WO2005080556A3 - Virus purification methods - Google Patents

Virus purification methods Download PDF

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Publication number
WO2005080556A3
WO2005080556A3 PCT/EP2005/050739 EP2005050739W WO2005080556A3 WO 2005080556 A3 WO2005080556 A3 WO 2005080556A3 EP 2005050739 W EP2005050739 W EP 2005050739W WO 2005080556 A3 WO2005080556 A3 WO 2005080556A3
Authority
WO
WIPO (PCT)
Prior art keywords
virus
host cells
recombinant
recombinant virus
lysing
Prior art date
Application number
PCT/EP2005/050739
Other languages
French (fr)
Other versions
WO2005080556A9 (en
WO2005080556A2 (en
Inventor
Miranda Weggeman
Corven Emile Joannes Josep Van
Original Assignee
Crucell Holland Bv
Miranda Weggeman
Corven Emile Joannes Josep Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34878416&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2005080556(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to US10/590,427 priority Critical patent/US20070207461A1/en
Priority to AU2005214090A priority patent/AU2005214090B2/en
Priority to EP05729676A priority patent/EP1718738A2/en
Priority to NZ548495A priority patent/NZ548495A/en
Priority to DK06126383T priority patent/DK1780269T3/en
Application filed by Crucell Holland Bv, Miranda Weggeman, Corven Emile Joannes Josep Van filed Critical Crucell Holland Bv
Priority to CA2555412A priority patent/CA2555412C/en
Priority to JP2006553596A priority patent/JP2007522814A/en
Publication of WO2005080556A2 publication Critical patent/WO2005080556A2/en
Publication of WO2005080556A3 publication Critical patent/WO2005080556A3/en
Priority to KR1020067017560A priority patent/KR101211329B1/en
Publication of WO2005080556A9 publication Critical patent/WO2005080556A9/en
Priority to US12/220,828 priority patent/US8124106B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10051—Methods of production or purification of viral material

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The invention provides a method for the purification of a virus from a host cell, said method comprising the steps of: a) culturing host cells, b) infecting said host cells with a virus, c) treating the cell culture with nuclease, d) lysing said host cells to provide a lysate comprising the virus. The virus is preferably a recombinant adenovirus. The invention further provides a method for the purification of a recombinant virus expressing a heterologous protein that is capable of binding nucleic acid, comprising the steps of: a) culturing host cells, b) infecting said host cells with said recombinant virus, c) lysing said host cells to provide a lysate comprising said recombinant virus, d) subjecting the recombinant virus to anion exchange chromatography and size exclusion chromatography, characterized in that the virus containing mixture is buffer exchanged at least once with a solution comprising at least 2 M NaCl, or another salt providing an equivalent ionic strength.
PCT/EP2005/050739 2004-02-23 2005-02-21 Virus purification methods WO2005080556A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
JP2006553596A JP2007522814A (en) 2004-02-23 2005-02-21 Virus purification method
AU2005214090A AU2005214090B2 (en) 2004-02-23 2005-02-21 Virus purification methods
EP05729676A EP1718738A2 (en) 2004-02-23 2005-02-21 Virus purification methods
NZ548495A NZ548495A (en) 2004-02-23 2005-02-21 Virus purification methods
DK06126383T DK1780269T3 (en) 2004-02-23 2005-02-21 Virus Purification Methods
US10/590,427 US20070207461A1 (en) 2004-02-23 2005-02-21 Virus Purification Methods
CA2555412A CA2555412C (en) 2004-02-23 2005-02-21 Virus purification methods
KR1020067017560A KR101211329B1 (en) 2004-02-23 2006-08-30 Virus purification methods
US12/220,828 US8124106B2 (en) 2004-02-23 2008-07-28 Virus purification methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP2004050190 2004-02-23
EPPCT/EP04/050190 2004-02-23

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/590,427 A-371-Of-International US20070207461A1 (en) 2004-02-23 2005-02-21 Virus Purification Methods
US12/220,828 Division US8124106B2 (en) 2004-02-23 2008-07-28 Virus purification methods

Publications (3)

Publication Number Publication Date
WO2005080556A2 WO2005080556A2 (en) 2005-09-01
WO2005080556A3 true WO2005080556A3 (en) 2006-02-23
WO2005080556A9 WO2005080556A9 (en) 2006-10-26

Family

ID=34878416

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/050739 WO2005080556A2 (en) 2004-02-23 2005-02-21 Virus purification methods

Country Status (13)

Country Link
US (2) US20070207461A1 (en)
EP (2) EP1718738A2 (en)
JP (1) JP2007522814A (en)
KR (1) KR101211329B1 (en)
CN (2) CN1922308A (en)
AT (1) ATE435906T1 (en)
AU (1) AU2005214090B2 (en)
CA (1) CA2555412C (en)
DE (1) DE602005015332D1 (en)
DK (1) DK1780269T3 (en)
ES (1) ES2329607T3 (en)
NZ (1) NZ548495A (en)
WO (1) WO2005080556A2 (en)

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