatgtcaaa atcatgctct ttgtgaaact aaaggtctca ctttcctctg 35761 tccttctctc tctgcacact gctgattggc tggctcctca tatgtgaaaa ttcgtgttct 35821 gtatagctgg taggttttcc tgcgacatcc cttccctgtc ccacccttgt cttcccagga 35881 aaagcataga ttttccttgt ggacaactgt ccataaaggt agaatgctag taatacttga 35941 gcctagaaat tacttaacca gatgacagta aaacttagaa attgatccca aggtcctctg 36001 gctctctacc ctgcatttgc tttgctgagg ctctctaaac aaatgaggaa aaggctgttg 36061 ttcagaggtg gtgactcata tgcagatgga tggccacagt gcgctccatc cctgccatct 36121 ctctttgtca tcctctgtct tgcccatgct gcagtgcctt gcagatttgt ttatatgcat 36181 gactttgtca tctgttgctg agagctgagg aaaacctcct gactggtaag tgcctgtgct 36241 gtttatctta gggcaaccca catttctaca tgctttaaag caggcttcag aactgggtta 36301 ctttttatac agaagtgcat gttgcctgag Yctctttatt agtgtggttc aaacatgagc 36361 acacagatat gggagggatt ttgctgagga ctgtgagttg gagatgattc aggaagtcta 36421 tgacagagct aggatagcaa agtcagtttc ctggtcaggt cttcctgtgt agaaaactgt 36481 gctagatacc actgtgcatg gcaaggggca tgaatctgga agtaaaactg tcttctgtct 36541 aaactctgaa tcctaatttt gtctccactc taaatcccac tgattattag ctgtttaata 36601 cctagttctg gccttaagga taataataac ttctccttct ctgtcattgt gacagataaa 36661 ctggcactga tgcattgtag gatagctttg cctttgggcc atcttagtgt cacttagcat 36721 ttgagaaata agtggctaga ttatagagaa ggaattatca aactattatc tcttgccaca 36781 tttctggtca agcatgcaca caataaaaac aaaacaccaa agcgtgtagt taatctcttg 36841 catgaagtca taggaagaag aagaattgta ggcatcagaa tgtaatagaa agagtgttag 36901 acatggagtt taagaataca gcttgaagtg ctggctccat cacttggctg tggactatag 36961 gcaagccact tggccacatc atcggttgta aattggaaat gataccactt gctctgactg 37021 tcttgaagag tgaccctaag gaattaatct gaatcatttt cacttccttc cctacttctg 37081 gaactatgat gtctgatggc cctggtttca agaataagaa ctaatgccat tgtagtgagt 37141 cattttaaaa cttgatgcct tttggccggg cgtggtggct cacacctgta atcccagcac 37201 tttgggaggc caaggcaggt ggatcatatg aggtaaggag tttgagacca gcctggccaa 37261 catggtgaaa ccccgtttct attaaaaata caaaaaatta gccggacgtg gtggcacatg 37321 tctgtagtcc cagctactct ggaggctcag gcaggaggat cacttgagcc ccggaggtgg 37381 aggttgcaat gagctgagat tatgccactg cattgcagtc tgagcgacag agtgagactc 37441 gatttcaaaa caaaataaaa cttgatgcct ttgaaaataa atacagaatt agtttaatat 37501 atttYtaaca agtaggcaca acagagaatg gagtgacaac cgctgctcct agaatcacat 37561 taagaaaggc atagaagaga ggaaacagaa gagagtaagt gaggctggtc agtattcagg 37621 ggacctctcc atcatcacct gtgcactctc tgggctcttc ttactcctct ctgcactgga 37681 tgtccagaca gacatgcctt tcttgttggg aaccaagtgc ctctaagaaa gcacatattg 37741 acccccactR tgaatcaaac agtggttttt ttgtttataa aagtgaaatc agtctttggg 37801 gtgttgctta cagtttaaaa aaaaaaggaa aagaaagaaa aaagaaaggt tttcctcttg 37861 ggctttttag ccttggcctg aaactgagct gatatattgt tattatcact tgagaacaca 37921 acagcctgag gtccagtcgt ctccttaatg tagctgggga tgccattcca tgtggtttta 37981 gaaatccttt gttatttctg ctcacctgca gagacagccc tcctgggagt ggaccccaga 38041 gtctgtatca tcagaactca cctctctctg agctgggatt aaatgtcatt gaacaaaata 38101 cttaacagcc tttgtcaact gtgaattcaa gcatattttg gtactcaaaa atttagaatt 38161 tctgtatttg gtataaagtc agatgatatg gtttggcggt gtgtYccacc caaatctcat 38221 ctcaaattgt aatctccatg tgtcaaggag ggggccctgg tggacgtgat tggattatgg 38281 agttggcttc cccccatgct gttctcatga ttgtgagttc tcatgagatc tgatggttta 38341 aaagtgtgtg gcagttcccc ccttattcgc tcactcgtgt gcatgtgtgc tctgtctctc 38401 tcctcctttc ccctgctgct ttgtgaagaa ggtgcctgct tcccctatgc cttccaccat 38461 gattgtaagt ttcctgaggc ctcagccatg tggaactgtg agtcaattaa accttttttc 38521 ttaataaatc acccagtctc tggtagttct ttatagtgtg aaaatggact aatatattgg 38581 aaaatactat ttttacattt tttgtatctt tctcttgctc tgtattcatt tccattcccc 38641 tctgtattag tttactagga gttgcctaac aaaataccat aaagaggtgg cttgaacagc 38701 aaaaatgtat tttcacatag tactgcaggc tgggaagtcc aaaatcaagg tgctggcagg 38761 gttggtttct cctgagacct ctccttggcc agtaggtgac tgccttcttg ctgtgtcctc 38821 atattatctt tcccctgtgc aggcacatcc ctggtatctc ttcaagttct tttttttttt 38881 ttttttgaca gagtctcgct ctgttgccca ggttggagtg cagtggcgtg atctcggctc 38941 actgcaagct ccacccaggt tcacgccatt ctcctgcctc agcctcccaa gtagatggga 39001 ctataggcac ccgccaccat gcctggctaa ttttttgtat ttttagtaga gatggggttt 39061 cacagtgtta gccaggatgg tctcaatctc ctgaccttgt tatctgccca cctcggcctc 39121 tgaaagtgct gggattacag gcgtgagcta ctgtgcccag cctcttttca agttcttata 39181 aggacactag tcatattgga ttaagtccac acccttatga ccttctttaa cattaattac 39241 tgtctcaaat ttaaagcctt taaatttgaa acttgctatt ctctggacct ctggaaagtc 39301 attggtccct agatagcaag tagaataatg ataataaatg gtccctcttc taaaatgttt 39361 aacttactta tcttcagtta ttggtgaaag aagggtaagt ttttaaataa tattttacat 39421 ttgagaaaaa ttggagtatg caaaatcata ttaaaagtca ctaagaggcc aggcgtgatg 39481 gcttacgcct gtaagcctaa cactttggga ggccgaggtg ggcagattgc ttgagctcag 39541 gagcgcgaga ccagcctggg caacacggtg aaaccccatc tctactaaaa tacaaaaaat 39601 tagccgggcg tggtggcagg cgcctacagt cccagctact cgggaggctg aggcaggaga 39661 atcgcttaaa catgggaggc ggaagttgca gtgagcYgag atcgcgccac tgcactccag 39721 ccttggttac agagtgagat tctgtctcca aaaaaaaaag tcagtaagag tattaaaaac 39781 ggccccagat agttcatagc tttgtcatgt aacagagttt tgaagatgga attataagca 39841 catcgagtag ttacctctgt cctggattaa gctacctagt tctggattga aaacacattt
39901 gctagttcta cctgaagcaa ttaaatcctg ggaaaacata agcccacaat gccagtttac
39961 cgacttacag tgagaatgag agctccgtta tcaaagaaac attgaaagag caaagacact
40021 aactcagtct atgtttggat tggtgtagat ttctctcatg ccatttgcta tattgtattg
40081 aaactatctg tgtctttttc ccctacttaa aataagtaac ttttccagaa acttcttggg
40141 ttcttaggta ttatggttgg gaatcagata caaagatgaa taagaattac taaaaaaaaa
40201 atagtgacaa ggctaactta cagtgatctc agcaaaatgg tggactggga agttccaagc
40261 tcctgttcac cagcagaaaa attaaaaaat cacccagtag ctatttgaac cgacgttgta
40321 gaagctctga aatcagtcaa acgaagatta tagcaaccaa gcaaaYatct aaccaaaaag
40381 agccatcttt aaaccagtag gaaagatttg tggtattttt acacaccctt gccctaccct
40441 ctccctggca tagtagctga tttggtcttg aagaggcaac agctattccg cagctttcct
40501 ctcaaaccgg agggaacaga gcagacatta tttgcaaatt aatgtgtatg tctattccag
40561 cctttctggg ggatacctga aggcctaatg caaggtgctt gtctctgtct tgtataatca
40621 gggaagcagg cagtaaaacc agtgggcact gctattaaaa gctacagagt gactataaac
40681 ctacagacgc ctgaagagat tctggctgga catgtacaat aggccctgta aggcccagag
40741 gagaaactgg gtgagattct taggaaatta cgactctcaa aagcagccac atgtatgaag
40801 gaaaatagaa agctgtgatc atgtcaggca agacacatga tcagaaaagc cctgacaaga
40861 tgctaagatt catcccaggc tgatctctag attcagaata agcctagcta agagatgaag
40921 gagtacccct gcacagagcc agtctccaaa gactcagacg gctataatat gctctttttt
40981 gggggggcgg gggggtggtt gttgtagtgt ttgtttattt cagcttttgg cacttaagac
41041 agtcttgatt atactcttat aatagctgaa cacaaggtta aggaacctca gtgaccaaac
41101 ttgaaaagga atagtcttct gagtctcaaa tggactacta cagccttaaa cagtaatttt
41161 tttaaaaaac acagcaaatc ttgggggagg ggggacaatc tgatttccag agttaaccgt
41221 attataataa tcaaatgcca gattttcaac aacagcatca acaacaacaa caaaaaataa
41281 cagagcatac aaagaaacag gaaaacatgg ctcatttaaa ggaactaaag ggattgacag
41341 aaactgtccc agaggcagca cagatattgg acttattagc caaagactat ttgtttaaaa
41401 tatgcacaaa cacctaaggg gagaacatgg acaaagaact aaaggcaatc aggcaaacag
41461 tccctgaaga aaaggacact atcaataaag acatagaaat tacagaaatg aaacaaattt
41521 tggaactgaa aatacaataa ctgaaatgaa aaatttacta gagggtttca acagcagatt
41581 gatgcagata gaagaaagaa ctagcaaagt agaagttagg acRattaatt atcaagtttg
41641 aggagcaaaa agaaaaagga aggaagaaaa gtgaacagag tctaagcgac tcatgggact
41701 aataagtgga ccatcatatg cattgtggga gttttagaag ggggagaaat actgggcaga
41761 aagattattt tgagaaataa tgaagaataa actccccaaa tttgatgaag ggcatagacc
41821 cacaaatcca aaaatctcac tagactctaa gtagaatcaa ccaaagagac tcacactgag
41881 acacatgatc aaacttgaca aagagaaatt cttaaaagca cccagagaga ggtgactcag
41941 cagatacaat taatcttcaa gaagactata agctgaattt ttaccagaaa ccttggaggc
42001 cagaaggaag tggggtgata tatttaaagt gctgcaagaa aacaattgta aaacacaaat
42061 tctatatcca gcaaaaccat ccttcaacta tgaagcagaa gttaagacat tgccagataa
42121 gtgaaagttg agggagttaa ttaccaccag acctctatag gaaatatgaa agggagaccc
42181 gcaggttgaa atgaaagaat acttagacaa taattcgaag gtgtatgaag atattaagat
42241 ctccgataaa gctaatttca cgagttaata tttataattt tggtttataa ttgtgatttt
42301 tgaagaaatt ataacacaaa ttcaggtcag tYatgtcctg attatatccc agtaggttag
42361 actctggtaa gacaatttct cttttgggca ggccttgtta aggacagaat gcttcagagg
42421 tatttcaaaa tggctaacat ttcccctccc tatgccagga gtatgggggg atttttctcc
42481 cRtcttcact gagagaacca gggaggaccc ctggagataa aactcacgag aatgtctggg
42541 ggggcctgct aagactgggc cccatagagt ttttaactct cagacttgtt ggcactgagc
42601 ctccaatgat tcatcattta tagtttaggt tttcctactc tggtactggt ggttcctgca
42661 gaggcttctt ctcctgagtt tctgctctgc aggttgggat tctttatctg cctgtctgtY
42721 tctccaatct ggagacagtg ttttgacctg tgacctcact tctctgacaa atctaagaag
42781 agttgatttt caggttgttc agctttttac ttgttgtcaa gatggatgag ggtttccaag
42841 ctgtttgcat gctagactga gaaccagaag ttctgcatgt tggttttgta tcctgcagcc
42901 ttggtgaact catttattta ttctaatatt tttggtgtgt ggaatatgta gggttttcta
42961 catctaagat catgccagct acaaacagag ataattttcc tacttccttt ccaacttcga
43021 ttcctttttt gtccttttct tgacaaactg ctcttgctag aaattccaat atcatgttKa
43081 atagaagtgg tgaaagtggg catcgttgtc ttgttcctga ttgtagggga aaaacttttg
43141 agtctttcac cactgaatat gctgtcagct ataggttttt catatatgac ctttatcatc
43201 tatagaaagt tctttctatt cctagtttac tgagtgtttt tatcttgaaa ggattgttgc
43261 attagttcag atgctttttc tgcattagtt gggatgatca tgtcatccca actttattaa
43321 tatgttgtaa tactgtaatt gatttttatg tgtttcagga agaaatccca cttgttaggt
43381 acttaactcc agccgcctct agtcatcttt ccacccaagg ggagtgggga ctgagacaca
43441 gtggtgaagg tcacagccca agggtatagt cttactgaaa gactgagacc taatcatagg
43501 tctatagaat ccttcccccc tacacacaca ccttaccacc acaacactaa agccttgttt
43561 actacagttt attttcctgg gtaatcatgt ctggctttca gcaaaaagta caaggcatac
43621 taaaaagcaa aaacagtttg aggaaatgga gcaagcatca gaactctgat atggaagaga
43681 tgttggaatt atcagactgg gcatttaaaa caactctgat taatatgcta agggttctaa
43741 tgggaaaagt agataacata agtagataac atgcaagaac agatggacag tgcaagcaga 43801 gagatggaaa tttttagaaa gaatcaaaac gaaacactag agataaaaaa aactagcaga
43861 aacgaaaaat gtttttgatg gactcaacct ctgagcttga ggatatgcca acagaaacat
43921 ccaaaactga aggcacagag agaaagatga cctaaaaaat ggaacagaat atacaagaag
43981 tgggcaacta cagaagatta acatacttgt aaatgataat accagaaaca gaagatgaga
44041 acaaaaatat ttgaagcaat tatgactgag aatttcccca aattaatgcc agacactaaa
44101 ccacagatcc atgaagctca gaaaacacca aacaggataa atgccaaaaa aaaaaaaaaa
44161 aaaaaaaaac atctaggcat atcataatca cactgcagaa aatcaaagat aaataaaaca
44221 taagaagaca gaggggaaaa gtaccttagc tataaaggag gaagaataag aattacatcg
44281 tacagtattt ggattgtata gtaattctgt gtttaacttt tcaaggaact gccaagcttt
44341 tcatggtggc tgcaccattt tacattccca tcagcagtgt acagggtttg ggattctcca
44401 caccctcacc aacKcttatt atttcccccc tttaaaagaa atcataacca tcctaatgga
44461 tgtgaagtgg tatctcattg tggtttagat ttaatttcct taaaggctag tgatgttgag
44521 caacttttca tggtatatct tctttggagg catgtctatt tgaactcttt gcccatKtta
44581 aaatttgttc aattgtgttt ttttggtcgg tgagttgtag gagttcttta tatattgtgg
44641 atgttaatcc cttatcatat atatatgctt tgtacatatt ttctcccatt ctgtatgttg
44701 tcttttcact ttcttggtag tgtcctttga tgtttaaatt ttttttgatg aagtccagtt
44761 tacctatttt gttcttttgt tgtctgtgct gttggtgttg tatcaaatca ttgcctaacc
44821 cagtgtcatg aagatgtttc tctatgcgtt cttccaaaat tttatagttt tagctcttaa
44881 atttaatagt tttagctctt aaatttaggt gttttatatg ttttaagcta atttctgtat
44941 ttgacttaat ataagggtct aacttcatca ttgtgcatgt ggatatccag ttttcccagc
45001 actgttgttg gaaaggttgt cctttctcca ttaaatgatg ttggcatcct tgtggaaaat
45061 cacttgacca tatatgcaag gatttatttc •tgggctttct gttctatacc tttgatctgt
45121 atgtctgtct ttatgctgtg gggggacccc acactgtttt gatcatctag ctttgtaata
45181 agttttgcaa tcagacgttg aaatgtaata aagatgaata aatacggtat tttttaatcc
45241 taaaatgtta gctccatgag agcacagatt tttatctatc cttttttttt tttctttttt
45301 ttgagatgga gtctcactct gtcgcccaag ctggagtgca gtggcctgat ctcagctcac
45361 tgtaaccccc acctcctggg ttcaagtgat tctcctgtgt cagcctactg agtagctggg
45421 attacaggtg tgatgcccgg ctaatagatt tttatctatc ttattcatgt attagcccta
45481 atatctagac aggtatctag tatataataa gagataaata aaagctgttg aatggccagg
45541 cgcggtggcc cacccctgta attctagcac tttggggggc cgaggcgggt agaacacctg
45601 agatcaggag ttcgagacca gtctggccaa cgtggtgaaa ccccatctct actaaaaatg
45661 caaaaaatta gccaggtgtg gtggcaggca cctgtaatcc cagctacttg ggaggctgag
45721 acaggagaat tgcttgaacc tgggaggcgg aggttacatt gagctgagat cacaccattg
45781 cactccagcc tgggtgacaa gagtgaaact gtctcaaaaa acaaaacaaa aactgctgaa
45841 tgaatggaat ctcccagaga gcttaagagt atgatggaca ggccaaaata ggaaattgaa
45901 taggaataac agcatttgac aaattagcag ttttcctttt tccgccttcc aaaaaaccct
45961 ttcctcactc ttaaataatg taacttcagt atgagtattc ctgtagcact ggattcagtg
46021 tgtgcacaat taaacttcag cagccaccgc tgcaagtggg ctcccagaac ccatctccta
46081 agtacttctg tccgaatctt atccccaggc attgggcagg attctggatg agttacttac
46141 gatgtcttca cgtgttagat ttgtgccttc caatcctcac actaccattc ttttatacag
46201 ttactactac attttaaagt ggcaccttgg tctgttgggg atgggcaaga aaggagctct
46261 agagtattag taaatttaag gattatttgg aaatagaaga attgtgagga aggacjictca
46321 gcttgggacc ttatctgatg tagtgcccag ccattgcaga tagtcattta agctagaact
46381 ttacatgact tgtagtggcc ctctgctctg gagtcttcct gggatatact gccttctagg
46441 attggcataa aaagctacct gtatttaaat ctcatgtgta gttcatgaaa aataaaatac
46501 aaagctgctt gtgaaactta cctaggatag ttgaacaaga gagtggtcag gccaaggctt
46561 cattaagaag tataaaaagg ccgggcgcag tggctcacgc ctgtaatccc agcactttgg
46621 gaggccgagg cggacggatc acaaggtcag gagatcgaga ccatcctggc taacacggtg
46681 aaaccccatc tctactaaaa atacaaaaaa ttagccaggc gtggtggtgg gcgcctgtag
46741 tcccagctac ttgggaggct gaggcaggag aatggtgtga acccaagagg cggagcttgc
46801 agtgagccga ggttgcgcca ctgcactcca gcctggctga cagagcgaga ctctgtctca
46861 aaaaaaaaaa aaaagaagaa gaagtataaa gcattcattt ctttgccata atgcagaggg
46921 ataaaagaag tgacgattgc cattatttag cttctttgaR gttgtaagaa acaaattggt
46981 ttagtttacc ttccaagttt attataaaga tacatatctc agtKggcctt cacgaaaatt
47041 Maacaccatt taccttttat gtcctctagt tcacctgttg ttaccaactc tagtatttcc
47101 accattttga ccaatcagcc tgtatctcct tataatgtac ttctatttct ctagactata
47161 agctctccca ttttcttatt tctcttggct tcatacttct taagagaaaa gatgtgattg
47221 ggttgtccag tcatcatcca ggtgagcagt gctctcatga ctcacctctc aggtccttga
47281 tggtctttgg gccagagctg atctctggtc atagtccctt agagccagtg gtggtgcctc
47341 gtcgaagtca tggtgctcat actgagagaa atgtttatgg ccaatagtcc cttagagcca
47401 gtggtggtgt ctcgtctaag tcgtggtgct catactgaga gaaatgttta tggccagtag
47461 tcccttagag ccagtggtgg cgtctcgtca aagtcgtggt gctcatactg agagaaatgt
47521 ttatggccag tagtccctta gagccagtgg tggcgtctcg tcaaagtcgt ggtgctcata
47581 ctgagagaaa tgtttatggc cagtagtccc ttagagccag tggtggcgtc tcgtcaaagt
47641 cgtggtgctc atactgagag aaatgtttat ggccaatagt cccttagagc cagtggtggc
47701 gtctcgtcga agtcgtggtg ctcatactga gagaaatgtt tatggccaat agtcccttag 47761 agctagtggt ggcgtctcgt caaagtcgtg gtgctcatac tgagagaaat gtttatggcc
47821 aatagtccct tagagccagt ggtggtgtct cgtctaagtc atggtgctcc tactgagaga
47881 aatgtttatg gccagtagtc ccttagagcc agtggtggcg tctcgtcgaa gtcgtggtgc
47941 tcatactgag agaaatgttt atggccaata gtcccttaga gccagtggtg gcgtctcgtc
48001 gaagtcgtgg tgctcatact ggaagaaatg tttatggcca atagtccctt agagccagtg
48061 gtggcgtctg gtcgaagtcg tggtgctcat gctgagagaa atgtttatgg ccactagtcc
48121 cttagagcca gtggtggtgt ctcgtcgaag tcgtggtgct catactggaa gaaatgttta
48181 tggccaatag tcccttagag ccagtggtgg tgtctcgtcg aagtcgtggt gctcatgctg
48241 agagaaatgt ttgtggccac tttccttaga aggagcccgt gtggccttgg tagagactcg
48301 gagcctcaca tatcattctt tactagtgca tcactgggcg ctttcatttt gaaggagaag
48361 tagaaggggc tgcacatgca tggtatgtat ttctggctcc cagagggagt agccatggtt
48421 atccaaacaa aataacatta tcttcacttg tcctttgttc tataactttt tgtggaagtg
48481 ttttaggaac caatagcaat tcaggtatat tacacttctg ttttccatcY ctttttattg
48541 aattattgtt tagggctttt gctagaatta gtgaaatctg actatttaga aagaaaagat
48601 ccatgtagct gggccattaa agaagataaa ggaaaagaat gggctgtgtg aggctgccca
48661 atatgcaagg agttaaagca gcttggagca gagttgctct gtgattttct ttagcggtta
48721 tcataagcca ggacccagag accttcatca aggtgttctg Yatgaaggcc ttctttgggg
48781 atggcatgtt ttattagagg catggcagct aaagaataat attttgtagg tagtccatgt
48841 acctacttga tcttctctcc aagattagtt gagaaatttt gagaccatgg taaacaggtt
48901 tctgatgttt gcccaaaccc ataaggaatc agatgctagt tttgaaaggg attgccatgc
48961 aaKtagttat cttgctttca tcaatgatat aaactcagct tcgtgaaaat taccgtgtta
49021 tattattatt tttctcattt caacaaagac cggtgaaaac ttcttcgtgg actggaacca
49081 ccgggttgct catacagcga ggtgRactcc tagctggttg ataaccatac ccagagagtg
49141 ttgatgccgt cccRaaggga agagttccag cccaagagtc tggcccatat cacgtgccac
49201 tgagggtgcc tgtgcaggat tggaagcctg gggaagaggg gaagatggag ggagaaaggg
49261 cagaaggccc gggcaggtct gggaggagag gtgtcgaagg gtcaatagga aatgcttaga
49321 caaaggcttt aaagctctga gggctttgtt ctgggactaa aaaggagaag gaattcttag
49381 aactcttctc tttcccttgg ctccttccag tgaaggtcag atttgagaga gaaagcagtt
49441 aacttggctt acctaagaga ctttattgtg gaatattttt ccatcctctg tggttagtaa
49501 ccatagccta cgcagacggg aaaacctgac ctcagtgcaa ggtttctctg ccgtttgaga
49561 tacattgtct ctgattttac ttttctttct ccttcaggct gcctgatagg ggctgtaaat
49621 ctcaaatcca gcaatcgaac cccagtggta caggaatttg aaagtaagta caaacgaaat
49681 gcctaggata atgggcacca tctactggac aYttgatgtc ttgcagccaa ctcaggacac
49741 atctgtctct gtaagtgtgc cagggagatg ttccgggtgg actgctctgc ggtgcacagg
49801 cctggaagca tggcatcagc aggccggcag tgagccctgt gcagttttcc atcagctgtc
49861 atcgcactcc tgcctggctt tgtgatcaca caacaaataa atttcttttt tctttttgag
49921 atggagtctc gctcctgttg cccaggctgg agtgtagtgg cgcaatcttg gctcactgca
49981 gcctctgcct cctgagtagc tgggattaca ggcacccgcc accacaccca gctgattttt
50041 gtacggggtt ttgccatgtt tggcctgact ggtctcaaac tcctgacctc aggtgatcca
50101 ccctccttgg cctcccaaag tgctaagatt acaggtgtga gccaccgcgc ctggccaaga
50161 aatttcttat atacaagcag ggtgtttaag ggaggggtac actatgtagt aaaatccttg
50221 ctaattatct ttattgtttt tctggcaaaa aatcctttct caaagtctct tggagtgtgg
50281 agttggctac gtttttaatc tRcatgaaag gcctcttgaa ttagagcttg cagggtctgg
50341 gtgcagctga acgtagaagc actccacatt caccgagagc tctttttctt ctttgcgtgt
50401 taggtgtgga actgtcttgc atcattacgg attcgcagac aagtgacccc aggatcgagt
50461 ggaagaaaat tcaagatgaa caaaccacat atgtgttttt tgacaacaaa attcagggta
50521 tgatcctgta gtcctcttgc ctgctgacct ttcctctgtc catagacctg ggtRtacact
50581 cttggccaga aacttacctc agactggtaa cctgcatctg tagctaggRc cgtttttttt
50641 tttttaaaga tttataatta tggaaaattt caaatatatg caaaagtaat gcaaatggta
50701 tatcaagcct ccctgtccct gtcccccagc atcaacaagt gcagttcatt agctaggatc
50761 ttaatctgct cacagcccca tttctttggg gaatcaaagt gaacattttc ctaggccatc
50821 ttctcatgat cctgatgtga gtgatgtagg tgagtgaatg agaaggacca tatgtttcag
50881 caagggcaca gcacaaatgg actgagccac ccactgtgtt tctggcttgg tgctggggtt
50941 gccgatgggg agaaaacacg gtgtaccctg aagtgacatc ctcccgggtg ggaggatggt
51001 ggtcagggaa ggctccccaa agtggcctct ctgggtgcgg tgtgaagggt ggctggttag
51061 caggcaagat ggtatggagg gaggtggtga tcaggagaga atgctgtgta tgaagacgca
51121 gaagcaacag acggactggc tgatgggaaa gactggaagt tccccactgt tgccggtaca
51181 tggacaggga gagagggagg agagggggga gaggggctgg agaggttgcc tggccaggca
51241 gactgcactg aatgccaggc gagcatgggc ggtatctgag ggcccgctgt gaggctctca
51301 ggggaggtgc cgtggctgca tctatgtttc agaagactga agtggctgct gtagagaaga
51361 gattggaggc ggcacaactc agagcagagg cccaagtcag aggccttctg cagagtccgg
51421 gagagggctg gtggggggcc ccaagctggg gtgcaggcag ggagagagac tcgaggggag
51481 gcctgctggg gcttggctgt gccaaggggc acagagggag gcaggcaggt ggctggagag
51541 gaggatggca tcctttttct gacaggaggg cgggagctgc aggaggtaca catgcaggct
51601 cgtgcatgag tcggagcctg tggagttctc agagaagttg agagttgaga gggatttgtg
51661 gaagcagctc caacacagtc ttgctttggg ttctcagtgt cgccgggagc ctgagtctcc 51721 tcaaaagagt tttgcttccc actcacgtga agtccctcct ttgcYtctta tttttggagt
51781 gatggaaatg ttcttctcag aacacagatc tgttttcttt ggctactcag gctgaccctg
51841 ctaaaccctg agcatgtagt tagagcatca gaggaaccag gtgagaaact ctttaaagaa
51901 ctttcctttt ctggggatta aacattttgc tcttagccac agggatgggg aggccgaggg
51961 agacggtttc ccgcacagaa tgccggggtt cggggaggat gcagctgtac ttggcctctt
52021 tcctcgcctc agtgcagggc tgcaccagag gcaccctggt ggggtttatt tactcttttc
52081 tcacatggta aaggttcgaa tcctccacta atgggtgggt gtataacaaa gcttcccatt
52141 cgaaaatgtt gttgccaacg tctgtgctcc atcggaagca tcttttagtg tgctgttttt
52201 agtgtattat ggcaagccct tcatgtcctt gtcagcagtt gtcaggttta gcgatgagta
52261 gttgctactc ttttgtgtca tgttttccta actccttccc ctatttgttg ttttccaggt
52321 tcttcctgct tctttgaaag tgttattatg tcttatgttt aaaatgtttt tttatggagc
52381 tgtaattcgt accgcatcct tttaaagtgt gcagttcagg ggtttttaat atactcacag
52441 ttgtgcaacc atcaccacta tcaaattcca gaacattttc atcatcccac agagaaagcc
52501 cgtacccagc agtagtcagt cctcctcccg catccctggc agctctgatt ccactttctg
52561 tctctgtgga tttgcctgtt ctggacactg gatagaaact gtggtcttcc atggctgacg
52621 tctttcactc agcatcctgt tttcaaggtt catctgtgtt gtagcctgtg tccgtacttc
52681 atttcttttc acggctgaat aatgttccat tgtagatata ccacattatt ttacccattc
52741 tattgatgga catttgggtt gtttccactt tttggctatt atcagtaatg ctgctatcag
52801 tatttgttta caggtttttg tgtgaaccta agttttcatt tctctgggat aaatgaccaa
52861 gagtacaact gctgagtctt acagtagttg catgtttagt tgtttaaaaa accgacagag
52921 tgtttttcaa agctgcttta cgattttaca ctcctaccWg cagtgtattt tcccacatcc
52981 ttaccaacac ttgttattgt ctgacttgtt aagttctagc catcttagtg ggttggaagc
53041 ggcgtcttgt ggatttgatt tgcatttcct ggtgactaat gatgttgagg gtcttatttg
53101 tgtattttct ttggagaaat gtctgttcaa attttatgtc catttaaaaa tatttttttt
53161 tctagaaaca ggatctcact gtgttgcaca ggctggacat gaattcccag gctcaaataa
53221 tcctcccacc tcagccttct gagtagctga gactatatgt gtgcaccact gcacccaccc
53281 ttatgtccag tttttaaatt aggttgtcta tttcttactg aagtgtgaat tctttattct
53341 ggatgctagg ttggatgtac gatttgcagg tatttctccc attgtgtgga ttgttttcac
53401 ctgcctttgt cctttgaatt ttgatgaagt ccggtgtaac tgtttttagt gtgtgcttgt
53461 gcttttggtg tcatatctaa gaaaccattg cccaatccaa ggtcatgagg gatttatgct
53521 tatgcatttt tctaagagtt ttatagtttt aactcctatg ttaggtcttt gttgtatatg
53581 gagtgagaga gaggttcaac ttcatgtgtt tgcaagtgga tatcaagtta tctcagcatc
53641 atttatgaaa cattggttgt ttttctagtg gtgttttttt ctattcatgg gcggtccttc
53701 ctggaaatga ggaggaggag gtgaagttcc tggttttaat gacggagaca catggtaatg
53761 agtgtgtctt gcacaccagg aactattttt ggtgtttagg ttacagatag gaataggtca
53821 tggttcctgt cctcaagaga gttcacttct gtactcggga atagaaataa atattgatct
53881 gcaggcttcc ttgctgtggc atctagtgct agccccagaa accaccctct tcaagtggca
53941 aagatttctt taaagaagtt ttttagtgcc tcgtgtcttt tctgtaggag acttggcggg
54001 tcgtgcagaa atactgggga agacatccct gaagatctgg aatgtgacac ggagagactc
54061 agccctttat cgctgtgagg tcgttgctcg aaatgaccgc aaggaaattg atgagattgt
54121 gatcgagtta actgtgcaag gtaggagctc atgcgaaggt gagacattgc caccatagga
54181 tgcaagagat cagttagtgt cttggtttct ttccttctag aactgtatcc ctgagggctt
54241 cacatggctt agggagggga gaactgttga gagctgagac tgcctgagtt ggggtagaag
54301 gtgagaaggg aagtgaagtt cctgtcactt ccttccacca gctagaggag gcgctttaac
54361 atatgggagg gcaggatgca gcggtggctt gtcagtctgc agttgtgatc ctgggaacag
54421 cagtggcgtg ggaacccctc gactggctgt cttgtctttg gagctgattt cttctgggct
54481 ttaataagaa tagagccctg tcactgagat cttaaacacc accccttttt ccccacagtg
54541 aagccagtga cccctgtctg tagagtgccg aaggctgtac cagtaggcaa gatggcaaca
54601 ctgcactgcc aggagagtga gggccacccc cggcctcact acagctggta tcgcaatgat
54661 gtaccactgc ccacggattc cagagccaat cccagatttc gcaattcttc tttccactta
54721 aactctgaaa caggcacttt ggtaagatct cttctaagag gtgaggatgg agatgtcttt
54781 gttggggcaa gagatgctta ttgtgaaaag aagattagga gagatactga aacttcttga
54841 taagacagga gtcaaaaatc tagaatgtta gggattctac aggaaaaatg acccttcttc
54901 cttttataaa caagtacatt cttagggggt aaaataagga agagaaagaa accatagatt
54961 gagaccgaag agggatatta agcaaatgtt actagggccc tgatcggata atcaactgta
55021 aaagtcatta gacaatcggg gaaattggat tactaggtgg tatttgatga tctattaagc
55081 aactgttcat tgctttaggc atgatgaagg tattgtggtt acgatttttt acagtcttgt
55141 agagatactg aagtatttat ggataaaagg tgtttggaat ttgtttcaaa ataacccagt
55201 ggtggtggtg ggaggtgggg gaggagagga aacagattgg tcatgcacta gaggacaaag
55261 tgacctggcc agcaaaccac accaggcttt tactgcatgt ggYtctctcc tgggaggtac
55321 cttctgatct gtggaccatc cagttccacg tagtcatcag acagatttta acagggtcct
55381 ctgtgttcat tttgccaaaa tgttgcccca ggagcagtca tcattgaaga gcccttttac
55441 cagaataaaa gcaaggttcc acctcccatt tcggatgttg ccaggctttg gtgtctccag
55501 tcccaagggg attggttgtt ggggagctgt caggcaggta tcagggagtg ggtcagggag
55561 gaacatgcac agtgctgggg aagctgaaag caagcgagca ggtgtcgacc tagttctgga
55621 cccagctggc tgtgctctca gaaatcggcc ccatgatggg tccgattatt tacttgtcat 55681 tccctgaaac aggtgttcac tgctgttcac aaggacgact ctgggcagta ctactgcatt 55741 gcttccaatg acgcaggctc agccaggtgt gaggagcagg agatggaagt ctgtgagttt 55801 cttttttgaa gaggtttcat cgcaagactg ggaagtgaat agaacatttt aatttaatat 55861 tataccatca gcttagagaa ctcttccgcc atgggttagc tttcttctgg agctgcctag 55921 gtccaccaga acccactgca cagtgaaggg aaggctgaga aaaccgagtc cagataagca 55981 ggtcctcaaa acaaaacctt ctgggatctg ctggtgttgg gaacgtggcc agccttgcaa 56041 ctttcactgc aagtttagta aactccattc tttggtaatt gatagaatta tcaaaatgta 56101 aggtggtgtc aggtctccag aaatttatcc agttataacc ctcttgtaga aaactgacag 56161 ttttagcccg agtaatttta cctgcagaca aagcttagcc cctagagtaa tgttatctca 56221 gggctctggt tcccctaatg gaggggcgtt gagaggaggg tgggggcaga gaggatgtgt 56281 ccaaatcact tggggagctt ttttttttct taaaaaacaa aaaacaaaaa acaccacagg 56341 gtcttgcttt gttgcccagg ctggagtgca gtgagtgcag tcacaggtca ctgcatcctc 56401 aaacccagcc ttcaagccat tctcccacct cagtctcctg agtagctgga acaacaggca 56461 cacaccacca tgctcagcta attttaYttt ttgtagatac ggggtctcac tgtgttgccc 56521 aggctggtct caaactcctg gcctcaagca atcctcctgc ctcggcctcc cccaggtgct 56581 gggattaaca ggcatgagcc actgtgccca gccaggagca ttttttgaat ccttccctcc 56641 tccctatttg gagctctcct cagagcagca ttttccaaaa ctgcctggtg atacccattt 56701 gggaccactg tttcacatac agattcgagc ccacctcaga catatgctag aaatgtStgt 56761 gtgtgagaca agcgttccga aggattcttc tcatgtgtca cgtttgagaa actctgctct 56821 agggttgttt tctcctgcag ttttgcccca gacctacttt agcctattga ctttttagtg 56881 atgaaacttg ggaattgaca ttttaacatc aaaattgcat gccaggcgtg gtggctcaca 56941 ctataatcct agcactctgg gaggctgagg cgggtggatc acctgaggtc aggagttcaa 57001 gaccagcctg gccaacatgc caaaactctg tgtctactaa aaatacaaaa attagcctgg 57061 tgtggtggca ggcgcctgta gtcccagtaa cttgggaggc tgaggcagaa ggatcccttg 57121 aacccgggag gcggagcttg cagtgagccg agattgcacc acagaactcc agcctgggtg 57181 acggtgacgg agcaagactc tgtctcaaaa aaaaaaaaaa aaaaacgatt gcaagtttac 57241 tttttgtttt tgagatggag tctcgctctg tcacccaggc tggagtgcag tggcacaatc 57301 tcggctcact gcaagctccg cctcccgggt tcacaccttt ctcctgcctc agcctcctga 57361 gtagctggga ctacaggcac ctgccaacac gotcagccaa ttttttgtRt ttctagtaga 57421 gacggggttt caccacctta gccaggatgg tctcgaatct ctgacctcgt gatctgccca 57481 cctcggcctc ccaaagtgct gggattacag gcRtgagcca ccgcgcccgg cctgcaagtt 57541 gatttttaag tgtacattca tctttgagaa ccaatacact aggataaagg agattttttt 57601 aaaacttcca agttacttgg ctcttgttga taaattccct gcatagtttt ccatgtgtcg 57661 cttgcttgaa ggaacggctg ccagagcccc atatgaaagt gacccagtgc ctttgcccag 57721 tgtatagaca tgagaaaagg gttccagggg gtttoctctc agagggctta tagatcggag 57781 gtcaggcagc cggcagagac aaagctctag ttcttaccta ggattcttta cttgtttgtt 57841 ttttgagaca gattctttaa ggcttgaagc tacctttctt ccccgcagca atctgcaaca 57901 aagggaggcc ctggaaatta gggattcttt tcttcttggg atcctggctc ctaatcttgt 57961 cctcataacc atttcctcca taagtaatct caggcattca gccagaggat ttttaagcct 58021 acagtgccaa gcccacttta atacactttt cagtgcgact gcatttcaac tgtagaagtc 58081 tcagtacttt tcacgtgtga agttaggagt tatggtgtcc tatatgttct aggctagaag 58141 gattgtaagt gttctctctt ctagctgggg taggcctgag ggggcagggg gcaagtgggt 58201 ttggggtgaa ggaaggaggc acagcagggc cagggccttt ttaaataaca gatagtgtgt 58261 atcatggctt ccaccaaacc tccttttctt cagatgacct gaacattggc ggaattattg 58321 ggggggttct ggttgtcctt gctgtactgg ccctgatcac gttgggcatc tgctgtgcat 58381 acagacgtgg ctacttcatc aacaataaac aggatggaga aaggtgagcc tgccttatgt 58441 gaaaaaaggg aagttcaagc tggcaataat ataacaaccc tgttgctaaa ctgctctctt 58501 ctcctcaYag ttacaagaac ccagggaaac cagatggagt taactacatc cgcactgacg 58561 aggaggtaat catttagtaa acctggaaac ctaggtRtac ccagcaggga aaacaacatt 58621 cccccttgga aaccacacgg gtctcctggg aagatttctg agtgattgtg cagctcctga 58681 gctcctcagc cccttcacag tMacacacac acaca'cacac acacacacac acacacacac 58741 actaatggga tttgtgcttt gcaagcgcta gtgatggtac tttttaagaa tgattattcc 58801 atctgtattt agcccatgtt agacttactc cgtgtttttc cctgcttgcc accaggcccc 58861 ttgatggctc taactgtgtg ttggctttgc agggcgactt cagacacaag tcatcgtttg 58921 tgatctgaga cccgcggtgt ggctgagagc gcacagagcg cacgtgcaca tacctctgct 58981 agaaactcct gtcaaggcag cgagagctga tgcactcgga cagagctaga cactcattca 59041 gaagcttttc gttttggcca aagttgacca ctactcttct tactctaaca agccacatga 59101 atagaagaat tttcctcaag atggacccgg taaatataac cacaaggaag cgaaactggg 59161 tgcgttcact gagttgggtt cctaatctgt ttctggcctg attcccgcat gaRtattagg 59221 gtgatcttaa agagtttgct cacgtaaacg cccgtgctgg gccctgtgaa gccagcatgt 59281 tcaccactgg tcgttcagca gccacgacag caccatgtga gatggcgagg tggctggaca 59341 gcaccagcag cgcatcccgg cgggaaccca gaaaaggctt cttacacagc agccttactt 59401 catcggccca cagacaccac cgcagtttct tcttaaaggc tctgctgatc ggtgttgcag 59461 tgtccattgt ggagaagctt tttggatcag cattttgtaa aaacaaccaa aatcaggaag 59521 gtaaattggt tgctggaaga gggatcttgc ctgaggaacc ctgcttgtcc aacagggtgt 59581 caggatttaa ggaaaacctt cgtcttaggc taagtctgaa atggtactga aatatgcttt 59641 tctatgggtc ttgtttattt tataaaattt tacatctaaa tttttgctaa ggatgtattt
59701 tgattattga aaagaaaatt tctatttaaa ctgtaaatat attgtcatac aaYgttaaat
59761 aacctatttt tttaaaaaag ttcaacttaa ggtagaagtt ccaagctact agtgttaaat
59821 tggaaaatat caataattaa gagtatttta cccaaggaat cctctcatgg aagtttactg
59881 tgatgttcct tttctcacac aagttttagc ctttttcaca agggaactca tactgtctac
59941 acatcagacc atagttgctt aggaaacctt taaaaattcc agttaagcaa tgttgaaatc
60001 agtttgcatc tcttcaaaag aaacctctca ggttagcttt gaactgcctc ttcctgagat
60061 gactaggaca gtctgtaccc agaggccacc cagaagccct cagatgtaca tacacagatg
60121 ccagtcagct cctggggttg cgccaggcgc ccccgctcta gctcactgtt gcctcgctgt
60181 ctgccaggag gccctgccat ccttgggccc tggcagtggc tgtgtcccag tgagctttac
60241 tcacgtggcc cttgcttcat ccagcacagc tctcaggtgg gcactgcagg gacactggtg
60301 tcttccatgt agcgtcccag ctttgggctc ctgtaacaga cctctttttg gttatggatg
60361 gctcacaaaa tagggccNNN aatgctattt ttttttttta agtttgttta attatttgtt
60421 aagattgtct aaggccaaag gcaattgcga aatcaagtct gtcaagtaca ataacatttt
60481 taaaagaaaa tggatcccac tgttcctctt tgccacagag aaagcaccca gacgccacag
60541 gctctgtcgc atttcaaaac aaaccatgat ggagtggcgg ccagtccagc cttttaaaga
60601 acgtcaggtg gagcagccag gtgaaaggcc tggcggggag gaaagtgaaa cgcctgaatc
60661 aaaagcagtt ttctaatttt gactttaaat ttttcatccR ccRgagacac tgctcccatt
60721 tgtgggggga cattagcaac atcactcaga agcctgtgtt cttcaagagc aggtgttctc
60781 agcctcacat gccctgccgt gctggactca ggactgaagt gctgtaaagc aaggagctgc
60841 tgagaaggag cactccactg tgtgcctgga gaatggctct cactactcac cttgtctttc
60901 agcttccagt gtcttgggtt ttttatactt tgacagcttt tttttaattg catacatgag
60961 actgtgttga ctttttttag ttatgtgaaa cactttgccg caggccgcct ggcagaggca
61021 ggaaatgctc cagcagtggc tcagtgctcc ctggtgtctg ctgcatggca tcctggatgc
61081 ttagcatgca agttccctcc atcattgcca ccttggtaga gagggatggc tccccaccct
61141 cagcgttggg gattcacgct ccagcctcct tcttggttgt catagtgata gggtagcctt
61201 attgccccct cttcttatac cctaaaacct tctacactag tgccatggga accaggtctg
61261 aaaaagtaga gagaagtgaa agtagagtct gggaagtagc tgcctataac tgagactaga
61321 cggaaaagKa atactcgtgt attttaagat atgaatgtga ctcaagactc gaggccgata
61381 cgaggctgtg attctgcctt tggatggatg ttgctgtaca cagatgctac agacttgtac
61441 taacacaccg taatttggca tttgtttaac ctcatttata aaagcttcaa aaaaacccaa
61501 acattgcttc attctttgtt atttgctctt acgttgggtt tgtctcttct tcctagcatt
61561 tcagtggtta ggcatgcaca ctaaaaatgc tatcaatcac ccatccacca gggaagtcag
61621 tgctgggcag gaggtcagcc tgtgtgctca agagcagtgc tgcgcctctc cgccaccgag
61681 cccacctgtg ttgcggttcc cacttgggat Rgcagtgggc agatctcagt ggcatcccat
61741 cccccaaacc aaccaacgca catcatcact ttgtactttt gcttttgtaa tactgggatt
61801 aaaaaacaaa ccaactgcat ttctttctgg atattgttga acaaaaatag cattcagttt
61861 acccactagt gctaacagaa gagactcaag ctgttccccc atcatgggag cagcccttaa
61921 cagagggctg cacaaatcKg cagtgctgct ctggggaagg ctgcaaagcc attttttccc
61981 aagaagggat gctgttcatg tctgttaggg aaagcacacc gcctctgcct gggcacagat
62041 gaactgccct tcaagacaat catctttttt ctaataggga agatttggtt tcattcctct
62101 aacaaacaaa catgctttat gtaaaattaa aatgattacc aataagagct ctgttctaaa
62161 aacagaaaaa tcctattgac tactggaagg ctgtatttat gctaaacctt tattactttt
62221 agaaagctgt acactttttt aaaaaatttg cacttataaa taatagaaaa aatattagaa
62281 tcaaaatttc gtcattacag atgggaaaat atgtactata aggaattcaa gttaacagag
62341 gcttgattta tataaaagaa agctgcagtt ttaaagttgt gttccttaaa gaccagatta
62401 tagcttatct gaatgggctt gacactttca aatggaataa agttacaata ttaaacagat
62461 tagggtaaga aaatctaaat ctggcacatc tctattattt gacagtgttt aaccaaatac
62521 atcataNaga atagaaggtg agtgccaggc ccctgaggag gagctctcgt gttccacagc
62581 aaagcgctgc ccaaggactg cgggaagtga tccagctgcc ttcacacgga ggacacgaga
62641 ctgcttcctc aagggctcct gcctgcctgg acactggtgg gaggcgctgt ttagttggct
62701 gttttcagag gggtctttcg gagggacctc ctgctgcagg ctggagtgtc tttattcctg
62761 gcgggagacc gcacattcca ctgctgaggc tgtgggggcc tagggaaagg acatgtgcag
62821 tcattctgga actcagaaaa accctgacag aaacatccac ctcaaaagga acactgctaa
62881 acttacggtt tatcaggcag tgataaacat aagatgtcat ttccttgact ccggccttca
62941 attttctcta aaagggagtc aaacaaacac attcagcttt cccagaacct caaaggcagt
63001 gcatctatca gcccagaagg acaccaagaa cttgcctttg gctgacgacg gagtccgtgg
63061 tgtcccgatg taactgaccc ctgctccaaa cgtgacatca ctgatgctct ctgcataaag
63121 aggagacacc actgagtgaa agccgcgtgg tctatcggag gtctctgttc tagttgtccg
63181 'tgggacgtag gcagggtgtc cacatcagtg tcccagcggc ggccctcaga cctccactgg
63241 accctgtccc agggcctggc agtgttgagg gcccctcctg gggatgctcc tttccgtctc
63301 tctgaccccc ttctctgacc cgcagccctc aggcactcgg ctggcccctg ggcctcactg
63361 ttcacgcctc tggcttccct cccgccgtct tccatcattg cccctgtccc ggtgaccgcc
63421 ctgtcccact tactacacct cctactgtgg ctcacccaga ttccctgagg acacttcaac
63481 tccttgcctc tgcacctcca cgcgtgcctg ctggtttcca gccagtgact ggagcatgcg
63541 cgctggaaat atggcaccgt gagcagctcc tccacatggR atcagcagcc aagtcctgtc 63601 ccttctacct ccagtcgccg cctcatcctg tcccgtcgtg ccacactggg agcggctcag
63661 tccacctccc ccctcgccct tgattgttca tggcacctac ccgcaatgaa Mcccctccag
63721 tttgccctca ggaacaggag aacacagttg agcagattcc accgcagctt gggtgtgaat
63781 tctggctcag tgtctcacca gctgtgtgag ctgctctgag accctcaggt ttcttcatct
63841 cttaagtggg tataaaaaca tagctacctc attggggggc tgccaggact acatgaggtt
63901 atccacgttg agtgcttagc atcgtgctgg ctgcaagggc agtgctcaag
[0383] Following is a genomic nucleotide sequence of a GLB region (SEQ ID NO: 9).
