WO2000077168A2 - ANTAGONISTS OF BMP AND TGFβ SIGNALLING PATHWAYS - Google Patents

Abstract

This invention provides unique members of the Hect family of ubiquitin ligases that specifically target BMP and TGFβ/activin pathway-specific Smads. The novel ligases have been named Smurf1 and Smurf2. They directly interact with Smads1 and 5 and Smad7, respectively, and regulate the ubiquitination, turnover and activity of Smads and other proteins of these pathways. Smurf1 interferes with biological responses to BMP, but not activin signalling. In amphibian embryos Smurf1 inhibits endogenous BMP signals, resulting in altered pattern formation and cell fate specification in the mesoderm and ectoderm. The present invention provides a unique regulatory link between the ubiquitination pathway and the control of cell fate determination by the TGFβ superfamily during embryonic development. Thus, Smurf1 is a negative regulator of Smad1 signal transduction, by targeting Smad1, Smurf1 blocks BMP signalling. In mammalian cells, Smurf2 suppresses TGFβ signalling, and in Xenopus, blocks formation of dorsal mesoderm and causes anterior truncation of the embryos. Smurf2 forms a stable complex with Smad7, which induces degradation and downregulation of TGFβ/activin signalling.

Description

ANTAGONISTS OF BMP AND TGFβ SIGNALING PATHWAYS

The research leading to this invention was supported in part by The National Institute of Health Grant No. SR01HD3242902. Thus, the United States Government has certain rights in the invention.

BACKGROUND OF THE INVENTION

In virtually all animal phyla, critical steps in embryonic development are regulated by cell-to-cell, or inductive, signals mediated by secreted growth factors. In particular, members of the transforming growth factor β (TGFβ) superfamily regulate a myriad of cellular and developmental processes, such as mitosis, cell differentiation, embryonic pattern formation and organogenesis. In vertebrate embryos, a variety of TGFβ signals affect germ layer specification, body patterning, cell growth and differentiation (1- 4). In embryos of the amphibian Xenopus, distinct TGFβ members induce different cell fates, e.g., activin, Vgl and nodal induce mesoderm characteristic of the dorsal part of the embryo, such as notochord and muscle. Vgl and activin also induce endoderm characteristics. In contrast, Bone Moφhogenetic Proteins (BMPs) specify mesoderm, such as blood and mesenchyme, and regulate epidermal and neural cell differentiation in the ectoderm (see (5) for a review).

Cells respond to ligands in the TGFβ family by transducing signals directly from cell surface receptor complexes to nuclear DNA targets via the Smad family of proteins (see (4), (6), (7), and (52) for a review).

Smads are related to Drosophila Mad (mothers against decapentaplegic [dpp]) and proteins encoded by three related nematode genes Sma 2, Sma 3, and Sma 4. The terms Sma and Mad have been fused as Smad to unify the nomenclature. There are eight members in the Smad family. Phosphorylated Smads 1 , 5 and 8 are functional mediators of BMP family signaling in partnership with Smad 4. Smads 2 and 3 are signal transducers for actions of TGFβ and activins. Smad 6 and Smad 7 function as antagonists to inhibit TGFβ/BMP superfamily signaling. Interestingly, Smad7 is localized in the nucleus and accumulates in the cytoplasm in response to TGFβ signalling (73).

Furthermore, expression of both Smadό and Smad7 is regulated by TGFbs, BMPs, growth factors and cytokines thereby providing for negative feedback regulation of the Smad signalling pathway (53-58). Phosphorylated Smad 1 forms a heteromeric complex with Smad 4 when entering the nucleus and activates transcription of early response genes. The BMP receptors may also signal via the mitogen activated protein kinase. It is likely that BMPs regulate cell cycle progression and thus govern differentiation of mesenchymal stem cells.

Signal transduction in two major pathways, BMP and activin/TGFβ, have been described in detail. Two distinct receptor subunits, the Type I and II transmembrane serine/threonine kinases, form activated complexes upon ligand binding. In these complexes the Type II subunit activates the Type I subunit, which directly phosphorylates and activates particular receptor-regulated R-Smad proteins: BMP receptors target Smadl and closely-related Smads 5 and 8, while activin and TGFβ receptors target Smadl and closely-related Smads 2 and 3. Upon activation these R-Smads form a heteromeric complex with Smad4, the "common partner" Smad. This complex translocates to the nucleus, binds to promoters of target genes in cooperation with DNA binding proteins, and activates transcription by recruiting coactivators. A third class of inhibitory Smads (I-Smads), Smadό and Smad7, function as inhibitors that block Smad-Smad complex formation or Smad-receptor interactions. I-Smads bind to the cytoplasmic domain of receptors or directly to Smadl . Mutations in components at all levels in this pathway are associated with embryonic defects and various cancers, underscoring the importance of this growth factor family in developmental and disease processes. (See, (4), (7) for reviews.) In particular, defects of Smad2 and Smad4 are associated with colon and lung cancer and defects in human Smad4 are associated with pancreatic cancer. Smads do not have intrinsic enzymatic activity. Thus, the nature of the cellular response to Smad signaling is exquisitely sensitive to the level of Smad protein in the cell. Indeed, alternative cell fate determinations in Xenopus embryos can be achieved by relatively small changes in the amount of Smad protein expressed in the cell (8-11). Therefore, regulating the level of Smad protein in the cell can be used as one means of modulating morphogenetic signaling by the TGFβ superfamily.

Protein modification by covalent attachment of ubiquitin is recognized as a general signal to target proteins for degradation via the proteasome (see (12), and (13) for a review). Targets of selective ubiquitination include transcription factors, cell cycle regulators, signal transduction proteins, and membrane proteins (references in (12)). Selective ubiquitination and degradation of specific target proteins can function as an important mechanism to control cell cycle progression, programmed cell death, differentiation and embryonic development. Dysfunction of the ubiquitination pathway is associated with disease and abnormal development. Ubiquitin ligases are part of a multimeric complex that catalyzes the covalent attachment of ubiquitin, a 12.5 kD polypeptide, to target proteins. Attachment of ubiquitin to its target serves as a molecular "flag" that marks the ubiquitinated protein for proteolytic degradation via an organelle known as the 26S proteosome. There are at least three enzymes involved in conjugating ubiquitin to target proteins, namely, El, E2 and E3. The El enzyme activates a ubiquitin molecule and conjugates it to the E2 enzyme which then either directly attaches ubiquitin onto a target protein, or passes it to the E3 ubiquitin ligase. The E3 recognizes a particular substrate and directs it ubiquitination.

A few examples of developmental regulation by the ubiquitination system have been described in Dictyostelium (14-16) and Drosophila (17-21). Conjugation of ubiquitin to receptors is used in diverse systems to control endocytosis and signalling, as well as receptor steady state levels by both proteasome- and lysosome-mediated degradation (59-60). Direct ubiquitination of membrane receptors has been characterized in a number of systems, although in some cases ubiquitin-dependent regulation does not appear to involve direct conjugation of ubiquitin to the receptor (61-62). Although many cell surface receptors are regulated by ubiquitin-dependent pathways, few E3 ubiquitin ligases that bind to membrane proteins and target them for ubiquitination have been defined. Nedd4, a C2-WW-HECT domain E3 ubiquitin ligase, can regulate the turnover of the amiloride-sensitive sodium channel by binding directly to a PPXY motif present in the carboxy-terminus of the channel (63-66). Furthermore, the RING finger protein, c-cbl, has recently been shown to function as an E3 ubiquitin ligase that binds to the EGF receptor to mediate ubiquitination and downregulation of the receptor complex (67-68). In these examples, ubiquitination of the membrane proteins appears to involve direct interactions between the E3 ligase and the target protein. Whether adaptor proteins might also function to recruit E3 ligases to specific receptor complexes is unknown. The mechanism and targets of ubiquitination in the control of patterning have heretofore remained elusive. References that are cited throughout the specification by number are listed at the end of the Example. All references cited herein are incoφorated by reference.

SUMMARY OF THE INVENTION

The present invention advantageously provides a class of regulatory proteins that are involved in BMP and TGFβ-mediated activation. In particular, these proteins regulate Smad proteins and/or promote degradation of TGFβ receptor complexes in the presence of Smad proteins. By manipulating the activity of the proteins of the invention, the skilled artisan can up or down regulate cellular activation, e.g., via BMP or TGFβ.

Thus, in a first aspect, the invention provides an isolated Smurf protein, and particularly a human Smurf protein. In one embodiment, it is a Smurfl protein. In an alternative embodiment, it is a SmurfZ protein. In specific embodiments, exemplified infra, a human Smurfl has the amino acid sequence depicted in Figure 10 (SEQ ID NO:2), and a human Smurf2 has the amino acid sequence depicted in Figure 12 (SEQ ID NO:4). Smurf proteins of the invention may contain at least about 5 and preferably at least about 10 contiguous amino acids from the sequences depicted in SEQ ID NO:2 and 4.

The invention further provides an antibody that specifically binds to Smurf protein.

The invention further provides a nucleic acid encoding the Smurf proteins of the invention. In specific embodiments, the nucleic acid has a nucleotide sequence as depicted in SEQ ID NO:l or SEQ ID NO:3.

The invention further provides an oligonucleotide or nucleic acid that specifically hybridizes under highly stringent conditions to a nucleic acid having a sequence encoding Smurf, or the complementary sequence thereof. Such hybridizable nucleic acids include probes (i.e., they may be labeled), primers (e.g., for PCR amplification), anti-sense nucleic acids, ribozymes, and triple-helix forming nucleic acids. The invention further provides a vector comprising the nucleic acid encoding Smurf, e.g., under control of an expression control sequence. Also provided are host cells, harboring such a vector, and methods for producing Smurf by culturing such host cells under conditions that permit expression of Smurf protein from the vector. Also contemplated is a transgenic non-human animal that expresses a human Smurf protein and non-human animals in which endogenous Smurf protein is deleted.

The invention further provides a method for inhibiting a bone moφhogenic protein or transforming growth factor-beta activation pathway in a cell. This method comprises permitting the cell to grow under conditions that permit expression of Smurf from a vector of introduced into the cell. Alternatively, the invention provides a method for promoting a bone moφhogenic protein or transforming growth factor-beta activation pathway in a cell, which method comprises suppressing expression of endogenous Smurf in the cell.

In addition, the present discoveries permit screening for a modulator of Smurf activity. Screens of the invention comprise detecting modulation of Smurf activity in the host cell in the presence of a test compound relative to Smurf activity of the host cell in the absence of the test compound. As shown in the examples, one such activity is ubiquitination of Smad proteins. Another activity is the enhancement of TGFβ receptor degradation.

DESCRIPTION OF THE DRAWINGS Figure 1. Smurfl encodes an E3 ubiquitin ligase. Protein sequence of Xenopus and human Smurfl compared to yeast (S. pombe) publ, given as SMURFl, hSMURFl and PUB1, respectively in the figure. Identical amino acids are shaded dark gray and conservative substitutions are shaded light gray. Based on primary structure Smurfl and publ are members of the Hect family of E3 ubiquitin ligases and display several conserved features of the family: A Iipid/Ca2+ binding domain is located at the N-terminus (residues 22-37), two WW protein interaction domains at 236 - 271 and 282 -311 (indicated by thick lines) , and a catalytic Hect domain beginning at residue 347 and extending to the C-terminus. Alignment was by Clustal W analysis (Mac Vector).

Figures 2A and 2B. Northern blots ofmSmurfl expression in embryonic and adult mouse tissues . Equal amounts of Poly A+ mRNA from indicated stages and tissues were analyzed. (2A) Embryonic tissue - In this blot, embryonic days post coitum are indicated. (2B) Adult tissues shown are T, testes; K, kidney; M, skeletal muscle; L, lung; Sp, spleen; Br, brain; H, heart.

Figures 3A and 3B. Developmental expression of Xenopus Smurfl. (A) RT-PCR on staged embryonic cDNA revealed that Xenopus Smurfl is a maternal mRNA, present at highest levels in egg, blastula and early gastrula stages. Zygotic Smurfl mRNA levels decline at gastrulation but maintain steady expression into swimming tadpole stages. Numbers above each lane correspond to Nieuwkoop and Faber stages: 7 and 9, blastula; 1 1 and 13, gastrula; 15 and 20, neurula; 25 and 35, tadpole. Ornithine decarboxylase (ODC) mRNA, ubiquitously expressed in cells, was assayed to normalize for RNA recovery. RT-PCR on mock cDNA (no reverse transcriptase). (B) Whole-mount in situ hybridization of Smurfl in developing Xenopus embryos. In egg and blastula stages Smurfl transcripts are localized to the animal pole half (bracket in egg). Expression is diffuse throughout the ectoderm and involuting marginal zone of the gastrula; views from , animal pole (an) and vegetal pole (Veg). There is some enrichment of transcripts in the neural folds at neurula stage 17. At tadpole stages 25 and 35 Smurfl expression includes the brain (b), eye (e), otic vesicle (o), somites (s), pharyngeal pouches (p) and developing kidney (k).

Figures 4A, 4B, 4C, and 4D. Smurfl leads to a selective decrease in steady-state level of Smadl and SmadS in mammalian cell lines. Cells were transiently transfected with the indicated expression vectors (DNA quantities in μg) and two days later an aliquot representing approximately 0.4% of total cell lysates were subjected to SDS-PAGE and immunoblotting. To determine the steady-state level of the Smads, the blots were probed with the appropriate Smad antibody as shown. Flag-hSmurfl expression level was confirmed by probing blots of total cell lysates with the anti-Flag monoclonal antibody. (A) COS-1 or 293T-cells were transfected with a constant amount of pCMV5-Smadl and increasing concentrations of pCMV5-Flag-hSmurfl as indicated. To determine Smadl steady- state level and the expression of hSmurfl, Western blots of total cell lysates from both cell lines were probed with α-Smadl and α-Flag antibodies (α-Smadl and α-Flag blot). (B) 293T cells were transiently transfected with pCMV5-Smadl, wild type or activated (Q203D) pCMV5-ALK6-HA, and increasing amounts of pCMV5-Flag-hSmurfl as indicated. Smadl steady-state levels were examined by immunoblotting total cell lysates in Western blots with α-Smadl antibody (α-Smadl). hSmurfl and wild type or activated ALK6 expression levels were determined by immunoblotting the total cell lysates with α-Flag (α-Flag) or α-HA (α-HA) antibody, respectively.

(C) 293T cells were transfected with a constant amount of pCMV5-Smadl or pCMV5-Smad2 along with the indicated concentrations of pCMV5-Flag-hSmurfl . Smadl (α-Smadl blot), Smad2 (α-Smad2 blot) and Flag-hSmurfl (α-Flag blot) steady-state protein levels were determined in cell lysates, as above. (D) 293T cells were transfected with Smadl, Smad3, Smad4 or Smad5 in pCMV5 in the absence or presence of pCMV5-Flag-hSmurfl . The steady-state level of the Smads in total cell lysates was determined by incubating the Western blots with α-Smadl antibody for Smadl and Smad5, α-Smad3 antibody for Smad3, and α-Smad4 antibody for Smad4 detection (α-Smads blot). Equivalent hSmurfl expression was confirmed as described above (α-Flag blot).

Figures 5 A, 5B, and 5C. hSmurfl regulates Smadl turnover and ubiquitination: hSmurfl enhances Smadl turnover.

(A) COS-1 cells were transiently transfected with pCMV5-Smadl alone or with Flag-tagged hSmurfl (F/hSmurfl) using lipofectAMINE. Two days later, transfectants were subjected to pulse-chase analysis using [³⁵S]methionine. At the indicated time during the chase, cells were lysed and subjected to an α-Smadl immunoprecipitation. The immunoprecipitates were resolved by SDS-PAGE and visualized by autoradiography (left panel). Radiolabelled Smadl was also quantitated by phosphoimaging, and the results are plotted as the amount of [³⁵S] methionine-labelled Smadl present at each time point relative to the level at time 0 (right panel).

(B) Ubiquitination of Smadl in 293T cells. Cells were transiently transfected with indicated combinations of HA-tagged ubiquitin (HA-Ub), pCMV5-Smadl and either wild type (WT) or the ubiquitin ligase mutant (C A) of Flag-tagged hSmurfl . Two days post-transfection, lysates were subjected to an α-Smadl immunoprecipitation (α-Smadl IP) followed by SDS-PAGE and immunoblotting with an α-HA monoclonal antibody (α-HA blot). Protein bands displaying immunoreactivity to α-HA are marked by the square bracket. Expression of Smadl or Flag-hSmurfl was confirmed by subjecting total cell lysates to immunoblotting with an α-Smadl polyclonal antibody (α-Smadl blot) or an α-Flag monoclonal antibody (α-Flag blot), respectively.

(C) Loss in Smadl steady-state level by hSmurfl requires an intact ubiquitin ligase activity of the Hect domain. 293T cells were transfected with Smadl and increasing amounts of the wild type (WT) or the ubiquitin ligase mutant (C710A). Total cell lysates were analyzed for Smadl, hSmurfl or hSmurfl (C710 A) protein by immunoblotting total cell lysates with the appropriate antibody, as described in Fig 3. Figures 6A, 6B, and 6C. Interaction of Smurfl and Smads. (A) Smurfl interacts with Smadl . Yeast two-hybrid assays were done on yeast co-transformed with combinations of Xenopus Smurfl (Xsmurf) together with Smadl, Smad2, lamin or vector alone. Only the combination of Smadl and Smurfl exhibited significant β-galactosidase activity (left panel, photograph of stained yeast colonies assayed in duplicate). Co-immunoprecipitation on in vitro translated proteins was done by incubating 35S-labelled Smurfl together with in vitro translated, 35S-Met trace-labelled Flag-tagged Smadl (35S-F/Smadl), Smad2 (35S-F/Smad2) or Smad4 (35S-F/Smad4) immobilized on anti-Flag affinity gel matrices. After washing, bound proteins were eluted and analyzed by SDS-PAGE (right panel). (B) hSmurfl interacts selectively with both Smadl and Smad5. 293T cells were transiently transfected with the pCMV5 expression vectors containing Smadl (SI), Smad5 (S5) or Smad2 either alone, with wild type (WT) or the ubiquitin ligase mutant (CA) Flag-tagged hSmurfl (F/hSmurfl). To examine Smadl or Smad5 interaction with hSmurfl, blots of α-Flag immunoprecipitates were probed with α-Smadl polyclonal antibody (α-Smadl blot). To examine Smad2 interaction with hSmurfl , blots of α-Flag immunoprecipitates were probed with α-Smad2 polyclonal antibody (α-Smad2 blot). The level of hSmurfl in the immunoprecipitates and Smadl, Smad5 and Smad2 in the total cell lysates, are shown in the lower two panels.

(C) Specificity of hSmurfl actions on Smadl . Nedd4, a ubiquitin ligase related to hSmurfl , does not interact with and does not reduce the steady-state level of Smadl . 293T cells were transiently co-transfected with Smadl and either Flag-tagged hSmurfl (WT or CA) or Nedd4, (WT) or ligase mutant (CS) as indicated. Smadl coimmunprecipitation with hSmurfl (left panels) was determined as described above. To determine Smadl interaction with Nedd4, cell lysates were subjected to immunoprecipitation using a-Hect-Nedd4 polyclonal antibody (α-Nedd4 IP) followed by immunoblotting with α-Smadl polyclonal antibody (α-Smadl blot), right panels. To determine the level of expression of Nedd4 in the samples, the respective blots were reprobed with a-WW2 Nedd4 polyclonal antibody (α-Nedd4 blot). Smadl levels in total cell lysates of these assays are shown by western blot in the bottom panel. Figures 7A and 7B. Smurfl dorsalizes prospective ventral mesoderm and neuralizes ectoderm.

(A) Four cell Xenopus blastulae were injected with Xenopus Smurf mRNA in the marginal zone of two ventral blastomeres (50 pg Smurfl mRNA per cell). Tadpoles that developed from injected embryos formed ectopic, dorsal axial structures (lower left panel, arrows). Co-injection of 100 pg Smadl together with 50 pg of Smurfl mRNA rescued the ectopic axial structures in all cases (lower tadpole). At early gastrulation ventral marginal zone (VMZ) pieces were explanted from another set of wild-type embryos, or embryos injected in the VMZ at the four cell stage with SmurflmRNA alone, or Smurfl co-injected with Smadl. The VMZ explants were excised at early gastrulation, as depicted, then cultured until control embryos reached mid-tadpole, stage 28, when total VMZ RNA was prepared then assayed by RT-PCR for the expression of erythrocyte-specific α-globin, muscle-specific actin, and general cellular mRNA, eFl-a, as a control for RNA recovery (lower right panel). Control PCR reactions were done on total embryonic RNA with or without reverse-transcription.

