WO2002097444A3 - Misfolded protein sensor method - Google Patents

Misfolded protein sensor method Download PDF

Info

Publication number
WO2002097444A3
WO2002097444A3 PCT/US2002/017212 US0217212W WO02097444A3 WO 2002097444 A3 WO2002097444 A3 WO 2002097444A3 US 0217212 W US0217212 W US 0217212W WO 02097444 A3 WO02097444 A3 WO 02097444A3
Authority
WO
WIPO (PCT)
Prior art keywords
probe
abnormal
proteinaceous
bind
interaction
Prior art date
Application number
PCT/US2002/017212
Other languages
French (fr)
Other versions
WO2002097444A2 (en
Inventor
Cindy Orser
Anne Grosset
Eugene Davidson
Original Assignee
Arete Associates
Cindy Orser
Anne Grosset
Eugene Davidson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP02749544A priority Critical patent/EP1395833B1/en
Priority to CA2448981A priority patent/CA2448981C/en
Priority to JP2003500572A priority patent/JP4235544B2/en
Priority to AU2002320045A priority patent/AU2002320045B2/en
Priority to US10/494,906 priority patent/US7691639B2/en
Priority to MXPA03011000A priority patent/MXPA03011000A/en
Application filed by Arete Associates, Cindy Orser, Anne Grosset, Eugene Davidson filed Critical Arete Associates
Publication of WO2002097444A2 publication Critical patent/WO2002097444A2/en
Publication of WO2002097444A3 publication Critical patent/WO2002097444A3/en
Priority to US11/979,226 priority patent/US20080171341A1/en
Priority to AU2008203542A priority patent/AU2008203542B2/en
Priority to US12/726,941 priority patent/US8062895B2/en
Priority to US14/484,683 priority patent/US9638702B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2828—Prion diseases

Abstract

A catalytic conformational sensor method for detecting abnormal proteins and proteinaceous particles. The method is based on the interaction of a peptide fragment or probe with an abnormal proteinaceous particle. The interaction catalyzes transformation of the probe to a predominately beta sheet conformation and allows the probe to bind to the abnormal proteinaceous particle. This in turn, catalyzes propagation of a signal associated with the test sample-bound probe. As a result signals can be propagated even from samples containing very low concentrations of abnormal proteinaceous particles. The peptide probes can be designed to bind to a desired peptide sequence or can even be based on dendrimer structure to control further aggregate propagation.
PCT/US2002/017212 2001-05-31 2002-05-30 Misfolded protein sensor method WO2002097444A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CA2448981A CA2448981C (en) 2001-05-31 2002-05-30 Misfolded protein sensor method
JP2003500572A JP4235544B2 (en) 2001-05-31 2002-05-30 Defect folding protein sensor method
AU2002320045A AU2002320045B2 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method
US10/494,906 US7691639B2 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method
MXPA03011000A MXPA03011000A (en) 2001-05-31 2002-05-30 Misfolded protein sensor method.
EP02749544A EP1395833B1 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method
US11/979,226 US20080171341A1 (en) 2001-05-31 2007-10-31 Detection of conformationally altered proteins and prions
AU2008203542A AU2008203542B2 (en) 2001-05-31 2008-08-07 Misfolded protein sensor method
US12/726,941 US8062895B2 (en) 2001-05-31 2010-03-18 Misfolded protein sensor method
US14/484,683 US9638702B2 (en) 2001-05-31 2014-09-12 Detection of conformationally altered proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29545601P 2001-05-31 2001-05-31
US60/295,456 2001-05-31

Related Child Applications (5)

Application Number Title Priority Date Filing Date
US10/161,061 Continuation-In-Part US7166471B2 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method in body fluids
US10494906 A-371-Of-International 2002-05-30
US10/494,906 A-371-Of-International US7691639B2 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method
US11/979,226 Continuation-In-Part US20080171341A1 (en) 2001-05-31 2007-10-31 Detection of conformationally altered proteins and prions
US12/726,941 Continuation US8062895B2 (en) 2001-05-31 2010-03-18 Misfolded protein sensor method

Publications (2)

Publication Number Publication Date
WO2002097444A2 WO2002097444A2 (en) 2002-12-05
WO2002097444A3 true WO2002097444A3 (en) 2003-10-30

Family

ID=23137808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/017212 WO2002097444A2 (en) 2001-05-31 2002-05-30 Misfolded protein sensor method

Country Status (7)