>7:41819501-41919150
1 taaccaggat tgctaaatta attaattaga gtactatcta attctatgtc tcaaccattt
61 gtgagctggc ttagttcaaa caataaatga tcaggtaatt cacctaaaat tcatatgttg
121 gcttactttt attccctact tactgacatc agtccaagtt gatcattagc agtaagaaat
181 attttatgtt aatcatgttt acaaaagtct gtgcttgtac cttcacRtgc aaaaacagac
241 atgaggtgct tcatggaagg aagtgtcaaa acactttctg tcaagcaagg aactatccac
301 ggttaacaaa ggtgtatggt caaaataggg atctttcaga ttaagctaaa aatacttaat
361 ggaaaaacaa agaaatattc ttttgccaga tttcctctga tatctatgct ttccaatgtc 421 atctgcactt attttccatc agacgaaaag tcctgctgtt tctctctccc tcccatggac
481 tcctttctct tcagagttgt aggacaagac ttctgccatg actggtcttt ttatttttgt 541 tcatgttctg tacatgtgtc atacaaccag gtatgaagtc agttttcctc tagagaacat 601 tattacaagg aaaaggctgg ataaaaagat ttgccMtact tactgtctct acgaagtgga 661 tttcatcata actcaataac tgctatttct atataggggc tattgactaa tgtctgcaaa 721 cacaaaataa tctggggccc aataactggt tttgcaattc attgtgatat ttgacctgcc 781 tcttggtata ggcacagcat catgcacttt tattcttcct cataggcagt tggtactgaa 841 aatgcatcac tacaggtgca aacaagtgct gacattacat ttaaaaaaca cataatggat 9Ol tcaggaaaat acatacctga ttgcacttgc agataagtga ccataggagc cacttgctga 961 agagctgcta cgggaattat tgagaatcgt gaccaaggag ttgggagacg tccttatcat 1021 ggtctgaagg tcaaagctat gatcggagag tggtgatatg gacagtgtac gttttcggct 1081 cggcctggct gacagcctgg ggctggagaa tctggtgcct gttatataaa caaaaaagaa 1141 cctaattacc tgcagttgga ctctatacca gctatttatt gctacttgcc tacgtggaag 1201 aagtgaagga taagaagctg gcaacttgcg gtgacaagca cctctcctcc cccatgtgat 1261 ctactagcta cagttgagga aactagggag aaatatttgt cttccagggg cttacctcgg 1321 gRgagctctg tttattaatg tgcagggaaa atgataaatt gctaaacaaa agggagagat 1381 ttttatgtgt tatccctttc atttctggtg atttatgaat ctgacggctg cttagatatt 1441 cccatcatta attcaccaca agcgacaaag acagtggttc ccacaatggc aacataccac 1501 cctccccacc ccaacacaca Scattttaag tcatgagcca aaggaaggaa atgaattaaa 1561 gtatccKaag attaaaaatt cagctcagtt atgccctcag tgcccagtga tttctagagt 1621 tacctgctct atggtatact taaagcatta ctaaaacaac ttctacataa ttaccctgaa 1681 tcagtgcttc tcaactgaga gcaattttgc cccccaaggg acacctgaca acatctgaag 1741 acattctggt tttcgggact cagcagatgc tactggaatc tagagggtag agtctaagaa 1801 tgctgcagaa caccctataa tgttcagggt caYcctcccc acaataaaga attatctggc 1861 ccaaatgtca ccgaggttga ggacctctgc cttacattcc ccatataaga ggataaatcc 1921 caagaaacat ttaaaaggtg gggatgttgc ttgtttacag attttccaag gcagaggaga 1981 aacccttacg agcatgataa cgaggtacaa tgaacctata gggcatttta gctcttctct 2041 ggaccttgtt tctctagtca gacccaataa ggggtaataa atttattaga atttttaacc 2101 ttcaagaaga ttccactgat ggagtcttaa cctggagtcc aaatccggag gtgagtaact 2161 caaacttaaa tgcatgccaa cttaatacaa tggtttcttc ctgaagagtt caaaattaaa 2221 tgccaatttg cattcaaatt tggtagatat tagagatgtg agcttcaaga cacagagatg 2281 aatgtggccc acaggtccat tctgcctttt tgaaggtcat atttacatta cagagtcatc 2341 aaactaaagt ttaatgaacg aatgagaaca taatctcacc aSgtaggagg caaacaaaac 2401 atgattagtt ttaagtaaaa tgaagagttt ctaacacctc atagaatgag tataccagtt 2461 tataggaaaa tggcaatttt gcggtaccca gaacaactga agtagcaggt aatctttctt 2521 tatttaaaaa aaaatgtcca ggctcttggt ctccagaaaa ttctttggga atattttatt 2581 caataaagat ggttcttgat acaagataac aatccaacat gcttagagat catttaatta 2641 aatgggatag ataaatatga atgacttcat tgtcaatact atagaaataa tgaaaaaaaa 2701 tccacaaact gaaatttact aagttagtgc catccacgta taaaacttaa aaaccaactt 2761 gaatgataaa tgtggtacct actcaaattt ttctttcagg cacaactaaa acgagttcct 2821 tcatttagaa tagtttcatt accagcctga tttagagcat gttagcacaa aatagcaatc 2881 ccaacgattt cctttttttt tttttttttt tgagatgtgg tcttgctctg ttgccaggct 2941 ggagtgcagt ggcacgatct cggctcactg caactctgtc tcccgagttc aagcgattct 3001 tctgccttag cctcccgagt agctgggact acagacacac accaccacgc ccggataatt 3061 tttttttatt tttagcagag acgggggttt cactgtgttg gccaggatgg tctcgatctc 3121 ttgaccttgt gatccgccag tctcggcctc ccaaagtgct gggattacag gggtgagcca 3181 ctgcgcccac ctgtgcatat ttttaaggca tttaaatcag catcttactt ttataatggc 3241 atctgcctta ttaaacacaa ctccttcaca tttactcacc atgagaggct aatttcatca 3301 tggaagtttt tcaatgagga aatacatgtc acttccaagt gacaaaccag atttgttatc 3361 aacttccata taatcctgga caataattgc ccYtctcaat ggtactcagt gatcactggc 3421 aaagttagaa gtgacagcac atgctagaca ggacgtggat atatccctag aaaatctcta 3481 atgttttaga tcactaattg tgatgcaaaa ggaagaagcc catttgatgc tctcaaaccc 3541 taggagcctt agatcactct cacatcctcc aaagagggtg aggggcctct caagaccttt 3601 tcaccctttc catgaaaccc tttggcttgc tcatagcctg gctggaacct ttcctctctg 3661 tgtttttccc tccaagactg gctcttattt aagtctattt ccaccaatat aaatagtggt 3721 cttcaggggc cctcatctct agaatgttcc ttatagggag tatttgttct catcttttaa 3781 ggtcactcca acactgagag gtagaattgg gaccataaac agactagttt atcctaacaa 3841 gctacatata aacactctgt cctacatctt gctgaaacct agacgtgccc actatttaaa 3901 agtcaggtgc ttacctgaac ccagtttatt gagggttgtg ttaattaaga agtcctaaat 3961 atctgtaaca agggatgaag agtgaccaga tagatcccag gtttcgatct accactgcag 4021 atggacaacc actataaaaa tttaggatta atgtttcgct tctttgttgg aaaaaatctc 4081 ctatggtgga aatttctggg caatcttctt atattctaac taaataacta ttaataaatg 4141 ctacttcaat tactttagag gaaaagaata tagtgcctta agaactttcc agccctttaa 4201 tatgttcttc ccaactcctt aataaacaag caaaagaaga gaaagtcagc atttggggac 4261 agtgatttgg gatactctgt tgaagggagc tgctaaaata caaggtaagc attttcagaa 4321 cagccaaaag agaagtagcc cagttcttca ctgatcatac cacttccttc ttaaatataa 4381 gccagagaat tcttattgtt ctaacacaga tatctccgat ctggctttct gttaattatt 4441 ttgtttcYct attctaataa gaaattctga caaagaaaaa agaggtttat ttttatgtga 4501 tgaatgatag gacatctaaa ttcctactta atgacaatca atttaccata tactcaggca 4561 aattaaaatt cctttaatta gtttttacaa agtaaaaggg tagcttcatc ctttatttag 4621 ataaaaaccg ccactgcctg actaaacaca ttctcatcaa atagggaaag taatgcgggc 4681 tgaccttcca aggattctct cttattttaa ttctttattt gcagatttta aataactgat 4741 ttgcataata acacaacttt tttttcaaca atcgctaatt gaaatgtggt tatgatgaat 4801 ctgaaatgta agctctctat gctaaaaagc caaacaacag aacgcctgaa gaagacgtaa 4861 cacatttctt ttactttcca tgtagtaatg agcttcccag tggaaaccaa cagaatcttg 4921 tgttccaatg aaacacacaa aggtagctct tgatggtaag cagactaagg ctgtagagtt 4981 tttacccctg tatgaaataa tcaccttctt tctagtccga gactcaggac taaataacta 5041 gaggacatat gctcgYcctc tgtcacagaa gaatccaaat agattgtaaa tggaaaagtg 5101 taagttaatt gctgctacta ggaaaaacaa acaaaacgct aagcaaaggc cctactcaaa 5161 gtcatactgt tgtcctattc aaaggtagtg gcttttgctg atgtatgact tggatgatgc 5221 tttaggatca cttcagagcg ccttgcaaac ggatgtgtcc ttaaaggatc tgaagagatg 5281 agcgctctgg aatgctctga tgagaggtaa ttataggctt acttgtctat gcgtctacat 5341 aagtgaccca caaaaatcat tacacaccta agggacccca aaataagtga tacaacatgc 5401 atcaagtaat ctcaccttat tccactgcta ggtactaaaa gtagtccctt ctgatttgga 5461 aaaagttttt attttttcca aataagctat ttggcaacta ttgataactt gataactatt 5521 ctgatccttt catatattca gcagctacaa acKgtcttag tggaatacaa tttttttaat 5581 gtaaagaaaa catatggggc aaacaatcat ttaaggcaaa aatgtaaaac ctgttaaaac 5641 aggtattttt taaaattaat ttttaaagca taaaatccat gaaaccaagg agtggggagg 5701 caaagtccat ccatatatca aatccattcc cccttatttt gttatcaaga caatcacgac 5761 aggaaaaaat ttttttggtt' tggggagaca gggtctcact ctgtcaccca ggctggagtg 5821 cagtggcgtg accaagactc actgcaacac cgacctcctg ggctcaagtg atccttcagc 5881 ctcagcctcc tgagtagcag ggactacagg tacatgccac tacacccagc taatttttaa 5941 ttttttgtag agatagatct cgctatgtca cctgggctgg gatttttttt aaccagctaa 6001 tatttatRta tcgctgtcaa ttacaattgc ctgcatctgt cagttcactg gtatccttga 6061 gctaaacagg gggtctcagg atgtccaaat ctgtggaagc ttctagaaRa cgaagggaac 6121 cagagcacca gatagtggtg gttccacttt ctcctccaga tcaaaaagtt acacaatcac 6181 ctaatctttg tatcttcttc tctgtttcat aaagttgcct ttgccatttc ccaagaotct 6241 agaaaccagc cccgtcactt actatccata gcatgaagat attccatgtg gatggccccc 6301 gtgccggcgg tggcagctga gggaataatg tctgcatagg ggctgcgctg gccagttagc 6361 agggccatct gatgatagta ttctgctggg ctgactcctg catggggcgc taggaggaga 6421 caagagatgt atggttacta gcgaaagaag gtcaactaga agtttctctt gaggcatctc 6481 agagtccatt acacaaccca catcagcaaY tccttttatg gcttattaga gtaaagctcc 6541 tttaactcaa attgtgctct tacttccatg aaacatcttt tatttgaaaa taaaactgaa 6601 ggaaaggaat agaaaaaagt gaaatgcatt actaagtttt tgttgttggt ctaagaaatt 6661 atcaacaaat cagaatgatt aaaatgtata tatgtacata accacatttg ccccccaaat 6721 aaaatcattt ttatatcaaa tcactgtatg tttgcatttc ttacaatagt agacaactta 6781 gaagactatg atatctttcc agacatactt aaaatacttt tctaagcttt ctgacaagag 6841 tgtctgagtt aactctcact cacatgaaaa gtcagggttt accagtatga aaaaaggcag 6901 cacagcttta ataacacaac tgaatgataa gctacaattt tacattccat aatttaaaag 6961 ctctcttttc cttttcttca ttacaaaatc aaaaagaaaa tatacctatc aatctggagc 7021 aatttactgc caagactcag ctttctaaca ggataatagt cggtttccac ctgccaattt 7081 tcgtgcaaaa gcagcaagac attgtagcag ttctgagggt ggcctccaga ctcatagaga 7141 tctggattta aactggctcc atgaggtcgt caggagcaaa aattaaatta tgtatgtagc 7201 acacttagca catagcattc aatcaataaa agttttacga ttattatcta gaaataccag
7261 cactctagtg ataggtttgt gggtatttta tatttgtttc atatactttt tatccatgat
7321 acatggtaaa gaYWgagatt taaggaaaaa aactttaggc cacaagggaa atatttatca
7381 tgttacttta tttgattata ttaactggat tcttgagagg caagaaaagg aaggcaaatg
7441 aaccagtcaa agaatgtgat atatatactc gtcatcaaat gctacacaaa gcagatgata
7501 agagtgcttt cagtagttta actcgtggta tgactttcca ctagcccgaa taaatttctg
7561 caggccaggc ataattccaa gtccattgtc agctagcctc cctctctccc tcagtctatt
7621 ctccttgtta gtcatttcca ccaactccca aggacttgaa ggagatgatt ctccagttct
7681 tcaagtgcaa aataaaatct gaagcagaaa atctaattca tatttgattt ttRtttttaa
7741 aatgtgaatc ttggccaggc acagtggctc atgcctgtaa tcccagcact ttgggaggta
7801 gaggtgggta gatcgcttga gctcaggagt ttgagaccag catgggcaac atggcgaaac
7861 gctgtctota caaaaaatat aaaaattagc taggcgtggt ggtgcRcacc agtggtccca
7921 gctacttagg gggctgagac aggaagatga cttgaaccca gcaggtctgg ggtacagtga
7981 gctgagattg caccaaggca ctccagcctg gggaacaaag tgagaccctt tctcaaaata
8041 aaataaaatg ttaatcctgc ccaaagggca aaacttattc tttagctacc tgtattacta
8101 ttattaacca gtaaattcaa tgcactgaat caaaaaagaa aaaagtggct gttactgaag
8161 gaaagctatt tctgaaaacc caagtgagtg gatgtttcct gagacacttc atgcagcttc
8221 tcaccatgca cacccagcct caggaaggcg tctacctaga gaatgtggat agggcatgga
8281 agtcgcatca agcactgctc agtgaaatga aatgcctggt gtttctctga tgtggaacag
8341 gcagtggtga tttgcgatct cttgcaaaga attcagagcc tgctacctgt aattaatagg
8401 tgatgcaatt ctggaccttg attcagattc cagtgattat tgggcaagtg aattcctatg
8461 tgtgcgtgat atggaaaata cttttcctga atcaaaggac cagtttataa gttgacacca
8521 ggagaataca cagctccaat taagaagagt catgtggaaa gtaatggggg gactaacaga
8581 accaacagga attcgatcct taagagccta aaatgagcaa caagtataag aaagtctaag
8641 ctcatttcct ttcccagtac atacttcttY ggattttcat gtggcatggt caagaataga
8701 aagcacaggc aacagccctg gaccatgatg ttttctaaga ccttgccact cagagtgtgg
8761 tccaggcacc aacagcactg acatcatcgg cttttgagga gcacagaatc tcaggcccca
8821 cctgatccca cctgatgcag aatcRcSttt tatcaggctc cccaggtgat tcatagacac
8881 actaKagttt gggaagccct cctctaacag acgcatStgt ataaatgtgc tcagacacac
8941 agaacagaaa agtgttcatc agaaggtgag taaaacactc atgttacaca tgcacgtgtg
9001 tgaatggcta caggaccagt ctaatcatat gtgaagcagc agaacagaac cctagttacc
9061 agcagggatt tgggaatcag aaacattttc aatgaattcc acctctgccc cttactagcc
9121 atgggattga ctgcgttgca agtaaacccc actgcacgct gctattctga catgaaaatg
9181 gagataataa caccactgcc aatgaggtgt tacaaaactg aaacgcaatc atgcatgtaa
9241 ggcacRgagc acagcgcctg gcacatggga aacactcact aaacatcagg tctactttat
9301 acacgtcccg agtgaggagc ggggaggagg ctgcatgatc tccagaagca gaatccatcc
9361 tggacttacc atctgtaggg ctcagcccac gggttgctga gatcatggag agcgatgggc
9421 tgctgtgcaa ggagcggata tagtccatgt agggattaat gtagggatgt ggagggctga
9481 agggagactc ggaagcagca gYggggttcc gRtgtgggga gatcctaatg aagggcaggt
9541 ccggatacgt agggctacta gataaggcgg aagtcctggg tacaaagaaa accagataca
9601 aggggtatgc atgagacaaa tatctccaca ggcagaggct Rtctctgaaa gaaaataagt
9661 aagattttaa gtgaattcaa aggagccttg tggaaaaaac tgcaggcaaa tacatattag
9721 tactattatt ttaaaatcag catgatataM tgcaggcaga gcatcgctat gcaattgttt
9781 gggaaaataa attgatctct gagaaacttc attgcactcc aaaatagtaa aataaaggtc
9841 aacctatggc ttcttccatg agaagaaatt ctaaataaca ggcaactggg cttaaagtgg
9901 ttgctacaat tgtattttcc acttgaccca aatattaatg ttaccattta aaacttacct
9961 cttactagca tggtgttgca taaaaataag aaagaaaaga aaagaaaaaa gaaagggaaa
10021 gaaaagaaca gaacagaaca gaatgaaaga atacaatccc aaatttgtct ggtctggaag
10081 cctgatcatg cacatggcag agctgtccgt aaggaagaag acccattgca aaagtctgct
10141 tagaaatggg aaatcggtca ctctaaagat ccaggcacat gtctgtttaa cttcccaggg
10201 cctcgcagYg gtaagtaaaa tgacttttat ggatccaggt gtgagtgctt ggaaggcaac
10261 tcctcccctt tgtgcagcag cacctggaat cagctttctg ctcaagccct tttccatgag
10321 gcatcccgat gagccacgtt cagtgagcag tagcaactaa gtgctcccgg gttagaaagt
10381 tggggaagaa tgcattttca atcaagccct tgcgtcggtt ataatgagct aaggtagaat
10441 gtaggctttt ggttaataaa aataaattct aaaacacacc ttgctaccac gatatcctga
10501 tctgcttctg gagagagatt cctcaaaatc ccctcacacc tgctgcccca tagccacagc
10561 cacaccctga gacagccact tctagaatgg ccagtctgca taacctcctg gaccaccaag
10621 ttccttctct aacggaaaac atttggcatg gataatcagg gaactggctc tgacaggtta
10681 aacagttgtg ctccccgctt ttcctcattc acagagggtg cggccatagg aaggggaggc
10741 cctgatagag agagatcaac tgagaacagc aaattcctct tccaccactg gctttaggag
10801 tgaaatgaaa gggaggggga aacacatttt ctccttgcaa agcaaatgct cacgcacagc
10861 ccggccccca tcaccctgtc agaatataat cttggtaaaa gccagctcca ctaagtgcac
10921 agcgcagaca ctgaaataaa tgaaatcaga gatcagataa tgaattttct ctcagtagaa
10981 aatgaaagaa gttgaaatat ggtggtgggg gtggattttc tcactttctc cttctgactc
11041 ttttttcatt ttgaactaaa aaaaaaatga ggctttttat ttgttactta gatgctactt
11101 tgggaaagca aaataaaaat ccaaagtttt ctaaatttac acatgggaag agtaagaaat 11161 caccaccagc aaatagctat ttcttttcaa aacagcttga ggctttcaca cccaatcaga
11221 aattagtaaa atggcttgtt taaatagaaa tcaacataac ctattagtct ttcctatttt
11281 agctctttac tacaaaacaa gttggctttc tgttctttgt taactcagcc cctaagctcc
11341 atttagacgt gtccgccatt ggttaaatct tacaccgtat ttagatgtgt ttgctacctt
11401 agaaggtaca ggggatgctc taattcattc atttattctt cacaagaaag gacaagcaat
11461 ccttccaatc tgattctaaa cacagacttg tttatagatt tccactgagg gactggtgcc
11521 accgaatgca gaagagctgt ttcaagaaag ccatgagatg acgcctcctg ggccagcttt
11581 tgcaatccag gaataacttg gtctgagcaa catgaactaa acatcagggt gggcagtgac
11641 atttcttact ggcttttctt gatttaccca aagattgaaa cctattattt ctccaaaatt
11701 ccagagttga gaagagtggt aagaatatcc ctagaggtcc aaattctatt ttggagaatt
11761 atgcacagga aaaccaatca gcatctagaa aaacacaaaa attatagMgg ccaggctagg
11821 gtggtcatca atcccaaaga aagcctagga agctgttttc tcctcacaaa agtctatcaa
11881 gtctgatgtc cctgtcactg cttcacttgg attcaagcct ttcccaccag gacactgaag
11941 gtacctacta aaccagcttc ccacgagtga ggctccaaga cctggtcata tcttctctgg
12001 caggagcact gggctctacg gtccttttac atactgctga gaagcaacag tggaaaggag
12061 ggttcctcca ggatttgaag ataaagatac aggtgcatca ggcagtcata agggggtcat
12121 caggaattct agatacagat gtatccaagc agtgggactc aatgtgcgtg gacctcagcc
12181 ttgatactca ccttctcctc ttccttaaag gtccaatggg gaagataacc tcgtgggagt
12241 cttaggaagt tgacaagagt aaagtctcat gtactgagcc tagtatatac tgaatgctca
12301 acacatacac acacacacat atatacaaat atacacatac atccatatgt aaaatcatat
12361 gtgacatata catcacatac tgtgYtaaca tattccaccc ataatacaca tatacacaaa
12421 catatttata tacacatgtg tatatgtaca cagatatatg cacacatata aacatatata
12481 catgcatagt aggggtagca aatgcaacat actgtgtgat ttcaaatatt gaaatattaa
12541 gacattcttt ttgttagcag actttgttta gagcaattta aattcatagc aaaattgagc
12601 tgtagtacag agatttccca tataaccccc acccccagcc tctacatata aatagcctcc
12661 tgtcattatc aacatcttct accagagtgg tgcatttgtt acgagtgatg agcctacact
12721 cacacatcat cgtcacccaa agtccatagt ttagggttca ctcttggtgt tatcctttct
12781 acgggtttgg gcaaatgtat ctaccgttac agtatcatac agagcagtct cactgcctta
12841 aaaatcctct ttcaccaatt cttcccttct tccttctccc agcccccaac cccaggaaac
12901 cactcatctt tttgccctcg ccatagtttt gccttttcca gaatgtcagg cagtatgtag
12961 actttccaga ttggcctctt ttacttcgta atatgcattt aagtttcctc catatttttt
13021 caaagttcat ttccttttag cactgaataa tattccattg tatggaatag cttatttact
13081 ctttcactta ctgaaggaaa acttggttat ttcgaagttt tggcaattat gaataaagct
13141 gctataaaca tctgtgtcca ggttttttgt gtggaagaat cccctttcca tagcaaatct
13201 gatcacacca gtcggctttg ctcctctccc tgacagcacc ttggaggcac ctccttggat
13261 tttccaggac ctcccttagt aaaatcataa agtcacatga ggtggttaag aaaatgaagt
13321 cagtaattaa atgcaagggc tttgaaggaa tgggatttga aggtttcttc acttattccc
13381 tcactcaagc atcagtttcc ttatacacaa aattggaaca atcacagctc cctccaggag
13441 ctgccgtgag ggctgaaatc ctgtgtgtaa aggtaggaac acagtgccta agtgctgtgg
13501 ttgttgatat tcttgtagaa ccagccaaca acacttcttt tcttctgatt ctaaactcag
13561 aaaaaaaaaa gtccaaatgg cgatttgttc cctagcggag cagtggctgg tattcagttt
13621 ctcattaaaa tgtggtcact tattaattgt ttccacatta ggctctcgtg aRctgggtta
13681 gcatatttct ctaatgctaa agtgtgacaa aaatgagttt ctaagcaaac aaagctgagc
13741 atccaaactt caactaccat agccaaacag cctcctgaca tgtaaatagt ctttaaataa
13801 aggtgttttt aaccttacct aacggagcca tcttcaaatt cttggcagct agatcatttg
13861 tcagttattg tggctttgtt ttttgttttc gagacagagt ttcactcttg ttgcccgggc
13921 tggagtgcag tggcgcaatc tcggctcact gcaacctccg ccctccaggg ttcaagcgat
13981 tctcctgtct cagcctcccg agtagctggg attacaggca tgtgccacca tgcccagcta
14041 agtttttgtc tttttagtag agacaaggtt tcaccatgtt gattaggcca gtcttgaact
14101 cctgatctca gatgatccgc ccgcctcggc ctcccaaagt gctgggatta caggcgtgag
14161 ccatggctcc cggcccaatt attgtgtttt taactggaaa gcaaatctgg gagagaaggt
14221 taagctcctt aaatttatca ggcagacgtg tgttcactgc tatgtgaggg gcactcagga
14281 ttatgaccag aggagggctg aattccactg aaggaagagg acaggggagg tatggagaag
14341 gcggccacaa aatttcccat agaaatgaca tatattttcc tacaaactat catcaacttg
14401 gcctggtaaa tatttgtcga gcttgttaaa aatcttcatt tgaagattaa aagtagtgcc
14461 aggaaaaaaa ctgtggtgtc tgcttgatgc acatttttag tagatacaca cagaaatgaa
14521 ctgaggatca ccgctttcgg tctttgtggc agtccacctt ctctctgtcc tgggactgtc
14581 tgtggctcag tcattggagg gagacacaaa ccagggagga aagaacaaca aacaaacatt
14641 ctgagaaaaa cactgaaatt cagaactggt ccaagaacag acattccatg ctatttatgt
14701 tgcagcaaaa gagtatagaa atatgcctgt ggccctccaa actaaatctg acaaactgct
14761 gtctgggcac cagagattcc caaactgtag agtgttgttg ggactacagg gcacagctgg
14821 gaagatgtgc ctcgccccat tatccctgtg tcttggctcc tgtatgcaat gcctctgtgt
14881 cctctcaggg ataaggccct ttatgctgtt tctacccctc tgtcttccaa gacattggag
14941 acctaatgag tcactggcat tttgtttccg tattgcccag tggaaactgt ttgttggtgt
15001 aggtaacttg atttcagaat attttttata tggtcatgaa aatagattac aatgaaagag
15061 tagaagattc ttatcagagc atttttaaca gtacaccatg agaaacttaa aacatgttgc 15121 agagaataac tggacttctg agttagaatg aaatattatt atataatcta attcattact 15181 gccttttcat ctatcttact aggttactaa aatgccaaga ttaaaatcac agtatttaat 15241 cacttgcatt acccattatg agagcattaa acaaggttta catgccagat tattacaatt 15301 tgctcgcaca ttgcttggga tactggaaat gtacacatat ctcctgcatc tctgcatgac 15361 tgctccaact actggaagac tgaaatatgt tggtttttta cataagctta ttttttgttt 15421 ttttaaatat aacaagtaga catccttctt catattcaag cagtggtctg atagagaaca 15481 gagggtagtt gttcttttta atgcattttt aaccaattaa ttaattattc aataaatacc 15541 attttaataa atcaaaattt gtaagaaaaa atttgctttt gtcccagtgc agtggctcct 15601 gcctttaata tcagcacttt gggaggctga agcagaagga tcactcaagc ctaggagttc 15661 aagatcaatc tgagcaacat ggggagacct catctctaga aaaataaaaa taaaaaaatt 15721 agactggcat ggtggcacac tcctacagtc ccagtcccag ctactcggga ggctgaggca 15781 ggaggatcac ttgagcctgg gaggttgagg ctgcagtggg ccatgagtgc accactgcac 15841 tccagcctgg gcaagagagt gagaccttgc ctcaaatata tatatgtata cctatataca 15901 tatacacata tatgtataca tatatatata cacacacata tatgtataca tatatatgta 15961 tatatacata tatacacata tatgtatata tacacatata tatacacata tatgtttttc 16021 ttcctttcca tacactaaag aaaacaaata tctaaaatgt tcaagagtaa aacagtagac 16081 actgtcataa atctgcacaa tcctatactt ttttttccct ctatgcaagg taagctttca 16141 gataacctct gccctagaaa caccacgtga ccctgagagg tgcagatggt tatggtggtt 16201 actttccatc tgctcttcct cctccccaag gccacagcct gtgtggggag tctcgtcttt 16261 aaggtctgaa tcagctggct cgccttccct ccggtttcca cttaagggaa gcaaggcagg 16321 agatggcagg aaRggaaaac aagtcccctc ctccctctct gctagc'tgtg gctctgtggt 16381 atggcattgc tcccccaagg ccactgttcc tggtgggcga gccctcctct gctgctccag 16441 ccctcactga atcacttttt cattccaact ttgactgtat taggggcttt actattactt 16501 gtttgtcccc ttaaccctac ccacacctct gtacataccc actccatcag cccccaggcc 16561 cctagttgat aaaatggttc catttccaat cctattttca tcatctttgt agctatgaaa 16621 tatttttcca tgaattctta attggcatat gctttttaca aatttaccat ttggatttta 16681 atttttaaaa actacatgta taaccacaca gatgaacatt tcaatgatct cttcattgga 16741 tatacatttg atatcatttg gctgtgtctc tacccaaatc tcatcttgaa ttcccatgtg 16801 ttgttggggg gaacatgtgg gaggtggttg aatcatggag gcgggtctgt tctcctgatg 16861 ttgaatgggt ctcatgagat ctgatggttt tatagagggg agtttccctg cacgggctct 16921 ctcctcttgt ctgccaccat gtgagaagtg cctttcatct tccaccatga ttgtgaggcc 16981 tccccagcca cgtggaactg taagtccatt aaacttcttt cttttgtaaa ttgcccagtc 17041 tcgggcatgt ctttatcagc agcatgaaaa tggactaata taacatttta ttgaaaatcc 17101 attccctaag aatccRctat gatttctgca cttgggagag atttcaagga gcatgataca 17161 tggttgcatg gtctcttctc tgaaagagcc tacagtcaaa tgaggcaaaa ggctacactc 17221 aaaagaagtg tgtatgagta gatgtgacct aatgagtggt ggcacagcta aaaagcattc 17281 acaggaggct cccaaaagca tgtgggtcat gaaaaatatg gacaatgaac cagcacaaaa 17341 agtaaatatg atttgaatga aagtggccac taaaggtgag ggctagaata ttgcttaacg 17401 tatgcattaa tgctaaggtg atgaatattt tcttacatgg agaatttaaa ggctaaggct 17461 gattttttta aagacagaga agggaaaaga aatatttgtt ttaatgatga aacattactt 17521 caagagattt ttgtaaataa gtttaYtggc cgggcatagt ggctcgtgcc tataatccca 17581 gcactttggg aggctgagtc gggtggatca cctgaggtgg agagtttgag accagtgtga 17641 ccaacatgga gaaaccccgt ctctactaaa aatacaaaaa taaataaata aataaataaa 17701 taaataaaaa attagccggg catggtggtg tatgcctgta atcccagcta cttgcaaggc 17761 tgaggctgca gaatcgcttg aacccaggag gcggaggttg tggtgagctg agatcacgcc 17821 attgcactcc agccagggca ataagagcga aactccgtct caaaaaaaaa aaaaaaaagt 17881 ctcttattct gagtataaaa ggacttaata ttccatgaat gaaaaagaca agtggttatt 17941 taagaaaaga aatagccatt cactctatcc aagctaacca atgtcaacat tttaacataa 18001 ttacttccag cattttctca ggcattatgc acttcttaca tattaaaaat aaaaatcatg 18061 tattttgaat tatactttat ttcttagtat gataaggttt ttctattatc ttttaactaa 18121 ctctccataa acatcattta tagtgcagtg caaattaaaa ccaaaatgag ttatcattaa 18181 acacccacta gagtggctaa aattaagaag tctgacagtg acaagtgttg atgaggattt 18241 aggccaacag gaactctcaa tRtgagcgca agctagaact acctctttgg aaaacaatca 18301 ggcattgggt acttgcacaa attcttacca tatattccag caatcctttt ctttggaata 18361 tatgagacaa tcacgtgtgc atatgtgcca catgtactca aagagtcata gcagcattag 18421 tcatccactt aaaaattgga aacaacctaa tatctatcaa cagtagaatg gacaaataat 18481 gcatacaatg aaaYagtaaa cagctatgaa aatgaacaaa ctacaacata aaaaatctca 18541 caaacataat gatggataga agaagcccag cacccataaa aataatgaaa taatttcttt 18601 tgaaactact cggatggagc tagaggccat tattctaggt gaagtaactc aagaatggaa 18661 aaccaaatac catatgttct cacttataac tgggagctga gctatgggta tgcaaaggca 18721 tacagagtgg tataatgaac tttagagact gaaaggcagc agggggcgag gggtcagcgg 18781 aggggaataa aggataaaaa aactacatat tgggtacaat gtacactact ggtgtcacag 18841 gtgcactaaa atcttagact tcaccactat acacttcatc catgtaacca aaaaccactt 18901 gtaccccaaa agctattgaa ataaaaaata tgtaataaaa aagctttaaa aataaaaaat 18961 atgtgataaa taaaatggga agttcatcag aaaaaaatta aaattaacta aaaaaagaag 19021 aaaactggca caaaagaata taatgtatga tcccatttat ataaagttct ttaacaggca 19081 aaagtcaact tcagtgttag gatagtcagg ataatgttga ctttcaggtg ggtggagagt 19141 atggaagaag ggcttatgag gtgtggggat gttctatttc tagaactgtt aagttagaag 19201 ttttcactcc atgatcattc atcatcattt actgagctac atgtgtacat tttgtgcaac 19261 tttctctgta ttattcatca ataatttttt aaaaggatac atgccaaata catcgagatt 19321 tcccacagtg gtttcaacca tttcaattaa attcagaaaa tcaaattgat acacattttt 19381 atttctcaaa acttacaaaa tattgatgct ttaaaggtaa cataaataat aacatatYta 19441 ctgctataaa acaaaaatag tctagctgac acattagtct aaattcaagg taagctatgg 19501 aaggtggaca tctgtgtatt aactttggag ggggcagggg ctacYgctaa gccaatcaaa 19561 ggagcttctt gtgaagagag aagccaaaca aatacatgtt agtcatgttc ttccaacaac 19621 tgaaacaatg ttatacaagt atcactgtac aagagcagtg caattgttta tagctaaaat 19681 tgatttcatt ccttccagga aagactgcaa agaacaatgt gccaaatttg tctctactct 19741 gtactgtaaa tataactcat taataaatta aaatttcaag gtcaaagaat taaaaagaaa 19801 gtccattcca gccactRtaa catgcaatgc actgttatta aaaagagatc ttaatgaaca 19861 tactgctggt tcaaataagt acttgagagc taaatgacca catctcatag catgggactg 19921 cgttctatcc cactaacttt tgtttaaaga gagctcaatg tatgtttctc acattttaga 19981 attaagaaca gaagagaaaa agaagtggag aaaagagcgt acctgaacca aatggcagat 20041 gacaacttgt ttttaaataa catatgtttt aaatatttta attccatgaa tacacattat 20101 atattaaatt attattttag cagacttttt tgtcctccaa atggagtcca aatagttcaY 20161 gcaaaggtat ccttgtttct gcaatgtcta ataaatgaaa gacattttct tatcttcttc 20221 ccctgattac tagagttggt aagaaggata aaaccRgtaa ctattatata aaatgtttta 20281 ttatataatg tatgtattat ataaatataa agtggaatta ataagtataa atgaatataa 20341 tattcattta taattcttaa ttacaaatta taaattaatt aatataataa aaataaatat 20401 aattcattat tagaagaact aatatgctat gtccatcttt acaatgtcca cagaagtacc 20461 accttgatga ggaaacagct gtcgctgtca aaagagacat tcatatgtcc agttgcaatt 20521 cttttcctac cacctaggtg ccatgttgaa tgggcgatgg agagctgctc tcattggttg 20581 gattctgtga catctgatgg ggctctgttt tctagacagt ttcctaactg atctgcatta 20641 agaaagcaca cagatgctgt cctttcctct cctcttcccc taagataaca agtggttacc 20701 tttgatcagc caagcttttt cccttgggta gtcccttttc ctccagtgga gcaggccacg 20761 gcagaggcca atccagccag gcccagagga tcaaaggagt ctttccttct gtctcactct 20821 cctgctccct atacctggaa gtgtgcattc agtaagctgt tctatacacc agttgcaagg 20881 tcttgggctt1 aggtttgcct gttgggtgaa ctagatcctt aaaagcctgc tttgctctcc 20941 agctacttaa gcccagacag agaagcggca gcactgtacc atttcctttt taacagggaa 21001 gtatacagaa agcatgtagc ctggtgactg tcacatggta aattctcagt aaatgttggc 21061 ccctacaacc aacaccttca ttcctgctgc tgctgccatt cctagagctc tgggttggtt 21121 aaggccaaag tcacctggct tccgttgcct tgttgagtac tgatttccct caaggacctg 21181 atagggagtg agctgtttgt ggccgggtga gttcagctat gatgtcactt cggcattact 21241 taggcgcacc catctcatgt tttctcctaa cagatagatt aagcgatttg atttacctta 21301 gttaagatga tggatttttt ttcttcaaag taaattcaag agcataataa ttctgtagtt 21361 ttagttcagc aacttcattg ccatgaagaa aaaaaataaa aacttttgtc tttagagata 21421 gagaaaacaa aaatttttta atcagtttga tttctgtgac tttcctctct attttccatt 21481 aattctacca atcctatttt ctgtaatttg atgaattacc aattccatct caaaaacaat 21541 actcaaatag gcaaaataat atcctttcca aaagaaaata caaggcaaaa tagaaaaata 21601 ttgacaccca atagtgtaga aagaatggct cctcaaacaa ctaagtccct ctatgatgaa 21661 ctaactgtgc ctagaaaact cctgggataa gtgacatttg tttgYatagg tgttacattt 21721 tccaacaatg cagaaactta tccatcatga tttcctttag cagaaacttt tgagtctgag 21781 ccataataca catcaagtgt aagggaaaat gactctaaca tacatacaag agactcagtt 21841 agatctgttt acaaacaaac cagctcatgt ttgtatcatt tacatggaaa tctcaacaat 21901 tcaaggtaaa cagctacttt aaaagtctca gcagcactaa ggcagaatct gttattcaag 21961 caagaacttt cccctcagag gacttcaggt actaggtgga tataattaat tgaaaacagg 22021 aagttgggtt ccaactattt aatccttgct taatcttggg tctggtggca ggtgttatag 22081 aggatattta taacaaagac aggaagcacc tagaattctg agccaagaag acagtaaggc 22141 tacctcaaga atcagaatga atcttttatc aacagtagtc tcatcttcta gctagtataa 22201 agactttttc Yatattagta tcatcatcat cctcatcata attattatta ctgttaacta 22261 ctaactagtc tgttttttga gttatccctt gaaccttgac atctcactcc tacttcccct 22321 gattctttta atcaccttta ttttaagtta tatagagatg tccaacaagg aactatcatt 22381 agtgattcaa taactctcct ccagaataaa atcttcaagg tttgctaggg ccattggtcc 22441 cataaaggtg aaaaaagtga gcccttttga agtggcaggc tctttagatg taactggaag 22501 tagagcttgt aggactacta tatgagacta aggctttatg tcctaagcat acttctcaat 22561 atttcagaac tttatcccct tggagggtgt taacttttca aaattaaatt acatatggaa 22621 aaggagtata tgtagtgtac tgYagagtgt atgtagtgat ggggaacaat ttgtctatat 22681 tattcaacag aaaagcagca tcaacttcta cataagggga tacttttctt aggggtcttg 22741 ttatcatggc cttcgggaac tttgctcaag acRtagagaa cagcatcatg gaggactgaa 22801 aatgagtctg gctacttaac aaggagctac aaagtctcct tctgcagttc cttcttcaga 22861 gagaaagcaa gtcttccagt accataaagg aggagagatt catctgattg gaaatgtcta 22921 tgatgtgcaa gaattacttg ttcatattac ccttggaaaa cctgtgcaca ggaccatggt 22981 cttatcaaaa acagcttttt gagcctcact gtttcatctg tcaaatgaga agctttggag 23041 tagatgatct ccattctact cacaaggttt aaggccccat ttgttcctaa aatccagtgM 23101 tttctatgat tcaaaccata ttcccctgtc tgtcattgct tagctttgac catgttataa 23161 agtctgttgc ttgaatacac ttttgtttaa aatttagtga cccatcactt gagtatccta 23221 caaatgagaa ctcttttctt tttcctttct cRagaatagc atcacaatca ttgcagcaac 23281 ttatgtcctc tctgggccac tgtggtcttc aactttgata ctactcccag gagaaacgat 23341 agggtctggc tttatgctaa ctggatttgg atggtgattg attagaagaa agtgggacca 23401 cgtagccttg caaaaggttc agtactgagc cacaagcttc aacactaaag gagcttttac 23461 cagtgagttc acagctattN ttatattaac aacatttctc ccatccaggt ttgtgcattt 23521 tatctttatt ctttcaaatc ctgaaattgc ttggataaga gagaaggttt atatattata 23581 cacacacaca gacacacaca cacacacaca cacatttcta agagcagcct gaacttttag 23641 gagaaattga agaggctttg tgtcaagtac agctaaatcc agcctttcca tatcaaacct 23701 ctgctagaaa attactgtgc agatgataag gcagaaagat ataataaaaa gtgactctMc 23761 tcaatttaca ctgcaatatt ggtatgactg aatatttttc attcaggtgc ctgataaaat 23821 cgctaaactg tattgcagat aaataaagga ggcttctgtc aagaataaac accattaatc 23881 atgaagtctt accctctcag agcctgtgct atgcatctgc acaaatacac cagcataacc 23941 atcacacaac acatgcttgc ctcctttata aactgttcca agctgaagaa gYgctcgttt 24001 ttcattttgc aaggtttaSg cagctatagc attttgaaaa cattctcttt acatttttaa 24061 ctttacattc ttgcaactag gtccattatt gtaacattta ggaaaatgtg gctgggctct 24121 caggggaaaa aatcttgaca atgaatcaat tttttgacca aatacatacg tacacacaca 24181 aatacatatg acatactaca tatcctcaac tctaagatgt tactgatcta aaggtgcaaa 24241 attgtgttag ataccactaa aaaaagataa tggttcccta tcacttttgg tttttacttt 24301 acttattaaa agagctcttt tagaattatt cagacataga ttttttaaac atgtatcatt 24361 ccagcacatg tacaaaaaag ggaaatataa gcaaaattat tttattaaaa gatacctgga 24421 acttcctcat gactgccact tcaccaataa gcaattgtaa aacactatag attgtaaaat 24481 acatcccaac ttcagagatg ttaaaagatg gaaaaaggga tgtactactt agaatagatg 24541 atttaaatat ggggatagat atgtagtagg caatcctgcc attcgtcaaa tacctatgYt 24601 gtgacagaca ctgtgttaag tgctatatat atgctccttt gctgaatcct tgatataaaa 24661 tggKattgaa tggtgaaact ggcttagaca gattcagtaa tctgacagta agtatctgag 24721 ctgaattcaa acccagtcta tgcttcaaag tctatgtttt taaacccaaa gaaaaactgc 24781 cccaaaccag atacacatgt gcatgtatgc ctacacttac atttttaaca ttattgaggc 24841 ttttaataga aaaggatggt atggttatct agctacctta acttccaaag ggaatctatt 24901 taaattgatg ttcaaattgt agtgccatgg caggtcacac tggtggctgc agtatacaca 24961 aagtgtcact tgcagagtat aaagagtaat gagggaagta tgaccaccat tcaggcaaga 25021 cttttcagca gagcttcaca taaggaaagt gcaggcaaca aactttatgg cttagggaga 25081 aaaatggcat gtgggcatgc atgtgtcagg gccacaatat tttcatggaa tccccttgta 25141 agtgtccaca ctctaggaaa aaacaacgaa caaatttaaa tttttccata atcatgaaat 25201 gttaagaact agaaaggatc ttaaagatca tttaattaat ttattttgta catgaaaaca 25261 aggcctgcag agatgacgga aagtgctcaa ggatccggtt aacttgtggt agagttgaga 25321 atggaacctc actgaggtcc acatgtatta tgaaaacaga tgattaactt gttttacgaa 25381 gatgatcatg agaagccatg tgccaacagc actatgtttg agtttgctgg aattacactt 25441 tgaaacccaa ccaaatagca ttgtcatggg tgaatgtaac caacatatta ttgggtgggg 25501 aagggatgtg ctcaaaggaa ctattcagca aagccataat ccagtcacac tcatgaggga 25561 tgtttagctg tgttacgcag gaagtattat ggaagctgca attcagctca ctgggggttc 25621 tgtttgcaga gcccactcca agcaggaagt gccttttcta gccaaaagag gtttaactcc 25681 catcttcctg ataaatcctc cctctgtgaa gaagcagctt gatcctgtac agtgaagtgt 25741 ttatttgcta aatggaacac aggcagagag cctcacaata acgtctgtgc tccaatgatg 25801 cacagccata ttgggggctc acagaggtca gaccccaaga agcctcgttc aagccaggcc 25861 cagcaagaaa agaagaggat accaatgaaa ccaggccctg accccactgc ttggtacttc 25921 cccagttctt taaggaagag cttctgtgtt aaaaataagt atttatactg tatcattttc 25981 atcacagact gtgtgttcaa tcattcctga caaacttttt ggtcctcttt tttattattt 26041 ttattttata aaaattatat tttatttata atttattaaa attattattt taatattaat 26101 taaatattat tatttaatac ttttaaatta ttattaaaac tgctcccagg tacattatca 26161 ataaatgcag acccctggtt agctacaatg caccccacct gcttgttaag agtagaaatt 26221 ctcatgcaca aaaaagcaag taaaaacaac tgaRtgtacc cttcataaga attttgcttt 26281 taagacagct gaattttgtc aagctatatc acaatgtttt accactatct acattgaggg 26341 tataatgcat taaagaataa aatatttact ctttgttaat agagttaaaa acatagttca 26401 taagtttatc agctgttcat ttctgtccca gtcataaacc atgcttcaaa aaatagcata 26461 tgtacaRgaa aatgtagtgg ttatagtaag atactcgaat aattttgttt ttgttgttca 26521 ttatttctat acataaggca atccatttta tgcaaattat gatgaaattc tttagatatg 26581 taaatttaac tcaaatggag ctagtgggct cccctctcac cctacacggg atgatttatg 26641 ttactaactt gggcttgagt gtaacacctg aactaccctc cttcaatagc cttattcaca 26701 ctgaaagaaa accaaatgac ttagtagcct ctttcactga ttttaaggct agtaatgatt 26761 cgagtagcca tgttattaaa ctagaatgaa gcactactct cctcactaat gaggttaaaa 26821 aatgtcttta ggtctccacg ttaggggcac ttagcaagca ctctgcttgg cagagaccca 26881 cagcggatcg aagccatgga ctaatcacac aggccggaat caaatggact ggaagaatta 26941 ggagacttgt ggctcggagg aatgtaattc atgcagaaaa gcgttgtcag agcaaggcca 27001 gggaacctca tgggaagaaa gcaaaataaa cagttcaaca aagaaaacaa agaagggcca 27061 ataggctttt ctaaagcttg agtacactga gacttcagtg ggtgcctagg acaaagacct 27121 catcaaagga cacccttgca aagcaaacag accaaggaag gatgtttctc tccaatacac 27181 tcgggggaag ggggtggact ctacttttca ctccttccaa ttcaacttcc acaactccca 27241 acttccaact caactccctc ctccaacaaa cacagaaacc accaccaata ataacctaag 27301 taactaaaaa gtccWgtYtg tctgggttaa aagatgggga aaggtgggga ggttaacaca 27361 aattaaatat ggtgcagtgc atctctggat Ygcacttcag atcacgaaaa ggcctttgga 27421 agtgtaccca cctcctgggt cagatcccca cagcctgctc cccacattcc agaggatgaa 27481 gagagactcc agaagtgttc aaggatttaa actttgaaaa ttctctctca caaacagtat 27541 acaacatcca caaccgtgca gctattcaat tgcgagaatg gagcctagca tggctttctg 27601 aagaaggctg cacctctgct cacagctcca gcctcactct tctatttcca gtggactctt 27661 gacttggact attcttccct caatccaatg actacRtgta tcactgcagc cctgaaatga 27721 ctttggtcat taaaacaaga ttaaaatact tgaggctgac cttcttttcc ccctcttgac 27781 tacttccttt aagatggaag accatgctgt ctttggctga ctaatcccaa ggacaggctt 27841 agagtRctac aaggaacaca gacagaagtg agYgctgtRY gggtacatac gcatatacag 27901 taacatagtg aagatatata aaaaaacata aaatacttac tttaaataaa tcaatacaac 27961 atggcaggta ataaaagtat taacataatc caacataact ctaatattaa tttgttWtat 28021 ccctggcaaa taacatatga aaaatcagag gccccttcag aggtctgatt gcttacgtta 28081 tggacttaca attctaaatt tgttcctgaa aactttcctt agtgtgcata gtatatgtta 28141 acgacaatgg gtgctaggaa tatggggatt ttgctctgtg ggggaaccag agattacttt 28201 gaacagaggc aagaagcatt ttagtaaact ggaaatgttt atttccatgc tgtcttaaaa 28261 atgtaaagca tattcaagca acatggtttg gtgatttgtt ttgcaaatga ggcaattcat 28321 ttaactctag agcttccctc tccccactcc ccatcagatg atagagatac tattcactta 28381 tggtgaactt ggcaatattt ggatactatt tgaatgtatt tagaagaata ttggcagttt 28441 tcctgtttca tgtgtaactt gttcccaggt tttgaaagac atattaacct tgtcaggcct 28501 gagggaatca tttactgtgc ccagttaacc taactccctt aggctggcta cctaggccat 28561 actagacttg gccatggtat gcacagggct ccttaggaac aatttacagg tgYttccagg 28621 caatgaggga ccctgtatgg gggaacctta aaaaaaaagc cttaaagaga atgtcttcaa 28681 gggcaactgt acacacaatt cattttggta ttagttcaga ttttcagtct ggggcYaggc 28741 ttccattccc tgtaagagtc ccaactgaga actttttctt ttagtgaatc tgtaacctcc 28801 ctgtattgca taagaaaatt ttaaatatct gtcacttata aacatgtgtg atgctgcaca 28861 tgccaaaata ttaggtgatg ggatttttaa aaacctatgg ctttgggtta gacactcact 28921 tttatcttta ttggaaatgc actcacatgc tctaaggoag ttcctgccac tctgaccatt 28981 ctccctgcat tattttcttc ctgcacacgt gtgtagacac acacaagaga aaagaggcag 29041 agtatatttc tagcatcgca cttgatgttt ttcaacacag agttataaaa attgaggcaa 29101 tgtcaccaaa ttagctaagt cagcacagga ggtaaacaca ggccagtaag ccgtttaact 29161 tgttaatttt tactgaactg caacacaatc atgcccattt ctgctctggt ctgccttttg 29221 caaagtccac atcaaaacta aaaatggcca agattgtatt tttcaaattt gtttcagtat 29281 agctctctgc caaaacatcc ctagttttct cagagctcca aggatgagtt tcaaaatcca 29341 cctctctcct cctatttcct tttagtgtgc ttagcttgct tgggtcactg aagatccaaa 29401 atctggctaa tctgttactt cataYtcaaa aagttagcaa gcaggttcct ggaaacatat 29461 ctgctaacat ttattttctg atgtgtgatt ttccccgggg cagggatggg gatgcaacaa 29521 taacaacaat gagtgacagg cagccctgtg tcattggcct ttgttccccc gaggaaaggt 29581 ctgaaatgaa ctcagcttgc agtgtggcta taggaaatgg catgtctttg tttaggtgaa 29641 aaaagtgggg ctctgttacc taagtgaggg agagacggcc acataggaaa ggctgcaggg 29701 agcttcagtt taggcagtat tcaccatata aacctaacct caaagtgtct tcccatcagg 29761 acaaattctg ttaaagttYa gttttctcaa acacatactt aaaaaaataa ataaaatgac 29821 aacagtaaat ttctgttttc tttgaagcca ggcttaacat gagtggaagc attgtactca 29881 aagaaaaaag tctgttacag cccagtacct cccaggcccc cgcaacaggt ttgacagcag 29941 Mctctataga gtcggcggct tccggttcag tgaacgtgaa tacctcagtc aatatctgct 30001 cctggaccgW gctgctgagc gcagcacaaa gaacacagga attttatcca agaagctggg 30061 gctcagactc tgaatataca ctataattag ggcctgcttt ctctgcaact gaggaccgaa 30121 ttcagtattt cagtcattag aaatRctgca aaacagaaag gagtatacac cctttgagta 30181 caatttatct gttttgtggt tgtttcttaa cRcaaagtaa agtgtgtatc ggcggatctg 30241 gatttatgtg cccgctcatc agaattttaa aagtttagga attgaaactc aaccatcttt 30301 tcctaactat gaggaaacta tgtatgtagg tggcctaaga tagttcttga aaattaggca 30361 ggacttaaac tttttcagtt aatcacgaga aatgataacc aaataataag acatttattt 30421 tttgatttga catgatatgc agtgaaaaat gtgaacaata gcagtagaaa agcataaaag 30481 tagaaaacat gacacatggc attgctcata aaaaagagaa aaataaagca gagaaagaag 30541 tacagagtga agatggagag ggggtgttat tttagaccaa ggttggcaaa cttttatctt 30601 tcaggcattc caggtcatat tctctgtcac agttactcaa ctctgccctt gtattgctaa 30661 agctgccaca gataatacta aataaggaat gtcactgtgt tccaataaaa ctttattgac 30721 aaaaacaggc catgaaccgt atttggccca taggccacag tttgccaatc cctgttttaa 30781 gtagttgttc acagaagacc tctctgagaa gcagaaactg agcagggaaa tgtgtgaagg 30841 gagggagcaa gccataggta tctgaaggaa gaaggctcta cgcagataca cattgtgttc 30901 taacatggct tcYcttctat tgggtttacc gctataatca ctaattatta tctacccatg 30961 ttctaacaga gtcttctcaa atgtgattaa gtattggtca acactccacc cagttgatga
31021 atcacaattt accaaaccac tccagtggct gaatatgtaa actgttctat tgtwtagtgt
31081 catacattct acagcagcaa agactgataa gcatctcatt tttgggttac tcaataggta
31141 cattaggtca aaaggcctga acgtggcttt ttatggctct tcgcacactg ctgtatttcc
31201 accttgggaa tttgcagaag acaatgacag ccagtaacac tgaaaacttc ctatcaggta
31261 ctgttagaaa tagttgtgct catccaggaa gtagaaacta gagcagccaa gtctgtggtc
31321 ttcattttca ctagcaaggc atggaMgtcc ggagcaaggg tgtggagtct gatgcctggg
31381 ttaacWcaag ggcactacct acttgtaagc tgagtaactt ctctatgtca aggtttccat
31441 gtctttaaaa tgagactaat aactccagca tagagttgtt ataaatatta acacacataa
31501 atattatgac tacataaagt ctttaatatt gtgcttagcc tatagtaagt attcaaaaat
31561 gtcagctatg gtcatgatta tatacttttt catctgaatt atctattcat ctcttttttg
31621 cccttatctt tgatattgct atggtcttct taaattgttc atttaagatg gctaatttca
31681 ttcattttat ttccatgtgt cggctctgga aagcagcaat acattaaata gYactcagtc
31741 atataagggg accagtaact gctttaatat caggaaattR gtttgacagt ggagagcatc
31801 tgaYgtggca atagctacta ttaatagaat ctattctatc aaaagtagtc actgagcttt
31861 tattttttga atcctagctt gttggcacga ggtgacacac tcaaacagaa acaaaagaaa
31921 aatgatggtc atagcagtga ccctattatt tttatcatca ttaaaaataa tatttattta
31981 ggagctacca tatgtagagc atgggctcag cctagcaaat gcaaagaaat acacagatcg
32041 tcttcaccct gagagatgta attataattt agttcatgtt ttctttttct tttttggggg
32101 ggcgRtgggg agtgttttag ggtttgctac agtatttgct agactttgca atttgatatg
32161 tcattacact ttacoagtct taataatgtt atggtaaagc aYaaacactg ggtggaggag
32221 gcggggggaa actttccttt gttaatttta agttacacta ttaaaatatt ccaaactagt
32281 tttgacccca aatataatta taaggtttta ctaaatctcc atattttacc ggaaacttcc
32341 ctaagaatgc aacgaatgaa atcactatta catgaacagg cccaatatag tgttttcagt
32401 aagcagctag actgaagagg ctcctttgga ataagtatgt gagctctcag taaagataat
32461 accaggacaa gcaacgtgct aaatttaggg gaggggaact cagtagtagc cctggtcatc
32521 tacttactat ttccttctct aagcttttac cataatgata taccttttct tagagctttt
32581 tctctctggg aagaaatgta tttattcata tgccaaactt gcctctcatc tccctgctct
32641 catccctaac aggcagccat aattacgggg gcgaggcctt acctatttcc agtgcactct
32701 gtctcacagc tgatattaat gatcttcata atactgagtc ctctccctga ggtgaaggga
32761 ggtggccact tttagtggga agaacagctc tccttactta ggtggaatga ttccagagag
32821 tgtcaggcag ccttggaagg agacacattg actcaacaaa gaaaaaaaaa gggctgacgt
32881 acagaacaga acatgagtgc tcattttccc taagacagaa aagctggttt tcgaaagtgg
32941 attagtaaag tggatggtga aatgcaacag cctttatgtg agctggcttg gggtgcagta
33001 ctgttcacat ccacagcaca aagcaagagc agctcacagc tcgactggca agaagattgg
33061 tggggcttaa ttattgcaat ggattagtaa ccaaggaccc tgctttggaa aatgaattgc
33121 agatctcagt atgaaacaga aagacagttc gaaattcaat taatactggt ccataaatta
33181 gaaaaccttg gctcagaaag attgtacata agggctacat ttctttttcc caagaaaaat
33241 ccaaaatgaa tgaaatcaaa ttggaattta aagctacttt ataatttggg tttctacaaa
33301 tacaccatgc ctacttttaa aaaggaagca tgcttaattg agttctaaca ctaaaaaaca
33361 aaaaacacac actcaaaatg caagagagaa agtactttaa ttattactgt ggaaagtagc
33421 cacttgacaa tgcaattgta ttctggggta gtaggctgta atataccaat tgcctacatt
33481 cccaagtgaa attttaaaaa ttagtactta tttttagcct agcaggcatc atttgttccc
33541 atagaataca gtagtgtctc ctcgagttag ggaaggaaca gccctgatgt caacaatgca
33601 aggcaggaaa tacagagacc aagaagtaaa aatatacaca taaaataaga gtatgtgtgt
33661 atagattcag gttctttcat taatgcacaa aataatctca gtaataaagt agactcactc
33721 tgatattttt tctaaaacat tatacttctt gcaatctagc ttaacaagtc tctcaaagga
33781 tttgagagag agggagagag agcaacattc catttgttta ttgggcctaa gacctggtga
33841 attcagtgga gaataacttt ccgaggaaac gggaagattc ctcatatcca gtgtttctta
33901 attttcaaat tacttgaatt aaacaaatac aatgcaggaa ttgtacattt tataacaacc
33961 tttacgtgac aactcagaga ataatactac ttatattcca ggtcctactt agaagttctg
34021 agtctaaatt cacaattttg atttaagaaa caaaatgcca ataaattata aattatttct
34081 agtgatgtat ttgaatccat tcaagtttct atactcattc tacactgcct aaccataaac
34141 tgtggataac accgtaagaa taagcagggc aagctgaagg cattccgttt agcaaactgg
34201 gttctttaac acaaactgac gtatttccgt acatatttat aaggcttagt ggttattatt
34261 agtctgtaat tgcagttgtt ctaacaccta ataagccatg gttggaaaaa caaaggcagg
34321 ttaatttgtg atttcctcta ctcattcaga ggcaaattaa gtgtcaaaca gctaatacga
34381 aacatatttc attttatgag attttaattt aattctgcac catatcgttg atgccttaat
34441 atagggtggt ttatctaaaa agaggagcaa tttataaacc tctttcaata gaatctttaa
34501 tgataggata tgatagcata attatagaag cgcctgccct tcttctaaac agagatctgt
34561 cggcatttcc atcaacacaa atccttgttt ttaggagact cgtaatttag tttttaaagc
34621 tggcaccaga agaacaattg tgtgtacaaa ataatgataa tatctccaaa ttttgtcttc
34681 ttagaattaa aaatattgca aaaacttagt actcttcact tgatctcatt ttctataaga
34741 caattatata gcaaacagtt tggaattcaa gttttggatg aagtgatttt actttcccag
34801 gtaactgatt catcccttag caaatacgtg cttttaagat ttctataacc aagccacatt
34861 tgcctcgatt agcctccact ttcaaggtga tcacaggtgt ctacttatca tacctcagaa 34921 ggtgcaagag caacccaggg tctcataact gagcaagggc agaatagaaa ctcccagtat 34981 ttttcatgcc ccccactgcc tggaatgcca accttcagca tttatttaga gctctgtgct 35041 tttcaRagtg cttacatgaa cattatttca ttgaactctc cctgtgatct gaagaggtgg 35101 gtacagcaga oagcttcatt tccacgttac gaaaccaaca catgcaaagg tcagtggcca 35161 gagtgcacag cacaagtgag tggcaacaca agaacaggaa ttcgagctct ctgactgctc 35221 ctccactgtc tctcagactg ggccaatgtg agtgtccact gagcaccttt tacaaagtgc 35281 ctgaccaagt gctgggcact gcccMtgaaa ctagaaggga gcagtgaccg ttaacaataa 35341 atcaccaagc aagaggaaaa gcatcacaac aaagStacat gcgagtctct gggacggccc 35401 ttaaattagg ccttgaggat gaatataagc aggagttcac catgcagaga gggaaggcca 35461 ctgagaccaa tgcagacaga atgtcagtca caaagacaca ggggcgtgac atgccccagc 35521 ttgctggggc ccacttcata agcatagcgt ccacccaggg ccctgccagg atgcactaaa 35581 accaggcagt cccaggaggc cacaaaccca taagctcacc attctctgtg cccgcagcaa 35641 tgatattccc tctcagaatc ttccaggact tgtcttttga ggctcaggat tatctatcag 35701 ctttacatca tcaatctttt tgataattat acatttaggc tgctagagaa ccatttagtc 35761 agatgctagt aaagtatcca aagcctggct aaaaaagcag gaagaactaa aatccaaaga 35821 aaacccagct caacagtcac acMtgaaatg tgactccccc gactctgcct aSgaggatgg 35881 tccagatgtg acgcatcttg tctcgtgatg ttatcaccac gggccaatgc aggtcagtaa 35941 tgagggccta gaacacacag tRggggctac aggagggctt gctgttatat tccgccatag 36001 aaccaagact tggggttttt attgataaat tcatatttga atttaattac aaggcaaact 36061 ataatgaatg cagaggaaat agttcagcct attatataaa ctcattttaa aaaattgctc 36121 tagaacatca tttctgaaag aatgaagaac tccaaaattc cggagaaaat ctgacttaaa 36181 ttaagtacat cacaaaggct ccagtctggg aggaattagc catccatgtc tccgaggtct 36241 atctggccct Ygcatgctct gaaccatggg gcagatcStc atctgagacc catgtggtca 36301 catctgtatc ataaaacctc tgtataatgg ggtgaattac atgtcatgtt tcaggctgaa 36361 ctaggaattt ccttcccttt •gtatagagaa ttttaaattg gcctcctggt tttattagag 36421 ggtattttta gcaggcttca gaaccctgta aatacttact tgtgttccag tgaaaactca 36481 aaggatcagt cacaagtttc tgcattctct ctctataaat acccacttct cctttaatgc 36541 ttatagaagt ctgcaacaca gatttattac ttacctccag gcaaatctgc tctgaaatta 36601 caattcagta atgggagatg gatccaaaaa actgttggta aaatattata tgtgtacttg 36661 ggcatgtagt taggattgtt aatatcaatt aattctgttc acagaggagg acttgtttta 36721 aaacaacttc atttttcttt ggcataatat gtaattacac agctgtaact aatagccacg 36781 gagatgcaat ctaccRtctg aataaccagc attattcttc aaagtattat ttgtgctttt 36841 ctaactttac tgtctcagaa taaagggtga ttattagata tgaataaatg tatatatcaa 36901 ggccgttagc aagggacagc ccatcccaga gagtggctcc aattattatg gtacatgaca 36961 gtttcctgga cagtactaca aaaaaatact gttttacttt gcaactccac catcctttat 37021 ttcctccaaa ccaaggaaga tggtatttac ttccaagaga tttccttttt ccttttctaa 37081 tgtatgttat cactagcatt ctttcctttt atctttctgt aaaccatgtg ctcgatcaca 37141 tttgccattt tgtttggctg acattcccat caatcaagtg acttctggtg tacttacata 37201 ggggctatga aaatttacca taaaaatttc attacaattc cttgtgatcc ccagaacagg 37261 actttcatgt tacagaaatt tgaacataac aaacttcaaa cctaatgtcg accaaataca 37321 tattctgagt ggtaactttc cattaggctt ttacttttta atttagtatt tacagcccac 37381 agaacacacg tcagcaaaat atataggaaa tacttgtcgg cgacctagtg tgacaggcat 37441 tcctcaggaa gcagcaggga tttgcttcag tgaacccacg aacagatagg cttgctatat 37501 ttcaaaagta aatcttacac ttctatcaca tcttcaaagc ccagtggaca tgccattaac 37561 aaaaagtcct gagatggtaa aagccagcat ctcgttccat ttcattaatg aagaaagtgt 37621 taatacttac atatgcaatg gaggaatcgg agatggatcg taatggtaac ggccctcatg 37681 atgtctggca tcaattggta caggaggatg gaaggcaggg aaaagatgag gagggtctga 37741 aaagaagaga gagcccaatc tatatcaaat gaacactttc aaacagcatt ccgattcaca 37801 aagcaacatt gctatactat tgtatcttca ctacctacac ttaatctaag aatttgtaaa 37861 agaactcttt aaggttagtt atgaagctct gtgaggtact gcagcagtgt gactaagtaa 37921 gtagtgcctg cacctacctg agaaggcaga gcctgggact gactgcaact caaacccaga 37981 cacgaaagca agaaagagag gagcaacaYa ggctccctat cttgaaagac aggctgacca 38041 gaggagaagg gctgaaggga gccacaaagc caaggggaag agattaaaac acacttattt 38101 gaaaaataaa actacattgt caagttaagt tttaRacaaa gacactgtaa tacaaatacc 38161 agtttcattt ctcccatgct caccatactt ttaagagtga aaggcttgct agaatctgtg 38221 gctgatatac acatgcatgc atgtgcttct tttttttttt tttaacttaa atacatattc 38281 ccctccttct tggccgggct aggcctccat agtccccacW caaaggcagc aaatctatgc 38341 cctgtccagg gaaacactgt gcagccctcc aggaagagat aagctgaccc actcagactc 38401 acagacatct agagccaaac tcagggaatt cctcccaagg tagtgttaac attcaccttg 38461 gacccaggcc ctctcctgct tccacctttc cccagtccaa cgctccgggt tcctgagctg 38521 ctgggtgtgt gctcttcctt aaaaatagct ctcccacccc cacttactcc tagtaactca 38581 cacccattgt cactgaaaat aaacctttat taaaaaaaaa aaagtgattc actgtaaaaa 38641 ctgaagagag ttttaacaga ttttatactt agaagcatga ctgatgagtc aaggttttcc 38701 caagtttcaa gaagcaaaat gaaggggtcc aaaacttgga ggtttgactc catgtctatg 38761 agactaaata cgacagcaaa atgtgccagg acatgttctt caaatgtgac gggtccacac 38821 tggtcatgct gggagcctgc ctagagccaa cactgctgag gattggtgtg tacacccaca 38881 agcctcagaa tggcttccag ttatttcacc ctagtgctga gggtagcagt ctctaaaggc 38941 aattccaatg gggccttctc tcagcggaca ggttctgatc gaccacctaa gaatgcacag 39001 agcgtccccc cgctgaagtc ggctgcatcc cccgaggtcg gcaacaccgc catcccaact 39061 gggtggaaat ggatgattat tagatgtgtg taatgttgaa ttcaggtggt tttgattttc 39121 ttccctaccc atttctctat tttccaaatt ttcttcaaca agcaactagt agaatataag 39181 cacaacaaac atgtttttaa agttatagta cattgcttcc cttgttttga ttttgtccct 39241 gctgccttca agaatataag actctgttta tgaagaaaaa aatccagtaa gagtaaaaaa 39301 aacgatgagc atagcattca tagaagctct gtggtctacc agggaaggac ccgaggctgt 39361 aattctacag tttaccacca gatctagaat ctagaaaact tcccagagga ctattttact 39421 gaaatgtcaa tgactcaccg tactttgtga ctgtcataag attgagtagg gagggagtgg 39481 ggggcatgga aagaggaagg aatcagtttg atcttgtatc actgtcactg tagtatcgtg 39541 atatacaaca atgctgaaaa ttcaacaggt gagggatata ctaatgacct gatcatttat 39601 cacagttttt tttttttttt ttttttgaga cggagtctca ctcagtcacc cagactggag 39661 tgcagtggct cgatctcggc tcactgtgag ctccacctcc cgggttcacg ccattctcct 39721 gcctcagcct cccaagtagc tgggactaca ggcgcccgcc accacgcctg gctaattttt 39781 tgtattttta gtagacacag gctttcaccg tgttagccag gatggtcttg atctcctgac 39841 ctcgtgatct gcccgcctcg gcctcccaaa gYgctgggat tacaggcgtc agccacYgcg 39901 cccggcctca tttatcacac tttacacatt ttgtaaagtc catataaaaa ttgattatat 39961 tttaagttta ccacttagta cttaatatga aagggtgtag gtattattat ttgtaatgta 40021 aatattgaaa gttgatttgg taagtttgaa agtttaagtt gaatgtttat tcagcaatgt 40081 tggcaccttc attgctgcac atagYtcagc catcttgaca gttaaaagcc accctctgta 40141 gagccaaaca ttctctctca Wttaaccatt ttcaaaggaa attatgcacc cttcttaaaa 40201 caacaaaaaa tYaagtgggg tcatcttttg cactcgtttg aaagcctgac atatactagt 40261 ttatgtttac tgattttaaa ctttaaaaca aacccaaatc aatcagattc aaatccgggt 40321 gagggtggat ggccatgaaa tatgggggtg gctcttcttt ctcttccaca cctttcagct 40381 atcactgcca atgcaaacac gcatgccccc tgcagacttc ttacttaaca cacRtcatct 40441 gctcttggca atcacagaaa taaacttact agaacagggt gtgaattcag cattcataaa 40501 gtcgtaccca tctcctcagg gcatcgcaca tgcaccagtt tatgcggctg ttttgaggga 40561 tactgacaac tatgtcagag ttggaaggta ttttcacttc tttatttcat ggcttttggc 40621 tgtgttctgc atgatgaaat tctctccacc aggtaagtgg gtgtcaactg tatctggttt 40681 tctctatgtg ggtgcaacta tgaattatga Maaagtacaa aaccacgttt ttcactcaaa 40741 atgtgtgaat ttattccaca tttaatatat gcctcatata tccccgaagt aacagtcaca 40801 atcttttata tgagtgttta taatgaagtt gcagtgatta cgtgtatgga gtgaagacca 40861 accagttaat cccataaaat caaaagcgtg tacccaacaa gtataaaatt atcttcctca 40921 aggaaaacct acaatgctat caaggacatt taaaagacac tgccatcttc tcattttcaa 40981 gaaattattc tttcagggtt taaattttct ctctctcacc ctctctccct ctttcagaat 41041 aaaggataaa atgacctagc actgcaacaa taaatttgag aggaaaatat aaaacacaat 41101 tactgggaca ctaagtagtc atttctcact gttgagtcca cagtattcta tgaatttaaa 41161 agttaattct gactgaaaat atgtgatgca tctcctaaca ctgcaccaaa ttcttaatgc 41221 ttatttatgt cccaagaaaa tttatgaatt ctgcattcga gaattatgtt taaataaaga 41281 acagaaaatt aatggatatg atctgatttg accaaagacM tctgattttg gtttaagtgt 41341 ctcactgttt ctacgtttcc cctcttgatt aaatttaaaa taacttctcc tggattgcag 41401 aagtctgtgt ggacaattat accttgtgtt ggttattagt agaaatcaca cctcttaagc 41461 cactttctgg gaaaatacaa tggaacWccc ctcttccaat tcaagacgtg ctYgactata 41521 gcttaacagg ataaatgaag aatgaaaagc agtatgggca tgtcctgtaa catacgtggc 41581 atctcatggc attaatccta acacatttct tgaaaaatgc tcctgaatcc tcaaacgttg 41641 agtacttgcc ttgctggtgt cttggtttgt cgtttctatg tggctcagcg gcatattcca 41701 tcatcggccc aaaaagctgt ccaagaaaac ccacatgacg agaacaggcc cttgtcgcca 41761 gagccgccat aattacccag ctcgcagttt attatttaca catgagcagt gcagctgtga 41821 cgcatcataa ctcaagcgta tatgtaataa gttactgcac cgcagccatc tcctgccctg 41881 ccccttgaca aaacaccaca tgatgacttc tccaaagtca ggcgaaaact gctgaagcag 41941 aaggaagtgc cacgaaacct ggctacctcc tatttatcat acaatttcct gactgatggc 42001 tccacaaacc agctgctaag tgctgtgtac tctactgtca gcaaactaac gggctaaatg 42061 gaagacaggg agaataatgt gcagaactgg tggaatcaat tttactgagg gaattaaaat 42121 tctaccaggt gggggcgggt ctgtggagca gtggtaagca gtcaactgta catgcttatg 42181 gttgcaaaga tgatttatta cagacctgcg agaggtttga ctattgtacc cgagtcctct 42241 tacactaaac agagatttga agagtgtgca ctctcccagg cttgagtgga atggtcttta 42301 aaccctataa atatgtaact ggcttgacac atgtttaaac tgaaatgcat agcaaaaata 42361 tttgtgctgg gtagagttgt cagtattcca tttcacactg gctgtcatca cgttgagcat 42421 taagggtctc attaggaacg agaggcattg atttttaggt ctcttcacaa cagcctgggt 42481 tgatcataaa acctgtagcc agagaataac cgagaaagac acttggagat ttatctctag 42541 ttgcatcctt ctcccaagaa atagttatag tatcctcagg ctataagatc tcttggatgc 42601 catcctggga aactgtcttt taaggtttga aatttacata caaaaattgc agattaaaaa 42661 agagagatca aagtctctca gttagtgccc tgaaggagta attcaccaaa tgatacttga 42721 atggtctgaa agtgcccggg aaacagtgca agagtaaact gctaagtgca gaagtcctca 42781 tgggtgattt aaatactgaa gtcgtctgta ccctggcctt ggaatttact caRtcagctt 42841 ggaatcatct caatcagcta gggaYgattt tctgtcatta gagttagtcc agcctgtagg 42901 aatggccagt cccctcaaac tcattcaatc atctgccatc agcagaaata tcattaaaca 42961 gcatccgcta tgctacaacc aaaatcacca tccagcttct ataaaatgaa aggtctcaag 43021 caggagaggg agagaagagg agagacagaa tgagagatcc gactgccaag gaactcactc 43081 tagtgagata gtagagaggc agcccaggcc tcatcactgt ctcttcccac catggagctt 43141 ctccagtcat catcctcgtg cctgcaagtc gtttttccag ggctcagtgc actgtgggag 43201 gccatttgga ctgactccaa gatgcagact gacttactaa ctcagacaac cttgcaattc 43261 ctgcctatgg atgacacggc accagaaatg agctgatccg ccctctgagg acaccccagg 43321 taacctgaca aggagagtct agagcaacgt gttttgagca agRcagtctg tggcccacca 43381 tgatctaccg tgagcggaat ctaagaagtg ctacccccac agggccccgc tgaggacttg 43441 ctgggcagaa tccccacagc atcacctcat ggagcactag gaatcttttt tctggtctca 43501 caagcatatt atgtggggtt agaaactcct ccggaatcat cagaatgaga catcgtattt 43561 atatgatctt ctatagggta atgcaagttt aaagaagctg tggttctagc atactctaga 43621 aatcctgtaa aaatgccctg aaactttttt tttaaattat ctattatttt gtcactttac 43681 acaatggcag gagccttaac ttgaagagta tatgctatag gaggaaactg caaactatca 43741 catgtgggcc ggtttttgta aataaagtta tgttttaatt ttttaatttt ttataggtac 43801 atagtagatg tacatattta tgggtcacat gagatatttt gacacaggca tgcaacacat 43861 aacaagcata tcagggcaaa tggggtatcc atcacctcaa gcatttatta tgYgtgctat 43921 aaacaatcca attRtactct cttcattatt ttaaaatgta caattacatt gcttttgact 43981 atagtcacca tgctgtgcta acaaacattg ggtctcattc aatctttcta tttttttgta 44041 cccattaacc attcccactc cctgctcaac ccccactatc cttcctagcc tctggtaacc 44101 atccatctat tctctatccc tctgagttca atcattttaa tgtttaggtc ccacaaataa 44161 gtgagatcaa gttctttctg cacctggctt atttcactta acatcatgac ctacagttcc 44221 atccatgttg ttgcaaatga caggatttca ttcttcttta catctgaata gtactccact 44281 gtgtatatgt accacatttt ctttacccat tcatctgttg atggacactt aggttgcttt 44341 gtaaataaag ttttattgga atactgccat acttatttgt ttacatattg cctatggctg 44401 ctttcccacg ccaacaacag agctgaccag ctgagacagg aaacMcatgg cctaacacat 44461 ttgttatctt atcctttatg gaaaaaggtt gacaaaccct attccagtgt accatgataa 44521 agtaatttgc agaaattatt acatttcaga aaaaaaaata tcaatttttt atttattgtg 44581 ttgtctaagc gataacacta agtacatcaa gtattttcaa tgcactttct tcatatgcta 44641 acttgtgtga ctgacttaat atagtaaaag tgttcatatt aatcattaat caatagacat 44701 ctcatctaat tttagcaaaa cccgtaacaa gtagacaaat ttcaagataa tggatgaaat 44761 taaccataaa tctagcaata atagcagtag ctaagcattt gaaaattgaa gttaccttat 44821 aaattaagta aaacaaatac aaatttacat aaaatgctaa ttaacaataa aaagtaagct 44881 atttctaaaa cccaataagc ttttacttct ggcaactacc tgacagtctt gactcctggt 44941 ctataaactt ccacggacat tctccactac tcaaataaca agctttttat catttctttt 45001 gtgtaagcat gcagactatt aaattaatta gtattttaga cctaaatgct acccatcact 45061 acactctggc cacacccaca attcatcctt ttttttaaac agtcagatag atcccttctg 45121 tgctcctcaa caaacatggt catgtgtttc ccacaagaaa atgccaaatg ttcctctgca 45181 agtgaactga caaaagaaac ctctSccttt actgcttcag gtatgtcctc agtcRtgaac 45241 caacaactga tctcgcccct ttctggaaac ttctaacttc ttctgtccgt aagtctgcat 45301 gtttctccct tcaagatcac atccagtgga tgcccagtgg tggaaagaaa accacatcta 45361 cgtactggct gaccagccat atccttccaa acagagactc tggaatgtgt cttgcctgtg 45421 attccacata tatgcatcca acatttttgt tgttttcagc tttcctgcca ggataagaga 45481 gaagaaataa ccagagggaa aggcagcaaa gagaaataag gaaaggttta catccattct 45541 agctgaactg cagaactctc tcagggaaat cacatgtaaa catataagga atcacttaaa 45601 agtgacaaat ctgtttactt tgaaatatgc taatggtgta ttttcagtga caataaataa 45661 gcttggaaat taaaaatgga aattagttac cagtgacaaa tttttaaatc aatgactgta 45721 tttttgtatc acaaaattca aaagtcacag ttatagctct aaaaatatgc atttggattc 45781 tttttttttt tttttttttt ttttttttta gattgagtct cactctattg cccaggctgg 45841 actgcagtgg cacaatctca gctcactgca acctccacct cccgggttca agcgattctc 45901 gtgcctcagc ctcccgagta gctgggatta caggtgccca ccaccacgMc cggctaattt 45961 ttgtattttt agtagagaca aggattcacc atgtgagcca ggctggtctc gaatggttat 46021 gaaatgaaag cagaagagga aatgtttcca taaaaactgt ttggttatct cagatctaac 46081 tacggagacc ctggagtcaa taggatcaga agtttaaact aagcctggtt tagttaggaa 46141 accaacaact gatccacaaa ggggaggttt ggaagttgta ttctattcaa aattaacctg 46201 ctgcaaaaag agctttcagt gttgcaaaac tagcagaagc aggtagggaa atgtgaaagc 46261 aaaataagat agtatatttc ataatagtat atatcttctt aaggaatcct cttgacataa 46321 aataagcatt cccaaaacgt tattattatt ctcctaagtc ttgtttcttc ttaagttcta 46381 ttatcaagac aatcctgaga actaagaagg gaaaatttta ttatccactc aaaaaagcta 46441 aatgatctac ccagtgttgc ataaaactat ggccttatga tgcaagtttg gtgctctttc 46501 tattccaata ccagagcaca caggacttgg cacttcaaac Rtgatccctg ccagggcagg 46561 gaaacccagg taccttcatg aaMtgtcaat cctgctttgg aggcggcaac cctgaggagc 46621 tctcaggcag cacaaatgca agcctgccag agattgtcgg ggctcaggct cagcatatca 46681 agaggtttac ccttgtcttc acagttgcac aagagagaat gctgccatca atcataaaga 46741 atcacttcca gaaatatttg gaagtctctg gaagaccagc agtatttcaa gagtgtgctg 46801 gttggcaact ttgtagcaat taagaaatga atgaggcaca ttcattttgc tcaaaaccag