(B) Fertilized egg animal poles were injected with Smurfl and/or Smadl mRNA, or were not injected. Animal caps were explanted at blastula stage 8, cultured to mid-gastrula stage 11 , then total RNA was prepared and assayed by RT-PCR to detect expression of the cement gland marker, XAG, and a general neural marker, NCAM. Controls were as above.

Figures 8A, 8B, and 8C. Smurfl alters embryonic cell competence to respond to Smadl and Smad2.

(A) Smurfl blocks ventral mesoderm induction by Smadl. A constant amount (1 ng) of Smadl mRNA was injected alone, or together with an increasing amount of Smurfl . into fertilized egg animal poles and ventral mesoderm induction in animal cap explants was assayed by RT-PCR with primers for the Xhox3 and Xcad3 homeodomain genes. In lanes from left to right, respectively, animal caps were not injected or injected with Smurfl mRNA at doses of 100, 0, 25, 50, 100, and 200 pg.

(B) Smurfl enhances dorsal mesoderm induction by Smad2. A constant amount (50 pg) of Smad2 mRNA was injected alone, or together with an increasing amount of Smurfl, and dorsal mesoderm induction was monitored by expression of myoD, which marks muscle, and goosecoid, a homeodomain gene expressed in the most dorsal type of mesoderm, the Spemann Organizer. Note that goosecoid expression was triggered from undetectable levels, as the dose of Smurfl was increased. Animal caps were injected as in panel a.

(C) Dose-response of animal caps to Smad2. Animal caps were injected with synthetic mRNAs for Smad2. At 50 pg Smad2 MyoD was induced and reached maximal levels at 100 pg or more injected Smad2. Goosecoid was induced at a minimum Smad2 dose of 250 pg. The level of goosecoid induced by 250 pg Smad2 alone was equivalent to the level of goosecoid induced by a combination of 50 pg of Smad2 and 100 pg of Smurfl (panel b). In all panels the far right two lanes correspond to PCR on wildtype embryonic RNA, with (RT+) and without (RT-) reverse transcription, respectively. eFl-a controls in all panels were as in Fig. 7.

Figure 9. cDNA sequence of human Smurfl [SEQ ID NO: 1].

Figure 10. Protein sequence of human Smurfl [SEQ ID NO:2]. Figure 11. cDNA sequence of human Smurf2 [SEQ ID NO: 3].

Figure 12. Protein sequence of human Smurfl [SEQ ID NO: 4]. SmurfZ is member of the HECT E3 ubiquitin-ligase family. The C2 (overline), WW (shaded) and HECT (double overline) domains and the Cys716Ala mutation (boxed) are shown.

Figure 13. Comparison of Smurfl and Smurfl proteins . Schematic representation of Smurfl and SmurfZ. The degree of amino acid identity (%) between the C2, WW and HECT domains is shown.

Figures 14A and 14B. Smurfl is expressed in mouse tissues.

(A) SmurfZ is expressed throughout mouse embryo genesis. A 1 kb Xhol/Notl fragment encompassing the 3'UTR of mouse SmurfZ was used to probe a mouse embryonic Northern Blot (Clontech).

(B) Expression of Smurf2 in adult mouse tissues. A multiple tissue northern blot (Clontech) was probed with a fragment of mouse SmurfZ as in A.

Figures 15A, 15B, 15C, 15D, 15E and 15F. Smurfl interacts with Smadl. (A and B) Expression of SmurfZ does not decrease steady-state levels of the Smads. 293T cells were transfected with Flag-tagged Smadl, Smad2, Smad4 or HA- tagged Smad7 either alone or together with the Myc -tagged Smurf2. Aliquots of total cell lysates were immunoblotted to detect expression of SmurfZ and the Smads (Fig. 15 A) or were subjected to immunoprecipitation with anti-Myc antibody followed by anti-Flag or anti-HA immunoblotting to detect any coprecipitating Smads (Fig. 15B). The migration of the anti-Myc heavy chain (IgH) is marked.

(C) Expression of SmurfZ does not alter Smad7 turnover. COS-1 cells, transfected with either Smad7-HA alone or together with Flag-SmurfZ, were pulse-labelled with [³⁵S]-methionine and then chased for the indicated times in media containing unlabelled methionine. [³⁵S]-labelled Smad7-HA in anti-HA immunoprecipitates was quantified by phosphorimaging and the levels in control cells (squares) and Smurf2 expressing cells (circles) was plotted relative to the amount present at time 0. Data represents the average of two experiments +/- SD.

(D) In vitro interaction of bacterially-expressed SmurfZ and Smad7. Bacterially-produced His-Smad7-HA protein was incubated with Ni²⁺-NTA, GST and GST-Smurf2. Bound material was visualized by SDS-PAGE and immunoblotting with anti-HA antibody. Levels of GST proteins were determined by Coomassie staining (bottom panel).

(E) The PY motif in Smad7 is an important determinant for mediating interaction with SmurfZ. 293T cells were transfected with Flag-SmurfZ either alone or together with wild type (WT) or mutant Y211A (YA) or ΔPYversions of Smad7-HA. Cell lysates were subjected to anti-Flag immunoprecipitation and coprecipitating Smad7 proteins were detected by immunoblotting with anti-Smad7 antibody. Smad7 expression was confirmed by immunoblotting aliquots of total cell lysates (bottom panel).

(F) The WW domains of Smurf2 are necessary for binding to Smad7. 293T cells were transfected with Smad7-HA and either wild type (WT) or mutant (ΔWW1, ΔWW2 or ΔWW3) versions of Flag-SmurfZ. Cell lysates were subjected to anti-Flag immunoprecipitation, and coprecipitating Smad7 was detected by immunoblotting with anti-HA antibody. Smad7 expression was confirmed by immunoblotting aliquots of total cell lysates (bottom panel).

Figure 16. Smad? recruits Smurfl to the TGFβ receptor complex. COS-1 cells were transfected with various combinations of TβRII, TβRI-

HA, Smad7-HA and wild type (WT) or mutant (C716A) Flag-Smurf2 as indicated. Cells were affinity-labelled with [¹²T]TGFβand lysates immunoprecipitated with anti-Flag or anti-Smad7 antibodies. Coprecipitating receptor complexes were visualized by SDS- PAGE and autoradiography. The amount of coprecipitating TβRI was quantified by phosphorimaging (right panels). Receptor expression was confirmed by visualizing aliquots of total cell lysates by autoradiography. Smurf2 and Smad7 levels were confirmed by immunoblotting aliquots of total cell lysates with anti-Flag and anti-HA antibodies, respectively.

Figures 17A, 17B, 17C, 17D and 17E. Smurfl induces degradation of TGFβreceptors and Smadl.

(A) Smurfl expression in the absence of Smadl does not decrease receptor steady-state levels. 293T cells were transfected with various combinations of TβRII-HA, TβRI-HA and varying amounts of Flag-SmurfZ (plasmid DNA in micrograms) is indicated. Expression levels of proteins were determined by immunoblotting aliquots of total cell lysates using the appropriate antibodies as shown.

(B) Smurfl in the presence of Smadl causes a decrease in steady-state receptor levels. 293 T cells were transfected with Smad7-HA, either TβRII-HA and TβRI- HA (left panels) or with a constitutively active type I receptor, TβRI-HA (T204D) (right panels) together with increasing amounts of wild type (WT) or mutant Flag-Smurf2 (C716A). Steady-state levels of the receptors, Smad7 and SmurfZ were determined by anti-HA or anti-Flag immunoblotting as indicated.

(C) Smurfl increases the turnover rate of the receptor complex. COS-1 cells transfected with TGFβreceptors (TβRII-HA and TβRI-HA) alone or together with Smad7-HA, Flag-SmurfZ or both were pulse-labelled with [³⁵S] -methionine and then chased for the indicated times in media containing unlabelled methionine. Cell lysates were subjected to anti-HA immunoprecipitation and the amount of labelled receptors and Smad7 was quantified by phosphorimaging and is plotted relative to the amount present at time 0.

(D) Proteasome and lysosome inhibitors block Smurfl-induced degradation of the receptor complex. COS-1 cells transfected with TGFβ receptors (TβRII-HA and TβRI-HA), Smad7-HA and Flag-SmurfZ were pulse-labelled with [³⁵S] -methionine and then chased either in absence of inhibitors or in the presence of 30mM lactacystin, or 0.4 mM chloroquine for the indicated times. Cell lysates were subjected to anti-HA immunoprecipitation and receptor and Smad7 levels were visualized by SDS-PAGE and autoradiography. (E) Smurfl induces the ubiquitination of Smad? in the presence of the receptors. 293T cells were transfected with HA-tagged ubiquitin together with various combinations of Smad7, TβRII, TβRI-Flag, and wild type (WT) or mutant (C716A) Myc- SmurfZ as indicated. Cell lysates were subjected to a double immunoprecipitation with anti-Smad7 antibodies followed by immunoblotting with anti-HA antibodies. Protein expression in aliquots of total cell lysates was confirmed by immunblotting.

Figures 18A, 18B, 18C and 18D. Association of Smurfl with Smadl enhances the Smad? inhibitory activity.

(A) Smad7(Y21lA binds to TGFβ receptors but has a reduced ability to recruit Smurfl to the receptor complex. COS-1 cells were transfected with TGFβ receptors (TβRII and TβRI-HA) and either wild type (WT) or mutant ( Y211 A) Smad7/HA in the absence or presence of Flag-SmurfZ(C716A). Cells were affinity-labelled with [¹²⁵I]TGFβ and lysates immunoprecipitated with anti-Smad7 or anti-Flag antibodies. Coprecipitating receptor complexes were visualized by SDS-PAGE and autoradiography. Total receptor expression was determined by autoradiography and Smad7 and SmurfZ protein levels were confirmed by anti-HA or anti-Flag immunoblotting of aliquots of total cell lysates.

(B and C) Smad7(Y211A) is not as effective as wild type Smad? in inhibiting TGFb-dependent activation of transcription. HepG2 cells were transfected with the 3TP-Lux reporter alone or together with varying concentrations of wild type (WT) or mutant (Y21 1A) Smad7-HA. In (B), 0.3 ng/ml of each Smad7 plasmid was used. Cells were incubated in the presence or absence of TGFβ 1, and luciferase activity was normalized to b-galactosidase activity and is plotted as the mean +/- SD of triplicates from representative experiments.

(D) A model for Smadl and Smurfl-mediated degradation of the TGFβ receptor complex. Smad7 binds directly to SmurfZ and associates with the TGFβ receptor complex. Thus, Smad7 functions as an adaptor protein that mediates degradation of the TGFβ receptor complex.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is directed to a family of genes encoding E3 ubiquitin ligases, called Smurf proteins, and includes full length, or naturally occurring forms, and any functionally active or antigenic fragments thereof, from any animal, particularly mammal or amphibian, and more particularly from a human source. In specific embodiments, the E3 ubiquitin ligases called Smurfl and SmurfZ are characterized.

This invention is based, in part, on the suφrising discovery that two novel members of the Hect family of ubiquitin ligases interact with Smads. These ligases have been named Smurfl and Smurf2. One of these, Smurfl, specifically targets BMP pathway-specific Smads, thereby acting as an antagonist or negative regulator of BMP signaling. Smurfl directly interacts with Smadsl and 5 and regulates their ubiquitination, turnover, and activity. In amphibian embryos, Smurfl inhibits endogenous BMP signals, resulting in altered pattern formation and cell fate in the mesoderm and ectoderm. Thus, the invention provides a unique regulatory protein link between the ubiquitination pathway and the control of cell fate, e.g., during embryonic development. The invention further provides a nucleic acid encoding Smurfl [SEQ ID NO: 1], in addition to the Smurfl protein, [SEQ ID NO: 2], and mutant variants thereof. The other novel member of the Hect family of ubiquitin ligases is SmurfZ.

SmurfZ, a C2-WW-HECT domain E3 ubiquitin ligase is related to Smurfl . SmurfZ does not interact with Smadl, 2 or 4, nor does SmurfZ alter steady state levels of Smad 1, 2 or 4. SmurfZ, however, does interact with Smad 7, binding directly to a PPXY motif in Smad7. Smurf is involved with TGFβ receptor degradation acting in partnership with Smad7 as an antagonist or negative regulator of TGFβ signaling. Activation of TGFβ signalling results in Smad7-dependent recruitment of SmurfZ to the TGFβ receptor complex. In the absence of activated TGFβ receptor complex, SmurfZ does not alter the steady state level and turnover of Smad7. Recruitment of Smurf2 to the TGFβ receptor by Smad7 promotes the degradation of the Smad7- TGFβ receptor complex by both proteasomal and lysosomal pathways. The studies described herein demonstrate that

Smad7 functions as an adaptor protein that recruits SmurfZ to the TGFβ receptor complex to promote its degradation and thereby down-regulate activated TGFβ receptor complexes. Regulation of Smad 7 localization to the nucleus and interaction with SmurfZ may be used to control the inhibitory activity of the Smad 7-Smurf2 complex. The invention further provides a nucleic acid encoding SmurfZ [SEQ ID NO: 3], in addition to the Smurf 2 protein [SEQ ID NO: 4] and variants thereof.

E3 ubiquitin ligases display very selective substrate specificity, as is evident in the findings discussed herein. For example, Smurfl binds BMP pathway-specific Smads. Moreover, Smurfl is a unique signaling protein of the BMP pathway because it binds only to Smadl and Smad5 and has little affinity for Smad 2 (specific for TGFβ and activin receptor pathways), and no affinity for Smad4 (common Smad signaling partner). As a result of this substrate specificity, Smurfl can effectively interfere or regulate biological responses to BMPs without consequence to activin pathways, i.e., effects on other TGFβ signaling pathways are limited, or non-existent.

BMPs/TGFβ signalling pathway functions in tissue differentiation, moφhogenesis, and cell growth control (e.g., (52)). As an antagonist to the signal transduction pathway mediated by the TGFβ family, Smurfl will inhibit the BMP pathway in vivo or in vitro. As an integral component of regulatory system for degradation of TGFβ receptors, Smurf will inhibit the TGFβ signalling pathway. As a result, the Smurfs will block chondrogenesis, osteogenesis, blood differentiation, cartilage formation, neural tube patterning, retinal development, heart induction and moφhogenesis, hair growth, tooth formation, gamete formation and a wide variety of tissue and organ formation processes, and hinder the regeneration, growth, maintenance, etc., of bone and other tissues that are dependent on the BMP pathway.

In one embodiment, mutant forms of a Smurf protein or small molecule antagonists of Smurf, described infra, can be used to prevent ubiquitination of proteins, e.g., Smads or TGFβ receptor, and therefore to preserve the signal transduction pathway mediated by BMPs. In addition, fragments of Smads can be generated that bind to either the Hect domain of a Smurf protein, which domain has the catalytic activity needed for ubiquitination of Smads, or to the WW domains that interact with the PPXY domains of Smads, thus precluding Smurfl from binding and ubiquitinating Smads. These fragments can also be used in screening assays for small molecule inhibitors of Smurf-Smad interaction, e.g., in an inhibition binding assay. Variants of a Smurf protein and fragments of Smads can be used to improve defective BMP and/or TGFβ/activin signaling as a result of overexpressed or increased Smurf activity, which may contribute to a disease state.

BMPs control bone differentiation and growth, and are already in clinical tests and applications related to bone growth and connective tissue repair. Smurf proteins represent a novel target for the discovery of drugs that can influence its function, thereby affecting cellular responses to BMPs, and thus having clinical applications. Therefore, a Smurf protein can be used for screening for various drugs and/or antibodies that can either enhance the BMP pathway, or inhibit it by antagonizing or mimicking the activity of the protein, respectively. For example, since Smurfl is highly specific for binding Smadsl and 5, it can be used to screen for drugs that block or activate the BMP pathway, and selectively affect cellular responses to BMPs without consequence to other members of the TGFβ superfamily.

Smurf proteins operate within cells, at the level of Smad signal transduction, and therefore provide an alternative means to affect BMP and TGFβ/activin signals. However, because Smurf proteins are intracellular proteins, any manipulations aimed at directly altering Smurf activity must operate intracellularly. Such manipulations include antisense and ribozyme technology, and intracellular antibody technology. Smurf may be delivered to cells in gene therapy regimens to block excessive signaling by particular growth factor pathways controlled by Smads, e.g., Smads that are targets for Smurfl or Smurf2. The examples herein show that simply increasing the expression levels of Smurfl in cells antagonizes the Smad signaling pathway. Thus overexpression of Smurfs by gene therapy may be used to correct clinical conditions that result from excessive Smad signaling. These may include, for example, hypeφlasia of bone, tendon or cartilage tissues, or formation of other tissues, that are regulated by signals from receptors (such as BMP receptors) that utilize Smadl or Smad5 for signal transduction. Smurf nucleic acids or partial sequences thereof (such as PCR probes) would be useful as molecular probes for identification of defective Smurf genes in the human genome, particularly where a mutation of a Smurf gene is found in association with a particular disease. Smurf proteins may be used as reagents for in vitro assays to identify proteins in cells that are targets for ubiquitination. Purified Smurfs may be reconstituted with purified ubiquitination enzymes (i.e. El and E2 components) and utilized in functional (ubiquitination) assays that are aimed at identifying novel target proteins introduced into the assays (as purified proteins or translated cDNAs of unknown identity).

In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (herein "Sambrook et al, 1989"); DNA Cloning: A Practical Approach, Volumes I and II (D.N. Glover ed. 1985); Oligonucleotide Synthesis (M.J. Gait ed. 1984); Nucleic Acid Hybridization [B.D. Hames & S.J. Higgins eds. (1985)]; Transcription And Translation [B.D. Hames & S.J. Higgins, eds. (1984)]; Animal Cell Culture [R.I. Freshney, ed. (1986)]; Immobilized Cells And Enzymes [IRL Press, (1986)]; B. Perbal, A Practical Guide To Molecular Cloning (1984); F.M. Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (1994).

If appearing herein, the following terms shall have the definitions set out below.

The term "about" or "approximately" means within 20%, preferably within 10%, and more preferably within 5% of a given value or range.

As used herein, the term "isolated" means that the referenced material is free of components found in the natural environment in which the material is normally found. In particular, isolated biological material is free of cellular components. In the case of nucleic acid molecules, an isolated nucleic acid includes a PCR product, an isolated mRNA, a cDNA, or a restriction fragment. In another embodiment, an isolated nucleic acid is preferably excised from the chromosome in which it may be found, and more preferably is no longer joined to non-regulatory, non-coding regions, or to other genes, located upstream or downstream of the gene contained by the isolated nucleic acid molecule when found in the chromosome. In yet another embodiment, the isolated nucleic acid lacks one or more introns. Isolated nucleic acid molecules can be inserted into plasmids, cosmids, artificial chromosomes, and the like. Thus, in a specific embodiment, a recombinant nucleic acid is an isolated nucleic acid. An isolated protein may be associated with other proteins or nucleic acids, or both, with which it associates in the cell, or with cellular membranes if it is a membrane-associated protein. An isolated organelle, cell, or tissue is removed from the anatomical site in which it is found in an organism. An isolated material may be, but need not be, purified.

