WO2002083292A2 - Chemical libraries based on coded particles - Google Patents
Chemical libraries based on coded particles Download PDFInfo
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- WO2002083292A2 WO2002083292A2 PCT/GB2002/001788 GB0201788W WO02083292A2 WO 2002083292 A2 WO2002083292 A2 WO 2002083292A2 GB 0201788 W GB0201788 W GB 0201788W WO 02083292 A2 WO02083292 A2 WO 02083292A2
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- library
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- code
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- microparticle
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
- B01J2219/00461—Beads and reaction vessel together
- B01J2219/00463—Directed sorting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
- B01J2219/00468—Beads by manipulation of individual beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/005—Beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00502—Particles of irregular geometry
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/0054—Means for coding or tagging the apparatus or the reagents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/0054—Means for coding or tagging the apparatus or the reagents
- B01J2219/00547—Bar codes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/0054—Means for coding or tagging the apparatus or the reagents
- B01J2219/00554—Physical means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/0059—Sequential processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00592—Split-and-pool, mix-and-divide processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00689—Automatic using computers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
Definitions
- the present invention relates to a method of fabricating chemical libraries on coded particles.
- oligomers formed from monomer units of a similar type to one another but differing in detailed structure .
- the aim may be to discover with which sequence of monomer units forming an oligomer a particular chemical entity will react. In some cases this may be for identifying the sequence of an unknown oligomer.
- the oligomers may be formed from nucleotides and their analogues (e.g. RNA, DNA or PNA) , amino acids (peptides and proteins), sugars or any other oligomerisable chemical compound.
- oligonucleotides With regard to oligonucleotides, one approach is to place an unknown (analyte) strand in the presence of all possible (target) strands, typically of some shorter length. A small number of complementary target strands will bind to the analyte strand and this binding event may be identified by any suitable means, for example fluorescence, electro- chemiluminescence, che iluminescence, biochemiluminescence or phosphorescence. In an existing technology, the target strands are spatially distributed on a surface. The sequence of each target strand is encoded in its location on the surface. The identity of the analyte strand can, therefore, be deduced from the physical location of the binding event in relation to the surface.
- peptide sequences are built-up on particles (one sequence per particle) and interaction between each peptide sequence and an active molecule such as a peptide cleaving enzyme is looked for.
- the substrate sequence for the enzyme may be discovered and inhibitors for it may be developed.
- the beads upon which a binding event occurred may then be separated from the bulk of the beads and the sequence of the analyte strand deduced from the sequence of the bound target strand that is identifiable by a number of means .
- One approach to placing the members of the library on the coded particles is to have the particles fixed on a substrate and to synthesise or place the library compounds on the particles in a spatially directed manner. Knowledge of the location at which each coded particle is present combined with knowledge of the location at which each library member was synthesised or placed provides knowledge of which particle code is associated with which library member. Each library of coded particles has to be created separately. Each synthesis step is effectively conducted separately for each bead.
- An alternative approach to building the required library is disclosed in W096/36436.
- coded particles linked to a memory device are provided with individual identifier codes. They are mixed together and split into a number of separate reaction containers based on the number of choices for the first component of the molecules of the library, e.g. four reaction containers each for one DNA nucleotide reactant .
- the codes of each particle going into each container are read and recorded. After synthesis of the first component, they are re-mixed and then split again into the reaction containers with the codes being read and recorded again.
- each particle is given a multitude of identical molecules of the library and all the desired library members are present on at least one particle. This process is referred to as
- a process of this type is also used in WO 97/15390 to build a library on physically encoded silicon microparticles in such a way that there is only one microparticle per library member.
- the code of each particle has to be read in each iteration. So, to make a single library containing say 10 10 compounds of say 10 sub-units in length, one has to read 10 10 particles on each of at least 10 occasions. To make a second library which is the same as the first one, one has to go through the whole procedure again.
- the present invention now provides a chemical library (e.g.
- a combinatorial chemical library comprising a plurality of holders, each holder having a distinguishing machine readable code (the holder code) and each holding a multiplicity of microparticles, each microparticle of each individual holder bearing a machine readable code (the microparticle code) and bearing a chemical or combination of chemicals within the library which is the same as a chemical or combination of chemicals borne by each other microparticle of the respective individual holder, such that each microparticle code maps to a unique chemical or combination of chemicals within the library and each holder code maps to a unique chemical or combination of chemicals within the library.
