WO2001027242A3 - Methods for identifying inhibitors of methionine aminopeptidases - Google Patents

Methods for identifying inhibitors of methionine aminopeptidases Download PDF

Info

Publication number
WO2001027242A3
WO2001027242A3 PCT/US2000/041146 US0041146W WO0127242A3 WO 2001027242 A3 WO2001027242 A3 WO 2001027242A3 US 0041146 W US0041146 W US 0041146W WO 0127242 A3 WO0127242 A3 WO 0127242A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
map
peptide
methionine
identifying inhibitors
Prior art date
Application number
PCT/US2000/041146
Other languages
French (fr)
Other versions
WO2001027242A2 (en
Inventor
Yie-Hwa Chang
Original Assignee
Univ Saint Louis
Chang Yie Hwa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Saint Louis, Chang Yie Hwa filed Critical Univ Saint Louis
Priority to DE60030744T priority Critical patent/DE60030744T2/en
Priority to CA002387126A priority patent/CA2387126A1/en
Priority to JP2001530447A priority patent/JP3727269B2/en
Priority to EP00984526A priority patent/EP1221954B1/en
Priority to AU21130/01A priority patent/AU2113001A/en
Publication of WO2001027242A2 publication Critical patent/WO2001027242A2/en
Publication of WO2001027242A3 publication Critical patent/WO2001027242A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods are provided for detecting methionine aminopeptidase (MAP) activity and for detecting inhibitors of MAP. The methods utilize a peptide comprising an N-terminal methionine which can be cleaved from the peptide by MAP, and a C-terminal detection moiety which is released by a second peptidase only if the N-terminal methionine has been cleaved from the peptide. When the peptide is combined with MAP and the second peptidase, the detection moiety is released, while the addition of a MAP inhibitor will inhibit the release of the detection moiety. Reaction mixes, peptides, and kits which are useful for practicing the methods are also provided.
PCT/US2000/041146 1999-10-14 2000-10-12 Methods for identifying inhibitors of methionine aminopeptidases WO2001027242A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE60030744T DE60030744T2 (en) 1999-10-14 2000-10-12 METHOD OF IDENTIFYING INHIBITORS OF METHIONINAMINOPEPTIDASES
CA002387126A CA2387126A1 (en) 1999-10-14 2000-10-12 Methods for identifying inhibitors of methionine aminopeptidases
JP2001530447A JP3727269B2 (en) 1999-10-14 2000-10-12 Method for identifying methionine aminopeptidase inhibitors
EP00984526A EP1221954B1 (en) 1999-10-14 2000-10-12 Methods for identifying inhibitors of methionine aminopeptidases
AU21130/01A AU2113001A (en) 1999-10-14 2000-10-12 Methods for identifying inhibitors of methionine aminopeptidases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/418,135 US6261794B1 (en) 1999-10-14 1999-10-14 Methods for identifying inhibitors of methionine aminopeptidases
US09/418,135 1999-10-14

Publications (2)

Publication Number Publication Date
WO2001027242A2 WO2001027242A2 (en) 2001-04-19
WO2001027242A3 true WO2001027242A3 (en) 2001-12-20

Family

ID=23656861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/041146 WO2001027242A2 (en) 1999-10-14 2000-10-12 Methods for identifying inhibitors of methionine aminopeptidases

Country Status (8)

