WO2004071155A3 - Solid phase sequencing - Google Patents

Solid phase sequencing Download PDF

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Publication number
WO2004071155A3
WO2004071155A3 PCT/US2004/003283 US2004003283W WO2004071155A3 WO 2004071155 A3 WO2004071155 A3 WO 2004071155A3 US 2004003283 W US2004003283 W US 2004003283W WO 2004071155 A3 WO2004071155 A3 WO 2004071155A3
Authority
WO
WIPO (PCT)
Prior art keywords
polyphosphate
nucleic acid
label
phosphate
analogue
Prior art date
Application number
PCT/US2004/003283
Other languages
French (fr)
Other versions
WO2004071155A2 (en
Inventor
Anup Sood
Shiv Kumar
John Nelson
Carl Fuller
Original Assignee
Amersham Biosciences Corp
Anup Sood
Shiv Kumar
John Nelson
Carl Fuller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp, Anup Sood, Shiv Kumar, John Nelson, Carl Fuller filed Critical Amersham Biosciences Corp
Priority to CN200480003559.4A priority Critical patent/CN101384729B/en
Priority to JP2006503341A priority patent/JP4896707B2/en
Priority to EP04708591A priority patent/EP1597602A4/en
Priority to CA002513690A priority patent/CA2513690A1/en
Priority to AU2004211920A priority patent/AU2004211920B2/en
Publication of WO2004071155A2 publication Critical patent/WO2004071155A2/en
Priority to IL169535A priority patent/IL169535A/en
Publication of WO2004071155A3 publication Critical patent/WO2004071155A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present invention describes methods of sequencing a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. In some instances the labeled polyphosphate may be detected directly via the label and provide information on the nucleic acid. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and characterization of a target nucleic acid.
PCT/US2004/003283 2003-02-05 2004-02-05 Solid phase sequencing WO2004071155A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN200480003559.4A CN101384729B (en) 2003-02-05 2004-02-05 Solid phase sequencing
JP2006503341A JP4896707B2 (en) 2003-02-05 2004-02-05 Solid phase sequencing
EP04708591A EP1597602A4 (en) 2003-02-05 2004-02-05 Solid phase sequencing
CA002513690A CA2513690A1 (en) 2003-02-05 2004-02-05 Solid phase sequencing
AU2004211920A AU2004211920B2 (en) 2003-02-05 2004-02-05 Solid phase sequencing
IL169535A IL169535A (en) 2003-02-05 2005-07-05 Method for solid phase sequencing of target

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44519303P 2003-02-05 2003-02-05
US60/445,193 2003-02-05

Publications (2)

Publication Number Publication Date
WO2004071155A2 WO2004071155A2 (en) 2004-08-26
WO2004071155A3 true WO2004071155A3 (en) 2008-08-21

Family

ID=32869321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/003283 WO2004071155A2 (en) 2003-02-05 2004-02-05 Solid phase sequencing

Country Status (8)

Country Link
US (1) US20040152119A1 (en)
EP (1) EP1597602A4 (en)
JP (1) JP4896707B2 (en)
CN (1) CN101384729B (en)
AU (1) AU2004211920B2 (en)
CA (1) CA2513690A1 (en)
IL (1) IL169535A (en)
WO (1) WO2004071155A2 (en)

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Also Published As

Publication number Publication date
AU2004211920B2 (en) 2009-05-14
IL169535A0 (en) 2007-07-04
EP1597602A2 (en) 2005-11-23
AU2004211920A1 (en) 2004-08-26
CA2513690A1 (en) 2004-08-26
CN101384729A (en) 2009-03-11
IL169535A (en) 2011-07-31
JP2007524359A (en) 2007-08-30
US20040152119A1 (en) 2004-08-05
EP1597602A4 (en) 2009-07-22
CN101384729B (en) 2014-09-10
WO2004071155A2 (en) 2004-08-26
JP4896707B2 (en) 2012-03-14

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