WO2005092013A3 - Separations platform based upon electroosmosis-driven planar chromatography - Google Patents

Separations platform based upon electroosmosis-driven planar chromatography Download PDF

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Publication number
WO2005092013A3
WO2005092013A3 PCT/US2005/009210 US2005009210W WO2005092013A3 WO 2005092013 A3 WO2005092013 A3 WO 2005092013A3 US 2005009210 W US2005009210 W US 2005009210W WO 2005092013 A3 WO2005092013 A3 WO 2005092013A3
Authority
WO
WIPO (PCT)
Prior art keywords
electroosmosis
amphiphilic
platform based
dimensional
separation
Prior art date
Application number
PCT/US2005/009210
Other languages
French (fr)
Other versions
WO2005092013A2 (en
Inventor
Wayne F Patton
Mack J Schermer
Original Assignee
Perkinelmer Las Inc
Wayne F Patton
Mack J Schermer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkinelmer Las Inc, Wayne F Patton, Mack J Schermer filed Critical Perkinelmer Las Inc
Priority to EP05725938A priority Critical patent/EP1740284A4/en
Priority to AU2005226672A priority patent/AU2005226672A1/en
Priority to CA002558305A priority patent/CA2558305A1/en
Priority to JP2007504167A priority patent/JP2007529755A/en
Publication of WO2005092013A2 publication Critical patent/WO2005092013A2/en
Publication of WO2005092013A3 publication Critical patent/WO2005092013A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • C07K1/285—Isoelectric focusing multi dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/92—Construction of the plate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/94—Development
    • G01N2030/945—Application of reagents to undeveloped plate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/58—Conditioning of the sorbent material or stationary liquid the sorbent moving as a whole

Abstract

The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.
PCT/US2005/009210 2004-03-19 2005-03-18 Separations platform based upon electroosmosis-driven planar chromatography WO2005092013A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP05725938A EP1740284A4 (en) 2004-03-19 2005-03-18 Separations platform based upon electroosmosis-driven planar chromatography
AU2005226672A AU2005226672A1 (en) 2004-03-19 2005-03-18 Separations platform based upon electroosmosis-driven planar chromatography
CA002558305A CA2558305A1 (en) 2004-03-19 2005-03-18 Separations platform based upon electroosmosis-driven planar chromatography
JP2007504167A JP2007529755A (en) 2004-03-19 2005-03-18 Separation platform based on electroosmosis driven planar chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52125004P 2004-03-19 2004-03-19
US60/521,250 2004-03-19

Publications (2)

Publication Number Publication Date
WO2005092013A2 WO2005092013A2 (en) 2005-10-06
WO2005092013A3 true WO2005092013A3 (en) 2006-08-17

Family

ID=35056736

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/009210 WO2005092013A2 (en) 2004-03-19 2005-03-18 Separations platform based upon electroosmosis-driven planar chromatography

Country Status (6)

Country Link
US (1) US20050269267A1 (en)
EP (1) EP1740284A4 (en)
JP (1) JP2007529755A (en)
AU (1) AU2005226672A1 (en)
CA (1) CA2558305A1 (en)
WO (1) WO2005092013A2 (en)

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US7641780B2 (en) * 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
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US20070187243A1 (en) * 2005-11-10 2007-08-16 Perkinelmer Life And Analytical Sciences Planar electrochromatography/thin layer chromatography separations systems
WO2007067731A2 (en) * 2005-12-08 2007-06-14 Perkinelmer Las, Inc. Micelle- and microemulsion-assisted planar separations platform for proteomics
US20070251824A1 (en) * 2006-01-24 2007-11-01 Perkinelmer Las, Inc. Multiplexed analyte quantitation by two-dimensional planar electrochromatography
US20080160629A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for off-line multidimensional concentration and separation of biomolecules
US20080156080A1 (en) * 2007-01-02 2008-07-03 Calibrant Biosystems, Inc. Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
JP5152862B2 (en) * 2008-04-04 2013-02-27 独立行政法人産業技術総合研究所 Electrophoretic medium having hydrophobic polymer membrane and electrophoretic separation method using the same
GB0812576D0 (en) * 2008-07-10 2008-08-13 United Wire Ltd Separating screens
AU2010249678B2 (en) * 2009-05-19 2014-08-28 The Regents Of The University Of California Multi-directional microfluidic devices and methods
DE102009040180A1 (en) * 2009-09-07 2011-03-17 Dionex Benelux B.V. Flow control in spatial chromatography
DE102009040186A1 (en) * 2009-09-07 2011-03-24 Dionex Benelux B.V. chromatographic
JP2012093092A (en) * 2009-11-05 2012-05-17 Nagoya Institute Of Technology Planer column, and separation system and separation method using the same
GB2479394A (en) * 2010-04-08 2011-10-12 Daoyi Chen Measurement of isotopes using zeta potential
US9103779B2 (en) * 2010-04-27 2015-08-11 Dgel Electrosystem Inc. Electrophoresis buffer for faster migration, improved resolution and extended shelf-life
US8735858B2 (en) * 2010-04-30 2014-05-27 Hewlett-Packard Development Company, L.P. Ionic devices with interacting species
WO2012071472A2 (en) 2010-11-23 2012-05-31 The Regents Of The University Of California Multi-directional microfluidic devices comprising a pan-capture binding region and methods of using the same
EP2646832B1 (en) 2010-12-03 2019-09-11 The Regents of The University of California Protein renaturation microfluidic devices and methods of making and using the same
JP6141272B2 (en) * 2011-07-14 2017-06-07 ディージェル エレクトロシステム インコーポレイティッドＤｇｅｌ Ｅｌｅｃｔｒｏｓｙｓｔｅｍ Ｉｎｃ． Electrophoresis buffer for extending electrophoresis lifetime of electrophoresis gel
US9841417B2 (en) 2011-09-30 2017-12-12 The Regents Of The University Of California Microfluidic devices and methods for assaying a fluid sample using the same
FI126122B (en) * 2011-12-22 2016-06-30 Upm Kymmene Corp Use of a stationary phase comprising fibril pulp in a separation process
US10393701B2 (en) 2012-10-08 2019-08-27 The Regents Of The University Of California Microfluidic methods of assaying molecule switching and devices for practicing the same
MX346385B (en) 2013-02-14 2017-03-16 Nanopareil Llc Hybrid felts of electrospun nanofibers.
US9671368B2 (en) 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
EP3124618B1 (en) * 2014-03-24 2019-05-15 Nitto Boseki Co., Ltd. Method for separating and concentrating target substance using novel cationic graft polymer
US9607816B2 (en) * 2014-12-17 2017-03-28 Micromass Uk Limited Two-dimensional separation and imaging technique for the rapid analysis of biological samples
CN110376293A (en) * 2018-04-13 2019-10-25 株式会社岛津制作所 The analysis method of metalloprotein in biological sample
US20220379237A1 (en) * 2019-09-17 2022-12-01 Arizona Board Of Regents On Behalf Of The University Of Arizona Externally modulated variable affinity chromatography

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Also Published As

Publication number Publication date
WO2005092013A2 (en) 2005-10-06
CA2558305A1 (en) 2005-10-06
US20050269267A1 (en) 2005-12-08
EP1740284A4 (en) 2011-04-06
JP2007529755A (en) 2007-10-25
AU2005226672A1 (en) 2005-10-06
EP1740284A2 (en) 2007-01-10

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