WO2003078583A3 - Optimization of crossover points for directed evolution - Google Patents

Optimization of crossover points for directed evolution Download PDF

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Publication number
WO2003078583A3
WO2003078583A3 PCT/US2003/007610 US0307610W WO03078583A3 WO 2003078583 A3 WO2003078583 A3 WO 2003078583A3 US 0307610 W US0307610 W US 0307610W WO 03078583 A3 WO03078583 A3 WO 03078583A3
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WO
WIPO (PCT)
Prior art keywords
optimization
methods
directed evolution
crossover points
sequences
Prior art date
Application number
PCT/US2003/007610
Other languages
French (fr)
Other versions
WO2003078583A2 (en
Inventor
Sridhar Govindarajan
Claes Gustafsson
Jeremy S Minshull
Original Assignee
Maxygen Inc
Mundorff Emily C
Sridhar Govindarajan
Claes Gustafsson
Jeremy S Minshull
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Mundorff Emily C, Sridhar Govindarajan, Claes Gustafsson, Jeremy S Minshull filed Critical Maxygen Inc
Priority to JP2003576577A priority Critical patent/JP4851687B2/en
Priority to EP03711540A priority patent/EP1488335A4/en
Priority to AU2003213846A priority patent/AU2003213846A1/en
Publication of WO2003078583A2 publication Critical patent/WO2003078583A2/en
Publication of WO2003078583A3 publication Critical patent/WO2003078583A3/en

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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20—Protein or domain folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/20—Screening of libraries
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60—In silico combinatorial chemistry
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment

Abstract

Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.
PCT/US2003/007610 2002-03-09 2003-03-10 Optimization of crossover points for directed evolution WO2003078583A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2003576577A JP4851687B2 (en) 2002-03-09 2003-03-10 Crossover optimization for directed evolution
EP03711540A EP1488335A4 (en) 2002-03-09 2003-03-10 Optimization of crossover points for directed evolution
AU2003213846A AU2003213846A1 (en) 2002-03-09 2003-03-10 Optimization of crossover points for directed evolution

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US36350502P 2002-03-09 2002-03-09
US60/363,505 2002-03-09
US37359102P 2002-04-18 2002-04-18
US60/373,591 2002-04-18

Publications (2)

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WO2003078583A2 WO2003078583A2 (en) 2003-09-25
WO2003078583A3 true WO2003078583A3 (en) 2004-03-18

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PCT/US2003/007610 WO2003078583A2 (en) 2002-03-09 2003-03-10 Optimization of crossover points for directed evolution

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US (3) US7620500B2 (en)
EP (1) EP1488335A4 (en)
JP (3) JP4851687B2 (en)
AU (1) AU2003213846A1 (en)
WO (1) WO2003078583A2 (en)

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Also Published As

Publication number Publication date
AU2003213846A1 (en) 2003-09-29
AU2003213846A8 (en) 2003-09-29
JP4851687B2 (en) 2012-01-11
JP2011217751A (en) 2011-11-04
US8224580B2 (en) 2012-07-17
WO2003078583A2 (en) 2003-09-25
EP1488335A4 (en) 2006-11-15
EP1488335A2 (en) 2004-12-22
US8108150B2 (en) 2012-01-31
US20030198988A1 (en) 2003-10-23
US7620500B2 (en) 2009-11-17
JP2010015581A (en) 2010-01-21
US20080020397A1 (en) 2008-01-24
US20100070192A1 (en) 2010-03-18
JP2005520244A (en) 2005-07-07

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