WO2001073000A3 - Methods for modulating cellular and organismal phenotypes - Google Patents

Methods for modulating cellular and organismal phenotypes Download PDF

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Publication number
WO2001073000A3
WO2001073000A3 PCT/US2001/009203 US0109203W WO0173000A3 WO 2001073000 A3 WO2001073000 A3 WO 2001073000A3 US 0109203 W US0109203 W US 0109203W WO 0173000 A3 WO0173000 A3 WO 0173000A3
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WO
WIPO (PCT)
Prior art keywords
cells
conjoint
concatamers
libraries
recombinant
Prior art date
Application number
PCT/US2001/009203
Other languages
French (fr)
Other versions
WO2001073000A2 (en
Inventor
Willem P C Stemmer
Jeremy Minshull
Robert J Keenan
Original Assignee
Maxygen Inc
Willem P C Stemmer
Jeremy Minshull
Robert J Keenan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Willem P C Stemmer, Jeremy Minshull, Robert J Keenan filed Critical Maxygen Inc
Priority to EP01962421A priority Critical patent/EP1276861A2/en
Priority to AU2001287273A priority patent/AU2001287273A1/en
Publication of WO2001073000A2 publication Critical patent/WO2001073000A2/en
Publication of WO2001073000A3 publication Critical patent/WO2001073000A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Abstract

Methods for identifying and controlling the genetic and metabolic pathways underlying complex phenotypes are provided. Conjoint polynucleotide segments that contribute to or disrupt elements of a multigenic phenotype are produced and expressed in cells of interest. Conjoint polynucleotide segments are recombined and/or mutated to give rise to libraries of recombinant concatamers which are expressed in cells of interest. Libraries of conjoint polynucleotide segments and recombinant concatamers are expressed episomally or integrated into the DNA of organelles or chromosomes. Cells are screened or selected to identify members of the population of cells exhibiting a desired phenotype. Libraries and vectors comprising conjoint polynucleotide segments and recombinant concatamers, as well as cells expressing such libraries and vectors or their components are provided. Kits containing conjoint polynucleotide segments, recombinant concatamers, vectors including such polynucleotides, and cells including such polynucleotides and vectors are provided.
PCT/US2001/009203 2000-03-24 2001-03-23 Methods for modulating cellular and organismal phenotypes WO2001073000A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01962421A EP1276861A2 (en) 2000-03-24 2001-03-23 Methods for modulating cellular and organismal phenotypes
AU2001287273A AU2001287273A1 (en) 2000-03-24 2001-03-23 Methods for modulating cellular and organismal phenotypes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US19178200P 2000-03-24 2000-03-24
US60/191,782 2000-03-24
US26261701P 2001-01-17 2001-01-17
US60/262,617 2001-01-17

Publications (2)

Publication Number Publication Date
WO2001073000A2 WO2001073000A2 (en) 2001-10-04
WO2001073000A3 true WO2001073000A3 (en) 2002-06-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009203 WO2001073000A2 (en) 2000-03-24 2001-03-23 Methods for modulating cellular and organismal phenotypes

Country Status (4)

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US (3) US20010049104A1 (en)
EP (1) EP1276861A2 (en)
AU (1) AU2001287273A1 (en)
WO (1) WO2001073000A2 (en)

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US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
DE60239386D1 (en) * 2001-01-25 2011-04-21 Evolva Ltd CELL LIBRARY
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
KR100385905B1 (en) * 2001-05-17 2003-06-02 주식회사 웰진 Method for high-throughput screening and functional analysis of genes using unigene antisense library
US20030054354A1 (en) * 2001-08-23 2003-03-20 Bennett C. Frank Use of antisense oligonucleotide libraries for identifying gene function
DE60324385D1 (en) * 2002-01-25 2008-12-11 Evolva Ag SCREENING METHODS FOR MULTIPLE PARAMETERS AND DEVELOPMENT OF CELLS TO PRODUCE SMALL MOLECULES WITH MULTIPLE FUNCTIONALITIES
EP1511843B1 (en) * 2002-06-07 2006-03-29 Sophion Bioscience A/S Screening methods
US20060252156A1 (en) * 2002-08-01 2006-11-09 Evolva Ltd. C/O Dr. Iur. Martin Eisenring Methods of mixing large numbers of heterologous genes
AU2012209017B2 (en) * 2005-02-03 2014-06-26 Antitope Limited Human antibodies and proteins
WO2006102540A2 (en) * 2005-03-24 2006-09-28 Syracuse University Elucidation of high affinity, high specificity oligonucleotides and derivatized oligonucleotide sequences for target recognition
WO2009021232A2 (en) * 2007-08-09 2009-02-12 Massachusetts Institute Of Technology High-throughput, whole-animal screening system
EP3690044B1 (en) * 2014-02-11 2024-01-10 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering

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Also Published As

Publication number Publication date
EP1276861A2 (en) 2003-01-22
WO2001073000A2 (en) 2001-10-04
US20040203046A1 (en) 2004-10-14
US20080287314A1 (en) 2008-11-20
US20010049104A1 (en) 2001-12-06
AU2001287273A1 (en) 2001-10-08

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