- Such a library may be prepared by taking a plurality of holders, each holder having a distinguishing machine readable code (the holder code) and each holding a multiplicity of microparticles, each microparticle of each individual holder bearing a machine readable code (the microparticle code) , in a first round of library synthesis splitting the plurality of holders into at least two groups (group la and group Ila and optionally N-2 further groups Ilia to Na) and conducting a first chemical library synthesis iteration on each group to add to each microparticle of each group of holders a first respective chemical library member component, in a second round of library synthesis re-splitting the plurality of holders into at least two new groups (group lb and group lib and optionally N-2 further groups Illb to Nb) and conducting a second chemical library synthesis iteration on each group to add to each microparticle of each group of holders a second respective chemical library member component, and in further rounds of library synthesis repeating said mixing, re-splitting and
- each holder may hold a multiplicity of coded microparticles
- the library produced can be further processed by removing the microparticles from the holders in such a way as either to produce multiple identical libraries or to produce one or more libraries in which each library member is present on a multiplicity of preferably identical microparticles .
- each microparticle code of each microparticle bearing a particular library member may be the same, but this is not essential. Accordingly, whilst each microparticle code maps to a single library member, it is not necessary that each library member maps to a single microparticle code.
- any chemical library according to the invention is associated with a database which contains for each microparticle code the identity of the chemical or chemicals mapped thereto.
- Said database may further contain for each holder code the identity of the chemical or chemicals mapped thereto.
- each chemical or combination of chemicals on a microparticle within the library maps to a unique holder code.
- more than one holder may hold microparticles having a particular library member thereon, and each said holder may if desired be differently encoded.
- each chemical or combination of chemicals on a microparticle within the library maps to a unique micro-particle code. This will imply that each microparticle associated with a particular holder has the same micro-particle code.
- microparticles associated with a single holder may have different micro-particle codes.
- the microparticle code may be provided by the shape of each microparticle.
- GB 0009723 describes particles formed in plastics. Each generally rod shaped particle has a series of notches along each long side which form a machine readable bar code. Optionally, one end of each particle is marked with a notch serving to differentiate that end from the other end of the particle.
- Microparticles of this type are preferred for use according to the invention. However, other shaped microparticles may be used including those described in WO97/15390, which describes shaped microparticles made in silicon and encoded by pits, holes, hollows, grooves, or notches or combinations thereof. Any such morphological features may be used according to the present invention. However, the microparticle code does not need to be produced by shaping the microparticles.
- microparticle code may be a bar code printed on the particle.
- each microparticle has a maximum dimension of less than 5mm, preferably less than 1mm, more preferably less than 500 ⁇ m, e.g. about 250 ⁇ m.
- the beads may be of any shape, e.g. shaped as cubes, spheres, rods, plates, discs or blocks. Preferably, they are generally rod shaped and have a lesser dimension of from 10 to 100 ⁇ m, e.g. about 25 ⁇ m.
- the holder code may be provided by any of the methods described above.
- the holder may be a container in which the micro-particles are free. Such a container will need to allow reagents to pass in and out whilst the microparticles are retained.
- the container has porous walls. It may be in the form of a bag of flexible material but is more preferably of rigid construction, having one or more walls of mesh or other permeable material .
- each said oligomer is an oligonucleic acid, an oligonucleic acid analogue, a peptide, a peptide containing one or more non-peptide linking groups, or a sugar.
- Oligonucleic acids will include DNA and RNA oligomers, preferably of from 5 to 20, e.g. from 8 to 14 nucleotides in length.
- Nucleic acid analogues are compounds which are different from DNA or RNA but which mimic the ability of DNA or RNA to hybridise to DNA or RNA strands of complementary sequence. These analogues typically have a modified backbone designed to resist proteolytic degradation by nucleases . This may be for instance a peptide backbone as in PNAs or hybrid PNA-DNAs.
