WO2003054214A3 - Tsunami chain reaction - geometric dna amplification - Google Patents

Tsunami chain reaction - geometric dna amplification Download PDF

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Publication number
WO2003054214A3
WO2003054214A3 PCT/US2002/039063 US0239063W WO03054214A3 WO 2003054214 A3 WO2003054214 A3 WO 2003054214A3 US 0239063 W US0239063 W US 0239063W WO 03054214 A3 WO03054214 A3 WO 03054214A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
tsunami
probes
chain reaction
Prior art date
Application number
PCT/US2002/039063
Other languages
French (fr)
Other versions
WO2003054214A2 (en
Inventor
Richard V Denton
James O Bowlby Jr
Original Assignee
Richard V Denton
James O Bowlby Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Richard V Denton, James O Bowlby Jr filed Critical Richard V Denton
Priority to AU2002351276A priority Critical patent/AU2002351276A1/en
Publication of WO2003054214A2 publication Critical patent/WO2003054214A2/en
Publication of WO2003054214A3 publication Critical patent/WO2003054214A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to methods, kits and compositions for amplifying and detecting a target nucleic acid by releasing multiple free copies of probes containing a sequence in common with the target nucleic acid. This is accomplished by using at least two sets of separated probes, one which includes a portion containing a sequence complementary to the target nucleic acid, and the other which includes a portion in common with the target nucleic acid. Repeated hybridization and release of probes in the probe sets results in a rapid geometric increase in the free copies of the target nucleic acid, resulting in a capability to detect the target nucleic acid with very high sensitivity. Detection occurs using a variety of labels or other means.
PCT/US2002/039063 2001-12-10 2002-12-06 Tsunami chain reaction - geometric dna amplification WO2003054214A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002351276A AU2002351276A1 (en) 2001-12-10 2002-12-06 Tsunami chain reaction - geometric dna amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33979601P 2001-12-10 2001-12-10
US60/339,796 2001-12-10

Publications (2)

Publication Number Publication Date
WO2003054214A2 WO2003054214A2 (en) 2003-07-03
WO2003054214A3 true WO2003054214A3 (en) 2004-07-01

Family

ID=23330632

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/039063 WO2003054214A2 (en) 2001-12-10 2002-12-06 Tsunami chain reaction - geometric dna amplification

Country Status (2)

Country Link
AU (1) AU2002351276A1 (en)
WO (1) WO2003054214A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1668158B1 (en) * 2003-08-11 2010-10-20 Georgia State University Research Foundation, Inc. Rna detection and quantitation
EP2401388A4 (en) 2009-02-23 2012-12-05 Univ Georgetown Sequence-specific detection of nucleotide sequences
EP2722105A1 (en) * 2012-10-22 2014-04-23 Universität Wien Method of in situ synthesizing microarrays
GB201513128D0 (en) 2015-07-24 2015-09-09 Sense Biodetection Ltd Nucleic acid detection method
ITUB20155706A1 (en) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Nucleic acid detection procedure and related kit.
ITUB20155686A1 (en) * 2015-11-18 2017-05-18 Fondazione St Italiano Tecnologia Nucleic acid detection procedure and related kit.
AU2017361521B2 (en) 2016-11-21 2020-08-27 Nanostring Technologies, Inc. Chemical compositions and methods of using same
GB201701262D0 (en) 2017-01-25 2017-03-08 Sense Biodetection Ltd Nucleic acid detection method
SG11202011274YA (en) 2018-05-14 2020-12-30 Nanostring Technologies Inc Chemical compositions and methods of using same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5593840A (en) * 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences

Also Published As

Publication number Publication date
AU2002351276A8 (en) 2003-07-09
AU2002351276A1 (en) 2003-07-09
WO2003054214A2 (en) 2003-07-03

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