WO2003040024A3 - Assay basierend auf dotierten nanoteilchen - Google Patents

Assay basierend auf dotierten nanoteilchen Download PDF

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Publication number
WO2003040024A3
WO2003040024A3 PCT/EP2002/012256 EP0212256W WO03040024A3 WO 2003040024 A3 WO2003040024 A3 WO 2003040024A3 EP 0212256 W EP0212256 W EP 0212256W WO 03040024 A3 WO03040024 A3 WO 03040024A3
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WO
WIPO (PCT)
Prior art keywords
donor
molecule
acceptor
assay based
energy
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PCT/EP2002/012256
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English (en)
French (fr)
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WO2003040024A2 (de
Inventor
Kerstin Bohmann
Werner Hoheisel
Burkhard Koehler
Ingmar Dorn
Original Assignee
Bayer Ag
Kerstin Bohmann
Werner Hoheisel
Burkhard Koehler
Ingmar Dorn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag, Kerstin Bohmann, Werner Hoheisel, Burkhard Koehler, Ingmar Dorn filed Critical Bayer Ag
Priority to US10/494,390 priority Critical patent/US7410810B2/en
Priority to CA2465646A priority patent/CA2465646C/en
Priority to JP2003542078A priority patent/JP4233452B2/ja
Priority to EP02787546A priority patent/EP1444517B1/de
Priority to DE50210393T priority patent/DE50210393D1/de
Priority to AU2002351820A priority patent/AU2002351820B2/en
Publication of WO2003040024A2 publication Critical patent/WO2003040024A2/de
Publication of WO2003040024A3 publication Critical patent/WO2003040024A3/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Biophysics (AREA)
  • Composite Materials (AREA)
  • Pathology (AREA)
  • Materials Engineering (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Gegenstand der Erfindung ist ein Assay auf der Basis eines Resonanz-Energie-Transfers (RET), umfassend eine erste Molekülgruppe A, die mit mindestens einem Energie-Donor markiert ist, und mindestens eine zweite Molekülgruppe B, die mit mindestens einem Energie-Akzeptor markiert ist, wobei der Donor ein Molekül oder Teilchen umfasst, das durch eine äuβere Strahlungsquelle energetisch anregbar und fluoreszenzfähig ist, der Akzeptor ein Molekül oder Teilchen umfasst, das durch Energietransfer über den Donor unter teilweiser oder vollständiger Löschung der Donor-Fluoreszenz anregbar ist, und Donor und/oder Akzeptor lumineszierende anorganische dotierte Nanoteilchen mit einer Ausdehnung von ≤ 50 Nanometer umfassen, die nach einer energetischen Anregung elektromagnetische Strahlung mit einer Stokes- oder Antistokes-Verschiebung emittieren.
PCT/EP2002/012256 2001-11-05 2002-11-04 Assay basierend auf dotierten nanoteilchen WO2003040024A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US10/494,390 US7410810B2 (en) 2001-11-05 2002-11-04 Assay based on doped nanoparticles
CA2465646A CA2465646C (en) 2001-11-05 2002-11-04 Assay based on doped nanoparticles
JP2003542078A JP4233452B2 (ja) 2001-11-05 2002-11-04 ドープしたナノ粒子に基づくアッセイ
EP02787546A EP1444517B1 (de) 2001-11-05 2002-11-04 Assay basierend auf dotierten nanoteilchen
DE50210393T DE50210393D1 (de) 2001-11-05 2002-11-04 Assay basierend auf dotierten nanoteilchen
AU2002351820A AU2002351820B2 (en) 2001-11-05 2002-11-04 Assay based on doped nanoparticles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10153829A DE10153829A1 (de) 2001-11-05 2001-11-05 Assay basierend auf dotierten Nanoteilchen
DE10153829.4 2001-11-05

Publications (2)

Publication Number Publication Date
WO2003040024A2 WO2003040024A2 (de) 2003-05-15
WO2003040024A3 true WO2003040024A3 (de) 2003-10-23

Family

ID=7704386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/012256 WO2003040024A2 (de) 2001-11-05 2002-11-04 Assay basierend auf dotierten nanoteilchen

Country Status (9)

Country Link
US (1) US7410810B2 (de)
EP (1) EP1444517B1 (de)
JP (1) JP4233452B2 (de)
AT (1) ATE365917T1 (de)
AU (1) AU2002351820B2 (de)
CA (1) CA2465646C (de)
DE (2) DE10153829A1 (de)
ES (1) ES2287342T3 (de)
WO (1) WO2003040024A2 (de)

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EP1444517B1 (de) 2007-06-27
US20050064604A1 (en) 2005-03-24
EP1444517A2 (de) 2004-08-11
JP4233452B2 (ja) 2009-03-04
CA2465646A1 (en) 2003-05-15
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ES2287342T3 (es) 2007-12-16
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CA2465646C (en) 2012-04-17
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