WO2001007651A3 - Transposon mediated differential hybridisation - Google Patents

Transposon mediated differential hybridisation Download PDF

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Publication number
WO2001007651A3
WO2001007651A3 PCT/GB2000/002879 GB0002879W WO0107651A3 WO 2001007651 A3 WO2001007651 A3 WO 2001007651A3 GB 0002879 W GB0002879 W GB 0002879W WO 0107651 A3 WO0107651 A3 WO 0107651A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
library
organism
transposon mediated
differential hybridisation
Prior art date
Application number
PCT/GB2000/002879
Other languages
French (fr)
Other versions
WO2001007651A2 (en
Inventor
Ian George Charles
Duncan John Maskell
Original Assignee
Arrow Therapeutics Ltd
Ian George Charles
Duncan John Maskell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arrow Therapeutics Ltd, Ian George Charles, Duncan John Maskell filed Critical Arrow Therapeutics Ltd
Priority to KR1020027001136A priority Critical patent/KR20020034166A/en
Priority to IL14784300A priority patent/IL147843A0/en
Priority to AU64544/00A priority patent/AU6454400A/en
Priority to CA002380171A priority patent/CA2380171A1/en
Priority to GB0204495A priority patent/GB2369676B/en
Priority to EP00951684A priority patent/EP1218540A2/en
Priority to JP2001512917A priority patent/JP2003505091A/en
Publication of WO2001007651A2 publication Critical patent/WO2001007651A2/en
Publication of WO2001007651A3 publication Critical patent/WO2001007651A3/en
Priority to US11/220,910 priority patent/US20060188897A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Abstract

A method for identifying an essential gene of an organism comprises: (i) providing a library of transposon mutants of the said organism; (ii) isolating polynucleotide sequences from the library which flank inserted transposons; (iii) hybridising the said polynucleotide sequences with a polynucleotide library from the said organism; and (iv) identifying a polynucleotide in the said polynucleotide library to which the said polynucleotide sequences do not hybridise, thereby to determine an essential gene of the organism.
PCT/GB2000/002879 1999-07-26 2000-07-26 Transposon mediated differential hybridisation WO2001007651A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
KR1020027001136A KR20020034166A (en) 1999-07-26 2000-07-26 Transposon Mediated Differential Hybridisation
IL14784300A IL147843A0 (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation
AU64544/00A AU6454400A (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation
CA002380171A CA2380171A1 (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation
GB0204495A GB2369676B (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation
EP00951684A EP1218540A2 (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation
JP2001512917A JP2003505091A (en) 1999-07-26 2000-07-26 Transposon-mediated differential hybridization
US11/220,910 US20060188897A1 (en) 1999-07-26 2005-09-08 Transposon mediated differential hybridisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9917531.7 1999-07-26
GBGB9917531.7A GB9917531D0 (en) 1999-07-26 1999-07-26 Transposon mediated differential hybridisation

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10031786 A-371-Of-International 2002-03-21
US10/356,733 Continuation-In-Part US20040006434A1 (en) 1999-07-26 2003-02-03 Method for the identification of essential and conditional essential genes

Publications (2)

Publication Number Publication Date
WO2001007651A2 WO2001007651A2 (en) 2001-02-01
WO2001007651A3 true WO2001007651A3 (en) 2002-05-02

Family

ID=10857957

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002879 WO2001007651A2 (en) 1999-07-26 2000-07-26 Transposon mediated differential hybridisation

Country Status (8)

Country Link
EP (1) EP1218540A2 (en)
JP (1) JP2003505091A (en)
KR (1) KR20020034166A (en)
AU (1) AU6454400A (en)
CA (1) CA2380171A1 (en)
GB (2) GB9917531D0 (en)
IL (1) IL147843A0 (en)
WO (1) WO2001007651A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004529644A (en) * 2001-04-10 2004-09-30 エクセリクシス・インコーポレイテッド Insect Attack Vectors and Methods of Use for Identification of Pesticide Targets
US8173363B2 (en) 2002-08-20 2012-05-08 Novartis Vaccines And Diagnostics, Inc. Random transposon insertion in Staphylococcus aureus and use thereof to identify essential genes
FI20030561A0 (en) * 2003-04-14 2003-04-14 Finnzymes Oy A method for transferring nucleic acids into eukaryotic genomes
GB0321232D0 (en) * 2003-09-10 2003-10-08 Arrow Therapeutics Ltd Method for the identification of attenuating lesions in pathogens
GB201107516D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets by complemented sequencing
GB201107515D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets
GB201219989D0 (en) 2012-11-06 2012-12-19 Discuva Ltd Bacterial engineering
GB201413202D0 (en) 2014-07-25 2014-09-10 Discuva Ltd Process for producing bacterial mutants
GB201413198D0 (en) 2014-07-25 2014-09-10 Discuva Ltd Methods For Characterizing Bacterial Mutants

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733760A (en) * 1994-08-05 1998-03-31 Virus Research Institute Salmonella vectors encoding truncated pag fusion protein, method of making, and uses thereof
WO1998020161A1 (en) * 1996-11-06 1998-05-14 Smithkline Beecham Corporation Methods for identifying genes essential to the growth of an organism
US6232074B1 (en) * 1999-12-10 2001-05-15 Complegen, Inc. Functional gene array in yeast

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6323074B1 (en) * 2000-04-24 2001-11-27 Taiwan Semiconductor Manufacturing Company High voltage ESD protection device with very low snapback voltage by adding as a p+ diffusion and n-well to the NMOS drain

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733760A (en) * 1994-08-05 1998-03-31 Virus Research Institute Salmonella vectors encoding truncated pag fusion protein, method of making, and uses thereof
WO1998020161A1 (en) * 1996-11-06 1998-05-14 Smithkline Beecham Corporation Methods for identifying genes essential to the growth of an organism
US6232074B1 (en) * 1999-12-10 2001-05-15 Complegen, Inc. Functional gene array in yeast

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHUN K T ET AL: "THE IDENTIFICATION OF TRANSPOSON-TAGGED MUTATIONS IN ESSENTIAL GENES THAT AFFECT CELL MORPHOLOGY IN SACCHAROMYCES CEREVISIAE", GENETICS, GENETICS SOCIETY OF AMERICA, AUSTIN, TX, US, vol. 142, no. 1, January 1996 (1996-01-01), pages 39 - 50, XP000995589, ISSN: 0016-6731 *
NELSON K E ET AL: "IDENTIFICATION OF THE MINIMAL ESSENTIAL GENE SET OF HAEMOPHILUS INFLUENZAE VIA TRANSPOSON MUTAGENESIS", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 97, 4 May 1997 (1997-05-04), pages 321, XP000994943, ISSN: 0067-2777 *

Also Published As

Publication number Publication date
GB0204495D0 (en) 2002-04-10
GB2369676B (en) 2002-12-31
WO2001007651A2 (en) 2001-02-01
KR20020034166A (en) 2002-05-08
JP2003505091A (en) 2003-02-12
AU6454400A (en) 2001-02-13
GB2369676A (en) 2002-06-05
CA2380171A1 (en) 2001-02-01
IL147843A0 (en) 2002-08-14
EP1218540A2 (en) 2002-07-03
GB9917531D0 (en) 1999-09-29

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