The term "purified" as used herein refers to material that has been isolated under conditions that reduce or eliminate unrelated materials, i.e., contaminants. For example, a purified protein is preferably substantially free of other proteins or nucleic acids with which it is associated in a cell; a purified nucleic acid molecule is preferably substantially free of proteins or other unrelated nucleic acid molecules with which it can be found within a cell. As used herein, the term "substantially free" is used operationally, in the context of analytical testing of the material. Preferably, purified material substantially free of contaminants is at least 50% pure; more preferably, at least 90% pure, and more preferably still at least 99% pure. Purity can be evaluated by chromatography, gel electrophoresis, immunoassay, composition analysis, biological assay, and other methods known in the art. A "gene" is used herein to refer to a portion of a DNA molecule that includes a polypeptide coding sequence operatively associated with expression control

Claims

sequences. In one embodiment, a gene can be a genomic or partial genomic sequence, in that it contains one or more introns. In another embodiment, the term gene refers to a cDNA molecule (i.e., the coding sequence lacking introns).A DNA "coding sequence" is a double-stranded DNA sequence which is transcribed and translated into a polypeptide in a cell in vitro or in vivo when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a start codon at the 5 ' (amino) terminus and a translation stop codon at the 3 ' (carboxyl) terminus. A coding sequence can include, but is not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and even synthetic DNA sequences. If the coding sequence is intended for expression in a eukaryotic cell, a polyadenylation signal and transcription termination sequence will usually be located 3 ' to the coding sequence. "Expression control sequences", e.g., transcriptional and translational control sequences, are regulatory sequences that flank a coding sequence, such as promoters, enhancers, suppressors, terminators, and the like, and that provide for the expression of a coding sequence in a host cell. In eukaryotic cells, polyadenylation signals are control sequences. On mRNA, a ribosome binding site is an expression control sequence.A "promoter sequence" is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3 ' direction) coding sequence. For puφoses of defining the present invention, the promoter sequence is bounded at its 3 ' terminus by the transcription initiation site and extends upstream (5 ' direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence will be found a transcription initiation site (conveniently defined for example, by mapping with nuclease SI), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase.A coding sequence is "under the control" of transcriptional and translational control sequences in a cell when RNA polymerase transcribes the coding sequence into mRNA, which is then trans-RNA spliced (if it contains introns) and translated into the protein encoded by the coding sequence.A nucleic acid molecule is "hybridizable" to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid molecule can anneal to the other nucleic acid molecule under the appropriate conditions of temperature and solution ionic strength (see Sambrook et al, supra). The conditions of temperature and ionic strength determine the "stringency" of the hybridization. For preliminary screening for homologous nucleic acids, low stringency hybridization conditions, corresponding to a Tm of 55 °C, can be used, e.g., 5x SSC, 0.1% SDS, 0.25% milk, and no formamide; or 30% formamide, 5x SSC, 0.5% SDS). Moderate stringency hybridization conditions correspond to a higher Tm, e.g., 40%) formamide, with 5x or 6x SCC. High stringency hybridization conditions correspond to the highest Tm, e.g., 50% formamide, 5x or 6x SCC. Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of Tm for hybrids of nucleic acids having those sequences. The relative stability (corresponding to higher Tm) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (see Sambrook et al, supra, 9.50-9.51). For hybridization with shorter nucleic acids, i.e., oligonucleotides, the position of mismatches becomes more important, and the length of the oligonucleotide determines its specificity (see Sambrook et al, supra, 11.7-11.8). A minimum length for a hybridizable nucleic acid is at least about 10 nucleotides; preferably at least about 15 nucleotides; and more preferably the length is at least about 20 nucleotides.In a specific embodiment, the term "standard hybridization conditions" refers to a Tm of 55 °C, and utilizes conditions as set forth above. In a preferred embodiment, the Tm is 60 °C; in a more preferred embodiment, the Tm is 65 °C. In a specific embodiment, "high stringency" refers to hybridization and/or washing conditions at 68 °C in 0.2XSSC, at 42 °C in 50% formamide, 4XSSC, or under conditions that afford levels of hybridization equivalent to those observed under either of these two conditions.A "vector" is a recombinant nucleic acid construct, such as plasmid, phage genome, virus genome, cosmid, or artificial chromosome to which another DNA segment may be attached. In a specific embodiment, the vector may bring about the replication of the attached segment, e.g., in the case of a cloning vector. A segment of DNA is inserted into a vector at specific restriction sites. The segment of DNA encodes a polypeptide of interest and the segment and restriction sites are designed to ensure insertion of the segment in the proper reading frame for transcription and translation.A cell has been "transfected" by exogenous or heterologous DNA when such DNA has been introduced inside the cell. A cell has been "transformed" by exogenous or heterologous DNA when the transfected DNA effects a phenotypic change. Preferably, the transforming DNA should be integrated (covalently linked) into chromosomal DNA making up the genome of the cell.The term "heterologous" refers to a combination of elements not naturally occurring. For example, heterologous DNA refers to DNA not naturally located in the cell, or in a chromosomal site of the cell. Preferably, the heterologous DNA includes a gene foreign to the cell. A heterologous expression regulatory element is such an element operatively associated with a different gene than the one it is operatively associated with in nature. In the context of the present invention, a Smurfl or SmurfZ gene is heterologous to the plasmid vector DNA in which it is inserted for cloning or expression, and it is heterologous to a non-human host cell in which it is expressed, e.g. , a CHO cell.A "nucleic acid molecule" refers to the phosphate ester polymeric form of ribonucleosides (adenosine, guanosine, uridine or cytidine; "RNA molecules") or deoxyribonucleosides (deoxyadenosine, deoxyguanosine, deoxythymidine, or deoxy cytidine; "DNA molecules"), or any phosphoester anologs thereof, such as phosphorothioates and thioesters, in either single stranded form, or a double-stranded helix. Double stranded DNA-DNA, DNA-RNA and RNA-RNA helices are possible. The term nucleic acid molecule, and in particular DNA or RNA molecule, refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inter alia, in linear or circular DNA molecules (e.g. , restriction fragments), plasmids, and chromosomes. In discussing the structure of particular double-stranded DNA molecules, sequences may be described herein according to the normal convention of giving only the sequence in the 5 ' to 3 ' direction along the nontranscribed strand of DNA (/. e. , the strand having a sequence homologous to the mRNA). A "recombinant DNA molecule" is a DNA molecule that has undergone a molecular biological manipulation.The invention provides antisense nucleic acids (including ribozymes), which may be used to inhibit expression of Smurfl , particularly to enhance the BMP pathway via Smadsl and 4. Thus, antisense nucleic acids corresponding to the Smurfl gene, or a fragment thereof, can be used to alter BMP pathways. The invention also provides antisense nucleic acids to inhibit expression of SmurfZ, to enhance the TGFβ signalling pathway. An "antisense nucleic acid" is a single stranded nucleic acid molecule which, upon hybridizing with complementary bases in an RNA or DNA molecule, inhibits the latter's role. If the RNA is a messenger RNA transcript, the antisense nucleic acid is a countertranscript or mRNA-interfering complementary nucleic acid. As presently used, "antisense" broadly includes RNA-RNA interactions, RNA-DNA interactions, ribozymes and RNase-H mediated arrest. Antisense nucleic acid molecules can be encoded by a recombinant gene for expression in a cell (e.g., U.S. Patent No. 5,814,500; U.S. Patent No. 5,811,234), or alternatively they can be prepared synthetically (e.g., U.S. Patent No. 5,780,607).As used herein, the term "oligonucleotide" refers to a nucleic acid, generally of at least 10, preferably at least 15, and more preferably at least 20 nucleotides, that is hybridizable to a genomic DNA molecule, a cDNA molecule, or an mRNA molecule encoding a gene, mRNA, cDNA, or other nucleic acid of interest. Oligonucleotides can be labeled, e.g., with 32P-nucleotides or nucleotides to which a label, such as biotin, has been covalently conjugated. In one embodiment, a labeled oligonucleotide can be used as a probe to detect the presence of a nucleic acid. In another embodiment, oligonucleotides (one or both of which may be labeled) can be used as PCR primers, either for cloning full length or a fragment of Smurfl or SmurfZ, or to detect the presence of nucleic acids encoding Smurfl or SmurfZ. In a further embodiment, an oligonucleotide of the invention can form a triple helix with a Smurfl or SmurfZ DNA molecule. In still another embodiment, a library of oligonucleotides arranged on a solid support, such as a silicon wafer or chip, can be used to detect various polymoφhisms of interest. Generally, oligonucleotides are prepared synthetically, preferably on a nucleic acid synthesizer. Accordingly, oligonucleotides can be prepared with non-naturally occurring phosphoester analog bonds, such as thioester bonds, etc. Specific examples of synthetic oligonucleotides envisioned for this invention include oligonucleotides that contain phosphorothioates, phosphotriesters, methyl phosphonates, short chain alkyl, or cycloalkl intersugar linkages or short chain heteroatomic or heterocyclic intersugar linkages. Most preferred are those with CH2-NH- O-CH2, CH2-N(CH3)-O-CH2, CH2-O-N(CH3)-CH2, CH2-N(CH3)-N(CH3)-CH2 and O- N(CH3)-CH2-CH2 backbones (where phosphodiester is O-PO2-O-CH2). US Patent No. 5,677,437 describes heteroaromatic olignucleoside linkages. Nitrogen linkers or groups containing nitrogen can also be used to prepare oligonucleotide mimics (U.S. Patents No. 5,792,844 and No. 5,783,682). US Patent No. 5,637,684 describes phosphoramidate and phosphorothioamidate oligomeric compounds. Also envisioned are oligonucleotides having moφholino backbone structures (U.S. Pat. No. 5,034,506). In other embodiments, such as the peptide-nucleic acid (PNA) backbone, the phosphodiester backbone of the oligonucleotide may be replaced with a polyamide backbone, the bases being bound directly or indirectly to the aza nitrogen atoms of the polyamide backbone (Nielsen et al, Science 254:1497, 1991). Other synthetic oligonucleotides may contain substituted sugar moieties comprising one of the following at the 2' position: OH, SH, SCH3, F, OCN, O(CH2)nNH2 or O(CH2)nCH3 where n is from 1 to about 10; C, to C10 lower alkyl, substituted lower alkyl, alkaryl or aralkyl; Cl; Br; CN; CF3; OCF3; O-; S-, or N-alkyl; O-, S-, or N-alkenyl; SOCH3 ; SO2CH3; ONO2;NO2; N3; NH2; heterocycloalkyl; heterocycloalkaryl; aminoalkylamino; polyalkylamino; substitued silyl; a fluorescein moiety; an RNA cleaving group; a reporter group; an intercalator; a group for improving the pharmacokinetic properties of an oligonucleotide; or a group for improving the pharmacodynamic properties of an oligonucleotide, and other substituents having similar properties. Oligonucleotides may also have sugar mimetics such as cyclobutyls or other carbocyclics in place of the pentofuranosyl group. Nucleotide units having nucleosides other than adenosine, cytidine, guanosine, thymidine and uridine may be used, such as inosine.As used herein, the term "homologous" in all its grammatical forms and spelling variations refers to the relationship between proteins that possess a "common evolutionary origin," including proteins from superfamilies (e.g., the TGFβ superfamily) and homologous proteins from different species (e.g., Smad (human), Mad (drosophila), etc.) (Reeck et al, Cell 50:667, 1987). Such proteins, and their encoding genes, have sequence homology, as reflected by their high degree of sequence similarity.Accordingly, the term "sequence similarity" in all its grammatical forms refers to the degree of identity or correspondence between nucleic acid or amino acid sequences of proteins that may or may not share a common evolutionary origin (see Reeck et al, supra). However, in common usage and in the instant application, the term"homologous," when modified with an adverb such as "highly," may refer to sequence similarity and may or may not relate to a common evolutionary origin.In a specific embodiment, two DNA sequences are "substantially homologous" or "substantially similar" when at least about 70-75%, and most preferably at least about 80-85% of the nucleotides match over the defined length of the DNA sequences. An example of such a sequence is an allelic or species variant of a Smurf gene of the invention. Sequences that are substantially homologous can be identified by comparing the sequences using standard software available in sequence data banks, or in a Southern hybridization experiment under, for example, stringent conditions as defined for that particular system. Defining appropriate hybridization conditions is within the skill of the art. See, e.g., Maniatis et al, supra; DNA Cloning, Vols. I & II, supra; Nucleic Acid Hybridization, supra.Similarly, in a particular embodiment, two amino acid sequences are "substantially homologous" or "substantially similar" when greater than 70% of the amino acids are identical, or greater than about 90% are similar (functionally similar). Preferably, the similar or homologous sequences are identified by alignment using, for example, the GCG (Genetics Computer Group. Program Manual for the GCG Package, Version 1, Madison, Wisconsin) pileup program, BLAST, and Clustal W analysis (MacVector). Sequence comparison algorithms can also be found at http://www.nwfsc.noaa.gov/bioinformatics.html. The term "corresponding to" is used herein to refer similar or homologous sequences, whether the exact position is identical or different from the molecule to which the similarity or homology is measured. A nucleic acid or amino acid sequence alignment may include spaces. Thus, the term "corresponding to" refers to the sequence similarity, and not the numbering of the amino acid residues or nucleotide bases. "Homologous recombination" refers to the insertion of a foreign DNA sequence of a vector in a chromosome. Preferably, the vector targets a specific chromosomal site for homologous recombination. For specific homologous recombination, the vector will contain sufficiently long regions of homology to sequences of the chromosome to allow complementary binding and incoφoration of the vector into the chromosome. Longer regions of homology, and greater degrees of sequence similarity, may increase the efficiency of homologous recombination.A DNA "coding sequence" is a double-stranded DNA sequence which is transcribed and translated into a polypeptide in a cell in vitro or in vivo when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a start codon at the 5 ' (amino) terminus and a translation stop codon at the 3 ' (carboxyl) terminus. A coding sequence can include, but is not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and even synthetic DNA sequences. If the coding sequence is intended for expression in a eukaryotic cell, a polyadenylation signal and transcription termination sequence will usually be located 3 ' to the coding sequence. Transcriptional and translational control sequences are DNA regulatory sequences, such as promoters, enhancers, terminators, and the like, that provide for the expression of a coding sequence in a host cell. In eukaryotic cells, polyadenylation signals are control sequences. A "promoter sequence" is a DNA regulatory region capable of bindingRNA polymerase in a cell and initiating transcription of a downstream (3 ' direction) coding sequence. For puφoses of defining the present invention, the promoter sequence is bounded at its 3 ' terminus by the transcription initiation site and extends upstream (5 ' direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence will be found a transcription initiation site (conveniently defined for example, by mapping with nuclease SI), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase.A coding sequence is "under the control" of transcriptional and translational control sequences in a cell when RNA polymerase transcribes the coding sequence into mRNA, which is then trans-RNA spliced and translated into the protein encoded by the coding sequence.A nucleic acid coding a protein of the Smurf family, e.g., Smurfl or SmurfZ, whether genomic DNA or cDNA, can be isolated from any source, particularly from a human or xenopus cDNA or genomic library. Methods for obtaining genes are well known in the art, as described above (see, e.g., Sambrook et al, 1989, supra). In a specific embodiment, the invention provides cDNA sequences for human Smurfl (hSmurfl) and Smurf2 (hSmurf2) genes [SEQ ID NO: 1 and SEQ ID NO: 3].Accordingly, any animal cell can potentially serve as the nucleic acid source for the molecular cloning of a Smurf gene. The DNA may be obtained by standard procedures known in the art from cloned DNA (e.g., a DNA "library"), including EST libraries and cDNA libraries prepared from tissues with high level expression of the protein (e.g., a Xenopus Stage 9 (blastula) cDNA library-these are the cells that evidence the highest levels of expression of Smurfl). Other cell lines that may express Smurfl or SmurfZ are frog blastula and gastula ectoderm, mesoderm, and endoderm; mouse embryonic stem cells; and various mammalian cells, such as NIH3T3, PC12, 293T, Hela, and COS. DNA encoding a Smurf protein can also be obtained by chemical synthesis, by cDNA cloning, or by the cloning of genomic DNA, or fragments thereof, purified from the desired cell (See, for example, Sambrook et al, 1989, supra; Glover, D.M. (ed.), 1985, DNA Cloning: A Practical Approach, MRL Press, Ltd., Oxford, U.K. Vol. I, II). Clones derived from genomic DNA may contain regulatory and intron DNA regions in addition to coding regions; clones derived from cDNA will not contain intron sequences. Whatever the source, the gene should be molecularly cloned into a suitable vector for propagation of the gene.Identification of a specific DNA fragment containing a desired Smurf gene can be accomplished in various ways known in the art. For example, a portion of a Smurf gene exemplified below can be purified and labeled to prepare a labeled probe, and the generated DNA may be screened by nucleic acid hybridization to the labeled probe (Benton and Davis, Science 196: 180, 1977; Grunstein and Hogness, Proc. Natl. Acad. Sci. U.S.A. 72:3961, 1975). Those DNA fragments with substantial homology to the probe, such as an allelic variant from another individual, will hybridize. In a specific embodiment, high stringency hybridization conditions are used to identify a homologous Smurfl or SmurfZ gene.The present invention also relates to cloning vectors containing genes encoding analogs and derivatives of a Smurf gene of the invention, e.g., Smurfl or SmurfZ, that have similar or homologous functional activity. The production and use of derivatives and analogs related to Smurfl and Smurf are within the scope of the present invention. For example, a truncated form of Smurfl or SmurfZ can be provided. Such a truncated form includes Smurfl or Smurf2 with a deletion. In a specific embodiment, the derivative or analog is functionally active, i. e. , capable of exhibiting one or more functional activities associated with a full-length, wild-type Smurfl or SmurfZ of the invention. Such functions include binding to a Smad protein, e.g., Smadl, Smad5 or Smad7, and catalyzing the ubiquitination of the bound Smad, of the activated TGFβ receptor/Smad complex, or another protein in a TGFβ/activin pathway. In another embodiment, the fragment has binding affinity but lacks or has reduced catalytic capacity. A Smurf derivative can be made by altering encoding nucleic acid sequences by substitutions, additions or deletions that provide for functionally equivalent molecules. Alternatively, non- functional mutant forms of the Smurf proteins, that may for example compete with the wild-type Smurf protein in the BMP pathway, but which are less effective in ubiquitination of Smads, can be prepared for use in treating disorders associated with compromised BMP signaling pathways as described above. In a specific embodiment, infra, the mutation is C710A, described further in the Examples. In another specific embodiment, with respect to Smurf 2, the mutation is C716A.Further selection can be carried out on the basis of the properties of the gene, e.g., if the gene encodes a protein product having the isoelectric, electrophoretic, amino acid composition, partial or complete amino acid sequence, antibody binding activity, or ligand binding profile of a Smurf protein as disclosed herein. Thus, the presence of the gene may be detected by assays based on the physical, chemical, immunological, or functional properties of its expressed product. For example, cDNA clones, or DNA clones which hybrid-select the proper mRNAs, can be selected which produce a protein that, e.g. , has similar or identical electrophoretic migration, isoelectric focusing or non-equilibrium pH gel electrophores is behavior, proteolytic digestion maps, or antigenic properties as determined for Smurfl, or SmurfZ.The present invention also relates to analogs, and derivatives of a Smurf protein, homologs from other species, and mutant variants, which have the same or a homologous functional activity. The production and use of derivatives, analogs, and mutant variants related to a Smurf protein are within the scope of this invention. In a specific embodiment, the derivative or analog is functionally active, i.e., capable of exhibiting one or more functional activities associated with a full-length, wild-type Smurfl or SmurfZ protein of the invention.Smurf derivatives can be made by altering nucleic acid sequences by substitutions, additions or deletions that provide for functionally equivalent molecules. Preferably, derivatives are made that have enhanced functional activity relative to a native Smurf, e.g., Smurfl or Smurf , or that lack a functional activity, such as catalytic activity in the Hect ubiquitin-ligase domain in the C-terminal portion of the Smurf proteins. In one specific embodiment, mutant hSmurfl having a point mutant at C710A is provided which disrupts the catalytic activity of the Hect domain thereby precluding ubiquitination of Smadsl and 4. In another specific embodiment, mutant hSmurfZ having a point mutation at C716A is provided which disrupts the catalytic activity of the Hect domain, precluding proteolytic degradation of the TGFβ receptor-Smad 7 complex. Alternatively, Smurf protein derivatives may encode soluble fragments of a Smurf protein domain, e.g., WW domain, that have the same or greater affinity for the natural ligands, e.g. Smadsl, 5 or 7. Such soluble derivatives may be potent inhibitors of ligand (i.e., Smadsl, 5 or 7) binding to the Smurf proteins.In another specific embodiment, derivatives or fragments of Smadsl and 4 can be made that bind Smurfl and preclude the E3 ligase from further binding cellular Smad and preventing its ubiquitination. In another embodiment, derivatives or fragments of Smad 7 can be made that reduce the association of SmurfZ to both Smad 7 and the TGFβ receptor. Thus, in one embodiment the invention contemplates use of peptides containing the linker region of R-Smads having a PPXY sequence, which is a conserved motif recognized by WW domains (see, Rotin, Curr. Topics Microbiol. Immunol., 228:115-133, 1998 and (33)) such as those found in Smurfl or SmurfZ, and corresponding nucleic acid sequences.Due to the degeneracy of nucleotide coding sequences, other DNA sequences which encode substantially the same amino acid sequence as a Smurf gene may be used in the practice of the present invention. These include but are not limited to allelic genes, homologous genes from other species, and nucleotide sequences comprising all or portions of Smurf genes which are altered by the substitution of different codons that encode the same amino acid residue within the sequence, thus producing a silent change. Likewise, Smurf derivatives of the invention include, but are not limited to, those containing, as a primary amino acid sequence, all or part of the amino acid sequence of a Smurf protein, including altered sequences in which functionally equivalent amino acid residues are substituted for residues within the sequence resulting in a conservative amino acid substitution. For example, one or more amino acid residues within the sequence can be substituted by another amino acid of a similar polarity, which acts as a functional equivalent, resulting in a silent alteration. Substitutes for an amino acid within the sequence may be selected from other members of the class to which the amino acid belongs. For example, the nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan and methionine. Amino acids containing aromatic ring structures are phenylalanine, tryptophan, and tyrosine. The polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine. asparagine, and glutamine. The positively charged (basic) amino acids include arginine, lysine and histidine. The negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Such alterations will not be expected to affect apparent molecular weight as determined by polyacrylamide gel electrophoresis, or isoelectric point. Particularly preferred substitutions include:- Lys for Arg and vice versa such that a positive charge may be maintained; - Glu for Asp and vice versa such that a negative charge may be maintained;- Ser for Thr such that a free -OH can be maintained; and- Gin for Asn such that a free CONH2 can be maintained.Amino acid substitutions may also be introduced to substitute an amino acid with a particularly preferable property. For example, a Cys may be introduced a potential site for disulfide bridges with another Cys. A His may be introduced as a particularly "catalytic" site (i.e., His can act as an acid or base and is the most common amino acid in biochemical catalysis). Pro may be introduced because of its particularly planar structure, which induces b-turns in the protein's structure. The genes encoding Smurf derivatives and analogs of the invention