Country Link
US (2) US6261794B1 (en)
EP (1) EP1221954B1 (en)
JP (1) JP3727269B2 (en)
AT (1) ATE339210T1 (en)
AU (1) AU2113001A (en)
CA (1) CA2387126A1 (en)
DE (1) DE60030744T2 (en)
WO (1) WO2001027242A2 (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1386279A2 (en) * 2001-04-03 2004-02-04 Smithkline Beecham Corporation Method for inhibiting metap2
US20020182701A1 (en) * 2001-08-30 2002-12-05 Saint Louis University Dominant negative variants of methionine aminopeptidase 2 (MetAP2) and clinical uses thereof
JP2006504627A (en) * 2002-03-27 2006-02-09 スミスクライン・ビーチャム・コーポレイション Compounds and methods
US7195884B2 (en) * 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
CN1894234A (en) 2003-03-25 2007-01-10 武田药品工业株式会社 Dipeptidyl peptidase inhibitors
EP1625122A1 (en) 2003-05-14 2006-02-15 Takeda San Diego, Inc. Dipeptidyl peptidase inhibitors
US7678909B1 (en) 2003-08-13 2010-03-16 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
US7169926B1 (en) 2003-08-13 2007-01-30 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
MXPA06001601A (en) 2003-08-13 2006-08-25 Takeda Pharmaceutical 4-pyrimidone derivatives and their use as peptidyl peptidase inhibitors.
WO2005026148A1 (en) 2003-09-08 2005-03-24 Takeda San Diego, Inc. Dipeptidyl peptidase inhibitors
CN1894273B (en) * 2003-12-01 2011-01-12 明治乳业株式会社 Peptide inhibiting angiotensin converting enzyme
US7732446B1 (en) 2004-03-11 2010-06-08 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
CN102079743B (en) 2004-03-15 2020-08-25 武田药品工业株式会社 Dipeptidyl peptidase inhibitors
US7109015B2 (en) * 2004-03-29 2006-09-19 Academia Sinica Removal of N-terminal methionine from proteins by engineered methionine aminopeptidase
WO2005118555A1 (en) 2004-06-04 2005-12-15 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
WO2006019965A2 (en) 2004-07-16 2006-02-23 Takeda San Diego, Inc. Dipeptidyl peptidase inhibitors
WO2006068978A2 (en) * 2004-12-21 2006-06-29 Takeda Pharmaceutial Company Limited Dipeptidyl peptidase inhibitors
WO2006114193A1 (en) * 2005-04-28 2006-11-02 Unilever N.V. Peptides having an ace inhibiting effect
PE20070622A1 (en) * 2005-09-14 2007-08-22 Takeda Pharmaceutical ADMINISTRATION OF DIPEPTIDYL PEPTIDASE INHIBITORS
WO2007033350A1 (en) 2005-09-14 2007-03-22 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors for treating diabetes
EP1924567B1 (en) 2005-09-16 2012-08-22 Takeda Pharmaceutical Company Limited Process for the preparation of pyrimidinedione derivatives
US7740691B2 (en) * 2006-01-10 2010-06-22 Edwin W. Cash Gas treating method and apparatus
WO2007112347A1 (en) 2006-03-28 2007-10-04 Takeda Pharmaceutical Company Limited Dipeptidyl peptidase inhibitors
CA2663279C (en) * 2006-09-13 2016-05-17 Takeda Pharmaceutical Company Limited Use of 2-6-(3-amino-piperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydro-2h-pyrimidin-1-ylmethyl-4-fluoro-benzonitrile for treating diabetes, cancer, autoimmune disorders and hiv infection
US8324383B2 (en) 2006-09-13 2012-12-04 Takeda Pharmaceutical Company Limited Methods of making polymorphs of benzoate salt of 2-[[6-[(3R)-3-amino-1-piperidinyl]-3,4-dihydro-3-methyl-2,4-dioxo-1(2H)-pyrimidinyl]methyl]-benzonitrile
TW200838536A (en) 2006-11-29 2008-10-01 Takeda Pharmaceutical Polymorphs of succinate salt of 2-[6-(3-amino-piperidin-1-yl)-3-methyl-2,4-dioxo-3,4-dihydro-2H-pyrimidin-1-ylmethy]-4-fluor-benzonitrile and methods of use therefor
US8093236B2 (en) 2007-03-13 2012-01-10 Takeda Pharmaceuticals Company Limited Weekly administration of dipeptidyl peptidase inhibitors
US20100144140A1 (en) * 2008-12-10 2010-06-10 Novellus Systems, Inc. Methods for depositing tungsten films having low resistivity for gapfill applications
WO2013125622A1 (en) * 2012-02-22 2013-08-29 森永乳業株式会社 Dipeptidyl peptidase-iv inhibitor