Country Link
US (4) US7166471B2 (en)
EP (1) EP1395833B1 (en)
JP (1) JP4235544B2 (en)
AU (2) AU2002320045B2 (en)
CA (1) CA2448981C (en)
MX (1) MXPA03011000A (en)
WO (1) WO2002097444A2 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4235544B2 (en) 2001-05-31 2009-03-11 アドライフ インコーポレーティッド Defect folding protein sensor method
US20050026165A1 (en) * 2001-05-31 2005-02-03 Cindy Orser Detection of conformationally altered proteins and prions
EP1380290A1 (en) 2002-07-09 2004-01-14 Universitair Medisch Centrum Utrecht Cross-beta structure pathway and its therapeutic relevance
BRPI0413495A (en) 2003-08-13 2006-10-17 Chiron Corp prion-specific peptide reagents and their uses
US20060035242A1 (en) 2004-08-13 2006-02-16 Michelitsch Melissa D Prion-specific peptide reagents
WO2005093430A1 (en) * 2004-03-25 2005-10-06 Fuence Co., Ltd. Method of detecting amyloid structural change in protein, method of searching for substance having activity of affecting amyloid structural change and method of searching for remedy or diagnostic for amyloid-related disease
US20060057671A1 (en) * 2004-09-10 2006-03-16 Orser Cindy S Immobilized probes and methods of detecting conformationally altered prion proteins
NZ560535A (en) * 2005-01-13 2011-01-28 Novartis Vaccines & Diagnostic Elisa assays using prion-specific peptide reagents
WO2006076683A2 (en) * 2005-01-13 2006-07-20 Novartis Vaccines And Diagnostics Inc. Isolation and detection of pathogenic prions
ATE528649T1 (en) 2005-02-15 2011-10-15 Adlyfe Inc METHOD FOR DETECTING MISFOLDED PROTEINS AND PRIONS
WO2007008072A2 (en) 2005-07-13 2007-01-18 Crossbeta Biosciences B.V. CROSS-ß STRUCTURE BINDING COMPOUNDS
US20070015133A1 (en) * 2005-07-13 2007-01-18 Umc Utrecht Holding B.V. Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein
US8114832B2 (en) 2005-07-13 2012-02-14 Crossbeta Biosciences B.V. Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition
EP1931695B1 (en) 2005-09-09 2013-04-10 Novartis AG Prion-specific peptoid reagents
US20070224593A1 (en) * 2005-10-18 2007-09-27 Goldstein Lee E Diagnosis of transmissible spongiform encephalopathy
AU2007241729A1 (en) * 2006-04-21 2007-11-01 Peoplebio, Inc. Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides through three-dimensional interactions
US8673579B2 (en) * 2006-07-28 2014-03-18 Adlyfe, Inc. Peptide probes for diagnostics and therapeutics
WO2008029965A1 (en) * 2006-09-08 2008-03-13 Peoplebio, Inc. Simultaneous reaction assay for differentially detecting multimeric form
WO2009128948A1 (en) * 2008-04-17 2009-10-22 Peptimmune, Inc. Design and synthesis of directed sequence polymer compositions and antibodies thereof for the treatment of protein conformational disorders
US20110189692A1 (en) * 2008-04-30 2011-08-04 Novartis Ag Assay for pathogenic conformers
CN107677829A (en) * 2008-10-31 2018-02-09 耶鲁大学 Preeclampsia detects and the method and composition for the treatment of
MX2011007916A (en) 2009-01-30 2011-11-18 Adlyfe Inc Conformationally dynamic peptides.
WO2011149917A1 (en) 2010-05-25 2011-12-01 Adlyfe, Inc. STABILIZED AMYLOID- β OLIGOMERS AND USES THEREOF
AU2012249694A1 (en) 2011-04-27 2013-11-07 Adlyfe, Inc. Ocular detection of amyloid proteins
CN104780870B (en) 2012-09-25 2018-03-02 4网络公司 Programmable implant and the method for repairing bone structure using programmable implant
US9588129B2 (en) 2013-03-15 2017-03-07 Amira Medical Technologies Inc. Methods for analyzing blood to detect diseases associated with abnormal protein aggregation
CA3051839A1 (en) 2017-02-17 2018-08-23 Bristol-Myers Squibb Company Antibodies to alpha-synuclein and uses thereof
TWI698641B (en) * 2017-12-28 2020-07-11 大陸商浙江數問生物技術有限公司 Device, kit and method for detecting misfolded protein

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007473A1 (en) * 1999-07-28 2001-02-01 Kelvin Stott Peptides containing n-substituted l-amino acids for preventing beta-strand association
WO2001014412A1 (en) * 1999-08-23 2001-03-01 The Regents Of The University Of California Compounds useful to mimic peptide beta-strands
US20010001061A1 (en) * 1997-02-21 2001-05-10 Prusiner Stanley B. Assay for disease related conformation of a protein