can be produced by various methods known in the art. The manipulations which result in their production can occur at the gene or protein level. For example, a cloned Smurfl or SmurfZ gene sequence can be modified by any of numerous strategies known in the art (Sambrook et al, 1989, supra). The sequence can be cleaved at appropriate sites with restriction endonuclease(s), followed by further enzymatic modification if desired, isolated, and ligated in vitro. In the production of the gene encoding a derivative or analog of a Smurf, care should be taken to ensure that the modified gene remains within the same translational reading frame as the gene, uninterrupted by translational stop signals, in the gene region where the desired activity is encoded. Additionally, a Smurf-encoding nucleic acid sequence can be mutated in vitro or in vivo, to create and/or destroy translation, initiation, and/or termination sequences, or to create variations in coding regions and/or form new restriction endonuclease sites or destroy preexisting ones, to facilitate further in vitro modification. Preferably, such mutations enhance the functional activity of the mutated Smurf gene product. Any technique for mutagenesis known in the art can be used, including but not limited to, in vitro site-directed mutagenesis (Hutchinson, C, et al, 1978, J. Biol. Chem. 253:6551 ; Zoller and Smith, 1984, DNA 3:479-488; Oliphant et al, 1986, Gene 44:177; Hutchinson et al, 1986, Proc. Natl. Acad. Sci. U.S.A. 83:710), use of TAB" linkers (Pharmacia), etc. PCR techniques are preferred for site directed mutagenesis (see Higuchi, 1989, "Using PCR to Engineer DNA", in PCR Technology: Principles and Applications or DNA Amplification, H. Erlich, ed., Stockton Press, Chapter 6, pp. 61-70).The identified and isolated gene can then be inserted into an appropriate cloning vector. A large number of vector-host systems known in the art may be used. Possible vectors include, but are not limited to, plasmids or modified viruses, but the vector system must be compatible with the host cell used. Examples of vectors include, but are not limited to, E. coli, bacteriophages such as lambda derivatives, or plasmids such as pBR322 derivatives or pUC plasmid derivatives, e.g., pGEX vectors, pMal-c, pFLAG, pGBT9, etc. The insertion into a cloning vector can, for example, be accomplished by ligating the DNA fragment into a cloning vector which has complementary cohesive termini. However, if the complementary restriction sites used to fragment the DNA are not present in the cloning vector, the ends of the DNA molecules may be enzymatically modified. Alternatively, any site desired may be produced by ligating nucleotide sequences (linkers) onto the DNA termini; these ligated linkers may comprise specific chemically synthesized oligonucleotides encoding restriction endonuclease recognition sequences. Recombinant molecules can be introduced into host cells via transformation, transfection, infection, electroporation, etc., so that many copies of the gene sequence are generated. Preferably, the cloned gene is contained on a shuttle vector plasmid, which provides for expansion in a cloning cell, e.g., E. coli, and facile purification for subsequent insertion into an appropriate expression cell line, if such is desired. For example, a shuttle vector, which is a vector that can replicate in more than one type of organism, can be prepared for replication in both E. coli and Saccharomyces cerevisiae by linking sequences from an E. coli plasmid with sequences from the yeast 2μ plasmid.Expression of Smurf PolypeptidesThe nucleotide sequence coding for a Smurf protein, or antigenic fragment, derivative or analog thereof, or a functionally active derivative, including a chimeric protein, thereof, can be inserted into an appropriate expression vector, i.e.. a vector which contains the necessary elements for the transcription and translation of the inserted protein- coding sequence. Such elements are termed herein a "promoter." Thus, the nucleic acid encoding a Smurf protein of the invention is operationally associated with a promoter in an expression vector of the invention. Both cDNA and genomic sequences can be cloned and expressed under control of such regulatory sequences. An expression vector also preferably includes a replication origin.The necessary transcriptional and translational signals can be provided on a recombinant expression vector, or they may be supplied by the native gene encoding a Smurf protein and/or its flanking regions. Potential host-vector systems include but are not limited to mammalian cell systems infected with virus (e.g., vaccinia virus, adenovirus, etc.); insect cell systems infected with virus (e.g., baculovirus); microorganisms such as yeast containing yeast vectors; or bacteria transformed with bacteriophage, DNA, plasmid DNA. or cosmid DNA. The expression elements of vectors vary in their strengths and specificities. Depending on the host- vector system utilized, any one of a number of suitable transcription and translation elements may be used. The host cell containing the recombinant vector comprising the nucleic acid encoding a Smurf protein of the invention is cultured in an appropriate cell culture medium under conditions that provide for expression by the cell. Useful host cells for expression of Smurf include C2C12, 293T, CHO, COS, HEK, Hela, HepG2, NIH3T3, PC12, P19 and other cell lines, and kidney, brain, and bone cells.A recombinant Smurf protein of the invention, or functional fragment, derivative, chimeric construct, or analog thereof may be expressed chromosomally, after integration of the coding sequence by recombination. In this regard, any of a number of amplification systems may be used to achieve high levels of stable gene expression (See Sambrook et al, 1989, supra).Any of the methods previously described for the insertion of DNA fragments into a cloning vector may be used to construct expression vectors containing a gene consisting of appropriate transcriptional/translational control signals and the protein coding sequences. These methods may include in vitro recombinant DNA and synthetic techniques and in vivo recombination (genetic recombination).Expression of a Smurf protein may be controlled by any promoter/enhancer element known in the art, but these regulatory elements must be functional in the host selected for expression. Promoters which may be used to control Smurf gene expression include, but are not limited to, the SV40 early promoter region (Benoist and Chambon, 1981 , Nature 290:304-310), the promoter contained in the 3' long terminal repeat of Rous sarcoma virus (Yamamoto, et al, 1980, Cell 22:787-797), the heφes thymidine kinase promoter (Wagner et al, 1981, Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445), the regulator}' sequences of the metallothionein gene (Brinster et al, 1982, Nature 296:39-42); prokaryotic expression vectors such as the β-lactamase promoter (Villa-Kamaroff, et al. , 1978, Proc. Natl. Acad. Sci. U.S.A. 75:3727-3731), or the tac promoter (DeBoer, et al,1983, Proc. Natl. Acad. Sci. U.S.A. 80:21-25); see also "Useful proteins from recombinant bacteria" in Scientific American, 1980, 242:74-94; promoter elements from yeast or other fungi such as the Gal 4 promoter, the ADC (alcohol dehydrogenase) promoter, PGK (phosphoglycerol kinase) promoter, alkaline phosphatase promoter; and the animal transcriptional control regions, which exhibit tissue specificity and have been utilized in transgenic animals: elastase I gene control region which is active in pancreatic acinar cells (Swift et al, 1984, Cell 38:639-646; Ornitz et al, 1986, Cold Spring Harbor Symp. Quant. Biol. 50:399-409; MacDonald, 1987, Hepatology 7:425-515); insulin gene control region which is active in pancreatic beta cells (Hanahan, 1985, Nature 315:115-122), immunoglobulin gene control region which is active in lymphoid cells (Grosschedl et al. ,1984, Cell 38:647-658; Adames et al, 1985, Nature 318:533-538; Alexander et al, 1987, Mol. Cell. Biol. 7: 1436-1444), mouse mammary tumor virus control region which is active in testicular, breast, lymphoid and mast cells (Leder et al, 1986, Cell 45:485-495), albumin gene control region which is active in liver (Pinkert et al, 1987, Genes and Devel. 1 :268-276). alpha-fetoprotein gene control region which is active in liver (Krumlauf et al,1985, Mol. Cell. Biol. 5:1639-1648; Hammer et al, 1987, Science 235:53-58), alpha 1- antitrypsin gene control region which is active in the liver (Kelsey et al, 1987, Genes and Devel. 1 :161-171), beta-globin gene control region which is active in myeloid cells (Mogram et al, 1985, Nature 315:338-340; Kollias et al, 1986, Cell 46:89-94), myelin basic protein gene control region which is active in oligodendrocyte cells in the brain(Readhead et al, 1987, Cell 48:703-712), myosin light chain-2 gene control region which is active in skeletal muscle (Sani, 1985, Nature 314:283-286), and gonadotropic releasing hormone gene control region which is active in the hypothalamus (Mason et al, 1986, Science 234:1372-1378). A wide variety of host/expression vector combinations may be employed in expressing the DNA sequences of this invention. Useful expression vectors, for example, may consist of segments of chromosomal, non-chromosomal and synthetic DNA sequences. Suitable vectors include derivatives of SV40 and known bacterial plasmids, e.g., E. coli plasmids col El, pCRl, pBR322, pMal-C2, pET, pGEX (Smith et al, 1988, Gene 67:31-40), pMB9 and their derivatives, plasmids such as RP4; phage DNAS, e.g., the numerous derivatives of phage 1, e.g., NM989, and other phage DNA, e.g., M13 and filamentous single stranded phage DNA; yeast plasmids such as the 2m plasmid or derivatives thereof; vectors useful in eukaryotic cells, such as vectors useful in insect or mammalian cells; vectors derived from combinations of plasmids and phage DNAs, such as plasmids that have been modified to employ phage DNA or other expression control sequences, and the like.For example, in a baculovirus expression systems, both non-fusion transfer vectors, such as but not limited to pVL941 (BamHl cloning site; Summers), pVL1393 (BamHl, Smal, Xbal, EcoRl , N tl, Xmalll, Bglll, and Pstl cloning site; Invitrogen), pVL1392 (Bglll, Pstl, Notl, Xmalll, EcoRl, Xbal, Smal, and BamHl cloning site; Summers and Invitrogen), and pBlueifocTH (BamHl, Bglll, Pstl, Ncol, and Hindlll cloning site, with blue/white recombinant screening possible; Invitrogen), and fusion transfer vectors, such as but not limited to pAc700 (BamHl and Kpnl cloning site, in which the BamHl recognition site begins with the initiation codon; Summers), pAc701 and pAc702 (same as pAc700, with different reading frames), pAc360 (BamHl cloning site 36 base pairs downstream of a polyhedrin initiation codon; Invitrogen(l 95)), and pBlueBacHisA, B, C (three different reading frames, with BamHl, Bglll, Pstl, Ncol, and Hindlll cloning site, an Ν-terminal peptide for ProBond purification, and blue/white recombinant screening of plaques; Invitrogen (220)) can be used.Mammalian expression vectors contemplated for use in the invention include vectors with inducible promoters, such as the dihydrofolate reductase (DHFR) promoter, e.g., any expression vector with a DHFR expression vector, or a E>HE/?/methotrexate co-amplification vector, such as pΕD (Pstl, Sail, Sbal, Smal, and EcoRI cloning site, with the vector expressing both the cloned gene and DHFR; see Kaufman, Current Protocols in Molecular Biology, 16.12 (1991)). Alternatively, a glutamine synthetase/methionine sulfoximine co-amplification vector, such as pΕΕ14 (Hindlll, Xbal, Smal, Sbal, EcoRI, and Bell cloning site, in which the vector expresses glutamine synthase and the cloned gene; Celltech). In another embodiment, a vector that directs episomal expression under control of Epstein Barr Virus (EBV) can be used, such as pREP4 (BamHl, Sfil, Xhol, Notl, Nhel, Hindlll, Nhel, Pvull, and Kpnl cloning site, constitutive RSV-LTR promoter, hygromycin selectable marker; Invitrogen), pCEP4 (BamHl, Sfil, Xhol, Notl, Nhel, Hindlll, Nhel, Pvull, and Kpnl cloning site, constitutive hCMV immediate early gene, hygromycin selectable marker; Invitrogen), pMEP4 (Kpnl, Pvul, Nhel, Hindlll, Notl, Xhol, Sfil, BamHl cloning site, inducible methallothionein Ila gene promoter, hygromycin selectable marker: Invitrogen), pREP8 (BamHl, Xhol, Notl, Hindlll, Nhel, and Kpnl cloning site, RSV-LTR promoter, histidinol selectable marker; Invitrogen), pREP9 (Kpnl, Nhel, Hindlll, Notl, Xhol, Sfil. and BamHl cloning site, RSV- LTR promoter, G418 selectable marker; Invitrogen), and pEBVHis (RSV-LTR promoter, hygromycin selectable marker, N-terminal peptide purifiable via ProBond resin and cleaved by enterokinase; Invitrogen). Selectable mammalian expression vectors for use in the invention include pRc/CMV (Hindlll, BstXl, Notl, Sbal, and Apal cloning site, G418 selection; Invitrogen), pRc/RSV (Hindlll, Spel, BstXl, Notl, Xbal cloning site, G418 selection: Invitrogen), and others. Vaccinia virus mammalian expression vectors (see, Kaufman. 1991, supra) for use according to the invention include but are not limited to pSCl 1 (Smal cloning site, TK- and b-gal selection), pMJ601 (Sail, Smal, Afil, Narl, BspMll, BamHl, Apal, Nhel, Sacll, Kpnl, and Hindlll cloning site; TK- and b-gal selection), and pTKgptFlS (EcoRI, Pstl, Sail, Accl, Hindll, Sbal, BamHl, and Hpa cloning site, TK or XPRT selection).Yeast expression systems can also be used according to the invention to express Smurfl . For example, the non-fusion pYΕS2 vector (Xbal, Sphl, Shol, Notl, GstXl, EcoRI, BstXl, BamHl, Sacl, Kpnl, and Hindlll cloning sit; Invitrogen) or the fusion pYΕSHisA, B, C (Xbal, Sphl, Shol, Notl, BstXl, EcoRI, BamHl, Sacl, Kpnl, and Hindlll cloning site, N-terminal peptide purified with ProBond resin and cleaved with enterokinase; Invitrogen), to mention just two, can be employed according to the invention.Once a particular recombinant Smurf DNA molecule is identified and isolated, several methods known in the art may be used to propagate it. Once a suitable host system and growth conditions are established, recombinant expression vectors can be propagated and prepared in quantity. As previously explained, the expression vectors which can be used include, but are not limited to, the following vectors or their derivatives: human or animal viruses such as vaccinia virus or adenovirus; insect viruses such as baculovirus; yeast vectors; bacteriophage vectors (e.g. , lambda), and plasmid and cosmid DNA vectors, to name but a few.In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Different host cells have characteristic and specific mechanisms for the translational and post-translational processing and modification of proteins.Appropriate cell lines or host systems can be chosen to ensure the desired modification and processing of the foreign protein expressed. For example, expression in a bacterial system can be used to produce a nonglycosylated core protein product, while expression in yeast can produce a glycosylated product. Expression in eukaryotic cells can increase the likelihood of "native" folding of a heterologous mammalian protein. Moreover, expression in mammalian cells can provide a tool for reconstituting, or constituting. Smurf activity. Furthermore, it is known in the art that different vector/host expression systems may affect processing reactions (e.g., proteolytic cleavages) to a different extent.Vectors are introduced into the desired host cells by methods known in the art, e.g., transfection, electroporation, microinjection, transduction, cell fusion, DEAE dextran, calcium phosphate precipitation, lipofection (lysosome fusion), use of a gene gun, or a DNA vector transporter (see, e.g., Wu et al, 1992, J. Biol. Chem. 267:963-967; Wu and Wu, 1988, J. Biol. Chem. 263:14621-14624; Hartmut et al, Canadian Patent Application No. 2,012,311, filed March 15, 1990).Analysis of Gene Expression Mediated by Smurf Functional Activity In one embodiment, oligonucleotide array technology can be used, e.g., to evaluate gene expression, after binding of Smurfl to Smadsl or 5, or binding of SmurfZ to Smad7, and to identify gene expression that correlates with, or is distinct from, gene expression in TGFβ or BMP treated cells or cells of injured and/or healing tissue, tissue having cells in a tumorigenic state, and during cellular and developmental processes, such as mitosis, cell differentiation, embryonic pattern formation and development and organogenesis. For example, one could compare gene expression in TGFβ or BMP treated cells in the presence or absence of Smurf expression. GeneChip expression analysis (Affymetrix, Santa Clara, CA) generates data for the assessment of gene expression profiles and other biological assays. Oligonucleotide expression arrays simultaneously and quantitatively interrogate thousands of mRNA transcripts (genes or ESTs), simplifying large genomic studies. Each transcript can be represented on a probe array by multiple probe pairs to differentiate among closely related members of gene families. Each probe cell contains millions of copies of a specific oligonucleotide probe, permitting the accurate and sensitive detection of low-intensity mRNA hybridization patterns. Differential expression data can provide a clear understanding of cellular pathways.After hybridization intensity data is captured, e.g., using a Hewlett-Packard GeneArray™ scanner. Software can be used to automatically calculate intensity values for each probe cell. Probe cell intensities can be used to calculate an average intensity for each gene, which directly correlates with mRNA abundance levels. Expression data can be quickly sorted on any analysis parameter and displayed in a variety of graphical formats for any selected subset of genes. Standard and custom GeneChip expression probe arrays are available today for human, mouse, yeast and other organisms. The GeneChip product line will expand to include expression arrays for the analysis of additional organisms and application areas such as toxicology and pharmacogenomics. Transgenic VectorsSmurf can be introduced into cells to treat a disorder associated with excess BMP or TGFβ activation, such as cancer. Smurf activity can be evaluated by introducing a Smurf vector into a cell and monitoring the cell upon Smurf expression. This can be done in vitro or in vivo, or in vitro followed by transplantation in vivo, also termed ex vivo. Alternatively, as discussed above, Smurf or a Smurf inhibitor (antisense, ribozyme, or intracellular antibody) can be delivered by a vector in modulate Smads, e.g., to prevent Smurf regulation of Smads where BMP or TGFβ activity is desired, such as in bone regeneration, or to study Smurf regulated processes in vivo. Smurf activity can be inhibited by various means, including by delivery of a vector encoding a dominant-negative Smurf derivative (e.g., a Cys710 to Ala mutant) to cells, by antisense nucleic acids (including ribozymes and triple-helix-forming oligonucleotides; these are described in detail supra), and by expression of anti-Smurf intracellular antibodies, e.g., single chain Fv antibodies (see generally Chen, Mol. Med. Today, 3: 160-167, 1997; Spitz et al, Anticancer Res., 16:3415-3422, 1996; Indolfi et al, Nat. Med., 2:634-635, 1996; Kijima et al, Pharmacol. Ther., 68:247-267, 1995).As discussed above, a vector is any means for the transfer of a nucleic acid according to the invention into a host cell. These include viral vectors, such as lentiviruses, retroviruses, heφes viruses, adenoviruses and adeno-associated viruses. Thus, a gene encoding a functional or mutant Smurf protein or polypeptide domain fragment thereof can be introduced in vivo, ex vivo, or in vitro using a viral vector or through direct introduction of DNA. Expression in targeted tissues can be effected by targeting the transgenic vector to specific cells, such as with a viral vector or a receptor ligand, or by using a tissue-specific promoter, or both. Targeted gene delivery is described in International Patent Publication WO 95/28494, published October 1995.Viral vectors commonly used for in vivo or ex vivo targeting and therapy procedures are DNA-based vectors and retroviral vectors. Methods for constructing and using viral vectors are known in the art [see, e.g., Miller and Rosman, BioTechniques 7:980-990 (1992)]. Preferably, the viral vectors are replication defective, that is, they are unable to replicate autonomously in the target cell. In general, the genome of the replication defective viral vectors which are used within the scope of the present invention lack at least one region which is necessary for the replication of the virus in the infected cell. These regions can either be eliminated (in whole or in part), be rendered nonfunctional by any technique known to a person skilled in the art. These techniques include the total removal, substitution (by other sequences, in particular by the inserted nucleic acid), partial deletion or addition of one or more bases to an essential (for replication) region. Such techniques may be performed in vitro (on the isolated DNA) or in situ, using the techniques of genetic manipulation or by treatment with mutagenic agents. Preferably, the replication defective virus retains the sequences of its genome which are necessary for encapsidating the viral particles. DNA viral vectors include an attenuated or defective DNA virus, such as but not limited to heφes simplex virus (HSV), papillomavirus, Epstein Barr virus (EBV), adenovirus, adeno-associated virus (AAV), and the like. Defective viruses, which entirely or almost entirely lack viral genes, are preferred. Defective virus is not infective after introduction into a cell. Use of defective viral vectors allows for administration to cells in a specific, localized area, without concern that the vector can infect other cells. Thus, a specific tissue can be specifically targeted. Examples of particular vectors include, but are not limited to, a defective heφes virus 1 (HSV1) vector [Kaplitt et al, Molec. Cell. Neurosci., 2:320-330 (1991)], defective heφes virus vector lacking a glyco-protein L gene [Patent Publication RD 371005 A], or other defective heφes virus vectors [International Patent Publication No. WO 94/21807, published September 29, 1994; International Patent Publication No. WO 92/05263, published April 2, 1994]; an attenuated adenovirus vector, such as the vector described by Stratford-Perricaudet et al. [J. Clin. Invest., 90:626-630, 1992; see also La Salle et al, Science, 259:988-990, 1993]; and a defective adeno- associated virus vector [Samulski et al, J. Virol, 61 :3096-3101, 1987; Samulski et al, J. Virol., 63:3822-3828,1989; Lebkowski et al, Mol. Cell. Biol., 8:3988-3996, 1988)].Preferably, for in vivo administration, an appropriate immunosuppressive treatment is employed in conjunction with the viral vector, e.g., adenovirus vector, to avoid immuno-deactivation of the viral vector and transfected cells. For example, immunosuppressive cytokines, such as interleukin-12 (IL-12), interferon-g (IFN-g), or anti-CD4 antibody, can be administered to block humoral or cellular immune responses to the viral vectors [see, e.g., Wilson, Nature Medicine (1995)]. In addition, it is advantageous to employ a viral vector that is engineered to express a minimal number of antigens.Adenovirus vectors. Adenoviruses are eukaryotic DNA viruses that can be modified to efficiently deliver a nucleic acid of the invention to a variety of cell types. Various serotypes of adenovirus exist. Of these serotypes, preference is given, within the scope of the present invention, to using type 2 or type 5 human adenoviruses (Ad 2 or Ad 5) or adenoviruses of animal origin (see WO94/26914). Those adenoviruses of animal origin which can be used within the scope of the present invention include adenoviruses of canine, bovine, murine (example: Mavl, Beard et al, Virology 75 (1990) 81), ovine, porcine, avian, and simian (example: SAV) origin. Preferably, the adenovirus of animal origin is a canine adenovirus, more preferably a CAV2 adenovirus (e.g. Manhattan or A26/61 strain (ATCC VR-800), for example).Preferably, the replication defective adenoviral vectors of the invention comprise the ITRs, an encapsidation sequence and the nucleic acid of interest. Still more preferably, at least the El region of the adenoviral vector is non- functional. The deletion in the El region preferably extends from nucleotides 455 to 3329 in the sequence of the Ad5 adenovirus (PvuII-Bglll fragment) or 382 to 3446 (HinfII-Sau3A fragment). Other regions may also be modified, in particular the E3 region (WO95/02697), the E2 region (WO94/28938), the E4 region (WO94/28152, WO94/12649 and WO95/02697), or in any of the late genes L 1 -L5.In a specific embodiment, the adenoviral vector has a deletion in the El region (Ad 1.0). Examples of El-deleted adenoviruses are disclosed in EP 185,573, the contents of which are incoφorated herein by reference. In another embodiment, the adenoviral vector has a deletion in the El and E4 regions (Ad 3.0). Examples of E1/E4- deleted adenoviruses are disclosed in WO95/02697 and WO96/22378, the contents of which are incoφorated herein by reference. In still another preferred embodiment, the adenoviral vector has a deletion in the El region into which the E4 region and the nucleic acid sequence are inserted (see FR94 13355, the contents of which are incoφorated herein by reference). The replication defective recombinant adenoviruses according to the invention can be prepared by any technique known to the person skilled in the art (Levrero et al, Gene 101 :195 1991 ; EP 185 573; Graham, EMBO J. 3:2917, 1984). In particular, they can be prepared by homologous recombination between an adenovirus and a plasmid which carries, inter alia, the DNA sequence of interest. The homologous recombination is effected following cotransfection of the said adenovirus and plasmid into an appropriate cell line. The cell line which is employed should preferably (i) be transformable by the said elements, and (ii) contain the sequences which are able to complement the part of the genome of the replication defective adenovirus, preferably in integrated form in order to avoid the risks of recombination. Examples of cell lines which may be used are the human embryonic kidney cell line 293 (Graham et al, J. Gen. Virol. 