46861 gcaagaagct ctgaaggatt attgactctg cttcttttat ctggaccatc cactccatct
46921 gcagttcctg tcaatcaaca actaaaggtc ttccaaggtt tgccccataa atagtaatta
46981 ataaaaaata ataaaacaat cttgatgagc acaagtgcaa gtcactgaat actttaatgc
47041 aggtcaagca atgtgcaatg ccttttagat tcacctcatt tatttcttcc tggggcctga
47101 ccctgagaag caggtactat tcatacgccc attcttatgg atggggaaac Ygaccttctc
47161 cagtgttaca gggggagaca gtagtagagc tgtgtgtctt tcttacttca gaaccagggc
47221 tcttagacat tccacttcct gacacaacaa tgtcagagtt aaggcttttt atcatagctt
47281 atctaaacta tactccccac accagcatcc ctaaaaccaa gtctgaacca tatccctccc
47341 ttctcccaaa atgtatagta gcccaagata gcctccccac tccagcccaa gctcttcacc
47401 atgagtttga ggccccagcc accaagctct gtccaggaca cctccccatc cgagagtcag
47461 tgccccctca ggcaccatgc acttcatggc ttatggtgtc ccctcagttg aggacaaatt
47521 ccctcttacc caagtcttac cccctcttca agactcaata caaacaccac cccctccatc
47581 aggcttccca gggggctcca tgggatcgag tgctccttcc tcccttctcc ataattcagg
47641 ttggccagaa ccagtaggga cgacccgagg aattctactt ggacccgcat catgtcctcc
47701 ccactgcctt gccctctgac ggccctgtgc tcatcctcag tgcgcttcct cagacaagca
47761 cacatttact gaatacagag cttataccaa cacagccatg ggggtgagca caccacactg
47821 cctttcctgc tgggcagtat ggggatgagt ccctgccagg gtgcacacac ccacaggtca
47881 gaaYtgtctt ctttccctcc caggaggtct ggggtgtgag ggcagaagag gcccacttat
47941 ccgtgtatac ccagcaggaa ctcaataaat gtatgttcta ggaaaggtga aaagggggag
48001 gaaagacatg aaaggaaact gaggccacgc aatgacagtg gtggaccagg ctagccatcc
48061 ctcctggaaa ctgcaggaca cttccatcta cctaaggggc ctccaaoaac aggcaaaatg
48121 aggggaaccc acaggtatgg gctgtctcct ctttggaatc ctccctgtgc agttttctgc
48181 atgcttccca tgttcagagt gagcccaggg ccaaggccca gcagatgcat gcacaccctg
48241 gaccaaatcc tagcactctg aaaggcagct tccagcYtga ccaggctgcc tgccttctgc
48301 tgggcagtga gtaacttcag tgagtaaact caccacccta ccccatgcca ccacctctaa
48361 gacagggcag acactgatgg gcgctcagca atcacaatcc tggttgggac cttttgactg
48421 agaatgccct ttcccatcag agaaacactg atggctccag tgaattcagg atacttgatc
48481 aggctgctaa cacccatatg gaaaaagctc ttagacacaa aaggttggag gtcaaaagtt
48541 taaaagctca tgacagaaat gacaggggcc cctttgcccc caccaccacc tacttttttt
48601 ttttttttca tcaataacat caaaggctct ggcatgtaot taatgctatt attgttacgc
48661 tttggaatcc attgaaattc cagcatgttt actgcacact gggagcatta aaaggctcca
48721 ctttcttgtg tcccttaaac tctgaaccct cactgtctaa gaagcatcat tgtttgcaga
48781 aacacataaa gctttaaagc aaagagtttg ttttaagggt ttctttcacc atgactgtaa
48841 aacaataaag cacacgacat ggcttccttg aaaggatgag caaggaaatg atagaaaagg
48901 gagagaatcc cccgctgctt agaaagaaaa aaatccactt cacatttctt ggctcatggg
48961 agtacaagta gaaaacattc cccatttttc ttctatttct cctttctctg caaccctatg
49021 ttagccacaa gctgcacatt ttacacacac ctcaaagaca ttggctatgt gagcatctat
49081 caaatggtat catctgaagg agtccgaaat acctttcttc tttagaatgc cttctcaagt
49141 ccaccgtcca ctttgcagct acctcatact cattSctgga tgtgcagata aaaagcccca
49201 cagctgcctg caggctggtt cagaactact ccaagctcca ctctctgaag ttcccactag
49261 tggcttcctc tcttgttggc tacccttocc ttcctcgcaa tctcatctgg cttcctattt
49321 tgattcttag cttaattcca gttggaatgc tgaatgaatt tcagactatc tctgaccgtc
49381 caccccttag agcacctgct cctatggtaa tgccttggat gtctaaccta tgctataggc
49441 aagaggcatc ctgcctgggc ctgcaagccc accatgccca taaaaggcag aggccactca
49501 ttgcccactc ttcccctcca tgattcctgc agtccagaca agctaatgtg ctcagacaca
49561 tcccagagga attcagtttg ccagcgttct gttttaggac tggggaggat gataggcttg
49621 ggggtgtcaa caaacctatt cagYtgggct ctgatgggga ctctcctctc tggggaggca
49681 gtggtcctga ggctcctttc actgagccta ggccactggc tgtctgctgc ctgtgcttcc
49741 ctgaggcctc aagctctctt gcgcacttcc gcttctgcag cctcagtggt gatctcactg
49801 gcccttcatc tggactcagc agctgcttac cttgtctaac aagactcagg ctctaatgtg
49861 atctccatct gagtcctctc ctccctctct acaaagtaac ccctggcttc tagaccagtc
49921 ttcataaact atacttggta ggtatctgat gtcaatgatg tccatgtgct ccaagtgccc
49981 ctaaggacag agaacatggt ctgcttctgg agtctgcatc tataccttgt acgcctaggt
50041 ccacacctac agccctaggg actatagcct caacacatgg atgcgtaata gtggacacac
50101 tcacacaaag atgaccccaa tctgcacttc ctcaatgagt ttccactaat cagagggtgt
50161 ggcctacact catttYgaga gtgcaagact gcttctcatg cttctttgct gtttccaact
50221 gttactaatg acttgaagca tgataatacg ggatttaaac aaaacatttg aaacaaagca
50281 tggatttaat aactgacact aggcaacaga ccaaattgat ggcaacctac tggtcatctt
50341 gacaccaata tatacttgta cagaatttat caccctcaat atgaatcctt agaaaagcca
50401 tcctttctac tgagttttac cttctccaaa atcgggaaaa ggccaaatac tcagcacact
50461 Saatgatgca tgctgcaacc cttggctggg tacacccttc ccatgggaat ttatctgctc
50521 tccattttgc ccaaggcagc tgaaaaggct cccccattta aagacgtatt ttctagattt
50581 ctataaaaac aaattacaat aatcactaca gccataatat aaattttctt tcatccgtaa
50641 aataaggcta gtaagactta actctgttga ctccacaaag ttgtacaagg ccccaggtga
50701 caYaagtgaa ggtggttcat aaattctgaa taacagtcat gcatctctta acaatgggat 50761 gcattctaag aaatatgtct ttagatgatt gtgtcattgt gtgaacatca tggagtgcac 50821 ttacacacac ctagatggtg gagcctacta cacgcccatg ctgtatggta tagcctatta 50881 ctcgtaggct ataacggtac ggtagcatgt ccccatactg aatcctgtag gcaaccgtaa 50941 cacaatgata aatattggtg tatctaagca tatctaaaca tagaaaaggt acagcaaaaa 51001 tacagtatta aaatattttt aaaatgctac acctatatag agcacttacc attaatggag 51061 cttgtaggac tggaaattgc tctgggtgag tcagtgagtg agtggtgaat gaatgtgaag 51121 acctaggaca tccctgtaca gtactgttga ctttataaac actgtacact taggctgcac 51181 taaatttatg aaaagtaaag tgactgtact atgacgttat gatagccaca atgtcactag 51241 gtgataggaa ttcttcagct ccattataat cttatgggac cactgtctta catgtggtca 51301 gtccttgacc tgaatatcct taggcagcac atgactgtac ccatgtttaa gaagtcaccg 51361 ttccttctaa gtccttcttc ctcaacataa acacccaagt taccatgaga gtcaacaact 51421 ttatgtgggt gtcttcagct ttgcaagtaa tctgaaggtt caggtcatgc ttctctcttg 51481 ccctcaagca ttctctgatc ataagaccca ggaagagtcc tccatatctc tgtccctaag 51541 taaggtctgt ggcaaggacc tcactctgtc tcctgaactt cacactagac agacactgtc 51601 ctcagcttaa ccttcaggga aaagaaaaaa agttacagct acaaatacgc tcaaaaaaga 51661 actatcattt actgagcact cacaggtact aaggactgcc ctaaacaYtc ttcatatatt 51721 ttcttgctta taccacatga caatctaagg aagtaggtat tcatatgcta ttttgcaaat 51781 gagaaaatag ggatagagaa ggtaagaagt taaagtcgct cagttaggaa ctgacaaagc 51841 ttggcctcaa gtccagatgt gcctgactac agtgctctgt aagctacagc agggccaact 51301 acccaggcat ggggcctgtg cagcccctgg gcctgtggtc agaagggccc gagcatggtt 51961 taatgttacg cgcagtagcc Rtcttgaaat tcttagtaat ttttcaacaa gggctcccca 52021 ttttcttttt Rcactgggcc tcacaaacta tggagctggt cctgagtaca cctgtatttc 52081 ccacaaaagt actcccactt ccaaccttct ctgcttcacc cttgccaacR acaaaagcta 52141 aaagctgcta acagacattg tcatgggtac actgggtgat tgcacacatc ctgccttcac 52201 tgcccaggta caggtcccct acctcaccca ggccctgcct caggcttaca ctttggtccc 52261 ctKgccccag tcgctcagac acttctccca ggcaccaggg acccagccta gcaggcagca 52321 ggcccctaac caggccagat ctggcagaac acgggccaga, catcaacccc acgacgatgc 52381 tctcttgctg ccactggggc ctgcgcacac tgtgctaaca cagtcgccat ccaccggcct 52441 gctgtcctga gggctaattg ggcttgttta gaccgaacac agcagaagtc cctctacaca 52501 tatataaatc acaattacta actgcaagaa tccagccgct ctcttctccc ctcgtggctg 52561 ctacagagga attcccaggc cMgccaggac tagcacaaaa gccccagact gggctgcagc 52621 aatatccgct cgctcggctc agtttattat ttaaacagtt ccttaacgat tcttcccgga 52681 ggtgagtgag, gccaaactgt tttccttccc tccacaaagc agatatgcat ggcttatttg 52741 aatgtcttca acgtttactt tagtccccag ttaaatacct tctaccgcag agtcaaacaa 52801 acagttcact tcattagaaa ggcaaacaat ttagcaagag aataccacca gcacttgagg 52861 ggaaccaagc tgggcctcga aaccctctgc ctgggaagca gggccagtgg cggcccaggc 52921 aacagtgcgt ggagctcact tcaaaagcct ttgcacaaat acttcacaca ttcactggta 52981 attaacagat aaccccgaag agattatctg ccctgcggcc aaggaccctg gaggattatc 53041 cacaccaccc gtgcaggaga aggcagagtc caagagcaca cctttttctc tgcaaatgca 53101 agtggataaa gtcatctgag aaaacaccaa agcgagcagc tacacctccc ctaccccggt 53161 gaaaataata acgaagcaac acctcattaa gataattatt aatttaagca acagccggaa 53221 gttgtgcctg agaaaaacac acaggctgga gcagcaagag gacacaggga tggaaagttc 53281 aagacaagaa ccagggcagg agcgcaaagc ccgtggtgtg ctgtcaggat tccagctcag 53341 aagtggagac aacgctgttc ccaacaaaga cttggggcac actgaaaaca ctgtgggggc 53401 tgaccatgag caggttaaag tctgggcact tctttgcgag agaccctgag gcagggtcag 53461 ctgcccattg aggtttgaag ccatctgtcg cacagcaggc aagccccgac tctcagtcca 53521 gtgggcaccg cactgccccg gcttttatct cagaaccaca ggaggaacgc accagagtca 53581 gctcttcagt tgaccaacag atgacaggtt atacctatcc atccagggca caggatttgg 53641 ggctcatctt ttattttatt tcattttatt tatttattta tttatttatt tatgtattta 53701 tgtatttata gagacggagt cttgctctgt tgcccaggct ggagtgcagt ggtgcgatct 53761 cagctcactg caacctccgt ctctggagtt caagcaattc tcctgcctca gcctcctgag 53821 taactgggat tacagacacc cgccaccacg cctggctaat ttttgcattt ttaccagaga 53881 cgaggtttca ccatgttgac caggctggtt tcaaacttct gacctcaggt gatccacctg 53941 cctcggcctc ccagagtgct gggattacag gcatgagcca ccgcgcctgg ccaggatctt 54001 gtttttaaaa acagaagggc gcccagcatg ggcaaatcac ctgaggtcag gagttcgaga 54061 ccagcctgtc caacatggag aaaccccgtc tctactaaaa atacaaaatt agccgggtgt 54121 ggtggcacat gcctgtaatc ccagctagtc gggaggctga ggcaggagaa tcacttgaac 54181 ccgggaggcg gcggttgcag tgagctgaga tcgccctatt. gcactccagc ctgggcaaca 54241 acagcaaaac aatgtctcaa aaaaaaaaaa aaaaaaggtt cccttctctt ttctacataa 54301 gaaacacata tactcaccac cactgtcccc catatatacg gttaccacaa tcgtagacag 54361 taacatcaaa taaaaatggg gtgggtggtg cagctgttcc tattatactt aaatttgggt 54421 tttcacacat gctgtggttg ttctggacag ggtcagatta agcaatttca gtgcttatgg 54481 ctcttaagaa tttggtaaac ctcaaatgYc aactgtaata aatcatacaa gtctctatta 54541 taactataaa tcactataaa tcactcatga aatttaatac tgtcatgaag agtaattaaa 54601 tgctaatgta acaaatttac aaaaggaaaa acaaacaYat taatggggaa tcttaagagc 54661 tagagaacac tcctgtatgg gagctttcag aaaaatcttt gtccccaaag aataaacact 54721 gtttctttat ccaaagccag aggtcctcag gaagtgaaaa tcaggaaagg gccacactcc
54781 ccaaataaac tcaggactga gtgctaaagt ccaactccct tcttcaatgc agaaaaaaga
54841 taccaaaaat tgactttcat tctgcattat cctactaagg aaacagagag gaagggcgca
54901 aaagtcaagc aggagatgag gagaggccag tgcccaagaa gggtcttggg gtttaaagga
54961 agtttcagga aggagtcaca gtatttccct caaggtcact atctcccttg cactgcccgc
55021 ccttcaaatc attgctgggt tgatactcag ctgttcttag ctcttcaacg atgctgactc
55081 ttaaagttta gacaggacaa ttagaaccac gaattctgtt ctgtgctgct tcagtcctaa
55141 cacctcagga ttcagtaagt agttgctgaa tacaggaaca agtcaaaacc aattσactag
55201 acagggtgtg gtggctcaca cctgtaatcc cagcactttg ggaggctgag gcaggtggat
55261 cacttgaggt ccggagttca agaccagcct ggacaacatg gtaaaacccc gtctctacta
55321 aaaatacaaa aaattagggc atggtggtgg gcgcctgtaa tcccagcact ttgggaggct
55381 gaggcaggtg gatcacttga ggtcaggagt tcgagaccag cctggccaac atgtcgaaac
55441 cccatctcta ctaaaaatac aaaaaaatag ggcatggtgg tgggcacctg taatcccagc
55501 tacccgagag gctgaggcag gagaattgct tgaatctggg aggcagaggt tgcagtgagc
55561 caagattaag ccactgcact ccagcctggg tgacaaagtg agactctgtg tcaaaaaaaa
55621 aaaattaatt aaaaaaaaag acaattcact ctgcagatct aagcagcact gccaggtacc
55681 aggccccacg gattcccaaa agcctaataa gccagttgct ccaaggagcc ccagagggga
55741 aggaagcacg tggactgagg agtcacaaag ccacccacaa gcacaacagc tcaaaagcat
55801 cccaggtcca aagacogcac atgagggagc agtgggcaga ggactaggta ggggaaggat
55861 gagggccaga ggaacctcct ctccttgagg aaaagccttg ctcagaaagg tgaccaggag
55921 tgtggagaaa agacaccaca gacaagcaca gcctgctcgg gggcaagggc gtgcttggtg
55981 actcctaagc agctcagtgt tgctttccag catcggtcct ccaacttgac cttgcctcca
56041 agttcctgca gagcttgtta caatgaagac tgctaggctc caccccagca gtttctgact
56101 cagtaggcct ggggtggagc ctgagaattt gcattcctag taagctccca gatgaggctg
56161 ctgctgctgg agaccctccg ggaaccacgg aagcgtgcaa gaagatgagg ccaggcctgt
56221 tcagggctcc tacaccatgc aagagagcac ggaaggctca gcaggagact ggaatgatca
56281 aacacatctt ttggaagtat tactttggtg gtaacatgga gaatgggttg gagtggaatg
56341 aaatgaaggt ggagaacaag agatgtaaag actcaaaaaa ccaaggcagg gcagtggaaK
56401 ggaaatgaat gggatggagg aggggctctg ggagaagcag aagtaaagga cacaggagat
56461 gggtgagaag agaaagatgg aaggtgggct gactctaagg ttctggccca ggtgagtgtt
56521 aatgatggtc ctgggaccaa ggcagcaaca gagaaggaag caggagagga cacaagagga
56581 gggaggagaa agcatggatt cagcctgaga caggtggggt ttgaggtgac caccacagcc
56641 aggctcctgg tagatgtctg gagggaggtg gatctgggat ttgagagcca cgtctagcct
56701 ggagatggag gcgggagagg cgatcacagg agtggatggt gacatcagag ttagtgatat
56761 ctccagggaa ggtaSaggaa gaggaaacct ggagggaggc tggcagttaa gggatgaggg
56821 gaggaagcag agttgtgcaa ggaggaaata ggagagacac aggacaacca ccagtcaggg
56881 gcctccaagg ggccagaggg aggaggcagt ttcaggcaag atgcacagct tccggagcca
56941 gggtgtgtgc actgcaggga gctgacgggt ggagaagcca gggtgaacac tgtggtgcac
57001 tccgggatgt tcggctgcaa atagagaaga cagggcagca gctagagaag acccaaggcc
57061 agaagtgctg tgtggatgtt ttgactctgt ttgtttatgg gtggaaggca ctggagccac
57121 cgaagtggag gaagaatgaa gtgagaaagg agagtaccga tggagtcagg gtccctagaa
57181 tccagaatag gacgcaggtt agctcaaact cgcttcccca gaaataaagg agagggatgg
57241 gagagtacag gaacgggctg gctttaaaga gtgtccagtg tcaagtaaag cacctgatct
57301 ggtcccaccc ctaaacagca ggctcacggg agaaacttca aacttggtag cattgctgag
57361 tcagaggtca tggtttaaaa ggggagcagg gtacagggca ttcctcttgg gagtttcagc
57421 ctgaaggacg cattcaaagY ggtcacacct ttgctgccca ctggcaagga gaactgctaa
57481 gatcctccaa gagcgggtgt tcagggtccc ttcagactcc tgaccctcca ctttgcctgg
57541 agcagccaca tccctctctg cctggagagg gggaaggaag agattccaga agccaggtca
57601 gagcatggag agtaagcagc aagggcccag agaactctgc catggaaatc agccatgaaa
57661 ccagactcca gccacagagc tccaagggcc tggtgccatc aaagataggt tatctcaaaa
57721 gactgttgcc taaaagttgg atgtgattgt gagggttggg gaaacacttg gaggctgtgc
57781 tgcagtggcc aggccaagcc aggttttaca agttcacagc agctgcggtt gctctgcggc
57841 cagcctaagg gatgtttaat aaaaagctgt gctcaatttc acccccttca catccctgaa
57901 ttcagcctct ttatttatag ccccagacta ggcatgcagt gggaaatcag agaggccagg
57961 aaaaggagag ggaaaggaga cagacagaga tagaaggagt taacaggcaa cagcttttaa
58021 agggtaaagc gtttcagaaa cagagaattc actggcactc cctgctgctt ttattgcttt
58081 cgttcaacag ctggagaaaa tgtttgatca aaatatttgt attattgtgt ttaagtgaaa
58141 tgacatatga cagctcatta tcccaaactc agaattccca cagcatctgt cacagccacc
58201 ctattagaga cagtcgctcc ccctggaaga tggaggggca gggggcggga gatgtgagac
58261 aggaagcagc aggcatcaga ccccagacgg tctcagcctc acaggccctg gcccaggctc
58321 tggttaagca cgaggggaga tgctggaggg agacgcaggt gatgcctggg gcctaccccg
58381 ggagcccccc ttcccctgga caccagcagc ctgccaggag cacgggaccc agcaggggct
58441 ctcgtgtcca tggaaatggc caggatcccc tctcttagac attacttggc aaacagcatt
58501 tactgatcta aaatttaaca aaatgtctct ctcaagcctt tgtcctttga aagaagaaaa
58561 aataactttt taaaaattat catgccattt tctctgtcct gaagtgagta aacgtgtaat
58621 tctgcagaac ttggctttat gagcctacgt tcctctccat gacaaccttc aagggcctca 58681 gttctgctca ggccatgtcc ttctacgaca cccctagtgg acagatgtca gggtatatgt
58741 tctataccag acataccagg gagggaaaat catctaccag ctgcttcctg tgtgcctagc
58801 ccatttctga gaacttactg gccttattca aatcccatga caatcccagg aggcatatga
58861 tattatctcc tctttgcgga tcagattatg ctcagagagg ttaagcaata agcccaatga
58921 ctcacagttg aaaagtggaa gagtgaggac ctaaagccac agccttgact ccctaaagca
58981 gatttatttt ccacagcctg caggtatgac cacagagaga aacgacacgt gaagaagagt
59041 gctcagtggc tgtgggaagg caggaatcaa tgtggatgta cttgcacatg ccctcctcat
59101 cacacgctga acaaattctc attattagac catagaaaag tttgttttta tccccatctc
59161 tctgtctctg tgtaatgtac taaaaactga cccacgctaa cggaaaaaga tgctctacat
59221 tttagcccca tgtcagccat ttccaaaaaa aaacctcagg ggccttccct cttgaagttt
59281 ttgagtc.cca gtttcctcac ctataggatc agggaggtga tgtgtttctg tttcatgggg
59341 ctcctattcg atgtacagca gagggctctg aggcagttcc ccagaataac cttgtacaca
59401 tgactccata tctctgaaca tccattttct caggtgtaaa acctaccata taaacctact
59461 ggtcatttta ggaatccaaa ccaaaataag atcgtgttta taaatatgct ttacaagctg
59521 gaaagtgctg tgtcaattta ggctatcttc caccctatca tttgggacta ccacaatgcc
59581 tcatttctag caatgctttt caccccaatt acaaatgtcc cttattcccc attgggtcag
5&641 atttttcagc taccccaaga gaaaacggaa ataaaatggg cccagtatag aactctcttt
59701 atcctgcttg gStacagaga ggataggtcg caaatatcac aacccaattc ctatttattc
59761 tctcagagtg ttttgaggct gaaatacata agcttgcgtg ggtagaagag gggacccttg
59821 tgggtgatcc taacagtggg gcatccaaaa ggaagagctg tcgccattca tccagggata
59881 catctgccct gagagtggct ctatcccctg cctgctcccc cgtgccaagg agagggtggt
59941 gatatcaagg agtacaattt accaaggctg agtgagttct gcccaSggac ttcatgctcc
60001 gccctcttcc ccactatcca catagggatc cctggacatg ccattttcag agcaaccagt
60061 tgctacattg atagtaactt gacatgtttg atcagcttaa ggccaaaact tcgggttagg
60121 aaatacatat gcaagccaag gtttcacatt ctcccaataa tgctcacttt gagtgattac
60181 ttcctttgaa ttcgacacca tctcccatac acattgtttt tataactgaa aagcaatcca
60241 ccaaccaacc aatgactcat tcaatcaata catgaaataa ttgaaataga gcatacttaa
60301 caggcttttt tcgttaacag taaacatatt tgtaatttac tagatttgtt tttttctgta
60361 acttYaaatt tttgtgcaat gaacatatta tatttattta attattttta gagatatgtt
60421 agtttagtta gttattttta gagacagggt ctcactctgt tgcccaggct ggagtgcaga
60481 ggtgcgatca cagctcactg cagctgcgac ctcccgaact caagtgattc tcctgagtag
60541 ctgggatgac aggcactcac cacacctcac taatttaaaa aaaattattt gtagagacag
60601 agtcttgcta tgttgcccag gctggtcccg tacttgtggc ctcaagtgat cctcccacat
60661 tggcctccca aagtgctggg attacaggca tgatccactg catctggcca tgatatttta
60721 aatataagac aaaacacact ttcRaaacag tctgacattc tagaagtctc tgtgacagca
60781 tgcacatgcc aaagtatttt ctctccatga ggatcacggg cacgttagtg ccaatccact
60841 ctctttctct gtaaccagca tcaacatgtc tttgtaatga agagctgctc attggataat
60901 gtgtttataa agtctgtgtc atcctcttca agtctaaaca caggcagctt tctcatagca
60961 agtgggtagt taacatgcca agtcaatcaa taccagtgtc tcccactccc aaccccagta
61021 ttttctcacg gagctgaaaa caaagacttg ggagctgccc ggtgcagcca aagcacagat
61081 aaagggactg tgactgtgaa agtccagcag catgtggcca ccttctatgc tgtggaagga
61141 ggaagccaca gctgcctggt gcagtgggtt gaactgtgtc cctttaaaag atatgttgaa
61201 accctcacat ctggtatatg taaatgtgac catatttgga aatagggctt tgcagaagca
61261 atcaggttag atgaggtcat taggatgggc cctaaatcaa tactcctagt gtctttataa
61321 gaggggatct gtggctgggc gtggtggctc acaccagtaa tcccagcact ttgggaggcc
61381 aaactaggca gatcgattga gctcaggagt ttSagactaa cctctgcaac atggtaaaac
61441 cccRtctcta caaacataaa aaaaaaaaaa aatagccagg tgtggtggtg cacgcctgta
61501 gtcccaccta cttgggaggc tgaggtggca ggatcacttg aagccagggg gcgaaggttt
61561 cagtgagctg agattgcacc actgcactcc agYgtgggtg acagagcaag actctttcca
61621 agaaaaagaa gagggggtgg gggtttgtac acagacacac cacaggaaga cagccatgga
61681 agagaggcag agagtgatgc tgccatgaga caagaattgc ctggggcttc cagaagctgg
61741 aagaggcaag gaaggatcct cccttaagat ttcagaggga ggatggccct accaatacct
61801 tgattttgga ctttcagctt ccagaactga cagataataa atttctgttg ttttgagccc
61861 ccagtctgtg gtaccttgtt ctggcagtgc tagcaaacat acacacctgg gtttccttac
61921 aattttgttt tgggaaggcc gccctatacc agtgactttt gaagaggcta cattctcttc
61981 tcctgtgagt aggagtagca gtaaatattg acaagaaaca aagaacctaa caaccctcag
62041 aagagctgag aagaggaaag catgtgcagt atgcccatcg tcattttctg gctgtgaagc
62101 atctattcct tcttcccaca gaactctgat ttcctttgca gaattaactt ctctcctact
62161 gtatttaggc attctggggt gataaataaa aaatccagga ctggcgtggt ggctcccgcc
62221 tgtaatccca gcactttggg aggctgaggt gggagaactg cttgagtcca agagttcgag
62281 accaccctgg acaacacgtc aagacctggt ctctacaatt ttttttttct ttaaattagc
62341 caggtatggt ggcatacacc tctggttcca gctactcatg aggctgaggc agtaggattg
62401 cttgagcccg ggaggtggag gctgcagtga gccatgttgg caccactgca gtccagcttg
62461 ggcgacaaag caagactctg tctcaatttt tttttttaaa tgtataattg ctttaatatg
62521 ctgctgaatt tcgtttgcta gtatcttttt atttatttat ttatttattt tattattatt
62581 atactttaag ttttagggta catgtgcaca ttgtgcaggt tagttacata catatacatg 62641 taccatgctg gtgtgctgca cccactaact cgtcatctag cattaggtat atctcccaat
62701 gctatccctc caccctctcc caccccacaa cagtccccag agtgtgatgt tccccttcct
62761 gtgtccatgt gatctcattg ttcaattccc acctatgagt gagaatatgc ggtgtttggt
62821 tttttgttct tgcaatagtt tactgagaat gatggtttcc aatttcatcc atgtccctac
62881 aaaggacatg aactcatcat tttttatggc tgcatagtat accatggtgt atatgtgcca
62941 cattttctta atccagtcta tcattgttgg acatttgggt tggttccaag tctttgctat
63001 tgtgaataat gccgctgtaa acatacgtgt gcatgtgtct ttatagcagc atgatttata
63061 gtcctttggg tatataccca gtaatgggat ggctgggtca aatggtattt ctagttctag
63121 atccctgagg aatcattttt ttaaaaaatc catgagcctc actgcccaag acagccaggg
63181 tacctggctc tgatagcaga cggcctctgc tgaaagcctg agtcctRagc ctgagttggg
63241 ggaaggagaa gcagtcagag ctcattcccc cagggagact ctcacgcctg cttcctatgc
63301 ctctacagca cctgcttctc gagattttct gacaatttta agacaattct atgtcatccc
63361 agcaattcct gttggtgcaa gggttggaat cagtttctgt tgcatgtaac ccaatagccc
63421 tggctgatgc aggaggaaaa cctcaacagc ccagccaccg agcagctgta caggctgggt
63481 gtgcacttaa cttcttgggc ttcagtgcct caccaggcaa gttacaccaa atctcttccc
63541 atgcctagga ttagaacatt tatgattcta aatgtttctg ctataagacg taaattatct
63601 aaggctcaga catggtgagc tgaactgcag gtgaacagca atcctgctcc ggcttgtccc
63661 taagtcaagc agggtcattt gaaacttttg tctttaacaa ccccctgaat acaaggatat
63721 gggagtgcca tctcagttcg ccggtcacac tggcgcagaa atgcctaatt gtggcatatt
63781 ataacataca gcaaactcaa agcttaagac gctttgaact ccattctgca gatagatgct
63841 gagttaatca caattgacct tccatgtagc aggtgaaaat agtgaacatg tactaactca
63901 cacttttgga gggtagagaa tccggaagcg gcttagctgR gtggctccgg ctgagggtct
63961 ctcaggaggc tgcagtcacc tcaaggcctg actgggggag agtatgtttc cagacttggc
64021 tcctccccat acagcctctc caccagctga gtgtcctcat ggcatggcca ctgtccaccc
64081 cagagtgagg gattcaagag ggagggcaag agagccacct gagtcacagt ccttttgtaa
64141 tctaatttta gaagtgccat cccatcactt cactggaagt aagtcattaa ggaaagagga
64201 aggaagtccc acctcctgag ggcaggagta tcaaaaactt gtacacatgt ccttaaaccc
64261 cccacagtgc cataaatagc tgaacttttt atccaatctc cgatttatac agtgacatga
64321 ttcaaagatc aaatgtatct tattaaaaaa aaaagcagga ctaggaggca aaagactgtg
64381 gctttgattc aagttctgaa agcagctcac tatgtaattg gggaaccaag ataactgatt
64441 ctccaagata cttagggaac ctctctgcaa ctatttcttc agctattaca ctttggccaa
64501 gtgctatgct taacacacat gacctcaccc agtccttttc acattccagt gacgtagata
64561 tctgaatcct catttctcag ctgcgaacac tgaggcataa agacactaaa tcacctatcc
64621 aagagcaccc aagataaccc aagataagga gtcaggatgg ggccctaaat ctgtctctca
64681 agcttcagat gtagattggc atgtgaagag actcagaaaa gaggaaaagg tagaaataaa
64741 agtgctacaa tctgataatg cattaccatg aaccataaat aataacagag ttaataaaag
64801 aagtgagaac aatagtaatg atggtgctga tgatgatgtt aggcatgtgg gtgtcacgtg
64861 aggggccagc tgactttcta gtattcactg tgcatctctt tccttgactg ttcactgtat
64921 ctctgaggac gatgtgcttt gtttggtgcc ctgtacaagt atatgagcat acttacggtc
64981 tccacttgaa gaagatgaaa gcattgaaaa ataaaatggt gccagacagt taggccagta
65041 gttaggaatc tatcatcttc agtaaaatcc tgaacttcac ttctccaaaa gtcaacatta
65101 gtctaaatta aagatgttgt cagaaaagaa gataatgcat cacgtaaaac ctacctagac
65161 atactgcgta cccaacatcc ttcagtaccc agttcagtct cctgtgaaga cgccctgaca
65221 tctgcaccct ccagccttgt tcacacatcc gctctagcat ttcccacttc ccatctctac
65281 agtgggtaaa caggoagggc gggaaaaatg tcttactaat ctttgaatcc cataaacaca
65341 gcaaggaatc tggcataaag taggcatttc caaatattta ctgaatcaat gaaccactcc
65401 tgagattact atgtgtaatg ggtgtgagat tatgaaatca tcatttaata aacacacatt
65461 tctgagattc aaactcttgg cttaaaaaag gtttaaagat ggattgattc agggaaacat
65521 ttaaagttac aacattgcta tagactgaat atattcttct aaaattcacR tattgaactc
65581 taatccccaa tgtaatggta tttggcagta tggcaactgg gaggtgttca taagggtgga
65641 gcccttatga atggggttcc ttttaaagag gccccagaga acctattggc cgttccacca
65701 cgtgaggaca cagtgagaag aaggccatct atgaaccaga agacaggtcc tcatcagaca
65761 acaaatctgc tggcaccttg atctgagact tctcagtctc cagaactgtg agaaataaat
65821 ttctactgtt tataggccac tccatttatg gtattttgtt acagcagcct gaacagatta
65881 agactgtgat actgccagga actttgataa ttattatctg aatttatcca aagaaacact
65941 gtgaaattgc cttcatttta caggtgagga aactggggtt caaacagaat aaataatctg
66001 ccctgggtcc ttcatggcca gtcctgaagc cgtgtgtttc taactcctgg tctgacatgt
66061 gcctgctact gtgtgccctg aattcaagtg ggcaaattca ggaaggcaca ctgaacttgg
66121 cttgatgtgg actgataatc aggtactaaa tggctgcttc tttttttttc tgtttcataa
66181 tttacaccca gatttggaaa gatttggaag cagattacat ctttatcacc caaagtatgt
66241 tttcagaaag aatcttgttt ctgagaagca taaatttagt ttcaaatatt tataaaaatt
66301 actctaaata aaagcaaaac tccatgaaaa agaaaatgca ctggcataga gagaaaagcg
66361 agaagcgaaa ggaagtaaga ggaaaatgag agatggggag ataggatgac gccgtgggga
66421 aggagaaaga aaatcaggga aagatttaaa aagaagataa tatgtactta agttaatcac
66481 agatgataga aaatcttttc aggaaatgta catctgaatt taaaaacagc attctttagt
66541 tccacttcat caagcaaaca gcagaatctg caaggtgtga atcctcccac ttagggctgg 66601 gccttaatag actgtgtgtg tccagtgcta gcctcggggg aaccagctct ccatacacat 66661 ctgttgaatg gaaatcaatt ttcctcaagc aaagaggcag ctctgtctct cgtaaacaag 66721 cacctccacg tctgcagtca cataagactg attttaatga gcggggtgtg aggtcacgcc 66781 cgaggtacat ccgcttatcc aaggggccca gacaccccac caggaaatca aatttctcag 66841 tacatacctc aatgatgaac cttcctttta gcccctaaag gtttctctca Wcctttttgc 66901 atttggtttt tggccaagca ggctggaaag tataaaagcc aaaagaggca ggggtggagc 66961 agccgggcaa gcaggaagaa gcatgtgttt tctggcctcc atctccccag caNttccagc 67021 atgttccgta attgggcagc ctggcctcac cgtccaacag gcatcctcat gcagcccgYa 67081 gtttccatgc tgctcactgt aaatgtctga tatctgcacg tcctctgaga gcacagccca 67141 gaggctgagc atgctcacag acacccactc agcacctggc cctcagaact gtgctgtccc 67201 cagcctaagg cctcagggtc caatgcccaY ccctcttgct cctgcagaaa tctaggctgc 67261 aaggtgctcc taatctctgt gtccctccag ctgcaatagg aaccctagtc acaacagatc 67321 ttatggccac atgagcaagg acgggctcct gccaaggcat ctggtagctt cctgcccagg 67381 taaggtgggc ctccctggtg ggctctgtat ttgttttgct gctggacaga agagctcctt 67441 cttttagaat catttgcagc agtggcctct acatactggt tggaaatcta taattatatg 67501 gaataaatag acacaaaatg agaacaagaa ggattgaagt gatgaatgtt tcacacagct 67561 aaagttactc Rttttcaaag aatgtcctct gctcaataat ctgttcttcc tacttcgtgg 67621 tgtgaaaaca gcctttgtgt tgtgaaatgR taatgacagc agagaatgct ctgggaattg 67681 tttgctattt tccagtgtcc caacttaccc cagccctgcc atgcccatat ttggacaaat 67741 atctggtatc cctatgttgg tatctgcccc acagtatttt tttggagggg agggggcggt 67801 ggggagggcg gatatttttt ccatttctta ctgccaacaa tgtgggaact actgatttgt 67861 agtaattaaa gactgtgaag atttccaaag aRagaaatgg aacacaaaat tgtgtcaggt 67921 gtggcggctc acacctgtaa tcctcgcatt ttgggaggcc gaggctggca aactgcttga 67981 gcccaggagt ttgagaccag cctgggcaac acagcaagac cctgtctctg ctcaaaataa 68041 aataaaataa aataaaataa ttagccaggt gtgaaggtgc aagtctgtag tcccagctac 68101 tcaggaggct gaagtggaag gaccccttga gcccgggagg tcggggctgc gtgagccgag 68161 atcacaccgc tgcactccag cctgggcgac agtgaccctg tctcaaaggg aaaacaaaca 68221 aacaaacaaa caaaaaacag aaacacaaaa gcatcagggt agctaaaaag aaatggcacc 68281 aactcatgaa cttgcacaga agcacggagt gtattcaact caggcgactg agggtatttg 68341 ggcataagaa acaggtcgca ggacaacagt cacagggctg acactgaagg caggggacag 68401 cgagagttct gcccttgctg actgagaatg gcactgaggg caggcgatgc agaggggcca 68461 gagaacagag caggagctgg cagatgagaa aagtggaggg aaggtggctc tcttccaggg 68521 tttttgtgaa tggatgggtg gggagtattt cctccacatt ccagaggagg gaggaacagt 68581 gacaaggcca gagggtggag aaaggcttaa ggaatctgtc tctggggaca cttcacaaat 68641 gcacagcaat ctcYgcatct cttcacgcct gctcaaagaa ctgctgcctg ctatcagcaa 68701 cgcgacctgt gttttgtttc cattttgttt actgttgttg ttgttttaaa aaataaccaa 68761 ccttgccggt ggcgtgctgg attttttaaa ctttttcctc ttcactggca cactcacaag 68821 tttgtttagt gtaagtctga gaatccaaag gggttttatg tgcctcacat tcataaatcc 68881 tcaactgctt ttcaaccaaa tttattgccc ctgaggattg agggtgaatg gaagagtcaa 68941 aaggaatctc ctgggctaga Nggagaatgc acagagctgt gtgcacacaa tgtcaaatat 69001 ccagatgaaa agtgaaaact tgagYaaatc ctccagatgc ttccaaggac ctgaatgctt 69061 ggtgagtccg cagaaatacg tggatgctta atggaacttt gcaccgttag tagcaatagt 69121 tagcactgtg tttgtacagc ccaccacgga attcacagat gtgtctagac ttaactttgt 69181 caatcctgat aaattaaaaa tgatatgtta tgcctccctc tagagtcatg accagcatct 69241 tcagattaga ctagaaacct ctaatctaac agattagaaa actatgcctc ttctggggag 69301 acctagcccc tctggcactc agcttggtga gctgatggcg ctttccgcaa actggaaaga 69361 tagactcttc ctctgggtag cttggtgagc agagtatgtg ttcaagttta gccccttatt 69421 tctgtccttt gtgcagtaaa cacccagcac aactgtacac agacaccctg cctccctcta 69481 cagtgcccaa acacaactca gctagaagtc tgaccatttc aaagtaagag gataggaaat 69541 aatacatgat taagacttct ttagcataag ccacacacac aaatagattc cacaattaca 69601 gagggaaaaa gaaagagaaa gggaaaggaa aagagtgaag ctgccctaaa atggcttacc 69661 ttagcataat aaaaccatgg gatttcagag ctggaaggca tctttttaaa ttatctattt 69721 atctattgca accatccctt gcaagaatcc tttgcacgac gtccccatcc caccctccac 69781 cagcagtcat ccagcctaac ctgaaacacc ctcccacccc tgtctctgag aactggaagt 69841 ctgacgccat caccctcctt tcacactgct ctatgcacaa gaaatcctgt cccctccacc 69901 ccaccacact ggcctccctc taacttttgc ccagcccatc aatgcatctt agcgctgcat 69961 tctgacgcca cctacattaa gtgtaattgc ttttctatgc gatagctctt taagtactgg 70021 tagacattta accatggccc cctacaatct tttgttttcc cagctaaata aaggcacctt 70081 gctttaatcc aacccctcct acaaaactaa gttaactgcc agcatccatg aacaaatctg 70141 cggttcatca cagtcactac agggcagcca agagctggat aaagaaagac attgctttca 70201 ctgacaggca accccatgtt ttggaaagta gaattcctgg aagtctggca cctcaggaaa 70261 tttaagtccc acacaaaccc catgggctat aataagatat tgctgatgtt ttggctgata 70321 tgcaccagaa agccttggga ggaaagtggc ctagagactg taatgaatga gagcataatg 70381 tcctgccctt gaagccacac aatYagaaag tgagaggact acagaaaaac cagcagggca 70441 ctggcatttt ccagKtgagc attctgagct gacagtgaaa ataacgaatg aaacacggca 70501 gagcagaagt ggactttatt tggaaaacct tgttttcccg gaactgaagc taacctctgg 70561 gacgagaggt ttcagaacaa tgaagaatca Mcaaataaga tgaagagacg cctactgaca 70621 aagacaaatt ccataccctg gaactggaca tcttcctggc agagggaaac atcctgctct 70681 aaaatgggca caatcagcag Yagttatgaa cgtcggcttt tccacttgca aaagaaagat 70741 tttaactttg cctgacagga aggaacactg accaagaaaa cagtaagtta tggattactt 70801 tgctacggat gctggctata cctctttagg atactttttt tatagaaaac caagcattac 70861 ttttctgtga atggaattaa gtgtcaggaa tatgcatatc tttcttatat aaggctttgt 70921 tttattataa cagcaagaat catgactgat gcctgaaggc ggccacagat aatgccaaga 70981 aaaaatgggg tagtaagtgg aagaatctca caaaaaattc tgtgtaacaa aaagacagag 71041 gaaccaacct ctgtcttatt cctttcttca aatgtttcca aaatttattg ttagtatctc 71101 catacaaata aattaatttt cagttttata gtagaattca attttaaaaa ttccttttac 71161 aaccaacttt tctaaaaatc cagcatagga tacccaaata aaggtgcatc tccattcatt 71221 catattttca ttcaYtcatt cattcaaact tcaacagata ctgattggtt ggatgcctat 71281 tatgtgtctg catcatgcta ggcattcaag aggccgtgtc agcagcacag gcaacggaag 71341 tggacattcg ttatttccac atacaatcac ctactttaaa accatggtaa gtccaagaaa 71401 ggacaagccc acggtgtgaa gagaaagcca taataaaccc ggtaattcag aacagaggtc 71461 aaaaacacat gccctgcaca gcatctacaa aacacaggaa gccaacactt ggttttgtga 71521 ataccacagt gttgcaatta tcttaagctg tttttaagtt atttccaaaa tattattttt 71581 aattaaagta gagcacatac taactttaaa tgatagaggg aaaaggaggg atttgcaatt 71641 aaatgaataa ataatggaac cgacacattt tgtttctttt agtaaatggg ttcctgaaaa 71701 gcaacatcaa cttattcagt gattgtctgc cccaaggaca gtagacaccc agtgcaaaat 71761 ggctcccagc tctggttggt ggcacatgag cctgtggtgg tttgagaacg acaagagtcc 71821 agaacagcac gagctttgga aaccagtcaa acttgggttt gaatcccaac tctaccacta 71881 gagatgcaaa ctctggaagg ctattcatct tctgtaagcc tcaatcttct tctataatta 71941 ctaccagagt aggaaaagca gtattaaaga ttattaagga cttactacag ggttggctga 72001 aataaataat aagtaaataa caacttgcat agtctatcaa cccttatcca cactaagttt 72061 tgccagaaag tctcctgcca agagtgagac tggactgagg ccgaaaacaa ggggcaaggc 72121 atgcatgcag agtgctggga tcggatatga gtaccagcga actacagcat aagatctgct 72181 caggtgaggc aagttcagca gtgtgagagt gttctcatag gtgccaagtt gcaaggtagc 72241 tcaaggggtc agatcaaggg gtctggacgg tgttgtcagt gagtagctat ggtctgagca 72301 ggaaggtaat cttgggggca tttcaagaag attaacaagg cagcagtatg aaggacagac 72361 tggagaacag aaagtctgaa ggccattacc aYagcccaga catgaacagg acaccaggaa 72421 tagaatggag gtgatacttN aagaaactgt gtcattttct atgggccaaa ggcaggagag 72481 caggcctgag gaaggtagaa aatgtttcaa atagccaata tcaggtgcat gagacacagc 72541 aacaagaaat aaagttctag agcacttaag cttccctgaa gctaagcacc ttttctacca 72601 gctagctcac ctagctctgY tctttcctca gctcgctctc tttagtccag gaaaaggagc 72661 acagaaacca ggtgggatgg cttaggtttg cacagtctgt aacgtgatca tgagcattgc 72721 Rggtaagctg gaaaaacact agatgaccca ttgtgagcag tgcacccggt atggggaatg 72781 ttaaagcaag atccagaaag tggccaccct agtgcttagc tccagggtgt aaagccctgg 72841 acatccagga aataggagcc caagaagagg aataatagRa aatggaacca gtaaagacag 72901 acacagagct tgtgaaggac aaaaaagtgt gccagatgct gaagactccc acaaacaatg 72961 acaggggaga ttttacccca ctattcactg catgacagga atttcatctg ccgtctatga 73021 taatatcatg gactctttct ggaaaccaac gctttttctc caaatgcatt atgcctcact 73081 gtgcatatct gggccccaga agactgtgtc cactgttatt ataagcagga agccagccag 73141 gtatcttcag ttgatgagga acaagatttg ggctcaactg ttcagtgacc tacttagctt 73201 tatttggtta tatgaccaca gaatatagtt ctatggttat gggagcaaaa acacagctag 73261 atagaatgaa taagatctag tatttgatag caaaacaggg tgactacagt gaacaataac 73321 ttattatata ttttaaataa cgaaaagggt ggaattagaa tgttcctaac acaaagaaat 73381 gataaatact tgagatggat acctcaatta ctctgatgtg attattacat attgcatgcc 73441 tgtatcaaaa catcacttgt acttcataaa tatatatgcc cattatttcc cataacaatt 73501 aacattaaaa ttttttaaat taaatataat atacttctaa tcccaaccaa cattctttca 73561 actaataact ataaggatgc ctgacattta ctgaatgtca gaagaatcta tgggctagaa 73621 acgtgttctc cctatgaaga aggtaccact gccatcccca ctgtagaggt gcatatctgg 73681 gcaccaggag actgtgccca catgagatca tgcatcccag cctgcccaag agagaggaga 73741 cattcagtta aaaataaata aataaatagg gccgggtgta gtggctcaca tctgtaatcc 73801 catcactttg ggaggccgag gtgggcggat cacttgaggt caggagttcg agaccagcct 73861 ggccaacatg gtgaaaccct gtctctacta aaaatacaaa aattagccga gcatggtggc 73921 aggagcctgt aatcccagct actcactcgg gaggctgagg caggagaact Scttgaacct 73981 gaaaggtgga ggctgcagtg agccgagatc acaccactgc actccagcct gggcgacaga 74041 gcgagactct gtctcaaaaa taaataaagc ccaaggaatg ccagggatgc tcgtatgatg 74101 tctgcatctc tggtgagtaa gaaatacaga agtacRgagc agtgtgaaga agaagaggcg 74161 agaacgaccc ccggaccgac caaagcccgc acaccaccac atcctgtgcc cagcacctac 74221 gtcccgtcgc tgttgttgcc agcatgccca agagaaaggc tgagggggat gctgaaggag 74281 atgaagccaa ggtgaaggat gaaccacaga gaagatccac aaagttgtct gctaaacctg 74341 ctcctccaaa gccagagcaa gcctaaaaag gcccctgaga aggtacccaa agggaaaaag 74401 ggaaaagctg atgctggcaa ggaggggaat aaccctacag aaaatggagt tgccaaaaaa 74461 gaccaggccc agaaagctga aggtgctgga gatgccaagt gaagtgtctg catttttgat 74521 aactgtgtac ttctgatgac tgtacagttt gaaatactat tttttatcaa gttttataaa
74581 aatgcagaat tttgttttac tttaagctat gttgttagca caYagaacac ttcattgYtg
74641 tttttggagg acaggatata tgtccctaat agaatgtctc tgaagctgga ctgatgtggg
74701 gaaaacacct ttccctttcc cttctagttc tgagagactt cctcttggct cccaggagga
74761 ggggggactc cctgactttg atacacatgt ccaccttggc acaaaaacct tgtgggaggg
74821 aaaaacaaat ttgattttat gtcctcttct ccctttccac ctttcagcat aggcttaact
74881 cccttaaagc cagacatctg ttgggacatg atccccagtc attggttacc agtgtgtcac
74941 acaatctgga ctttccagtg atggcaatga gatggcaccc ctcaaaagag cagtggttcc
75001 gtttctagat tgtggatctt cagataaatt ctgccatttt cattccactt cctgaaagtc
75061 agggtcagct tgtgaaaagt tgttaaacaa catgctaaat gcgaaatgtc aaccctcact
75121 ctaaacttac cctgttcaga gcatcagatg aagacttatt gggttttatc ttggctttct
75181 gattattggt agtccataga agaagggagt ttgaaagttg ttgtatactg ttaacgactg
75241 tctacccatg tcctgcctga aatacgatga ttgcttatgg aacatatctt ttataaagct
75301 aggtacggtt tggcttggga aaaaaaaaaa gaaatacaga agtgctttct caatagcagg
75361 tgtgtgtgaa ggattgaatt ggttattagg gtaaagaggc ttaggaaaaa gaaatgtgtc
75421 cttcagtcac tgctaagtga cacatccgca ctgtgctgtg aagttccaag aaccctaagg
75481 tcaactggct ccttgccagt atatacctgt attaactctt ccagaaatct agtggtgtag
75541 gtatgtatgt gtgtgcaggt cggggagagt tttttgtttg tttgtttgtt tgttttgtga
75601 gatggagtct tgccctgtca cccaggctgg agtgcagtgg tgtgatctcg gctcactgca
75661 acctccgcct cccaggttca agcgattctc ctgcctcagc ctcctgagta gttgggacta
75721 caggcatgca ctaccacact cagctaattt ttgtattttt agtagagagg gggtttcacc
75781 atgttagcca ggctgatctc aaactcctga cctcaggcaa tgtgcctgcc tcagcctccc
75841 aaaatattga gcttacaggc ttgagccacc gcaccgacca ggagaggcat aattttaaaa
75901 ataacagagc atttgcaata gtgaagaaat aagaagagag aaaagggaaa aaaagggtag
75961 gagacaaaaa tgtaaacctt tttcttgtgt tggccccaaa taattttcct actttttttt
76021 tttttttttt tttttgagac ggagtctcgc tctgtctccc aggttggagt gcagtggtgt
76081 gatctcagct cactgcaact tccatgtccc aggttcaagc gattctcctg cctcagcctc
76141 ctgagtagct gggactacag gcgccctgcc accatgccca gctagttttt gtatttttag
76201 tagagacagg gtttcaccat attggccagg ctggtctgga actcctgacc ttaagtgacc
76261 tgcccacctc ggcctcccaa agtgctgaga ttacaggtgt acccaccgtg cccagcccat
76321 tttcctacat tttcggaaac ttcaaattac cacagatttt caatctctga atggaagcca
76381 atataattca tctcttcttt tctaccaaac tcctgatagt tcaggtgacg tggatgttac
76441 attaactctg tcaggccccc gggctgctat taagtgccct gtaacactga ggtcctaMac
76501 ctatttccct acaactaaga gtttttgtct cccaaagggc caaacttgca cacagcaggg
76561 acacagtggg gcagttagtt gcttgggaaa gagaaacaaa atttcttttg agagtgaggt
76621 tatKttaagg tggtcacagg tatgcagagt cagttttatg ctctccgtga tttcttatcc
76681 cctctgagag tgggaaggag ttggcataga gatttttatt ttcatagcac attatataat
76741 ttggggttgg tggcaagtgt taacaactgt catactaccc acggctatat taaaaataag
76801 accaaaatag agattgttgt gggttgYctt gggttttccc tcagaagtcc tgaggaagaa
76861 agtacctcaa atgaacaggg agggcatcat cagctcacag agcagcctgg gcaacaccaa
76921 gaccagacag aaaccctttc caagcaacag agtggaaaca ccaacaaacc cttttcatga
76981 gacacacatt tctgcaagac ctatcttgac agaagccacc aggtcccaaa catgtcacat
77041 gtatgaaaaa ttcaaattca aacgtggaga agagcagttt tatgggaccc gccttcaggt
77101 acagctccct atttaagtgc tgagttaaaa acctctgcgt tttgaacagc agcacacgag
77161 ctcatgtgat ttaatttatg tgatgaaatg tggatgctgg gtaaaattca cccaggaaac
77221 agaggggaga agcaggatgc tcaacScata aacaacactt atcctcagaa attttggccc
77281 tttgaaactg ggtgaataaa ctaaataaac tgaaaagctt cattaatacc cagaggatca
77341 ggtccaagtt ttagatctgc aaaaagaaaa aaaaaaaaaa aagaattaaa gctttccatc
77401 ccctttctaa atacgtgggg atctgttttc aacccattcg taaaatctat ccatttgttt
77461 ttattatatg aacaacttaa aaattgccag acagaccacc tctgatgcac gagggcacag
77521 cattggttac cggagatgRc caaagcattc tccaaatcaa attaggtttg cctcagttac
77581 agaggtcatc ttactgtatg ttttcaggaa tattaacaca aaatgtatca aaacactaat
77641 agRatccata aacacagaca gctaaagctc catcactttt aacacgccat aatttaataa
77701 gaggtctcat aagagacctc ttattatggt ctcataagac cgagggtgtc cttttcaagt
77761 tccttccctc tcagctactt acggcaattc tcagaagtct gcaaagttat ctacatcctc
77821 cctttaccaa tgttcagcca tacgcagata tcacttgtga actaaacagt gtctttcctc
77881 cagggacagg gccaaggcta caacatgctg gtgcacactt taaattgtag gtaaacgctc
77941 aacctatggc agaattccct attttgtggg agtcccagtt ctttaaacta attctaactt
78001 ttggtgttgg ctttcaaaaa ttactatgtc aaggaatatg cgatgttgag atttcactca
78061 atagattggt cagaaggaca ggagggagga gagagaagaa aagatagtgg taggggaagg
78121 agaaggaaat gataccagca aaaaggccag ggctctggat tcctggatgc ctctttgagc
78181 aacgttttca ttcgcaccac ctaggtgcac ccttatatgc ctctggtcct ttatttacaa
78241 ggctgtcacc tgtctgacag gtagttttac atgttttaca tgttgcagta ccaaggacca
78301 gacgcctttt aggaacagtt ttgagaatga caagtcactt agacactcat tgccatgact
78361 cagttttaag aaaaatgacc aatgactgag cattcctaat ggggaagctt tcccatctga
78421 tgggtgcgat tctgacccaa tgaaatgggt gcaggagaga agacattgag aactccagct 78481 ttaaaagagg ccaggactcg ttagtgaaaa tagttttgac ttgttagatg cctgcttttg
78541 ctgggggaat ggaacaaatg atgtttcagg caggagctgc cagcacatga acctccttct
78601 agcaaagtgt atgatctcag aggccataca tgttcattta cagtagagtt tagattaaag
78661 tgataaatgt ggatctggtg tacatttaaa tgccattttg aaagaaaacc cagctcgagg
78721 ctttcccacc ctacatatca ctgcagattc cgtggtcagg gagatgctgg cccttgagta
78781 gctgccgctg tctccatgga aggacggcac catagttaat taaatgtcca ctctgacggt
78841 aaaattcatt atctaacact gcaagtgaag ttatcagaat atacttgatg ggccaaagct
78901 gtcagaaggc cctctcaaac acttaacagg aaaagataag atgaatttgc ctttcataca
78961 gggatttttt tctttttaat ggaggctcaa aataccaact tcgggtttgc aaatattctc
79021 atccccttta aagcttaatc atcttaatga aatacactca agggcttttt cacctttcca
79081 ttatttttta ttttcttaat gtgcatgtta agtcccaaat agtttccacg gatggcagcg
79141 ttgagttcag agtcctaact atggactaag cagtttttat ttcctttctc actgaattct
79201 ccacagcaga tctgaaaaga caatatactt atcctcaatt ttcaggtgat ggatacaaag
79261 tttagagaaa atgaagagct agaaagtggc agtgccatga ctcaaaatgg gccttctaat
79321 cacaccaagt ctaacacaca gcaactagag acccctagcc cccaattttc agtgtttttc
79381 ttagattggt ttttgattca cgtggggaat ctcagagttt ctgtggggcc cagggggcca
79441 tagtttcttt tcgctcttaa gcatcccaaa ccatccttgc cagcaccctt gattgttctg
79501 cttcactaag aatgtgaagc tgtcaaattc aggccagaat gacgtgatca tcttgacacc
79561 ttgacactac accacttccg ctgcagccag ggggcccagt ggccttctga tttccaagtt
79621 taagttctca ccagcttaac ttgctcattt gacacagtaa gcatagaatg acaggctgtc
79681 accactgttt aagagctcct aacgtacatg ggatagctat gggtcaaaac ccaacaaatc
79741 actgttttga aagagaaacg aactctttca aactctttca aactcagcat cgtgaaaccc
79801 aaaactatga agggaagaac accatttggg tgaccctttc acagttcagc aaggctcctt
79861 caaccactct ccatataagc ctcctgcaac cttggaggca gccagggctg taggaggcag
79921 gcagggctga taggagcttc cctcacctac aagtgacaag gctcagccaa gggaaggagg
79981 gacaggacag agcacaccaa caagggaagc cgaggcctct gactcccgca ctgtattctt
80041 tcattccgac ttctattaga aaaagggatc ttgtcaatga atgtgtcctg tgtctacatt
80101 tctatgtaag caaaacaaga aaaccaaaaa gaacatgtga ggaaagcaat tttcagtgtt
80161 tttcctcgac cggtttttga atcacgtggg gaatctcaga gtttctgtgg ggcccagggg
80221 ctRtagtttc tgtttgctct taaccatccc aaaccatcct tgccagcacc cttgattgtt
80281 ctgctacact aagaatgtga aactgtcaaa ttcaggccag aatgacgtga ccaagaccag
80341 ctgtaatgta cactctcttc ttaaagtaag tgctggggtt tgggaggttc ttaccctagg
80401 cattaaggct tgggagtacc tgcttgttat atcagctggg tcccttccct gcattaaaga
80461 gcaaaggtac ctcaaacaga acagatagag aactgacaca aagtaaaata ttcccagatt
80521 tcctatagaa aaatacagtc acttttctaa aaatgccaca agggtgacgg tttggcagga
80581 gcatctctta acaatcagca gaaatctact aaatccctct taaaaggtcc attctaaaca
80641 acattccaca gtgacacagg ggcctgactt tgatttctga ataatttgac tagaagcaag
80701 gaaagtcaca acaacattaa gtgcaataac tttctacccc ctKtttggtt cccagtgtga
80761 aatttgctga aataaaactt gacagcccaa aactcccggg gatgtgttcc agatgaatYg
80821 agtcatccac acaaactcac agacacacgc acacacgccc tggaccctcg cacttataga
80881 gggagttgtc tccatctgta tttgacaacc cggtgccatg ggtctcaagg ctgagggctg
80941 cttatggatg caactttaaa tttaatttgc gggggatgat aattgtgtgg agtggtagga
81001 aaatgctggc gacattttga ctttagcagc cccactgccc tgattaaaaa atatcagctt
81061 tccatatgca agacatttct aaggtggaca ccttacaaat tgttaaggtg caatttgtgc
81121 acagttagtt ctttgtttaa tcgagaaatc tgcccgaact ctgattgggc tgatttctct
81181 aaggccggga gtgaaggtta tttccttatg ccttttggga gcagcaatct ctctaatacg
81241 tttctgaatt attccagctt gtgccgaggt tgaacaggag aacaaagccc tggttccaca
81301 gcagaaagac taactgaact cccagctggg aaggtttagg cagagattta taagtacaaa
81361 cactgaagaa atttgcaagt aattgcatgt atttgctttc cgttgtggga tgcagtaaac
81421 cagtatttat taaaatgcat cggcgaggta ctgtttatta ccccctctcc ttgatgtctt
81481 gtagagggga ataaagtgct tggttctttt catttcttta aattaagggg gcaaaacagt
81541 atttcaaaga atataaggaa gaagctgaaa ttagacatat actctgaatg gctactgcct
81601 cttacttaac ttggaattgg tgcatgaaca tggctccaga tatgctgttc tgatttaatc
81661 ttgtaaagct taactttttt tctacattca taagtttcca tgaatccagt atttggggat
81721 caacaattat cattttctcc catagaattt ctgttattat tcttggattg cMgcctgcaa
81781 acctgagtgc taaataggca catgttcatg ggatataaaa tgacaggctc agaaagttac
81841 agcttaataa gagaatgact taaacaaacc accaccaaaa agagatatta tgttcactgg
81901 ctcctgcatc taaattcctg tgccatcagc tactttgggt tttaatttgc caatgacaaa
81961 aggtcagaag aaaccagaca gaYgactctc tttccctctt cgacttgRat cacacctccc
82021 cacctgcYtc agtgttcccc tcttaatgac agacccaaat tgttctacgt aatactcctt
82081 aacacaatgg tctcaaactt tcgctgcatc agctttgcct aaagagcttg ttaacacaga
82141 ttgctgggtc ccagcccaag agtttctgat tcagtaagac agagaaggtg cacaagaagt
82201 gccatttcta acaagttccc tggggatgca gatgctgcgt gtcctgggac cRcatgttga
82261 taaatgctgg tctagaaagc aaataggcaa aagtttggtg cagtgaaggt aagcggtacc
82321 tactgcccct gcccccaaaa tagcaaataa cagaaacgaa ataccaatgt atgttatttt
82381 agtcaacaat tgttaattgc aacctgctta aatatatggc tgacaacatt atctccatgt 82441 agaattccca agcccctgcc ctctcttcca cattggtctt tgcctacttt tccttccatc
82501 tgatgtccct taaggatctg gatgggtgca ccaaactggg gagtgacagc ttccagtttg
82561 tatgtctgct cttcttagaa atgcaggatc ctcatgtccc cacctcctcc ccctccatca
82621 ggagtgcacc cacctaagca gtaggcaccc attgaatacc agctgctatt gttttaaatc
82681 agaataaaac cagagagcag taacagaagc tagtgtgtgc ttctctgtcc ctcagcacag
82741 caactctggt tatcttaagt tcagcattgg taataaataa gccccagatc ttaaaattaa
82801 aaggtaYcaa gtagccacaa caagtggaag gtttctgaga ctggcaacag agatggcagc
82861 caatcccctc tccccttggc ttgagggcca Ktacctccca ggcttccaca cctgtaactt
82921 taaatcagag aacatgcaaa caagttatct cccaatgtct gtgacatgaa gacaaatgaa
82981 ttagagaccc tagaggaata ttaaaatgaa aaatRctatt tgataaaaat aagccagacc
83041 aggaaatcag atagcatgct tatatatatt aatatatgtc atatattata tataatttat
83101 attttatata tatgtatttt attgaaaaag tcctagcttt cacacagtct ttccagttat
83161 aaaaccctca cctcctaatc acataaaatc agatttttta tgatttttat tagagaagaa
83221 ccatgaggtc tgtaggtata accacaaatt gSccagaagt gatattgttg gaacaggaaa
83281 ctagactgtt ctgtgcctca ggtattcaaa cttcaatcac ttaagagaga gaagagcttc
83341 ataccaNtat gggctctcat gacaaagtgc atataggatg tcaatgtctg gatccagatg
83401 ctcgaccctc atacaatcat ctgaagcagg tacttggtgt caggaaggaa gctgaggggt
83461 taagtgactt acacaagtca gccagcaaag ggctgagact agaatcctgt cctttctaat
83521 tccaaaatca catctgattt tcctccaaga aaccaagtca ccagcaccta tcttttcttt
83581 attaaaagca tgYgcaagtc aagtatattc taaaagataa tcagctggta aaactgaata
83641 aggaaaacca gctgtagtaa tcccctctgt accaaatgct gaatcaKaaa agacacctag
83701 ctttctgata atcagatttt cctagagcct gaacagggtg aaaagttatt gaatgcagaa
83761 tatttttact agaacacgca agagtgtctt ttagacctta cgatcaaaat gtccaaaagg
83821 ataaatctga atattaatta caaaacttgt actacttcaa actttttatt atattctaca
83881 cctctactgt Yttcttatgg ttatcacaag tgctaacaaa atattagcat tcatatcggt
