WO2005116271A3 - Selection of microorganisms with growth dependent upon extracytoplasmic enzymes - Google Patents

Selection of microorganisms with growth dependent upon extracytoplasmic enzymes Download PDF

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Publication number
WO2005116271A3
WO2005116271A3 PCT/US2005/018430 US2005018430W WO2005116271A3 WO 2005116271 A3 WO2005116271 A3 WO 2005116271A3 US 2005018430 W US2005018430 W US 2005018430W WO 2005116271 A3 WO2005116271 A3 WO 2005116271A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
enzymes
selection
extracytoplasmic
growth dependent
Prior art date
Application number
PCT/US2005/018430
Other languages
French (fr)
Other versions
WO2005116271A2 (en
Inventor
Zhilian Fan
John Mcbride
Lee R Lynd
Original Assignee
Dartmouth College
Zhilian Fan
John Mcbride
Lee R Lynd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dartmouth College, Zhilian Fan, John Mcbride, Lee R Lynd filed Critical Dartmouth College
Publication of WO2005116271A2 publication Critical patent/WO2005116271A2/en
Publication of WO2005116271A3 publication Critical patent/WO2005116271A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells

Abstract

A method for selection of microorganisms with improved growth on non-native substrates is disclosed. Strains expressing tethered, enzymes on their cell surface are grown on non-native substrates and mutants with improved growth characteristics relative to the parent strain can be advantageously selected. Both soluble and insoluble substrates may be employed.
PCT/US2005/018430 2004-05-25 2005-05-25 Selection of microorganisms with growth dependent upon extracytoplasmic enzymes WO2005116271A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57414704P 2004-05-25 2004-05-25
US60/574,147 2004-05-25

Publications (2)

Publication Number Publication Date
WO2005116271A2 WO2005116271A2 (en) 2005-12-08
WO2005116271A3 true WO2005116271A3 (en) 2006-02-16

Family

ID=35207861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/018430 WO2005116271A2 (en) 2004-05-25 2005-05-25 Selection of microorganisms with growth dependent upon extracytoplasmic enzymes

Country Status (1)

Country Link
WO (1) WO2005116271A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7194112B2 (en) 2017-03-13 2022-12-21 ラレマンド ハンガリー リクィディティー マネジメント エルエルシー Recombinant Yeast Host Cells Expressing Cell-associated Heterologous Proteins

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2724036C (en) 2008-05-11 2018-09-25 Mascoma Corporation Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases
CN108315272A (en) 2008-11-21 2018-07-24 拉勒曼德匈牙利流动性管理有限责任公司 For using cellulose to carry out the yeast of synchronous glycosylation and the expression cellulase of fermentation
EP3596208A1 (en) * 2017-03-13 2020-01-22 Lallemand Hungary Liquidity Management LLC Recombinant yeast host cells expressing cell-associated heterologous proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
FAN Z ET AL: "Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymes.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 92, no. 1, 5 October 2005 (2005-10-05), pages 35 - 44, XP002353624, ISSN: 0006-3592 *
FRANCIS J C ET AL: "Directed evolution of metabolic pathways in microbial populations. I. Modification of the acid phosphatase pH optimum in S. cerevisiae.", GENETICS, vol. 70, no. 1, January 1972 (1972-01-01), pages 59 - 73, XP002353621, ISSN: 0016-6731 *
LEE S Y ET AL: "Microbial cell-surface display", TRENDS IN BIOTECHNOLOGY, vol. 21, no. 1, January 2003 (2003-01-01), pages 45 - 52, XP004397636, ISSN: 0167-7799 *
LELIEVELD H L M: "The use of continuous cultures for selection and isolation of microorganisms producing extracellular enzymes adapted to extreme environments", BIOTECHNOLOGY AND BIOENGINEERING, vol. 24, no. 6, 1982, pages 1419 - 1426, XP002353623, ISSN: 0006-3592 *
MURAI T ET AL: "Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing beta-glucosidase and carboxymethylcellulase from Aspergillus aculeatus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 12, December 1998 (1998-12-01), pages 4857 - 4861, XP002942424, ISSN: 0099-2240 *
MURAI T ET AL: "Construction of a starch-utilizing yeast by cell surface engineering", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 4, 1997, pages 1362 - 1366, XP002945516, ISSN: 0099-2240 *
MURAI T ET AL: "Genetic immobilization of cellulase on the cell surface of Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 48, no. 4, October 1997 (1997-10-01), pages 499 - 503, XP002353622, ISSN: 0175-7598 *
NAKI D ET AL: "Selection of a subtilisin-hyperproducing Bacillus in a highly structured environment", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 3, March 1998 (1998-03-01), pages 290 - 294, XP000972404, ISSN: 0175-7598 *
PONCE-NOYOLA T ET AL: "Isolation of a high-specific-growth-rate mutant of Cellulomonas flavigena on sugar cane bagasse", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 42, no. 5, 1995, pages 709 - 712, XP008055582, ISSN: 0175-7598 *
PONCE-NOYOLA T ET AL: "Regulation of cellulases and xylanases from a derepressed mutant of Cellulomonas flavigena growing on sugar-cane bagasse in continuous culture", BIORESOURCE TECHNOLOGY, vol. 78, no. 3, July 2001 (2001-07-01), pages 285 - 291, XP002353620, ISSN: 0960-8524 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7194112B2 (en) 2017-03-13 2022-12-21 ラレマンド ハンガリー リクィディティー マネジメント エルエルシー Recombinant Yeast Host Cells Expressing Cell-associated Heterologous Proteins

Also Published As

Publication number Publication date
WO2005116271A2 (en) 2005-12-08

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