36:59 1977) which contains the left-hand portion of the genome of an Ad5 adenovirus (12%) integrated into its genome, and cell lines which are able to complement the El and E4 functions, as described in applications WO94/26914 and WO95/02697. Recombinant adenoviruses are recovered and purified using standard molecular biological techniques, which are well known to one of ordinary skill in the art. Adeno-associated viruses. The adeno-associated viruses (AAV) are DNA viruses of relatively small size which can integrate, in a stable and site-specific manner, into the genome of the cells which they infect. They are able to infect a wide spectrum of cells without inducing any effects on cellular growth, moφhology or differentiation, and they do not appear to be involved in human pathologies. The AAV genome has been cloned, sequenced and characterized. It encompasses approximately 4700 bases and contains an inverted terminal repeat (ITR) region of approximately 145 bases at each end, which serves as an origin of replication for the virus. The remainder of the genome is divided into two essential regions which carry the encapsidation functions: the left-hand part of the genome, which contains the rep gene involved in viral replication and expression of the viral genes; and the right-hand part of the genome, which contains the cap gene encoding the capsid proteins of the virus.The use of vectors derived from the AA Vs for transferring genes in vitro and in vivo has been described (see WO 91/18088; WO 93/09239; US 4,797,368, US 5,139,941, EP 488 528). These publications describe various AAV-derived constructs in which the rep and/or cap genes are deleted and replaced by a gene of interest, and the use of these constructs for transferring the said gene of interest in vitro (into cultured cells) or in vivo, (directly into an organism). The replication defective recombinant AAVs according to the invention can be prepared by cotransfecting a plasmid containing the nucleic acid sequence of interest flanked by two AAV inverted terminal repeat (ITR) regions, and a plasmid carrying the AAV encapsidation genes (rep and cap genes), into a cell line which is infected with a human helper virus (for example an adenovirus). The AAV recombinants which are produced are then purified by standard techniques.Retrovirus vectors. In another embodiment the gene can be introduced in a retroviral vector, e.g., as described in Anderson et al, U.S. Patent No. 5,399,346; Mann et al, 1983, Cell 33:153; Temin et al, U.S. Patent No. 4,650,764; Temin et al, U.S. Patent No. 4,980,289; Markowitz et al, 1988, J. Virol. 62: 1120; Temin et al, U.S. Patent No. 5,124,263; EP 453242, EP178220; Bernstein et al. Genet. Eng. 7 (1985) 235; McCormick, BioTechnology 3 (1985) 689; International Patent Publication No. WO 95/07358, published March 16, 1995, by Dougherty et al ; and Kuo et al, 1993, Blood 82:845. The retroviruses are integrating viruses which infect dividing cells. The retrovirus genome includes two LTRs, an encapsidation sequence and three coding regions (gag, pol and env). In recombinant retroviral vectors, the gag, pol and env genes are generally deleted, in whole or in part, and replaced with a heterologous nucleic acid sequence of interest. These vectors can be constructed from different types of retrovirus, such as, HIV,MoMuLV ("murine Moloney leukaemia virus" MSV ("murine Moloney sarcoma virus"), HaSV ("Harvey sarcoma virus"); SNV ("spleen necrosis virus"); RSV ("Rous sarcoma virus") and Friend virus. Defective retroviral vectors are disclosed in WO95/02697.In general, in order to construct recombinant retroviruses containing a nucleic acid sequence, a plasmid is constructed which contains the LTRs, the encapsidation sequence and the coding sequence. This construct is used to transfect a packaging cell line, which cell line is able to supply in trans the retroviral functions which are deficient in the plasmid. In general, the packaging cell lines are thus able to express the gag, pol and env genes. Such packaging cell lines have been described in the prior art, in particular the cell line PA317 (US4,861,719); the PsiCRIP cell line (WO90/02806) and the GP+envAm-12 cell line (WO89/07150). In addition, the recombinant retroviral vectors can contain modifications within the LTRs for suppressing transcriptional activity as well as extensive encapsidation sequences which may include a part of the gag gene (Bender et al, J. Virol. 61 (1987) 1639). Recombinant retroviral vectors are purified by standard techniques known to those having ordinary skill in the art. Retroviral vectors can be constructed to function as infections particles or to undergo a single round of transfection. In the former case, the virus is modified to retain all of its genes except for those responsible for oncogenic transformation properties, and to express the heterologous gene. Non-infectious viral vectors are prepared to destroy the viral packaging signal, but retain the structural genes required to package the co- introduced virus engineered to contain the heterologous gene and the packaging signals. Thus, the viral particles that are produced are not capable of producing additional virus. Lentivirus vectors. In another embodiment, lentiviral vectors are can be used as agents for the direct delivery and sustained expression of a transgene in several tissue types, including brain, retina, muscle, liver and blood. The vectors can efficiently transduce dividing and nondividing cells in these tissues, and maintain long-term expression of the gene of interest.Lentiviruses contain at least two regulatory genes, tαt and rev, that are essential for replication, and four accessory genes that encode critical virulence factors. [For a review, see Naldini, L., Curr. Opin. Biotechnol., 9:457-63, 1998]. The viral sequences non-essential for transduction are eliminated thereby improving the biosafety of this particular vector. Self- inactivating HIV-1 vectors are known, which have a deletion in the 3' long terminal repeat (LTR) including the TATA box, and significantly improve the biosafety of HIV-derived vectors by reducing the likelihood that replication-competent retroviruses will originate in the vector producer and target cells (Zufferey, et al, J. Virol., 72:9873-80, 1998). In addition, the deletion improves the potential performance of the vector by removing LTR sequences previously associated with transcriptional interference jo and suppression in vivo and by allowing the construction of more-stringent tissue-specific or regulatable vectors.Lentiviral packaging cell lines are available and known generally in the art. They facilitate the production of high-titer lentivirus vectors for gene therapy. An example is a tetracycline-inducible VSV-G pseudotyped lentivirus packaging cell line which can generate virus particles at titers greater than 10(6) IU/ml for at least 3 to 4 days (Kafri, et al, J. Virol., 73: 576-584, 1999). The vector produced by the inducible cell line can be concentrated as needed for efficiently transducing nondividing cells in vitro and in vivo. Non-viral vectors. Alternatively, the vector can be introduced in vivo by lipofection. For the past decade, there has been increasing use of liposomes for encapsulation and transfection of nucleic acids in vitro. Synthetic cationic lipids designed to limit the difficulties and dangers encountered with liposome mediated transfection can be used to prepare liposomes for in vivo transfection of a gene encoding a marker [Feigner, et. al, Proc. Natl. Acad. Sci. U.S.A., 84:7413-7417, 1987; see Mackey, et al, Proc. Natl. Acad. Sci. U.S.A., 85:8027-8031, 1988; Ulmer et α/., Science, 259:1745-1748, 1993]. The use of cationic lipids may promote encapsulation of negatively charged nucleic acids, and also promote fusion with negatively charged cell membranes [Feigner and Ringold, Science, 337:387-388, 1989]. Particularly useful lipid compounds and compositions for transfer of nucleic acids are described in International Patent Publications WO95/18863 and WO96/17823, and in U.S. Patent No. 5,459,127. The use of lipofection to introduce exogenous genes into the specific organs in vivo has certain practical advantages. Molecular targeting of liposomes to specific cells represents one area of benefit. It is clear that directing transfection to particular cell types would be particularly advantageous in a tissue with cellular heterogeneity, such as pancreas, liver, kidney, and the brain. Lipids may be chemically coupled to other molecules for the puφose of targeting [see Mackey, et. al., supra]. Targeted peptides, e.g., hormones or neurotransmitters, and proteins such as antibodies, or non-peptide molecules could be coupled to liposomes chemically.Other molecules are also useful for facilitating transfection of a nucleic acid in vivo, such as a cationic oligopeptide (e.g., International Patent Publication WO95/21931), peptides derived from DNA binding proteins (e.g., International Patent Publication WO96/25508), or a cationic polymer (e.g., International Patent Publication WO95/21931).It is also possible to introduce the vector in vivo as a naked DNA plasmid. Naked DNA vectors for gene therapy can be introduced into the desired host cells by methods known in the art, e.g., transfection, electroporation, micro injection, transduction, cell fusion, DEAE dextran, calcium phosphate precipitation, use of a gene gun, or use of a DNA vector transporter [see, e.g., Wu et al, J. Biol. Chem., 267:963-967, 1992; Wu and Wu, J. Biol. Chem., 263:14621-14624, 1988; Hartmut et al, Canadian Patent Application No. 2,012,311, filed March 15, 1990; Williams et al, Proc. Natl. Acad. Sci. USA, 88:2726-2730, 1991]. Receptor-mediated DNA delivery approaches can also be used [Curiel et al, Hum. Gene Ther., 3:147-154, 1992; Wu and Wu, J. Biol. Chem., 262:4429- 4432, 1987]. US Patent No. 5,580,859 discloses delivery of exogenous DNA sequences, free of transfection facilitating agents, in a mammal.Antibodies against Smurf Proteins According to the invention, a Smurf polypeptide produced recombinantly or by chemical synthesis, and fragments, variants, or other derivatives or analogs thereof, including fusion proteins, may be used as an immunogen to generate antibodies that recognize a Smurf polypeptide. Such antibodies include but are not limited to polyclonal, monoclonal, chimeric, single chain, Fab fragments, and a Fab expression library. Such an antibody is specific for Smurf proteins; it may recognize a mutant form of a Smurf, or wild-type Smurf. These antibodies can be used to alter the BMP pathway by inhibiting a Smurf protein (e.g., anti-Smurf intracellular antibodies) or for diagnostic puφoses.Various procedures known in the art may be used for the production of polyclonal antibodies to a Smurf polypeptide or derivative or analog thereof. For the production of antibody, various host animals can be immunized by injection with a Smurf polypeptide, or a derivative (e.g., fragment or fusion protein) thereof, including but not limited to rabbits, mice, rats, sheep, goats, etc. In one embodiment, a Smurf polypeptide or fragment thereof can be conjugated to an immunogenic carrier, e.g., bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH). Various adjuvants may be used to increase the immunological response, depending on the host species, including but not limited to Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful human adjuvants such as BCG (Bacille Calmette-Gueriή) and Corynebacterium parvum. For preparation of monoclonal antibodies directed toward a Smurf polypeptide, or fragment, analog, or derivative thereof, any technique that provides for the production of antibody molecules by continuous cell lines in culture may be used. These include but are not limited to the hybridoma technique originally developed by Kohler and Milstein (Nature 256:495-497, 1975), as well as the trioma technique, the human B-cell hybridoma technique (Kozbor et al, Immunology Today 4:72, 1983; Cote et al, Proc. Natl. Acad. Sci. U.S.A. 80:2026-2030, 1983), and the EBV-hybridoma technique to produce human monoclonal antibodies (Cole et al. , in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., pp. 77-96, 1985). In an additional embodiment of the invention, monoclonal antibodies can be produced in germ-free animals (International Patent Publication No. WO 89/12690, published 28 December 1989). In fact, according to the invention, techniques developed for the production of "chimeric antibodies" (Morrison et al, J. Bacteriol. 159:870, 1984; Neuberger et α/., Nature 312:604-608, 1984; Takeda et al, Nature 314:452-454, 1985) by splicing the genes from a mouse antibody molecule specific for a Smurf polypeptide together with genes from a human antibody molecule of appropriate biological activity can be used; such antibodies are within the scope of this invention. Such human or humanized chimeric antibodies are preferred for use in therapy of human diseases or disorders (described infra), since the human or humanized antibodies are much less likely than xenogenic antibodies to induce an immune response, in particular an allergic response, themselves.In accordance with the invention, techniques described for the production of single chain antibodies (U.S. Patent Nos. 5,476,786 and 5,132,405 to Huston; U.S. Patent 4,946,778) can be adapted to produce a Smurf polypeptide-specific single chain antibodies. An additional embodiment of the invention utilizes the techniques described for the construction of Fab expression libraries (Huse et al, Science 246:1275-1281, 1989) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity for a Smurf polypeptide, or its derivatives, or analogs. Antibody fragments which contain the idiotype of the antibody molecule can be generated by known techniques. For example, such fragments include but are not limited to: the F(ab')2 fragment which can be produced by pepsin digestion of the antibody molecule; the Fab' fragments which can be generated by reducing the disulfide bridges of the F(ab')2 fragment, and the Fab fragments which can be generated by treating the antibody molecule with papain and a reducing agent. Smurf activity can also be inhibited by expression of anti-Smurf intracellular antibodies, e.g., single chain Fv antibodies, using techniques known in the art (see generally Chen, Mol. Med. Today 3:160-167, 1997; Spitz, et al, Anticancer Res. 16:3415-3422, 1996; Indolfi et al, Nat. Med. 2:634-635, 1996; Kijima et al, Pharmacol. Ther. 68:247-267, 1995). In the production of antibodies, screening for the desired antibody can be accomplished by techniques known in the art, e.g., radioimmunoassay, ELISA (enzyme- linked immunosorbant assay), "sandwich" immunoassays, immunoradiometric assays, gel diffusion precipitin reactions, immunodiffusion assays, in situ immunoassays (using colloidal gold, enzyme or radioisotope labels, for example), western blots, precipitation reactions, agglutination assays (e.g., gel agglutination assays, hemagglutination assays), complement fixation assays, immunofluorescence assays, protein A assays, and immunoelectrophoresis assays, etc. In one embodiment, antibody binding is detected by detecting a label on the primary antibody. In another embodiment, the primary antibody is detected by detecting binding of a secondary antibody or reagent to the primary antibody. In a further embodiment, the secondary antibody is labeled. Many means are known in the art for detecting binding in an immunoassay and are within the scope of the present invention. For example, to select antibodies which recognize a specific epitope of a Smurf polypeptide, one may assay generated hybridomas for a product which binds to a Smurf polypeptide fragment containing such epitope. For selection of an antibody specific to a Smurf polypeptide from a particular species of animal, one can select on the basis of positive binding with a Smurf polypeptide expressed by or isolated from cells of that species of animal.The foregoing antibodies can be used in methods known in the art relating to the localization and activity of a Smurf polypeptide, e.g., for Western blotting, imaging a Smurf polypeptide in situ, measuring levels thereof in appropriate physiological samples, etc., using any of the detection techniques mentioned above or known in the art. Such antibodies can also be used in assays for ligand binding, e.g., as described in US Patent No. 5,679,582.In a specific embodiment, antibodies that agonize or antagonize the activity of a Smurf polypeptide can be generated. Such antibodies can be tested using the assays described infra for identifying ligands.Screening According to the present invention, nucleotide sequences derived from the gene encoding a Smurf, and peptide sequences derived therefrom, are useful targets to identify drugs that are effective in treating disorders mediated by the BMP signaling pathway, as discussed above (see, Schmitt et al, J. Orthopedic Res., 17:269, 1999) can be used to agonize or antagonize a Smurf protein. Drug targets include without limitation (i) isolated nucleic acids derived from the gene encoding a Smurf and (ii) isolated peptides and polypeptides derived from Smurf polypeptides.In particular, identification and isolation of a Smurf protein provides for development of screening assays, particularly for high throughput screening of molecules that up- or down-regulate the activity of a Smurf, e.g., by permitting expression of a Smurf protein in quantities greater than can be isolated from natural sources, or in indicator cells that are specially engineered to indicate the activity of a Smurf protein expressed from a gene after transfection or transformation of the cells. Accordingly, the present invention contemplates methods for identifying specific ligands of a Smurf protein using various screening assays known in the art. Any screening technique known in the art can be used to screen for agonists or antagonists of a Smurf protein. The present invention contemplates screens for small molecule ligands or ligand analogs and mimics, as well as screens for natural ligands that bind to and agonize or antagonize a Smurf protein in vivo. For example, natural products libraries can be screened using assays of the invention for molecules that agonize or antagonize Smurf activity.Knowledge of the primary sequence of Smurfl and SmurfZ, and the similarity of that sequence with proteins of known function, provides one having ordinary skill in the art with useful information to determine inhibitors or antagonists of a Smurf protein. Identification and screening of antagonists is further facilitated by determining structural features of the protein, e.g., using X-ray crystallography, neutron diffraction, nuclear magnetic resonance spectrometry, and other techniques for structure determination. These techniques provide for the rational design or identification of agonists and antagonists. Another approach uses recombinant bacteriophage to produce large libraries. Using the "phage method" (Scott and Smith, Science 249:386-390, 1990; Cwirla, et al, Proc. Natl. Acad. Sci., 87:6378-6382, 1990; Devlin et al, Science, 49:404- 406, 1990), very large libraries can be constructed (106-108 chemical entities). A second approach uses primarily chemical methods, of which the Geysen method (Geysen et al, Molecular Immunology 23:709-715, 1986; Geysen et al. J. Immunologic Method 102:259- 274, 1987; and the method of Fodor et al. (Science 251 :767-773, 1991) are examples. Furka et al. (14th International Congress of Biochemistry, Volume #5, Abstract FR:013, 1988; Furka, Int. J. Peptide Protein Res. 37:487-493, 1991), Houghton (U.S. Patent No. 4,631,211, issued December 1986) and Rutter et al. (U.S. Patent No. 5,010,175, issued April 23, 1991) describe methods to produce a mixture of peptides that can be tested as agonists or antagonists.In another aspect, synthetic libraries (Needels et al. , Proc. Natl. Acad. Sci. USA 90: 10700-4, 1993; Ohlmeyer et al, Proc. Natl. Acad. Sci. USA 90: 10922-10926, 1993; Lam et al, International Patent Publication No. WO 92/00252; Kocis et al, International Patent Publication No. WO 9428028) and the like can be used to screen for Smurf ligands according to the present invention.Test compounds are screened from large libraries of synthetic or natural compounds. Numerous means are currently used for random and directed synthesis of saccharide, peptide, and nucleic acid based compounds. Synthetic compound libraries are commercially available from Maybridge Chemical Co. (Trevillet, Cornwall, UK),Comgenex (Princeton, NJ). Brandon Associates (Merrimack, NH), and Microsource (New Milford, CT). A rare chemical library is available from Aldrich (Milwaukee, WI). Alternatively, libraries of natural compounds in the form of bacterial, fungal, plant and animal extracts are available from e.g. Pan Laboratories (Bothell, WA) or MycoSearch (NC), or are readily producible. Additionally, natural and synthetically produced libraries and compounds are readily modified through conventional chemical, physical, and biochemical means (Blondelle et al, Tib Tech, 14:60, 1996).In vitro screening methods In one series of embodiments, an isolated nucleic acid comprising a Smurf gene is tested in vitro for its ability to bind test compounds in a sequence-specific manner. The methods comprise:(i) providing a nucleic acid containing a particular sequence corresponding to all or portions of a Smurf protein;(ii) contacting the nucleic acid with a multiplicity of test compounds under conditions appropriate for binding; and (iii) identifying those compounds that bind selectively to the nucleic acid sequence.Selective binding as used herein refers to any measurable difference in any parameter of binding, such as, e.g., binding affinity, binding capacity, etc.In one series of embodiments, an isolated peptide or polypeptide, or fragments thereof, comprising a Smurf protein is tested in vitro for its ability to bind test compounds in a sequence-specific manner. The screening methods involve:(i) providing a peptide or polypeptide, or fragment thereof, corresponding to Smurf protein or a fragment thereof;(ii) contacting the peptides with a multiplicity of test compounds under conditions appropriate for binding; and(iii) identifying those compounds that bind selectively to the peptides. In preferred embodiments, high-throughput screening protocols are used to survey a large number of test compounds for their ability to bind the genes or peptides disclosed above in a sequence-specific manner. In vivo screening methodsIntact cells or whole animals expressing variants of a gene encoding a Smurf protein can be used in screening methods to identify candidate drugs. The following methods can be applied to normal or wild-type SmurfIn one series of embodiments, a permanent cell line is established from an individual exhibiting expression of a variant Smurf gene. Alternatively, cells (including without limitation mammalian, mammalian, insect, yeast, and bacterial cells) are programmed to express a gene comprising one or more variant Smurf sequences by introduction of suitable vector. Identification of candidate compounds can be achieved using any suitable assay, including without limitation (i) assays that measure selective binding of test compounds to particular variant of a Smurf; (ii) assays that measure the ability of a test compound to modify (i.e. , inhibit or enhance) a measurable activity or function of the Smurf; and (iii) assays that measure the ability of a compound to modify (i.e., inhibit or enhance) the transcriptional activity of sequences derived from the promoter (i.e., regulatory) regions of a Smurf gene.In another series of embodiments, transgenic animals are created in which (i) a human Smurf, having one or more mutations is stably inserted into the genome of the transgenic animal; and/or (ii) endogenous Smurf genes are inactivated and replaced with a variant human Smurf gene. See, e.g., Coffman, Semin. Nephrol. 17:404, 1997; Esther et al, Lab. Invest. 74:953, 1996; Murakami et al, Blood Press. Suppl. 2:36, 1996. A preferred method for creating such a transgenic animal is so called "knock-in" technology, where a human gene can be inserted to replace an endogenous gene under expression control of the endogenous genes' regulatory elements (see, Rodriguez et al, Cell, 97:199, 1999). Such animals can be treated with candidate compounds and monitored for any alteration in the BMP and TGFβ/activin pathways.Furthermore, populations that are not amenable to an established treatment for tissue and bone degeneration (e.g. , osteoporosis) or enhancing regeneration can be selected for testing of alternative treatments. Moreover, treatments that are not as effective in the general population, but that are highly effective in the selected population, may be identified that otherwise would be overlooked. This is an especially powerful advantage of the present invention, since it eliminates some of the randomness associated with clinical trials.High-Throushput Screen Agents according to the invention may be identified by screening in high- throughput assays, including without limitation cell-based or cell-free assays. It will be appreciated by those skilled in the art that different types of assays can be used to detect different types of agents. Several methods of automated assays have been developed in recent years so as to permit screening of tens of thousands of compounds in a short period of time. Such high-throughput screening methods are particularly preferred. The use of high-throughput screening assays to test for agents is greatly facilitated by the availability of large amounts of purified polypeptides, as provided by the invention. Reporter GenesSmad proteins have been identified as mediators of intracellular signal transduction by members of the transforming growth factor-beta (TGF-beta) superfamily, which affect cell proliferation, differentiation, as well as pattern formation during early vertebrate development. Following receptor activation, Smads are assembled into heteromeric complexes consisting of a pathway-restricted Smad and the common Smad4 that are subsequently translocated into the nucleus where they are thought to play an important role in gene transcription. Smad Binding Elements (SBEs) containing the sequence CAGACA in the promoter of the JunB gene, is an immediate early gene that is potently induced by TGF-beta, activin, and bone moφhogenetic protein (BMP) 2 (Jonk et al, J. Biol. Chem., 273:21145, 1998). Two JunB SBEs are arranged as an inverted repeat, which is transactivated in response to Smad3 and Smad4 co-overexpression and shows inducible binding of a Smad3- and Smad4-containing complex in nuclear extracts from TGF-beta-treated cells. Smad proteins bind directly to the SBE. Multimerization of the SBE creates a powerful TGF-beta-inducible enhancer that is also responsive to activin and BMPs (see, e.g., Kim et al, Nature, 388:304, 1997).Reporter gene expression can be tied to expression or activation of any component of the BMP signaling pathway. Any reporter gene known in the art can be used. Thus, nucleotide sequences having the SBE sequence can be isolated using techniques known in the art and operably linked to a reporter gene to determine whether a Smurf, derivatives, and variants thereof, alter the activity of such promoter. In addition, other known genes in the BMP signaling pathway, such as Tlx2 or Smad7, which have promoters that are regulated by Smadl and 5 can be operably linked to a reporter gene. In these assays, candidate compounds can be tested for their ability to alter BMP of TGFβ signaling in cells that express Smadl, Smad5, Smad7, Smurfl or SmurfZ, or derivatives or variants thereof.Various reporter gene assays are known and can be used to evaluate the effects of a Smurf on a BMP or TGFβ/activin signaling pathway. Reporter genes include luciferase, β-galactosidase (β-gal or lac-Z), chloramphenicol acetyltransferase (CAT), horseradish peroxidase, and alkaline phosphatase. In addition, expression of almost any protein can be detected using a specific antibody. For example, a green fluorescent protein (GFP) expression assay permits evaluation of