WO2001084145A3 - Device for monitoring analytes at sub-micromolar concentrations - Google Patents

Device for monitoring analytes at sub-micromolar concentrations Download PDF

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Publication number
WO2001084145A3
WO2001084145A3 PCT/IB2001/000936 IB0100936W WO0184145A3 WO 2001084145 A3 WO2001084145 A3 WO 2001084145A3 IB 0100936 W IB0100936 W IB 0100936W WO 0184145 A3 WO0184145 A3 WO 0184145A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
nucleic acid
transcription regulatory
regulatory activity
sub
Prior art date
Application number
PCT/IB2001/000936
Other languages
French (fr)
Other versions
WO2001084145A2 (en
WO2001084145B1 (en
Inventor
Elisabeth Csoregi
Bo Mattiasson
Original Assignee
Forskarpatent I Syd Ab
Elisabeth Csoregi
Bo Mattiasson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forskarpatent I Syd Ab, Elisabeth Csoregi, Bo Mattiasson filed Critical Forskarpatent I Syd Ab
Priority to AU58692/01A priority Critical patent/AU5869201A/en
Publication of WO2001084145A2 publication Critical patent/WO2001084145A2/en
Publication of WO2001084145A3 publication Critical patent/WO2001084145A3/en
Publication of WO2001084145B1 publication Critical patent/WO2001084145B1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity

Abstract

The invention provides a device for detecting sub-micromolar concentrations of substances that interact with proteins having nucleic acid transcription regulatory activity. The device comprises an electrode having a surface bound by proteins having nucleic acid transcription regulatory activity. Interaction of the protein with a substance induces a conformational change in the protein, resulting in generation of a signal by the device. The invention also provides methods of detecting substances that interact with proteins having nucleic acid transcription regulatory activity as well as methods of identifying active sites of proteins having nucleic acid transcription regulatory activity.
PCT/IB2001/000936 2000-05-04 2001-05-04 Device for monitoring analytes at sub-micromolar concentrations WO2001084145A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU58692/01A AU5869201A (en) 2000-05-04 2001-05-04 Device for monitoring analytes at sub-micromolar concentrations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0001669-1 2000-05-04
SE0001669A SE0001669D0 (en) 2000-05-04 2000-05-04 Monitoring of analytes at submicromolar with extremely high sensitivity

Publications (3)

Publication Number Publication Date
WO2001084145A2 WO2001084145A2 (en) 2001-11-08
WO2001084145A3 true WO2001084145A3 (en) 2002-03-21
WO2001084145B1 WO2001084145B1 (en) 2002-07-04

Family

ID=20279567

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/000936 WO2001084145A2 (en) 2000-05-04 2001-05-04 Device for monitoring analytes at sub-micromolar concentrations

Country Status (3)

Country Link
AU (1) AU5869201A (en)
SE (1) SE0001669D0 (en)
WO (1) WO2001084145A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020155476A1 (en) 2000-10-20 2002-10-24 Nader Pourmand Transient electrical signal based methods and devices for characterizing molecular interaction and/or motion in a sample
AU2003239050A1 (en) * 2002-06-19 2004-01-06 Biosensor Applications Sweden Ab (Publ) Coated metal surface on solid support for displacement reactions

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
WO2000004390A2 (en) * 1998-07-14 2000-01-27 Zyomyx, Inc. Microdevices for screening biomolecules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
WO2000004390A2 (en) * 1998-07-14 2000-01-27 Zyomyx, Inc. Microdevices for screening biomolecules

Also Published As

Publication number Publication date
WO2001084145A2 (en) 2001-11-08
AU5869201A (en) 2001-11-12
WO2001084145B1 (en) 2002-07-04
SE0001669D0 (en) 2000-05-04

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