WO2001051661A3 - A universal nucleic acid amplification system for nucleic acids in a sample - Google Patents

A universal nucleic acid amplification system for nucleic acids in a sample Download PDF

Info

Publication number
WO2001051661A3
WO2001051661A3 PCT/NL2001/000020 NL0100020W WO0151661A3 WO 2001051661 A3 WO2001051661 A3 WO 2001051661A3 NL 0100020 W NL0100020 W NL 0100020W WO 0151661 A3 WO0151661 A3 WO 0151661A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sample
pathogen
primers
acid amplification
Prior art date
Application number
PCT/NL2001/000020
Other languages
French (fr)
Other versions
WO2001051661A2 (en
Inventor
Jing Zhang
John Dekker
Der Kuyl Antoinette Cornel Van
Jolanda Maas
Gemen Bob Van
Original Assignee
Amsterdam Support Diagnostics
Jing Zhang
John Dekker
Der Kuyl Antoinette Cornel Van
Jolanda Maas
Gemen Bob Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amsterdam Support Diagnostics, Jing Zhang, John Dekker, Der Kuyl Antoinette Cornel Van, Jolanda Maas, Gemen Bob Van filed Critical Amsterdam Support Diagnostics
Priority to EP01904651A priority Critical patent/EP1276901A2/en
Priority to AU2001232485A priority patent/AU2001232485A1/en
Publication of WO2001051661A2 publication Critical patent/WO2001051661A2/en
Priority to US10/192,786 priority patent/US6808888B2/en
Publication of WO2001051661A3 publication Critical patent/WO2001051661A3/en
Priority to US10/915,953 priority patent/US20050064478A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Abstract

The invention provides in one embodiment a method for amplifying nucleic acid in a sample comprising providing said sample with a set of primers to enable synthesizing at least one nucleic acid strand complementary to at least part of said nucleic acid, wherein said set of primers comprises between 3-8 random bases preferably clustered near the 3' end of each primer in said set of primers. In a preferred embodiment the method is used for determining whether a sample, derived from a patient, mammal, poultry and fish, comprises nucleic acid of a pathogen. The method is further suited for the typing of said pathogen and the typing of a particular variant of said pathogen. The method is also suited for the elucidation of the gene-expression profile or genetic profile of cells.
PCT/NL2001/000020 2000-01-13 2001-01-15 A universal nucleic acid amplification system for nucleic acids in a sample WO2001051661A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01904651A EP1276901A2 (en) 2000-01-13 2001-01-15 A universal nucleic acid amplification system for nucleic acids in a sample
AU2001232485A AU2001232485A1 (en) 2000-01-13 2001-01-15 A universal nucleic acid amplification system for nucleic acids in a sample
US10/192,786 US6808888B2 (en) 2000-01-13 2002-07-10 Universal nucleic acid amplification system for nucleic acids in a sample
US10/915,953 US20050064478A1 (en) 2000-01-13 2004-08-10 Universal nucleic acid amplification system for nucleic acids in a sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00200109.7 2000-01-13
EP00200109 2000-01-13

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/192,786 Continuation US6808888B2 (en) 2000-01-13 2002-07-10 Universal nucleic acid amplification system for nucleic acids in a sample

Publications (2)

Publication Number Publication Date
WO2001051661A2 WO2001051661A2 (en) 2001-07-19
WO2001051661A3 true WO2001051661A3 (en) 2002-10-24

Family

ID=8170905

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2001/000020 WO2001051661A2 (en) 2000-01-13 2001-01-15 A universal nucleic acid amplification system for nucleic acids in a sample

Country Status (4)