83941 aaaatcaatt ggcaagtcca ttatcgacat tbatttcaaa tgcgtatgtc tgaagtatgt
84001 gttactcatc ccttaaatta acatcttcca ctttgatggg acaattggac agaccaggcc
84061 aacacttttt tcaccagtaa ttagtaactc tattttcaca tttcYggaga gagaattaca
84121 ctcaaccaac atttctacat cctgaatctg ctaatctcag caataagacc ttcctttaaa
84181 agacatgcag tgtcttgacc aaagagtaat gatagaaaac taattcacaa acttggtggt
84241 ttttctagca aatgagagca gtttcatatt tagtaaagtc tatatttttg ttgacagtac
84301 acaccaaaat acataYgcat taaaatgcat acaagggtgt atatatttct atttcctaaa
84361 aggaaattca gagatacaaa aacgtaatta tacaaattat ccttacttct tgttgttaat
84421 tcagtttagg cacaacRctt gagaaataaa taaaaattct ccatagcata aaataactgg
84481 tattccatct ttacttattg gggaaagtac taaattgatg tcatcataaa tattcaattt
84541 tatttttaag taattaccaa aagtaaagtg taattcaaag aatggtctga tgtgccacaa
84601 ctagctgtgc aaatgtacag gatcttttta agacggaaaa tgtaagtgct taccaactgg
84661 gaacttgaag aaattgagat tgacatctga cctttaaaag cagtggttct cacacttcat
84721 tgtttaaaaa cttgttaaaa acacagaaYc taagatccag ttctcaggaa ttctgattca
84781 ggagtctggc acgaagccaa gatgctacat ggtcacacca agattcgcaa ggttggttct
84841 gttactacag tttaaagagt caccttcaga ggcaaggccc ctgatgccca ccgagtcatc
84901 attctgcttt gttcgactct cccaccgcag ctgcctctcc ccactgtctt gtcttgcctt
84961 ccattattta ctcactcatg ggtggagagg agttaccctt aaaatagacc atctcaaaag
85021 gtgattttcc ttttgataac tgtcaatcaa tgtaaacaca ccacaaaaca atcctttgca
85081 gaagtttaga agccaaacac atgcttatct gtagaatgtc aggtggccta tatgactatR
85141 tctttttccc tgactaaact gactacagga gcttagcttg tttctgctgt tattttaaag
85201 attatttcaa atttgtaaag agtctgacct gcagaaaatg tggacgacac ttattgtggg
85261 actgtgtacc agttatggta ctcgttttaa ttctaagtgo tcctcttcct acagaaccaa
85321 accaacacMg gagaagctga gcaaatgcag tcagttggat gtgaattacc ttttagttgc
85381 tgacaacaga aagttaccct gaaccactga ccaagggatg aaaagcgtcc gtgtactatt
85441 agtaattctc agaatcatct ctgtccccaa ccaagtatgg aaagccaagt acagtatcat
85501 ggaaccaaat tcaaatgctg gtctcaaagt tcccgacttg cttgccttca agtgccactt
85561 gagagatttt aaatgacagt gaaatgcttt gttcaactaa aaattcaaag tgtcgggaca
85621 aagtttattt ctgagactca agagatagtt tttgctttag ttgttgccat tggtgctgct
85681 gtgtgtgtga aaaaagtcag aaataaaatc tgccactttt cttttcctaR atgatggtat
85741 ttaaaatagc aagtctaata aaatctagcg gtttgatttg ctcgctcaaa gaatgtattt
85801 ttaagatgaa gtgttccagg tagatgcaag ttatgcagat agatatttca taaagacaat
85861 gggtcaaaag aactcaacaa tcccttctac agccaagcta cagcagattt tctttcaaaa
85921 aagggacaca gttgacagag aatagagtga ggaagtggaa gctgacaatt tagtgctaac
85981 ggtattgctc gcacaatagc aaaaacacaa caagagtcag caaagggtgc tgcatgctta
86041 cagtcctcac gctaacaagt atttgtaagt accaacaact tggagaggta aggggaaaga
86101 aaagcctgcc atggtgcctt gtacaggtgg cgccaaggtc cctgtctggg cccttggaca
86161 aaaccatctc atgtaaacct taaagaggat taatcctcaa ggagcactgt aatgagacct
86221 taaaaggctg aacaacagca gcagaccacg gaggaaggca gtcatctgct cccccaagtc
86281 ttgctagcag agttacatca aagcaaacgg gcagcagata gcaggcttcc acggcaatcc
86341 cttatctcgg ctccagcgga caccactact gtgattgtct tgaactgcca cctgtcccag 86401 gcatctttga aggcaacgct aagcctctga tggatctgac ctccttgagc tgagatcctt
86461 ttatgtcttt cttcattttc tgccctgcga cattcttccc ctgccatgca cacgacatga
86521 aacaaaggga gttctcactc ctgactcact gtccccagcc atttgagagc aaagaaaaac
86581 aatgttcagg attatactct gcccataata cagagctgtc ctcaggagac aagcctctta
86641 tttttgaggg tataactata gacggcattt acgtgcaaac tctgtacaat ggggttcgat
86701 ggagctgggc gccaagatag gaaagctgca tgtctgagat aactcaaaaa cacaaggaat
86761 gatcatacag aactcgatta ataaaatcac atattttcac aggaggaata caaatataaa
86821 tactgaaata ttaaaactta cttacaaaat cactgcacat tggggcttga aaaaacagca
86881 gcacacaaat atccttaaac ctggttcaac ttaaaaggaa tttttttaaa aaaatgagag
86941 agaaaaatat ttaaaacaca catatactgc aggtgagaaa agtttgaaac ctgaatggta
87001 aaacagttcc ctctgagacc tttctaagag gctagaggta ctgacttttc caaatcatac
87061 caggagaaag ggtagtgcct gtgWttctct gaaaagttct gacatttgag gagaaggaaa
87121 actgctgtga tcaaattcat tcaacacaag acttctcaac atccacttat tctgtcacac
87181 aagtctgtct gcacacattg ctttaacaac gctcacctat atgactattt ctcatactga
87241 gtgaaattgt aacatgcctc agcaagtgat ctagagtggt atgattttaa tactttgcct
87301 catttcatta aagatttcag ccagcataca agaattcaca aggtagaaaa tgctaactaa
87361 agaaaaatct gatcagagaa cagtttagat gatcaagacc tagagaaagt taagagagaa
87421 tatgcaggcc gcaatccaat agagttggaa taactgagca gctaataatc tgagtttcct
87481 ggaaacaaca atctgagttt cctggtaagc aatgagtaaa ggaaaccaaa atcagccata
87541 tgatatatcc tgcaagaagt cacataacoa cactatctta tatcatggtc ttgtgtttta
87601 tctgcacagt atttaaaatt ttcagaaaag tatacacctc acttgacata gaccagcaat
87661 tctatagcta ccatgacaac attcttctac tctgggcttt ggtctgggtt ccaaaaccca
87721 Mttattagaa gagggtcagc ctaaagggct gggaagctgt ggccttcctg tttctggatt
87781 acagggctgt ctccagccca aaagagtgag aaccccagag aaaaacccaa tgacgagcgt
87841 cagtccacag ccagccSctg aaccatctac actttacact cccacactcg atatggcctc
87901 ctcaactctg gggctattcc agctctttca gtttggattt ttttagcttt cagtttttca
87961 ccagctttca accacacagt cttgttctct gaatgacagc ctatcttccc acttctgaca
88021 tggctcttga ttgcaggcac tccttctggt aacaagcctt tttaacttcg gccagagagg
88081 cccagcagta agcggacctc agagctgatc ccaggcttgc agtagacaaa ctgcctcatg
88141 ctcttcta'tc caagctggga taaagatgtt tttctctgac accacagccc attacacatt
88201 tttcagagat gcatgatttg aaaaagaaac acactgacat gtacttcatg tgaggtgggc
88261 agcatgtatt tgaagctcat aaatctaccc acttccctct tctcaatgag atctgacttg
88321 caaaactgtt tgcagactgt ggtgccttta aacagcacac atgcatcccc atgattcact
88381 atgcccattt cttagacagg gccagtttct atgagggaat agcctatttc aatacagtgc
88441 caacttaaaa tatttaaact ccagtagtgt ttgggacaga ctgaaaataa cttttccaat
88501 tttaaagcaa cctaagtttt acagcgtatt tttatttctc caagttcatg atttttaaac
88561 aatgcagtag gcattaaaat gaaccatgtt aagaaacaaa tgtagggcct ggtgcagtgg
88621 ctgtcgcctg taatcccagc actttgggag gccaaggcag cagattgctt gagctcaggg
88681 gttagagacc agcctgggca acatggcaaa actctatctc tataaaaaaa atacaaaatt
88741 attagccagg tgtggtggca catgcctgtg gtcccagcta ctcaggaggc tgaggcagga
88801 ggattgcttg aacccaggag gcaggaggcg gaagttgcag tgagccatga tcgagccacc
88861 acactccagc ctgggcgaca gagcaagacc ctgactcaaa aaaaaaaaaa agaaaaagaa
88921 aaaaggaagg aaacaaattt aaaacagggt gccaatcaag tatcttcaaa tagacttaat
88981 atgcaataaa aaaataaaat caaagaaaat atacatatat aggaagatgt ttggagaaaa
89041 acttacctga gtagccagtc tgtaataagg gagcactcaa tttaagacac agtttgacag
89101 catcacccac tttaattgtc tgttatggta ttagccagaa ttcaattatt tgatttaaat
89161 ttctatcaac ctatcatgta tttctattga atactaccag ttggatatgg ttgttactgt
89221 ttttaatcca tttggttaaa aaaaaataaa aagtgacatt tttctcagga aatgaaagac
89281 tagctcttcc agtagcaaga agtaagattt tcaaaagact ttttggcttt tggatcactg
89341 tctttggaaa gctgcctctt taatctttgt aagtaattct ataaaatatg atacatgcct
89401 agtttttagg tagaagttta gaattaaatt catttatttt caggcttctc aaatttgtaa
89461 aatcataagg agaataaaat acatcttaaa aactaaaagt aagaaagtta taaagaagga
89521 ttcagcagca tcagaagaga tcttaaatgt gcagagggaa aaactgtcag tcagacagcc
89581 catgagccgt ccttggtatg tcagtatttt ttttttaggg tgagaaccaa gacatcagag
896411 aggtgaKggt aggaatggaa gtccatgggt cttaacaaat tatctccctt aattcctgtt
89701 atcaccctgt gcggctgcca tttccactcc cattttagag atgaggaagc cagtccagta
89761 tctaagaact tgctcaaggt cacactgcca gaaggtggca gaggtgggcc tgggtcttaa
89821 gtttaactga cactattgca gtctagcaga aatgcaacat atttaatcca tcatttccag
89881 ttcaagatag caggctgggc aaacatattt ctcacctgtc cctccaaaga cttctgtgaa
89941 atgaaaaact ggaagtaagt tttgtagtaa tgataaaata acagtaccaa agaataaaat
90001 ttaccaggcc cttactctgt gctaggcact tctaagagct ttatttatat taagtaattY
90061 aatcctcaaa acgaacccat gatgtcagag ctactcccgg ccccactgta taKatgagaa
90121 aatgggggct cagaaaggct aagtaatttg cccataatca cattgttagg agtggcagaa
90181 ttaggatttg aactgaggca gtctgggtct atggStgaaa ctcttaacac agtcaaagaa
90241 atacataaaa ttaagataaa aaatttcaac ccatttctgc aagatgcata gtgggtaaga
90301 ctggaattat aggtggacat ggtgcgtaga gaagcagcag cctgaaatat tcttcaagag 90361 ggcaaagaaa caggtggcag ctgattcacc taagaagcca tgggctgggt tgggcgcggt
90421 ggctcacgcc tgtaatccca gcactttggg aggccgaggc ggggggatca cgaggtcagg
90481 agtacaagac cagcctggcc aagatggtga aaccccgtct ctaccaaaaa tacaaaaaaa
90541 ttagctgggt gaggtggcgg gcgcctgtaa tctcagccac tagggaggct gaggcagaga
90601 actgcttgaa cccgggaggc ggagcttgca ttgagccgag atcgcgccac tgcactccag
90661 cctgggcgac agaScgagac tccatctaaa aaacaaaaaa gaaaaaagcc ctgggctggg
90721 cagatctttt accggacaca cccgtgacag tcagatggcc tgtctcctgc ccacagcaca
90781 tgagcacagg tagctggcat ttctccagat aaaaatttta tgactctttt tcaaaaaaca
90841 gaatgaactt tcctgggaaa gtgaaggctt cagcgtggat tttgaagccc tgtttccacc
90901 caggtctatc acacaaatgc actcaccaca agagtcacac cagctctaac ttctagtgtt
90961 ggtcccagca agttggtgtg acacccttat tggtacccaa gccataccgg ttcttatagc
91021 tccccagaat caagccctct tcgttctggc atgtggagaa gctccaccct atttccctaa
91081 aggaaatcct gccaactgat acacgtggtc aggatgttcc atccatccat ccaagtttcc
91141 atatgccaag cagaacaaca ttaggccagc tatccttctc cacggttttg gggaatgaca
91201 cccaaggatc actaggtttt atgaggagaa aaaaggacag gaatgagaat gaccaatatc
91261 aacaagaaaa actaaccttg gaagaaactt ttagaaggtt ttagaaagca ttacatacaa
91321 aaggcaagag caagatgtta agaagaagtt tcaataaaag atttcttaaa gttcttagaa
91381 attaagatta tgactgcaaa aagttaaaaa taatgtggag caatgaaaaa tgaagtcaag
91441 gaaatttccc agagcacaga accaaaagca aagacataca ggatatcaga gaaaagagaa
91501 gagacacaca gaagtaacct aggaggtcta acagtcaact atgtagaatt ttgggaagaa
91561 agaaaagaga aaacacagca gataaaatta ttaaagatag aatagaaatc atccatagag
91621 ttaacgaatg aatcttcaga ttcaaatggt ccatgaaggg atgggcagaa ttaatgaaaa
91681 taaaaattaa attaaaacac atctaggtac atactttcaa accactaagg ataaaaagat
91741 cccaaaagtt tccaggaagg aaaaaMataa gccatctaca aaggaataca aatcaaagag
91801 acatcagact tctcatctac aactttggat ggtaatagaa aatgtagtga tgccttaaaa
91861 gatcttgtag ggaagccttt taagttagaa ttctaactac accatcagga aagagcaagg
91921 gcagaataaa gagaatttca cagacacaaa gactcagaaa ttctgcctcc actgttgttg
91981 ggaagttact tgatgtattc caataaaata aaggaataaa ttaggaatgg caaagttgaa
92041 gtcgtaagac ctaggaaaca gtggttccaa tccagaagat cacaaaggga tgtctagaga
92101 ttcctcgttc agacttctca ggagaatgaa ttggccagga aagaagggag ctttccctta
92161 agtgcaaaag atatggttgg gaggttgggg agtgggggga tgtaagacta ttgtaaagac
92221 aaataatagg gaaaaaacta aatcaactac caaaaagcaa catgataggt aggaaagcaa
92281 ttaaagagta agtgtagata aagcacttgt tcgtaatgct Rgacacatcg ccattgggtg
92341 atttaagaat ttagaaactg gactaacaca gaaggttagg tatgatctta acattctact
92401 ggttcaacac agagaatttc cacaaccata atgctgtaga tgctttctag atcttcaact
92461 ttaaaaatca atctaYggac aaaaacatag cagatgtggt tatgatagta agaccccaca
92521 aacatattaa caaccctgac actataatag aaaagctggc tccagaagat gagatgtgga
92581 tggcacaggg gacctaacat cttactcgtt ttgctatgaa aagctgatga ggaaaagaaa
92641 gagaagctta caacactctt taaagttata aaagcaccca atatacaagt aataatataa
92701 tatcaatatt gaaagaagag ggcagagtat taaaaattaa agattaactt tctcatttca
92761 aagtcaatat attgcataag aaatatctag agttgacatg tcaggatata aaaatataag
92821 cacattctgg agttgtaatg attgtttacc aaaagaacta aaacaaaaac agtgacaagt
92881 gcccctggga ctagggttgg agaaggaagg atggaccact gtggctttcc attccagggt
92941 cttcaggact tcattattta tttatttatt tatttattca tttatttatt tatttttgag
93001 atggaggctc gctctgtcgc ccaggctgga gtgcagtggt tcaatctcgg ctcactgcaa
93061 gctccgcctc ctgggttcac gccattctcc tgcctcagcc tcccgagtag ctgggactac
93121 aggcgcccgc cactacgccc ggctaatttt ttgtattttt agtagagatg gggtttcacc
93181 atgttagcca ggatggtctc gatctcctga cctcctgatc cgcccgcctt gacctcccaa
93241 agtgctggga ttacaggcgt gagccaccgc gcccggccgg cttcatgatt tattaaaatg
93301 agcatgtgtt aacttgtaca tgataattgt catcaaatat ggtaaaaaat gttaaattat
93361 aaagagagct tcaaggtttc tttcttgctg gatctttcct agaaaaacaa caaactaata
93421 ttggaataat caaatagtca tcgcatatag aaaaaaaaag tttggctttt tcaaatccat
93481 ttcaaggaga aaactttgct tctacctggc ttggcatccc aattttggaa tattactctg
93541 aggaagaatt ctagtaacat ctctttataa cctctcagtg gattacaggc ttacagcctc
93601 acaattgtca ttcaattctg ggatcctcag aaaattagcc ccaattgttc aaccaaatct
93661 gacaccactg atcagaaatg agccaacatg agcaggttga ttttgtaaag catttagcag
93721 agcatcatct taggaaacat taaactgaga ctggaaaaaa gaccctaaaa ggtatttaaa
93781 cataaaatga gctcaaaaaa aaaaacacaa gtaatcttca cctaccaccc tatcaataat
93841 catttgcttc aaatctttgc acatcaccat attccattgt tactaacacc ataatcctct
93901 gcaccaaaac cctctcctac aatttcagaa caccaaaagg ccttcaatat taattcctaa
93961 aagaaatact tttttttaga aattaacttt gaagaatgga atttgtttaa taaaagtaaa
94021 acttatacaa aaacattctc tcatatcatc agcttactta agtgaaaaga aaacaaactt
94081 atgactcctt agaacaactc tgtttctact ttggctcttg gcaaaactag gttggcattc
94141 aaatgaaaca aggaactctg aaataaatca gtattttacc ttggtctcag atatcattag
94201 ctaaatgagg acaagtaaac gacagaacac actcaagcat tagtgttgcc gatgcctcca
94261 agttcattta cttccaaaac ccagacaatg cagcctgtgg agcttctcaa ccagtcagac 94321 tccaggcctc actcctaatt gcaccgagtt ccctccaaac actgggatca ggaaattcac 94381 agaacaggag ggctgacact acatcatYac cccactgaat cctgactctt ttcaggagtg 94441 gggccaatca cagctttcct atcaagctct gcataataga gtttctaatt agaatcttaa 94501 tactctggat agaaagcagc agaaaaaaaa aaaaaaactg tgccttagag tcttgctcac 94561 ctggtggctc attatgagag aaacacttca gaaaatctga agattaatag caggagtgtg 94621 gcatcaaagg aacaagactg caccattacc aaaaggaaaa aaaaattaag aacatcctag 94681 ctgggcgtgg tggctcctgc ctataatcct agcactttgg gaggcccagg caggcagatc 94741 acctgaggtc aggagttcga gactagcctg agcaatatgg tgaaacaccg tatctactat 94801 aaatacaaaa attagccggc gtggtgtcgc gtgcccataa tcccatctac ttgggaggct 94861 gaggcaaaag aattggttga acttgggagg tggaggttgc agtgagccga gattgtgcca 94921 ctgcactcca gcctgggcaa cagagcgaga ctctgtctca aaaaaaaaaa aaaagaaaaa 94981 aaaatcctat tggcatacat ttccaaaaag gaactctaag gtgggtctca aaagtctttt 95041 gtcctggcag tggggtaggt agtccccagg gatgacatct caagtaggct atttcacaca 95101 cagtccagca gtctgatgtc tgcgctccca gacataagaa tacatggtca ccaatgcgaa 95161 atattaactt caaataacta tgcatgagaa ggactcgtgt atgagaaaca tctagcgtta 95221 acgaccaaaa gctgaacgat tcatgtccta gaatcagttc cctgttacta taaacccatg 95281 agaagacaca gaagaaagga aaagggcact ttccgtagcc gctatggtaa acaaaacatg 95341 ccaaatgcaa cttttaacta ccctagctca gagggcaaac ccacaaaaac ccagaaaatt 95401 tattacttct gagtttatta gattttgggg acaggaacta tgtgctaaat taggcatgct 95461 gtgggctctc aggtttgata aatcaggtat gaggcagaat gaaatcaaca ctgacaaaga 95521 ctagctgcgc accaccctca gaatatgaca gcacgtgcca acatcactga agggccttga 95581 aaggtcatta gacaataaac tatgtgcttg gctgaaatat tagcaagggt caggatgagc 95641 tcacgtgaaa ttttccctgc aagaaaactc taaaattcag gggagcattt cagattgcag 95701 ttcacaactc ctcataaatt aatgaaagtc aggcattaaa cttaataaaa gctggaacgg 95761 atcaagcaga aagaaaacgc agcatcggca tatgtgttat gtgaatttct cagagtccat 95821 aaatttgata tttggaagga agaagggaaa tgttaattag gagaatttca tagtgKattt 95881 aaatttgcca tggagtcaaa gctctattgt aaaaaccccc ttggaatctg aaatcaccgt 95941 caccagatct aggtcctgcc ttttatcttg ctattttaca cttgttgata tgcaacttaa 96001 ttgtttcatg tgttgtttct tattttctct ccccaaatta taaaatctag aacgctaagg 96061 tcatgtcttt tattctactg tgtgtggagc tcaattaatg ttggggaacc agtaaaaaaR 96121 tctaaaaatt catccctctg acttctctaa aagccttgta gcaatttttc atgatcttta 96181 gactattcta gctagctata tctcagagta ttatagtcac tcttaaaagt ctcaatgttg 96241 ccttttctta ttttcctgat gtagcaggag agcattgcca caacatgtat ctttatctca 96301 cagaccagat atatctcagg tcaaatcaag tcccctgaaa cattacttta tacagatcca 96361 ggcagatatc tcatatttgt ctttaaagca agtatcagtc ctacttgcag gtcccagaga 96421 caggaaggga tcccagtggt cttcttctta ctgttttcac agattttcta ctcagagctc 96481 tctgtgtcag tcttggcctt ccttagcata tatttcttta agttattggt tgagaatttc 96541 catgtctgtc tttagcctgc acgggccctg ctatctatac tcagtccttt tgatcttttc 96601 tcataaatca ttcctccaac ctcttcatca tgcttaagat tcttttagtt tatctgagac 96661 accaaagtca ggagacatga actgtccata ttagcgggac tgttggatac caagatgcct 96721 ttgtggaaaa aaactgtttt ctctctgtct ctctctctca ctttttttct ctttctctcc 96781 ccctgccaca tgtaagcatt aagtatattt ataaacaaag gggatactgt cttcccacca 96841 cctcctcccc tgggtggggc agggtggttt gcagccctgt gtagaaccac atatagagac 96901 agttaaggat cttacagtgg actctgtatg gctggtagag ctggtcccca aaaatgccta 96961 ggtagggcca gatactggct tgtattatct gttgacatgt gaaagctcca gttattatac 97021 aaactgttct tgagaactga gatttcacat aatgaattaa gatctttgat ccaaagagac 97081 agcccatctt ccaaaagcca ctgcaccatt aacgaagcag atgtgggggc aacaggagca 97141 ccatgagaag gacaacgttt tataagacag ctagaaaagg aatgcacgta ggcctccgtt 97201 tctcagttac aaagccgagg tctggagtcc agactcaaac cagcaccatg agcaggctgt 97261 ataatctcca taattataat tcaagtgcta aatgagtttg tgtgaaaact aaagaagatc 97321 acataatgag gtcctcagcc cagtgcttgg cacatgggaa atatcacaca tatggcctgt 97381 ggtggtgatt actactgtgt accaactctt acatcctcca aaacatgggg gtacagagag 97441 gaccacaggc ttccccattt ttaacttcta ggtaaggtaa tgttaagttc tttgcaaaat 97501 atcagttaac aaaataagaa gaaatgtctc attctcccct acaacccaac acctggagca 97561 cRgggagggc agaggtagca aggaccagtt cctgcctctc tgacactggg atgcataaga 97621 acaaagcctg gacaccgaag tggcatttgg cctgccttcc ctgattctac acccttgtac 97681 tccaagcccc tgcccaaaac tggcaatgcc accacctggg ttccaggaca tagtttccaa 97741 gcactcccca gctgggctga tcccaaacca ctggcaacct agccacgctc ttgcacagat 97801 ctggactatc attttgaaat ctcatccgtt atgggacatg ggtgaagaat ctgagttctg 97861 gagccaataa accgggttca aacctcagtt cagccacttc caaaccacgt aaaaatggac 97921 aacttactta gctttaccag gtctcagttt cctcatatgt caaaggggaa aatcatgaca 97981 accgctcata aggttgtttt gtggggattt agtgacttca agagcaaagc ccatggcata 98041 acattgtggt gcagacagag agcttaMttt ctgctggcta tcatttcagc aaggatcatt 98101 tcccacctgg gccactgcaa cagcagcctg ctaagtcacc tccctgattc cacattccat 98161 gccttcacaa acgtcactgc acaccagcca gagtgacagc cctaaaataa aaatcaaatc 98221 acaacaYttc ttccactggc tttttgttgg cccacaaggt ccctcaatct catctccccc 98281 tactgtccca Mcccctggcc aatgaccaca ttggcatgct ctccgctcct ggtacttttt
98341 cacggcaaac ctttcagtag aatgctctcc ctcagatctc ccagggcagc ttctcttttt
98401 ttattcagtt attctggctg caaagacacc tcctgagcaa ggctttccct gccccaccca
98461 cctgacctaa ggcacaacat tectacccaa gtctctctct agcacatcac ctggtttatt
98521 accctcagag caccaccagc tataggttga gtatccctaa tctgaaaatc tgaaatgctc
98581 caaaatccaa aactctgaga gctgagatga tgccacaggt gaaaaattcc acacctgacc
98641 gacctcatgt gatgggtcat agtcaaaatg cagctaaaat tgtttcatgc ataaaattgt
98701 tttaaaatac tacataaaat cgccttaaga ctgtgtggaa aaggtatata taaaacataa
98761 atgaattgtt tgtgtagact tggatttcat ctccaagata tctcattcta tatatgcaaa
98821 tattcagaat ctgaaatcta aaacacttct agtcctaagc atttcagata aggggtactc
98881 aacctgtatc ttcttgttaa ggctctgcct aggtgtttgt tgtatgtctc ttctggctgg
98941 tatgtcagct ttgagaacaa gaacctgatt tgttttggca aacgctgacc cctcaggacc
99001 tagaatagtg tctgactcac agcaggctct caaaacactg cacaaagttt tttaatgaaa
99061 ataaatatca tgcatcatgt acttaatgga gtgagactcg aggcaggtca cgtcagcagt
99121 gttatcagaa ggactgagtc ccccagaggc actcaggtgt ctccaattgt tcactatctc
99181 ccacatcatc gatctgtggc aatgtgataa acacatactg catttaaaac atcattcatc
99241 ataaaagata gaggctacat aaaaatgcat ctcacagacc tatatatgag aggtaaactg
99301 cacatttgat caaaaggtca acccacttgg tcaggttcac tgagccaagc tgggatcaca
99361 gacaccaagt tggagtggat cacagattct ctagcaggga gcgaggtcac aggaaagcta
99421 ccattgtatg ttagtttccc ttcacccNtg ggggaggagg gaataaattt cttacaaaga
99481 ggagacacag gggtagggga agggaaagga aatgtgaaat cttgccccat ttgaaaacag
99541 gcttattcca cgaaagcaaa ccagaagaag agctggcatt tgcttctcag catcccccac
99601 gtgcacttct ctgctcttgt ctaaattacc taggaaacag gcacctcctt
[0384] Following is a first SLC22A1 coding nucleotide sequence (cDNA, SEQ ID NO: 10). NM_003057 - SLC22A1 (Isoform a) ctgatttcaaaccactccttttcaaagatctctgagggagacattgcacctggccactgcagcccagagcaggtctggccacggccatgagcat gctgagccatcatgcccaccgtggatgacattctggagcaggttggggagtctggctggttccagaagcaagccttcctcatcttatgcctgctgt cggctgcctttgcgcccatctgtgtgggcatcgtcttcctgggtttcacacctgaccaccactgccagagtcctggggtggctgagctgagccag cgctgtggctggagccctgcggaggagctgaactatacagtgccaggcctggggcccgcgggcgaggccttccttggccagtgcaggcgct atgaagtggactggaaccagagcgccctcagctgtgtagaccccctggctagcctggccaccaacaggagccacctgccgctgggtccctgc caggatggctgggtgtatgacacgcccggctcttccatcgtcactgagttcaacctggtgtgtgctgactcctggaagctggacctctttcagtcct gtttgaatgcgggcttcttgtttggctctctcggtgttggctactttgcagacaggtttggccgtaagctgtgtctcctgggaactgtgctggtcaacg cggtgtcgggcgtgctcatggccttctcgcccaactacatgtccatgctgctcttccgcctgctgcagggcctggtcagcaagggcaactggat ggctggctacaccctaatcacagaatttgttggctcgggctccagaagaacggtggcgatcatgtaccagatggccttcacggtggggctggtg gcgcttaccgggctggcctacgccctgcctcactggcgctggctgcagctggcagtctccctgcccaccttcctcttcctgctctactactggtgt gtgccggagtcccctcggtggctgttatcacaaaaaagaaacactgaagcaataaagataatggaccacatcgctcaaaagaatgggaagttg cctcctgctgatttaaagatgctttccctcgaagaggatgtcaccgaaaagctgagcccttcatttgcagacctgttccgcacgccgcgcctgagg aagcgcaccttcatcctgatgtacctgtggttcacggactctgtgctctatcaggggctcatcctgcacatgggcgccaccagcgggaacctcta cctggatttcctttactccgctctggtcgaaatcccgggggccttcatagccctcatcaccattgaccgcgtgggccgcatctaccccatggccat gtcaaatttgttggcgggggcagcctgcctcgtcatgatttttatctcacctgacctgcactggttaaacatcataatcatgtgtgttggccgaatgg gaatcaccattgcaatacaaatgatctgcctggtgaatgctgagctgtaccccacattcgtcaggaacctcggagtgatggtgtgttcctccctgtg tgacataggtgggataatcacccccttcatagtcttcaggctgagggaggtctggcaagccttgcccctcattttgtttgcggtgttgggcctgctt gccgcgggagtgacgctacttcttccagagaccaagggggtcgctttgccagagaccatgaaggacgccgagaaccttgggagaaaagcaa agcccaaagaaaacacgatttaccttaaggtccaaacctcagaaccctcgggcacctgagagagatgttttgcggcgatgtcgtgttggaggga tgaagatggagttatcctctgcagaaattcctagacgccttcacttctctgtattcttcctcatacttgcctacccccaaattaatatcagtcctaaaga aaaaaaaaaaaaaaaa
[0385] Following is a second SLC22AΪ cDNA sequence (SEQ ID NO: 11). SLC22A1 (Isoform b) ctgatttcaaaccactccttttcaaagatctctgagggagacattgcacctggccactgcagcccagagcaggtctggccacggccatgagcat gctgagccatcatgcccaccgtggatgacattctggagcaggttggggagtctggctggttccagaagcaagccttcctcatcttatgcctgctgt cggctgcctttgcgcccatctgtgtgggcatcgtcttcctgggtttcacacctgaccaccactgccagagtcctggggtggctgagctgagccag cgctgtggctggagccctgcggaggagctgaactatacagtgccaggcctggggcccgcgggcgaggccttccttggccagtgcaggcgct atgaagtggactggaaccagagcgccctcagctgtgtagaccccctggctagcctggccaccaacaggagccacctgccgctgggtccctgc caggatggctgggtgtatgacacgcccggctcttccatcgtcactgagttcaacctggtgtgtgctgactcctggaagctggacctctttcagtcct gtttgaatgcgggcttcttgtttggctctctcggtgttggctactttgcagacaggtttggccgtaagctgtgtctcctgggaactgtgctggtcaacg cggtgtcgggcgtgctcatggccttctcgcccaactacatgtccatgctgctcttccgcctgctgcagggcctggtcagcaagggcaactggat ggctggctacaccctaatcacagaatttgttggctcgggctccagaagaacggtggcgatcatgtaccagatggccttcacggtggggctggtg gcgcttaccgggctggcctacgccctgcctcactggcgctggctgcagctggcagtctccctgcccaccttcctcttcctgctctactactggtgt gtgccggagtcccctcggtggctgttatcacaaaaaagaaacactgaagcaataaagataatggaccacatcgctcaaaagaatgggaagttg cctcctgctgatttaaagatgctttccctcgaagaggatgtcaccgaaaagctgagcccttcatttgcagacctgttccgcacgccgcgcctgagg aagcgcaccttcatcctgatgtacctgtggttcacggactctgtgctctatcaggggctcatcctgcacatgggcgccaccagcgggaacctcta cctggatttcctttactccgctctggtcgaaatcccgggggccttcatagccctcatcaccattgaccgcgtgggccgcatctaccccatggccat gtcaaatttgttggcgggggcagcctgcctcgtcatgatttttatctcacctgacctgcactggttaaacatcataatcatgtgtgttggccgaatgg gaatcaccattgcaatacaaatgatctgcctggtgaatgctgagctgtaccccacattcgtcagcggtgttgggcctgcttgccgcgggagtgac gctacttcttccagagaccaagggggtcgctttgccagagaccatgaaggacgccgagaaccttgggagaaaagcaaagcccaaagaaaac acgatttaccttaaggtccaaacctcagaaccctcgggcacctgagagagatgttttgcggcgatgtcgtgttggagggatgaagatggagttat cctctgcagaaattcctagacgccttcacttctctgtattcttcctcatacttgcctacccccaaattaatatcagtcctaaagaaaaaaaaaaaaaaa aa
[0386] Following is a TTNcDNA sequence (SEQ IDNO: 12). Other transcript variants are described and each is incorporated herein by reference in its entirety. NM_133378 - TITIN (isoform N2A) agcagtcgtgcattcccagcctcgcctcgggtgtagggattgcatagaaaagcaaaactacacagtcttgactgtgtaglLUgUUlaggattaga ggctcaccgattcatgtcggagatggtcagaaaaaccaactctccataggacgtcgtttcagaagcaaccttgggcttagtcccaccctttttagg cactcttgagaaatcaagtgcctagaaagatgacaactcaagcaccgacgtttacgcagccgttacaaagcgttgtggtactggagggtagtac cgcaacctJlgaggctcacattagtggttttccagttcctgaggtgagctggtttagggatggccaggtgatttccacttccactctgcccggcgtg cagatctcctttagcgatggccgcgctaaactgacgatccccgccgtgactaaagccaacagtggacgatattccctgaaagccaccaatggat ctggacaagcgactagtactgctgagcttctcgtgaaagctgagacagcaccacccaacttcgttcaacgactgcagagcatgaccgtgagac aaggaagccaagtgagactccaagtgagagtgactggaatccctacacctgtggtgaagttctaccgggatggagccgaaatccagagctcc cttgatttccaaatttcacaagaaggcgacctctacagcttactgattgcagaagcataccctgaggactcagggacctattcagtaaatgccacc aatagcgttggaagagctacttcgactgctgaattactggttcaaggtgaagaagaagtacctgctaaaaagacaaagacaattgtttcgactgct cagatctcagaatcaagacaaacccgaattgaaaagaagattgaagcccactttgatgccagatcaattgcaacagttgagatggtcatagatgg tgccgctgggcaacagctgccacataaaacacctcccaggattcctccgaagccaaagtcaagatccccaacaccaccgtctattgctgccaa agcacagctggctcggcagcagtccccatcgcccataagacactccccttccccggtcagacacgtgcgggcaccgaccccatctccggtca ggtccgtgtctccagcagcaagaatctccacatcccccatcaggtctgttaggtctccattgctcatgcgtaagactcaggcatccaccgtggcc acaggtcctgaagtgcctcccccttggaagcaagagggctacgtggcctcctcatctgaggctgagatgagagagacaacgctgacaacctct actcagatcaggacagaagagagatgggaagggagatacggtgtccaggagcaagtgaccatcagtggtgctgcgggtgctgccgccagtg tgtcggccagtgctagctacgcagcagaggctgttgccactggtgctaaagaggtgaaacaagatgctgacaaaagtgcagctgttgcgactgt tgttgctgccgttgatatggccagagtgagagaaccagtgatcagcgctgtagagcagactgctcagaggacaaccacgactgctgtgcacat ccaacctgctcaagaacaggtaagaaaggaagcggagaagactgctgtaactaaggtagtagtggccgccgataaagccaaggaacaagaa ttaaaatcaagaaccaaagaagtaattaccacaaagcaagagcagatgcacgtaactcatgagcagataagaaaagaaactgaaaaaacattt gtaccaaaggtagtaatttccgcagctaaagccaaagaacaagaaactagaatttctgaagaaattactaagaaacagaaacaagtaactcaag aagcaataagacaggaaactgagataactgctgcatccatggtggtagttgccactgcaaagtccacaaaactagaaacagtcccgggagctc aagaagaaactaccacacaacaagatcaaatgcacctaagttatgaaaagataatgaaggaaactaggaaaacagttgtacctaaagtcatagtt gccacacccaaagtcaaagaacaagatttagtatcaagaggtagagaaggcattactaccaaaagagaacaagtgcaaataactcaggagaa gatgagaaaggaagccgagaaaactgccttgtctacaatagcagttgctactgctaaagccaaagaacaagaaacaatactgagaactagaga aactatggctactagacaagaacaaatccaagttacccatggaaaggtggacgttggaaaaaaggctgaagctgtagcaacagttgttgctgca gtagaccaggcccgagtcagagagcccagagagcctgggcatcttgaagaatcctatgctcagcagaccactttggagtacggatataagga acgcatttccgccgcaaaggtagctgagcctccccaacgtccagcctcagaaccccacgttgtccctaaagcagtcaagcctagagtaatcca ggctccttctgagactcatatcaaaactactgatcaaaagggaatgcacatatcatcacagatcaagaaaactacagatctaacaacggaaagat tagtccatgtggataaacgcccccgcacagctagccctcactttactgtttcaaaaatttctgttcctaagacagaacatggatatgaggcatcaat agccggtagtgctattgccacattacaaaaagagttgtcagccacatcttctgctcagaagatcaccaaatcggtgaaggctcctactgtgaagc ccagtgagactagagtaagggcagagcccacacccttgccacagttccccttcgctgacacaccagatacttacaagagtgaagctggcgttg aggtgaaaaaggaagtaggggtgagcatcactggcaccaccgtccgtgaagagcgctttgaagtactgcacggacgcgaagccaaggtaac agaaacagcaagagtaccagcacctgttgaaattcctgttactccaccaactttggtctcgggcttaaaaaatgtgactgtcatagaaggtgaatct gtcaccttggagtgccacatctctggatacccatccccgacagtgacatggtacagggaagactaccaaatcgaaagttccattgacttccagat aaccttccagagtggaattgctcgtcttatgattcgcgaagcatttgcggaagacagcgggcgatttacttgcagtgctgtaaatgaggctggaac cgtcagcacatcctgctatctggctgtgcaggtgtcagaagaatttgaaaaggaaaccacagccgtgactgagaaatttactacagaagagaaa cgctttgttgagtcaagagatgtggttatgactgatactagcctcacagaggaacaagcagggcctggagaacctgccgcgccttactttattaca aaaccagtggtccagaaactggtggaaggtgggagcgtggtgtttggatgccaagttggcggcaacccaaagccccatgtatactggaaaaa atctggtgttcctctaaccactggatacagatacaaagtgagttacaacaaacaaaccggtgaatgcaagctggtgatttctatgacttttgctgatg atgctggagaatacactattgttgttcgcaataagcatggagaaacttctgcatctgcttccttgcttgaagaagctgattatgagttactgatgaagt cccagcaagaaatgctttatcagacacaagtgactgcatttgttcaagaacctaaagttggagaaacagcacctggatttgtatactctgagtatga aaaagagtatgaaaaagaacaagccttaattaggaagaaaatggccaaagatactgtagtggtcagaacttatgtagaagatcaggaattccata tttcttcctttgaagagagacttattaaagaaattgaatatagaataataaagactacattagaagaacttcttgaagaagatggagaagaaaagatg gcagttgacatttctgaatctgaagctgttgaatcaggatttgatttaagaatcaagaattatagaattcttgaggggatgggtgtcacttttcattgca agatgtctggatatccattaccaaagattgcttggtacaaagatggcaagcgcatcaaacatggagaaagataccaaatggactttctacaagat ggcagagctagtctgcgtatacctgttgttcttccagaagatgaaggaatctacactgcatttgccagcaatattaaaggaaatgcaatttgctcag ggaaattgtatgtggagcctgctgcaccacttggagctccgacttacattcccacactagagccagtgagcagaatcagatctctctctccacgtt cagtgagcaggtctcctatacgcatgtctcctgcacggatgtcacctgcaaggatgtctcctgcacggatgtcccctgcaagaatgtcccctgga cgtaggctggaggagacagatgagtcacaacttgagagactatataaaccagtctttgtgttaaaacctgtttctttcaaatgtttagaagggcaaa ctgccagatttgacttaaaggttgttggtagacctatgccagagacgttctggtttcatgatggccagcaaattgtcaatgactatacccataaagta gtcattaaagaagatggtactcaatcactaattattgtccctgccacacccagtgattctggggaatggactgtggttgcccaaaacagggcagg cagatcttcaatttcagtgattttaactgtggaagctgtggaacatcaggtaaaaccgatgtttgtagaaaaactgaaaaatgtcaatataaaggaa ggttcccgacttgaaatgaaagtcagagctacgggtaaccccaaccctgacattgtatggttgaaaaacagtgacatcattgtgcctcataaatat cccaaaatcagaattgaaggaaccaagggagaagctgcccttaaaatcgattccactgtcagccaagattctgcctggtatactgcgactgctatt aataaagctggcagagacactacaagatgcaaagtaaatgttgaagttgagtttgcagagcctgagccagagagaaagttaatcatcccacgg gggacatatagagcaaaggagattgcagccccagaactggagcccctccatttgcgatatggccaagagcaatgggaagaaggtgatctctat gacaaagagaaacaaGagaaaccatttttcaagaaaaaactcacttccttaagacttaagcgctttgggcctgcccactttgaatgcaggctaaca cccattggtgacccaacgatggtggtggagtggctccatgatggaaagccacttgaagcagccaacaggctccgtatgatcaatgaatttgggt actgcagccttgattatggcgttgcatattctagagacagtggtatcattacttgcagagccactaacaaatatggaacagatcacacatctgctac ccttattgttaaagatgagaaaagtcttgtggaagaatcccaattgcctgaggggaggaaaggcttacagagaattgaagaattagagagaatgg ctcatgaaggtgcacttacaggtgtaacaacagatcagaaagaaaagcaaaagccagacattgtcttgtacccagagccagttagagtacttga aggggagactgcaaggttccgctgcagggtaacaggctaccctcagcccaaagtcaactggtacctcaatggacagctcatccgcaaaagca aaaggttcagagttcgctatgatggtatccattacctggacatcgtggactgcaaatcatatgacacaggtgaagtgaaggtcaccgcggaaaat cctgaaggtgtgatagagcataaagtgaagcttgagattcaacagagggaagattttaggtctgtccttaggagagctcctgaaccaaggcctga gtttcacgtacatgaaccaggaaagcttcagtttgaagtacaaaaagtggatagacctgttgacaccactgaaaccaaagaagttgtgaagttga aaagggctgaaagaattacccatgaaaaagtgcctgaagagtcggaagagctgcgcagtaaattcaagcgcagaacagaagagggctattat gaagccattaccgctgtggagctcaagtctcgaaagaaggatgaatcctatgaggaactcctcaggaagacaaaagatgaacttctccactgga ccaaagagttaactgaagaggaaaagaaagctcttgccgaagaaggcaaaatcacgattccaacttttaaacctgacaagattgaactaagtcct agtatggaggctccaaaaatcttcgaaagaatccagagccaaacagtgggccaaggatctgatgcacacttccgggtcagagtcgtggggaa accagaccccgaatgtgaatggtacaaaaatggtgtcaaaattgaacggtctgaccggatctactggtactggcccgaagacaatgtttgtgaat tggtcataagagatgtgactgctgaggactctgccagcatcatggtaaaagccatcaacatagctggagaaacctccagtcacgcattcttacttg tccaagccaagcaattgatcactttcacacaggaattacaagatgttgttgctaaggaaaaagacactatggcaacctttgaatgtgaaacttcaga accatttgtcaaagtgaaatggtataaagatggtatggaggttcatgagggagataaatacaggatgcactctgacagaaaggttcacttcctctc catactgaccattgatacgtctgatgctgaagattacagctgtgtacttgtggaagatgaaaatgtcaaaacgactgctaaacttattgttgaaggtg cagttgttgagtttgtgaaagaacttcaggacatagaagttccagaatcatattcaggagaattagagtgcattgtatccccagaaaatatagaagg aaaatggtatcataatgatgtggagcttaaatccaatggcaaatatacaattacatctcgtcgtggacgtcagaacctcacggtcaaggatgtaac caaggaggaccagggagaatacagctttgtcatcgacgggaaaaagacaacctgtaaattaaagatgaaaccccgccccattgctatcctaca aggacttagtgaccaaaaagtctgtgagggtgacattgttcagcttgaagttaaagtctccttggaaagtgtggaaggcgtctggatgaaagacg gccaagaagtgcagcccagtgacagggttcacattgtgatagacaaacaatctcatatgctgctcattgaagacatgactaaggaagatgctgg aaattactctttcaccattccagcccttggcctctccaccagtgggcgtgtctctgtctatagtgtggacgtgataacacctctaaaagatgttaatgt gattgaaggcaccaaggctgtgcttgaatgtaaggtgtcagtccctgatgtgacttctgttaagtggtacttaaatgatgaacaaatcaagcctgat gaccgtgtacaggccattgtgaaaggtactaaacagcgactagtcattaaccgaactcatgcttcagacgaaggaccttacaagctgatagttgg cagagttgaaaccaactgtaatctctctgtagaaaaaattaaaattatcagaggtcttcgtgaccttacctgtacagaaactcaaaatgtggtgtttg aggttgagctgtcccactctggaattgatgtcctgtggaattttaaggacaaggaaatcaagcccagttctaaatataaaattgaagcacatggaa aaatatataaattgacagttctaaatatgatgaaagatgatgaaggaaaatacacattttacgcgggagaaaatatcacatctggaaaacttactgt ggcaggtggggccatctccaagccactcacagatcagaccgtagctgaatcccaggaagctgtgtttgaatgtgaagttgccaacccagattcc aaaggcgaatggttgagggatggcaaacacctaccactgactaacaacatcagaagtgagtctgatggccacaaaaggagacttatcattgctg ccaccaaattagatgacattggagaatatacctacaaggtggccacctccaaaacatctgccaaactcaaagttgaagctgtcaaaattaagaag actctgaagaacctcacagtgacagaaacacaggatgctgttttcactgtcgagcttacacaccctaatgtcaaaggtgtccagtggatcaaaaat ggagttgtgctggaatccaatgaaaagtatgctatctctgtcaaaggaacaatttactctctgaggattaaaaactgtgccatcgtggatgagtctgt ttatggcttcaggcttggaaggcttggagccagtgccagactgcacgtggagactgtcaagatcattaaaaagccaaaggatgtgacagccttg gaaaatgccactgttgcctttgaagttagtgtttcccatgacactgttccagtaaaatggttccataagagtgtggaaattaagccaagtgacaaac acagactggtctcagaaaggaaagtccacaagctgatgctgcagaacatctccccctcagatgctggggaatacacagctgtggtcgggcaat tggaatgcaaagcaaaactgtttgtggagacattacatattacaaaaaccatgaaaaatatcgaggtgcctgagaccaaaactgcctcttttgagt gtgaggtgtcccacttcaatgtcccttccatgtggctgaagaatggtgtggaaattgagatgagtgaaaagttcaagatagttgtgcagggaaaac tccatcagctgatcatcatgaacaccagcacagaggactcggcagaatacacatttgtctgtggcaatgaccaagtcagtgccaccctgacagt caccccaatcatgattacttccatgctgaaagacatcaacgctgaagaaaaagacactattacttttgaggtgacagtgaactatgaaggcatctct tacaaatggttaaagaatggtgtggaaatcaaatcaactgacaagtgccagatgagaaccaaaaagctcacacactcactgaacatcaggaatg ttcactttggggatgctgctgactacacctttgtggctggaaaagcaacatcaacagccacactttatgtggaagctcgtcatatagaatttaggaa acacattaaggacattaaggtactggagaagaagcgagccatgtttgaatgtgaagtttctgaacctgacatcactgtacagtggatgaaagatg accaggaactgcagatcacagacagaataaagattcagaaggagaaatatgtccaccgccttctgatcccatccacccggatgtctgatgctgg gaagtacacagtggtggcaggaggcaacgtgtcaactgcaaaactctttgtagaaggcagagatgttcgoatccgaagtattaaaaaggaggtt caggtcattgagaaacagcgtgctgttgttgaatttgaggtcaatgaagacgatgttgatgcccactggtataaagatggcattgaaatcaatttcc aagttcaagaacgacacaaatatgtagtggaaagaagaatccaccgaatgtttatctctgagaccagacagagcgatgcaggagaatacacctt tgtggcaggaaggaacaggagttctgtcactctctatgtcaatgctcctgaaccgccccaagttctgcaggagctccagcctgtcactgtgcagt ctggcaagcctgcccgcttctgtgccgtgatatccggaagaccacagcccaaaatttcctggtacaaggaagagcagctgctttccactggcttc aagtgcaaatttcttcatgatgggcaagagtacacgcttttgctaattgaagccttcccagaggatgcggcagtctatacctgtgaagccaagaat gactatggtgttgccacaacatcagcttcactctcagtggaagttccagaagttgtgtctcctgatcaggaaatgcctgtttatccacctgccatcat caccccgcttcaggacactgtcacttctgaagggcagccagcccgttttcaatgccgggtttctggaacagatctaaaagtgtcgtggtacagca aagacaagaaaatcaagccatctcggttctttagaatgactcaatttgaagacacttatcaactggaaattgccgaagcttatccagaagatgaag gaacttacacgtttgttgctagtaatgctgtaggccaagtatcaagcacagccaacctgagtctggaagctcctgaatcaattttgcatgagaggat tgaacaagagattgagatggaaatgaaagagttttctagttcttttctgtctgccgaggaagaaggacttcatagcgccgaacttcaattatctaaaa taaatgaaacacttgaacttttgtctgaatctccagtttactcaactaaatttgattccgaaaaggaaggcactggcccaattttcatcaaagaagtgt caaatgctgatataagcatgggggatgtggctacactgtctgtaactgtcattggcatccccaaacctaaaattcagtggttctttaatggagtgcta ttaaccccttctgctgactacaaatttgtttttgacggtgatgatcatagcctgatcattctgttcaccaaattggaggatgagggagagtatacatgt atggccagtaatgactatggaaagacaatatgtagtgcctatctaaaaattaattccaaaggagagggtcacaaagacactgaaacagaatcag cagtggcaaaatctctggaaaagctgggaggtccttgtcctcctcacttccttaaggagttaaaaccaattcgctgtgctcaagggcttcctgccat ctttgagtacacagtggttggagagcctgcccctactgttacatggttcaaagaaaacaagcagctttgcaccagtgtttattacactatcattcata accctaatggctctggaactttcattgtcaatgaccctcagagggaagacagtggcctctatatctgtaaagcagagaatatgttgggtgagtcca cctgtgcagcagagctgcttgtgcttctggaagacacagacatgactgataccccctgcaaagcaaagtccacaccagaggctcctgaggattt tccacagacacccttaaagggtcccgcagttgaagcacttgactcagagcaggaaattgcaacgtttgtaaaagacaccattttgaaagctgcttt aattacagaagaaaaccagcaactatcttatgagcatattgctaaagccaatgaattgagcagtcagcttcctttgggagctcaggaattgcaatc cattttggagcaagacaagctcactcctgaaagcaccagggaatttctttgcatcaatggcagtattcactttcagcctctcaaggaaccatctccc aacctacagctgcagattgtacagtcccagaaaaccttctccaaagaaggtattctaatgcctgaagagcctgagacacaggcagttctatcaga taccgagaaaatcttcccaagtgccatgtccatagaacaaattaattcattaacagttgagcctctgaaaactttattagctgaacctgaagggaatt atccacagtcttcaatagaacctccaatgcattcttatctaacctctgtggctgaggaagtactttcaccaaaagaaaagacagtatctgacaccaa cagagagcaaagagtgactcttcaaaagcaagaggcacaaagtgcgctcatcttgagtcagagcttagctgagggacacgtggagagtctcc agagtcctgatgtcatgatctctcaggtaaactatgagcccctagtcccttcagaacactcatgcacagaaggaggtaaaattttgatagaaagtg caaatccactggaaaatgcagggcaagattctgcggtcagaattgaggaaggcaagtccttaagatttccactagcacttgaagaaaagcaggt actgctcaaagaagagcattctgacaacgtggtgatgcccccagaccaaatcattgagtctaaaagagagcccgtggcaataaagaaagtgca ggaggtacagggaagggaccttctttctaaggaaagcttgctttctggtattccagaagagcagagattaaacctgaaaattcaaatctgccggg ctttgcaagcagccgtggccagcgagcagccaggtcttttctctgagtggctaagaaatattgaaaaggtggaggtcgaggctgtaaacatcac ccaagagcccagacacatcatgtgcatgtaccttgttacttcggcaaagtctgtaacagaagaagtaaccatcattattgaagatgttgatcctcaa atggctaacctgaaaatggaacttagggatgctttgtgtgctattatatatgaggaaatagacatcctaacagctgagggtcctagaattcagcaag gagccaaaacaagtttgcaagaagaaatggattctttttcaggttcacagaaggttgaacccattactgaaccagaagttgaatctaaatatctgat ctcaactgaagaggtcagttattttaacgtgcaaagtagggttaaatatttggatgccacacctgtcactaaaggggttgcttcagctgttgtctctg acgaaaaacaagatgagagtctgaaaccatcagaggaaaaagaggagtcttcctctgaaagtggtactgaggaggttgctacagtaaagatac aggaagctgagggtggcttaatcaaagaggatggccccatgatacatacacctttagtggacactgtttctgaggaaggtgatattgtacacctca caacatccataacaaatgctaaagaggtgaattggtattttgagaataaactggtgccttcagatgaaaagttcaagtgtttacaagatcaaaatac atatacgctagtcatcgacaaagtaaataccgaagaccatcaaggagagtatgtctgtgaggccttgaatgacagcggaaaaacagcaacttca gccaaactcactgtagtaaaaagagctgccccagtgatcaagaggaaaatcgaacccctggaagtagcactgggccacctagccaaattcacc tgtgagatccaaagtgctcccaatgtccggttccagtggtttaaagctggccgagaaatttatgagagtgacaagtgttctattcgatcttcaaagta tatctccagccttgaaatcctgagaacccaggtggttgactgcggcgagtatacatgcaaagcttccaatgagtatggcagtgtcagctgtacag ccacactaactgtgacagaggcatatccaccaacctttctctccagacctaagtcccttacaacttttgtgggtaaggcagccaagttcatctgcac agtgacagggactcctgtgatagaaaccatttggcagaaagatggtgctgcactctcaccttcacctaactggaggatttccgacgcagaaaac aaacacattttagaactctcaaaccttaccattcaagatagaggagtttattcttgtaaggcttctaacaagtttggagcagacatctgccaagcaga gttgatcatcattgataagccacatttcattaaagaattagagcctgtgcagtccgctatcaataagaaggtccaccttgagtgccaagtagatgaa gacagaaaagtcacagttacgtggagcaaagatgggcaaaaactccccccagggaaagattataagatctgttttgaagataaaatagcaacac ttgagattcctttggccaaactgaaagattcaggaacctatgtctgtacagcttcaaatgaggctggaagcagctcctgctcagccacagtcactg tcagagagccaccatccttcgtgaagaaggtggatccctcttatttaatgctcccaggtgaatcagcacgcctccattgcaagctgaaaggctca cctgtgatccaggttacttggtttaaaaataacaaagaactcagtgaaagtaacacagtccgaatgtattttgtcaattctgaggctatacttgatatta cggatgtaaaagttgaagacagtgggagttactcatgtgaagcagtgaatgacgtcggcagtgatagctgcagtactgaaatagttatcaaagaa cctccttctttcatcaaaactcttgagcctgcagacatagtgagaggaacaaatgctctacttcagtgtgaagtctcaggcactggaccatttgaaat tagctggttcaaagacaagaaacaaattcgaagtag^aaaaaatacagattgttttctcagaagtctcttgtgtgtctggagatcttttcgtttaatagt gcagatgttggtgaatatgaatgtgttgttgctaatgaagtcggcaagtgtggctgcatggcaacccacttactcaaagaacctccaacctttgtaa agaaagtagatgatttgattgcactaggaggacaaaccgttaccctgcaagctgctgtgagagggtcagagcccatttctgtcacatggatgaaa ggtcaagaggtcatcagagaagacggaaaaatcaaaatgagcttttccaatggtgttgcagtcttgataatccctgatgttcagattagttttggag gcaaatacacgtgcctggctgaaaatgaagctggaagccaaacatctgttggggaattaatagttaaagaacctgccaaaatcattgagcgagc agaactgatccaggtgacagcaggagatcccgccacactggagtacacagtggcaggcacgccagaactgaagcccaaatggtacaaagat gggagacccttggtcgccagtaaaaaataccgaataagttttaaaaacaatgttgcccagctcaaattttattcagctgagctgcacgacagtggc caatacacatttgagatttccaatgaagtgggcagcagctcctgtgagactacattcactgtattagatcgagacattgctccattttttaccaaaccc ttgcgcaacgtggatagtgttgttaatggtacctgcagactggactgcaaaattgcaggctccctccccatgagggtgtcctggtttaaggatggc aaagaaatagctgcttctgacagatacaggatagcatttgtggaaggcacagcctctttggagatcatcagagtagacatgaatgatgcaggga atttcacttgtcgagccacaaattccgtgggaagcaaagacagcagtggagccctgattgtgcaagaaccacccagttttgtaactaaacccggc tcaaaggatgttctgcctggctcagcagtctgcctgaagagcactttccaaggatctactcctctcacaatcagatggtttaagggcaacaaagag ctggtttctggtggaagctgctatattaccaaagaagctttagagagttccctggaactctatttagtaaaaacctctgattcgggcacgtacacatg taaagtcagcaatgtcgctggaggggtggaatgcagtgcaaacttgtttgtaaaagaacctgccacatttgttgaaaagttagagccatcacagct gttaaagaagggagatgccacccagctagcctgcaaagtaactggtacccctccaattaaaataacatggtttgcaaatgatagagaaattaagg agagcagcaaacacagaatgtcttttgtggagtctactgcagttctaagactgacagatgttggcatcgaagacagtggtgaatatatgtgtgagg cccaaaatgaggctggcagtgaccactgcagtagcattgtaatagtcaaagagtcaccttattttaccaaggaatttaagccaattgaagtcttaaa ggagtacgatgtcatgttgctggctgaggtggcaggcactcctccctttgagatcacttggttcaaagataacacaatcctgcgaagtggtagaa agtataagactttcattcaggatcatctggttagcctgcagatcctcaagtttgtagctgcagatgctggcgaataccagtgtcgggtgaccaatga ggtgggcagcagcatctgcagtgccagggtgactttaagagaacccccatccttcataaagaagatcgagagtaccagctccctccggggag gcacagctgccttccaggccactctgaagggctccttgccaattacggtgacttggctaaaagacagcgatgaaatcactgaagacgataatata agaatgacctttgagaacaatgtggccagtttgtacctcagtggcattgaagtcaagcatgatgggaaatatgtctgtcaggccaaaaatgatgca ggaatacaaagatgttctgcattactctcagtaaaagaacctgcaacaatcaccgaggaagctgtgtctatagatgtcacccaaggagacccag ccactttgcaggttaaattttcagggactaaggagattacagccaaatggtttaaagatggccaagaactgaccctgggctcaaaatataaaatca gtgtcactgatacagtctcaatactgaagattatttctacagaaaagaaagatagtggagaatatactttcgaggtccaaaatgatgttgggaggag cagctgcaaggctagaattaatgtattagatcttatcatacctccttcattcaccaaaaagctgaagaaaatggacagcattaaaggttctttcattga tttagaatgtatagtggctgggtcacatcccataagcatccagtggttcaaagatgaccaagaaatatcagctagtgaaaagtacaaattctcttttc atgacaatactgccttcttggaaatcagccagctggaaggtacagacagtgggacatacacttgttctgccacaaataaggcagggcacaacca atgcagtgggcatctgacagtcaaagaacccccttactttgtggaaaagccacagtcacaagatgtcaatcccaacacaagggtacagttaaag gctcttgtgggtggcactgcacccatgacaataaagtggtttaaagataacaaagagttacactcaggagcagcccgctcagtttggaaggatg acacctctaccagtctagagctctttgcagccaaagctaccgattctggaacctacatttgccaactaagcaatgatgttggcacagcaaccagca aagccactctctttgtaaaagaacctcctcaattcattaagaagcccagtccagtcttagtgctgaggaatggacagtcaacaacatttgaatgcca aataacaggcactcctaaaatccgagtgtcttggtatctagacgggaatgaaataacagccattcagaaacatggcatttccttcattgatggttta gccactttccagatttctggtgccagggtagaaaatagtgggacttatgtgtgtgaagctcgaaatgacgcaggcacggcgagctgcagcattg aactcaaagtgaaagaaccccccacctttatcagagagctgaagcctgtggaggtagtaaaatatagtgacgtggagctggagtgtgaagttac gggaacacctccgtttgaagtcacttggctgaagaataacagggaaattcgaagcagcaaaaaatacacattgaccgacagagtgtctgtgttta acctccatataaccaagtgtgacccttcagacactggggaataccagtgcattgtatccaatgaaggcggcagctgctcatgcagtactagagtt gccctgaaagaaccaccatcattcattaagaagatagaaaataccactactgttttgaaaagttctgccacctttcagagtaccgtggcaggttctc ctcctatttctataacctggctaaaggatgatcagattcttgatgaagatgataatgtctatatttcatttgtggacagtgtggccacacttcaaatcag aagtgtggataatggacacagtgggagatatacctgtcaagccaagaatgagtcaggagttgagaggtgttatgctttccttttagtacaagaacc agctcaaatcgtagagaaagctaaatcagttgatgttacggagaaagatcccatgaccttggaatgtgttgtggctggaacaccagaactcaaag tgaaatggcttaaagacgggaaacaaatagttcccagtagatacttttcaatgagttttgaaaataacgtggccagttttagaattcagtcagtaatg aagcaggacagcggtcagtacactttcaaggtggaaaatgacttcggaagcagtagctgtgatgcctacttaagagtgctagatcaaaatattcc tccatcattcaccaaaaaattaaccaaaatggataaagttctgggctcttctattcatatggagtgcaaggtgtctggttcacttcccattagtgctca gtggtttaaggatggaaaagaaatatctacaagtgcaaaatacagacttgtgtgccatgagagatctgtgtctctggaagttaataatctggaatta gaagatactgcaaattacacatgcaaagtgtcaaatgtagcaggagatgatgcatgcagtggcatcttaactgtgaaagaaccaccaagcttcct agtgaaacctgggcgacagcaagccatacctgattctacagtggaatttaaggcaatactaaaaggaacaccaccatttaaaataaaatggttta aggatgatgtggaacttgtctcaggtcctaaatgtttcattggcttggaagggtcgactagcttcttaaatctctactcagtggatgcttctaagactg gacagtatacttgccatgttaccaatgatgttggtagcgactcttgtactacgatgttgcttgtgacagaaccaccaaagtttgtaaagaaattagaa gcctccaaaattgtgaaagcaggtgactcttcacgacttgaatgcaagatagctggatccccagaaatcagagttgtgtggttccgaaatgaacat gaacttccagccagtgataagtacagaatgactttcattgactcagtggccgtcatacagatgaacaatctcagtactgaggacagtggagatttc atttgtgaggctcagaatcccgctggcagcacaagctgcagtaccaaggttatagtaaaagagccacctgtttttagcagcttccctcctatagta gaaacccttaaaaatgctgaagtcagtcttgaatgtgaactttcgggaacaccaccgtttgaggtggtatggtacaaagacaagcggcaactcag aagcagcaagaaatacaagattgcatccaaaaacttccacacaagtattcacattctcaatgtggacacttcagacatcggtgaataccactgcaa agcacagaatgaagtgggaagtgatacttgtgtttgtactgtaaaattgaaagaaccaccaagatttgtctccaaactgaacagcctcactgttgta gccggagagcctgctgaattacaagcatccatagaaggcgcccagcctatttttgtccagtggcttaaagagaaggaagaagtgattagagaaa gtgaaaacatcaggattacatttgtggaaaatgttgcaactctacagtttgcaaaagcagagccagctaatgctggaaagtatatctgccaaatca agaatgatggtggaatggaggagaacatggccacactgatggtcttagagcccgcagtcattgttgagaaggcaggaccgatgacggtgact gtaggagaaacgtgcactctggagtgtaaggtggctggcactccggaactctctgttgaatggtacaaggatggaaagctgttgaccagcagc caaaaacacaaatttagcttctacaacaaaatctcttccttaaggattctctcagttgaaaggcaagatgcaggcacatacactttccaggttcaaaa taatgttgggaaaagcagctgcacagctgtggttgatgtttcagaccgagcagttcctccctctttcacacgaagactgaaaaatactggtggggt gttaggtgcttcttgcatcttggaatgcaaagtagctggatcatcacctatttcagttgcctggtttcatgagaaaaccaagattgtcagtggagcaa agtaccaaaccacattttcagataatgtctgcacattgcagttgaattctctggattcctcagatatgggcaattacacatgcgtggctgctaatgtcg ctgggtctgatgaatgtcgtgcagtgctaactgtacaagaaccaccctcttttgtgaaagaacctgaacctttggaagtactaccaggcaaaaatg taaccttcacaagcgttattagaggaacccctccattcaaagtcaactggttcagaggtgccagagaactagtgaaaggagaccggtgcaacat ctattttgaagacactgtggcagaactggaattatttaatattgacatatctcagagtggggaatacacctgtgtggtttctaacaacgctggccaag catcttgcactacccgtctctttgtgaaagaaccagctgcatttttgaagagattaagtgatcattctgtagaaccagggaagtccataattctggag agcacctacactggaacacttccaatttctgtcacttggaaaaaggatggttttaacataactacctctgaaaaatgtaacatagtcacaacagaga aaacttgtatcctggaaattctgaatagcacaaaaagagatgcagggcagtattcctgcgagattgaaaatgaggcaggaagggatgtttgtgga gctctggtatctacattagaaccgccttattttgttacggaactggaacctctggaggcagcagttggagattcggtttctttacaatgccaagttgct gggacaccagaaattacagtgtcttggtacaaaggagataccaaattacggccaactcctgaatacaggacctactttacaaacaatgtggccac acttgtttttaataaagtgaacatcaatgacagtggagagtacacatgcaaagcagaaaatagtataggaactgcttcttctaagactgtgttcaga attcaagagcgccaactcccaccttcttttgcaagacaattaaaggacattgaacaaactgtggggttacctgttacactcacttgtcgattaaatgg ctctgcacccatccaagtgtgctggtatagagatggagtacttttaagagacgatgaaaatctacagacttcatttgtagataatgtggcaactttaa aaattttgcaaactgacttgagtcactctggccagtactcttgctcagcttccaacccacttggaacagcatcttctagtgctagactcacagcaag agaacccaagaaatctcccttctttgacatcaagcctgtatctatagatgttattgctggagaaagtgctgattttgagtgtcatgttactggtgctcaa ccgatgcgaatcacttggtcaaaagataacaaggagatccgtcctggaggaaactatacaatcacatgtgtgggaaacactcctcatttgagaat tcttaaagtaggcaaaggagactctggtcaatatacttgccaagcaaccaatgatgttggcaaagacatgtgctcagctcagctcagtgtaaaag aacctccaaagtttgttaagaaattagaagcttcaaaagttgcaaagcagggagaatccattcaactggaatgtaaaataagtggctccccagaa atcaaagtttcatggttccgaaatgacagcgaacttcatgaaagttggaaatacaacatgtcattcattaattctgtggcattgcttacgatcaatgaa gctagtgctgaagacagcggggactacatttgtgaggctcataatggtgttggtgacgccagctgcagcacagcgttgacagtgaaagcacctc ctgttttcacccagaagccttctccagtaggagctcttaaaggttctgatgtgattctccaatgtgaaatttcgggaactcccccatttgaagtagtat gggttaaagatcgaaagcaagttagaaacagcaagaaatttaaaatcacttcaaaacattttgatacaagtcttcatatccttaatcttgaagcctcc gatgtcggggaatatcactgcaaagctactaatgaggtgggaagtgacacgtgttcttgctctgtcaagttcaaagaacctccacgattcgtgaaa aagctaagtgacacctcaacccttattggggatgctgttgagttacgggccatagtggagggcttccagccaatttctgttgtctggctgaaagata gaggtgaagtcatcagagagagtgaaaataccaggatttcattcattgataacattgcaaccctccagctggggagtccagaagcatctaattctg gaaaatatatatgccaaatcaaaaacgatgctggaatgagagaatgctctgcagtcttgactgtactagaaccagcaagaatcatagagaagcc cgaacccatgactgtcactactggaaatccttttgcattagagtgtgtagtgactggaacaccagaactctcagccaagtggttcaaagatggaag agaattgtctgcagacagcaaacatcacattacattcatcaataaagtggcttcccttaaaatcccctgtgccgaaatgagtgacaaaggattatat agctttgaagtgaaaaacagtgttggcaaaagtaactgcactgtatccgtccatgtttctgatcggattgtgcctccttccttcatccgcaagctgaa agacgtgaatgccatcctgggggcctcagttgttttggagtgccgagtctctggctcagccccgatttcagttggctggtttcaggatggaaatga gattgttagtgggcccaaatgtcagtccagcttttcggaaaacgtctgtactttgaatctgagcttgttggagccctccgacacaggcatatacacg tgtgtggctgccaatgtagctggttccgatgagtgctcagcagtcctgactgtgcaagaaccaccatcttttgaacaaacccctgattctgtggaa gttttgcctggaatgagcctcacattcaccagtgtcatcagaggcacccctcctttcaaggtcaagtggtttaaaggcagcagggaactggtgcct ggggagtcatgcaacatctctctggaagattttgtcacagaactggagttgtttgaggtgcagccattagaaagtggagactattcttgcctcgtta caaatgatgctggcagtgcttcctgtaccacacatctttttgtgaaagaaccagccacctttgtgaaaagattggctgatttcagtgttgagacagg aagccccatagttctcgaggccacatacactggcacacctccaatctcagtgagctggataaaggacgagtatcttatttcacaatctgagagatg cagtattactatgacagaaaaatctaccatactggaaattcttgagagcacaatagaggattatgcacagtacagctgcctgatagaaaatgaggc tggtcaagatatctgtgaagctctggtgtctgtcttagaaccaccgtactttattgaacctctggaacatgtggaagcagtcattggagaacctgca actttacagtgtaaagtggatggaactccagaaattagaatttcttggtataaagaacacacaaagctacgatcagctcctgcatataaaatgcaat tcaaaaataacgttgcttccttagtaatcaacaaagtggatcacagtgatgtgggagagtattcatgcaaggcagacaacagtgtgggggcagtc gcttcttcagctgtgcttgttatcaaagagcgcaaacttccacctttctttgcaagaaaactgaaagacgttcatgagactctaggcttcccagttgc atttgaatgccgcatcaatggctcagaacctcttcaagtgtcttggtacaaggatggggttcttttgaaagatgatgctaatttgcagacatcttttgtt cataatgtagcaactcttcagattttacaaactgaccagagccacatagggcagtataattgctctgcttctaatcctcttgggactgcttcatccagt gccaagctcattctctcagagcatgaagtgcctcctttctttgatctaaagcctgtatcagtagatcttgctcttggagaaagtggtacttttaaatgtc atgtaactgggactgcaccaatcaaaatcacttgggccaaagataaccgagagattcgccctggaggcaactacaagatgactttggtagaaaa tactgccactctgacagttctcaaagtaggcaaaggcgatgccgggcagtacacctgctatgcaagcaacatcgctggaaaagactcttgttctg ctcatctgggtgtacaagaaccacccaggttcattaagaagctagaaccttcaagaattgtgaaacaggatgaattcacaaggtatgaatgcaaa atcggtgggtctccagaaatcaaagttttatggtataaggacgagactgaaattcaagagagcagtaaattcagaatgtcattcgttgactcggtg gctgtgctggaaatgcacaatctcagtgttgaagacagtggagactacacctgtgaggcccacaatgcagcaggcagtgccagcagcagcac atccttaaaagttaaagaaccacccattttccgcaaaaagcctcatcctatagagacactgaaaggagctgatgttcaccttgaatgtgagcttcag ggcactcccccatttcacgtttcttggtataaagacaagagagaacttaggagcggcaagaagtacaagataatgtctgagaacttcctaaccag tatccacatcctgaatgtcgatgctgcagacattggggaatatcagtgtaaagccacaaatgatgtgggaagcgacacttgcgttggttccatcgc tctcaaagcaccaccaagatttgtgaagaagctcagtgacatatctactgtcgttggtaaagaagttcagctgcagactaccattgaaggcgctga acccatttcagtggtgtggttcaaagataagggagaaatcgttagagaaagtgacaacatatggatttcttattcagaaaacattgcaaccttacag ttttcaagagtggaaccagccaatgctggaaaatacacttgtcagatcaaaaacgatgctgggatgcaagagtgcttcgccacgctatccgttctc gagcctgcaacaattgtagaaaagccagaatccataaaagttaccacgggagacacctgtaccttggagtgtacagtagctggcacccctgaa ctcagtactaagtggtttaaggatggaaaagagctaacaagtgacaacaaatacaaaataagcttcttcaacaaagtatccggccttaagatcatc aatgtagcaccgagtgacagtggggtatacagttttgaggtgcagaaccctgttggcaaagacagctgcacagcttcattgcaggtttcagaccg aaccgttcctccttcattcacaagaaaattgaaagagacaaatggtctatccggctcctcagttgtaatggagtgtaaagtctatgggtcacctcca atctctgtctcctggttccatgaaggaaatgagatcagtagtggaaggaaataccagaccaccctgacagataatacttgtgctttaactgtgaaca tgctggaagaatcagacagtggtgactacacatgtatagctactaatatggctggttctgatgaatgtagtgctcctttgactgtgagagaaccacc atcttttgttcagaaacctgatcccatggatgttttaactgggaccaatgtaactttcacaagtatcgtaaaaggaacacctcctttcagtgttagctg gttcaaaggtagcagtgaactagtaccaggtgacagatgcaacgtgtctttggaggattcagttgctgaactggagttgttcgatgtagatacatc acaaagtggagaatatacttgcatagttagcaatgaagctggcaaggcttcttgtacaacacatctctacataaaagccccagccaaatttgtgaa gaggctgaatgattacagcatagagaaaggaaaacccctgatcctagagggtacattcactggaactcctcccatttcggttacctggaagaaaa atggcataaatgtaactccttctcagaggtgtaatataactacgactgaaaaatcggcaatcctggaaattccaagtagcacagtagaggatgca ggacaatacaactgctacattgaaaatgcctctggaaaagattcctgttcagcacaaatactcatactagaaccaccgtattttgtcaagcagttgg agccggttaaggtgtctgttggagattctgcctctctacaatgccagcttgctggaacccctgaaattggggtatcctggtataaaggagatacaa aattgagacccactacgacttacaaaatgcattttaggaataatgttgctacactggttttcaaccaggttgatattaatgatagtggagaatatatct gcaaagctgaaaacagcgtgggagaagtttctgcatcaactttccttaccgttcaagagcaaaaacttccaccatcattttctcgacaattgagaga tgttcaagaaacagttggactgccagttgtttttgattgtgccataagtggatcagaacctatctccgtgtcttggtataaagacggcaagccattga aagacagcccaaatgtacaaacatcatttttagacaatacagccacactcaatatttttaaaactgaccggagccttgcaggccagtattcctgcac agctacaaaccctataggctctgcttcttccagtgccaggctcattctcacagaggggaagaacccacccttctttgacatccgtcttgcccctgtg gatgctgtggtgggagaaagtgctgactttgagtgccacgtcacgggcacacaaccgataaaggtcagctgggccaaagacagcagagaaat acgaagtggcggaaagtaccagattagttatctggaaaacagcgcccacctgacagtcctcaaagtagacaaaggagattctggacaatatac ctgctatgctgtgaatgaagtgggaaaagactcttgcacagctcagctgaatataaaagagcggctcatcccaccaagtttcactaaaagactctc agagacagtagaagaaacagaagggaattctttcaaacttgagggacgtgtggctggttcccaacctataactgttgcctggtacaaaaataatat agagatacaaccaacttctaactgtgaaataacattcaagaacaacacgttagtgctgcaagtcagaaaagcaggcatgaacgacgctggtttgt acacatgcaaagtgtccaatgatgcaggctctgctctgtgcacgtcttcaatcgtcatcaaagaacctaagaagccacctgtatttgatcagcacct tactccagtaacagtgagtgaaggagaatacgtgcagctcagctgccatgtccagggatctgagccaattaggatccagtggttgaaggctggc agggaaataaagccttcagacagatgcagcttcagctttgctagtgggacagctgtactggaactcagagatgtggctaaagcagattcgggag attatgtgtgtaaagcttcaaatgtggctggaagtgacactaccaaatcaaaagtgaccattaaagacaaaccagctgtggccccagcaaccaa gaaagctgcggtagatggaagactcttttttgtgtcagaacctcagagtatcagagtcgtagaaaaaaccactgcgaccttcattgcaaaagttgg 004/023933
