WO2002083868A3 - Peroxide-driven cytochrome p450 oxygenase variants - Google Patents

Peroxide-driven cytochrome p450 oxygenase variants Download PDF

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Publication number
WO2002083868A3
WO2002083868A3 PCT/US2002/011954 US0211954W WO02083868A3 WO 2002083868 A3 WO2002083868 A3 WO 2002083868A3 US 0211954 W US0211954 W US 0211954W WO 02083868 A3 WO02083868 A3 WO 02083868A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytochrome
variants
peroxide
driven
compared
Prior art date
Application number
PCT/US2002/011954
Other languages
French (fr)
Other versions
WO2002083868A2 (en
Inventor
Patrick C Cirino
Frances H Arnold
Original Assignee
California Inst Of Techn
Patrick C Cirino
Frances H Arnold
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Inst Of Techn, Patrick C Cirino, Frances H Arnold filed Critical California Inst Of Techn
Priority to AU2002307340A priority Critical patent/AU2002307340A1/en
Priority to EP02762117A priority patent/EP1470219A4/en
Publication of WO2002083868A2 publication Critical patent/WO2002083868A2/en
Publication of WO2002083868A3 publication Critical patent/WO2002083868A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001Unspecific monooxygenase (1.14.14.1)

Abstract

The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have a improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T. L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
PCT/US2002/011954 2001-04-16 2002-04-16 Peroxide-driven cytochrome p450 oxygenase variants WO2002083868A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002307340A AU2002307340A1 (en) 2001-04-16 2002-04-16 Peroxide-driven cytochrome p450 oxygenase variants
EP02762117A EP1470219A4 (en) 2001-04-16 2002-04-16 Peroxide-driven cytochrome p450 oxygenase variants

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US28421501P 2001-04-16 2001-04-16
US60/284,215 2001-04-16
US28863601P 2001-05-03 2001-05-03
US60/288,636 2001-05-03
US34060201P 2001-12-12 2001-12-12
US60/340,602 2001-12-12

Publications (2)

Publication Number Publication Date
WO2002083868A2 WO2002083868A2 (en) 2002-10-24
WO2002083868A3 true WO2002083868A3 (en) 2004-08-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/011954 WO2002083868A2 (en) 2001-04-16 2002-04-16 Peroxide-driven cytochrome p450 oxygenase variants

Country Status (4)

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US (2) US7465567B2 (en)
EP (1) EP1470219A4 (en)
AU (1) AU2002307340A1 (en)
WO (1) WO2002083868A2 (en)

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Also Published As

Publication number Publication date
AU2002307340A1 (en) 2002-10-28
US20050202419A1 (en) 2005-09-15
EP1470219A4 (en) 2005-10-05
EP1470219A2 (en) 2004-10-27
US7465567B2 (en) 2008-12-16
WO2002083868A2 (en) 2002-10-24
US20090142821A1 (en) 2009-06-04
US7704715B2 (en) 2010-04-27

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