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1001061A (en) * 1907-03-12 1911-08-22 Joseph Mcc Michaelson Logarithmic scale.
US4293221A (en) * 1979-04-17 1981-10-06 Research Corporation Multidimensional slit-scan flow system
US4444879A (en) 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5434050A (en) 1991-08-13 1995-07-18 Regents Of The University Of Minnesota Labelled β-amyloid peptide and methods of screening for Alzheimer's disease
DE69228701T2 (en) 1991-12-03 1999-07-29 Proteus Molecular Design Fragments of prion proteins.
US5955343A (en) 1992-12-28 1999-09-21 Massachusetts Institute Of Technology Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor
US5565186A (en) 1994-05-13 1996-10-15 The Regents Of The University Of California Method of detecting prions in a sample and transgenic animal used for same
ES2175083T3 (en) 1995-03-14 2002-11-16 Praecis Pharm Inc AMULOID AGGREGATION MODULATORS.
US5948763A (en) * 1995-06-07 1999-09-07 New York University Peptides and pharmaceutical compositions thereof for treatment of disorders or diseases associated with abnormal protein folding into amyloid or amyloid-like deposits
ATE263374T1 (en) 1995-09-14 2004-04-15 Univ California ANTIBODIES SPECIFIC TO NATIVE PRP-SC
US5750361A (en) 1995-11-02 1998-05-12 The Regents Of The University Of California Formation and use of prion protein (PRP) complexes
ATE249046T1 (en) 1996-05-14 2003-09-15 Winnacker Ernst Ludwig Prof CHAPERONES THAT BIND PRION PROTEINS AND CAN DIFFERENTIATE BETWEEN THE ISOFORMS PRPC AND PRPSC
US20060178302A1 (en) 1997-02-05 2006-08-10 Northwestern University & The University Of Southern California Amyloid beta protein (globular assembly and uses thereof)
US5891641A (en) 1997-02-21 1999-04-06 The Regents Of The University Of California Assay for disease related conformation of a protein
JP2001515044A (en) 1997-08-14 2001-09-18 ザ・レジェンツ・オブ・ザ・ユニバーシティー・オブ・カリフォルニア Fluorescent amyloid Aβ peptide and uses thereof
ATE223058T1 (en) 1997-09-19 2002-09-15 Evotec Ag METHOD FOR MEASURING THE ASSOCIATION OF PARTIAL STRUCTURES OF PATHOLOGICAL PROTEIN DEPOSITS
AU2600499A (en) 1998-02-13 1999-08-30 Arch Development Corporation Methods and compositions comprising the use of blocked b-amyloid peptide
US5977324A (en) * 1998-02-20 1999-11-02 The Regents Of The University Of California Process for concentrating protein with disease-related conformation
US6214565B1 (en) 1998-10-09 2001-04-10 The Regents Of The University Of California Assay for disease related conformation of a protein and isolating same
US6750025B1 (en) 1998-07-09 2004-06-15 V.I. Technologies, Inc. Method of detecting and isolating prion protein and variants thereof
US6186659B1 (en) * 1998-08-21 2001-02-13 Agilent Technologies Inc. Apparatus and method for mixing a film of fluid
GB2348203B (en) 1998-11-04 2002-06-19 Imp College Innovations Ltd Solube beta-forms of prion proteins, methods of preparation and use
US20050112607A1 (en) * 1999-01-23 2005-05-26 Bamdad Cynthia C. Rapid and sensitive detection of protein aggregation
CA2361013A1 (en) 1999-01-25 2000-07-27 Minerva Biotechnologies Corporation Rapid and sensitive detection of aberrant protein aggregation in neurodegenerative diseases
US6166187A (en) 1999-03-05 2000-12-26 The Regents Of The University Of California Method of concentrating prion proteins in blood samples
ES2257298T3 (en) 1999-05-17 2006-08-01 Conjuchem, Inc. PROTECTION OF ENDOGENOUS THERAPEUTIC PEPTIDES AGAINST THE ACTIVITY OF PEPTIDASE THROUGH CONJUGATION WITH BLOOD COMPONENTS.
AU780414B2 (en) 1999-07-27 2005-03-17 D-Gen Limited Fragments of cellular prion protein and methods useful in the diagnosis and treatment of prion diseases
