WO2005049168A3 - Method and apparatus for pre-enrichment and recovery of cells from densified whole blood - Google Patents

Method and apparatus for pre-enrichment and recovery of cells from densified whole blood Download PDF

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Publication number
WO2005049168A3
WO2005049168A3 PCT/US2004/038609 US2004038609W WO2005049168A3 WO 2005049168 A3 WO2005049168 A3 WO 2005049168A3 US 2004038609 W US2004038609 W US 2004038609W WO 2005049168 A3 WO2005049168 A3 WO 2005049168A3
Authority
WO
WIPO (PCT)
Prior art keywords
enrichment
whole blood
target cells
cells
processor systems
Prior art date
Application number
PCT/US2004/038609
Other languages
French (fr)
Other versions
WO2005049168A2 (en
Inventor
Steven Gross
Herman Rutner
Original Assignee
Immunivest Corp
Steven Gross
Herman Rutner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunivest Corp, Steven Gross, Herman Rutner filed Critical Immunivest Corp
Publication of WO2005049168A2 publication Critical patent/WO2005049168A2/en
Publication of WO2005049168A3 publication Critical patent/WO2005049168A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation

Abstract

The present invention describes reagents, methods and apparatus for pre-processing of blood samples to obtain blood fractions containing substantially all target cells in reduced specimen volumes that are suitable for subsequent further enrichment. Sample processor systems are described for enriching cell populations having densities of less than approximately 1.09 g/ml from about 30 ml whole blood. Whole blood is combined with a cell compatible dense reagent to raise the density to about 1.09 g/ml. Centrifugation causes the target cells and most of the WBC in the buffy coat layer to accumulate at the top under a low-density buffer overlay. The reduced sample volumes are suitable for subsequent immunomagnetic or other enrichment methods prior to detection and enumeration. These processor systems, in conjunction with other enrichment procedures, facilitate detection of rare target cells, such as circulating tumor cells (CTC) that are useful markers in cancer diagnosis and monitoring of therapeutic response.
PCT/US2004/038609 2003-11-17 2004-11-17 Method and apparatus for pre-enrichment and recovery of cells from densified whole blood WO2005049168A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52061403P 2003-11-17 2003-11-17
US60/520,614 2003-11-17

Publications (2)

Publication Number Publication Date
WO2005049168A2 WO2005049168A2 (en) 2005-06-02
WO2005049168A3 true WO2005049168A3 (en) 2005-09-09

Family

ID=34619495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/038609 WO2005049168A2 (en) 2003-11-17 2004-11-17 Method and apparatus for pre-enrichment and recovery of cells from densified whole blood

Country Status (1)

Country Link
WO (1) WO2005049168A2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles

Families Citing this family (10)

* Cited by examiner, † Cited by third party
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US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
EP2029779A4 (en) 2006-06-14 2010-01-20 Living Microsystems Inc Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US8889361B2 (en) * 2007-09-19 2014-11-18 The Research Foundation For The State University Of New York Gene expression signatures in enriched tumor cell samples
US10022731B2 (en) * 2012-01-31 2018-07-17 Argos Therapeutics, Inc. Centrifuge vessels with a center mound and an internal elongate tube suitable for live cell processing and associated systems and methods
US20150328260A1 (en) * 2012-12-18 2015-11-19 Robert Chow Blood cell preparations and related methods (gen 8)
CN113512522A (en) 2013-03-15 2021-10-19 普林斯顿大学理事会 Method and apparatus for high throughput purification
CN105264127B (en) 2013-03-15 2019-04-09 Gpb科学有限责任公司 The on piece microfluidic process of particle
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration

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US5645715A (en) * 1995-05-02 1997-07-08 Biocomp Instruments Inc. Collection tip for fractionating solution gradients

Patent Citations (1)

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US5645715A (en) * 1995-05-02 1997-07-08 Biocomp Instruments Inc. Collection tip for fractionating solution gradients

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BAKER M.K. ET AL: "Molecular Detection of Breast Cancer Cells in the Peripheral Blood of Advanced-Stage Breast Cancer Patients Using Multimarker Real-Time Reverse Transcription-Polymerase Chain Reaction and a Novel Porous Barrier Density Gradient Centrifugation Technology", CLINICAL CANCER RESEARCH, vol. 9, no. 13, 15 October 2003 (2003-10-15), pages 4865 - 4871, XP002988215 *
GRAHAM M.K. ET AL: "Isolation of Human Polymorphonuclear Leukocytes (Granulocytes) from a Leukocyte-Rich Fraction", THE SCIENTIFIC WORLD JOURNAL, vol. 2, 2002, pages 1393 - 1396, XP009112861 *
HARDINGHAM J.E. ET AL: "Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction", CANCER RESEARCH, vol. 53, no. 15, 15 October 2003 (2003-10-15), pages 3455 - 3458, XP000605489 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
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