WO2005003291A3 - Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries - Google Patents
Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries Download PDFInfo
- Publication number
- WO2005003291A3 WO2005003291A3 PCT/US2003/032218 US0332218W WO2005003291A3 WO 2005003291 A3 WO2005003291 A3 WO 2005003291A3 US 0332218 W US0332218 W US 0332218W WO 2005003291 A3 WO2005003291 A3 WO 2005003291A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- phosphorodithioate
- bead bound
- aptamer libraries
- beads
- bound combinatorial
- Prior art date
Links
- 239000011324 bead Substances 0.000 title abstract 5
- 108091023037 Aptamer Proteins 0.000 title abstract 3
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 title abstract 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 title abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 abstract 1
- 108091008104 nucleic acid aptamers Proteins 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
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Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2003304278A AU2003304278B2 (en) | 2002-10-16 | 2003-10-14 | Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries |
EP03816480A EP1572978A4 (en) | 2002-10-16 | 2003-10-14 | Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries |
CA002502610A CA2502610A1 (en) | 2002-10-16 | 2003-10-14 | Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27250902A | 2002-10-16 | 2002-10-16 | |
US10/272,509 | 2002-10-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005003291A2 WO2005003291A2 (en) | 2005-01-13 |
WO2005003291A3 true WO2005003291A3 (en) | 2005-08-18 |
Family
ID=33563566
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/032218 WO2005003291A2 (en) | 2002-10-16 | 2003-10-14 | Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries |
Country Status (5)
Country | Link |
---|---|
US (5) | US7338762B2 (en) |
EP (1) | EP1572978A4 (en) |
AU (1) | AU2003304278B2 (en) |
CA (2) | CA2749227A1 (en) |
WO (1) | WO2005003291A2 (en) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1572978A4 (en) | 2002-10-16 | 2006-05-24 | Univ Texas | Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries |
WO2005018537A2 (en) * | 2003-05-23 | 2005-03-03 | Board Of Regents - The University Of Texas System | Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting ap-1 transcription factors |
US7910523B2 (en) | 2003-05-23 | 2011-03-22 | Board Of Regents, The University Of Texas System | Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors |
US7576037B2 (en) | 2005-11-18 | 2009-08-18 | Mei Technologies, Inc. | Process and apparatus for combinatorial synthesis |
US8084734B2 (en) * | 2006-05-26 | 2011-12-27 | The George Washington University | Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays |
US20100151465A1 (en) | 2008-03-27 | 2010-06-17 | Jingyue Ju | Selective Capture and Release of Analytes |
WO2008134077A1 (en) * | 2007-04-30 | 2008-11-06 | Archemix Corp. | Metabolic profile directed aptamer medicinal chemistry |
GB2452239B (en) * | 2007-06-01 | 2012-08-29 | Kratos Analytical Ltd | Method and apparatus useful for imaging |
PT2209893E (en) * | 2007-10-12 | 2014-02-26 | Pronota Nv | Use of aptamers in proteomics |
WO2010036866A1 (en) | 2008-09-25 | 2010-04-01 | University Of Guelph | Nitrogen responsive early nodulin gene |
CN101768213B (en) | 2008-12-30 | 2012-05-30 | 中国科学院遗传与发育生物学研究所 | Protein related to plant tillering number and coding gene and application thereof |
US8110796B2 (en) | 2009-01-17 | 2012-02-07 | The George Washington University | Nanophotonic production, modulation and switching of ions by silicon microcolumn arrays |
US8741558B2 (en) | 2009-02-04 | 2014-06-03 | Wake Forest University | Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries |
CN101817879A (en) | 2009-02-26 | 2010-09-01 | 中国科学院遗传与发育生物学研究所 | Metallothionein and encoding gene and application thereof |
US9490113B2 (en) * | 2009-04-07 | 2016-11-08 | The George Washington University | Tailored nanopost arrays (NAPA) for laser desorption ionization in mass spectrometry |
US9090663B2 (en) * | 2009-04-21 | 2015-07-28 | The Trustees Of Columbia University In The City Of New York | Systems and methods for the capture and separation of microparticles |
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AU2003304278A1 (en) | 2005-01-21 |
CA2502610A1 (en) | 2005-01-13 |
US20110224099A1 (en) | 2011-09-15 |
EP1572978A2 (en) | 2005-09-14 |
US20050123939A1 (en) | 2005-06-09 |
CA2749227A1 (en) | 2005-01-13 |
US20080255005A1 (en) | 2008-10-16 |
AU2003304278B2 (en) | 2009-03-12 |
US7338762B2 (en) | 2008-03-04 |
WO2005003291A2 (en) | 2005-01-13 |
EP1572978A4 (en) | 2006-05-24 |
US20090123922A1 (en) | 2009-05-14 |
US20080200340A1 (en) | 2008-08-21 |
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