Smad-induced SBE activity. GFP has been modified to produce proteins that remain functional but have different fluorescent properties. Several U.S. patents teach use of GFP to visualize signaling pathways linked to the reporter gene by an inducible promoter. For example, see U.S. Patent No. 5,625,048 (modified GFP resulting in amino-acid changes provides visibly distinct colors and increased intensities of emission), WO96/23898 (construct encoding a modified GFP which also contains an enzyme recognition site), WO97/11094 (fluorescent proteins with increased intensity), WO97/266333 (humanized GFP protein optimized to provide higher levels of expression in mammalian cells), WO97/42320 (modified GFP having increased intensity of fluorescence, WO98/06737 (modified GFP easily distinguished from green and blue fluorescent proteins), WO98/21355 (GFP mutants excitable using blue and white light).Screening Kits The components required to practice the screening methods described above can be prepared in kit form, for the convenience of the user. Such kits are preferably adapted for use in an automated screening apparatus.Identification of Smurfl Binding PartnersIn still another embodiment, the present invention provides for identification of Smurf binding partners, in addition to Smadl, Smad7 and Smad5, which can then be analyzed for mutations that lead to diseases mediated by the BMP or TGFβ/activin pathways. One method for identifying such binding partners is a yeast two hybrid assay system, preferably using a hematopoietic stem cell library with yeast that are transformed with a recombinant Smurf. Alternatively, a Smurf protein can be used to affinity purify proteins from cell preparations, e.g. , using cells that endogenously produce Smurfs. Partially purified preparations can be probed with a labeled Smurf protein to identify specific binding partners, e.g. , in a Western-type or other antibody-assay type of system (see the description above of antibodies for examples of such assays; naturally, any protein can be labelled as an antibody and its binding to a binding partner evaluated).EXAMPLESThe present invention may be better understood by reference to the following examples, which are not intended to limit the invention.EXAMPLE 1Smurfl targets the BMP pathway and affects embryonic pattern formation. The TGFβ superfamily regulates diverse biological processes including cell growth, differentiation and pattern formation. Any misregulation of TGFβ signals may cause disease. Signals from activated TGFβ receptors are directly transduced from receptor to nucleus by Smad proteins. Currently, a few links have been established between ubiquitin-mediated degradation and the modulation of moφhogenetic signaling during development. This Example describes a new E3 ubiquitin ligase, Smurfl , that selectively interacts with BMP pathway-specific Smads to trigger their ubiquitination, degradation and loss of activity. In amphibian embryos, Smurfl specifically blocked BMP signals and affected pattern formation. Thus, targeted ubiquitination of Smads may function to control both embryonic development and a wide variety of cellular responses to TGFβ signals.Methods Yeast two-hybrid screen. A Xenopus Smadl cDNA (36) was cloned into the pGBT9 vector and used to screen a Xenopus oocyte cDNA library (Clonetech) by the yeast two-hybrid method (46) using Xenopus Smadl as the bait protein. A partial cDNA was isolated and used to screen a Xenopus Stage9 (blastula) cDNA library to obtain a full length Smurfl cDNA [SEQ ID NO: 1]. A human Smurfl cDNA encoding all but the first 8 amino acids was identified in the EST database (AA292123), and was used to construct human Smurfl . The first 8 amino acids were reconstituted using the corresponding Xenopus Smurfl cDNA sequence. hSmurf cDNA was FLAG tagged at its N-terminus. The ubiquitin ligase mutant version of hSmurfl was generated by replacing cysteine 710 with alanine using a PCR-based approach and the mutation was confirmed by sequencing. Co-immunoprecipitation. For immunoprecipitation assays Xenopus Smadl (36), mouse Smad4, and human Smad2 (47), were FLAG-tagged at their C-termini and translated in vitro (rabbit reticulocyte extracts; Promega) in the presence of 35S-Met. The FLAG-tagged Smads were bound to anti-FLAG antibody-conjugated beads (Kodak), washed in co-IP buffer (lOmM Tris, pH 7.5, 90 mM NaCl, 1 mM EDTA, 1% Triton-X 100, 10% glycerol, 1 mM phenylmethylsulfonylflouride) then incubated with 35S-Met-labelled Smurfl in the same buffer. After washing in co-IP buffer and elution in gel loading buffer, proteins were separated by SDS-PAGE and visualized by autoradiography.Embryo methods and RT-PCR. Embryo production, preparation and injection of synthetic mRNA into embryos, animal cap and ventral marginal zone assays, embryonic RNA isolation, embryo fixation and whole mount in situ hybridization, and developmental RT-PCR were performed as described (36, 48). Primers for Smurfl RT-PCR were 5'-GTCCTGTGACTGGAACCC-3' (sense) [SEQ ID NO: 5] and 5'-.GAGGACTGCTAGACAAT-3' (antisense) [SEQ ID NO: 6], whose 5' ends are respectively located at positions 482 and 726 in the Smurfl cDNA. mSmurfl. Northern blots were performed of mSmurfl expression in embryonic and adult mouse tissues. Equal amounts of Poly A+ mRNA from embryonic tissue 7, 11 and 15 days post coitum and tissue samples from testes, kidney, skeletal muscle, lung, spleen, brain and heart were analyzed. Cytoskeletal actin expression was assayed on the same blot and verified that mRNA loading was equal in all lanes. Whole-mount in situ hybridization on mouse embryos were also performed.Immunoprecipitations and Immunoblotting. COS-1 and 293T cells were transiently transfected using lipofectAMINE (GibroBRL) and calcium phosphate precipitation methods, respectively (49). Immunoprecipitations and immunoblotting were performed as described previously (50) using anti-Flag M2 monoclonal antibody (Sigma), anti-Smadl/5 polyclonal antibody (50) or anti-WW2 Nedd4 (51) polyclonal antibody. Detection was achieved using the appropriate HRP conjugated goat anti-mouse or goat anti-rabbit secondary antibodies and enhanced chemiluminescence (Amersham).Pulse Chase Analysis. COS-1 cells were transfected as indicated above. Two days post-transfection the cells were labelled for 10 min. at 37° C with 50 μCi [35S]-methionine/ml in methionine-free DMEM (Pulse). Cell layers were then washed two times and incubated in DMEM+10% FCS for the indicated time periods (Chase). At each time point of the chase, cell lysates prepared in TNTE lysis buffer (50 mM Tris/HCl, pH 7.4, 150 mM NaCl, 0.5% Triton X-100 and ImM EDTA) containing protease and phosphatase inhibitors were subjected to immunoprecipitations using an anti-Smadl polyclonal antibody. Immune complexes were resolved by SDS-PAGE and visualized by autoradiography. A Phosphorimager (Molecular Dynamics) was used to quantitate the amount of metabolically labelled Smadl present at each time point.Ubiquitination Assay. 293 T cells were transfected with HA-tagged ubiquitin, untagged-Smadl, and either Flag-hSmurfl or Flag-hSmurfl (C710A) as indicated above. Two days post-transfection, cells were lysed and subjected to a-Smadl immunoprecipitation. The immunoprecipitates were then washed sequentially two times each in TNTE + 0.1 % triton, SDS-RIPA (TNTE lysis buffer, 0.1 % sodium dodecyl sulfate and 1% deoxycholate), and 500 mM LiCl, 50 mM Tris/HCl, pH 7.4 and 0.1% trition. The presence of HA-ubiquitinated Smadl in the immune complexes was visualized by SDS-PAGE followed by immunoblotting with the monoclonal anti-HA 12CA5. Protein levels of untagged Smadl, Flag-hSmurfl and Flag-hSmurfl (C710A) were analyzed by immunoblotting aliquots of total cell lysates with the appropriate antibodies.Results Isolation of an E3 ubiquitin ligase that interacts with the BMP signal transducer Smadl. To isolate factors that interact with and potentially regulate Smadl we performed a yeast two hybrid screen using Xenopus Smadl as the bait protein. Several overlapping cDNAs were isolated that encoded a protein with significant homology to the Hect subclass of E3 ubiquitin ligases (22). This novel gene and a closely related human homolog, Smurfl and hSmurfl, respectively (for Smad ubiquitination regulatory factor- 1), are proteins of 731 amino acids in length, share 91% sequence identity and contain a Hect ubiquitin-ligase domain in the C-terminal portion of the molecule. This domain is characteristic of a specific class of E3 ubiquitin ligases (22) that include in mammals, E6-AP and Nedd4 and in yeast, RSP5p and Publ (23-26).Identification of human Smurfl genomic location. The human Smurfl genomic clone is located in PAC clone DJ0808A01, from chromosomal region 7q21.1-q31.1, located from nucleotides 2669..53763. A computer prediction of intron/exon splicing products generated a single conceptual mRNA with open reading frame directly corresponding to the actual cloned human cDNA.E3 ubiquitin ligases function together with El and E2 enzymes to conjugate ubiquitin to specific protein substrates, which targets the protein for subsequent degradation by the proteasome (12). El enzymes recruit and activate ubiquitin, which is then transferred to E2, a ubiquitin conjugating enzyme. The E2 in turn attaches ubiquitin either directly to the protein to be modified, or transfers ubiquitin to the E3 ubiquitin ligase, which confers substrate selectivity to the ubiquitination complex. By functioning to recruit specific target proteins to the conjugation machinery, E3 activity provides selectivity to the ubiquitination process. E3 activity can be provided by structurally and functionally diverse proteins. For instance, E3s may exist as multimeric protein complexes that facilitate substrate recognition, but may or may not possess direct ligase activity. Examples of this include the N-end rule E3s, and the Skpl/Cullin F-box (SCF) complexes (27-29). In the case of the Hect family of E3 ubiquitin ligases, of which Smurfl is a member, a single protein provides both substrate specificity and catalytic activity (22, 30). Smurfl also displays several other structural features characteristic of RSP5, Nedd4 and Publ ubiquitin ligases. This includes an amino-terminal phospholipid and calcium binding C2 domain, as well as two WW domains, which facilitate protein-protein interactions by binding to PPXY motifs on partner proteins (31-33). Overall, Smurfl is most closely related to Publ (Fig. 1), a ubiquitin ligase fromSacromyces pombe that regulates mitosis by targeting cdc25 for proteosomal degradation (23).Expression of mSmurfl in embryonic and adult tissue. Northern blots of mSmurfl showed that a transcript of about 6 kb was the predominant form of Smurfl expressed in embryos (Fig. 2A). In tissues, major transcripts of 3.0 and 6.0 kb were observed (Fig. 2B). Cytoskeletal actin expression was assayed on the same blot and verified that mRNA loading was equal in all lanes. In the mouse, mSmurfl was present in very early development, from at least embryonic day 7 onward. In adults, mSmurfl was expressed in most tissues assayed, but there were differences in the abundance of the transcripts. We did not observe detectable mSmurfl transcripts in the skeletal muscle, and the kidney and spleen expressed low levels of the large transcript. The small transcript was very abundant in the testes, and it was very weakly or not at all expressed in the kidney, lung, spleen, brain and heart. Both blots were prepared by loading equal amounts of poly A+ RNA in each lane, but to account for any variation in RNA loading and transfer, we probed the blot with a tubulin probe, and found no substantial differences among lanes. Thus, the variation seen in the blots reflected the relative expression of mSmurfl in embryos and tissues.Whole-mount in situ hybridization on mouse embryos revealed Smurfl expression in the nervous system, eye, branchial arches, axial mesoderm (notochord), somites, and limb buds from days p.c. 10 - 14.Localization of Xenopus Smurfl to the egg animal pole and embryonic ectoderm. In Xenopus embryonic development, Smurfl mRNA was found expressed from egg through swimming tadpole stages, with maximum levels observed early in development at egg, blastula, and gastrula stages. Smurfl levels declined shaφly near the end of gastrulation, yet zygotic expression persisted at a reduced level into late swimming tadpole stages (Fig. 3A). Whole mount in situ hybridization (Fig. 3B) revealed that maternal Smurfl transcripts were localized to the animal pole half of eggs and cleaving blastulae, which was confirmed by a northern blot analysis on RNA isolated from animal and vegetal halves of middle blastula stage embryos (not shown). At gastrulation, Smurfl expression became more widespread in the embryo, coincidently with the decline in its transcript levels observed by RT-PCR. During neural plate closure, however, Smurfl expression was localized to the developing nervous system, and by tadpole stages it was highly expressed in the central nervous system, eye, pharyngeal pouches and somites. The embryonic expression pattern of Smurfl partially overlaps with the expression of Smadl and BMP-4 in the ectoderm at blastula and gastrula stages and the nervous system, eye, somites and pharyngeal pouches at tadpole stages (34-36).Expression of hSmurfl leads to selective reduction in the steady-state protein level of Smadl and Smad5 in mammalian cells. Smurfl contains a putative E3 ligase or Hect domain, and therefore was a candidate protein for interacting with Smadl. To investigate whether Smurfl regulates steady-state levels of Smadl protein, two mammalian cell lines, 293 T and COS-1, were transfected with Smadl alone or with increasing amounts of Flag-hSmurfl . The steady-state levels of Smadl protein were then evaluated by immunoblots of whole cell lysates. Smadl was readily detectable in the absence of hSmurfl (Fig. 4A). However, expression of hSmurfl, even at low levels not readily detectable by Western blotting, produced a significant, dose-dependent decrease in Smadl protein levels. At the highest levels of hSmurfl expression there was no detectable Smadl protein. Furthermore, co-expression of a constitutively activated form of the BMP type I receptor ALK6, which phosphorylates Smadl (37, 38), did not alter hSmurfl -dependent decreases in Smadl levels (Fig. 4B). Thus, expression of hSmurfl causes dose-dependent decreases in steady-state levels of Smadl . This type of action can occur independently of activation of Smadl by the type I BMP receptor.To investigate whether Smurfl activity is exclusive to Smadl, we investigated its effects on Smad2, a receptor-regulated Smad that functions in TGFβ and activin signaling pathways. Unlike Smadl, which was sensitive to the lowest doses of hSmurfl , there was only a slight effect on steady-state levels of Smad2 protein, which occurred only at the highest levels of Flag-hSmurfl expression (Fig. 4C). Other Smads were tested: Flag-hSmurfl had little or no effect on Smad3 or Smad4 protein levels, but it elicited a strong decrease in Smad5 protein, which is closely related to Smadl (Fig. 4D). Together, these data demonstrate that hSmurfl preferentially regulates the steady-state levels of Smadl and Smad5, two receptor-regulated Smads that function in BMP signaling. O 00/77168 . P T/hSmurfl regulates Smadl degradation and ubiquitination. The inclusion of a putative E3 ligase or Hect domain supported the prediction that Smurfl functions as an E3 ubiquitin ligase. To investigate whether Smurfl regulates Smad degradation, studies focused on Smadl (91% homology with Smad5). Analysis of Smadl turnover by pulse-chase experiments revealed that in the absence of hSmurfl, Smadl had a half life of approximately 6 hours. However, in the presence of hSmurfl, Smadl turnover was significantly enhanced (half life of less than 2 hours) (Fig. 5A). Thus, hSmurfl increases the rate of Smadl turnover.To determine the mechanism of hSmurfl -mediated turnover of Smadl, Smadl ubiquitination in intact cells was assessed. To facilitate detection of ubiquitin, 293T cells were transfected with HA-tagged ubiquitin together with Smadl, in the presence or absence of Flag-hSmurfl . In the absence of hSmurfl, Smadl displayed little or no detectable ubiquitination. However, upon co-transfection with hSmurfl we observed the appearance of a ladder of ubiquitin-conjugated Smadl (Fig. 5B). To confirm that ubiquitination of Smadl required the catalytic activity of the Hect domain in hSmurfl, a point mutant in hSmurfl (hSmurfl (C710A)) was constructed. This residue is critical for the catalytic activity of the Hect domain and the mutation is thought to target the cysteine residue that forms a thiolester bond with ubiquitin (22). In contrast to wildtype hSmurfl, expression of hSmurfl (C710A) did not yield ubiquitinated Smadl. Moreover, hSmurfl (C710A) did not affect Smadl steady-state protein levels compared to wildtype hSmurfl, despite efficient expression of the mutant protein (Fig. 5C). Together, these data suggest that hSmurfl alters Smadl steady-state levels by inducing ubiquitin-mediated degradation of Smadl via its Hect domain.Smurfl interacts selectively in vivo with Smadl and Smad5. The interaction of Smurfl with Smad proteins was investigated to assess the basis for the selective targeting of Smadl and 5 for degradation by Smurfl . The yeast 2-hybrid assay was first used to test the ability of Xenopus Smurfl to interact with Smadl, Smad4 or the non-specific control protein nuclear lamin. Yeast co-transfected with Smurfl and Smadl exhibited significant β-galactosidase activity; yeast co-transfected with Smurfl and either Smad4 or lamin did not demonstrate β-galactosidase activity (Fig. 6A, left). Smurfl selectively bound to Smadl, but not to Smad2 or Smad4 (Fig. 6 A, right) as demonstrated by the capacity of 35S-labelled Smurfl to bind and immunoprecipitate Smads in vitro. To test the capacity of 35S-labelled Smurfl to bind and immunoprecipitate Smads in vitro the specificity of Smurfl -Smad interactions in intact cells, the association of hSmurfl with various Smads in 293T cells was investigated. Wild type hSmurfl did not detect interactions with Smadl. However, it is possible that in intact cells Smurfl -Smadl interactions are transient in nature, since demonstrating association between ubiquitin ligases and their substrates has proven difficult in other systems. By examining Smadl interaction with the ubiquitin-ligase mutant Flag-hSmurfl (C710A), association of the proteins could be detected (Figs. 6B, C). Furthermore, this interaction was unaffected by coexpression of the constitutively active BMP -type I receptor, ALK2 (data not shown), and is consistent with the notion that hSmurfl regulates Smadl turnover independent of BMP signaling. hSmurfl (C710A) also bound efficiently to Smad5, but analysis of association with Smad2 revealed little, if any, interaction (Figs. 6A and B).The linker regions of R-Smads contain a PPXY sequence, which is a conserved motif recognized by WW domains such as those found in Smurfl . Unlike wild type Smadl, a mutant of Smadl, in which the PY motif was deleted, associated only weakly with Smurfl and was resistant to Smurf 1 -mediated degradation (data not shown). These results show that hSmurfl associates specifically with Smadl and Smad5, which interaction is mediated by binding between the PY motif in Smadl and the WW domains of Smurfl . The specificity of hSmurfl as a ubiquitin ligase in targeting the Smads, was further tested by comparing it to Nedd4, a structurally related ubiquitin ligase. While Smadl co-precipitated efficiently with hSmurfl (C710A), it did not interact with the corresponding Nedd4 mutant. Consistent with this, overexpression of Nedd4 did not affect the steady-state level of Smadl protein (Fig. 6C, lower panel). Together, these data indicate that both human and Xenopus Smurfl proteins associate selectively withBMP -regulated Smadl and Smad5, and that this interaction is specific to Smurfl rather than a general feature of the Hect class of ubiquitin ligases.Smurfl antagonizes endogenous BMP signals in Xenopus embryos to dorsalize ventral mesoderm and neuralize ectoderm. In the Xenopus blastula, Smurfl expression is localized to the animal pole ectoderm and partly overlaps the marginal zone, i.e., a belt of cells located at the equator of the blastula which forms the mesoderm. This pattern of expression, considered together with the ability of Smurfl to interact with, ubiquitinate, and degrade BMP pathway-specific Smads, suggests that Smurfl may function in ectodermal and mesodermal patterning by antagonizing BMP signals through Smadl or Smad5.Presently, naturally-occurring inhibitors of TGFβ activities have been described at the ligand level. Those factors, which include chordin, follistatin and noggin, act outside of cells by binding particular ligands, including BMPs and activin. During Xenopus mesoderm induction and patterning, BMP signals in the ventral part of the marginal zone specify development of tissues, such as blood and mesenchyme, that are characteristic of the ventral region of the tadpole. However, if BMP signaling is blocked by ligand antagonists such as chordin, follistatin, or noggin (which are secreted by the dorsal Spemann organizer), or by artificial inhibitors such as dominant negative BMP ligands or receptors, the prospective ventral mesoderm will differentiate into dorsal tissues such as muscle and notochord (a process referred to as "dorsalization") (5, 39).Smurfl has the potential to regulate BMP signals in the marginal zone, so ectopic Smurfl expression was tested for interference with ventral tissue patterning. Smurfl mRNA was microinjected into 4 cell blastula stage embryos in the ventral marginal zone (VMZ) to trigger the formation of ectopic secondary axial structures in the ventral region of 52% of tadpoles (n = 25). These secondary axial structures induced by Smurfl were characteristic of dorsalization caused by BMP inhibition. Their formation was rescued by co-expression of Smadl together with Smurfl , demonstrating that the effect of Smurfl was limited to interference with the BMP/Smadl pathway (Fig. 7A).Furthermore, overexpression of Smurfl in cells of the dorsal marginal zone (DMZ), which forms head and dorsal axial structures, had no effect (data not shown). This latter observation was consistent with previous findings of the invention that Smurfl does not target Smad2 in cultured cells. The dorsalizing effects of Smurfl using VMZ explants was further characterized. In this case, Smurfl expression caused reduced blood differentiation and concomitant muscle differentiation, consistent with the dorsalizing effect of Smurfl (Fig. 7A, right panel). As in the axis formation assay, co-expression of Smadl with Smurfl reversed these dorsalizing effects and demonstrated BMP pathway specificity. In the ectodermal germ layer, endogenous BMP expression specifies epidermis, but when BMP signals are reduced or eliminated the ectoderm differentiates into cement gland or neural tissue, respectively (40, 41). The localization of Smurfl mRNA in the ectoderm of the Xenopus blastula and early gastrula suggested that Smurfl may regulate ectodermal patterning by modulating BMP signaling. Therefore, Smurfl was tested for its presence in ectodermal tissue. It was found that overexpression of Smurfl in animal caps triggers neural and cement gland differentiation, which is characteristic of a reduction in BMP signaling. These effects were reversed by co-expression of Smadl in the animal cap (Fig. 7B). Together these results demonstrate that Smurfl can block BMP signals in the ectoderm and mesoderm, suggesting that Smurfl decreases BMP signaling in these tissues to affect embryonic patterning.Smurfl inhibits Smadl activity but potentiates Smadl activity in embryonic cells. In cultured cells, Smurfl triggers ubiquitination and degradation of BMP pathway-specific Smads, and in Xenopus embryos Smurfl antagonizes endogenous BMP signals. In Xenopus animal caps overexpression of Smads mimics the effects of TGFβ factors that signal through specific R-Smads. Thus, Smadl induces exclusively ventral/posterior mesoderm, like BMP ligands, while Smad2 induces dorsal (Spemann organizer) mesoderm, like activin, Vgl and nodal. We therefore tested whether Smurfl can directly antagonize the mesoderm induction activities of Smadl or Smad2 by overexpressing each Smad together with various doses of Smurfl in animal caps. We found that expression of Smadl alone (1 ng mRNA) induced ventral mesoderm, as demonstrated by expression of the ventral/posterior mesodermal markers Xhox3 and Xcadl . However, co-expression of Smurfl and Smadl blocked induction of these markers at all Smurfl doses tested (Fig. 8 A), demonstrating that Smurfl can antagonize Smadl activity.To determine whether Smurfl can interfere with Smad2, a dose of Smad2 (50 pg mRNA) was used in an amount sufficient to induce myoD (a vertebrate muscle marker of dorsal/lateral mesoderm), but insufficient to induce goosecoid (a frog marker for dorsal mesoderm of the Spemann Organizer) (Figs. 7B, C). When Smurfl was co-expressed with Smad2 there was no inhibition of myoD induction at any of the Smurfl doses tested (Fig. 8B). This was consistent with other findings that Smurfl does not target Smad2. Interestingly, as the dose of Smurfl was increased in the presence of this limiting amount (50 pg) of Smad2, induction of goosecoid gene expression was observed. This induction was dependent on Smad2 expression, since Smurfl alone did not induce goosecoid. The response of cap cells to increasing amounts of Smurfl in the presence of limiting Smad2 had similar effects to increasing the dose of Smad2 alone (Fig. 8C). Thus, a combination of 50 pg Smad2 and 100 pg Smurfl induced goosecoid expression to a level equivalent to a 5-fold higher (250 pg) dose of Smad2 alone (Fig. 8C). Thus, rather than inhibit Smad2 activity, Smurfl appears to enhance the sensitivity of the animal cap to Smad2. These experiments demonstrate that in addition to blocking the response of animal pole cells to the BMP pathway, Smurfl enhances these cells' response to the activin pathway. Although the invention is not dependent on any particular mechanism, the inventors propose that the Smurfl -mediated shift in responsiveness of animal pole cell results from lowered endogenous BMP signaling caused by targeted ubiquitination of the Smurfl substrates, Smads 1 and 5. Therefore, Smurfl functions during development to alter the competence of cells to respond to multiple TGFβ ligands by selective inactivation of a particular Smad pathway.Discussion The findings of the inventors provide a clear role, pathway and mechanism for selective ubiquitination in regulating developmental patterning. The Example presented evidence that TGFβ signaling can be controlled by ubiquitination of Smad signal transduction molecules, and that this activity has developmental consequences. Specifically, it was shown that Smurfl , a Hect family E3 ubiquitin ligase, selectively and directly interacts with BMP pathway-specific Smads, Smadl and Smad5, triggering their ubiquitination and degradation. Smurfl does not, however, interact with or affect TGFβ/activin pathway-specific Smad2, nor the common partner in Smad