aggtgacccaatcccaaatgttaaatggacaaaagggaagtggagacagctgaaccaaggaggtcgtgtt±tcatccaccaaaaaggcgatga agcaaaactggagattagggacaccacaaaaactgattctgggttataccgatgcgtggcatttaacgaacatggtgaaattgaaagtaatgttaa cttacaggtggatgaaaggaagaaacaagagaaaattgaaggcgatcttagagcaatgctgaaaaagactccaatcttaaagaaaggagctgg agaagaagaggaaattgatatcatggaacttctcaaaaatgttgatcctaaagaatatgaaaaatatgcccgcatgtatggaatcactgacttccg aggtcttcttcaagcatttgaactgctcaagcaaagccaagaagaagagacacatagactggaaattgaggaaatagagaggtcagagaggga cgaaaaggaatttgaggaacttgtatcatttattcagcaaagactgtcacagacagagcctgtcactctgatcaaggacattgaaaatcagacagt tttgaaagataatgatgctgtctttgaaattgacattaaaattaattatccagaaatcaagctttcgtggtacaaaggaactgaaaaactggaaccca gtgataaatttgaaataagcattgatggtgaccgacatacactcagagtcaaaaactgtcaacttaaagaccagggcaattatcgattggtttgtgg tccacacatcgctagcgctaaactaactgtaattgagcctgcatgggaacggcatcttcaggatgtgactctgaaagaaggccagacttgcacca tgacatgccaattttctgtaccaaatgtaaaatctgaatggttcagaaatggcagaatccttaaaccccaaggtagacataaaacagaagtggaac acaaagtccataaattgaccattgcagatgttcgagcagaagaccagggtcagtacacctgcaaatatgaagaccttgaaacttcagcagaactc agaatcgaagctgaaccaattcagtttacaaagcgcatacagaacatcgtggtgagtgagcatcagtctgccacctttgagtgtgaagtgtccttt gatgatgccattgtaacatggtacaaaggaccaacagaactgacagagagccagaaatacaacttcaggaatgatggccgctgccattatatga ccatccacaatgtgaccccagacgatgaaggtgtctactcggtcatcgctcggctggagccaagaggtgaagcaagaagcacggcagagctg tacctaacgacgaaagaaatcaaacttgagctgaagcctcctgatattcctgactccagagttccaatcccaaccatgcctatcagagcagtgcc accagaagaaatccctcctgtggttgctcctcctatcccccttttgctaccaacacccgaagaaaagaaaccaccaccaaaacgtattgaagttac caaaaaggctgtgaagaaagatgccaaaaaagttgttgcaaagcccaaagagatgacaccacgtgaagagattgtcaagaagcctccacctc ctactaccttaattccagcaaaagctcctgaaatcattgatgtatcctctaaagctgaagaagtaaaaataatgactataaccagaaagaaagaggt tcagaaagaaaaagaagctgtgtatgagaaaaagcaagcagtccacaaggagaagagagtcttcattgaatctttcgaagaaccttatgacgaa ctggaggtagaaccatacacagagccatttgaacaaccttattatgaagaaccagatgaagactatgaagagattaaggtagaagctaaaaaag aagttcacgaggaatgggaagaagattttgaagaagggcaagaatactatgaaagggaagaaggctatgacgaaggggaggaagagtggg aagaggcttaccaagaaagggaagtaattcaagttcaaaaggaggtctatgaagaatcacatgagagaaaagttccagccaaagtacctgaaa agaaagcaccaccacctcctaaagttataaagaagccagtaattgaaaaaattgaaaagacttctcgaagaatggaggaagaaaaagttcaagt caccaaagtacctgaagtttcaaagaagattgttccacaaaaaccttcccggactccagtacaggaagaagttattgaagtgaaagtaccagctg tgcatacaaagaagatggttatttcagaagaaaagatgttctttgcttctcacacagaggaggaggtgtcagtcacagtccccgaggtacaaaag gaaattgttactgaagagaaaattcacgttgccgtttccaaaagggttgaaccaccacctaaagtccctgagctacctgagaaaccagctccaga agaagtggcccctgttcctatccctaaaaaagtggagcccccagcaccaaaagttcctgaggttcccaagaaacccgtgccagaggagaaaa agccagttcctgtgcctaagaaggaacctgctgctcccccaaaagtcccagaggtgccaaagaaacctgtccctgaagaaaagattcccgttcc tgttgcaaagaaaaaggaagctcccccagctaaagttcctgaagtacagaagagagttgtgacagaagaaaaaataaccattgtaactcaaaga gaggaatctccaccaccagcagtgccagaaataccaaagaagaaagttcctgaagaaaggaaacctgttcctcggaaggaggaagaagttcc accaccaccaaaagtgccagctctgcctaagaaacccgtcccagaggagaaagttgcagtgccagttcctgtcgctaagaaagctcctcctcc ccgagctgaagtctctaagaaaactgttgtagaagaaaagagatttgttgctgaagaaaaactatccttcgcagttcctcaaagagtggaagtcac gcggcacgaagtatctgcagaggaggaatggagttactcagaagaggaggaaggtgtgtccatttcagtttatagagaagaagaaagagagg aggaggaagaagcagaggttacagaatatgaagtgatggaagagcctgaggaatatgttgtggaagaaaagctgcacattatttctaagagagt ggaagctgagccagctgaagtgacagagaggcaggagaagaaaattgtactgaaaccaaaaattcctgctaaaatagaggagcctccaccg gctaaagttcctgaagcacctaagaaaattgtgccagaaaagaaagttcctgctccagttcctaaaaaggaaaaggtgcccccacctaaagtgc cagaagagccaaagaaaccagttccagaaaaaaaggttcctccaaaagtcattaagatggaagaacctctcccagccaaagtgactgagagg cacatgcaaattacccaggaagaaaaagttcttgttgctgtaactaaaaaagaggcgcctccaaaagcaagagtgccagaggaaccgaagag agctgtcccagaagaaaaagttctgaaactcaaacctaaaagagaggaggaaccaccagctaaagtgactgaattcagaaaaagagtggttaa agaagaaaaagtatcaattgaagctccaaaaagagaacctcaacccatcaaagaagtaactataatggaagagaaagaaagggcttataccct agaagaagaagctgtttcagtacaacgggaagaagaatatgaggaatatgaagaatatgattataaagaatttgaggagtatgaaccaacagaa gaatatgaccaatatgaagaatacgaggagcgggagtatgaacgatatgaagagcatgaagaatacatcacagaaccagagaagcctatccct gtaaagcctgtcccagaagaaccagttcccacaaaaccaaaggccccaccggctaaagtgctgaagaaagctgtccctgaagaaaaagtacc agtgcccattcctaagaaactcaaacctccaccacccaaagtgcctgaagaaccaaagaaagtttttgaggaaaaaatacgtatttcaattaccaa acgtgaaaaagagcaggtgactgaacccgctgctaaagtgcccatgaagcccaagagggttgtcgcagaagaaaaagtacctgtccctagaa aagaagtagcaccacctgttagagtgccagaagtgcctaaagaacttgaaccagaagaggttgcctttgaagaggaagttgtaacccatgtaga agaatatcttgtagaagaagaagaagagtacattcatgaagaagaggagttcataactgaggaagaagtggtgccagtgataccagtcaaagtg cctgaggtacccaggaaacctgttccagaagagaagaaacctgttcctgttcccaagaagaaggaagctccaccggcaaaagtgcctgaggtt cctaagaagccagaggagaaagttcctgtgcttattcctaaaaaggagaagcctccgccagcaaaagttcctgaagtgcccaagaaacctgtg ccagaggagaaagtaccagtaccagttcctaaaaaggtggaagctccacctgccaaagtgccagaggtacccaagaagcctgtgcctgagaa gaaggtgccagttcctgctcctaagaaagtggaggctccacctgcaaaagtgccagaggtgcccaagaagctcatcccagaagaaaagaaac caacacctgttccgaaaaaagtggaagcaccaccacccaaagtgccaaagaaacgtgaaccagttccagttcctgtagctctacctcaggaag aggaagttctatttgaagaagaaattgttcctgaagaggaagttctacctgaggaagaggaagttctacctgaggaagaggaagttctacctgaa 33
gaagaggaagttctacctgaagaagaggaaattccacctgaggaagaggaagttcctcccgaagaagaatatgtacctgaggaagaagaattt gtacctgaagaagaagtccttccagaagttaaacctaaggtgccagtacctgcaccagtgcctgaaattaagaagaaagtgacagagaagaaa gtggtcattcccaagaaagaggaggctccccctgccaaagttcctgaggtgcctaagaaggtggaagaaaaacgaatcattctccctaaagaa gaggaagttctaccagttgaagtgactgaggagcctgaagaagagcGtatttcagaagaagaaatcccagaagaaccacctagcatagagga agttgaagaggtggcaccacctagagtgcctgaagtgattaagaaagcagtacctgaagcacctactcctgttcctaaaaaagtggaggcacca ccagctaaagtgtcaaagaaaattcctgaggaaaaagtacctgttcctgttcagaaaaaagaggcacccccagccaaagtgcctgaagtacca aagaaagtcccagaaaagaaagtccttgtgcctaaaaaggaagctgttcccccagctaaagggagaactgtccttgaagaaaaagtatcagttg ccttccgccaagaagtagtagtaaaagaaagactagaattagaagtagtagaagcagaagtggaagaaattccggaagaagaagagttccatg aagttgaagaatattttgaagaaggcgagtttcatgaagtagaagaattcatcaaattagaacaacatagagttgaagaagaacacagagttgaa aaagttcatagggtaatagaagtttttgaggctgaagaagtggaagtatttgaaaaaccaaaagctccacctaaagggcctgagatatctgagaa aatcatccctccaaaaaaaccgcccactaaagttgttcctcgaaaagagccaccagctaaagtaccggaggtgcctaagaaaattgtggtagaa gaaaaagtacgtgttcctgaagagcccagagttccaccaactaaagtgcctgatgtgctgccaccgaaggaagtggtcccagaaaagaaagta ccggtgcctcctgccaaaaagccagaagctccacctcctaaagtgcctgaggctcccaaagaagttgttcctgaaaagaaagtgccagtgcctc ctcctaaaaagcctgaagtgccacccacaaaagtcccagaggtgccaaaggcagctgtcccagaaaagaaggtgcctgaagctattcctccca aaccggaaagtcctccccctgaagtgccagaggctccgaaagaagttgttcctgaaaagaaagtgccagcggctcctcctaaaaagcctgaag tcacacctgttaaagtgcctgaggctcccaaagaagttgttcctgaaaagaaagtgccagtgcctcctcctaaaaagcctgaagtgccacccaca aaagtcccagaggtgccaaaggtagctgtcccagaaaagaaggtgcctgaagctattcctcccaaaccggaaagtcctccccctgaagtgttc gaggagcctgaggaagttgccctagaagagcctcctgctgaagttgtggaagagccagagccagcggcgcctccacaagtgaccgtaccac ctaagaaacctgtcccagaaaagaaagcacctgctgtcgttgccaaaaaacctgaactaccaccagtgaaagtgcccgaggtgcccaaggag gttgttcctgaaaagaaggtgcctctggtggttcccaaaaagccagaagccccacctgctaaagtgcctgaagttccaaaagaagttgttccaga aaagaaagtagctgttcccaaaaagccagaagtcccaccagcaaaagtgccagaagtgccaaagaaacctgtcttggaagagaaaccagctg ttcctgttccagaaagagcggagtctcctcccccagaagtatatgaagaacctgaggaaattgctcctgaagaggaaattgctcctgaagagga aaagccagttcctgttgcagaagaggaggaaccagaggttccacctccagcagtgcctgaagagcccaagaagatcatcccagagaagaaa gttcctgtcatcaaaaaaccagaagcaccgcctcctaaagaacctgaacctgaaaaggttattgagaagccaaaactcaaaccaagaccccca cctcctccacctgctccacctaaggaagatgtgaaggagaaaatattccaacttaaagctattccaaagaagaaagttcctgaaaaacctcaggtt ccagaaaaagtggagcttacacctctgaaagtgcctggaggtgaaaagaaagttcgcaaattacttccggaacgtaaacctgaaccaaaggaa gaagttgttctgaaaagcgttctaagaaaaagacctgaagaagaagaacctaaagtagaacctaaaaaactagaaaaagttaaaaaacctgcag taccagaaccaccacctccaaaacctgttgaagaggttgaagtacctactgttacaaaaagggaaaggaagattcctgaaccaacaaaagtgcc tgaaatcaagccagcaatacctctccctgcacctgaaccgaaaccaaagcccgaagcagaagtgaaaacaatcaaaccacctcctgtggaac ctgaaccaacccccatcgctgccccagtaacagtgccagtggttggaaagaaagcagaagccaaagcacctaaggaagaggctgccaagcc aaaaggtcctatcaaaggtgtacccaaaaagactccttcaccaatagaagccgaaaggagaaagttaaggccaggaagtggtggagagaaac ctcctgatgaagccccgttcacctaccagctaaaggctgtgccactgaagtttgtgaaagaaatcaaagacatcatcttgacagaatcagagttcg ttggctcttcagcaatctttgaatgtttggtctccccttccactgcaattacaacctggatgaaagacggtagcaatatccgtgagagtcccaagca caggtttattgcagatggtaaagacagaaagctgcacatcattgatgttcaactttccgatgctggtgaatacacctgtgttttacgtttgggaaaca aagaaaagacctccacggctaaacttgttgtagaagaacttcctgtgcgttttgtaaaaacactggaagaggaagtcacagtggtcaaaggaca gccattgtacttgagctgcgagttaaacaaagagcgtgacgtggtctggaggaaggatggcaagattgtggtggagaaacctggccgaattgt gccaggcgtcattggcttgatgcgggctctgaccatcaacgatgcagatgacacagatgctggaacatacacagttactgtggaaaacgccaa caacctggagtgttcatcttgcgtaaaagtagtagaagtcattagagattggctggtgaaacctatacgagaccagcatgtgaaacccaagggg acagctatttttgcctgtgatatagcaaaagatactccaaacattaagtggttcaaaggatatgatgaaatccctgcggaaccaaatgataagactg aaatactgagagatggaaatcatctgtacctcaaaattaagaatgctatgccagaagatattgctgagtatgcagtggaaattgaaggaaaaagat accctgcaaagctgacacttggagagcgtgaagttgaactgcttaaaccaatagaggacgttaccatttatgagaaagaaagtgcaagctttgat gcagaaatctcagaggcagacattcctggacaatggaaactgaaaggagaacttctaaggccctcacctacttgtgaaatcaaagcagaaggt ggaaaacgcttcttaactttgcacaaagtcaaactggaccaagctggtgaagtcctctaccaggcccttaatgcaattacaactgccattttgacag taaaagaaatcgaacttgactttgctgtgcccctgaaggatgtcactgttccagaaaggcgacaggctcgattcgaatgtgtcctcacccgagag gcaaatgttatatggtccaaaggacctgatataattaagtcatctgacaaatttgatatcatcgctgatggaaagaaacatattcttgttattaatgatt ctcaatttgatgatgaaggggtctatactgctgaggtggagggcaagaagacctcagctcggttgtttgtcacaggtataagactgaaattcatgt cacctcttgaagatcaaacagtaaaagaaggtgaaacagcaacttttgtttgtgaactttctcatgaaaaaatgcatgtagtctggttcaaaaatgat gccaaactccatacaagcagaacagtactcatctcttctgagggcaagactcacaaattggaaatgaaagaagtgacattggatgatatatctca gataaaagctcaagtcaaggagctgagctccacagcacagctgaaggtcttagaggccgatccctacttcactgtgaaattacatgacaaaact gcagtggagaaggatgagattactttgaagtgtgaagtgagcaaagatgtaccagtgaaatggttcaaagatggtgaagagattgtcccttcacc caaatattctatcaaggcagatggcctgcgccgcatcttaaaaatcaaaaaggcggaccttaaagataaaggcgaatatgtgtgtgactgtggca cagacaagaccaaggcaaatgttactgttgaggctcgactaataaaagtggaaaagcctctgtacggagtagaggtgtttgttggtgaaacagc ccactttgaaattgaactttctgaacctgatgttcacggccagtggaagctgaaaggacagcctttgacagcttcccctgactgtgaaatcattgag gatggaaagaagcatattctgatccttcataactgtcagctgggtatgacaggagaggtttccttccaggctgctaatgccaaatctgcagccaat ctgaaagtgaaagaattgcctcttatcttcatcacacctctcagtgatgttaaagtcttcgagaaagatgaggctaagtttgagtgtgaagtatccag ggagcccaaaacattccgttggctaaaaggaacccaggaaatcacaggtgatgacagatttgagcttataaaggatggcactaagcattcaatg gtgatcaagtcagctgcttttgaagatgaagcaaaatacatgtttgaagctgaagataagcacacaagtggcaaactgatcattgaaggaatccg gctcaaattcctcacccctctcaaagatgtaactgccaaagagaaggaaagtgctgtatttactgtggagttatctcatgataacatccgagttaaat ggttcaagaatgaccagcgcctacacaccaccaggtcggtctcaatgcaagacgaagggaaaactcattcgatcacattcaaagacctgtctatt gatgacacctcccaaattagagtagaagctatggggatgagttcagaagctaaactcactgtgcttgagggagacccatattttacaggaaaactt caagattatactggtgtagagaaagatgaagttattctacagtgtgaaattagcaaagcagatgcaccagtgaaatggtttaaggatgggaagga aataaagccatccaaaaatgctgttattaaggcagatggcaagaaacgcatgctaatcctaaagaaagccttgaaatcagatattggacagtaca cctgtgactgtgggacagataagacctcaggaaaacttgacattgaggatcgggaaattaaactggtgcgacccctgcacagtgtggaggtgat ggagactgagacagcacgctttgaaaccgaaatctctgaagatgatatccacgccaactggaaactcaagggagaggccctactccaaacac ctgattgtgaaattaaggaagaaggcaaaatacactcccttgttttgcacaactgtcgcctggaccagacgggtggggtggatttccaagctgcc aatgttaaatctagtgcccacctccgagttaagccacgagtaattggtcttctgaggcctttaaaggatgtcaccgtgactgcaggggaaacagc caccttcgactgcgagctctcctacgaagatatcccagtggaatggtatctcaaagggaagaaactagagcccagcgataaggtggtcccacgt tcagaaggaaaagttcatacacttactctgagggatgtaaagttagaagatgctggggaagtccaactaacagcaaaagatttcaaaactcacgc caacctctttgtgaaagaacccccagttgaattcactaagcctcttgaggaccagacggtcgaagagggagccactgcagtgctggagtgtgaa gtctccagagaaaatgctaaggtgaaatggttcaaaaatgggacagaaatcctcaaaagcaagaagtatgaaattgttgctgatggcagggtca gaaaacttgttatacatgactgtaccccagaggatattaaaacatacacttgtgatgctaaggattttaagacttcctgtaacctgaatgtcgtgcctc ctcatgtggaattcttaagaccactcaccgaccttcaagttagagaaaaagaaatggctcgatttgagtgtgaactttcccgagaaaatgctaaggt taagtggtttaaagatggtgctgaaattaaaaagggcaaaaaatatgacatcatatccaagggagcagtgcgcattcttgtcatcaacaaatgtcta ctggatgatgaagctgaatattcctgtgaagtaaggacagcgagaacttctggcatgctgacagttctggaagaagaagctgtctttaccaaaaat cttgccaacattgaagttagtgaaacagacactataaaactggtttgtgaagtctccaakcctggcgcagaagtgatttggtataaaggggatgag gagatcattgaaacaggaagatatgaaatactgactgaaggacggaagagaatcctggtcattcagaacgctcaccttgaggatgctggcaact acaactgtcgactcccaagctctcgaaccgatggcaaagtcaaagtacatgaactggctgctgaatttatctcaaagcctcaaaaccttgaaatac ttgaaggagaaaaggctgaatttgtctgctctatatcaaaagaaagctttccagtccagtggaagagggatgataagacacttgaatctggagata aatatgacgttattgctgatggtaaaaagagggtcctagttgtgaaagatgccacattacaagatatgggcacttacgttgtcatggtaggggccg ccagagcagcagctcacttgacagtcattgaaaaactcaggatcgtagttcctcttaaggacacccgggtgaaggaacaacaggaagttgtctt caactgtgaagtcaatactgaaggtgccaaagccaaatggttcagaaatgaagaagctatatttgatagttcaaaatacatcattctccaaaaaga cctagtctacaccctcagaattagagatgcacacttagatgaccaagccaactataatgtgtctttgaccaatcacagaggtgaaaatgttaaaagt gcagccaatctaatagtagaagaggaagaccttaggattgttgagcctcttaaagatattgaaacaatggagaagaaatctgtcacattctggtgc aaggtgaatcgtctcaatgtaacactgaagtggaccaaaaatggtgaagaagtgccttttgacaaccgtgtctcatacagagttgataagtacaag cacatgttaaccattaaagactgtggcttcccagatgaaggtgaatacattgtcactgctggacaagataaatctgttgctgagcttctcatcataga agccccgacagaatttgtggaacacttggaagatcagacagtcactgagttcgatgacgctgtcttctcctgccagctctccagagagaaagcc aatgtaaaatggtacagaaatgggagagaaatcaaagaaggcaaaaaatacaaatttgaaaaagatggaagtatacacagactcattataaaag attgcaggctggatgatgagtgtgaatatgcttgcggggtagaagacaggaagtctcgtgctagactttttgtggaagaaattcctgttgagatcat caggcctccacaagatattcttgaagcccctggtgctgatgttgtctttttagcagaactcaataaagataaggtggaagtccaatggctaagaaat aacatggttgttgtccagggtgataaacaccagatgatgagtgaaggaaagatacatcgactacagatttgtgatattaagccccgtgaccaggg tgaatacagatttattgccaaagacaaagaagccagagctaagcttgaactggcagctgcaccaaaaatcaagacagctgaccaagaccttgtg gttgatgttggcaagcctctgacaatggtggtgccatatgatgcctaccccaaagcagaagctgaatggtttaaagaaaatgaacctttatctaca aaaaccattgatactacggctgaacaaacttctttcagaattttagaagccaagaaaggagacaaagggaggtataaaattgtgcttcagaacaa acatggaaaagcagaaggattcatcaatttaaaagttattgatgttcctgggccagtacgtaacttagaagtgacagaaacatttgatggtgaagtg agccttgcttgggaagaacctttaactgatggtggaagcaaaatcataggttacgttgttgaaagacgtgacattaagagaaagacctgggttctg gccacagaccgtgcagagagttgtgagtttactgtcactggtctacagaaaggaggagttgagtacctattccgtgtgagtgcaagaaacagag ttggcactggtgagccagtagaaactgacaatcctgtagaagcaaggagtaaatatgatgttccaggccctcctttgaatgtaaccatcactgatg tgaatcgatttggtgtctGactgacatgggaaccaccagagtatgatggaggtgctgagatcacaaactacgtcattgaattaagagacaagactt ctatcaggtgggatactgccatgactgtgagagctgaagacctgtctgcaactgttactgatgtggtagaaggacaggagtacagtttccgagtg agagcccaaaatcgaattggagttggaaaaccaagtgcagccacacccttcgtcaaagttgctgatccaattgagagaccaagtcctcctgtaa acctaacttcctcagatcagactcagtcatcagttcagctcaaatgggaacctcctctgaaagatggaggaagcccaatattaggctatataattga gcgatgcgaagaaggaaaagataattggattcgttgcaatatgaaacttgtccctgaactgacttacaaggttaccggattggaaaaaggaaata aatatttatatagagtatctgcagaaaataaagctggtgtttcagatccatctgaaattcttggtcctctcaccgctgacgatgcatttgttgaaccaac aatggatttaagtgcatttaaagatggtctggaagttattgtcccaaatcctatcacgatcctggttccaagtacaggctatccaaggccaactgca acctggtgttttggagataaagtactagaaacaggggaccgggtgaaaatgaagaccttgtctgcctatgccgaacttgtcatttctccaagtgaa cgttcagacaagggcatttatacactgaaattagaaaaccgtgtgaaaacaatttctggggaaattgatgtcaatgtaattgctcgcccaagtgcac ccaaagaattgaaatttggtgatataaccaaggactcagtacatttgacttgggaaccacctgatgatgatggaggaagtccgttaactggatacg ttgttgaaaaacgagaagtcagccggaaaacatggactaaagttatggactttgtgactgatctagaattcacagttcctgatcttgttcaaggaaa agagtacttatttaaagtttgtgctcgtaacaaatgtggccctggagaacctgcatatgttgatgaacctgtaaatatgtcaactcctgcaacggtac ctgacccaccagagaatgttaaatggagagatcgaacagccaatagcatcttcttaacatgggatccacctaaaaatgatggtggttcacgcatc aaaggatatatagttgaaagatgtccacgtggttctgataaatgggttgcctgtggagaacctgttgcagaaacaaaaatggaagtgacaggtctt gaggaaggcaaatggtatgcctaccgGgtgaaggccttaaacaggcagggtgctagcaaaccaagcagacccacagaggaaatccaggct gtggacacacaagaggccccagaaatcttcctcgatgtgaagctccttgctggtctcactgtaaaagctgggaccaagattgaacttcctgccac cgtaaccggaaaacctgaacctaaaataacttggacaaaggctgatatgattctgaagcaggacaaaagaattaccattgaaaatgtccctaaga aatccacagtgactattgttgatagtaagagaagtgacactggcacatatatcattgaggctgtgaatgtgtgtggccgggccactgctgtggtgg aagtgaacgtcttagataaacccggaccaccagctgcctttgacatcacagatgtaaccaatgagtcatgtcttctaacatggaacccaGcacgc gatgatggtggatctaagatcacaaactatgttgtggagagacgagcaactgatagtgaagtgtggcacaagctctcatccaccgtcaaggata caaacttcaaggccaccaaattaatccccaataaagagtacatcttcagagttgctgcagaaaacatgtatggtgttggtgaaccagttcaggcct ctccaataacagccaaatatcagtttgatccacctggtcctccaactcgcctagaaccttctgatatcactaaagacgcagtgactctcacatggtg tgagccagatgatgatggtggcagcccaatcacaggatactgggttgaaagactggatcctgatacagataaatgggttagatgcaataagatg ccagtaaaggacacaacatacagagtgaaaggtctcactaataagaaaaaatacagattccgtgtgttggctgaaaatcttgctggacctggaaa accaagcaaatcaactgaaccaatcttaataaaggatcccatagatcctccatggccccctggaaaaccaactgtaaaagatgtaggcaaaaca tcagtaaggttgaattggacaaaaccagaacatgatggaggtgcaaagattgagtcttatgtcattgaaatgctgaagactggaacagatgagtg ggtcagagtggcggaaggggttcccaccactcagcacttgctcccagggctcatggaaggacaggaatactcattccgagttagagctgtgaa taaggctggggaaagtgaacccagtgaacccagtgaccctgtgctttgccgggagaagctatatcctccatcaccaccacgctggcttgaagtt attaatatcacaaaaaatacagcagacctaaaatggacagttcctgagaaagatggagggtcccccatcaccaactacattgtggaaaagagag acgtcaggcgaaaaggctggcaaacagtggataccactgtcaaggacaccaagtgcacagtcaccccactgactgagggctctttatatgtgtt ccgagttgctgcagaaaatgctataggacaaagcgactacaccgaaattgaggactctgtgctggccaaagacacctttaccactcctggacca ccctacgccctggcagtggttgatgtgacaaaacgacatgttgacctaaagtgggagccacctaaaaatgatggtggaagaccaatacagaga tatgtcattgagaagaaagaaaggttaggtacccgttgggtgaaagctggaaagactgcaggacctgactgtaacttcagagtaactgatgtcat cgaaggaacagaggtccagtttcaggttcgggctgaaaatgaagctggagttggccacccaagtgaacccacagaaatcctatccattgaaga tccaacaagtcctccctcaccaccccttgacctacatgtgactgatgctgggagaaaacacattgccattgcttggaagcctccagagaaaaatg gtggaagtcctatcataggataccatgttgaaatgtgtccagtaggcactgagaaatggatgagagttaattctcgcccaataaaggacttgaaatt caaggttgaagaaggtgttgttcctgacaaagaatatgtcctgagagtgagagcagtcaatgctattggtgtcagcgagccatctgaaatctctga aaatgtggttgccaaagacccagactgcaagccaacaattgacctggagactcatgacattattgttattgaaggtgaaaagttaagcattcctgtt cccttcagagctgtcccagttccaactgttagttggcataaagatggcaaagaagttaaagcaagtgatagattaacaatgaagaatgatcacatc tctgcacaccttgaagttcccaagagtgtccgtgcagatgccggaatttataccattacactggagaataagctcggctcagcaacagcctcaatc aatgtcaaagtcataggcctacctggaccatgcaaagatattaaagcaagtgacattaccaagagttcttgtaagttaacttgggaacctccagaa tttgatggtggaaccccaattcttcattatgtcctggagcgcagagaagctgggaggagaacatatataccagtcatgtctggtgagaacaaactg tcatggactgtgaaggatctcataccaaatggtgaatacttcttccgtgttaaagcagtcaacaaggttggtggaggagaatatattgaactgaaaa atccagtcattgctcaagatccaaagcaaccccctgatccacctgtagatgtagaggttcataatcctacagcggaggcaatgactattacatgga agccacctttgtatgatggagggagcaagataatgggctacatcatagagaagattgctaagggtgaagaaaggtggaagagatgcaatgaac acctggtaccaatcctgacctatacagcaaaaggacttgaagaggggaaagagtaccaattccgtgtgcgagcagagaacgccgcgggtatta gtgaaccttctcgggctactcctccaaccaaagctgtagatcccattgatgcccccaaagtcattctgagaacaagcctagaagtgaaacgaggt gatgaaatagcacttgatgcaagtatttctggatcaccttacccaactattacatggataaaggatgaaaatgttattgtaccagaggaaattaagaa gcgtgcagcacccttggttaggagaaggaagggtgaagttcaagaagaagaaccatttgtcctgcctctgacacagcgtttgagtattgacaac agcaaaaagggagaatctcagctacgcgtccgagattctctccgacctgaccatggtctgtatatgatcaaagttgaaaatgaccacggtattgc aaaagctccttgtactgtcagtgtgttagatacaccgggaccaccaatcaactttgtatttgaagatatcagaaagacctcagtcctttgtaaatggg aaccaccccttgatgatggtggcagtgaaatcataaactacactttggaaaagaaagacaagacaaaacccgactcagaatggattgttgtcact tcaacacttagacattgcaaatattcagtaacaaaactgattgaaggaaaagagtacctcttccgtgtaagagctgaaaacagatttgggccaggt ccaccatgtgtttcaaagccacttgtggctaaagatccatttggaccacctgatgcaccagataagcccattgtggaagatgttaccagcaacagt atgctagtgaaatggaatgaaccaaaagataatggaagccccattttgggttactggcttgaaaaacgtgaagttaacagtacacattggtctcgt gtcaacaaaagccttctgaatgccttgaaagccaatgtagatggcttattagaaggactcacctatgtcttcagagtatgtgctgaaaatgcagctg gacctggaaagttcagtccaccttcagatcccaaaacagcacatgatccaatctctcctcctgggccacctatcccaagagtcactgacacaagc tctacaactattgaactagaatgggaacccccagctttcaatggtggtggggaaattgttggctattttgttgataagcagttggttggcacaaatga atggtcacgctgcacagagaagatgatcaaggtccgtcagtacaccgtcaaagaaatccgagagggtgctgattacaaacttcgggtgagtgct gtcaatgccgcaggggaaggaccgcctggagaaacacaacctgttactgtggctgaaccacaagagcctccagctgtggaactggatgtttct gtcaagggtggaatacaaataatggctgggaagactcttagaattccagctgtggtgactggtcgccctgtacctacaaaagtatggaccaaag aagaaggggagctggataaagaccgtgttgtaatagacaacgttggaaccaaatctgaactaattatcaaggatgcactgcgaaaagaccatg gcagatatgtgattacagctacaaatagctgtggttccaaatttgcagcagccagggtagaagtttttgatgtccctggtccagttcttgacttaaaa cctgttgtaacaaacagaaaaatgtgtctacttaactggtctgatccagaagatgatggaggaagtgaaataacaggctttatcattgaaagaaaa gatgccaagatgcatacttggagacaaccaatagagactgagagatctaaatgtgacatcacaggtctgcttgagggacaagaatataagttcc gtgttattgccaagaacaagtttggctgtggccctcctgttgaaataggaccaattcttgcagttgatccactaggtcctccaacatctccagagag gctcacatacactgaaaggacaaagtccactatcacacttgactggaaagagccccgcagtaatggtggcagtcccatccaaggatatatcatt gaaaaacggcgtcatgacaaacctgactttgaaagagttaacaagcgactctgcccaaccacatcttttctggttgaaaatcttgatgaacaccaa atgtatgagttccgtgtcaaagctgtcaatgaaattggtgaaagtgaaccatccctacctcttaatgtagtcatacaagatgatgaagtgcctccaa ctattaagttgcgtctgagtgttcgaggagacactatcaaagttaaggcaggagagcctgtccacatccctgcagatgtgacaggccttccaatg cctaagattgaatggtccaaaaatgaaactgtaattgaaaaacccactgatgcacttcagataaccaaggaagaggtatcccgaagtgaggcaa aaactgagcttagcattcccaaagcggtccgggaggacaaaggcacttacacagttactgcttccaatcgccttggctcagtgttccgaaatgttc acgttgaagtatatgaccgcccatccccaccaagaaatcttgctgttactgacattaaagctgaatcttgctacttgacatgggatgcccctcttgat aatggtggcagtgaaatcacccattatgttattgacaaacgtgatgcaagtaggaagaaagcagaatgggaggaagtcaccaacactgctgtag agaaaagatatgggatctggaaacttatccccaatggtcagtatgagttccgagtcagggcagtgaataaatatggaatcagtgatgagtgcaaa tcagataaagtagtcattcaagatccttatcgccttcctggacctccaggaaaaccaaaagttttggcacgcaccaaaggatcaatgctagtgagc tggactcctcctttggacaatggtggctctccaattactggctactggctggagaaaagagaagagggaagtccttattggtcacgtgttagccga gcaccaataaccaaagtgggattgaaaggcgtggaatttaatgttcctcgtttgcttgaaggcgttaaataccagttcagagccatggcaataaat gctgcaggaattggtcctcccagtgaaccatcagatccagaggttgcaggagatcccatatttccaccggggccaccttcttgcccagaagttaa agataaaacgaagtcaagcatctcactaggatggaaacctccagccaaagatggtggcagcccaatcaaaggatacattgtagaaatgcaaga agaaggtactactgactggaaaagagtaaatgaaccagacaaacttataactacctgtgaatgtgtggtgcctaatctgaaagagctcaggaagt acagattcagagtgaaagctgtcaatgaagctggtgaatctgaaccaagtgatacaactggggagatccctgccactgatattcaagaggaacc agaagttttcattgacattggagcacaggactgtctggtttgtaaagctggctcacagattaggattcctgctgtcatcaagggacgcccaacacc aaaatcatcttgggaatttgatggaaaggcaaagaaagcaatgaaggatggagttcatgacatacccgaagatgcacagctggagactgctga aaactcctcagtaattattattccggagtgtaaacgatctcatacaggcaaatacagcatcacagccaagaataaagcaggacaaaagactgcaa attgcagagttaaagtcatggatgtaccaggcccacccaaagatctgaaagtcagtgatatcacaaggggtagttgcagactttcatggaagatg ccagacgacgatggaggagacaggatcaaaggctatgttattgagaagaggactattgatggaaaagcctggaccaaagtcaatccagactgt ggaagcaccacatttgtagtgcctgatctcctctctgaacagcaatatttcttccgtgtgcgagcagaaaaccgttttggtattggcccacctgtgg aaaccattcagaggaccactgccagagatccgatatatcctcctgatcctcctattaaactcaagattggcctcatcacaaagaacacagtgcatc tgtcatggaaacccccgaagaatgatgggggctcccctgttacccactatattgttgagtgccttgcatgggaccctactgggacaaagaaagaa gcctggaggcagtgcaataagcgtgatgtggaagaactgcaatttactgttgaagacctagtagaaggtggggaatatgaattccgagtcaaag ctgtcaatgctgcaggagtcagcaagccttcagccactgttgggccctgtgactgtcaaagaccagacatgccaccatcaattgatctaaaagaa ttcatggaggttgaagaaggaaccaatgttaacattgtggccaaaattaaaggtgtgccattcccgacactaacctggtttaaagctcctccaaag aagcctgataacaaagaacctgttctctatgacacccatgtcaacaaactggtggtagatgatacttgcactttagttattccgcagtctcgcagga gtgacactggcttatataccatcacagctgtaaataatctgggaacagcatcaaaggagatgagactgaatgtcctgggtcgtcctggccctcca gtgggacccataaaatttgaatctgtttcagcagatcaaatgacactatcttggtttccacctaaagatgatggtgggtctaagattacaaactatgta attgagaaaagagaagctaacaggaagacatgggtccatgtctccagtgaacctaaggagtgcacgtacacgattcccaaattgctagaaggc catgaatatgtattccgaatcatggcccagaataaatatggcattggagaacctcttgacagtgaacctgaaacagcaagaaacctcttctctgtcc ctggagcaccagataaaccaacagttagcagcgtgactcgtaactccatgactgtcaactgggaagagccagaatatgatggaggctctcctgt gacagggtactggctggaaatgaaagacaccacttcaaagagatggaagagagttaaccgagatcctatcaaagccatgactttgggtgtttctt ataaagtgactggtcttattgaaggttccgactatcaattccgggtatatgcaatcaatgctgctggcgtgggtccagcaagtctgccatcagaccc agcgactgctagagatccaattgcccctcctggtcctccatttcccaaagtgacagattggactaaatcatctgcagatctggagtggtctccccc actaaaagatggtggatccaaagtaactggatacatcgttgaatataaagaagaaggaaaagaagaatgggaaaagggtaaagataaagaagt gagaggaacaaagctcgttgtgacaggattaaaggaaggagcattctacaaatttagagttagtgcagtcaacattgctggcattggagaacctg gagaggtcacagatgtcattgaaatgaaggacagacttgtttcacctgaccttcagctagatgccagtgtcagagatagaattgttgtccatgctg gaggggtgatccgaatcattgcctatgtgtctggaaagcctcctccaaccgtcacctggaacatgaatgaaagaaccttacctcaagaagccac cattgagaccacagccattagctcatccatggtcatcaagaactgccagaggagccatcaaggcgtctattctcttcttgccaaaaatgaagccg gagaaagaaagaagacaattattgttgatgtattagatgttccaggtcccgttggaacaccattcctagctcacaacctaaccaatgagtcctgca aactgacatggttttctccagaagatgatggaggctctccaatcaccaattatgtcattgaaaagcgtgaatctgaccgcagagcatggacccca gtgacatatacagttacccgacaaaatgctactgtccagggtctcattcaaggaaaagcctactttttccgaattgcggctgaaaatagtattggca tgggtccatttgttgagacatcagaggcacttgttatcagagagccaataactgtaccagagcgtcctgaagacctggaagtcaaagaagttact aaaaatactgtaactttgacttggaatcctcctaagtatgatggtgggtcagaaattattaactatgtcctagaaagtcggctcattgggactgagaa gttccacaaagttacaaatgacaacttgcttagcagaaaatacactgttaaaggcttaaaagaaggtgatacctatgagtaccgtgtcagtgctgtc aacattgttggacaaggcaaaccatcattttgcaccaaaccaattacttgcaaggatgagctggcacccccaacgcttcacctcgacttcagagat aagctcacgattcgagttggtgaagcttttgccctcactggccgttactcaggcaaaccaaagcctaaggtttcctggttcaaagatgaagctgat gtgctggaagatgatcgcactcatataaagactacaccagcaacacttgctttagagaagatcaaggccaaacgttcagattccggcaaatactg tgtggttgtggagaacagtacaggctctaggaaaggtttctgtcaagttaatgttgttgaccgtcctggaccaccagtaggaccagttagttttgat gaggtgaccaaagattacatggttatctcttggaagcctcctttagatgatggaggcagtaaaatcaccaattatattattgagaagaaggaagtg ggtaaagacgtctggatgccagtgacatctgcaagtgctaaaacaacatgcaaagtttctaaactacttgaaggaaaagattatattttccggatac atgctgaaaatctgtatggaataagtgatcctctggtgtctgattcaatgaaagccaaagatcgtttcagggttcctgatgcacctgatcagccaatt gttacagaagttaccaaagactctgcattagtaacctggaataagccacatgatggaggaaaacccatcacaaactacatcctggaaaagagag aaactatgtctaaacgatgggctagagttaccaaagatcctattcatccatacactaaatttagggttcctgatcttctagaaggatgtcagtatgaat tccgggtttctgcagaaaatgaaattggtattggagatccaagcccaccatccaaaccagtctttgctaaagatccaattgctaaaccaagtccac ctgttaatcctgaagcaatagatacaacatgcaattcagtcgatctaacttggcagccaccacgtcatgatggtgggagcaagattctgggttatat tgttgagtaccagaaagttggagatgaagagtggagaagagccaatcacacccctgagtcatgtcctgaaactaaatataaagtcaccggtcttc gggacggtcaaacctataagtttagagtgttagcagtcaatgcagctggtgaatcagatccagctcatgttccggagccagtcctagtaaaagac aggcttgaaccccctgagttgattcttgatgccaacatggcaagagaacaacacattaaagttggtgatactctaagacttagtgccatcatcaaa ggagtgccattcccaaaagtaacttggaaaaaagaagacagagatgctccaactaaagcaagaattgatgtgactccagttggtagcaagcttg aaattcgtaatgctgcccatgaagatggtggaatttattctttaacagtggagaatccagctggttcaaaaactgtctcagtaaaagtacttgtattag ataaacctgggccacctagagatctggaagtcagtgaaattaggaaagattcatgttaccttacttggaaagaaccactggatgatggtggttctg ttattaccaattatgtggttgagaggagagatgttgccagcgcccagtggtcacctctctcagctacatcaaagaaaaagagtcacttcgctaagc atctgaatgaaggcaaccagtacctcttccgagtagctgcggagaaccagtatggacgtggtccttttgttgaaacaccaaaaccaatcaaggct ttggatcctctccatcccccagggccacccaaggacctgcaccatgtagatgttgacaagactgaagtctccctagtctggaataagccggatcg tgatggtggttctccaatcactggatatttggtagaatatcaagaagaaggcacccaggactggattaaatttaagactgtgacaaacttagagtgt gtggttactggactacaacaaggaaagacctatagattccgtgtaaaagctgaaaacattgtgggtcttggtctccctgacacaactatcccgata gaatgtcaagaaaaactagtgcctccatccgtggagctagatgtgaaattaattgaaggtcttgtggtaaaggctggaaccacagtcagattccct gctattataagaggtgtgcctgttcctactgcaaagtggacaaccgatgggagtgagattaaaaccgatgagcactacacagttgaaacagaca acttctcatcagtacttaccattaagaactgcttaaggagagacactggggaatatcaaatcacagtttccaatgcagccggtagcaaaacagtag ccgtacatcttactgttcttgatgttcctgggccaccaacaggtcctattaatattctggatgttactcctgaacacatgactatctcatggcagccac ctaaggatgatggaggaagccctgtgataaattatattgttgagaaacaagatacaaggaaagacacgtggggtgttgtctcttccggaagcagt aagacaaagctgaaaatcccacatctgcagaagggctgtgaatatgttttccgagttagagcagagaataagataggtgttggtcctccccttga ctccacacctactgttgctaagcataaatttagtcctccgtctcctcctggtaaaccagtggttactgacattactgaaaatgcagcaacagtgtcttg gaccctgccaaaatctgatggtggcagtccaataactggctactatatggaacgtcgagaagtaactggcaaatgggtgagggtcaacaaaac acctatcgctgacctgaagttcagagtgactggactctatgaaggaaatacatatgagtttagagtttttgctgaaaatcttgcaggactaagcaaa ccatccccaagttctgatccaataaaagcttgccggcccatcaaaccacctggaccacctattaatcctaaactgaaagacaagagcagagaaa cagctgatttggtgtggacaaagcctctcagtgatggtggtagccccattctaggatatgtagtggaatgtcagaaacctggcacggcacaatgg aacaggattaataaagatgaactcattaggcaatgtgcctttagggtacctggactaattgaaggaaatgagtacagattccgtataaaggcagct aatattgtaggagagggtgagccaagagaactagcagaatctgtgattgcaaaagatatccttcatcctccagaagtagaacttgatgttacttgtc gtgatgttattaccgtgagagtaggccaaactatccgcattctagctcgagtcaaaggcagacctgaaccagacataacttggactaaggaagg caaagtattggtccgagaaaagagggtggaccttattcaggatctacctcgtgttgagttacaaattaaagaagctgttagagctgatcatggcaa gtatatcatctcagctaagaacagcagtggacatgcccaaggttcagccatcgttaacgtccttgacagacctgggccttgccagaatttgaaggt taccaatgtaaccaaagagaactgtacaatttcttgggaaaacccactagataatggtggctcagaaataacaaacttcatagtagaatatcgcaa accaaaccagaaaggctggtcaattgttgcatcagatgtcactaaacgattaatcaaggccaaccttttagccaacaatgaatactatttccgagttt gtgcagagaataaagtaggtgttgggccaaccatcgaaacaaaaactcccattctggctattaaccctattgacagaccaggtgagcctgaaaa ccttcacattgcagataaaggaaagacatttgtctatctaaagtggcggaggcctgactatgatggtggcagtccaaatctgtcatatcatgttgag agaaggcttaagggctccgatgactgggaaagagtgcataaaggaagcattaaagaaactcactacatggttgacagatgtgttgaaaaccag atttatgagttcagagtgcaaacaaagaatgaaggtggggaaagtgactgggtgaagacagaggaagttgttgtgaaagaagacttacaaaaa ccagtacttgatctgaaattaagtggggtcctaactgtcaaagcaggggacaccattaggcttgaggcaggggttagaggcaaaccattcccag aagttgcatggaccaaggacaaagacgctacagacttaacaagatcaccaagggtcaagattgatacccgtgctgattcatctaaattttctcttac taaagcaaagcgaagtgatgggggtaaatatgtagttacggcaactaacacggctggcagttttgtggcctatgccactgtcaatgttttagataa gcctggtcctgtgagaaatctgaaaattgttgatgtgtccagtgataggtgtactgtttgctgggatccaccagaagatgatggtggctgtgaaatc caaaattatattctagaaaaatgtgagacaaagcgaatggtttggtctacctattctgctactgtcttgacacctggtactacagtaacacgtctcata gaaggaaatgaatatattttcagagtccgtgcagaaaataaaataggcacagggcctccaacagaaagtaaaccagtcatagccaaaaccaag tatgataaacctggtcgccctgatcccccagaagtcactaaagtaagcaaagaagagatgactgtggtttggaatccacctgaatatgatggtgg aaagtctataactggatactttttggagaaaaaggaaaagcattcaacacgatgggtccctgtcaacaagagtgcaatccctgagagacgtatga aagtacagaatctcctcccagaccatgaatatcagttccgtgtcaaggcagaaaatgaaattggaattggagaaccaagcttgccttcaagaccg gtggtggcaaaagaccccatagagccacctggtccaccaaccaatttcagagtggttgatacaaccaaacattccataactcttgggtggggaa aaccagtctatgatggtggtgcaccgatcattggatatgttgtggaaatgagaccaaaaatagcagatgcgtctcctgatgaaggctggaaacgg tgtaatgctgcagcacagcttgtacgcaaggaattcactgttaccagcttggatgaaaaccaggaatatgagttcagggtgtgtgcccaaaacca agttggtattgggcgccctgcagagctaaaggaagctatcaaacctaaagaaatactagaacctccggagattgatttggatgccagcatgagg aaactggtcatagtgagagcaggatgccctattcgtctctttgctatagtgagaggacgaccagcccctaaagtcacttggcgaaaagttggcatt gataatgtggtcagaaaaggacaagttgatctggttgacactatggccttccttgtcatccccaattctacccgtgatgactcaggaaaatattcctt aacacttgtgaacccagcaggagaaaaggctgtattcgtaaatgtcagagtattagacactcctgggcctgtgtctgatttaaaagtttcagatgtc actaaaacatcatgccatgtgtcctgggcccctcctgaaaacgacggtgggagccaagtgacacattatatcgtggagaaacgtgaggcagac agaaagacatggtcgaccgttaccccagaagttaagaaaacaagcttccatgtaaccaatcttgtccctgggaatgagtattacttcagagtaact gctgtcaacgaatatggccctggcgtcccaacagatgtcccaaaaccagtgcttgcatcagatcctctaagtgagccggatcccccaaggaaat tagaagtgactgaaatgaccaagaacagtgccaccttagcctggttacctcccctacgtgatggaggtgctaaaatcgatggctacatcactagtt acagagaagaagagcagcctgcagatcgctggacagagtactcagtggtaaaagatctgagccttgttgtcactggcctaaaggaaggaaag aaatacaaatttagagtagcggccagaaatgctgttggagtcagtttgccaagagaagctgaaggagtgtatgaagccaaagaacaactgttgc caccaaagatccttatgccagagcaaataactatcaaagctgggaaaaaactccgaattgaagcccatgtgtatggaaagcctcatcccacctgt aaatggaaaaaaggagaagatgaagttgtcacatccagccacctggcagtgcataaagcagacagctcttcaattctgatcataaaagatgtga ctaggaaagacagtggttactacagcctcacagcagagaacagttctgggacagacactcagaaaatcaaagttgtagtcatggatgcccccg gcccccctcagcctccatttgacatttctgatatagacgctgatgcttgctccctgtcatggcacatccctctggaggacggaggcagtaacatca ccaattatatagtggagaagtgtgatgtaagccgaggtgactgggtcacggctctagcttcagtcacaaaaacttcctgcagggttggaaagctg atcccaggccaggagtacatcttccgggtccgtgctgaaaaccgatttggcatttcagagcctctcacatctccaaagatggttgcgcagttccca tttggtgttcctagtgaaccaaagaatgcacgagtcaccaaagtcaacaaggactgtatttttgttgcttgggacagaccagatagtgatggaggg agccccattattggttatctgattgaacgcaaggaaagaaacagtttgctgtgggtgaaagccaatgatactcttgtccggtcaactgaatatcctt gtgctggccttgtagaaggtcttgagtattcattcagaatctatgccctaaacaaagctggatccagcccacccagcaaacccacagaatatgtaa ctgcaagaatgccagttgatcctcctgggaaacctgaggttattgatgtcaccaagagtactgtatctctgatctgggctcgtccaaagcatgatg gaggcagtaaaattattggctatttcgtagaagcttgcaaacttcctggtgataaatgggtacggtgcaatactgcacctcaccagattccccagg aagagtacacagctactggcctagaagagaaagctcagtatcaatttagagctattgccaggaccgcggtaaacattagcccaccttctgaacct tctgatccagtgactatcctcgcagaaaatgtccctcccaggatagacctgagtgtggctatgaaatctttgcttactgtgaaagctggaactaatgt ctgcttggatgctactgtttttggtaaaccgatgccaacagtttcttggaaaaaagatggcacactgctaaaaccagcagaaggcataaagatggc catgcagcggaatctgtgcaccttggagctattcagcgtgaaccggaaggactcaggagactataccattactgctgaaaattcaagtggttcta aatcagccaccattaagcttaaagtgttagataaaccgggtcctccagcatctgttaaaatcaacaaaatgtattcagatcgtgctatgctttcttgg gaaccgcctcttgaagatggaggctcagaaatcaccaactatattgttgacaaacgtgaaacaagcaggcccaactgggctcaagtctctgcaa ctgtgcctatcaccagctgcagcgtggagaaacttatagagggccatgagtatcagttccgtatttgtgctgaaaataaatatggagtaggcgatc cagtcttcactgaaccagcaattgccaaaaacccatatgacccaccaggacgctgtgatcctcctgttattagcaacataaccaaagatcacatga cagtcagctggaagccaccagcagatgatgggggctcacccatcactggctatttgcttgaaaagcgggaaacccaggctgttaactggacta aggtcaacagaaaacctattatagaaagaacattaaaagcaacaggtcttcaagaaggtaccgaatatgagttccgtgttacagctataaataaa gctggaccaggcaaacccagtgacgcatccaaggccgcttatgctcgggaccctcagtatcctcctgcgccaccggctttccctaaagtatatg atacaactcgcagctctgtgagtctatcttggggcaagccagcctatgacggcggcagccctatcattggttatctcgttgaagtaaaacgggctg actccgataactgggtgaggtgcaacttaccacagaatctacagaaaacccgctttgaggttactggcctgatggaagacacacaatatcaattc cgtgtgtatgccgttaataagattggatacagtgaccccagtgatgtgccagataaacactatcccaaggacatcttaattccacctgagggagaa cttgatgcggacttaaggaagacactcatattacgtgctggagttactatgagactatatgtaccagtaaaaggacgcccacctccaaagattactt ggtctaaaccaaatgtcaatctaagagacaggattggactggacataaagtcaactgactttgacactttcttgcgctgtgaaaatgtgaacaaata tgatgcaggaaaatatatcttaaccctggagaacagctgtggtaaaaaggaatataccattgttgtgaaagtgcttgatactcctgggccacctgtc aatgtgactgttaaggaaatatccaaagactctgcttatgttacctgggagcctcccattattgatggcggaagccccatcataaactatgtggtac aaaaacgtgatgcagagaggaaatcctggtctacagtgacaactgagtgctccaaaacaagcttcagagtagctaatttggaggagggaaaat cctacttcttccgagtgtttgctgaaaatgagtatggcattggtgatcccggtgaaactcgtgatgctgtcaaagcttcccaaactcctggaccagtt gtggacctgaaagtgaggtctgtatctaagtcatcctgtagcattggctggaaaaagcctcacagtgatggtggaagtcggattattggatatgta gttgatttcctgactgaagaaaataagtggcaacgagttatgaaatccttaagcctacagtactctgcaaaagatttgactgaagggaaggaatat accttcagagtgagtgctgagaatgaaaatggagaaggaaccccaagcgaaatcactgttgtggcaagggatgatgttgtggctcctgatcttg acttaaagggtctacctgatttgtgctacttggctaaagaaaacagcaacttccggcttaagatccccataaaaggcaagccagctccatcagtct cctggaagaaaggggaagatcctctagcaactgacactagagtcagtgttgagtcatctgcggttaacacaactcttatagtgtacgattgccaaa aatctgatgctggaaaatacacaatcacacttaagaatgttgctggcaccaaggaaggaactatctccataaaggttgttggcaagcctggcatc cccactggaccaatcaaatttgatgaagtcacagcagaagccatgaccttaaagtgggctcctccaaaggatgatggaggttctgaaatcacca actatatcctagagaagagggattctgtgaacaacaagtgggtgacgtgcgcctcagctgtccagaaaaccacctttagagtaaccagacttcat gagggcatggaatataccttcagggtcagtgccgaaaataaatatggtgtaggggaaggcctgaaatcggagccaattgttgcgagacatccat ttgatgtgcctgatgctcccccacctcccaatattgtggatgtcagacacgattcagtatctctaacttggactgaccccaagaaaactggtggttct ccaattacagggtatcatctcgagttcaaggaaagaaacagccttttgtggaagagagctaacaagactccgataaggatgagagactttaaagt gacaggattaactgaaggtcttgaatatgaattccgagttatggcaatcaatttagcaggtgtgggcaagccaagcctaccatcagagcctgttgt ggcactggacccaattgatcctcctggaaaacctgaggttattaacataacaaggaattcagtgactctcatttggactgaacctaaatatgacggt ggtcataagttaactggatatatagtggagaagcgagatctaccttcgaagtcttggatgaaagccaaccatgttaatgtcccagaatgtgccttta ctgtaactgaccttgttgagggtggaaaatatgaattcagaattagagcaaagaatacagcaggtgctatcagtgctccatcagaaagtacagaa accattatttgcaaggatgaatacgaggcaccaacaattgtccttgatcccacaataaaagatgggctaacaattaaagcaggggataccattgtt ttgaatgccattagcattcttggcaaaccccttccaaaatcaagttggtccaaggcaggaaaagacattagaccatcagatatcactcagataactt caaccccaacatcttccatgcttactatcaagtatgccactagaaaagatgcgggtgaatataccatcactgctaccaatccttttggcacgaaggt ggaacatgtgaaggtaacagtccttgatgtacctggtcccccaggtcctgttgaaatcagtaatgtttctgctgaaaaagcaacacttacatggac acctcccttggaagatggcggctcaccaattaagtcctatatacttgaaaagagagaaaccagccgacttttgtggacagtggtttctgaagatatt cagtcttgcaggcatgtggcaaccaaacttatcGaaggaaatgagtacatcttccgggtctcagctgtaaaccactatggcaaaggagaacctgt acagtctgaacctgtcaaaatggtagacagatttggtccccctggccctcctgaaaaaccagaggtatcaaatgtcactaagaacactgccactg tcagctggaaaaggccagtggatgatggtggcagcgaaattacaggatatcatgtagaaaggagagaaaagaaaagcctgcgatgggtgag agcaataaaaacaccagtttccgatctcaggtgcaaagtaacaggactgcaagaaggaagcacctacgaattccgtgtcagtgcagaaaacag agcaggaattggtccacccagtgaggcttcagattctgttctgatgaaagatgcagcatatcctccaggaccaccttcaaatccgcatgtcactga tactaccaagaaatctgcttctttggcatggggcaagcctcattatgatggtggacttgaaatcactggctatgtcgtggagcatcaaaaagtagg agacgaggcctggataaaagataccacaggaaccgccctcagaatcactcagttcgttgttcctgatcttcagactaaagaaaaatacaacttca gaatcagtgccatcaacgatgcaggtgttggggagccagcggtgattccagatgttgaaatcgtagaacgggagatggctcctgattttgaacta gatgccgagcttcgaagaacacttgttgttagagcaggactcagtattaggatatttgtgccaattaaaggtcgtcctgctcctgaagtgacatgga ccaaagataacatcaacctgaaaaaccgagccaacattgaaaatacggaatcatttactcttctgattatcccagaatgtaacagatatgataccg . gtaaatttgtcatgaccattgaaaacccggctgggaagaaaagtggctttgtgaacgtcagagtcttggacacgccaggcccagtcctcaacctg cggcctacagacatcacaaaggacagtgtcaccctgcactgggacctccctctgatagatggaggctcacgtataacaaactacattgtagaga aacgtgaagcaacacggaaatcttattccacagccaccactaagtgccataaatgcacatataaagttaccggcttgtctgaagggtgtgaatatt tcttcagagtgatggcagagaatgaatatggaattggtgagccaacagaaactacagagcccgtaaaagcctctgaagcaccatctccaccag acagccttaacatcatggacataactaagagcaccgtcagcctggcatggcctaagcccaaacacgatggtggcagcaagatcactggctatg tgattgaagcccaaagaaaaggctctgaccagtggacccacatcacaaccgtgaaagggttagaatgtgttgtgaggaatctaactgaaggag aggaatataccttccaagtgatggcagtgaacagcgcggggagaagtgcccctagagaaagcagacccgtcattgtcaaggagcagacaatg cttccagagctggatctccgtggcatctatcagaaactggtcattgccaaagctggtgacaacatcaaagttgaaattccagtgctcggtcgaccg aagcccacagtgacatggaaaaaaggagaccaaattcttaaacagacacagagagttaattttgaaaccacagcgacttcaaccattttaaatat caatgagtgtgtcagaagtgatagtgggccctatccattaacagcaaggaacattgtaggagaggttggtgatgtcatcaccattcaagtccatga tatcccagggccacctactggaccaatcaaatttgatgaagtttcatctgattttgtaaccttctcttgggacccacctgagaacgatggtggtgtac caataagcaactatgtagtggaaatgcggcagactgacagtactacctgggttgagttagcaaccaccgttatacgtactacctataaagccacc cgccttactactggattagagtatcagttccgtgtaaaagctcagaatagatatggagttggaccaggcatcacatcagcatgcatagttgccaac tatccatttaaggttcctggacctcctggtacccctcaggtaactgcagttaccaaggattcaatgacaattagctggcatgagccactttctgatgg tggaagccccattttaggatatcatgttgaaagaaaagaacgaaatggtattctctggcagactgtgagcaaagctttagtaccaggcaacattttc aaatcaagtggacttacagatggtattgcttatgagttccgggtgattgcagaaaacatggcaggcaaaagtaagccaagcaagccatcagaac ctatgttggctctggatcccattgacccacctggaaaaccagtacctctaaatattacaagacacacagtaacacttaaatgggctaagcctgaat atactgggggctttaaaattaccagttatatcgttgaaaagagagaccttcctaatggacggtggctgaaggccaacttcagcaacattttggaga atgaatttacagtcagtggcctaacagaagatgctgcatatgaattccgtgtgatcgccaaaaatgctgcaggtgccatcagtccaccatctgagc catctgatgctatcacttgcagggatgatgttgaggcaccaaagataaaggtggatgttaaatttaaggacacggttatattaaaagcaggtgaag cattcagactggaagctgatgtttcaggccgcccacctccaacaatggaatggagcaaagatggaaaagagctggaaggcacagcaaagtta gaaataaaaattgcagatttctctactaatctggtaaacaaagattcaacaagaagggatagtggtgcctatacccttacagcgactaatcctggtg gctttgctaaacacattttcaatgtcaaagttcttgacagaccaggcccacctgaaggacctttggctgtaactgaagtgacatcagaaaagtgtgt actatcatggttccctccactggatgatggaggtgccaaaattgatcattacatagtacagaaacgtgaaaccagcagattggcatggacaaatgt agcctcagaagtccaagtaacaaagctaaaggtcactaaactcttgaaaggcaatgaatacatattccgtgtcatggctgtaaataaatatggagt gggagagccactggaatcagagcctgtgcttgcagtgaatccttatggaccccctgatccgcccaaaaaccctgaagtgacaactattactaaa gattcgatggttgtctgctggggacatcctgattctgatggtggaagtgaaatcatcaattatattgtggaacggcgtgataaagctggccaacgct ggattaaatgcaacaaaaaaactcttactgatttaagatataaagtgtctggactgacagaaggacatgaatatgagttcaggattatggctgaaaa tgctgctggaattagtgcaccaagtcctaccagtccattttacaaggcttgtgacactgtgtttaaacctggaccaccaggtaacccacgtgttctg gatacaagcagatcatccatttcaatcgcttggaataaacctatctatgatggtggttcagaaatcactgggtatatggttgagattgccctgccaga ggaagatgaatggcagattgtcactccaccagcaggactcaaggcaacttcgtatactatcactggcctcacagagaatcaggaatataagatc cgcatctatgccatgaattccgaaggacttggggaacctgcccttgttcctggaactccaaaggctgaagacagaatgctgcctccagaaattga actggatgctgacctgcgcaaagttgttactataagggcctgctgcaccctgagactttttgttcccatcaaaggaaggcctgcacctgaggtgaa gtgggcccgggaccatggagaatctttagataaagctagcatcgaatccacaagctcttacaccctgcttattgttggaaatgtaaacagatttga cagtggcaaatatatactaactgtagaaaatagttcaggcagcaagtctgcatttgtcaatgttagagttctcgatacaccaggccccccacagga tctgaaggtaaaagaggtcactaagacatctgtcacactcacatgggacccacctctccttgatggaggttcaaaaatcaagaactatattgttga aaagcgggaatcaacaagaaaagcatattcaactgttgcaacaaactgccacaagacttcctggaaggtagaccagcttcaagaaggctgtag ctactatttcagggttctcgcagaaaatgaatatggcattgggctgcctgctgaaaccgcagaatctgtgaaagcatcagaacgacctcttcctcc aggaaaaataactttgatggatgtcacaagaaatagtgtgtcactctcttgggagaaaccagagcatgatggaggcagccgaattctaggctaca ttgtggagatgcagaccaaaggcagtgacaaatgggccacgtgtgccacagtcaaggtcactgaagccactatcactggattaattcagggtga agaatactctttccgtgtttcagctcagaatgaaaagggcatcagtgatcctagacaactgagtgtgccagtgatcgccaaagatcttgtcattcca ccagccttcaaactcctgttcaatactttcactgtactggcaggtgaagacctaaaagttgatgttccattcattggccgccctaccccagctgtaac ctggcataaagataatgtaccactgaagcagacaactagagtaaatgcagagagcacagaaaataattcactactgacaataaaggacgcctg ccgagaagatgttggccattatgtggttaaactgactaactcagctggtgaagctattgaaacccttaatgttatcgttcttgacaaaccagggcctc caactggaccagttaaaatggatgaagtgacagctgatagtattactctttcctggggcccacccaagtatgatggtggaagttctatcaataatta cattgttgagaaacgggacacttccacaaccacctggcaaattgtatcagctacagttgcaaggacaacaataaaggcttgcagactgaagact ggatgtgaatatcagtttagaattgcagctgaaaacagatatgggaagagtacctacctcaattcagagcctactgtagcccaatatccattcaaa gttcctggtcctcctggcactccagttgtcacactgtcctccagggacagcatggaagtacaatggaatgagccaatcagtgatggaggaagta gagtcattggctatcatctagaacgcaaggaaagaaatagcatcctctgggttaagttgaataaaacacctattcctcaaaccaagtttaagacaa ctggccttgaagaaggtgttgaatatgaatttagagtctctgcagagaacatcgtgggcattggcaagccgagtaaagtatcagaatgttatgtgg ctcgtgacccatgtgatccaccaggacggccagaggcaatcattgtcacaaggaattctgtgactcttcagtggaagaaacccacctatgacgg tggaagcaagatcactggttatattgttgagaagaaagaattacctgagggccgttggatgaaagccagttttacaaatattattgacactcattttg aagtaactggcctagttgaagatcacagatatgagttccgggttatagcccgaaatgccgcaggagtgtttagtgagccttcagaaagcacagg agcaataacagctagagatgaggtagatccaccacgaataagtatggatccaaaatacaaagacacaatcgtggttcatgctggtgaatcattca aggttgatgcagatatttatggcaaaccaataccaaccattcagtggataaaaggtgatcaggagctttcaaacacagctcgattagaaataaaga gcaccgactttgccaccagtctcagtgtaaaagatgcagtacgtgtcgacagtggaaattacatactgaaggccaaaaatgttgcaggagaaag atcagttactgtgaatgtcaaggttcttgacagaccagggccacctgaaggacctgttgttatctcaggagttacagcagaaaaatgcacactagc ttggaaacccccacttcaggatggtgggagtgacatcataaattatattgtggaaaggagagaaaccagccgcttagtttggactgtggttgatgc caatgtgcagactctcagctgcaaggttactaagcttcttgaaggcaatgaatatactttccgtataatggcagtaaacaaatatggtgttggtgaac ctcttgaatctgagccagtagttgccaagaatccatttgtagtaccagatgcaccaaaagctccagaagtcacaacagtgaccaaggactcaatg attgttgtatgggaaagaccagcatctgatggtggtagtgaaattcttggatatgttcttgagaaacgggataaagaaggcattagatggacaaga tgccataagcgtctgattggagagttgcgcctgagagtaactggactcatagaaaatcacgattatgagttcagagtttctgctgagaatgctgctg gacttagtgaaccaagccctccttctgcttaccaaaaggcttgtgatcctatttataaaccaggacccccaaacaaccccaaagtcatagacataa ccagatcttcagtattcctttcttggagcaaaccaatatatgatggtggctgtgaaattcaaggatacattgttgaaaaatgtgatgtgagtgttggtg aatggacaatgtgcactccaccaacaggaattaataaaacaaacatagaagtagagaagctgttggaaaagcatgaatacaacttccgtatctgt gctattaataaagctggagttggagaacatgctgacgtccctggacctattatagttgaagaaaaattagaagcaccagacattgatcttgaccta gaactaaggaaaatcataaatataagggcaggtggctccttaaggttatttgttcctataaaaggtcgtcctacaccagaagttaaatggggaaag gtggatggtgaaatccgagatgcagctataattgatgtcactagcagtttcacctctcttgttcttgacaatgtcaaccgatatgatagtggaaaatat acgcttacattagaaaacagcagtggaacaaagtctgcctttgttactgtgagagttctggacacgccaagtccacctgttaacctgaaagtcaca gaaatcaccaaagactcagtatcaattacatgggaacctcctttgttggatgggggatccaaaataaaaaattacattgttgagaaacgtgaagcc acaagaaaatcatatgctgctgttgtaactaactgccataagaattcttggaaaatcgatcagctccaagaaggttgcagttattactttagagtcac agctgagaatgagtatggtattggccttcctgcccagactgctgatccaattaaggttgcagaagtgccacaacctcctggaaaaataactgtgg atgatgtcaccagaaacagtgtctctctgagttggacaaaacctgaacatgatggtggcagtaaaatcattcagtatattgtggaaatgcaagcta aacaGagtgagaaatggtcagagtgtgctcgagtaaagtctcttcaggcagtaattaccaacctaactcaaggggaagaatatctttttagagttgt tgctgtaaatgaaaaggggagaagtgatcctcggtcccttgcagttccaatagttgccaaagatctggtaattgagccagatgtaaaacctgcatt cagtagttacagtgtacaggttggccaagatttgaaaatagaagtgccaatttctggacgtcctaagccaaccattacctggactaaagatggtct cccactgaagcagaccacaagaatcaatgttaccgattcactggatctcaccacactcagtattaaaga,aactcataaggatgatggtggacaat atggaatcacagttgccaatgttgttggtcagaagacagcatccatcgaaattgtaactctagataaacctgatcctccaaaaggacctgttaaattt gatgacgtcagtgctgaaagtattacattatcttggaaccctccattatatacagggggctgccaaatcaccaactacattgttcagaaaagagata caaccaccacagtatgggatgttgtttctgctactgttgctagaactacactcaaagtgaccaaactgaaaactggtacagaataccaatttagaat atttgccgaaaacagatatggacaaagctttgccttagagtctgatccaattgtagctcaatatccctacaaagaaccaggccctccaggtacacc atttgccacagccatttccaaagactccatggtcatacagtggcatgaaccagtcaacaatggtggaagccccgtcataggttaccacctggaga gaaaagaaagaaacagtattttgtggacaaaggtcaacaaaactattattcatgacacccaattcaaagcacagaatcttgaagaaggcattgaat atgaattcagagtgtatgctgaaaatattgttggtgtaggcaaagcaagcaagaattctgaatgctatgtagccagagatccctgtgacccaccag gaaccccagaaccaataatggttaaaagaaatgaaatcactttacagtggaccaaacctgtgtatgatggtggaagtatgattacaggctacattg tagagaaacgtgatttgcctgatggtcgttggatgaaagctagctttacaaatgtcattgaaactcaatttactgtgtcaggtcttactgaagatcaaa gatatgaattcagagtcattgcaaagaatgcagctggtgcaataagtaaaccctctgacagtactggaccaataactgccaaggatgaggttgaa ctcccaagaatttcaatggatccaaaattcagagacacaattgtggtaaatgctggagaaacattcagacttgaggctgatgtccatggaaagcc cctacctaccattgagtggttaagaggagataaggaaattgaagaatctgctagatgtgaaataaagaacacagatttcaaggctttacttattgta aaagatgcaattagaattgatggtgggcagtatattttaagagcttccaatgttgcaggttctaagtcattcccagtaaatgtaaaagtattagataga ccaggacctccagaagggccagtccaggttactggagtcacttctgaaaaatgctctttaacatggtctccaccacttcaagatggtggcagtga catttctcactatgttgttgaaaagcgagaaaccagtcgacttgcctggactgttgttgcttcagaagttgtgaccaattctctgaaagttaccaaact cttagaaggtaatgaatatgttttccgtataatggctgtcaacaaatatggtgttggagagcctttggaatctgcaccagtactaatgaaaaatccatt tgtgcttcctggaccaccaaaaagcttggaagtcacaaatattgccaaagactccatgaccgtctgttggaaccgtccagatagtgatggtggaa gtgagattattggttacattgtagagaaaagagacagaagtggcattcgatggataaaatgtaataaacgccgcattacagatttgcgtctaagag tgacaggattaacagaagatcatgagtatgaattcagggtctctgcagaaaatgctgctggagttggggaaccaagtccagctacagtttattata aagcctgtgatcctgtgttcaaacctggcccacctaccaatgcacacattgtagacaccactaaaaattcaatcacacttgcctggggtaaaccca tctatgatggcggcagtgagatcttgggatatgtagtagaaatctgtaaagcagatgaagaagaatggcaaatagttactccacagactggcctg agagtcactcgatttgaaatttcaaaactcactgaacaccaagagtataaaatacgagtctgtgccctcaacaaagttggtttaggtgaggctacat cagttcctggtactgtgaaaccagaagataaacttgaagcacctgaacttgaccttgactccgaattaagaaaaggaattgttgtaagagctggtg gatctgccagaattcacattccattcaaaggtcgtccaacgcctgagatcacttggtctcgagaggaaggtgaattcacagataaggtccaaattg aaaagggagtaaactatacccaactatcaatagataactgtgatagaaatgatgctggaaaatacattcttaagttggaaaacagcagtggatcaa agtctgcttttgtaactgtgaaagttcttgacactccaggaccaccacagaatttggcagtcaaagaagtgagaaaagattctgccttcctggtatg ggagccacccatcattgatggaggggcaaaggtcaagaactatgtgattgacaaacgtgagtcaaccagaaaagcgtatgctaatgtgagtagt aaatgcagcaaaacaagttttaaagtggaaaaccttacagaaggagccatttattacttcagagtcatggctgaaaatgaatttggagttggtgttc cagtggaaactgttgatgccgtgaaagctgctgaacctccttccccaccaggaaaggttacactcactgatgtgtcccagaccagtgcatcactt atgtgggagaaacctgaacatgatggcggtagcagagtcctggggtacgttgttgaaatgcagcccaaaggaactgaaaaatggagcattgtg gctgaatccaaagtctgtaatgcagttgttactggtttgagttctggacaagaatatcagttccgtgtcaaggcttataatgagaaaggaaaaagcg atccaagagtgttgggtgttcctgtcatagccaaggacttgactatacagcctagtttaaagttaccatttaacacatatagtatccaagctggagaa gatcttaaaatagaaattccagttataggccgaccaagacctaacatttcttgggtcaaagatggtgagcctcttaaacagacaacaagagtaaac gttgaagaaacagctacctcaactgttttgcacattaaagaaggtaacaaagatgactttggaaaatacaccgtaacggcaacaaatagtgcagg cacagcaacagaaaatctcagtgttatcgttttagaaaagcctggacctccagttggcccagttcggtttgatgaagttagtgcagactttgtagtc atatcttgggaacctccagcctatactggtggctgccaaataagcaactacattgtagagaagcgagatacaaccaccaccacttggcacatggt atcagcaacagttgcaagaacaacaattaaaataaccaaactgaaaacaggcacggagtaccagtttagaatttttgctgaaaacaggtatggaa aaagtgccccactggattctaaggcagttattgtacaatatccatttaaagaacctggaccacctggaactccttttgtgacatcaatctcaaaagat cagatgcttgtgcaatggcatgagccagtgaatgatggaggcaccaaaattattggctaccatcttgaacagaaagaaaagaacagtattttatg