Country Link
US (2) US6808888B2 (en)
EP (1) EP1276901A2 (en)
AU (1) AU2001232485A1 (en)
WO (1) WO2001051661A2 (en)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1276901A2 (en) * 2000-01-13 2003-01-22 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
US7718354B2 (en) 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US20040121314A1 (en) * 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in containers
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
US20030027135A1 (en) * 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) * 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
EP2241636A1 (en) * 2002-03-13 2010-10-20 Genomic Health, Inc. Gene expression profiling in biopsied tumor tissues
US7171622B2 (en) 2002-07-18 2007-01-30 International Business Machines Corporation Method, apparatus and computer program product for projecting objects in a display unit
WO2004046386A1 (en) * 2002-11-15 2004-06-03 Genomic Health, Inc. Gene expression profiling of egfr positive cancer
JP2006516193A (en) 2002-12-06 2006-06-29 アイシス・ファーマシューティカルス・インコーポレーテッド Rapid identification of pathogens in humans and animals
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
AU2004211955B2 (en) * 2003-02-06 2009-05-14 Cedars-Sinai Medical Center Gene expression markers for response to EGFR inhibitor drugs
JP4568716B2 (en) * 2003-02-20 2010-10-27 ジェノミック ヘルス， インコーポレイテッド Use of intron RNA to measure gene expression
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1604040B1 (en) 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US8046171B2 (en) * 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
AU2004248120B2 (en) * 2003-05-28 2009-04-23 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
EP2226396A1 (en) * 2003-05-30 2010-09-08 Genomic Health, Inc. Gene expression markers for response to EGFR inhibitor drugs
PL3470535T3 (en) 2003-06-24 2020-08-24 Genomic Health, Inc. Prediction of likelihood of cancer recurrence
ES2651849T3 (en) * 2003-07-10 2018-01-30 Genomic Health, Inc. Expression profile and test algorithm for cancer prognosis
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20060240412A1 (en) * 2003-09-11 2006-10-26 Hall Thomas A Compositions for use in identification of adenoviruses
US20120122096A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US20100035239A1 (en) * 2003-09-11 2010-02-11 Isis Pharmaceuticals, Inc. Compositions for use in identification of bacteria
AU2004284434A1 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. qRT-PCR assay system for gene expression profiling
ES2360113T3 (en) * 2003-12-23 2011-06-01 Genomic Health, Inc. UNIVERSAL AMPLIFICATION OF FRAGMENTED RNA.
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) * 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
US8440404B2 (en) 2004-03-08 2013-05-14 Rubicon Genomics Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
WO2005086934A2 (en) * 2004-03-10 2005-09-22 Board Of Regents, The University Of Texas System Enriched cdna expression libraries and methods of making and using same
CA2563074C (en) 2004-04-09 2014-05-20 Genomic Health, Inc. Gene expression markers for predicting response to chemotherapy
EP1766659A4 (en) 2004-05-24 2009-09-30 Ibis Biosciences Inc Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
JP4939425B2 (en) 2004-11-05 2012-05-23 ジェノミック ヘルス， インコーポレイテッド Molecular indicators of prognosis and prediction of treatment response in breast cancer
PL1836629T3 (en) * 2004-11-05 2020-06-15 Genomic Health, Inc. Predicting response to chemotherapy using gene expression markers
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
CA2600184A1 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
JP2009502137A (en) * 2005-07-21 2009-01-29 アイシス ファーマシューティカルズ インコーポレイティッド Method for rapid identification and quantification of nucleic acid variants
WO2007018602A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
DK1924704T3 (en) 2005-08-02 2011-09-05 Rubicon Genomics Inc Compositions and Methods for Processing and Multiplying DNA, including Using Multiple Enzymes in a Single Reaction
CA2626977A1 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
WO2007067907A1 (en) 2005-12-06 2007-06-14 Ambion, Inc. Reverse transcription primers and methods of design
EP2010679A2 (en) 2006-04-06 2009-01-07 Ibis Biosciences, Inc. Compositions for the use in identification of fungi
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
CA2663029C (en) 2006-09-14 2016-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
EP2121956B1 (en) 2006-12-21 2016-08-17 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
WO2008118809A1 (en) * 2007-03-23 2008-10-02 Ibis Biosciences, Inc. Compositions for use in identification of mixed populations of bioagents
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
CA2707436C (en) 2007-06-29 2014-01-28 Epicentre Technologies Corporation Copy dna and sense rna
EP2347254A2 (en) 2008-09-16 2011-07-27 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
WO2010033625A1 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
WO2010104798A1 (en) 2009-03-08 2010-09-16 Ibis Biosciences, Inc. Bioagent detection methods
US9393564B2 (en) 2009-03-30 2016-07-19 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
WO2011008972A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systems for bioagent identification
WO2011008971A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Lift and mount apparatus
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
WO2011017656A2 (en) 2009-08-06 2011-02-10 Ibis Biosciences, Inc. Non-mass determined base compositions for nucleic acid detection
US20110189679A1 (en) * 2009-09-11 2011-08-04 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
WO2011041695A1 (en) * 2009-10-02 2011-04-07 Ibis Biosciences, Inc. Determination of methylation status of polynucleotides
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
WO2011115840A2 (en) 2010-03-14 2011-09-22 Ibis Biosciences, Inc. Parasite detection via endosymbiont detection
CN103154015A (en) * 2010-03-29 2013-06-12 韩建 Method for improving detection of b cell immunoglobulin gene recombination
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
DK3590949T3 (en) 2010-10-01 2022-07-11 Modernatx Inc RIBONUCLEIC ACIDS CONTAINING N1-METHYL-PSEUDOURACIL AND USE THEREOF
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
CN102952895B (en) * 2011-08-23 2014-12-17 中国科学院上海生命科学研究院 Method for detecting unknown viruses through utilizing sequencing technology
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
SG10201602654SA (en) 2011-10-03 2016-05-30 Moderna Therapeutics Inc Modified nucleosides,nucleotides,and nucleic acids,and uses thereof
WO2013090648A1 (en) 2011-12-16 2013-06-20 modeRNA Therapeutics Modified nucleoside, nucleotide, and nucleic acid compositions
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
JP2015516143A (en) 2012-04-02 2015-06-08 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． Modified polynucleotides for the production of proteins associated with human disease
LT2922554T (en) 2012-11-26 2022-06-27 Modernatx, Inc. Terminally modified rna
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
AU2014329452B2 (en) 2013-10-03 2019-06-20 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0466520A1 (en) * 1990-07-13 1992-01-15 Life Technologies Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
WO1996015264A2 (en) * 1994-11-12 1996-05-23 Jsd Technologies Ltd. Method for identifying nucleic acid sequences from different biological sources through amplification of the same
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
WO1998002575A1 (en) * 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
EP1276901A2 (en) * 2000-01-13 2003-01-22 Amsterdam Support Diagnostics B.V. A universal nucleic acid amplification system for nucleic acids in a sample
AU2002213882A1 (en) 2000-09-08 2002-03-22 Biomerieux B.V.A. Attenuated hiv strains and use thereof
US6794141B2 (en) * 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
EP0466520A1 (en) * 1990-07-13 1992-01-15 Life Technologies Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
WO1996015264A2 (en) * 1994-11-12 1996-05-23 Jsd Technologies Ltd. Method for identifying nucleic acid sequences from different biological sources through amplification of the same
US5948649A (en) * 1994-11-12 1999-09-07 Biotec Laboratories Limited Method for identifying nucleic acid sequences from different biological sources through amplification of the same
WO1997030062A1 (en) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation and amplification of nucleic acid materials
WO1998002575A1 (en) * 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MCGRATH A ET AL: "Sequence analysis of DNA randomly amplified from Saccharomyces genome", MOLECULAR AND CELLULAR PROBES, vol. 12, 1998, pages 397 - 405, XP002141297 *
ROSE TM ET AL: "Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences", NUCLEIC ACID RESEARCH, vol. 26, no. 7, 1998, pages 1628 - 35, XP002141299 *
SMITH N ET AL: "Automated differential display using a fluorescently labeled universal primer", BIOTECHNIQUES, vol. 23, no. 2, 1997, pages 274 - 79, XP002141296 *

