WO2002064796A3 - Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use - Google Patents

Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use Download PDF

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Publication number
WO2002064796A3
WO2002064796A3 PCT/US2001/050450 US0150450W WO02064796A3 WO 2002064796 A3 WO2002064796 A3 WO 2002064796A3 US 0150450 W US0150450 W US 0150450W WO 02064796 A3 WO02064796 A3 WO 02064796A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
butyrylcholinesterase
polypeptide variants
catalytic efficiency
increased catalytic
Prior art date
Application number
PCT/US2001/050450
Other languages
French (fr)
Other versions
WO2002064796A2 (en
WO2002064796A8 (en
Inventor
Oksana Lockridge
Jeffry D Watkins
James D Pancook
Original Assignee
Applied Molecular Evolution
Univ Nebraska Medical Ct
Oksana Lockridge
Jeffry D Watkins
James D Pancook
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/748,739 external-priority patent/US7070973B2/en
Priority claimed from US10/032,233 external-priority patent/US20030153062A1/en
Application filed by Applied Molecular Evolution, Univ Nebraska Medical Ct, Oksana Lockridge, Jeffry D Watkins, James D Pancook filed Critical Applied Molecular Evolution
Priority to JP2002565109A priority Critical patent/JP4252311B2/en
Priority to CA002433057A priority patent/CA2433057A1/en
Priority to EP01997137A priority patent/EP1414974A2/en
Priority to AU2002248256A priority patent/AU2002248256B2/en
Publication of WO2002064796A2 publication Critical patent/WO2002064796A2/en
Publication of WO2002064796A8 publication Critical patent/WO2002064796A8/en
Publication of WO2002064796A3 publication Critical patent/WO2002064796A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The invention provides twenty-five butyrylcholinesterase variants having increased cocaine hydrolysis activity as well as the corresponding encoding nucleic acids. The invention also provides libraries of butyrylcholinesterase variants as well as libraries of the corresponding nucleic acids encoding butyrylcholinesterase variants. The invention further provides methods of hydrolyzing a cocaine-based butyrylcholinesterase substrate as well as methods of treating a cocaine-induced condition.
PCT/US2001/050450 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use WO2002064796A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2002565109A JP4252311B2 (en) 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use thereof
CA002433057A CA2433057A1 (en) 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use
EP01997137A EP1414974A2 (en) 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use
AU2002248256A AU2002248256B2 (en) 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/748,739 US7070973B2 (en) 2000-12-26 2000-12-26 Butyrylcholinesterase variants and methods of use
US09/748,739 2000-12-26
US10/032,233 2001-12-20
US10/032,233 US20030153062A1 (en) 2001-12-20 2001-12-20 Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use

Publications (3)

Publication Number Publication Date
WO2002064796A2 WO2002064796A2 (en) 2002-08-22
WO2002064796A8 WO2002064796A8 (en) 2003-11-13
WO2002064796A3 true WO2002064796A3 (en) 2003-12-31

Family

ID=26708157

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050450 WO2002064796A2 (en) 2000-12-26 2001-12-21 Butyrylcholinesterase polypeptide variants with increased catalytic efficiency and methods of use

Country Status (5)

Country Link
EP (1) EP1414974A2 (en)
JP (1) JP4252311B2 (en)
AU (1) AU2002248256B2 (en)
CA (1) CA2433057A1 (en)
WO (1) WO2002064796A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL142875A (en) * 2001-04-30 2009-08-03 Avigdor Shafferman Cholinesterases conjugated to peg for scavenging organophosphates in circulation
ATE361471T1 (en) 2001-12-05 2007-05-15 Sense Proteomic Ltd PROTEIN ARRAYS FOR ALLEL VARIANTS AND THEIR USE
US6989261B2 (en) * 2001-12-20 2006-01-24 Eli Lilly And Company Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use
US20080194481A1 (en) * 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
GB0202018D0 (en) 2002-01-29 2002-03-13 Sense Proteomic Ltd Tag and method
EP1581253A4 (en) * 2002-12-04 2007-02-14 Applied Molecular Evolution Butyrylcholinesterase variants that alter the activity of chemotherapeutic agents
CA2659097A1 (en) * 2006-06-07 2007-12-21 Human Biomolecular Research Institute Novel methods for ester detoxication
WO2015168207A1 (en) 2014-04-29 2015-11-05 Mayo Foundation For Medical Education And Research Butyrylcholinesterases having an enhanced ability to hydrolyze acyl ghrelin
WO2018053216A1 (en) 2016-09-15 2018-03-22 Mayo Foundation For Medical Education And Research Methods and materials for using butyrylcholinesterases to treat cancer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001625A (en) * 1995-05-19 1999-12-14 The United States Of America As Represented By The Secretary Of The Army Site-directed mutagenesis of esterases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001625A (en) * 1995-05-19 1999-12-14 The United States Of America As Represented By The Secretary Of The Army Site-directed mutagenesis of esterases

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ASHANI YACOV: "Prospective of human butyrylcholinesterase as a detoxifying antidote and potential regulator of controlled-release drugs.", DRUG DEVELOPMENT RESEARCH, vol. 50, no. 3-4, July 2000 (2000-07-01), pages 298 - 308, XP009008406, ISSN: 0272-4391 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 2000, SUN H ET AL: "Re-engineering butyrylcholinesterase as a cocaine hydrolase.", XP002236143, Database accession no. PREV200100102260 *
DATABASE EMBL [online] XP002236148, retrieved from EBI Database accession no. AR070208 *
DATABASE EMBL [online] XP002236149, retrieved from EBI Database accession no. M16541 *
DATABASE GENESEQN [online] XP002236147, retrieved from EBI Database accession no. AAZ49470 *
DATABASE GENESEQP [online] XP002236144, retrieved from EBI Database accession no. AAE40560 *
DATABASE GENESEQP [online] XP002236145, retrieved from EBI Database accession no. AAY44573 *
DATABASE GENESEQP [online] XP002236146, retrieved from EBI Database accession no. AAY59235 *
SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 26, no. 1-2, 2000, 30th Annual Meeting of the Society of Neuroscience;New Orleans, LA, USA; November 04-09, 2000, pages Abstract No. - 675.10, ISSN: 0190-5295 *
XIE WEIHUA ET AL: "An improved cocaine hydrolase: The A328Y mutant of human butyrylcholinesterase is 4-fold more efficient.", MOLECULAR PHARMACOLOGY, vol. 55, no. 1, January 1999 (1999-01-01), pages 83 - 91, XP002236142, ISSN: 0026-895X *

Also Published As

Publication number Publication date
AU2002248256B2 (en) 2006-09-28
EP1414974A2 (en) 2004-05-06
CA2433057A1 (en) 2002-08-22
JP4252311B2 (en) 2009-04-08
JP2004535155A (en) 2004-11-25
WO2002064796A2 (en) 2002-08-22
WO2002064796A8 (en) 2003-11-13

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