ggtcaagttaaataagacccccattcaggacaccaaattcaaaacaactgggcttgatgagggccttgagtatgagttcaaagtttctgctgaaaa tattgttggcattggcaagcctagcaaagtgtcagaatgctttgttgctcgtgatccatgtgacccacctggtcgccctgaagccattgttattacaa gaaacaatgtcacactgaaatggaagaaacctgcctatgatggtggtagcaaaataacaggttatattgtagaaaagaaagatctacctgatggc cgctggatgaaagccagctttaccaacgtattagaaactgaatttacagtgagtggacttgtagaagaccaaagatatgaatttagagtaattgca agaaatgcagctggaaactttagtgaaccatctgatagtagtggtgccattactgcaagagatgaaattgatgcaccaaatgcctctctggatcca aaatataaagatgtcatcgttgttcatgcaggagagacttttgttcttgaagccgacatccgtggcaaacctatacctgatgttgtttggtcaaaagat ggaaaagaacttgaagaaacagctgctagaatggaaattaaatctactattcagaaaacaactcttgttgtcaaagactgtatacggactgatgga ggacaatatattctgaaactcagcaatgttggtggtacaaagtctatacccatcactgtaaaggtacttgacaggccagggcctcctgaagggcct ctgaaagttactggagttactgcggaaaaatgttacctggcatggaacccacctttgcaagatggtggtgctaatatttcacattacatcattgaaaa gagggagacaagccgactctcttggacccaggtttcaactgaggtacaggcccttaactacaaagttactaaacttcttcctggtaatgagtacatt ttccgtgtcatggctgtgaataaatatggaattggagagcccttggaatctgggcctgttacggcctgtaatccttataagccaccaggtcctccct caacacctgaagtctcagcaatcaccaaagattctatggtagtaacatgggcacgcccagtagacgacggaggtaccgaaattgagggctaca ttcttgaaaaacgagataaggaaggcgttagatggaccaagtgcaacaagaaaacattaacggatctgcggctcagggtaactggtcttaccga aggccattcctatgaattcagagttgctgctgaaaatgcagctggtgtgggagaacctagtgagccatctgttttctaccgtgcgtgtgatgccttgt atccaccaggtcccccaagcaatccaaaagtgacggacacttccagatcttctgtctccctggcatggagtaagccaatttatgatggtggcgca cctgttaaaggctatgttgtagaggtcaaagaagctgctgcggatgaatggacaacctgcactccaccaacaggattacaaggaaagcagttca cagtgaccaagcttaaagaaaacactgaatataacttccgtatttgtgccatcaattctgaaggtgtaggtgaacctgcaactctacctggctcagt ggttgctcaggagaggatagagccaccagaaatagaactcgatgctgatctcagaaaggtggtcgttctgcgtgcaagtgctactttacgcttatt tgtcactatcaaaggtcgaccagaacccgaagttaaatgggaaaaggcagaaggcattctcactgacagggctcagatagaggtgaccagctc atttacaatgttggtgattgataatgttaccagatttgacagtggtcggtataatctgacattagaaaataatagtggGtccaaaacagcttttgttaac gtcagagttcttgactcaccaagtgcccctgtgaatttgaccataagagaagtgaagaaagaGtcagtgacgttgtcctgggaaccaccacttatt gatggtggagctaagattacaaactacattgtcgaaaaacgagaaactacaagaaaagcctatgctaccattacaaataattgcactaaaactact ttcagaattgaaaatctacaagaaggatgttcttactacttccgagtcttggcttccaatgaatatgggattggtttgccagctgaaacaacagaacc cgttaaagtgtctgaaccacccctcccacctggaagagtaactcttgttgatgtgacccgtaatacagctacaattaagtgggagaaaccagaaa gtgatggtggcagcaaaattactggttatgtggttgaaatgcagactaaagggagtgaaaagtggagcacctgcacacaagttaagactctaga agcaactatatctggcttaactgcaggagaagagtatgtcttcagggtagctgcagttaacgaaaagggaagaagtgatccaagacaacttgga gtgcGagtaattgcaagggatattgaaataaagccttcagttgagcttcctttccatactttcaatgtaaaggctagagaacaacttaagattgatgt gccattcaaaggaagacctcaagctactgtgaactggagaaaagatggtcagactcttaaagagacaactagagtcaatgtttcttcttcaaagac tgtaacatcactatctattaaggaagcttcaaaggaagatgttggaacttatgaattatgtgtttcaaacagtgctggatccataacagttcctattact ataattgtccttgacagaccaggacctccaggtcctatacgtattgatgaggttagttgtgacagcataaccatttcttggaatcctccagaatatgat ggtggctgccaaattagcaattacattgttgaaaagaaagaaaccacctctacaacatggcacatagtttcacaagcagttgcaagaacatccatt aaaatagttcgcctgacaacaggaagtgagtatcagttccgtgtttgtgcagaaaaccgctatggaaagagctCGtacagtgaatcttcagctgtt gttgcagagtatccattcagtcccccaggtcctcctggtactcctaaagttgtgcatgccacaaaatctaccatgcttgtaacctggcaagtgccag ttaatgatggaggaagtcgagtaattggctatcatcttgagtataaagaaagaagcagcattctttggtcaaaagcaaataaaatcctcattgctgat actcaaatgaaagtctccggccttgatgaaggactgatgtatgagtatcgtgtatatgctgaaaatattgctggaattggtaaatgcagtaaatcttg tgaaccagtccctgcaagagatccttgtgaccctcctggacaacctgaagtcacaaatatcacaagaaaatcagtgtcacttaaatggtctaaacc acattatgatggtggagctaagatcacaggatacf»tt£ttgaacgCΛg-Ξ"2ctaccagatggccggtggctgaagtgcaattatactaatatac aagaaacatactttgaagtaactgaacxtactgaagatcagcgttatgaattccgggtttttgcaaggaatgctgctgactcagttagtgagccatct gaatccactgggcctattatagttaaagatgatgttgagcctccaagagttatgatggatgtcaagttccgagacgttattgttgtcaaagctggag aggtccttaagataaatgcagacattgcagggcgacctctgccagtaatttcctgggccaaggatggtatagaaattgaagaaagagcaagaac agaaatcatctcaacagacaatcatactttgttaacagttaaagactgtataagacgagacactgggcaatatgtactaacactgaagaatgttgcc ggcactcggtctgtggccgttaattgcaaagtacttgataagcctggtccaccagcaggaccacttgaaataaatggcctcactgctgagaaatg ctctctttcctggggacgtccccaagaagatggtggtgcagatatcgactattacatcgtagaaaaacgtgaaacaagccaccttgcatggacaa tatgtgaaggagagttacagatgacatcctgtaaagtaaccaagttactcaaaggcaatgaatatatatttagagtaactggtgttaataaatatggt gttggtgagcccctagagagtgtagctataaaggcactagatccatttacagttccaagtccacccacgtctttggaaattacttctgtgaccaaag aatctatgacactttgctggtcaagacccgagagtgatggaggtagtgaaatatctggatatataattgaaaggcgagagaaaaatagcctaaga tgggtgcgtgtaaacaaaaaaccagtttatgatctaagagtgaaatcaacaggacttcgggaaggatgtgaatatgaatatcgtgtttatgcagaa aatgctgctggcctaagtcttccaagtgaaacctctcccttaattagggcagaagatccagtgttcctaccatctcctccatccaaacccaaaattgt ggactcaggcaagacaactataactattgcctgggttaagccgctgtttgatggtggggccccgataactggatatactgtagaatacaaaaaat ctgatgacactgactggaaaacttccattcagagcttacgagggacagaatatacaataagcggactaacaacaggagctgaatatgttttcaga gtaaaatctgtcaataaggttggtgctagtgaGCccagtgatagGtctgaccctcagatagcaaaggaaagagaagaagaacctttatttgatatt gacagtgaaatgaggaagaccttgattgtcaaggctggtgcctcatttaccatgactgtgcctttccgaggaagaccagtacccaatgtcttgtgg agtaagccagacactgacctccgtactagagcttatgttgataccacagactcccgtacatcactgaccattgaaaatgccaacagaaatgactct ggaaagtacacattaacaattcagaatgttttgagtgctgcttcactgaccttagttgtcaaagttttagataccccaggtcctccaaccaacattact gtgcaagatgtaaccaaagagtctgcagtgttatcctgggatgttcctgaaaacgatggtggagcaccagtgaagaattaccacatagaaaaac gtgaggccagcaagaaagcatgggtctctgtgaccaacaactgtaaccgcctctcctacaaagttaccaatttacaagaaggagctatctattact tcagagtctctggagaaaatgagtttggtgttggtataccagctgaaacaaaggaaggagtaaaaataacagaaaaaccaagcccacctgaaa aacttggagtaacaagtatatccaaagacagtgtttccctgacctggctgaagcctgaacatgatggcggaagcagaattgtacactatgtcgttg aagcactagaaaaaggacagaaaaactgggttaaatgtgcagtggcaaagtcaacccatcacgttgtttccggtctgagagagaattctgaata ctttttccgagtgtttgctgaaaatcaagctggcctgagtgacccgagagagcttctgcttcctgttcttattaaggagcaactagaaccacctgaaa ttgatatgaagaatttcccaagtcacactgtatatgttagagctggttcaaaccttaaagttgacattccaatctctggaaaaccacttcccaaagtga ccttatcaagagatggtgtcccccttaaggcaaccatgagatttaataccgaaattactgctgagaacctgaccatcaatctcaaagaaagtgttac agctgacgctgggagatatgaaatcactgctgccaactccagtggtacaaccaaagctttcattaacattgttgtgctagacaggcctggtcctcc aactggccctgttgttattagtgatataactgaagaaagtgtgactctcaaatgggagccacctaagtatgacggtggaagtcaagttacGaacta cattctactcaaaagagaaacaagtactgcagtgtggactgaagtgtctgcaacagttgcaagaaccatgatgaaagtcatgaaactgaccaca ggagaagaataccaattccgcatcaaggcagaaaaccgctttggcatcagtgatcatatagattcagcttgtgtgactgtcaaactaccatacaca acacctggaccaccatctacaccatgggtcactaatgttactcgagaaagcatcactgtgggctggcatgaaccagtgtcaaatggaggcagtg cagtcgtaggctatcacctggaaatgaaagacagaaacagtattttatggcaaaaagccaacaaactggtcatccgcacaactcacttcaaagtc acaacaatcagtgctggacttatttatgaattcagggtgtatgcagaaaatgctgctggagttggaaaacctagccatccttctgaaccagtcttgg caattgatgcttgtgaacccccaagaaatgttcgtatcactgatatttcaaagaactctgtcagcctttcatggcaacaaccagctttcgatggaggt agcaagattacaggctacattgttgagagacgtgaccttccagatggcagatggaccaaggccagcttcaccaatgttactgaaactcaattcat catctctggcttgactcagaattcccagtatgaattccgtgtctttgctaggaatgctgttggttccattagcaatccatctgaggttgtagggcccatt acttgcatcgattcttatggtggtcctgtaattgatttgcctctagaatatacagaagttgtcaaatacagagcaggtacatctgtgaagctcagagct ggcatttctggcaaacctgcgcctactattgagtggtataaagatgataaagaattacaaaccaatgcactggtgtgtgttgaaaataccacggac ctcgcatctatactcatcaaagatgccgatcgccttaatagtggatgctatgaattaaaactaaggaatgccatgggctcagcctcagccaccatc agagtacagatccttgacaaaccaggcccacctggtggaccaattgaatttaagactgtaactgctgagaagatcacccttctctggcggcctcc agctgatgatggtggtgcaaaaatcactcactacattgtggaaaagcgtgagacaagccgcgttgtgtggtctatggtgtctgaacatttggaaga gtgcatcattacaaccaccaaaattatcaaaggaaatgaatacatcttccgggtccgagccgtgaacaaatatggaattggcgagccactggaat ctgattccgttgtagccaagaacgcatttgttacacctgggccaccaggcataccagaagtgacaaagattaccaagaattcgatgactgttgtat ggagcaggccaattgcagatggcggtagtgatataagtggctatttccttgaaaaacgagacaagaagagcctaggatggtttaaagtactaaa agagactatccgtgacaccagacaaaaagtaacaggactcacagaaaacagtgactatcaatacagagtttgtgctgtaaacgctgctggacag ggtccattttctgaaccatctgaattctacaaagctgctgatcctattgatcctccaggtccacctgctaagataagaatcgcagattcaaccaagtc atccatcacccttggctggagtaagcctgtctatgatgggggcagtgctgttactgggtatgttgtcgagataagacaaggagaggaagaggaa tggactactgtctctaccaaaggagaggtcagaactacagaatatgtggtatccaacctgaaacctggagtcaattactacttccgggtatctgct gtaaactgtgctggacaaggagaacctatagaaatgaatgaacctgtacaagctaaagatatacttgaggcaccagagattgacctggatgtgg ctctcagaacttctgttattgccaaagctggtgaagatgtacaagtgttgattccctttaaaggcagacctccacctactgtcacatggagaaaaga tgagaagaatcttggcagtgatgccagatacagcattgaaaacactgattcatcctcattactcaccattcctcaagttactcgcaatgatacagga aaatatattctcacaatagaaaatggagttggtgaacctaagtcttcaactgtgagtgttaaagtgcttgacacaccagctgcctgccagaaactac aggttaaacatgtttctcgaggcacagtcactttgctctgggatcctcctctcattgatggaggatctccaataattaattatgtcattgaaaagagag atgccaccaagagaacatggtctgtcgtgtcacacaaatgttctagcacatccttcaagctaatagatttgtcggagaagactccattcttcttcaga gttcttgcagaaaatgaaattggaattggggaaccctgtgaaactacagagccagtgaaggctgctgaagtaccagctcctatacgtgatctctc aatgaaagactcaacaaagacatctgtcatcctcagctggaccaaacctgactttgatggtggtagcgtcatcacagaatatgttgtagaaagga aaggtaaaggtgaacagacgtggtcccacgctggcataagtaagacatgtgaaattgaggttagccaacttaaggagcagtcagtcctggagtt cagagtgtttgccaaaaatgagaaaggactgagtgatcctgtcactattgggccaattacagtgaaagaacttattattacacctgaagttgacctg tcagatatccctggggcacaagtcactgtgagaattgggcacaatgtgcaccttgaattaccttataagggaaaacccaaaccatccatcagttg gctgaaagatggcttgccactgaaagaaagtgaatttgttcgcttcagtaaaactgaaaacaaaattactttgagtattaagaatgccaagaagga gcatggaggaaaatacactgttattcttgataatgcagtgtgtagaattgcagtccccattacagtcatcacccttggcccaccatcaaagcccaaa ggacccattcgatttgatgaaatcaaggctgatagtgtcatcctgtcatgggatgtacctgaagataatggaggaggagaaattacttgttacagc atcgagaagcgggaaacttcacaaactaactggaagatggtgtgttcaagtgttgccagaacgactttcaaagttcctaatctagtcaaagatgct gagtaccagtttagagtgagagcagaaaacagatacggagtcagccaaccacttgtctcaagcattattgtggcaaaacaccagttcaggattcc tggtcccccaggaaagccagttatatacaatgtgacttctgatggcatgtcactaacttgggatgctccagtttatgatggtggttcagaagttactg gattccatgttgaaaagaaagaaagaaatagcatcctctggcaaaaagttaatacatcaccaatctctggaagagaatatagagccactggactg gtagaaggtctggattaccaattccgtgtatatgctgaaaattctgctggcctaagctcacctagtgacccaagcaaatttaccttagctgtttctcca gtagacccacctggcactcctgactacattgatgtcacccgggaaaccatcacacttaaatggaacccaccattgcgtgatggaggcagtaaga ttgtgggctatagcattgagaaacggcaaggaaatgaacgctgggtgagatgcaactttactgacgtcagtgaatgtcagtacacagttacagga ctcagtcctggggatcgctatgagttcagaataattgcaagaaatgctgttggaactataagcccgccctcacagtcttctggcattattatgacaa gagatgaaaatgttccaccaatagtagagtttggccctgaatactttgatggtctcattattaagtccggagagagccttagaattaaagctttggta caaggaagaccagtgcctcgagtaacttggttcaaagatggagtggaaatcgaaaagaggatgaatatggaaataaccgacgtacttggatcc accagcctatttgttagagatgctactcgggaccatcgtggtgtatacacagtggaagccaaaaatgcatctggttctgcaaaagcagaaattaaa gtgaaagtacaagatacaccaggaaaagtagttgggccaataagattcaccaatattactggggagaagatgactctgtggtgggatgccccac tcaatgacggttgtgctcccataacccactacatcattgaaaaacgggaaaccagcagacttgcctgggcactaattgaggataaatgtgaagcc caaagttacactgccattaaactaataaacggcaatgaataccaattccgtgtttctgcagttaacaagtttggtgttggcaggccacttgattctgat ccagtggttgctcaaatacaatatactgttcctgatgcccctggcattccagaacctagcaacataacaggcaacagcattaccctgacatgggc aaggccagaatcagatggtggcagtgaaattcaacagtatatccttgaaagaagagaaaagaaaagcacaagatgggtaaaagtgatcagca aacgaccaatctctgaaacaagattcaaagtcactggtctgacagaaggcaatgagtatgaattccatgtcatggctgaaaatgctgcaggagtt ggacctgcaagtggcatctcaagactcattaaatgtagagagcccgtcaacccaccaggtcctcccacagtggtcaaagtaacagacacatca aagacaactgtgagcttagaatggtccaaaccagtgtttgatggtggcatggaaataattgggtatattattgaaatgtgtaaggccgacttagga gactggcacaaggtgaatgcagaggcatgtgtgaaaacaagatatacagtcactgatctacaagcaggtgaagaatacaaattccgagttagtg ctatcaatggtgctggaaaaggcgacagctgtgaagtgactggcacaattaaagcagttgaccggttaacagctcctgagttagacatagatgc aaacttcaaacagactcatgttgttagagctggggccagtattcgcctcttcattgcctaccaaggtagacctactcctacagctgtgtggagcaaa ccagactctaaccttagccttcgggctgatatccatacaacagattccttcagcaccctcactgtggaaaactgcaacagaaatgatgcagggaa atatacccttactgtggaaaacaacagtggtagtaagtcaatcacattcaccgtgaaagtgctagacactccaggcccacctggcccaattacctt caaagatgtgacccggggatctgctacattgatgtgggatgcccctcttcttgacggtggtgcccgaatccatcattatgtggtagagaaacgag aggcaagtcgccgtagttggcaggttatcagtgaaaaatgcactcgtcagatcttcaaggtcaatgacctggccgaaggtgttccgtactatttcc gtgtttctgcagtaaatgagtatggtgttggtgagccctatgaaatgccagaaccaattgtagccacagaacagcctgctccacctaggagacttg atgttgttgatactagcaaatcctccgcagtcttagcttggcttaaacctgaccacgatggaggcagccggatcactggctacctgcttgaaatga gacaaaagggatctgacttctgggttgaagctggtcacaccaaacagctaactttcacagtagagcgtcttgttgagaaaactgaatatgaattcc gtgtgaaggccaagaatgatgctggctatagtgaacccagagaagccttctcttctgtcatcattaaggagcctcaaatcgagcccactgctgac ctcactggaattaccaatcagcttataacttgcaaagcaggaagcccatttaccattgacgtaccaatcagtggtcgtcctgcccccaaagtaaca tggaaactggaagaaatgagacttaaagagacagatcgagtgagcattacaacaacaaaagacagaaccacactgactgtaaaggacagcat gagaggtgactctggaagatacttcttgaccctggaaaatacagctggtgttaaaacatttagcgtcacagttgtggtcattggaaggccaggtcc agtaaccggccccattgaggtctcatctgtctcagctgaatcgtgtgtcctgtcatggggagaacctaaagatggaggaggcactgaaattacta attacatagttgaaaagcgtgaatcgggtacaacagcttggcagcttgtcaattccagtgtcaagcgcactcaaattaaagtcactcatctcacaaa atacatggaatattctttccgtgtcagttcagagaacagatttggtgtcagcaaacctctagaatcagcaccaataattgctgaacatccatttgtccc accaagcgctcctaccagacctgaggtctaccatgtgtctgccaatgccatgtctattcgttgggaagaaccctaccacgatggtggcagtaaaa tcattggctactgggttgagaagaaagaacgtaatacaattctttgggtgaaagaaaacaaagtgccatgcttagagtgcaactacaaagtaactg gtttagtagaaggactggaatatcagttcagaacttatgcactcaatgctgcaggtgttagcaaggccagcgaagcttcaagacctataatggctc aaaatccagttgatgcaccaggcagaccagaggtgacagatgtcacaagatcaacagtatcactgatttggtctgccccagcgtatgatggagg cagcaaggttgtgggctacatcatagagcgtaagccagtcagtgaggtaggagatggtcgctggctgaagtgcaactacaccattgtatctgac aatttcttcaccgtgactgctctcagtgaaggagacacttatgagttcGgtgtgttagccaagaatgcagcaggcgtaattagcaaagggtctgaa tctacaggccctgtcacttgccgagatgaatacgctccacccaaagccgaactggatgcccgattacacggtgatctggttaccatcagagcag gttctgatcttgttctggatgctgcagttggtggcaaacctgaacccaaaattatctggaccaaaggagacaaggagctagatctctgtgaaaaag tctctttgcagtatactggcaaacgagcaactgctgtgatcaagttctgtgacagaagtgacagtggaaaatacactttaacagtgaaaaatgcca gcgggaccaaggccgtgtctgtcatggtcaaagtgcttgattcccctggcccatgtggaaagctcaccgtcagcagagtaacacaggagaagt gcactttagcctggagccttccgcaggaagacggaggagcagaaatcactcactacatcgtggaaagacgcgagactagcaggctcaactgg gtgattgttgaaggcgaatgcccaaccctatcctatgtcgttaccaggctcatcaagaacaatgagtacatattccgagtgagggcagtaaacaa atatggccctggtgtgcctgttgaatcagagccaattgtagccagaaactcattcactattccatcaccacccggcatacctgaagaagttgggac tggcaaagagcatatcatcattcagtggacaaaacctgaatctgatggtggcaatgaaatcagcaactacctagtagacaaacgtgagaagaag agcctgcgctggacacgtgtcaacaaagactatgtggtgtatgataccaggctgaaggtgaccagcctgatggagggttgtgattaccagttcc gggtgaccgcagtgaatgcagctggtaacagtgagcccagcgaagcttccaacttcatctcatgcagagaaccatcatatacccctggaccac cttctgctccaagagttgtggataccaccaaacacagcattagtttggcatggaccaaacccatgtacgatggtggtactgacattgtaggatatgt tctggaaatgcaagagaaggacactgatcagtggtaccgagtgcataccaatgccacaataagaaatactgaattcactgtgccagaccttaaa atgggccagaaatattccttcagagttgctgccgtgaacgtgaagggtatgagcgaatacagcgaatcaattgctgaaattgagcccgtggaaa gaatagaaataccagatcttgagcttgcagatgatctaaagaagactgtgaccatcagggctggggcctccttgcgcttgatggtgtctgtatctg gaagaccacctcctgtcataacgtggagcaagcagggcattgaccttgcaagccgggcaattattgacaccactgagagctactcattgctaata gtggacaaagttaatcggtacgatgctggaaaatacacaattgaagctgaaaaccaatctggcaagaaatcagcaacagtccttgttaaagtctat gatactcctggtccctgtccttcagtgaaagttaaggaagtatcaagagattctgtgactataacttgggaaattcccacgattgatggtggagctc cagtcaacaattacatcgttgagaagcgtgaagctgctatgagagcattcaaaacagtaactaccaaatgcagcaagacactttacagaatttctg gacttgtagaaggaaccatgtactatttcagagtgctgccagaaaatatttatggcattggagaaccttgtgaaacatctgatgcagtactggtctc agaagtgcctttggtgcctgcaaagctagaagtggtcgatgtcaccaaatccactgttacccttgcctgggaaaaaccactctacgatggtggta gccgactcactggatatgttctcgaggcctgcaaagctggcacagagagatggatgaaggttgtcaccttaaaacccacagtcctagagcacac tgttacttccttaaatgaaggtgaacaatacttatttagaataagggcacaaaatgagaaaggtgtgtcagaaccaagagagactgtcacagccgt gactgtacaagacctcagagtgttgccaacaatcgatctttctacaatgcctcagaagaccatccatgtcccagctggcagaccagtagagctgg tgatacctattgctggccgtccacctcctgctgcttcctggttctttgctggttctaaactgagagaatcagagcgtgtcacagttgaaactcacact aaagtagctaaattaaccatccgtgaaaccactatcagagatactggagaatacacacttgaattgaagaatgttaccggaactacttcagaaacc attaaagttatcattcttgacaagcctggtccaccaacaggacctattaagattgatgaaattgatgctacatcaattaccatttcctgggaaccacct gaattggacggtggtgctccactgagtggttatgtggtagaacaacgtgacgctcatcgtccaggatggctgcccgtttctgaatcagtgactag gtccacgtttaagtttaccagactcaccgaaggaaatgagtatgtgttccgtgtggctgcaacaaaccgcttcgggattggctcttacttgcagtct gaggtcatagagtgtcgcagcagcatccgtattcctggacccccagaaacattacagatatttgatgtttcccgtgatggcatgacacttacttggt acccaccagaggatgacggtggctcccaagtgactggatatattgtggagcgcaaagaagtgagagcagatcgatgggtccgtgtaaataaa gtacctgtgacaatgacacggtaccgctccactggccttactgaaggcttagaatatgaacaccgtgtcacagccattaatgcaagagggtctgg gaaaccaagtcgtccttccaaacccatcgttgccatggatccaattgctcctccaggaaagccacaaaacccaagagttactgatacaacaagg acatcagtctccctggcctggagtgttccagaagatgaaggaggatctaaagtcacaggctacttgattgaaatgcaaaaagtagatcaacatga atggaccaagtgtaacaccactccaaccaagattcgagagtatactctaacacacctacctcagggtgcagaatacaggttccgcgtcctagctt gtaatgctggtggacctggtgagcctgctgaggtaccaggaacagtcaaagtcactgaaatgcttgaatatcctgattatgaacttgatgaaagat accaagaaggtatctttgtaaggcaaggtggcgtcatcagacttaccataccaatcaaaggaaaaccattcccaatatgtaaatggaccaaggaa ggccaggatattagtaagcgtgccatgattgcaacatctgaaacacacactgagcttgtgatcaaagaagcagacaggggtgattctggcactta tgacctggttctggaaaataaatgtggcaagaaggctgtctacatcaaggtcagggtgataggaagtcccaacagtccagaagggccactgga atatgatgacatccaagtccgctctgtgagggtcagctggagacctcctgctgatgatggtggtgctgacatcttaggctacatcctcgagagac gagaagtgcctaaagccgcctggtataccattgattccagagtccgaggtacatctctggtggtaaaaggcctcaaagagaatgtagaataccat ttccgtgtttcagcagaaaaccagtttggcataagcaaacccttgaaatctgaggaaccagtcacaccaaaaacaccattgaatcctccagaacc tccaagcaatcctccagaagtactcgatgtaaccaagagttctgttagcttgtcctggtcccggcccaaagatgatggtggttctagagtcacagg ctactacatcgaacgcaaagagacatccactgacaagtgggtcagacacaacaagactcagatcaccaccacaatgtacactgtcacagggct tgttcccgatgctgagtatcagttccgcatcatcgcacagaatgatgttggcctgagtgagaccagccctgcttctgaaccagttgtttgcaaagat ccatttgataaaccaagccaaccaggagaacttgagattctttcaatatccaaagatagtgtcactctacagtgggagaaacctgaatgtgatggt ggtaaagaaattcttggatactgggttgaatatagacagtctggagacagtgcctggaagaagagcaataaggaacgtattaaggacaagcaat tcacaataggaggtttgctggaagctactgagtatgaattcagggtttttgctgagaatgagactgggctgagcagacctcgcagaactgctatgt ctataaagactaaactcacatctggagaggccccaggaatacgcaaagaaatgaaggatgttaccacaaaattgggtgaagctgctcaactctc atgccagattgttggaaggcctcttcctgacattaaatggtacagatttggtaaagagctcatacaaagccggaaatacaaaatgtcttcagatgg acgcacacacactcttacagtaatgacagaggaacaggaagatgaaggtgtttatacctgcatagccaccaatgaggttggagaagtagaaac cagtagtaagcttctcctgcaagcaacaccgcagttccatcctggttacccactgaaagagaaatattatggagctgtgggttccacacttcggctt catgttatgtacattggtcgtccagtacctgccatgacttggttccatggtcagaaacttttgcaaaactcagaaaacattactattgaaaacactga gcactatactcatcttgtcatgaagaatgtccaacgtaagactcatgctgggaaatacaaagtccagctcagcaatgtttttggaacagttgatgcc atccttgatgtggaaatacaagataaaccagacaaacctacaggaccaattgtgatcgaagctctattgaagaactccgcagtgatcagctggaa accacccgcagatgacggaggctcctggatcaccaactatgtggtggaaaaatgtgaggccaaggagggggctgaatggcaattggtgtcttc agccatctcagtgacaacctgtagaattgtgaacctcacagaaaatgctggctattacttccgggtttcagctcagaacactttcggcatcagtgac cctctagaagtgtcctcagttgtgatcattaagagtccatttgaaaagccaggtgctcctggcaaaccaactattactgctgtcacaaaagattcttg tgttgtggcctggaagccacctgccagtgatggaggtgcaaagattagaaattactaccttgagaagcgtgagaagaagcagaataaatggattt ctgtgacaacagaagaaattcgagaaactgtcttttcagtgaaaaaccttattgaaggtcttgaatacgagtttcgtgtgaaatgtgaaaatctaggt ggggaaagtgaatggagtgaaatatcagaacccatcactcccaaatctgatgtcccaattcaggcaccacactttaaagaggaactgagaaatc taaatgtcagatatcagagcaatgctaccttggtctgcaaagtgactggtcatccaaaacctatcgtcaaatggtacagacaaggcaaagaaatc attgcagatggattaaaatataggattcaagaatttaagggtggctaccaccagctcatcattgcaagtgtcacagatgatgatgccacagtttacc aagtcagagctaccaaccaagggggatctgtgtctggcactgcctccttggaagtggaagttccagctaagatacacttacctaaaactcttgaa ggcatgggagcagttcatgctctccgaggtgaagtggtcagcatcaagattcctttcagtggcaaaccagatcctgtgatcacctggcagaaag gacaagatctcattgacaataatggccactaccaagttattgtcacaagatccttcacatcacttgttttccccaatggggtagagagaaaagatgc tggtttctatgtggtctgtgctaaaaacagatttggaattgatcagaagacagttgaactggatgtggctgatgttcctgacccacccagaggagtc aaagttagtgatgtctcacgagattctgtcaacttaacatggactgagccagcctctgatggtggcagcaaaatcaccaactacattgttgaaaaat gtgcaactactgcagaaagatggctccgtgtaggacaggcccgagaaacacgttataccgtgatcaacttatttggaaaaacaagttaccagttc cgggtaatagctgaaaataaatttggtctgagcaagccttcagagccttcagaaccaaccataaccaaagaagataagaccagagctatgaact atgatgaagaggtagatgaaaccagggaagtctccatgactaaagcatctcactcttcaaccaaggaactctatgagaaatatatgattgctgaa gatcttgggcgtggtgagtttggaattgtccatcgttgtgttgaaacatcctcaaagaagacatacatggccaaatttgttaaagtcaaagggactg atcaggttttggtaaagaaggaaatttccattctgaatattgctaggcatagaaacatcttacacctccatgaatcatttgaaagcatggaagaatta gttatgatctttgagtttatatcaggacttgacatatttgagcgcattaacacaagtgcttttgaacttaatgaaagagaaattgtaagttatgttcacca ggtctgtgaagcacttcagtttttacacagtcataatattggacactttgacattagaccagaaaatatcatttaccaaaccagaagaagctctacca ttaaaatcatagaatttggtcaagcccgtcagctgaaaccaggggacaacttcaggcttctattcactgccccagaatactatgcacctgaagtcc accagcatgatgttgtcagcacagccacagacatgtggtcacttggaacactggtatatgtgctattgagtggtatcaacccattcctggctgaaa ctaaccaacagatcattgagaatatcatgaatgctgaatatactttcgatgaggaagcattcaaagagattagcattgaagccatggattttgttgac cggttgttagtgaaagagaggaaatctcgcatgacagcatcggaggctctccagcacccatggttgaagcagaagatagaaagagtcagtact aaagttatcagaacattaaaacaccggcgttattaccacaccctgatcaagaaagacctcaacatggttgtgtcagcagcccggatctcctgtggt ggtgcaattcgatctcagaagggagtgagtgttgctaaagttaaagtggcatccattgaaattggcccagtttctgggcagataatgcatgcagtt ggtgaagaaggaggacatgtcaaatatgtatgcaaaattgaaaattatgatcagtctacccaagtgacttggtactttggcgtccgacagctggag aacagtgagaaatacgaaatcacctacgaagatggagtggccatcctctatgtcaaagacattaccaaattagatgatggtacctacagatgcaa agtagtcaatgactatggtgaagacagttcttatgcagagctatttgttaaaggtgtgagagaagtctatgactattactgccgtagaaccatgaag aaaattaagcgcagaacagacacaatgagactcctggaaaggccaccagaatttaccctgcctctctataataagacagcttatgtaggtgaaaa tgtccggtttggagtaactataactgtccacccagagcctcatgtaacatggtataaatcaggtcagaaaatcaaaccaggtgacaatgacaaga agtacacatttgagtcagacaagggtctttaccaattaacaatcaacagtgtcactacagatgatgacgctgaatatactgttgtggcaaggaaca aatatggtgaagacagctgtaaagcaaagctgacagtaaccctacacccacctccaacagatagtaccttaagacccatgttcaaaaggttactg gcaaatgcagaatgccaagaaggccaaagtgtctgctttgagatcagagtgtctggcatccccccaccaacattaaaatgggagaaagatggtc agccactgtccctcgggcctaacattgaaattatccatgaaggcttggattattatgctctgcacatcagggacactttgcctgaagacacgggtta ttatagagtcacagccactaacacagctgggtccaccagctgccaggctcacctacaagtggaacgcctgaggtacaagaaacaggaattcaa gagtaaggaggagcatgagcgacacgtacaaaaacaaattgacaaaaccctcagaatggctgaaattctttctggaactgaaagtgtaccactg acacaggtagctaaagaggctctgagagaagctgctgtcctttataaaccggctgtaagcaccaagactgtaaaaggggaattcagacttgaga tagaagaaaagaaggaggagagaaaactccggatgccttatgatgtaccagagccacgcaagtataagcagactaccatagaagaagacca acgcatcaagcagttcgtgcccatgtctgacatgaagtggtataaaaagatacgtgatcagtatgaaatgcctgggaaacttgacagagttgtac agaaacgacccaagcgcatccgcctttcaagatgggaacagttctatgtgatgcctcttccacgcattacagatcaatacagacctaaatggcgt attcctaaactgtcccaagatgatcttgagatagtgagaccagcccgccggcgtacaccttctcctgattatgacttttactaccgacctagaagac gttctcttggggacatctctgatgaagaattactcctccccattgatgactacttagcaatgaaaagaacagaggaagagaggctgcgtcttgaag aagagcttgagttaggtttttcagcttcacccccaagtcgaagccctccacactttgagctttctagcctacgttactcttcaccacaagctcatgtca aggtggaggaaacaagaaaagacttcaggtattcaacctatcacatcccaacgaaggctgaagctagtacaagttatgcagaactgagggaac ggcatgcccaggctgcgtacagacagccaaagcaacggcaaagaatcatggctgagagggaggatgaagagttgcttcgcccagttacgac cacccagcatctctcagaatacaaaagcgaacttgacttcatgtcaaaggaggaaaagtctagaaagaaatcaaggcgacaaagagaagtgac agaaataacagaaattgaggaagaatacgaaatctcaaaacatgctcaaagagaatcatcctcatctgcgtctagactactgagacgacggcgc tccctgtctccaacttatattgagttaatgaggccagtgtctgagctgatccggtcacgtccacaaccggctgaggaatacgaagatgacacaga aagaaggtcacctactccagagagaactcgcccacgatcccccagccctgtgtctagtgagagatcactctcgagatttgagaggtctgcaaga tttgatatcttttccaggtatgagtccatgaaagctgctttaaaaactcagaagacatcagaaaggaagtatgaagttttgagtcagcagcctttcac actggaccatgcccctcgaatcacactgagaatgcgctcgcacagggtaccatgtggccaaaatacacgttttattttaaatgttcagtctaagcc aactgccgaggttaaatggtaccacaatggtgtggaactccaagaaagcagtaagattcattacaccaacacgagtggagtcctcaccctggaa attctggactgtcatactgatgacagtggaacctaccgtgctgtgtgcaccaactacaagggcgaagcttctgactatgcaacgttggacgtgac aggaggggattataccacctatgcttcccaacgcagagatgaagaggtccccagatctgttttccctgagctgacaagaacagaggcgtatgct gtttcatcatttaagaaaacatctgagatggaagcttcgtcttctgtcagggaagtgaaatcacagatgacggagacaagggaaagtctctcctca tatgaacactctgcatctgcagaaatgaaaagtgctgcattagaagaaaagtcactggaagaaaaatccacaaccagaaagatcaagacgactt tggcagcaagaattctaacaaagccacggtccatgaccgtctacgagggcgagtctgcaaggttttcttgtgacaccgatggtgagccggtacc aactgtgacctggctgcgtaaaggacaagtgctaagtacttctgcccgccaccaagtgaccaccacaaagtacaaatcaacctttgagatctctt cagtccaggcttccgatgagggcaattacagcgtggtggtagaaaacagtgaagggaaacaagaagcagagttcactctgactattcaaaagg ccagggtaactgaaaaggctgtgacatcaccaccaagagtcaaatccccagagcctcgggtgaaatccccagaagcagttaagtctccaaaac gagtgaaatctccagaaccttctcacccgaaagccgtatcacccacagagacaaaaccaacaccaacagagaaagttcagcacctcccagtct ctgccccaccaaagattactcagttcctgaaagcagaagcttctaaagagattgcaaaactgacctgtgtggttgaaagcagtgtattaagggca aaagaggtcacctggtataaagatggcaagaaactgaaggaaaatgggcatttccagtttcattattcagcagatggtacctatgagctcaaaatc aataacctcactgaatctgatcaaggagaatatgtttgtgagatttctggtgaaggtggaacgtctaaaaccaacttacaatttatggggcaagcctt taagagtatccatgagaaggtatcaaaaatatcagaaactaagaaatcagatcagaaaaccactgagtcaacagtaaccagaaaaactgaacca aaagctcctgaaccaatttcctcaaaaccagtaattgttactgggttgcaggatacaactgtttcttcagacagtgttgctaaatttgcagttaaggct actggagaaccccggccaactgccatctggacaaaagatggaaaggccattacacaaggaggtaaatataaactctctgaagacaagggagg gttcttcttagaaattcataagactgatacttctgacagtggactttatacttgtacagtaaaaaattcagctggatctgtgtcctctagctgcaaattaa caataaaagctataaaagatactgaggcacagaaagtctctacacaaaagacttctgaaattacacctcagaagaaagctgttgtccaagagga aatttcccaaaaagccctaaggtctgaagaaattaagatgtcagaggcaaaatctcaagaaaagttagccctcaaagaggaagcttcaaaggttc tgatttctgaagaagtcaagaaatcagcagcaacctccctggaaaaatccattgtccatgaggaaatcactaaaacatcacaggcatcagaaga agtcagaactcatgctgagattaaagcattttctactcagatgagcataaacgaaggtcaaagactggttttaaaagccaacattgctggtgccact gatgtgaaatgggtactgaatggcgtagagcttaccaactctgaggagtaccgatatggtgtctcaggcagcgatcagaccctaaccatcaagc aagccagtcacagagatgaaggaatcctcacctgcataagcaaaaccaaggaaggaatcgtcaagtgtcagtatgatttgacactgagcaaag aactctcagatgctccagccttcatctcacagcctagatctcaaaatattaatgaaggacaaaatgttctctttacttgtgaaatcagtggcgagcca tcccctgaaatcgaatggtttaaaaacaacctgccaatttctatttcttcaaatgtcagcataagccgctccagaaatgtatactcccttgaaatccga aatgcatcagtcagcgacagtggaaagtacacaattaaggccaaaaatttccgtggccagtgttcagctacagcttccttaatggtccttcctctag ttgaagaaccttccagagaggtagtattgagaacaagtggtgacacaagcttgcaaggaagcttctcgtctcagtcagtccaaatgtctgcctcca agcaggaggcctccttcagcagtttcagcagcagcagtgctagcagcatgactgagatgaaatttgcaagcatgtctgcccaaagcatgtcctc catgcaagagtcctttgtagaaatgagttccagcagctttatgggaatatctaatatgacacaactggaaagctcaactagtaaaatgcttaaagca ggcataagaggaattccgcctaaaattgaagctcttccatctgatatcagcattgatgaaggcaaagttctaacagtagcctgtgctttcacgggtg agcctaccccagaagtaacatggtcctgtggtggaagaaaaatccacagtcaagaacaggggaggttccacattgaaaacacagatgacctga caaccctgatcatcatggacgtacagaaacaagatggtggactttataccctgagtttagggaatgaatttggatctgactctgccactgtgaatat acatattcgatccatttaagagggcctgtgcccttatactctacactcattcttaacttttcgcaaacgtttcacacggactaatctttctgaactgtaaa tatttaaagaaaaaaaagtagiittgtatcaacctaaatgagtcaaagttcaaaaatattcatttcaatcttttcataattgttgacctaagaatataatac atttgctagtgacatgtacatactgtatatagccggattaacggitataaagttttgtaccatttattttatgacattttacaatgtaagttttgaaactaart gttggtaggagaaagtttcttatggaacgaataccctgctcaacatttaatcaatctttgtgcctcaacatactgttgatgtctaagtatgcctcagtgg gttgagaaaatccccattgaagatgtcctgtccacctaaaagagaatgatgctgtgcatatcacttgatatgtgcaccaatacctactgaatcagaa atgtaaggcattggtgatgtttgcatttaccctcctgtaagcaacactttaacgtcttacattttctctgatgatgtcacacaaaattatcatgacaaata ttaccagagcaaagtgtaacggccaacactttgttcgctcattttacgctgtctctgacataaggagtgcctgaatagcttggaaaagtaacatctc ctggccatcccttcatttaaccaagctattcaagtattcctatgccagagcagtgccaactcttggaggtcccagagtgcagccaatgcctttgtgt ggtagttctaaattttaattgcacctgaaaaacctgggcacctaagcaatgagccacagcaaaaagtaaagaacaacaacaaaataaagctgttg ttaaattttaaacaatattactaattgcccaaaatgtcaatttgatgtagttcttttcatgcaagtataaattcaattgttagttataattgttggacctccttg agatagtaacaacaaaataaagcaagctatctgcacctcaaaa Additional isoforms exist; namely, isoform N2B (NM_003319); isoform novex-1 (NM_133432); isoform novex-2 (NMJ33437); isoform novex-3 (NMJ33379).
[0387] Following is a LOCI 12609 cDNA sequence (SEQ ID NO: 13). NMJ38409 - LOC112609 tccggcgccccgcgccgcctccgctgcgggtcgggagcgcgcgtctccgccgcacctcggatctaggagctactcgcccggccctgggcg gtgggaggcggcggcggcggcggcgctcgcgcacctcggaggagccaggagccggaaccagggccgagcccgcgggccggggctag ccagccggtcggagatgtccgcccagaggttaatttctaacagaacctcccagcaatcggcatctaattctgattacacctgggaatatgaatatt atgagattggaccagtttcctttgaaggactgaaggctcataaatattccattgtgattggaiittgggttggtcttgcagtcttcgtgatttttatgtttat tgtgctgaccttgctgaccaagacaggagccccacaccaagacaatgcagagtcctcagagaagagattcagaatgaacagctttgtgtcaga ctttggaagacctctggagccagataaagtattttctcgccaaggcaacgaggagtccaggtctctctttcactgctacatcaatgaggtggaacg cttggacagagccaaagcttgtcaccagaccacagcccttgacagtgacgtccaactccaggaagccatcagaagcagtgggcagccagag . gaggagctgaacaggctcatgaagtttgacatccccaactttgtgaacacagaccagaactactttggggaggatgatcttctgatttctgaacca cctattgttctggaaactaagccactttcccagacctcacacaaagacctggattgagaaacatgctctgtaaagggtcttcctgaagatgtggatt ctatctttatgtagcaagaaatctacatccaccaaaattgtgtgtgtttgggggagagagagacatagagatagagacagagaggcagagaaga gacccctttagaagagagctgagctgattaagctgagtggttttttgttttgtttt^ acaccaagcttgtccaacccgtggcatgtgtcccaggacagctttgaatgtggcccaatacaaattttaaactttattaaaacatgagttttgtttttttt tttgctattttttttaaagctcgtcagctatcgttagtgttagtgtactttatgtgtggcccaagacaactcttcttccagtgtggcacagggaagctaaa agattggacacctctgatttatactagctcgttttgcttgttgaaaaatttggccaaatacctattgtcagcattcttgggtgaggattagcctaccatgt tctaatctggccctgccactactatgctctøcctttggtgagttgctttacctctctgggctgccccatttttaactgtaggttgacaggtctagagtgat ccatcccacctctaatattttgtgaatttatgactttgccttcagatgaggctgagctatacataaaacagtataaactagggtactgcctcgtatctctt gtaggctctctcaaatctctgtaccttccacttaaccctaattgagccaagctttagtcaggggatctggttgtctaccagaatgtcaggagactcat cttacacagtcatggtggccaatgtttctggtgggttgtgctgaaacagctcttctgagaacttccaaccacccatgctctaacctggagacagcc atcccctgcctcagaataagtaccaattcgtagtacatgtatggtactcttgtccccaagaaatgttaggaagcttgtcagctgaatgagaggaggt gccttctgggtatctctgtgttggtgtatctgtgccattggctacagaacaagaaaaatactatttgccatgctattaccttggcagatgtgtaggtga tagtcatctggctttgagctgagatggtcagtgggttgtaaattccccactagcagatattcagggtggcctgagttatgtaaacaagtgagcaaca cagctttaatttcatggaggaatcaaagctgcacactggtattaaaacaacttgattttgcgcacacagttgcatgcatggcaagctgttaacctctg ggtggcattttcattatgaatttgttcaccacctgtcttgcttaagctacaaaataaatgcatttgactgc
[0388] Following is a TRPSl cDNA sequence (SEQ ID NO: 14). NM_014112 - TRPSl ttcctccgcgaaggctcctttgatattaatagtgttggtgtcttgaaactgacgtaatgcgcggagactgaggtcctgacaagcgataacatttctga taaagacccgatcttactgcaatctctagcgtcctcttttttggtgctgctggtttctccagacctcgcgtcctctcgattgctctctcgccttcctatttc llllLtUULLUaaacaaaaaacaacaccccctcccctctcccacccggcaccgggcacatccttgctctatttcctttctctttctctctctctctctctc ttttttaataagggtgggggagggaaaggggggggaggcaggaaagacctttttctctcccccccgcaataatccaagatcaactctgcaaaca acagaagacggttcatggctttggccgccgcgccaccatctttcgggctgccgagggtgttcttgacgattaatoaacagatgtacagatcagct ctcaaaatgtcttctgtgtcttctgagcgtcttctaagacaattgcattagcctcctgctagttgactaatagaattaataattgtaaaaagcactctaaa gccacatgccttatgaagtcaatgctgggtatgattttacaaatatggtccggaaaaagaacccccctctgagaaacgttgcaagtgaaggcgag ggccagatcctggagcctataggtacagaaagcaaggtatctggaaagaacaaagaattctctgcagatcagatgtcagaaaatacggatcag agtgatgctgcagaactaaatcataaggaggaacatagcttgcatgttcaagatccatcttctagcagtaagaaggacttgaaaagcgcagttctg agtgagaaggctggcttcaattatgaaagccccagtaagggaggaaactttccctcctttccgcatgatgaggtgacagacagaaatatgttggc tttctcatttccagctgctgggggagtctgtgagcccttgaagtctccgcaaagagcagaggcagatgaccctcaagatatggcctgcaccccct caggggactcactggagacaaaggaagatcagaagatgtcaccaaaggctacagaggaaacagggcaagcacagagtggtcaagccaatt gtcaaggtttgagcccagtttcagtggcctcaaaaaacccacaagtgccttcagatgggggtgtaagactgaataaatccaaaactgacttactg gtgaatgacaacccagacccggcacctctgtctccagagcttcaggactttaaatgcaatatctgtggatatggttactacggcaacgaccccac agatctgattaagcacttccgaaagtatcacttaggactgcataaccgcaccaggcaagatgctgagctggacagcaaaatcttggcccttcata acatggtgcagttcagccattccaaagacttccagaaggtcaaccgttctgtgttttctggtgtgctgcaggacatcaattcttcaaggcctgttttac taaatgggacctatgatgtgcaggtgacttcaggtggaacattcattggcattggacggaaaacaccagattgccaagggaacaccaagtatttc cgctgtaaattctgcaatttcacttatatgggcaactcatccaccgaattagaacaacattttcttcagactcacccaaacaaaataaaagcttctctc ccctcctctgaggttgcaaaaccttcagagaaaaactctaacaagtccatccctgcacttcaatccagtgattctggagacttgggaaaatggcag gacaagataacagtcaaagcaggagatgacactcctgttgggtactcagtgcccataaagcccctcgattcctctagacaaaatggtacagagg ccaccagttactactggtgtaaattttgtagtttcagctgtgagtcatctagctcacttaaactgctagaacattatggcaagcagcacggagcagtg cagtcaggcggccttaatccagagttaaatgataagctttccaggggctctgtcattaatcagaatgatctagccaaaagttcagaaggagagac aatgaccaagacagacaagagctcgagtggggctaaaaagaaggacttctccagcaagggagccgaggataatatggtaacgagctataatt gtcagttctgtgacttccgatattccaaaagccatggccctgatgtaattgtagtggggccacttctccgtcattatcaacagctccataacattcac aagtgtaccattaaacactgtccattctgtcccagaggactttgcagcccagaaaagcaccttggagaaattacttatccgtttgcttgtagaaaaa gtaattgttcccactgtgcactcttgcttctgcacttgtctcctggggcggctggaagctcgcgagtcaaacatcagtgccatcagtgttcattcacc acccctgacgtagatgtactcctctttcactatgaaagtgtgcatgagtcccaagcatcggatgtcaaacaagaagcaaatcacctgcaaggatc ggatgggcagcagtctgtcaaggaaagcaaagaacactcatgtaccaaatgtgattttattacccaagtggaagaagagatttcccgacactaca ggagagcacacagctgctacaaatgccgtcagtgcagttttacagctgccgatactcagtcactactggagcacttcaacactgttcactgccag gaacaggacatcactacagccaacggcgaagaggacggtcatgccatatccaccatcaaagaggagcccaaaattgacttcagggtctacaa tctgctaactccagactctaaaatgggagagccagtttctgagagtgtggtgaagagagagaagctggaagagaaggacgggctcaaagaga aagtttggaccgagagttccagtgatgaccttcgcaatgtgacttggagaggggcagacatcctgcgggggagtccgtcatacacccaagcaa gcctggggctgctgacgcctgtgtctggcacccaagagcagacaaagactctaagggatagtcccaatgtggaggccgcccatctggcgcga cctatttatggcttggctgtggaaaccaagggattcctgcagggggcgccagctggcggagagaagtctggggccctcccccagcagtatcct gcatcgggagaaaacaagtccaaggatgaatcccagtccctgttacggaggcgtagaggctccggtgttttttgtgccaattgcctgaccacaa agacctctctctggcgaaagaatgcaaatggcggatatgtatgcaacgcgtgtggcctctaccagaagcttcactcgactcccaggcctttaaac atcattaaacaaaacaacggtgagcagattattaggaggagaacaagaaagcgccttaacccagaggcacttcaggctgagcagctcaacaa acagcagaggggcagcaatgaggagcaagtcaatggaagcccgttagagaggaggtcagaagatcatctaactgaaagtcaccagagaga aattccactccccagcctaagtaaatacgaagcccagggttcattgactaaaagccattctgctcagcagccagtcctggtcagccaaactctgg atattcacaaaaggatgcaacctttgcacattcagataaaaagtcctcaggaaagtactggagatccaggaaatagttcatccgtatctgaaggga aaggaagttctgagagaggcagtcctatagaaaagtacatgagacctgcgaaacacccaaattattcaccaccaggcagccctattgaaaagta ccagtacccactttttggacttccctttgtacataatgacttccagagtgaagctgattggctgcggttctggagtaaatataagctctccgttcctgg gaatccgcactacttgagtcacgtgcctggcctaccaaatccttgccaaaactatgtgccttatcccaccttcaatctgcctcctcatttttcagctgtt ggatcagacaatgacattcctctagatttggcgatcaagcattccagacctgggccaactgcaaacggtgcctccaaggagaaaacgaaggca ccaccaaatgtaaaaaatgaaggtcccttgaatgtagtaaaaacagagaaagttgatagaagtactcaagatgaactttcaacaaaatgtgtgca ctgtggcattgtctttctggatgaagtgatgtatgctttgcatatgagttgccatggtgacagtggacctttccagtgcagcatatgccagcatctttg cacggacaaatatgacttcacaacacatatccagaggggcctgcataggaacaatgcacaagtggaaaaaaatggaaaacctaaagagtaaa accttagcacttagcacaattaaatagaaataggttttcttgatgggaattcaatagcttgtaatgtcttatgaagacctattaaaaaaatacttcatag agcctgccttatccaacatgaaattcccttcttttgttattctttcttttgatgagtaggttaccaagattaaaaagtgagataaatggtcaatgagaaag aatggaagatggtaaacaatcactttttaaaacctgttaagtcaaaaccatcttggctaatatgtactggggaaataatccataagagatatcacca gactagaattaatatatttataaagaaagagaccaaaactgtctagaatttgaaagggtttacatattattatactaaagcagtactggactggccatt ggaccatttgttccaaaacccataaattgttgcctaaatttataatgatcatgaaaccctaggcagaggaggagaaattgaaggtccagggcaatg aaagaaaaatggcgccctctcaatttagtcttctctcattggccatgtttcagattttgacctagaaatgcgagctgtggttaggcttggttagagtgc agcaagcaacatgacagatggtggcacgctgtttttacccagccctgcctgtacatacacatgcacaccctctctgatatttttgtcctttagatgttc aaatactcagtagtccttttgtttgcggtttagattcattttgtccacaca^ agccaggtatttctttcttgtgtgtgatgaaccagtatggatttgcttttctaagcctcctgttggttactaatctcacttggcacattataactaaaggaa tcccctcaattcaaaagcatagatggatacaaatgtcagaccgtgggtttaatttgtttagaacacatggcatttcttcacaaggtaacctgctgtattt atttattttcttttggttaaatataatttccaaactttgtggtcaggcagcgtctaaggttacgttaccacagactgacagttggtatatgtaccagccaa tcccttcattaaatgtatacagatttagttaagtagcattaaataggattcttagaagtatgtcctcatagaacttttaatacttaaggctttgtaaaaact atccatgaagggaaagctcctcagcataactgctcagggaaatagggctaaataactgaacattaaataattggttaaaggtgctgttagtcgagc ctcaatgcttgctacaaggatgtatgtacaaggactgactttaataatttgcattatattgtcccaaccagtagtttattttttgccacggagatgtagaa gatattacaagctactggatgcactg^cagattaacttatttcattaaagaagttgggagaacaaataggaaaaaaaaaacttatttttctagtaaata ttaatgtattacatttcaaataatggtgcctgacatattgaataattattttctacagtgtacgtatgcaacaaagatattccatcatgcattagagtcagt tctggctctgcctagctgtttacatttgcaaatgtagcaaacaaggtaatgaagcaactatttctattgcagtagatatccttttgtgtgtgtgtgtgtgc attaaagttgtaaacggtaacatgaaacaaatgaaagttcttgctataatggtatggaaaacaagaaggaaatgaaaatatttttatgcctacttagg aaaaaaagggtagcacttattcattccaagtactllUUtLLUaatttttaagctcttaactcacattgttatgcttaagatgataaacatatatcctcttttt attgctttgtctatgtttcatatgaaacatttcagaaattattttgataagtgttgctggaatctgcaacgctgatttttttttgcattctgtagtcgcat^ actccatttttacattaattcgcagttgciitgtatcattgttttgtttgggttttgtttctttttcacagtgccggg^ctt^ tagagttcaaccagftcgtgactgftgtagcgaatgaagttaaaaaaatgfctttctgatg^ aaaaaagagaaaagagaaagcaagagacagaaatcaggactaagtcctctgcttcagtttcattgttaacgggccttattctgatctcacctgtcg cgtagctctaatattcacataaactgaaataaagaagtggaatgaggagctttgacattcaaattatgtgatgtaatttatcttccttaggaattttgatg gatgcatctcaaaatgtatagccagacttgagaggtgacaattaaagatctaaaaaagagaggagattcccccaaacaacaatatttaattttctta gtaaaaagaataacagaatgcatcgtggcaatccttaagcaacattatctatgtggactgcttaaatcagcaaaacaccagaagtttggttaacttg ggcaatatgacaagtattactttttgggcaaaactactcattaagcaatttctctagtgtgtcggacacaaataggttctttatttttggcatgtatgcctt tttattttcattcaallllULLtαLctcagacagacatagtagtatcaactagcattggaaaatacatatcactattcttggaatatttatggtcagtctactt tttagjaaaatatttttggatagcgttgacacgatagatcttattccatacttctttattattga tggagaagaggttgggcttttttgaaagagacaaaaatttattataacactaaacactccttttttgacatattaaagcctttattccatctctcaagata tattataaaatttatttttttaatttaagatttctgaattattttatctt^ tgatgaωaggaattttctctcttggaaaaggcttcccctgtgatgaaaatgatgtgccagctaaaattgtgtgccatttaaaaactgaaaatattttaa aattatttgtctatattctaaattgagctttggatcaaactttaggccaggaccagctcatgcgttctcattcttccttttctcactctttctctcatcactca cctctgtattcattctgttgtttgggatagaaaaatcataaagagccaacccatctcagaacgttgtggattgagagagacactacatgactccaag tatatgagaaaaggacagagctctaattgataactctgtag^tcaaaaggaaaagagtatgcccaattctctctacatgacatattgagattttttttaa tcaacttttaagatagtgatgflctgttctaaactgttctgttttagtgaaggtagatttttataaaacaagcatggggattcttttctaaggtaatattaat gagaagggaaaaaagtatctttaacagctctttgttgaagcctgtggtagcacattatgtttataattgcacatgtgcacataatctattatgatccaat gcaaatacagctccaaaaatattaaatgtatatatattttaaaatgcctgaggaaatacatttttcttaataaactgaagagtctcagtatggctattaaa ataattattagcctcctgttgtgtggctgcaaaacatcacaaagtgaccggtcttgagacctgtgaactgctgccctgtttagtaaataaaattaatgc atttctagagggggaatatctgccatccagtggtggaaatgtggagtaaagaagctggtggtctgcttctgtgctgtatgccagccttttgccttaa gttgagaggaggtcaactttagctactgtctttggtttgagagccatggcaaaaaaaaaaaaagaaaaaaagatcaagtcgtctttggtgagcca gtaaggtgaaagcttgctgactgtccaaggcacaagagaaaattgaggaattgaaatgcaacctgagtatcaaactaaatattctaatcaaaggt aggtactgttaggtggaattctatcagcaggcaactgcaaatgagaagaagatagaaggacgcccgtcgggactttggagggcattgttattttc ccaaagaaagacggccaagggcagaggcatggattctttgcagagcacttccttttggtttttcagtactgtttcatagacagtgggctcacatgtt cctgatagtgctgcagttgcttagaaagcatcccagttaattgcagtaattagaacttctggaatatgctagggcagaagtatgtcaagtatgtcac atgaagaaaatgtgaaattcaagagtaatccacacgtgagaaactagacaatgtacattcatgtgttctcttgaaaggaaagggagagctgtaag cttcactctgtcctacaccggagaaaagcaggaataactttaccgtggaaataatgtttagcttttatcagagaaaattgtccttctagagcatagag tcccaaaactcaattctggttttcccctgtαuutLtLlULlLULcccaacatatgaactgcagcatatcactttttctttttgtgcctcaggttcctcacctg taaaattgaaaaatatatgtattaataatattattaataataataatggtaatgtagtacttgtttgtaaagcactttgagatccttggttgaaaggcacca taggagtgccaagtattattatgtggccaagggggttatttaaactgtcagttcccaaaggccaggaaaggttggggtcatttttcttaaagacgag ctgtaaatatcaactaggcagccaatagtgttgactatgaagatgcaaaactattactaggctgataaaatcatagtttcttaatggctaccaataag gcaaatatcacaataataaacgccaaattccttagggcggactatttgacaaccacatggaaaactttgggggaggcatgaggggggaacatct caaaatgccaatgtaaaatttaacttacagcaatattcaccagcagaaaatgtctttcatatggaatgatttcatgttgctaagaaaaagaattcaattt gtagtcctgatttgaatactagaatgttggctataatagttctgttcttacaacacat ctactcacaaacatgttcttggtgtatttcttatgcaaacaatcttcaggcagcaaagatgtctgttacatctaaacttgaataataaagttttaccacca gttacaca
[0389] Following is a PRAME cDNA sequence (SEQ ID NO: 15). NM_006115 - PRAME gcttcagggtacagctcccccgcagccagaagccgggcctgcagcgcctcagcaccgctccgggacaccccacccgcttcccaggcgtga cctgtcaacagcaacttcgcggtgtggtgaactctctgaggaaaaaccattttgattattactctcagacgtgcgtggcaacaagtgactgagacc tagaaatccaagcgttggaggtcctgaggccagcctaagtcgcttcaaaatggaacgaaggcgtttgtggggttccattcagagccgatacatc agcatgagtgtgtggacaagcccacggagacttgtggagctggcagggcagagcctgctgaaggatgaggccctggccattgccgccctgg agttgctgcccagggagctcttcccgccactcttcatggcagcctttgacgggagacacagccagaccctgaaggcaatggtgcaggcctggc ccttcacctgcctccctctgggagtgctgatgaagggacaacatcttcacctggagaccttcaaagctgtgcttgatggacttgatgtgctccttgc ccaggaggttcgccccaggaggtggaaacttcaagtgctggatttacggaagaactctcatcaggacttctggactgtatggtctggaaacagg gccagtctgtactcatttccagagccagaagcagctcagcccatgacaaagaagcgaaaagtagatggtttgagcacagaggcagagcagcc cttcattccagtagaggtgctcgtagacctgttcctcaaggaaggtgcctgtgatgaattgttctcctacctcattgagaaagtgaagcgaaagaaa aatgtactacgcctgtgctgtaagaagctgaagatttttgcaatgcccatgcaggatatcaagatgatcctgaaaatggtgcagctggactctattg aagatttggaagtgacttgtacctggaagctacccaccttggcgaaattttctccttacctgggccagatgattaatctgcgtagactcctcctctcc cacatccatgcatcttcctacatttccccggagaaggaagagcagtatatcgcccagttcacctctcagttcctcagtctgcagtgcctgcaggct ctctatgtggactctttatttttccttagaggccgcctggatcagttgctcaggcacgtgatgaaccccttggaaaccctctcaataactaactgccg gctttcggaaggggatgtgatgcatctgtcccagagtcccagcgtcagtcagctaagtgtcctgagtctaagtggggtcatgctgaccgatgtaa gtcccgagcccctccaagctctgctggagagagcctctgccaccctccaggacctggtctttgatgagtgtgggatcacggatgatcagctcctt gccctcctgccttccctgagccactgctcccagcttacaaccttaagcttctacgggaattccatctccatatctgccttgcagagtctcctgcagca cctcatcgggctgagcaatctgacccacgtgctgtatcctgtccccctggagagttatgaggacatccatggtaccctccacctggagaggcttg cctatctgcatgccaggctcagggagttgctgtgtgagttggggcggcccagcatggtctggcttagtgccaacccctgtcctcactgtgggga cagaaccttctatgacccggagcccatcctgtgcccctgtttcatgcctaactagctgggtgcacatatcaaatgcttcattctgcatacttggacac taaagccaggatgtgcatgcatcttgaagcaacaaagcagccacagtttcagacaaatgttcagtgtgagtgaggaaaacatgttcagtgagga aaaaacattcagacaaatgttcagtgaggaaaaaaaggggaagttggggataggcagatgttgacttgaggagttaatgtgatctttggggagat acatcttatagagttagaaatagaatctgaatttctaaagggagattctggcttgggaagtacatgtaggagttaatccctgtgtagactgttgtaaa gaaactgttgaaaataaagagaagcaatgtgaagcaaaaaaaaaaaaaaaaaa
[0390] Following is a ZNF221 cDNA sequence (SEQ ID NO: 16). NM_013359 - ZNF221 gcgggagctacggctgcgggagttaccctttattctgtgatattttgagctgttctgcgtctcgcaggcccttctgaatttcctggacctactctcgg aaccctcaacgagctagcgatagatttagaggaaaggaagcaccgtcggaaagcaaagggatttctggaagcttgaacttaagagtgataactt agggtacctggcaaaagaaatttctgagcagcaaagcattcaagaagtggcctggctgcctcactgcttcaaacagcctacaattatatatggga gcaaaaaaatgacttaaagttggaatttatatttaaaaggcatgatttcaccttcacttgaactgcttcattcaggactctacaaattccctgaagtaga aggaaaaatgaccacattcaaagaggcagtgacattcaaggatgtggctgtggtcttcactgaggaggagctggggctgctggaccctgccca gaggaagctgtaccgagatgtgatgctagagaacttcaggaacctgctctcagtagggaatcaaccattccaccaagatactttccacttcttagg gaaggaaaagttttggaagatgaagacaacaagccaaagagaagggaattcaggaggcaagatccaaattgagatggagactgttccagaag caggaccacatgaagagtggtcctgtcagcaaatatgggaacaaattgcaagtgacctaaccaggtctcaaaactccataaggaacagctctca gttcttcaaagaaggtgatgtcccctgccagattgaggcaagactatctataagtcacgtgcaacagaaaccttaccgttgtaatgaatgtaaaca gtccatcagtgatgtttctgtctttgatcttcatcaacaatcacactcaggagagaaatctcatacatgtggtgagtgtggaaaaagcttctgttacag cccagcccttcatattcatcagagagtccatatgggagaaaaatgctataagtgtgatgtgtgtggtaaggaatttaatcagagctcacatctgcaa actcatcagagagtccatactggagagaaaccattcaaacgtgggcaatgtgggaaaggcttccatagtagatcagcacttaatgttcattgcaa attgcacacaggagagaaaccttataattgtgaggaatgtgggaaagccttcattcatgattcacagctccaggaacatcagagaatccatactg gggagaagccattcaaatgtgatatatgtggtaagagcttccgtgttagatcaagacttaataggcattccatggttcacacaggagaaaaaccat tcagatgtgatacatgtggcaagaactttcgtcagagatcagcacttaatagtcattccatggtccacatagaagagaagccatacaaatgtgagc aatgtggaaaaggcttcatttgtaggcgagatttttgtaagcatcagatggtccacacaggagagaaaccatataattgtaaagaatgtgggaaga ccttcagatggtcctcatgtcttttgaaccatcagcaagtccacagtggacaaaaatccttcaaatgtgaagaatgtgggaagggattttatacaaa ttcacgacgatcttcccatcagagatcccacaatggagaaaagccatataactgtgaggagtgtggtaaggactataaaaggaggttggatcttg agtttcaccagagggtccacacgggtgagagaccctataattgtaaggaatgtggcaagagctttggctgggcctcctgtcttttgaaacatcag agactccacagtggggaaaaacctttcaaatgtgaagagtgtggaaagagatttactcagagtacacaacttcattcccatcagacatgccatact ggagaaaagctatacaaatgtgagcagtgtgagaaggggtacaacagtaaatttaatcttgacatgcaccagagggtccacaggggagagcg accctataattgtaaggaatgtggaaagagctttggctgggcttcatgtcttttgaaacatcagagactccacagtggagaaaagccattgaaatct ggagtgtgggaagagatctactcagaattcacagcttcatttacatcagtaagtctatgtgggagaaaagccatataaatgtgagaagtgtggga agggctttggctgggcctcaactcatctgacccatcaattctccacagcagagaaaaaccattcaaatatgagaactgtgggaagagctttgtac atagatcatatctltLLtltltlLUlgagacagagtctcactctttcacccaagcctgactgcagtggcgctatctcagctcgctgcaagctccgcctcc caagttcacgccattctcctgcctcagcctcccgagtaactgggactacaggtgcccgccaccacacccagctaattttttgtatttttagtaaaaac ggggtttcaccgtgttagccaggatggtctcgatctcctga
[0391] Following is a first FOXA2 cDNA sequence (SEQ ID NO: 17). Exons and domains encoded by the nucleotide sequence are referenced, each of which is incorporated by reference herein. NM_021784 -FOXA2 cccgcccacttccaactaccgcctccggcctgcccagggagagagagggagtggagcccagggagagggagcgcgagagagggaggga ggaggggacggtgctttggctgacttttttttaaaagagggtgggggtggggggtgattgctggtcgtttgttgtggctgttaaa tgcactcggcttccagtatgctgggagcggtgaagatggaagggcacgagccgtccgactggagcagctactatgcagagcccgagggcta ctcctccgtgagcaacatgaacgccggcctggggatgaacggcatgaacacgtacatgagcatgtcggcggccgccatgggcagcggctcg ggcaacatgagcgcgggctccatgaacatgtcgtcgtacgtgggcgctggcatgagcccgtccctggcggggatgtcccccggcgcgggcg ccatggcgggcatgggcggctcggccggggcggccggcgtggcgggcatggggccgcacttgagtcccagcctgagcccgctcggggg gcaggcggccggggccatgggcggcctggccccctacgccaacatgaactccatgagccccatgtacgggcaggcgggcctgagccgcg cccgcgaccccaagacctacaggcgcagctacacgcacgcaaagccgccctactcgtacatctcgctcatcaccatggccatccagcagagc cccaacaagatgctgacgctgagcgagatctaccagtggatcatggacctcttccccttctaccggcagaaccagcagcgctggcagaactcc atccgccactcgctctccttcaacgactgtttcctgaaggtgccccgctcgcccgacaagcccggcaagggctccttctggaccctgcaccctg actcgggcaacatgttcgagaacggctgctacctgcgccgccagaagcgcttcaagtgcgagaagcagctggcgctgaaggaggccgcag gcgccgccggcagcggcaagaaggcggccgccggagcccaggcctcacaggctcaactcggggaggccgccgggccggcctccgaga ctccggcgggcaccgagtcgcctcactcgagcgcctccccgtgccaggagcacaagcgagggggcctgggagagctgaaggggacgcc ggctgcggcgctgagccccccagagccggcgccctctcccgggcagcagcagcaggccgcggcccacctgctgggcccgccccaccac ccgggcctgccgcctgaggcccacctgaagccggaacaccactacgccttcaaccacccgttctccatcaacaacctcatgtcctcggagcag cagcaccaccacagccaccaccaccaccaaccccacaaaatggacctcaaggcctacgaacaggtgatgcactaccccggctacggttccc ccatgcctggcagcttggccatgggcccggtcacgaacaaaacgggcctggacgcctcgcccctggccgcagatacctcctactaccaggg ggtgtactcccggcccattatgaactcctottaagaagacgacggcttcaggcccggctaactctggcaccccggatcgagga[c/t]aagtga gagagcaagtgggggtcgagactttggggagacggtgttgcagagacgcaagggagaagaaatccataacacccccaccccaacaccccc aagacagcagtcttcttcacccgctgcagccgttccgtcccaaacagagggccacacagataccccacgttctatataaggaggaaaacggga aagaatataaagttaaaaaaaagcctccggtttccactactgtgtagactcctgcttcttcaagcacctgcagattctgatttttttgttgttgttgttctc ctccattgctgttgttgcagggaagtcttacttaaaaaaaaaaaaaaattttgtgagtgactcggtgtaaaaccatgtagttttaacagaaccagagg gttgtactattgtttaaaaacaggaaaaaaaataatgtaagggtctgttgtaaatgaccaagaaaaagaaaaaaaaagcattcccaatcttgacac ggtgaaatccaggtctcgggtccgattaatttatggtttctgcgtgctttatttatggcttataaatgtgtattctggctgcaagggccagagttccaca aatctatattaaagtgttatacccggtttt
Exon and Domain Characterization
Figure imgf000606_0001
[0392} Following is a second FOXA2 cDNA sequence (SEQ ID NO: 18). Exons and domains encoded by the nucleotide sequence are referenced, each of which is incorporated by reference herein.
NM_153675 - FOXA2 tcggccgctgctagaggggctgcttgcgccaggcgccggccgccccactgcgggtccctggcggccggtgtctgaggagtcggagagccg aggcggccagaccgtgcgccccgcgcttctcccgaggccgttccgggtctgaactgtaacagggaggggcctcgcaggagcagcagcggg cgagttaaagtatgctgggagcggtgaagatggaagggcacgagccgtccgactggagcagctactatgcagagcccgagggctactcctc cgtgagcaacatgaacgccggcctggggatgaacggcatgaacacgtacatgagcatgtcggcggccgccatgggcagcggctcgggcaa catgagcgcgggctccatgaacatgtcgtcgtacgtgggcgctggcatgagcccgtccctggcggggatgtcccccggcgcgggcgccatg gcgggcatgggcggctcggccggggcggccggcgtggcgggcatggggccgcacttgagtcccagcctgagcccgctcggggggcagg cggccggggccatgggcggcctggccccctacgccaacatgaactccatgagccccatgtacgggcaggcgggcctgagccgcgcccgc gaccccaagacctacaggcgcagctacacgcacgcaaagccgccctactcgtacatctcgctcatcaccatggccatccagcagagccccaa caagatgctgacgctgagcgagatctaccagtggatcatggacctcttccccttctaccggcagaaccagcagcgctggcagaactccatccg ccactcgctctccttcaacgactgtttcctgaaggtgccccgctcgcccgacaagcccggcaagggctccttctggaccctgcaccctgactcg ggcaacatgttcgagaacggctgctacctgcgccgccagaagcgcttcaagtgcgagaagcagctggcgctgaaggaggccgcaggcgcc gccggcagcggcaagaaggcggccgccggagcccaggcctcacaggctcaactcggggaggccgccgggccggcctccgagactccg gcgggcaccgagtcgcctcactcgagcgcctccccgtgccaggagcacaagcgagggggcctgggagagctgaaggggacgccggctg cggcgctgagccccccagagccggcgccctctcccgggcagcagcagcaggccgcggcccacctgctgggcccgccccaccacccggg cctgccgcctgaggcccacctgaagccggaacaccactacgccttcaaccacccgttctccatcaacaacctcatgtcctcggagcagcagca ccaccacagccaccaccaccaccaaccccacaaaatggacctcaaggcctacgaacaggtgatgcactaccccggctacggttcccccatgc ctggcagcttggccatgggcccggtcacgaacaaaacgggcctggacgcctcgcccctggccgcagatacctcctactaccagggggtgtac tcccggcccattatgaactcctcttaagaagacgacggcttcaggcccggctaactctggcaccccggatcgagga[c/t]aagtgagagagca agtgggggtcgagactttggggagacggtgttgcagagacgcaagggagaagaaatccataacacccccaccccaacacccccaagacag cagtcttcttcacccgctgcagccgttccgtcccaaacagagggccacacagataccccacgttctatataaggaggaaaacgggaaagaatat aaagttaaaaaaaagcctccggtttccactactgtgtagactcctgcttcttcaagcacctgcagattctgatttttttgttgttgttgttctc ctgttgttgcagggaagtcttacttaaaaaaaaaaaaaaattttgtgagtgactcggtgtaaaaccatgtagttttaacagaaccagagggttgtact attgtttaaaaacaggaaaaaaaataatgtaagggtctgttgtaaatgaccaagaaaaagaaaaaaaaagcattcccaatcttgacacggtgaaat ccaggtctcgggtccgattaatttatggtttctgcgtgctttatttatggcttataaatgtgtattctggctgcaagggccagagttccacaaatctatat taaagtgttatacccggtttt
Exon and Domain Characterization
Figure imgf000607_0001
[0393] Following is a JAMS cDNA sequence (SEQ ID NO: 19). NM_032801 - Homo sapiens junctional adhesion molecule 3 (JAM3), mRNA.