US6399314B1 (en) * 1999-12-29 2002-06-04 American Cyanamid Company Methods of detection of amyloidogenic proteins
JP2003530554A (en) 2000-04-05 2003-10-14 ブイ．アイ．テクノロジーズ，インコーポレイテッド Prion binding peptide ligands and methods of using the same
AU6408901A (en) * 2000-07-07 2002-01-21 Applied Research Systems Early diagnosis of conformational diseases
US6780641B2 (en) 2000-07-10 2004-08-24 University Of British Columbia Immortalized human microglia cell line
US6495335B2 (en) 2000-12-07 2002-12-17 Mario Chojkier Compositions and methods for diagnosing alzheimer's disease
EP1577382A3 (en) 2001-01-08 2006-08-30 Health Protection Agency Detection of TSE infectivity
EP1448062A1 (en) 2001-04-17 2004-08-25 ISTA S.p.A. Detection and quantification of prion isoforms in neurodegenerative diseases using mass spectrometry
AU2002314794A1 (en) 2001-05-23 2002-12-03 New York University Detection of alzheimer's amyloid by magnetic resonance imaging
JP4235544B2 (en) 2001-05-31 2009-03-11 アドライフ インコーポレーティッド Defect folding protein sensor method
US20050026165A1 (en) 2001-05-31 2005-02-03 Cindy Orser Detection of conformationally altered proteins and prions
CA2451795A1 (en) 2001-06-26 2003-01-09 Paul M. Mathews Cell-based high-throughput screening methods
KR20030029251A (en) 2001-10-05 2003-04-14 삼성전자주식회사 Liquid crystal display device
CA2467914A1 (en) 2001-12-10 2003-06-19 Applied Research Systems Ars Holding N.V. Prion inhibiting peptides and derivatives thereof
US20040052928A1 (en) 2002-09-06 2004-03-18 Ehud Gazit Peptides and methods using same for diagnosing and treating amyloid-associated diseases
NZ535276A (en) 2002-02-28 2008-04-30 Microsens Biophage Ltd Binding of pathological forms of prion proteins
ES2380925T3 (en) 2002-04-09 2012-05-21 The Scripps Research Institute Hybrid polypeptides with grafted motifs and uses thereof
JP2004155688A (en) 2002-04-30 2004-06-03 Biofrontier Kenkyusho:Kk Synthetic peptide having chaperone activity, method for measuring decarbonation activity, medicine for transmissible spongiform encephalopathy, and its searching method
US20060057636A1 (en) 2002-08-21 2006-03-16 Peter Heegaard Composite peptide compounds for diagnosis and treatment of diseases caused by prion proteins
US20040072236A1 (en) 2002-09-27 2004-04-15 Neil Cashman PrPSc -interacting molecules and uses thereof
DK1953551T3 (en) 2002-12-03 2014-05-12 Univ North Carolina State Prion protein ligands and methods for their use
US7510848B2 (en) 2003-04-04 2009-03-31 North Carolina State University Prion protein binding materials and methods of use
US20070054322A1 (en) 2003-07-31 2007-03-08 Hadasit Medical Research Services & Development Lt Methods and kits for the detection of prion diseases
BRPI0413495A (en) 2003-08-13 2006-10-17 Chiron Corp prion-specific peptide reagents and their uses
US20060035242A1 (en) 2004-08-13 2006-02-16 Michelitsch Melissa D Prion-specific peptide reagents
JP3910569B2 (en) 2003-08-19 2007-04-25 独立行政法人科学技術振興機構 Reagent for amplifying amyloid fibrillation of amyloid β protein
US20060057671A1 (en) 2004-09-10 2006-03-16 Orser Cindy S Immobilized probes and methods of detecting conformationally altered prion proteins
ATE528649T1 (en) 2005-02-15 2011-10-15 Adlyfe Inc METHOD FOR DETECTING MISFOLDED PROTEINS AND PRIONS
US8673579B2 (en) 2006-07-28 2014-03-18 Adlyfe, Inc. Peptide probes for diagnostics and therapeutics
WO2009117042A1 (en) 2008-03-21 2009-09-24 Adlyfe, Inc. Use of pyrene to carry non-peptide agents across the blood brain barrier
CN102014967A (en) 2008-03-21 2011-04-13 阿德利夫股份有限公司 Use of pyrene to carry peptides across the blood brain barrier