- the invention includes a method of forming a combinatorial chemical library, which method comprises taking a plurality of holders, each holder having a distinguishing machine readable code (the holder code) and each holding a multiplicity of microparticles, each microparticle of each individual holder bearing a machine readable code (the microparticle code) , in a first round of library synthesis splitting the plurality of holders into at least two groups (group la and group Ila and optionally N-2 further groups Ilia to Na) and conducting a first chemical library synthesis iteration on each group to add to each micro- particle of each group of holders a first respective chemical library member component, in a second round of library synthesis re-splitting the plurality of holders into at least two new groups (group lb and group lib and optionally N-2 further groups Illb to Nb) and conducting a second chemical library synthesis iteration on each group to add to each microparticle of each group of holders a second respective chemical library member component, and in further rounds of library
- the method may further comprise removing the microparticles from said holders.
- all of the holders may be returned to the master storage chamber prior to re-splitting into new groups.
- the invention includes a support unit for use in synthesising a chemical library comprising a holder and a multitude of microparticles held by the holder, each microparticle being encoded with an identical code and each being suitable as a substrate on which to synthesise a chemical library, the holder being such as to provide access to the microparticles for reagents applied to the holder during library synthesis.
- a support unit for use in synthesising a chemical library comprising a holder and a multitude of microparticles held by the holder, each microparticle being encoded with an identical code and each being suitable as a substrate on which to synthesise a chemical library, the holder being such as to provide access to the microparticles for reagents applied to the holder during library synthesis.
- the invention further includes apparatus for use in synthesising a chemical library comprising a conduit connecting a master storage chamber to a plurality of reaction chambers branching off said conduit at respective branch points, at or before each branch point a code reader suitable for reading codes present on microparticle holders passing along said conduit in use and a valve associated with each reaction chamber and operable in response to the reading of codes by a respective said code reader to open temporarily to allow a selected said holder to pass into the respective reaction chamber.
- Figure 1 shows in side view a holder for use in the invention
- Figure 2 shows apparatus for use in synthesising a combinatorial chemical library.
- Figure 1 shows a holder for use in the method of library synthesis of the invention. It comprises a small pot 2 having a solid base and rigid mesh sides 4 and a removable lid 6. Each holder is marked with a machine readable bar code. However, other methods of coding and the holders may be employed.
- the lid may contain a microchip containing a remotely readable code uniquely identifying the holder.
- Within the holder there are contained a multitude of microparticles each of which is shaped by a series of bars and grooves along at least one side which constitute a bar code. Each microparticle within the holder has the same bar code.
- the code on the microparticles within each holder will be the same and will differ from the code of the microparticles in each other holder.
- microparticles may be subjected to chemical combinatorial library synthesis using conventional and accepted chemical methodology in the apparatus shown in
- a master chamber 10 is connected to one end of a conduit 12. From conduit 12 there are four branch conduits 14, 16, 18, 20 which join the conduit 12 at respective branch points. Each branch conduit leads to a respective reaction chamber 11, 13, 15, 17. At each branch point a respective holder code reader (not shown) is provided at the locations 22, 24, 26, 28. Each reader is designed to read the code associated with a holder of the kind shown in Figure 1 as such a holder passes down the conduit 12. A valve (not shown) is provided at each branch point . Each code reader is adapted to communicate with the adjacent valve via a computer (not shown) to cause the valve to open when a desired code is read.
- each microparticle within each holder will bear numerous molecules of a particular library member. In any single holder each microparticle will bear the same library member. At this point one may either produce multiple identical libraries by opening the holders and allocating one or more of the microbeads from each holder to a respective library or one may produce a library in which each library member is represented by numerous microparticles simply by opening each holder and mixing all of the contents together.
- Methods of combinatorial chemical synthesis suitable for use in the context of this invention include those already known generally in the art and do not require detailed description here. Examples of suitable synthesis techniques are for instance given in WO 96/36436 and WO 96/24061. Code readers of the kind described in those specifications may be employed in this invention also.