TAGACCTCAGCTTCCTCTGTCACCATGGTGCCGGCTCGGCTGGGCCCGGCGGTCGCCAT
GGTAACTGGGGCGGGTCGCAGGGTCCTGGCAGGCTGGGCGCATGCGCGCGGGGACTA
CAAGCCGCGCCGCGCTGCCGCTGGCCCCTCAGCAACCCTCGACATGGCGCTGAGGCGG
CCACCGCGACTCCGGCTCTGCGCTCGGCTGCCTGACTTCTTCCTGCTGCTGCTTTTCAGG
GGCTGCCTGATAGGGGCTGTAAATCTCAAATCCAGCAATCGAACCCCAGTGGTACAGG
AATTTGAAAGTGTGGAACTGTCTTGCATCATTACGGATTCGCAGACAAGTGACCCCAG
GATCGAGTGGAAGAAAATTCAAGATGAACAAACCACATATGTGTTTTTTGACAACAAA
ATTCAGGGAGACTTGGCGGGTCGTGCAGAAATACTGGGGAAGACATCCCTGAAGATCT
GGAATGTGACACGGAGAGACTCAGCCCTTTATCGCTGTGAGGTCGTTGCTCGAAATGA
CCGCAAGGAAATTGATGAGATTGTGATCGAGTTAACTGTGCAAGTGAAGCCAGTGACC
CCTGTCTGTAGAGTGCCGAAGGCTGTACCAGTAGGCAAGATGGCAACACTGCACTGCC
AGGAGAGTGAGGGCCACCCCCGGCCTCACTACAGCTGGTATCGCAATGATGTACCACT
GCCCACGGATTCCAGAGCCAATCCCAGATTTCGCAATTCTTCTTTCCACTTAAACTCTG
AAACAGGCACTTTGGTGTTCACTGCTGTTCACAAGGACGACTCTGGGCAGTACTACTGC
ATTGCTTCCAATGACGCAGGCTCAGCCAGGTGTGAGGAGCAGGAGATGGAAGTCTATG
ACCTGAACATTGGCGGAATTATTGGGGGGGTTCTGGTTGTCCTTGCTGTACTGGCCCTG
ATCACGTTGGGCATCTGCTGTGCATACAGACGTGGCTACTTCATCAACAATAAACAGG
ATGGAGAAAGTTACAAGAACCCAGGGAAACCAGATGGAGTTAACTACATCCGCACTG
ACGAGGAGGGCGACTTCAGACACAAGTCATCGTTTGTGATCTGAGACCCGCGGTGTGG
CTGAGAGCGCACAGAGCGCACGTGCACATACCTCTGCTAGAAACTCCTGTCAAGGCAG
CGAGAGCTGATGCACTCGGACAGAGCTAGACACTCATTCAGAAGCTTTTCGTTTTGGCC
AAAGTTGACCACTACTCTTCTTACTCTAACAAGCCACATGAATAGAAGAATTTTCCTCA
AGATGGACCCGGTAAATATAACCACAAGGAAGCGAAACTGGGTGCGTTCACTGAGTTG
GGTTCCTAATCTGTTTCTGGCCTGATTCCCGCATGAGTATTAGGGTGATCTTAAAGAGT
TTGCTCACGTAAACGCCCGTGCTGGGCCCTGTGAAGCCAGCATGTTCACCACTGGTCGT
TCAGCAGCCACGACAGCACCATGTGAGATGGCGAGGTGGCTGGACAGCACCAGCAGC
GCATCCCGGCGGGAACCCAGAAAAGGCTTCTTACACAGCAGCCTTACTTCATCGGCCC
ACAGACACCACCGCAGTTTCTTCTTAAAGGCTCTGCTGATCGGTGTTGCAGTGTCCATT
GTGGAGAAGCTTTTTGGATCAGCATTTTGTAAAAACAACCAAAATCAGGAAGGTAAAT
TGGTTGCTGGAAGAGGGATCTTGCCTGAGGAACCCTGCTTGTCCAACAGGGTGTCAGG
ATTTAAGGAAAACCTTCGTCTTAGGCTAAGTCTGAAATGGTACTGAAATATGCTTTTCT
ATGGGTCTTGTTTATTTTATAAAATTTTACATCTAAATTTTTGCTAAGGATGTATTTTGA
TTATTGAAAAGAAAATTTCTATTTAAACTGTAAATATATTGTCATACAATGTTAAATAA
CCTATTTTTTTAAAAAAGTTCAACTTAAGGTAGAAGTTCCAAGCTACTAGTGTTAAATT
GGAAAATATCAATAATTAAGAGTATTTTACCCAAGGAATCCTCTCATGGAAGTTTACTG
TGATGTTCCTTTTCTCACACAAGTTTTAGCCTTTTTCACAAGGGAACTCATACTGTCTAC
ACATCAGACCATAGTTGCTTAGGAAACCTTTAAAAATTCCAGTTAAGCAATGTTGAAA
TCAGTTTGCATCTCTTCAAAAGAAACCTCTCAGGTTAGCTTTGAACTGCCTCTTCCTGA
GATGACTAGGACAGTCTGTACCCAGAGGCCACCCAGAAGCCCTCAGATGTACATACAC AGATGCCAGTCAGCTCCTGGGGTTGCGCCAGGCGCCCCCGCTCTAGCTCACTGTTGCCT
CGCTGTCTGCCAGGAGGCCCTGCCATCCTTGGGCCCTGGCAGTGGCTGTGTCCCAGTGA
GCTTTACTCACGTGGCCCTTGCTTCATCCAGCACAGCTCTCAGGTGGGCACTGCAGGGA
CACTGGTGTCTTCCATGTAGCGTCCCAGCTTTGGGCTCCTGTAACAGACCTCTTTTTGGT
TATGGATGGCTCACAAAATAGGGCCCCCAATGCTATTTTTTTTTTTTAAGTTTGTTTAAT
TATTTGTTAAGATTGTCTAAGGCCAAAGGCAATTGCGAAATCAAGTCTGTCAAGTACA
ATAACATTTTTAAAAGAAAATGGATCCCACTGTTCCTCTTTGCCACAGAGAAAGCACCC
AGACGCCACAGGCTCTGTCGCATTTCAAAACAAACCATGATGGAGTGGCGGCCAGTCC
AGCCTTTTAAAGAACGTCAGGTGGAGCAGCCAGGTGAAAGGCCTGGCGGGGAGGAAA
GTGAAACGCCTGAATCAAAAGCAGTTTTCTAATTTTGACTTTAAATTTTTCATCCGCCG
GAGACACTGCTCCCATTTGTGGGGGGACATTAGCAACATCACTCAGAAGCCTGTGTTCT
TCAAGAGCAGGTGTTCTCAGCCTCACATGCCCTGCCGTGCTGGACTCAGGACTGAAGT
GCTGTAAAGCAAGGAGCTGCTGAGAAGGAGCACTCCACTGTGTGCCTGGAGAATGGCT
CTCACTACTCACCTTGTCTTTCAGCTTCCAGTGTCTTGGGTTTTTTATACTTTGACAGCT
TTTTTTTAATTGCATACATGAGACTGTGTTGACTTTTTTTAGTTATGTGAAACACTTTGC
CGCAGGCCGCCTGGCAGAGGCAGGAAATGCTCCAGCAGTGGCTCAGTGCTCCCTGGTG
TCTGCTGCATGGCATCCTGGATGCTTAGCATGCAAGTTCCCTCCATCATTGCCACCTTG
GTAGAGAGGGATGGCTCCCCACCCTCAGCGTTGGGGATTCACGCTCCAGCCTCCTTCTT
GGTTGTCATAGTGATAGGGTAGCCTTATTGCCCCCTCTTCTTATACCCTAAAACCTTCTA
CACTAGTGCCATGGGAACCAGGTCTGAAAAAGTAGAGAGAAGTGAAAGTAGAGTCTG
GGAAGTAGCTGCCTATAACTGAGACTAGACGGAAAAGGAATACTCGTGTATTTTAAGA
TATGAATGTGACTCAAGACTCGAGGCCGATACGAGGCTGTGATTCTGCCTTTGGATGG
ATGTTGCTGTACACAGATGCTACAGACTTGTACTAACACACCGTAATTTGGCATTTGTT
TAACCTCATTTATAAAAGCTTCAAAAAAACCCAAAAAAACCCAAA
[0394] Following is a GLB cDNA sequence (SEQ ID NO: 20).
NM_000168 - Homo sapiens GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) (GLI3), mRNA.
CGATACTACGTGGGCATTTTTGGTCGAAGAGAGCTGAAGTAATGAGAAGACATCATGG
AGGCCCAGTCCCACAGCTCCACGACCACTGAAAAGAAAAAAGTTGAGAATTCCATAGT
GAAGTGCTCCACTCGAACAGATGTGAGCGAGAAAGCCGTTGCCTCCAGCACCACTTCT
AATGAGGATGAAAGTCCTGGACAGACTTATCACAGAGAGAGAAGAAACGCAATCACT
ATGCAGCCACAGAATGTCCAGGGGCTCAGCAAAGTCAGTGAGGAACCTTCAACATCGA
GTGACGAGAGGGCCTCATTGATCAAGAAAGAGATCCATGGGTCCCTGCCACACGTGGC
GGAGCCCTCTGTGCCGTACCGCGGGACGGTGTTTGCCATGGACCCCAGGAATGGTTAC
ATGGAGCCCCACTACCACCCTCCTCATCTTTTCCCTGCCTTCCATCCTCCTGTACCAATT
GATGCCAGACATCATGAGGGCCGTTACCATTACGATCCATCTCCGATTCCTCCATTGCA
TATGACTTCCGCCTTATCTAGTAGCCCTACGTATCCGGACCTGCCCTTCATTAGGATCTC
CCCACACCGGAACCCCGCTGCTGCTTCCGAGTCTCCCTTCAGCCCTCCACATCCCTACA
TTAATCCCTACATGGACTATATCCGCTCCTTGCACAGCAGCCCATCGCTCTCCATGATC
TCAGCAACCCGTGGGCTGAGCCCTACAGATGCGCCCCATGCAGGAGTCAGCCCAGCAG
AATACTATCATCAGATGGCCCTGCTAACTGGCCAGCGCAGCCCCTATGCAGACATTATT
CCCTCAGCTGCCACCGCCGGCACGGGGGCCATCCACATGGAATATCTTCATGCTATGG
ATAGCACCAGATTCTCCAGCCCCAGGCTGTCAGCCAGGCCGAGCCGAAAACGTACACT
GTCCATATCACCACTCTCCGATCATAGCTTTGACCTTCAGACCATGATAAGGACGTCTC
CCAACTCCTTGGTCACGATTCTCAATAATTCCCGTAGCAGCTCTTCAGCAAGTGGCTCC
TATGGTCACTTATCTGCAAGTGCAATCAGCCCTGCCTTGAGCTTCACCTACTCTTCCGC
GCCCGTCTCTCTCCACATGCATCAGCAGATCCTAAGCCGACAACAGAGCTTAGGTTCA
GCCTTTGGACACAGCCCTCCACTCATCCACCCTGCCCCAACTTTTCCAACACAGAGGCC
TATTCCAGGGATCCCTACGGTTCTGAACCCCGTCCAGGTCAGCTCCGGCCCTTCTGAGT
CCTCACAGAACAAGCCCACGAGTGAGTCTGCAGTGAGCAGCACTGGTGACCCGATGCA
CAACAAGAGGTCCAAGATCAAACCCGATGAAGACCTCCCCAGCCCAGGGGCTCGGGG GCAGCAGGAACAGCCCGAAGGAACAACCCTTGTCAAGGAGGAAGGGGACAAAGATGA
AAGCAAACAGGAGCCTGAAGTCATCTATGAGACAAACTGCCACTGGGAAGGCTGCGC
GAGGGAGTTCGACACCCAAGAGCAGCTTGTGCACCATATAAATAACGACCATATTCAT
GGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTGGACTGCTCAAGAGAGCAGAAACCC
TTCAAAGCCCAGTATATGTTGGTAGTGCATATGAGAAGACACACGGGCGAGAAGCCTC
ACAAATGCACTTTTGAAGGTTGCACAAAGGCCTACTCGAGACTAGAAAACTTGAAAAC
ACACTTGAGATCTCACACTGGAGAGAAACCATACGTCTGTGAGCACGAAGGTTGCAAC
AAGGCTTTCTCAAATGCCTCTGATCGCGCCAAACACCAAAACAGAACGCATTCCAATG
AGAAACCATATGTGTGCAAAATCCCAGGCTGCACTAAGCGTTACACAGACCCAAGCTC
CCTCCGGAAACATGTGAAGACAGTGCATGGCCCAGAGGCTCATGTCACCAAGAAGCAG
CGAGGGGACATCCATCCTCGGCCGCCACCCCCGAGAGATTCCGGCAGCCATTCACAGT
CCAGGTCGCCTGGCCGACCGACTCAGGGAGCCCTTGGTGAGCAGCAGGACCTCAGCAA
CACTACCTCAAAGCGGGAAGAATGCCTCCAGGTGAAAACCGTCAAGGCAGAGAAGCC
AATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCC
ATCAGCAACTATTCCAACAGTGGGCTCGAGCTTCCTCTGACCGATGGAGGTAGTATAG
GAGACCTCAGTGCCATCGATGAAACCCCAATCATGGACTCAACCATTTCCACTGCAAC
CACAGCCCTTGCTTTGCAAGCCAGGAGAAACCCGGCAGGGACCAAATGGATGGAGCA
CGTAAAACTAGAAAGGCTAAAACAAGTGAATGGAATGTTTCCGCGACTGAACCCCATT
CTACCCCCTAAAGCCCCTGCGGTCTCTCCTCTCATAGGAAATGGCACACAGTCCAACAA
CACCTGCAGCTTGGGTGGGCCCATGACGCTTCTCCCGGGCAGAAGCGACCTCTCTGGG
GTGGACGTCACTATGCTGAACATGCTCAACAGAAGGGACAGCAGCGCCAGCACCATCA
GCTCGGCCTACCTGAGCAGCCGCCGCTCCTCAGGGATCTCGCCCTGCTTCTCCAGCCGC
CGCTCCAGCGAGGCGTCACAGGCCGAGGGCCGGCCGCAGAACGTGAGCGTGGCCGAC
TCCTACGACCCCATCTCCACCGACGCCTCGCGCCGCTCCAGCGAAGCCAGCCAGAGCG
ACGGCCTGCCCAGCCTGCTCAGCCTCACGCCCGCCCAGCAGTACCGCCTCAAGGCCAA
GTACGCGGCTGCCACAGGAGGGCCGCCGCCGACGCCCCTGCCCAACATGGAGAGGAT
GAGCCTGAAGACGCGCCTGGCGCTGCTCGGGGATGCCCTCGAGCCTGGCGTGGCCCTG
CCTCCAGTTCATGCCCCGAGGAGGTGCAGCGACGGGGGAGCCCACGGCTACGGGCGGC
GCCACCTGCAGCCGCACGATGCGCTGGGCCACGGCGTGAGGAGGGCCAGCGACCCGG
TGCGGACAGGCTCCGAGGGCCTGGCCCTGCCTCGTGTGCCGCGCTTCAGCAGCCTCAG
CAGCTGCAACCCCCCGGCGATGGCCACGTCCGCGGAGAAGCGCAGTCTCGTGCTTCAG
AATTACACGCGGCCCGAGGGCGGCCAGTCCCGAAACTTCCACTCGTCCCCCTGTCCTCC
CAGCATCACCGAGAACGTCACCCTGGAGTCCCTGACCATGGACGCTGATGCCAACCTG
AACGATGAGGATTTCCTGCCGGACGACGTGGTGCAGTATTTAAATTCCCAGAACCAAG
CAGGGTACGAGCAGCACTTCCCCAGCGCCCTCCCGGACGACAGCAAAGTGCCCCACGG
GCCCGGTGACTTTGACGCGCCCGGGCTGCCAGACAGCCACGCTGGCCAGCAGTTCCAT
GCCCTCGAGCAGCCCTGCCCCGAGGGCAGCAAAACCGACCTGCCCATTCAGTGGAACG
AAGTCAGCTCCGGAAGCGCCGACCTGTCCTCCTCCAAGCTCAAGTGTGGGCCGCGGCC
CGCTGTGCCGCAGACTCGCGCCTTTGGGTTCTGCAACGGCATGGTCGTCCACCCGCAGA
ACCCCTTGAGGAGCGGGCCTGCTGGGGGCTATCAGACCCTCGGGGAGAACAGCAACCC
CTACGGTGGCCCAGAGCACTTGATGCTCCACAACAGCCCCGGAAGTGGCACCAGTGGA
AACGCCTTCCATGAACAGCCCTGTAAGGCCCCGCAGTATGGGAACTGTCTCAACAGGC
AGCCAGTGGCCCCTGGTGCACTCGACGGTGCCTGTGGTGCCGGGATTCAAGCCTCAAA
GCTGAAGAGCACCCCCATGCAAGGGAGCGGGGGCCAGCTGAATTTCGGCCTGCCGGTA
GCGCCAAATGAGTCAGCTGGCAGCATGGTGAATGGCATGCAGAACCAGGACCCAGTG
GGACAGGGGTACCTGGCTCACCAGCTCCTCGGCGACAGCATGCAGCACCCGGGGGCAG
GCCGCCCCGGTCAGCAGATGCTTGGGCAGATTAGTGCTACCTCACACATCAACATCTA
CCAAGGGCCAGAGAGCTGCCTGCCAGGGGCTCACGGCATGGGCAGCCAGCCGTCAAG
CTTGGCAGTTGTCAGGGGCTACCAGCCATGTGCCAGCTTTGGGGGCAGCAGGCGCCAG
GCTATGCCGAGGGACAGCCTTGCTCTGCAGTCAGGACAGCTCAGTGACACAAGTCAGA
CCTGCAGGGTGAATGGTATCAAGATGGAGATGAAAGGGCAGCCCCATCCGCTGTGCTC
TAATCTGCAGAATTACTCTGGTCAGTTCTATGACCAAACCGTGGGCTTCAGTCAGCAAG
ACACGAAAGCTGGTTCATTCTCTATTTCAGACGCCAGCTGCCTGCTACAGGGGACCAG CGCCAAAAACTCTGAGTTACTTTCCCCAGGTGCTAATCAGGTGACAAGCACAGTGGAC
AGCCTCGACAGCCATGACCTGGAAGGGGTACAGATTGACTTCGATGCCΆTCATAGACG
ATGGGGACCACTCCAGCCTGATGTCGGGGGCCCTGAGCCCAAGTATCATTCAGAACCT
TTCCCATAGCTCCTCCCGCCTCACCACGCCTCGGGCGTCCCTCCCATTCCCAGCGCTGT
CCATGAGCACCACCAACATGGCTATCGGGGACATGAGTTCTTTGCTGACCTCCCTAGCG
GAAGAAAGCAAATTCCTTGCAGTTATGCAATAGGCTTTAGGAAAAAAAGACTGCAACC
AACGGAAATCAATAGGAGTTGAAGAGATTAAACTGACTTTGTTTTGGCTGTTTTTTTAG
TTCTGTATGTATTTTAGCAATCTCATCTCACCTAACTGAGATGTGTTTCAATTATATTCC
TTTTATGGAAAAGGACTCTGAAAAACCCTAAAGTATTCTAGGGAGAAACTGTCTTCCA
TTTCAGTTTTGAATCAGTATTGTTACACTCAAACCACCCTCTTTTTAAAAAAAAA
[0395] Following is a first SLC22A1 amino acid sequence (SEQ ID NO: 21). NP_003048 - SLC22A1 isoform A
MPTVDDH.EQVGESGWFQKQAFLILCLLSAAFAPICVGIVFLGFTPDHHCQSPGVAELSQRC
GWSP AEELNYTVPGLGP AGEAFLGQCRRYEVD WNQSALSCVDPLASLATNRSHLPLGPCQ
DGWVYDTPGSSΓVTEFNLVCADSWKLDLFQSCLNAGFLFGSLGVGYF ADRFGRKLCLLGT
VLVNAVSGVLMAFSPNYMSMLLFRLLQGLVSKGNWMAGYTLITEFVGSGSRRTVAIMYQ
MAFTVGLVALTGLAYALPHWRWLQLAVSLPTFLFLLYYWCVPESPRWLLSQKRNTEAIKI
MDHIAQKNGKLPPADLKMLSLEEDVTEKLSPSFADLFRTPRLRKRTFILMYLWFTDSVLYQ
GLILHMGATSGNLYLDFLYSALVEIPGAFIALITIDRVGRIYPMAMSNLLAGAACLVMIFISP
DLHWLNIIIMCVGRMGITIAIQMICLVNAELYPTFVRNLGVMVCSSLCDIGGIITPFIVFRLRE
VWQALPLILFAVLGLLAAGVTLLLPETKGVALPETMKDAENLGRKAKPKENTIYLKVQTS
EPSGT
[0396] Following is a second SLC22A1 amino acid sequence (SEQID NO: 22). NP_694857
MPTVDDILEQVGESGWFQKQAFLILCLLSAAF APICVGIVFLGFTPDHHCQSPGV AE
LSQRCGWSP AEELNYTVPGLGP AGEAFLGQCRRYEVD WNQSALSCVDPLASLATN
RSHLPLGPCQDGWVYDTPGSSIVTEFNLVCADSWKLDLFQSCLNAGFLFGSLGVG
YFADRFGRKLCLLGTVLVNAVSGVLMAFSPNYMSMLLFRLLQGLVSKGNWMAG
YTLITEFVGSGSRRTVAIMYQMAFTVGLVALTGLAYALPHWRWLQLAVSLPTFLF
LLYYWCVPESPRWLLSQKRNTEAIKIMDHIAQKNGKLPPADLKMLSLEEDVTEKLS
PSFADLFRTPRLRKRTFILMYLWFTDSVLYQGLILHMGATSGNLYLDFLYSALVEIP
GAFIALITIDRVGRI YPMAMSNLLAGAACLVMIFISPDLHWLNIIIMCVGRMGITIAI
QMICLVNAELYPTFVSGVGPACRGSDATSSRX)QGGRFARDHEGRREPWEKSKAQR
KHDLP
[0397] Following is a TTN amino acid sequence (SEQ ID NO: 23). Other isoform amino acid sequences are described and each is incorporated herein by reference in its entirety.
MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVPEVSWFRDGQVISTSTLPGVQISFSDGRA KLTIPAVTKANSGRYSLKATNGSGQATSTAELLVKAETAPPNFVQRLQSMTVRQGSQVRL QVRVTGIPTPVVKFYRDGAEIQSSLDFQISQEGDLYSLLIAEAYPEDSGTYSVNATNSVGRA TSTAELLVQGEEEVPAKKTKTIVSTAQISESRQTRIEKKIEAHFDARSIATVEMVIDGAAGQQ LPHKTPPRIPPKPKSRSPTPPSIAAKAQLARQQSPSPIRHSPSPVRHVRAPTPSPVRSVSPAARI STSPIRSVRSPLLMRKTQASTVATGPEVPPPWKQEGYVASSSEAEMRETTLTTSTQIRTEER WEGRYGVQEQVTISGAAGAAASVSASASYAAEAVATGAKEVKQDADKSAAVATWAAV DMARVREPVISAVEQTAQRTTTTAVHIQPAQEQVRKEAEKTAVTKWVAADKAKEQELK SRTKEVITTKQEQMHVTHEQIRKETEKTFVPKVVISAAKAKEQETRISEEITKKQKQVTQEA IRQETEITAASMVWATAKSTKLETVPGAQEETTTQQDQMHLSYEKIMKETRKTWPKVrV ATPKVKEQDLVSRGREGITTKREQVQITQEKMRKEAEKTALSTIAVATAKAKEQETILRTR
ETMATRQEQIQVTHGKVDVGKKAEAVATVVAAVDQARVREPREPGHLEESYAQQTTLEY
GYKERISAAKVAEPPQRPASEPHVVPKAVKPRVIQAPSETHIKTTDQKGMHISSQIKKTTDL
TTERLVHVDKRPRTASPHFTVSKISVPKTEHGYEASIAGSAIATLQKELSATSSAQKITKSVK
APTVKPSETRVRAEPTPLPQFPFADTPDTYKSEAGVEVKKEVGVSITGTTVREERFEVLHGR
EAKVTETARVP APVEIPVTPPTLVSGLKNVTVIEGESVTLECHISGYPSPTVTWYREDYQIES
SIDFQITFQSGIARLMIREAFAEDSGRFTCSAVNEAGTVSTSCYLAVQVSEEFEKETTAVTEK
FTTEEKRFVESRD WMTDTSLTEEQAGPGEP AAPYFITKPWQKLVEGGSWFGCQVGGNP
KPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYTIVVRNKHGETSAS
ASLLEEADYELLMKSQQEMLYQTQVTAFVQEPKVGETAPGFVYSEYEKEYEKEQALIRKK
MAKDTVVVRTYVEDQEFHISSFEERLIKEIEYRIIKTTLEELLEEDGEEKMAVDISESEAVES
GFDLRIKNΎRILEGMGVTFHCKMSGYPLPKIAWYKDGKRIKHGERYQMDFLQDGRASLRI
PVVLPEDEGIYTAFASNIKGNAICSGKLYVEPAAPLGAPTYIPTLEPVSRIRSLSPRSVSRSPIR
MSPARMSPARMSPARMSPARMSPGRRLEETDESQLERLYKPVFVLKPVSFKCLEGQTARF
DLKVVGRPMPETFWFHDGQQIVNDYTHKVVIKEDGTQSLIΓVPATPSDSGEWTWAQNRA
GRSSISVILTVEAVEHQVKPMFVEKXKNVNIKEGSRLEMKVRATGNPNPDΓVWLKNSDIΓVP
HKYPKIRIEGTKGEAALKIDSTVSQDSAWYTATAINKAGRDTTRCKVNVEVEFAEPEPERK
LIIPRGTYRAKEIAAPELEPLHLRYGQEQWEEGDLYDKEKQQKPFFKKKLTSLRLKRFGPA
HFECRLTPIGDPTMVVEWLHDGKPLEAANRLRMINEFGYCSLDYGVAYSRDSGΠTCRATN
KYGTDHTSATLΓVKDEKSLVEESQLPEGRKGLQRIEELERMAHEGALTGVTTDQKEKQKPD
IVLYPEPVRVLEGETARFRCRVTGYPQPKVNWYLNGQLIRKSKRFRVRYDGIHYLDIVDCK
SYDTGEVKVTAENPEGVIEHKVKLEIQQREDFRSVLRRAPEPRPEFHVHEPGKLQFEVQKV
DRPVDTTETKEWKLKRAERITHEKVPEESEELRSKFKRRTEEGYYEAITAVELKSRKKDES
YEELLRKTKDELLHWTKELTEEEKKALAEEGKITIPTFKPDKIELSPSMEAPKIFERIQSQTV
GQGSDAHFRVRVVGKPDPECEWYKNGVKIERSDRIYWYWPEDNVCELVIRDVTAEDSASI
MVKAINIAGETSSHAFLLVQAKQLITFTQELQDWAKEKDTMATFECETSEPFVKVKWYK
DGMEVHEGDKYRMHSDRKVHFLSILTIDTSDAEDYSCVLVEDENVKTTAKLΓVEGAVVEF
VKELQDIEVPESYSGELECIVSPENIEGKWYHNDVELKSNGKYTITSRRGRQNLTVKDVTK
EDQGEYSFVIDGKKTTCKLKMKPRPIAILQGLSDQKVCEGDIVQLEVKVSLESVEGVWMK
DGQEVQPSDRVHIVIDKQSHMLLIEDMTKEDAGNYSFTIPALGLSTSGRVSVYSVDVITPLK
DVNVIEGTKAVLECKVSVPDVTSVKWLNDEQIKPDDRVQAIVKGTKQRLVINRTHASDE
GPYKLIVGRVETNCNLSVEKIKIIRGLRDLTCTETQNVVFEVELSHSGIDVLWNFKDKEIKPS
SKYKIEAHGKIYKLTVLNMMKDDEGKYTFYAGENITSGKLTVAGGAISKPLTDQTVAESQ
EAVFECEVANPDSKGEWLRDGKHLPLTNNIRSESDGHKRRLIIAATKLDDIGEYTYKVATS
KTSAKLKVEAVKIKKTLKNLTVTETQDAVFTVELTHPNVKGVQWIKNGVVLESNEKYAIS
VKGTIYSLRIKNCAIVDESVYGFRLGRLGASARLHVETVKΠKKPKDVTALENATV AFEVSV
SHDTVPVKWFHKSVEIKPSDKHRLVSERKVHKLMLQNISPSDAGEYTAVVGQLECKAKLF
VETLHITKTMKNIEVPETKTASFECEVSHFNVPSMWLKNGVEIEMSEKFKΓWQGKLHQLΠ
MNTSTEDSAEYTFVCGNDQVSATLTVTPIMITSMLKDINAEEKDTITFEVTVNYEGISYKWL
KNGVEIKSTDKCQMRTKKLTHSLNIRNVHFGDAADYTFVAGKATSTATLYVEARHIEFRK
HIKDIKVLEKKRAMFECEVSEPDITVQWMKDDQELQITDRIKIQKEKYVHRLLIPSTRMSDA
GKYTWAGGNVSTAKLFVEGRDVRIRSIKKEVQVIEKQRAVVEFEVNEDDVDAHWYKDGI
EINFQVQERHKYVVERRIHRMFISETRQSDAGEYTFVAGRNRSSVTLYVNAPEPPQVLQEL
QPVTVQSGKPARFCAVISGRPQPKISWYKEEQLLSTGFKCKFLHDGQEYTLLLIEAFPEDAA
VYTCEAKNDYGVATTSASLSVEVPEWSPDQEMPVYPPAIITPLQDTVTSEGQPARFQCRV
SGTDLKVSWYSKDKKIKPSRFFRMTQFEDTYQLEIAEAYPEDEGTYTFVASNAVGQVSSTA
NLSLEAPESILHERIEQEIEMEMKEFSSSFLSAEEEGLHSAELQLSKINETLELLSESPVYSTKF
DSEKEGTGPIFIKEVSNADISMGDVATLSVTVIGIPKPKIQWFFNGVLLTPSADYKFVFDGDD
HSLIILFTKLEDEGEYTCMASNDYGKTICSAYLKINSKGEGHKDTETESAVAKSLEKLGGPC
PPHFLKELKPIRCAQGLPAIFEYTWGEPAPTVTWFKENKQLCTSVYYTIIHNPNGSGTFIVN
DPQREDSGLYICKAENMLGESTCAAELLVLLEDTDMTDTPCKAKSTPEAPEDFPQTPLKGP
AVEALDSEQEIATFVKDTILKAALITEENQQLSYEHIAKANELSSQLPLGAQELQSILEQDKL
TPESTREFLCINGSIHFQPLKEPSPNLQLQIVQSQKTFSKEGILMPEEPETQAVLSDTEKIFPSA MSIEQINSLTVEPLKTLLAEPEGNYPQSSIEPPMHSYLTSVAEEVLSPKEKTVSDTNREQRVT LQKQEAQSALILSQSLAEGHVESLQSPDVMISQWYEPLVPSEHSCTEGGKILIESANPLENA GQDSAVRIEEGKSLRFPLALEEKQVLLKEEHSDNVVMPPDQIIESKREPVAIKKVQEVQGRD LLSKESLLSGIPEEQRLNLKIQICRALQAAVASEQPGLFSEWLRNIEKVEVEAVNITQEPRHI MCMYLVTSAKSVTEEVTIIIED VDPQMANLKMELRDALCAIIYEEIDILTAEGPRIQQGAKTS
LQEEMDSFSGSQKVEPITEPEVESKYLISTEEVSYFNVQSRVKYLDATPVTKGVASAWSDE
KQDESLKPSEEKEESSSESGTEEVATVKIQEAEGGLIKEDGPMIHTPLVDTVSEEGDIVHLTT
SITNAKEVNWΎFENKLVPSDEKFKCLQDQNTYTLVIDKVNTEDHQGEYVCEALNDSGKTA
TSAKLTVVKRAAPVIKRKIEPLEVALGHLAKFTCEIQSAPNVRFQWFKAGREIYESDKCSIR
SSKYISSLEILRTQVVDCGEYTCKASNEYGSVSCTATLTVTEAYPPTFLSRPKSLTTFVGKAA
KFICTVTGTPVIETIWQKDGAALSPSPNWRISDAENKHILELSNLTIQDRGVYSCKASNKFG
ADICQAELIIIDKPHFIKELEPVQSAINKKVHLECQVDEDRKVTVTWSKDGQKLPPGKDYKI
CFEDKIATLEIPLAKLKDSGTYVCTASNEAGSSSCSATVTVREPPSFVKKVDPSYLMLPGES
ARLHCKLKGSPVIQVTWFKNNKELSESNTVRMYFVNSEAILDITDVKVEDSGSYSCEAVND
VGSDSCSTEIVIKEPPSFIKTLEPADIVRGTNALLQCEVSGTGPFEISWFKDKKQIRSSKKYRL
FSQKSLVCLEIFSFNSADVGEYECWANEVGKCGCMATHLLKEPPTFVKKVDDLIALGGQT
VTLQAA VRGSEPISVTWMKGQEVIREDGKIKMSFSNGV AVLIIPDVQISFGGKYTCLAENEA
GSQTSVGELΓVKEPAKIIERAELIQVTAGDPATLEYTVAGTPELKPKWYKDGRPLVASKKY
RISFKNNVAQLKFYSAELHDSGQYTFEISNEVGSSSCETTFTVLDRDIAPFFTKPLRNVDSW
NGTCRLDCKIAGSLPMRVSWFKDGKEIAASDRYRIAFVEGTASLEIIRVDMNDAGNFTCRA
TNSVGSKDSSGALΓVQEPPSFVTKPGSKDVLPGSAVCLKSTFQGSTPLTIRWFKGNKELVSG
GSCYITKEALESSLELYLVKTSDSGTYTCKVSNVAGGVECSANLFVKEPATFVEKLEPSQLL
KKGDATQLACKVTGTPPIKITWFANDREIKESSKHRMSFVESTAVLRLTDVGIEDSGEYMC
EAQNEAGSDHCSSTVIVKESPYFTKEFKPIEVLKEYDVMLLAEVAGTPPFEITWFKDNTILRS
GRKYKTFIQDHLVSLQILKFV AADAGEYQCRVTNEVGSSICSARVTLREPPSFIKKIESTSSL RGGTAAFQATLKGSLPITVTWLKDSDEITEDDNIRMTFENNVASLYLSGIEVKHDGKYVCQ AKNDAGIQRCSALLSVKEP ATITEEAVSIDVTQGDPATLQVKFSGTKEITAKWFKDGQELTL
GSKYKJSVTDTVSILKIISTEKXDSGEYTFEVQNDVGRSSCKARINVLDLIIPPSFTKKLKKM
DSIKGSFIDLECΓVAGSHPISIQWFKDDQEISASEKYKFSFHDNTAFLEISQLEGTDSGTYTCS
ATNKAGHNQCSGHLTVKEPPYFVEKPQSQDVNPNTRVQLKALVGGTAPMTIKWFKDNKE
LHSGAARSVWKDDTSTSLELFAAKATDSGTYICQLSNDVGTATSKATLFVKEPPQFIKKPSP
VLVLRNGQSTTFECQITGTPKIRVSWYLDGNEITAIQKHGISFIDGLATFQISGARVENSGTY
VCEARNDAGTASCSIELKVKEPPTFIRELKPVEVVKYSDVELECEVTGTPPFEVTWLKNNRE
IRSSKKYTLTDRVSVFNLHITKCDPSDTGEYQCIVSNEGGSCSCSTRVALKEPPSFIKKIENTT
WLKSSATFQSTVAGSPPISITWLKDDQILDEDDNVYISFVDSVATLQIRSVDNGHSGRYTC
QAKNESGVERCYAFLLVQEPAQIVEKAKSVDVTEKDPMTLECVVAGTPELKVKWLKDGK
QIVPSRYFSMSFENNVASFRIQSVMKQDSGQYTFKVENDFGSSSCDAYLRVLDQNIPPSFTK
KLTKMDKVLGSSIHMECKVSGSLPISAQWFKDGKEISTSAKYRLVCHERSVSLEVNNLELE
DTANYTCKVSNV AGDDACSGILTVKEPPSFLVKPGRQQAIPDSTVEFKAILKGTPPFKIKWF KDDVELVSGPKCFIGLEGSTSFLNLYSVDASKTGQYTCHVTNDVGSDSCTTMLLVTEPPKF VKKLEASKIVKAGDSSRLECKIAGSPEIRVVWFRNEHELP ASDKYRMTFIDSVAVIQMNNL STEDSGDFICEAQNPAGSTSCSTKVIVKEPPVFSSFPPIVETLKNAEVSLECELSGTPPFEVVW YKDKRQLRSSKKYKIASKNFHTSIHILNVDTSDIGEYHCKAQNEVGSDTCVCTVKLKEPPRF VSKLNSLTWAGEPAELQASIEGAQPIFVQWLKEKEEVIRESENIRITFVENVATLQFAKAEP ANAGKYICQIKNDGGMEENMATLMVLEPA VIVEKAGPMTVTVGETCTLECKV AGTPELSV EWYKDGKLLTSSQKHKFSFYNKISSLRILSVERQDAGTYTFQVQNNVGKSSCTA WDVSD RAVPPSFTRRLKNTGGVLGASCILECKVAGSSPISVA WFHEKTKIVSGAKYQTTFSDNVCTL
QLNSLDSSDMGNYTCVAANVAGSDECRAVLTVQEPPSFVKEPEPLEVLPGKNVTFTSVIRG
TPPFKVNWFRGARELVKGDRCNIYFEDTVAELELFNIDISQSGEYTCVVSNNAGQASCTTR
LFVKEPAAFLKRLSDHSVEPGKSΠLESTYTGTLPISVTWKKDGFNITTSEKCNΓVTTEKTCIL
EILNSTKRDAGQYSCEIENEAGRDVCGALVSTLEPPYFVTELEPLEAAVGDSVSLQCQVAG
TPEITVSWYKGDTKLRPTPEYRTYFTNNVATLVFNKVNINDSGEYTCKAENSIGTASSKTVF
RIQERQLPPSFARQLKDIEQTVGLPVTLTCRLNGSAPIQVCWYRDGVLLRDDENLQTSFVD NVATLKILQTDLSHSGQYSCSASNPLGTASSSARLTAREPKKSPFFDIKPVSIDVIAGESADF
ECHVTGAQPMRITWSKDNKEIRPGGNYTITCVGNTPHLRILKVGKGDSGQYTCQATNDVG
KDMCSAQLSVKEPPKFVKKLEASKV AKQGESIQLECKISGSPEIKVSWFRNDSELHESWKY
NMSFINSV ALLTINEASAEDSGDYICEAHNGVGDASCSTALTVKAPPVFTQKPSPVGALKGS
DVILQCEISGTPPFEVVWVKDRKQVRNSKKFKITSKHFDTSLFΠLNLEASDVGEYHCKATNE
VGSDTCSCSVKFKEPPRFVKKLSDTSTLIGDA VELRAIVEGFQPISVVWLKDRGEVIRESENT
RISFIDNIATLQLGSPEASNSGKYICQIKNDAGMRECSAVLTVLEPARIIEKPEPMTVTTGNPF
ALECVVTGTPELSAKWFKDGRELSADSKHHITFINKVASLKIPCAEMSDKGLYSFEVKNSV
GKSNCTVSVHVSDRIVPPSFIRKLKDVNAILGASWLECRVSGSAPISVGWFQDGNEΓVSGP
KCQSSFSENVCTLNLSLLEPSDTGIYTCVAANVAGSDECSAVLTVQEPPSFEQTPDSVEVLP
GMSLTFTSVIRGTPPFKVKWFKGSRELVPGESCNISLEDFVTELELFEVQPLESGDYSCLVTN
DAGSASCTTHLFVKEPATFVKRLADFSVETGSPIVLEATYTGTPPISVSWIKDEYLISQSERC
SITMTEKSTILEILESTIEDY AQYSCLIENEAGQDICEALVSVLEPPYFIEPLEHVEAVIGEP AT
LQCKVDGTPEIRISWYKEHTKLRS AP AYKMQFKNNV ASLVINKVDHSDVGEYSCKADNSV
GAVASSAVLVIKERKLPPFFARKLKDVHETLGFPVAFECRINGSEPLQVSWYKDGVLLKDD
ANLQTSFVHNVATLQILQTDQSHIGQYNCSASNPLGTASSSAKLILSEHEVPPFFDLKPVSV
DLALGESGTFKCHVTGTAPIKITWAKDNREIRPGGNYKMTLVENTATLTVLKVGKGDAGQ
YTCYASNIAGKDSCSAHLGVQEPPRFIKKLEPSRIVKQDEFTRYECKIGGSPEIKVLWYKDE
TEIQESSKFRMSFVDSVA VLEMHNLSVEDSGDYTCEAHNAAGSASSSTSLKVKEPPIFRKKP
HPΪETLKGADVHLECELQGTPPFHVSWYKDKRELRSGKKYKIMSENFLTSIHILNVDAADIG
EYQCKATNDVGSDTCVGSIALKAPPRFVKKLSDISTWGKEVQLQTTIEGAEPISVVWFKD
KGEIVRESDNIWISYSENIATLQFSRVEPANAGKYTCQIKNDAGMQECFATLSVLEPATΓVE
KPESIKVTTGDTCTLECTV AGTPELSTKWFKDGKELTSDNKYKISFFNKVSGLKIINVAPSDS
GVYSFEVQNPVGKDSCTASLQVSDRTVPPSFTRKLKETNGLSGSSWMECKVYGSPPISVS
WFHEGNEISSGRKYQTTLTDNTCALTVNMLEESDSGDYTCIATNMAGSDECSAPLTVREPP
SFVQKPDPMDVLTGTNVTFTSΓVKGTPPFSVSWFKGSSELVPGDRCNVSLEDSVAELELFDV
DTSQSGEYTCΓVSNEAGKASCTTHLYIKAPAKFVKRLNDYSIEKGKPLILEGTFTGTPPISVT
WKKNGINVTPSQRCNITTTEKSAILEIPSSTVEDAGQ YNCYIENASGKDS.CSAQILILEPPYFV
KQLEPVKVSVGDSASLQCQLAGTPEIGVSWYKGDTKLRPTTTYKMHFRNNVATLVFNQV
DINDSGEYICKAENSVGEVSASTFLTVQEQKLPPSFSRQLRDVQETVGLPWFDCAISGSEPI
SVSWYKDGKPLKDSPNVQTSFLDNTATLNIFKTDRSLAGQYSCTATNPIGSASSSARLILTE
GKNPPFFDIRLAPVDAVVGESADFECHVTGTQPIKVSWAKDSREIRSGGKYQISYLENSAHL
TVLKVDKGDSGQYTCYAVNEVGKDSCTAQLNIKERLIPPSFTKRLSETVEETEGNSFKLEG
RVAGSQPITVAWYKNNIEIQPTSNCEITFKNNTLVLQVRKAGMNDAGLYTCKVSNDAGSA
LCTSSIVIKEPKKPPVFDQHLTPVTVSEGEYVQLSCHVQGSEPIRIQWLKAGREIKPSDRCSF
SFASGTAVLELRDV AICADSGDYVCKASNVAGSDTTKSKVTIKDKPAVAPATKKAAVDGR
LFFVSEPQSIRVVEKTTATFIAKVGGDPIPNVKWTKGKWRQLNQGGRVFIHQKGDEAKLEI
RDTTKTDSGLYRCVAFNEHGEIESNVNLQVDERKKQEKIEGDLRAMLKKTPILKKGAGEEE
EIDIMELLKNVDPKEYEKYARMYGITDFRGLLQAFELLKQSQEEETHRLEIEEIERSERDEKE
FEELVSFIQQRLSQTEPVTLIKDIENQTVLKDNDAVFEIDIKINYPEIKLSWYKGTEKLEPSDK
FEISIDGDRHTLRVKNCQLKDQGNYRLVCGPHIASAKLTVIEPAWERHLQDVTLKEGQTCT
MTCQFSVPNVKSEWFRNGRILKPQGRHKTEVEHKVHKLTIADVRAEDQGQYTCKYEDLET
SAELRIEAEPIQFTKRIQNIVVSEHQSATFECEVSFDDAΓVTWYKGPTELTESQKYNFRNDGR
CHYMTIHNVTPDDEGVYSVIARLEPRGEARSTAELYLTTKEIKLELKPPDIPDSRVPIPTMPI
RA VPPEEIPPVVAPPIPLLLPTPEEKKPPPKRIEVTKKAVKKD AKKW AKPKEMTPREEIVKK
PPPPTTLIPAKAPEIIDVSSKAEEVKIMTITRKKEVQKEKEAVYEKKQAVHKEKRVFIESFEE
PYDELEVEPYTEPFEQPYYEEPDEDYEEIKVEAKKEVHEEWEEDFEEGQEYYEREEGYDEG
EEEWEEAYQEREVIQVQKEVYEESHERKVPAKVPEKKAPPPPKVIKKPVIEKIEKTSRRMEE
EKVQVTKVPEVSKKIVPQKPSRTPVQEEVIEVKVPAVHTKKMVISEEKMFFASHTEEEVSV
TVPEVQKEΓVTEEKIHVAVSKRVEPPPKVPELPEKPAPEEVAPVPIPKKVEPPAPKVPEVPKK
PVPEEKKPVPVPKKEPAAPPKVPEVPKKPVPEEKIPVPVAKKKEAPPAKVPEVQKRVVTEE
KITIVTQREESPPPAVPEIPKKKVPEERKPVPRKEEEVPPPPKVPALPKKPVPEEKVAVPVPV
AKKAPPPRAEVSKKTVVEEKRFVAEEKLSFAVPQRVEVTRHEVSAEEEWSYSEEEEGVSIS VYREEEREEEEEAEVTEYEVMEEPEEYVVEEKLHIISKRVEAEPAEVTERQEKKIVLKPKIP
AKIEEPPPAKVPEAPKKIVPEKKVPAPVPKKEKVPPPKVPEEPKKPVPEKKVPPKVIKMEEPL
PAKVTERHMQITQEEKVLVAVTKKEAPPKARVPEEPKRAVPEEKVLKLKPKREEEPPAKVT
EFRKRVVKEEKVSIEAPKREPQPIKEVTIMEEKERAYTLEEEAVSVQREEEYEEYEEYDYKE
FEEYEPTEEYDQYEEYEEREYERYEEHEEYITEPEKPIPVKPVPEEPVPTKPKAPPAKVLKKA
VPEEKVPVPIPKKLKPPPPKVPEEPKKVFEEKIRISITKREKEQVTEPAAKVPMKPKRVVAEE
KVPVPRKEVAPPVRVPEVPKELEPEEVAFEEEWTHVEEYLVEEEEEYIHEEEEFITEEEVVP
VIP VKVPEVPRKPVPEEKKP VPVPKKKEAPPAKVPEVPKKPEEKVPVLIPKKEKPPPAKVPE
VPKKPVPEEKVPVPVPKKVEAPPAKVPEVPKKPVPEKKVPVPAPKKVEAPPAKVPEVPKKL
IPEEKKPTPVPKKVEAPPPKVPKKREPVPVPVALPQEEEVLFEEEΓVPEEEVLPEEEEVLPEEE
EVLPEEEEVLPEEEEIPPEEEEVPPEEEYVPEEEEFVPEEEVLPEVKPKVPVPAPVPEIKKKVT
EKKVVIPKKEEAPPAKVPEVPKKVEEKRIILPKEEEVLPVEVTEEPEEEPISEEEIPEEPPSIEE
VEEVAPPRVPEVIKKAVPEAPTPVPKKVEAPPAKVSKKIPEEKVPVPVQKKEAPPAKVPEVP
KKVPEKKVLVPKKEAVPPAKGRTVLEEKVSVAFRQEVWKERLELEVVEAEVEEIPEEEEF
HEVEEYFEEGEFHEVEEFIKLEQHRVEEEHRVEKVHRVIEVFEAEEVEVFEKPKAPPKGPEIS
EKIIPPKKPPTKVVPRKEPPAKVPEVPKKIVVEEKVRVPEEPRVPPTKVPDVLPPKEVVPEKK
VPVPPAKKPEAPPPKVPEAPKEWPEKKVPVPPPKKPEVPPTKVPEVPKAAVPEKKVPEAIP
PKPESPPPEVPEAPKEWPEKKVPAAPPKKPEVTPVKVPEAPKEWPEKKVPVPPPKKPEVP
PTKVPEVPKVAVPEKKVPEAIPPKPESPPPEVFEEPEEVALEEPPAEWEEPEPAAPPQVTVP
PKKPVPEKKAPAVVAKKPELPPVKVPEVPKEVVPEKKVPLVVPKKPEAPPAKVPEVPKEVV
PEKKVAVPKKPEVPPAKVPEVPKKPVLEEKPAVPVPERAESPPPEVYEEPEEIAPEEEIAPEE
EKPVPVAEEEEPEVPPPAVPEEPKKIIPEKKVPVIKKPEAPPPKEPEPEKVIEKPKLKPRPPPPP
PAPPKEDVKEKIFQLKAIPKKKVPEKPQVPEKVELTPLKVPGGEKKVRKLLPERKPEPKEEV
VLKSVLRKRPEEEEPKVEPKKLEKVKKPA VPEPPPPKPVEEVEVPTVTKRERKIPEPTKVPEI KPAIPLPAPEPKPKPEAEVKTIKPPPVEPEPTPIAAPVTVPVVGKKAEAKAPKEEAAKPKGPI KGVPKKTPSPIEAERRKLRPGSGGEKPPDEAPFTYQLKAVPLKFVKEIKDIILTESEFVGSSAI FECLVSPSTAITTWMKDGSNIRESPKHRFIADGKDRKLHIIDVQLSDAGEYTCVLRLGNKEK TSTAKLWEELPVRFVKTLEEEVTVVKGQPLYLSCELNKERDW WRKDGKIVVEKPGRIVP
GVIGLMRALTINDADDTDAGTYTVTVENANNLECSSCVKWEVIRD WLVKPIRDQHVKPK
GTAIFACDIAKDTPNIKWFKGYDEIPAEPNDKTEILRDGNHLYLKIKNAMPEDIAEYAVEIE
GKRYPAKLTLGEREVELLKPIEDVTIYEKESASFDAEISEADIPGQWKLKGELLRPSPTCEIK
AEGGKRFLTLHKVKLDQAGEVLYQALNAITTAILTVKEIELDFAVPLKDVTVPERRQARFE
CVLTREANVIWSKGPDIIKSSDKFDIIADGKKHILVINDSQFDDEGVYTAEVEGKKTSARLF
VTGIRLKFMSPLEDQTVKEGETATFVCELSHEKMHVVWFKNDAKLHTSRTVLISSEGKTH
KLEMKEVTLDDISQIKAQVKELSSTAQLKVLEADPYFTVKLHDKTA VEKDEITLKCEVSKD
VPVKWFKDGEEIVPSPKYSIKADGLRRILKIKKADLKDKGEYVCDCGTDKTKANVTVEAR
LIKVEKPLYGVEVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDCEIIEDGKKHILILHNC
QLGMTGEVSFQAANAKSAANLKVKELPLIFITPLSDVKVFEKDEAKFECEVSREPKTFRWL
KGTQEITGDDRPELIKDGTKHSMVIKSAAFEDEAKYMFEAEDKHTSGKLΠEGIRLKFLTPL
KDVTAKEKESAVFTVELSHDNIRVKWFKNDQRLHTTRSVSMQDEGKTHSITFKDLSIDDTS
QIRVEAMGMSSEAKLTVLEGDPYFTGKLQDYTGVEKDEVILQCEISKADAPVKWFKDGKE
IKPSKNAVIKADGKKRMLILKKALKSDIGQYTCDCGTDKTSGKLDIEDREIKLVRPLHSVEV
METETARFETEISEDDIHANWKLKGEALLQTPDCEIKEEGKIHSLVLHNCRLDQTGGVDFQ
AANVKSSAHLRVKPRVIGLLRPLKDVTVTAGETATFDCELSYEDIPVEWYLKGKKLEPSDK
WPRSEGKVHTLTLRDVKLEDAGEVQLTAKDFKTHANLFVKEPPVEFTKPLEDQTVEEGA
TA VLECEVSRENAKVK WFKNGTEILKSKKYEΓVADGRVRKLVIHDCTPEDIKTYTCDAKDF
KTSCNLNWPPHVEFLRPLTDLQVREKEMARFECELSRENAKVKWFKDGAEIKKGKKYDII
SKGAVRILVINKCLLDDEAEYSCEVRTARTSGMLTVLEEEAVFTKNLANIEVSETDTIKLVC
EVSKPGAEVIWYKGDEEIIETGRYEILTEGRKRILVIQNAHLEDAGNYNCPVLPSSRTDGKVK
VHELAAEFISKPQNLEILEGEKAEFVCSISKESFPVQWKRDDKTLESGDKYDVIADGKKRVL
WKDATLQDMGTYWMVGAARAAAHLTVIEKLRIVVPLKDTRVKEQQEWFNCEVNTEG
AKAKWFRNEEAIFDSSKYIILQKDLVYTLRIRDAHLDDQANYNVSLTNHRGENVKSAANLI
VEEEDLRWEPLKDIETMEKKSVTFWCKVNRLNVTLKWTKNGEEVPFDNRVSYRVDKYKH MLTIKDCGFPDEGEYIVTAGQDKSVAELLIIEAPTEFVEHLEDQTVTEFDDAVFSCQLSREK
ANVKWYRNGREIKEGKKYKFEKDGSIHRLIIKDCRLDDECEYACGVEDRKSRARLFVEEIP
VEIIRPPQDILEAPGADVVFLAELNKDKVEVQWLRNNMWVQGDKHQMMSEGKIHRLQIC
DIKPRDQGEYRFIAKDKEARAKLELAAAPKIKTADQDLVVDVGKPLTMWPYDAYPKAEA
EWFKENEPLSTKTIDTTAEQTSFRILEAKKGDKGRYKIVLQNKHGKAEGFINLKVIDVPGPV
RNLEVTETFDGEVSLAWEEPLTDGGSKIIGYVVERRDIKRKTWVLATDRAESCEFTVTGLQ
KGGVEYLFRVSARNRVGTGEPVETDNPVEARSKYDVPGPPLNVTITDVNRFGVSLTWEPPE
YDGGAEITNYVIELRDKTSIRWDTAMTVRAEDLSATVTDVVEGQEYSFRVRAQNRIGVGK
PSAATPFVKV ADPIERPSPPVNLTSSDQTQSSVQLKWEPPLKDGGSPILGYIIERCEEGKDNW
IRCNMKLVPELTYKVTGLEKGNKYLYRVSAENKAGVSDPSEILGPLTADDAFVEPTMDLS
AFKDGLEVΓVPNPITILVPSTGYPRPTATWCFGDKVLETGDRVKMKTLSAYAELVISPSERS
DKGIYTLKLENRVKTISGEIDVNVIARPSAPKELKFGDITKDSVHLTWEPPDDDGGSPLTGY
WEKREVSRKTWTKVMDFVTDLEFTVPDLVQGKEYLFKVCARNKCGPGEPAYVDEPVNM
STPATVPDPPENVKWRDRTANSIFLTWDPPKNDGGSRIKGYΓVERCPRGSDKWVACGEPVA
ETKMEVTGLEEGKWYAYRVKALNRQGASKPSRPTEEIQAVDTQEAPEIFLDVKLLAGLTV
KAGTKiELP ATVTGKPEPKITWTKADMILKQDKRITIENVPKKSTVTΓVDSKRSDTGTYΠEA
VNVCGRATA WEVNVLDKPGPPAAFDITDVTNESCLLTWNPPRDDGGSKITNYWERRAT
DSEVWHKLSSTVKDTNFKATKLIPNKEYIFRVAAENMYGVGEPVQASPITAKYQFDPPGPP
TRLEPSDITKDAVTLTWCEPDDDGGSPITGYWVERLDPDTDKWVRCNKMPVKDTTYRVK
GLTNKKKYRFRVLAENLAGPGKPSKSTEPILIKDPIDPPWPPGKPTVKDVGKTSVRLNWTK
PEHDGGAKIESYVIEMLKTGTDEWVRVAEGVPTTQHLLPGLMEGQEYSFRVRAVNKAGES
EPSEPSDPVLCREKXYPPSPPRWLEVINITKNTADLKWTVPEKDGGSPITNYΓVEKRDVRRK
GWQTVDTTVKDTKCTVTPLTEGSLYVFRVAAENAIGQSDYTEIEDSVLAKDTFTTPGPPYA
LAVVDVTKRHVDLKWEPPKNDGGRPIQRYVIEKKERLGTRWVKAGKTAGPDCNFRVTDV
IEGTEVQFQVRAENEAGVGHPSEPTEILSIEDPTSPPSPPLDLHVTDAGRKHIAIAWKPPEKN
GGSPIIGYHVEMCPVGTEKWMRVNSRPIKDLKFKVEEGVVPDKEYVLRVRAVNAIGVSEPS
EISENWAKDPDCKPTIDLETHDIIVIEGEKLSIPVPFRAVPVPTVSWHKDGKEVKASDRLTM
KNDHISAHLEVPKSVRADAGIYTITLENKLGSATASINVKVIGLPGPCKDIKASDITKSSCKL
TWEPPEFDGGTPILHYVLERREAGRRTYIPVMSGENKLSWTVKDLIPNGEYFFRVKAVNKV
GGGEYIELKNPVIAQDPKQPPDPPVDVEVHNPTAEAMTITWKPPLYDGGSKIMGYIIEKIAK
GEERWKRCNEHLVPILTYTAKGLEEGKEYQFRVRAENAAGISEPSRATPPTKAVDPIDAPK
VILRTSLEVKRGDEIALDASISGSPYPTITWIKDENVIVPEEIKKRAAPLVRRRKGEVQEEEPF
VLPLTQRLSIDNSKKGESQLRVRDSLRPDHGLYMIKVENDHGIAKAPCTVSVLDTPGPPINF
VFEDIRKTSVLCKWEPPLDDGGSEIINYTLEKKDKTKPDSEWIVVTSTLRHCKYSVTKLIEG
KEYLFRVRAENRFGPGPPCVSKPLVAKDPFGPPDAPDKPIVEDVTSNSMLVKWNEPKDNGS
PILGYWLEKREVNSTHWSRVNKSLLNALKANVDGLLEGLTYVFRVCAENAAGPGKFSPPS
DPKTAHDPISPPGPPIPRVTDTSSTTIELEWEPPAFNGGGEIVGYFVDKQLVGTNEWSRCTEK
MIKVRQYTVKEIREGADYKLRVSAVNAAGEGPPGETQPVTVAEPQEPPAVELDVSVKGGI
QIMAGKTLRIPAVVTGRPVPTKVWTKEEGELDKDRVVIDNVGTKSELIIKDALRKDHGRYV
ITATNSCGSKFAAARVEVFDVPGPVLDLKPVVTNRKMCLLNWSDPEDDGGSEITGFIIERK
DAKMHTWRQPIETERSKCDITGLLEGQEYKFRVIAKNKFGCGPPVEIGPILAVDPLGPPTSPE
RLTYTERTKSTITLDWKEPRSNGGSPIQGYIIEKRRHDKPDFERVNKRLCPTTSFLVENLDEH
QMYEFRVKAVNEIGESEPSLPLNVVIQDDEVPPTIKLRLSVRGDTIKVKAGEPVHIPADVTG
LPMPKIEWSKNETVIEKPTDALQITKEEVSRSEAKTELSIPKAVREDKGTYTVTASNRLGSV
FRNVHVEVYDRPSPPRNLAVTDIKAESCYLTWDAPLDNGGSEITHYVIDKRDASRKKAEW
EEVTNTAVEKRYGIWKLIPNGQYEFRVRAVNKYGISDECKSDKVVIQDPYRLPGPPGKPKV
LARTKGSMLVSWTPPLDNGGSPITGYWLEKREEGSPYWSRVSRAPITKVGLKGVEFNVPRL
LEGVKYQFRAMAINAAGIGPPSEPSDPEVAGDPIFPPGPPSCPEVKDKTKSSISLGWKPPAKD
GGSPIKGYIVEMQEEGTTDWKRVNEPDKLITTCECWPNLKELRKYRFRVKAVNEAGESEP
SDTTGEIP ATDIQEEPEVFIDIGAQDCLVCKAGSQIRIPA VIKGRPTPKSSWEFDGKAKKAMK
DGVHDIPEDAQLETAENSSVIIIPECKRSHTGKYSITAKNKAGQKTANCRVKVMDVPGPPK
DLKVSDITRGSCRLSWKMPDDDGGDRIKGYVIEKRTIDGKAWTKVNPDCGSTTFVVPDLL
SEQQYFFRVRAENRFGIGPPVETIQRTTARDPIYPPDPPIKLKIGLITKNTVHLSWKPPKNDG GSPVTHYIVECLAWDPTGTKKEAWRQCNKRDVEELQFTVEDLVEGGEYEFRVKAVNAAG
VSKPSATVGPCDCQRPDMPPSIDLKEFMEVEEGTNVNΓVAKIKGVPFPTLTWFKAPPKKPD
NKEPVLYDTHVNKLWDDTCTLVIPQSRRSDTGLYTITAVNNLGTASKEMRLNVLGRPGPP
VGPIKFESVSADQMTLSWFPPKDDGGSKITNYVIEKREANRKTWVHVSSEPKECTYTIPKLL
EGHEYVFRIMAQNKYGIGEPLDSEPETARNLFSVPGAPDKPTVSSVTRNSMTVNWEEPEYD
GGSPVTGYWLEMKDTTSKRWKRVNRDPIKAMTLGVSYKVTGLIEGSDYQFRVYAINAAG
VGPASLPSDPATARDPIAPPGPPFPKVTD WTKSSADLEWSPPLKDGGSKVTGYIVEYKEEG
KEEWEKGKDKEVRGTKLWTGLKEGAFYKFRVSAVNIAGIGEPGEVTDVIEMKDRLVSPD
LQLDASVRDRIVVHAGGVIRIIAYVSGKPPPTVTWNMNERTLPQEATIETTAISSSMVIKNC
QRSHQGVYSLLAKNEAGERKKTIIVDVLDVPGPVGTPFLAHNLTNESCKLTWFSPEDDGGS
PITNYVIEKRESDRRAWTPVTYTVTRQNATVQGLIQGKAYFFRIAAENSIGMGPFVETSEAL
VIREPITVPERPEDLEVKEVTKNTVTLTWNPPKYDGGSEIINYVLESRLIGTEKFHKVTNDNL
LSRKYTVKGLKEGDTYEYRVSAVNIVGQGKPSFCTKPITCKDELAPPTLHLDFRDKLTIRVG
EAFALTGRYSGKPKPKVSWFKDEADVLEDDRTHIKTTPATLALEKIKAKRSDSGKYCVVV
ENSTGSRKGFCQVNVVDRPGPPVGPVSFDEVTKDYMVISWKPPLDDGGSKITNYIIEKKEV
GKDVWMPVTSASAKTTCKVSKLLEGKDYIFRIHAENLYGISDPLVSDSMKAKDRFRVPDA
PDQPΓVTEVTKDSALVTWNKPHDGGKPITNYILEKRETMSKRWARVTKDPIHPYTKFRVPD
LLEGCQYEFRVSAENEIGIGDPSPPSKPVFAKDPIAKPSPPVNPEAIDTTCNSVDLTWQPPRH
DGGSKILGYIVEYQKVGDEEWRRANHTPESCPETKYKVTGLRDGQTYKFRVLAVNAAGES
DPAHVPEPVLVKDRLEPPELILDANMAREQHIKVGDTLRLSAIIKGVPFPKVTWKKEDRDA
PTKARIDVTPVGSKLEIRNAAHEDGGIYSLTVENPAGSKTVSVKVLVLDKPGPPRDLEVSEI
RKDSCYLTWKEPLDDGGSVITNYVVERRDVASAQWSPLSATSKKKSHFAKHLNEGNQYLF
RVAAENQYGRGPFVETPKPIKALDPLHPPGPPKDLHHVDVDKTEVSLVWNKPDRDGGSPIT
GYLVEYQEEGTQD WIKFKTVTNLECWTGLQQGKTYRFRVKAENΓVGLGLPDTTIPIECQE
KLVPPSVELDVKLIEGLWKAGTTVRFPAIIRGVPVPTAKWTTDGSEIKTDEHYTVETDNFS
SVLTIKNCLRRDTGEYQITVSNAAGSKTVAVHLTVLDVPGPPTGPINILDVTPEHMTISWQP
PKI)DGGSPVINYIVEKQDTRKDTWGVVSSGSSKTKLKIPHLQKGCEYVFRVRAENKIGVGP
PLDSTPTVAKHKFSPPSPPGKPWTDITENAATVSWTLPKSDGGSPITGYYMERREVTGKW
VRVNKTPIADLKFRVTGLYEGNTYEFRVFAENLAGLSKPSPSSDPIKACRPIKPPGPPINPKL
KDKSRETADLVWTKPLSDGGSPILGYVVECQKPGTAQWNRINKDELIRQCAFRVPGLIEGN
EYRFRIKAANIVGEGEPRELAESVIAKDILHPPEVELDVTCRDVITVRVGQTIRILARVKGRP
EPDiTWTKEGKVLVREKRVDLiQDLPRVELQiKEA VRADHGKYIISAKNSSGHAQGSAΓVN
VLDRPGPCQNLKVTNVTKENCTISWENPLDNGGSEITNFΓVEYRKPNQKGWSIVASDVTKR
LIKANLLANNEYYFRVCAENKVGVGPTIETKTPILAINPIDRPGEPENLHIADKGKTFVYLK
WRRPDYDGGSPNLSYHVERRLKGSDDWERVHKGSIKETHYMVDRCVENQIYEFRVQTKN
EGGESDWVKTEEVWKEDLQKPVLDLKLSGVLTVKAGDTIRLEAGVRGKPFPEVAWTKD
KDATDLTRSPRVKIDTRADSSKFSLTKAKRSDGGKYWTATNTAGSFVAYATVNVLDKPG
PVRNLKΓVDVSSDRCTVCWDPPEDDGGCEIQNYILEKCETKRMVWSTYSATVLTPGTTVTR
LIEGNEYIFRVRAENKIGTGPPTESKPVIAKTKYDKPGRPDPPEVTKVSKEEMTWWNPPEY
DGGKSITGYFLEKKEKHSTRWVPVNKSAIPERRMKVQNLLPDHEYQFRVKAENEIGIGEPS
LPSRPVVAKDPIEPPGPPTNFRWDTTKHSITLGWGKPVYDGGAPIIGYVVEMRPKIADASP
DEGWKRCNAAAQLVRKEFTVTSLDENQEYEFRVCAQNQVGIGRP AELKEAIKPKEILEPPE
IDLDASMRKLVΓVRAGCPIRLFAIVRGRPAPKVTWRKVGIDNWRKGQVDLVDTMAFLVIP
NSTRDDSGKYSLTLVNPAGEKAVFVNVRVLDTPGPVSDLKVSDVTKTSCHVSWAPPENDG
GSQVTHYIVEKREADRKTWSTVTPEVKKTSFHVTNLVPGNEYYFRVTAVNEYGPGVPTDV
PKPVLASDPLSEPDPPRKLEVTEMTKNSATLAWLPPLRDGGAKIDGYITSYREEEQPADRW
TEYSVVKDLSLWTGLKEGKKYKFRVAARNAVGVSLPREAEGVYEAKEQLLPPKILMPEQ
ITIKAGKKLRFFIAFR^YGKPHPTCKWKKGEDEVVTSSHLAVHKADSSSILIIKDVTRKDSGYY
SLTAENSSGTDTQKIKVWMDAPGPPQPPFDISDIDADACSLSWHIPLEDGGSNITNYΓVEKC
DVSRGDWVTALASVTKTSCRVGKLIPGQEYIFRVRAENRFGISEPLTSPKMVAQFPFGVPSE
PKNARVTKVNKDCIFVAWDRPDSDGGSPIIGYLIERKERNSLLWVKANDTLVRSTEYPCAG
LVEGLEYSFRIYALNKAGSSPPSKPTEYVTARMPVDPPGKPEVIDVTKSTVSLIWARPKHDG
GSKIIGYFVEACKLPGDKWVRCNTAPHQIPQEEYTATGLEEKAQYQFRAIARTAVNISPPSE PSDPVTILAENVPPRIDLSVAMKSLLTVKAGTNVCLDATVFGKPMPTVSWKKDGTLLKPAE
GIKMAMQRNLCTLELFSVNRKDSGDYTITAENSSGSKSATIKLKVLDKPGPPASVKINKMY
SDRAMLSWEPPLEDGGSEITNYIVDKRETSPVPNWAQVSATVPITSCSVEKLIEGHEYQFRIC
AENKYGVGDPVFTEPAIAKNPYDPPGRCDPPVISNITKDHMTVSWKPPADDGGSPITGYLL
EKRETQAVNWTKVNRKPIIERTLKATGLQEGTEYEFRVTAINKAGPGKPSDASKAAYARDP
QYPP APP AFPKVYDTTRSSVSLSWGKP AYDGGSPIIGYLVEVKRADSDNWVRCNLPQNLQ
KTRFEVTGLMEDTQYQFRVYAVNKIGYSDPSDVPDKHYPKDILIPPEGELDADLRKTLILRA
GVTMRLYVPVKGRPPPKITWSKPNVNLRDRIGLDIKSTDFDTFLRCENVNKYDAGKYILTL
ENSCGKKEYTΓVVKVLDTPGPPVNVTVKEISKDSAYVTWEPPΠDGGSPIINYVVQKRDAER
KSWSTVTTECSKTSFRVANLEEGKSYFFRVFAENEYGIGDPGETRDAVKASQTPGPVVDLK
VRSVSKSSCSIGWKKPHSDGGSWIGYVVDFLTEENKWQRVMKSLSLQYSAKDLTEGKEYT
FRVSAENENGEGTPSEITWARDDVVAPDLDLKGLPDLCYLAKENSNFRLKIPIKGKPAPSV
SWKKGEDPLATDTRVSVESSAVNTTLIVYDCQKSDAGKYTITLKNVAGTKEGTISIKVVGK
PGIPTGPIKFDEVTAEAMTLKWAPPKDDGGSEITNYILEKRDSVNNKWVTCASAVQKTTFR
VTRLHEGMEYTFRVSAENKYGVGEGLKSEPIVARHPFDVPDAPPPPNIVDVRHDSVSLTWT
DPKKTGGSPITGYHLEFKERNSLLWKRANKTPIRMRDFKVTGLTEGLEYEFRVMAINLAGV
GKPSLPSEPVVALDPIDPPGKPEVINITRNSVTLIWTEPKYDGGHKLTGYIVEKRDLPSKSW
MKANHVNVPECAFTVTDLVEGGKYEFRIRAKNTAGAISAPSESTETIICKDEYEAPTΓVLDP
TIKDGLTIKAGDTΓVLNAISILGKPLPKSSWSKAGKDIRPSDITQITSTPTSSMLTIKYATRKD
AGEYTITATNPFGTKVEHVKVTVLDVPGPPGPVEISNVSAEKATLTWTPPLEDGGSPIKSYIL
EKRETSRLLWTWSEDIQSCRHVATKLIQGNEYIFRVSAVNHYGKGEPVQSEPVKMVDRFG
PPGPPEKPEVSNVTKNTATVSWKRPVDDGGSEITGYHVERREKKSLRWVRAIKTPVSDLRC
KVTGLQEGSTYEFRVSAENRAGIGPPSEASDSVLMKDAAYPPGPPSNPHVTDTTKKSASLA
WGKPHYDGGLEITGYVVEHQKVGDEAWIKDTTGTALRITQFVVPDLQTKEKYNFRISAIN
DAGVGEP AVIPDVEΓVEREMAPDFELDAELRRTLVVRAGLSIRIFVPIKGRP APEVTWTKDN
INLKNRANIENTESFTLLIIPECNRYDTGKFVMTIENPAGKKSGFVNVRVLDTPGPVLNLRPT
DITKDSVTLHWDLPLIDGGSMTNYIVEKREATRKSYSTATTKCHKCTYKVTGLSEGCEYFF
RVMAENEYGIGEPTETTEPVKASEAPSPPDSLNIMDITKSTVSLAWPKPKHDGGSKITGYVI
EAQRKGSDQWTHITTVKGLECVVRNLTEGEEYTFQVMAVNSAGRSAPRESRPVΓVKEQTM
LPELDLRGIYQKLVIAKAGDNIKVEIPVLGRPKPTVTWKKGDQILKQTQRVNFETTATSTIL
NINECVRSDSGPYPLTARNIVGEVGDVITIQVHDIPGPPTGPIKFDEVSSDFVTFSWDPPEND
GGVPISNYVVEMRQTDSTTWVELATTVIRTTYKATRLTTGLEYQFRVKAQNRYGVGPGITS
ACIVANYPFKVPGPPGTPQVTA VTKDSMTISWHEPLSDGGSPILGYHVERKERNGIL WQTV
SKALVPGNIFKSSGLTDGIAYEFRVIAENMAGKSKPSKPSEPMLALDPIDPPGKPVPLNITRH
TVTLKWAKPEYTGGFKITSYIVEKRDLPNGRWLKANFSNILENEFTVSGLTEDAAYEFRVIA
KNAAGAISPPSEPSDAITCRDDVEAPKIKVDVKFKDTVILKAGEAFRLEADVSGRPPPTMEW
SKDGKELEGTAKLEIKIADFSTNLVNKDSTRRDSGAYTLTATNPGGFAKHIFNVKVLDRPG
PPEGPLAVTEVTSEKCVLSWFPPLDDGGAKIDHYIVQKRETSRLAWTNVASEVQVTKLKVT
KLLKGNEYIFRVMAVNKYGVGEPLESEPVLAVNPYGPPDPPKNPEVTTITKDSMVVCWGH
PDSDGGSEIINYΓVERRDKAGQRWIKCNKKTLTDLRYKVSGLTEGHEYEFRIMAENAAGIS
APSPTSPFYKACDTVFKPGPPGNPRVLDTSRSSISIAWNKPIYDGGSEITGYMVEIALPEEDE
WQIVTPPAGLKATSYTITGLTENQEYKIRIYAMNSEGLGEPALVPGTPKAEDRMLPPEIELD
ADLRKVVTiRAccTLRLFVPiKGRP APEVKWARDHGESLDKASIESTSSYTLLΓVGNVNRFD
SGKYILTVENSSGSKSAFVNVRVLDTPGPPQDLKVKEVTKTSVTLTWDPPLLDGGSKIKNYI
VEKRESTRKAYSTVATNCHKTSWKVDQLQEGCSYYFRVLAENEYGIGLPAETAESVKASE
RPLPPGKITLMDVTRNSVSLSWEKPEHDGGSRILGYIVEMQTKGSDKWATCATVKVTEATI
TGLIQGEEYSFRVSAQNEKGISDPRQLSVPVIAKDLVIPPAFKLLFNTFTVLAGEDLKVDVPF
IGRPTPAVTWHKDNVPLKQTTRVNAESTENNSLLTIKDACREDVGHYVVKLTNSAGEAIET
LNVIVLDKPGPPTGPVKMDEVTADSITLSWGPPKYDGGSSINNYΓVEKRDTSTTTWQΓVSAT
VARTTIKACRLKTGCEYQFRIAAENRYGKSTYLNSEPTVAQYPFKVPGPPGTPVVTLSSRDS
MEVQWNEPISDGGSRVIGYHLERKERNSILWVKLNKTPIPQTKFKTTGLEEGVEYEFRVSA
ENIVGIGKPSKVSECYVARDPCDPPGRPEAIIVTRNSVTLQ WKKPTYDGGSKITGYIVEKKE
LPEGRWMKASFTNIIDTHFEVTGLVEDHRYEFRVIARNAAGVFSEPSESTGAITARDEVDPP WSMDPKYKDTIVVHAGESFKVDADIYGKPIPTIQWIKGDQELSNTARLEIKSTDFATSLSVK
DAVRVDSGNYILKAKNVAGERSVTVNVKVLDRPGPPEGPVVISGVTAEKCTLAWKPPLQD
GGSDIINYIVERRETSRLVWTVVDATSFVQTLSCKVTKLLEGNEYTFRIMAVNKYGVGEPLES
EPVVAKNPFWPDAPKAPEVTTVTKDSMΓVVWERPASDGGSEILGYVLEKRDKEGIRWTR
CHKRLIGELRLRVTGLIENHDYEFRVSAENAAGLSEPSPPSAYQKACDPIYKPGPPNNPKVI
DITRSSVFLSWSKPIYDGGCEIQGYΓVEKCDVSVGEWTMCTPPTGINKTNIEVEKLLEKHEY
NFRICAINKAGVGEHADVPGPIIVEEKLEAPDIDLDLELRKIINIRAGGSLRLFVPIKGRPTPE
VKWGKVDGEIRDAAIIDVTSSFTSLVLDNVNRYDSGKYTLTLENSSGTKSAFVTVRVLDTP
SPPVNLKVTEITKDSVSITWEPPLLDGGSKIKNYIVEKREATRKSYAAVVTNCHKNSWKIDQ
LQEGCSYYFRVTAENEYGIGLPAQTADPIKVAEVPQPPGKITVDDVTRNSVSLSWTKPEHD
GGSKIIQYIVEMQAKHSEKWSECARVKSLQAVITNLTQGEEYLFRWAVNEKGRSDPRSLA
VPIV AKDLVIEPDVKPAFSSYSVQVGQDLKIEVPISGRPKPTIT WTKDGLPLKQTTRINVTDS
LDLTTLSIKETHKDDGGQYGITVANWGQKTASIEIVTLDKPDPPKGPVKFDDVSAESITLS
WNPPLYTGGCQITNYΓVQKRDTTTTVWDWSATVARTTLKVTKLKTGTEYQFRIFAENRY
GQSF ALESDPIV AQYPYKEPGPPGTPF ATAISKDSMVIQWHEPVNNGGSPVIGYHLERKERN
SILWTKVNKTIIHDTQFKAQNLEEGIEYEFRVYAENΓVGVGKASKNSECYV ARDPCDPPGTP
EPIMVKRNEITLQWTKPVYDGGS-MITGYIVEKRDLPDGRWMKASFTNVIETQFTVSGLTED
QRYEFRVIAKNAAGAISKPSDSTGPITAKDEVELPRISMDPKFRDTIVVNAGETFRLEADVH
GKPLPTIEWLRGDKEIEESARCEIKNTDFKALLIVKDAIRIDGGQYILRASNVAGSKSFPVNV
KVLDRPGPPEGPVQVTGVTSEKCSLTWSPPLQDGGSDISHYVVEKRETSRLAWTWASEV
VTNSLKVTKLLEGNEYVFRIMAVNKYGVGEPLESAPVLMKNPFVLPGPPKSLEVTNIAKDS
MTVCWNRPDSDGGSEIIGYΓVEKRDRSGIRWIKCNKRRITDLRLRVTGLTEDHEYEFRVSAE
NAAGVGEPSPATVYYKACDPVFKPGPPTNAHFVDTTKNSITLAWGKPΓYDGGSEILGYVVEI
CKADEEEWQIVTPQTGLRVTRFEISKLTEHQEYKIRVCALNKVGLGEATSVPGTVKPEDKL
EAPELDLDSELRKGIVVRAGGSARIHIPFKGRPTPEITWSREEGEFTDKVQIEKGVNYTQLSI
DNCDRNDAGKYILKLENSSGSKSAFVTVKVLDTPGPPQNLAVKEVRKDSAFLVWEPPIIDG
GAKVKNYVIDKRESTRKAYANVSSKCSKTSFKVENLTEGAIYYFRVMAENEFGVGVPVET
VDAVKAAEPPSPPGKVTLTDVSQTSASLMWEKPEHDGGSRVLGYVVEMQPKGTEKWSΓV
AESKVCNAVVTGLSSGQEYQFRVKAYNEKGKSDPRVLGVPVIAKDLTIQPSLKLPFNTYSI
QAGEDLKIEIPVIGRPRPNISWVKDGEPLKQTTRVNVEETATSTVLHIKEGNKDDFGKYTVT
ATNSAGTATENLSVΓVLEKPGPPVGPVRFDEVSADFVVISWEPP AYTGGCQISNYIVEKRDT
TTTTWHMVSATVARTTIKITKLKTGTEYQFRIFAENRYGKSAPLDSKAVIVQYPFKEPGPPG
TPFVTSISKDQMLVQWHΈPVNDGGTKΠGYHLEQKEKNSILWVKLNKTPIQDTKFKTTGLD
EGLEYEFKVSAENIVGIGKPSKVSECFVARDPCDPPGRPEAIVITRNNVTLKWKKPAYDGGS
KITGYIVEKKDLPDGRWMKASFTNVLETEFTVSGLVEDQRYEFRVIARNAAGNFSEPSDSS
GAITARDEIDAPNASLDPKYKDVIVVHAGETFVLEADIRGKPIPDVVWSKDGKELEETAAR
MEIKSTIQKTTLVVKDCIRTDGGQYILKLSNVGGTKS.IPITVKVLDRPGPPEGPLKVTGVTAE
KCYLAWNPPLQDGGANISHYIIEKRETSRLSWTQVSTEVQALNYKVTKLLPGNEYIFRVMA
VNKYGIGEPLESGPVTACNPYKPPGPPSTPEVSAITKDSMWTWARPVDDGGTEIEGYILEK
RDKEGVRWTKCNKKTLTDLRLRVTGLTEGHSYEFRVAAENAAGVGEPSEPSVFYRACDA
LYPPGPPSNPKVTDTSRSSVSLAWSKPIYDGGAPVKGYWEVKEAAADEWTTCTPPTGLQ
GKQFTVTKLKENTEYNFRICAINSEGVGEP ATLPGSVV AQERIEPPEIELDADLRKWVLRA
SATLRLFVTIKGRPEPEVKWEKAEGILTDRAQIEVTSSFTMLVIDNVTRFDSGRYNLTLENN
SGSKTAFVNVRVLDSPSAPVNLTIREVKKDSVTLSWEPPLIDGGAKITNYIVEKRETTRKAY
ATITNNCTKTTFRIENLQEGCSYYFRVLASNEYGIGLPAETTEPVKVSEPPLPPGRVTLVDVT
RNTATIKWEKPESDGGSKITGYWEMQTKGSEKWSTCTQVKTLEATISGLTAGEEYVFRV
AAVNEKGRSDPRQLGVPVIARDIEIKPSVELPFHTFNVKAREQLKIDVPFKGRPQATVNWR
KDGQTLKETTRVNVSSSKTVTSLSIKEASKEDVGTYELCVSNSAGSITVPITIIVLDRPGPPGP
IRIDEVSCDSITISWNPPEYDGGCQISNYΓVEKKETTSTTWHIVSQAVARTSIKIVRLTTGSEY
QFRVCAENRYGKSSYSESSAVVAEYPFSPPGPPGTPKWHATKSTMLVTWQVPVNDGGSR
VIGYHLEYKERSSILWSKANKILIADTQMKVSGLDEGLMYEYRVYAENIAGIGKCSKSCEP
VPARDPCDPPGQPEVTNITRKSVSLKWSKPHYDGGAKITGYIVERRELPDGRWLKCNYTNI
QETYFEVTELTEDQRYEFRVFARNAADSVSEPSESTGPIΓVKDDVEPPRVMMDVKFRDVIV VBLA.GEVLKINADIAGRPLPVISWAKDGIEIEERARTEIISTDNHTLLTVKDCIRRDTGQYVLT LKNVAGTRSVAVNCKVLDKPGPPAGPLEINGLTAEKCSLSWGRPQEDGGADIDYYΓVEKR ETSHLAWTICEGELQMTSCKVTKLLKGNEYIFRVTGVNKYGVGEPLESVAIKALDPFTVPS
PPTSLEITSVTKESMTLCWSRPESDGGSEISGYIIERREKNSLRWVRVNKKP VYDLRVKSTG
LREGCEYEYRVYAENAAGLSLPSETSPLIRAEDPVFLPSPPSKPKXVDSGKTTITIAWVKPLF
DGGAPITGYTVEYKKSDDTDWKTSIQSLRGTEYTISGLTTGAEYVFRVKSVNKVGASDPSD
SSDPQIAKEREEEPLFDIDSEMRKTLIVKAGASFTMTVPFRGRPVPNVLWSKPDTDLRTRAY
VDTTDSRTSLTIENANRNDSGKYTLTIQNVLSAASLTLWKVLDTPGPPTNITVQDVTKESA
VLSWDVPENDGGAPVKNYHIEKREASKKAWVSVTNNCNRLSYKVTNLQEGAIYYFRVSG
ENEFGVGIPAETKEGVKITEKPSPPEKLGVTSISKDSVSLTWLKPEHDGGSRIVHYVVEALE
KGQKNWVKCAV AKSTHHVVSGLRENSEYFFRVFAENQAGLSDPRELLLPVLIKEQLEPPEI
DMKNFPSHTVYVRAGSNLKVDIPISGKPLPKVTLSRDGVPLKATMRFNTEITAENLTINLKE
SVTADAGRYEITAANSSGTTKAFINIVVLDRPGPPTGPVVISDITEESVTLKWEPPKYDGGSQ
VTNYILLKRETSTAVWTEVSATVARTMMKVMKLTTGEEYQFRIKAENRFGISDHIDSACVT
VKLPYTTPGPPSTPWVTNVTRESITVGWHEPVSNGGSAVVGYHLEMKDRNSILWQKANKL
VIRTTHFKVTTISAGLIYEFRVYAENAAGVGKPSHPSEPVLAIDACEPPRNVRITDISKNSVSL
SWQQPAFDGGSKITGYIVERRDLPDGRWTKASFTNVTETQFIISGLTQNSQYEFRVFARNA
VGSISNP SEVVGPITCIDSYGGPVIDLPLEYTEVVKYRAGTSVKLRAGISGKP APTIE WYKDD
KELQTNALVCVENTTDLASILIKD ADRLNSGCYELKLRNAMGSASATIRVQILDKPGPPGGP
IEFKTVTAEKITLLWRPPADDGGAKITFfYIVEKRETSRVVWSMVSEHLEECIITTTKIIKGNE
YIFRVRAVNKYGIGEPLESDSVVAKNAFVTPGPPGIPEVTKITKNSMTWWSRPIADGGSDI
SGYFLEKRDKKSLGWFKVLKETIRDTRQKVTGLTENSDYQYRVCAVNAAGQGPFSEPSEF
YKAADPIDPPGPPAKIRIADSTKSSITLGWSKPVYDGGSAVTGYWEIRQGEEEEWTTVSTK
GEVRTTEYWSNLKPGVNYYFRVSAVNCAGQGEPIEMNEPVQAKDILEAPEIDLDVALRTS
VIAKAGEDVQVLIPFKGRPPPTVTWRKDEKNLGSDARYSIENTDSSSLLTIPQVTRNDTGKY
ILTIENGVGEPKSSTVSVKVLDTPAACQKLQVKHVSRGTVTLLWDPPLIDGGSPIINYVIEKR
DATKRTWSVVSHKCSSTSFKLIDLSEKTPFFFRVLAENEIGIGEPCETTEPVKAAEVPAPIRD
LSMKDSTKTSVILSWTKPDFDGGSVITEYWERKGKGEQTWSHAGISKTCEIEVSQLKEQS
VLEFRVFAKNEKGLSDPVTIGPITVKELIITPEVDLSDIPGAQVTVRIGHNVHLELPYKGKPK
PSISWLKDGLPLKESEFVRFSKTENKITLSIKNAKKEHGGKYTVILDNAVCRIAVPITVITLGP
PSKPKGPIRFDEIKADSVILSWDVPEDNGGGEITCYSIEKRETSQTN WKMVCSSVARTTFKV
PNLVKDAEYQFRVRAENRYGVSQPLVSSIIVAKHQFRIPGPPGKPVIYNVTSDGMSLTWDA
PVYDGGSEVTGFHVEKKERNSILWQKVNTSPISGREYRATGLVEGLDYQFRVYAENSAGL
SSPSDPSKFTLAVSPVDPPGTPDYIDVTRETITLKWNPPLRDGGSKΓVGYSIEKRQGNERWV
RCNFTDVSECQYTVTGLSPGDRYEFRIIARNAVGTISPPSQSSGIIMTRDENVPPIVEFGPEYF
DGLIIKSGESLRIKALVQGRPVPRVTWFKDGVEIEKRMNMEITDVLGSTSLFVRDATRDHR
GVYTVEAKNASGSAKAEIKVKVQDTPGKWGPIRFTNITGEKMTLWWDAPLNDGCAPITH
YIIEKRETSRLAWALIEDKCEAQSYTAIKLINGNEYQFRVSAVNKFGVGRPLDSDPVVAQIQ
YTVPDAPGIPEPSNITGNSITLTWARPESDGGSEIQQYILERREKKSTRWVKVISKRPISETRF
KVTGLTEGNEYEFHVMAENAAGVGPASGISRLIKCREPVNPPGPPTWKVTDTSKTTVSLE
WSKPVFDGGMEIIGYIIEMCKADLGDWHKVNAEACVKTRYTVTDLQAGEEYKFRVSAING
AGKGDSCEVTGTIKAVDRLTAPELDID ANFKQTHWRAGASIRLFIAYQGRPTPTAVWSKP
DSNLSLRADIHTTDSFSTLTVENCNRNDAGKYTLTVENNSGSKSITFTVKVLDTPGPPGPITF
KDVTRGSATLMWDAPLLDGGAWHHYVVEKREASRRSWQVISEKCTRQIFKVNDLAEGVP
YYFRVSAVNEYGVGEPYEMPEPIVATEQPAPPRRLDWDTSKSSAVLAWLKPDHDGGSRIT
GYLLEMRQKGSDFWVEAGHTKQLTFTVERLVEKTEYEFRVKAKNDAGYSEPREAFSSVII
KEPQIEPTADLTGITNQLITCKAGSPFTIDVPISGRPAPKVTWKLEEMRLKETDRVSITTTKD
RTTLTVKDSMRGDSGRYFLTLENTAGVKTFSVTWVIGRPGPVTGPIEVSSVSAESCVLSW
GEPKDGGGTEITNYΓVEKRESGTTAWQLVNSSVKRTQIKVTHLTKYMEYSFRVSSENRFGV
SKPLESAPIIAEHPFVPPSAPTRPEVYHVSANAMSIRWEEPYHDGGSKIIGYWVEKKERNTIL
WVKENKVPCLECNYKVTGLVEGLEYQFRTY ALNAAGVSKASEASRPIMAQNPVDAPGRP
EVTDVTRSTVSLIWSAPAYDGGSKVVGYIIERKPVSEVGDGRWLKCNYTIVSDNFFTVTAL
SEGDTYEFRVLAKNAAGVISKGSESTGPVTCRDEYAPPKAELDARLHGDLVTIRAGSDLVL DAAVGGKPEPKIIWTKGDKELDLCEKVSLQYTGKRATAVIKFCDRSDSGKYTLTVKNASG
TKAVSVMVKVLDSPGPCGKLTVSRVTQEKCTLAWSLPQEDGGAEITHYIVERRETSRLNW
VIVEGECPTLSYWTRLIKNNEYIFRVRAVNKYGPGVPVESEPTVARNSFTIPSPPGIPEEVGT
GKEHIIIQWTKPESDGGNEISNYLVDKREKKSLRWTRVNKDYVVYDTRLKVTSLMEGCDY
QFRVTAVNAAGNSEPSEASNFISCREPSYTPGPPSAPRWDTTKHSISLAWTKPMYDGGTDI
VGYVLEMQEKDTDQWYRVHTNATIRNTEFTVPDLKMGQKYSFRVAAVNVKGMSEYSESI
AEIEPVERIEIPDLELADDLKKTVTIRAGASLRLMVSVSGRPPPVITWSKQGIDLASRAIIDTT
ESYSLLIVDKVNRYDAGKYTIEAENQSGKKSATVLVKVYDTPGPCPSVKVKEVSRDSVTIT
WEIPTIDGGAPVNNYΓVEKREAAMRAFKTVTTKCSKTLYRISGLVEGTMYYFRVLPENΓYGI
GEPCETSDAVLVSEVPLVP AKLEWDVTKSTVTLAWEKPLYDGGSRLTGYVLEACKAGTE
RWMKWTLKPTVLEHTVTSLNEGEQYLFRIRAQNEKGVSEPRETVTAVTVQDLRVLPTIDL
STMPQKTIHVPAGRPVELVIPIAGRPPPAASWFFAGSKLRESERVTVETHTKVAKLTIRETTI
RDTGEYTLELKNVTGTTSETIKVIILDKPGPPTGPIKIDEIDATSITISWEPPELDGGAPLSGYV
VEQRDAHRPGWLPVSESVTRSTFKFTRLTEGNEYVFRVAATNRFGIGSYLQSEVIECRSSIRI
PGPPETLQIFDVSRDGMTLTWYPPEDDGGSQVTGYIVERKEVRADRWVRVNKVPVTMTR
YRSTGLTEGLEYEHRVTAINARGSGKPSRPSKPΓVAMDPIAPPGKPQNPRVTDTTRTSVSLA
WSVPEDEGGSKVTGYLIEMQKVDQHEWTKCNTTPTKIREYTLTHLPQGAEYRFRVLACNA
GGPGEPAEVPGTVKVTEMLEYPDYELDERYQEGIFVRQGGVIRLTIPIKGKPFPICKWTKEG
QDISKRAMIATSETHTELVIKEADRGDSGTYDLVLENKCGKKAVYIKVRVIGSPNSPEGPLE
YDDIQVRSVRVSWRPPADDGGADILGYILERREVPKAAWYTIDSRVRGTSLWKGLKENV
EYHFRVSAENQFGISKPLKSEEPVTPKTPLNPPEPPSNPPEVLDVTKSSVSLSWSRPKDDGGS
RVTGYYIERKETSTDKWVRHNKTQITTTMYTVTGLVPDAEYQFRIIAQNDVGLSETSPASE
PWCKDPFDKPS.QPGELEILSISKDSVTLQWEKPECDGGKEILGYWVEYRQSGDSAWKKSN
KERIKDKQFTIGGLLEATEYEFRVFAENETGLSRPRRTAMSIKTKLTSGEAPGIRKEMKDVT
TKLGEAAQLSCQΓVGRPLPDIKWYRFGKELIQSRKYKMSSDGRTHTLTVMTEEQEDEGVYT
CIATNEVGEVETSSKLLLQATPQFHPGYPLKEKYYGAVGSTLRLHVMYIGRPVPAMTWFH
GQKLLQNSENITIENTEHYTHLVMKNVQRKTHAGKYKVQLSNVFGTVDAILDVEIQDKPD
KPTGPIVIEALLKNSAVISWKPPADDGGSWITNYWEKCEAKEGAEWQLVSSAISVTTCR[V
NLTENAGYYFRVSAQNTFGISDPLEVSSVVIIKSPFEKPGAPGKPTITAVTKDSCVVAWKPP
ASDGGAKIRNYYLEKREKKQNKWISVTTEEIRETVFSVKNLIEGLEYEFRVKCENLGGESE
WSEISEPITPKSDVPIQAPHFKEELRNLNVRYQSNATLVCKVTGHPKPIVKWYRQGKEIIAD
GLKYRIQEFKGGYHQLIIASVTDDDATVYQVRATNQGGSVSGTASLEVEVPAKIHLPKTLE
GMGAVHALRGEWSIKIPFSGKPDPVITWQKGQDLIDNNGHYQVIVTRSFTSLVFPNGVER
KDAGFYVVCAKNRFGIDQKTVELDVADVPDPPRGVKVSDVSRDSVNLTWTEPASDGGSKI
TNYIVEKCATTAERWLRVGQARETRYTVINLFGKTSYQFRVIAENKFGLSKPSEPSEPTITK
EDKTRAMNYDEEVDETREVSMTKASHSSTKELYEKYMIAEDLGRGEFGIVHRCVETSSKK
TYMAKFVKVKGTDQVLVKKEISILNIARHRNILHLHESFESMEELVMIFEFISGLDIFERΓNTS
AFELNEREIVSYVHQVCEALQFLHSHNIGHFDIRPENHYQTRRSSTIKIIEFGQARQLKPGDN
FRLLFTAPEYYAPEVHQHDVVSTATDMWSLGTLVYVLLSGINPFLAETNQQIIENIMNAEY
TFDEEAFKEISIEAMDFVDRLLVKERKSRMTASEALQHPWLKQKIERVSTKVIRTLKHRRY
YHTLIKKDLNMVVSAARISCGGAIRSQKGVSVAKVKVASIEIGPVSGQIMHAVGEEGGHVK
YVCKIENYDQSTQVTWYFGVRQLENSEKYEITYEDGVAILYVKDITKLDDGTYRCKWND
YGEDSSYAELFVKGVREVYDYYCRRTMKKIKRRTDTMRLLERPPEFTLPLYNKTAYVGEN
VRFGVTITVHPEPHVTWYKSGQKIKPGDNDKKYTFESDKGLYQLTINSVTTDDDAEYTVV
ARNKYGEDSCKAKLTVTLHPPPTDSTLRPMFKRLLANAECQEGQSVCFEIRVSGIPPPTLK
WEKDGQPLSLGPNIEIIHEGLDYYALHIRDTLPEDTGYYRVTATNTAGSTSCQAHLQVERL
RYKKQEFKSKEEHERHVQKQIDKTLRMAEILSGTESVPLTQVAKEALREAAVLYKPAVST
KTVKGEFRLEIEEKKEERKLRMPYDVPEPRKYKQTTIEEDQRIKQFVPMSDMKWYKKIRD
QYEMPGKLDRWQKRPKRIRLSRWΈQFYVMPLPRITDQYRPKWRIPKLSQDDLEΓVRPARR
RTPSPDYDFYYRPRRRSLGDISDEELLLPIDDYLAMKRTEEERLRLEEELELGFSASPPSRSPP
HFELSSLRYSSPQAHVKVEETRKDFRYSTYHIPTKAEASTSYAELRERHAQAAYRQPKQRQ
RIMAEREDEELLRPVTTTQHLSEYKSELDFMSKEEKSRKKSRRQREVTEITEIEEEYEISKHA
QRESSSSASRLLRRRRSLSPTYIELMRPVSELIRSRPQPAEEYEDDTERRSPTPERTRPRSPSP VSSERSLSRFERSARFDIFSRYESMKAALKTQKTSERKYEVLSQQPFTLDHAPRITLRMRSH
RVPCGQNTRFILNVQSKPTAEVKWYHNGVELQESSKIHYTNTSGVLTLEILDCHTDDSGTY
RAVCTNYKGEASDYATLDVTGGDYTTYASQRRDEEVPRSVFPELTRTEAYAVSSFKKTSE
MEASSSVREVKSQMTETRESLSSYEHSASAEMKSAALEEKSLEEKSTTRKIKTTLAARILTK
PRSMTVYEGESARFSCDTDGEPVPTVTWLRKGQVLSTSARHQVTTTKYKSTFEISSVQASD
EGNYSVWENSEGKQEAEFTLTIQKARVTEKAVTSPPRVKSPEPRVKSPEAVKSPKRVKSPE
PSHPKAVSPTETKPTPTEKVQHLPVSAPPKITQFLKAEASKEIAKLTCWESSVLRAKEVTW
YKDGKKLKENGHFQFHYSADGTYELKINNLTESDQGEYVCEISGEGGTSKTNLQFMGQAF
KSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVΓVTGLQDTTVSSDSVAKFAVK
ATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSS
SCKLTIKAIKDTEAQKVSTQKTSEITPQKKAWQEEISQKALRSEEIKMSEAKSQEKLALKE
EASKVLISEEVKKSAATSLEKSΓVΉEEITKTSQASEEVRTHAEIKAFSTQMSINEGQRLVLKA
NIAGATDVKWVLNGVELTNSEEYRYGVSGSDQTLTIKQASHRDEGILTCISKTKEGΓ/KCQ
YDLTLSKELSDAP AFISQPRSQNINEGQNVLFTCEISGEPSPEIE WFKNNLPISISSNVSISRSR
NVYSLEIRNASVSDSGKYTIKAKNFRGQCSATASLMVLPLVEEPSREWLRTSGDTSLQGSF
SSQSVQMSASKQEASFSSFSSSS-ASSMTEMKFASMSAQSMSSMQESFVEMSSSSFMGISNM
TQLESSTSKMLKAGIRGIPPKIEALPSDISIDEGKVLTVACAFTGEPTPEVTWSCGGRKIHSQE
QGRFHIENTDDLTTLIIMDVQKQDGGLYTLSLGNEFGSDSATVNIHIRSI
Additional isoforms exist; namely, isoform N2B (NP_003310); isofoπn novex-1 (NP_597676); isoform novex-2 (NP_597681); isoform novex-3 (NP_596870).
[0398] Following is a LOCI 12609 amino acid sequence (SEQ ID NO: 24). NP_612418
MSAQRLISNRTSQQSASNSDYTWEYEYYEIGPVSFEGLKAHKYSRVIGFWVGLAVFVIFMFI VLTLLTKTGAPHQDNAESSEKRFRMNSFVSDFGRPLEPDKVFSRQGNEESRSLFHCYINEVE RLDRAKACHQTTALDSDVQLQEAIRSSGQPEEELNRLMKFDIPNFVNTDQNYFGEDDLLIS EPPΓVLETKPLSQTSHKDLD
[0399] Following is a TRPSl amino acid sequence (SEQ ID NO: 25). NP_054831
MVRKKNPPLRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELN
HKEEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNML
AFSFP AAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQ
AQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLVNDNPDPAPLSPELQD
FKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHS
KI)FQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKY
FRCKFCNFTYMGNSSTELEQHFLQTHPNKIKASLPSSEVAKPSEKNSNKSIP ALQSS
DSGDLGKWQDKITVKAGDDTPVGYSVPIKPLDSSRQNGTEATSYYWCKFCSFSCE
SSSSLKLLEHYGKQHGAVQSGGLNPELNDKLSRGSVINQNDLAKSSEGETMTKTD
KSSSGAKKKDFSSKGAEDNMVTSYNCQFCDFRYSKSHGPDVIVVGPLLRHYQQLH
NIHKCTIKHCPFCPRGLCSPEKHLGEITYPFACRKSNCSHCALLLLHLSPGAAGSSR
VKHQCHQCSFTTPDVDVLLFHYESVHESQASDVKQEANHLQGSDGQQSVKESKE
HSCTKCDFITQVEEEISRHYRRAHSCYKCRQCSFTAADTQSLLEHFNTVHCQEQDIT
TANGEEDGHAISTIKEEPKIDFRVYNLLTPDSKMGEPVSESVVKREKLEEKDGLKE
KVWTESSSDDLRNVTWRGADILRGSPSYTQASLGLLTPVSGTQEQTKTLRDSPNVE
AAHLARPIYGLAVETKGFLQGAPAGGEKSGALPQQYPASGENKSKDESQSLLRRR
RGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQII
RRRTRKRLNPEALQAEQLNKQQRGSNEEQVNGSPLERRSEDHLTESHQREIPLPSLS KYEAQGSLTKSHSAQQPVLVSQTLDIHKRMQPLHIQIKSPQESTGDPGNSSSVSEGK
GSSERGSPIEKYMRPAKHPNYSPPGSPIEKYQYPLFGLPFVHNDFQSEADWLRFWS
KYKLSVPGNPHYLSHVPGLPNPCQNYVPYPTFNLPPHFSAVGSDNDIPLDLAIKHSR
PGPTANGASKEKTKAPPNVKNEGPLNVVKTEKVDRSTQDELSTKCVHCGIVFLDE
VMYALHMSCHGDSGPFQCSICQHLCTDKYDFTTHIQRGLHRNNAQVEKNGKPKE
[0400] Following is a PRAME amino acid sequence (SEQ ED NO: 26). NP_006106
MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLF
MAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQ
EVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGL
STEAEQPFIP VEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIF AMPM
QDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISP
EKEEQYIAQFTSQFLSLQCLQAL YVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLS
EGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGIT
DDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYE
DIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCF
MPN
[0401] Following is a ZNF221 amino acid sequence (SEQ ID NO: 27). NP_037491
MISPSLELLHSGLYKFPEVEGKMTTFKEAVTFKDVAWFTEEELGLLDPAQRKLYRDVMLE
NFRNLLSVGNQPFHQDTFHFLGKEKFWKMKTTSQREGNSGGKIQIEMETVPEAGPHEEWS
CQQIWEQIASDLTRSQNSIRNSSQFFKEGDVPCQIEARLSISHVQQKPYRCNECKQSISDVSV
FDLHQQSHSGEKSHTCGECGKSFCYSPALHIHQRVHMGEKCYKCDVCGKEFNQSSHLQTH
QRVHTGEKPFKRGQCGKGFHSRSALNVHCKLHTGEKPYNCEECGKAFIHDSQLQEHQRIH
TGEKPFKCDICGKSFRVRSRLNRHSMVHTGEKPFRCDTCGKNFRQRS ALNSHSMVHIEEKP
YKCEQCGKGFICRRDFCKHQMVHTGEKPYNCKECGKTFRWSSCLLNHQQVHSGQKSFKC
EECGKGFYTNSRRSSHQRS-HNGEKPYNCEECGKDYKRRLDLEFHQRVHTGERPYNCKECG
KSFGWASCLLKHQRLHSGEKPFKCEECGKRFTQSTQLHSHQTCHTGEKLYKCEQCEKGYN
SKFNLDMHQRVHRGERPYNCKECGKSFGWASCLLKHQRLHSGEKPLKSGVWEEIYSEFTA
SFTSVSLCGRKAI
[0402] Following is a FOXA2 amino acid sequence (SEQ ID NO: 28).
MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGN
MSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGG
QAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQS
PNKMLTLSEΓYQWIMDLFPFYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGKGSFWTLH
PDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGAQASQAQLGEAAGPA
SETP AGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQAAAHLLGPPHHP
GLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHH[Q]PHKMDLKAYEQVMHYPGY GSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
Synonymous coding change in brackets [0403] Following is a JAMS amino acid sequence (SEQ ID NO: 29). NP_116190 -junctional adhesion molecule 3 precursor [Homo sapiens].
MVPARLGPAVAMVTGAGRRVLAGWAHARGDYKPRRAAAGPSATLDMALRRPPRLRLCA
RLPDFFLLLLFRGCLiGA VNLKSSNRTPWQEFESVELSCΠTDSQTSDPPJE WKKIQDEQTTY VFFDNKIQGDLAGRAEILGKTSLKIWNVTRRDSALYRCEVVARNDRKEIDEΓVIELTVQVKP VTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNS ETGTLVFTA VHKDDSGQYYCIASNDAGSARCEEQEMEVYDLNIGGIIGGVLVVLAVLALIT LGICCAYRRGYFINNKQDGESYKNPGKPDGVNYIRTDEEGDFRHKSSFVI
[0404] Following is a GLB amino acid sequence (SEQ ID NO: 30).
NP_000159 - GLI-Kruppel family member GLB; oncogene GLB; DNA-binding protein; zinc finger protein GLB [Homo sapiens].
MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRN
AITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDP
RNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPD
LPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHA
GVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSA
RPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPA
LSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQ
VSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLV
KEEGDKDESKQEPEVIYETNCHWEGC AREFDTQEQLVHHINNDHIHGEKKEFVCR WLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSH TGEKP YVCEHEGCNKAFSNASDRAKHQNRTHSNEKP YVCKIPGCTKRYTDPSSLR
KHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSN
TTSKREECLQ VKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSI
GDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRL
NPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDS
SASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSE
ASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDA
LEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDALGHGVRRASDPVRTGSEGLAL
PRVPRFSSLSSCNPP AMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLE
SLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFD
APGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVP
QTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSG
NAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNF
GLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQI
SATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLAL
QSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGS
FSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSS
LMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKF
LAVMQ
[0405] Modifications may be made to the foregoing without departing from the basic aspects of the invention. Although the invention has been described in substantial detail with reference to one or more specific embodiments, those of skill in the art will recognize that changes may be made to the embodiments specifically disclosed in this application, yet these modifications and improvements are within the scope and spirit of the invention, as set forth in the claims which follow. Also, citation of the above publications or documents is not intended as an admission that any of the foregoing is pertinent prior art, nor does it constitute any admission as to the contents or date of these publications or documents. Each patent, patent application and other publication and document referenced are incorporated herein by reference in its entirety, including drawings, tables and cited documents.

Claims

What is claimed is:
1. A method for identifying a subject at risk of type II diabetes, which comprises detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in a nucleic acid sample from a subject, wherein the one or more polymorphic variations are detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; whereby the presence of the polymorphic variation is indicative of the subject being at risk of type II diabetes.
2. The method of claim 1, which further comprises obtaining the nucleic acid sample from the subject.
3. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rsl 888727, rs998203, rs600324, rs2297366, rs2297367, rs755883, rs755884, rs2297369, rs2297370, rs2297371, rs2297372, rslO5OO15, rsl479355, rs457543, rs456598, rs2297373, rsl867351, rs461473, rs2777353, rs435593, rs3822841, rs683369, rs3798175, rs3798174, rs3737088, rs697735, rs3777394, rs3822840, rs366049, rs366048, rs594709, rs3798173, rs3798172, rs806383, rs3798170, rs3798169, rs3777393, rs3777392, rs2282142, rs2282143, rsl871388, rs3798168, rs628031, rsl867350, rs662138, rs644992, rs637841, rs3798167, rs2197296, rs619598, rs622342, rs715083, rsl382785, rs650284, rsl443844, rs3798164, rs2297374, rs3798163, rs3798162, rs3818678, rs609468, rsl804945, rs651164, rs2083867, rs3101826, rs2343403, rsl871389, rsl479353, rs959814, rs719631, rs791194, ts705771, rs791195, rs791196, rs3911977, rs791198, rs791174, rs791175, rs791176, «3861971, rs3894314, rs791179, rs791183, rs791184, rs791185, rs3103346, rs791186, rs791187, rs791188, rs791189 and rs791190.
4. The method of claim 1, wherein the one or more polymorphic variations, are detected at one or more positions selected from the group consisting of rs456598, rs3798168, rs662138, rs644992, rs637841, rs622342, rs650284 and rs2297374.
5. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 24,609 to 61,673 in SEQ ID NO: 1.
6. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2195070, rs2303537, rs967507, rsl063160, rsl047500, rs2366577, rs2288324, rs956514, rs957875, rs3217295, rs2857273, rs2857272, rs2857271, rs3829749, rs3813250, rs3813249, rs2857269, rs3829748, rs2857268, rs3829747, rs3731753, rs2857266, rs3731752, rs2857265, rs962659, rs2278196, rs2857264, rs23035383 rs2303539, rs2288325, rs3820978, rs3731751, rs3731750, rs3731749, rs2555811, rs2555812, rs2555813, rs2555814, rs2555815, rs2857267, rs2555816, rs2555817, rs2288326, rs2555818, ^2555819, rs2288327, rs3731748, rs747122, rs890578, rs3815874, rs3731747, rs2366751, rs3829746, rs3813247, rs3731746, rs3731745, rs3731744, rs3813246, rs3813245, rs3813244, rs3813243, rs3731743, rs744428, rs744426, rs2288572, rs3769864, rs2288571, rs2288570, rs2303838, rs2303837, rs2042996, rsl007025, rsl560221, rs2163009, rs2303836, rs2288569, rs2303835, rs3214578, rs2288568, τsl001238, ra2303834, rs2303833, rs2303832, rs2303831, rs2288567, rs2288566, rs2115559, rs2115558, rs3214577, rs2288565, rs2303830, rsl429095, rs3835071, rsl864243, rs2303829, rs2366752, rslO17323, rs2288563, rs2163010, rs2742324, rs2562843, rsl569407, rs2243452, rs3731741, rs2246932, rs2562844, rs3754951, rs!469473, rsl366677, rs2562845, rs2742356, rs2742355, rs2562846, rsl429094, fs2742354, rs2163008, rs2115557, rs2562848, rs2562849, rs2742353, rs2562&50, rs2742352, rs2252353, rs2252350, rs2472751, rs2742351, rs2742350, rs2742349, rs2303828, rs2288564, rs2627038, rs2244492, rs2042995, rs3045696 and rs2217347.
7. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2195070, rs967507, rs2366577, rs3829747, rs2857265, rs2303539, rs3731750, rs3731749, rs747122, rs2366751, rs3813247, rs3731745, rs3813246, rs3813245, rs744426, rs2042996, rsl560221, rs2288569, rs2288568, rsl001238, rs2288566, rs2288563, rs2243452, rs2246932, rs2562844, rsl366677, rs2742355, rs2562846, rs2163008, rs2562848, rs2562849, rs2742353, rs2252353, rs2472751, rs2742350, rs2742349, rs2627038 and rs2042995.
8. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 244 to 193,209 in SEQ ID NO: 2.
9. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs6936939, rs6941487, rs6941805, rs6941892, rs6899315, rs6942240, rsl928279, rs2497106, rs7754453, rs2497105, rs7775786, rs2480185, rs2480186, rs2497151, rs2480187, rs2480188, rs2480189, rs2497160, rs2497162, rs2497108, rs6937611, rs2497113, rs2497115, rs2480191, rs2497116, rs2497117, rs2497118, rs7776208, rs7756256, rs7756316, rs7756587, rs2480192, rs2497119, rs6906099, rs6926656, rsl928281, rs2225244, rs7773403, rsl556111, rs2497120, rs6913756, rs6918019, rs2497146, rs2497145, rsl928282, rs2480194, rs2480195, rs6936847, rs6914226, rs7383205, rs6933393, rs6922368, rs2480196, rs6928025, rs6928551, rs2497136, rs6928815, rsl810806, rs2497135, rs6934877, rs2497133, rs2497132, rs2497131, rs2480197, rs2875382, rs7450001, rs2497130, rs2497129, rslO45649, rs3219, rs8348, rs3218, rs2497127, rs2497126, rs2480198, rs2480199, rs2497123, rs2497122, rs6902104, rs2497121, rs2480200, rs2480201, rs.1928283, rs9449785, rs5877871, rsl080960, rsl 114811, rs4097471, rs4097472, rs3857473, rs2476913, rs2476894, rs2324637, rs9449794, rs7741955, rs2476912, rs6901546, rs3778180, rs6913525, rs2497148, rs6930183, rs6936765, rs7771595, rs7775669, rs2497149, rs2497150, ra728721, rs2476899, and rs2476900.
10. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2480187, rs6926656, rs2497120, rs2480195, rs6914226, rs6922368, rs2480196, rs2497136, rs2497131, rs2480197, •rs3219, rs2497126, rs2480201, rs2476913, rs9449794, rs7741955, and rs2497150.
11. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 10416 to 96459 in SEQ ID NO: 3.
12. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2625681, rs2625680-, rs2625678, rs2625676, rs2737202, rsl905376, rs2178946, rs800919, rs2142327, rs2884365, rs800918, rs800917, rs800916, rs2625683, rs2625682, rsl483583, rs38O8432, rsl483584, rsl483585, rs800912, rs3779878, rs3808433, rs3808434, rs3808435, rs3808436, rs3808437, rs3824201, rs3808438, rs3808439, rs3808440, rsl351744, rsl483589, rs3802219, rs2884366, rs4633093, rs2358019, rsl872075, rs3824202, rs3824203, rs3808444, rs3802221, rs3779879, rs3779880, rs3808447, rs38O8448, rs3808449, rs3808450, rs3808451, rs2223103, rsl 160333, rs3808452, rslO28274, rsl963677, rsl963678, rs3779881, rs2358020, rs2358021, rs2049864, rs2049866, rs3808456, rs4876606, rs4876607, rs3808457, rs4876343, rs4876608, rs3779882, rs719422, rs2178949, rs2272619, rslO57517, rs3808460, rs3808461, rs4141424, rs2293889, rs3802222, rs2737203, rs2721954, rs2721968, rs2721969, rs3808462, rs2049869, rs2721927, rs2737205, rs2721928, rs2721929, rs3779883, rs4876609, rs2737207, rs2737208, rs2737210, rs2737211, rs2721930, rs2244644, rs2721932, rs2245019, rs2976473, rs2737213, rs2245221, rs2737214, rs2721933, rs2721935, rs2737216, and rs2737217.
13. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rsl905376, rs2178946, rsl483583, rs3779878, rs3808435, rs3808437, rs3808438, rs3808440, rsl483589, rs3802219, rs2884366, rs3779879, rs3808447, rs3808448, rs3808450, rs2223103, rsl 160333, rslO28274, rsl963677, rs3779881, rs2358021, rs2049864, rs2049866, rs3808456, rs3808457, rs719422, rs2178949, rs2272619, rs3808461, rs2737203, rs2737205, rs2721929, rs2737207, rs2721932, rs2245019, rs2245221, rs2737214, and rs2737216.
14. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 2040 to 98361 in SEQ ID NO: 4.
15. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rsl 1109, rs361737, AA at position 522, rs2236729, rs2236730, rs2051486, rs2051487, rs2051488, rs965764, rs2006783, rs2032416, rs362139, AB at position 19890, rs361901, rs361676, rs362184, rs.361797, rs361907, rs362004, rs362176, rs361570, rs361595, rs361778, rs361709, rs361558, rs361762, rs361737, rs362003, rs362011, rs361721, rs361959, rs362230, rs361970, rs362012, rs361671, rs361876, rs362168, rs361830, rs362257, rs362167, rs.362O15, rs361976, rs362044, rs362245, rs361986, rs362241, rs361582, rs362208, rs361755, rs361864, rs362107, rs361535, rs361940, rs361828, rsl 1090131, rs361894, rs361829, rs2156888, rs2156889, rs2156890, rs738887, rs738888, rs3827305, rs3747098, rs8405, rs7104, rsl3604, rs2229696, rs2072049, rslO24372, rslO24373, rs2073725, rs2072050, rs2229695, rs739463, rs3216404, rs2266981, rs2266982, rs2266983, rs2266984, rs2266985, rs2266988, rs2283798, rs2283799, rs2283800, rs2073916, rs2330188, rs2877079, rs2330189, rs424233, rs424248, rs2330190, rs2266991, rs2266992, rs3827306, rs3827307, rs2859957, rs737891, rs2097206, rs363968, rs2078843, rs2330191, rs2330192, rs2877080, rs715594, rs715591, rs715587, rs715593, rs459197, rs467133, rs723182, rs460606, rs457907, rs460608, rs457967, rsl807539, rs464317, rs459939, rs466341, rs2156893, rs9306380, rs2156895, rs2187589, rs429560, rs2236731, rs2236732, rs392546, rs2236733, rsl557617, rsl557618, rs715516, rs741673 and rs2330193.
16. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rsll 109, rs361709, rs362012, rs362167, rs361976, rs361864, rs362107, rs361940, rs361828, rs361829, rs2156888, rs2156889, rs3827305, rs7104, rsl3604, rslO24372, rs2283799, rs2877079, rs2266991, rs3827306, rs460608, rs457967, rs2236732 and rs715516.
17. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 210 to 94819 in SEQ ID NO: 5.
18. The method of claim 1 , wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs374652, rs450308, rs424729, rs423752, rs375066, rs384329, rs424410, rs396063, rs398388, rs404547, rs413093, rs379785, rs428505, rs454559, rs367741, rs444350, rs373168, rs368316, rs411803, rs419990, rs445865, rs386747, rs386729, rs454376, rs453682, rs452143, rs444816, rs377931, rs365769, rs379722, rs2136302, rs396188, rs424041, rs386249> rs385202, rs394380, rs388547, rs442518, rs2356537, rs6509123, rs2356538, rs424673, rs407375, rs431230, rsl703747, rs390134, rs415468, rs367241, rs2284243, rs414122, rs446072, rs47775, rs373043, rs438174, rs429611, rs714420, rs442543, rs2356543, rs391596, rs441386, rs407806, rs381872, rs393473, rs393403, rs393117, rs413061, rs383133, rs397276, rs369958, rs377174, rs451601, rs451612, rs453932, rs439676, rs453640, rs365745, rs366111, rs376769, rs447175, rs457642, rs2535490, rs461374, rs465860, rs385016, rs364378, rs2191565, rs382715, rs397133, rs397706, rs390784, rs398981, rs425530, rs415499, rs415521, rs384596, rs431166, rs436790, rs392889, rs391267, rs414402, rs381888, rs393195, rs405763, rs370809, rs397964, rs397683, rs382255, rs381983, rs396497, rs396148, rs396116, rs395803, rs448692, rs381759, rs420584, rs381091, rs420355, rs436739, rs419839, rs431509, rs.421505, rs403296, rs393138, rs415913, rs2302411, rs446016, rs434585, rs452554, rs449664, rs367499, rs384544, rs369083, rs380726, rs379238, rs367209, rs2270196, rs369106, rs390369, rs451388, rs430107, rs2356549, rs2191566, rs454904, rs427608, rslO6O877, rsl060880, and rs 12753.
19. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs374652, rs445865, rs2356537, rs2356538, rs2284243, rs442543, rs397276, rs365745, rs366111, rs2191565, rs3818885 rs415913, rs451388, rs430107 and rs2191566.
20. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 209 to 89929 in SEQ ID NO: 6.
21. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs 1415817, rs952991, rs2404166, rs2896772, rs2404167, rsl011104, rsl572580, rsl999861, rsl999862, rsl999863, rsl572581, rsl890206, rsl890207, rsl890208, rsl415818, rs952690, rsl890210, rsl933206, rs2021680, rs2021681, rsl203866, rsl203867, rsl203868, rsl203869, rs945982, rsl203870, rsl203871, rsl203872, rsl203873, rsl203874, rsl203876, rsl203877, rsl203879, rsl203880, rsl203881, rslO44293, rsl203883, rsl203884, rsl203886, rs2471, rsl203888, rsl203889, rsl337925, rsl337926, rsl203890, rsl203891, rs2277762, rs2277763, rsl203892, rs932560, rsl203S93, rsl203894, rsl203895, rsl203896, rsl203897, rsl203898, rsl203899, rsl203901, rsl203903, rsl203904, rsl203905, rsl203906, rsl203907, rsl203908, rsl203909, rsl 1482531, rsl055080, rsl212275, rsl203911, rs2277764, rsl203912, rsl337918, rsl209523, rsl337919, rs2145062, rsl040754, rsl203913, rsl337920, rsl337921, rsl203914, rsl203916, rsl203917, rs910958, rs9109S9, rs2003076, rsl203919, rsl203920, rsl203921, rsl203922, rsl203923, rsl203924, rsl007458, rs995734, rsl203925, rsl 007202, rsl203928, rsl203929, rsl203930, rsl203931, rsl203932, and rsl203933.
22. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs2404167, rs2021681, rsl203868> rs945982, rsl203871, rsl203873, rsl203876, rsl203877, rsl203879, rsl203880, rsl203881, rsl203883, rsl203884, r&1203886, rs2471, rsl203889, rs2277763, rsl203892, rs932560, rsl203894, rsl203896, rsl203898, rsl203899, rsl203905, rsl203907, rsl 1482531, rsl0550805 rs2277764, rsl337918, rsl209523, rsl337919, rsl33792α, rsl337921, rsl203916, rsl203917, rs910958, rs910959, rsl203919 and rs995734.
23. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 17033 to 95608 in SEQ ID NO: 7.
24. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs470855, rs470780, rs674487, rs5795871, rs2510345, rsl784500, rs484233, rs470755, rs470796, rs643141, rs631156, rs630723, rs712S310, rs470982, rs599808, rs470437, rs470434, rs470372, rs470474, rs678161, rs470536, rs676606, rs470461, rs7123926, rs470522, rs470388, rsl892895, rsl 143810, rsl 143812, rs470639, rs470854, rs470565, rs596004, rs596445, rsl271678, rs470960, rs470953, rs639165, rs626717, rs658544, rs621953, rs503044, rs470882, rs470966, rs470425, rs470957, rs610684, rs623823, rs7121086, rs7942767, rs625740, rs624504, rs470829, rs7933094, rs79465805 rs470631, rs470547, rs618700, rs470500, rs595196, rs470846, rsl051036, rs7101690, rs470952, rs4077351, rs4937867, rs470719, rs470713, rs470407, rs470659, rs470570, rs2155088, rs470452, rs470564, rsl 144241, rsl939124, rs470680, rs4264164, rsl783814, rsl271129, rs7947580, rs470936, rs470459, rs470600, rs6590734, rs470608, rs2276266, rs4937868, rs646998, rs560925, rs470809, rs532421, rsl815858, rs627592, rs646513, rs694186, rs595584, rs610382, rs610829, rs79456223 rs597320, rs7936421, rs3839957, rs641024, rs3824994, rs643447, rs655627, rs6590735, rs866908, rs94499, and rs2014708.
25. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs470780, rs2510345, rsl784500, rs470372, rs470474, rs470536, rs470522, rs470639, rs470565, rs470953, rs639165, rs626717, rs658544, rs470882, rs470425, rs610684, rs625740, rs624504, rs470547, rs470500, rs470713, rs470570, rs470936, rs470600, rs470809, rs595584, rs610382, rs597320, and rs2014708.
26. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 230 to 63711 in SEQ ID NO: 8.
27. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions selected from the group consisting of rs846323, rs846322, rs846321, rs846320, rs7811555, rs699490, rs846319, rs846318, rs846317, rs846316, rs699491, rs699492, rs699493, rs2840182, rs4724089, rs4724090, rs6953964, rs846269, rs846268, rsl405753, rsl405752, rs2330282, rsl405751, rs846267, rs846266, rs3898405, rs7806570, rs846265, rs3801162, rs846264, rs7785287, rs846263, rs699494, rs2282920, rs7794103, rs4720415, rs4724091, rs2330284, rs2330285, rsl527500, rs917230, rs917229, rs2237423, rs2237422, rs2237421, rs2237420, rsl527499, rs2237419, rs3030352, rsl527498, rs3801164, rs3801165, rs2282919, rsl527496, rs6960238, rs6960725, rs7798461, rs7798596, rs6965985, rs7799496, rs7799683, rs7782596, rs6966658, rs7799542, rs7799584, rs6971570, rs6971751, rs6959829, rs2237417, rs6960695, rs7776923, rs6960887, rs7797875, rs6965961, rs6966146, rs7802706, rs7786060, rs7803421, rs7803428, rs7786698, rs7804280, rs7807699, rs4321900, rs2237416, rs7787368, rs4430004, rs6954947, rs2237415, rs6463087, rs6959294, rs7793034, rs2074741, rsl405750, rs2072201, rs4724092, rs4724093, rs4724094, rs6968300, rs3801166, rs3801167, rs3801168, rslO24552, rslO12136, rslO12135, rs865860, rs866417, rs846298, rs846297, rs846296, rs846295, rs846294, rs846293, rs846291, rs3801170, rs846290, rs2108166, rs3779144, rs846315, rs846314, rs846313, rs846312, rs846311, rs846310, rs846309, rs846308, rs846307, rs846306, rs846305, rs2108165, rs846282, rs3779145, rs3801174, rs3779146, rs3779147, rs846280, rs3801176, rs699495, rs3823723, rs2330409, rs2877136, rs2877137, rs3801177, rsl949974, rs7787414, rs4576336, rs3839731, rs3801178, rsl527501, rs2141174, rs2141175, rs2178029, rs3779149, rs3057241, rs3801180, rsl918380, rsl918381, rs3801181, rs2141176, rs2330412, rs3801182, rs3839733, rsl534316, rs3801183, rs6463088, rs7802174, rs6463089, rs6463090, rs6463091, rs3801184, rs3801185, rs7800024, rs7785042, rs3801186, rs3801187, rs3779152, rs846400, rs3801189, rs846399, rs846398, rs846397, rs846396, rs846395, • rs3801192, rs699496, rs699498, rs699499, «5883813, rs699500, rs3938299, rs3801194, rs846394, rs3801196, rsl405754, rs699502, rs846393, rs3801198, rs846392, rs846391, rs846390, rs846389, rs846388, rs846387, rs846386, rs846385, rs846382, rsl405755, rs846381, rs846380, rs846378, rs6974655, rs846377, rs2049622, rs720790, rs720791, rs720789, and rs5883814.
28. The method of claim 1, wherein the one br more polymorphic variations are detected at one or more positions selected from the group consisting of rs699493, rs846265, rs7785287, rs846263, rs2282920, rs2330284, rs917229, rs2237422, rs2237420, rsl527499, rs6959829, rs223741S, rs6959294, rs7793034, rsl405750, rs4724092, rs4724093, rs3801168, rslO245.52, rs846291, rs846290, rs846315, rs846312, rs846311, rs846310, rs846309, rs846308, rs846306, rs2108165, rs3801174, rs3801177, rs3839733, rs3801183, rs6463089, rs3801186 and rs3801192.
29. The method of claim 1, wherein the one or more polymorphic variations are detected in a region spanning positions 6109 to 82807 in SEQ ID NO: 9.
30. The method of claim 1, wherein the one or more polymorphic variations are detected at one or more positions in linkage disequilibrium with one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27. ,
31. The method of claim 1, wherein detecting the presence or absence of the one or more polymorphic variations comprises: hybridizing an oligonucleotide to the nucleic acid sample, wherein the oligonucleotide is complementary to a nucleotide sequence in the nucleic acid and hybridizes to a region adjacent to the polymorphic variation; extending the oligonucleotide in the presence of one or more nucleotides, yielding extension products; and . detecting the presence or absence of a polymorphic variation in the extension products.
32. The method of claim 1, wherein the subject is a human.
33. A method for identifying a polymorphic variation associated with type II diabetes proximal to an incident polymorphic variation associated with type II diabetes, which comprises: identifying a polymorphic variation proximal to the incident polymorphic variation associated with type II diabetes, wherein the polymorphic variation is detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and determining the presence or absence of an association of the proximal polymorphic variant with type II diabetes.
34. The method of claim 33, wherein the incident polymorphic variation is at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27.
35. The method of claim 33, wherein the proximal polymorphic variation is within a region between about 5 kb 5' of the incident polymorphic variation and about 5 kb 3' of the incident polymorphic variation.
36. The method of claim 33, which further comprises determining whether the proximal polymorphic variation is in linkage disequilibrium with the incident polymorphic variation.
37. The method of claim 33, which further comprises identifying a second polymorphic variation proximal to the identified proximal polymorphic variation associated with type II diabetes and determining if the second proximal polymorphic variation is associated with type II diabetes.
38. The method of claim 37, wherein the second proximal polymorphic variant is within a region between about 5 kb 5' of the incident polymorphic variation and about 5 kb 3' of the proximal polymorphic variation associated with type II diabetes.
39. An isolated nucleic acid which comprises a thymine at a position corresponding to position 19890 in SEQ ID NO: 5.
40. An oligonucleotide comprising a nucleotide sequence complementary to a portion of the nucleic acid of claim 39, wherein the 3' end of the oligonucleotide is adjacent to position 19890.
41. A microarray comprising a nucleic acid of claim 39 linked to a solid support.
42. An isolated polypeptide encoded by the nucleic acid sequence of claim 39.
43. A method for identifying a candidate molecule that increases glucose uptake in a cell, which comprises:
(a) introducing a test molecule to a system which comprises a nucleic acid comprising a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; or introducing a test molecule to a system which comprises a protein encoded by a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and
(b) determining the presence or absence, of an interaction between the test molecule and the nucleic acid or protein, whereby the presence of an interaction between the test molecule and the nucleic acid or protein identifies the test molecule as a candidate molecule that modulates cell proliferation.
44. The method of claim 43, wherein the systemls an animal.
45. The method of claim 43, wherein the system is a cell.
46. The method of claim 43, wherein the nucleotide sequence comprises one or more polymorphic variations associated with type II diabetes.
47. The method of claim 46, wherein the one or more polymorphic variations associated with type II diabetes are at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or
27.
48. A method for treating type II diabetes in a subject, which comprises administering a candidate molecule identified by the method of claim 43 to a subject in need thereof, whereby the candidate molecule treats type II diabetes in the subject.
49. A method for identifying a candidate therapeutic for treating type II diabetes, which comprises:
(a) introducing a test molecule to a system which comprises a nucleic acid comprising a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; or introducing a test molecule to a system which comprises a protein encoded by a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and
(b) determining the presence or absence of an interaction between the test molecule and the nucleic acid or protein, whereby the presence of an interaction between the test molecule and the nucleic acid or protein identifies the test molecule as a candidate therapeutic for treating type II diabetes.
50. A method for treating type II diabetes in a subject, which comprises contacting one or more cells of a subject in need thereof with a nucleic acid, wherein the nucleic acid comprises a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof, a fragment of the foregoing, or a complementary nucleotide sequence of the foregoing; whereby contacting the one or more cells of the subject with the nucleic acid treats type II diabetes in the subject.
51. The method of claim 50, wherein the nucleic acid is RNA or PNA.
52. The method of claim 50, wherein the nucleic acid is duplex RNA.
53. A method for treating type II diabetes in a subject, which comprises contacting one or more cells of a subject in need thereof with a protein, wherein the protein is encoded by a nucleotide sequence which comprises a polynucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; whereby contacting the one or more cells of the subject with the protein treats type II diabetes in the subject.
54. A method for treating type II diabetes in a subject, which comprises: detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in a nucleic acid sample from a subject, wherein the one or more polymorphic variation are detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and administering a type II diabetes treatment to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample.
55. The method of claim 54, wherein the one or more polymorphic variations are detected at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27.
56. The method of claim 54, which further comprises determining blood glucose levels in the subject.
57. The method of claim 54, wherein the treatment is selected from the group consisting of administering insulin, a hypoglycemic, a starch blocker, a liver glucose regulating agent, an insulin sensitizer, a glucose level monitoring regimen, dietary counseling, a dietary regimen for managing blood glucose levels, and combinations of the foregoing.
58. A method for selecting a therapeutic treatment for treating type II diabetes in a subject, which comprises: detecting the presence or absence of one or more polymorphic variations associated with type II diabetes, in a nucleic acid sample from a subject, wherein the one or more polymorphic variation are detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and selecting and administering a type II diabetes treatment to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample.
59. The method of claim 58, wherein the one or more polymorphic variations are detected at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27.
60. The method of claim 58, wherein a therapeutic treatment is not selected based upon the presence of one or more polymorphic variations in the nucleic acid sample.
61. The method of claim 60, wherein metformin is not selected.
62. The method of claim 61, wherein metformin is not selected when a cytosine at position 50164 is present in SEQ ID NO: 1 in the nucleic acid sample.
63. A method for detecting or preventing type II diabetes in a subject, which comprises: detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing] and administering a type II diabetes treatment or detection procedure to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample.
64. The method of claim 63, wherein the one or more polymorphic variations are detected at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27.
65. The method of claim 63, wherein the type II diabetes treatment is selected from the group consisting of administering insulin, a hypoglycemic, a starch blocker, a liver glucose regulating agent, an insulin sensitizer, a glucose level monitoring regimen, dietary counseling, a dietary regimen for managing blood glucose levels, and combinations of the foregoing.
66. A method of targeting information for preventing or treating type II diabetes to a subject in need thereof, which comprises: detecting the presence or absence of one or more polymorphic variations associated with type II diabetes in a nucleic acid sample from a subject, wherein the polymorphic variation is detected in a nucleotide sequence in SEQ ID NO: 1-9, a substantially identical sequence thereof or a fragment of the foregoing; and directing information for preventing or treating type II diabetes to a subject in need thereof based upon the presence or absence of the one or more polymorphic variations in the nucleic acid sample.
67. The method of claim 66, wherein the one or more polymorphic variations are detected at one or more positions in claim 3, 6, 9, 12, 15, 18, 21, 24 or 27.
68. The method of claim 66, wherein the information comprises a description of a type II diabetes detection procedure or treatment.
69. The method of claim 66, wherein the treatment is selected from the group consisting of administering insulin, a hypoglycemic, a starch blocker, a liver glucose regulating agent, an insulin sensitizer, a glucose level monitoring regimen, dietary counseling, a dietary regimen for managing blood glucose levels, and combinations of the foregoing.
70. A composition comprising a cell from a subject having type II diabetes or at risk of type II diabetes and an antibody that specifically binds to a protein, polypeptide or peptide encoded by a nucleotide sequence identical to or 90% or more identical to a nucleotide sequence in SEQ ID NO: 1-20.
71. The composition of claim 70, wherein the antibody specifically binds to an epitope comprising a glycine at position 557 in a ZNF221 polypeptide.
72. A composition comprising a cell from a subject having type II diabetes or at risk of type II diabetes and a RNA, DNA, PNA or ribo∑yme molecule comprising a nucleotide sequence identical to or 90% or more identical to a portion of a nucleotide sequence in SEQ ID NO: 1-20 or nucleotide sequence complementary to the foregoing.
73. The composition of claim 72, wherein the RNA molecule is a short inhibitory RNA molecule.
74. A method of genotyping a nucleic acid which comprises determining the nucleotide corresponding to position 19890 of SEQ ID NO: 5 in the nucleic acid.
PCT/US2004/023933 2004-07-22 2004-07-22 Methods for identifying risk of type ii diabetes and treatments thereof WO2006022636A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/US2004/023933 WO2006022636A1 (en) 2004-07-22 2004-07-22 Methods for identifying risk of type ii diabetes and treatments thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2004/023933 WO2006022636A1 (en) 2004-07-22 2004-07-22 Methods for identifying risk of type ii diabetes and treatments thereof

Publications (1)

Publication Number Publication Date
WO2006022636A1 true WO2006022636A1 (en) 2006-03-02

Family

ID=35967748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/023933 WO2006022636A1 (en) 2004-07-22 2004-07-22 Methods for identifying risk of type ii diabetes and treatments thereof

Country Status (1)

Country Link
WO (1) WO2006022636A1 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008087209A1 (en) * 2007-01-19 2008-07-24 Integragen Human diabetes susceptibility iglc gene
EP2021502A1 (en) * 2006-05-09 2009-02-11 Oy Jurilab Ltd Novel genes and markers in type 2 diabetes and obesity
US9233204B2 (en) 2014-01-31 2016-01-12 Aseko, Inc. Insulin management
US9483619B2 (en) 2012-09-11 2016-11-01 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US9486580B2 (en) 2014-01-31 2016-11-08 Aseko, Inc. Insulin management
US9886556B2 (en) 2015-08-20 2018-02-06 Aseko, Inc. Diabetes management therapy advisor
US9892234B2 (en) 2014-10-27 2018-02-13 Aseko, Inc. Subcutaneous outpatient management
US9897565B1 (en) 2012-09-11 2018-02-20 Aseko, Inc. System and method for optimizing insulin dosages for diabetic subjects
US11081226B2 (en) 2014-10-27 2021-08-03 Aseko, Inc. Method and controller for administering recommended insulin dosages to a patient

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE SNP [online] 27 July 2000 (2000-07-27), XP002994324, accession no. NCBI Database accession no. (RS662138) *
WANG D-S. ET AL: "Involvement of Organic Cation Transporter 1 in Hepatic Intestinal Distribution of Metformin", THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 302, no. 2, 2002, pages 510 - 515, XP002994325 *

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2021502A1 (en) * 2006-05-09 2009-02-11 Oy Jurilab Ltd Novel genes and markers in type 2 diabetes and obesity
EP2021502A4 (en) * 2006-05-09 2010-08-25 Mas Metabolic Analytical Servi Novel genes and markers in type 2 diabetes and obesity
WO2008087209A1 (en) * 2007-01-19 2008-07-24 Integragen Human diabetes susceptibility iglc gene
US10629294B2 (en) 2012-09-11 2020-04-21 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US9483619B2 (en) 2012-09-11 2016-11-01 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US11733196B2 (en) 2012-09-11 2023-08-22 Aseko, Inc. System and method for optimizing insulin dosages for diabetic subjects
US9897565B1 (en) 2012-09-11 2018-02-20 Aseko, Inc. System and method for optimizing insulin dosages for diabetic subjects
US10410740B2 (en) 2012-09-11 2019-09-10 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US11131643B2 (en) 2012-09-11 2021-09-28 Aseko, Inc. Method and system for optimizing insulin dosages for diabetic subjects
US9773096B2 (en) 2012-09-11 2017-09-26 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US9811638B2 (en) 2012-09-11 2017-11-07 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US10102922B2 (en) 2012-09-11 2018-10-16 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US9965596B2 (en) 2012-09-11 2018-05-08 Aseko, Inc. Means and method for improved glycemic control for diabetic patients
US9710611B2 (en) 2014-01-31 2017-07-18 Aseko, Inc. Insulin management
US10453568B2 (en) 2014-01-31 2019-10-22 Aseko, Inc. Method for managing administration of insulin
US9898585B2 (en) 2014-01-31 2018-02-20 Aseko, Inc. Method and system for insulin management
US9892235B2 (en) 2014-01-31 2018-02-13 Aseko, Inc. Insulin management
US9965595B2 (en) 2014-01-31 2018-05-08 Aseko, Inc. Insulin management
US11857314B2 (en) 2014-01-31 2024-01-02 Aseko, Inc. Insulin management
US11783945B2 (en) 2014-01-31 2023-10-10 Aseko, Inc. Method and system for insulin infusion rate management
US10255992B2 (en) 2014-01-31 2019-04-09 Aseko, Inc. Insulin management
US11783946B2 (en) 2014-01-31 2023-10-10 Aseko, Inc. Method and system for insulin bolus management
US11621074B2 (en) 2014-01-31 2023-04-04 Aseko, Inc. Insulin management
US9604002B2 (en) 2014-01-31 2017-03-28 Aseko, Inc. Insulin management
US11804300B2 (en) 2014-01-31 2023-10-31 Aseko, Inc. Insulin management
US10535426B2 (en) 2014-01-31 2020-01-14 Aseko, Inc. Insulin management
US9504789B2 (en) 2014-01-31 2016-11-29 Aseko, Inc. Insulin management
US10811133B2 (en) 2014-01-31 2020-10-20 Aseko, Inc. System for administering insulin boluses to a patient
US11081233B2 (en) 2014-01-31 2021-08-03 Aseko, Inc. Insulin management
US9486580B2 (en) 2014-01-31 2016-11-08 Aseko, Inc. Insulin management
US9233204B2 (en) 2014-01-31 2016-01-12 Aseko, Inc. Insulin management
US11158424B2 (en) 2014-01-31 2021-10-26 Aseko, Inc. Insulin management
US11311213B2 (en) 2014-01-31 2022-04-26 Aseko, Inc. Insulin management
US11468987B2 (en) 2014-01-31 2022-10-11 Aseko, Inc. Insulin management
US11490837B2 (en) 2014-01-31 2022-11-08 Aseko, Inc. Insulin management
US10403397B2 (en) 2014-10-27 2019-09-03 Aseko, Inc. Subcutaneous outpatient management
US11678800B2 (en) 2014-10-27 2023-06-20 Aseko, Inc. Subcutaneous outpatient management
US11694785B2 (en) 2014-10-27 2023-07-04 Aseko, Inc. Method and dosing controller for subcutaneous outpatient management
US11081226B2 (en) 2014-10-27 2021-08-03 Aseko, Inc. Method and controller for administering recommended insulin dosages to a patient
US10128002B2 (en) 2014-10-27 2018-11-13 Aseko, Inc. Subcutaneous outpatient management
US9892234B2 (en) 2014-10-27 2018-02-13 Aseko, Inc. Subcutaneous outpatient management
US11574742B2 (en) 2015-08-20 2023-02-07 Aseko, Inc. Diabetes management therapy advisor
US10380328B2 (en) 2015-08-20 2019-08-13 Aseko, Inc. Diabetes management therapy advisor
US9886556B2 (en) 2015-08-20 2018-02-06 Aseko, Inc. Diabetes management therapy advisor

Similar Documents

Publication Publication Date Title
AU2017267184B2 (en) Method for assessing a prognosis and predicting the response of patients with malignant diseases to immunotherapy
KR101708544B1 (en) Methods and nucleic acids for analyses of cellular proliferative disorders
US20230056182A1 (en) Use of adeno-associated viral vectors to correct gene defects/ express proteins in hair cells and supporting cells in the inner ear
KR102046668B1 (en) Methods and nucleic acids for determining the prognosis of a cancer subject
CN107223159A (en) The detection of DNA from particular cell types and correlation technique
KR102149483B1 (en) Use of masitinib for treatment of cancer in patient subpopulations identified using predictor factors
US20090305284A1 (en) Methods for Identifying Risk of Breast Cancer and Treatments Thereof
AU2016351889A1 (en) Detection of foetal chromosomal aneuploidies using DNA regions that are differentially methylated between the foetus and the pregnant female
CA2936612A1 (en) Atf6 polymorphisms associated with myocardial infarction, method of detection and uses thereof
KR20220025749A (en) detection of colorectal cancer
KR20060045950A (en) Prognostic for hematological malignancy
KR20220024184A (en) detection of colorectal cancer
AU2016325030A1 (en) Novel biomarkers and methods of treating cancer
KR20130123357A (en) Methods and kits for diagnosing conditions related to hypoxia
WO2006022629A1 (en) Methods of identifying risk of type ii diabetes and treatments thereof
EP1729930A2 (en) Methods for identifying risk of osteoarthritis and treatments thereof
AU2018360287B2 (en) Method for determining the response of a malignant disease to an immunotherapy
IL179831A (en) In vitro method for detecting the presence of or predisposition to autism or to an autism spectrum disorder, and an in vitro method of selecting biologically active compounds on autism or autism spectrum disorders
KR20210144822A (en) Compounds and methods for modulating UBE3A-ATS
WO2006022636A1 (en) Methods for identifying risk of type ii diabetes and treatments thereof
KR20170116009A (en) Novel rna-biomarker signature for diagnosis of prostate cancer
WO2006022634A1 (en) Methods for identifying risk of type ii diabetes and treatments thereof
KR20230074214A (en) Methods of treating fatty liver disease
WO2018209358A2 (en) Systemic delivery of polypeptides
KR102642320B1 (en) A Composition for diagnosis of resistance to anticancer drug

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase