WO2004031721A3 - Detection of biological threat agents - Google Patents

Detection of biological threat agents Download PDF

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Publication number
WO2004031721A3
WO2004031721A3 PCT/US2003/029588 US0329588W WO2004031721A3 WO 2004031721 A3 WO2004031721 A3 WO 2004031721A3 US 0329588 W US0329588 W US 0329588W WO 2004031721 A3 WO2004031721 A3 WO 2004031721A3
Authority
WO
WIPO (PCT)
Prior art keywords
probe
affinity reagent
methods
sensor
identity
Prior art date
Application number
PCT/US2003/029588
Other languages
French (fr)
Other versions
WO2004031721A2 (en
Inventor
Phillip D Barnes
Leonard Brillson
Stephen C Lee
Original Assignee
Univ Ohio State Res Found
Phillip D Barnes
Leonard Brillson
Stephen C Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ohio State Res Found, Phillip D Barnes, Leonard Brillson, Stephen C Lee filed Critical Univ Ohio State Res Found
Priority to EP03796339A priority Critical patent/EP1549785A2/en
Priority to CA002503163A priority patent/CA2503163A1/en
Priority to AU2003298588A priority patent/AU2003298588A1/en
Publication of WO2004031721A2 publication Critical patent/WO2004031721A2/en
Publication of WO2004031721A3 publication Critical patent/WO2004031721A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated 'L', 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagent-analyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof. Methods and devices are provided for detecting and confirming the identity of analytes such as threat agents, particularly analytes which are air or fluid borne. The methods comprise providing an affinity reagent having a known charge density in a medium which is capable of conducting electrical current, and the devices comprise a sensor as depicted in the figure below. Figure 4 (a) depicts the probe module portion of the sensor, comprising a bulb (26), delivery tubes (27) and probe chambers (28), and is connected to the power sources (batteries, 24) and the LED (designated 'L', 25). The chip (29) comprising an array of discrete transistors (30) is connected to the probe module to enable deployment of the probe reagents to the transistor array. Analyte identity is probed by manipulation of the charge density of the affinity reagent-analyte complex, including methods comprising altering probe conditions for different pH solutions, charge, a secondary affinity reagent, or combinations thereof.
PCT/US2003/029588 2002-09-20 2003-09-22 Detection of biological threat agents WO2004031721A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03796339A EP1549785A2 (en) 2002-09-20 2003-09-22 Detection of biological threat agents
CA002503163A CA2503163A1 (en) 2002-09-20 2003-09-22 Detection of biological threat agents
AU2003298588A AU2003298588A1 (en) 2002-09-20 2003-09-22 Detection of biological threat agents

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41263202P 2002-09-20 2002-09-20
US60/412,632 2002-09-20

Publications (2)

Publication Number Publication Date
WO2004031721A2 WO2004031721A2 (en) 2004-04-15
WO2004031721A3 true WO2004031721A3 (en) 2004-07-01

Family

ID=32069712

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/029588 WO2004031721A2 (en) 2002-09-20 2003-09-22 Detection of biological threat agents

Country Status (4)

Country Link
EP (1) EP1549785A2 (en)
AU (1) AU2003298588A1 (en)
CA (1) CA2503163A1 (en)
WO (1) WO2004031721A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569850B2 (en) 2005-01-24 2009-08-04 Lawrence Livermore National Security, Llc Lipid bilayers on nano-templates
US7544978B2 (en) 2005-01-24 2009-06-09 Lawrence Livermore National Security, Llc Lipid nanotube or nanowire sensor
DE102007054691A1 (en) * 2007-11-14 2009-05-20 Leibniz-Institut Für Neue Materialien Gemeinnützige Gmbh Use of nanostructured surfaces and methods for enriching or isolating cellular subpopulations
CN102937673A (en) * 2012-11-25 2013-02-20 中国航天科技集团公司第五研究院第五一〇研究所 Method for detecting surface charge density of dielectric material under electron irradiation
CN109752417B (en) * 2018-12-25 2021-09-03 中国科学院上海微系统与信息技术研究所 Method for detecting diethyl chlorophosphonate gas and/or sarin poison gas based on charge transfer compound

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4490216A (en) * 1983-02-03 1984-12-25 Molecular Devices Corporation Lipid membrane electroanalytical elements and method of analysis therewith
US5466348A (en) * 1991-10-21 1995-11-14 Holm-Kennedy; James W. Methods and devices for enhanced biochemical sensing
US5827482A (en) * 1996-08-20 1998-10-27 Motorola Corporation Transistor-based apparatus and method for molecular detection and field enhancement
US6123819A (en) * 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
US6175752B1 (en) * 1998-04-30 2001-01-16 Therasense, Inc. Analyte monitoring device and methods of use
US6326215B1 (en) * 1997-05-14 2001-12-04 Keensense, Inc. Molecular wire injection sensors

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4490216A (en) * 1983-02-03 1984-12-25 Molecular Devices Corporation Lipid membrane electroanalytical elements and method of analysis therewith
US5466348A (en) * 1991-10-21 1995-11-14 Holm-Kennedy; James W. Methods and devices for enhanced biochemical sensing
US5827482A (en) * 1996-08-20 1998-10-27 Motorola Corporation Transistor-based apparatus and method for molecular detection and field enhancement
US6326215B1 (en) * 1997-05-14 2001-12-04 Keensense, Inc. Molecular wire injection sensors
US6123819A (en) * 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
US6175752B1 (en) * 1998-04-30 2001-01-16 Therasense, Inc. Analyte monitoring device and methods of use

Also Published As

Publication number Publication date
EP1549785A2 (en) 2005-07-06
CA2503163A1 (en) 2004-04-15
WO2004031721A2 (en) 2004-04-15
AU2003298588A8 (en) 2004-04-23
AU2003298588A1 (en) 2004-04-23

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