WO2002059353A3 - Two-step amplification using a program primer followed by specific primers - Google Patents

Two-step amplification using a program primer followed by specific primers Download PDF

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Publication number
WO2002059353A3
WO2002059353A3 PCT/CA2002/000057 CA0200057W WO02059353A3 WO 2002059353 A3 WO2002059353 A3 WO 2002059353A3 CA 0200057 W CA0200057 W CA 0200057W WO 02059353 A3 WO02059353 A3 WO 02059353A3
Authority
WO
WIPO (PCT)
Prior art keywords
amplification
dna
reaction
approach
sequencing
Prior art date
Application number
PCT/CA2002/000057
Other languages
French (fr)
Other versions
WO2002059353A2 (en
Inventor
Qinyin Cai
Peng Ling
Jin-Hao Liu
Original Assignee
Bio S & T
Qinyin Cai
Peng Ling
Jin-Hao Liu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002332610A external-priority patent/CA2332610A1/en
Application filed by Bio S & T, Qinyin Cai, Peng Ling, Jin-Hao Liu filed Critical Bio S & T
Priority to AU2002231489A priority Critical patent/AU2002231489A1/en
Publication of WO2002059353A2 publication Critical patent/WO2002059353A2/en
Publication of WO2002059353A3 publication Critical patent/WO2002059353A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A DNA amplification approach, enabling the amplification of DNA targets with unknown sequences, involves successive PCR steps. In a first reaction, linear amplification of the target nucleic acid is achieved using one or more specially designed primers. In the second reaction, additional primers are added and exponential amplification of a double stranded DNA is achieved. Combined with conventional DNA sequencing procedures, this approach can be used for the direct sequencing of genomic DNA, avoiding the need to sub-clone large fragments. With the inventive method, moreover, sequencing information can be gathered in a much less costly and labor-intensive manner than was possible previously.
PCT/CA2002/000057 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers WO2002059353A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002231489A AU2002231489A1 (en) 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,332,610 2001-01-26
CA002332610A CA2332610A1 (en) 2001-01-26 2001-01-26 Asymmetrical pcr applification
US09/825,873 US20020155448A1 (en) 2001-01-26 2001-04-05 Asymmetrical PCR amplification
US09/825,873 2001-04-05

Publications (2)

Publication Number Publication Date
WO2002059353A2 WO2002059353A2 (en) 2002-08-01
WO2002059353A3 true WO2002059353A3 (en) 2003-08-21

Family

ID=25682361

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/000057 WO2002059353A2 (en) 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers

Country Status (1)

Country Link
WO (1) WO2002059353A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10253337B4 (en) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Method for detecting a nucleic acid
WO2006094360A1 (en) * 2005-03-11 2006-09-14 Molecular Plant Breeding Nominees Ltd Method of amplifying nucleic acid
EP1947196A4 (en) * 2005-11-08 2009-09-09 Olympus Corp Method of amplifying multiple nucleic acid sequences for differentiation
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2008154467A (en) * 2006-12-21 2008-07-10 Olympus Corp Method for amplifying nucleic acid and method for analyzing nucleic acid using the same
CN102732609B (en) * 2011-04-08 2014-03-26 博奥生物有限公司 Method for detecting similarity of oligonucleotide and target genome
HU229967B1 (en) * 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Method for determining the sequence of fragmented nucleic acids
US9139870B2 (en) 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
CN106715714B (en) * 2014-10-17 2021-11-09 深圳华大智造科技股份有限公司 Primer for random fragmentation of nucleic acid and random fragmentation method of nucleic acid

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOOTH CLAIRE ET AL: "Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 21, 1 November 2001 (2001-11-01), pages 4414 - 4422, XP002242797, ISSN: 0305-1048 *
FRITZ J D ET AL: "A NOVEL 3' EXTENSION TECHNIQUE USING RANDOM PRIMERS IN RNA-PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 13, 11 July 1991 (1991-07-11), pages 3747, XP000371658, ISSN: 0305-1048 *
PODDAR SK: "Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 14, February 2000 (2000-02-01), pages 25 - 32, XP000953250, ISSN: 0890-8508 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 13, 1992, pages 718 - 725, XP000199116, ISSN: 0888-7543 *
WELSH J ET AL: "FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 24, 25 December 1990 (1990-12-25), pages 7213 - 7218, XP000310554, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
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