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010001061A1 (en) * 1997-02-21 2001-05-10 Prusiner Stanley B. Assay for disease related conformation of a protein
WO2001007473A1 (en) * 1999-07-28 2001-02-01 Kelvin Stott Peptides containing n-substituted l-amino acids for preventing beta-strand association
WO2001014412A1 (en) * 1999-08-23 2001-03-01 The Regents Of The University Of California Compounds useful to mimic peptide beta-strands

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHITNUMSUB P ET AL: "THE NUCLEATION OF MONOMERIC PARALLEL BETA-SHEET-LIKE STRUCTURES AND THEIR SELF-ASSEMBLY IN AQUEOUS SOLUTION", BIOORGANIC & MEDICINAL CHEMISTRY, ELSEVIER SCIENCE LTD, GB, vol. 7, no. 1, January 1999 (1999-01-01), pages 39 - 59, XP002152178, ISSN: 0968-0896 *
FRASER P E ET AL: "Conformation and fibrillogenesis of Alzheimer A-beta peptides with selected substitution of charged residues", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 244, no. 1, 1994, pages 64 - 73, XP002957211, ISSN: 0022-2836 *
WILSON D ET AL: "CONFORMATIONAL TRANSITIONS IN MODEL SILK PEPTIDES", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 78, no. 5, May 2000 (2000-05-01), pages 2690 - 2701, XP002941678, ISSN: 0006-3495 *

Also Published As

Publication number Publication date
US8062895B2 (en) 2011-11-22
CA2448981A1 (en) 2002-12-05
EP1395833B1 (en) 2013-02-27
US7691639B2 (en) 2010-04-06
MXPA03011000A (en) 2004-02-27
WO2002097444A2 (en) 2002-12-05
US20030104633A1 (en) 2003-06-05
US20060286672A1 (en) 2006-12-21
JP4235544B2 (en) 2009-03-11
US20060275910A1 (en) 2006-12-07
AU2008203542B2 (en) 2011-01-20
AU2008203542A1 (en) 2008-08-28
CA2448981C (en) 2014-03-18
AU2002320045B2 (en) 2008-05-08
JP2004536296A (en) 2004-12-02
EP1395833A2 (en) 2004-03-10
US7166471B2 (en) 2007-01-23
US20100267151A1 (en) 2010-10-21

Similar Documents

Publication Publication Date Title
WO2002097444A3 (en) Misfolded protein sensor method
Bayramoglu et al. Design of an aptamer-based magnetic adsorbent and biosensor systems for selective and sensitive separation and detection of thrombin
ATE386936T1 (en) SELF-CALIBRATION SYSTEM FOR MAGNETIC BINDING PROCESS
US7759074B2 (en) Immunological latex turbidimetry method and reagent therefor
WO2003043487A3 (en) Method of using a non-antibody protein to detect and measure an analyte
WO2005066612A3 (en) Detection of biomolecules using porous biosensors and raman spectroscopy
SG169395A1 (en) Detection of cancer by elevated levels of bcl-2
Renny et al. Enzyme based sensor for detection of urea in milk
EP1700912A4 (en) Method of detecting target molecule by using aptamer
WO2004083859A8 (en) Agglutination based sample testing device
ATE515711T1 (en) WATER RELAXATION BASED SENSORS
WO2004046194A3 (en) Polyclonal-monoclonal elisa assay for detecting n-terminus probnp
AU6279399A (en) Methods and materials for detection and measurement of free radical damage
WO2001090749A3 (en) Scanning kelvin microprobe system and process for biomolecule microassay
CA2642648C (en) Detection of anthrax pathogenicity factors
Li et al. Target-induced structure switching of aptamers facilitates strand displacement for DNAzyme recycling amplification detection of thrombin in human serum
WO2006004958A3 (en) Detection or measurement of antibodies to antigenic proteins in biological tissues or samples
EP2251356A3 (en) Polyclonal-monoclonal ELISA assay for detecting N-terminus proBNP
Ricciardi et al. Immunodetection of 17β-estradiol in serum at ppt level by microcantilever resonators
Dukhanina et al. Spectral studies on the calcium-binding properties of Mts1 protein and its interaction with target protein
Liljeblad et al. Analysis of agalacto-IgG in rheumatoid arthritis using surface plasmon resonance
AU2002229974A1 (en) Biochemical method and apparatus for detecting protein characteristics
CA2366651A1 (en) A method for detecting megsin protein and use thereof
Temporini et al. On‐line multi‐enzymatic approach for improved sequence coverage in protein analysis
WO2006092669A3 (en) Methods of detecting a phenotype of a polymorphic protein

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002320045

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: PA/a/2003/011000

Country of ref document: MX

Ref document number: 2003500572

Country of ref document: JP

Ref document number: 2448981

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2002749544

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2002749544

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 2006286672

Country of ref document: US

Ref document number: 10494906

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 10494906

Country of ref document: US