signal transduction complexes, Smad4. Furthermore, targeting of Smads by Hect ubiquitin ligases is not a general characteristic of E3 ligases since Nedd4 does not interact with Smads. Interestingly, the results showed Smurf 1 -mediated turnover of Smads is not affected by BMP signaling, suggesting that activated forms of Smadl or Smad5 are not required to serve as Smurfl substrates. Thus, Smurfl does not act downstream of activated Smads to turn off BMP signals, but rather controls the competence of cells to respond to BMPs by regulating the steady state level of Smad protein in the cell.The mSmurfl gene is expressed in early development, where we predict it will regulate the response of cells to BMP signals. Smurfl is present in adult tissues implying mSmurfl regulates late stage BMP signals. Perhaps these signals act in tissue stasis, or perhaps ongoing differentiation associated with growth. To a large degree the expression in developing mouse at neurula stage and thereafter is in good accord with the expression patterns seen in the frog embryo at similar stages. The phenotypic effects of Smurfl in Xenopus embryos point to selective ubiquitination as an important regulator of inductive signals during embryonic development. Smurfl dorsalizes mesoderm and neuralizes ectoderm by interfering with BMP signals that control patterning of these germ layers in normal development. Presently, regulation of BMP-dependent patterning is considered to be accomplished by secreted BMP -binding proteins, such as chordin, noggin and follistatin, which inhibit BMP signals extracellular ly by direct binding to BMP ligands. However, Smurfl provides a new mechanism to regulate BMP signals, which acts at the level of signal transduction, and presumably functions cell autonomously because Smurfl is an intracellular protein. Furthermore, unlike secreted BMP binding proteins, which have restricted ligand specificity, Smurfl provides broad inhibitory activity for BMP pathways because many type I receptors (ALK1, ALK2, ALK3, and ALK6) signal through Smadl and Smad5 (42). Thus, in the developing embryo Smurfl cooperates with extracellular BMP inhibitors to specify pattern formation. Whether Smurfl is regulated in some manner remains an open question.Of particular interest is the localization of Smurfl mRNA to the animal pole of the Xenopus egg and blastula. This region forms ectodermal tissues such as epidermis, cement gland and the nervous system, tissue fates which are controlled by the level of BMP signaling perceived by the cells. When animal pole ectoderm is deprived of endogenous BMP signals neural differentiation occurs spontaneously, but as the levels of BMP ligand applied to cells, or the amount of Smadl expressed within cells, are gradually increased, a spectrum of cell fates are progressively specified from neuronal, through cement glad then epidermis. Furthermore, reduced BMP signaling in the prospective ventral mesoderm of Xenopus embryos causes that tissue to be re-specified as dorsal mesoderm. Since Smurfl can regulate cell responsiveness to BMP signals it may function in both ectodermal and mesodermal fate specificity by controlling the level of Smadl or Smad5. High Smurfl levels may completely shut off the ability of a cell to respond to BMPs, but at intermediate or low levels Smurfl may modulate the magnitude of the BMP signal through the control of Smadl protein levels to alter the nature of the cellular response. Consequently, Smurfl may control cell fate determination in response to BMPs by establishing an intracellular moφhogenetic gradient of Smadl activity. In a variety of animals maternal mRNAs localized in eggs function as determinants of cell fate (43). In Xenopus, mRNAs encoding Vgl (a TGFβ member) and Brat/VgT are localized to the egg vegetal pole where they specify endoderm and mesoderm (44, 45). The results presented in this Example suggest that localized Smurfl in the egg animal pole may function as a determinant of ectodermal cell fate.An intriguing consequence of Smurfl overexpression in Xenopus animal caps is altered cell competence to respond to Smad2, the activin/TGFβ pathway specific Smad. Smurfl enhances the sensitivity of cells to a fixed level of ectopically expressed Smad2, so as Smurfl levels increase, the mesoderm that is induced becomes progressively more characteristic of the Spemann organizer, the most dorsal type of mesoderm. This response mimics the effects of what happens when the levels of Smad2 alone are elevated in animal caps. Thus, Smurfl can simultaneously change the competence of cells to respond to different TGFβ signals: it inhibits responses to the Smadl pathway while stimulating responses to the Smad2 pathway. The distinct effects of Smurfl on different TGFβ signaling pathways has important consequences for embryonic development, cell growth, tissue stasis and other biological functions regulated by the TGFβ superfamily.EXAMPLE 2: Characterization and Cloning of human Smurf2Human SmurfZ was identified and cloned using a Xenopus Smurfl sequence in an EST database. Two overlapping EST clones corresponding to hSmurfiZ were obtained and used to construct a full length sequence for hSmurfZ. Smurf2 is closely related to Smurfl, displaying approximately 75% homology to the amino acid sequence of hSmurfl . Smurf contains a C2 domain at the amino-terminus, followed by three WW domains and a HECT ubiquitin ligase domain (Fig. 12). SmurfZ is closely related to Smurfl, but possesses an extra WW domain downstream of the C2 domain (Fig. 13). SmurfZ was found to be expressed throughout early development and was present in most adult tissues, with lower levels in spleen and skeletal muscle (Figs.l4A and 14B). RT- PCR analysis further revealed that SmurfZ is expressed in a variety of cell lines that include PI 9, HepG2 and 293T.Isolation of Human and mouse Smurfl. Several overlapping human clones displaying similarity to Smurfl were identified from the expressed sequence tag (EST) database and a full-length version of SmurfZ was constructed by PCR using two overlapping EST clones. For northern blot analysis, a partial mouse SmurfZ cDNA clone encoding 225 amino acids of open reading frame including the stop codon and displaying 96% amino acid identity to human SmurfZ was identified in the EST database (I.M.A.G.E. clone ID 638876).Construction of Plasmids. For mammalian expression constructs of SmurfZ, the open reading frame was amplified by polymerase chain reaction (PCR) and was subcloned into pCMV5 in frame with an amino-terminal Flag or Myc tag (69). For Smurf2 WW domain deletions, amino acids 163-185 for DWW1, 257-279 for DWW2, and 303-325 for DWW3 were deleted. To generate the catalytically-inactive ubiquitin- ligase mutant of SmurfZ, cysteine 716 was replaced with alanine. To generate the Smad7 PY mutants, tyrosine 211 was replaced with alanine (Y211 A) or the PPPPY sequence between amino acid residues 206-212 was deleted (ΔPY). For TβRI-Flag, a Flag tag was introduced at the carboxy terminus of the receptor. All constructs were generated by PCR and confirmed by sequencing. The bacterial expression vectors, pET15-Smad7-HA and pGEX4T-l -SmurfZ, were generated using convenient restriction sites.Immunoprecipitation, Immunoblotting, and Affinity-labelling. For studies in mammalian cells, 293T and COS-1 cells were transiently transfected using calcium phosphate precipitation, or the DEAE-dextran method, respectively. Immunoprecipitation and immunoblotting were carried out using anti-HA monoclonal (12CA5, Boehringer), anti-HA rabbit polyclonal (Santa Cruz), anti-Myc monoclonal (9E10 ascites, Developmental Studies Hybridoma Bank), anti-Flag M2 monoclonal (Sigma) or anti- Smad7 rabbit polyclonal antibodies. For anti-Smad7 antibodies, rabbits were immunized with bacterially-produced GST-Smad7 encoding amino acids 202-260. After absoφtion of the antibody to either protein G or A-Sepharose, the precipitates were washed five times with TNTE 0.1% (50 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% Triton X-100), separated by SDS-PAGE, transferred to nitrocellulose and immunoblotted with the appropriate antibody. Detection was conducted using the appropriate horseradish peroxidase (HRP)-coηjugated sheep anti-mouse or anti-rabbit secondary antibodies and enhanced chemiluminescence (Amersham). Bacterially-produced His-Smad7-HA was incubated with either Ni2+-NTA beads (Qiagen) or with GST or GST-SmurfiZ -bound glutathione beads (Amersham), washed three times with TNTE (0.5% Triton X-100) and precipitates were analyzed by immunoblotting with anti-HA antibodies. For affinity- labelling, transfected COS-1 cells were incubated with 250 pM [125I]TGF-bl at 4 °C for 1 h, and receptors were cross-linked to ligand with DSS as described (70). The amount of TβRI bound to Smurf2 or Smad7 was quantified by phosphorimaging (Molecular Dynamics).Pulse-chase analysis and ubiquitination assay. COS-1 cells were transfected with the indicated constructs and two days post-transfection the cells were labelled for 15 min at 37 °C with 50 mCi/ml [35S]-methionine (Trans [35S]-label; Figure 2B) or 150 mCi/ml [35S] -methionine (Figure 5C and D) in methionine-free Dulbecco's Modified Eagle's Medium (DMEM). Cell layers were then washed two times and incubated in DMEM containing 10%> fetal bovine serum in the presence or absence of 30 mM lactacystin (obtained from E.J. Corey, Harvard University) or 0.4 mM chloroquine for the indicated times. At each time point, cell lysates were immunoprecipitated with anti-HA monoclonal antibodies, resolved by SDS-PAGE and visualized by autoradiography. Metabolically-labelled proteins were quantified by phosphorimaging. For the ubiquitination assay, 293T cells were transfected with HA-tagged ubiquitin and combinations of receptors, Smad7 and SmurfZ. Cell lysates were subjected to anti-Smad7 immunoprecipitation and immunoprecipitates were boiled in 1% SDS for 5 min, diluted with TNTE 0.1% and reprecipitated with anti-Smad7 antibodies before anti-HA immunoblotting.Subcellular localization by Immunofluorescence Deconvolution Microscopy. MvlLu cells, plated on gelatin-coated Permanox chamber slides (Nunc), were transfected with the indicated constructs by the calcium phosphate precipitation method. The cells were fixed, permeabilized, and reacted with the primary and secondary antibodies as described (69). Images were obtained using the Olympus 1X70 inverted microscope equipped with fluorescence optics and Deltavision deconvolution microscopy software (Applied Precision).Transcriptional Response Assay. HepG2 cells were transiently transfected using the calcium phosphate DNA precipitation method with CMV-βgal, 3TP-Lux reporter construct and Smad7-HA constructs as indicated. Total DNA was kept constant by the addition of pCMV5 empty vector. The next day, cells were incubated overnight with or without 100 pM TGFβ. Luciferase activity was measured using the luciferase assay system (Promega) in a Berthold Lumat LB 96V luminometer and was normalized to β-galactosidase activity. ResultsSmurfl does not regulate Smad steady-state levels. Smads were expressed in 293T cells in the absence or presence of SmurfZ. Suφrisingly, unlike Smurfl which targets Smadl for ubiquitin-mediated proteolysis, SmurfZ expression did not alter the steady-state levels of Smads 1, 2, 4 or 7 (Fig. 15A). To determine whether SmurfZ might associate with any of these Smads, the cell lysates were subjected to anti-SmurfZ immunoprecipitation and associated Smads examined by immunoblotting. In cells coexpressing Smadl, 2, or 4, no Smads were found to coprecipitate with Smurf2 under these conditions (Fig. 15B). In contrast, in cells expressing Smad7, a strong interaction between SmurfZ and Smad7 was detected. To confirm that Smurf expression did not alter Smad7 turnover, we also performed pulse-chase analysis of Smad7. Smad7 expressed in the absence or presence of SmurfZ displayed a similar half-life of approximately 4 h (Fig. 15C). To characterize the Smad7-SmurfZ association, GST- SmurfZ fusion protein was purified from bacteria and incubated with bacterially produced Smad7. Under these in vitro conditions Smad7 bound efficiently to Smurf , indicating that SmurfZ associates directly with Smad7 (Fig. 15D). We also analyzed the determinants on Smad7 and SmurfZ that mediate their interaction. Smad7 possesses a PPXY sequence (PY motif) in its linker region, which can mediate interaction with WW domains such as those found in Smurf (71). The Smad7 PY motif was altered by changing the tyrosine residue to alanine (Smad7(Y211 A)) or by deleting the entire motif (Smad7(ΔPY)). Analysis in 293 T cells of Smurf2 binding to either Smad7 mutant revealed that the interaction with SmurfZ was reduced but not entirely abolished (Fig. 15E). This suggests that the PY motif makes an important contribution to the Smad7-Smurf2 interaction, however, it is not the sole determinant, and other regions in Smad7 may also contribute to their association. We also made mutants of SmurfZ in which each of the three WW domains were deleted. The interaction of Smad7 with a SmurfZ mutant that lacked the first WW domain was comparable to wild type SmurfZ, however deletion of either WW2 or WW3 abolished any detectable interaction with Smad7 (Fig. 15F). Thus, the WW2 and WW3 domains in SmurfZ are both required to mediate binding to Smad7. Together, these results show that SmurfZ binds directly to Smad7 via its WW2 and WW3 domains but that it does not target Smad7 for ubiquitin-mediated proteolysis in the absence of TGFβ signalling. 62Smadl recruits Smurfl into a complex with the TGFβ receptors. Smad7 binds heteromeric complexes of TGFβ type II (TβRII) and type I (TBRI) receptors through interactions with the activated type I receptor subunit (72, 56). The constitutive association between Smad7 and Smurf thus raised the interesting possibility that Smad7 might function to recruit SmurfZ to the TGFβ receptor complex. To test this, we expressed TGFβ receptors in COS-1 cells in the presence and absence of Smad7 and SmurfZ. Receptors were then labelled by crosslinking to [125I]-TGFβ. Affinity-labelled receptor complexes that co-precipitated with Smurf were visualized by autoradiography. In the absence or presence of Smad7, little or no TGFβ receptor complexes were found to co-precipitate with wild type Smurf2 (Fig. 16). A SmurfZ catalytic mutant was constructed in SmurfZ in which cysteine 716 was converted to an alanine residue (C716A). This mutation targeted the cysteine residue in the HECT ubiquitin-ligase domain that is thought to form a thiolester bond with ubiquitin. When Smurf2(C716A) was expressed alone we detected a slight interaction with the TGFβ receptors (Fig. 16). However in the presence of Smad7 the interaction of Smurf2(C716A) with the receptors was dramatically enhanced. Thus, Smad7 mediates the interaction of SmurfZ with the TGFβ receptors.We also examined Smad7 bound to the receptors. Smad7 binds heteromeric TGFβ receptor complexes by interacting with the activated type I receptor subunit (72, 56). In the presence of wild type Smurf2 we observed a strong decrease in the amount of TGFβ receptor complexes that co-precipitated with Smad7 (Fig. 16, lane 3). This correlated with a decrease in the total amount of type I receptor present in these transfectants. Since Smad7 binds to receptor complexes via interactions with the activated type I receptor, these results suggest that SmurfZ decreases the levels of Smad7-bound receptor complexes. Consistent with this notion, when Smad7 was co-expressed with the catalytically inactive mutant of SmurfZ, a decrease in Smad7-bound receptor levels was not observed (Fig. 16, lane 5). These results indicate that the catalytic activity of SmurfZ mediates the down-regulation of TGFβ receptors that are bound to Smad7.Smadl controls the subcellular localization of Smurfl. Smad7 resides in the nucleus and upon activation of TGFβ signalling is exported into the cytoplasm where it binds to TGFβ receptors through interaction with the receptor I subunit (73). To investigate whether Smad7 recruited SmurfZ to the receptors in intact cells, we determined whether Smad7 might regulate Smurf localization. For this, we expressed TβRII, TβRI 63 and SmurfZ(C716A) in the absence or presence of Smad7 and examined the subcellular distribution of the appropriate protein by immunofluorescence microscopy.Smad7 was localized to the nucleus, but in the presence of signalling was found predominantly in the cytoplasm, where it also colocalized with the transiently expressed TGFβ receptors in a punctate pattern. This distribution of TGFβ receptors has been observed previously by us and others (74-76). SmurfZ was similarly found predominantly in the nucleus, but this localization was not altered in the presence of TGFβ signalling. However, when Smad7 was coexpressed, the subcellular distribution of SmurfZ was dramatically altered and the protein was found predominantly outside of the nucleus and was extensively colocalized with the TGFβ receptors. These results suggest that Smad7 expression leads to export of SmurfZ from the nucleus and recruitment of SmurfZ to the TGFβ receptor complex.Smurfl induces degradation of TGFβ receptors and Smadl. The strong decrease in Smad7-associated receptors in the presence of wild type but not mutant SmurfZ suggested the possibility that SmurfZ might catalyze degradation of Smad7 -bound receptor complexes. To analyze SmurfZ-dependent turnover of TGFβ receptor complexes we coexpressed TβRII and TβRI in 293T cells. Under these conditions TβRII and TβRI assemble efficiently into functional heteromeric complexes, allowing us to investigate the turnover of the entire receptor pool. We first investigated steady-state levels of the receptors in cells expressing SmurfZ (Fig. 17A) and observed that SmurfZ had minimal effects on the steady-state levels of type II or type I receptors when the receptors were expressed either alone or together. However, in the presence of wild type Smad7, increasing Smurf2 expression led to a strong decrease in the steady-state levels of the type I receptor (Fig. 17B). In contrast, Smurf2(C716A) had no effect. Levels of type II receptor were also decreased upon expression of wild type Smurf , although to a lesser extent than that observed for the type I receptor. This difference may be due to the fact that Smad7 binds receptor complexes via the activated type I receptor and does not interact with TβRII alone. We also tested whether SmurfZ targets a constitutively active version of the type I receptor, TβRI(T204D). This receptor mediates TGFβ signalling in the absence of the type II receptor and also binds Smad7 (72). Similar to receptor complexes, wild type SmurfZ, but not Smurf2(C716A) caused a decrease in the steady-state levels of the activated type I receptor (Fig. 17B). To confirm that changes in receptor steady- state „ „64 levels reflected alterations in receptor turnover, we analyzed by pulse-chase analysis the half-life of TβRII and TβRI (Fig. 17C). Analysis of the type II receptor revealed that the newly synthesized protein had a half-life of approximately 1 h. In contrast TβRI was considerably more stable and displayed a half-life of approximately 4-6 h. Furthermore, the half-life of the type I receptor was unchanged when either Smad7 or SmurfZ were expressed individually with the receptors. However, when SmurfZ and Smad7 were co- expressed together with the TGFβ receptor complex, the half-life of the type I receptor was decreased to approximately 1 h. Thus, Smad7 and Smurf enhance the turnover of the type I receptor. Ubiquitin-mediated proteolysis of membrane receptors can be mediated by both the proteasome and lysosome (60). To test whether SmurfZ-dependent enhancement of TGFβ receptor degradation was dependent on proteasome and lysosome function we assessed the turnover of receptors in the presence and absence of lactacystin and chloroquine, which inhibit protein degradation by the proteasome and lysosome, respectively. Pulse-chase analysis of receptors revealed that each inhibitor on its own caused stabilization of a subset of the total TGFβ receptor pool (Fig. 17D). These results suggest that both the proteasome and the lysosome contribute to the enhanced turnover of the receptors that is observed in the presence of Smad7 and SmurfZ.In the course of these analyses of the TGFβ receptor we also evaluated Smad7 protein levels. In the absence of TGFβ receptor complexes Smad7 steady-state level and turnover was unaffected by SmurfZ (see Fig. 15). However, in the presence of TGFβ receptor complexes, Smad7 steady-state levels and half-life were decreased by SmurfZ expression (Figs. 17B and 17C, respectively). Furthermore, this decrease in Smad7 was dependent on the catalytic activity of the SmurfZ HECT domain, since expression of the Smurf2(C716A) mutant did not alter Smad7 levels. Smad7 turnover was also stabilized by lactacystin and chloroquine, suggesting that like the receptor complex, Smad7 is degraded by both proteasomal and lysosomal pathways. Thus, in the presence of TGFβ signalling SmurfZ induces degradation of Smad7, possibly by targeting the entire receptor-Smad7 complex.To investigate ubiquitination of the receptors and Smad7, we expressed anHA epitope-tagged version of ubiquitin and evaluated ubiquitin-conjugates of TβRII, TβRI or Smad7 by immunoprecipitation followed by immunoblotting. To ensure specificity, immunoprecipitated samples were boiled in SDS and then reprecipitated with the appropriate antibody prior to immunoblotting. Analysis of Smad7 ubiquitination revealed that in the absence or presence of SmurfZ there was little ubiquitin conjugated to the protein (Fig. 17E). However, when SmurfZ was coexpressed with Smad7 and the TGFβ receptors, we observed high molecular weight ubiquitin conjugates of Smad7 that were not detected when the catalytic mutant of Smurf2 was used. In contrast to Smad7, we were unable to detect significant SmurfiZ-dependent ubiquitination of the type I receptor. Since receptor turnover is increased in the presence of SmurfZ and Smad7, the inability to detect ubiquitin-conjugated receptors may reflect rapid degradation of the receptors that may occur once they are ubiquitinated. Alternatively, ubiquitination of Smad7 may serve as the signal that targets the entire receptor- Smad7 complex to the proteasome. Together these results show that Smad7-dependent recruitment of SmurfZ to the TGFβ receptor leads to proteasome and lysosome-mediated degradation of TGFβ receptor complexes and Smad7.Association of Smurfl enhances the inhibitory activity of Smadl. Our studies indicate that SmurfZ may be recruited to the TGFβ receptor complex via Smad7 and target the receptors for degradation. This suggests that ubiquitin-mediated degradation may contribute to the inhibitory activity of Smad7 in the TGFβ pathway. To test this we first investigated whether Smad7(Y211 A), which displays poor interactions with Smurf2 (see Fig. 15D), has an altered ability to recruit SmurfZ to the TGFβ receptor. For this, wild type Smad7 or Smad7(Y211 A) were coexpressed with TGFβ receptors in the presence or absence of Smurf2(C716A) and receptor interaction was measured by analyzing affinity labelled receptors that coprecipitated with either Smad7 or SmurfZ. The interaction of TGFβ receptor complexes with Smad7(Y211 A) was comparable to that observed with wild type Smad7 (Fig. 18A), demonstrating that this mutation in the PY motif does not affect Smad7 interaction with the TGFβ receptor. However, the ability of Smurf2(C716A) to associate with TGFβ receptors was substantially reduced in the presence of mutant Smad7, correlating with the reduced efficiency observed for the interaction between Smurf2 and Smad7(Y211 A) described above. Next we investigated whether Smad7(Y211 A) had an altered capacity to inhibit TGFβ signalling in HepG2 cells, which express endogenous SmurfZ. For this, we assessed TGFβ signalling using the well characterized 3TP-lux reporter construct. Expression of wild type Smad7 strongly reduced TGFb-dependent induction of this reporter (Fig. 18B). In contrast, the 66Smad7(Y211A) mutant had a substantially reduced inhibitory activity, despite its ability to interact efficiently with the TGFβ receptor complex. This suggests that binding of SmurfZ to Smad7 enhances Smad7 inhibitory activity towards the TGFβ signalling pathway. Previous studies have shown that Smad7 binding to TGFβ receptor complexes prevents access of Smad2 to the receptors. Since Smad7(Y211 A) interacts efficiently with the TGFβ receptor, we sought to determine whether the mutant protein might retain inhibitory activity to the TGFβ pathway at higher levels of expression. To examine this, we compared the relative efficiency of Smad7 versus Smad7(Y211 A) in blocking TGFβ signalling by varying the amount of Smad7 expression. Wild type Smad7 potently inhibited TGFβ signalling at the lowest doses tested, whereas Smad7(Y211A) was much less efficient (Fig. 18C). However, at the highest dose tested, Smad7(Y211A) was capable of inhibiting TGFβ responsiveness. These results indicate that Smad7(Y21 1 A) retains some inhibitory activity, probably through its ability to bind to the receptor and prevent access of Smad2 or Smad3. Together these data suggest that SmurfZ cooperates with Smad7 to promote its inhibitory activity.Discussion This Example confirms the identity of a new ubiquitin ligase, SmurfZ, that functions in partnership with Smad7 to target the TGFβ receptor for degradation. SmurfZ bound TGFβ receptors very weakly and was unable to affect turnover of the receptors. However, in presence of Smad7, SmurfZ formed efficient complexes with the heteromeric TGFβ receptor and targeted the receptors for degradation. Since Smad7 binds directly to SmurfZ and also associates with the TGFβ receptor complex, these results indicated that Smad7 functions as an adaptor protein that mediates the interaction of Smurf with the TGFβ receptor complex (Fig. 18D). Consistent with this, mutations in the Smad7 PY motif that disrupted Smad7-Smurf2 interaction also interfered with the ability of Smad7 to recruit SmurfZ into a complex with the receptor. This mutant of Smad7 was also compromised in its ability to block TGFβ signalling, indicating that SmurfZ plays a role in mediating Smad7 inhibitory function. Since Smad7 competes with R-Smads for binding to the activated TGFβ receptor, this cooperation may be particularly important when Smad7 is expressed transiently or at low levels (53-58). By targeting receptors for degradation, Smurf can thus provide a mechanism for permanent inactivation of the Smad7-bound receptor complex. Concomitant with SmurfZ-dependent degradation of 67TGFβ receptors, we also observed degradation of Smad7 that was dependent on TGFβ signalling.The present invention is not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and the accompanying figures. Such modifications are intended to fall within the scope of the appended claims.It is further to be understood that all base sizes or amino acid sizes, and all molecular weight or molecular mass values are approximate, and are provided for description.All patents, patent applications, publications, and other materials cited herein are hereby incoφorated herein reference in their entireties. References Cited