Also Published As

Publication number Publication date
US6808888B2 (en) 2004-10-26
US20030073112A1 (en) 2003-04-17
EP1276901A2 (en) 2003-01-22
US20050064478A1 (en) 2005-03-24
WO2001051661A2 (en) 2001-07-19
AU2001232485A1 (en) 2001-07-24

Similar Documents

Publication Publication Date Title
WO2001051661A3 (en) A universal nucleic acid amplification system for nucleic acids in a sample
WO2002103011A3 (en) Selective gene amplification
WO1996023079A3 (en) Method for suppressing dna fragment amplification during pcr
CA2190072A1 (en) Fluorescence Polarization Detection of Nucleic Acid Amplification
WO2005001055A3 (en) Stabilized immunomodulatory oligonucleotides
WO2002000928A3 (en) Diagnosis of diseases associated with the immune system by determining cytosine methylation
EP2009102A3 (en) Random mutagenesis and amplification of nucleic acid
WO2002057491A3 (en) Direct multiplex characterization of genomic dna
CA2359180A1 (en) Method and medicament for inhibiting the expression of a given gene
WO2004044140A3 (en) 2’-substituted oligomeric compounds and compositions for use in gene modulations
AU6846698A (en) Method of nucleic acid amplification
EP1043405A3 (en) Proportional amplification of nucleic acids
EP2014776A3 (en) Diagnosis of diseases associated with DNA transcription
EP1445312A4 (en) Method of inhibiting gene expression
EP0774518A3 (en) Nucleic acid probes complementary to Human Papillomavirus nucleic acid and related methods and kits
WO2002046465A3 (en) Method for identification of genes involved in specific diseases
WO2003052110A3 (en) Nucleic acid nematicides
EP0822256A3 (en) Modified thermostable DNA polymerase, and DNA polymerase composition for nucleic acid amplification
AU2002365912A1 (en) Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
WO2001018248A3 (en) Quorum sensing signaling in bacteria
WO2002052041A3 (en) 5' nuclease nucleic acid amplification assay having an improved internal control
WO2002063049A3 (en) Methods for determining a nucleotide at a specific location within a nucleic acid molecule
WO2002036814A3 (en) Diagnosis of diseases associated with cdk4 by determining the methylation state of the cdk4
AU1908100A (en) Isothermal nucleic acid amplification methods
WO2002081644A3 (en) Haplotyping method for multiple distal nucleotide polymorphisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001904651

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10192786

Country of ref document: US

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2001904651

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP