WO2001000234A3 - Methods and compositions for engineering of attenuated vaccines - Google Patents

Methods and compositions for engineering of attenuated vaccines Download PDF

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Publication number
WO2001000234A3
WO2001000234A3 PCT/US2000/016984 US0016984W WO0100234A3 WO 2001000234 A3 WO2001000234 A3 WO 2001000234A3 US 0016984 W US0016984 W US 0016984W WO 0100234 A3 WO0100234 A3 WO 0100234A3
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WO
WIPO (PCT)
Prior art keywords
methods
organisms
attenuated vaccines
vaccines
engineering
Prior art date
Application number
PCT/US2000/016984
Other languages
French (fr)
Other versions
WO2001000234A2 (en
Inventor
Juha Punnonen
Russell Howard
Willem P C Stemmer
Stephen Delcardayre
Doris Apt
Original Assignee
Maxygen Inc
Juha Punnonen
Russell Howard
Willem P C Stemmer
Stephen Delcardayre
Doris Apt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/344,655 external-priority patent/US6121736A/en
Application filed by Maxygen Inc, Juha Punnonen, Russell Howard, Willem P C Stemmer, Stephen Delcardayre, Doris Apt filed Critical Maxygen Inc
Priority to CA002377084A priority Critical patent/CA2377084A1/en
Priority to AU58809/00A priority patent/AU5880900A/en
Priority to EP00944760A priority patent/EP1196552A2/en
Priority to JP2001505941A priority patent/JP2003503039A/en
Publication of WO2001000234A2 publication Critical patent/WO2001000234A2/en
Publication of WO2001000234A3 publication Critical patent/WO2001000234A3/en

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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • A61K2039/5254—Virus avirulent or attenuated
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    • C12N2770/10011—Arteriviridae
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Abstract

This invention provides attenuated vaccines, and methods of obtaining attenuated vaccines. The vaccines of the invention include recombinant viral, bacterial, parasite, and other organisms that are evolved to exhibit increased attenuation without loss of effectiveness as a vaccine. The methods involve the creation of libraries of recombinant nucleic acids (e.g., whole or partial genomes, or particular nucleic acids) which are introduced into the vaccine viruses or other organisms, followed by screening and/or selection for those viruses or organisms that are attenuated.
PCT/US2000/016984 1999-06-25 2000-06-20 Methods and compositions for engineering of attenuated vaccines WO2001000234A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002377084A CA2377084A1 (en) 1999-06-25 2000-06-20 Methods and compositions for engineering of attenuated vaccines
AU58809/00A AU5880900A (en) 1999-06-25 2000-06-20 Methods and compositions for engineering of attenuated vaccines
EP00944760A EP1196552A2 (en) 1999-06-25 2000-06-20 Methods and compositions for engineering of attenuated vaccines
JP2001505941A JP2003503039A (en) 1999-06-25 2000-06-20 Methods and compositions for the manipulation of attenuated vaccines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/344,655 1999-06-25
US09/344,655 US6121736A (en) 1998-07-10 1999-06-25 Control apparatus for motor, and motor unit having the control apparatus

Publications (2)

Publication Number Publication Date
WO2001000234A2 WO2001000234A2 (en) 2001-01-04
WO2001000234A3 true WO2001000234A3 (en) 2001-08-02

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PCT/US2000/016984 WO2001000234A2 (en) 1999-06-25 2000-06-20 Methods and compositions for engineering of attenuated vaccines

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EP (1) EP1196552A2 (en)
JP (1) JP2003503039A (en)
AU (1) AU5880900A (en)
CA (1) CA2377084A1 (en)
WO (1) WO2001000234A2 (en)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN108641993A (en) * 2018-05-17 2018-10-12 新疆农垦科学院 A kind of recombinant lactic acid bacteria oral vaccine strain of food-grade expression bovine viral diarrhea virus E2 albumen

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US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
EP2471937A3 (en) * 2005-11-24 2013-04-24 The Research Foundation for Microbial Diseases of Osaka University Recombinant polyvalent vaccine
MX2011003196A (en) * 2008-09-26 2011-04-27 Ambrx Inc Non-natural amino acid replication-dependent microorganisms and vaccines.
US9132185B2 (en) 2010-06-02 2015-09-15 Virginia Tech Intellectual Properties, Inc. Modified live-attenuated vaccines (MLV) created by DNA shuffling against porcine reproductive and respiratory syndrome virus (PRRSV)
WO2014095956A1 (en) * 2012-12-19 2014-06-26 Intervet International B.V. CANINE PARVOVIRUS TYPE 2c ISOLATES AND METHODS OF USE
JP2014236748A (en) * 2014-08-26 2014-12-18 一般財団法人阪大微生物病研究会 Recombinant polyvalent vaccine
WO2017187131A1 (en) * 2016-04-26 2017-11-02 London School Of Hygiene & Tropical Medicine Reoviridae vaccine
US10174295B1 (en) * 2017-08-01 2019-01-08 The Charles Stark Draper Laboratory, Inc. Composition of matter: engineering of Escherichia coli phage K1E
EP3873516A1 (en) * 2018-10-31 2021-09-08 Boehringer Ingelheim Vetmedica GmbH 4/91 ibv vaccine with heterologous spike protein
CN110184242B (en) * 2019-06-11 2021-08-20 武汉生物制品研究所有限责任公司 Mouse virulent challenge strain of Coxsackie virus group A6 (CVA6) and application thereof
GB202009701D0 (en) * 2020-06-25 2020-08-12 Theolytics Ltd Generation of diverse viral libraries
CN113061581A (en) * 2021-03-22 2021-07-02 华中农业大学 Goat-derived bovine herpes virus type 1 strain and application thereof
CN116036253B (en) * 2022-11-24 2023-12-26 畜科生物工程有限公司 Live vaccine for porcine reproductive and respiratory syndrome and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108641993A (en) * 2018-05-17 2018-10-12 新疆农垦科学院 A kind of recombinant lactic acid bacteria oral vaccine strain of food-grade expression bovine viral diarrhea virus E2 albumen

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