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999018856A1 (en) * 1997-10-10 1999-04-22 Cytovia, Inc. Novel fluorescent reporter molecules and their applications including assays for caspases
US5985273A (en) * 1994-06-17 1999-11-16 Reed; Benjamin J. Biological control of insects
US6184020B1 (en) * 1997-12-16 2001-02-06 Novo Nordisk Biotech, Inc. Polypeptides having aminopeptidase activity and nucleic acids encoding same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5255593A (en) * 1975-10-30 1977-05-07 Ajinomoto Kk Measuring method of enzyme activity
NL7607684A (en) * 1976-07-12 1978-01-16 Akzo Nv PROCESS FOR PREPARING NEW PEPTIDES AND PEPTIDE DERIVATIVES AND THEIR USE
US5268164A (en) * 1990-04-23 1993-12-07 Alkermes, Inc. Increasing blood-brain barrier permeability with permeabilizer peptides
NL9401886A (en) * 1994-05-27 1996-01-02 Dsm Nv Composition consisting of a dendrimer and an active substance contained in the dendrimer, a method of preparing such a composition and a method of releasing the active substance.
US5888796A (en) 1996-01-31 1999-03-30 St. Louis University Clone of a nucleotide sequence encoding a protein having two functions
US6136604A (en) * 1999-10-27 2000-10-24 Isis Pharmaceuticals Inc. Antisense inhibition of methionine aminopeptidase 2 expression

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985273A (en) * 1994-06-17 1999-11-16 Reed; Benjamin J. Biological control of insects
WO1999018856A1 (en) * 1997-10-10 1999-04-22 Cytovia, Inc. Novel fluorescent reporter molecules and their applications including assays for caspases
US6184020B1 (en) * 1997-12-16 2001-02-06 Novo Nordisk Biotech, Inc. Polypeptides having aminopeptidase activity and nucleic acids encoding same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
IKEHARA Y. ET AL.: "Dipeptidyl peptidase IV from rat liver", METHODS IN ENZYMOLOGY, vol. 244, 1994, pages 215 - 227, XP002940358 *

Also Published As

Publication number Publication date
WO2001027242A2 (en) 2001-04-19
ATE339210T1 (en) 2006-10-15
EP1221954B1 (en) 2006-09-13
EP1221954A4 (en) 2002-11-27
JP3727269B2 (en) 2005-12-14
CA2387126A1 (en) 2001-04-19
DE60030744T2 (en) 2007-09-20
US6593454B2 (en) 2003-07-15
DE60030744D1 (en) 2006-10-26
EP1221954A2 (en) 2002-07-17
AU2113001A (en) 2001-04-23
US20010047078A1 (en) 2001-11-29
US6261794B1 (en) 2001-07-17
JP2003511063A (en) 2003-03-25

Similar Documents

Publication Publication Date Title
WO2001027242A3 (en) Methods for identifying inhibitors of methionine aminopeptidases
WO2001092579A3 (en) Methods for detecting target nucleic acids using coupled ligation and amplification
AU6162999A (en) Apoptosis marker antibodies and methods of use
AU8198091A (en) Method for amplifying a signal emitted by a luminescent compound
GB9812523D0 (en) Peptide inhibitors of hepatitis c virus ns3 protease
WO2004034033A3 (en) Methods and systems for detecting mhc class i and class ii binding peptides
WO2003035897A3 (en) Enzyme substrates for detecting beta-d-ribofuranosidase activity
WO2002006458A3 (en) Protease specific cleavable luciferases and methods of use thereof
EP1298205A4 (en) Novel lipase
AU2126400A (en) Detection of small molecules by use of a piezoelectric sensor
WO2002031509A3 (en) Method for determining mass altering moiety in peptides
WO2000068414A3 (en) Whole cell assay for cathepsin k
WO2002028895A3 (en) Cyclic peptides, method for preparing same and use as angiogenesis inhibitor or activator
WO2008146259A3 (en) Malt1 specific cleavage in assay and screening method
AU2002315637A1 (en) Displacement assay for detecting ligate-ligand association events
WO2002038799A3 (en) Detection of proteases and screening for protease inhibitors
WO2002058533A3 (en) Process for determining target function and identifying drug leads
WO2004099240A3 (en) Peptides and mixtures thereof for use in the detection of severe acute respiratory syndrome-associated coronavirus (sars)
ATE455862T1 (en) IMPROVED RECEPTOR DETECTION
EP1313876B8 (en) Process for detecting acetylase or deacetylase activity in an immunoassay
Rush et al. Fluorogenic substrates for the enkephalin-degrading neutral endopeptidase (Enkephalinase)
AU2002220797A1 (en) Method for identifying listeria monocytogenes and culture medium
TW200509911A (en) Novel combination
ATE349699T1 (en) METHOD FOR IDENTIFYING MODULATORS OF PROTEASE-ACTIVATED RECEPTORS (PAR)
WO2004038412A3 (en) Method for detecting halitosis

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2387126

Country of ref document: CA

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 530447

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000984526

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000984526

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 2000984526

Country of ref document: EP