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WHAT IS CLAIMED IS:

1. An isolated Smurf protein.

2. The Smurf protein of claim 1, which is human.

3. The Smurf protein of claim 1 , which is a Smurfl .

4. The Smurfl of claim 3 which has a mutation corresponding to C710A.

5. The Smurfl of claim 3, which comprises at least 10 contiguous amino acid residues as depicted in a sequence of SEQ ID NO:2.

6. The Smurfl of claim 3, which comprises an amino acid sequence as depicted in SEQ ID NO:2.

7. The Smurf protein of claim 1, which is a Smurf2.

8. The Smurf2 of claim 7 which has a mutation corresponding to

C716A.

9. The SmurfZ protein of claim 7, which comprises at least 10 contiguous amino acid residues as depicted in SEQ ID NO:4.

10. The SmurfZ protein of claim 7, which comprises an amino acid sequence as depicted in SEQ ID NO:4.

11. An isolated nucleic acid encoding the Smurf protein of claim 1.

12. The nucleic acid of claim 11, wherein the Smurf protein is a human Smurf protein. /8 13. The nucleic acid of claim 11 , wherein the Smurf protein is a Smurfl .

14. The nucleic acid of claim 13 which has a mutation corresponding to C710A.

15. The nucleic acid of claim 13, wherein the Smurfl comprises at least 10 contiguous amino acid residues as depicted in a sequence of SEQ ID NO:2.

16. The nucleic acid of claim 13, wherein the Smurfl comprises an amino acid sequence as depicted in SEQ ID NO:2.

17. The nucleic acid of claim 16, which has a nucleotide sequence as depicted in SEQ ID NO: 1.

18. The nucleic acid of claim 11 , wherein the Smurf protein is a SmurfZ.

19. The nucleic acid of claim 18 which has a mutation corresponding to

C716A.

20. The nucleic acid of claim 18, wherein the Smurf2 protein comprises at least 10 contiguous amino acid residues as depicted in SEQ ID NO:4.

21. The nucleic acid of claim 18, wherein the SmurfZ protein comprises an amino acid sequence as depicted in SEQ ID NO:4.

22. The nucleic acid of claim 18, which has a nucleotide sequence as depicted in SEQ ID NO:3.

23. A vector comprising the nucleic acid of claim 11.

24. A host cell comprising the vector of claim 23. 79

25. A method for producing a Smurf protein, comprising growing the host cell of claim 23 under conditions that permit expression of Smurf protein from the vector.

26. A method for producing a Smurf protein, comprising growing the host cell of claim 24 under conditions that permit expression of Smurf protein from the vector.

27. A transgenic non-human animal that expresses a human Smurf protein.

28. A method for inhibiting a bone moφhogenic protein or tumor growth factor-beta activation pathway in a cell, which method comprises permitting the cell to grow under conditions that permit expression of Smurf from the vector of claim 23 introduced into the cell.

29. A method for inhibiting a bone moφhogenic protein or tumor growth factor-beta activation pathway in a cell, which method comprises permitting the cell to grow under conditions that permit expression of Smurf from the vector of claim 24 introduced into the cell.

30. A method for promoting a bone moφhogenic protein or tumor growth factor-beta activation pathway in a cell, which method comprises suppressing expression of endogenous Smurf in the cell.

31. A method of screening for a modulator of Smurf activity, which method comprises detecting modulation of Smurf activity in the presence of a test compound relative to Smurf activity in the absence of the test compound.

32. The method according to claim 31 , wherein the Smurf activity is ubiquitination of a Smad protein in a host cell. 80 33. The method according to claim 31 , wherein the Smurf activity is interaction of a Smurf WW domain with a PPXY domain of a Smad protein.

34. The method according to claim 33, wherein the test compound is screened for the ability to inhibit the interaction.

35. An antibody that specifically binds to Smurf protein.

36. An oligonucleotide or nucleic acid that specifically hybridizes under highly stringent conditions to an nucleic acid having a sequence encoding Smurf.