WO2001096857A3 - Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures - Google Patents

Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures Download PDF

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Publication number
WO2001096857A3
WO2001096857A3 PCT/US2001/019484 US0119484W WO0196857A3 WO 2001096857 A3 WO2001096857 A3 WO 2001096857A3 US 0119484 W US0119484 W US 0119484W WO 0196857 A3 WO0196857 A3 WO 0196857A3
Authority
WO
WIPO (PCT)
Prior art keywords
constituents
chamber
magnetophoretic
sample
combined
Prior art date
Application number
PCT/US2001/019484
Other languages
French (fr)
Other versions
WO2001096857A2 (en
Inventor
Peter R C Gascoyne
Frederick F Becker
Jody Vykoukal
Original Assignee
Univ Texas
Peter R C Gascoyne
Frederick F Becker
Jody Vykoukal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas, Peter R C Gascoyne, Frederick F Becker, Jody Vykoukal filed Critical Univ Texas
Priority to AU2001271330A priority Critical patent/AU2001271330A1/en
Priority to EP01950328.3A priority patent/EP1350095B1/en
Priority to CA002413634A priority patent/CA2413634A1/en
Priority to JP2002510936A priority patent/JP2004503775A/en
Publication of WO2001096857A2 publication Critical patent/WO2001096857A2/en
Publication of WO2001096857A3 publication Critical patent/WO2001096857A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/23—Magnetic separation acting directly on the substance being separated with material carried by oscillating fields; with material carried by travelling fields, e.g. generated by stationary magnetic coils; Eddy-current separators, e.g. sliding ramp
    • B03C1/24—Magnetic separation acting directly on the substance being separated with material carried by oscillating fields; with material carried by travelling fields, e.g. generated by stationary magnetic coils; Eddy-current separators, e.g. sliding ramp with material carried by travelling fields
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/0015—Field flow fractionation characterised by driving force
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005—Field flow fractionation
    • G01N2030/004—Field flow fractionation characterised by opposing force
    • G01N2030/0065—Dielectric FFF, i.e. opposing forces dominate hydrodynamic lift forces and steric effects

Abstract

Methods and apparatuses for discriminating matter utilizing dielectrophoresis combined with magnetophoresis. A sample having one or more constituents is injected into an inlet port of a chamber. A carrier medium flow is initiated at the inlet port to establish flow within the chamber. A dielectrophoretic force is generated to act on the constituents of the sample. A magnetophoretic force is generated to act on the constituents of the sample. The dielectrophoretic force and magnetophoretic forces are balanced to position the constituents within the chamber. The constituents are then collected at one or more outlet ports of the chamber according to the dielectric and magnetic characteristics of the constituents. The constituents may be collected as a function of time-of-exit from the chamber and/or position within the chamber.
PCT/US2001/019484 2000-06-14 2001-06-14 Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures WO2001096857A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001271330A AU2001271330A1 (en) 2000-06-14 2001-06-14 Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures
EP01950328.3A EP1350095B1 (en) 2000-06-14 2001-06-14 Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures
CA002413634A CA2413634A1 (en) 2000-06-14 2001-06-14 Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures
JP2002510936A JP2004503775A (en) 2000-06-14 2001-06-14 Method and apparatus for the operation of combined magnetophoresis and dielectrophoresis of analyte mixtures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21175700P 2000-06-14 2000-06-14
US60/211,757 2000-06-14

Publications (2)

Publication Number Publication Date
WO2001096857A2 WO2001096857A2 (en) 2001-12-20
WO2001096857A3 true WO2001096857A3 (en) 2003-07-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019484 WO2001096857A2 (en) 2000-06-14 2001-06-14 Method and apparatus for combined magnetophoretic and dielectrophoretic manipulation of analyte mixtures

Country Status (6)

Country Link
US (1) US7033473B2 (en)
EP (1) EP1350095B1 (en)
JP (1) JP2004503775A (en)
AU (1) AU2001271330A1 (en)
CA (1) CA2413634A1 (en)
WO (1) WO2001096857A2 (en)

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