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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GB0109545.4 | 2001-04-18 | ||
GB0109545A GB0109545D0 (en) | 2001-04-18 | 2001-04-18 | Chemical libraries based on coded particles |
Publications (2)
Publication Number | Publication Date |
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WO2002083292A2 true WO2002083292A2 (en) | 2002-10-24 |
WO2002083292A3 WO2002083292A3 (en) | 2003-01-16 |
Family
ID=9913003
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/GB2002/001788 WO2002083292A2 (en) | 2001-04-18 | 2002-04-18 | Chemical libraries based on coded particles |
Country Status (2)
Country | Link |
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GB (1) | GB0109545D0 (en) |
WO (1) | WO2002083292A2 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7745091B2 (en) | 2005-09-13 | 2010-06-29 | Affymetrix, Inc. | Miniaturized microparticles |
US8119408B2 (en) * | 2004-03-31 | 2012-02-21 | Kabushiki Kaisha Toshiba | Encoded carrier and a method of monitoring an encoded carrier |
US8178278B2 (en) | 2005-09-13 | 2012-05-15 | Affymetrix, Inc. | Miniaturized microparticles |
US10481158B2 (en) | 2015-06-01 | 2019-11-19 | California Institute Of Technology | Compositions and methods for screening T cells with antigens for specific populations |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1997015390A1 (en) * | 1995-10-26 | 1997-05-01 | University Of Hertfordshire | Coded particles for process sequence tracking in combinatorial compound library preparation |
WO1999055456A1 (en) * | 1998-04-25 | 1999-11-04 | Central Research Laboratories Limited | Method of synthesizing a plurality of products |
US6136274A (en) * | 1996-10-07 | 2000-10-24 | Irori | Matrices with memories in automated drug discovery and units therefor |
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2001
- 2001-04-18 GB GB0109545A patent/GB0109545D0/en not_active Ceased
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2002
- 2002-04-18 WO PCT/GB2002/001788 patent/WO2002083292A2/en not_active Application Discontinuation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997015390A1 (en) * | 1995-10-26 | 1997-05-01 | University Of Hertfordshire | Coded particles for process sequence tracking in combinatorial compound library preparation |
US6136274A (en) * | 1996-10-07 | 2000-10-24 | Irori | Matrices with memories in automated drug discovery and units therefor |
WO1999055456A1 (en) * | 1998-04-25 | 1999-11-04 | Central Research Laboratories Limited | Method of synthesizing a plurality of products |
Non-Patent Citations (3)
Title |
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G]NTHER JUNG & ANNETTE G. BECK-SICKINGER: "MULTIPLE PEPTIDE SYNTHESIS METHODS AND THEIR APPLICATIONS" ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VERLAG CHEMIE, vol. 31, no. 4, 1 April 1992 (1992-04-01), pages 367-383, XP000325208 WEINHEIM, DE ISSN: 0570-0833 * |
JOSEPH W. GUILES ET AL.: "A Visual Tagging Process for Mix and Sort Combinatorial Chemistry" ANGEWANDTE CHEMIE. INTERNATIONAL EDITION., vol. 37, no. 7, 1998, pages 926-928, XP002220516 VERLAG CHEMIE. WEINHEIM., DE ISSN: 0570-0833 * |
ROBERT W. ARMSTRONG ET AL.: "MICROCHIP ENCODED COMBINATORIAL LIBRARIES: GENERATION OF A SPATIALLY ENCODED LIBRARY FROM A POOL SYNTHESIS" CHIMIA, vol. 50, no. 6, 1 June 1996 (1996-06-01), pages 258-260, XP000617072 AARAU, CH ISSN: 0009-4293 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8119408B2 (en) * | 2004-03-31 | 2012-02-21 | Kabushiki Kaisha Toshiba | Encoded carrier and a method of monitoring an encoded carrier |
US7745091B2 (en) | 2005-09-13 | 2010-06-29 | Affymetrix, Inc. | Miniaturized microparticles |
US7745092B2 (en) | 2005-09-13 | 2010-06-29 | Affymetrix, Inc. | Multiple step printing methods for microbarcodes |
US8088555B2 (en) | 2005-09-13 | 2012-01-03 | Affymetrix, Inc. | Multiple step printing methods for microbarcodes |
US8168368B2 (en) | 2005-09-13 | 2012-05-01 | Affymetrix, Inc. | Miniaturized microparticles |
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WO2002083292A3 (en) | 2003-01-16 |
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